LOCUS       DAZPLM010000001        75794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPLM010000001 DAZPLM010000000
VERSION     DAZPLM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.001"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(95..1345)
                     /gene="icd"
                     /locus_tag="ACHVC7_000001"
     CDS             complement(95..1345)
                     /gene="icd"
                     /locus_tag="ACHVC7_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR2114164.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1517..2170
                     /gene="rluE"
                     /locus_tag="ACHVC7_000002"
     CDS             1517..2170
                     /gene="rluE"
                     /locus_tag="ACHVC7_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR2114165.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2180..2641
                     /gene="nudJ"
                     /locus_tag="ACHVC7_000003"
     CDS             2180..2641
                     /gene="nudJ"
                     /locus_tag="ACHVC7_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR2114166.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2695..3801
                     /gene="mnmA"
                     /locus_tag="ACHVC7_000004"
                     /gene_synonym="trmU"
     CDS             2695..3801
                     /gene="mnmA"
                     /locus_tag="ACHVC7_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR2114167.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3837..4478
                     /gene="hflD"
                     /locus_tag="ACHVC7_000005"
     CDS             3837..4478
                     /gene="hflD"
                     /locus_tag="ACHVC7_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR2114168.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4482..5852
                     /gene="purB"
                     /locus_tag="ACHVC7_000006"
     CDS             4482..5852
                     /gene="purB"
                     /locus_tag="ACHVC7_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR2114169.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6020..6691
                     /gene="phoP"
                     /locus_tag="ACHVC7_000007"
     CDS             6020..6691
                     /gene="phoP"
                     /locus_tag="ACHVC7_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR2114170.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6691..8151
                     /gene="phoQ"
                     /locus_tag="ACHVC7_000008"
     CDS             6691..8151
                     /gene="phoQ"
                     /locus_tag="ACHVC7_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR2114171.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8227..9348
                     /gene="roxA"
                     /locus_tag="ACHVC7_000009"
                     /gene_synonym="ycfD"
     CDS             8227..9348
                     /gene="roxA"
                     /locus_tag="ACHVC7_000009"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR2114172.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9397..10623)
                     /gene="pepT"
                     /locus_tag="ACHVC7_000010"
     CDS             complement(9397..10623)
                     /gene="pepT"
                     /locus_tag="ACHVC7_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR2114173.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10873..12009
                     /gene="potA"
                     /locus_tag="ACHVC7_000011"
     CDS             10873..12009
                     /gene="potA"
                     /locus_tag="ACHVC7_000011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR2114174.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11993..12850
                     /gene="potB"
                     /locus_tag="ACHVC7_000012"
     CDS             11993..12850
                     /gene="potB"
                     /locus_tag="ACHVC7_000012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR2114175.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12847..13641
                     /gene="potC"
                     /locus_tag="ACHVC7_000013"
     CDS             12847..13641
                     /gene="potC"
                     /locus_tag="ACHVC7_000013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR2114176.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13638..14684
                     /gene="potD"
                     /locus_tag="ACHVC7_000014"
     CDS             13638..14684
                     /gene="potD"
                     /locus_tag="ACHVC7_000014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR2114177.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14840..15661)
                     /gene="cobB"
                     /locus_tag="ACHVC7_000015"
     CDS             complement(14840..15661)
                     /gene="cobB"
                     /locus_tag="ACHVC7_000015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR2114178.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15677..16588)
                     /gene="nagK"
                     /locus_tag="ACHVC7_000016"
     CDS             complement(15677..16588)
                     /gene="nagK"
                     /locus_tag="ACHVC7_000016"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR2114179.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16617..17861)
                     /gene="lolE"
                     /locus_tag="ACHVC7_000017"
     CDS             complement(16617..17861)
                     /gene="lolE"
                     /locus_tag="ACHVC7_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR2114180.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17861..18562)
                     /gene="lolD"
                     /locus_tag="ACHVC7_000018"
     CDS             complement(17861..18562)
                     /gene="lolD"
                     /locus_tag="ACHVC7_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR2114181.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18555..19754)
                     /gene="lolC"
                     /locus_tag="ACHVC7_000019"
     CDS             complement(18555..19754)
                     /gene="lolC"
                     /locus_tag="ACHVC7_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR2114182.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20016..21089
                     /locus_tag="ACHVC7_000020"
     CDS             20016..21089
                     /locus_tag="ACHVC7_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR2114183.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21217..24663
                     /gene="mfd"
                     /locus_tag="ACHVC7_000021"
     CDS             21217..24663
                     /gene="mfd"
                     /locus_tag="ACHVC7_000021"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR2114184.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24807..25763
                     /gene="ldtC"
                     /locus_tag="ACHVC7_000022"
                     /pseudo
     CDS             24807..25763
                     /gene="ldtC"
                     /locus_tag="ACHVC7_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25846..26103)
                     /gene="bhsA"
                     /locus_tag="ACHVC7_000023"
                     /gene_synonym="ycfR"
     CDS             complement(25846..26103)
                     /gene="bhsA"
                     /locus_tag="ACHVC7_000023"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR2114185.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26344..26976
                     /gene="comR"
                     /locus_tag="ACHVC7_000024"
     CDS             26344..26976
                     /gene="comR"
                     /locus_tag="ACHVC7_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR2114186.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27038..27577)
                     /locus_tag="ACHVC7_000025"
     CDS             complement(27038..27577)
                     /locus_tag="ACHVC7_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR2114187.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(27804..29108)
                     /gene="ndh"
                     /locus_tag="ACHVC7_000026"
     CDS             complement(27804..29108)
                     /gene="ndh"
                     /locus_tag="ACHVC7_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR2114188.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29516..30058)
                     /gene="ycfP"
                     /locus_tag="ACHVC7_000027"
     CDS             complement(29516..30058)
                     /gene="ycfP"
                     /locus_tag="ACHVC7_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR2114189.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30081..31106)
                     /gene="nagZ"
                     /locus_tag="ACHVC7_000028"
     CDS             complement(30081..31106)
                     /gene="nagZ"
                     /locus_tag="ACHVC7_000028"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR2114190.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31117..31941)
                     /gene="thiK"
                     /locus_tag="ACHVC7_000029"
     CDS             complement(31117..31941)
                     /gene="thiK"
                     /locus_tag="ACHVC7_000029"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR2114191.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31922..32563)
                     /gene="lpoB"
                     /locus_tag="ACHVC7_000030"
     CDS             complement(31922..32563)
                     /gene="lpoB"
                     /locus_tag="ACHVC7_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR2114192.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32577..32954)
                     /locus_tag="ACHVC7_000031"
     CDS             complement(32577..32954)
                     /locus_tag="ACHVC7_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR2114193.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32957..33316)
                     /gene="hinT"
                     /locus_tag="ACHVC7_000032"
     CDS             complement(32957..33316)
                     /gene="hinT"
                     /locus_tag="ACHVC7_000032"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR2114194.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            33650..35839
                     /gene="fhuE"
                     /locus_tag="ACHVC7_000033"
     CDS             33650..35839
                     /gene="fhuE"
                     /locus_tag="ACHVC7_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR2114195.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35899..37332)
                     /gene="ptsG"
                     /locus_tag="ACHVC7_000034"
     CDS             complement(35899..37332)
                     /gene="ptsG"
                     /locus_tag="ACHVC7_000034"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR2114196.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(37627..38424)
                     /locus_tag="ACHVC7_000035"
     CDS             complement(37627..38424)
                     /locus_tag="ACHVC7_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2114197.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38435..39439)
                     /gene="holB"
                     /locus_tag="ACHVC7_000036"
     CDS             complement(38435..39439)
                     /gene="holB"
                     /locus_tag="ACHVC7_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR2114198.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39436..40077)
                     /gene="tmk"
                     /locus_tag="ACHVC7_000037"
     CDS             complement(39436..40077)
                     /gene="tmk"
                     /locus_tag="ACHVC7_000037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2114199.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40067..41089)
                     /gene="yceG"
                     /locus_tag="ACHVC7_000038"
     CDS             complement(40067..41089)
                     /gene="yceG"
                     /locus_tag="ACHVC7_000038"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR2114200.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41092..41901)
                     /gene="pabC"
                     /locus_tag="ACHVC7_000039"
     CDS             complement(41092..41901)
                     /gene="pabC"
                     /locus_tag="ACHVC7_000039"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR2114201.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(42021..43262)
                     /gene="fabF"
                     /locus_tag="ACHVC7_000040"
     CDS             complement(42021..43262)
                     /gene="fabF"
                     /locus_tag="ACHVC7_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR2114202.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43350..43586)
                     /gene="acpP"
                     /locus_tag="ACHVC7_000041"
     CDS             complement(43350..43586)
                     /gene="acpP"
                     /locus_tag="ACHVC7_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR2114203.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(43797..44531)
                     /gene="fabG"
                     /locus_tag="ACHVC7_000042"
     CDS             complement(43797..44531)
                     /gene="fabG"
                     /locus_tag="ACHVC7_000042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR2114204.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44544..45473)
                     /gene="fabD"
                     /locus_tag="ACHVC7_000043"
     CDS             complement(44544..45473)
                     /gene="fabD"
                     /locus_tag="ACHVC7_000043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR2114205.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45489..46442)
                     /gene="fabH"
                     /locus_tag="ACHVC7_000044"
     CDS             complement(45489..46442)
                     /gene="fabH"
                     /locus_tag="ACHVC7_000044"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR2114206.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46510..47580)
                     /gene="plsX"
                     /locus_tag="ACHVC7_000045"
     CDS             complement(46510..47580)
                     /gene="plsX"
                     /locus_tag="ACHVC7_000045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR2114207.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(47661..47834)
                     /gene="rpmF"
                     /locus_tag="ACHVC7_000046"
     CDS             complement(47661..47834)
                     /gene="rpmF"
                     /locus_tag="ACHVC7_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR2114208.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(47886..48407)
                     /gene="yceD"
                     /locus_tag="ACHVC7_000047"
     CDS             complement(47886..48407)
                     /gene="yceD"
                     /locus_tag="ACHVC7_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR2114209.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48606..49190
                     /gene="yceF"
                     /locus_tag="ACHVC7_000048"
     CDS             48606..49190
                     /gene="yceF"
                     /locus_tag="ACHVC7_000048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR2114210.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49302..50261)
                     /gene="rluC"
                     /locus_tag="ACHVC7_000049"
     CDS             complement(49302..50261)
                     /gene="rluC"
                     /locus_tag="ACHVC7_000049"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR2114211.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            50834..54019
                     /gene="rne"
                     /locus_tag="ACHVC7_000050"
     CDS             50834..54019
                     /gene="rne"
                     /locus_tag="ACHVC7_000050"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR2114212.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54214..55167)
                     /gene="flgL"
                     /locus_tag="ACHVC7_000051"
     CDS             complement(54214..55167)
                     /gene="flgL"
                     /locus_tag="ACHVC7_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR2114213.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55179..56822)
                     /gene="flgK"
                     /locus_tag="ACHVC7_000052"
     CDS             complement(55179..56822)
                     /gene="flgK"
                     /locus_tag="ACHVC7_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR2114214.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(56888..57829)
                     /gene="flgJ"
                     /locus_tag="ACHVC7_000053"
     CDS             complement(56888..57829)
                     /gene="flgJ"
                     /locus_tag="ACHVC7_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR2114215.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(57829..58926)
                     /gene="flgI"
                     /locus_tag="ACHVC7_000054"
     CDS             complement(57829..58926)
                     /gene="flgI"
                     /locus_tag="ACHVC7_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR2114216.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(58938..59636)
                     /gene="flgH"
                     /locus_tag="ACHVC7_000055"
     CDS             complement(58938..59636)
                     /gene="flgH"
                     /locus_tag="ACHVC7_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR2114217.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(59689..60461)
                     /gene="flgG"
                     /locus_tag="ACHVC7_000056"
                     /pseudo
     CDS             complement(59689..60461)
                     /gene="flgG"
                     /locus_tag="ACHVC7_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(60633..61388)
                     /gene="flgF"
                     /locus_tag="ACHVC7_000057"
     CDS             complement(60633..61388)
                     /gene="flgF"
                     /locus_tag="ACHVC7_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR2114218.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61408..62613)
                     /gene="flgE"
                     /locus_tag="ACHVC7_000058"
     CDS             complement(61408..62613)
                     /gene="flgE"
                     /locus_tag="ACHVC7_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR2114219.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(62638..63333)
                     /gene="flgD"
                     /locus_tag="ACHVC7_000059"
     CDS             complement(62638..63333)
                     /gene="flgD"
                     /locus_tag="ACHVC7_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR2114220.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63345..63749)
                     /gene="flgC"
                     /locus_tag="ACHVC7_000060"
     CDS             complement(63345..63749)
                     /gene="flgC"
                     /locus_tag="ACHVC7_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR2114221.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(63753..64169)
                     /gene="flgB"
                     /locus_tag="ACHVC7_000061"
     CDS             complement(63753..64169)
                     /gene="flgB"
                     /locus_tag="ACHVC7_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR2114222.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64324..64983
                     /gene="flgA"
                     /locus_tag="ACHVC7_000062"
     CDS             64324..64983
                     /gene="flgA"
                     /locus_tag="ACHVC7_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR2114223.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65059..65352
                     /gene="flgM"
                     /locus_tag="ACHVC7_000063"
     CDS             65059..65352
                     /gene="flgM"
                     /locus_tag="ACHVC7_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR2114224.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65357..65773
                     /gene="flgN"
                     /locus_tag="ACHVC7_000064"
     CDS             65357..65773
                     /gene="flgN"
                     /locus_tag="ACHVC7_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR2114225.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(65813..67348)
                     /gene="murJ"
                     /locus_tag="ACHVC7_000065"
     CDS             complement(65813..67348)
                     /gene="murJ"
                     /locus_tag="ACHVC7_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR2114226.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSLAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67458..68381)
                     /locus_tag="ACHVC7_000066"
     CDS             complement(67458..68381)
                     /locus_tag="ACHVC7_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2114227.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68383..69030)
                     /locus_tag="ACHVC7_000067"
     CDS             complement(68383..69030)
                     /locus_tag="ACHVC7_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR2114228.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69041..69625)
                     /gene="rimJ"
                     /locus_tag="ACHVC7_000068"
     CDS             complement(69041..69625)
                     /gene="rimJ"
                     /locus_tag="ACHVC7_000068"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR2114229.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            69861..71069
                     /gene="mdtH"
                     /locus_tag="ACHVC7_000069"
     CDS             69861..71069
                     /gene="mdtH"
                     /locus_tag="ACHVC7_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR2114230.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71133..71780
                     /gene="grxB"
                     /locus_tag="ACHVC7_000070"
     CDS             71133..71780
                     /gene="grxB"
                     /locus_tag="ACHVC7_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR2114231.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            71914..72474
                     /locus_tag="ACHVC7_000071"
     CDS             71914..72474
                     /locus_tag="ACHVC7_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2114232.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72580..73626
                     /gene="pyrC"
                     /locus_tag="ACHVC7_000072"
     CDS             72580..73626
                     /gene="pyrC"
                     /locus_tag="ACHVC7_000072"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR2114233.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            73700..73945
                     /gene="dinI"
                     /locus_tag="ACHVC7_000073"
     CDS             73700..73945
                     /gene="dinI"
                     /locus_tag="ACHVC7_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR2114234.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74235..74489
                     /gene="bssS"
                     /locus_tag="ACHVC7_000074"
     CDS             74235..74489
                     /gene="bssS"
                     /locus_tag="ACHVC7_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR2114235.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74604..75722
                     /gene="solA"
                     /locus_tag="ACHVC7_000075"
     CDS             74604..75722
                     /gene="solA"
                     /locus_tag="ACHVC7_000075"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR2114236.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18422 a        20166 c        18961 g        18245 t
ORIGIN      
        1 atataaatat gctcgatatg ctattaataa tatccacata aataaaacaa cgggcgtgtt
       61 atacgcccgt ttcaatattt aactcatgga gagattacat gttttcgatg atcgcttcac
      121 caaactctga acatttcagc agcttagcgc cttccatcag acgttcaaag tcataagtta
      181 cggttttcgc gttgatagca ccttccatac ctttaacaat taaatcagcc gcttcagtcc
      241 accccatatg gcgcagcatc atctcagcgg agaggatgat agagcctgga tttactttgt
      301 cctgaccggc atatttcggc gcagtaccgt gggtggcttc aaacagggcg cattcgtcac
      361 cgatgtttgc gccaggggcg ataccgatac cgccaacctg cgctgccagg gcgtcagaaa
      421 tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga cgcagcagga
      481 tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg ccggtgttcg
      541 ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac tcttcacgcg
      601 ccagctggta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg ttgcctttgt
      661 gcaccagagt cacagagtca cgatcgttag caattgcgta ttcgatcgct gcacgaacca
      721 gacgtttggt gccttcttcc gaacacggct taataccgat accacaatgt tccgggaagc
      781 gaattttctt cacccccatc tcttcacgca ggaatttaat cactttctcg gcgtcggcag
      841 agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg aagataacca
      901 tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ctgatagtaa cgtaccggac
      961 gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag cgaataccgc
     1021 caccaacagg agtggtcagc ggacctttaa tggcaacgcg atattcacga atcagatcaa
     1081 gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt tcaccggtgt
     1141 aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact gcagcgtcga
     1201 ccactttcag catggctggg gttacatcta caccgattcc atcaccttca atgtaaggga
     1261 taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc ttgccttgtg
     1321 ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg tttgctcgtc
     1381 tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga gcaatttttt
     1441 gttaatgatt tgtaattggc ttgtcaatgc taccctattg tttgccgtca tgaaagatgc
     1501 gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc aaaaaacttc
     1561 ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctactaggc gtaaacctga
     1621 aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc cgcagttcac
     1681 cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg tttatgcagc
     1741 aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg gcgcgttgca
     1801 ggcgcgttta acccagccgg gcaaacgcac cgggaaaatc tattatgtgc aggtggaagg
     1861 tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc
     1921 taccctgccc gccggcgcgg agctggttga cgaaccagcg tggttatggc cgcggaatcc
     1981 accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct tatatgaagg
     2041 acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc tgcgactgat
     2101 tcgttatgcg atgggtgatt acactttgga taaccttgcc aatggcgaat ggcgagaagc
     2161 aacagaataa ggatgcacaa tgtttaaacc gcacgttacc gttgcttgcg tagtgcacgc
     2221 agagggtaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat ggaaccaacc
     2281 tgccgggcat ctggaagccg atgaaacctt agtggaagct gccgcccgtg agctatggga
     2341 agaaaccggc atcagcgcgc aaccgcaaca ctttattcgt atgcatcagt ggattgcgcc
     2401 agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa tatgcccgac
     2461 ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag aaattttaaa
     2521 ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc aaagtgggca
     2581 acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg gtgtcatcta
     2641 aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga tccaatgtct
     2701 gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc
     2761 tggctgttgc aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa ctgggaagaa
     2821 gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca ggctgtctgc
     2881 gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg ggacaatgtc
     2941 ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga tattctgtgc
     3001 aacaaagaga tcaaatttaa agctttcctc gaatttgccg ccgaagattt aggtgccgat
     3061 tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag ccgcctgctg
     3121 cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag ccatgagcag
     3181 attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg taagattgct
     3241 gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg cttcattggc
     3301 gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg ggcaaccggg caaaatcatt
     3361 accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac tctcggtcag
     3421 cgtaaaggtc tgggtatcgg tggcaccaaa gaaggcaccg aagaaccttg gtatgtggtg
     3481 gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca cccgcggctg
     3541 atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc attcaccggc
     3601 actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg caccgtcaag
     3661 gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc cgtgacgccg
     3721 ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat tattgagcag
     3781 cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca gtgaacgtgg
     3841 caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg gcacgcctgg
     3901 tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc tcactcaaca
     3961 gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc gaagccaacc
     4021 tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc cagggcttaa
     4081 acgccgaatt aacccgctac acactcagct tgatggtgct tgagcgcaaa ctctcctcag
     4141 cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac
     4201 acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat gttgatgtga
     4261 tcagcccgct tggcccacgc attcaggtca ccggttcccc tgctgtactg caaagcccac
     4321 aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc
     4381 accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg accactcagg
     4441 caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta tcctcactga
     4501 ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg cgcgggattt
     4561 tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg ctgcaaaaac
     4621 tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca atcggttacc
     4681 ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa accatcgagc
     4741 gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa gtggcggaga
     4801 tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg gaagatatca
     4861 ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact
     4921 ggcgtcaact gattgatggc attaaagatc tcgccgttca gtaccgcgat atcccgctgc
     4981 tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag atggcaaacg
     5041 tcgcctaccg tatggagcgc cagtaccgcc agcttaacca ggtggagatc ctcggcaaaa
     5101 tcaacggcgc ggtcggtaac tataacgccc acatcgccgc ttacccggaa gttgactggc
     5161 atcagttcag cgaagagttc gtcacctcgc tgggtattca atggaacccg tacactaccc
     5221 agattgaacc gcacgactac atcgccgagc tgtttgattg cgttgcacgc ttcaacacca
     5281 ttctgatcga ctttgatcgt gacgtctggg gttatatcgc ccttaaccac ttcaaacaga
     5341 aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac ccgatcgact
     5401 tcgagaactc cgaagggaat ctgggccttt ccaacgcggt attacagcat ctggcaagca
     5461 aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg
     5521 gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac
     5581 tggaagtgaa ccgtgaccat ctgctggatg agctggatca caactgggaa gtgctggctg
     5641 agccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag
     5701 agctgactcg tggtaagcgc gttgacgccg aaggcatgaa gcagtttatc gacggtctgg
     5761 cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggcaaac tacattggtc
     5821 gcgccatcac catggttgat gagctgaaat aaactcgtat cagtgccgga tggcgatgct
     5881 gtccggcctg cttattaaga ttatccgctt tttatttttt cactttacct cccctccccg
     5941 ctggtttatt taatgtttac ccccataacc acataatcgc gttacactat tttaataatt
     6001 aagacaggga gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt tgttacgtca
     6061 ccaccttaaa gttcagattc aggatgctgg tcatcaggtc gatgatgcag aagatgccaa
     6121 agaagccgat tattatctca atgaacattt accggatatt gcgattgtcg atctcggatt
     6181 gccagacgag gacggtctgt cactgattcg ccgctggcgt agcaatgatg tttcactgcc
     6241 gattctggta ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat taagtgctgg
     6301 tgctgatgat tacgtgacta aaccatttca tattgaagag gtgatggcgc gaatgcaggc
     6361 attaatgcgg cgtaatagcg gtctggcttc acaggtcatt tcgctaccac cgtttcaggt
     6421 tgatctctct cgccgtgaat tatctattaa tgacgaagtg atcaaactga ccgcgttcga
     6481 atacaccatt atggaaacgt tgatacgcaa taatggcaaa gtggtgagca aagattcgtt
     6541 aatgctccaa ctctatccgg atgcggagct gcgggaaagc cataccattg atgtactgat
     6601 gggacgtctg cgcaaaaaaa ttcaggcaca atatccgcaa gaagtgatta ctaccgttcg
     6661 cggccagggc tatttgttcg aattgcgctg atgaaaaaat tactgcgtct ttttttcccg
     6721 ctctcgctgc gggtacgttt tctgttggca acggctgcgg tagtactggt gctttcgctt
     6781 gcctacggaa tggtcgcgct gatcggttat agcgtcagtt tcgataaaac tacgtttcgg
     6841 ctgttacgtg gcgagagcaa tctgttctat acccttgcga agtgggaaaa caataagttg
     6901 catgtcgagt tacccgaaaa tatcgacaag caaagcccca ccatgacgct aatttatgat
     6961 gagaacgggc agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa gatgatccag
     7021 cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa cgataccagc
     7081 ctcttgctga gtggagatca ttcgatacag caacagttgc aggaagtgcg ggaagatgat
     7141 gacgacgcgg agatgaccca ctcggtggca gtaaacgtct acccggcaac atcgcggatg
     7201 ccaaaattaa ccattgtggt ggtggatacc attccggtgg agctaaaaag ttcctatatg
     7261 gtctggagct ggtttatcta tgtgctctca gccaatctgc tgttagtgat cccgctgctg
     7321 tgggtcgccg cctggtggag tttacgcccc atcgaagccc tggcaaaaga agtccgcgaa
     7381 ctggaagaac ataaccgcga attgctcaat ccagccacaa cgcgagaact gaccagtctg
     7441 gtacgaaacc tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa ataccgtacg
     7501 acgctcaccg acctgaccca tagtctgaaa acgccactgg cggtgctgca aagtacgctg
     7561 cgttctctgc gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat gctggagcaa
     7621 atcagccgca tttcacagca aattggctac tacctgcatc gtgccagtat gcgcggcggg
     7681 acattgctca gccgcgagct gcatccggtc gccccactgc tggacaatct cacctcagcg
     7741 ctgaacaaag tgtatcaacg caaaggggtc aatatctctc tcgatatttc gccagagatc
     7801 agctttgtcg gtgagcagaa cgattttgtc gaggtgatgg gcaacgtgct ggataatgcc
     7861 tgtaaatatt gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga gcatctctat
     7921 attgtggtcg aggatgatgg ccccggtatt ccattaagca agcgagaggt cattttcgac
     7981 cgtggtcaac gggttgatac tttacgccct gggcaaggtg tagggctggc ggtagcccgc
     8041 gaaatcaccg agcaatatga gggtaaaatc gtcgccggag agagcatgct gggcggtgcg
     8101 cggatggagg tgatttttgg tcgccagcat tctgcgccga aagatgaata aatatgtcca
     8161 tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag cctgtggatg
     8221 cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg tcactggcag
     8281 aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat ctctccagac
     8341 gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag tcaccaggat
     8401 ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg tgaaaccaac
     8461 tggtcattac tggtacaggc agtgaaccac tggcatgagc cgaccgccgc gctgatgcga
     8521 ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatttcttt ttctgtacct
     8581 ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca gggtaccgga
     8641 cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc acatccggat
     8701 ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc tggtgatatt
     8761 ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc gatgaactat
     8821 tccgtgggct ttcgcgcgcc aaatacgcgg gaactgatta gtggatttgc cgattatgtg
     8881 ctgcaacgtg aactgggcgg caactactac agcgatcctg atgttccacc tcgcgctcat
     8941 cctgcggatg ttctgcctca agagatggat aaactgcgtg agatgatgct cgaattgatc
     9001 aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc acgtcatgaa
     9061 ctggatatcg cgccgccaga accgccttat cagccagatg aaatctacga tgcgctgaaa
     9121 caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg cgacgacgtg
     9181 tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc actcgccagc
     9241 aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc attcctcgcg
     9301 atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt ttggatttaa
     9361 gccggatgcg gcatcacatg ccgcatccct tttcgcttac ttccgttgcg ccgttaactc
     9421 ggcaatacgg acgatcacct gcaccgcttt ttccatacct tccagagtca caaactcatg
     9481 cttaccatga tagttgtaac cgccagtgaa caagttcggg cacggtaatc ccataaacga
     9541 caactgcgcg ccgtcggtac caccgcggat cggtttcagt tccggttcaa tatcgcagtc
     9601 acgcatcgcc tgctgggcga tatcgagaat atgcggatgc tcaaccactt tctctcgcat
     9661 attgtagtaa ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta accctttgcc
     9721 cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt tacggtcgaa
     9781 atcacggatg atgtagtgca tatcggcccg ttcaacggtg cctttcatgc tcgccaggtg
     9841 atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa cttccgcatg
     9901 aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc ccggatgaac
     9961 attgttaccg acaattttga tattgaccga tgcggcgttg aagttttcaa actccagttc
    10021 gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa
    10081 atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg
    10141 cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa tacctgcttt
    10201 gtcatcggca cctaacaagg ttttaccatc ggtggtaatc aacgtctgac ccagtagctg
    10261 atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca gcgcaatatc
    10321 gccaccgcga tagttttcaa caatttgcgg attaacattt ttgccgctgc aatccggtga
    10381 ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt tagccggtaa
    10441 cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca tttcttcgag
    10501 ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa cctgtctcac
    10561 ccctgctttt gattgggtat ccagagatac gtagttcaaa aatcgctcaa gtagtttatc
    10621 catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa gacaaatatt
    10681 gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc ttaatgaata
    10741 taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg ccgtagaatt
    10801 accgccctca ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc
    10861 cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg
    10921 ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat tccccagctg
    10981 gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa
    11041 acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg catcatgctg
    11101 gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac tgttttccaa
    11161 agctacgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg
    11221 caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa
    11281 ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc
    11341 gccattgctc gcgcggtggt taacaagcct cgtctgttgt tgctggatga gtcgctctca
    11401 gcgctggatt acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag
    11461 cttggtatta ccttcgtctt tgtcactcac gatcaggaag aagcgctcac catgtcagac
    11521 aggattgtgg tgatgcgcga gggtcgtatt gagcaagatg gtacaccgcg cgaaatttac
    11581 gaagagccga aaaacctgtt tgttgccggc ttcattggcg aaatcaatat gtttaacgcc
    11641 actgtcatcg aacgtctcga cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt
    11701 aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg
    11761 gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct gattggttac
    11821 gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc
    11881 aagatggtga tggtcagcga attcttcaat gaagacgacc ctgactttga ccactctctc
    11941 gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc tgatgaagaa
    12001 cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg tgttgtttgt
    12061 ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg acgccagttt
    12121 cgtcaaaatg gtctttacgc tggataacta cacgcgtctg ctcgatccgc tctattttga
    12181 agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc tggtgctggg
    12241 ctacccgttt gcctggtttc tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt
    12301 tctgctgatt gttcccttct ggaccaactc actgattcgc atctacgggc tgaaaatttt
    12361 cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc
    12421 aattcgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca ttctgctacc
    12481 gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagccgt tgcttgaggc
    12541 ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc cgtatcatta ttccactgac
    12601 gatgccggga attattgccg gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta
    12661 tgtatccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtcca
    12721 gttcctcaat attcgtgact ggccgtttgg tgcagccacc agcattacgc tgactatcgt
    12781 aatgggcctg atgttgctgg tttactggcg cgcttctcgt ctgctgaata aaaaggtgga
    12841 actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta cgcgtacctg
    12901 tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt tggcatcaac
    12961 tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag cctgttacag
    13021 gcagcgcagc attcactaac aatggcggtg ttttcggcga catttgctac gcttatcggt
    13081 tcactgacgg cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga
    13141 atgctgtttg tggtgatgat gtcaccagat atcgtgatgg cgatttctct gctggtgctg
    13201 tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca tatcaccttc
    13261 tgcctgccgt ttgtggtggt gacggtgtat tcgcgcctga aaggttttga cgtgcggatg
    13321 ctggaagcgg cgaaagatct cggtgccagt gaatttacca ttctgcgaaa aatcattctg
    13381 ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa gctttaccct gtcgatggac
    13441 gatgtggtag tttcttcgtt cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt
    13501 tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac catattactg
    13561 gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa aacgaaaggt
    13621 aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg
    13681 cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc tacaactgga
    13741 ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt attaaggcta
    13801 tctattcgac ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg
    13861 gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt aaagaaggga
    13921 tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat ccagacatgc
    13981 tcaacaagcc gtttgacccg aataacgact actccattcc gtatatctgg ggtgcgacgg
    14041 cgattggcgt taacggtgat gcggtggatc ctaaatctgt caccagttgg gcagatctgt
    14101 ggaagccaga gtacaaaggt agcctgctgc tgaccgacga tgcccgtgaa gtgttccaga
    14161 tggcgctgcg caaactgggc tactccggta acaccaccga tccgaaagag attgaagctg
    14221 catataacga gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc gataacccgg
    14281 ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggc tctgctttcg
    14341 ttgcacgcca ggcgggtacg ccaattgacg tggtgtggcc gaaagaaggc ggcattttct
    14401 ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga
    14461 tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc cgaaactatc ggttatccaa
    14521 cgccaaacct tgcggcgcgt aagctgttaa gcccggaagt ggcgaacgat aaaacgctct
    14581 acccggatgc tgaaaccatt aaaaatggtg aatggcagaa tgacgttggc gcagccagca
    14641 gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc aaacgggcgg
    14701 tggtattacc gcccgttttt tagttagcaa ggatgtggct aatgagtcag gacagtaaag
    14761 ttttatcccg ctctttcgtc ggtattgtgg tccccttgta ggcctgataa gcgtagcgca
    14821 tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca
    14881 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga
    14941 gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg
    15001 gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat
    15061 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
    15121 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
    15181 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
    15241 tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
    15301 cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
    15361 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
    15421 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
    15481 gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg
    15541 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
    15601 cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
    15661 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
    15721 catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag
    15781 gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
    15841 cgaccaggtc agagtcaacg atggtcagga tatttcccag acaaaccgct aataaatcca
    15901 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
    15961 cgggagcctg caacggttga tgataatagt gttgccagag ccacgcaaag ccccgacctg
    16021 atagataatt ttcgatacaa ccaatttgac cacagccgca gcggcgtaac gggaaatcca
    16081 gccccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
    16141 agcttttacc ggtaatcggt ttaccgttga aaatcagccc gccgccaacg ccggtgccga
    16201 gaattaaccc catcaccagt ggatattgcg taaattcatc atcccaggct tctgaaaggg
    16261 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
    16321 cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg
    16381 tttccggcat tcccggaata ccgatgccaa cagaaccttt acagccaaaa cgttggtcag
    16441 cttcggctac cagctcgcat actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
    16501 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
    16561 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
    16621 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    16681 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    16741 tccgatggca ggaagtcaat aaaatagata tcgctggaga ggaactgatg accgatcagc
    16801 ttttcaatcc gctcaataat cggggtaagt tgcagtgaaa caactacgcc gataatcaca
    16861 ccacacaggc taccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    16921 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    16981 gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    17041 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    17101 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    17161 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    17221 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    17281 tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    17341 ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag cgcatccgcc
    17401 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca ggcatccccc
    17461 tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc
    17521 tgaattgcgc gcagattggt accactttct accagtccgg tgaaattgat atacggcgca
    17581 gcggcggcaa tacccggcac tttttgcacg ttatccagtg cttcctgcca gttagtccat
    17641 ggctgattta ccgcctcaat ttcaccgtgc ggtaccacag caagaatgcg gttattcagt
    17701 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    17761 atgccaatgg tagaaatgac agagattagc gacaccatgc cgccgcgtcg ccgtccgcga
    17821 ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    17881 tcagttccgc cgtcagacgt ccatcacgca tttccagttg gcggctcata cgtttcgcca
    17941 gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa
    18001 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggc tcatccgcaa
    18061 gtaccagacg cggattattg accagcgcac gggcaatcgc cacacgctgg cgttcgccgc
    18121 cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct tttaacatct
    18181 ccagcgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagt ggcatagcca
    18241 cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    18301 tctggttgcg cagttctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa
    18361 agatcacatc gccggaggtt ggtgtatcca gcccgcccag caggtgcagc aaggtacttt
    18421 taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgccgacg ctgaaactga
    18481 cattgtgcag tacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    18541 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgagtggc ggcagcgcgc
    18601 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    18661 acctgtaaag gttcgatagc gaccggcagc gccgcgccat caagcaggac gccgattatc
    18721 ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca
    18781 ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    18841 aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccattaccat cagccctagt
    18901 gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagcaa gcccatcata
    18961 tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca gtcctgccat
    19021 ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg ctcatccagc
    19081 cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca
    19141 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    19201 ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc agatggtacc
    19261 atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg
    19321 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggcgta
    19381 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    19441 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    19501 actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    19561 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    19621 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    19681 aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    19741 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    19801 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    19861 gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct
    19921 gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc cgcaggatca
    19981 accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
    20041 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
    20101 atccgcatat gaccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct
    20161 atttcaatct ttaccttgca ccctttcgta tgccggtttt tttctttata tccggctatt
    20221 tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cggatctgga
    20281 acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta agtgcgctga
    20341 accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct tatgccgatt
    20401 ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg tatctgtatg
    20461 cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg
    20521 ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga
    20581 acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc gcaacaataa
    20641 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg
    20701 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
    20761 tggtgatcat gaagctgttt tatcagtacg agcaacgatt cggtatgcgc tccaccagcc
    20821 tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg
    20881 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg
    20941 aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact gttgcgggct
    21001 tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc ccgccttcac
    21061 tgcccgctgc cgtcagttac tctcgttaaa ccatcgcccc agccggggcg attcaatttg
    21121 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
    21181 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttataca
    21241 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca
    21301 acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    21361 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca gatggtgatg
    21421 aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc
    21481 tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt actgattgtt
    21541 ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg
    21601 gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact ggacagcgcc
    21661 ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg cggcgcgttg
    21721 ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    21781 atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg
    21841 atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    21901 agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg
    21961 agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt cagcgaacca
    22021 ctgccgccgc tgttcagtta tttccctgcc aataccttgc tcgtgaatac tggcgatctg
    22081 gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc
    22141 gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc
    22201 tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc
    22261 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa
    22321 gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg
    22381 gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct
    22441 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    22501 gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat
    22561 ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat
    22621 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    22681 ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac tggcgagtat
    22741 ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    22801 attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat
    22861 gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    22921 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag
    22981 cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    23041 attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    23101 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac
    23161 cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca ttacgacaac
    23221 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    23281 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc
    23341 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    23401 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac
    23461 gtggatattc tgacgcttac tgcaacgccg atcccacgca cgctgaatat ggcaatgagc
    23521 ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    23581 tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    23641 ggggggcagg tttattatct ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg
    23701 ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat gcgcgagcgc
    23761 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtaca
    23821 accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgcgcg
    23881 gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcgcatcat
    23941 caggcatatg catggttgct gacaccgcat ccaaaagcga tgactaccga tgcacaaaaa
    24001 cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct ggcaacgcac
    24061 gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    24121 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc
    24181 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    24241 ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc gttctataaa
    24301 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    24361 cgcttcggcc tgctgccaga tccggcgcgt accctgctgg atattgcccg tctgcgccag
    24421 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa
    24481 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    24541 cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg
    24601 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    24661 taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac
    24721 ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat
    24781 aaccttatat ttattattga tttttggtga tgatcaaaac gcgtttttct cgctggctaa
    24841 cgttttttac gttcgccgct gccgtggcgc taccggcaaa agccaacacc tagccgctgc
    24901 cgccagcgag cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa aatgacggtg
    24961 gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg ttacaggcta
    25021 accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa
    25081 ccctacttcc agacgcgccg cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct
    25141 attactaccc gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt ggtcagttag
    25201 gtggtgacac gctgacaccg acaatggtga ccaccgtttc agacaaacgt gcaaacccaa
    25261 cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt gagttgccta
    25321 cggtagtgcc ggctggaccg gataacccaa tgggccatca tgcgattcgt ctggcggcct
    25381 atggcggcgt ttatttgctt cacggtacga acgccgattt cggcattggc atgcgggtaa
    25441 gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc caggtcaccc
    25501 caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa ccaaacggtg
    25561 cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat ccgcagctgc
    25621 tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa actgacgctg
    25681 aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc
    25741 aagtgagccc acaaactctg taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc
    25801 gcggggaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt tataaattac
    25861 tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag attttgcgcc
    25921 catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg tccccgcgtt
    25981 agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt cgacagccgc
    26041 aaagttggca aatgacatgg agcttaaaat cgccgcagcg atgagggttt ttacgttttt
    26101 cataatagtg gccttatgca gatgaatgac gtcgcaagaa attacttaac gatcgataac
    26161 taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat cattaattaa
    26221 tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggg atctggactg
    26281 gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag gttcaacggc
    26341 atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc
    26401 gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg
    26461 acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa
    26521 tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc
    26581 gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac
    26641 tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg
    26701 atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca
    26761 cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg
    26821 gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag
    26881 gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga
    26941 acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct
    27001 gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca
    27061 gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc
    27121 cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat
    27181 acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct
    27241 ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac
    27301 cacgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc
    27361 cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact
    27421 cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt
    27481 gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga
    27541 caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt
    27601 tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg
    27661 gaatggctgg aaatacttaa ggattcggag aagtctgaag gaatgcctga tgcgacgctt
    27721 aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg
    27781 ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta
    27841 atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc
    27901 attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg
    27961 cgcgtcaggt tacccatcag gctaccgaca gtggagaagt tcgacagcga taccagcgaa
    28021 ccatgatctt tatactgata atttttcagc ggtttaccgt tcatctgcgc cagaatattg
    28081 ttcattgcgc aggtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct
    28141 tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt
    28201 tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc accgatatct
    28261 ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat
    28321 tcaccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc
    28381 acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat
    28441 gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg
    28501 cctttgtaac cgtagctgtg cagttgcttg accgcattgt gcaattcagc ggagagttct
    28561 acacccgtcg cgccgccgcc gacaatcgca atgttcactt taccattcgc gcccaggttg
    28621 gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg
    28681 ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta
    28741 cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct
    28801 ttctcatcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg
    28861 gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg
    28921 acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca cagatggctg
    28981 tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc
    29041 tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc
    29101 gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt tataaacata
    29161 gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg
    29221 aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg
    29281 cgactttacc gggtgagatc aaaaaacaga gtttattgtt tattatttga gcaagtccgg
    29341 aagggtgata tagggtgacg cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca
    29401 gcatcgcatc aggcattgat gctcgactgt cggatgcggc gtaaacgcct tatccgactt
    29461 acagaattcg gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc
    29521 cgagggtttt gaacgcctta atgcgctgca aatgcgggga gatattcttg aatttgtgcg
    29581 tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc
    29641 tgttaagcgc ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc
    29701 ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc
    29761 cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa
    29821 gaaaaccaat ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc agcggacgct
    29881 cgtcgacgtt cagttgcaac attttgtcca cttctttgag cagatgctgc atatcatgtt
    29941 tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta
    30001 agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg
    30061 cttcctcaca taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac
    30121 tgattcagac gggtgctgat cgccttccag cgagccgagt ccatcagttc ctgtcgcgaa
    30181 aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct
    30241 aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca acctgcgtcc
    30301 agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa
    30361 tcgtcagaga aaattacacc gtcaaaacac agttcctgac gcaaaacggt tttcagccag
    30421 taggaagaac cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata
    30481 atggcgtcga gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga
    30541 atctccgctt gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca
    30601 ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata
    30661 aaccggctgg caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc
    30721 gccgcgctga tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg
    30781 atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt
    30841 cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc
    30901 tgcacgcgtc caccttcctg atctaccgcc accaccagat gattgcgcga tgctgcgcgg
    30961 atctggcgca ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc
    31021 agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct
    31081 tcgacatcca acattactgg acccacactg ctctccttat tgttttatta atagctgccg
    31141 ccaggtgtca tcggccagcc tgataaattg ttgatcgccg atttgtcgcc agcggtactc
    31201 aaaccaccct gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa
    31261 ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc
    31321 agtattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc
    31381 ccagtcgatg agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat
    31441 atgcaatgga cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat
    31501 tcgcagccaa cccacagtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa
    31561 gggcaacagc gttattcgcc agccaaaacg tggttgttga tgaagataat acagcaagcc
    31621 tgccagttcg ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac
    31681 catccagtca cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa
    31741 agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac
    31801 aacaaaacgc tggccctgat gttcaatgag aaaactaccg ccactaagcc cactattaaa
    31861 cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg
    31921 attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca
    31981 tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag tacagcacgt
    32041 aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtacct aaactgtcct
    32101 gcggcgacaa acctaactgt tgcttagcca tcgacagctg ctgggcggaa accagggtaa
    32161 atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg
    32221 aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt
    32281 cagccccaag catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa
    32341 tatgcggcgc aggcggtgcc gtttgatctt cgtgctcaat tgggcctggc tgctgcggga
    32401 tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg
    32461 ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca
    32521 tcgccagcgc ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac
    32581 aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt
    32641 accgccgaat gtgcgggaat gctaacgctg cgtggccttt ccagaggatg catctccagc
    32701 cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta
    32761 taaagcgttg aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca
    32821 ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga
    32881 atttccggat gtgaacgaca gcccaccagc agtaacaacg ccagagacac cagcccaaag
    32941 catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac
    33001 ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga
    33061 tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga
    33121 tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc ggaatgagga
    33181 tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta
    33241 ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata
    33301 tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg
    33361 ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact
    33421 cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc
    33481 tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg
    33541 catatttctc atttgcattt acaaacaaaa ttattcgcac actaaatata accgctcatc
    33601 accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac
    33661 acaatttaac agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg
    33721 catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt
    33781 gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc
    33841 tacctctgcg ggtaccaaaa tgcagatgac tcaacttgat attcctcagt cggtcactat
    33901 tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa
    33961 cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgtgg
    34021 attccagatc gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa
    34081 tctgggcgac gcactttctg atatggcact ctttgaacgc gtagaagtag tgcgtggcgc
    34141 gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca
    34201 cgcgaccagt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga
    34261 acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat
    34321 tgtcggcggc taccagaata acgactcatg gctggaccgc tacaacagtg aaaagacctt
    34381 cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga
    34441 atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga
    34501 tggcagcagc aacagttacg atcgtgcacg cagtaccgca cctgactggg cgtacaacga
    34561 taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc
    34621 gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc
    34681 ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt
    34741 tgattatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt
    34801 gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctagtgt ttggtggcag
    34861 ttacagcaaa caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat
    34921 tggcagtttc tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct
    34981 ggcacaggac gataccacac atatgaaatc gttatatgct gctactcgag tcacccttgc
    35041 cgatccgctg catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac
    35101 ttacagcatg gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga
    35161 caactggtcg acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag
    35221 ttcaggcaaa tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga
    35281 ctggatgaat agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt
    35341 cgctcagtcc accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt
    35401 ggatgggaca gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg
    35461 gcagctgaca tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa
    35521 tcctaatctg ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc
    35581 agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac
    35641 accgtatggc accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg
    35701 ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac
    35761 ctacgatacc aacatggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac
    35821 cggcacgtat caattctgaa taacacctgt aaaaaaggca gccatctggc ggccttagtc
    35881 tccccaacgt cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt
    35941 atcggattta gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc
    36001 gcccagtttc ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag
    36061 acgggtaata catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag
    36121 agccggtgcc atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc
    36181 cggcgttttc agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc
    36241 ataaccgata ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat
    36301 aaagtcgatc agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat
    36361 tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat
    36421 gaaggagaac tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat
    36481 cataataccg cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc
    36541 ggcagctggc agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg
    36601 gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta
    36661 ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggcaccag
    36721 gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca
    36781 ctgagagaag gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc
    36841 agtaaagatg gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc
    36901 aagatactca ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc
    36961 ggagataatc cctccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc
    37021 aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc
    37081 aacaactgcg gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat
    37141 cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga
    37201 tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat
    37261 aggcagtacg gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc
    37321 attcttaaac ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg
    37381 tggcaagggg ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac
    37441 agagtaaaat aattcagtgc cgatttgttt gacggctatc acgtttcaac ttcagatcat
    37501 tggagaagtt gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac
    37561 cgcccaattt tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa
    37621 actcattcaa cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt
    37681 atcggtggtt acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc
    37741 tgcaacgtca cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag
    37801 gtaaggtgag tcagtttcca ccagtaaccg atccaggggg acataacgcg cagcatctcg
    37861 aagttgctcc gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc
    37921 cagtaattta cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc
    37981 cgtcactttt tcttcgcgca gaattgccag cgtatcggcg cgggcgtcac gggtatggac
    38041 gataaccggc ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg
    38101 acgaacttta gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac
    38161 accctcttct gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag
    38221 tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg
    38281 taaataaccc ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc
    38341 tttcgccaga acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg
    38401 gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa
    38461 cgcccggttg caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt
    38521 tgatgcctgt gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct
    38581 gcaggcgcga gggagaaaga tggtttgcca gttcgacgac aaggcccggc acatcaacat
    38641 tggtcacctg cgcagcacca tgatggcgtt ttagcgcatc catcagcaac gttgccagcc
    38701 agtgtaaacg cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc
    38761 ccgatggcac gctatatgcc aatgcctgac acaatgtttc acgagcctgc cagttatctc
    38821 cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta
    38881 atgcatcctg tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg
    38941 gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct
    39001 cgcgggtagc caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg
    39061 cgttagccgc ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac
    39121 ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg tactgcatca atgcccagcg
    39181 tatttttccc tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca
    39241 actgacatcc acgacagtga ccgcaacttt tgtggccctg cggttgttgg cagagtaaat
    39301 aacggctcag ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta
    39361 gcgcatggtg acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta
    39421 accatggata ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat
    39481 tgcatccatc acggcctcca gcggctgggt ggcatcaatg gtatgaatac ttttatcttg
    39541 tgcagccagt tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc
    39601 aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc
    39661 gagatagagc gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag
    39721 catatgttgg tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg
    39781 atcgccaatc acccaggtgc cgttagccag agctggtttg atgaccgttt ctaccagttg
    39841 aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac
    39901 cgatttgata tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc
    39961 ccgagtgaaa accatgtcgc ggatacccag ttgctcaagc gtctcaacca ccacattacg
    40021 cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt
    40081 tttccttaag cactttcaga taatcctgca cagacttgtt atggctggca agattggtat
    40141 taaacgtgtg accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat
    40201 gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg
    40261 taatggtata ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat
    40321 tataacgctc tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc
    40381 gtaaacggtt gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt
    40441 tttcgataat tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg
    40501 cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta
    40561 acgcgacatc ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa
    40621 tccactccgg gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca
    40681 gcgtgtgctt gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc
    40741 cttctaccag tcgcagcggg aactgtgctt ctttaccgct ttccagcaat ttcagcatct
    40801 cgcgcacggt catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg
    40861 gttcgatacg cagcaaccat tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa
    40921 gctgttcacc aagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt
    40981 tgataagcaa tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga
    41041 tacccagtac caccagcaat aacaagataa tcaataacac ttttttcatg actaattcgg
    41101 gcgctcacaa agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc
    41161 acaggcacgt acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg
    41221 caacgcctct tccagagagg cttgcacttc gacaagctga taagaggatt gtgccagcaa
    41281 acggatacag aattgtcgca taatgccgtt aacacctgcc tgatccagac gcggcgtata
    41341 aactacattg cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc
    41401 gctgtcaagg accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac
    41461 ttgctcaagc cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac
    41521 cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac
    41581 cgtaacggag agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc
    41641 actaccgcga ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt
    41701 catctcttgt tcaagctgag gccagaagtc acagaaaatc atcaaccgct gacaagcatc
    41761 ctgtagtcgc tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt
    41821 ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt
    41881 atgaccgtta attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg
    41941 ttcactacgg aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta
    42001 tatgggtggg aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg
    42061 aagccgaagg agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt
    42121 acgaaatcca gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc
    42181 tgatcgcgca gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg
    42241 tgaccggtca tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg
    42301 gttttcaccg cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta
    42361 acgtagccaa tctgactcgc ttcaatgcct gcatcacgca gagcatttgc catcgccaga
    42421 gctgcgcctg cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca
    42481 aagccgacga gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca
    42541 agtaccagca taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg
    42601 cggctcgccg cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca
    42661 ccaacaccca gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg
    42721 ccatacgcga taatacgcgc agcatggcca atgttgtgca cgccggaagt acaggcggtc
    42781 gcgatagaga tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg
    42841 ttcacaatcg ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga
    42901 gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca
    42961 atgcgggttg cgttctcttc cgttatatca aggccagaat cctgcatggc ctgaacgcca
    43021 gcgacaattc catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg
    43081 tcctcacagt taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg
    43141 aaatggtcga ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct
    43201 acggtattgc cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac
    43261 acgtttgtcc tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg
    43321 cggtcgaacg accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg
    43381 gcagcctgaa cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct
    43441 tcttccagag ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg
    43501 aaagaagcat tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc
    43561 ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg
    43621 atggttttcg tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac
    43681 cacgatttta cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat
    43741 tattactcat aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag
    43801 accatgtaca tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg
    43861 gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga
    43921 acctgcgcca ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca
    43981 ataaagcccg gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt
    44041 gatttactga agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt
    44101 cccatggtac caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata
    44161 gcgcgcatta ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg
    44221 ttccactctt catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg
    44281 accaggatat ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat
    44341 gccgggtcgg tcacattcaa catcagacct ttgccgttgg cacctaaata atcgctgatc
    44401 gcctgagcgc cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc
    44461 gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct
    44521 tcaaaattca tgattttcct cttttaaagc tcaagcgccg ctgccatcgc tgaaggttcg
    44581 ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg
    44641 cccgggccga cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc
    44701 gtccactgaa ccgggttata caactgacgt accagtgcgt cacggatggc atcaccattg
    44761 gtttcgcatt tcacatcaac gttattcaca acagaaactg ttggtgcgtt aaaggtgatt
    44821 ttcgctaatt ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt
    44881 acgctcactg gtaacggcag agcacgtttt gcgcccgcag ctttacaggc agcgccagca
    44941 cgctcaaccg cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc
    45001 ggagaaacga cctgacctgc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc
    45061 agaccgatga ttgccgccat agcgcccgta ccttccggta cggcttcttg catgagcttg
    45121 ccgcgcatct caaccagacg caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc
    45181 gcggaatatt cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc
    45241 tgccatacgc ggtacagcgc aacagaggca gtcaacagcg ctggctgggt ttgccaggtt
    45301 ttattcagtt cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc
    45361 gcagaagctt cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc
    45421 attccaacgg tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa
    45481 tccttatcct agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca
    45541 agcagaacca actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac
    45601 gggacagagg ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccata
    45661 gacataccga gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc
    45721 agccagtcca gttgagaacg gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc
    45781 gccagttccg ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa
    45841 ttctctggat tcacacggtc agcattaggc agcgtcagca actcaccata gctaccgtcg
    45901 gcatgcagat gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc
    45961 gcgccatcgc caaaaataat aatagtccca cgatcggttg gatcgcaggt gcgcgccagt
    46021 acatcggaac cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct
    46081 acgcttaatg cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct
    46141 ttaatgccca gcatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa
    46201 gtcgttgcca caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg
    46261 cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt
    46321 tcgcggatac cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa
    46381 tcggcgtttg tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac
    46441 atgtacgctc agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc
    46501 agcgtcctgc taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg
    46561 gtatacagat tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac
    46621 tgcctgttca atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac
    46681 cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa
    46741 tcgcctcgtc aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt
    46801 tttcccttca ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt
    46861 ctttaatgtg acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa
    46921 caactcattg gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc
    46981 tgaggcatcc cgaatactgt cgagaccctt tacttcttct tcaccaatat tgagcaacgc
    47041 cacgcgagga ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa
    47101 ttgcaccaac attgtactgt cacaatcgac gttggcccct aagtcaagga ccaccgtttt
    47161 gcccttttgc tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg
    47221 cttgagtaat aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc
    47281 ttgcgctcga ccttctttca ccagctccag ggccacgcgc attgaactcc cacgactggc
    47341 gcggatagct tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg
    47401 cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt
    47461 gccgactaaa agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac
    47521 tgtcacggaa gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa
    47581 ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc
    47641 ttcatcacgc agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg
    47701 gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg
    47761 actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc
    47821 tgttgtacgg ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc
    47881 accaattact tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcacttct
    47941 tcaggcagtt caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc
    48001 accggaacta ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt
    48061 tcaccgaatt cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg
    48121 tctgaacgca caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg
    48181 caacgctgac actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt
    48241 tggttatcga tagcgaacga catggagcat tccacatcac tgtccacact gactacggat
    48301 tcggcgacgc gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga
    48361 gccgtacgaa ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat
    48421 taactttgta acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata
    48481 attcgcacac attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac
    48541 ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa
    48601 aaaatatgcc taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa
    48661 aactgcaaat ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg
    48721 aatcaccgcg acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac
    48781 gttatccgga tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca
    48841 ccggtaaacc gttaacggaa gaaaatgcct gtctgcaatt acgcaaagcc agcggcaata
    48901 tcgtcacctt ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag
    48961 aagtggagcc ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg
    49021 tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta
    49081 cgctgtttga gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg
    49141 cgctgtgtca gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc
    49201 tggatgcggt tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc
    49261 ttatcaggcc aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc
    49321 ttttgcaaac aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg
    49381 gtccccggat gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg
    49441 cctgcttcag tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc
    49501 ggatgacccg catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta
    49561 caacgcacca gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt
    49621 ttgccttcct gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac
    49681 ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct
    49741 ttttgcattc ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc
    49801 gctatcagca acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc
    49861 gcttccgggc gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca
    49921 tgtaccgccg taccggaagg tttattcagc accaggatat gatcatcttc atataagatg
    49981 acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca
    50041 gcaacgcgaa ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta
    50101 atacgttttt tgttcacccg cacttcgcct ttacgcaaaa tacggtaaat catacttttt
    50161 ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg
    50221 gtgatagcaa caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag
    50281 ccaacaggat tcgcgccact catttttcta tgcttatatt tagttttgca ccttattact
    50341 tcactacgtg atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa
    50401 ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg
    50461 aataatgagg ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc
    50521 attttgcccg accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag
    50581 ttagcgggct gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac
    50641 ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact
    50701 ctgcgcctct ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca
    50761 tcggttcaca cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa
    50821 tgagtaagtt acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt
    50881 tgcccttgta gatgggcagc gtctttatga cctggatatc gaaagtccag ggcacgagca
    50941 gaaaaaggca aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc
    51001 ttttgttgat tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga
    51061 atatttccct gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga
    51121 aggtcaggaa gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt
    51181 aaccaccttt atcagtctgg cgggtagcta tctggttctg atgccaaata acccgcgcgc
    51241 aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag aagcactggc
    51301 aagccttgaa ctgccggaag gcatggggct tatcgtgcgc accgctggcg taggcaaatc
    51361 tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa
    51421 agccgctgaa agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg
    51481 cgcattccgc gattacttac gtcaggacat cggcgaaatc cttatcgata acccgaaagt
    51541 gctcgaactg gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat
    51601 caaactgtat accggcgaga tcccgctgtt cagccactac cagattgagt cgcagatcga
    51661 gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt tcgattgtta tcgacagcac
    51721 cgaagcgtta acggccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga
    51781 agaaaccgcg tttaacacca acctcgaagc tgccgatgag attgctcgtc agctgcgcct
    51841 gcgtgacctc ggcggcctga ttgttatcga cttcatcgac atgacgcctg tacgccacca
    51901 gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat
    51961 cagccatatt tctcgctttg gcctgctgga aatgtcccgc cagcgcctga gcccatcact
    52021 gggtgaatcc agccatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa
    52081 cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac
    52141 ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg
    52201 ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa
    52261 cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc
    52321 gaccttaagc tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga
    52381 agagttcgct gaacgtaagc gtcctgaaca acctgcactg gcaacctttg ccatgccgga
    52441 tgtgccgcca gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa
    52501 agctgcaacg gcaacaccag cagctcctgc acaacctggg ctgttaagcc gcttcttcgg
    52561 cgcactgaaa gcgctgttca gcggtggtga agaagccaaa ccgaccgagc aaccaacacc
    52621 gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg
    52681 ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg
    52741 cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag actgccgaga cgcgtgagag
    52801 ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg
    52861 tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc
    52921 gctgaatgtt gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt
    52981 tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc
    53041 cgaagaagcg gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca
    53101 ggaagctcca gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac
    53161 tgcaccagaa cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg
    53221 tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg
    53281 tgacgagcgt tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga
    53341 actggcctct ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt
    53401 tgaagagcag cgcgaacagg aagaagtaca agtgcagccg atggtgactg gggtccctgt
    53461 cgccgccgct gtcgaacctg ttgttagcgc accggttgtt gaagaagtgg ctgaagttgt
    53521 agaagccccc gttcagattg ccgaaccgca accggaagtg gttgaaacga cgcatcctga
    53581 agtgattgcc gccgcagtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt
    53641 agcccaggaa gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag agacggcaga
    53701 tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag ttgttgctca
    53761 acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag ctgcggttga
    53821 acctgaggtc accgttgagc ataaccacgc tacggtgccg atgacgcgcg ctccggcacc
    53881 ggaatatgtt ccggatgcac cgcgtcacag tgactggcag cgccctactt ttgccttcga
    53941 aggtaaaggt gccgcaggtg gtcatacggc aacacatcat gcctctgccg ctcctgcgcg
    54001 tccgcaacct gttgagtaat aattagctca agtaatcaag ccctggtaac tgccagggct
    54061 tttttatttc atctttgaat caacatatta aaattcatcg aatggcatcc ttgctaacca
    54121 acaatgcaaa ataggcaaaa aaatgcccgc cccggcgatc ccagagcagg cagacaaaac
    54181 atacccagtt tcatgatatg ttttaaagcg aaattatttg ttgagctgga agagcgacaa
    54241 tccctgcata tcggtaaatg ctttatacga tgcctgcaat gccgtttgct gcatgatgta
    54301 agatgaaata gttgcattcc agtccacatc aaccagatcg ctcatctgct gcgtttgccc
    54361 taaagcgcga tcgctaccta atgaatccag cgactccagt tcgttcagct gcgtgcctaa
    54421 ttccgcgcga acggtcagca cattgttcag cgagtttttc agtccgcggt tggttttatc
    54481 taacgccgca gcggcggttt ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc
    54541 tgcgatggcg ctatccagca tggcaaaaag attggtttca gaagcgctac cgtctggttc
    54601 cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat
    54661 cgaacgcgaa gcatcgacct gttgcttaat actttctgtt ccgccgacat aatcaccgtt
    54721 agcctcacta aacggcgcag tctctgtttt ataaccggca aaaatgtagc gcccattacc
    54781 gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat ccgtagccag
    54841 cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt tttcctgagc
    54901 attctggata gcagtggtga cctggctaag tacactctct tccagtgaca ctttttgagt
    54961 ggcgaaagta cgcgccagcg tgtactggct gttttgcgcc tgtgcctggg agagaacaac
    55021 cgcttgtgat gcagcaatgg gatcatctga agggttaacg actcgcttac ccgtcgacat
    55081 ctgttcgccg tacttcatcc attctgcctg agaattggtg ataccacgca tgttttgctg
    55141 gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt aatcagcgca
    55201 tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa
    55261 cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg
    55321 gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt cgcggtttta
    55381 ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc accgcccacc
    55441 gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttatc gctatcaccc
    55501 gcatcttttt cgctcgccat cgctattttc gcttcgtcgg tgattaatac atccatgttg
    55561 acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg cgttcctgta
    55621 aacgtcaact ccagaccatc aaatgccact ttaccgttgg catccggcgt caccgtaaaa
    55681 gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga gattttgtaa
    55741 tctgtcgcca gtaccacgga ggcatcagtt accgtggcac cgatcgcaac gtcacctttg
    55801 tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa agaaatcttc accagcatcg
    55861 ccgttagcat caaatccggc tttgtgttgg gtgttgaaag cctcggcaaa tgccagcgcc
    55921 agttgtccaa gcgtattacg cgtctggtcc agctcctgag aacggaatgt cagaatgccg
    55981 cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc cgtcccatca
    56041 acataagcga cagtcgtacg agaagggtca gcgctggaag gaactgccgc cagttgccgc
    56101 gccgtacttc cctgaaccag tgagtaacca ttggccaccg tgatgttata agtgccgcca
    56161 tcctgaacgc tgacttctac accaacaatc tggtttaatt cgctcaccag ttgatcgcgt
    56221 tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg cgagatttga
    56281 tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc
    56341 gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt aaactgattc
    56401 accaaccctt ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc
    56461 accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg
    56521 gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcggtcag gccgctactt
    56581 tgcgtctgcg cagcacgtaa ctggttggta ataaacgcat catactcacg ctgcacacca
    56641 gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc
    56701 ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc
    56761 gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac
    56821 atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt
    56881 gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta
    56941 tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga tcggtagcat
    57001 accccgcgtc ctgtagggcc tgcgccccct gctccgcact cgcggctgtc gtcacggcgg
    57061 cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc
    57121 tgtagacccg aaatttcgct tttactttct tcgcttcgcc attttcatat tcagtcgtgg
    57181 tgatttcagt aactggacct ttccagttgc cagaggcttt gacgccaaac aggttatagc
    57241 tcggctcgcc gttttcgcgg cggatttgcc gttgccccca accagactcc agcgccgcct
    57301 gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg
    57361 agagttgcgc gaggaatgct ttactgtcac ccggcagcga atcatcgtag ttacgtggca
    57421 cggccttttg caccagctgc gaaagcgcct gattttgata acgcaccaca gtttcgagcg
    57481 ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct
    57541 gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaatct
    57601 gctggtcata catactggta tacaggcgag tgtgctcgct gctgaacagg ccatcttttg
    57661 gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg
    57721 ccaccggacg gatatttgcc gccggatctt cgcccgcttt cgcctttagt tcgttgagtg
    57781 attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc
    57841 cagttttgcc cgcagacatc ccgcactttg cattgattgc agtatggaca tcagatccat
    57901 cggcgtagcg cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg
    57961 tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac
    58021 agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattgactgt
    58081 tactgagaga tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac
    58141 caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg
    58201 catattctgg atatctgcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac
    58261 ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt
    58321 gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt
    58381 cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc
    58441 accgttcagt tggttcacct gaacactgct accgccagcg gaggctcctg cgccgccaac
    58501 cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggcgtcat
    58561 caacaacgta cctccacgca agcttttggc atttcccatg gaagaaacca ccacatcgat
    58621 ggtttgcccc tgacgtccaa acggaggaag tgactctgtc accattaccg cagcgacgtt
    58681 ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt
    58741 aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccggtgccat ccagccccac
    58801 caccagacca tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg
    58861 aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt
    58921 aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc
    58981 atattttgcg cttcgttaat gtagccattg cctacgtatt caatgcgcgc atccgccacc
    59041 tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac gccagagaag
    59101 cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg
    59161 ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca
    59221 ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg
    59281 ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg
    59341 tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg
    59401 gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag
    59461 ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc
    59521 ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt
    59581 agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct
    59641 atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttaagcctt agagttgcgt
    59701 cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg
    59761 ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata
    59821 cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtacaccaga
    59881 ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agcccggtgt cattcataaa
    59941 ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct
    60001 gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg
    60061 ctgcacctga aaaccaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta
    60121 taggctgatg aaccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca
    60181 tctttgctgt tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc
    60241 gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt
    60301 gcccccggct ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta
    60361 aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg
    60421 gcgtcaaggc ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat
    60481 tagtgacaaa gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta tccggcctac
    60541 aagtgctcgc aaaatcgata aattgcgatt gcccccatag acccgataaa cttgcgcatc
    60601 gggcaactta tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggca
    60661 cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg
    60721 ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc
    60781 gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct
    60841 gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc
    60901 agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag
    60961 attgtgccat cggcagcaat agtgatttcc gctccttccg gcacagcaat tggccccgcc
    61021 tcgcctatca cctgatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg
    61081 ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca gccatcctgc
    61141 tgcaacgcga cgtccagcgg gcgcgaggtg taatccattt tgccgggcgt catatctgcg
    61201 cccggcgttg acgccgtgac caacgtgcgc gtgggcagag aaagcccttc cactggcacc
    61261 gcgcgtaaag cattcaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg
    61321 gcggttaccg cctgttgatt cagtgtctgg ctggctgctc ccatcgcggt ataaattgcg
    61381 tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg
    61441 gtcctgagtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa cgatcatatt
    61501 gaccagttct ttactgagat cgacattgga cgcttccaac gcaccgttgg tcagggtgcc
    61561 aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca
    61621 gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg
    61681 ccccagcagt tgtgtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat
    61741 ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca caatattgtt
    61801 agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggatctgc
    61861 gccgttaatt gcgccagtgg taatattctc tgttggattt gaggtcagaa cgccattggc
    61921 attaaacacc agcttcattg caggctcggc tgtacctgtt ggatcactgc tatcctgggt
    61981 gtagacctgc cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt
    62041 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    62101 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    62161 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    62221 cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg
    62281 catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    62341 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    62401 tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    62461 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    62521 gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    62581 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    62641 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    62701 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    62761 ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    62821 gccatcagtc aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat
    62881 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    62941 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    63001 ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc
    63061 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    63121 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    63181 tggattggtc gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg
    63241 taagtctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    63301 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    63361 agggttttca gcatcatgct tttcaccatg ttgagcactt caacattggc ctgatagctg
    63421 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg
    63481 tagccctttg catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga
    63541 ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc
    63601 tggaatacca cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca
    63661 ttcgccagat tactggccgc cacgttcaag cgctgggact gggcagttaa cgccgacccg
    63721 gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc
    63781 atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg
    63841 gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc
    63901 ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc
    63961 gttgaggtca tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt
    64021 ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg
    64081 atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa
    64141 cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat
    64201 cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt
    64261 tatgccgttg atgaccattg cggacaggta caattcacgt tgtagaaatg gctgggggcg
    64321 aaaatgctgg caataaaacg tagcgtggcg atcatctcga tactattcac tccgttaagt
    64381 gcggcgagta atctcacatc gcaattgcaa acctttttta gcgcccagct cgcgggggta
    64441 agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc gtgcgagcag
    64501 ccattgcttt cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc
    64561 tgcggtaacg acaaacgata tttacaggtt aatgtacagg ccacaggaaa ttatgtggtt
    64621 gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actaaaacgc
    64681 ggacggcagg ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc
    64741 gttagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca
    64801 tggcgggtaa aagcgggaca acgcgtcaac gtgatcgcca gcggtaacgg gtttagcgcc
    64861 aacgcagaag gtcaggcgct gaacaatgca gccgtcacac agaatgcgcg ggtgcgcatg
    64921 gtatcgggac tggtggtcag cggcgttgtt gatgcagatg ggaatattct tataaaccag
    64981 taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc
    65041 ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc cgtaagcacc
    65101 gttcaaccac gcgaaaccac tgacgcgtcg gtaacgaaca cccgggcggc aaaaacaaca
    65161 gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc
    65221 agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa
    65281 ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg
    65341 cagagtaact gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct
    65401 taacgatctc aaaacggtaa tggatcaaga acagcaacat ctctctatgg ggcagatcaa
    65461 cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta
    65521 cctcgaacag ttacgcagga aagaacccaa tacagcaaat agcgttgata ttagtcaacg
    65581 ctggcaggaa attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg
    65641 gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca
    65701 ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg gcggtaaaaa
    65761 gatttcgatc tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg
    65821 tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg
    65881 caatccccgc cagcacgacc gccattaaac gcagtaagcg ccaaggcatg gtacccaatg
    65941 accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca
    66001 ccaccagacg caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac
    66061 gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca
    66121 gcccggcatg tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg
    66181 taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca
    66241 acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg
    66301 tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac
    66361 ccgaaagaat gcccaacgca accgcactcg gcagcgccaa caggaaacaa agacgcaacc
    66421 cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg
    66481 acggcagcaa aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc
    66541 ggtcggcgta atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt
    66601 tgatgattaa ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca
    66661 ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg caggaccagc atgccgatct
    66721 tcttcaggtg cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg
    66781 ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc
    66841 tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa
    66901 tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta
    66961 gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga
    67021 tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca
    67081 ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt
    67141 ccgccagaat cggtacaaat gccagggaaa atgccccttc ggcaaagata cggcgtaaca
    67201 agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc
    67261 tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga
    67321 cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac gggcaaaacg
    67381 cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg aattgctacc
    67441 gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt
    67501 tgcgccagca ctgcctgttc gccggcggtt tgcagaactg tctgattttg cacacattca
    67561 atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga
    67621 atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg
    67681 tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga
    67741 tgcatacagg tggtgatctg caaaggatcg gccgaaaaat ggtgctcggc aaacagcatt
    67801 tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac
    67861 cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt
    67921 ggcccgacac tattgctacg atgtttatcc attcttagcg aagctgcggt agcgagttgc
    67981 gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt
    68041 tttttccgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc tgccagcggt
    68101 ttatcgacac agacatgtac acccgcattg agtaacgtac tgaccacgtc aaagtggctg
    68161 gcggtgctgg aatgcacaaa aaccgcatcg caactggcgg caaggctgga taacgaatcg
    68221 gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag
    68281 gctccttgta acatccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca
    68341 atgccgccta atcccacaac gccgatacgt aattttttca ctttaatctc ccagatgggc
    68401 cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg cttcgacgcg
    68461 ggcctgtaaa tcaccatcaa ccgcgttcga catatccgtt accgccggct gatcctctac
    68521 ctcaccactg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg
    68581 caccacaaaa ggaccatctt cgcgatttgc cagttgttcc agcgtcgact ccacttccgc
    68641 catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc
    68701 accacgcaat aacaacgtgg tgattaacgc cacttccgct gcgctcagtt tcagatcgcc
    68761 aaattctgaa ttacaaaaac gttgttcata tttggtgacc cgattaccaa agccactcac
    68821 cgtgcgcaga taatggcgtt tgaccagatt atccagttgc tcctgcactt cagattcact
    68881 cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac
    68941 tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caatcacgcg
    69001 agcttcaagg gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt
    69061 ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt
    69121 ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat
    69181 tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa
    69241 tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat
    69301 aaccgagata gcaggcatga aaagagccac gaacaacatt ggaaaaattg gcaacgccaa
    69361 taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa
    69421 attcgttaat catccccagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc
    69481 gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc gcaagacgcc
    69541 aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg
    69601 gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac
    69661 gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt
    69721 ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat
    69781 attgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct
    69841 tttatttccc gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt
    69901 tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct
    69961 ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc
    70021 tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg
    70081 gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg
    70141 gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca
    70201 cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtt
    70261 gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt
    70321 tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt
    70381 ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac
    70441 gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg
    70501 tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta
    70561 tggtcaacga cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt
    70621 gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg
    70681 aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg
    70741 tcagcggtct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg
    70801 ccgagcctgc gcgtgaaacc ttaagtgctt tgctggcgga cgcaagagct cgcggcagct
    70861 atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg
    70921 gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg
    70981 gaattattgg catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgcaa
    71041 cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg
    71101 aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc
    71161 cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg
    71221 ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca
    71281 ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg
    71341 ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc
    71401 tgcgcaaggt caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat
    71461 ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg
    71521 cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg
    71581 atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg
    71641 aagatgatat tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact
    71701 ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt
    71761 tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc
    71821 cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag
    71881 gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca
    71941 ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa
    72001 caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc
    72061 ggcgtggctg acgcccatat tgttctgaca aacttgatca gccaaattgg tcgcgaagag
    72121 ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt
    72181 cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt
    72241 gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa
    72301 acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag
    72361 caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg
    72421 gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga
    72481 gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg
    72541 tccggcaaaa acattccttc agccggagca tagagattaa tgactgcacc atcccaggta
    72601 ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa
    72661 actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct
    72721 ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta
    72781 cctgccgggc acgatttcac cccattgata acctgttatt taacagattc gctggatcct
    72841 aatgagctgg agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca
    72901 aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt
    72961 gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat
    73021 atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag
    73081 cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat
    73141 gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac
    73201 cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa
    73261 cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc
    73321 ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg
    73381 ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat
    73441 gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta tgggttgccg
    73501 gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca
    73561 ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa
    73621 caataaaaat cgtagcttcc tgttgtcatt aggttattta acctgtataa ataaccagta
    73681 tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc
    73741 agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc
    73801 tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg
    73861 cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc
    73921 cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg
    73981 gcttttttat tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt
    74041 gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa
    74101 agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat
    74161 actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga
    74221 attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt
    74281 gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc
    74341 caaataagtc cgagcaggaa gggactgaag ttggtcaaac gctctggtta accactgatg
    74401 ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt
    74461 tccaggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct
    74521 caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa
    74581 accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg
    74641 taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg
    74701 cccatatgcc accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg
    74761 cttatggtga aggcgaaaag tatgtcccgc tggtcctccg cgcgcaaacg ctgtgggatg
    74821 aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg
    74881 gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca
    74941 acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg
    75001 acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta
    75061 aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca
    75121 ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg
    75181 cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagcagcctg
    75241 tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga
    75301 ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg
    75361 cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg
    75421 atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc ccgttcttgc
    75481 gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt
    75541 cgcctgacga agattttatt atcgataccc tacccggcca cgataataca ctgctcatta
    75601 ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt
    75661 ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat
    75721 aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct
    75781 gttgcactat ctca
//
LOCUS       DAZPLM010000002        65646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPLM010000002 DAZPLM010000000
VERSION     DAZPLM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.002"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..616
                     /locus_tag="ACHVC7_000076"
     CDS             <1..616
                     /locus_tag="ACHVC7_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2114237.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(613..2091)
                     /gene="scpC"
                     /locus_tag="ACHVC7_000077"
     CDS             complement(613..2091)
                     /gene="scpC"
                     /locus_tag="ACHVC7_000077"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR2114238.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="ACHVC7_000078"
     CDS             complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="ACHVC7_000078"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR2114239.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="ACHVC7_000079"
     CDS             complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="ACHVC7_000079"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR2114240.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="ACHVC7_000080"
     CDS             complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="ACHVC7_000080"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR2114241.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6246..7139)
                     /gene="argP"
                     /locus_tag="ACHVC7_000081"
     CDS             complement(6246..7139)
                     /gene="argP"
                     /locus_tag="ACHVC7_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR2114242.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7569..8228
                     /gene="rpiA"
                     /locus_tag="ACHVC7_000082"
     CDS             7569..8228
                     /gene="rpiA"
                     /locus_tag="ACHVC7_000082"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR2114243.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8484..9716
                     /gene="serA"
                     /locus_tag="ACHVC7_000083"
     CDS             8484..9716
                     /gene="serA"
                     /locus_tag="ACHVC7_000083"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR2114244.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9945..10004
                     /gene="ibsC"
                     /locus_tag="ACHVC7_000084"
     CDS             9945..10004
                     /gene="ibsC"
                     /locus_tag="ACHVC7_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR2114245.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10336..10395
                     /locus_tag="ACHVC7_000085"
     CDS             10336..10395
                     /locus_tag="ACHVC7_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2114246.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10496..11098)
                     /locus_tag="ACHVC7_000086"
     CDS             complement(10496..11098)
                     /locus_tag="ACHVC7_000086"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR2114247.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11119..11302)
                     /gene="ssrS"
                     /locus_tag="ACHVC7_000087"
     ncRNA           complement(11119..11302)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHVC7_000087"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="ACHVC7_000088"
     CDS             complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="ACHVC7_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR2114248.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11841..12419
                     /gene="ygfB"
                     /locus_tag="ACHVC7_000089"
     CDS             11841..12419
                     /gene="ygfB"
                     /locus_tag="ACHVC7_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR2114249.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12445..13770
                     /gene="pepP"
                     /locus_tag="ACHVC7_000090"
     CDS             12445..13770
                     /gene="pepP"
                     /locus_tag="ACHVC7_000090"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR2114250.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13767..14945
                     /gene="ubiH"
                     /locus_tag="ACHVC7_000091"
                     /gene_synonym="visB"
     CDS             13767..14945
                     /gene="ubiH"
                     /locus_tag="ACHVC7_000091"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR2114251.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14968..16170
                     /gene="ubiI"
                     /locus_tag="ACHVC7_000092"
                     /gene_synonym="visC"
     CDS             14968..16170
                     /gene="ubiI"
                     /locus_tag="ACHVC7_000092"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR2114252.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16244..16327)
                     /locus_tag="ACHVC7_000093"
     CDS             complement(16244..16327)
                     /locus_tag="ACHVC7_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114253.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16617..17711
                     /gene="gcvT"
                     /locus_tag="ACHVC7_000094"
     CDS             16617..17711
                     /gene="gcvT"
                     /locus_tag="ACHVC7_000094"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR2114254.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            17735..18124
                     /gene="gcvH"
                     /locus_tag="ACHVC7_000095"
     CDS             17735..18124
                     /gene="gcvH"
                     /locus_tag="ACHVC7_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR2114255.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18243..21116
                     /gene="gcvP"
                     /locus_tag="ACHVC7_000096"
     CDS             18243..21116
                     /gene="gcvP"
                     /locus_tag="ACHVC7_000096"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR2114256.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21382..22125
                     /locus_tag="ACHVC7_000097"
     CDS             21382..22125
                     /locus_tag="ACHVC7_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2114257.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="ACHVC7_000098"
     CDS             complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="ACHVC7_000098"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR2114258.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            23660..23971
                     /gene="yqfB"
                     /locus_tag="ACHVC7_000099"
     CDS             23660..23971
                     /gene="yqfB"
                     /locus_tag="ACHVC7_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR2114259.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24135..24794
                     /locus_tag="ACHVC7_000100"
     CDS             24135..24794
                     /locus_tag="ACHVC7_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR2114260.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="ACHVC7_000101"
     CDS             complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="ACHVC7_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR2114261.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26213..26479
                     /gene="sdhE"
                     /locus_tag="ACHVC7_000102"
     CDS             26213..26479
                     /gene="sdhE"
                     /locus_tag="ACHVC7_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR2114262.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26460..26867
                     /locus_tag="ACHVC7_000103"
     CDS             26460..26867
                     /locus_tag="ACHVC7_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR2114263.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="ACHVC7_000104"
     CDS             complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="ACHVC7_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR2114264.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27540..28436
                     /gene="xerD"
                     /locus_tag="ACHVC7_000105"
     CDS             27540..28436
                     /gene="xerD"
                     /locus_tag="ACHVC7_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR2114265.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28461..29171
                     /gene="dsbC"
                     /locus_tag="ACHVC7_000106"
     CDS             28461..29171
                     /gene="dsbC"
                     /locus_tag="ACHVC7_000106"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR2114266.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            29177..30910
                     /gene="recJ"
                     /locus_tag="ACHVC7_000107"
     CDS             29177..30910
                     /gene="recJ"
                     /locus_tag="ACHVC7_000107"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR2114267.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31001..32099
                     /gene="prfB"
                     /locus_tag="ACHVC7_000108"
     CDS             join(31001..31075,31077..32099)
                     /gene="prfB"
                     /locus_tag="ACHVC7_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR2114268.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32109..33626
                     /gene="lysS"
                     /locus_tag="ACHVC7_000109"
     CDS             32109..33626
                     /gene="lysS"
                     /locus_tag="ACHVC7_000109"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR2114269.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33669..34217)
                     /gene="idi"
                     /locus_tag="ACHVC7_000110"
     CDS             complement(33669..34217)
                     /gene="idi"
                     /locus_tag="ACHVC7_000110"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR2114270.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34272..34343
                     /locus_tag="ACHVC7_000111"
     CDS             34272..34343
                     /locus_tag="ACHVC7_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114271.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34340..34465)
                     /gene="yqfG"
                     /locus_tag="ACHVC7_000112"
     CDS             complement(34340..34465)
                     /gene="yqfG"
                     /locus_tag="ACHVC7_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR2114272.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34467..35545)
                     /locus_tag="ACHVC7_000113"
                     /pseudo
     CDS             complement(34467..35545)
                     /locus_tag="ACHVC7_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            35981..37900
                     /gene="ygfT"
                     /locus_tag="ACHVC7_000114"
     CDS             35981..37900
                     /gene="ygfT"
                     /locus_tag="ACHVC7_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR2114273.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            37900..38388
                     /locus_tag="ACHVC7_000115"
     CDS             37900..38388
                     /locus_tag="ACHVC7_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2114274.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="ACHVC7_000116"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="ACHVC7_000116"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(38843..39157)
                     /locus_tag="ACHVC7_000117"
     CDS             complement(38843..39157)
                     /locus_tag="ACHVC7_000117"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR2114275.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(39090..42029)
                     /locus_tag="ACHVC7_000118"
     CDS             complement(39090..42029)
                     /locus_tag="ACHVC7_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR2114276.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(42026..42805)
                     /gene="ygfM"
                     /locus_tag="ACHVC7_000119"
     CDS             complement(42026..42805)
                     /gene="ygfM"
                     /locus_tag="ACHVC7_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR2114277.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="ACHVC7_000120"
     CDS             complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="ACHVC7_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR2114278.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="ACHVC7_000121"
     CDS             complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="ACHVC7_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR2114279.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="ACHVC7_000122"
     CDS             complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="ACHVC7_000122"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR2114280.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            48288..49058
                     /gene="yqeC"
                     /locus_tag="ACHVC7_000123"
     CDS             48288..49058
                     /gene="yqeC"
                     /locus_tag="ACHVC7_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR2114281.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            49106..50731
                     /gene="yqeB"
                     /locus_tag="ACHVC7_000124"
     CDS             49106..50731
                     /gene="yqeB"
                     /locus_tag="ACHVC7_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR2114282.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="ACHVC7_000125"
     CDS             complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="ACHVC7_000125"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR2114283.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="ACHVC7_000126"
     CDS             complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="ACHVC7_000126"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR2114284.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(53190..54401)
                     /locus_tag="ACHVC7_000127"
     CDS             complement(53190..54401)
                     /locus_tag="ACHVC7_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR2114285.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="ACHVC7_000128"
     CDS             complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="ACHVC7_000128"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR2114286.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="ACHVC7_000129"
     CDS             complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="ACHVC7_000129"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR2114287.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            57379..59157
                     /locus_tag="ACHVC7_000130"
     CDS             57379..59157
                     /locus_tag="ACHVC7_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR2114288.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="ACHVC7_000131"
     CDS             complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="ACHVC7_000131"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR2114289.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="ACHVC7_000132"
     CDS             complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="ACHVC7_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR2114290.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="ACHVC7_000133"
     CDS             complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="ACHVC7_000133"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR2114291.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            63274..64029
                     /gene="actS"
                     /locus_tag="ACHVC7_000134"
     CDS             63274..64029
                     /gene="actS"
                     /locus_tag="ACHVC7_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR2114292.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            64108..64177
                     /locus_tag="ACHVC7_000135"
     tRNA            64108..64177
                     /locus_tag="ACHVC7_000135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64140..64142,aa:Gly,seq:ccc)
     gene            64382..>65646
                     /locus_tag="ACHVC7_000136"
     CDS             64382..>65646
                     /locus_tag="ACHVC7_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR2114293.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        15770 a        16327 c        17397 g        16152 t
ORIGIN      
        1 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
       61 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa acggcgttat
      121 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
      181 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgggctttct
      241 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctggct
      301 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
      361 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
      421 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
      481 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
      541 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtga
      601 cgggacaaac cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
      661 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
      721 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
      781 ggaaagaccg cgtagatcgg cgatcccttg ttcggtgatg atcactttga cgctgtgttc
      841 gctgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
      901 cgacggagcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccgccgat
      961 gccgttcatc agatcgaccc cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa
     1021 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
     1081 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
     1141 gcgcagggaa tcggccgaga ttgtcaggct ggaggcgctg gccccgctga ttttgccggt
     1201 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcatga acggaggaat
     1261 ttccgggttt tcccccagac gcgccattac cgcattattg atattgccca cgccactttg
     1321 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaata agaacgtgac
     1381 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
     1441 gggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
     1501 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gtgcgccagg
     1561 aatcacaata tccgccagtt ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat
     1621 cactttcttc gcccgcagca gccatgtcgg cgcattaccg atcccgctgg ttaaccagac
     1681 tcgaccatcc ggtgccagtg ccgatgcttc gatgacggca acatcaatgt cgccgaagaa
     1741 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
     1801 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
     1861 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
     1921 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcggtcgg
     1981 taaggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
     2041 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca tttttactcc
     2101 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
     2161 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
     2221 aattcatcgg agttcatggt gtgcgcttcg cccagtacac gcagctcttc tttgataaca
     2281 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
     2341 agttcttcca cttccacaac atggttgagg atgccgacag ccaacgcgcg ctgggcggtg
     2401 attggcgaag cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg
     2461 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggcatcat tgagaaggtt
     2521 gaggtactgg cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa
     2581 acactacctt ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt
     2641 tgacgcaatg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
     2701 tcgtgacctg cggagaagac tttggatcca cttggcgccc gcaaaatgat acagcgaatt
     2761 tccggccggt tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag
     2821 gcattaagtt ttcgtccata gttaaactca atgaccgcca ctttgttgat agtggcaacg
     2881 ttcacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
     2941 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt
     3001 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc
     3061 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact
     3121 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
     3181 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
     3241 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
     3301 gtctttgttg ataacgagca gatcagccac ttccatcagc ccttttttaa tgccctgcag
     3361 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
     3421 cgtttccgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc
     3481 gcataacagc attaattccc gtgctcgctg actggcaccg cccagatgac cggaggaggg
     3541 taccgggcga ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc
     3601 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa
     3661 tccctcacga atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg
     3721 tgtgccggta acgcccagtc gcagggcgtt accacagaac ggcataatgg cgtcaagcag
     3781 ctgtgtactt agcgcctgat gacgtgggtg acggctttcc accagcgtca tggcctgggc
     3841 gagcgtggca cgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
     3901 aatcatgatg ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac
     3961 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa
     4021 tgacgccacc cgcgaccacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca
     4081 gttccgggat cagcgtttta tgatcggcag ccagtgagga tgcgcccact acgtgaacgt
     4141 cgttttctac ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
     4201 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat
     4261 cctggcccat cttagcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
     4321 ccgtttgcgc aacaatggca tcgaactcgg aggccgattt ctcagactga tgatagcttt
     4381 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcgacttcca
     4441 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca
     4501 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
     4561 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
     4621 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
     4681 caacgatgac acgcttgcct tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
     4741 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
     4801 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
     4861 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctggatg atgatctggg
     4921 tgttgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt
     4981 tggtatgcag tgactgagta ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga
     5041 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
     5101 gcagcgccag tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat
     5161 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccgata ccgaagaaga
     5221 acgacaggcg aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt
     5281 actcaatccc atcggcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttccc
     5341 ccatgtggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
     5401 cgatgatgtc ggcgataatg cgcattgacg gttttggtgg gtaaatatag gtgttgcggc
     5461 agaggtactc tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac
     5521 cttgctcttc tgcggcgacg atataaaacg ccagtactgg tagcactgcg ccattcatgg
     5581 tcatcgaaac cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca
     5641 cggtgtcgat agcgacgccc gctttgccga cgtcacccgc cacgcgtggg ttatcggagt
     5701 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
     5761 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
     5821 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
     5881 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agctgcttga
     5941 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
     6001 attccttgtt ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac
     6061 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
     6121 aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt tgtgatgtga
     6181 ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat gtgctatcag gcattatttg
     6241 atggattaat cctgacgaag gactttgtga ccataatcga gcaacgcatc agtgacttta
     6301 cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc
     6361 cctggcgtta agtcaatcag ttcaccgctg gctagttctt tctcgatttg caggtgcggg
     6421 atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga agaattaacg
     6481 atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc
     6541 atatcgtcaa gatggtcaaa ggcgaccaca ggtgctttca gtaatgccga acgcgttacg
     6601 ccgttaggga aatatttttc ggcaaagggt tttgagctga cgaacagata gtcgagcgca
     6661 ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac cgcgccgacc
     6721 acttcgccgc ggcgcagacg ttcctgagta cgggtttcat cttctacctg caagttgagg
     6781 cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt cgccagactg
     6841 tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc atcgcccagc
     6901 cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg cccttgttcc
     6961 gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt ttccagttgc
     7021 ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc gcgctcaaat
     7081 cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt
     7141 gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt tgtgtcaggt
     7201 tgcatatacc gaagccgtcg gcgggtaaca tttgtatccg tggttaaacg cgcagcttaa
     7261 aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgtgccctg gttgtgttga
     7321 atggggtggc gttattgcct caatttgcct gtaaacaggg gcttgcgaac ggggggaact
     7381 ggtgcgcctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
     7441 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cgcggcttgc
     7501 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
     7561 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
     7621 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
     7681 cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
     7741 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
     7801 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
     7861 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgctg
     7921 acgcttccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
     7981 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
     8041 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
     8101 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
     8161 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
     8221 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
     8281 acatgtgtca cgtttttact tggctattgt cgattgctct aaataaatcc tctaaaccag
     8341 catattcaac caagaaatac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc
     8401 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
     8461 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
     8521 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
     8581 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
     8641 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
     8701 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
     8761 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
     8821 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
     8881 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
     8941 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
     9001 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
     9061 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
     9121 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct
     9181 cgctgctgat taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
     9241 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
     9301 atagcgatcc atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca
     9361 ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg ggtaaattga
     9421 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
     9481 cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
     9541 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt
     9601 ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag
     9661 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
     9721 ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
     9781 accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaactca
     9841 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta
     9901 acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca
     9961 tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcagggg
    10021 aggagtcatc ctctcttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat
    10081 cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca
    10141 cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc atgggttttt
    10201 cgagcacgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    10261 gcttacgata gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    10321 tgaaggagaa gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt
    10381 ttcccagctt attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac
    10441 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    10501 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    10561 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    10621 gccagttttg taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact
    10681 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac
    10741 gcacatccag ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat
    10801 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    10861 gcttaccggc gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag
    10921 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    10981 cggcttgttg acccatttcc tgctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    11041 tcattttgcg gatttcttgt cgggataatg tcagtgggag ttctggtagt tgcgtcatgg
    11101 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    11161 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacggac cgagcatgct
    11221 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    11281 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    11341 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    11401 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    11461 cttcgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    11521 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    11581 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    11641 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    11701 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    11761 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    11821 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    11881 accagtatct gaaccaacaa gggacggggc tgacccctgc cgagatgcat ggtttaatca
    11941 gtgggatgat atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga
    12001 cgaacgaagg catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg
    12061 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    12121 atgatgtcag cgttttcgat cgggctgatg cattggcagg ttgggtcaat cacttcctgc
    12181 ttggtcttgg cgttacgcaa ccgaagctgg ataaagtgac cggcgaaacc ggtgaagcta
    12241 tcgacgatct gcgtaacatt gcgcaactgg gttacgacga agacgaagat caggaagagc
    12301 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    12361 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    12421 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    12481 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    12541 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    12601 ttcaccggct ttaacgaatc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat
    12661 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    12721 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    12781 gaaatcaatc agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag
    12841 ggcgaatatg catatgctga tgaaatcatg aacagtgcgc tggaaaaact gcgtaaaggt
    12901 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    12961 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    13021 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    13081 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccgtc ctataacacc
    13141 attgtcggca gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg
    13201 cgcgacggtg acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat
    13261 attacccgca ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac
    13321 attgtgctgg agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg
    13381 gaagtcactg gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg
    13441 aaaggtgatg ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    13501 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    13561 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    13621 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    13681 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    13741 gcgttgatgg cagctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    13801 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    13861 ttgaagcgac cgcgccagag tcacatgctc atccgggctt tgatggacga gctattgcgc
    13921 tggctgcggg gacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggcggatt
    13981 acgcaaccgc catcactacc gtgcatgtca gcgatcgtgg tcacgctgga tttgtcaccc
    14041 tcgccgcaga agattaccaa ctggcggcgc tgggacaggt tgtcgaattg cacaatgtcg
    14101 ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt aacgctgcat tgccctgatc
    14161 gcgtggctaa cgttgcccgt actcagagtc acgttgaagt gacgctggag agtggcgaga
    14221 cgctgacggg ccgcgtgctg gtagcagctg atggcaccca ttcagcgtta gccaccgcgt
    14281 gcggcgttga ctggcagcag gagccttacg aacaactggc cgtgattgcc aacgttgcga
    14341 cttccgttgc gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga
    14401 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    14461 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtcg gcctttggct
    14521 ggcgacttgg aaaaattacc cacgccggta aacgcagtgc ttatccgctg gcgttaaccc
    14581 gcgccgccag agctattacc catcgtaccg tgctggtggg caatgcggcg caaactctgc
    14641 acccgattgc cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa
    14701 ccctgactca ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct
    14761 atcagcagcg tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac
    14821 atctttttgc caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga
    14881 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    14941 gttgaaggcg gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggtggc
    15001 ggcatggtgg ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta
    15061 ctggagcagc gcgtaccgga acctctggcg gcggatgcac caccacaact gcgcgtttcg
    15121 gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc
    15181 tctcgtaggg ccagttgtta tcacggtatg gaagtgtggg acaaagacag ttttggtcac
    15241 atttcgtttg acgatcaaag catgggctat agccatcttg ggcatatcgt tgaaaactca
    15301 gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatatcac tctgttggcc
    15361 cccgcagaat tacagcaggt cgcctgggga gaaaatgaaa ctttcctgac gctgaaagat
    15421 ggcagcatgt taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc
    15481 aacaaagcag atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc
    15541 attcgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt
    15601 ctggcctttt taccgcttag cgatccgcat ctttgctcaa ttgtctggtc actgtcgcca
    15661 gaggaagcgc agcggatgca gcaggcaagt gaagatgaat ttaatcgcgc gttaaatatc
    15721 gcttttgata atcgcctggg cttatgcaag gttgagagcg cgcgtcaggt gttcccactg
    15781 acggggcgtt atgcgcgcca gtttgccgcg caccgtctgg tgctggtggg cgacgccgcg
    15841 cataccattc acccgctggc ggggcaggga gtaaatcttg gctttatgga tgctgcagag
    15901 ctgattgctg aactgaaacg gttgcatcgt caggggaaag acatcgggca gtacatttac
    15961 ctgcgccggt atgagcgtag ccgcaagcac agtgcggcgc tgatgctggc tggtatgcag
    16021 ggattccgcg atctgttttc cggtaccaat ccggcgaaaa aactgctgcg tgatattggt
    16081 ttgaaactgg ccgacacgct tcctggcgtt aaaccacaac ttatccgcca ggcaatggga
    16141 ttaaacgatt tgcctgaatg gctgcgttaa aaatttctct tctgctgttt atttgatacc
    16201 catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta
    16261 atttcacctc cgttttcgca ttatattttc taatgtcatt attttttgat ttagtgtttt
    16321 ttgacatttt tttagctctt aatattgcct tattcaaatt agctttctaa tggcatcatc
    16381 tttatataga aactggtgtg ttttttggtt ttttattctg tcgcgatttt ggcgtttttt
    16441 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
    16501 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc
    16561 gtggtgataa tttcaccatg aaaagttgtc agccccgctt attcaatgag gacaagatgg
    16621 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    16681 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    16741 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatctccgc ggcagccgca
    16801 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    16861 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct
    16921 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    16981 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    17041 tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
    17101 acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
    17161 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    17221 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg tgttaagcca tgtggcttgg
    17281 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    17341 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccggcagatc
    17401 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    17461 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    17521 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    17581 gttacagcat tgcgctggcg cgcgtgctgg aaggtattgg cgaaacggcg attgtgcaaa
    17641 ttcgcaaccg tgaaatgtcg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    17701 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    17761 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    17821 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    17881 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga
    17941 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    18001 ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca aagccagcga
    18061 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga
    18121 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    18181 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    18241 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    18301 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    18361 cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg caggttggcg
    18421 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    18481 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    18541 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
    18601 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    18661 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    18721 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
    18781 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    18841 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    18901 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    18961 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    19021 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    19081 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    19141 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    19201 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    19261 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    19321 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    19381 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    19441 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    19501 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagcgtgc
    19561 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    19621 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    19681 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    19741 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    19801 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgcccgccgg
    19861 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    19921 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    19981 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    20041 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    20101 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    20161 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    20221 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    20281 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    20341 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    20401 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    20461 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    20521 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    20581 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    20641 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    20701 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    20761 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    20821 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    20881 cagaaattga ccaggtgaaa gccgctgtct ggccgctgga agataacccg ctggtgaacg
    20941 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    21001 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    21061 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    21121 ctgattcgac tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    21181 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    21241 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
    21301 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
    21361 taatttttct cggaaaaaca aatggctata gcacttgtga ccgatggcag tcgcggcatc
    21421 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
    21481 caaaatctcc atgcggcgca ggaagtggtg aacttaatca cgcaagccgg tggcaaggca
    21541 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
    21601 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
    21661 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacatgacg
    21721 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
    21781 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
    21841 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
    21901 gtcgccgcgc agggggtccg cgttaactgc gtgcggccag ggtttatttt taccgaaatg
    21961 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
    22021 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
    22081 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
    22141 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
    22201 ctggtaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    22261 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggtg
    22321 gtgaacgata cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    22381 taagtcaccg cttttttcat ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg
    22441 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    22501 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
    22561 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcgaaa
    22621 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
    22681 aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg
    22741 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
    22801 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
    22861 gggttacagc agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
    22921 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    22981 acctgataca tcgtctcttc cgggttctca tgctcggtat aaaccacgcc ggagcagcag
    23041 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    23101 tatttgactt tatgcttata gcgttcaaac accacttccg cgaaacggac aaagaaatca
    23161 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    23221 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    23281 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    23341 caggctatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    23401 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
    23461 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    23521 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    23581 cataagaaat ctttctgtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    23641 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    23701 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    23761 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
    23821 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    23881 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
    23941 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    24001 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    24061 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    24121 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    24181 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    24241 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    24301 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    24361 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    24421 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    24481 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    24541 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    24601 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttattggcg gtaggcggcg
    24661 tggtttattc gctcggagtg attttctatg tctgcaaacg cattccctac aatcatgcca
    24721 tctggcacgg cttcgtgctc ggcggcagtg tgtgccactt tctggcgatc tatttgtata
    24781 ttgggcaggc gtaatgggta tgcctttgta ggcctgaaaa gacgcgtcag cgtcgcatca
    24841 ggcatcggag cacaactgcc ggattcggcg tgaacgcctt atccgatcta cgggcctcgt
    24901 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    24961 gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg cagcggctca
    25021 atatgcaatg tattcgcatc gtcgcgtacg cggaagatgc tatccggttc catatcgtta
    25081 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    25141 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    25201 cggctggcgc tacctgccag caaccagagc gcacgtttat tggcaccacg gaattttgct
    25261 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    25321 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    25381 cccgcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    25441 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    25501 gggtattcaa accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctttcgaa
    25561 ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    25621 agcacatgct cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt
    25681 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg
    25741 cggaacagac gtaaattgct ccacattttg cctttggcgt cgcaatgggc ggcgagcagg
    25801 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    25861 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    25921 aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc
    25981 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    26041 ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agccattgaa
    26101 ttgttgtgaa ttaatttcgt gatcgcgatg gtgtttactg atattcccgt aaaagaaggc
    26161 gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga agatggacat
    26221 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    26281 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    26341 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    26401 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
    26461 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    26521 ttcatgggct ggttgccgct gttattttac tcatgccctg gccactcagt tacaccccgt
    26581 tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta
    26641 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    26701 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    26761 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    26821 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    26881 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    26941 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    27001 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    27061 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    27121 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    27181 gcaacaattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    27241 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    27301 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    27361 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    27421 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    27481 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    27541 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    27601 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    27661 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    27721 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    27781 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    27841 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    27901 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    27961 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    28021 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt
    28081 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    28141 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    28201 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    28261 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    28321 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    28381 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    28441 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    28501 tttcaggttt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    28561 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    28621 gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    28681 ttagcggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    28741 ttgaaaaaga gatgatcgtt tataaagcgc tgcaggaaaa acacgtcatc accgtgttta
    28801 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    28861 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    28921 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    28981 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    29041 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    29101 cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa aaaatgacca
    29161 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgcag tcgatgaaac
    29221 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggcgt
    29281 gcgcagtgcg caagaactgg aacgcagtgt taaagggatg ctgccctggc agcaactgag
    29341 cggcgtcgaa aaggccgttg agatccttta caacgccttt cgcgaaggaa cgcggattat
    29401 tgtggtcggt gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat
    29461 gcgctcgctt ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta
    29521 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggtgcgcagt taattgtcac
    29581 ggtggataac ggtatttcct cccatgcggg ggttgaacac gctcgctcgt tgggcattcc
    29641 ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcggcgg aagcgatcat
    29701 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    29761 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    29821 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtggc
    29881 ggacgtcgta ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
    29941 ccgtgccgga aagtgccgtc cggggattaa agcgctgctt gaagtggcaa accgtgatgc
    30001 acaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
    30061 acgactggac gatatgtccg tcggtgtggc gctcttgctg tgcgacaaca tcggcgaagc
    30121 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    30181 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
    30241 cggcgggctg gcaatgtatc accccgaatg gcatcagggg gttgtcggta ttctggcttc
    30301 ccgtattaaa gagcgttttc accgtccggt tatcgccttt gcgcccgcgg gtgatggtac
    30361 gctgaaaggt tctggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    30421 agacacactc taccctggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt
    30481 gtcgctggaa gcggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    30541 gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    30601 cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    30661 cccggagccg ctgtttgacg gtcatttccg gctgctgcaa cagcggctgg tgggcgaacg
    30721 tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg ctgctggatg gtattgcttt
    30781 taatgtcgat accgccctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa
    30841 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    30901 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    30961 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    31021 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    31081 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    31141 ggaatgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    31201 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    31261 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    31321 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    31381 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    31441 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    31501 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    31561 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt
    31621 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    31681 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    31741 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    31801 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    31861 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    31921 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    31981 tccttgatga ctcccgcatt aaagatctgc gtaccggggt agaaactcgc aacacgcagg
    32041 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    32101 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    32161 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    32221 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    32281 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    32341 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    32401 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    32461 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    32521 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    32581 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    32641 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    32701 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    32761 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    32821 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    32881 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    32941 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    33001 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    33061 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    33121 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    33181 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    33241 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    33301 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    33361 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    33421 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    33481 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    33541 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    33601 tcccggcgat gcgtccggta aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    33661 ggggtttttt aattgtgctg cgcgaaagca gacaacaatt ttcttgcttc actattggct
    33721 gcctgcatca ccatccacgg actgaacgcc cacggtgtgg catcaatacc gtgtaataca
    33781 tctgctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    33841 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg
    33901 gcgcgatagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    33961 taacggcaac ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa
    34021 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    34081 aattgtcctt tggcattaaa cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc
    34141 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    34201 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    34261 tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat
    34321 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    34381 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga
    34441 atataacgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    34501 atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc
    34561 accaggtcag catgatgctg gtagccatta aagaagacat tcagcacaac ggcgctaagg
    34621 gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag
    34681 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    34741 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    34801 atgccgaaca tcaccagccc agcaccgccc agcacaaatt gcggaatgga ggcgaccagc
    34861 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    34921 cagcggctat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacagcaa
    34981 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    35041 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    35101 gtggcgggaa taaaggcatc aagcgaccaa taaatggcgc taataatgtg gtgataaaac
    35161 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    35221 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    35281 taaagcggcc gataccgata cgttgcaata atgtgacgat cccgcagcaa aagagatccg
    35341 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    35401 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    35461 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    35521 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    35581 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    35641 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    35701 aatgacaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    35761 atcattctgg catgtcgcac tctagcattt aatcgttttt atctggatag cgctcttttg
    35821 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    35881 cgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    35941 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    36001 tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa
    36061 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    36121 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gtgttacggc
    36181 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    36241 tattggctgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    36301 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    36361 agtctgccca acacaggcgt tacgactgat ggacgataaa gggttacagt ggataaaggt
    36421 ggctcgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    36481 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    36541 gaaaacccac tttggcgaaa tctattgcgg gctggatcca ctgcaagcga cttatgagag
    36601 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa
    36661 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    36721 ttgccaccag accagttcct taccagaaat ctgcggcagg gtatgcccac aggaccgtct
    36781 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    36841 acgctacatc accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    36901 tccccgtatc gaaaaagtgg cggtgattgg cgctgggcca gcagggttag ggtgtgctga
    36961 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    37021 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    37081 agagatattc accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gccgcgatat
    37141 tacctttagc gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg
    37201 gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt
    37261 cctgactgcc catacccgcc agctcatggg attgccggag tctgaagagt atccgctgac
    37321 ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt
    37381 gcggacttcc attcgcctca atgctgccag cgtgacctgc gcgtatcgtc gtgatgaagt
    37441 cagtatgccg ggctcgcgca aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca
    37501 gttcaatgtt caaccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg
    37561 cctgattcgt accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt
    37621 agcgggttca gagtttgaat tgcccgccga tgttctgatt atggcctttg gtttccaggc
    37681 gcatgccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    37741 aaccggtgac gtcgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg
    37801 tgatgcagtt catggcgcgg atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    37861 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    37921 aatccggctg actgtattgg ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct
    37981 tcagaacagg agttgaatgc cgatgtcttt ttaccccggc tgaaggtaca gcgactggac
    38041 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc
    38101 cccgtggggg cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt
    38161 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcgttgccg
    38221 ggggataccc gacaacaaat tgtgaaatgc gatctctgtg aacagcggga agaggggcca
    38281 gcctgtgttg aatcttgccc gacacaggcg ctgcagttgc ttactgaaag agaactcagg
    38341 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg
    38401 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt
    38461 gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa tgccgtcaag
    38521 gaaatcgcac aaccaatcgc cattggcaaa ttggcctaca accccatcag caacgagccg
    38581 aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc
    38641 agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc
    38701 agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg
    38761 ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca
    38821 cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg
    38881 tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag
    38941 aaacagagtt gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga
    39001 aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg
    39061 ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt
    39121 gcaacacttt gtaggcttcg ttcagcgttt gcagcatgtt gaaagttgtt ccggcaccga
    39181 tatccgttcc catgcccact ttgactttct tctgcccaga tgccgcatgc atcgtgaatt
    39241 gcggtagcaa tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg
    39301 aacgggccga ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca
    39361 ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca
    39421 taaatgatct cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt
    39481 cccaatgcta attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc
    39541 aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg
    39601 aaggcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccgatgccg
    39661 gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg
    39721 cccggcgttg ccagttgaac atctgccact gcctcaccta acatttgcgc gccgtggaac
    39781 agaattttct cccgtagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta
    39841 ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca
    39901 tgcacgtcct gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc
    39961 agaccagtac caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt
    40021 ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg
    40081 acgccgcgac cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc
    40141 tcgcgaccct gacgcaggat ctcttccagt gcacagctgg cggcggaagg aacggaggtc
    40201 ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg
    40261 ttacgttcga taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc
    40321 atggtgatag cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa
    40381 atgttgctgt agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc
    40441 gacagcgccg gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg
    40501 gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg
    40561 gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg
    40621 aatagtaccg gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg
    40681 tcctgtttgg aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt
    40741 ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg
    40801 tggataacca gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac
    40861 tgctgtgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct
    40921 ttgtccatat cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa
    40981 ccgatcggga agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct
    41041 tccagagtat ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca
    41101 tcttccgcca ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg
    41161 atgagcttca gtgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga
    41221 tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt
    41281 gcgctctgac cccccggggt atagtagata tcagggcaat tcaggtgagt aataacgtga
    41341 acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg
    41401 tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcagtc ttcaacatag
    41461 cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgacg
    41521 tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt
    41581 gcaaccgcca gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa
    41641 ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg
    41701 gtgataatca gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc
    41761 attgcctgtt gaaccagact taactcgttc catttaccca gagattctgc ggtacgaata
    41821 tctgccttct ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag
    41881 attattgcgt cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt
    41941 cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc
    42001 gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg
    42061 catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca
    42121 aatcttcctg cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat
    42181 tttgtttttc gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc
    42241 gcataccgtc gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag
    42301 aacggctaat tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta
    42361 gcaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa
    42421 aaaccagttc agcatccagt gctagcagga cgggaagcag cactgactct tcctgccggg
    42481 cggcgatttc accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga
    42541 gggcttcacg cagtgctgca ggaataaagc gcgcatcacg cagtggctgc aagcgagaca
    42601 ttgcaccaat ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta
    42661 aggaaatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg
    42721 cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt
    42781 cgggcctgaa aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa
    42841 gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt
    42901 tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat
    42961 cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc
    43021 ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg tcaaatcagc
    43081 tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt
    43141 accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg
    43201 tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa
    43261 tgccaggttg cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga
    43321 acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga
    43381 caagtacagc ccatgagcga ccagcgtttt gctgtcgatg aggtcgaatt gcgccagtcg
    43441 tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc
    43501 agcgtgaata tgcaaaccac ggcctgtggc tttcacggct tcacgcagca tctccagacc
    43561 ggcatccggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc
    43621 gcttgtcgct ttcttcgcct catcaatcaa acgggcgaaa cggatgtttt cttctacacc
    43681 ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg
    43741 caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg
    43801 cgaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat
    43861 cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt
    43921 tttcagcgtt gagatgaaat ccgggcaagg ggcgatgttt gccataattc cgcgggaaag
    43981 ctccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat
    44041 ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac
    44101 gtcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc
    44161 ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga
    44221 tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgtta
    44281 aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc
    44341 cggcaatctt ccacaaggaa gcctggggtg ctgctgttat caaagtcttg cgccaggctg
    44401 aagacggtga ttttgtcttc gtatggttta ccgttccacg gacagaactg agcgcagttg
    44461 ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagccaggg
    44521 actgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgtt gcaaacgtag
    44581 ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc
    44641 accagcgtga tagagatatc gcctttacgt tgatagattt ccgctggatt gacgttgttc
    44701 cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc
    44761 cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc
    44821 ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca gccgtttttg
    44881 tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg
    44941 atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacgacct
    45001 ttctcatccg gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa
    45061 cgttccggat tattcaggaa acggaactct acgccgtcgt gcaacgcttc ttcatactct
    45121 tcgcgccatg cgggcatctc ttgtagtgaa cgacggtaaa cgatcgttgc tttttctacg
    45181 cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg
    45241 accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt
    45301 gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt atcagtgccg
    45361 gtggcaatca gaacatagtg gaagccctga ttttttaact gttcaacggt taaatcgggt
    45421 gagcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt
    45481 aactctgcag gaatacggaa ctgaggaatg atatttttca ccacgccgcc agcattggct
    45541 tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga
    45601 cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggcgggtttg
    45661 tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg
    45721 atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa
    45781 atatgaccgg taatggcagg tagggcgttg cgttggtaga tgagttccag cgcgtcggca
    45841 tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca
    45901 gtaacacagg gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc
    45961 tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc tgccagtctg
    46021 ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc
    46081 atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catagtaata
    46141 gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa
    46201 gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc
    46261 gggaacagcg cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg
    46321 atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt
    46381 accatcaggc gctccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac
    46441 tcttctttta agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg
    46501 taccccagta aggtcgggtt aagtttgaca aaggtgttga gccctttttc ttccagcatg
    46561 tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg
    46621 ccctgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagttttc gcgtttttcc
    46681 tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga
    46741 tattgagcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatc
    46801 ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta
    46861 ccggaatcag aaggctgtaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg
    46921 tattcatccc aggctttaag caaggtaaat tcggtagacc attcggtgtt aaagcattcg
    46981 tcttcggcat caatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt
    47041 agttcgatga agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg
    47101 tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt
    47161 ttacctttta caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt
    47221 tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc
    47281 cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg
    47341 gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata
    47401 aaaaaatgtg aggtggctcg aaacttaaag gctttttgtg aatgaactca caggtgccaa
    47461 atgagaatta aaatcactcc tgaaaattat tattgtcagc ccatttatca ctttttctct
    47521 tttctcaaaa tcagtgattc cttatcatta tgataaagag tgctggagag attaaatatt
    47581 tgaacgcaat cgtttaacta cattttctaa attttagtat accttttttg cgctgtaata
    47641 aagtcgtccg gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa
    47701 tgatctccca taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc
    47761 atcaggcatg gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt
    47821 attgcgccat catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc
    47881 atgtcaccgt gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgacg
    47941 gatgttagta aaccctgcgc ataatctggg ttgtgaataa tagtgatatt gctctggttc
    48001 gcataacgtt cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa
    48061 aactgcaacg catttttgat acttgtatca agaattgttc cctgttccca ggggaacatc
    48121 attttccatt gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct
    48181 gacatagtgg ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg
    48241 atggattaag ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag
    48301 ttgacccatc ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg
    48361 ttgttggtgc tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta
    48421 gcggcaggcg tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc
    48481 ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt
    48541 cattttgttt tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag
    48601 cgattgatgc actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct
    48661 cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt
    48721 gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg
    48781 tccatcgctg gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac
    48841 tgagcgatct cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag
    48901 gttgtcggcg agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa
    48961 gcgagctcct tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac
    49021 aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac
    49081 tgaaaggata tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga
    49141 gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca
    49201 ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat
    49261 ggttgagtgg aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt
    49321 attccatgga cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc
    49381 aatgtcagta tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg
    49441 gcatgtcaac cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc
    49501 cgatatttat cgcgaaagcc tcaatcctga actattccca ccatcaacca cgcttctcca
    49561 tgctgagtcg tttggtgcgg cagtggaaga actggatatt cgccctgata attttgtcct
    49621 gattgccacg aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc
    49681 atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga
    49741 gaaaggcgtg gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg
    49801 tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca gaaatattac gggtgaaaaa
    49861 taacgcgccg ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt
    49921 gattcgcggt gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt
    49981 taaagtgatc atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc
    50041 ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag
    50101 ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg tagatcccgc
    50161 ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca
    50221 gaatttggga acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac
    50281 tgcagggaag gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt
    50341 gatttactct ggttgtgcgc aggagaatac cgatgttcct ggcaatatta tggggcatac
    50401 cacccgacgg gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg
    50461 cgatctggtg aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc
    50521 gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg gcagtggttg gtggttttaa
    50581 aattggtgat atcgatcctc gtggtgaaac cgctgatttc accagcgttt ctgataaagc
    50641 ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa
    50701 agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc
    50761 cggccttaaa attactgaca aatcaccgtt ccggttttgc cttcaatccc ttctttcgct
    50821 ttgctcaata cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca
    50881 gcttccactt taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca
    50941 gcatcactta atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc
    51001 actttttcta ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg
    51061 gcgctggccc agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga
    51121 ataacaggga taccaccacc gccaacggcg atcaccacct ggcctgcatc taccagagct
    51181 ttaaccgttt ctttttcaat gatatcaacc ggttttggcg aggcaacaac acggcgatag
    51241 ccgcgaccag catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc
    51301 tcggtaaaga acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg
    51361 acttcgacct gagtaaccag cgttgctaca ggtttattaa tgccccgaga aagcagttct
    51421 tctcttaacg cgttttgtag atcgtaacca atataaccct ggcttaaggc aacacagaca
    51481 gacatcggca gcattggcga gtgtgcttca gtttttgcgg cagcttcaaa ggcttggtta
    51541 atcatgccca cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt
    51601 aaatcaacaa ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg
    51661 cccagcgcat tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca
    51721 ttcttgattg tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc
    51781 gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt
    51841 caccacggga taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt
    51901 agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa
    51961 tcaccacgtc gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca
    52021 gcctggcggg cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca
    52081 tcacgccact ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc
    52141 agcgactgaa atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat
    52201 cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc
    52261 gtacgttacg cagcggtggg ctaagaatga acttcatgcc atcttctgta tcgtaactgc
    52321 gttcgtccaa caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac
    52381 gggcaagacg cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg
    52441 cgttaccggc aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca
    52501 gagggcgact caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc
    52561 gcttgctggc gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat
    52621 gcaagcggcg taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat
    52681 agagtttaaa actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt
    52741 gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat
    52801 aaccacgata aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat
    52861 ggtcaataat ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat
    52921 cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag
    52981 ggaaaacgta acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt
    53041 tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg
    53101 cgttaacgac agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg
    53161 cgaaccggag gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt
    53221 gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca
    53281 tgcgcttcag gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg
    53341 atagatacgc cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc
    53401 ccttcgtagg cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc
    53461 gggaagtagc attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac
    53521 atagaaacaa ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg
    53581 ccttcccagg tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct
    53641 acagcgcaac ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg
    53701 aggaattcgt cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc
    53761 attttgaaaa tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga
    53821 acatcaatac gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt
    53881 tcggtactga ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac
    53941 agaccgtcgc agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc
    54001 agaccgaggt ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg
    54061 tcggaagtac cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag
    54121 tcccagtttt tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga
    54181 cgcgggccgt ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg
    54241 aagccgactt tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt
    54301 tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt
    54361 gctttttcaa gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt
    54421 tactccccgt tttgcaggga gtagttccaa tagggtgatt aaggtgctac agcgtgtttg
    54481 ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc
    54541 agcactacgg catctttgtc cagcgccagt ttttccatca ggctttgatg ttgcgggtga
    54601 taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc
    54661 gggtcgttgc cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg
    54721 caggagatga attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg
    54781 ccacaggcca ggcccgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct
    54841 ttgacgccgg agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga
    54901 ttttgcgggc tgtagacgtc gaccagataa cccagcacac caccggccat tgctccgaca
    54961 ccggcttgca gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct
    55021 tcatctgcca gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc
    55081 catgccgtgt cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga
    55141 acggtatcgt catagttcat atccgtgacg atgcactcgg caccgaggtt cagaatggcg
    55201 tcaacgcgtt cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc
    55261 tgtgctgccc acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt
    55321 ttttcgccga tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat
    55381 ttttcacaca ataattgagc gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga
    55441 ccgaatcgtt ttgattcgtc tttgacgaga attttcttca caccaaaaag gtttgcgaga
    55501 tcgtccagcg cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc
    55561 gccagtttgg cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta
    55621 tcggcgatat caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa
    55681 tagagggccg accagcagat cggcccgagg ggttatttca cgcgttcttg cgcttctttc
    55741 agcagttgct cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg
    55801 atgatataag gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg
    55861 ccttcagtca cttcaccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc
    55921 atgtacaggg cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct
    55981 ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtggtcg
    56041 ttcgcacgca gcaattcagt acgctcttcc atcactttgt aaggtgccca tgacttcgga
    56101 taaacgatgt ctgcgtcttt gaaggcttct tccatgctgg tgacctgacg gaagctacca
    56161 ccggaggctt tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct
    56221 tccggatggg ccaaggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt
    56281 ggtacagaga gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta
    56341 cctttcaggt tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac
    56401 tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca
    56461 ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg
    56521 cccagataca tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg
    56581 gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt
    56641 gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg
    56701 cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc
    56761 agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc
    56821 cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc
    56881 agctcattaa cagttttcat catacagtcc cggtagtgga atgacaggca aagtggttta
    56941 tttcactgtg acttgccaaa gtgattggtg ttttcatagc tatacctggg actaacaata
    57001 agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa tgtgtggggt
    57061 tgttcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt ttggcttttt
    57121 ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc aggctatctc
    57181 tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc
    57241 ttccataatt gcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc
    57301 tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta
    57361 agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa
    57421 ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagtttga ggttgagatc
    57481 gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc
    57541 cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt
    57601 gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc
    57661 gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt
    57721 ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct
    57781 gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca
    57841 ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta
    57901 gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt
    57961 gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt
    58021 aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc
    58081 attggtcaat tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg
    58141 cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc
    58201 gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc
    58261 agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat
    58321 aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag
    58381 cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactag tgcttctgcc
    58441 aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata
    58501 ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt
    58561 ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat
    58621 cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt
    58681 aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg
    58741 gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct
    58801 cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc
    58861 aatttgatgg aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta
    58921 ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt
    58981 gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa
    59041 atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc
    59101 atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct
    59161 cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag
    59221 aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg
    59281 acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc
    59341 cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg
    59401 ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcctttcc
    59461 ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt
    59521 acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg
    59581 gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat
    59641 ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg
    59701 acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta
    59761 ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc
    59821 agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc
    59881 ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt
    59941 cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta
    60001 aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt
    60061 tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa
    60121 cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca
    60181 gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc
    60241 tgcggtccag caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata
    60301 ggagcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc
    60361 gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg
    60421 tcattgtgat ggtggagctg aatcagtacg tcagtgccat cggcgagcag tttggcctgc
    60481 gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca
    60541 aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg
    60601 ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg
    60661 aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga
    60721 ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg
    60781 cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat
    60841 ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc
    60901 cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac
    60961 tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccgatgggtt
    61021 agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa
    61081 agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac
    61141 gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg
    61201 agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata
    61261 gctctggcgt gaggcaaatg cgccgggagc gaacggcgta acgtcggtat cttgagttga
    61321 aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac
    61381 ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg
    61441 attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtata
    61501 gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc
    61561 acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc
    61621 gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa
    61681 acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac
    61741 ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt
    61801 atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt
    61861 cctccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac
    61921 atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt
    61981 aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac
    62041 cggaatgccg ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca gtacatcctg
    62101 tttattacca aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat
    62161 atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat
    62221 ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg
    62281 ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg
    62341 ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg
    62401 tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc
    62461 aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc
    62521 aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg
    62581 cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat caggcacatc
    62641 ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact
    62701 tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat
    62761 aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat
    62821 gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac
    62881 atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga
    62941 taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa
    63001 caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga
    63061 taaattggga ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata
    63121 cctctacagc gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt
    63181 ctctattacc acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga
    63241 taaggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa
    63301 tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc
    63361 aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg
    63421 ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc
    63481 atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt
    63541 agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg
    63601 tcatcagcgg taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca
    63661 acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat
    63721 atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg
    63781 ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt
    63841 acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg
    63901 caaaaaatcg ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt
    63961 cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca
    64021 aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa
    64081 ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc
    64141 ccaagctcta tacgagggtt cgattccctt cgccgctttt gctgtatctg attttctata
    64201 ttatcttaat tcaatcgctt cagacttttc gcaatcgccg taatatttcc ccattttgtt
    64261 gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aacccaggta aaacacctta
    64321 ttttcaagtt gttaaataag tggttattta cgcttttaac aaacgattga tggaatattt
    64381 tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg
    64441 ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg
    64501 ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtacccta attatcgtct
    64561 tctgattcac tcagccaaat taaaaaatga aataaagagc cactatacca aagacgaaat
    64621 tcaagggttg ttaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg
    64681 gggaacattt attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta
    64741 tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat cagatcaggg
    64801 aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgctcga
    64861 ccagattaag cggatgcagg acgtcattag caaccccaaa cgactggatg gagttcccgg
    64921 gcaaatgaat gctgtgcata tccgatttga cagcaactcc cccgtgatgg cagatgtgca
    64981 ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag gtctttatct
    65041 tgatctcctt atacaagcaa tcgacactgg tacaattaac gctgaagatt ggcaaaaagg
    65101 tgaccgatta aaatcggttg cgctgctgat tgcttatctg gataaagcca atttctatgt
    65161 tatggaagat tccggagcat gggaagaaga tgcccgatta aacacatctt ccgttgcact
    65221 ggtgacctct ggactggaac gtttgtcgaa cttactctcg aagaaagact ctgtttttgt
    65281 ctctgattta ctacgcgaag ctaaagccaa tgaactggat gaacctcttt caacaacgag
    65341 gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg atttaggggg
    65401 agaatcccca ggttatctgg aaaaagataa acattacaga gaagccgatg cggctttact
    65461 taatgttatt tatcctgcaa atcttgcaaa gataaacacc agaagaaaag agcaggttct
    65521 aaaaatagtt aaaaaactag caggtccgta tggtataaaa agatacgaga aggataatta
    65581 tcagtcggca aatttttggt tcaatgatat taaaaccgat accgatcaaa attcacatgc
    65641 gaaaag
//
LOCUS       DAZPLM010000003        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPLM010000003 DAZPLM010000000
VERSION     DAZPLM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.003"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHVC7_000137"
     CDS             <1..468
                     /locus_tag="ACHVC7_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114294.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHVC7_000138"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHVC7_000138"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR2114295.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHVC7_000139"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHVC7_000139"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR2114296.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHVC7_000140"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHVC7_000140"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR2114297.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHVC7_000141"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHVC7_000141"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR2114298.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHVC7_000142"
     CDS             complement(4084..5313)
                     /locus_tag="ACHVC7_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR2114299.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHVC7_000143"
     CDS             complement(5377..5817)
                     /locus_tag="ACHVC7_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR2114300.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHVC7_000144"
     CDS             complement(5922..6692)
                     /locus_tag="ACHVC7_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2114301.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHVC7_000145"
     CDS             complement(6689..7615)
                     /locus_tag="ACHVC7_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2114302.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHVC7_000146"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHVC7_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR2114303.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHVC7_000147"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHVC7_000147"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR2114304.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHVC7_000148"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHVC7_000148"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR2114305.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHVC7_000149"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHVC7_000149"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR2114306.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHVC7_000150"
     CDS             13322..13669
                     /locus_tag="ACHVC7_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR2114307.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHVC7_000151"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHVC7_000151"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR2114308.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHVC7_000152"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHVC7_000152"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR2114309.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHVC7_000153"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHVC7_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR2114310.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHVC7_000154"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHVC7_000154"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR2114311.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHVC7_000155"
     CDS             18978..20735
                     /locus_tag="ACHVC7_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR2114312.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHVC7_000156"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHVC7_000156"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR2114313.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHVC7_000157"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHVC7_000157"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR2114314.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHVC7_000158"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHVC7_000158"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR2114315.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHVC7_000159"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHVC7_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR2114316.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHVC7_000160"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHVC7_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR2114317.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHVC7_000161"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHVC7_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR2114318.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHVC7_000162"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHVC7_000162"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR2114319.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHVC7_000163"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHVC7_000163"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR2114320.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHVC7_000164"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHVC7_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR2114321.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHVC7_000165"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHVC7_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR2114322.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHVC7_000166"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHVC7_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR2114323.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHVC7_000167"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHVC7_000167"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR2114324.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHVC7_000168"
     CDS             complement(36854..36898)
                     /locus_tag="ACHVC7_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114325.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHVC7_000169"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHVC7_000169"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR2114326.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHVC7_000170"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHVC7_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR2114327.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHVC7_000171"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHVC7_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR2114328.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHVC7_000172"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHVC7_000172"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR2114329.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHVC7_000173"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHVC7_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR2114330.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHVC7_000174"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHVC7_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR2114331.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHVC7_000175"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHVC7_000175"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR2114332.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHVC7_000176"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHVC7_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR2114333.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHVC7_000177"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHVC7_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR2114334.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHVC7_000178"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHVC7_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR2114335.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHVC7_000179"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHVC7_000179"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR2114336.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHVC7_000180"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHVC7_000180"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR2114337.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHVC7_000181"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHVC7_000181"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR2114338.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHVC7_000182"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHVC7_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR2114339.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHVC7_000183"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHVC7_000183"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR2114340.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHVC7_000184"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHVC7_000184"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR2114341.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHVC7_000185"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHVC7_000185"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR2114342.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHVC7_000186"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHVC7_000186"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR2114343.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHVC7_000187"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHVC7_000187"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR2114344.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHVC7_000188"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHVC7_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR2114345.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHVC7_000189"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHVC7_000189"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR2114346.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHVC7_000190"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHVC7_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR2114347.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16851 c        15001 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacta gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacatccgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZPLM010000004        48930 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPLM010000004 DAZPLM010000000
VERSION     DAZPLM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.004"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(85..1032)
                     /gene="dusC"
                     /locus_tag="ACHVC7_000191"
     CDS             complement(85..1032)
                     /gene="dusC"
                     /locus_tag="ACHVC7_000191"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR2114348.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1271..1669
                     /locus_tag="ACHVC7_000192"
     CDS             1271..1669
                     /locus_tag="ACHVC7_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR2114349.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1666..2361
                     /locus_tag="ACHVC7_000193"
     CDS             1666..2361
                     /locus_tag="ACHVC7_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR2114350.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2491..3375
                     /gene="cdd"
                     /locus_tag="ACHVC7_000194"
     CDS             2491..3375
                     /gene="cdd"
                     /locus_tag="ACHVC7_000194"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR2114351.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3525..4244
                     /gene="sanA"
                     /locus_tag="ACHVC7_000195"
     CDS             3525..4244
                     /gene="sanA"
                     /locus_tag="ACHVC7_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR2114352.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4247..4486
                     /locus_tag="ACHVC7_000196"
     CDS             4247..4486
                     /locus_tag="ACHVC7_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR2114353.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4680..5918
                     /gene="preT"
                     /locus_tag="ACHVC7_000197"
                     /pseudo
     CDS             4680..5918
                     /gene="preT"
                     /locus_tag="ACHVC7_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5912..7147
                     /gene="preA"
                     /locus_tag="ACHVC7_000198"
     CDS             5912..7147
                     /gene="preA"
                     /locus_tag="ACHVC7_000198"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR2114354.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7274..8284)
                     /gene="mglC"
                     /locus_tag="ACHVC7_000199"
     CDS             complement(7274..8284)
                     /gene="mglC"
                     /locus_tag="ACHVC7_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR2114355.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8300..9820)
                     /gene="mglA"
                     /locus_tag="ACHVC7_000200"
     CDS             complement(8300..9820)
                     /gene="mglA"
                     /locus_tag="ACHVC7_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR2114356.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9881..10879)
                     /gene="mglB"
                     /locus_tag="ACHVC7_000201"
     CDS             complement(9881..10879)
                     /gene="mglB"
                     /locus_tag="ACHVC7_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR2114357.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11159..12157)
                     /gene="galS"
                     /locus_tag="ACHVC7_000202"
     CDS             complement(11159..12157)
                     /gene="galS"
                     /locus_tag="ACHVC7_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR2114358.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12341..13498)
                     /gene="yeiB"
                     /locus_tag="ACHVC7_000203"
     CDS             complement(12341..13498)
                     /gene="yeiB"
                     /locus_tag="ACHVC7_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR2114359.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13515..14183)
                     /gene="folE"
                     /locus_tag="ACHVC7_000204"
     CDS             complement(13515..14183)
                     /gene="folE"
                     /locus_tag="ACHVC7_000204"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR2114360.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14441..15277
                     /gene="yeiG"
                     /locus_tag="ACHVC7_000205"
     CDS             14441..15277
                     /gene="yeiG"
                     /locus_tag="ACHVC7_000205"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR2114361.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15309..17300)
                     /gene="cirA"
                     /locus_tag="ACHVC7_000206"
     CDS             complement(15309..17300)
                     /gene="cirA"
                     /locus_tag="ACHVC7_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR2114362.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17592..19061)
                     /gene="lysP"
                     /locus_tag="ACHVC7_000207"
     CDS             complement(17592..19061)
                     /gene="lysP"
                     /locus_tag="ACHVC7_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR2114363.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19266..20147)
                     /gene="yieE"
                     /locus_tag="ACHVC7_000208"
     CDS             complement(19266..20147)
                     /gene="yieE"
                     /locus_tag="ACHVC7_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR2114364.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20246..21295
                     /locus_tag="ACHVC7_000209"
     CDS             20246..21295
                     /locus_tag="ACHVC7_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR2114365.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21369..22226
                     /gene="nfo"
                     /locus_tag="ACHVC7_000210"
     CDS             21369..22226
                     /gene="nfo"
                     /locus_tag="ACHVC7_000210"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR2114366.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22230..23318
                     /locus_tag="ACHVC7_000211"
     CDS             22230..23318
                     /locus_tag="ACHVC7_000211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR2114367.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23374..24624)
                     /locus_tag="ACHVC7_000212"
     CDS             complement(23374..24624)
                     /locus_tag="ACHVC7_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR2114368.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24724..25665)
                     /gene="rihB"
                     /locus_tag="ACHVC7_000213"
     CDS             complement(24724..25665)
                     /gene="rihB"
                     /locus_tag="ACHVC7_000213"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR2114369.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25795..26493
                     /locus_tag="ACHVC7_000214"
     CDS             25795..26493
                     /locus_tag="ACHVC7_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR2114370.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26560..27810)
                     /locus_tag="ACHVC7_000215"
     CDS             complement(26560..27810)
                     /locus_tag="ACHVC7_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR2114371.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27904..28842)
                     /gene="psuG"
                     /locus_tag="ACHVC7_000216"
     CDS             complement(27904..28842)
                     /gene="psuG"
                     /locus_tag="ACHVC7_000216"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR2114372.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28830..29771)
                     /locus_tag="ACHVC7_000217"
     CDS             complement(28830..29771)
                     /locus_tag="ACHVC7_000217"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR2114373.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="ACHVC7_000218"
     CDS             complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="ACHVC7_000218"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR2114374.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="ACHVC7_000219"
     CDS             complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="ACHVC7_000219"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR2114375.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="ACHVC7_000220"
     CDS             complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="ACHVC7_000220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR2114376.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34373..35554
                     /gene="setB"
                     /locus_tag="ACHVC7_000221"
     CDS             34373..35554
                     /gene="setB"
                     /locus_tag="ACHVC7_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR2114377.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35551..35805)
                     /locus_tag="ACHVC7_000222"
     CDS             complement(35551..35805)
                     /locus_tag="ACHVC7_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR2114378.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35960..36532
                     /gene="yeiP"
                     /locus_tag="ACHVC7_000223"
     CDS             35960..36532
                     /gene="yeiP"
                     /locus_tag="ACHVC7_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR2114379.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36755..38221
                     /locus_tag="ACHVC7_000224"
     CDS             36755..38221
                     /locus_tag="ACHVC7_000224"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR2114380.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38339..39325
                     /gene="yeiR"
                     /locus_tag="ACHVC7_000225"
     CDS             38339..39325
                     /gene="yeiR"
                     /locus_tag="ACHVC7_000225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR2114381.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39364..40077
                     /gene="lpxT"
                     /locus_tag="ACHVC7_000226"
     CDS             39364..40077
                     /gene="lpxT"
                     /locus_tag="ACHVC7_000226"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR2114382.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40489..41055
                     /gene="mepS"
                     /locus_tag="ACHVC7_000227"
     CDS             40489..41055
                     /gene="mepS"
                     /locus_tag="ACHVC7_000227"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR2114383.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41236..42792
                     /locus_tag="ACHVC7_000228"
     CDS             41236..42792
                     /locus_tag="ACHVC7_000228"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR2114384.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42874..44688
                     /locus_tag="ACHVC7_000229"
     CDS             42874..44688
                     /locus_tag="ACHVC7_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR2114385.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44689..45783
                     /locus_tag="ACHVC7_000230"
     CDS             44689..45783
                     /locus_tag="ACHVC7_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR2114386.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45783..46808
                     /locus_tag="ACHVC7_000231"
     CDS             45783..46808
                     /locus_tag="ACHVC7_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2114387.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46810..48399
                     /gene="yejF"
                     /locus_tag="ACHVC7_000232"
     CDS             46810..48399
                     /gene="yejF"
                     /locus_tag="ACHVC7_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR2114388.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48403..48747)
                     /locus_tag="ACHVC7_000233"
     CDS             complement(48403..48747)
                     /locus_tag="ACHVC7_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR2114389.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT        12011 a        12684 c        11978 g        12257 t
ORIGIN      
        1 ctcggcaatt ttgacgccac caaacaaccg tgcggcctat tgataaagag ctaacacatt
       61 gtcaaaaaac atcactatgg ttttttagag tttctcgata tcaattgcct gaatagccct
      121 tgcaatatca ggggaattat tcaacacccg aacatgctga aataattccg ttgcttcatc
      181 gtattcttta cgcaaataac tcaaccactg tttaatccgc gcaacgtgat ataacccggt
      241 atcgccctgc ttttccagac gggtatattt ttgcagcaaa gcaaccacct ccggccacgg
      301 cattcgcggt tcgttatatt ttactacccg gctcaggttg ggaatattga gcgccccgcg
      361 accaatcatc actgcgtcgc agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc
      421 ccagatttcg ccgttggcaa tcaccggaat attcagccgc tggcgaatct cgccaatcgc
      481 ctgccagtca atatgctccg cgcggtaacc ctgctctttt gtccgtccat gcaccaccag
      541 ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca aatttcttct caccgctgtc
      601 ccagcccaga cgcactttca cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc
      661 ttttgcaccc tggtagatga gttcaggatc tttgagtaac gtcgccccgc cgccgctgcc
      721 gttaaccgct ttcgacgggc agccgcaatt gagatccacg ccccaggaac ctaattccac
      781 cgcacgggcg gcgttctctg ccagccattg tgggaactga cctaacaact gaacgcgcac
      841 cagcgtaccg gatggtgtcc ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac
      901 ttttaccggc agcagttgat ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc
      961 gttaacttcg gtcagcaatt ctcgcaccag agagtcaagc acaccctcca tcggagccag
     1021 taacacacgc atatcttcac ccggaaaaaa atgaggcgct atgttagcgc ctatggtcaa
     1081 cggattaaag gtctgcaaag agggattgct gcaaactgta atccctctct atgcttgatg
     1141 tatgcctgag ttcgttgcgc attgcgtaat gccggggagc gaaaaaataa gcgctgattt
     1201 cttaatgtga tcggtagcac gttttacgaa ttaattgtat gatgaatcca tctcatctgg
     1261 ggtgttgatt atgagtaaga cactgaacat tatctggcaa tatttacgcg ctttcgtcct
     1321 gatttatgcc tgcctgtatg caggcatttt cattgcttcc ctgctaccgg taaccattcc
     1381 gggcagcatc atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc
     1441 aaaatgggtc aatccggggt gctacgtact gattcgctat atggcgctat tgtttgtgcc
     1501 gattggcgta ggcgtcatgc aatattttga tttgctccgc gcacagtttg gcccggtagt
     1561 ggtttcctgt gcagtcagta cgctggtggt ttttctggtg gtgagctgga gttcacaact
     1621 ggtacacggt gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc
     1681 tggtggtcat taccgctgac gttgattgtc ttttttgccg cccgcaaact ggcggcacgg
     1741 tataagtttc ccctgcttaa cccgttgctg gtagcaatgg tggtgatcat tcctttttta
     1801 atgctgaccg gcatctctta cgacagctac tttaaaggta gcgaagtgct aaacgatctg
     1861 ctgcaaccgg cggtcgtcgc gctggcctat cctttatatg agcagctaca ccagatccgc
     1921 gcgcgctgga aatcgatcat caccatctgt tttatcggca gcgtggttgc gatggtgacg
     1981 gggacttccg tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg
     2041 aaatcagtca ccacgcctat tgcaatggcg gttggcggca gtattggcgg tattccggca
     2101 atcagcgcag tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg ccatacattg
     2161 cttaatgcga tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg gacagcctcg
     2221 cacgccctcg gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc atttagttcg
     2281 ctggcgctgg tgttatgcgg gataattact tcgctgatcg caccgttcct tttcccgatt
     2341 attctggcag taatgggcta aaatttgcga tgcgtcgcgc atttttgatg taagtttcac
     2401 gcgttgcata attaatgaga ttcagatcac atataaagcc ataacgggtt cgtaaactgt
     2461 tatcccatta catgattatg aggcaacgcc atgcatccac gttttcaaac cgcttttgcc
     2521 caacttgcgg ataacttgca atctgcactg gaacctattc tggcagacaa gtacttcccc
     2581 gctttgttga ccggggagca agtctcatcg ctgaagagcg caacggggct ggacgaagac
     2641 gcgctggcat tcgcactact tccactggcg gcggcctgtg cgcgtacgcc attgtcgaat
     2701 tttaatgttg gcgcaattgc gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg
     2761 gaatttattg gtgcaacaat gcagcaaacc gttcatgccg aacaaagcgc gatcagccac
     2821 gcctggttga gtggtgaaaa agcgcttgca gccatcaccg ttaactatac gccttgtggt
     2881 cactgccgtc agtttatgaa tgaactgaac agcggtctgg atctgcgtat tcatctgccg
     2941 ggccgcgagg cacacgcgct gcgtgactat ctgccggatg cctttgggcc gaaagatctg
     3001 gagattaaaa cgctgctgat ggacgaacag gatcacggct atgcgctgac gggtgatgcg
     3061 ctttctcagg cagcgattgc ggcggcaaac cgttcgcaca tgccttacag taagtcgcca
     3121 agcggtgtcg cgctggaatg taaagacggt cgtattttca gcggcagcta cgctgaaaac
     3181 gccgcattca acccgactct gccaccgttg cagggggcgt taattctgtt gaatctcaag
     3241 ggttatgatt acccggatat ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg
     3301 attcagtggg atgccacctc cgcaacgctg aaagctctcg gctgtcacag tatcgaccgc
     3361 gtgcttctcg cttaagcctg gtgccggatg cggcgtgaac gccttgtccg gcttgccagc
     3421 cctctcctgg tgtcgaaatt cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg
     3481 gtaaagtagc ctgatggaaa ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt
     3541 tcctcagcct gttagtcctg atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct
     3601 ggatgagctg gaaaaccgcg ccttatatct acgacgaatt gcaggatctc ccctaccgcc
     3661 aggtcggcgt ggtgctcggc acagcaaaat attatcgtac tggcgtaatt aatcagtatt
     3721 atcgctaccg cattcaagga gcgattaatg cctataacag cggtaaggta aattatctat
     3781 tactgagcgg cgataacgca ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt
     3841 taatcgctgc tggtgtcgac ccatcagata ttgttctcga ttacgcaggc tttcgtacgc
     3901 tggattccat cgtgcgtaca cgcaaagtgt tcgatactaa tgatttcatt attatcaccc
     3961 aacgtttcca ctgtgagcgg gcattattta ttgcgctgca tatggggatt caggctcagt
     4021 gttatgccgt accgtcaccg aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc
     4081 gtttcggtgc gctggctgac ctttatattt ttagacgtga accgcgtttt ttagggccgc
     4141 tggtccctat tccggctatg caccaggtgc cggaagatgc gcaggggtat cccgccgtca
     4201 cacccgaaca gttacttgaa ttacaaaaga aacaaggaaa gtagttatgg atgtacagca
     4261 gttttttgtc gttgccgttt ttttccttat cccgatattt tgtttccgcg aagcatggaa
     4321 aggctggcgc gcaggcgcga ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt
     4381 ctggcgagca aaaaatcccg gactcttttt cgcttatatg gtggcatata tcggcttcgg
     4441 aattttatct atcggcatga ttgtttatct tattttctat cgttaatatt ttctcttttc
     4501 tgttaattat tgagaataat tattacttca ctcaataagc tgcggatata acattctgaa
     4561 ccgcagctat ttgtgaatcc tttcacagtt taaatttccc cacacgctta gccttaatat
     4621 cagcacgcaa tatttattta gcgttcgcct taattactca tagcattaag gaagatcaca
     4681 tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccttta ctggcgatta
     4741 aagaagcctc tcgctgttta ttatgtcatg acgctccctg tagtcaggct tgcccggcac
     4801 agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg
     4861 agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga
     4921 aattatgcca aagcggttgt acccgtgccg gtgttgatgc tcccattgat atcggccgct
     4981 tacagcgttt tgttactgat tttgaacaac aaaccgggat ggaaatttat cagcccggta
     5041 ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg
     5101 tgacactgac aaaccagggt tatgacgtca cgatctatga gaaagaagca caacccggtg
     5161 gctgattgcg taacggcatt ccgcaattcc ggttaccgca atcagtactg gatgcagaga
     5221 tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac
     5281 tcacccttga gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcaa
     5341 gcggttccgg gctaccgctg tttgagcata gtgacgttga gattgccgtc gacttcttgc
     5401 aacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg
     5461 gcggtgatgt cgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa
     5521 cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttaccagcg
     5581 cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata
     5641 aagtcacgtt taagcatgta cggttatcgg gcgaactgac gatggcggca gataaaatta
     5701 ttctcgccgt cggtcagcat gccagactag atgcctttgc ggagttagag ccgcagcgta
     5761 acaccatcaa aacacaaaat taccagaccc gcgacccgca agtctttgct gctggcgata
     5821 ttgttgaggg agacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg
     5881 cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt
     5941 ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgccggtag gcaactgcta
     6001 tgagatgtgt gccaaagcct acgacacagg ttggggcggt gtggtgttta aaacgatcgg
     6061 cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg
     6121 ttttatcggc ttcaaaaata tggagcagat tgccgagcat ccgctggagg agaatctggc
     6181 cgccctgcgt cggctgaaag aagattaccc ggacaaagta ttgatcgctt cgatcatggg
     6241 ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat
     6301 gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg
     6361 gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc
     6421 aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa
     6481 gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga
     6541 tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcaagtattt ccggatattc
     6601 cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca cccatccaga
     6661 actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggcgga
     6721 gtttttattg ctcggcgcag caacgttaca ggtcaccacc ggcattatgc agtacggcta
     6781 tcgcatcgta gaagatatgg cgagcgggtt atcgcattat ctcgccgatc agggatttga
     6841 ttcgttgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga
     6901 ccgcagttat attgtctatc cccgtatcaa tcttgacaaa tgtgttggct gtggacgctg
     6961 ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac
     7021 accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt
     7081 gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac
     7141 gttgtagaac ccgcgtcaca tccggcattt ggttcctgtt gccggatgcg gcgtgaacgc
     7201 cttatccggc ctacaaaacc acacagatca tgatgttatg tgcgtttatg accgaatgcg
     7261 gaccacattc acatcatttc ttacgcgcgt atttcagtga atccagcgct acggcgaaga
     7321 taataatcgc ccctttgatg atgtactgcc agtatgggtt tacgccgata tacgtcaggc
     7381 catagttgat gacggtaaaa ataatcaccc cggtcaccac gccaatcacc gtccccacac
     7441 cgccgctgaa cgatacaccg cctaccacgc acgccgcgat agcatccagc tcatacataa
     7501 agccgaggtt gttggtggca gagccgatac gtccggcttc taacatcccg ccaaaggcat
     7561 agaacacgcc agacaacgcg tagatcatca gcaggttcag gccgacgttg acaccagata
     7621 cttttgccgc ttccgggtta ccgccaatgg caaaaatgtt cttaccgaag cgggttttgt
     7681 tccacaacac ccagacgaac gccaccgcaa tcaacgcgta gaaggtgatg taagagagac
     7741 ggaaactccc cagcgcgaca aagccctgag caaaggtaga gaagccactg tcaaaaccag
     7801 aaattggcga cgccccgaca aagtcgtaat agagcgagtt gatgccatag acgatgatca
     7861 tcgtgccgag cgtggtaatg aacggcgtca cgttgagata agcgataatc agaccgttga
     7921 tcaaaccgat caccgcacca atggcacaga caatcagaat aaccagcgca atcggcatcg
     7981 tcgccatttc cgggaacact ttgttggcgt tatccatgga ctgcaataat gtcgcagcca
     8041 ccactgccgc cagccctacc tgacgaccag cagaaagatc ggtcccctgg gtgacaatta
     8101 accctgccac accgagcgcg ataataatac gcaccgatga ctgggtgaga atattactta
     8161 agttcaacag acttaaaaat gttgggtcct ggaaaataat aatcgccagc aaaactaaaa
     8221 gaacgacgta aataccgccc tctttcaggt aagtaagaaa acttttctta tttaacgcac
     8281 tcatgggaag cccctaatct taaaggtgca aagacgcaag acgcagaatt tcgttttgcg
     8341 ttgttgtttt tgtatcgaca attccggaaa cgagaccatt gctcatgacc agaatacggt
     8401 ctgttatccc taacaactca ggcatttcag aggagataat aataatcccc ttgcctttct
     8461 tcgccagttc ggcaattaac tgataaattt caaacttcgc cccgacatca ataccgcgcg
     8521 tcggttcatc gagcattaat atttctggtt gcgttagtag ccagcgacca ataatcacct
     8581 tttgctgatt accaccggag agcgaaccaa tttgcgtccg atgacccggc gtttttaccc
     8641 gcatcgaatc aatcacccac tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa
     8701 ctttattttt gtagttgcga atattggaaa ttaacgagtt aaaaccaata tccagatagg
     8761 cataaattcc cgttgagcgg cgctcctcag ttaccagtgc aaatccgtgg tttatggctt
     8821 cgttggcatt atggttattg atctgtttgc cgtgcaacgt aatggtgcca gccgatttct
     8881 cgcgaatacc aaataacgtc tcaacaatat cggtacgttt cgcccccacc agcccggcaa
     8941 taccgaggat ctccccttta tgcagatcaa acgagacatc gcgaatcgac ggctggcgca
     9001 gtgacgttag gttacgcacc tcgaggatga cttcgcccgg cttgttttct ttgtcaggaa
     9061 aacgctgatt aagagaacgc ccaaccatca tggcgatgat cttgtccatc gtcagtcctg
     9121 ccagcggttc ggtggcgatc cactgaccgt cgcgcaatac ggtaacttca tcacataact
     9181 ggaagatttc ttccatttta tgcgagatat aaacaatacc gcagccgcgc tcttttaatt
     9241 tacgaataat agtgaacaga tgattgacct ctttttcggt taacgaagaa gtcggttcat
     9301 ccataatcac aattttcgcg ttataggaaa acgctttggc gatttcgatc atctgcattt
     9361 gcgaaacgga taatgtgccg acgcgcgcac gcggatcgat atcaatatcc agttcatcaa
     9421 aaatcgcttt ggtttcgcgg tacattttgt cctgatcgac aaacatgcct ttggtgggat
     9481 atcgccccag ccacatgtta tccatcaccg aacgttgtaa taccaggttt aactcctggt
     9541 gtaccatcga aataccattt tccagtgcct ctttggcgga atggaaatcg atctctttac
     9601 cctggaataa aatggtgccg gagtcttttt gataaatacc aaacaggcat tttaataatg
     9661 tcgattttcc tgcaccgttt tcccccatta atgcatggat agaatgtggc cggaatttta
     9721 aattaacgtt atcaagtgcc ttaacaccag gaaaggactt gttgataccg ctcatttcca
     9781 acaagtattc cccggaagac ggagtcgttg agctgaccat ataattttac cttgttggcc
     9841 atacaataag ggcgcagtaa acgactgcgc ccaatcagtc ttatttcttg ctgaattcag
     9901 ccaggttgtc tttatctacg ccaacataag gtacgcggac cactttgttg tcgattttcc
     9961 agttggtgcc atcagccgca cctttgccat cggccaggtt tttcgccaga tcaaaggtcg
    10021 ctttcgcctg gttgttagca tcgttcagta ctgtgcccgc cagtgcaccg gatttcacca
    10081 gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg
    10141 ctttcagtgc ttctaccgcg cccattgcca tcgcatcgtt gttggcgata accacttcga
    10201 ttttgttggc gttcgggcca gacagccagg cgtccatctt atctttcgcc tgagcggtgt
    10261 cccacattgc ggtatctaac tgtaactgtt cagttttgat gcctttatcg ttcaattctt
    10321 taatcacgta agtggtacgt gcttctgcat ccggatggcc cggttcacct ttcagcagta
    10381 cgaactgaat ctgaccgtct ttgttcagat cccaaccctg attcgccgcc cagtgtttag
    10441 caatcaaatc gccctgaata atgccggact ctttggagtc agtgccaacg tagtaggctt
    10501 tgtcgtagct atccagcgcc ttacgagacg gttctttgtt gaagaaaacc accggcacgt
    10561 tttgcccacg cgctttctca atcaccgtac ccgcagctgc cgggtcaacc aggttgattg
    10621 ccagtgcctt cacccctttc gccagcaata cgtcgatctg atcgttctgc ttggactggt
    10681 cattctgaga atcattcatc agcagctgaa catctggcgc ggctttcgca tcttgctcaa
    10741 tagccttgcg cactacagac ataaagttat cgtcgtactt atagattgtt acaccaatgc
    10801 gagtatcagc agcgtgtgca gcggcaccga ataacatgct ggccatcaca gcagacaggg
    10861 ttaacacctt cttattcatg gtatctccgg tttttcttat gcagggtagt gcttgagata
    10921 aatgctcggc ggggcagtag agttaatgaa gtgttactga acgccgaagc tcactttttt
    10981 aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg
    11041 ctattacctc ggtgaaagtg attattcact gttacatacg ggttacaacg ttaaaacggt
    11101 gcaatcatag ctatcacatt gttaagatac tgtgaaatca ctcacagatt gaaagcggtt
    11161 acatcgcctg atttgttgag ttagtgatcg ccgccgcatt ctggcgcgtt gcgacagaat
    11221 ggcgacgcac taacgtcggc ataaaacagt ggctggcacg aggatcaata ttgcctgctg
    11281 ccccctgcaa ggccagttcg gtggctaatt tagccattga agcaatggga taacgcacgg
    11341 tcgttaattg cgggtcggta taacgggcga tgggaatatc atcgaaaccg atgattgaga
    11401 gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt cagtgcgcca gcggccatat
    11461 tgtcgttata agcaaatata gcggtaagtt gtagattgcg ccccagcagt tcaaccatcg
    11521 ccgcctcacc gcccggcatg tccggcgtac cagtgccaat ccagctttcc ggcggaataa
    11581 tatcctgctc tttcaacgca ctcatccagc ctgctttacg catggcgtca tcttcaatgc
    11641 cgtgactgga agaaagataa ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg
    11701 ccattcgggc accgctgaga ttatccaggc aaacgcaacg atgggcgtac cccggcacaa
    11761 cgcggttgat taacaccata ccgggaatgt tatccataaa ttgcgccagt tcatcgtcac
    11821 tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg gcgaattaac acctcaatgg
    11881 cgtgacgctc tttttccgct tcatgatagc tattgccgat tagcacgtat ttctgatgct
    11941 gctgagcgac tagatccacc gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca
    12001 tcaccaccac gccaatggtg tcgctaacct gagttgccag cgcctgggca ttggcgtttg
    12061 gccgataatc cagctcactc acggctttca ttactgcttc acgcgtgtcg gcactgacca
    12121 gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga gacgccagcc taacgcgcta
    12181 catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat gactgcttgc
    12241 tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac agcaatggaa
    12301 acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat gttttagata
    12361 ttgccgttcc ggcagcacgc aaagttaact gacgccagag ccattccacc ggcccctggc
    12421 ggaagtaacg cagccagata acagagaaga ggatattcgc cagccatacc ggaataacaa
    12481 acgccagcag ctccaggcgg tcaaaatgca taaacaaacc gaggtggtaa aaaagcatgg
    12541 tacaaatcag cgtttgcaat agatagttgg tcagcgccat ccgtccgacg caggcgatcg
    12601 caagcaccag cttaaagcgg ctcaattgcg gccagaagcc ataaaacagc gacgcatagc
    12661 caatcgcctg aaacggcgca ctcagttccc gcggcatttg aagtaagaag gcacaccagc
    12721 gatacgccca gtccagctgc cattgcaggg cgatggcagg aaggttaatg gtcaccccaa
    12781 tcgccaccag cacaaaacca gtacgacgat agtgacgtaa gctgaactgc cctttcagcc
    12841 agccgctgcg catcagcgcc gcaccaatga gcatcatccc tgccagttgc cagccatact
    12901 gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc acgattactg atcgcttcaa
    12961 cgccgccgtg aagcttccag tatttttcat acaaaatagc cgatgcatcc ggcgtccagg
    13021 tgcggctggt ctggctgtcg gaaatcagcc ccaataacag caaaacgcca agccccacca
    13081 gataaagcat gacgcctgta ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc
    13141 gccagcagat taagcccacc agcccgtaag ccagcagaat atcgccgtcc cagaacaata
    13201 aaccgtgaat aaaacccagc aagactaaca gcgttaaccg cgactggatc cagcgtctgc
    13261 cacggggcag caacatttgc aggcccgcac caaacagcag cgcaaaaagc gtgaggaatt
    13321 tcacctggcc gatgagatcg agaaatgccc aggtccatgc atcctgcggc gtaatagcgc
    13381 cgtaccaggc gggattgaga taagccgcct ttggtagccc aaaggcgctg atgtttaata
    13441 gcaggatccc cagaatggcg acgccgcgaa caaaatcgag cgttacgttg cgctccatgg
    13501 ttcctgcctt ttaatcagtt gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc
    13561 tgactggatt tgaacaatcc accaagagag gtcgttgttg tggcactggt tgcatcgcgg
    13621 atgccacgcg ctttcacgca gtaatgcacc gcatcaatgg agacagccac gttattggtg
    13681 cccagcagcg tttgtagcgc aataagaatt tgctgcgtca gacgttcctg cacctgcgga
    13741 cgctgggcaa agaactgcac gatacggtta atttttgaca gaccgatcac cgaatctttc
    13801 gggatataag ccaccgtcgc tttgccatcg atggtaacaa aatggtgttc acaggtactg
    13861 gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg
    13921 gtgattttcg ggaagttggc gtaatccaga ccggagaaaa tttcatcgac atacatttta
    13981 gcgatgcgat gcggcgtttc catcaaactg tcatcagcca ggtcgagatt cagcagctgc
    14041 atgatttcgg tcatatgacc agcaataagg cttttgcgcg tttcgttatc catttcatgc
    14101 acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta acgcttcatg aaccagggcc
    14161 gcttctttac tgagtgatgg catttatgat ttctcctgca ggtgtgacgc ctccgccctg
    14221 cgtgggggca aagttattaa gctgatttac agcctgatta ttgtgcgtga ggcggcgcac
    14281 ataatccagt attcacagcg ataattattg taattgccgc tgactttcat cagcagatgt
    14341 taaaacatcg ttatgcaaat acggaagtga aagttactca cagcacattg aataaacggt
    14401 atgatgaaga aattgcaaac aacacaacaa ggagccacgc atggaaatgc tcgaagagca
    14461 ccgctgtttt gaaggctggc agcaacgctg gcgacacgac tccagtacct taaactgccc
    14521 gatgacgttc agtatctttc tcccaccccc tcgtgatcac accccgccac cagtgctgta
    14581 ctggctttcc ggattaacct gcaatgacga gaacttcacc accaaggcgg gtgcccagcg
    14641 cgtggcggca gaactgggga ttgtactggt gatgccagac accagcccgc gcggcgaaaa
    14701 ggttgccaac gacgatggct acgatttagg ccagggcgca ggcttttatc ttaatgccac
    14761 gcaaccgccg tgggcgacgc attaccggat gtatgattat ctgcgcgatg aattaccggc
    14821 gctggttcag tcgcaattta atgtcagcga ccgctgcgcc attagcggtc actcaatggg
    14881 tggtcacggt gcgctgatta tggcgctgaa aaatccgggt aaatacacca gcgtttcggc
    14941 ctttgcgcca attgtgaatc cgtgcagcgt cccgtgggga atcaaagcgt ttagcaccta
    15001 tttaggtgag gacaaaaatg catggctgga atgggatagt tgcgcgctga tgtatgccag
    15061 taacgcgcag gatgcgatcc cgacgcttat cgatcagggc gataatgatc agtttcttgc
    15121 cgaccagttg caacctgcgg tattggcaga agccgcgcgc cagaaagcgt ggccgatgac
    15181 gctgcgtatt cagccgggat atgatcacag ttactacttc atcgcctctt ttatagagga
    15241 tcacctgcgc ttccatgcgc agtatttact gaagtgaaag tccgcccggt tcgccgggca
    15301 tcttctcatc agaagcgata atccactgcc ataaagtaac gacgtccgtc ttcgttatag
    15361 ctgtagtcgt cacgactgag atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg
    15421 acgtctttag tcacctgcca ggccgcgccg gtattccaga tggtataacc gcccggtgtt
    15481 ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt gaccagaaac atagaatgac
    15541 cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt tagcagtatg gaacggcaga
    15601 tcggatagcg gtttgttttc gccgttgctg acatcacgac catcgttgta ggtgtagttg
    15661 atcgacagtt tccattcatc gttgaacgga attttcagtt cggtttccac gccctgaata
    15721 cgagctttgt taacgttgta gtagctaaat accggtatgc gccgtccgtt agcgcccgtc
    15781 tcaaaaccaa caaagttttg gtagcccggt gcagcgttga cgtcagacgt acggctgatg
    15841 ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac cccttccagc
    15901 cagccttctt cacccatgta gtaaagcccc agctcccaac tttcgctggt ttctggtttc
    15961 agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt ccagtcaggg
    16021 ctaagttgca acagagaagg tgctttaaat gccgtcgccc agcccccttt caccgttacg
    16081 gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc gtaggtttcg
    16141 tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct cgaagatccg ccattcatct
    16201 tccacaaaca gcgcgtactg gctggcagac gttttggagc tggttccccc ggtcaggttc
    16261 accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc
    16321 gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt tatcgggctg
    16381 ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc gctggtgccg
    16441 taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg gtttttgtcc
    16501 agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt
    16561 tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc
    16621 ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt catccttttc acgttttgcc
    16681 aggctgccgt aagctttcat tcccagcaca ccatcaatta atggtccact ggtaaagaac
    16741 tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa tggtggtatc gacggtaacg
    16801 gtacccgacc atttctgacc gatttttttg gtgatgatat tcactacacc gccgagcgca
    16861 tcggaaccgt acagcgacga catcgggcca cggaccactt caatacgttc gatggaatcg
    16921 accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg ggagttcacg
    16981 cgtttaccgt cgacgagaat cagggtatag ctgctgtcca gaccacgaat actaacgccc
    17041 ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc
    17101 agattctgta ccggttttcg ctgcagatct tcctgggtaa tgacgctgat actggcgggt
    17161 gcatctttaa ggttttgttc aacggaagat gcagtgacaa ccatcgtttc gccatcgtca
    17221 tcgaccgcta acacaggcca tacacaagaa atagcggaca aacacagccc gacccgtacg
    17281 aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggta
    17341 tcgctcacat cttctttacg tccccttgcg tacggcagca tcgcggtgga cttatttgta
    17401 tgcaggtgat catcccagaa agccggacca gcctctgatt tgtcagcctt ttttgattgt
    17461 agcgtaacga taatgcaaac gataacagtt atcaatccaa atgttaaatt ttatatccgc
    17521 atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc ttagcaagga
    17581 agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta gcgtacgaag
    17641 tgagttcctt taatcagctt gtagccgaac caaataatca ggaacagcgg gataccaata
    17701 tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc gtagttctgg
    17761 cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccagtgg gaagaaacct
    17821 gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga
    17881 tagtggctaa tggcaatccc cagccaggcg ataaaacccg tcatcccgga ggtgttcagc
    17941 agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacaa accggcaatc
    18001 accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacaattt agcgaaaatg
    18061 cgcggcgctt taccgtcaca cgccagggtg tacagcatac gagtagacgc atacataccg
    18121 gagttacctg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag
    18181 agcagacccg cgtgctggaa caccaaggtg aacggactga cgctgatgtc tttaacatcg
    18241 ttacgcagca ggctcggatc ggtatacgga ataatcaggc tgataatcag gatcgcgaac
    18301 acatagaaca acaggattcg ccagaatacc tgacgtaccg cgcgtggaat gtttttcgcc
    18361 ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa agagaagccg
    18421 acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc ttcgccgatt
    18481 gtccagttgc tccagcccgc aggctgcgcg cctttgaaga taccgataat catcagcacg
    18541 ccaacgatga taaagacaat aactgtcgtg actttgatca gtgaaaacca gtattccgct
    18601 tcaccaaagc cacgaactga gatgtagttc agcaggaaga taacgccgag gaacaacgca
    18661 ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg tgctgcaacc
    18721 aagtcaacgg caatagtcac cgcccagttg taccagtagt tccagcccag cgcgaagcca
    18781 aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat
    18841 gcagccaatt caccgagact ggtcatcagg aagtaaacca tcaggccaat cagcatatac
    18901 gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc aacaaaaaga
    18961 cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt taattcacgg
    19021 cgtaagcccg gcgcttctgt agttttagtt tcggaaccca tacaaaaatg ctatccatct
    19081 taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa atacacggtt
    19141 ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct cttttctggc
    19201 gaatgcatgc tgagggcgct gcgtcacgaa atcgacgcag caccagcatg ttcttgtaca
    19261 gcaccttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg
    19321 gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg cgggagaggg
    19381 accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacacg tcgcgacagg
    19441 caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt acctaattcc
    19501 atcgccatct caaacttcgg taaatgtgac agcaacagat aatcgacaat ctcccgcgtg
    19561 ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgttctaa ggtgaccgga
    19621 ccacgagcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc cagccacggt
    19681 tcagaaatga tttcagtgct gtggcacggt ccttcaataa agccaatatc aacgcggaaa
    19741 tccagcaccg cttgcatcac gtcctggctg ttcccaacgc taagttcaat cggcaactgc
    19801 ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc gatggtacta
    19861 ctggcataga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc
    19921 tgttcaagca atgccaacgc acgcggatag agcagccgcc cgtgttcatt aacaaccagt
    19981 cttttcccca cgcgatcaaa cagttgcacg ccaagctgcc cttccaggtc ggtcaaggct
    20041 gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt tgatccactt
    20101 ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt cgcttaccac
    20161 ttataaagat taattataaa tatataatca attttatttt taaaccagtt agtcgtaacc
    20221 ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac aacatcgtac
    20281 actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg tcgccctgtg
    20341 gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc tcgcaatctt
    20401 gttggggatg gttttaggca acaccatcta tccgcacatc tggaaaagct gtgacggtgg
    20461 tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg gcttccgtct
    20521 gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg tgttgacgct
    20581 gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc tggataagca
    20641 caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg tactggcgac
    20701 tgagtcggta gtgaaagcgg aagccagtaa agtaaccgtg gctgttgcaa ccgttgttat
    20761 cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt ctcaatggtt
    20821 tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg cgcaggtggt
    20881 ggcggcaggt catgccatca gcccggatgc ggaaaacgca gcagttattt ccaaaatgct
    20941 gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta aacagctgtc
    21001 tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca tcttgttcat
    21061 cgtcgttgcc atctttaact cgttccacct gttaccgcag agcgtggtga acatgctggt
    21121 aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga ccactcacgt
    21181 cagcgcgctg aaaaaagctg gggcgaaacc tctgctgatg gcactggtac tgtttgcctg
    21241 gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg cataaaccac
    21301 tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg tttaacagga
    21361 gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg gcaaatgccg
    21421 caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa aaccaacgtc
    21481 agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc gcctgtgaaa
    21541 aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt aacctcggac
    21601 atccggtcac tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa atgcagcgtt
    21661 gcgaacaact ggggctttct ttgctcaact tccaccctgg cagccatctg atgcagattt
    21721 cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgcgctg gataaaactc
    21781 aaggtgtaac agcggtgatt gaaaacaccg ccggtcaggg cagtaactta gggtttaaat
    21841 tcgaacatct cgcggcgatt atcgacggcg tggaagataa atcccgcgtc ggcgtctgca
    21901 ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactccagcc gaatgcgaaa
    21961 aaacgttcgc tgattttgcc cgtattgtcg gctttaagta tctgcgcggg atgcacctta
    22021 acgatgcgaa aagtaccttt ggcagccgcg ttgaccgcca tcatagcctc ggtgaaggca
    22081 atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac ggcattccgc
    22141 tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg ctgaaagcgc
    22201 aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag attcttaaaa
    22261 ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg caaatcagcc
    22321 gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt aaaggcaaag
    22381 gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat atggatattc
    22441 gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct gttcactgtt
    22501 ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt cgggatgtgc
    22561 atttaatatc agctattggc aacgattttt acggcgagac attactggaa gaaacgcgcc
    22621 gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact gcaacttatc
    22681 tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca catattctgc
    22741 aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat gctggtgtgg
    22801 tgctggctga ttgtaatctc acacctgaag ccctggagtg ggtctttacc attgctgatg
    22861 aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta aaaaactggt
    22921 acagccggat ccacactctg aaaccaacac aaaatgaact ggaaattttg tgggggcaac
    22981 cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa caaggtgtta
    23041 aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag gatggagaac
    23101 aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    23161 ttatggcggg cctggtatat agctttcttg aaggatacag tttccgcgac agcgcccgtt
    23221 ttgcggtagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac cctaccctgt
    23281 ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct
    23341 tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa agaatcctgc
    23401 aatagtagca ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta aaccaagttg
    23461 ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaac gcccccacca ccaccccgat
    23521 agaaccaaag ttagcgaaac cgcacaacgc gaaagaaata atcgccacgg ttttagcatc
    23581 cagagtgcca cccgtttgca gatatggcga gaaattgaga taagcgacaa attcattgat
    23641 cgccagcttc tgcccaatca aactcccggc aagatttgca tcactccagt caacccccat
    23701 cacccacgcc aatggggcca acaggtaacc taaaatcgac tccagcgaag catgttcaaa
    23761 accaaaccag ccgccaacac cgccgataat accgttaatc aacgcaatta ttgcaacaaa
    23821 tgccataaca actgtcgcta caccggcggc gattttcagc ccggtcattg cccctgtcgc
    23881 agcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat taaaagaaac
    23941 ctgtgaagat tccgtagccg ggcttaacag gcgggcaaac aagatcccgc cagggatcgc
    24001 cattaacgat gccgccagca aatattccac aggtacgccc agtgcggcgt aaccaatcat
    24061 tgtcgaacca gcaatcgagg ccatgccact acaaatcgct gtaaataatt cattgcgatt
    24121 cagacgatcg ataaagggtt tgacgatcgc cggaatttcg ttttgcccga ggaaaatggt
    24181 ggtgaccgcg acgaatgact cgatcttgct gatatttaaa gctttctgga agataccgcc
    24241 gagaatgcga attaagatcc ccatcacacc gatgtagtag agaatactca ccagtgcagt
    24301 gacgaagata attgccggta ataccctgaa accaaagata aatcctgcgc catcaaataa
    24361 ggtatccatt ttcggtccga ccagagaacc gaagataaat gcgctacctg cgtcgctgta
    24421 cgccatcact ttgtggacgc caaaagcgac tttttctgcg acccaacgcc ctggcggtaa
    24481 ccaaagcata atcccgccaa tcacgacctg taacactaac gccgcgccaa cggtacgcag
    24541 gctgatcttc ttcttgttta ctgacagtaa aaacgcaatt gccagcagta ccaccattcc
    24601 cagaacactt ctcatgacat ccatagtgat tatctcttca tgtcattttg ggcgacggct
    24661 ttctgcccat cagaaatagc cgggcctgca tcttgcgata ctgtcccggc ttgttatcaa
    24721 cacttaatgg gtttttatgt agccgcgtac gcactcttcg actaatcccc agaaccagtc
    24781 ggtatcaata gtgatgccga ctttagtatt ggcgggcttg ccaagaacgc ccagctcgtc
    24841 gcagacggta cgcccatagc aagggccact gttgacgtcc acttcgacgt acatctcctg
    24901 ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcgt gcaccgggcc
    24961 gccagccagg ccgtagtttt cgaactgcgt tttgagagtg aagttcatga tgtcgctgaa
    25021 catctctccg gcagggccgc ctgccctttc cattcgtgca attacgtccg gggtgcaaac
    25081 ggtctggttg gtaagatcga ggcccatcat cactaatgga acgccggagg tgaacaccac
    25141 gcgtgcggct tccgggtcgg caaagatatt gaactcggca gatggggtga agttgcctgt
    25201 accgtaagcg ccgcccatta gcacaatttc acggattttg ggcaggattg cgggttgcat
    25261 acgcattgcc accgcgatat ttgaaagcgg accaaccggc accagagtga tatcgccatc
    25321 gcttgccata agggtatcaa tgatgtattt caccgcgtgg gtactttctg cctggcgggt
    25381 cagcggctcg aataccgggc catccagtcc ggtttcaccg tgaatattat cggcaacgat
    25441 ttgttgacgc ataatgggtt gcggcatccc cgcataaacc ggaacattaa tctccagttt
    25501 ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac ccgcaacaat
    25561 ggtgatacct aataaatcaa tcgccggatg tttcgccgcc atcattatag caatagcatc
    25621 atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt atttcctctg
    25681 tttccagttg cgttattttt tctacagcaa cgaaatttcg atcgggccga tgattgaatc
    25741 ttaacaacag cgtacgaatg ctaaatatga gaaatctcat agcggataaa catcgtgaaa
    25801 gaaatccaca ataatgatct taagcagcaa ttgatgagtg aatccgcgtt taaggattgc
    25861 tttttaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt
    25921 gtgcaggaag ggcaacagcc gtcctggctg ttttacctga cgcgaggccg cgccaggctt
    25981 tacgccacgc tagctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc cccctgtttt
    26041 attggcgaga ttgagttaat cgacaaagac catgaaccgc gtgcggtgca ggctattgaa
    26101 gagtgttggt gccttgcgct ccctatgaaa cattaccgcc cgctgttatt aaacgacacg
    26161 ctatttttac gaaaactctg cgtcacctta agtcataaaa attatcgtaa tattgtttct
    26221 ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcatttat attactctcg
    26281 caggaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt aggcgtttct
    26341 tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt aattaaaagc
    26401 aagaaagggt atctaattaa aaacagaaag cagttgtcag gactggcgct ggagatggac
    26461 ccggagaata aattctccgg gatgatgcag taaaaattat tggcaatagc acgattgccg
    26521 gatgcaacgc ttaataaggc gtacatccgg caatgaaact caagctaaac caataaagaa
    26581 cccggcaatg gtcgcgctca tcaggttgga aagcgtcgcc gccgccagcg cccgtaaacc
    26641 aagctgggcg atttccggcg cgcgttttgg cgaaatagcc gaaaacgctc caacgacaac
    26701 accgatagaa ccaaagtttg caaacccaca gagggcgaac gagataatgg caatggtctt
    26761 gacatccagc gtgccgctcg tttgcagata gggcgagaaa ttgagataag caacaaactc
    26821 attaatcgcc agtttctgcc caatcaaact tccggcaaga tttgcatcac tccagtccac
    26881 ccccataatc catgccaacg gggccagcac atagccgaaa atgctttcca gagagacatt
    26941 ggcaaaacca aaccagccgc caactccacc aataatgccg ttgatcagcg caatgatcgc
    27001 tacaaatgcc attaccaccg tcgccacacc ggcggcgatt ttcagcccgg tcattgcacc
    27061 gttcgccgca gcttcaataa tgctttttgg cggtgtttcg ctaaacgaca gattttcaaa
    27121 cgtaacttgc gaaggttccg tcgccggact aagaatacgt gcaaacagga taccgccagg
    27181 aatagccatc agcgaagcgg ctaacaaata gtcaatcggc acgcccattc cggcatagcc
    27241 aatcatcatc gaacccgcaa tagacgccat gccgctacaa atagccgtaa atagctcgtt
    27301 gcgattcatg cgatcaataa aaggtttaac gatcgccggg atctcatttt gcccgaggaa
    27361 aatagtggtg actgcgacaa aagattcgat tttgctgatg ttgagggctt tctgaaaaat
    27421 actgccaaga atacgaatca gcagccccat tacgccaatg tagtacagca agctgataag
    27481 cgcggtaacg aaaataatcg ccgggagtac gcgaaaggcg aagataaagc ccgcaccgtc
    27541 aaacagaaca tccattttgg gaccgaccag cgaaccaaaa ataaaggcac ttcctgcgtc
    27601 actgtaggac atcactttat ggacgcctaa tgccgcctgc tctactgccc attttcccgg
    27661 gggaaaatag agcatgatgc caccaatggc aatttgcagc agcagcgcag cgccaaccgt
    27721 gcgcaagctg atgctctttt tatttaccga caacacaaag gcaattgcca gcaataccac
    27781 catccccgca acacttctca ttatatccat aatgattttc ccttcatgcc agtaaacccg
    27841 gcgtcagcgc caggttttgg tatgtttgat gagtacgggc gacggctttc tgcccgccag
    27901 aaattaaccc acgagacgct gatattcttt ggcaatttcg ctcgccagaa tggcgttatt
    27961 aaagaccagt tgaatattgg atttcaggct atcaccgccg gttagctccg caacacgcgc
    28021 cagcaggaac ggtgtactct ctttcccaat aacaccctgt tcttcggctt cagcaacggc
    28081 ttgatcaatt gctgcattga tggtttcttc tggcatcgca aactgttccg ggatcgggtt
    28141 cgctaccacc aggccgccgt tcaaaccggt ttgccacttc actgccatcg cgcgagcaat
    28201 ttcgcgggca ctgtcaagac gaatgctgac ttcaaatggg ctggttcggc agaaaaacgc
    28261 aggcagcgct tttgtctgat aaccaattaa cggtacaccg aaggtttcta aatattctgt
    28321 tgttaatcct aaatcgagaa tagattttgc ccccgcacaa acaacagtga cattggtatt
    28381 tgccagttcc tgtaaatcgg cagaaatatc gaaggtatgt tccgctccgc gatgcacacc
    28441 accaatacct ccggttgcaa aaactttaat cccagccagt gctgcaataa tcatggttga
    28501 tgcaacggta gtcgcgccat ttttccctgc agcaacgaca aaaggtaaat cacgacgact
    28561 aactttcgtc acgttatgtc cttcacggcc cagcaattcg atttcttctt tacttaagcc
    28621 cactttcatc acaccgccaa taatggcgat agtggctggt actgcgccct gtttacgaat
    28681 tgtttcttct acttcaattg cggtttgggc attttgtggg aatggcattc cgtgagaaat
    28741 tatggtcgat tccagcgcaa caaccggttt tttatttttt aaagcatcct gtacttcagc
    28801 ggatatttgt aataattcag gggacagagt taattcagac attctgtgtt ctccactaac
    28861 gatctaacat tggcaattga taattcaggg ttgttggtat attcacaggc aagtgccatt
    28921 gacgaacatc cctgcgcaaa tcgaacagaa tcgataaacg gcataccgtc tacccaacag
    28981 gaggccagcc ccgccatcat ggcgtcgcct gccccagtaa cattgatgac attcgtttta
    29041 ataggggccg accacccact ttcaccgttg atatcactgt agtaaacgcc atcccctccc
    29101 atgctcagca ccaagcggtt caggccgtgt tgatgaaacc aggcggcgac ctttgcgaca
    29161 tcttcacgtc cggaaagtgc aattccgctc aacgtttcgg cttcaagacg gttaggtttt
    29221 aacgtatgga ttttgctcag atgatcgcgg attttgacgc atttccatgc cgataccgga
    29281 tcaataaata caggcgtttc cccgctattt tccagaaccc agaccagcgc ctcttcacta
    29341 aggttgcaat ccgcgacaat aacctttgca ccgcgaataa actcctgatg ctgggcaaga
    29401 aattctgcag aaatacagtc actaatactc atatcattta tcgcgacaag catttctcct
    29461 gtattatcga gcagtgataa atagctggat gtattctcac ccggaacaat taagcattta
    29521 tcgacataaa caccggattg attagtttgc gccagtaatg attgcccata aaaatcacca
    29581 cctactgcac ttaaaagcca ggcatttttc cccagtaacg ccagattatg agcgatattc
    29641 cttcccacgc cacctggggt aaatttaatt tttccgggat tcgaatccgc ataattcaat
    29701 ggatgatgtg aatacccagc cacatcaata ttcgccgaac caataattac tatgtagtcc
    29761 ttttcgcgca tagcacgccc cctggcaata aattgccaac gaagaaattg ggtaaaatag
    29821 ccattacagt caataagact attagagcac gtgttcagat ttaaacacat gttcataata
    29881 agcatattta acgtaatgta aatcgatcag aaaaaacgaa atcggccaga tatgaaggcg
    29941 gtcacaaagt ttgtctattt atgcagcaac aaaagagaaa gttgtgatgt gtaacacaca
    30001 atatggcagg caagaaaaag ggcagagatg tttctgccct ccagattgtt gacaacgcgc
    30061 gctttgttca tcctggatgt ggcgtaaacg ccttatccga cctacaaaag cgcaaaaaat
    30121 tcaatatatt gcagggttca tgtaggtctg ataagcatag cgcatcaggc aattttgcgt
    30181 ttgtcatcat cggggcagag atgtctcatc cccgataatg aagataaatt atgcgttttt
    30241 ttctactatt tgtgtttccg ggcgtttcag gaaggcatag gccaaacccg ccaccagcgt
    30301 accggcaata attgctacca ggtaacccaa taccggcgta atagcgccag ggatcagcag
    30361 aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca atcgccattg agattgcgcc
    30421 agtcagcgcc ccaccaacga tacagcacgg tagcacacgc atcggatcac gagcagcaaa
    30481 cggaattgca ccttccgaaa taaagcacag accaagtacc agagcggctt tgccaccttc
    30541 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    30601 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    30661 acccacaccg aatgcgtacg ctgctttgtt taccggaccg cccatgtcag tacacatcat
    30721 gccaccgagg atcgccccca gcagaaccgc attcgcagtc cccatggtct gcagccagtg
    30781 agtcaaccca tcgagaatgc cagcaaccgg tttaccgatt aggtagatca tcgccagacc
    30841 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagcgcct ccatactctg
    30901 tggcagtttc aattgcgtac tgattaactt cgcaatgtaa ccagccagga agccagcaat
    30961 tataccgcca ataaagccag aaccggtgct gaccgccagc ataccgccaa tcagacccgg
    31021 tgtgaggccc ggacgatcgg caatggagaa ggcaatataa cctgccagta ccggcaccat
    31081 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    31141 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacaac
    31201 catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcgcctg cactctcttt
    31261 cttaccttca gtggtcgctg tttgagcttt gcccgctggt tcatacggcg ttgcttcagc
    31321 aaccgcttta tccagttcct gcgcagtttt cttcagcgcc agaccggtag aggtgcgata
    31381 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    31441 atccgctgct gcgacttctt caggggtgat tgcattgccc gcgccaacag aaccacgggt
    31501 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    31561 aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt tcggaccgct
    31621 ggcagcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc
    31681 ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa atatcaccca gccagacatt
    31741 tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg ccatttctgc
    31801 gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    31861 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    31921 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    31981 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagctg
    32041 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    32101 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    32161 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacaacat
    32221 gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat cacatctttc atttcaggca
    32281 gtttacggcc tgcccagatt tccaactcgc ggcggttagg tttcaccagc cacggtgccg
    32341 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    32401 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    32461 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    32521 cccagtcggc gagggtgact tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    32581 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    32641 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc aggaagccgc
    32701 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    32761 ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    32821 aaccaacaag gtcataagcc ggattaaggg tgatcgtagc aacacgtctg ctcattatgc
    32881 gccctcccca agaccagcag cgatagcgtc gccaatggct ttcagcgcct gttcagcatc
    32941 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    33001 catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca cggtaatatc
    33061 actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat gcaggccgtg
    33121 ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat catcgctggt
    33181 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattgtcgag
    33241 caataaatca gcgagacgct taagaaccgc gatgggctga tcgtcattca tcgccacact
    33301 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    33361 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gtccgagatt
    33421 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc ccacctcttt
    33481 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga tatccagcgt
    33541 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gtaatgcgcg
    33601 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    33661 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgattgc
    33721 cacgtacgct acctgaccgt caccccaggt gacgccttcc gggaactgaa atacctgaac
    33781 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    33841 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttctgc
    33901 tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt ctttgtctcc
    33961 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    34021 ctgaattgaa acgattcagc ctctatgaga aaaaaagcgc caacctggct tagggttaaa
    34081 gacaagatcg cgctgaaacg tttcaagaaa gcataatact tctgtttcag cacgcaagga
    34141 aagccgtaaa gttatgagca aaagtttgat gtgctgcaca attttttgtc atttttccta
    34201 attgctgatg ggaaaatccg ttatcagcaa tttcatttca gcagcgtcag cagccaatca
    34261 gcaacagtga aatggcattt tgattattcg tggcgctttt gatttcgctg tctgtttatt
    34321 ttctgacaag cagcgtaaac tccgcgtctt cctcttccag tgatcgacct gcatgcataa
    34381 ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt
    34441 tgcctttctc accggtattg cgggtgctct gcaaaccccg acactcagta ttttccttac
    34501 cgatgaagta catgcccgtc cggcgatggt gggattcttc tttaccggca gcgctgtcat
    34561 tgggattctg gtaagtcagt ttctcgctgg gcgctctgat aagcgcggcg atcgcaaatc
    34621 gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc accctttttg cctggaatcg
    34681 caactacttt gttttgctat tcgttggcgt ctttcttagc agctttggct cgaccgctaa
    34741 cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa accggacgtg aggcggtgat
    34801 gttcagctct tttttacgcg ctcaggtttc actggcatgg gtcattggcc caccgctggc
    34861 ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt
    34921 tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg atgcaaaaag agcttccgct
    34981 ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc gatacgctac tgctgtttgt
    35041 catttgtaca ttgatgtggg gctcgaacag cctgtacatc atcaacatgc cgctatttat
    35101 tatcaacgaa ctgcatcttc ccgagaaact ggccgatgtg atgatgggga ccgccgccgg
    35161 gctggaaatc ccgacgatgt tgattgccgg atatttcgcc aaacgtctgg gtaagcgttt
    35221 cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac gcaggaatgc tgatggcgca
    35281 ttcacctgtc attctgttgg gcttgcagct gctaaatgct atttttattg gcattctggg
    35341 cggcatcggg atgctctatt ttcaggatct gatgcccggt caggcgggtt cagccaccac
    35401 gctctatacc aacacttcgc gcgtgggctg gatcatcgca ggatcagtgg cgggcatcgt
    35461 cgccgaaatc tggaattatc acgctgtgtt ctggtttgcg atggtgatga ttatcgccac
    35521 tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg gtcagcattt ccagatcgat
    35581 aagccaggtc atggcctgct gacgcgagtt accgcacatt tcagcgctgg cttgtaagcc
    35641 tgcacagact ttcggtcgca gtggcgaggt gaaaatttta cagcgttgct gttcatcaag
    35701 ctgaatgcag ggcgtattgg cgggcttgcc atctggcata ccgggaatag ggctggaaat
    35761 tgaaggggca gtgcaacacg ccccgcaacc cggacggcat tccatacgtt gttctcttaa
    35821 ggttaaccac cggattgatg agcgcgcaca gtaccacctt ttttgcacca gcaaaagtgc
    35881 gaataccact tgcccgaaag gcccgtcgcg agtactttgt cgcgatattt ttgacatttt
    35941 cgactacagg aatttttcga tgccaagagc gaacgaaatt aaaaaaggta tggtactgaa
    36001 ttacaacggc aaactgctgc tggttaagga tattgatatt cagtcgccca ctgcccgcgg
    36061 tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga aagtagaaga
    36121 gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg acccgccgtt acgttgactt
    36181 ctcctatgtc gatggcaacg aatatgtctt tatggataaa gaagactata ccccgtatac
    36241 cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg gcatgccgga
    36301 catgcaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc agaccgttga
    36361 tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg cccgtaacaa
    36421 accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa gcccgggcga
    36481 aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact aacttcagcc
    36541 gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac caatcttcta
    36601 ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga tcgccctcac
    36661 acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataact ttacactgtc
    36721 attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct ccgttacgct
    36781 cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac gtatcgttca
    36841 ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc gtgtgctgaa
    36901 tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg atcaactgat
    36961 gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg cggacggcaa
    37021 tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg attccttagc
    37081 ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga cgattaccga
    37141 aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc cgcggattat
    37201 tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg tcgaagcact
    37261 gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg ctctcctgcg acaatattcc
    37321 cgacaatggc catgtggtga aaaacgcggt gctgggaatg gcagaaaaat gttcgccaga
    37381 actcgccggg tggataaaag aacacgtcag ttttccggga accatggtcg accgcattgt
    37441 tccggctgca accgacgaat cactggcaga aatcagccag catctggggg taaatgatcc
    37501 ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt gaagataact tcatcgctgg
    37561 gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc catgggaaga
    37621 gatgaaactg cgtatgctta atggcagcca ctcttttctc gcttatctgg gttacctctc
    37681 aggattcgtc catatcagtg attgcatgca ggatcgcgta tttcgccatg ctgccagaac
    37741 attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt taacacaata
    37801 tgcggataag ttaattgcac gttttgctaa tccgtcgctg aaacataaga cctggcaaat
    37861 cgcgatggat ggcagccaga aattaccgca acgtatgctg gcaggtattc gcatacatct
    37921 ggggcgtgaa acggactggt cgttgctggc attaggcgtt gctggctgga tgcgttacgt
    37981 cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta gcgataaaat
    38041 tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc tttccctgcg
    38101 tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca tcgaacaagc
    38161 ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc tcaaaattta
    38221 acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc ttctcttttc
    38281 tgcctttttt agccaggatt aacgctcagt taacttacca gagtattgcg gagccgttgt
    38341 gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt cgattcttca
    38401 tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg aatttgggga
    38461 agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag agatccccgg
    38521 cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata ccttactgcg
    38581 tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc cgaaacagat
    38641 cctcgatctg ttaaccgcac cagtctatga actgtggata gatctgcgcg ccaccttgtg
    38701 cattctcgat ccacgcctgc tgctggacga aaaaagcgcc agcaatgaaa acttccgtga
    38761 ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga cgcccgaaag
    38821 tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat taattcacag
    38881 tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt tagccgagtt
    38941 gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag cagcgttaag
    39001 cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat atcaggcctg
    39061 cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg aatgggcgcg
    39121 acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc tggtacgaat
    39181 caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac cggacagccg
    39241 tattgagctg atttccagca gcgaagctga ctggaatgct ttgcagagcg cgctgttgaa
    39301 gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat aattagagaa
    39361 aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggcct cgcgctgttt
    39421 ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc ggatattttt
    39481 tatttcttta atcagaaact ggtcgaaagt aaggcctttt tgtggctggt tgcattgacc
    39541 aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg gaatgttgat gctgagtttc
    39601 tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct ggtcatgcta
    39661 ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa acgggccagc
    39721 ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa
    39781 gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat tttttcggca
    39841 ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat ttttgtggtt
    39901 tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat tgtcggttcg
    39961 atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag tgatcgatta
    40021 atcacctttt ttgacaaatc actaccggga aaaaacaaac atttccaaaa caaataactc
    40081 acagtaatta acatcatcag gcttattttt atagtgagga taatcctgat gatgcgcacc
    40141 gtgctttcat ccatcgcgcg caaaaatcat tctctaagta aatgaatgga ttgcatgcgt
    40201 ttcactcaat cgtactttaa ttgaccaatc ccgcttatta actttctgta tcactttttc
    40261 ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa tctcgaactc
    40321 gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta gtccacagat
    40381 ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgccg cttggcattt
    40441 ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat ggtcaaatct
    40501 caaccgattt tgagatatat cttgcgcggg atccccgcga ttgcagtagc ggttctgctt
    40561 tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg tgcagtgggt
    40621 agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt tcgtaatgtc
    40681 gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg ttatcgtctg
    40741 ggcggcagca ctaaaaaagg tatcgattgt tctggttttg tacaacgtac attccgtgag
    40801 caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg taaatctgtt
    40861 tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc aacgggacgc
    40921 catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag cagtggtgtt
    40981 attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc acgccgggtt
    41041 ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga cgagttaact
    41101 gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat gatgtttccg
    41161 cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc gtaatttcag
    41221 gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct cctgcttacc
    41281 tgcattgtag caggcgtgat gattgcgata ctggttagct gccttcagtt tttagtggcc
    41341 tggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa gtatctcgat
    41401 acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac cttagatacc
    41461 tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa tgtccgtacg
    41521 tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga gatggacatt
    41581 ccactcaatg aattgattcc ggcgctcgac attaataaaa acgtcgatat ggcgatctta
    41641 cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg caaccctttg
    41701 ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc tctcttttat
    41761 agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac tgcgctatct
    41821 accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt ccgtgaaact
    41881 aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac atggaacgat
    41941 gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct gctctgctat
    42001 tacctgatga gcgtacgtat gcgccccggc agagaaatca tgaccgccat caagcgcgaa
    42061 caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgagt aacgggcctg
    42121 gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa ttcccccgga tgccttcatt
    42181 aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt tgagttaatt
    42241 gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag gcgtcaaatt tggtattaac
    42301 attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa actgctcact
    42361 tccctgcccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa
    42421 gagcaagaag ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt
    42481 gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac gctcgattat
    42541 ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac ttcacccgta
    42601 cttgacgcgg tgctgacgct ggcgaaacgc ctcaatatgc tgacggttgc tgaaggggtc
    42661 gaaacgccgg aacaggcgcg atggctaagc gaacgcggcg ttaatttcat gcaaggctac
    42721 tggattagtc gccctttacc gctggacgat tttgtccgct ggctgaagaa accgtatacg
    42781 ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat gccggattgc
    42841 ggctaatgat gagtaaaagg aaatccattg cagatgattg tgcgcatact gctgctgttt
    42901 atcgctctgt tcacctttgg tgtgcaggcg caggctatca aggaaagcta tgcctttgcc
    42961 gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc
    43021 gcgccaaaag gtgggcagat aacgttgtca gccctcggca ccttcgataa tttcaaccgc
    43081 tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac gctatttacg
    43141 acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagcgc acgctatgct
    43201 gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca tgatggttcg
    43261 cccattactg cccgcgatgt agagtttact tttcaaaaat ttatgaccga aggcgtgccg
    43321 caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcaccgtt aaccgtgcgc
    43381 attgagttag ctaaacccag caaagaagat atgctgagtc tgttttcgct gccgatattt
    43441 ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc tccgcttgcc
    43501 agtggtccgt accgcgttac gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg
    43561 aaagattact gggcagcaaa cttaccggta aaccgtggac gctggaattt cgacaccatt
    43621 cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc aggtgccttt
    43681 gatttgcgta tggaaaacga cgctaaaaac tgggccacgc gttataccgg taaaaatttc
    43741 gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga tacgcgctgg
    43801 ctggcgttta atatccaacg tccggtattc agcgatcgcc gggtccggga agcaatcact
    43861 ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaacgcctg gagtcgcacg
    43921 aacagttatt ttcagaatac cgaatacgcg gccagaaatt accccgacgc cgcggagctg
    43981 gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca aatctaccag
    44041 ccgccggtat ctaaaggcga tggctacgat cgtgacaacc tgttaaaagc cgacaaactt
    44101 ctcaacgaaa cgggctgggt gctgaagggt cagcaacgcg ttaatgccac aacgggtcag
    44161 ccgctcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt attgccgttc
    44221 cagcacagcc tgcaacggct gggtatcaat atggacattc gcaaggtgga taactcgcaa
    44281 atcactaacc gcatgcgcag tcgcgactat gacatgatgc cgcgcctatg gcgggcgatg
    44341 ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa ttccacttat
    44401 aatgcccccg gcgtgcaaag cccggttatc gactcgctga tcaaccaaat tattgccgcg
    44461 cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt attaacgtgg
    44521 aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg gtgggataaa
    44581 ttctcccaac ccgctgtacg ccctgtttac agcctgggta tcgatacctg gtggtatgac
    44641 gttaataaag cggccaaact gccgtcagcc agacaacagg gagagtagat gggcgcttac
    44701 ctgattcgcc gtctgttgct ggtgatccca acgttatggg ctattatcac tatcaacttt
    44761 ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg ccatcgccgc cattgagttt
    44821 ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa
    44881 acgggtgtcg gcaatatcag cgacagtaat taccgtggcg gacgcggatt agacccagaa
    44941 gtgatcgctg agatcactca tcgctacggt ttcgataagc cgatccacga acgttacttc
    45001 aaaatgctct gggactacat ccgctttgat tttggtgata gcctgtttcg cagcgcctcg
    45061 gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt gtggagcacg
    45121 ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta taatgggagt
    45181 cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc ggcctttttg
    45241 tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct gttccctcta
    45301 cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat caccgattat
    45361 ctgtggcata tcacgctacc ggtgctggcg acagtgattg gtggctttgc ggcgctgacc
    45421 atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt
    45481 gcgaaagggg taagtgagaa aaacattctc tggaaacatg tgttccgcaa cgccatgctg
    45541 ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg
    45601 attgaggtga tgttttcact caatggtctt ggcttactgg gctacgaagc gaccgtctcg
    45661 cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg cctgctgctg
    45721 aatattgtca gtgatatcag ctatacgctg gttgatccgc gtatagattt tgagggacgt
    45781 taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgt cataaccgtc
    45841 gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg tgttctgaac
    45901 ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg cagttggtat ttcccgttgt
    45961 tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct gattatcagg
    46021 acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca ccgattcgct
    46081 ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca ccctcccggc
    46141 aaaactggct gggaacggat gccaacggcg gcgatgtgct ggcacgcatt ctctatggca
    46201 cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc
    46261 tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctgggga caacgcttta
    46321 ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc agcgtcgtac
    46381 agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg agtctggtcg
    46441 gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga ctacattcgt gcggcacagg
    46501 cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat gccatggtcg
    46561 cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg acctcgctcg
    46621 atttcctcgg cttcggtcta ccgctcggtt caccgtcact cggtgaactg ctgttacaag
    46681 ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat
    46741 tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat cctaataagg
    46801 cggtgtagca tgacgcaaac tctgttagcg attgaaaatt tgtcggtggg ttttcgccat
    46861 cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc tggcgaaacg
    46921 ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc
    46981 ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttca tggcgaatcg
    47041 ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt
    47101 tttcaggaac cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca gctttatgaa
    47161 gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gtggcgaaat tcttaactgc
    47221 cttgatcgcg tcggtatccg ccaggcggca aaacggctga cagattatcc gcatcagctc
    47281 tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg accggaatta
    47341 ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca gattttacag
    47401 ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac tcataacctc
    47461 agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag
    47521 caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca aaagctactc
    47581 aacagtgaac cgtcaggcga tccggtaccg ttgccagaac ctgcctcaac gttgctggat
    47641 gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg cattgtggat
    47701 cataatgtgg tggtgaaaaa catcagtttt acgctacggg cgggtgaaac actgggttta
    47761 gtgggcgagt ctggttccgg gaaaagcacg acgggactgg cgctgctgcg actgattacc
    47821 tctcagggca gcatcgtctt tgacggtcag ccactgcaag atttaaatcg ccgccagctg
    47881 ttacctactc gtcatcgtat tcaggtggta tttcaggatc caaactcctc gctcaaccca
    47941 cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc gacgctttct
    48001 gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt agatcctgaa
    48061 acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat tgcgattgcc
    48121 agggcattaa ttcttaagcc ctcactgatc atacttgatg aaccgacatc atcactcgac
    48181 aaaacggtac aggcgcaaat attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg
    48241 gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca tcaggttatc
    48301 gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt tgccgcaccg
    48361 cagcaggagt atacgcgtca gctactggcg ttgagctgac gcttaaaaag gattgtagtt
    48421 tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg cagcaaactc
    48481 gtcctggcga tttacaaaca ggcacggctc accttcacac tccagcaccg aacatggaac
    48541 ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat tatcaccatc
    48601 cgggctaagc aatttaagcg ccaccaggct gttgtcaccg cacggtccat tttgcggaat
    48661 cggttcaacc ttcgaacctg cgaaaccagc agaccagcga aagttctgcg gtagacgatg
    48721 gacgattgag agttgtaatg aagtcactta ttttccccgg aagcacattt acttcacaat
    48781 ttatttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    48841 gatgcctcgc ctgcatggcc tctatggctc tgatttaagt aatatctgcg ggcgtggtca
    48901 cgtttgcggg gctatatgta cccgtttctg
//
LOCUS       DAZPLM010000005        48808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPLM010000005 DAZPLM010000000
VERSION     DAZPLM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.005"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHVC7_000234"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHVC7_000234"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR2114390.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHVC7_000235"
     CDS             complement(1496..3121)
                     /locus_tag="ACHVC7_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR2114391.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="ACHVC7_000236"
     tRNA            complement(3580..3666)
                     /locus_tag="ACHVC7_000236"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHVC7_000237"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHVC7_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR2114392.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHVC7_000238"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHVC7_000238"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR2114393.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHVC7_000239"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHVC7_000239"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR2114394.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHVC7_000240"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHVC7_000240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR2114395.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHVC7_000241"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHVC7_000241"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR2114396.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHVC7_000242"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHVC7_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR2114397.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHVC7_000243"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHVC7_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR2114398.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="ACHVC7_000244"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="ACHVC7_000244"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR2114399.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11959..12066
                     /locus_tag="ACHVC7_000245"
                     /pseudo
     CDS             11959..12066
                     /locus_tag="ACHVC7_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHVC7_000246"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHVC7_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR2114400.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="ACHVC7_000247"
     CDS             complement(13278..14243)
                     /locus_tag="ACHVC7_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2114401.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHVC7_000248"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHVC7_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR2114402.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHVC7_000249"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHVC7_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR2114403.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="ACHVC7_000250"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="ACHVC7_000250"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR2114404.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHVC7_000251"
     CDS             16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHVC7_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR2114405.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHVC7_000252"
     CDS             complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHVC7_000252"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR2114406.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHVC7_000253"
     CDS             complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHVC7_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR2114407.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHVC7_000254"
     CDS             complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHVC7_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR2114408.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHVC7_000255"
     CDS             complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHVC7_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR2114409.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHVC7_000256"
     CDS             complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHVC7_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR2114410.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHVC7_000257"
     CDS             complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHVC7_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR2114411.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHVC7_000258"
     CDS             complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHVC7_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR2114412.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21783..22760
                     /locus_tag="ACHVC7_000259"
     CDS             21783..22760
                     /locus_tag="ACHVC7_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR2114413.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHVC7_000260"
     CDS             22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHVC7_000260"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR2114414.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHVC7_000261"
     CDS             23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHVC7_000261"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR2114415.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24344..24919
                     /gene="lptC"
                     /locus_tag="ACHVC7_000262"
     CDS             24344..24919
                     /gene="lptC"
                     /locus_tag="ACHVC7_000262"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR2114416.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24888..25445
                     /gene="lptA"
                     /locus_tag="ACHVC7_000263"
     CDS             24888..25445
                     /gene="lptA"
                     /locus_tag="ACHVC7_000263"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR2114417.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25452..26177
                     /gene="lptB"
                     /locus_tag="ACHVC7_000264"
     CDS             25452..26177
                     /gene="lptB"
                     /locus_tag="ACHVC7_000264"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR2114418.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHVC7_000265"
     CDS             26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHVC7_000265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR2114419.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27681..27968
                     /gene="hpf"
                     /locus_tag="ACHVC7_000266"
     CDS             27681..27968
                     /gene="hpf"
                     /locus_tag="ACHVC7_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR2114420.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHVC7_000267"
     CDS             28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHVC7_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR2114421.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHVC7_000268"
     CDS             28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHVC7_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR2114422.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29474..29746
                     /gene="npr"
                     /locus_tag="ACHVC7_000269"
     CDS             29474..29746
                     /gene="npr"
                     /locus_tag="ACHVC7_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR2114423.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHVC7_000270"
     CDS             29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHVC7_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR2114424.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHVC7_000271"
     CDS             complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHVC7_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR2114425.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHVC7_000272"
     CDS             complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHVC7_000272"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR2114426.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHVC7_000273"
     CDS             complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHVC7_000273"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR2114427.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34629..35558)
                     /locus_tag="ACHVC7_000274"
     CDS             complement(34629..35558)
                     /locus_tag="ACHVC7_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR2114428.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36233..40693
                     /gene="gltB"
                     /locus_tag="ACHVC7_000275"
     CDS             36233..40693
                     /gene="gltB"
                     /locus_tag="ACHVC7_000275"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR2114429.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFCQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40706..42124
                     /gene="gltD"
                     /locus_tag="ACHVC7_000276"
     CDS             40706..42124
                     /gene="gltD"
                     /locus_tag="ACHVC7_000276"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR2114430.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42308..42712
                     /locus_tag="ACHVC7_000277"
                     /pseudo
     CDS             42308..42712
                     /locus_tag="ACHVC7_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42804..43109
                     /locus_tag="ACHVC7_000278"
     CDS             42804..43109
                     /locus_tag="ACHVC7_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2114431.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHVC7_000279"
     CDS             complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHVC7_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR2114432.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHVC7_000280"
     CDS             complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHVC7_000280"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR2114433.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44518..45207)
                     /locus_tag="ACHVC7_000281"
     CDS             complement(44518..45207)
                     /locus_tag="ACHVC7_000281"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR2114434.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHVC7_000282"
     CDS             complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHVC7_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR2114435.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHVC7_000283"
     CDS             complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHVC7_000283"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR2114436.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHVC7_000284"
     CDS             complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHVC7_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR2114437.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        12154 a        13128 c        12065 g        11461 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac
    16321 aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt
    16381 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16441 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16501 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    16561 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16621 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16681 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16741 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    16801 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16861 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16921 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    16981 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17041 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17101 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17161 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17221 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    17281 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    17341 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17401 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17461 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17521 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    17581 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17641 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17701 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17761 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17821 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    17881 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    17941 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18001 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18061 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18121 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18181 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18241 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18301 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    18361 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18421 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18481 agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc gtttaccccc
    18541 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18601 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18661 gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac
    18721 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18781 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    18841 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18901 tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg aagtccagac
    18961 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    19021 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19081 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    19141 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19201 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19261 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    19321 gattggtctg gtctgcctcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    19381 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19441 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19501 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    19561 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19621 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19681 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19741 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    19801 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    19861 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    19921 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    19981 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20041 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20101 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    20161 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    20221 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    20281 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    20341 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20401 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20461 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20521 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20581 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    20641 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20701 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20761 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20821 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20881 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    20941 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21001 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    21061 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    21121 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    21181 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggtagtt gggtatgttc
    21241 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21301 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21361 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21421 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    21481 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21541 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21601 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    21661 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21721 ccgttacggc tttctgaaaa tcttcagcgg accgacgagt atacctgaag aaaggacgtt
    21781 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21841 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21901 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    21961 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    22021 atattttgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg
    22081 ttctgcgtcg tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct
    22141 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22201 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22261 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    22321 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    22381 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22441 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22501 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    22561 cgctgataac gccaggagag attgagccat tggcgtacag tcgtgactac agcgtgatgt
    22621 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    22681 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22741 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22801 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22861 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22921 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca
    22981 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23041 gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23101 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23161 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23221 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    23281 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23341 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23401 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    23461 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23521 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23581 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    23641 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23701 ggcgaccatt tactcggagt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23761 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23821 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    23881 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    23941 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24001 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24061 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24121 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24181 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24241 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    24301 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24361 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcattg atatggccga
    24421 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24481 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24541 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24601 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24661 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    24721 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24781 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24841 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24901 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    24961 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    25021 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    25081 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25141 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25201 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25261 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25321 taaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25381 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    25441 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25501 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25561 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    25621 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25681 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    25741 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25801 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25861 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25921 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    25981 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    26041 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26101 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26161 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26221 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26281 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26341 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26401 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26461 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26521 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    26581 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    26641 tattgctacc tctatcgtcg atgccgttga tgacatcggt tatctgactg tcccgctgga
    26701 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    26761 taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct
    26821 gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat
    26881 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    26941 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27001 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27061 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27121 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    27181 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27241 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    27301 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    27361 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27421 gaagtatttc ttttccagtc acatcaatac cgagggcggc ggcgaagctt cctccacggc
    27481 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27541 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27601 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27661 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27721 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27781 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27841 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27901 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    27961 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28021 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28081 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28141 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28201 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    28261 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28321 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28381 tcgacaacca accggtggac ttactttttg ccctgctggt gtcggcagac caaactaaaa
    28441 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    28501 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28561 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28621 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28681 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28741 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    28801 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28861 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28921 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    28981 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29041 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    29101 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29161 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29221 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29281 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29341 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29401 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    29461 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29521 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29581 acgtaatgac gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga
    29641 ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact
    29701 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29761 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    29821 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    29881 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    29941 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30001 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30061 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30121 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30181 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaactct ccattacgct
    30241 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    30301 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30361 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    30421 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30481 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30541 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    30601 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30661 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    30721 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30781 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30841 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    30901 cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga
    30961 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    31021 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31081 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31141 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    31201 aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    31261 taacagcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31321 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31381 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31441 tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31501 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    31561 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31621 tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg
    31681 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31741 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc
    31801 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31861 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31921 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    31981 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    32041 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32101 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32161 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32221 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32281 accgacgtta tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg
    32341 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    32401 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32461 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32521 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32581 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32641 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32701 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt
    32761 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gtaacaccag
    32821 gtcgtattcg cccggtttaa acatctctag cgccgccttg ccggtcatgg cgacatcaac
    32881 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    32941 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    33001 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33061 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33121 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33181 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33241 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    33301 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    33361 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    33421 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33481 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    33541 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    33601 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    33661 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    33721 ccgcttacgc tcggtaatgt ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33781 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    33841 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33901 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    33961 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34021 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    34081 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34141 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34201 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34261 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34321 gtagaccgcc cacggcgtaa tcaacaaacc aaagaagata gaacgaataa catcaatgct
    34381 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34441 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34501 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34561 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34621 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34681 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga
    34741 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34801 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    34861 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg caacggatgt
    34921 agctttatac cgtcaacacc ggtttccact acccgctcca gcgtttgcag acattcagcc
    34981 agcccttcgc ctggcagacc gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt
    35041 gccagctgcg tcgtgcgctg ataacaggcg aaatcatgac cgcgattaat gcgatgtagc
    35101 gttttgtcgt gcacggtttg cagccccagc tccagccaca cttcgtagcc ctggtcttta
    35161 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac
    35221 aaaccgacaa tattggcctg gctcaccgcc tgttgataca tcgaacgcag cacctgaact
    35281 tcggcaaagg tgctggtata cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt
    35341 aaattcgcct gatgcgccag ctgttcggca atggaacgat gctgctgcgc ttcgtcggca
    35401 aacgaggcaa cattacagaa ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg
    35461 caactgaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35521 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35581 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    35641 ggcccgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    35701 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35761 aacagtagca tgaaacgtca ttaccaatta atgcagtata aaatgctggt tttctcgtca
    35821 attcaaggca ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct
    35881 aacgacttcc cttttagcct taaagataaa atctatttta atttcagcca tttaataaag
    35941 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    36001 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    36061 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    36121 gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt
    36181 gcgcagcggt tcggtagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    36241 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    36301 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    36361 cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga
    36421 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt
    36481 cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    36541 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    36601 aggcgtactg ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa tttttgtgaa
    36661 cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    36721 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctat cgaatctggt
    36781 gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc
    36841 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    36901 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    36961 catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat
    37021 ccctgatctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    37081 ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    37141 gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    37201 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    37261 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    37321 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    37381 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    37441 tgggcatatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    37501 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    37561 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    37621 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    37681 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    37741 cgactacttc tgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    37801 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    37861 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    37921 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    37981 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    38041 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    38101 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    38161 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    38221 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    38281 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    38341 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    38401 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    38461 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    38521 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    38581 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    38641 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    38701 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    38761 caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgctgcgat
    38821 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    38881 cggcggtaac tcaaactccg gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa
    38941 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    39001 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca
    39061 gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt
    39121 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    39181 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    39241 accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat
    39301 cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg
    39361 cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca
    39421 taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc
    39481 gattctcggc gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa
    39541 atatctgcgt atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa
    39601 actgcgtaag aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat
    39661 cgcccgtgaa acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat
    39721 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc
    39781 gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga
    39841 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    39901 gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc
    39961 cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    40021 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc
    40081 gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc
    40141 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    40201 tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    40261 cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg
    40321 cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt
    40381 taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt
    40441 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca
    40501 tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg
    40561 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    40621 gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt
    40681 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    40741 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    40801 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    40861 cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc
    40921 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    40981 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    41041 tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga
    41101 aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat
    41161 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    41221 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    41281 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    41341 attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta
    41401 cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    41461 agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat
    41521 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    41581 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    41641 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    41701 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga
    41761 agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc
    41821 ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    41881 agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    41941 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    42001 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    42061 ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    42121 ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct
    42181 ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt
    42241 taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg
    42301 acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg
    42361 tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat
    42421 gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc
    42481 aagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta
    42541 taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca
    42601 acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac
    42661 accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg
    42721 ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg
    42781 caaataaacg aaggaaagtt gccgtgattt acattgaaca gcttgaatta atccataaaa
    42841 gtggtgatgt gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc
    42901 accttgttcc attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag
    42961 aagcaattcg gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa
    43021 ctattacgaa taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg
    43081 ttaacataaa agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg
    43141 gctctgtgcc aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca
    43201 gccttaacct tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc
    43261 ttatgcggct ctcctagcat aaacacggcg aacattcccg gtttcaggat gatggcttgc
    43321 tcgttttcaa tggtgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    43381 cgcgcagtgc ctgccatgcc aaacagaatc cgttcttcac cgtttaataa cagctggata
    43441 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    43501 atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    43561 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    43621 ggtaatgacc gtacttcacc catcatcata atttttctcc ctgggccaac agcgctgccc
    43681 caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga aataccgctg
    43741 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    43801 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    43861 gcacatgtgc ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg
    43921 tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc
    43981 gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg
    44041 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttac
    44101 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    44161 catcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa
    44221 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    44281 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    44341 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    44401 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    44461 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    44521 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    44581 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    44641 cgtgttgtaa cgcccttcgg caatcacccg acatccggct tcgctcaacg ttttcaccag
    44701 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    44761 aatttcggcg cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    44821 cgccagtaaa ccgtgatgat gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    44881 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc
    44941 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    45001 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt cgatgcgaat
    45061 ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt cgggtttatc
    45121 gagcgggctg tcaggaatcg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    45181 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    45241 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    45301 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    45361 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    45421 aggctgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    45481 ccgatgattg gggccagtgc gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc
    45541 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    45601 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    45661 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcaggctaca aacgtacgct
    45721 ttgcgggttc ccagccagtc accgaggaag cctcctacgc agcatcccac ggccgcgcca
    45781 aagccactaa agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc
    45841 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    45901 accatcagca ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata
    45961 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    46021 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    46081 tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc
    46141 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    46201 aaaatgccga taaagaacag cgcacgccaa ccccagaccg gaaccaccag gctatagacc
    46261 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    46321 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    46381 atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg cgcaaagccg
    46441 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    46501 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    46561 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    46621 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    46681 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    46741 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    46801 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    46861 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    46921 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    46981 tttcaggccg cggaatacgc cagttttgat cagtaaatca atgactttgt tgcactcagt
    47041 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    47101 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    47161 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    47221 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    47281 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    47341 cacaaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat cgcagtgttc
    47401 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    47461 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    47521 gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt cacgctcgga
    47581 aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt cgatgccctg
    47641 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttggtc
    47701 aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa atacctctga
    47761 agtgatactt gtctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    47821 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    47881 tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    47941 acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc gcaatatgct
    48001 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    48061 cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta ccagggatct
    48121 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    48181 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt
    48241 cagccgcata gcgcaccaga ctggattcaa agaacagacg taattgttcg aaatgggcaa
    48301 tcccaccggg atgagaaagg aaatctttcg ccatgccgga aaactcaccg atgatagtgt
    48361 ccgcagaagg acgcgagacg cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc
    48421 gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag aacgccatca
    48481 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    48541 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt
    48601 tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    48661 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    48721 gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac ggaataatta
    48781 actattgcga aaaattaatg taacgcag
//
LOCUS       DAZPLM010000006        48545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPLM010000006 DAZPLM010000000
VERSION     DAZPLM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.006"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            362..1579
                     /gene="alaA"
                     /locus_tag="ACHVC7_000285"
     CDS             362..1579
                     /gene="alaA"
                     /locus_tag="ACHVC7_000285"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR2114438.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1663..2262
                     /gene="yfbR"
                     /locus_tag="ACHVC7_000286"
     CDS             1663..2262
                     /gene="yfbR"
                     /locus_tag="ACHVC7_000286"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR2114439.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2321..4153)
                     /locus_tag="ACHVC7_000287"
     CDS             complement(2321..4153)
                     /locus_tag="ACHVC7_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR2114440.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4240..4888)
                     /gene="hxpA"
                     /locus_tag="ACHVC7_000288"
                     /pseudo
     CDS             complement(4240..4888)
                     /gene="hxpA"
                     /locus_tag="ACHVC7_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4899..5393)
                     /locus_tag="ACHVC7_000289"
     CDS             complement(4899..5393)
                     /locus_tag="ACHVC7_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR2114441.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5476..5931)
                     /gene="yfbV"
                     /locus_tag="ACHVC7_000290"
     CDS             complement(5476..5931)
                     /gene="yfbV"
                     /locus_tag="ACHVC7_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR2114442.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6269..7471
                     /gene="ackA"
                     /locus_tag="ACHVC7_000291"
     CDS             6269..7471
                     /gene="ackA"
                     /locus_tag="ACHVC7_000291"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR2114443.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7546..9690
                     /gene="pta"
                     /locus_tag="ACHVC7_000292"
     CDS             7546..9690
                     /gene="pta"
                     /locus_tag="ACHVC7_000292"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR2114444.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9880..11400
                     /gene="yfcC"
                     /locus_tag="ACHVC7_000293"
     CDS             9880..11400
                     /gene="yfcC"
                     /locus_tag="ACHVC7_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR2114445.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11433..11975)
                     /gene="yfcD"
                     /locus_tag="ACHVC7_000294"
     CDS             complement(11433..11975)
                     /gene="yfcD"
                     /locus_tag="ACHVC7_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR2114446.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12033..12584)
                     /gene="yfcE"
                     /locus_tag="ACHVC7_000295"
     CDS             complement(12033..12584)
                     /gene="yfcE"
                     /locus_tag="ACHVC7_000295"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR2114447.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12640..13284)
                     /gene="yfcF"
                     /locus_tag="ACHVC7_000296"
     CDS             complement(12640..13284)
                     /gene="yfcF"
                     /locus_tag="ACHVC7_000296"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR2114448.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13420..14067
                     /gene="yfcG"
                     /locus_tag="ACHVC7_000297"
     CDS             13420..14067
                     /gene="yfcG"
                     /locus_tag="ACHVC7_000297"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR2114449.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14124..14486
                     /gene="folX"
                     /locus_tag="ACHVC7_000298"
     CDS             14124..14486
                     /gene="folX"
                     /locus_tag="ACHVC7_000298"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR2114450.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14507..15400
                     /locus_tag="ACHVC7_000299"
     CDS             14507..15400
                     /locus_tag="ACHVC7_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR2114451.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15448..16338)
                     /gene="rpnB"
                     /locus_tag="ACHVC7_000300"
     CDS             complement(15448..16338)
                     /gene="rpnB"
                     /locus_tag="ACHVC7_000300"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR2114452.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16535..17308)
                     /gene="hisP"
                     /locus_tag="ACHVC7_000301"
     CDS             complement(16535..17308)
                     /gene="hisP"
                     /locus_tag="ACHVC7_000301"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR2114453.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17316..18032)
                     /locus_tag="ACHVC7_000302"
     CDS             complement(17316..18032)
                     /locus_tag="ACHVC7_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2114454.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18029..18715)
                     /gene="hisQ"
                     /locus_tag="ACHVC7_000303"
     CDS             complement(18029..18715)
                     /gene="hisQ"
                     /locus_tag="ACHVC7_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR2114455.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18805..19587)
                     /gene="hisJ"
                     /locus_tag="ACHVC7_000304"
     CDS             complement(18805..19587)
                     /gene="hisJ"
                     /locus_tag="ACHVC7_000304"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR2114456.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19808..20590)
                     /gene="argT"
                     /locus_tag="ACHVC7_000305"
     CDS             complement(19808..20590)
                     /gene="argT"
                     /locus_tag="ACHVC7_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR2114457.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20856..21425)
                     /gene="ubiX"
                     /locus_tag="ACHVC7_000306"
     CDS             complement(20856..21425)
                     /gene="ubiX"
                     /locus_tag="ACHVC7_000306"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR2114458.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21520..23037)
                     /gene="purF"
                     /locus_tag="ACHVC7_000307"
     CDS             complement(21520..23037)
                     /gene="purF"
                     /locus_tag="ACHVC7_000307"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR2114459.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23074..23562)
                     /gene="cvpA"
                     /locus_tag="ACHVC7_000308"
     CDS             complement(23074..23562)
                     /gene="cvpA"
                     /locus_tag="ACHVC7_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR2114460.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23821..24483)
                     /gene="dedD"
                     /locus_tag="ACHVC7_000309"
     CDS             complement(23821..24483)
                     /gene="dedD"
                     /locus_tag="ACHVC7_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR2114461.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24473..25741)
                     /gene="folC"
                     /locus_tag="ACHVC7_000310"
     CDS             complement(24473..25741)
                     /gene="folC"
                     /locus_tag="ACHVC7_000310"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR2114462.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25811..26725)
                     /gene="accD"
                     /locus_tag="ACHVC7_000311"
     CDS             complement(25811..26725)
                     /gene="accD"
                     /locus_tag="ACHVC7_000311"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR2114463.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26881..27540)
                     /locus_tag="ACHVC7_000312"
     CDS             complement(26881..27540)
                     /locus_tag="ACHVC7_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2114464.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27623..28435)
                     /gene="truA"
                     /locus_tag="ACHVC7_000313"
     CDS             complement(27623..28435)
                     /gene="truA"
                     /locus_tag="ACHVC7_000313"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR2114465.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28435..29448)
                     /locus_tag="ACHVC7_000314"
     CDS             complement(28435..29448)
                     /locus_tag="ACHVC7_000314"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR2114466.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29514..30650)
                     /gene="pdxB"
                     /locus_tag="ACHVC7_000315"
     CDS             complement(29514..30650)
                     /gene="pdxB"
                     /locus_tag="ACHVC7_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR2114467.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30749..31744
                     /gene="flk"
                     /locus_tag="ACHVC7_000316"
     CDS             30749..31744
                     /gene="flk"
                     /locus_tag="ACHVC7_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR2114468.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31741..32919)
                     /locus_tag="ACHVC7_000317"
     CDS             complement(31741..32919)
                     /locus_tag="ACHVC7_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2114469.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33194..34414)
                     /gene="fabB"
                     /locus_tag="ACHVC7_000318"
     CDS             complement(33194..34414)
                     /gene="fabB"
                     /locus_tag="ACHVC7_000318"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR2114470.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34573..36579
                     /gene="mnmC"
                     /locus_tag="ACHVC7_000319"
     CDS             34573..36579
                     /gene="mnmC"
                     /locus_tag="ACHVC7_000319"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR2114471.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36700..36978)
                     /locus_tag="ACHVC7_000320"
     CDS             complement(36700..36978)
                     /locus_tag="ACHVC7_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR2114472.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37012..37560)
                     /gene="epmC"
                     /locus_tag="ACHVC7_000321"
     CDS             complement(37012..37560)
                     /gene="epmC"
                     /locus_tag="ACHVC7_000321"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR2114473.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37560..38369)
                     /locus_tag="ACHVC7_000322"
     CDS             complement(37560..38369)
                     /locus_tag="ACHVC7_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR2114474.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38369..39193)
                     /gene="mepA"
                     /locus_tag="ACHVC7_000323"
     CDS             complement(38369..39193)
                     /gene="mepA"
                     /locus_tag="ACHVC7_000323"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR2114475.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39197..40282)
                     /gene="aroC"
                     /locus_tag="ACHVC7_000324"
     CDS             complement(39197..40282)
                     /gene="aroC"
                     /locus_tag="ACHVC7_000324"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR2114476.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40317..41249)
                     /gene="prmB"
                     /locus_tag="ACHVC7_000325"
     CDS             complement(40317..41249)
                     /gene="prmB"
                     /locus_tag="ACHVC7_000325"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR2114477.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41415..41966
                     /gene="smrB"
                     /locus_tag="ACHVC7_000326"
     CDS             41415..41966
                     /gene="smrB"
                     /locus_tag="ACHVC7_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR2114478.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42088..42960)
                     /locus_tag="ACHVC7_000327"
     CDS             complement(42088..42960)
                     /locus_tag="ACHVC7_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR2114479.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42947..43471)
                     /locus_tag="ACHVC7_000328"
     CDS             complement(42947..43471)
                     /locus_tag="ACHVC7_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114480.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43468..43938)
                     /locus_tag="ACHVC7_000329"
     CDS             complement(43468..43938)
                     /locus_tag="ACHVC7_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114481.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43935..44483)
                     /locus_tag="ACHVC7_000330"
     CDS             complement(43935..44483)
                     /locus_tag="ACHVC7_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114482.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44458..45209)
                     /locus_tag="ACHVC7_000331"
                     /pseudo
     CDS             complement(44458..45209)
                     /locus_tag="ACHVC7_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45229..47871)
                     /gene="yfcU"
                     /locus_tag="ACHVC7_000332"
                     /pseudo
     CDS             complement(45229..47871)
                     /gene="yfcU"
                     /locus_tag="ACHVC7_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47953..48516)
                     /locus_tag="ACHVC7_000333"
     CDS             complement(47953..48516)
                     /locus_tag="ACHVC7_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114483.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12053 a        13058 c        11967 g        11467 t
ORIGIN      
        1 ccttgccccc attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
       61 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
      121 tacagataaa aaaaccagac ttgcaattaa gaatcagaac aattcactat ataacattgc
      181 atgcaaagca tatacacctc attattttgt cattattaag ttattaacag cacaatcgag
      241 ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac
      301 attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt
      361 catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc
      421 ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat
      481 cggcaaccca gccccattcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg
      541 caacctgcct accgctcaag ggtattgcga ttccaaaggg ctttactccg cgcgtaaagc
      601 catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat
      661 cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga
      721 cgaaatgttg gttcctgcac cagattaccc actctggacc gcggcggttt cgctttccag
      781 cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga
      841 tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc caaataaccc
      901 aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca
      961 taatctcatt atcttcgccg atgaaattta tgacaaaatt ctctacgacg atgctgagca
     1021 tcactcaatt gcgccactgg cacctgacct gctgaccatt acctttaacg gactgtcgaa
     1081 aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca
     1141 cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt
     1201 tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat
     1261 tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat
     1321 tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc
     1381 caaacgcttt aacattcacg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa
     1441 agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt
     1501 cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc
     1561 tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagcgttatc
     1621 ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt
     1681 tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgcgg
     1741 acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct
     1801 atcaaaaatc gaaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg
     1861 taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat
     1921 tcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa actggtcgat
     1981 atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc
     2041 gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg
     2101 gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg
     2161 cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc
     2221 catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc
     2281 gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg
     2341 ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa
     2401 agtcactgaa gctgtaattt cccggaccta aaaccagtgt gttaacaggt gaagaaaccg
     2461 gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcgaatgga taaggcgaca
     2521 cccccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat
     2581 tagagataaa tagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg
     2641 gcccgtaacc accgccaatg tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt
     2701 tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa
     2761 tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga
     2821 tgatagcggc aacaggatta ggaatttcat ctgtcagcat taacgccacc attagcacca
     2881 gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg
     2941 gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt
     3001 tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca
     3061 gcgccacgcc attgcgcttt agccccacca cattcagtcc gtagcgggta cgaaaaccaa
     3121 tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc
     3181 ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt
     3241 gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt
     3301 cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc
     3361 agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc
     3421 gttgaccaat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc
     3481 gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct
     3541 gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca
     3601 cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca
     3661 attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgcttatca
     3721 gcccggcaaa actcaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa
     3781 cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata aacgcgccaa
     3841 gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgcaactt
     3901 tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa
     3961 taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga
     4021 ctgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac
     4081 gcactctgcc cgtcgcaaac aagacaatgg caacggccag taatgaaaga acccaaatca
     4141 attcaccgtt cacaacttat ccttgttaat tgagggagat gtcttgattc tgccataaaa
     4201 aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc
     4261 atttggctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt
     4321 caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tgacaacccg ccgccaggcc
     4381 agaaagcacg ccagcggagc atcttccacc accacacact cctgcggcgc aagccccagc
     4441 agctgcgcgc ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
     4501 gttacaaaca cctctggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc
     4561 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg actgagtaag
     4621 gcgattgccc ccggaagcgg gtaatacctt cggtttccgt ggcctcgatg tgctccagac
     4681 gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcagagagg
     4741 tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc
     4801 tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa
     4861 gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcattgat aatttgatta
     4921 atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc
     4981 tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg
     5041 gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg
     5101 gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa
     5161 ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag
     5221 gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa tccgtaacct
     5281 cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc
     5341 atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac
     5401 tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc
     5461 gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg
     5521 cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg
     5581 tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg cagggggtaa
     5641 tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa
     5701 cagtgcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc agcagagagt
     5761 aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt
     5821 ttcgacaaag accggggcaa ggcgtttttc cagtggccac gtctttgagt aatgctgtcc
     5881 gcggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct
     5941 caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt
     6001 caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaag
     6061 tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg
     6121 caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga
     6181 tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt
     6241 tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg
     6301 cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc
     6361 tggtttagcc gaatgtttcc acctgcctga agcacgtatc aaatggaaaa tggacggcaa
     6421 taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt
     6481 taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg
     6541 tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca
     6601 gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat
     6661 cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac
     6721 cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgcctt acaacctgta
     6781 caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca
     6841 ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct
     6901 gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg
     6961 cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat
     7021 catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac
     7081 caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga
     7141 caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc
     7201 gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac
     7261 tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt
     7321 gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt
     7381 catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat
     7441 cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc
     7501 tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt
     7561 atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt
     7621 gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc
     7681 ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg
     7741 gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat
     7801 gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt
     7861 ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa
     7921 atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg
     7981 gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc caaaaacacc
     8041 aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact
     8101 cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat
     8161 ccggcgaagc tgcaagaatc cagccctctg ccggttctcg gcgctgtgcc gtggagcttt
     8221 gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac
     8281 gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg
     8341 cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac
     8401 gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc cctgctgctg
     8461 actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc
     8521 ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag cctgcagagc
     8581 ttcaacctgg aagttccggt tgacgatcac gaacgtatcg agaaagttca ggaatacgtt
     8641 gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga gcgcagccgt
     8701 cgtctgtctc cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa
     8761 cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct
     8821 gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca
     8881 gcgtctcagg gtgtagaact gggtgcaggg attgaaatcg ttgatccaga agtggttcgc
     8941 gaaagctatg ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac cgaaaccgtt
     9001 gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa
     9061 gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg
     9121 cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg
     9181 ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac cgctgaacag
     9241 ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc
     9301 gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt
     9361 cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg
     9421 cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt
     9481 gcaggtcgcg ctaccgtgtt tatcttcccg gatctgaaca ccggtaacac cacctacaaa
     9541 gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaag
     9601 ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg
     9661 actgcgattc agtctgcaca gcagcagtaa cctcgtcatc atccgcagct ttgcgctgcg
     9721 gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat ctgcattaat
     9781 cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatt tcactttaca
     9841 tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc
     9901 aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt
     9961 ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag
    10021 tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt
    10081 ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat
    10141 gaacgcccgg gcctgatgaa cttcccgttt gaaggattaa cctcaggatc gaaatacggg
    10201 acagctgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg
    10261 cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa
    10321 attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg
    10381 ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat
    10441 gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt tgccagttcg
    10501 tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc ggtgctttct
    10561 ggctccgggt tgcgcatcgt ggtgtgggtt atcgccacta tgattggcct gatctttacc
    10621 atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac
    10681 cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg
    10741 ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt gatcgttaat
    10801 gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc
    10861 atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc
    10921 gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg
    10981 ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt taaataccat cctcaacagc
    11041 attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc
    11101 caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt aaccatgccg
    11161 ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcattc
    11221 cagtttggtg atggcttcag tcacatcatt tacccaacct cagcttcgtt aatggcaact
    11281 ctcggtgtgt gcagggtgga cttccgtaac tggctgaagg tgggcgcgac cctacttgga
    11341 ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg ctaccactaa
    11401 aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct
    11461 tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac
    11521 tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct
    11581 tcctgtaggg cgaagggacc gtgagagacg cagctgaaca acgcgcccca gacacggcaa
    11641 tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac
    11701 tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg aaccacaccg
    11761 cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc
    11821 agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt
    11881 gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc
    11941 acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc
    12001 gctcttacgg cgcattttga attgtgggta aattacggat taatcgcgac ctgcgcaatg
    12061 atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc
    12121 gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc
    12181 cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt ctggtttaaa
    12241 gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc
    12301 agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg
    12361 ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg
    12421 actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg
    12481 ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc
    12541 gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg
    12601 aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga
    12661 aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag
    12721 gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt
    12781 atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca aatgttctgc
    12841 catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc
    12901 ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag
    12961 ccaggcctga atccgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc
    13021 ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa
    13081 ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt
    13141 cggcttcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt
    13201 ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg catctgacca
    13261 aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac
    13321 tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta
    13381 tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc
    13441 ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc
    13501 ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg
    13561 cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgcta
    13621 agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg actctttttg
    13681 agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca ggtaggcgga
    13741 ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct
    13801 tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact aaacaagcgg
    13861 ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg
    13921 ccgtgggtta atgcctggac tcgccagcga attgacctcg caatgtatcc ggcagtcaag
    13981 aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa
    14041 ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta
    14101 agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc
    14161 ttcgtttacg tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg
    14221 ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg
    14281 atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt
    14341 tctctttgtt ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg
    14401 tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat
    14461 ccatgacctt aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat
    14521 caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca
    14581 tcaaatcacg gtagtgacgc gtaacccaca gaaagccagt tccgttctcg gccctcgggt
    14641 gacgctatgg caagggctag ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa
    14701 cctggccgga gaaccgattg ctgataaacg ctggactcac gagcaaaaag agcgtctctg
    14761 ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc
    14821 gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt
    14881 ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga
    14941 agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt
    15001 ggtactggca ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct
    15061 gggcgggccg attggttccg gtcggcagta tctggcctgg attcatattg atgatatggt
    15121 caacggcatt ctctggctgc tggataacga cctgcgcggg ccatttaata tggtttcgcc
    15181 ctacccggta cgcaatgaac aatttgccca tgcgctcggt caagcgctgc atcgcccggc
    15241 cattttgcgc gtccccgcaa ccgccattcg gctgttaatg ggcgaatctt caatactggt
    15301 attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg
    15361 gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc
    15421 cgccagttaa ctcccggtgt tacaggatta gtggctttgc gcgataagat cgtctggtga
    15481 aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc
    15541 aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc
    15601 aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac caataaatgc
    15661 acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct
    15721 gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc
    15781 gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat
    15841 ctcgtcatcc ggcaccacgg taatatccac caacggaaac gctgatgaat atattttgcg
    15901 ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct
    15961 gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc ccgcatcaag
    16021 atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta attcttccag
    16081 cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg ttttcacaga
    16141 ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt ttgattctag
    16201 tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tatcaataaa
    16261 atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga
    16321 agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac gcgacaataa
    16381 ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta
    16441 tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat tccccatgaa
    16501 aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc ttaaggaact
    16561 gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc tcttcttcta
    16621 ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag cccatttcgt
    16681 gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata cgtaacactt
    16741 cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa acttccggtt
    16801 ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa agatgcaccg
    16861 gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc accgcccgct
    16921 cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca
    16981 gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca
    17041 gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg cgcaccagat
    17101 tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg aagttaatgc
    17161 agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg
    17221 cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt ttgtgcaaat
    17281 cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca
    17341 tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt
    17401 aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata
    17461 tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc agtagagtgc
    17521 agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa
    17581 ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc ttcaatttcc
    17641 ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta
    17701 agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt aaggaattcg
    17761 gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa ctgaacatac
    17821 agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta
    17881 ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa
    17941 ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc
    18001 ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacggagt
    18061 agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc aggtaaatca
    18121 cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc agttgggtgg
    18181 ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct
    18241 gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatccgcc
    18301 gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt cctttcggca
    18361 ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag
    18421 tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca
    18481 ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaacact aagtccggca
    18541 cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga ttttgcgaga
    18601 gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag ctgatagcca
    18661 gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc
    18721 ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag
    18781 cgggctttct ttcaccgatg gcgattagcc accataaaca tcaaaatcga agtacttttt
    18841 cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcttc
    18901 gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc
    18961 atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt tgaggaaacc
    19021 ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga
    19081 ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg cccaatgttc
    19141 attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc ctttcagcga
    19201 ctcgactgtc ggctgaatgt cagaattttt cgccaccacc aaacgagaat cggcagcgta
    19261 cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa gcgatgacat
    19321 gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg gattttcgac
    19381 aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga tatcgaagcc
    19441 aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat
    19501 gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg ccagaaccag
    19561 agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa
    19621 tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatattcc
    19681 gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac
    19741 gatagtgcaa caaacctagc gtgcaccatg atgatcattc catcaggtac agcttcccag
    19801 cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc ttgtcgtagg
    19861 tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat
    19921 ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc
    19981 cagcaaaggc gaaagcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt
    20041 catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt
    20101 tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg taagcttcct
    20161 gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc gttggctgaa
    20221 tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg tcggagaagg
    20281 caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg tcgatttttt
    20341 tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta catttcacct
    20401 gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa tcacctttag
    20461 catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc gtctccggta
    20521 gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga gcgagaatcg
    20581 acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg
    20641 tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt aacattcaac
    20701 gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag caggtatagc
    20761 ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg
    20821 ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag
    20881 agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt ctgatttatc
    20941 acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca
    21001 ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg tgtttcacgc
    21061 acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag
    21121 ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg taaaatcacc
    21181 atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg cgcatcgtgc
    21241 gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc
    21301 tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc gcgcagaacc
    21361 tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt
    21421 ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc ggcgggagtt
    21481 gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt tatgcatttc
    21541 gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac gtaacgtatc
    21601 gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga
    21661 gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca gatcgttcag
    21721 atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa cttcgcgacc
    21781 gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat
    21841 ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat
    21901 aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga cgtttttatc
    21961 gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac gcagctgctg
    22021 gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg
    22081 tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg ggatcgggat
    22141 caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc
    22201 catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg ggcgagcaaa
    22261 gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa
    22321 caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc
    22381 aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg tacggttctc
    22441 gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac
    22501 cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta agcggtttgt
    22561 ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga agttgtccag
    22621 ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg
    22681 tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc
    22741 aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg cgctggagct
    22801 gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg
    22861 gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt
    22921 ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc
    22981 atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg
    23041 tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga
    23101 gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga tcagctgtga
    23161 tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga agagaatggc
    23221 ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc
    23281 tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag caccaacaat
    23341 cagggtagcg ataaacagta ccgcgatggc aattccattt cgaaccagtt cgtcttcaaa
    23401 gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc
    23461 ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga
    23521 aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta
    23581 cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat
    23641 ttccttccca cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt
    23701 atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta
    23761 cggttctaac actgcccagg cctgataaga cgcgccagcg tcgcatcagg caagactgta
    23821 ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct tcaactcacc
    23881 cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac gggtaatttt
    23941 accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc
    24001 gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa cataggcttt
    24061 acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg gcgcaggtgg
    24121 tgcttcaacc ttcggtttag gcggctccac cggtttcggc ttcggtgggg cgaccggtgc
    24181 aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga gcgacggtgc
    24241 tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc
    24301 ctgggtggcg gctggcatca tatcaggctc atcgcgatcg cccgcttttg gaaccagcgg
    24361 gatagccgca aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac
    24421 aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa acttacttgc
    24481 caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac
    24541 acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa
    24601 cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc
    24661 gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag
    24721 ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg
    24781 gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa
    24841 aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa
    24901 tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt gccgccagcg
    24961 ctgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc gtgccgtgag
    25021 catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt
    25081 gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt tccggctcac
    25141 cgacaattgc cggttttgcg ctgcgaaaga tgcctgcttt ctcgcgacca atactttcgc
    25201 gatctggtcc cagccagtcg gtatgatcca gcgcaatgct ggttactaca gccacatcgg
    25261 catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga atcactacgt
    25321 caagttgtgc ctgtttgaac agccacaacg ccgacagcgt accgtactcg aaataggtca
    25381 gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg
    25441 gcaattcctg cccctgcaca cgtacgcgct cggtataacg caccagatgc ggtgaactgt
    25501 agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc
    25561 ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg acgccaagac
    25621 gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg tgcaggtttt
    25681 ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca
    25741 tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc
    25801 ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact acgccttcac
    25861 gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt
    25921 tcaggcgcat ttccggacga tggacgatca tgtcgatggc gcctttctcg atcaggaatt
    25981 cactgcgctg gaatcctggc ggcagttttt cgcgaacggt ctgttcgata acacgcggac
    26041 cggcaaagcc aattaacgct ttcggttcag cgatgttgag atcgcccagc atggcgaaac
    26101 ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct
    26161 cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt
    26221 cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta tcttccagcg
    26281 cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa ccgcccataa
    26341 aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacca
    26401 ccagcgcatc tttttcgccg gtttctttct gcgcagaagc cagacggtct ttatacttct
    26461 tggagtcacg aaacttcagc acatctttcg gctcaagctc gctacccagc tccacaaggc
    26521 ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac
    26581 acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc
    26641 tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc
    26701 ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg ttcgatgcca
    26761 gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac
    26821 attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg gagttacttt
    26881 ttattttgcg gcgcgtgccg cagcgcgttt gtgacgaata aattcgaaaa ccccaggcaa
    26941 tattgaaaca aaaataatgc caacaatcat cagcttaagg ttgctctgaa tgaaggggag
    27001 cgtgccgaag aaataacccg cgtaggtaaa aatcagtacc cacaacagtg cgccgatcac
    27061 gttataagcg gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggcgcgaa
    27121 cgttctgacg atcggtacaa aacgggcgag aataatcgtt ttgccgccat gtctctcgta
    27181 aaactgatga gtcttatcaa gataactgcg acgaaagatt ttcgaatccg ggttactgaa
    27241 cagtttgtca ccaaataacc gtccaatggt atagtttact gcatcaccga caatagcggc
    27301 aatcaacata agcaccacca tcatatggac attgagatcg ttagtttcca gcgacgccaa
    27361 tgcaccagca acaaacagca gcgaatcacc aggcaggaat ggcgttacca ccagccccgt
    27421 ttcgcagaac agaatcaaaa acagaatggc ataaacccag acgccgtact ccgcgaccag
    27481 ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat
    27541 atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc tacagcctga
    27601 aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg
    27661 gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc gcttttgccg
    27721 ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccat ctctccggct
    27781 ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa
    27841 aggcattcgc tttgatatct accaccacat aaggaccgtg acgcgtgaca ttaatatgca
    27901 taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag gtgaaatcat
    27961 tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa
    28021 aatgggttac ccctttactc aataccgccg ggcgcagccg atgattgtag atgatgtagc
    28081 gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttaaccc
    28141 aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac gccgcgtctt
    28201 tgcgctgcgc ggttgtttcg aaatgcacca cctgcccggt accgtgtacc cctgcgtcag
    28261 tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc gccttttcca
    28321 gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac ttactgccgt
    28381 cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg gacattagta
    28441 cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg
    28501 aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt cattacgcac
    28561 gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg ggaattcgtt
    28621 ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt cttctgccgc
    28681 cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg
    28741 gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac
    28801 gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca gcatattgaa
    28861 cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga gcaatttcgc
    28921 actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg aaatcaggct
    28981 ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg ccgccagcag
    29041 ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac
    29101 aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct
    29161 gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag cggtagcttc
    29221 tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg
    29281 cacagtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag
    29341 tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc
    29401 cacagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc
    29461 cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt
    29521 gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca
    29581 tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt gcgcagttta
    29641 tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca
    29701 tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcgggccatg cagcgtaatg
    29761 cgaccaaact ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc atgcccaata
    29821 aacttgctat aggcttcaaa cacttgcgta gtaccgcgtg ctttaccttc cagggtataa
    29881 cctgcgatat gcggcgtgcc gatatccact tttgtcagca actccacgtt aagttccggt
    29941 tcgccttccc agacatccag cactacgctt aacttctggc cttcattcag gcaggtcagc
    30001 aacgcagtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc tcccggcttc
    30061 aggctacgga tcagtttttc atccgccaga tgcagcgttt tgtacggccc atctttgaag
    30121 agtggcgtat ggaaagtcag aatatccgca tgctgaacta attcatccag cgagcggaaa
    30181 tcaccctcat ccccacggtc agcgcgaggc ggatcgcaaa gcaatgtttt aatccctaac
    30241 gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat ccccaccgta
    30301 cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gtagtgagga gaaaacatat
    30361 tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc cgcctgcttc
    30421 aaccatgctt catcgacatg gtcggtcccc gcagtggcag tgccaacaaa tttaatgggt
    30481 tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag cgcatccgcg
    30541 tctgccagtt gagcgacggg gattggacgc ccgggaaccg cggtcacctc acccaaacgg
    30601 ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta
    30661 cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc aggcatactt
    30721 gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc ctcctcctgg
    30781 gcaaccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc ctttatccag
    30841 tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga cgcaacaaca
    30901 aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct
    30961 gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc tggccgcgca
    31021 aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa
    31081 taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca
    31141 gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat
    31201 tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc
    31261 gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca aacagatctg
    31321 gcaatcgatg ctggaacttt ccggggtgaa cgatggcgag ttaattccag cgaaactgtt
    31381 taaccatctg gtgacctggt tgcaggcgcg ccagacgtta agccagcaaa atacgccgac
    31441 gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcagcgtt
    31501 atcggcgtat atccagcaaa aatatggtct ttctgcgcaa tcatcgcttt cttctgccca
    31561 ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg atccgcgtga
    31621 tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac
    31681 gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg
    31741 ttagccccga cgaaatgaca gtatcgtgac aataattccc agcaccgcag agatcgcccc
    31801 ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca gcggcccgga
    31861 gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg
    31921 tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc
    31981 tccggttaac gccgcgcccg ctaatgcgac ccacgcacct ggggcttgcc agagcagcaa
    32041 caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc caaaacggtc
    32101 cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaact cggtaagagt
    32161 aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag tcccgataac
    32221 cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa acccgacaac
    32281 gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca
    32341 ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg
    32401 gctatgaatc aacaggccaa gcggagcacc aacagcgagg gcaccgtaaa tcgccattcc
    32461 attccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc cccaggtcag
    32521 agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga tgaccaacag
    32581 ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg cgccgccagc
    32641 cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt gatcggccag
    32701 tcgcccggca tagccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatgcc
    32761 gaccatggta ttgccatagc ccagttcatg atgaacaaac agcgggataa ccggcaacgg
    32821 cagccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt
    32881 ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg
    32941 caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg
    33001 atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctggaactg
    33061 gtggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg
    33121 gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc
    33181 ggcctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg gtgccgccga
    33241 agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg gtcacgatgt
    33301 tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg ataaagccgt
    33361 gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc agtgagtgac
    33421 cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact tcacggatag
    33481 ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc aggtaatcga
    33541 ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct tcgccagacg
    33601 gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca gcatagatgt
    33661 gagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg ccgccgccag
    33721 cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt
    33781 tgtatttagt agacagcgca cccatcgcgt cgaattcgca ggccatttcc cagcacagct
    33841 cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc tctactgcgt
    33901 taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta acgccatgaa
    33961 ttttaaacgg cgtggcgagg caggcagaaa cgccggatgc catcgctttg gtgaccacat
    34021 acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg aatacctgga
    34081 aacgcgggga accgccgcca gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag
    34141 cttccggaga aaggcccgca tctgcgattg cctgctccat agaaaggaat gcataaatgg
    34201 atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta tccagtttta
    34261 cgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag aaagtgatcc
    34321 ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg atgctggaaa
    34381 caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag tcgcacatag
    34441 agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg atcagccatt
    34501 taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc gcctgtaaga
    34561 cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt taatgctgag
    34621 ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa cgggctggaa
    34681 gagacgcgtt atgtttttct gggaggcaac caattagagg tacgctttcc tgagcatcca
    34741 catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt cctgacgcta
    34801 tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca acgcttacat
    34861 ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc gcatcaacac
    34921 tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat gcccttgccc
    34981 ggttgccatc gtttattgct caatgaaggc cgcgtgacgc tggatttatg gtttggcgat
    35041 attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga tgcctggttt
    35101 ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct gtttaacgcc
    35161 atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc cggttttgtc
    35221 cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa
    35281 cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc cacgccgtgg
    35341 tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg tattgccagc
    35401 gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta ttgcgcggat
    35461 gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc gttattaagc
    35521 aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc tcgtcggttt
    35581 tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca gttaggctgg
    35641 gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc cgcagaactg
    35701 gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac aaattgcagc
    35761 ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg taatgtgctg
    35821 gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca gaatttatcc
    35881 cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac acacagcgta
    35941 gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac tctcccggtg
    36001 tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc agagctgaag
    36061 caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca acatcattgt
    36121 attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga cgatcagcag
    36181 cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa agaggttgat
    36241 gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg
    36301 gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt ggcggagcag
    36361 aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc ggctttaggt
    36421 tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca gatgagcgac
    36481 gaaccgattc cgatggatgc cagtacgctg acggcgttaa acccgaatcg gttatgggtg
    36541 cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc agtgtttgcc
    36601 ggatgcggcg tgaacgcctt atccgaccta cgtgtgacgt gtaggcctga taagacgcga
    36661 taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tactgctgtg acgcctgctg
    36721 aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccggc
    36781 accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc
    36841 atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc
    36901 aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca gaatgcgtga
    36961 ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcaccggc ttcagttgag
    37021 cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag
    37081 tgctttaatg aagcgtgccg gacgctcggg aatgccattt gtcagataat ccatcacctg
    37141 cgcatgtacc cgccgttgaa acacaacacg atccggttcg aagtcgcctt ccagattgtc
    37201 gcaactgacg ttaaacggat atcccgccgc cacacagaac aaccagtcga gtgcctgcgg
    37261 cttcacttca acatcttcaa actggctttg cgtttgggca tcacgcccat ccgggcaata
    37321 ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc agtgcgaaat
    37381 ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca attcggttat acggtacttc
    37441 cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg tattaaattc
    37501 atcggcaaag cagctattaa aaatttcaat caactgctca tagtggtgtg tactgttcat
    37561 tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag taatttggca
    37621 ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc tttgctcaac
    37681 accagccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac aaagcccgtc
    37741 gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt tgacgttgcg
    37801 ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag cgtaacgaag
    37861 gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa accgacgcag
    37921 ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc ttcttcaccc
    37981 agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg caaaatctgc
    38041 cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga gccgacaaag
    38101 gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag
    38161 atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcgttagcg
    38221 ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc accggcaatc
    38281 gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggggaa
    38341 accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc
    38401 aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttagag
    38461 gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt aaaggttgat
    38521 cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc gcgcgatgct
    38581 ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt
    38641 gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg gcagcgagtt
    38701 tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg
    38761 aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca
    38821 gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac
    38881 gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca
    38941 cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa tagcgacgct
    39001 gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca gcgccgacaa
    39061 tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt
    39121 tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc agcgcaatcg
    39181 cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc
    39241 gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcactg
    39301 cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact tcttcgccaa
    39361 agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatgag
    39421 caatgatttg ctgcccgctg ctgataccgc cgagaatgcc gcccgcatgg ttgctctgga
    39481 aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact acgtcaaaac
    39541 cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg
    39601 catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg gcaacaacgg
    39661 tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg
    39721 cgtcgatttt gtccgggtcc gggcagaaga acggattttg ttcaaccagc gaccagtctt
    39781 tgatttccag cggaatgtcg cccatctggg tcaagcagcc gcggatttca ataccaaatt
    39841 tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg
    39901 cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat
    39961 cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta atcctgagag cgctggtcag
    40021 tgttttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa acaccggaga
    40081 gaattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac
    40141 gacggtcgag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat
    40201 cgacgatgca gccgagcgcc agcccatgcg attcgccgaa ggtggttacg cgaaagagtt
    40261 gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa
    40321 tctttataaa tcgcgaaata ttcacgggcc gccagcagct gctctttggt gagcataaac
    40381 acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatccgg atattgttcc
    40441 ataagatgta ccatgctgtt gccgacttca caaatcaaca cgccatcatc agcaaggtaa
    40501 tctgccgcgt ttccaagaat gcgacgcgtc agtttaaggc cgtcggtgcc agatgccagg
    40561 cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcgtcgaca
    40621 tacggcgggt tagtgacaat cagatcgtac tgcactttcg gcaaatcacg gaacagatcg
    40681 gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc
    40741 gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa ggcataagca
    40801 caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg
    40861 ctaatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg cggcaccagc
    40921 acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt caggtaagcc
    40981 accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg tttttcgctg
    41041 gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta gagcgatggt
    41101 aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt
    41161 gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg cagctcattt
    41221 actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt
    41281 cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga
    41341 aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa
    41401 atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc
    41461 gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac cgaccgcagc
    41521 gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc
    41581 attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg aaatatgttc
    41641 gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat tcaccggaat
    41701 tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga
    41761 ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg cacgggaagc
    41821 atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg atggcatttc
    41881 accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt gaagtggaag
    41941 agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca gcgtaaacgc
    42001 cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc gtaggcctga
    42061 taagacgcat cagagcaata ctacgaacta cagttcattc aatacatact gaaaacgaca
    42121 cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat cgtgtagtac
    42181 tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat cacaaccgta
    42241 agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc catcattatc
    42301 agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg aaagcgtggt
    42361 ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga cataattttc
    42421 actccaggag atatcgtatg aagtgaccgg tatcgttggg catattgtgt tatgcactgt
    42481 aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg atgcgatcca
    42541 agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg cacaacgaaa
    42601 aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt cagtcagtac
    42661 caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat cgcctttata
    42721 cgcaaaagaa tcatatttcc catatgtaac ctcctttggc gttatcaccg taacattgaa
    42781 aacactgctc gctacagcac cggttacctt gtccatggca taataaactc tcacactgtc
    42841 aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga caacactcag
    42901 aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag aaatactcaa
    42961 aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg ctctttgcct
    43021 gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc gtgacacctg
    43081 ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc ccagcgccgg
    43141 atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct tctgttcctg
    43201 tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat tgaaacacca
    43261 atggcactcg ctctgactgc ccaaggttca ttaaatcgct ggcggcaatg gcaggaaaat
    43321 gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg attaaattgc
    43381 cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac atcaggctac
    43441 taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg tggcccaggc
    43501 ctcaaagtcc gcctctggca gaacgacacc actctttttt actggcacag ccgttaacac
    43561 cggtaaatcg gtttcattca ccataagtgg cgtattgatc gtaaaactct gccctgcctg
    43621 tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat caaaatcgct
    43681 tagtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg ccgtatcgca
    43741 ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat caataagcac
    43801 atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag gcggctgcac
    43861 taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc acagcaaaat
    43921 aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt aaattcagct
    43981 gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg aactcccgag
    44041 gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg atcactactt
    44101 ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc accgttaatc
    44161 acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt gttacaaaca
    44221 agcgagtagt caatgggacg tttataatca accccattga tcttattaat gaatacatta
    44281 ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac aagcacccgt
    44341 aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac acaacatatc
    44401 agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct cccggcctca
    44461 gcctttgcgg ctctgctctt catcgacctg gcaactgtta ccgttgcaag tgaaaaagag
    44521 cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac ccatcttcac
    44581 attcagcggt gcagaggttt tcggcgacat cactattggc gagaaaccgg ctgcaggatt
    44641 gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacggcgtcg ggttattgac
    44701 ggtaaattcc tgaccgttgc gggttaaagt aaccttatat tgccaggggt ttttcatgct
    44761 gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta gtgctatttg
    44821 cagcgtgttg gctttgtttg attttggcgg gatctcacgc acgttgaagt agaacaggct
    44881 ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta cctgcccgtt
    44941 catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac tgatcttgtt
    45001 acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat cgttattgcg
    45061 caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac gatctggcgt
    45121 tacagaagcc tgggcgacaa actagcagcg ctaagcagta acgctagcgt cgtagcaccc
    45181 aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc
    45241 tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg
    45301 ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa
    45361 gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc
    45421 actccagaac acactcatgt gttcgccagg tttcacccca gccagataaa cattgccgtc
    45481 atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg
    45541 atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa
    45601 tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc
    45661 ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac
    45721 gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac
    45781 cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattctgggt
    45841 acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc
    45901 agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc
    45961 atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata
    46021 gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc
    46081 atagttaccg ttatagctaa cggtactgtt gtcaccccac ggcatactga gcgaaatgta
    46141 catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg agatgctgac
    46201 attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt ttgtctgctc
    46261 ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac ccgcatcgcg
    46321 gaagttctgg ttataagtgg cagtgtacat ctctttgtcg ttgcccgtgc gtaccattcc
    46381 gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg agaagcgata
    46441 tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac tcacacggaa
    46501 ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt tggtgtgcga
    46561 gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga cgccaagcgc
    46621 cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc cgttagcgat
    46681 cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc cccactcttg
    46741 cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt atggcatcaa
    46801 ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc gaatatgcaa
    46861 cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg gaacctgcgt
    46921 ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt gtgccacgcc
    46981 ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt cagtactgac
    47041 actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat cctcgcccag
    47101 cgccagtttg gcttttaatg atggtaacgc ccgctaggca tagtagcgac tccactccca
    47161 ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata
    47221 gttagtctgc cagtcagcac gcatacgcca cggccccagg ttaaccccga ccgtcccgtt
    47281 gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat
    47341 gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatcccagtc
    47401 gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa
    47461 atcagcctta atttccatgc cttccagttg accggatttc aggcatttag catcgtggct
    47521 ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt
    47581 cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc
    47641 ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata
    47701 tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt ccagaaaacg
    47761 ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag
    47821 cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc
    47881 tatccgtttt tattcttgtt cggtcaaaat ccatttaaat cccccatagc agtgtcgcta
    47941 tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcga aattacccag
    48001 atctggcgca tcagctgcgc cgaccagcca ggctttaaag ttcagtgtct gcttcgcttt
    48061 gcctttattg gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta
    48121 agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg cacaggtatc
    48181 ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt
    48241 agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt
    48301 cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc
    48361 cagtttgtga tcgtccggaa cgatagaaca cactgagtct tcaatggtgc cgtaaaaacg
    48421 tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat
    48481 tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa ctataaataa
    48541 ttaaa
//
LOCUS       DAZPLM010000007        46785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPLM010000007 DAZPLM010000000
VERSION     DAZPLM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.007"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHVC7_000334"
                     /gene_synonym="thiJ"
     CDS             complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHVC7_000334"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR2114484.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            972..1340
                     /locus_tag="ACHVC7_000335"
     CDS             972..1340
                     /locus_tag="ACHVC7_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR2114485.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHVC7_000336"
     CDS             complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHVC7_000336"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR2114486.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHVC7_000337"
     CDS             complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHVC7_000337"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR2114487.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHVC7_000338"
     CDS             complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHVC7_000338"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR2114488.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHVC7_000339"
     CDS             complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHVC7_000339"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR2114489.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHVC7_000340"
     CDS             complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHVC7_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR2114490.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHVC7_000341"
     CDS             complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHVC7_000341"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR2114491.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHVC7_000342"
     CDS             complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHVC7_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR2114492.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8311..9207
                     /gene="murQ"
                     /locus_tag="ACHVC7_000343"
     CDS             8311..9207
                     /gene="murQ"
                     /locus_tag="ACHVC7_000343"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR2114493.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9211..10635
                     /gene="murP"
                     /locus_tag="ACHVC7_000344"
                     /pseudo
     CDS             9211..10635
                     /gene="murP"
                     /locus_tag="ACHVC7_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHVC7_000345"
     CDS             10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHVC7_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR2114494.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHVC7_000346"
     CDS             complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHVC7_000346"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR2114495.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12997..13572)
                     /locus_tag="ACHVC7_000347"
     CDS             complement(12997..13572)
                     /locus_tag="ACHVC7_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR2114496.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13633..14082)
                     /locus_tag="ACHVC7_000348"
     CDS             complement(13633..14082)
                     /locus_tag="ACHVC7_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR2114497.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14069..14494)
                     /locus_tag="ACHVC7_000349"
     CDS             complement(14069..14494)
                     /locus_tag="ACHVC7_000349"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR2114498.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14708..15577
                     /gene="amiA"
                     /locus_tag="ACHVC7_000350"
     CDS             14708..15577
                     /gene="amiA"
                     /locus_tag="ACHVC7_000350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR2114499.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15581..16480
                     /gene="hemF"
                     /locus_tag="ACHVC7_000351"
     CDS             15581..16480
                     /gene="hemF"
                     /locus_tag="ACHVC7_000351"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR2114500.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHVC7_000352"
     CDS             complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHVC7_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR2114501.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHVC7_000353"
     CDS             complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHVC7_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR2114502.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHVC7_000354"
     CDS             complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHVC7_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR2114503.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHVC7_000355"
     CDS             complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHVC7_000355"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR2114504.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHVC7_000356"
     CDS             complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHVC7_000356"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR2114505.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHVC7_000357"
     CDS             complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHVC7_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR2114506.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHVC7_000358"
     CDS             complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHVC7_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR2114507.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHVC7_000359"
     CDS             complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHVC7_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR2114508.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHVC7_000360"
     CDS             complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHVC7_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR2114509.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26014..27417)
                     /locus_tag="ACHVC7_000361"
     CDS             complement(26014..27417)
                     /locus_tag="ACHVC7_000361"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR2114510.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHVC7_000362"
     CDS             complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHVC7_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR2114511.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHVC7_000363"
     CDS             complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHVC7_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR2114512.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHVC7_000364"
     CDS             complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHVC7_000364"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR2114513.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHVC7_000365"
     CDS             complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHVC7_000365"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR2114514.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHVC7_000366"
     CDS             complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHVC7_000366"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR2114515.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHVC7_000367"
     CDS             complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHVC7_000367"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR2114516.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHVC7_000368"
     CDS             complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHVC7_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR2114517.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHVC7_000369"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHVC7_000369"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR2114518.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="ACHVC7_000370"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="ACHVC7_000370"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR2114519.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="ACHVC7_000371"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="ACHVC7_000371"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR2114520.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="ACHVC7_000372"
     CDS             complement(37402..38445)
                     /locus_tag="ACHVC7_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR2114521.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHVC7_000373"
                     /pseudo
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHVC7_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHVC7_000374"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHVC7_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR2114522.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="ACHVC7_000375"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="ACHVC7_000375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR2114523.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="ACHVC7_000376"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="ACHVC7_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR2114524.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHVC7_000377"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHVC7_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR2114525.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10713 a        13154 c        12119 g        10799 t
ORIGIN      
        1 catcggcgcc attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg
       61 ctgctgggtg tagcgcatca cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt
      121 taacgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
      181 tttcaggtcg gtttttaccc gtgaatggga aatgacattc acgctgtcgg cactgaccac
      241 caccacctgc atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt
      301 taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt
      361 tttaccggtc aggatttcca gctcaaagat attgggggta attccttgcg ccagcggcag
      421 taaatattgt cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc
      481 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
      541 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
      601 aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa tagccgctaa accattcgtc
      661 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
      721 cacggcaaag acattcaagc cgttctgttt tatagcaggc accgcaatgc tgttgcctac
      781 gctgccgtac actacctgcg actgcacggc gacgatatcc gcctgcagcg ccctactctt
      841 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
      901 tattccaacc gaaactttac ctgattctgg cagtcaaatc ggctatcaca aaacaaggat
      961 aaggtaattc aatgaagaaa atcatttgtc tggtcattac actactaatg acactccccg
     1021 cttacgcgaa gttaactgtt catgaagaag cccgcatcaa cgccatgctg gagggattag
     1081 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
     1141 tttctcatct gcgtctgaag ctcggcaata cccgtaaccg cattgacact gccgagcagt
     1201 ttattgataa ggttgcttcg tcgtcatcga ttaccgggaa gccgtatatt gtgaagatcc
     1261 ccggtaagag cgatgagaac gcacagcctt ttttacatgc cttaattgcg cagacggata
     1321 aaacggtgcc tgcgcaataa tcttaaatcc ccgccccctg actaaaatgc tcttccccaa
     1381 acaccccggt ggaaaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac
     1441 cagggttagc ttttgccacc cgcagtgctc cggcaaccgc gccgccggag ctgacgccac
     1501 agaatattcc ttcccgcact gccagttcgc gcatggtgtt ttctgcatcg cgctgatgaa
     1561 tatccagcac ctcatccacc agagaagcgt tgaaaatccc cggcagatat tccgcaggcc
     1621 agcggcgaat gccgggaata ctgctgcctt cttccggctg caggccaaca atcgttactg
     1681 gtttggattg ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc
     1741 tggagacaaa atgggtaatg cgcccgccgg tttgctgcca gatttccggc ccggtagtgg
     1801 tgtaatgggc gtaagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac
     1861 gattcgccat ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa
     1921 gaatcagttc cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
     1981 gcatcagcaa tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg
     2041 tgttaccact ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg
     2101 cctcgacgat catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt
     2161 ccagttttaa ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag
     2221 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tatccatacg tggtgttgcc
     2281 tgatgcgacg cttgcgcgtc ttatcaggtc tacaggttac aaaccttgcc attaaaaagc
     2341 ccggatcgcg ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt
     2401 gcgagagcaa gttcctcatc gcgggtttcg atacgctcgt cgccgttata cagccgcgca
     2461 tgttgcagac caacgaataa acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg
     2521 accgtcagcg gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct
     2581 ttcgggctgg cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg
     2641 atatccactt cccacgggcg caggaagaga tccaccggcc cctgataagc aggtgtgtag
     2701 cccagcggcc agcgatgtgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg
     2761 cggttcactt cgcccataaa ttcgagcaca aaacgggtcg ctggttcgcg ccatacctgc
     2821 tccggcgcat cagcctgttc aatattgccc tggctcatca ccactacacg atcagctact
     2881 tccgtcgctt cttcctggtc gtgggtcacg aacacgctgg tgaatttcag ctcttcatgc
     2941 agttgacgca gccagcgacg cagctcttta cgcacctgcg catccagcgc gccaaacggt
     3001 tcatcaagca gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacgcgctgt
     3061 ttctggccgc cggaaagctg tgccggataa cgatccgcca gatgagcaag ctgcaccatc
     3121 tccagcaatt ttgtcacttt cgctttgatg gctgcggcat tcgggcgatc gcgacgcggc
     3181 agcaccgtca ggccaaaagc gatattgtcg aataccgtca tatggcggaa cagcgcgtaa
     3241 tgctggaaca cgaaaccgac cttgcgatcg cgcgcgtgca ggcggctgac gtcggtgccg
     3301 tggaagcgaa tatgcccgct ggtttgatgc tccagcccgg cgataatgcg cagcagcgtg
     3361 gttttcccgg aaccggacgg ccccagcaac gcgaccatct gacctgaagg aatatccagt
     3421 gagatatcgt tcagcacctg ggtgcgacca aacgacttct taatattggc aatctcaatg
     3481 ctcatgatgt tcctcctgct gtgcgcgttt ttcctgattc tccaggcgcc actgcaacat
     3541 actttttaaa aacagggtga taatcgccat cagcgttaac agtgccgcag cggtaaagga
     3601 gccgacggtg ttgtagtcct gctccagcaa ttcaatctgt aacggcagcg acagggtttc
     3661 gccgcgaatc gagccggaaa ccaccgacac cgcgccaaac tcgccaattg cgcgggcgtt
     3721 ggtcaacacc acgccataaa gcagcgccca gcgaatgttc ggcaatgtga cgcggcgaaa
     3781 catctgccag ccagacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct
     3841 taacatcacc ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag
     3901 caccattccg ggccaggaga acataatttg cagattatgt tcatccagcc agccgcccag
     3961 cgggccgtta gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacggc
     4021 aaacggaatg tccagtagcg tcagcagtaa ctggcgtcca gggaagttaa agcgcgtcac
     4081 cagccaggcc agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat
     4141 caccgtcagc cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat
     4201 cagccctttg ctgaacgcct gtacgaagat ataaatcatc ggcaccagca ggatgaacgc
     4261 cgataccagc atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc
     4321 ataacgcttc aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga
     4381 ctttgcagag tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca
     4441 atcgcgctcg ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc
     4501 acttccgtct tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga
     4561 ctacgggtaa acgacagcgc cacgcccgcc accagcgccg gagaaagttc cggcagcacc
     4621 actttgcaga aactctgcca gcgcgttgcg ccaagcgttt ctgccgcttc ttcatattcc
     4681 gggcctaact cttccagcac cggctgcacg gtacgtacca caaacggaat gctggtaaag
     4741 gccatcgcca ccgcaatccc cagccaggta taggtgactt tgatatcaaa cttcgccagc
     4801 cattcaccgt aaaaaccgtt tacagaaaag agcgaggcta gcgttaaacc ggcgactgcc
     4861 gttggcagcg caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga
     4921 tagcgggtta agatccacgc catcagcagg ccaaaaacgc cgttaaaaat cgatgccaca
     4981 aacgctgaca gcagcgttac tttgtaggcc gctactacct gcgggttggt gatcacctcc
     5041 cagtactgcg cccagctcat ctgagccagc tgcatcacca gcgcggagag cggcagcagc
     5101 aaaatcaggc acacaaacag cagactggtg ccgaggctta aggtaaagcc cggcagcacg
     5161 cgtcttgatg aaactgcaaa cattaattac gccccgccgc taacagcttg tctaactcgc
     5221 cgccgctggt gaagtgggtt ttcatcactt ccggccagga gccaaatttg tcttccacgc
     5281 ggaacagctc ggtctgcggg aatttgtctt tcagtttatc catgacttcc gggttattca
     5341 cgcggtagta atagtcggtg atgatggttt gcgcctgcgg gctgtagagc cagttaaggt
     5401 aggctttggc ggctttttcc gtaccgttgg cctgcacgtt tttatcaacc cacgccaccg
     5461 ggaattccgc cagaatgttg gttttcggaa tcaccacttc aaaaccctgc gcttcatact
     5521 gtttacggat gttgttcact tccgactcga agctgatcag cacatcgccc aggccgcgct
     5581 cggcaaaagt ggtggtcgcg ccacgaccgc cagtatcgaa cacttcaacg tttttcagga
     5641 actgggtcat aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc
     5701 cccatgccgc cagataggtg taacgcgcgt tacccgacgt tttcgggttc gggaaaatca
     5761 gcttcacatc ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac
     5821 gcaccaggaa gcccatggtg gagtagaatg gcgagctatt atttggcagg cgcgtttgcc
     5881 agtccgccgg gatcagcttg cctttgtcat gcaggatttg tacgtcagtc acctggttat
     5941 aagtgacaac gtctgctttt aagccctgca aaattgccag cgcctgtttt gatgacccgg
     6001 catgagattg ttttattgtc agtttgtcgc cgccgttttc tttggtccat tgttgctcaa
     6061 acggcgggtt cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt
     6121 ccgttgcctg tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt
     6181 tcagtaagtt aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca
     6241 tatttataac ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt
     6301 gaaaagggaa taagaaccgg gtttaaaacg aaatcctccc cacaaatgca gggaggaaac
     6361 agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac
     6421 attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc
     6481 gacttccagc ggatcggcga ggcgacgcag cgggattgct ttcgccattt cagtcagcac
     6541 cgactctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg gtgtgcgcac
     6601 gtatcccggg caaatggcgt taacgcgaat accagactgc gcgtactcca ccgccagcga
     6661 ttttgtcagg ccaacaatcg ccgctttcgt taaggcgtac gccgtttcgc caggatcggc
     6721 caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc gggcaatcat
     6781 ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatgt caatatggaa
     6841 atcgcgatcg tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt
     6901 caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga
     6961 cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc
     7021 gtccgccagc ttttcgatct caggggagat atccagcaag attaggtttg cgccatgacg
     7081 cgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt
     7141 cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca
     7201 ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga
     7261 aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg
     7321 cgctgaatca ttttcaatga ctccacatca ttgagttgca caagaccaac aaacagtaaa
     7381 tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca ttcggtttca
     7441 ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc agccagagaa
     7501 gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa gacaatctct
     7561 tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa
     7621 acggtggctt gtacgtgtgt gtcggcttca taggccacgc gatagccaat tttcatcagt
     7681 ttgaacgaca gatcgcgccc caccagcgcc gacccgccca ggcccgttat ctggataaac
     7741 ggtgctttac tgatgacctc aatgattttt tgcagccgag cgtagtcgaa caacgcacag
     7801 gtttgctcca gcgccagctc tttttcgcga ttcagtttgc gggcaatcac ttccagcgaa
     7861 tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg
     7921 gcactgtatt cgccaattaa cgccatacgc aattcagtaa atccttgcgc gccgagcttc
     7981 tgggcaaatt tcacaatgct cgactgactg atgccgagct gtttcgccat cttgcgcgaa
     8041 gagacggatt tcagctcact cacgttggcc cgcagaaagt cagcaatttt ttgctcgttc
     8101 tcggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt ccactctccc
     8161 gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc tgcaagtgta
     8221 gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac atctgcaatt
     8281 atttgatcgt aaatagtaag gtcaccaccg atgcaacttg aaaagatgat tactgaaggc
     8341 tcgaacaccg cctcggctga aattgaccgc gtatcgacgc tggaaatgtg ccggattatc
     8401 aacgatgaag ataaaaccgt accgcttgcc gttgagcgcg tcctgccgga tatcgccgcg
     8461 gcgatcgatg ttatccacgc ccaggtaagc ggcggcggtc gtctgattta tctcggtgcg
     8521 ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac ctacggcgtg
     8581 aaaccgggtc tggtggttgg tttgattgct ggcggcgaat atgccattca gcacgcggtg
     8641 gaaggcacgg aagatagccg agaaggcggc attaacgatc tgaaaaatat aaatttaacg
     8701 gcacaggatg tggtggtcgg tattgccgcc agcggtcgta cgccgtatgt gattgccgga
     8761 ctggaatacg cccgccagct cggttgccgc acagtgggaa tttcctgtaa tccggggagt
     8821 gccgtttcca ccaccgccga gtttgccatt acaccgattg taggtgccga agtggtcact
     8881 ggttcttcgc gaatgaaagc aggcacagcg cagaaactgg tgctcaatat gctttccacc
     8941 gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc
     9001 aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc
     9061 gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact gtaaaacggc cattgtgatg
     9121 gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt
     9181 attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag tgaacttctg
     9241 aacaccattc ttacccgtgt cggcggactg ggaaatatcg ccagttgtgg taactgtatg
     9301 acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccaatat caaaacgctg
     9361 gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt
     9421 aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg gcgaggcacc ggtacaggat
     9481 gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg
     9541 caacaatttc tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg ttttattgcc
     9601 gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt tccggcagat
     9661 gctcagggca cgctacccga cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc
     9721 actttcctgg tgattctggt gggctataac gccgcccagg catttggcgg cacgggcgtc
     9781 aatggcgcaa ttattgccgc actctttttg ctcggttata accccgctgc aaccaccggt
     9841 tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg caatattatc
     9901 ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg
     9961 tcggacgatc tcgacatgct gctgacctcg ttaatcaccc tgctgatcac cgctacgctt
    10021 gcgtacctga tcattatgcc gctgggtggc tggttattcg aaggtatgtc atggctgttt
    10081 atgcacctga acagtaatcc gttcggttgt gcggttttag ccgggctgtt cctgatcgcc
    10141 gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat ggacagccag
    10201 ggattcaaca gcttatttcc catcctttca atggcaggcg cgggccaggt gggcgcggcg
    10261 ctggcactct actggcgggc gcaaccgcac agtgcgctac gcagtcaggt acgcggggcg
    10321 attattcccg gcctgctggg cgttggtgaa ccgctgattt acggtgtcac cctgccccgc
    10381 atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg gcggtttgtt tatcggcctg
    10441 atagcctgat ggggtctgcc gatgggctta aacagcgcct ttgggccgtc tggtctggta
    10501 gcgctgccgc tgatgacttc cgcacaaggc atccttccgg ccatggcggt ttatgctggc
    10561 gggattctgg tggcatgggt ttgcgggttt attttcacca cgctctttgg ctgccgtaac
    10621 gtcaatctgg actgatatca tgaaacggac aatgctctat ctttctctgc tggctgttag
    10681 ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa agctcgccag agaaagcagg
    10741 gtttaacgtc gaacggctta accagatgga tcgctggatt agccagcaaa ttgatgtcgg
    10801 ttatcccggc gtaaacctgc tgatcattaa agataatcag attgtgtatc gcaaagcctg
    10861 gggagcggcg aaaaagtacg atggcagcgt gttgatggaa cagcctgtca aagccaccac
    10921 cgggacgctg tatgacctgg cctcaaacac caaaatgtac gccacgaact tcgccctgca
    10981 aaagctgatg agcgaaggca aactgcatcc tgacgatcgg attgcgaaat atattccggg
    11041 gtttgccgat agtccaaatg acaccattaa aggcaaaaac accctgcgga tttctgacct
    11101 gctgcatcac agcggcggtt tcccggcaga tccgcaatac ccgaataaag cggtcgcggg
    11161 cgcgttatat tcccaggata aaggccagac gctggagatg atcaagcgca cgccgctgga
    11221 atatcagccc ggcagcaaac atatctacag cgatgtcgat tatatgctgc ttggatttat
    11281 cgtcgagtcg gttaccggtc agccgcttga ccgctatgtt gaagagtcga tttatcgccc
    11341 gctcggcctg acacatacgg tatttaaccc gctgctgaaa ggctttaaac cgcaacaaat
    11401 tgccgccacg gaattgaacg gcaatacccg cgatggcgtg atccatttcc cgaatatccg
    11461 cacctccact ctctggggtc aggtgcacga tgaaaaagcc ttttattcga tgggcggcgt
    11521 ttccgggcac gcaggtttgt tttccaatac cggcgatatt gcggtgttaa tgcaaacgat
    11581 gctgaacggc gggggctatg gcgatgtgca gctgttcagt gcggaaacgg tgaagatgtt
    11641 caccaccagc tctaaggaag atgccacttt tggcctcggc tggcgcgtga atggtaatgc
    11701 caccatgacg ccgacgtttg gcacgctggc aagcccgcaa acctacggtc acactggctg
    11761 gaccgggaca gtaaccgtta tcgatccggt caatcatatg gcgattgtga tgttaagcaa
    11821 caagccccat tcgccagttg ccgatccaca aaaaaatccc aatatgttcg aaagcggtca
    11881 gttaccgatt gcaacttatg gttgggtagt tgatcaggtg tatgcggcgt tgaagcaaaa
    11941 gtaataaaaa ggccagtcag ttttgttact ggctggcctt taatcatcga atcagaaacg
    12001 cttacaacgc cattaacttg tccagcgacg gcgcgaagta atagccgccg gttaccggtt
    12061 tggtgaaacg caacatcgca tcacgcttac catcggtatc gccaaacatg ctcagcagtt
    12121 gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac
    12181 tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca
    12241 gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg
    12301 cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg ttgagctgct
    12361 tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggcatcc acgccgtctt
    12421 tgataaccgc cacttcgcga cgcgtctctt cacccgccgg gttttctgta ccgtcaacaa
    12481 agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct ttcacttcaa
    12541 tgcagtcacc aaaggcttcc atcgccgcct gggcgacaga gaagtttacg tcgtgacgca
    12601 gagaaagaat gtggatcaac acatcgaact gggtggtcgg cgcaaggcct ttaccgtagc
    12661 ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc caggtgttgt
    12721 taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca
    12781 gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg
    12841 cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca
    12901 ttgttcctcc tgaaaataag aatgccacgc aatccgtggc attgttgagc gctattttac
    12961 gtgttttttc gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact
    13021 gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac cacgccattc
    13081 cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt
    13141 atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg
    13201 tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt caatgcgact
    13261 gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcctttca tcacttcaaa
    13321 aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc
    13381 aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt cacccacgcc
    13441 ctgctcgctc actttggtgg acgatccaaa ccagttccac gggttagcgg ccgaccagtt
    13501 aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata acattaaacg
    13561 cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac
    13621 caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag
    13681 cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga
    13741 ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg ccaacagcaa
    13801 cagaaaatag agcacatgcc aagtgcgagg aaatgtagcc cgccgaccgc tcaacaaaaa
    13861 cgccagcacc gccggaatgc ctggaatcag ccccagccag aaattatcgt gatcgggata
    13921 aaacagattt agcagcgcag taccctgctc gcgcgacgca ccggcaatga caaacagcac
    13981 ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgtcc
    14041 gtgcgcatca taatggacag gatggaactc agtactcttc atcttcaatc aaacgcttac
    14101 ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt
    14161 cgttatcttc cggcacattg atctgaattt tcgggcagcc acgagcaatc agctttttct
    14221 ccagccgatt aagcaacgca ttggcaatcc cacgcccacg aaactctgga tgcacgccaa
    14281 gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac
    14341 cgtttacctc agcgaccaaa aacaaactga cgtcatggtt catcttacgc tcgatgtcca
    14401 tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt
    14461 cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta
    14521 aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa
    14581 aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcaacatc
    14641 aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt attacaactc
    14701 aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa
    14761 agccggtttg gctgccctga cgttgccagg aatgtcgcaa gccatcgcca aagacgaacc
    14821 tttaaaaacc agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt
    14881 cgttgttctc gatccaggcc acggcggaat tgataccgga gcgatcggac gcaacggttc
    14941 gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca
    15001 tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt acgatcgcgt
    15061 tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg atggctttac
    15121 caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc
    15181 aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc
    15241 gactgacaag gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat
    15301 taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag attaagccgg tgcataaact
    15361 gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc
    15421 ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt taggcacggc
    15481 ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt atttccactg
    15541 gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg acgcacacca
    15601 ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga ccgccgtcga
    15661 tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg
    15721 ggtgttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttctc acgtccacgg
    15781 cgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga
    15841 ggcgatgggc gtttcactgg tagtgcatcc gcataacccg tatgttccca ccagccacgc
    15901 gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag cccgtctggt ggtttggcgg
    15961 cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact ggcaccgcac
    16021 cgcccgtgac ctgtgcctgc catttggtga agacgtttat ccccgttaca aaaagtggtg
    16081 cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt
    16141 tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa
    16201 aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg cctacggcga
    16261 gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca atctggtctg
    16321 ggatcgcggc actctgtttg gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc
    16381 aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca gcccagaagc
    16441 ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg
    16501 catccgctga tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc
    16561 aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg
    16621 cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc
    16681 gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg
    16741 cagttgatta cacaagtcca gcaccgtcac cagctcagac atattttcca gcacatattc
    16801 acgggcgcgg gaaagtaatc tacggtaact ctgatgactg atgctttccg ccgtcaccat
    16861 tggttgcgcg tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt
    16921 tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg
    16981 gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg actggctacg
    17041 cagcatatgt aatacccgat ccggattgtg caaaaagtta gcctgacggg tgatgacatc
    17101 ttcagacagc actacgccca ggatcgtata atcgtccggc gtacttaatt caaactcggt
    17161 tccaccgggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga taaatccctg
    17221 ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg
    17281 acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagca gaatttccgt
    17341 aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat
    17401 cgtcaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac
    17461 cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc
    17521 tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac
    17581 aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa
    17641 gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg cagccacttc
    17701 tcctgccgtc attccctgac gtactgatgt taacagcgcc aacaactcgt cagcagattc
    17761 cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc ggttaaagcc
    17821 agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttgcggc tgatgacacg
    17881 cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc
    17941 gccttccacc accagcgtta agcgaccagg gtcaagcact tcgtgactga gcagacgaac
    18001 gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc
    18061 cagcaatccc agtgcattga tcatcggcaa cctccgttac gcacgctgga ttggattacg
    18121 cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct
    18181 accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacat aggtcaccag
    18241 ctgtacgtcg gcagatttca acgctgcatc aatgccgtag gtcgcttcca gcggcggtgc
    18301 caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaaa gataagaacc
    18361 ggtacgcgaa actacatgtg ccaggaatgc ggtgttttct gcgtcattag cccactggaa
    18421 agcagcgcca ttttcaatat gcgcaaccat cgcatccaga ccagcacgca cttccgccgg
    18481 gtttggccca ccaagcataa tcagcacttc accggcagtc ggtgacggtc cgtgtgctgc
    18541 tcccgcatac agcgagcggc catataccac ttcgaccatc gcctgcttgg tcgcttcgtc
    18601 agccgcaatg taggtgacgt catcggaatc agcagaaatc agcccgaggc tacgaatatg
    18661 cggcggcaat tttagttcac gcgcaaattc agcgttaacg gaggcaatca cccgcatggc
    18721 ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt
    18781 gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga cggctgcggc
    18841 ttcaaccggc ggcgtgccgc cctggtgaat gttagagatg caggtgcgat cggcctcaac
    18901 ggtggtcgcc atacgcggcg agtaaacggc gtaacaggag aggctttctg actgacccaa
    18961 ccccggacgt tcccccacca gcaggatcac cactttcgcg ccgaggatct caccgatctg
    19021 atcttcaatc ttcacgcgac cataacgaac aaagaatggc gtgccgactt tcagcccggc
    19081 ctgtttcagg cccgccatca gcggcggcag gatctcttcg tagttcacgg tgatggcatc
    19141 agttgacagg ccatcagaaa taacgacctg tacgtccgga ttagcaacac actgtgcttt
    19201 cagcgcttca acagcttctg cacacaggcg gcggcccata tccgggcgag tcaggtagag
    19261 atttttgtcg ctaatttcgg agcgtacttc cagcaagccc tgcgctttca cccactcttc
    19321 cggcacttct ttcagtacgg tatctttcga acgagagtga tcggccagga aacgcagcag
    19381 cgcctgcgta cgtggacgcg gaccggtacg accggtacaa acgcgggcca cggtgctgcg
    19441 gcgcagttct gttaatacgt ctgcgcgatg cgggttttca acaccaatcc acgcttttgc
    19501 ttccgcagaa cctaaatcca gcgcgcagct ttccgaggtc accggtgccg cacagttagt
    19561 ggtggcgcac tttgcttctg acggcgcggg ggccgcttgt cccattgacg ccatcacgct
    19621 gcgtacaatt tcttcaatct gtttttgatc catgatgtgt tatctccgcg tcatcagaag
    19681 aacagtgacg gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat gcccatgctt
    19741 tccagccagc gttcaaactc cggtgacggg cgcaggttga gtaactgacg cacagtggca
    19801 gtgtcgtgga atgcggtggt ctgatagttg agcatgatgt catcgcccag cggcatcccc
    19861 atgatgtagt tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt gaggttctgg
    19921 tcagcgtcag cgtggttggt gtaacagcag tcacagccca tagagatgcc gctcagcttg
    19981 cccataaagt gatcttctaa gccagcacgg ataatctggc ggtcgttgta gagatactcc
    20041 ggcccaataa agccgaccac ggtgttgacg ataaacggat cgtaatgacg cgccagccca
    20101 tagttacgtg cttccatcgt cacctggtct gcgccgaagt tagcgccagc agacagcgcc
    20161 gaaccttgcc cggtttcgaa gtagaggcag ttttccccgg cgatacggtt gaactcagca
    20221 cccactgcgc gcgcttcgtc gagcatcgcc agttccacgc caaactcttt cagccctttt
    20281 tcgctgccac agatactctg gaaaatcagt ccgccaggcg cgccgcgacg aatcgcttcg
    20341 atctgggtgg tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc
    20401 acgccgtaaa tggtgtccaa cacgcggctt aagttttcca cgtcgtcagt taccgggtta
    20461 acgccgatca ccgcatcgcc caccccgaag gaaagccctt cgtagatttg tgcagcgata
    20521 ctttgcacgt cgtcacgggt gtcatttggc tgcaaacggg cgctgaaggt gcccggaata
    20581 ccgatggtgg tattggcctt tttgattacc ggcattttct tcgcgccgta gatcaggtcc
    20641 gcgttggagc aaatcttcgc taccgccgcg accacttccg aggtcagccc tttgcgggta
    20701 aaggcaatgt cgtccacgct ggtttcatcg ctcagcacat actcacgcag ttcgctgatg
    20761 ctcctgtttt taatctggtt gtaggccgtt tcgttaacat cgtcctgaat cagccgcgtc
    20821 acgcagtcat cttcataggc aatcaccgga ttattgcgga tgtccgctac ggtcatttcc
    20881 gacaacacct gctttgccgc cacgcgctcc tgtgagcttg ccgctgcaac gcccgccagc
    20941 acatcccccg aacgcagttc gttggcttta gccagcacct cttttacatc cttaaactga
    21001 tatacattgc cgaacaatgt ggtctttagt ttcataagtc gttccctcag gaaggaaatg
    21061 cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga
    21121 tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt tgttgtagct
    21181 gtgggcgcaa caacatgccc agagctttac caaagtcctg cccggccacc accagcaggg
    21241 gatgcggatt tggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc gtcagtaccg
    21301 cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca gttttgggat
    21361 caagatccag ctgaagcagc gcctgttgcc aggcactcac cagatccgtt tcatcaatcg
    21421 ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctcaagccag attgtgctgc
    21481 cagagagcga aagggtatgc gcgcccgcgc caatcaccgt ggcgcgcacg gtttgcgccg
    21541 gaaactgtac gttcatctca cgcaggcgcg gatggtcatg cagcgccgtt gccagcagcg
    21601 ggccaatatc ggcaaaacag aacgggtcgg cgggctggtg gcgataacat tcgcccacgc
    21661 cgccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca
    21721 atgcctgcgc gagcggcgag agcgttccgt caatcacttc gacaatcagt tctgccatcc
    21781 gccgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcggtg cctgcgccga
    21841 agcactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc ccctggctgt
    21901 cggtttccag caggcgacca ccgacgttga ggcaggcagt gccgctgatt tttccggcat
    21961 cgaacagggc gtagttcgca gtgccaccgc cgatgtcaat attcagtacc cgacacagcc
    22021 gttgttcaga aagggtttgt gccccggcac cgtgaccggc aatgacggat tcgaggtgcg
    22081 gcccggcgct ggcaacgaca aaatcgccca gcgactggga gagcaccatc accgccggac
    22141 gagcattgcg ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt
    22201 ccggcgcaat gcccgcagcc tgatattgct cgaggattaa ggttttcagt tccgcttctt
    22261 ttaaaccacc ctgtttatcg acaggggtaa agaacaccgg gctttgccag ctaatttcgc
    22321 gtttaatgaa ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc agctccaggt
    22381 gggagaaaat cacctgggtg gtggtggtgc cgatatcgat accgacgctc aatagctggc
    22441 gagtgttcac gattgtgcct ccgcttcggt tttagccgtg gtcgcgtctt cttttggcac
    22501 cagcatcatc gccacgccaa tcgccgttac gccgccgatc aacttgccga caatcatcgg
    22561 gaagatcatg gcgttcatgt tggcagcggc gaagcctaag tggtcgccca gggcgaaagc
    22621 agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg gtatccatct gcttcatcat
    22681 gccgaacatc gggatgttgt tggcaagcgt tgccaccatg ccggctgccg cgatgttgtt
    22741 catattcagc actttaccga cgctcatcag cggtttttca aaccagcggg tcagcagcag
    22801 caccattgga tacgccccta acagaacgca ggagatagaa ccgataactt caatggcgcg
    22861 catcacttca ccgggtttat cgccaggggc cataaagata gggtccagac cggggatcag
    22921 ttcccagcca agcagaaact taactaccgc ggcagcaaga ccgagggtga tcaatgcaac
    22981 gaggaatttg gcgaagatct ggaagccgtt gatcattttt tccgggatga atttcagccc
    23041 cagcgccacc agaatcgcaa caatgatcac cgggatcatg ttcatcagga tcagggcgaa
    23101 agtgaattcc accggctgac cgttgatctg cacaccggag tacatagcaa ccagaccacc
    23161 agcaatacag ccaatcggaa tggtcacaat gcccgccagc acgccgagcg ccagataacg
    23221 acggtcagaa ggttcgataa tgccgagcgc caccggaatg gaaaacacaa tcgttggccc
    23281 catcatcgac ccaagaatta acccagagta tagccacgct gctacgtcgc cgcccgccag
    23341 ctctttggcg aggaagaagc cgcccatatc gcacgccagc agcgttccgg cgaacattga
    23401 cgggtttgcg ccgagcattt cgtaaaccgg gataattacc ggcccgagta cgtgagccag
    23461 taccggtgcc agcgcggtca taccgaccat cgccagaccc agagcgccca ttgccataaa
    23521 gccttcttcg aactgaccgc ctgatccttc gatactttta ccgaacttac cgaggaaacg
    23581 agcagaaccg ccgaactgcg acaggatcct gtctacggca gctatcagca taaagaacat
    23641 catgatgtac atgatgattt cgttaattcc catcgccttt actccctgtt agttgttatt
    23701 tattggcgga tgcggcgtaa acgccttatc cgccctacat gtgcaatccc gaaggccgga
    23761 tcagacgcgg caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgtga
    23821 acgccttatc cgccctacat gtgcaatccc gtaggccgga taagacgcgg caagcatcgc
    23881 atccggcaat tgcaccgcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    23941 gtgcaatccc gtaggctgga taagacgcgg caagcgtcgc atccggcatt tattgcgccg
    24001 ccgcgtacag tccgacaatc tgttccaggc tggcggtacg cgggttactg cgcagacaaa
    24061 tatcttccag cgcggcctgc gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc
    24121 ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta atagcgtcac
    24181 gatcgtcgga ttttttagtt cgcagtgccc gaccaatctg actaaagcgt tcacgacaaa
    24241 ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga ccgtgtggaa
    24301 tatgcagcgc cgcgcccggc tgatgcgcca tcgcgtggca caacccaaga cccgcactgg
    24361 aaaacgccat tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcgt
    24421 gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca
    24481 gactgtcggt aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg
    24541 catcaatgcc ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaac gcggcgtcga
    24601 gaatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg
    24661 cgtcgataat caccgttaca ttggtggttt cagagccggt tccggcggtc gttggaatgg
    24721 caatcagcgg caagcgcggt tgcagaacgc tggtttctga catctctgcc agcgtgctat
    24781 ccgggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag ccgccgccaa
    24841 atgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca cacacgtcgg
    24901 tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatgcct ttaacagcca
    24961 ggctgcgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct gccatcacga
    25021 acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc gagcccggac
    25081 cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc agggtatcga
    25141 acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc tttctctcgc
    25201 ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt
    25261 aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca agagccgccc
    25321 gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat gtccgccatt
    25381 ttttcgtaca ccggtttcac cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc
    25441 tccgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat gtgatgtccg
    25501 ccggttgctt catccgccga gtacgtcacc ttgccctttt tcacgatggc aatgccggta
    25561 gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg
    25621 gttggctcat ccagcacatg gctcacttcc agtccagcag actccagcac gttgatggaa
    25681 atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg gcgaccgaat
    25741 tgctgctcga gcgtgtcgag atggcgacga acaatggtga cagcgccgaa gaaatcccag
    25801 acgatgccgt cgcggaccac atcggcccag tcgaggcata ccgccaccga ctgaccgtcg
    25861 cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag ccacagcgga
    25921 gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg
    25981 agccattgtt cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa
    26041 tacacagcga cgcagacgga caaacgtacg cacgctagtt accccttcac cggttggcgt
    26101 ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa tgcgcggtcc
    26161 gttcttaacg aaaatgctgg tatcaatagc attcgccatc tggttcatgt tttcgatgtt
    26221 gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg ccagcgcaat
    26281 ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca gttcggtcac
    26341 ggcaaacgga tgttctgcag tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt
    26401 caggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac ggctgacggt
    26461 gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct gcgcctgttc
    26521 tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat cggctacgct
    26581 atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc
    26641 tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca ctaccggcgg
    26701 gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg cttctactac
    26761 cgcttcgccg ccggtcacca ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc
    26821 ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccgc ctgcggcaac
    26881 aatcgcctgg ttgagcagcg tgatcgcccg ctgggagact tttttcgccg ccggatgcgg
    26941 ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttattaa ttacggttgc
    27001 cgccgggtta gtggacggag ttaccgaagc caccacgccc caaggcgcgt tttcaattaa
    27061 agtcaggccg ttgtcgccgg tcagcacctg cggagagaga cactcaacgc ctggcgtgcc
    27121 gcgcgcctga gcgacgtttt tggcaaattt atcttcaacg cggcccatgc cggtttcact
    27181 gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat
    27241 ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg ctgccgcaac
    27301 ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact
    27361 gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat
    27421 gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc gacaatgcca
    27481 atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca
    27541 ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcgcat
    27601 tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt gtcatgcgcc
    27661 agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc
    27721 gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa
    27781 aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc
    27841 gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg ggataacgtg
    27901 tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt
    27961 acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac
    28021 accaaccagc ttgacgcgcg cagctttaac catcgcgtca gaagcctcaa tcagtgcaac
    28081 caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc
    28141 cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc
    28201 tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct acaaccacga
    28261 gagagatcgt gcatcggcgc ggcaagtcct tgtatcaatg gcccgacggc acgatatccg
    28321 ccgagtcgtt gtgcgatttt gtagccaata tttccggctt ccagcgacgg aaaaaccatc
    28381 acattggctt tgccctgtag cgggctggca ggcgcttttt gcgccgccac ttccggcacg
    28441 aaggcggcgt caaactgcaa ctcgccatca accaccagct ttggtgcgcg ctcacggacg
    28501 atttctgtcg cctgctggac gttggcaacg caggggtgac gggcgctacc gttactggaa
    28561 aacgacagca tcgccacgcg cggctcttct ccggtgaggg cgcgccaggt ttcggcactg
    28621 gcaagcgcga tatccgccag ttgcgccgcc gtcggctgtg gcaccacgct gcaatcggca
    28681 aagcccaacg ccggaccgct gtactgtggc agcatcagga aaatggatga gagcgtttta
    28741 cagcctggct gcaagccgat aatacgtaat ccggcacgca gcacattcgc cgtggaagag
    28801 aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc ggcgaacatc
    28861 agcggatcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc
    28921 cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat cacctgtagc
    28981 ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attagcgacc
    29041 agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cagctttcag cacacgttga
    29101 tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc acgacaacgt
    29161 tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcggcggct
    29221 tcgttacgct caggatcatc attacgtaga ccgtgctcga aaggcggttc agcgcctgca
    29281 agatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc gctacggttt
    29341 cggtttcccg cactttggta cgtagcagat tgagcaacgc cgcatcgcgc ccgtggctgg
    29401 cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctggtgagaa agtcgatgca
    29461 gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttccccc agcgcatcgg
    29521 cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc cacggttccg
    29581 ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc gcgcgaaaac
    29641 ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc agcgtatccg
    29701 gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt tcatcgctac
    29761 tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac aggcggccct
    29821 gctcgtcaat aaacttgatg cgcagatggc ggctttccag caactcccgg gcagagggcg
    29881 tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta tggttcgctc
    29941 ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg gccaggcgac
    30001 atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt tcgggataaa
    30061 catcacgtcg cctgctttgg cgatcatggt ttcgccttcg tggcgaacat ggagctcccc
    30121 ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg cgttctccca
    30181 ctgcataaaa ccggcggcca tgctgctgcc gtcgtctccg gtcacaagat cagtcaagcc
    30241 cacgcaatgc ggttgtgcgc catcaaaacg accaaacttg acgctgctgc catcaatcac
    30301 tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc cctgctccag
    30361 cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt cggtaaactg
    30421 gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc
    30481 tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt cggtgatggt
    30541 aaaacccagt agatccgcca ctttacgggc ttccggggta ataatgctgg cgcgcagaac
    30601 cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    30661 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    30721 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc gtctcaagca
    30781 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    30841 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    30901 ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt aaggcgtgat
    30961 accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta tcattaaatt
    31021 ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    31081 tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc tcgtattaac
    31141 ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg acggtttgtg
    31201 ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg ctgaagcggt
    31261 cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccgacg atcatcgccg
    31321 tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    31381 tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca
    31441 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    31501 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    31561 ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca tatgcaacgc
    31621 atgaatataa ataaaataat atcgagatat tatatagact gctttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataactta gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacgtc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt attgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattca ggttaaaata
    46741 cagacagata acaagatgaa tattcttaat gtttacgtta aaaat
//
LOCUS       DAZPLM010000008        46166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPLM010000008 DAZPLM010000000
VERSION     DAZPLM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.008"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHVC7_000378"
     CDS             complement(<1..716)
                     /locus_tag="ACHVC7_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2114526.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHVC7_000379"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHVC7_000379"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR2114527.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHVC7_000380"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHVC7_000380"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR2114528.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHVC7_000381"
     CDS             complement(3650..4705)
                     /locus_tag="ACHVC7_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2114529.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHVC7_000382"
     CDS             complement(4715..5503)
                     /locus_tag="ACHVC7_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR2114530.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHVC7_000383"
     CDS             complement(5521..6573)
                     /locus_tag="ACHVC7_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR2114531.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHVC7_000384"
     CDS             complement(6604..7446)
                     /locus_tag="ACHVC7_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR2114532.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHVC7_000385"
     CDS             complement(7433..8314)
                     /locus_tag="ACHVC7_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR2114533.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHVC7_000386"
     CDS             complement(8335..9645)
                     /locus_tag="ACHVC7_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2114534.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHVC7_000387"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHVC7_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHVC7_000388"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHVC7_000388"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR2114535.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHVC7_000389"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHVC7_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR2114536.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHVC7_000390"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHVC7_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR2114537.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHVC7_000391"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHVC7_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR2114538.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHVC7_000392"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHVC7_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR2114539.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHVC7_000393"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHVC7_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR2114540.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHVC7_000394"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHVC7_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHVC7_000395"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHVC7_000395"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR2114541.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGCTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHVC7_000396"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHVC7_000396"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR2114542.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHVC7_000397"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHVC7_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR2114543.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHVC7_000398"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHVC7_000398"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR2114544.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHVC7_000399"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHVC7_000399"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR2114545.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHVC7_000400"
     CDS             21883..22047
                     /locus_tag="ACHVC7_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114546.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHVC7_000401"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHVC7_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR2114547.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHVC7_000402"
     CDS             23556..23801
                     /locus_tag="ACHVC7_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR2114548.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHVC7_000403"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHVC7_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR2114549.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHVC7_000404"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHVC7_000404"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR2114550.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHVC7_000405"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHVC7_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR2114551.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHVC7_000406"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHVC7_000406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR2114552.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHVC7_000407"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHVC7_000407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR2114553.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHVC7_000408"
     CDS             29464..29817
                     /locus_tag="ACHVC7_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR2114554.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHVC7_000409"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHVC7_000409"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR2114555.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHVC7_000410"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHVC7_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHVC7_000411"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHVC7_000411"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR2114556.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHVC7_000412"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHVC7_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHVC7_000413"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHVC7_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHVC7_000414"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHVC7_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR2114557.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHVC7_000415"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHVC7_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR2114558.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHVC7_000416"
     CDS             36881..37630
                     /locus_tag="ACHVC7_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR2114559.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHVC7_000417"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHVC7_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR2114560.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHVC7_000418"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHVC7_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR2114561.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHVC7_000419"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHVC7_000419"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR2114562.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHVC7_000420"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHVC7_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR2114563.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHVC7_000421"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHVC7_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR2114564.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHVC7_000422"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHVC7_000422"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR2114565.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHVC7_000423"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHVC7_000423"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR2114566.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHVC7_000424"
     CDS             complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHVC7_000424"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR2114567.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHVC7_000425"
     CDS             complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHVC7_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR2114568.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHVC7_000426"
     CDS             complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHVC7_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR2114569.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45938..46078)
                     /locus_tag="ACHVC7_000427"
     CDS             complement(45938..46078)
                     /locus_tag="ACHVC7_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR2114570.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        10928 a        12206 c        11623 g        11409 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cacccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcagcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44701 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44761 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44821 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44881 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44941 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    45001 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    45061 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    45121 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    45181 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45241 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45301 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45361 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45421 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45481 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45541 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45601 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45661 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45721 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45781 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45841 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45901 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45961 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    46021 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    46081 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    46141 caaatcctaa agaaacttta gtcaaa
//
LOCUS       DAZPLM010000009        46084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPLM010000009 DAZPLM010000000
VERSION     DAZPLM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.009"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHVC7_000428"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHVC7_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR2114571.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHVC7_000429"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHVC7_000429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR2114572.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHVC7_000430"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHVC7_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR2114573.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHVC7_000431"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHVC7_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR2114574.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHVC7_000432"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHVC7_000432"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR2114575.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHVC7_000433"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHVC7_000433"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR2114576.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHVC7_000434"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHVC7_000434"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR2114577.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHVC7_000435"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHVC7_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR2114578.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHVC7_000436"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHVC7_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR2114579.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHVC7_000437"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHVC7_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR2114580.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHVC7_000438"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHVC7_000438"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR2114581.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDCTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHVC7_000439"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHVC7_000439"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR2114582.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHVC7_000440"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHVC7_000440"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR2114583.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHVC7_000441"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHVC7_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR2114584.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHVC7_000442"
     CDS             complement(14282..14581)
                     /locus_tag="ACHVC7_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR2114585.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHVC7_000443"
     CDS             complement(14793..15233)
                     /locus_tag="ACHVC7_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2114586.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHVC7_000444"
     CDS             15334..15933
                     /locus_tag="ACHVC7_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR2114587.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHVC7_000445"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHVC7_000445"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR2114588.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHVC7_000446"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHVC7_000446"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR2114589.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHVC7_000447"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHVC7_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR2114590.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHVC7_000448"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHVC7_000448"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR2114591.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHVC7_000449"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHVC7_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR2114592.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHVC7_000450"
     CDS             complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHVC7_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR2114593.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21614..22777)
                     /locus_tag="ACHVC7_000451"
     CDS             complement(21614..22777)
                     /locus_tag="ACHVC7_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR2114594.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22777..23256)
                     /locus_tag="ACHVC7_000452"
     CDS             complement(22777..23256)
                     /locus_tag="ACHVC7_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2114595.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23271..25718)
                     /locus_tag="ACHVC7_000453"
     CDS             complement(23271..25718)
                     /locus_tag="ACHVC7_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2114596.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25711..25866)
                     /locus_tag="ACHVC7_000454"
     CDS             complement(25711..25866)
                     /locus_tag="ACHVC7_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR2114597.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25863..26138)
                     /locus_tag="ACHVC7_000455"
     CDS             complement(25863..26138)
                     /locus_tag="ACHVC7_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR2114598.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26195..26713)
                     /locus_tag="ACHVC7_000456"
     CDS             complement(26195..26713)
                     /locus_tag="ACHVC7_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR2114599.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26726..27916)
                     /locus_tag="ACHVC7_000457"
     CDS             complement(26726..27916)
                     /locus_tag="ACHVC7_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR2114600.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28327..29745
                     /locus_tag="ACHVC7_000458"
     CDS             28327..29745
                     /locus_tag="ACHVC7_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114601.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29860..30387)
                     /locus_tag="ACHVC7_000459"
     CDS             complement(29860..30387)
                     /locus_tag="ACHVC7_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2114602.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30391..32709)
                     /locus_tag="ACHVC7_000460"
     CDS             complement(30391..32709)
                     /locus_tag="ACHVC7_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR2114603.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32720..33250)
                     /locus_tag="ACHVC7_000461"
     CDS             complement(32720..33250)
                     /locus_tag="ACHVC7_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR2114604.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33243..34151)
                     /locus_tag="ACHVC7_000462"
     CDS             complement(33243..34151)
                     /locus_tag="ACHVC7_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR2114605.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34156..34503)
                     /locus_tag="ACHVC7_000463"
     CDS             complement(34156..34503)
                     /locus_tag="ACHVC7_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR2114606.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34500..35135)
                     /locus_tag="ACHVC7_000464"
     CDS             complement(34500..35135)
                     /locus_tag="ACHVC7_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR2114607.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35202..35654)
                     /locus_tag="ACHVC7_000465"
     CDS             complement(35202..35654)
                     /locus_tag="ACHVC7_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR2114608.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35647..36114)
                     /locus_tag="ACHVC7_000466"
     CDS             complement(35647..36114)
                     /locus_tag="ACHVC7_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2114609.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHVC7_000467"
     CDS             complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHVC7_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR2114610.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHVC7_000468"
     CDS             complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHVC7_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR2114611.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36635..37060)
                     /locus_tag="ACHVC7_000469"
     CDS             complement(36635..37060)
                     /locus_tag="ACHVC7_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR2114612.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37075..37572)
                     /locus_tag="ACHVC7_000470"
     CDS             complement(37075..37572)
                     /locus_tag="ACHVC7_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR2114613.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37572..37853)
                     /locus_tag="ACHVC7_000471"
     CDS             complement(37572..37853)
                     /locus_tag="ACHVC7_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR2114614.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37857..38060)
                     /locus_tag="ACHVC7_000472"
     CDS             complement(37857..38060)
                     /locus_tag="ACHVC7_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR2114615.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38060..38569)
                     /locus_tag="ACHVC7_000473"
     CDS             complement(38060..38569)
                     /locus_tag="ACHVC7_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR2114616.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38669..39412)
                     /locus_tag="ACHVC7_000474"
     CDS             complement(38669..39412)
                     /locus_tag="ACHVC7_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR2114617.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39416..40488)
                     /locus_tag="ACHVC7_000475"
                     /pseudo
     CDS             complement(39416..40488)
                     /locus_tag="ACHVC7_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40547..41401)
                     /locus_tag="ACHVC7_000476"
     CDS             complement(40547..41401)
                     /locus_tag="ACHVC7_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR2114618.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41575..43347
                     /locus_tag="ACHVC7_000477"
     CDS             41575..43347
                     /locus_tag="ACHVC7_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR2114619.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43347..44381
                     /locus_tag="ACHVC7_000478"
     CDS             43347..44381
                     /locus_tag="ACHVC7_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2114620.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44753..45895
                     /locus_tag="ACHVC7_000479"
     CDS             44753..45895
                     /locus_tag="ACHVC7_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR2114621.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45897..>46084
                     /locus_tag="ACHVC7_000480"
     CDS             45897..>46084
                     /locus_tag="ACHVC7_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114622.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNL"
BASE COUNT        10852 a        12390 c        11826 g        11016 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattgca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tcgaccataa ataagcccct caactgaggg
    21301 gctatttttt tgatcacatc cacataattt gctgccctga cggcaacggg tgcggcctta
    21361 cggcgtggac ttctcccggc ttcacgatgt atcgctgtac cgactcataa gtgatgaacg
    21421 tggcgctgca attcacgttc tggcactggt gataacgctc ttttgtcgtg tcagtgatat
    21481 agcgacttgt acgcgcatgt gcggcatgct ggcataaagg acaatgaaac atcgcgagca
    21541 cctcttccgg ttttgttgat ggtgccattt tagttaattt atccttataa aacaaacaga
    21601 taaataaacc acgtcactca tcatcttctg tttcgtactc cacatcagaa agccttacct
    21661 caagctccaa ggacgtcgtg aagccgctat tattgagaca atgtgtcacc ttagtgatgg
    21721 tccagtcctg ctcgtctatg acgcgcttaa agcctgacac tttaaccggt gtttccgtgt
    21781 aaatatctgc acgaccggta gccaggctga tggagaactc cgctacaccc cgttgcagtt
    21841 tatcccactt cgcctgagcg gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca
    21901 gggcaaaaac gttgtcagct tcaccggcca tgtattcacc ttcgcgcgct tccggtactt
    21961 ttggcgcttt cttctgcgtg accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt
    22021 tttctttctt tttgcgtttc agttttacct tctgcttttg cggcttcggg tctttggtgt
    22081 gtaaccactt tgccgttacg ccggtgtaag ctccacggtc agcaatcgca aaatgatgac
    22141 ggtcgccgtc gctgcgggtg atggtaatct gcgggatttt tttaccgctg gccgtcaccc
    22201 cctgccccgc tttgagaaac agcagttttc ccatttttac cgacacctcg ccgccgttgc
    22261 gttcagcaag acgggtcagg aatttcgcat cagactcctg cgactggtcg atgtgtggaa
    22321 tttttattcc ggccagtgac ggagcgacac tggcttccag cctgttacgg gaggctatcg
    22381 cctcaacaat cgcaccgagc gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg
    22441 tcccgcgaaa atctgcactc cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt
    22501 caacctcatc aacggtaaat ttccctttgc ataccagggc aaaacctttc cagccgatat
    22561 acaccgtcag gacagcgcca cgaaccggca gcccgacctg cccgtcggca tcgttcagtt
    22621 caatatcaag ctggtcagcc tcaaagcccc ggttatccgt cagggtcatg ctcatcagac
    22681 ggtcgctgat attgccggta atatccctgc tgtcgagcat cagcatgtaa tccggcgtca
    22741 gcgtactgcc tgcatcaaat gtcagcgcat ccagcattat cccgcccccg tcatacccgt
    22801 gaatttagtc gccatactgc cagccttacc gatgagcgat tccgcctgtt taccgatatc
    22861 gccataaagc gcggccagtg actcatcaac gcgggtgagc gacagcgtaa aatcaatttt
    22921 ccgtggtgtg ccgtctgcaa agaaaatact ccctgtttca ctcaccttgc tgatgacata
    22981 catgccgtaa atcatgccgg tgccatccag caacggccac gcccggcctt cctctgccat
    23041 cagtctgagc gtggtcatcg tcagctttcc gccagtcagt tcgggataaa gcacaccggc
    23101 aagtgtgatg ttctcctcac ctacaccgag aaactgatag gcatcccgtt taccgatacg
    23161 ggaatttgac ggccagcgat aatctgattc acgctgcatg gtctggtgtg gcagcgtctg
    23221 gcgcataaaa acaaacatac ctaacgcgag catcattttt cgtcacctcc ttaaccgtca
    23281 tgcatcatgc tggcacgggc gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc
    23341 tgtaactggc ggtcaagctg tgtccccggt gcagtaccac ccgtcaggct gatgtgatat
    23401 tcgtttttac tctggtccac ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc
    23461 tgatagcctg cataagagct ggtcgccgga atataaccac cggtgccata cgtggcggca
    23521 tgagttctgg cggcggtctg gtcaagtgtg tctgactctt tgttgatgac cccgagcttt
    23581 tccagtaccc agtcaatgcc gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc
    23641 gcgtcagcca gtgcctgccc gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc
    23701 tgggtggctt tgaccggggc aatcaggttt ttaaaccact gccacgcagc ctgtaacttt
    23761 tcgcccagcc agtcaaacac cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat
    23821 gccgctttca gcccttcaac cacaccgcca aagaatgcgc tgacaggctc ccagtattta
    23881 cggataagca acgccccggc gacaatggcg gccaccacag ccacaaccgg ccagctaatc
    23941 gccccgatgg ccgtcataac ggcactacca accgtcgtga agattgcccc cattgcgcct
    24001 gctgccgcga tgatggcatt gatgccggtg ataaccggcc aggctacaag accaattgca
    24061 ccgatgatgc cagtcagcgc cagcgcgcca ccggcaatga ggccaatggt tgacgccagt
    24121 gatttgtttt tctgtaccca gccgtcgagt tttaacacat actttgtggc cgtctgcgtg
    24181 agcttacgta gcgcgccttc ctgctggtca aacaggtctg tccccaccgc ctcataagcg
    24241 gactgaaact ccttaaagtc accgccgagg ttgtcctgca tgatatttac cagctctgcg
    24301 gtcttcccgt ctgaggcttt aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg
    24361 gcagtcatca gcacagcggc ggctgagctg gcctcctccc cgaaaatggt tttcatgtat
    24421 tcggcctgct gggcagtacc gagccggttt ttctcaaagc tggcctgcat ttctttcaga
    24481 atggtaaata ctggtcgggt gtttccctta ctgtctgagg ttttcacgcc aagctctttg
    24541 agtgcatccc atgcttttcc agtcggtgcc tgcaggcgac ttaacacggc acggcttccc
    24601 gtccccgcca ttgagcctgt gatttttgca tcatgcagcg ccccgaccat tgcggcggtt
    24661 tcttcaatgc tgacaccggc attttttgcc actggtgcgg cataggtcag cgcatcgctc
    24721 atgccgtcaa aatcagcggc ggttttgttc atcgtcatgg agagaacatc cccgatatga
    24781 gcgaccttat cgtttgaaag ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt
    24841 tcttccattg tgcggcggtt cgccagcgcc atattcagcg tgaccggcgt tgccgcctga
    24901 atggcatcaa catccccacc cgctttcgca atgataatct gtgcaccggc cgcatcatcc
    24961 gctgaggcgg cggtgttgtc accgagctgg cgcgcctgct tgcggagtgc agccatttcg
    25021 gcggagtctt ttgccacacc gagcacagcc tgcaattctg agtttttctg cgcaaactca
    25081 taacctggca tcagcagctt aactccggct atcgttcccg cagcagcaat ccccacaccg
    25141 gcagcgccca ctgaggccat atttccggcc agttcctttc cggcctgata acgctgtttg
    25201 actgcgttaa gttttgcctg ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta
    25261 agctgggcgg tggtttcact gatacggttt ttcagtccct gctcatcatg tgcaagattg
    25321 cgggtattaa ttcccacagc ggccagttcc cgctgctggc gtttaacgga atctgtcagg
    25381 cggttatatt tcgcctgtaa gtcctccgcc gcacgctttg cggattccag cactttcgcc
    25441 tgagcacggg tcgggcgttc ggtgttttta aactgtgtgg caagggcttc ggcctcctgc
    25501 cgtgcctttt caagtgcatg accagtcacg gcgagctgtg cactggtctt gcggaatccc
    25561 tcaatacggg atgcgtgacc gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc
    25621 cccgacagca acttactcgc tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca
    25681 gccctgagca atacctgtaa ttttacattg ttactcattc gtgtttccgc ttcgcctgag
    25741 cgccttttcg cgccatgtga tgagttcggt caggctcatg ggatacagtt ctgatggcgg
    25801 ccagtgaaat atcactgcca catccgccat caggtcatcg accgacagat ttttcggaag
    25861 cgtcactgca ccgagttcgg cgacaaaaaa ccgaccacct taccggccag cgccacaagg
    25921 tcaggcagtt ccagcgcggc gacttcctgc tcggtcagca tcggtgccgt catgcgcggc
    25981 agcaccttaa tcagtgcatc gacttctgag tttgcgaccg cagccagact gacaccgcgc
    26041 agcgtccccg cactgggttt catcagcgtg acctgttcga taacctgctc accacgcttg
    26101 accggattgt ccagggtaat cacattttct ttgttcatgg ttttctcact tctgaatcgg
    26161 ggttaaccgg tcagccaggc tgaccggatg aaaattacag gccgatattg cggcggtgtt
    26221 gctccagccg gtcgacgccg ttcaccttct caatcatgtt gatggtgtcg atttcgacca
    26281 gctccttacc gtccatcgtc agccggaaat aggtgcagac cacggagatt ttcgactcgg
    26341 tgtcttctcc ctgtttaccc tcgccggtgt cgatttcttt ctgacggcca cgcatgacca
    26401 cctctacggc caccgtttcg ccggtatcgt cacgctggta agagcctgca aaacgaatcg
    26461 gcacggcatc cacaccggtt gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc
    26521 cgagcgacca ctccattgac agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc
    26581 cgttcatccc cgcaccgcga tagttttcga gtttacgggt cagttttggc agcgtgacgg
    26641 actttgcaac gccctgatag ctgtagccgt tcagaaagac gttcattaac ttgagtttgc
    26701 gcggcattgc catcggtcag gctccttaat tgctgttaac cgaggtgacc agatttgcca
    26761 ggtatttatc ggtaatacgc tggcgcaggg tcaggttttc aagaggaggc accggggtat
    26821 agtcgtagtc gatatacagt tttccggcct tgagggtttc cgcatcgttg gattcttcgc
    26881 tgaaccagca ggtcgcatcc acgatatagc cgtttgtttt cagctcacgg aatttggcat
    26941 tgatgccgtc aacgatgtcg cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca
    27001 tgtgcgcctc agccatcgtg tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga
    27061 ggaacagcgg gtcatcagag caggtacggt taccccagaa gcggaaaccg tcacggcgaa
    27121 tcagcgttgt gacgcctgac tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat
    27181 cccagaatac ggacgcgctg atgccggtaa caccgttcac cccgacgttg gacagcgttt
    27241 tatgccagcc ctgctcctgg tcgattttag cgcgcagacc cagcgcacgg gcggtggcat
    27301 acgcggtggc ggtggtactg gcgaccgtat cccatgcgag gaaatccggc cagatgacca
    27361 tcagctcacg ctggctgaaa ttctgacggt aggctttcac ctcggaaacg gtcttacagc
    27421 cccatgcgct gatatacccg aaagcgcgca gcttctgaca gactgatgcc agtgcaacag
    27481 ccacctcttt ggtgtccagt cccggcacgc cgagaatacg cggtttaaca ccggttaccg
    27541 actccgccgc cagcagggct ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga
    27601 tgatattgga aacggtctgc gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca
    27661 cacgcacgac aacggtgacc ggttttgact ggtcggcgat ggcctgcaac gatgccgcca
    27721 gcgtgccttt tttaccggcc tttgcaattg cgctctgcac attggtaatc agcaccggtt
    27781 tattgagggg gaaggtttcc gcatccgcat cgctggccgt gcagaccatg ccgacaatgg
    27841 cagtggatac ggtggaaatg acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt
    27901 gatgatagtc actcatccgt ttaactccgt ggttaatggg tgcaactatt ttctgttggg
    27961 cagtgcatga gacgctattt gacctggctg gtcagtggat gaaacaacag ataaagaaaa
    28021 tgcgggcaat tcgcccgcat gtcctgattt gtacacactc attttccgac tgacagttta
    28081 catagcccaa acgctatcaa atctgacagc ctgctatgag cgaaaagcgt taaatctcac
    28141 gatatctgct accgggaaaa atttcgatta tcttcgatgg gaactttgat aatgtagtaa
    28201 atgccatttt cagcgatcac ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac
    28261 ttcactgaaa taaggttgct agtatttttg gagcaaccat taatgttttt tatttaagga
    28321 atgtaaatga catacgcact gattgataat tcaactctaa ctgcagtcca aagaatcgag
    28381 gggaaagtaa aaacaagatc gaaggactca gtagatacag acataatagc atttgagaac
    28441 tatatacagt ctattttgtt ctatgatcac gttatcgcgg ttgatgatta tattccagag
    28501 taccgtggga gtaggatttc aagtttcaga gagatattat ttcttgataa agcacaatat
    28561 gggttggatg aaattgaaag tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt
    28621 cgtggtggaa catttgttaa tgacgatttt aaaaaactaa ttgaacttct tcagacacat
    28681 atcatatgta catgggacat tagttcaagt gtttatcatc taacactgaa gaacttatct
    28741 gatggaggta aggagtttga aaaatatggt aacattgctg ctgccatttt ttcagaactg
    28801 cgtgatgctg ctgagtctgg taatagaaca aatggcgatg ttgaacttct cgatcgattt
    28861 ggcaacccta ttcgcaaggg gtacaaagtt cccggagcaa aatgggggga tggcacatct
    28921 gctggtgaag catcagatgc aatcaaagca ttcgtggctt cgcttgtatg gctggctaat
    28981 cgggcagtat tttattctct cacagctaag taccttcaag cagatacttt tttatatcca
    29041 ttacgtcagg cgtatcaaca gacatattta agccaaaact gtcagtatgg ttttgactat
    29101 gctaaacata ttgtagaaaa cttttccact tcgttaagtc atgatctcat tgatattcat
    29161 tctggtggta aagctgtagc aacagcaact tcgcttcctt tattttcggc atggctcgca
    29221 aaagaaactg gtgatccaag ttgtattgtt gaagctgctt ataacataag ggacaacaac
    29281 gaatttgtaa gggcccgtga gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa
    29341 ttgcctcaag ctaataagtc agtaagagca ttgcttactg aagtatcgaa agaatcagat
    29401 tatattcgaa ctaagtatgg gctcaagact agacaaggtg tacctgtaac taagcttgta
    29461 catgtataca atactgttgc ggctattaat tcccttccga aactaccgac ctttaatttt
    29521 aaagttaaag ttcctgattt cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc
    29581 tatcgaaatt tgactaatga tctttctact gtgtggtcgt taggagaagc tcgagatatt
    29641 cttggaagta aagttgtgat agataatcat gcaagaattt acagtccgaa gcatgaagaa
    29701 ccgcgtttca aaaactctca ttccccatat aagagcccga tgtagcttaa gaaaaagaca
    29761 cgcccacctt gtgcgtctac ttctggcaca gagcggactc tcagattagg ctttactctg
    29821 tgccatagat atgtaagctc acaccagagc tcatacaact tattgcggca tttccggcca
    29881 ttcaggattt gcaggatcca cacgactgac cagaacactg tagcgttccc atgcctccag
    29941 tcgactgcgc tcctcatctg ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat
    30001 cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct gctgcaattc
    30061 ctctgccgta taaattcgtt taatcactgt acagtcctta aacatccagt tccctgaaat
    30121 gtccgcccgt cggttagcag taatatccgg cacttcaaca acacttaatc catccggtct
    30181 gatagctgtc acatcctttt ccacatagcg gataatatta tctttgtcgt acgctatttt
    30241 tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat cttctgaaaa
    30301 aaaccagaca acatcaaagt cctttgtcaa ttgatattgt tcaaccgttt ttggattacc
    30361 tgccgttata tttatcaaat gctgcataaa ttatacctgt gccacgttat accaagtccc
    30421 gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg cagagtttga
    30481 gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct gatgtgtcca
    30541 tgtcatacca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgga tatcccggac
    30601 atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa cagcaaaagt
    30661 gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat cacctaattc
    30721 agccaccaca ggacgacttg agtttcccca caaattgaat gcggcttcct tcgtggatgt
    30781 attgctgctg ctgaccgtga actttttccc gctaccggca cgtactttgg tacttgagac
    30841 aatatcacct gtaacactca ggccatgccc cattgacact ccgccattgg cgttattgat
    30901 agtcagcggt cttaagccgt tccatgtccc aaatttatcg ccagaggccg tcagcattaa
    30961 atatgtgctg ccgccatcat tcctgataaa gaatccataa ttgccataag caatgcgcag
    31021 accattagca ctgagtgatg taatttcacc tcttgaacgg agaccataag cggagctgag
    31081 tgataattct tcctgagcgt cattgtttcc tgtcgcccag cgaattaccc cgccctgaac
    31141 tgtttcatgc cagatagtat ctccttctcc accacgaaac tttctgagat attttttgcc
    31201 gcctctggtg cctgaacata aggccgtaga catataggca ttctggcttc cgccatcctg
    31261 attaatcgtt ccggtcattg agtcgccctg acgattccag tcacgacgcc agccggggga
    31321 gtagccgtcc ccatgattta tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga
    31381 tgttgttggc gtggtcactc ggatagtgat tgcagattta gttcccatga cctcgacgac
    31441 acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt tatttgcata
    31501 tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg aatccagcca
    31561 ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc
    31621 acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg caccgttctg
    31681 atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt ttggcgttgc
    31741 ggccagcgtt tcagaatcgc tgttgatggc actacttagc tggacaagac cttttcgcgc
    31801 tgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg tcttaaccgc
    31861 ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg gcgctactga gctggacaag
    31921 accttttcgc gctgtagtgg cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc
    31981 tgtcttaacc gcctttggcg ttgccgcaag cgtttcagac gtgctgttgg tggcactact
    32041 gagctggaca agaccttttc gagctgtggt agcgtcctgt gcggtatatt tcccgttagc
    32101 aaggtcatag gcggccttta ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt
    32161 ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg
    32221 tgactgttcg tgctctgcaa ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt
    32281 ggtgtcaatg gtcagctcca ccgaggccac actgctgacg atgatgacca tgcgacaggt
    32341 ctgcgaacgc cctgagcctt cggcaagagc tggcttataa ctttcggcca tgttcgccac
    32401 ggcaattaac gttcccgcat catcgtacag gccaagctca cgcatccaga aaccgcccac
    32461 ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt cctggctgat
    32521 tttgttcagc gtatgtcgcc agacttcgtg gataagcccg gtctgtccgg catccgggac
    32581 aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct tccgccctcc
    32641 cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg ttctgaattt
    32701 tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg tcgtaagcca
    32761 caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca ataagatgac
    32821 ggcttgcagg tttggcatca gcaataagcc gttccatttc ctgatacatt gcctctgtga
    32881 tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc
    32941 accactccgt cacgttgatg agatagccga gcggctccac cacacgccgg attgcgccga
    33001 tagtgccttt atgacagtgg atgaaatagg catcgcggat aacggcgcgt tttgtcgctt
    33061 ccggccactt ttcatcccac ctgtcgaccg aaaacgccca cgccagccac ggcagcagat
    33121 ttgccggaca ggtatccggg ttccacagtt cacgaatcct gaccggcgtt ttttcaattt
    33181 ccgcacaggc ttttgcggcg gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg
    33241 aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta
    33301 ctgttgagca cgatgtcggc cagcggtgca gtcagttcga cacgctgcac gccttccaca
    33361 tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg
    33421 atgtacgctt ccagtttttt cacggcagca gcgcgtatgg gttcgctttc gggaccaggg
    33481 taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg cggcagactg cacggtcacg
    33541 cggtcggcca ccggcctgac gtcctcacca ttaagggcgt tacgcaccac cgccagcagg
    33601 tcttcggatg cgacaccgtt attttcacgt gacagcacag agatggtgac gcaggccgga
    33661 gacggactgg ttacagaaat atccgcgaca cgcccgtcgg cactgcgacc atgatactga
    33721 taggccccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat acgcagacga
    33781 taatccgtgt cagattccat cactgccggt gtcggcggga gggtcgaatc atctgccggg
    33841 gtgataatca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc attaccggcg
    33901 gcacaggcca gcattaccgc ccgtgcggcc tcattcacac gctgacgcca gataagctca
    33961 cgataagcat tttcctccag cagtttgacg agaggctcgg attccagcgt cagggtacgg
    34021 gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca
    34081 agaatggttt caaagtccag ctcctcgacc acatccggtg cgggtagctg gttcaggtcg
    34141 ataatcggca tggtttcaac tcacagggat ggttaacgaa agtggctggc cggtgtcgtt
    34201 gtgctggccg gttaacgtga ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga
    34261 tgacagggtg acgcgggctt cccatttcag cacggccatg taacaggcga ccttaatctg
    34321 caactcaagc gccggggtct gcggctggtc aatcattgac gccagcaacg agccgtaatc
    34381 acgacgcatc acccgtgagc cgaccggcgt gcgcaggata tcgccaatac tctggctgat
    34441 atgctcaagg tcagagacag tcaggccatc actgcgattc attccgagat aacgcgctgt
    34501 catagaggac tcccggttgt gccgccgctg tcgccggggt gtttatgggt atgcagtacc
    34561 ttcccgtttg atgagagttc accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc
    34621 ttctgcactt ccagcgtgcc agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc
    34681 agagtgacgc gggttgatgc tttcaccgtg accaccggca ccgtggcagt aacagaatca
    34741 gaagccgtca cgctggccgt tttaattccg cttaccgtga gtgcactggt ttcaggttca
    34801 tactcaatca ccgccccgtc agggaaacgg atatgcaggg catccgccga cgcagacggc
    34861 gcggggttat caccggaata aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct
    34921 acggccagaa tcagcacctg ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg
    34981 gcgcgatggg tcagccactg aagccagtcg gtgcacatgc cgccggtctg cacacggcag
    35041 cgaccggcat taaggtcggt ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg
    35101 cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat
    35161 accggcggca atgtgacgat gaggtgtcag gaatggcaca actaacggtc gaggtgagcc
    35221 aggataatct cttcaatcat ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc
    35281 ggataatgaa ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca
    35341 ctggctattt tcggtgactt cccgccgtaa aactccattg atgcctgttc agggctggcg
    35401 cggatatgca aaaaacgact ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg
    35461 gtcttttttc tggcgctctg ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc
    35521 tgtgccatca cccgacgctg ctgactctga cgcagacgtt tcgccagttc ggcgctcagt
    35581 cgccgacgcc ctgacggtga cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc
    35641 ttaaactcat tcatcccact tactcaccag ttcgccattg atataaagct ccatcgggcg
    35701 ggtgaccggc tccggcggcg gaggttccgg gatattcttc acatgcagcg cgccgtccac
    35761 ctcactgacc agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc
    35821 attgttgatg tctgcataaa acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat
    35881 gtcgggctga ttttcccgca gccacgccag caccggcacg atgagcaggt caaaatcacc
    35941 ggtaaagtcg gtcacaatca cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc
    36001 cagtgtggag gcaatactcc cgttatccac gaatatccgc agcatctcgg ggcttgtttt
    36061 cagcaccgtg acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt
    36121 cgtcctgaca gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa
    36181 gctgccggat atcagcactt aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc
    36241 aaaggctcac tttcgggcag gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc
    36301 tggtgcaacc ggcgcacagc atcaggcagg tcagcaccgt accagcggcg aaaatcttcg
    36361 ttttcattaa gtaacctcgt gatggttttc tcgcgctgag cttcacgctt cgcggcgttc
    36421 tccagttcct gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagggca
    36481 acatgaagct ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc
    36541 gccctgtcca gcaaggcgcg caggctggca tttttgtgtt tcaccagaaa cagaccggcc
    36601 accgccagcg ataacaacac aaccagcaca atcatcagtt ttgacatggt tcccgcccct
    36661 caagacgctg acggcaggcc gtacgtatca gccggaaaaa cagcgatgcc acgagataaa
    36721 tcagtgcggt aaaaatccac ccggcagcga ccagcgagat aaacgtcgcc accatcacca
    36781 tcagagccgc tgcccgtctg cgccacggca ccggctgcaa aaacagcgac gtgacaatct
    36841 tcacggccag cgattccggc ggcagctccc gcccgtagcg ttccagcaca tactccgcgg
    36901 catacacgcc gacaccaccg gcaatcacac agataaccgt cgccagaatc gcccatgcag
    36961 cgacaaaact gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca
    37021 gcgacacgtt cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc
    37081 cggtacgccg ccagcggtgc gccagacggt gaccagtttt tccagtgaat gctcacgctg
    37141 accgtaaccg gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc
    37201 aatgcgtccc gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag
    37261 cctgtcctgt gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca
    37321 gaacaggtaa agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa
    37381 caccttcgcc ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg
    37441 cttcccgtcc agtccggtga ctatcacgtc atagccccgg tttttcgtca gcggatgatt
    37501 cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct
    37561 aattaccggc attacggttt ccccctgccc ttaaagcggc gctgaatggc aatctcaatc
    37621 acctgataac cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg
    37681 tcaggaaact gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg
    37741 cgcccgataa acagacgcgg agtgatgggt tcaccaccgg caagcacctt gccgacaaca
    37801 atcagcaccc caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac
    37861 tcccacagat tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg
    37921 gtgccgtgtg gcagcaccgc ccccagttca gccagtcccg gatttgcggc gagcacggcc
    37981 tcgaccacgc cctcagtgcg cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc
    38041 tgtagcgcaa aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac
    38101 gcgccaccgc ccagcgcata tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg
    38161 ccttcttgtc gcccttggca ctggcatcca cgccgcggta acgctcataa agcgacgcgg
    38221 tcgccatcgc acacacagcg cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt
    38281 cgtccgccgg gacgtcagcc agacgcgaaa aaccggcagc aattttctgt tcgcggtact
    38341 cgtacagctc cgcattcgtc tccgccatgc ctgacttgat ggcctcacgc agacgggcgg
    38401 gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg cttcgggtca atatcgggaa
    38461 aaaagaacgt gtttttaatc accggctcgt cgcctgccgg ttgcgggata accaccgtac
    38521 cctcaccgga cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg
    38581 aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg
    38641 ccgccctggc gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt
    38701 ttgccggtgt cacgctccgg gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct
    38761 ccggcttcgg tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac
    38821 agtcgagctg tgtcgcacgt tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac
    38881 gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg
    38941 tggcggtcag ttcgagggtg tccgtcagca ggcggggatc cacagactca ccggcagcgt
    39001 gagcgcgcat ggccgcaagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt
    39061 gtttacccgg catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg
    39121 caatatcgcc ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac
    39181 ctttgccctg ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgtt
    39241 ttttcgcggc cttcagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca
    39301 gcttgaccag catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg
    39361 cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg
    39421 ctcccggctc tgcggtcgct ttagccggtg tggagaaatc accgacctta attttttcca
    39481 ccagacaacc ggcggcgtag tcctccacca cgtaatcaat gttcattgac tcgtagttct
    39541 ccacgcggtc gagtttcggg ttttccacaa tcacgcggcg atggctgtca tccatgtagt
    39601 agatggacag gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc
    39661 gtaccgccgg caggttaccg atgcgtttct ggctgatgat gacgtcagcg gccagcatct
    39721 cgctgttatc ctgctccttg ttgacgatag ggaaatactt gtccgccagt agctgacgcc
    39781 ccacaatcac cacaaggtca gggtcttcct gataccaagg ttcaatcagg ttgttggtcg
    39841 catccatcac cagtgcatcg aggcttacat aatcaccgcc cttacccacg cggataacct
    39901 cagaggtggt gcggccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat
    39961 tcggtatttc tgcagccagc cgaccgccac atcctgcagc atcggattac tgttgcggtc
    40021 agaggtttcg gcacgcttca cgccgttaaa accggccatg atgaaatcaa gggactggcg
    40081 tttgataatg gcgttacgga tacggagctg gaaatcctga taacgcgccc acaggtcaag
    40141 cgttttgtag cggatataaa aatcgaagtt aacctggtcg cattcgtact tgtttgacgc
    40201 cagcttcgag aagtccttcg gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc
    40261 gatggagccg gtgacaccga tgccaatttt ttcccctttc atttcactga ccggcacaat
    40321 gttgatgcgg gtcagaaagt cagaggactc ctgcatggtg ttcatcaggg tctgggtgac
    40381 cgacggttca acggtgaatt ttttcgacac atcaccggcg tcgatgccgt tcagctcggc
    40441 aacacgggac aggtaagcat taaatttaaa gcgggtttcc tggcgcatag tttttcctga
    40501 aattaagggt taatcgtgaa ggttttcccg gactgactga cgccggtcag cagttcgtca
    40561 tcagggcgtc accgccacca ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    40621 tgtggtcgag actgtttttc aggcgggtga atgcctggct ggtttcatcc gccctgtcag
    40681 tcacctcctg cttaagtgca gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg
    40741 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    40801 gacgggcatc atcgctggcc tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg
    40861 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    40921 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    40981 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    41041 aacatttgcc ggtgttggca aagttcggct gaatttccat tgaggtgtag accttctgcg
    41101 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    41161 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcggcc acatcgccat
    41221 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    41281 catagacacg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    41341 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    41401 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    41461 tgcgcatcct ccgccatcag tcccggatgg tttatcactg acacaacagc acattagcga
    41521 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    41581 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    41641 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacggtgcag
    41701 agctggaaac agcgcgacgg ctgggacagt gttgccccca tcagccgtgt cgaaatgagt
    41761 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    41821 gaaattgacc tgctcggacg ccagattgaa cgactggcac gggtaaaccg ctacagccag
    41881 accggcaacg aggcagacct taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc
    41941 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    42001 gagcagtctt tcgactatca gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc
    42061 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    42121 cgcgccctga aaaccggcca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    42181 gtgttccgtg aatacatcat cgcctttgct cgtctggttg acgttgacct gaccggtgac
    42241 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    42301 gcacagagcc ataacggcga cctgtacgtc gacgagattt tctggatccc gaattttcag
    42361 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    42421 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtcgggtga actgttcaac
    42481 cggggacgcg ccagcgccgc cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc
    42541 ggtggtcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgccctg
    42601 aaaggcggct gcacattgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    42661 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag
    42721 gagctgcaac gctgcatggt cgacacgctg gaagaatggg aagactacgc gccgtttgcc
    42781 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    42841 agcgccggat gcgtggtgct ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    42901 gagcgtcacc agtggaaagg catggacttt gccacccagg cggagtccat ccgcaaactc
    42961 accgaaaaat acaacgtcga atacatcggt attgatgcca ccggcctcgg tgtcggcgtg
    43021 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    43081 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    43141 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    43201 ggacgcagtg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    43261 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    43321 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    43381 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    43441 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    43501 ggtatgagcc gccggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    43561 acagctcacc gatttacgtc aaacgtaata ttctggcttc aacgtttatc ccgcacccgt
    43621 ggctttccca gcaggatttc agccgttttg tgctggattt tctggtgttc ggtaatgcgt
    43681 ttctggaaaa gcgttacagc accaccggta aggtcatcag actggaaacc tcaccggcca
    43741 aatatacccg tcgtggcgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    43801 cgacagcctt cgcgcccggc tccgtgtttc acctgctgga gcctgatatt aatcaggagc
    43861 tgtacggcct gccggaatat ctcagcgccc ttaactctgc atggctgaat gagtcggcca
    43921 cgctgttccg ccgcaagtat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    43981 ccgatgccgt gcaggatcgc aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga
    44041 aaggccgcaa taactttaaa aatctgtttc tctatgcccc acaggggaaa gccgacggca
    44101 ttaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    44161 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg gggggcaagc
    44221 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    44281 agcttatccc gttacaggac aggatccgcg agataaacgg ctggctcggt caggaggtca
    44341 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    44401 gcttttttac accccgccat cacgccctca cacgctcatc accgcacaaa aaatcccgca
    44461 gacacaccaa cgcccaacgg gcagactaag cgccgtcacg acgcgctgag acgctggaaa
    44521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    44581 gggcggtttt aatgcagttg catgaacact ccaagagcac gccagcgcta gtgataacta
    44641 acaataaaag cgacttctga cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc
    44701 tatgcggtat gctatccgct tcaattttta acacttgtta agggaactag acatggacat
    44761 tattgattta aaatctcaac tagaaaatga agataatgcc aagaaaagag cagagttgct
    44821 tgctcagcta gaggtgataa agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga
    44881 tgtggatttt gagaccaaag aatttaccgt tgagttatta gttaataagt atcataacgg
    44941 attagaggat gatactaacg aactctttgt tcctgattat caaagggatt ttgtttggag
    45001 tgaaaaaaga caatcaaaat tgatagagtc tttgattttg ggttttccta taccatatat
    45061 atttatagca gatgttctct ccgaagatcc cgatttagat gggcgaattg agattgttga
    45121 tggctcacaa cgagttagga ctatacacgc ttttatccaa aatgaattag ttttacaaga
    45181 cctgaaatct ttagacgtcc ttaatggttt caaattccaa gatttaccat tatcacgtca
    45241 acgtcgtttc atgagaattc ctgtccgcgt tatagaattg agttctaaat gcaatgaaga
    45301 aacccgtaga gatctttttg agcgtattaa ctctggcagc gatatattga aagatatgga
    45361 agtccgtaaa ggatcagaac ttggttcaac aaatttatat acacaggtta ttaagccatg
    45421 ctcaacaata cctaaattta aagaattagc acctttatct cagactaaag aaaaacgaga
    45481 tgaacggtta gaatttacat taagattctt tgcctacgta gataagtaca agaactttga
    45541 tcattccgta cgagattttc taaatgacta tatgtcagaa aatcgagata tagacgaaac
    45601 taaacaaaac tcaatgaaac aagagtttgc taatgttcta tcttttgttg aaaactattt
    45661 cccagcagga ttcaagaaaa ctattactgc aaaatctaca cctcgagtta gattcgaatc
    45721 tctcgctgtt ggcgcagcac ttgcattacg tgaagtccct gaccttgttc ccaagaactt
    45781 agattggcta agctcagaag aattcaaacg attgaccact tccgacggag ctaatagtcg
    45841 cgtgaaggta attgaacgaa ttgaatatgt tcgggataaa ttattagagg attaacatgg
    45901 atcgttttcg tgaggatttt gacgaaaggt caggagagat cttggcctat ttggatcttt
    45961 tgaaatttat tgagtatgcc ggtgcagaac tgatatcagc tgatgataaa gaacataagt
    46021 tctccattac tgctcaatca cgaaaaactc taaagggagc agtatatata ctgttatata
    46081 acct
//
LOCUS       DAZPLM010000010        43399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPLM010000010 DAZPLM010000000
VERSION     DAZPLM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.010"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHVC7_000481"
     tRNA            complement(2..94)
                     /locus_tag="ACHVC7_000481"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHVC7_000482"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHVC7_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR2114623.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHVC7_000483"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHVC7_000483"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR2114624.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHVC7_000484"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHVC7_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR2114625.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHVC7_000485"
     CDS             complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHVC7_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR2114626.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHVC7_000486"
     CDS             complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHVC7_000486"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR2114627.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHVC7_000487"
     CDS             complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHVC7_000487"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR2114628.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7440..8003
                     /gene="srlA"
                     /locus_tag="ACHVC7_000488"
                     /gene_synonym="gutA"
     CDS             7440..8003
                     /gene="srlA"
                     /locus_tag="ACHVC7_000488"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR2114629.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8000..8959
                     /gene="srlE"
                     /locus_tag="ACHVC7_000489"
                     /gene_synonym="gutE"
     CDS             8000..8959
                     /gene="srlE"
                     /locus_tag="ACHVC7_000489"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR2114630.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            8970..9341
                     /gene="srlB"
                     /locus_tag="ACHVC7_000490"
                     /gene_synonym="gutB"
     CDS             8970..9341
                     /gene="srlB"
                     /locus_tag="ACHVC7_000490"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR2114631.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9345..10113
                     /gene="srlD"
                     /locus_tag="ACHVC7_000491"
                     /pseudo
     CDS             9345..10113
                     /gene="srlD"
                     /locus_tag="ACHVC7_000491"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10219..10578
                     /gene="gutM"
                     /locus_tag="ACHVC7_000492"
     CDS             10219..10578
                     /gene="gutM"
                     /locus_tag="ACHVC7_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR2114632.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10645..11418
                     /gene="srlR"
                     /locus_tag="ACHVC7_000493"
                     /gene_synonym="gutR"
     CDS             10645..11418
                     /gene="srlR"
                     /locus_tag="ACHVC7_000493"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR2114633.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHVC7_000494"
     CDS             11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHVC7_000494"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR2114634.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHVC7_000495"
     CDS             complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHVC7_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR2114635.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14074..15513
                     /gene="norV"
                     /locus_tag="ACHVC7_000496"
     CDS             14074..15513
                     /gene="norV"
                     /locus_tag="ACHVC7_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR2114636.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15510..16643
                     /gene="norW"
                     /locus_tag="ACHVC7_000497"
     CDS             15510..16643
                     /gene="norW"
                     /locus_tag="ACHVC7_000497"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR2114637.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="ACHVC7_000498"
     CDS             complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="ACHVC7_000498"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR2114638.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTISQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="ACHVC7_000499"
     CDS             complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="ACHVC7_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR2114639.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="ACHVC7_000500"
     CDS             complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="ACHVC7_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR2114640.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21121..22578
                     /gene="ascF"
                     /locus_tag="ACHVC7_000501"
     CDS             21121..22578
                     /gene="ascF"
                     /locus_tag="ACHVC7_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR2114641.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22587..24011
                     /gene="ascB"
                     /locus_tag="ACHVC7_000502"
     CDS             22587..24011
                     /gene="ascB"
                     /locus_tag="ACHVC7_000502"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR2114642.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24125..24544)
                     /locus_tag="ACHVC7_000503"
     CDS             complement(24125..24544)
                     /locus_tag="ACHVC7_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2114643.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24541..24852)
                     /locus_tag="ACHVC7_000504"
     CDS             complement(24541..24852)
                     /locus_tag="ACHVC7_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR2114644.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25014..25787)
                     /locus_tag="ACHVC7_000505"
     CDS             complement(25014..25787)
                     /locus_tag="ACHVC7_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114645.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="ACHVC7_000506"
     CDS             complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="ACHVC7_000506"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR2114646.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="ACHVC7_000507"
     CDS             complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="ACHVC7_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR2114647.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="ACHVC7_000508"
     CDS             complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="ACHVC7_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR2114648.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="ACHVC7_000509"
     CDS             complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="ACHVC7_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR2114649.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="ACHVC7_000510"
     CDS             complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="ACHVC7_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR2114650.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="ACHVC7_000511"
     CDS             complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="ACHVC7_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR2114651.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="ACHVC7_000512"
     CDS             complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="ACHVC7_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR2114652.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="ACHVC7_000513"
     CDS             complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="ACHVC7_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR2114653.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="ACHVC7_000514"
     CDS             complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="ACHVC7_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR2114654.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33874..34224
                     /gene="hypA"
                     /locus_tag="ACHVC7_000515"
     CDS             33874..34224
                     /gene="hypA"
                     /locus_tag="ACHVC7_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR2114655.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34228..35100
                     /gene="hypB"
                     /locus_tag="ACHVC7_000516"
     CDS             34228..35100
                     /gene="hypB"
                     /locus_tag="ACHVC7_000516"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR2114656.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35091..35363
                     /gene="hypC"
                     /locus_tag="ACHVC7_000517"
     CDS             35091..35363
                     /gene="hypC"
                     /locus_tag="ACHVC7_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR2114657.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35363..36484
                     /gene="hypD"
                     /locus_tag="ACHVC7_000518"
     CDS             35363..36484
                     /gene="hypD"
                     /locus_tag="ACHVC7_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR2114658.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36481..37491
                     /gene="hypE"
                     /locus_tag="ACHVC7_000519"
     CDS             36481..37491
                     /gene="hypE"
                     /locus_tag="ACHVC7_000519"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR2114659.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37565..39642
                     /gene="flhA"
                     /locus_tag="ACHVC7_000520"
                     /pseudo
     CDS             37565..39642
                     /gene="flhA"
                     /locus_tag="ACHVC7_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39679..40032)
                     /locus_tag="ACHVC7_000521"
     CDS             complement(39679..40032)
                     /locus_tag="ACHVC7_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR2114660.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40109..40243)
                     /locus_tag="ACHVC7_000522"
     CDS             complement(40109..40243)
                     /locus_tag="ACHVC7_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114661.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40319..42880
                     /gene="mutS"
                     /locus_tag="ACHVC7_000523"
     CDS             40319..42880
                     /gene="mutS"
                     /locus_tag="ACHVC7_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR2114662.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42986..43399
                     /locus_tag="ACHVC7_000524"
                     /pseudo
     CDS             42986..43399
                     /locus_tag="ACHVC7_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10273 a        11917 c        11305 g         9904 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gagtgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcacca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcagc caattcgtta gaggatcgag
     4201 gtgagagtaa tttttcacgc cagtgttggc ctgttcccct caccctaacc ctctccccaa
     4261 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4321 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4381 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4441 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4501 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4561 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4621 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4681 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4741 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4801 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4861 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4921 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     4981 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttat
     5041 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5101 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5161 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5221 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5281 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5341 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5401 ataccggata gtcaatatgt tctgttgaag caattatact gtatactcat acagtatcaa
     5461 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5521 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5581 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5641 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5701 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5761 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5821 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5881 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     5941 tcactgtcag tcatcacaac ctcagtcagt caaaaattta tgccggacaa gatagcactt
     6001 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6061 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6121 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6181 atggtgtaga agttcggcag accgtaccag tactggtaac cggtaccgac atccagacgc
     6241 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca
     6301 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6361 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6421 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6481 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6541 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc
     6601 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6661 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6721 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6781 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6841 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6901 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     6961 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7021 gcgaaatcgc cgcccatctg catcacattg tgctgaggct caagcaggaa gccgccagac
     7081 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7141 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7201 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7261 ctgcccgata cggactttac ataactcaac tcattcccct cgctatcctt ttattcaaac
     7321 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7381 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7441 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7501 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7561 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7621 ccggtaaccc tgtttcccgt tacctgctgc taccgtgcat tggcacgttt gtcttttgca
     7681 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7741 cggcagcctc ttatagctgc cactcaatga atggcctctt tccccatatc aaccctggcg
     7801 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7861 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttctttcgc ggctgggtga
     7921 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     7981 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8041 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8101 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8161 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8221 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8281 caagtccagt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8341 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8401 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8461 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8521 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctc attggcatca ttatggcttc
     8581 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8641 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8701 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8761 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8821 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8881 tgtactggtg agccactttt taaccggcgc accgactgta ctgatcgcct ggtttgtctc
     8941 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9001 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9061 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9121 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9181 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9241 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9301 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9361 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9421 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9481 ttaacgccga atatggtgaa ggaacagcgt atagttttgg tgctgacgcc accagcgagc
     9541 aaagcgtcct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9601 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9661 accgtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttctcgttt gatgatccgc
     9721 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
     9781 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
     9841 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
     9901 tcgcctatgt tccagtcact gttaccccaa tacgcgacca agctgggtat caaaccggat
     9961 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggat
    10021 gtgctgaata tgctgctgct ctatgccagt cctaaggcgt cgtactgcac cggacagtcg
    10081 atcaatgtca ccggcggtca ggtgatgttc taatcaacag cggagatccg ttaaggatct
    10141 ccgtgagact aatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca
    10201 ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt
    10261 gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta
    10321 tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc acgggtcgtg
    10381 gtcgccatcg ccctggacga tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg
    10441 accgtcttcg cacgaccgca aaaaattccc gcaattaccg gtatgcatgc gggtgattta
    10501 cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt
    10561 aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga
    10621 aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag
    10681 tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca
    10741 ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt cattcgtact
    10801 tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacactc
    10861 atcaacaccc acaagaaaga gctgatagca gaagctgccg ttagttttat tcatgatggc
    10921 gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct gctctcgcgc
    10981 tttaataaca tcacggtgat gaccaacagt ctgcatatcg tcaatgcgct atctgaactg
    11041 gataacgaac aaactatcct gatgccaggc ggaacgtttc gtaaaaaatc ggcctcattt
    11101 cacggacagc tggcagagaa tgccttcgaa catttcagtt tcgataaatt gtttatgggc
    11161 accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggttta taccgtcagt
    11221 aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc
    11281 cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctgat taccgacgca
    11341 ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc
    11401 ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa tgctggagtt
    11461 gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat
    11521 catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg
    11581 taagaaaatc gccgcaacac ttgccagtac cggcacatcg gctttttttg tccatccggc
    11641 agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc
    11701 ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat
    11761 cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt
    11821 gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac
    11881 cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt
    11941 taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc gcttgctgaa
    12001 taaagtgcat catctgatgc gccgtgacga tgccatccca caggtggcgt taaccgccag
    12061 cgtgatggat gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga
    12121 cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg
    12181 cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacgttaca
    12241 ggcgcaaagt cgcgccatcg acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc
    12301 accggtggtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca
    12361 ggccgggatt atttaatcct tcattcccag acgtttcgcc agccgatgca ggttggcgac
    12421 gtcggtttcc agcatccgcg cgcaggcagc ccagttatga tgattttgtg ccagtgcctg
    12481 gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg
    12541 caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc
    12601 aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc
    12661 atgttccagt tcgcgcacgt tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc
    12721 ggcacttaat accacgcggg agagccccag ccgcaaacga cactgctcgc agaaataccc
    12781 cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac
    12841 gctcaggcga tgaaacaagt cagcgcgaaa tcgccctgcc atcacctctt cgcgtaagtc
    12901 gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg
    12961 ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc
    13021 gccgatctca tccagaaaca gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt
    13081 actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact
    13141 ttccggcagt gcagcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc
    13201 atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcaggacgtt
    13261 gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa
    13321 gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt
    13381 ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcagc
    13441 aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag
    13501 cgtcagtgcg ccgatcaggt tttgcccggc aaacaatggc agaccaacgc aggcgtgaac
    13561 cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc
    13621 gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag
    13681 cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg
    13741 ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt
    13801 gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt
    13861 cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt
    13921 gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc ataattttat
    13981 ttatagagta aaaacaatca gataaaaaac tggcacgtaa tctgcaatta gcaagacatc
    14041 tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa aaataacatt
    14101 cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacgga atataaaacg
    14161 ctacgcggca gcagttacaa cagctacctc atccgcgaag aaaaaaacgt gctgatcgac
    14221 accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg
    14281 gcggatatcg attacatcgt gattaaccat gcagaagagg accacgctgg ggcgctgacc
    14341 gaactgatgg cgcaaattcc cgatacgccg atttactgta ctgccaacgc tatcgactcg
    14401 ataaatggtc atcatcatca tccggagtgg aattttaatg tggtgaaaac tggtgacacg
    14461 ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa cgccaatgct gcactggccg
    14521 gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga tgctttcggt
    14581 cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga gcttttcgag
    14641 cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt aacaccgaaa
    14701 attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc tcacggcgtg
    14761 gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc ggctgattat
    14821 caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg catgatggct
    14881 gacgctatcg cccaggggat tgcggaaacc gacccacgcg tggcggtgaa aattttcaac
    14941 gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg
    15001 gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccggtct ggtggaggag
    15061 atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggctctca cggctggagc
    15121 ggcggtgcgg tggatcgtct ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt
    15181 agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg tgaacacggt
    15241 cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt gaatacggtg
    15301 gttaaagaag aaacctctgc caccacgaag tccgacctcg gcccacggat gcagtgcagc
    15361 gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga
    15421 acgccgtgga gtgaagttcc ggataacttc ctctgcccgg aatgctctct cggcaaagac
    15481 gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc attggttcgg
    15541 gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccagt attccattaa
    15601 ccctgatcgc cgccgacagc atggatgagt acaacaaacc ggacctcagc catgttatca
    15661 gccaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt gccgggcagt
    15721 ttaatctgca cctgtttccg cacacctggg tgacggatat cgatgccgaa gcccatgtgg
    15781 tgaaaagcca gaataatcag tggcaatacg acaagctggt gctggcaact ggtgccagtg
    15841 cctttgtccc gccagtgcct gggcgtgagt taatgctgac gttaaatagt cagcaagagt
    15901 atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc ggcggtggtt
    15961 tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc acgctaatcg
    16021 acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc cgcttgcagc
    16081 atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag gggctggaaa
    16141 aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg
    16201 taattgccgc caccggactg cgcccggaaa cagccctggc acgacgcgcc gggctgacga
    16261 ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgacgat atttatgcgc
    16321 tgggcgattg cgcggaaatc aacggtcagg tactgccatt cctgcagccg attcaactta
    16381 gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa ctaccggcga
    16441 tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa acccagcgtc
    16501 aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggctcgc ggtgttgacg
    16561 atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag gcatttggat
    16621 tgttgaaaac attgccgatg taggtgggct actgtgccta aaatgtcgga tgcgacgctg
    16681 gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcgt ttacgccgca
    16741 tccggcaatg gtgtccaaat gcaacacgtt ttatccgttc tggacttcac ccgctaacca
    16801 acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact
    16861 ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg
    16921 gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc
    16981 acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt
    17041 aaccggtgcc tgccagttca gccactgctg ccagaaagcg gcgagatcca gttgattgtc
    17101 caccagcggc atcgtcaccg gatgcgtcac tccgtggcat gaggctgcga gcgcctccag
    17161 agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc
    17221 cactgcatcg aacaaccgcc cacacgatga cgccagcggc gcgttaattc cacgctcaat
    17281 ggcccgcgcc agcacgctcc agttttgctg ttgcacactt gctgtttcag ggtaattctg
    17341 ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcttcgctgc
    17401 caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata
    17461 gttcacccgc aggcactcgc cgccccacaa agcgccgttc tcccccatac cgataccgtc
    17521 gagcgtcaaa gcaatgacat caccgccatc caacggccac tgatgctctg ccagacacgc
    17581 cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt cacgcgccca
    17641 ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcgggg taaaatcgta
    17701 gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa
    17761 atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtattttt
    17821 cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag gcaaagccag
    17881 cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga
    17941 atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg
    18001 caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga
    18061 ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg
    18121 caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct caggaacgta
    18181 ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc
    18241 tggtaaaccg tcagccactg gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg
    18301 cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc
    18361 aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagccg cctgtaatgc
    18421 cgcctcttgt tctgcatgtt caccatgact tacccattca agatgtgggc cacactccgg
    18481 gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacggtact ctttgtcaca
    18541 agccggacac aacggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg
    18601 aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg
    18661 ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtagcggcat ccgggacaat
    18721 ttgcgtattc atggcacctc ccgcgctctg gctgatggtg aactcagtgg gcagttgtga
    18781 ccagatgtac ggctcacgct cgacgctatc aatacgcgcc agcggcgggc agtgctgatg
    18841 caattgaaca agtaacgttt ccgggtcttc ccgcagccgg acttctacgc catcgccgtc
    18901 attacagaca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa
    18961 accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg tgttttttgc
    19021 cattgagtta ttcccgccat catgaattgc gtaacccgtc ctgccggaca cgactgcgtc
    19081 gtatccggca gtctcaggtc ggcgataccg ccagctccgt attctacgaa tatttccggg
    19141 aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc cgtacggcgg
    19201 cgtttttctg cactcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgagtc
    19261 gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc acatttattg
    19321 gcttcggctt tgtcagcccg tacattcagc cccgcgccgc tgttgcgaat caccggacgt
    19381 accaccactt ccatcgcgcc atacgggcaa gccacaacgc aggttttgca accaatgcaa
    19441 cgttcctgca tcacatgaac aaacccttta tcacggctga tagcaccatt cgggcagacg
    19501 ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat gttcacacct
    19561 ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca gtcctgattt
    19621 tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg
    19681 tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca
    19741 ttcacaaatc atgccagttt taattcactg ttatttaagg aaattaattt ctgtaatgca
    19801 ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa
    19861 agagcaatga gtgagtcgcg gcggatcagt tttccgctga aggttttcgg cggtgagaaa
    19921 tccccgccat cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc
    19981 ggaattttta cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat
    20041 ccgataactg acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca
    20101 cctatcgcca tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc
    20161 aacgtttcta ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt
    20221 tcattgagcg caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg
    20281 ctggtggggg aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc
    20341 aactctgcca cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg
    20401 tttttgcgca ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca
    20461 tcgatctcat ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc
    20521 agcagatact gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc
    20581 aactgccgcc ctttctcttc cgccattcgc gcggcatgaa agagtaattc actaaaataa
    20641 atgccgtggt aaagcgtgtt ggtcactacc aaccccagcg tctgagtact cttcgccgac
    20701 agattgcgcg ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga
    20761 tctttagtct cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt
    20821 gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac
    20881 gcaatcaacg cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa
    20941 aattcaggtg accggtttca caaatataaa aaatgaacaa ttaactctct tgcttattta
    21001 gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt
    21061 tttccgttac gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg
    21121 atggccaaaa attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat
    21181 aacatctcgg cggtcacgca ctgtatgacg cgtttgcgcg ttgttatcaa agatgaccag
    21241 cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac
    21301 aaccagtgtc aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc
    21361 ctgctgccgg gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt
    21421 cgcattggtg cggggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg
    21481 attatcggcg gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg
    21541 acaaaaggat cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc
    21601 ctgccgctga tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg
    21661 attgccatcg cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc
    21721 cagggtgaac atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca
    21781 gtgatcccgg cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc
    21841 attacaccgg cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca
    21901 ccgctggcaa tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg
    21961 ctggtttaca ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg
    22021 tggcctctgc tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc
    22081 attgccgaaa ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg
    22141 ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg
    22201 gcgctggctg cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc
    22261 gtggcgatcc gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc
    22321 gcggttgccg gtatggcggg gcttgccagc cactcaatgg cagcgccagg gttatttacc
    22381 agcgtgcaat tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg
    22441 gcgctggcgg tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct
    22501 gttgaggaag cggctgccga ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa
    22561 gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg
    22621 gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa gggctgacca
    22681 ctgtcgatat gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac
    22741 gttttcagtt gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc
    22801 gttataaaga agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa
    22861 ttgcctggag ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg
    22921 ctttttatcg ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt
    22981 tgtgccactt cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca
    23041 agctggtgga gtttttcagc cgctacgccc ggacctgctt tgaagcattt gatggtctgg
    23101 tgaaatactg gcttaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg
    23161 cgggtctggt gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc
    23221 accagctggt tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc
    23281 aggtgggctg tatgctggcg ggcggtaact tctaccctta cagttgcaag ccggaagatg
    23341 tctgggcggc gctggagaaa gaccgggaaa acctgttttt tatcgatgtg caggcgcggg
    23401 gcacgtatcc ggcttactct gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag
    23461 caccgggcga tgatgaaatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg
    23521 cctcgcgctg cgcctcggcg gagatgaacg ccaacaatag cagtgcggcg aacgtagtga
    23581 aatcgctgcg taacccgtat ctgcaagtga gcgactgggg ctggggtatt gatccactcg
    23641 gtctgcgtat caccatgaac atgatgtacg accgttatca gaagccgctg tttctggtgg
    23701 aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc
    23761 gcatcagcta tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc
    23821 cgctgatggg ctacaccacc tggggctgta ttgatttagt ctccgcctct acgggtgaaa
    23881 tgagcaaacg ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga
    23941 cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggggaag
    24001 atttagagta ggtaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc
    24061 acgaaccagg aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc
    24121 aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg
    24181 atatcgtcag ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt
    24241 cgttcttcaa atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca
    24301 cgccaacagg cattgccgca acggctttgt cgaactctgc aaactcttgg ttgttgtttt
    24361 catattctgc aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa
    24421 tcaaatattc gaccagttcc agtgcttcac gataatcatc ttctgagtca ctacctccca
    24481 gaaaggggac tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag
    24541 tcattctttg ttctctctgt agtaacgtgt cagtttgtca tactcggcgt gattagttat
    24601 gtgcttcaca aagaaccgct tattaacaaa gtttatgtag gcaatcaccc ttaggttgtt
    24661 cccacctaca tccagaacgt accatttgtt gcgatattta aaattgtcga ggttgggaaa
    24721 cgccgttcgc atctcttctg gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc
    24781 ctgaagagcc agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga
    24841 aatgatgtgc atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgag
    24901 ttctatcctt atgtgttcgg aaactatagc tcatcaaatc caagttcaca atatgtaaac
    24961 acttgagcgc gatataatta cccccaaaat cctcatagcc actctgcaaa aagtcagttc
    25021 tggttcaccc catcaagaac atcccccttt atcaagcaac gctgcgtctg cgccagatct
    25081 tccgtttgcg ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa
    25141 gtaaaacgcg tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat
    25201 ttggcgtctt ttacctgata cagactggtt atccagtaac cggaatcaaa ctgcatatcc
    25261 agcaattgcg tgtgaccatt gccttgcaaa tccagcaaat catctacgcc agttttgctt
    25321 gcggtgtaga atcccctggg ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata
    25381 agaattaaat gtgcataagg agccagacca ttaccggtac ttggtaacca gataaccaga
    25441 tcctgaatgc cgttacgatc aagatcggcg cgatagagtt gccctttgcc cgctatgttt
    25501 gccagcatac ccacaggcac agaccaggat ttaccggcgc gatctttacc gttcaattgc
    25561 cattcactat catcatttgg catcttttca agcgtcacgc cagcaacgcc cacttttcta
    25621 aaatgccagg tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt
    25681 tgcgcgtatt cgctgtcact cattggcaga tattcggtaa agctggtggc ctgtgcaggc
    25741 agcactaagc tcaataaaaa agcgcacatc cctgttctga tattcatttc gctgaattcc
    25801 ctgatggaga attattcttc ttccaccgct aactgcgcga agccgccatt cccttcccag
    25861 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgagt catcgggtag
    25921 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat gtcttctttc
    25981 aactggtcga taagatagtt cagcggcatg ttatgggtag tcatcataaa catctcggcg
    26041 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    26101 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgca
    26161 ctaccgccat caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    26221 agcgggccag cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    26281 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    26341 taacagcatt gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa
    26401 tgcgttggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    26461 gccttccatt ttgcggcggg cttcactgcc cgcctccaga gtggcaatcc atgccagata
    26521 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    26581 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    26641 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    26701 tcaacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    26761 agccagcgtg caacctgttc ttcgccctgc cctaactgtg tcaggaagtc atcggcaatc
    26821 tgacgaccgt aacgataacc cgccaggcgg cgtgcttcgc gatccacttt cacacgcagc
    26881 ggctgcacca tatcgccatg caggatttcc gcctgttgtt catcctgctc acccgggcca
    26941 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    27001 gcaggcgttg gcgggcaacc aggaatgtag acatctaccg ggacgatttt atccgtaccg
    27061 ccccacacgc agtagagatc gtggaaaata ccaccgctgt taccgcaggc accgtaggag
    27121 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    27181 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    27241 atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg cgaaaatttc gatttcgcag
    27301 ccgttgcagc cgccgcagtc cacgcgataa acgtaggcag aacgtttgat ttttttcagt
    27361 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    27421 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    27481 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    27541 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    27601 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc
    27661 gggactgttg caggaagtct tctttcttcc acactgccag ttcgtactct tgcgacagtt
    27721 tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca gaagatgcag cgcccgagat
    27781 taaactccca cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg
    27841 ggcaggcatt gacgcaggcc gcacagccga tgcactgctg cgggttctgc tccggcttac
    27901 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    27961 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    28021 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac
    28081 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtccaag ctaccgataa
    28141 tcagcggcgc atcggaaacg gtgttaccgc gcagcatgta gcgcagggtc ggccagttcg
    28201 cgtaggtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    28261 agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt
    28321 aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca
    28381 tgttcagcgc ggtataaact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    28441 cctgctcgct gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc
    28501 gagtatcgcg ggcgtgaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg
    28561 ccatttccgg gtccagacga ccaatgccga cggtgcgctg ttccatgttc ggcgtactca
    28621 gcagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    28681 ggatcatgtc atctttcaac agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    28741 ttttacgcgc accggtgagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    28801 actgcataaa gccggagtca aagccggtaa agtggcaggc gagaccgagg ttgagcagat
    28861 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    28921 cctgaatacc catcgcgttt tctaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc
    28981 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    29041 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    29101 cgccatcgac gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa
    29161 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    29221 cggtggtcgg tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    29281 catccggcag caccagacgg cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    29341 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    29401 ccggtttgtt ggcatcaact tcgacgcgaa ccgttaccca gcacttagtc cccttctcca
    29461 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaaa
    29521 gcaccgacaa ccagcccccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    29581 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    29641 ccggaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    29701 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    29761 cccagtccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    29821 cagcgcgata accaggacgc cgacgaccag tttgacgatg gcaatcacca gcgccagcag
    29881 cagcccaccg gcggtgaagg tttccatctg tccccacggg ataaatacgc cgacgaacat
    29941 ctgcagcacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctgcc
    30001 gctgtattca gagagcgggc cttcctgcag ctcctgctcg gcttccgcca ggtcgaacgg
    30061 cagtttgccc atttcgataa aggtggcgaa cgcacaggca caaagcgcca gtaccagcgg
    30121 gatgctctgg ctcagcggcc agtgataaac ggtgtcggtg atgttgctga tgttggtgga
    30181 accggcaacc tgtgcggcaa cccacagacc aagcagcagc atcggttcga ccagcacgcc
    30241 aagcatcgct tcacggctcg cgccgatagc ggtaaacggg ctaccggtat ccagaccaga
    30301 aatggcaaag aagaaacgcg cgatggcaaa gagatacagt aaggtgatta aatcacccag
    30361 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    30421 gcccaccatc acatacggcg tcaggcggaa cacccagccg gaggcatccg ggccaacgct
    30481 ctggcggccc agcagtttga aaatgtcacg atactcctgc aacacacccg gcccacgacg
    30541 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    30601 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    30661 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    30721 ccttcgcact gccagcccgg caccagcgac accggattca gccatttgcg cagtttcagc
    30781 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    30841 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    30901 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    30961 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    31021 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    31081 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    31141 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    31201 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    31261 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    31321 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    31381 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    31441 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    31501 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    31561 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    31621 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    31681 agcgcgatcc cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc
    31741 agcaggccaa ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta
    31801 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cggccagcag gccaaagcca
    31861 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagt
    31921 aggcacagat ccagcgtgcc ataacgctgc cacagcagcc agcaagcaat caccagcagc
    31981 agagtgccaa gacgccccag cgcaaaccac agtttgccct ctttgctgtt gctggtgagg
    32041 aacaccgcac acaaggccat gacttcggcc attaccacga acatgccgag gttgctggcg
    32101 atgacggcgc agacggcggc agccatcaac atattgatct gcaagccgtt gcacttcacc
    32161 tgcgcgtggc gatgccagtc aatgttgtag aggctgacaa acagaccgca cagaccgagc
    32221 gtaatcagcc aaatcgcgtt aagcggagag atttgcacat cgtagcttac cagcgacagc
    32281 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    32341 ctaccaaccg cgccgccaat tccagctatc cagccactta acgctttttg aaaagaaaag
    32401 agaaatgcca gaacagcggc ggcgacaaac catgccacgc cgctattgat cagggaaatt
    32461 gcgctcattt agcctctcca ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg
    32521 tgttaaaggt cagctcacgc ttacgtttgc tgacgcgggc gatatcggtg ttatccacca
    32581 gatgcagggc tttagtcggg cacatccgca cgcaggccgg accttgttca tcaaaactgc
    32641 aaaggtcaca tttgacggcg atcgcgcgaa tacccggcac ccagtcaagc agcgtactga
    32701 tacgcgccgg agcaggcggt gccggtggcg ctttcggggt attggcgttt gccggaatat
    32761 ccagcggacg gctgccggaa aattcaattg cgccaaacgg gcaggcgatg ccgcacagtt
    32821 tgcagcttac gcacagactt tcattcaact gcacggcccc atcaatgcgg gtgatggcgt
    32881 taaccgggca aaccaccgca cagggtgcat cttcacagtg gtgacagagc tgcggcgcag
    32941 attctttttc attcagcatc actctcaggc gcggcattga ttgcaggccg tgctggcgat
    33001 gcgtctctga acaggcggcc tcacaagtgt ggcagccgat acagagcgtg gagtcaacaa
    33061 ttacaaaacg attcaccagg cattcctcag gtgattgtca tttttgacga aaacatgccg
    33121 atgaaatgtc attttcgaca ctcatcgaca cgcccatccc caaacaggcg taacgcctgc
    33181 aaaacgggca aagcctcagc tcatgctgcc gggctttgtc cctttaccag ttggcttaaa
    33241 ttcaccggca cattgttttc aatagcggtg tataagctgt cgtaaacgcc agcgattttt
    33301 tcgagatagt gttccagaac ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg
    33361 ccgtcaatgc tcagttgcgc ctgcgcaatc cgctgtttgt cttcgccatc ttttgtttcg
    33421 acgtaatact cgatggtgtg gctgttaaag ccatccgcca gggtgacgta aatacgaaaa
    33481 tgttcaaaaa ggacgaaaca gagttctttg tccggcgcgc agctcatggc gtgatgcagg
    33541 cgcgctttcg ataacgtgat cccctgaacc agcgaattgc agtagatgtg ccactggtcc
    33601 tgtaggcgac gatgccgctg tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc
    33661 attgtcaggt tacccgttta acagagatgc cagctttaag cattttctat gccaactttt
    33721 aatttattgt tattaaatag atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt
    33781 gtcatttcga catcatcgac attattcacc gcagggataa tcaacactgg cacaattatt
    33841 gcttgtagct ggcaatagtt aatgggaggc gatatgcacg aaataaccct ctgccaacgg
    33901 gcactggaat tgatcgaaca gcaggccgca aaacacggcg caaaacgcgt aactggggtc
    33961 tggctcaaaa ttggcgcatt ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat
    34021 ctggtttgcc gcggcagcgt ggcggaaggt tgtaaactgc acctcgaaga acaagaggcc
    34081 gaatgctggt gtgaaacatg ccaacagtat gtgacgctac tgacccagcg cgtccgccgc
    34141 tgtccacagt gtcatggtga catgctacag attgtggcag acgacggttt acagattcgg
    34201 cggatagaaa tagaccagga gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac
    34261 ctgtatatcg agggtgatga acataaccct cattccgcgt ttcgtagcgc gccatttgcc
    34321 ccggcggcac gcccgaagat gaaaatcacc ggcattaaag cgcctgaatt tacccccagc
    34381 cagactgaag aaggcgacct gcattacggt catggcgaag cgggcactca cgcgccgggc
    34441 atgagccagc gtcggatgct ggaagtcgaa attgacgtgc tggacaaaaa taaccgtctg
    34501 gctgaacgca accgcgcgcg ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc
    34561 agccctggtt ccggtaaaac caccctgctg acggaaaccc taatgcgcct gaaagacagc
    34621 gttccgtgcg cggttattga aggtgaccag caaaccgtga acgatgccgc acgcattcgc
    34681 gctaccggca caccagcgat tcaggtgaac accggtaaag gctgccatct tgacgcacag
    34741 atgattgccg acgccgcacc gcgtctgcca ctggacgata acggtattct gtttatcgaa
    34801 aacgttggca acctcgtatg cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg
    34861 gtgctttccg ttaccgaagg tgaagacaaa ccgctgaaat atccgcatat gtttgccgcc
    34921 gcctcgctga tgctgctcaa caaagttgac ctgttgccgt atctcaactt tgacgttgag
    34981 aagtgcatcg cctgcgcccg cgaagtcaat ccagaaattg aaatcatcct tatttccgcc
    35041 accagcggcg aagggatgga ccagtggctg aactggctgg agacacagcg atgtgcatag
    35101 gcgttcccgg ccagatccgc accattgacg gcaaccaggc aaaagtcgac gtctgcggca
    35161 ttcagcgcga tgtcgattta acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg
    35221 tgggccagtg ggtactggta cacgttggct ttgccatgag cgtaattaat gaagccgaag
    35281 cacgcgacac tctcgacgcc ttacaaaaca tgtttgacgt tgagccggat gtcggcgcgc
    35341 tgttgtatgg cgaggaaaaa taatgcgttt tgttgatgaa tatcgcgcgc cggaacaggt
    35401 gatgcagtta attgagcatc tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg
    35461 ccctctgcgg attatggaag tgtgcggtgg tcatacccac gccattttta aattcggcct
    35521 cgaccagtta ctgcctgaaa acgttgagtt tatccacggt ccgggttgcc cggtgtgcgt
    35581 actgccgatg ggtagaatcg acacctgcgt ggagattgcc agccatccgg aagtcatctt
    35641 ctgtaccttt ggcgacgcca tgcgcgtgcc ggggaaacag ggatcgctgt tgcaggcaaa
    35701 agcacgcggt gccgatgtgc gcatcgttta ctcgccgatg gatgcgttga aactggcgca
    35761 ggagaatcca acccgtaaag tcgtattctt cggcttaggt tttgaaacca ccatgccgac
    35821 caccgccatc accctgcaac aggcgaaagc ccgcgatgtg cagaattttt acttcttctg
    35881 ccagcatatt acgcttatcc cgacgctgcg cagtttgctg gaacagccgg ataacggtat
    35941 cgacgcgttc ctcgcgccgg gccacgttag tatggttatc ggcactgatg cctataattt
    36001 tatcgccagc gattttcatc gtccgctggt ggtcgctggt ttcgaacccc ttgatctact
    36061 gcaaggcgtg gtcatgctgg tggagcagaa aatagcggcc cacagcaagg tagagaatca
    36121 gtatcgtcgg gtggtaccgg atgccggtaa cctgctggcg caacaggcaa ttgctgatgt
    36181 gttctgtgtc aacggcgaca gcgaatggcg cggcttaggc gtgattgaat cttctggcgt
    36241 gcacctgacg ccggattatc aacgattcga tgccgaagca catttccgcc cagcaccgca
    36301 gcaggtctgc gatgacccgc gcgcgcgttg tggtgaggta ttaacgggca aatgtaagcc
    36361 gcatcaatgc ccgctgtttg gtaacacctg taatcctcaa accgcatttg gtgcactgat
    36421 ggtctcctcc gaaggagcgt gcgccgcgtg gtatcagtat cgtcagcagg agaatgaagc
    36481 gtgaataata tccaactcgc ccacggcagc ggcggccagg cgatgcagca attaatcaac
    36541 agcctgttta tggaagcctt tgccaacccg tggctggcag agcaggaaga ccaggcacgt
    36601 cttgatctgg cgcagctggt agcagaaggc gaccgtctgg cgttctccac cgacagttac
    36661 gtcattgacc cgctgttctt tcctggcggt aatatcggca agctggcgat ttgcggcaca
    36721 gccaatgacg ttgcggtcag tggcgctatt ccgcgctatc tctcctgtgg ctttatcctc
    36781 gaagaaggat tgccgatgga gacactgaaa gccgtagtga ccagcatggc agaaaccgcg
    36841 cgcgcggcag gcattgccat cgttactggc gatactaaag tggtgcagcg cggcgcggca
    36901 gataaactgt ttatcaacac cgctggcatg ggcgcaattc cggcgaatat tcactggggc
    36961 gcacagacgc taaccgcagg cgatgtatta ctggttagcg gcacgctcgg ggaccacggg
    37021 gcgactatcc ttaacctgcg tgagcagctg gggctggatg gcgagctggt cagcgactgc
    37081 gcggtactga cgccgcttat tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt
    37141 gatgccaccc gtggtggtgt gaacgcggta gttcatgagt tcgcggcagc ctgcggttgt
    37201 ggtattgaaa tttcagaagt ggcactgccg gttaaacctg ccgtgcgcgg cgtttgcgaa
    37261 ctgctgggac tggacgccct taactttgcc aacgaaggca aactggtgat cgccgttgaa
    37321 cgcaacgcgg cagagcaagt gctggcagcg ttacattccc atccactggg gaaagacgcg
    37381 gcgctaattg gtgaagtggt ggaacgtaaa ggtgttcgtc ttgccggtct gtatggtgtg
    37441 aaacgaaccc tcgatttacc acacgccgaa cctcttccgc gtatatgcta ataaaattct
    37501 aaatctccta tagttagtca atgacctttt gcaccgcttt gcggtgcttt cctggaagaa
    37561 caaaatgtca tatacaccga tgagtgatct cggacagcaa gggttgttcg acatcactcg
    37621 gacactattg cagcagcccg atctggcctc gctgtgtgag gctctttcgc aactggtaaa
    37681 gcgttctgcg ctcgccgaca acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc
    37741 gtcttattac gcatcgcgtg aaaaagacac cccattaaat atgaagacga aactgttctg
    37801 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    37861 gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa atttggtcac
    37921 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    37981 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    38041 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    38101 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    38161 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    38221 attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    38281 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    38341 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    38401 gacgatttag ccccctatga acgcatgttg ttcgacacct agggcaacca gattcaaacc
    38461 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    38521 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    38581 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    38641 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    38701 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    38761 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    38821 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    38881 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    38941 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    39001 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    39061 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    39121 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccatag cgatctctat
    39181 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    39241 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    39301 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    39361 gaactggaaa acgtcattga gcgcgcggta ttgctaacac gcggtaacgt gctgcagctg
    39421 tcattgccag atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc
    39481 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacggcgtg
    39541 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    39601 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    39661 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    39721 tggtaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    39781 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    39841 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    39901 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    39961 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    40021 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    40081 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    40141 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    40201 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    40261 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    40321 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    40381 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    40441 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    40501 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    40561 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    40621 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    40681 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    40741 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    40801 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    40861 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    40921 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    40981 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    41041 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    41101 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    41161 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    41221 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    41281 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    41341 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    41401 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    41461 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    41521 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    41581 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    41641 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    41701 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    41761 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    41821 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    41881 ggcactggaa aaacagcttt atgaagagtt gttcgacctg ctgttgccgc atctggaagc
    41941 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg
    42001 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    42061 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    42121 gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt ccgaacatgg gcggtaaaag
    42181 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    42241 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    42301 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    42361 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    42421 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    42481 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    42541 cgtcgccaac gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag
    42601 cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt
    42661 accgaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    42721 gaacgccgcc gcgacgcaag tggatggtac gcagatgtct ttgctgtcag tgccagaaga
    42781 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    42841 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    42901 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    42961 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    43021 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    43081 ccgttaacat caactctctt tttaccctga aaccgactta cttatttcca caggcgataa
    43141 tattgatcgt gggccgaaga gtcttaacgt gctgcgcctg ctaaaccaac cctggtttac
    43201 gtcggttaaa ggcaaccacg aagcgatggc gttggatgca ttcgaaactg gcgatggaaa
    43261 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    43321 ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa tcattaatga
    43381 caacataaaa tatgccatc
//
LOCUS       DAZPLM010000011        43246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPLM010000011 DAZPLM010000000
VERSION     DAZPLM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.011"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            27..545
                     /locus_tag="ACHVC7_000525"
     CDS             27..545
                     /locus_tag="ACHVC7_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR2114663.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            561..1100
                     /locus_tag="ACHVC7_000526"
     CDS             561..1100
                     /locus_tag="ACHVC7_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR2114664.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1195..2772)
                     /gene="guaA"
                     /locus_tag="ACHVC7_000527"
     CDS             complement(1195..2772)
                     /gene="guaA"
                     /locus_tag="ACHVC7_000527"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR2114665.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2841..4307)
                     /gene="guaB"
                     /locus_tag="ACHVC7_000528"
     CDS             complement(2841..4307)
                     /gene="guaB"
                     /locus_tag="ACHVC7_000528"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR2114666.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4469..5839
                     /gene="xseA"
                     /locus_tag="ACHVC7_000529"
     CDS             4469..5839
                     /gene="xseA"
                     /locus_tag="ACHVC7_000529"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR2114667.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5836..6051)
                     /locus_tag="ACHVC7_000530"
     CDS             complement(5836..6051)
                     /locus_tag="ACHVC7_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR2114668.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6120..7592)
                     /gene="der"
                     /locus_tag="ACHVC7_000531"
     CDS             complement(6120..7592)
                     /gene="der"
                     /locus_tag="ACHVC7_000531"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR2114669.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7710..8888)
                     /gene="bamB"
                     /locus_tag="ACHVC7_000532"
     CDS             complement(7710..8888)
                     /gene="bamB"
                     /locus_tag="ACHVC7_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR2114670.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8899..9519)
                     /gene="yfgM"
                     /locus_tag="ACHVC7_000533"
     CDS             complement(8899..9519)
                     /gene="yfgM"
                     /locus_tag="ACHVC7_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR2114671.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9537..10811)
                     /gene="hisS"
                     /locus_tag="ACHVC7_000534"
     CDS             complement(9537..10811)
                     /gene="hisS"
                     /locus_tag="ACHVC7_000534"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR2114672.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10922..12040)
                     /gene="ispG"
                     /locus_tag="ACHVC7_000535"
                     /gene_synonym="gcpE"
     CDS             complement(10922..12040)
                     /gene="ispG"
                     /locus_tag="ACHVC7_000535"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR2114673.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12067..13080)
                     /gene="rodZ"
                     /locus_tag="ACHVC7_000536"
     CDS             complement(12067..13080)
                     /gene="rodZ"
                     /locus_tag="ACHVC7_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR2114674.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13365..14519)
                     /locus_tag="ACHVC7_000537"
     CDS             complement(13365..14519)
                     /locus_tag="ACHVC7_000537"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR2114675.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14669..15100)
                     /gene="ndk"
                     /locus_tag="ACHVC7_000538"
     CDS             complement(14669..15100)
                     /gene="ndk"
                     /locus_tag="ACHVC7_000538"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR2114676.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15249..17561)
                     /gene="pbpC"
                     /locus_tag="ACHVC7_000539"
     CDS             complement(15249..17561)
                     /gene="pbpC"
                     /locus_tag="ACHVC7_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR2114677.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17562..22523)
                     /gene="yfhM"
                     /locus_tag="ACHVC7_000540"
     CDS             complement(17562..22523)
                     /gene="yfhM"
                     /locus_tag="ACHVC7_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR2114678.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22730..23575
                     /gene="sseA"
                     /locus_tag="ACHVC7_000541"
     CDS             22730..23575
                     /gene="sseA"
                     /locus_tag="ACHVC7_000541"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR2114679.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23911..24036
                     /gene="timP"
                     /locus_tag="ACHVC7_000542"
     CDS             23911..24036
                     /gene="timP"
                     /locus_tag="ACHVC7_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR2114680.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24068..24844)
                     /gene="sseB"
                     /locus_tag="ACHVC7_000543"
     CDS             complement(24068..24844)
                     /gene="sseB"
                     /locus_tag="ACHVC7_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR2114681.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24986..26269)
                     /gene="pepB"
                     /locus_tag="ACHVC7_000544"
     CDS             complement(24986..26269)
                     /gene="pepB"
                     /locus_tag="ACHVC7_000544"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR2114682.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26447..26647)
                     /gene="iscX"
                     /locus_tag="ACHVC7_000545"
     CDS             complement(26447..26647)
                     /gene="iscX"
                     /locus_tag="ACHVC7_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR2114683.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26659..26994)
                     /gene="fdx"
                     /locus_tag="ACHVC7_000546"
     CDS             complement(26659..26994)
                     /gene="fdx"
                     /locus_tag="ACHVC7_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR2114684.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26996..28846)
                     /gene="hscA"
                     /locus_tag="ACHVC7_000547"
     CDS             complement(26996..28846)
                     /gene="hscA"
                     /locus_tag="ACHVC7_000547"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR2114685.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28863..29378)
                     /gene="hscB"
                     /locus_tag="ACHVC7_000548"
     CDS             complement(28863..29378)
                     /gene="hscB"
                     /locus_tag="ACHVC7_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR2114686.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29474..29797)
                     /gene="iscA"
                     /locus_tag="ACHVC7_000549"
     CDS             complement(29474..29797)
                     /gene="iscA"
                     /locus_tag="ACHVC7_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR2114687.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29814..30200)
                     /gene="iscU"
                     /locus_tag="ACHVC7_000550"
     CDS             complement(29814..30200)
                     /gene="iscU"
                     /locus_tag="ACHVC7_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR2114688.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30228..31442)
                     /gene="iscS"
                     /locus_tag="ACHVC7_000551"
     CDS             complement(30228..31442)
                     /gene="iscS"
                     /locus_tag="ACHVC7_000551"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR2114689.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31683..32171)
                     /gene="iscR"
                     /locus_tag="ACHVC7_000552"
     CDS             complement(31683..32171)
                     /gene="iscR"
                     /locus_tag="ACHVC7_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR2114690.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32472..33209)
                     /gene="trmJ"
                     /locus_tag="ACHVC7_000553"
     CDS             complement(32472..33209)
                     /gene="trmJ"
                     /locus_tag="ACHVC7_000553"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR2114691.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33328..34131
                     /gene="suhB"
                     /locus_tag="ACHVC7_000554"
     CDS             33328..34131
                     /gene="suhB"
                     /locus_tag="ACHVC7_000554"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR2114692.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34276..35130
                     /locus_tag="ACHVC7_000555"
     CDS             34276..35130
                     /locus_tag="ACHVC7_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2114693.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35321..36601
                     /gene="csiE"
                     /locus_tag="ACHVC7_000556"
     CDS             35321..36601
                     /gene="csiE"
                     /locus_tag="ACHVC7_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR2114694.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36593..37732)
                     /locus_tag="ACHVC7_000557"
     CDS             complement(36593..37732)
                     /locus_tag="ACHVC7_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR2114695.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37892..38782)
                     /gene="hcaR"
                     /locus_tag="ACHVC7_000558"
     CDS             complement(37892..38782)
                     /gene="hcaR"
                     /locus_tag="ACHVC7_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR2114696.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38918..40279
                     /gene="hcaE"
                     /locus_tag="ACHVC7_000559"
     CDS             38918..40279
                     /gene="hcaE"
                     /locus_tag="ACHVC7_000559"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR2114697.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40276..40794
                     /gene="hcaF"
                     /locus_tag="ACHVC7_000560"
     CDS             40276..40794
                     /gene="hcaF"
                     /locus_tag="ACHVC7_000560"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR2114698.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40794..41114
                     /locus_tag="ACHVC7_000561"
     CDS             40794..41114
                     /locus_tag="ACHVC7_000561"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR2114699.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41111..41923
                     /gene="hcaB"
                     /locus_tag="ACHVC7_000562"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41111..41923
                     /gene="hcaB"
                     /locus_tag="ACHVC7_000562"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR2114700.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41933..43135
                     /gene="hcaD"
                     /locus_tag="ACHVC7_000563"
     CDS             41933..43135
                     /gene="hcaD"
                     /locus_tag="ACHVC7_000563"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR2114701.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10259 a        11947 c        10860 g        10180 t
ORIGIN      
        1 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
       61 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
      121 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
      181 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
      241 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
      301 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
      361 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca
      421 aagtgtatac ctccacccag gtgggtaaag agtgccagtt tacgacaggt ttagccgttg
      481 ttattaccac aacgtataac gaaacgcgta ttcagccaaa tactaaatgt cctgaaaaga
      541 gctaataatc aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa
      601 gctatccggc tatcgcctca tcattgcagg atcaactctc tgctgtcgca gaagcggaac
      661 agcaaggtaa aaatgaagag caaaggcagc atgacgaatg ggtcgcggag cgcaacaggg
      721 aaatccagca agagaagcaa cgtcgcgcaa atgcccaggc cgccgctaac aaaagagcgg
      781 caacggcagc ggcaaataag aaagctcgtc aggataaact ggacgccgaa gccactgcgg
      841 acaaaaaacg cgatcaaagt tatgaagatg agctacgcag cttagagatt cagaaacaaa
      901 aactgacgct ggcgaaagaa gaagcccgcg ttaagcgaga aaacgaattt atcgatcagg
      961 aactgaagca caaagctgcg caaaccgatg tggtgcaatc tgaagctgac gccaacagaa
     1021 atatgactga aggcggtcgc gatctgatga aaagcgtggg caaagcagaa gagaacaaat
     1081 cggacagctg gtttaattaa tcgatgttag taacttcaag cctatgattc ttgaagataa
     1141 aaaaccctct gtagtaacag agggttttgt tcattcatag tgcagggatt aaaatcattc
     1201 ccactcaatg gtagctggcg gcttgccgct gatgtcatac accacgcggg aaataccgtt
     1261 cacttcattg ataatgcggt tggaaacgcg accgaggaaa tcgtacggca gacgcgccca
     1321 gtgtgcggtc ataaagtcga tggtttcgac agcacgcaga gagacaaccc agtcatactt
     1381 acgaccatcg cccattacgc caacggaacg taccggcagg aacacagtga acgcctggct
     1441 gactttgtcg tacaggtccg ctttacgcag ttcttcaatg aagatggcgt cagcacggcg
     1501 cagcaggtca cagtactctt tcttcacttc acccagcaca cgaacgccaa ggcctggtcc
     1561 cgggaatggg tgacggtaca acatgtcgta cggcaggccc agctccagac caatcttacg
     1621 cacttcgtct ttgaacagct ctttcagcgg ttcaaccagg cccatcttca tctctttcgg
     1681 caggccgccc acgttgtggt gagatttgat gacgtgtgct ttaccggttg cagacgccgc
     1741 agattcgata acgtcagggt agatggtgcc ctgcgccagc cacttcacgt cttccagttt
     1801 cagcgcttct tcatcgaata cttcaacgaa aacgcgaccg atgattttac gttttgcttc
     1861 cggatcgttt tcgccagcca gcgctgacag gaagcgatct tctgccggaa cgtgaacaat
     1921 gttcagacca aagtgatcgc caaacatatc cagaacctgc tctgcttcgt tgagacgcag
     1981 caggccgttg tcgacgaata cgcaagtcag gtttttaccg atagcgcggt gcagtagcat
     2041 tgcggttacg gaggaatcca cgccaccaga gaggccgagg atgactttat cgtcgcctac
     2101 ctgctcacgg atgcgggcta cagcatcgtc gataattttc gctggcgtcc acagggcttc
     2161 acactggcag atatcacgca caaaacgctc cagcatgcgc ataccctggc gggtgtgagt
     2221 cacttccggg tggaactgta cgccatagaa gcgtttttct tcgttagcca taatggcaaa
     2281 cgggcagctt tcggtgctgg ctacggtgac gaagtcggac gggatagcgg taactttatc
     2341 gccgtggctc atccagacat cgagcagcgg tttaccgtct gcggtcagcg catcttcgat
     2401 accgcgaacc agtgcactgt cgtttacgac ttcaacctgc gcgtagccaa attcacgttc
     2461 gttagaggct tcaacgtgac cgcccaactg cattgccatg gtctgcatgc catagcaaac
     2521 gccgaatacc ggtacgcctg cttcaaagac atactgcggc gcacgcgggc tgttttcttc
     2581 agtagtactt tccgggccgc cggaaagaat aatgccgctt ggattgaagt cacgaatttg
     2641 tgcttctgtc acatcccacg cccacagttc gcagtaaaca cccagctcac gcacgcggcg
     2701 cgcaaccagt tgagtgtact gagaaccgaa gtccagaatg aggatgcgat gcttatgaat
     2761 gttttccgtc attgacgctt attccgaggc aagtgaaaca gataatataa atcgcccgac
     2821 atgaagtcgg gcgaagagaa tcaggagccc agacggtagt tcggggactc tttagtaatg
     2881 gtcacgtcgt gaacgtggct ttcctgaatg cccgcaccgc tgatacgtac aaactccgct
     2941 ttagtacgca gttcgtcgat agtaccacag ccggtcagac ccatacagga gcgcaggccg
     3001 cccatctgct ggtgaatgat ctctttcagg cgacctttat aagctacgcg accttcgata
     3061 ccttccggca ccagtttgtc ggcagcgtta tcgctctgga aataacggtc agaggaacct
     3121 ttggacatcg cgcccaggga acccatacca cggtaagatt tgtaagaacg gccctggtag
     3181 agttcgattt cacccggaga ttcttcagta cccgccagca tggaacctac catcaccgcg
     3241 cttgcgccag cggcgatagc tttggcgatg tcgccggaga agcgaatacc accatctgcg
     3301 ataaccggaa taccggtgcc ttccagggct tcaactgcgt cagcaacggc agtgatctgc
     3361 ggaacaccta cgccagtaac gatacgagtc gtacagatag aaccagggcc gataccgact
     3421 ttaaccgcgc tgcaaccagc ttctgccaaa gcgcgtgcac ctgcagctgt tgccacgttg
     3481 ccgccgatga tttgcagatc cggatattta gcacgggttt cacggatacg ctgcaaaacg
     3541 ccttctgagt gaccgtggga ggagtcgatc agcagaacgt caacgccagc ggcaaccagc
     3601 gcgtcaacac gctcttcgtt acccgcacct gcgccaactg cagcaccaac acgcagacgg
     3661 ccttgctcgt ctttacaggc gttcggttta cgttccgctt tctggaagtc tttcacggtg
     3721 atcatgccga tcaggtggaa ttcgtcatca accaccagcg ctttttcaac gcgtttttcg
     3781 tgcatttttg ccagcaccac ttcacgggct tcaccttcac gcacggtgac cagacgctct
     3841 ttcggcgtca tgtaaacgct aaccggctgg ttcaggtcgg taacaaaacg cacgtcacga
     3901 ccggtgataa tacccaccag ttcgttttct tcggtaacga ccggatagcc cgcaaaaccg
     3961 ttacgctcgg tcagttcttt cacttcgcgc agcgtcgtgg ttggcagaac agtctgcgga
     4021 tcagtcacca caccagattc gtgttttttc acacggcgaa cttcttctgc ctggcgttca
     4081 atggacatgt ttttgtggat aaagccgata ccgccttcct gagccagagc aatagccagg
     4141 cgcgcttccg ttacggtatc cattgctgcg gaaagcatag ggatattcag acgaatagtt
     4201 ttcgtcagct gggtgctgag gtcagcagta ttcggcagaa cggtagaatg agcaggaacg
     4261 aggagaacgt cgtcaaacgt cagagcttct ttagcgatac gtagcatggg caatatctcg
     4321 accagagtgg ttaataaata ttgccgcggc attatacaga gcgtaaccga ttgcatctac
     4381 ccctttttgc aaaaaatgct tgctatcccc gaagggcggg ttactatcga ctgaataacc
     4441 tgctgattta gaatttgatc tcgctcacat gttaccttct caatcccctg caatttttac
     4501 cgttagtcgc ctgaatcaaa cggttcgtct gctgcttgag catgagatgg gacaggtttg
     4561 gatcagcggc gaaatctcta atttcacaca accggcttcc ggtcactggt actttacact
     4621 caaagacgac accgcccagg tacgctgcgc gatgttccgc aacagcaacc gccgggtgac
     4681 cttccgtcca cagcatggac aacaagtttt agttcgcgcc aatattacgc tctacgagcc
     4741 gcgcggtgac taccagataa tcgttgagag tatgcagccg gccggtgaag ggctgctgca
     4801 acagaagtac gaacagctca aagcgaagtt gcaggctgaa ggtttgttcg atcagcaata
     4861 caaaaaacca cttccctccc ctgcgcattg cgttggtgtg atcacctcaa aaaccggtgc
     4921 tgcgctacat gatattttgc atgtgttaaa acgtcgcgat ccttctctac cggtgatcat
     4981 ctaccccacc gccgttcagg gcgatgacgc accggggcaa attgttcgcg ccattgaact
     5041 ggcgaatcag cgcaatgagt gcgacgtgtt gatcgttggg cgcggcggcg gttcgctgga
     5101 agatttatgg agttttaacg acgaacgcgt agcgcgggcg atttttgcca gccgcattcc
     5161 ggtcgtcagc gccgtcgggc atgagacgga tgtgaccatt gccgattttg ttgccgatct
     5221 gcgtgcgcca acgccgtctg ccgccgctga agtagtgagc cgtaatcagc aagagttact
     5281 gcgccaggtg caatcgaccc gtcaacggct ggagatggcg atggattatt atctcgccaa
     5341 ccgcacgcgt cgctttacgc agatccatca ccgattacag caacagcatc cgcagctccg
     5401 gctggcacgc cagcaaacca tgcttgagcg cctgcaaaag cggatgagct ttgcgctgga
     5461 aaatcaactt aagcgtaccg ggcaacagca gcagcggtta acacagcggc tgaatcagca
     5521 aaatccacag ccgaagattc atcgcgcgca aacgcgcatt cagcaactgg aatatcgttt
     5581 agcagaaacc ctgcgcgtac agcttagcgc cacgcgtgaa cgtttcggta atgcagtaac
     5641 gcacctcgaa gccgtaagcc cactgtcaac gctggcgcgt ggatacagcg ttactactgc
     5701 tactgacggc aatgtactga aaaaagtgaa gcaagttaaa gcgggtgaaa tgctaaccac
     5761 acgtctggaa gacggctgga tagaaagtga agttaaaaac atccagccag taaaaaaatc
     5821 gcgtaaaaag gtgcattaag ccagcacgaa ttcaacgcgt tttttcgata tcaagccatg
     5881 cccgtgctga cagaagtaat ctactgcacc gcaggctttt aacacctgca acggttgatg
     5941 gcagtctgga caaagagctt tcatttcaat aatttccccg caggatgggc aacgcgcatg
     6001 gccgttatcc tgatcaagca catgctgaca ttgtggacaa tgcagttcca tatgaattcc
     6061 tccggtagcc atccctctac attcatagag ggaatggcag ataaaatact tacggataat
     6121 tatttatttt tcttgatgtg cttcatcaga cgcttacgtt tacgcatctg ggttggcgtc
     6181 agggtgttac gcttgttcgc atacgggttt tccccttctt tgaactgaat acgaatcggc
     6241 gatcccatta cgtccagcga tttgcggaag tagttcatca agtagcgctt gtaggaatca
     6301 ggcaggtctt tcacctgatt accgtgaatc accacaatcg gcgggttata accaccagcg
     6361 tgggcatatt tcagcttcac acgacgaccg cgtaccagcg gcggttggtg atcttcaaca
     6421 gccatcgtca tgatgcgcgt cagcatagag gtccccacac gacgggtgga gctgtcatac
     6481 gcttcacgta ctgattcaaa caagttacca acaccactgc cgtgcaaggc agagataaag
     6541 tgcacacgag caaaatcgat aaagcccaga cggaagtcca gcgtttcttt cacctgctct
     6601 ttcacttcct ggctcaggcc atcccactta ttcaccacaa tgacaagtga gcgcccacta
     6661 ttgagaataa agcccagcag cgagagatcc tgatcggaaa taccttcgcg cgcatcaatc
     6721 actaacatca ccacgttggt atcttcaatg gcctgcaacg ttttgattac ggagaatttc
     6781 tctacagcat cggtgatttt gccgcgttta cgtacgccag cggtgtcaat gagcacatac
     6841 tcacgtccat cgcgttccat tggaatgtag atactgtcac gcgtcgtgcc aggcatgtca
     6901 taaacaacaa cgcgctcttc accaagaata cggttagtga gtgtagactt acctacgttc
     6961 ggacgaccca caatcgccag tttgatcggc agactttgcg ggtcgaagtc gtcttcctct
     7021 tcttcttcgc cgttctcttc cgcttcaaat tgcgcccagt attcagcgtc ttcgtcgact
     7081 tcctcttgcg gtgcgagatc ttccatccac ggcagcagca cacgctccag cagacttaat
     7141 acgccacgac cgtgagatgc ggcgatcggg tagatttcac ctaaaccaag cgagtagaaa
     7201 tcaaccactg cctgatcggg atccagaccg tcagttttgt ttgccaccag gaaggtcggt
     7261 ttttcacgag agcgcagatg tttggcaatc gcttcatctg ccggcatcaa gcccgcgcgc
     7321 gcatccacca taaacagtac gacgtccgct tcttcaatcg ccagcagcga ctgttccgcc
     7381 atgcgggttt ctacaccgtc ttctgtgcca tcaatcccgc cggtatcaat acagataaac
     7441 tcacggcctt caatttccgc acgaccgtac ttacggtcac gagtcagacc cgggaaatcc
     7501 gcaaccagcg catctcgggt gcgagttaga cggttaaata acgtggattt tcctacgtta
     7561 gggcgcccga caagcgcgac cacaggtacc atgtttaaag cctcattttt ataaatcatc
     7621 agacaacgca cgctatattc gcgtcgttgt taaaaacagg aaaacggccc ctgtccagga
     7681 gccgttttca aagtgaacga cagagacgat taacgtgtaa tagagtacac ggttccgtct
     7741 tttgcctgga tcagcagttt gccgtcagcg gcaaccggtt cagtctggaa accggaacta
     7801 tcaacttttt gctgggcaac gaaacgacca tcttcgacgt taatccagtg cagataacct
     7861 tcactgtcac ctaccaccag gttgccatta tacagcaccg gcgaagtcag caggcgatgc
     7921 agcagatcgc tttgtgtcca cagcgtaacg ccgccatcaa tggtcaacgc catcacccgg
     7981 tcattttgat cgaccagata gatgcgattg ccgtcgacga tgaaatcatt caccgaaccc
     8041 agttcgcgtt tccacataat ctgaccactg cgcagatcaa gcgccgtcag gttaccatta
     8101 taggccagag cgaaaacaac gccgttaaca acgacgggag tcgtgtcaac atcgctcaga
     8161 cggtcaattt cggtagaacc ggtcgcctgg gaaatacgct gctgccaaat catctggccc
     8221 tgttccatca gcactgcgct gacgcgacca ttatcgcccc ccacgacggc cgcaccaaaa
     8281 gccgttgccg gcgcagactc gccacgcaaa gagagcgaag gcatatcgag gttaactgtc
     8341 catttgacag cgccgtcagc ttcgttcagc gcttgtaact gaccgttact agtgtggatt
     8401 aacaccagac cgtcgctgac caccgggcga gaaagtgctt cacccgcgac tttagtttgc
     8461 catgccacag taccatcgct ggtattcagc gcgtaaacct gcgccttttc gctgccaatg
     8521 tagacatgcc caccagacac ggtcacaccg ccagaaagta atgcaggctc tttagagaac
     8581 cagccatctt tctcggccag gctgacagac cagatttctt tgccatcatc cgcattcagc
     8641 gcttttacca aaccagcgcg gtccgctgca tagacaacgt tgtccgccag tgccggatga
     8701 agattggaat agaagttgcc aatgccgcta ccaacggaag tgctccacgc cgtggtcggc
     8761 gtaaactggt tttcaacggt tggcaatggg gacatcttta ccacatcttc ttcgctgtta
     8821 aacagcgaac agccgcttaa aagggtaacg gaaagcagtc ctggcagcag taatttacgc
     8881 aattgcatcg ggtccctctc agatggacaa attattaatt ttcatctgca tcatttcgct
     8941 cagtgccgga gtaacatcgc ttttcacgcc tgcttcccat gcactacgcg caccttgctt
     9001 atcacctttg ctcaacaatg ctttaccacg caggtcggca acaatggcag cccacccttc
     9061 acctttgatg gtatcaaggg ttttcagcgc ggcatcagcc tgcttgagct gtacctgaac
     9121 gcgagcaaga cgcagattta tcacggcttt gagattttca tcactcgtgt ctgccagccc
     9181 ctgttgtaac tgggcggcag ctttctctag ttcatttttg tcaacaaatt gctgcgcaag
     9241 ttccaaagaa gccagcgcac cataagtatt tttattttca gcagcaaatt tttccgccgc
     9301 cgggatgcta tccggtttgc cttcgctcac tgcggtaacc gcattttgat aggcaagaga
     9361 agcggagcgt gcagaatcaa cctgatggct gttccagtag cgccagccaa tcagtgcgcc
     9421 aacgcccaaa atcaccccaa cagccagtgc tttgccattt tcagcaaaaa agcgtttaac
     9481 cgcttctacc tggtcgtttt cgttctcgta aatttccacg ctgtccttct ccttccttaa
     9541 cccagtaacg tgcgcaaatg cgcggctacg ctatcctgcg caactgccgt ttgctcacca
     9601 gagcgcaaat ccttcactac tgctgtgccg ttagccactt cagactcacc cagcaccaca
     9661 gcaacgcggg caccccattt atcagcacgg gcaaactgtt tcttaaagtt gccgccgccg
     9721 tggttggtca tcaatttcac gcccggtaat tcatcacgca gacgctcagc taatgccata
     9781 gccgcagatt gtgtatcagc acctgaagcc accaggtata tatcgacaac aggatcggct
     9841 ttaaattccg gattaacggc ctgtactaac aatacaagac gttcgaggcc catcgcaaaa
     9901 ccgactgccg gtgttgcacg accgcccagt tgttccacca gaccgtcata acgaccgcct
     9961 gcacacacgg tgccctggga gccgaggctg ttagtcaccc actcgaaaac ggtacggtta
    10021 tagtaatcca gaccacgcac cagacgctgg tttacggtgt aagcgatccc cgcgctctca
    10081 agcagtttgc acagaccggc aaaatgctca cgagattcct cgtccagata atcacctaat
    10141 gccggagcgt cgttgagaag cgcctgcact tccggatttt ttgaatccag cacgcgcagc
    10201 gggttagtgt acatgcggcg tttgcagtct tcgtccagct tttctttatg ctgctcaagg
    10261 aatgccacca gcgcatcgcg gtaattggcg cgtgcttcca gcgaaccaat agagttcagc
    10321 tcaagagtta cgtgttcgaa gatacccagc gcacgccacc agcgggcagt gagcataatc
    10381 agttcagcgt cgatatccgg accttgcaga ccgaaaactt cgcagcccaa ctgatggaac
    10441 tgacgataac gccctttctg cggacgctcg tgacggaaca tcggcccgat ataccacaga
    10501 cgctgttcct gattgtacag aagaccatgc tcgatgccgg cgcgtacaca gcccgccgtc
    10561 ccttcagggc gcagagtcag gctgtcgcca ttgcgatcct caaaggtgta catctctttt
    10621 tcaaccacgt cggtgacttc accaatcgca cgtttgaata gcggggtctg ctctacaatc
    10681 ggcaagcgga tttcactgta accgtagctg ccgagcacgt ttttcagtgt gccttcaatg
    10741 cgctgccaga tggccgtttc gccaggcagg taatcgttca tgccgcgaat ggcttgaatg
    10801 ttttttgcca cgtttattct ctttctgaat ataaaaatga accctcaacg cttccctcaa
    10861 tgtttcggga gccatgcggg ttcaatcata cacgggaagc gaggcgcttc ccatcacgtt
    10921 attatttttc aacctgctga acgtcaattc gacgcgcttc gtccagctga ctggctttcg
    10981 cacgaatgcg tgcttccagc tggtcgatca tatcgttgtt gtccagacgg tctttgcgca
    11041 cgccatcttc atagaggccg cttttcttgt tgccgccggt gacgccgagt gtagaaacca
    11101 gcgcctcacc tgggccattc accacgcagc cgataatcga aacgtccatc ggagtgatga
    11161 tatcttccag gcgttgctcc agcgcgttaa ccgtaccgat aacatcaaat tcctgacgcg
    11221 aacaggtcgg gcaggcgatg aagttaatcc ctcgcgaacg gatacgcagc gatttcaaaa
    11281 tatcgaaacc gactttgatc tcttcgaccg gatcggccgc cagcgatacg cgcagcgtgt
    11341 cgccgatgcc ttccgacagc agcagaccta aaccaatggc ggattttact gccccgctgc
    11401 gtgcaccacc ggcttcggtg atccccagat gcagcggctg atcgatctgt tttgccagca
    11461 aacgataaga ctcaacagcg aggaagacgt cagacgcttt cacgctgact ttgaactgat
    11521 cgaagttcag gcgatcgaga tgatcaacat gacgcatggc ggattccagc aacgcctgcg
    11581 gcgtcggttc gccatacttt tcttgcagat ctttttccag cgatccggcg ttaacgccaa
    11641 tacggatcgg aatgttttta tcgcgcgcac agtcaaccac catgcgaata cgctcttcat
    11701 taccgatatt gccagggtta atacgcagac aatcgacgcc gtattccgct actttcagcg
    11761 caatgcgata gtcgaagtgg atgtcagcca ccagcggcac gttaacccgc tgtttgatga
    11821 gtttgaacgc ttctgccgcg tccatcgtcg gtacggagac acggacgata tcagcgccaa
    11881 cgcgttccag cgccttgatt tgattgaccg ttgcttcgac atctgtcgta cgcgtattgg
    11941 tcatggactg tacggcgatg ggagcaccat cgccaatcgg cacattccca acgtaaatac
    12001 gtgttgattt tctacgttga attggagcct ggttatgcat gaaaaatctc ccgcgttacc
    12061 cgtctgttac tgcgccggtg attgttcggc attgagggtc agacgcgcaa cctggttagt
    12121 tctgataaaa cgactcagat cgacaggttt cccttgatac tggatctgta ctgcggctgg
    12181 cgcaccaatt ttcagtttat acggtgcctg gccggttaaa ttcaaattac cgtctttacg
    12241 ctgcatcccg ctaaacaatt ttttaccggt agcatcagtg acctccagcc agcaatcggc
    12301 agtaaagttc atcaccagcg cattcggatc agccgccggc gtggtcacgc cagcctgatc
    12361 ggttggcaac ggcgcagcac catctggcgt tgttgctgcc gttggtaccg gggtcgcggc
    12421 ggtatcaaca tttgcctgcg aaggcgaaac aaccgcattc tgttgcggat caacagctgg
    12481 tgctggcgca gttacggcag gtgtttgcgt gttggttgcg gtagtatcca cagacgctgg
    12541 cggcgtggac gttgtagccg ggtctgtagt tgtcgacgta tttaacggga cactctgccc
    12601 ctgctcgcta ttactgctca gttctgccga agattgatcg gccatagtgg tgatctcttc
    12661 ctgctgagct ttgtggtctt gccaccacca ggcaccgctc aggccgataa ccacaaacaa
    12721 caccagccag gtgaaggtca tcagccagcc atcacgtttt ttgcggcgtt taccgaggga
    12781 aaaactctgc attggcgcaa cttttgcagc ccgtagtgga gcctgctttt ccagccctgg
    12841 cagcagttct tcttctggaa tatgtaccag acgcgcataa gagcggatat atccgcgcag
    12901 gaatgttgaa gcaagatcgg cgggtgcctt atcttcttca atgtcgcgta ccgtggaaac
    12961 cttcaggcaa agtcgctcgg caacggcctg ctgactaagt cctagttgtt cgcgagcatt
    13021 acgcaggcga gcgccggtag taagtgcttc attttggtcg tgcgtggctt cagtattcat
    13081 tcgctgcagg tacgtttaaa tgagaattga gatgccggtg aaccatcatg ctcacccaca
    13141 ccgcgaaaca tccgttaagt taaccgttat catacagtat aagaccgtct gtgtgctcgt
    13201 gacaaagccc acattcacta aggctaactt actgttgcat cgttacatac tgccttaaag
    13261 tcagcaaaaa cgcaccgtta atattgacca gacaattgca acttaatact tcattcataa
    13321 aacattacgc cacggtacat aaagtaaccg tggcgtaatg gctatcagac cgctttaatg
    13381 tcgatggctt caccctgcat ccgtttacgc agggtacgtt tcgtacggtc gataacatcg
    13441 cccgccaact gaccacaggc agcatcaata tcatcaccac gagttttacg cacaatagtg
    13501 gtgaaaccgt agctcatcag cacttttgag aaacggtcga tacggctgtt cgagctgcgt
    13561 ccatacggcg cacccgggaa cgggttccac gggatcaggt tgatcttaca cggcgtatct
    13621 ttcagcagtt ccgccagttg gtgcgcgtgt tcagtgccgt cgttaacgtg gtcaagcatc
    13681 acgtattcaa tagtgactcg gccctgattg gcgttggatt tctccagata acggcgcacc
    13741 gcagcaagga acgtttcgat attgtacttt ttgttgatcg gcacaatttc gtcacgaatt
    13801 tcgtcgttcg gcgcgtgcag ggaaattgcc agtgcaacgt cgatcatatc gcccagttta
    13861 tccagcgccg gaactacacc ggaagtagaa agcgtgacgc gacgtttaga caggccaaaa
    13921 ccgaaatcat caagcatgat ttccatcgcc ggaacgacgt tgttcaggtt gagcagcggc
    13981 tcgcccatgc ccatcatcac tacgttagtg attggccgct gaccggtgac ttttgctgcg
    14041 ccgacgattt tcgccgcccg ccacacctgg ccgataattt ccgacacccg caggttgcgg
    14101 ttaaagccct gctgggcggt ggaacagaat ttacactcca gcgcacaccc cacctgcgaa
    14161 gagacgcaga gcgtggcacg gtcgtcttcc gggatataca ccgtttcgac gcgctgatcg
    14221 ccaacggcga tcgcccattt aatggtgccg tcggatgaac gctgttcttc aaccacttcc
    14281 ggtgcgcgga tctccgccac ctctttcagt ttgccgcgca acactttgtt gatgtcggtc
    14341 atctcatcaa agttgtcgca gcaatagtga tacatccact tcatcacctg atcggcgcgg
    14401 aagggttttt cacctaaatc tttaaaaaac tcccgcatct gctgacggtt gagatccagc
    14461 aggttgattt ttccatcttt cgtggtgacg ttttcaggtg tgactaattg ttcagacata
    14521 tgctattccg gcctcgttat tacacgttat ggcccctgga gggttgaaaa aagaaacgcc
    14581 ccggtgagcg gctgctcgtc cgggggcgct gcattgtaca aattctggcg cacggatgcc
    14641 acgtttgcac gcggcattta cgaaattatt aacgggtgcg cgggcacact tcgccttcgc
    14701 cgaagaaata agcgatttcg cgagcggcag attcgacgga atcagaaccg tgggtaccgt
    14761 tttcggtcag gctgtcagcg taatcagcgc gcagagtacc ggccagtgcg tttgccggat
    14821 tggtcgcgcc cagcagatcg cggtgacgct gaacggcgtt ttcaccttcc agcacggaaa
    14881 ccacgatcgg gccagaggtc atgaattcaa ccagaccatc aaagaacggt tttccatcgt
    14941 gttcagcata aaagccacga gcctgttcaa cggtcaggtg cagcattttg gtgccgacaa
    15001 ttttgaaccc tgcagcttca aagcgcgcaa agatattacc aatgacgttt tttgctaccg
    15061 cgttcggttt gatgatggaa aaagtacgtt caatagccat ttttacctct gtaaattgtt
    15121 ctgttgttgt ctgtaccagc gtacgagatg gcgcggatta taatgagcaa cagggccgtt
    15181 gactattgat gaaggtaaca tttttttaaa ataaaatgag ttttagcaac aatcagggac
    15241 aaaacagact attgcatgac aaatttcact gtcgcgattt gccccacgtc atccatcacc
    15301 agcaattgat aatcaccttt atccgtcaaa tgcagggtca cgttgcgccc gcgttcagtt
    15361 aacggttcgc cattcagaaa ccaccagcgt tcacctgccc ctccacttga ttgcaacggc
    15421 aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatcg cgccatcgcg aacgcctgtc
    15481 agctgcagag ggagctgggc atcgtgaccg tatggcgggc aaattgtcga ggccggtggt
    15541 aagcgcacag cgcgacgttc tgatgcgggc agccagggtt ccagcggtag cggccagaca
    15601 tttaccattt cttgcttcgc ctgcgggcag tcagcggcga cgcgctgccc tttgtcatcc
    15661 agccagatgg ggaagcgaat gccattaatc ccttcctgct ctgacagtaa cagcgttggc
    15721 ggctggctgc catccagcag ccaggttgcc aggcggcggc gacagttacc gtcaccttcc
    15781 ggcaaagact gtccgcccgg ccagcagata acgccacgag tgactgagtt cgggcgcggg
    15841 tcttccggca gattcgcact gcgcgacagt aagatattat tgacctgatt caacaatggt
    15901 acggcactgg caaagccaaa ctgaccaaca acgggcgtgc cgtcaggtct gccagtccag
    15961 atcccaatga catagcgagc gttaacccca atcgcccagg cgtcacgata gccatagctg
    16021 gtgcccgttt tccatgccag tggggcgacg cgcggcaagg cactatccgg caagggttgc
    16081 gcttcatcag ccataatccg gcgaatgatc cacgccgccc ccgacgacat taaaggccgt
    16141 tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg ccttgccgtg gcgagcaaac
    16201 gcggtatacg ctgccgccat atcttccagt tttgcaccag cgccgccgag aatgagtgaa
    16261 agattcggcg cagcgccgtt gggcaaatat aacggcaatc caacattgcg taactttgcc
    16321 gcaaaccgtt tcggtccata ggcttccagc acctgcacag caggtaagtt cagcgagcgc
    16381 accagcgcct cgctcatgct gatcgggcca tgaaaaccgc tatcaaagtt acctggtcga
    16441 taatcaccgg tgcgccgggg gacgtcttgc agcagtgatg ccgggtggat caagccttca
    16501 tccagcgcca gaccataaac aaacggtttg agcaccgatc ctggcgatcg gatcgcattg
    16561 accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgatcc cacccagccg
    16621 cgaacacgca tatcggtatg atcaaccacg atcatcgcca gtgagctgcg cggtggcaac
    16681 cgccctttcc agttttgcgc cagttcttcc agacgtcgtt gaagaccggc atccagcgta
    16741 gtaacgattt tgtcgctttt gcttttaccg agcatcatgc gcgaaaacag cggtgccagt
    16801 tgcggcattt gtcggggtgc cagccagatg ggttcttccc ttgactcttt tacctgctca
    16861 cgggaccaca caccttgcgc agccatccgt tcgagcactt tattacgcgc ggcttcggca
    16921 cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt gcggcaaaac cgccagcatt
    16981 gccgcctcgg aatagcttaa attcgcaggc gactttccga gataagccca acttgccgca
    17041 ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa gatacaaggt cagaatttca
    17101 cgcttagaca gatgccattc cagttgcaac gcgcgccaga gctggcgaat tttgccgcca
    17161 aatgttttgg ggtgaggatc aagcagacga gcaacctgca tagtgagcgt gctgccaccg
    17221 gaaataaccc gtcccgaagt gagatcttgc catgctgcgc gcgccaccga gaatggattc
    17281 acccccggat gcttccagaa ccagcgatct tcataattga tcagcgcttc aaggtaacgt
    17341 ggagaaacat cttcgattgt taccggataa cgccagatgc cgtcagcatc ggcgaagcgc
    17401 cagagcggcg taccatcctg cgccacgacc actcgtgcgg gattgacttc atgcagcggt
    17461 agaggccaga gtttatctgc tccccacgct gcaagaaaaa ggagaaatgg cgcagctgcc
    17521 agcgttatcc agcagccgcg tttggttaac aagcgaggca tttacggtcg gacaatcagc
    17581 agatcttcag ccgcgccggt cgcccgccat tggggaacat acattgattc caccatcggt
    17641 tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca gatacaccag cgttaccggt
    17701 tggtattcat caacggcaac cgccgccaca aagcgatcgt cacggaactc aatgtgctta
    17761 atactcgcct gctgcatctg gttcagtaag ttctgcactt cgccaccact ttgctccagg
    17821 ctggcgctac cgttcgccag attctggttt tccagttcca gacccacagg cagcagatcc
    17881 acgactaacg catccggcac gctgttactg gcttttacct gcaaccacac cagcaccaga
    17941 tcgccgctac gtaacgagtc cagcgatttg ctcttaccat cagtaccaag aatatgacgc
    18001 tcgatttgca gcacattgtt cgccggtaaa ggtgcggatt gcggataacc gctggcatcc
    18061 atacgcaacc ataacggctg atcgccactg ttgctcacct gcaaggtgac aagttgatcg
    18121 ctattcagat tgctgttttg cgctttctcg cctgtcagcg gctcagctga gaaagaggtt
    18181 tgcgcctgcc atttacccgg taaatcctga atcgtacggg cagccaggaa caacgcgtta
    18241 ctttcctgcg tcgatagcca gcgttcacca aacgcctgct gcgaaagtgt gttcagcaaa
    18301 gtgtactgct catcgggtag cagtttattt tcttccagca aggagagcat taacgcgttg
    18361 tcgcgcagtg gactaccgta atcacccagc catatccgct catcactatt tctcggcgtt
    18421 ttcagcgcca gcgcaatcgc ttcttcacca cgcgtcgcat cacccatggt tttcagcgca
    18481 atgccaagtt gcagcagcgg taaaccagaa gcggcatctg cacgatgctc ccagatttca
    18541 cgcagcgcac ccagtggtgc tttttgctga cgggccagca ccagcgcagc gtaagactga
    18601 acggcgaatt tactggcttt gagattatcc gcgtacggga tcgacatcat gcccggatct
    18661 tgtaaatagc gcagcagacg ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa
    18721 ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag ccgtcagcca gtactcttcg
    18781 tcaccgtttt tatcccacag cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg
    18841 gaaatgccga tatcgaccga tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac
    18901 gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca ggccgctggc ggtttgctca
    18961 agacagccgt acggatacgc ttttaactct ttgatataac gtgcaatgtt cagcggtggt
    19021 ttgccgctca acaacaattg cccttccagc gtaacaggcg agaagttttg caatccatcc
    19081 gccggaatcg cccatgtctc accaggctgt aacgccgtac cgtaattaac cgtttgtgcc
    19141 gggaacgccg gacggacgcc gattttccac tgcttatgct gatcggcaac ggtttcaccc
    19201 ggtaacgcta acccgctaat ggtggcctga atttctccat cgccataacc cggcaatgct
    19261 cgcaccggga taaacagcgt agtacgcaca cctggcgcta attcaacggc tgcgggtgaa
    19321 tcgctgacca gttcaagcaa accactggcg gtcagggcaa cgttcagttt ttgcggttta
    19381 tcggtaagat tagtgatatc cagcgtcaga cgcgaggtat cgccactcgc cataaagcgc
    19441 ggcatgttca gttcagcaat caccggtgcg gcaactatca ctttgctttc gttgctaccg
    19501 aaatcatctg ccgtccaggc ttgcgccatg acgcgcaatt caccgttaaa atcgccaatc
    19561 ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta ccgacagcgc ctgctgcgcg
    19621 acaatattga cgtgattgac cggcggttta ccaccacgtt tcagctcatc accatcgcca
    19681 ccgaaacgca gagctgccag acgcccctga ccttcaataa cctgaccgta aatatcgtaa
    19741 atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc acggatctgg cgtaacgtag
    19801 tcagtaatat tcagcacacc actatcaacg gcagacacca gcacattcac ctgtttaggc
    19861 ttctcgccat ttttagtgct ggctttaatt ttcacggtta atggttgatt gggacgcatt
    19921 tttgctggtg tttccagcgc cagatcgagg cgacggtttt catcaccaag cggcagatgc
    19981 aacacaccaa ccgcgcgttt tggcgtcgcg gagcgagatt tatcgccagg acgcaccacc
    20041 agcgtactca aataaagatc atgacgattc caggttttat cgaccggaat cgtcagatcc
    20101 agcccttgag ccggaacatc aatctcttgc caccacagcg gcccttcact ggactcgacc
    20161 atcgcataac ctttacccgc cgttggcgcg gcaatatgca acttaatggt atcgccgggg
    20221 cgataactgg ctttatctag tttcagcgtg acacggtcgg gtcgcactgc gccgctcccg
    20281 tcgctgttgt cctgccagct atagccagcc caaaaacgca cgctactgac cgcttcattc
    20341 ggcgctttga cttccagacg ataagcgccc cactctaccg ggaaactgac cttaccggtt
    20401 tcatccgcct gaagatccag agtttgttca ttttcgatca gatctttttg atcaaactgt
    20461 gactgccagc cttcatcttc tgaccagttc cagtagtaat cgcggcgttc gcgaatcagg
    20521 cgcacctgca agcccgacac ggcttttttc acgccttgcg catcgctata aacgatgtca
    20581 aaagcggcgt tactgccttc atcaacaatg ggctgtttca ccgtgctgtc agtgcgataa
    20641 tcgtaaaccg atttcaaggc gaactgcgga cggagccccg gcaatgcatc ggcaggccag
    20701 atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg attccagcaa actgccctgg
    20761 aaaataacct gcaatgggga atgcgtttcc ttccactggc tttctgtaga aacttcgccg
    20821 cgccctttat catccagcgt caactgaact tcatccagcg tgcgggaaag gttttcggca
    20881 gctatatcgc cgaattcaaa accaggtaag gctgacacag cctcacgcag tgggcgcagg
    20941 aaaagttgcc cttgcaaagt attaccatta gcaggcgcac cgtacaggta gtatcccacc
    21001 acggagaatt tcacttcatc attcggcgta agcggggttt tctcaccggt cagattcagc
    21061 gccatacgct ctggcataaa atcttcgacg tggaaatccc acatccggta ctggttatcg
    21121 cccgtgttgg cgcgaatatg ccacataccg gttgccgcat tgctatcgag tggccaggta
    21181 aagtggtaga ggccattctc cggctgacta acgacgttcc tgagtacctg cccgtcaggt
    21241 ttaatcacgt ctaacttgat gggttgatcg ggcaatgctt taccgtctgc atcgcgcagt
    21301 aaaccattga ggattaccgt ttcacctggg cgataaagat cgcgtgggcc aaacatgaaa
    21361 aactgtttgc tatagcctgg cgcgccagca atgttaaatt ctgctaagtc cagcgccgga
    21421 agttttaaat cgagtagcgt tgtctgaccg tctttacgtg ccagcagtaa tgccgcattt
    21481 ttatcatttt ccagctgcac atgcccctga gcgtcactgg ttgcctgagt cagagtctgt
    21541 cctttctcat ttaataaaga gacttcaatt ccttgctggg ccgcgccgtt ttccagactt
    21601 tgggtaaaga tatccagacg attgtgataa cggtgagctg aaacgccgat atcacttaac
    21661 gtaaacagcg tcgcgggatt actgtaatcg taacgtccag cctgattcat cacagccaga
    21721 tacacgcccg cctgctgaag cggtttgata tcgcccagcg gcagcaataa tttttcacgg
    21781 gtgttacgcg caggattgag atcaaaccgt ccggtgtaga ccagatccgc catctgcagc
    21841 agtttgtctg actgccagtt cgccagcgaa ttgcggtatt cccattggct aatgaatgct
    21901 ggcagagatt ctggcttaac gcggaagaag ttaacatcga cattattaac gttgagcgcc
    21961 attaccggca gcccttcaac gactttgcca ggcagcagcg aaccacggct ggcaaaaccg
    22021 acgctgggtt ggatgtcgcg ggtagttata gttttttcgt aatctttact gaaggttgcg
    22081 ttgttgagtg ctttgacctc cttgccaata gtaacgatca aatcacgttt cggttcgagg
    22141 tggcgtaaac gtagctcttt aagattatct gacagctccc aggcaccatc cactttgccg
    22201 ctttttttat cgacgacatg aataacgcgt gagaaatcct gatccgggtc gagagggatg
    22261 gagaacgtca gcaccagcgt ggctgcacca tcaagttgga cttcagagag atccagcaat
    22321 gtcagcacct taccggcact ctgttgggcc agtttttgtc tctccggcac ggagagtttt
    22381 gctgaactcg cgttttctga agaggccgct ttagtaactt cagaaggcgc atcttttttc
    22441 accgctgttg gcgcgttatc gttgttgtcg caccctgcca gcgccagcat tagcatgcag
    22501 gcggctacgc gtaacttttt catttttcat ccctgccaca atggcccgtt agcaacgtcg
    22561 aataattatt atgcgtgaga atttacgtta tgtaattcag tatcacgccc atacaatccg
    22621 aattttagtg agaattgtgg ctcagacagc caaatcagcc tctttcgctt gtcgcttttc
    22681 actaaacatc cgacaattta gcctaacccc ggcaaaaatg gagatgccta tgtccacgac
    22741 atggtttgta ggagccgact ggctcgccga acatattgat gacccggaaa ttcagattat
    22801 cgatgcccgc atggcgtcgc ctggacagga agatcgtaac gttgctcagg agtatctgaa
    22861 tggacatatt cccggcgcag tgttttttga tatcgaagcg ctttctgatc acacttcccc
    22921 gcttccgcac atgctgccgc gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt
    22981 taaccaggat aagcacctga ttgtctatga cgaaggtaat cttttctcag ccccacgagc
    23041 atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg attctggggg gtggacttgc
    23101 aggctggcag cgcgatgatc tgctgttaga agaaggtgca gtagagctgc cggaaggaga
    23161 gtttaacgcc gcgtttaatc ctgaagccgt ggtgaaagta accgatgtat tattggcaag
    23221 ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct gcacgtttta acgcagaagt
    23281 tgatgaacct cgcccaggtt tacgtcgcgg acatattccc ggagcactga atgttccgtg
    23341 gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat gaactggatg cgatattttt
    23401 tggtcgcggc gtcagctacg acaaaccaat tatcgtcagc tgcggctctg gtgtaacggc
    23461 agccgtggtt ttgttagcac tcgcgacgct ggatgtgcca aacgtgaaac tgtacgacgg
    23521 cgcatggagt gaatggggcg cgcgggcaga tttaccggtt gagccagtga aataagtatt
    23581 ttacaggcaa taaaaaaccg ccgaatttgg cggtttttta ttgctggtct ggttcgcggc
    23641 ctttccagca ggttgtatta ccgtagtaat gcaagcgcgt ctcagcggag acaatactcg
    23701 ccagtaactc tctttttgtc aagcaaaaga gagttattat tgttctgtta gtgtattatc
    23761 cactgcggcc ctttccgccg tctcgcaagc gggcgctggc ttaaggaaag gatgttccgc
    23821 gaccgttaga gcaagcgcgt ctcagcgctt gctatcgcgg caatatcgcc agtggtgctg
    23881 tcgtgatgcg gtcttcgcat ggaccgcaca atgaagatac ggtgcttctg tatcgtactt
    23941 attgtttctg gtgcgctgtt ttcccaagta aataataacc ggagtctctc cggcgacaat
    24001 ttactggtgg ttaacaacct tcagagcagc aagtaagccc gaatgccgcc ctttagggcg
    24061 gcatatttta gattatccgg ttttgtttaa agtcacgcaa aaaaccaccc cagcgacgtt
    24121 catagaatgg cacaatatgt tcggtaataa agtggctaat tcctttttcc ccttttttca
    24181 cctgacaaat atcgattggt tcatcgccag gtaatgtatc ggtcgctaca cttcccgtcg
    24241 cctgaataat ttcttcgata tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg
    24301 cctcttcgtt ctctttaatt gaacaaataa aagcacgctt caccggctta atggttttaa
    24361 ataaggtggt gagtgaatca atcatttgtg ctggcggctc tgcgacttcc gataatatca
    24421 gcgattcacc gccttccagg atttcctggc tgctcagcgg atttccctct tcaccaatca
    24481 acaaactgat ttcacgcggc ataaattctt taccggtcgg cagtttggca ttaaggaaga
    24541 gcgtttcgcc aagggtcatc tcaaacaatg tgcgaacggg cattacgaca aatgcctgtt
    24601 cgtcttcaac cgcctgttga agcgcttcta acgaggtgaa aaaaggaatg acgctggtgc
    24661 cgtcttcttt ttcccagtgc tgtaaatcaa gcgcactatc ttcaaccaca gcctcgccct
    24721 gcgccgccgt accaggcacc cagacggtgg attccagtag agtacggaaa aaggccgggc
    24781 ggtgcgccgg ttcggttgct gctttttcca gcaggtcttc aagttcgttt tttgtttcgg
    24841 acataagaac cacaattcat tcaacgttcg gggcgcaaat gcctgatgcg ctacgcttat
    24901 caggcctaca agggattcgc aatttgttga atttgcagaa tttgtaggcc ggataaggcg
    24961 tttacgccgc atccggcata ttagtttacg ccgttaacag attagctatc gtgcgcacac
    25021 caagtcccgt agcgcccgca gaccactgtt caaccggcgc tttacggtaa gtcgccgagc
    25081 agtcgatatg cagccagcct tgctgatagt tctcaacaaa gtgcgacagg aagcccgccg
    25141 ccgtgctcgc gcctgccgga tacgccgcgc ttccggtatt gttcagttcg gcaaagttag
    25201 acggcagctg gctgcggtgg aattccgcca gcggcagacg ccagaatggt tcgttctctt
    25261 gtgaggcact cgccagcaaa cgaccggcaa gcgcatcgtc aaaactgaac agcgcgtgat
    25321 aatcattacc cagcgcagtt ttcgccgccc cggtgagggt cgccgcatca atgatcattt
    25381 ccggtttctg cgcactggca tcaatcagac catcggcaag caccagacgc ccttcggcat
    25441 cagtgttcat cacttcaact tttttaccgt tgcgataggt gatgatatcg cccagcttga
    25501 acgcattgcc gctaatcagg ttatccgcac agcagaggaa cagcttcacg cgcttgttca
    25561 gtccgcgcgt aatggcaaat gccagcgccc cggtaaccgt tgccgcgccg cccatgtccg
    25621 acttcatcga gtccataaac gcagtctgtt tgatgctgta gccgccggag tcaaaagtga
    25681 tacctttacc taccaggcac gcgtacactg gcgcttcttt atcgccagtt gggttgtaat
    25741 ccagcgccag caataccgga gaacgttctg aaccgcgtcc gacggtgtgc agccccatat
    25801 aaccttgctc acgcagatct tcgcctttgg tgatccgata agtcacacga tcgcccgcga
    25861 cgttgctgat cagatcaaca gcacgctgtg ccagttgcga tggtcccaat tcttctgccg
    25921 gtgcgttgat ggtgtcacgc acccagtcga tgatcatcag gcggttatcc agttcctggc
    25981 gctgggcatc gtccagatcc ggccactcca ctttacgcgt gcctttcggg gctttgtaac
    26041 cttgccagaa tgcccagcag cgatccgcat cccagccttc accgcttaac tgaacatgct
    26101 tgatgcccag accgtcaatc ttgcgcgccg cacgctggat cagccccaga tcgtctgccc
    26161 cgttcaaatg cagggtaatg ccgtcattat taatgctgta agttgctttt tctccccagc
    26221 gcgcgtcggc aggttgggta gagagggtaa tcttcatcgc ttctgtcatt ttagttatcc
    26281 ttctttgtaa aagggccgcc agttggcagc cccaggattt ttccgctaaa tgattcgcgt
    26341 tgcagaaagg cggcaagtga gtgaagcccc aggagcatag ataactatgt gactggggtg
    26401 aacgagcgca gccaacgcat ctgcggcgtg aagcatgacg cggaaattat tcggcctcgt
    26461 ccagccagac taacaaaatc gcttcgagga ttttctcgtt ggatgcctgc gggtcgtcgt
    26521 cgaaatcttc cagatcgcaa atccactgat gcatatcggt gaatcgaacc gttttcggat
    26581 caagatcagg atacgcatcg tacagtgctt cgccaatttc gcggctatcg gtccacttaa
    26641 gtcccatact aacctctgtt aatgctcacg cgcatggttg atagtgtaac gcgggatttc
    26701 gactactaaa tcttcgtcgg taacgcgtgc ctggcagctt aaacggcttt cgggctccag
    26761 tccccaggct ttgtccagca tgtcgtcttc ctgctctgag ctttctggca gcgagtcaaa
    26821 accttcacga acgatgcagt ggcaggtggt gcaagcacag gatttttcac aggcgtgttc
    26881 aatctcgata ccgttacgca gagctgcgtc gagaatggtt tcaccgctat tagcttccag
    26941 aacagcgcca tcaggacaga gatcctgatg aggcaaaata acaatctttg gcatattaaa
    27001 cctcgtccac ggaatggcct ttcagcgcac gacgaaccga ctggtccatg cggcgagcgg
    27061 cgaaatcctg ggtttgtttg tctacgtttt taatcgcttg ttcgatggcg tcaacgtcat
    27121 cgccctgcgc cacttcactc aggtgagcgg cagcatcgtc aatgacctga cgttctgcgg
    27181 cgcttaacag cgcggcatca gcagccagcg cgccgtgcag actttccagc acacgcgccg
    27241 cttctacttt ttgttctgcc agcattcggg cttttacgtc ctgctcggca tagctcattg
    27301 agtctttgat catcgaagcg atttcgctat cggtcagacc gtaagacggt ttgacctgaa
    27361 tagacgcctc aacgccggtg gatttctcca tcgccgtcac gctcaaaaga ccgtcggcat
    27421 cgacctggaa cgtcacgcga atatgcgcac caccagccgg tagcgccgga ataccacgca
    27481 gcgcaaaacg cgccagtgag cggcagtcct gcaccagttc gcgctcaccc tgcattacat
    27541 ggatagacat cgccgtctga ccatctttaa aggtggtgaa atcctgagcg cgggccaccg
    27601 gaatagtggt attacgcgga atcactttct ccaccaggcc gcccatcgtt tcgaggccca
    27661 gcgacagtgg gatcacatca agcagcagca tttcgctgtc tggcttgtta cccaccagaa
    27721 tatccgcctg aatcgccgcg ccaatagcga cgactttatc cgggtcgatg gaagtcagcg
    27781 gtggacgacc gaaaaattcg cctacccgtt cacgcaccag cggcacgcga gtagaaccgc
    27841 ccaccatcac cacttccagc acttcgtcag cttctacacc cgcgtcttta agcgcgcgac
    27901 gacaagccag taaggttcgt tttaccagtg gcgcgatcag ttcattgaat tgttcacggc
    27961 tgatttcgcc ctgccagccc gcaacgttaa cggtcacgga gtccgcatcg ctcagcgcga
    28021 ttttggctgc aatggcggca tccagcagtt cacgctgaac gcggttatcg ctacgatcag
    28081 gaatgcccgc ctgctcgcga atgtaatccg ccagcagatg gtcgaaatca tcgccgccga
    28141 gcgcggaatc accgccggtt gccagcactt caaacacgcc gcgacttaag cgcagaatgg
    28201 aaatatcaaa cgtcccgcca ccgaggtcat aaacagcgat cacgccttcc tgaccggaat
    28261 ccagcccgta ggcgatagcc gcagcggtcg gttcgttaag taagcgcaga acatgcaacc
    28321 ccgccagacg cgccgcgtct ttggtgccct gacgctgggc atcgtcaaag tacgccggaa
    28381 cggtgataac tacaccatcc agctcgcctg ccagggcttc agttgcccgc gccgccagtg
    28441 ctttgaggat gtccgcagaa acgcgcaccg ggttcagcag ccccgccgcc gtttcaatca
    28501 tcggcaggcc gttttcgctg gcctggaatt gataaggcag atgcggatag cgttgctgga
    28561 tatcagccag cgagcgcccc atcaggcgtt taacagaact aatggtgttg gcggtgtcaa
    28621 gcgctgcgtt agtacgcgcg tcataaccca ccgaatgccc ttgctgttga tagtgaacaa
    28681 cagatggcag caggtgacgg ccttcatggt cggctaacgt ttcggcctga ccgctgcgca
    28741 ctgtcgccac cagcgagttg gttgtgccca ggtcaatacc agccgccaga cgacgctgat
    28801 gcggcgcagc actcaaacca ggttcactaa tttgtaataa ggccatgttt agcttccaga
    28861 aattaaaaat cgagcagttt ttcttcgagt tgttcggcac tgctccgcag tttatcgaga
    28921 aaacgcagct tacgcacggt atccgccgcc gcgtcccacg cctcgttgtc taactgttca
    28981 accatcaact gatggcgggt atcaaacatc tttttcacac gtttgataaa gctttccagc
    29041 cgcgcttcat ctttcgcctg ttcgatctcg tccagctctt cgcgcagctc caactgttcc
    29101 atcaggaacg cggtgtcgcg cacagtatgc tgctcgctgg cgagatcaaa accgtgtaaa
    29161 gaaagcaaat attccgcgcg cattaacgga tgacgcagcg tttgccaggc ctggttaatg
    29221 gttgcagatt gctgtacggc ggcgagttgt tccgcctggc ttccgctggc gaatttatca
    29281 ggatgatact gacgttgtag atcctgaaaa cgcaggctca gcgcctgggt atcgagttga
    29341 taacgggcag gcaagccaaa gaaggtgaag taatccataa cattctcagg gctgcgatcc
    29401 gccgcgataa cggcgaatca ggtaaaacaa accccacgcg caggcgacca cggtggggtt
    29461 atcggtatgc gcatcaaacg tggaagcttt cgccgcaacc acactcatct ttgacgttcg
    29521 ggttggtgaa tttaaaccct tcgttcaggc cttcttttac gaagtccagc tgcgtaccgt
    29581 ccagaaattg caggcttttg ccatcgacca cgactttcac gcctttgtct tcaaacacga
    29641 tgtcttccgg cgtcggttcg tcaacaaatt ccagtacata agccatacct gaacatccgg
    29701 aggttctcac gcccagacgc aggccaaacc ctttgccgcg gttagccaga aaggtattta
    29761 ctcgcgctgc tgcactgtcg ctcagtgtaa tcgacataac caaacctcaa ctcttatttt
    29821 gcttcacgtt tgcttttata gtccgcaatg gcggctttga tcgcgtcttc tgccagaata
    29881 gaacagtgaa ttttcaccgg cggcagttca agttcttcag caatatcggt atttttgatc
    29941 gcctgcgctt cgtcgagaga cttccctttc acccattcgg tgaccaggga gctggaagcg
    30001 atagcagaac cgcagccgta agttttaaaa cgcgcgtctt caatgatacc ttcatcgttg
    30061 actttaatct gcaacttcat cacgtcgcca caggccggtg cccccaccat gccgctgccg
    30121 acgttctcgt cgttgttgtc aaaggaaccc acgttacgcg gattctcgta atggtcgata
    30181 actttttcgc tgtaagccat tataaattct cctgattccg ataccgatta atgatgagcc
    30241 cattcgatgc tgttcagatc cacgccctgc ttgtacattt cccacagcgg agaaaggtca
    30301 cgcagacgac cgatggattt acgaactaac tcgatggtgt agtcgatctc ttcttcagta
    30361 gtaaaacgac ctaaagagaa acggatagag ctatgtgcca gctcgtcgtt cagccccagc
    30421 gcgcgcagca cgtaggacgg ttcgaggctt gctgacgtac aggcggaacc tgaagaaact
    30481 gcgaggtctt tcagcgccat aatcagcgac tcaccttcaa cgtagttgaa gctgacgttg
    30541 agaatgttcg gcgcaccgtg ttccaggtca ccgttcaggt aaacttcttc gatatctttg
    30601 atgccgttcc acagacggtt acgcaggccg cgcagacgtt ccatctcggt cgccatctct
    30661 tcttttgcga tgcgataggc ctcgcccatt ccgacgatct ggtgaacagg cagagtgccg
    30721 gaacgcatac cgcgctcgtg accgccgccg tgcatttgcg cttcgatgcg tacgcgcggt
    30781 ttacgacgta catacagcgc accgatacct ttcgggccat agattttgtg accggagaaa
    30841 gacatcaggt caactttcaa ctggctcagg tcgataggca gtttgcccac gctctgggtt
    30901 gcatcaacgt gatagataat gccacgagca cggcacattt cgccgatagc cgcgatatcc
    30961 tgcaccacgc cgatttcgtt atttacgtgc atgatggaaa caaggatggt gtcatcacgc
    31021 atcgctgctt caagttcttt cagatcgata atgccgttac gctgcggtgc caggtaggtg
    31081 acttcaaaac cttcgcgctc cagctgacgg caggtatcca gtaccgcttt gtgttcggtt
    31141 ttgctggtga tgatgtgctt gccttttttc tgataaaagt tggctgcacc tttgatcgcc
    31201 aggttgtcag attcggttgc accagaggta aagacgattt cacgcggatc agcgccgacc
    31261 agatcggcaa tctgattacg ggcgatatct accgcttctt cagcctgcca gccgaaacgg
    31321 tgagaacggg aggccgggtt accaaaggtt ccgtccatcg tcataaactg catcattttc
    31381 tcggcaacac gcgggtccac cggcgtggtt gcggagtagt cgagataaat cggtaatttc
    31441 attgctctat aaactccgta catcactcaa tgcaaggaac caggctaccg gctggatgta
    31501 cgaccgtgtt tacggagcgc gatacgcccc ggcctgattc taatatcttg ttatctttaa
    31561 cctaactgtt tcgggtttca gcaagttgac aaacttacga atctccggga gcttacataa
    31621 gtaagtgacc ggagtgagta tgtgcagtca acgcagatgc aacacgaaac aggacggtta
    31681 aattaagcgc gtaacttaac gtcgatcgcg tcttgtatgc gggtgcgtgg cgcgtcgtga
    31741 gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc gcctaaagta
    31801 atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc gtgggtcagg
    31861 catttatcgc cgccctggca accgccttta ccctgacaac gggtagcatc tacagattcg
    31921 tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc taacagataa
    31981 ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg ggaaaacagt
    32041 tgttccagat aagaaaggga aattccctga cgttcggaaa tatcagccaa cggtaccggg
    32101 cccgcttcag agttgagcgc aacgtcaagc attgcggtca cggcatagcg ccctttagat
    32161 gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt ttattgtaaa
    32221 agtgggggta ttgcatagca gggtcaagtc tgacattccc gagtaaattg gtcaactatt
    32281 tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc gtggcgtttg
    32341 tacctgttgt ccttccagac catgggcggg ttgagggcaa ttattcgcat tagcgctggc
    32401 ctcttcccct caccctaacc ctctccccga aggggcgagg ggaccgtccg tgcatttatc
    32461 tggcttagcc ctcaggcctt attaccttta ttctgctgct ctatagaagc cagaatcccg
    32521 cgcaggatat tcaattcctg gctttccggg cgcgcacggg taaacagacg gcgcaattta
    32581 ttcatcacct gccccggatg gttttcacgg ataaaaccgg ttgccagcag cgtttgttcc
    32641 agatgaccat aaaaacgctc cagatcatcg accagcggat acggcgtctc ttcatgctcg
    32701 acctgctcgc cattttcctg agttgccagc caggccatac gcacttcata ggcaataacc
    32761 tgaaccgcca ttgccaagtt cagcgaactg tattccgggt ttgccgcaat cgcgacatga
    32821 taatggcatt tctgcaactc ttcattggtc aagccgacgc gctcacgacc aaacaccagc
    32881 gccaccgggg tatttgccgc ttcagcgacg cttttcagac cgcattcgcg cgggtcgagc
    32941 atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca ccaccaggct acaaccggct
    33001 aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa tcacatcgct ggcccctgct
    33061 gccagggcaa tcgcctggga gtcgggtttc accagtggat taaccagcca cagattggtt
    33121 aatcccattg ttttcatggc acgggcaaca gaacccatat tgccggtgtg tgacgtctcc
    33181 accagcacaa ttcgaatatt ttgcagcatt gtctttcttc atctaaagat tattcacgca
    33241 tcttatcata aaacgaagac agatgccgat ctcgctgcta tactctgcgc cgttttcccg
    33301 ttctttaaca tccagtgaga gagaccgatg catccgatgc tgaacatcgc cgtgcgcgca
    33361 gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa ccccggacgc tgtagaagcg
    33421 agccagaaag gcagtaacga tttcgtgacc aacgtagata aagctgccga agcggtgatt
    33481 atcgacacga ttcgtaaatc ttactcacag cacaccatca tcaccgaaga aagcggtgaa
    33541 cttgaaggta ctgatcagga tgttcaatgg gttatcgatc cactggatgg cactaccaac
    33601 tttatcaaac gtctgccgca cttcgcggta tctatcgccg ttcgtatcaa aggccgcacc
    33661 gaagttgctg tggtatacga tcctatgcgt aacgaactgt tcaccgccac tcgcggtcag
    33721 ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg ctcgcgatct cgacggtacc
    33781 attctggcga ccggcttccc gttcaaagca aaacagtacg ccactaccta catcaacatc
    33841 gtcggcaaac tgttcaacga atgtgcagac ttccgtcgta ccggttctgc ggcgctggat
    33901 ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg aaatcggtct gcgtccgtgg
    33961 gatttcgcgg caggcgagct gctggttcgt gaagcaggcg gcatcgtcag cgacttcacc
    34021 ggtggtcata actacatgct gaccggtaac atcgttgctg gtaacccgcg cgttgtgaaa
    34081 gccatgctgg cgaacatgcg tgacgagtta agcgacgctc tgaagcgtta atgactcagg
    34141 cgggtgatgt cactcacccg ccctcgcctt tcaggcgcta ttctgaaata cttcctcacc
    34201 gctttacttt ctttcgtcac tctcccacca tctttccccg attaatggat gataaagaag
    34261 tcacaaggaa aggttatggc gctgccagtg aacaaacgcg ttctcaaaat tctgtttatt
    34321 ctctttgttg ttgccttctg cgtttattta gtgccgcgcg ttgccatcaa cttcttctat
    34381 tatcctgacg ataaaattta cggtcccgac ccctggtcgg cggaatccgt cgaatttacg
    34441 gctaaggacg gtactcgtct gcaaggctgg tttattcctt cttcgacggg ccctgctgac
    34501 aatgccatcg caaccatcat tcatgctcac ggcaatgccg gaaatatgtc cgcccactgg
    34561 ccgctggtca gttggttacc cgagcgtaat ttcaacgttt ttatgtttga ttatcgcggg
    34621 tttggtaaat caaaaggcac gccgtcccag gccggattgc tggacgatac gcaaagtgcc
    34681 atcaatgtgg tgcgccatcg cagtgatgta aacccacaac ggctggtgct gttcgggcag
    34741 agtattggcg gggcgaatat tcttgctgtt attggtcagg atgatcgtga aggcatacgt
    34801 gcggtgatcc tcgactccac atttgcctct tatgcaacca tcgccaatca gatgatcccc
    34861 ggcagtggct acttacttga tgagagttac agcggcgaaa attatatcgc cagcgtcagc
    34921 ccgatcccgc ttttactcat tcacggtaaa gctgatcacg ttatcccatg gcagcacagc
    34981 gaaaagttgt atagcctggc aaaagagaca aaacggttga tcctaatccc ggatggcgaa
    35041 cacattgatg ctttttccga tcgtcacggc gatgtttatc gcgaacagat ggtggacttt
    35101 atccttagcg cgttgaatcc gcagaactaa cccatgatcg ctagcacgat aatcattcac
    35161 aaaaccacct taagacatgc taatccactg gtcagaacag tttaagatga gaaaaattct
    35221 gtgacgcttg ccaacatttc tgatgattag cattcccttc gccatctccc tgagcaaact
    35281 ttagctattc ttatcaatta tgcttatggg agatctacag atgatgccta cgcttgctcc
    35341 accatctgtc ctttcggctc cccagcgccg ctgccagatc ttgctgacgc tctttcagcc
    35401 ggggttgacc gccaccatgg caaccttcag cgagcttaat ggtgtggatg atgatattgc
    35461 cagtcttgat atcagcgaaa caggacggga gatcctgcgc tatcatcaac tcacactgac
    35521 gactggttat gacggtagct accgggttga aggtacagtg cttaaccaac gtttgtgttt
    35581 atttcactgg ctacgacgtg gtttccgtct gtgtccgtca tttattacca gccaattcac
    35641 ccccgccctg aagagtgaac tgaagcggcg cggaattgcg cgtaactttt acgacgatac
    35701 caatctacaa gcgttagtga atctctgctc ccgacggctg caaaaacgct ttgaaacgcg
    35761 cgatattcat ttcctgtgtc tgtatctgca atattgtttg ctgcaacacc acgctggaat
    35821 tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa tcctgccttg aatttcaggt
    35881 agcgcaggaa attggacgcc actggcagcg tcgggcgctc cagcctgtac cgcctgatga
    35941 gccactgttt atggcattac ttttttccat gttgcgggtt cccgatccat tgcgtgatgc
    36001 gcatcagcga gacagacaat tgcgtcagtc tatcaaacgt ctggtaaacc attttcgtga
    36061 gctgggaaat gttcgttttt atgatgaaca ggggttatgc gatcagcttt atacccacct
    36121 cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt gataataccc tgccggaaga
    36181 gttcgccaga ctgtacccac gcctggtgcg caccacccgc gcggcgctgg ccggatttga
    36241 aagtgaatac ggcgtccatc tttctgatga ggaaagcggt ctggtcgcgg tgattttcgg
    36301 tgcctggcta atgcaggaaa acgatctgca tgaaaaacag attattctac tgaccgggaa
    36361 tgatagcgag cgagaagcgc agattgagca gcagttacgc gaactaacgt tactgccgct
    36421 caacattaag catatgtcgg taaaggcatt tttgcagaca ggcgctccgc gtggcgcggc
    36481 actgattatt gcgccttata ccatgccatt accgctcttt tcaccaccgc tgatctatac
    36541 ggacctgacg ctgacaacac atcaacagga gcagatccgc aaaatgctcg aatcagcatg
    36601 agggaacaac tttcgggcgc aaaaacattg ccggaagcgc caccagcgcc attacccaga
    36661 acacgccgtg gcccagatat tgatacagga aaccggcgaa aacggtcatg atagcgatac
    36721 tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg gatgacttcg ctaccctggc
    36781 gaacagcaat atagcgcatg gcggccaggt ggcagaccgt gaaggtgccg caatgcaaaa
    36841 tttgcaccac tatcaaccac ggcaacgccg tagttgctcc cataatgccc cagcgcacta
    36901 cgccgcaaat cgccgagatc aacagcatat cgcgagcact acaacggcgg aaaagtttat
    36961 tactcagcgc aaagataatg acttccgcca ccacgcccag cgaccacaaa taccccaccg
    37021 ccgaggccga gtagccagct gcctgccagt aaatggcgct aaaaccgtaa taggccgaat
    37081 gtgccccctg caataaacaa acgcaggcca gaaagcgcca gttctggcga accagcgcca
    37141 accacgcaga ccaaccggtg ctctcctgct ggcgacttgc cccttgtggc tgaatcgtcg
    37201 gacggatgag aaagccgagc agcatggatg ccacgcccaa cgtcaacagc gcgaggatca
    37261 cccgataatc aaacatagtg accagtttgc ccgtcagcgc cgagccaatg acaaacgcca
    37321 ccgagcccca cagtcgcact ttgccgtaat caagcgggaa ctgcttttgc cacgtattcg
    37381 ccagtgcatc ggtcaacggt accagcggtg agaaaaagag gttaaagcca atcatcacca
    37441 gcatcagcca cgctacgtgc gcccccgccc agaaggcgac agcaaagaga agtgtcagca
    37501 gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc gctgacgcgg ggcgcgatga
    37561 gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa taacaggccg atggtttctg
    37621 gcgttaaacc aatcccttta agccagacgc tccagaaagg tagaaaaatg ccgtaactaa
    37681 aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga ttgcaaaacc atgagtccct
    37741 cccgtcagga ggcgttagtc tggggttaat cgaatgttgg tgcaagttga aaaataagaa
    37801 tattcgagcc tgcttcccga tgtctgtaac gccagttcac aagcactaaa aaactgcgat
    37861 ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt tacgcttgcc aaacgttcct
    37921 gcacaatggc gatgaaatcg cgtaatgccg gcttcatctc ccctttcttc cacgccatca
    37981 gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa aacaacctgc ccggtattaa
    38041 aattattcat ataaccgggt atcaaagtga cgcccagccc catgcccacc agattcatgg
    38101 tcaccagaat attcgttgcc acctggacga tatttggctg gctgttttct tgcgcaaacc
    38161 acgctttaac gatcggcgca agcgaaccgg aatacgccgg atcggtactg acgaaattca
    38221 cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc taaagggtga tcaaccggta
    38281 acaccaccac taatggttcg tcaaaaagct ccagataatc aatctccggg ctataaacag
    38341 gatggcgcat taagccgacg tcgagttcac cacggcgaat tttttcctcc tgttgcgtgg
    38401 tgattaaact caccagctca atcaaggtgt ctggctgtct gagacgaaac attggtaata
    38461 cttttggcaa taaattcact tccgccgatg gcacaaagcc aatggttaat tgtctgtctt
    38521 cctgaacaat tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg attgccagcg
    38581 cgtcctggag aaaacattct cccgccgccg tcaacgcgac tttgcgctta tccctcacca
    38641 gtaacggaac accgacacag ttttcaagat cgcggatctg gctgcttaac gaaggctgtg
    38701 aggtatgcag tttttccgcc gcacgggtaa agttcagtgc ctgcgccact gcgacgaaat
    38761 agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt ctcagctggt
    38821 agaaattaac tatttcacat attagcaacc aaccagcaac atccttatga cacaaaaata
    38881 gaaggtcaat acatcttatc tttcaggatt aaaaaatatg accacaccct cagatttaaa
    38941 catttaccaa ctgattgata cccaaaatgg tcgggtcact ccgcgtattt ataccgaccc
    39001 agatatttac caactggagc ttgaacgtat tttcggccgt tgctggttat ttctcgccca
    39061 cgaaagccag atcccaaaac ctggtgattt ctttaacacc tacatggggg aagatgcggt
    39121 tgtcgtagtg cgtcagaaag acggcagcat taaggcgttt ctcaaccaat gccgccaccg
    39181 ggccatgcgt gtgagttatg cagattgcgg caacactcgc gcctttacct gtccgtatca
    39241 cggctggtct tatggcatta acggcgagtt gatcgatgta ccgctggaac ctcgcgccta
    39301 cccacaaggg ttgtgtaaat cccactgggg actaaacgaa gttccttgtg tggagagtta
    39361 taaagggctg atttttggca actgggatac cagcgcaccg ggcctgcgtg attacctggg
    39421 tgacattgcc tggtatctgg atggcatgct ggatcgtcgc gaaggcggca ccgaaattgt
    39481 cggcggcgta caaaagtggg tgatcaactg taactggaaa ttcccggcag agcagttcgc
    39541 cagtgaccag tatcatgctc tgttcagcca tgcttctgcc gttcaggtat taggggcgaa
    39601 agatgatggc agcgataagc gcctcggtga tggacaaacc gcccgcccgg tgtgggaaac
    39661 cgccaaagat gcgctgcaat ttggtcagga cggtcacggt agcggtttct tctttactga
    39721 aaaaccggat gctaatgtct gggtcgatgg cgcagtttca agctattacc gcgaaaccta
    39781 tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg gtcataacaa
    39841 tattttcccc acgctttcat ggctcaacgg cactgccacg ctccgcgtct ggcatccgcg
    39901 cggccctgat caagttgaag tgtgggcgtt ctgtattact gacaaagccg cctccgatga
    39961 agttaaagcc gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg gttttctcga
    40021 gcaggatgac tcggagaact ggtgtgaaat ccagaaattg cttaaaggcc accgcgcccg
    40081 caacagcaaa ctgtgtctag aaatggggct tggtcaggaa aagcgccgcg acgacggcat
    40141 tcctggcatt actaactata tcttttcaga aactgccgct cgtggaatgt accaacgctg
    40201 ggccgatctt ctgagtagcg aaagctggca ggaagtgctc gataaaaccg ccgcttacca
    40261 gcaggaggtg atgaaatgag tgcgcaagtt tcactagagt tacatcaccg cattagccag
    40321 tttctctttc acgaagccag cttactggac gactggaaat ttcgtgactg gctggagcag
    40381 ctcgacaaag agattcgtta caccatgcgc accacagtta acgcgcaaac acgcgaccgc
    40441 cgcaaaggcg tccagccacc gacaacctgg atttttaatg acaccaaaga ccagctggag
    40501 cggcgaatcg cccgtctgga aacgggcatg gcctgggcag aagagccgcc gtcacgcacc
    40561 cgtcacttaa tcagcaactg ccagataagc gaaaccgaca tcccaaacgt atttgctgtg
    40621 cgggtaaatt atctgcttta tcgggcacaa aaagagcgcg atgaaacatt ctatgttgga
    40681 acgcgtttcg acaaagttcg ccgtctggaa gatgacaact ggcgcttgct ggaacgggat
    40741 atcgtcctgg atcaagcggt aatcacttcc cataacctga gtgtactgtt ctgatgaatc
    40801 gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg atcgatacct
    40861 cgcccgtcat cgccctgttc aacgttggcg gcgagtttta tgccattaac gatcgttgca
    40921 gccatggtaa tgcgtcgatg tcagaagggt atctggaaga tgacgccacg gtggagtgcc
    40981 cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg cccgccaccg
    41041 atccgctcac cacttatcca gtacacgttg aaggtggtga cattttcatc gacttaccgg
    41101 aggcgcagcc atgagcgatc tgcataacga gtccattttt attaccggcg gcggatcggg
    41161 attagggctg gcgctggtcg agcgatttat cgaagaaggc gcgcaagttg ccacgctgga
    41221 actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata ttctggcggt
    41281 ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga tcctgactcg
    41341 ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca atgcctcact
    41401 ggttaatact cccgcagaga cgctcgaaac cggcttccac gagctgttta acgtcaacgt
    41461 actcggttac ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca gtgaaggcag
    41521 catgattttc acactgtcaa atgccgccag gtatcctggc ggcggtggcc cgctgtacac
    41581 cgccagtaaa catgccgcaa ccggacttat tcgccaactg gcttatgaac tggcaccgaa
    41641 agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg gcccacaggc
    41701 gctcgggcaa agtgaaacct cgataatgca gtctctgacg ccggagaaaa ttgccgccat
    41761 tttaccgctg caatttttcc cgcaaccggc ggattttacg gggccgtatg tgatgttggc
    41821 atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc aacgctgatg cgggtttagc
    41881 gattcgcggc attcgccacg tagcggctgg gctggatctt taaggaagca cgatgaaaga
    41941 aaaaacgatc attattgtcg gtggcgggca agcggcggca atggctgcgg cctcgctacg
    42001 ccagcaaggg ttcaccggtg agctgcatct gttttccgat gagcgacatc ttccttatga
    42061 acgacctcca ctctcgaaat ccatgttgct ggaagattcc ccgcagttac agcaggtgtt
    42121 acccgctaac tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa ccatcaaaac
    42181 gctgggtcgc gacacacgag agttagtgtt aaccaacggc gaaagctggc actgggatca
    42241 gctttttata gcaatcggcg cggcagctcg accgctgccg ttgcttgatg cactgggaga
    42301 acgctgcttt accctacgcc atgccggtga tgccgccaga ctgcgagaag ttctgcagcc
    42361 cgaacggtca gtcgtgattg tcggtgccgg aactattggt ctggaactgg ctgccagcgc
    42421 cacgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa
    42481 tgcaccaccg cccgtgcaac gctatctttt acagcgccac cagcaggctg gtgtgcgcat
    42541 tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac tgacgctgca
    42601 aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca gcgccaacga
    42661 gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg atgaggcttg
    42721 ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc gtcttgataa
    42781 tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc aaattgccgc
    42841 tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct ggagcgatca
    42901 gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc tttgtcgtgg
    42961 caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc
    43021 ggtcacgctg aatcaggggc gtgagattcg cccaattcgc aaatggatcc agagcggcaa
    43081 aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac tgtaaccagg
    43141 ataattagcg aatatctcaa cgcctggggc gtggcgaggt gcaagagtgt gtattacgtt
    43201 taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgt
//
LOCUS       DAZPLM010000012        42609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPLM010000012 DAZPLM010000000
VERSION     DAZPLM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.012"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            175..1395
                     /locus_tag="ACHVC7_000564"
     CDS             175..1395
                     /locus_tag="ACHVC7_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR2114702.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1368..1997
                     /locus_tag="ACHVC7_000565"
     CDS             1368..1997
                     /locus_tag="ACHVC7_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR2114703.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1998..3158)
                     /gene="ybdL"
                     /locus_tag="ACHVC7_000566"
     CDS             complement(1998..3158)
                     /gene="ybdL"
                     /locus_tag="ACHVC7_000566"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR2114704.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3267..4355
                     /gene="hcxA"
                     /locus_tag="ACHVC7_000567"
     CDS             3267..4355
                     /gene="hcxA"
                     /locus_tag="ACHVC7_000567"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR2114705.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4365..4562)
                     /locus_tag="ACHVC7_000568"
     CDS             complement(4365..4562)
                     /locus_tag="ACHVC7_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR2114706.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4744..6849)
                     /gene="cstA"
                     /locus_tag="ACHVC7_000569"
     CDS             complement(4744..6849)
                     /gene="cstA"
                     /locus_tag="ACHVC7_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR2114707.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7030..7443)
                     /gene="entH"
                     /locus_tag="ACHVC7_000570"
     CDS             complement(7030..7443)
                     /gene="entH"
                     /locus_tag="ACHVC7_000570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR2114708.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7446..8192)
                     /gene="entA"
                     /locus_tag="ACHVC7_000571"
     CDS             complement(7446..8192)
                     /gene="entA"
                     /locus_tag="ACHVC7_000571"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR2114709.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8192..9049)
                     /gene="entB"
                     /locus_tag="ACHVC7_000572"
     CDS             complement(8192..9049)
                     /gene="entB"
                     /locus_tag="ACHVC7_000572"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR2114710.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9063..10673)
                     /gene="entE"
                     /locus_tag="ACHVC7_000573"
     CDS             complement(9063..10673)
                     /gene="entE"
                     /locus_tag="ACHVC7_000573"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR2114711.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10683..11858)
                     /gene="entC"
                     /locus_tag="ACHVC7_000574"
     CDS             complement(10683..11858)
                     /gene="entC"
                     /locus_tag="ACHVC7_000574"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR2114712.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12233..13198
                     /gene="fepB"
                     /locus_tag="ACHVC7_000575"
     CDS             12233..13198
                     /gene="fepB"
                     /locus_tag="ACHVC7_000575"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR2114713.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13279..14529)
                     /gene="entS"
                     /locus_tag="ACHVC7_000576"
     CDS             complement(13279..14529)
                     /gene="entS"
                     /locus_tag="ACHVC7_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR2114714.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14640..15644
                     /gene="fepD"
                     /locus_tag="ACHVC7_000577"
     CDS             14640..15644
                     /gene="fepD"
                     /locus_tag="ACHVC7_000577"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR2114715.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15641..16633
                     /gene="fepG"
                     /locus_tag="ACHVC7_000578"
     CDS             15641..16633
                     /gene="fepG"
                     /locus_tag="ACHVC7_000578"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR2114716.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16630..17445
                     /gene="fepC"
                     /locus_tag="ACHVC7_000579"
     CDS             16630..17445
                     /gene="fepC"
                     /locus_tag="ACHVC7_000579"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR2114717.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17442..18575)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHVC7_000580"
     CDS             complement(17442..18575)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHVC7_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR2114718.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18791..22672)
                     /gene="entF"
                     /locus_tag="ACHVC7_000581"
     CDS             complement(18791..22672)
                     /gene="entF"
                     /locus_tag="ACHVC7_000581"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR2114719.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22669..22887)
                     /gene="ybdZ"
                     /locus_tag="ACHVC7_000582"
     CDS             complement(22669..22887)
                     /gene="ybdZ"
                     /locus_tag="ACHVC7_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR2114720.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22890..24092)
                     /gene="fes"
                     /locus_tag="ACHVC7_000583"
     CDS             complement(22890..24092)
                     /gene="fes"
                     /locus_tag="ACHVC7_000583"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR2114721.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24335..26575
                     /gene="fepA"
                     /locus_tag="ACHVC7_000584"
     CDS             24335..26575
                     /gene="fepA"
                     /locus_tag="ACHVC7_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR2114722.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26750..27370
                     /gene="entD"
                     /locus_tag="ACHVC7_000585"
     CDS             26750..27370
                     /gene="entD"
                     /locus_tag="ACHVC7_000585"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR2114723.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27492..27644)
                     /gene="hokE"
                     /locus_tag="ACHVC7_000586"
     CDS             complement(27492..27644)
                     /gene="hokE"
                     /locus_tag="ACHVC7_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR2114724.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27995..28147)
                     /gene="hokE"
                     /locus_tag="ACHVC7_000587"
     CDS             complement(27995..28147)
                     /gene="hokE"
                     /locus_tag="ACHVC7_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR2114725.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28116..28384
                     /locus_tag="ACHVC7_000588"
                     /pseudo
     CDS             28116..28384
                     /locus_tag="ACHVC7_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28587..29705
                     /locus_tag="ACHVC7_000589"
     CDS             28587..29705
                     /locus_tag="ACHVC7_000589"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR2114726.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29771..30019
                     /locus_tag="ACHVC7_000590"
                     /pseudo
     CDS             29771..30019
                     /locus_tag="ACHVC7_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            30084..30452
                     /locus_tag="ACHVC7_000591"
     CDS             30084..30452
                     /locus_tag="ACHVC7_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR2114727.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30546..31199
                     /gene="nfsB"
                     /locus_tag="ACHVC7_000592"
     CDS             30546..31199
                     /gene="nfsB"
                     /locus_tag="ACHVC7_000592"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR2114728.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31307..32554
                     /gene="ybdG"
                     /locus_tag="ACHVC7_000593"
     CDS             31307..32554
                     /gene="ybdG"
                     /locus_tag="ACHVC7_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR2114729.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32622..33998)
                     /gene="pheP"
                     /locus_tag="ACHVC7_000594"
     CDS             complement(32622..33998)
                     /gene="pheP"
                     /locus_tag="ACHVC7_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR2114730.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34100..37243)
                     /gene="cusA"
                     /locus_tag="ACHVC7_000595"
     CDS             complement(34100..37243)
                     /gene="cusA"
                     /locus_tag="ACHVC7_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR2114731.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37255..38478)
                     /gene="cusB"
                     /locus_tag="ACHVC7_000596"
     CDS             complement(37255..38478)
                     /gene="cusB"
                     /locus_tag="ACHVC7_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR2114732.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38494..38826)
                     /gene="cusF"
                     /locus_tag="ACHVC7_000597"
     CDS             complement(38494..38826)
                     /gene="cusF"
                     /locus_tag="ACHVC7_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR2114733.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38850..40232)
                     /gene="cusC"
                     /locus_tag="ACHVC7_000598"
     CDS             complement(38850..40232)
                     /gene="cusC"
                     /locus_tag="ACHVC7_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR2114734.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40389..41072
                     /gene="cusR"
                     /locus_tag="ACHVC7_000599"
     CDS             40389..41072
                     /gene="cusR"
                     /locus_tag="ACHVC7_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR2114735.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41062..42510
                     /gene="cusS"
                     /locus_tag="ACHVC7_000600"
     CDS             41062..42510
                     /gene="cusS"
                     /locus_tag="ACHVC7_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR2114736.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10233 a        11774 c        10860 g         9742 t
ORIGIN      
        1 tattgaccgt ctaaatgaga gtttttgata tataactaca gacagctact ataactttca
       61 tctatttatt cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat
      121 aattaacagt cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct
      181 atttataaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg
      241 acactcaaca ccctaccccg cgtttgtgtc tcattttctg gcggcaaaaa ctctggttta
      301 atgctgcatc tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt
      361 atcgactggg aggcgcaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg
      421 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
      481 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag
      541 cccccgcaag atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc
      601 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggtggcgatg
      661 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
      721 aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg
      781 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat
      841 caaaatctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
      901 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt
      961 atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc
     1021 atttacgccg gacatgataa tcatttctat gggcatcgga aaatcctcaa gccagaacat
     1081 ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaaa aacagctgag
     1141 cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt
     1201 ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc
     1261 aaagttttac tcaataacga ttactggtgt cgggcattat catttagtcc tacgaaagcg
     1321 aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta
     1381 tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc
     1441 gcatcaaagc gattaatgaa atccgcatgg cgatccatca ggttagcccc tttcgtgaag
     1501 agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat gattacaacc
     1561 caaacaacgt ggcaccaccc gaaaaaaaac tgctgcaaaa atccattgaa atagatggtt
     1621 ttacccaacc cattgtggtc acacatacgg ataaaaatgc catggaaatc gtggatggtt
     1681 ttcaccgcca tgaaattggt aaaggctcat catcattaaa attacgctta aaaggttatt
     1741 tgccggtgat ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact attcgtcata
     1801 atcgcgctcg tggacgccat caaatcaccg ccatgtcgga aatcgtccgt gaacttagcc
     1861 agttgggatg ggacgataat aaaattggca aagagctggg aatggacagc gacgaagtgc
     1921 tgcgcctgaa gcaaattaac ggcctgcaag agctgtttgc cgaccgtcaa tattcccgcg
     1981 cctggacggt taaataacta aagctggcgc aggcgttcag ctgctgccag caacgtcgat
     2041 tccttcttgg caaaacagag acgaatcagt ttatgtggga agggatcggc gcaaaacacc
     2101 gacagcggaa tcgccgctac gccgtgctcc tgcgtcagcc actggcaaaa ctcaacatca
     2161 tccagggtag aaaccgcgct gtaatccacc agcaaaaagt atgtaccttc acacggtaaa
     2221 atctccagcc ggctttcatt taaggcattt accagaatat cgcgcttctg gcgataaaag
     2281 tccggtaacg caagataatg ctcaggttct gcacgtagca tatcagcaag cgccagctgt
     2341 gccggggtat tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg
     2401 ggcgctggcg caacacaata acccactttc cagccggtca tatgataggt cttgccaaat
     2461 gaagaaaccg ccactgcccg ctcacgcagc tgcgaatgcg ccagcacact ggcatggccc
     2521 tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg
     2581 gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc agacagttgc actggagtta
     2641 tgcggagtgt tgaggatcac cagtctggtg cgctcgctta ataatgcggc aaattcctgc
     2701 cagtcaacgc gaaaatgcgg tggttgcagt gccatacgct tcactattcc cccagaaagc
     2761 gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac aaatcacttc atcgccattg
     2821 cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt tacggtgata
     2881 tcgctatcgg catctggttg atagccatac aaacgttccg ttttctgagc aatcgcctcg
     2941 cgcaaggcct gcacgccggt catgggcgcg tattggtttg ccccctgggc aacgtggtac
     3001 gccagccgct cctgtaaata gcgcggacca tcaaaatcag gaaagccttg cgacaggtta
     3061 atcgcctggt gttgctgcgc cagcgcgctc atctgggtga aaatagtggt gccaagttgt
     3121 ggaagtttgc tttgtggaat cagagggtta tttgtcattt ttattgtacc tgacgaagtt
     3181 gcgtgttgaa ggcactataa cacgatgcta gtatttggca atcaagacgt ttagatgtct
     3241 aaataaaaca ataaggacaa cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc
     3301 cggctaacta cttttcacat ccaggaagtt tcaatcacct gaacgatttt ttcactgatg
     3361 aacaactttc tcgcgcggtg tggatctacg gcaaacgcgc cattgctgcg gcgcaaacca
     3421 aacttccgcc agcgtttgga cttccagggg caaagcatat tttgtttcgt ggtcattgca
     3481 gcgaaagcga tgtacagcaa ctggcggctg agtccggtga cgatcgcagc gtggtgattg
     3541 gcgtcggcgg cggcgcactg ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc
     3601 catttgttgc cgttccgacg atcgccgcta cctgcgccgc ctggacaccg ctctccgtct
     3661 ggtataacga tgccggacag gcgctgcatt atgagatttt cgacgacgcc aattttatgg
     3721 tgctggtgga accagagatt atcctcaacg cgccgcaaca atatctgctg gcggggatcg
     3781 gtgacacgct ggcgaaatgg tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc
     3841 cgctaaccgt gcgactgggg atcaataatg cgcaggccat tcgcgacgta ttgttaaaca
     3901 gtagcgaaca ggcgctgagc gatcagcaaa atcaacagtt aacgcaatca ttttgcgatg
     3961 tggtggatgc tattattgct ggtggtggga tggtcggtgg tctgggcgat cgttttacgc
     4021 gtgtggcggc ggctcatgcc gtgcataacg gtctgactgt cctgccgcaa accgagaaat
     4081 ttctccacgg caccaaagtc gcttatggca ttctggtaca aagcgccttg ctaggtcagg
     4141 atgatgtgct ggcgcaatta accggggcgt atcagcgttt tcatctgccg accacgctgg
     4201 cggagctgga aatggatatc aataatcatg cggagatcga caaagtgatt acccacaccc
     4261 tgcgtccggt ggaatacatt cattacctgc cagtcacgct gacaccagat acgttgcgtg
     4321 cagcgttcga aaaagtggaa tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg
     4381 ccgccttttc caccgtagcg cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc
     4441 ggcgtttgat cgggatggtt aacccgcata tgttcgacat agttgtcgta atcaggcata
     4501 ccaatcatca gcttcgccgc ctgacctaaa tattttccgg cttttgccag tgaatcaaac
     4561 atcttggcct ccgctaacca tgttgctgaa agagtgccgg atgcggcgtg aacgccttat
     4621 ccggcctaca aatgctatca acgcgctcgg tcagtcccct cgccccatcg gggagagggt
     4681 tagggtgagg aggaaccctc ggcactatgc caacattcgc caacatcccc cctcactctg
     4741 actttagtgt gcgccttttg cctgcgccac gatctcctcg acattttccg gcattggctc
     4801 atacggcgtt tctttcgccg ttggcttcgg atctttcaat gccgccagtg ccgacttaat
     4861 cgagaacagc gccagaacca ccacgaccac cataaagaag atggttaacc cggcatccag
     4921 acggttgttg aacaccagtt gcgccagctg tgactcagta tactgcgacg gaatattgcc
     4981 gctgtcgatc attgcctgga acttattagc aatggccagg aagccgactt tcgcatccgg
     5041 gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc agcagccagg ccgttggtac
     5101 cagcgccacc caggcgtaac gttgacgttt catcttgaac aacaccacgg cacagagcat
     5161 cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca gagtgttaat
     5221 gccgcccagc ggatcgacca cgccctgatg gaggaagtag ccccacgcca gtacgcacag
     5281 cgctgttgcc agcaggttag caggcagtga atcggtccgc ttcaggccag gagacaccac
     5341 gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cccgcatcta ccgccgtcag
     5401 aataaacaga gcttcaaaca gaatggcgaa gtgataccag aacgccacat ccatcatacc
     5461 acccagcgca ccgtgcagga tgtaagccat ccccaccgcc agcgtcggcg caccgcccgc
     5521 acgggaaatg atcgactgtt cacccacttc gctggcaatc tggttgagcg tatccggagt
     5581 aatcgcaaag ccccagctac tcaccacctg cgcggcagaa gcgaccacat ccgccgtccc
     5641 tgccggagcc agcaccgcca tcgggctgtt catggcgaag tacacgcccg gatcgatgat
     5701 acaggcggaa accagtgcca taatcgccac gaaggattcc attaacattc caccgtagcc
     5761 gataaagcac gcctgccctt cgttcgccag catcttcggc gtggtgccgg aagagatcag
     5821 cgcatggaag cctgacaccg cgccacaggc gatggtgata aacaggaacg ggaacagatt
     5881 accggtccat accgggccag tgccatcgac aaatttggtc agcgcaggca tggtcagcgt
     5941 cggacgcata atcaaaatgc ctaccgccag accaacgata gtcccgattt tcaggaaggt
     6001 agagaggtag tcacgcgggg ccagcagtaa ccacaccggc agcaccgccg ccacaaaacc
     6061 gtagcccacc agcatccagg tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt
     6121 cgggctttct gccacccagc cgccagagat aatggcaaaa ataaggaaca ccaggccaat
     6181 cactgacact tcgccaatac gccccggacg cagatagcgc aggtagattc ccataaaaat
     6241 cgccagcgga atggtgaacg caacggtata cgttccccac gggctatgag tcagggcttt
     6301 caccacgatc atcgccagta ctgcaaggat aatgaccatg atcataaagc aggctaccag
     6361 cgcaatcacc ccggcagttg gccccatctc ttctttgacc agctcaccca gcgagcgacc
     6421 gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg cgagaaccac
     6481 cccggcgagc agccagatca tccccggcag gtagcccatt tgcgctgcca gcaccggccc
     6541 caccagcggc cctgctccgg caatggccgc aaaatggtga ccgaacagca ctttcttgtc
     6601 cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc
     6661 aaccgccagc acatttttgg cgatatacag cccataaaaa cggtaagcga tcagatagat
     6721 acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat ttaaagcaat
     6781 gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt atttccctga
     6841 tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt gtttctataa
     6901 catctttgta cgatttaaca acacggtaac catgtaaatc ggagttgaaa ccaatattta
     6961 acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc tcatttaatt
     7021 ctgtcgggct catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg
     7081 ttcatcgaaa acgatgattt cccagctctg attttgccgc ccaagatgca gcggctggca
     7141 gacgccgcgt accttgcctt cagacaccgg gcgatgatgg gtggcgttaa gttccgtacc
     7201 caccacacac tgcccgtcac gggtcatcat aaatccggcc atcgatccca gcgtttcagc
     7261 cagcgccgcc gacgcgccgc catgcagcag gccaaacggc tgatgagtac gggtatcaac
     7321 cggcatttcg gcttccagca catcatctcc cagacgggta tacacaattc ccagatgcgc
     7381 caccattgtg ttatcgctgg tggcgttcag ttcgtcgagc gttaaatggc gtttccagat
     7441 catgcttatg cccccagcgt tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg
     7501 ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt
     7561 ttccccagcg gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg
     7621 gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc aggcgaaacc
     7681 acattacagc gcacgccgct aaccgccagt tccagcccga cgctcaacgc caggcttttc
     7741 agcgccgctt tcgatgcgcc ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg
     7801 gacgccacag tgacaatcgc cccgccccgc tgacggcgaa actggttcat ggtttgctga
     7861 aacaggttaa acgcaccgcc gacgttaacc gcaaaagtct gctgccagtc ctctttactg
     7921 agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc
     7981 tccgtttcag ctaacagtcg ctgacatacc tgcgcgacct gcgcagcatc ggcaacatcc
     8041 atcacttcgg tcgcaaaggg atattgctcc tgagcgaacg cttgatcaaa accggtaact
     8101 ttcgctcccg cttcaacaaa cgccagcgcc gtggcgtagc cgataccttt acctgcgccg
     8161 gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag agtagcttcc
     8221 accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatcg ccatgcactt
     8281 tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt
     8341 tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggataacc tcacgcagcg
     8401 ccgctttcct ggcggggaca ggtgctggca gtaatccttc agtcatcacc acccggccag
     8461 accgcccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa tcggccagcg
     8521 catccgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac
     8581 agccaatgtg ggcatatacc ccggtaataa tcagctggtt acgtccactc tctttgagca
     8641 tttgctccag cggagaacga tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt
     8701 cggcatctgg cgtcaggcga tccaccacct tttgctgttc cggcgaacgg gtcaggcccg
     8761 gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc ggctgggcgg
     8821 tgtaataaac cgggatattg tgctgtttgc agtagtcgcg cagcgcagca atattcgcga
     8881 tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag tcctgcatat
     8941 catggattaa caacgcggca cgttgcggtt caaaggccca gtcaacttta ttctgcggaa
     9001 tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc gtgttctctc
     9061 cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc gactttcccg
     9121 accgccgtca gcggaagtga atccacacac tccacgcgat ccggtaattt aaattcggca
     9181 ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gcagcggctc ttttaccacc
     9241 agataagcac agcttttttc gcccatcagc tcatcttcca tgctcaccag tgcggcatag
     9301 atcaccgccg gatggcgcag cagcaggttt tcgatctctt cggcagcgat cttctcgccg
     9361 ccacggttga tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata
     9421 gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcactggc attgtgctgc
     9481 ggacttttgt aataaccgcg gaaggtgtac ggcccgcgcg tcatcaggcg tccaacttcc
     9541 ccttgcggca gtggatttcc ttcagcatca gcaacccata cttcatcgtc cggacacatt
     9601 gggtaaccct gggtatggat aattttctcc gcgctatcat caagtcgggt gtagttcacc
     9661 agcccttccg ccatgccaaa cacctgctgc aactggcagc caatctcagc ggaaatacgc
     9721 gccgcaaggg tggcagaaag acgtgcgccg ccgacctgta acagtttcag cgaggcaagc
     9781 tgcgcccggc tttcgccttc agccagcgcc tgcaaccaca ggctgactgc cggcggcacc
     9841 agcgcggtaa cgttaacctg atgttttgca atcaacggga agcaaagcgt ggcgctggga
     9901 tcggccgcca gaacaaccgt tcctccggca agaaagacgc ccagcgatcc tggcgaactc
     9961 atagcgtagt tatgagccgc cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga
    10021 caaatctcga cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc
    10081 ttcggtgtac cggtggtgcc gccggaaagc tggaaatagg ccacttcatc agcaggtgat
    10141 ggcgtagcag taaaaccgtc tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg
    10201 ctgtcgttga gcagttgcac cacgcgaatg gaggaatgtt ctgtgacgaa agtattgagg
    10261 aaatcatccc cgctaaacag cgcatgttgg cgatcggcaa tcagtaacgc cggttcaatc
    10321 tggctggcat aggcgttcag ttcactacgc tgatggctga acaacgccag caccggcgca
    10381 acgcccagtt tcagcagtgc gaaaaaggta atgtaaagtt cagcgacgtt acccagttgt
    10441 accagcgcgg tttcaccggg tttaatgccc tgacggcgta aactacacgc gaggttatcc
    10501 gccgcctgat tcagctcccg gtaactcaac tgtcgctcgc cgtcgataac cgcgatgctg
    10561 tcactcgcag cgtggcgagt cagaatgtcg gtcagcggca aatcctgcca gtagcctttt
    10621 tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct catcctcgct
    10681 ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac
    10741 tcacccaacg gtgacgacgc aggcacaatc cccgctccgg caaacagacg cacctgattt
    10801 tcccgcagct tcgcgcagcg gatggtcacc acccattcgc cgttaccttc gctgtcacac
    10861 caacccacaa tgccgccaaa cagttcgcgg tcaaacggct ccagttcagc aataacctgg
    10921 gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag acaggccaga
    10981 gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg gagttgcgag atgccacagc
    11041 gtcggcgtgg taatcaattg tggagaggag ggaacgtgta actcactact gcgttcgcgc
    11101 agtacctctt tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc
    11161 agcagacgat tacccgcttc gcgatcgagc acttcatccg gctgacgacg cgcggaaccg
    11221 gctaacggaa tggagctaaa acgctcgccg tctttacgta gcagcagttc cgggctggcc
    11281 cccagcagga cgccaccatc agccagcggg acatggaagt tgtaactaac cgggttttgc
    11341 gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt gatatcaatc
    11401 aatcgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca
    11461 accatctgtt caaacgtggt ttgttcagga attgcctggc gttccaccac gttcagcgac
    11521 tggctgcggg taaaacagtg ggctgaggtc tgtttttcct gacgggagaa cgactgccag
    11581 gattcgggaa tatacagcga cgaaggctga cgtggatcga agggaatcgc cccgaccatc
    11641 accggatttt tgatgccctg cgctttggca tcggcaaaca gcgcggcgag tttttgctgg
    11701 aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaagcgggc gaaacatcct
    11761 gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc aagtgttgcc
    11821 atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac aaaatgataa
    11881 aggctttaat aatgattatc atttatattt tcggtcgcta acctaaaagc aaacgcgcac
    11941 tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt tgtggttaca
    12001 atgaaaatga gaagcattat tgatgaattc gcataagcgc aatgtgatgt cctgcgccgt
    12061 tttgccccct ctcccttcca gggtgagggc tggggtgagg gttaatgttc gcaccagtgc
    12121 tggctgttcc cctcacccta accctctccc caaaggggcg aggggacgga ttgtgcgctt
    12181 tgtcgaattt gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact
    12241 cgccccgctc taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt
    12301 agttcaggcc gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga
    12361 aagccagccg cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat
    12421 tgatgctccg gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca
    12481 gggcttttta cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat
    12541 cggcgaaccg agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc
    12601 aaccggcggg gattcggtgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt
    12661 aatcatcaat tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac
    12721 cgggcatgag aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc
    12781 gaaagagcaa atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc
    12841 acacagtgcc aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg
    12901 ctttacgctg gcgaagttgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca
    12961 tgacatcatt cagcttggtg gggaaaatct ggctgctggg ttaaacggtg aatcgctatt
    13021 cctgttcgcc ggtgatcaga aagacgccga tgcgatttat gccaatccac tgctcgcgca
    13081 cctgcctgca gtacaaaaca agcaggttta tacgctggga accgagacgt tccgcctgga
    13141 ttactacagc gccatgcaag tgctggacag acttaattca ctgttaaaaa aagattgata
    13201 aaacatacaa ttagagcact aataatcccc tcgccccttt ggggagaggg ttagggtgag
    13261 gggaaaatgc acctttagct aactgtcaga cgctgtcacc tgcggcggcg tctggcgaaa
    13321 acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc
    13381 gctcgcgctt gcggaagcaa ccggtgtcat catcgcgccc aaaccaccca acagcgccgc
    13441 gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa ccgttaatcc gccctaacat
    13501 cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa ccgcgctcag
    13561 ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag
    13621 accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcact
    13681 atgtgccagc ttcccgctgg ttaacgcacc aatagccgca ccgagcggga tcgccgcata
    13741 gagaaaacca atctgtgccg ccgacatctg ccagttgtca gccagcgccg gatacagtac
    13801 ccgcaccgcg ctcgccatcg tcaataaacc acccagcaac gcaatccctc ctaccagcgg
    13861 gctggcgagc agaaaacgaa atcctgccag taatgatttc aacggatgct cgcgcggctg
    13921 cggtggcggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa acgtgcccgc
    13981 cgccgccagc ccgtagttcc aggctacgcc gccggtcgcc agcaataaac cgccaatcat
    14041 gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa
    14101 gttttcacgc cctaccagtg caggtgttgc cgccagtagc gccgtaacac caagtgatgc
    14161 gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct ccggcagcag
    14221 cgcgttcagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    14281 ttttttgcgc tcatagcgat ccgccagcac accgccaacc atcaggccaa caaacatcgc
    14341 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14401 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    14461 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    14521 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    14581 gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta
    14641 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga
    14701 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgcg tcagtagtgc
    14761 tggaggcctt ctcaggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    14821 tgccgcgcac ccttgccggt ttacttgcag gcggtgcgct tgggcttgct ggggcgttaa
    14881 tgcaaaccct cacccgaaat ccacttgccg accccggctt gcttggcgtg aacgccggag
    14941 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcatccgcg caggaacaac
    15001 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt atcggcagtc
    15061 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcgg
    15121 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac gatcaactgc
    15181 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    15241 tcccggttct gatcgccgga gcaactgcgc tattactgag tcgcgcgctg aacagtttga
    15301 gcctcggcag tgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttgattg
    15361 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15421 ttatcggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt
    15481 cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc
    15541 gggtgattgt gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg
    15601 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggtgc atgatttacg tctctcgccg
    15661 attactcatc acctgtttgc tgctgatttc cgcctgtgtg gttgcaggta tctggggatt
    15721 acgcagcggt gccgtcacgc tggaaatctc gcaggtattc gccgcgttga tgggcgatgc
    15781 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    15841 gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt cagtcgctga tgcgtaaccc
    15901 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtggc
    15961 gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat
    16021 tgtcacttct ctgttggtct ggctgctcgc ctggcgtaac ggtatcgaca cctttcggtt
    16081 gattattatc ggcatcggcg ttcgcgccat gctggtggcc tttaatactt ggctgttgct
    16141 gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgccggat cgctcaatgg
    16201 cctgacgtgg gcaaaaactt cgccttccgc acccatcatc atattgatgc tcattgccgc
    16261 cgccttgctg gtgcggcgaa tgcgcttgct ggagatgggc gatgataccg cctgcgcgct
    16321 gggcgtcagc gtcgagcgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc
    16381 tgcggcaacg gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc
    16441 ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt gcggagcgct
    16501 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    16561 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    16621 tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac cctgggatat
    16681 ggcaaatata ccgttgcgga aaatctgact gtagaaattc ctgatggtca cttcacggca
    16741 attatcggac caaatggctg tggtaaatcc acgttactgc gtaccttaag ccgcctgatg
    16801 acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    16861 gaagttgcac gccggattgg tctgttggcg caaaatgcta ccacgccggg cgatatcacc
    16921 gtgcaggagc tggtggcgcg tggacgttat ccgcatcaac cgctgtttac ccgctggcgc
    16981 aaagaggatg aagaagcggt aacgaaagcg atgcaggcca cgagaataac tcatctggca
    17041 gatcaaagcg tggataccct ttctggcgga caacgccagc gagcgtggat cgcgatggtg
    17101 ctagcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct ggatatcagt
    17161 catcagattg atctactgga actgttaagc gaactgaacc gcgagaaagg ctataccctg
    17221 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    17281 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaactgatt
    17341 gagcgcattt atggtctgcg atgcatgatc attgacgatc cggtagccgg aacgccgctt
    17401 gtggtgccgc tcggacgaac ggcaccttca accgcaaata gttaaactaa gtggtctgcc
    17461 atcatggcat cctgttttct ggatgccatc gcatagcgca ataacacgct accacaagcc
    17521 accatcccgc cgatcagcgc ggaaaggatc acaataatcg ccttacccgg accgtctttt
    17581 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgaca
    17641 tttgcttttg ttaattgctc gacaagatac tgccgattac gtaattcacc gttcagttcc
    17701 gcaacgtcag taaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga
    17761 gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtatac gggcttttta
    17821 attcctgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    17881 tgatttttcg ttttaatgcg atcctgagcc agtttttctt tttcaaactg ggttttgatc
    17941 tccagtttat tacggacgtt ttctatcgac tctttcacca ccaacgcaga gatataatcg
    18001 atatacccgc tcaaaacggt ctgcgcctct ttactggttg gcgcggtaaa acttagcgtc
    18061 caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    18121 attttttcgc tcaatgcaac aatagcgcga tgcaaatcca gttcatcgat tttcgcctct
    18181 tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaacagac
    18241 tgaaacttct tgataaacag gttaaatgca ccgccacggt cgatattaac atcgagatca
    18301 agaacgcgga gtttggtaaa ggttttctcc aggtcctgcc actgaatggc ttcggcggga
    18361 gtaataacgg ctgagctggt ccatttttgc ggcaggatga aagagatcag taaacctgcg
    18421 caggcaaatg caaaaacgac cgccatgacc gtttttttgg cccgccataa aacttcgatt
    18481 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc agaaaaatga
    18541 gcgtcacttc cctgtttaat attcaatgat gacatggggt ataacctgaa aatgagtcca
    18601 ataggggaaa atctccacgg cgctcacttt accagttggt gagaattatc cgaagctgaa
    18661 gtttgtaaat tccttccact gaatgcaata ttcactctat ttaagagaaa tattcatcaa
    18721 acctataaat caaacaaata aaaaacccat aattattttc tgtaattatg ggtttataga
    18781 taatattaat ttacctgttt agcgttgcgc gaataatcgg cccaattttt acaaacgccc
    18841 ctggagagat aatatccaca tgcgcacaat cctgacgata gatatccagc tccgcaatcc
    18901 acggcgacca ggcgcgttca ggactcatac cttcctgaag cgtgcgttca gcaacaaaca
    18961 acgtcgcttt accgtcaaac ggtacgctat gagcagtcgt cagcaggcgc acagcatcag
    19021 cgtagttgcc ttcaatggtg gtaaacaact ccgttgaagt acttccctgc tgtgccgcca
    19081 ggaaggcctc gcgctcgcgg ttaatctccg ccagcacttc cgggtccaga ccattagctt
    19141 ctttttcctg ccagttttgc gtttctggcg gccaggtatc cagcaagcca agaaatgcca
    19201 cctgttcgcc acgggcacgc agtcgcgccg caataccctg cgccagcgta ccgccaaggg
    19261 aataccccag caggtaataa gggccgtgcg gttgttgctc aagtaacgtt gccagatgcg
    19321 cttcgcagac ttcatccagg tttgtcgctg tctgcatggg tccatgaggg cgcggcgact
    19381 gaatgccaat aatcgaccat tgtggatcga gataacgcga gagtacgcta aactgccagg
    19441 caaaaccgga cgccggatgg aaacaaaaca gcgtcgggcc attaccttca cgcaacggca
    19501 gaatggtttc gaatcccatg cgccgggagc tgtcctcttc accatcaata atcgttgcca
    19561 gtttggcgac ggttgacgcg accatcacct gccccggcgt cacctgacga gcaaactgtc
    19621 gacttaactg cgctgccagt ttcattgcca gtagcgaatg accgcccagc gcaaagaaat
    19681 cagcatcggc atcctgcacg tcacaaccca gcaacgacgc gaatgccgcg gcgataatcg
    19741 tttcactgcc cgctttcggt gcacgtcccg acgcttgtgt cttcagttca ggcaacggta
    19801 aggctttgcg atccagcttg ccgttggcgc taagaggtaa ctgtggaagt tgcagcagaa
    19861 ccaccggtac catatgtggt ggcaatgttt cacgaagctg cgcctgcaat gcgctggtat
    19921 ccaacggcag gcccgattgc gacaccagat agcccaccaa ctgacgcgca tcaccaccag
    19981 tggctgccgc ctggttaatc acacaggcgt gggtaacggc ttgttcgaca tccggcagcg
    20041 cctgcatcac gcgatcgatt tcgcccagtt cgatacgctg cccgcgaatt tttagctgat
    20101 cgtcactgcg cccgaggtac tccaccgcgc cgttatccag ccagcgggca acgtctccgg
    20161 tacggtacat ccgttcgcca ggagcaaaag gatcggcaat aaagcggctg gcggtcagat
    20221 cggggcgtcc gagatagccc tgcgccagtt gaatgccggt aagatagaga tcacccgcca
    20281 cacccggcgg caccggatgc atcatcgcat caagaatacg cagccccatg ttccacaccg
    20341 gataaccaat cggcacactg ctgccgcgca cctgtgccag ttcctcgcca aaagccggat
    20401 accagctgac atctaccgcc gcttccgtcg ggccatacag attatgtaac ggcgcaccgg
    20461 ttaactgttg ccattcacgg cataaatcag ccggtaaggc ttcaccacta cagaaaacct
    20521 gtttcaacgt cgcgcaactc tggcgagcgg tttgcggagt cagcgaggca acaaatgccg
    20581 ccagcatcga cggcacaaag tgcgtggtcg ttacgccata ttcggcaaag aattgctgca
    20641 tagcgagcgg gtcacggtgc gcttccggtt cagccatcac cagttttgcc cctgcgataa
    20701 acggccagaa aaactcccac accgagacat caaaactgca cggcgttttt tgggcaacga
    20761 catcttcgcc tgtaagcggg tagtgatttt gcatccataa caggcggtta acgatagccg
    20821 tctgcccgac cattacccct ttcggcctgc cggtggagcc agaggtaaag atgatataag
    20881 ccgtgtggtg cggttgtgaa agttgcagcg gcgcactgcc ctggggggta agcggggcgt
    20941 tatagcaaag gcttgttaaa ttgggaacat cgctaaagcg cggcagttga tcgtcggtgg
    21001 taattaacag cgacggacgc gcatcttcca gcatcatttt caggcgatcg tccggatagc
    21061 cggtatccag cggtagccag gccgcgccag cttcaactat tgcatggagt gccagggtca
    21121 aaaagaccga gcgcggtagt gccaccgcca cgctgtcccc tggtttaacg ccgcgctcac
    21181 gcagcagatt cgccagcgcc accacctgct cgcgcatttc ccgatagctg aacaggtaac
    21241 gcgcatctgc cagcgccgga gcatccggtg tttttgccgc ttgttctgcc accagcgcgc
    21301 tcagcgtggt ttctggaatc tcaacctgag tggcgttgat ctgcgccagc tgcgcatact
    21361 cacctggcag cataatatcg acatcgccgc acaacagcgc cggatccgcg gcgaactggg
    21421 caatcagcat tttcaggcgt tcagcatgct ggattaacgt tggctcatcg taacgctgtt
    21481 tattggcgag gatctcaata ctcaaatcac cgtgttcatc cgggaacagg gccagttcaa
    21541 ggtcattaac cggaccggtt gccagggtat gggtttgcgc ctgaacacca ggaatatcca
    21601 gttggtaatc aaatacctta atattgagta ccggaccaaa cagcggttca tcacctgccg
    21661 ctcgcccgct gtcacggaca atttgttcgg catcgtaacg ttgatggcga cgcatttttt
    21721 tcagttgagc cgccagtcgg gttgccagtt ccggcagcgt ttcttgcgcc gcaatgtgaa
    21781 tacccaacgg taaaacgttg agcacgggtc cgctagccgt cagcgccgcc gagcccagtc
    21841 gacgcataaa gataaatccg gcggcatagt ccatgcgatt gcacaatcgc cccagccaca
    21901 aggctgccag cgcaagggct aaatcggtac gctgcacacc tgaaagttgc gtagccagct
    21961 ggcggaattc cccgtcggta aattccagtt tcaggcgcag aatatctgcc gaagcgctgc
    22021 gccccggtaa aggtgccgga gaaagtgacg cgggcggcgg cagttgacga cgctgttctg
    22081 cccagaatgc cgcatcacgc tgccaggctt cgctttcgcg gtattgctgg tactcttcca
    22141 ctacatcagc gaaaggcgta aacggcgaag caggcgttgg ttcgccacgc agccatgtgc
    22201 agtaaatatt ggcgatctgg cgagtaatgg ccgggaaact gaagccatcg accagcaaat
    22261 ggtgataacg ctgataccag taccagcggt tatccgcaac atgtatcagc tgatgaaaga
    22321 ccagtggttt accgctatcg acgcgcaaat cttgttgcaa atccgtcagc attaatgcct
    22381 gcgcagtacc gtgcggatca atattggttc gcaggtcgat aatttctggc tgttcgaata
    22441 tcagcgcatc atcgacccac tgccagactt cgccgttatc ttccgtaaaa cgcatccgca
    22501 acgtatctgc ttgcgctagt ccggcaacca ccgcgcgggc cagtaatggc gcatcaacat
    22561 ctccagttaa ctcaacgtaa tgcgccacgc tccaggcgga gggtaaatcc gacagttttt
    22621 ctgccatcca gatgccgggc tgtgcggcga ccaaaggtaa atgctggctc attgtgcctc
    22681 ctgcaactgg gtgaaattgg ccggtgtcag agtacgccag tggacttcca gccactgctg
    22741 gcaggacgcc tgtgactgcg gctgacacac aatgtcccag cctgccgata agaggcattg
    22801 ttgcggccac agactgaatt gcccctgcgc attgcgcaaa atgtaaaacg ctccctgcgg
    22861 atcatcgaag ggattactga atgccatact caactcctgt catggaaaag tggttgccag
    22921 aggtcgatta acccctgcat caagccaccg cgccaacaaa gcgcatcatg tccgccgtca
    22981 acctgacgcc agaaaatgga ttcttttata gggtgcaatt gcgcatacag cgcctgattg
    23041 gcccgcatga tcatcggctc gcgaataccc gcttccagca caatgcgcag accttcggcg
    23101 ctaacttcac cagcttttag cttttcaagt aacacgccct cttgctgccc gtcccgatgc
    23161 ggccaccagt acgatcctga ctggcttaat acacagccaa agcgttcagg ccagtgcagt
    23221 ccggcataca gcgcggacag cccaccaaaa ctctgcccgg cgaccacggt gcgatcggcg
    23281 cgatcgctaa aaggggcaat agctttcacc aggggtaata actcttgctg tactgccagc
    23341 cagaaatccg cattacacgg cagttcgtgg gcgcggtgcg tggtgtcgat agcgtcgatc
    23401 aacacataca cggcgggagg aagttgctga cgatgggtca gcgaagtcag cacaggccag
    23461 acgggcatac tttgcgccca aaattcgcca tcgagcaaaa ctgccagcgg acgttcttct
    23521 gctgttacat cgccggtggt aaaaatccat acacgacgtg aatttttcaa ccgttcactt
    23581 ttccagataa tttctttggc tgctatttct ggcacttgcg gacaatccca tcccggttgc
    23641 agaggcgctt gcggcatttc gagtgcagaa acagcgtgcc ctcgcccgcc tttccagctt
    23701 tgtggattca gcggatcggc tattgcctgg ggtaatagtt ttcgccagcc ttcacgtaat
    23761 tcgaggcgat cgggggatgg tgcagaaaaa atgtcatcgc gttcggtggg aataaagcag
    23821 tagctgccgc gccagttggc attgagttgt gtcgtccact gccagacatc agtacctgca
    23881 attcgctgca tcgactgggg ctggctgttc tgatgatgat cggtcacacc agtgatgtag
    23941 atccatacgc gctttatcgt cgagtattct tcagaacctt ggggatcacg ccaccagaaa
    24001 gtgacctcaa acatttcgtc attcagacgc tgccattccg ggccatgttt cgactgccac
    24061 cagctctcac ttcctacttt taacgccgtc acaccataac cccatgttta ctgtgcaatt
    24121 tttcattgat tgcagaaata tattgataat attattgata accatttgca tttgcaatag
    24181 cgtaatggcg cgccgtggga agcgcggaca tttattaacc aactgcactg cgtgtctttc
    24241 aggatcaaag gttttcgcgg tagcgggatg cgtcgtgttg aggacgacca tgcccgacag
    24301 ttgcaattcg tggcaaaaat gcaggaataa aacaatgaac aagaagattc attccctggc
    24361 cttgttggtc aatctgggga tttatggggt agcgcaggca caagagccga ccgatactcc
    24421 tgtttcacat gacgatacca ttgtcgttac cgccgccgag cagaacttac aggcgcctgg
    24481 cgtttcgacc atcaccgcag atgaaatccg caaaaacccc gttgcccgcg atgtgtcgga
    24541 gatcatccgt accatgccag gcgttaacct gaccggtaac tccaccagtg gtcagcgtgg
    24601 gaataaccga cagattgata ttcgcggtat gggtccggaa aacacgctga ttttgattga
    24661 cggcaagccg gtaagcagcc gtaactcggt gcgtcagggc tggcgtggcg agcgcgatac
    24721 ccgtggtgat acctcctggg tgccacctga aatgattgaa cgtattgaag ttctgcgtgg
    24781 tccggcagct gcgcgttatg gcaacggcgc ggcgggcggc gtggttaaca tcattaccaa
    24841 aaaaggcagc ggtgagtggc acggctcctg ggacgcttat ttcaatgcac cagaacataa
    24901 agaggaaggt gccactaaac gcaccaactt cagcctgacc ggtccgctgg gcgacgaatt
    24961 cagcttccgc ttgtatggca acctcgacaa aacccaggct gacgcgtggg atatcaacca
    25021 gggccatcag tccgcgcgtg ccggaacgta tgccacgacg ttaccagccg ggcgcgaagg
    25081 ggtgatcaac aaagatatta atggcgtggt gcgctgggat ttcgcgccat tgcaatcgct
    25141 ggaactggaa gcaggttaca gccgccaggg caacctgtat gcgggcgaca cccagaacac
    25201 caactccgat tcctataccc gctcgaaata tggcgatgaa accaaccgtc tgtatcgcca
    25261 gaactactcg ctgacctgga acggcggctg ggataacgga gtgaccacca gcaactgggt
    25321 gcagtatgaa cacacccgta actcgcgtat tccggaaggt ctggcaggcg gtaccgaagg
    25381 gaaatttaac gaaaaagcga cacaggattt cgtcgatatc gatcttgatg acgtgatgct
    25441 gcacagcgaa gttaacctgc cgattgattt cctcgttaac caaacgctga cgctgggtac
    25501 ggagtggaat cagcaacgga tgaaggactt gagttccaat acccaggcgc tgaccggggc
    25561 gaacaccggc ggcgctattg atggtgtgag tgccaccgac cgtagcccgt attcaaaagc
    25621 agaaattttc tcgctgtttg ccgaaaacaa catggagctg actgacagca ccatcgtaac
    25681 gccggggctg cgtttcgatc atcacagtat tgtcggcaat aactggagcc cggcgctgaa
    25741 catatcgcaa ggtttaggcg atgacttcac gctcaaaatg ggcattgccc gtgcttataa
    25801 agcaccgagc ctgtaccaga ctaacccgaa ctacattctc tacagtaaag gtcagggctg
    25861 ctatgccagc gcgggcggct gctatcagca aggtaacgat gacctgaaag cagaaaccag
    25921 catcaacaaa gagattggtc tggagttcaa acgcgacggc tggctggcgg gcgtcacctg
    25981 gttccgtaac gattatcgca ataagattga agcgggctat gtggctgtag ggcaaaacgc
    26041 agtcggcacc gatctctatc agtgggataa cgtgccgaaa gcagtggttg aaggtctgga
    26101 aggatcgtta aacgtaccag ttagcgaaat ggtgatgtgg accaataaca tcacttatat
    26161 gctgaagagt gaaaacaaaa ccacgggcga ccgtttgtcg atcatcccgg agtacacgtt
    26221 gaactcaacg ctgagctggc aggcgcggga agatttgtcg atgcaaacga ccttcacctg
    26281 gtacggtaag cagcagccga aaaaatacaa ctacaaaggt cagccagcgg ttggcccgga
    26341 aaccaaagaa atcagtcctt acagcattgt gggtctgagc gcgacctggg atgtgacgaa
    26401 gaatgtcagt ctgaccggcg gcgtggacaa cctgttcgac aaacgtttgt ggcgtgcggg
    26461 taatgcccag accacgggcg atctggcagg ggccaactat atcgcgggtg ccggggcgta
    26521 tacctataac gagccgggac gtacgtggta catgagcgta aacactcact tctgatgcta
    26581 acgtcagatt gttgacaaag tgcgcgtcgt tcatgccgga tgcggcgtga acgccttatc
    26641 cggcctacaa aaatcttgcc aattcaatat attgcaggac catgtaggcc tgataagcgt
    26701 agcgcatcag gcagttttgc gtttgtcatc agtctcgaat atggtcgata tgaaaactac
    26761 gcatacctcc ctcccctttg ccggacatac gctgcatttt gttgagttcg atccggcgaa
    26821 tttttgtgag caggatttac tctggctgcc gcactacgca caattgcaac acgctggacg
    26881 taaacgtaaa acagagcatt tagccggacg gatcgctgct gtttatgcct tacgggaata
    26941 tggctataaa tgtgtgcccg caatcggcga gctacgccaa cctgtctggc ctgcggaggt
    27001 atacggcagt attagccact gtgggactac ggcattagcc gtggtatctc gtcaaccgat
    27061 tggcattgat atagaagaaa ttttttctgt acaaaccgca agagaattga cagacaatat
    27121 tattacacca gcagaacacg agcgactcgc ggactgtggt ttagcctttt ctctggcgct
    27181 gacactggca ttttccgcca aagagagcgc atttaaggca tcggagagac aaactgaagc
    27241 aggttttctg gattatcaga taattagctg gaataaacag caggtcatca ttcatcgtga
    27301 gaatgagatg tttgctgtgc actggcagat aaaagaaaaa atagtcataa ctctgtgcca
    27361 acacgattaa ttgacaacat ctggtacgat tcgcccgcag ccatcactga ctgcgggcga
    27421 aagtgtaaag caggtgcctt accactctga cctgacaacc ggatatgcag ggattgctcc
    27481 ccgcacaacg gctacttctt cggttcgtaa gcgagaacag ccttaaactc aatattacgt
    27541 tcctttacgg tgaactcaca cagcgagtct ccgaccagaa gcgtaaatcc cagcaccgtc
    27601 aaacacagca ctatgactgc cacaagggca tatttcgtca gcatgtttat attgcctcca
    27661 tgcgaagaag ggactaccat ccgagttgtt gaggttcgga gtggcagccc caggattgat
    27721 agaaatatct cctggggctt catctttcca gacctcagag taaacctgaa accggaaagc
    27781 ttcaggcacc cgccgttatc ttacccttcc tcagcaaaat atctgtttat ttatacagta
    27841 agtcctgttt cggacttctg ttggcaacac tcgttattgc tcgtaaaacg aaatcgcccg
    27901 cagccatcac tgaccgcggg cgaaagtgta aagcaggtgc cctaccatcc tgacctgaca
    27961 accggatatg cggggattac tccccgcaca acggctactt cttcggttcg taagcgagaa
    28021 cagccttaaa ctcaatatta cgttccttca ccgtaaactc acacagcgag tctccgacca
    28081 gaagcgtaaa tcccagcacc gtcaaacaca gcactatgac tgccgcaagg gcatatttcg
    28141 tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt gttcgggttt
    28201 ggagtggcag ccccagggtt gatagaaata tctcctgggg cttcatcttt ccagacctca
    28261 gagtaaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct tcctcaacaa
    28321 aatggctgtt tatttataca gtaaatcctg tttcggactt ctcactcctc atttaatcaa
    28381 ctgaagctga gcccgttttt tctgcatgcc tcaatcgtca aaattcttcg ccatcagcca
    28441 gctctgccat aacttgccac attgaaaaag tagccaaatt gcttcagtca ccatcatcaa
    28501 aaaaagaaac attccagaac ttccgcatct cgcccagccg ctccgacttc tatactgaat
    28561 agaaaacgcc aacataagag aaacctatgc cattacccga ttttcatgtt tctgaacctt
    28621 ttaccctcgg tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc
    28681 aggactcttc aatgctgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc
    28741 acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg
    28801 ctgccgggca attttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc
    28861 tggaaatttg cggcggtggc acacacccgt ttcagaaatg gcagcgtcag gaggtatgcg
    28921 ataacgagcg ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg
    28981 tttttggtca gcatgtccat gttggctgcg ccagtggcga tgacgccatt tatttgctgc
    29041 acggcttgtc acggtttgtg ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc
    29101 aaggaacgga tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcccgata
    29161 atggcccgat gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgtttga
    29221 gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc
    29281 attttggcac ggtggaggtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa
    29341 atatggcggg attaattcag gctaccgccc actggttact gacagaacgc ccgttcaaac
    29401 atcaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgt cgctatgggc
    29461 tggaaggcgt cattaccgat ccgcacactg gcgatcgtcg accgctaacg gaagacacct
    29521 tgcgattgct ggaaaaaatc gccccttccg cacataaaat tggtgcatcg agcgcgattg
    29581 aggccctgca tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg
    29641 tcgccgatgg tggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggccgatg
    29701 actaagtagg gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt
    29761 taacaatgtt atgaaacacc ctttagaaac cttgactacc gcagcaggca ttttgctgat
    29821 ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa
    29881 actggtgacc atgttccatc tgatggatct tagctagctt tacactttat tgttttgtct
    29941 gtggttttta gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg
    30001 gttctcgctg gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt
    30061 ctccttcgcc taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc
    30121 tggcccttga gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt
    30181 ttaaaattgg cggcaaaatt tttatgctgt cgtcggagct gcgcggcgtc ccctttatca
    30241 atctgaagtc cgatccacaa aaatccctgt taaatcagca gatatacccg agcattaagc
    30301 cagggtatca catgaataaa aagcactgga tttcggtgta tcccggcgag gaaatctccg
    30361 aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta
    30421 aacgcgatca aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg
    30481 cttaccattt ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag
    30541 tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca
    30601 gtaaaaaact taccccggaa caggccgagc agatcaaaac tctcctgcaa tacagcccat
    30661 ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc
    30721 gtgttgccaa atccgctgcc ggtaattatg tgttcaacga acgtaaaata cttgatgcct
    30781 cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg
    30841 ttgaccagga agatgctgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata
    30901 aaggtcgcaa gttcttcgct gatatgcacc gtaaagatct gcatgatgat gcagagtgga
    30961 tggcaaaaca ggtttatctc aacgtcggta acttcctgct cggcgtggcg gctctgggtc
    31021 tggacgcggt acccatcgaa ggttttgacg ccgccatcct cgatgcagaa tttggtctga
    31081 aagagaaagg ctacaccagt ctggtggttg ttccggtagg tcatcacagc gttgaagatt
    31141 ttaacgctac gctgccgaaa tctcgtctgc cgcaaaacat caccttaacc gaagtgtaat
    31201 tctctcttgc cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc
    31261 ctgtttcagc cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat
    31321 atcccaggtt gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat
    31381 tttcggtatt atttttctga ccgccgtcgt ggtgcatatt attttgcatt gggtggtact
    31441 gcggaccttc gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa tcataaccca
    31501 gaataaactc ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc
    31561 ggtattctgg ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt
    31621 gtggatcatg atgtatgcgc tgctttcagt cttctcgttg ctggatgtta ttttgaatct
    31681 ggcgcagaaa ttcccggcgg catctcagtt accgctgaaa gggatatttc aggggattaa
    31741 actgatcggc gcgatcctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc
    31801 agcgattctg atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga
    31861 tccgattctt ggtctggtgg ccggtattca gctttccgcg aacgatatgc tgaaactggg
    31921 cgactggctg gagatgccga aatacggcgc agatggcgcg gtgatcgata ttgggttaac
    31981 caccgtcaaa gtgcgtaact gggacaatac cattaccact attcccacct ggtctctggt
    32041 ttctgactcc tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg
    32101 cagtatcagt attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct
    32161 gaataaagcg catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg
    32221 gaatcgccag caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg
    32281 aacctttcgt gcctatctga acgaatatct gcgtaaccat ccgcggattc gtaaagatat
    32341 gaccttaatg gtacgccaac tggctccagg tgataacggt ttaccgctcg agatctacgc
    32401 gtttactaac accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgatca
    32461 catatttgcg attgtcgaag agtttggtct gcgacttcat cagtcgccaa ccggcaatga
    32521 tattcgctct ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg
    32581 atgagagcgg acatcgtttt gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt
    32641 ttaaatgcca caaacaggaa tacaatccac accggcagca ggatcgctga caagcgcata
    32701 tcatccatcg tgcacatcag cagcaaaatc atgccgagga aggcaatgca gagatagttt
    32761 ccgaacggat agagcagcgc cttaaactgt gtttcacgcc cctgacgtcg catcgctgca
    32821 cgaaaacgca gatgcgccag acagatcata atccagttca acagcagcgt tgctaccacc
    32881 agcgccatca gcagaccaaa cgctttttgc ggcagcagat agttgattaa caccaccagc
    32941 gaagtgatcg ctccggaaag catcagcgag ttaatcggca caccgcgacg gctgacgcga
    33001 gtcaaaaact tcggcgcatt accctgcaca gaaaggccaa acagcatgcg gctgttagag
    33061 taaaccccgc tgttatacac tgacagcgat gctaccagaa tgacgaagtt cagcgcagaa
    33121 gctaccacgt tgctgtcgag attatggaaa atcatcacaa acgggctact gttggatttc
    33181 acttccaccc acggatagag cgccagtaaa accaccagtg aaccgatgta aaacagcagg
    33241 atgcgataca ccacctgatt taccgctttt ggaatgcttt tatccggatc gcgcgcttca
    33301 gcggcagtaa tcccaatcag ctccagaccg ccgaaggaga acataattac cgccagcgac
    33361 aaaatcagcc cattccagcc ggtggcgaag aaaccaccgt agcgccagag gttgtcgata
    33421 ctggctttct cgccgccgtg accagaaaac agcagccaca ggccaaagcc gatcataccg
    33481 atgattgcca gcactttaat caacgcaaac cagaactcgg tttcgccata taagcgcacg
    33541 ttcaccaggt taacggcgtt gatgataata aagaaggcgg cagcccaaat ccacgttgga
    33601 acatccggga accagtactg catatagatg cccgcagcgg tcagctctgc cattcccacc
    33661 agcacgaaca ttacccagta gttccagcca gagaggaagc ccgcaaacgg tccccagtat
    33721 ttataggcaa agtgggcaaa tgaaccggat accggctctt cgaccaccat ctcgccaagc
    33781 tggcgcataa tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa tacagccgga
    33841 cccgccatct gaatcgccgg accaatgcca agaaacagac cagtaccaat tgcgccaccc
    33901 agcgcaatca gttgaatatg acggttatgt aatccgcgat gaagcgtcgg ctcttgattc
    33961 gacgcagtat cttccgatac ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt
    34021 ttgttgaggg gcacctttta acatttagtg cccatcgtcg caagacacaa tccacacggt
    34081 taaacggggt atcctgcttt tatttccgta cccgatgtcg gtgcagccac atcagcttat
    34141 acgccgccgg gataataaac agcgacagca aaggtgcggt gatcatgccg ccaatcatcg
    34201 gtgcggcaat ccggctcatc acctctgaac cagcccccgt tccccacaga atcggcagca
    34261 gaccggcgat aatcaccgcc accgtcatcg ctttcgggcg cacgcgcagt accgcgccgt
    34321 gatacaacgc ctcatccagc ttctgctcac tgaatgtttg cggattattc aacgacggct
    34381 cggcctctat ggcgtgacgt aaatacatca gcatcaccac gccaaattcg gcggcgaccc
    34441 cggcaagggc aataaaaccg gtgcccgttg ccacagaaag atgaaagccc atccaccaca
    34501 gcaaccagat gccgcccacc agcgcaaacg gtacgctgct gataatcagc aacgcttcgc
    34561 ccacccgacg gaatgccaga tacaacagca cgaagatgat catcagtgtc atcggcacca
    34621 tgagtttcag cttatggttg gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc
    34681 tggtgcccgg ttttaactgc actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg
    34741 acaccatgtc acgatcgcgg gcatcgatat aaatccagct cgtcgggcgc gcattctcgg
    34801 ttttcagcat cgacggtccg gtagagactt taacgtcggc cacgtctgcc agggtgattt
    34861 gctgcttcat cggcgtcagg atcggtagct ggcgcagtgc ctgcgggcta tcgcgccagc
    34921 tttgcggata acgcagatta atgggataac gggcaatccc ttccaccgtt tcgccaacca
    34981 tcgccccgcc caccgcagaa gtcacaaaca actgcacatc cgccaccgtc ataccgtaac
    35041 gcgcggcttt ttcacggtta atctcaacgt tgatatagcg cccaccttcc agacgctcgg
    35101 ctagagcaga agccacgctt ggcaccgttc gcgctacttc ttcaatttgc tcagccatcg
    35161 cgtcgatatc cgccagcaca gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg
    35221 ttgagagcat atcgatacgg ttacgaattg gcggcaccca cagattcgcc agccccggca
    35281 gccgcacggt gttatccagt tcctcaatga ttttgtccat cgtcatgcca ggccgccact
    35341 gatcctgcgg cttaagctgg atggtcgttt caaccatctc aagcggagca gaatcggtgg
    35401 cggtttccgc tttcccggtt ttgccaaata cccgcgccac ttcaggtacg ctcataatta
    35461 gcttgtcggt tttttgcagc atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg
    35521 atggcatata caacaagtcg ccttcattga tctgcggtaa aaattccccg ccaactttat
    35581 tgagcggcca gagaaccgtc agcaccgaaa gcgctgccac caacagcgtg gttttcggcc
    35641 agtgcagtac tttcagcaat agaggatgat aaacacgaat caaaaagcga ttgagcgggt
    35701 tactgctttc cggcggaatt ttgccacgga tccagtaccc catcaggata gggatcacta
    35761 cgatcgccag cagcgccgcg cccgccatcg cataggtttt agtgaacgcc aacgggccaa
    35821 acagacgccc ttcctgccct tccagggtga agatcgggat aaacgacaac gtgataatca
    35881 gcagactgat aaatagcgcc ggacccactt caacagatgc atcggtgatc acctgccagc
    35941 gcgttttatt atccagcgtg gcgtcaggat gctggtgctg ccactcttcc agccgtttat
    36001 gtgcattttc gatcatgacg atagcggcat cgaccatcgc cccgacggca atcgcaatgc
    36061 cgcccaacga cataatattg gcattcagtc cctggaagtg catgacaata aaagcaatac
    36121 acaaccccag cggcaacgaa ataatcgcca ccagcgccga gcgcacatgc cagagaaaca
    36181 gcgcacagac taccgccacc acgataaact cttccagcaa cttgccgctg aggttgtcga
    36241 tggcgcggtc aatgagctgg ctgcgatcgt atgttgtaac tatctccacg ccttccggca
    36301 gactactttt cagcgtttcc agtttgtcct tcacggaggc gatcacttct cgggcgtttt
    36361 tgccggagcg caggatcacc accccgcccg ccacttcgcc ttcgccattc agttcggcaa
    36421 tgccccggcg catctccggg ccaatctgga ccttcgcaac atcacgcaga taaacgggca
    36481 cgccattttc actggctttt aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc
    36541 cgctggcgcg caccatatat tccgcttccg ccagttcgat cgacgaaccg cccgcttcct
    36601 ggtttgaagc atccagcgcg ctttttactt cggctagact gatgccatat tgcgccaggc
    36661 gctggggatc gataaccacc tgatactctt tcaccacgcc gcccaccgac gccacttccg
    36721 caacgtcagg gatggttttt agctcatatt tgagaaacca gtcctgcaat gagcgtaaat
    36781 cggccagatc gtgcttaccg ctgcgatcca ccagtgcata ttcatagatc cagccaacac
    36841 ccgtggcatc tggtcccagc tcggcgctga ctcccgcagg cagcttaccc tgtacctggt
    36901 tgaggtactc cagcacccgc gaacgcgccc agtacggatc ggtaccatct tcgaaaatga
    36961 catacacata agagtctcca aactgcgaga aaccgcgcac agtcttcgcg ccaggcaccg
    37021 acaacatggt ggtggttagc ggataagtca cctgattttc aacgatttgc ggtgcctgac
    37081 cgggatagct ggttttaata atcacctgca catcggagag atccggcagc gcatccactg
    37141 gcgtgttaat gatggtccag gtgccccaga tgctcagaaa caacgcgccc atcagcacca
    37201 aaaaacggtt cgccaccgag cgacgaataa tccattcaat cattggttat tccctcaatg
    37261 cgcattggta gcactttcag agcgcatccg ctccagtgcg ccagaaatat tggcttcgga
    37321 atcaatcagg aacaggccgc tggaaaccac cttttcacct tccgccagac cagagtgtaa
    37381 cgcggtgacg ccttgcgatg cctggaaaac ggcaacgcgt ttcggtacaa agcgtccgtc
    37441 ggcatcaacg gtaatcaccc gctgttcgtt gccggtatca atcagcgcct gtgacggaat
    37501 gagcagcatc ggttcgctgg cggtgttgag ttgcaaccag gcgttcatgc ccggttttag
    37561 cgcctcgtcg gcgttgtcga cttccagacg cagctgcagc gtgcgggtcg cggcatccac
    37621 gccaggtagc agcgtccatt tgcggatggt gagtgtttta tccggtcgcg ccggaacggt
    37681 gagcgtaaac tgcgaggcat ctttcaccag ccaggcgata gactccggga tcgcagcagt
    37741 gacccacacc gggtccatac cctgaatttt cgctaccacg ttatctttgg cgatattcat
    37801 tcccgcgcgc agatcaaacg cggtgatcac gccatcaatg ggtgctttga gcgtaaagcg
    37861 agtctggatt ttttgcgtgg cgatcaggcg gcgaatatcc gcctccggca ttcccgccag
    37921 tcgcagccgc tcaagaatgc cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag
    37981 taaatactca ctctgcgctt ccacccagtc aggaatggtc aggtcgagaa gcggtgtgcc
    38041 cttttgtact ttatcgccca cggtaagcgg atacaccttg tcgataaacc cggcagcgcg
    38101 agcctgcaca atggcatact gatactcgtt gtaactgaca ttcgccggga aactctgggc
    38161 aaaagtcagc ggtccgcgcg tgacggtagc cgttttcacc cccagattct gcgtctgagt
    38221 cgggtcaatg cgcacaccag acgcagaact ctcttcatcg gcatatttcg gcaccagatc
    38281 catatccata aacggcgatt tacctggttt atcgaaccgc gtattggggt acatcgggtc
    38341 gtaccagaat aagattttac gttctgcggt cgacgttttt tctgcgggtg gttcagcctt
    38401 tgcaaaccag gtaaaacctg ccgcagaaat aataccgccc gcgatcatgc tgccgataat
    38461 aagcgcgatt tttttcatct cattaaacct gggttactgg ctgactttaa tatcctgtaa
    38521 taaagaaagg ttgccctgct ggacaaaatt aaacgccact ttgtcgccgg ttttaatttc
    38581 actcattttc gtctgcgggg tgatggtaaa gcgcatagtc atctccggcc agttcacggc
    38641 agcaatcgga tcgtgatgga tggtgatttt tttgctttcc agatcaatac cctttaccac
    38701 gccagtggcg ctaataacct gcggttgtac ttcgctcatg gtttcatgat gatgttcgtt
    38761 agcctgggca ttaaagccaa taacggtaaa cagactgaac attgcgactt gcagtgcttt
    38821 tttcatttat tctctcctgg agttaaaaat tattgctgcc aaccgccccc taaggcggta
    38881 tacaaagaaa tttcgttaac ctgacgggca taattcaggt cgagtaaagt ttgtcgggtt
    38941 gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc gtgctgatat
    39001 aatgcccgcg cccgttgcaa agtaatttgc agcgacgcca gataacgctg ctgggcgctg
    39061 atttgatcgt tcaggctttg acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc
    39121 tggattttct gttcataatt cactaccgac tgctgctggc gaatttcggc gatatccaga
    39181 ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat aaaattccac
    39241 atcccgctgc tagcgttaaa taatgaagac agatcgctgc tggcggttga tattccgctg
    39301 gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc attagccgcc
    39361 attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaag
    39421 cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc ttgcggcagt
    39481 ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag ttccccctga
    39541 cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc cagcgccaga
    39601 acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta
    39661 cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gttgctgatt gaaatagctt
    39721 tgcgcgacat tagaaaccag cagaatatgc accgcgcgct gagcttcctc agtggctaaa
    39781 taattttgtc gctcggcttc gctcatgttc tttaagcgac cgaaaaaatc gaggtcaaag
    39841 ctggcgttaa ggccggtcga aaactcccgt gtcgtggcgg tattcccttt aagattaccg
    39901 ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc ggtcagacga
    39961 tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg gttattcacc
    40021 agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccacg
    40081 ttctggtagt tatctgctgc gttaaccaag ccgttctggc tgagtgagaa ctgctgcggc
    40141 acgggcattg ccgtacgctg ataatccggt gccaatgaac aaccggttag cgcaagggcc
    40201 acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt gattttatac
    40261 cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca aaattgtcat
    40321 tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg
    40381 gaggaaaaat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca
    40441 aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc
    40501 atctggcgat gaccggtgat tatgacctga taatcctcga tattatgctg ccggacgtga
    40561 acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg attctgttgc
    40621 ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gccgatgact
    40681 atctggtgaa gccgttcgct tttgctgaac tgctggcgcg ggtgcgcact ctcctgcggc
    40741 gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatctgatg gtcgatctcg
    40801 tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa gagtttactc
    40861 tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc
    40921 aggtgtggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg gtgaagcggc
    40981 tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcagaccgtg cgcggcgtgg
    41041 gctatatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca
    41101 acccgtctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg
    41161 atcatgatcc actcagtgaa agtgcatttt gccgagcagg atattaatga tttaaaagaa
    41221 attagcgcca cccttgaacg ggtacttaat catcctgacg agacgcaagc ccgacgctta
    41281 atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct ggcagatagc
    41341 cacggcaaaa cggtgtatca ctcccccggt gcgccggata tccgcgagtt tacgcgtgac
    41401 gccatacccg ataaagacgc tcagggtggc gaggtgtatc tcctttccgg cccgacgatt
    41461 atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat gattaacttg
    41521 ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc gctttcgatc
    41581 gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac cgcatctgtc
    41641 atcagcatcc tgatcgtctt tatcgtgcta ttggcggtac ataaaggtca cgcgccgatc
    41701 cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt tcgccttgac
    41761 ccgcagaccg agcccattga gctggaacag ctggtactgt cgttcaacca tatgatcgag
    41821 cgtattgagg atgtctttac ccgccagtcc aatttctcag cggatatcgc tcacgaaatt
    41881 cgcacgccga ttaccaatct catcacgcaa acggaaatcg ccctcagcca gtctcgcagc
    41941 cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa
    42001 atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat ccccgaaaag
    42061 aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga ggcgttagcg
    42121 gaagatcgcg gcgtggagtt gcgatttgtt ggcgacaaat gtcaggttgc gggcgatccg
    42181 ctgatgctgc gtcgggcgtt aagcaacctg ctttctaatg ccctgcgtta tacgccaacc
    42241 agagagacaa ttgtagtgcg ctgtcagacg gtcgatcacc aggtgcaagt taccgtcgaa
    42301 aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctaccgc
    42361 gttgaccctt cccgccagcg aaaaggcgaa ggtagcggca ttgggctggc gatagtgaaa
    42421 tcgattgttg tcgcgcataa aggcacggtt gcagtaacgt cagatgcgcg ggggacaagg
    42481 tttgtgatta tgctgccgga gagagagtga ttattaagtt caaattcagg gcgtggtgca
    42541 tcacctcctg gtattgttga tcaacaaatt agagaaaaac ttattaaaca tatgaaatat
    42601 cgaaggctt
//
LOCUS       DAZPLM010000013        40686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPLM010000013 DAZPLM010000000
VERSION     DAZPLM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.013"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            229..1326
                     /gene="mltA"
                     /locus_tag="ACHVC7_000601"
     CDS             229..1326
                     /gene="mltA"
                     /locus_tag="ACHVC7_000601"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR2114737.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1403..2209
                     /gene="tcdA"
                     /locus_tag="ACHVC7_000602"
     CDS             1403..2209
                     /gene="tcdA"
                     /locus_tag="ACHVC7_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR2114738.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2260..2703)
                     /gene="csdE"
                     /locus_tag="ACHVC7_000603"
     CDS             complement(2260..2703)
                     /gene="csdE"
                     /locus_tag="ACHVC7_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR2114739.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2703..3908)
                     /gene="csdA"
                     /locus_tag="ACHVC7_000604"
     CDS             complement(2703..3908)
                     /gene="csdA"
                     /locus_tag="ACHVC7_000604"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR2114740.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4100..4327
                     /locus_tag="ACHVC7_000605"
     CDS             4100..4327
                     /locus_tag="ACHVC7_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR2114741.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4678..5595
                     /gene="gcvA"
                     /locus_tag="ACHVC7_000606"
     CDS             4678..5595
                     /gene="gcvA"
                     /locus_tag="ACHVC7_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR2114742.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5614..6009
                     /locus_tag="ACHVC7_000607"
     CDS             5614..6009
                     /locus_tag="ACHVC7_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR2114743.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            6002..7102
                     /gene="rlmM"
                     /locus_tag="ACHVC7_000608"
     CDS             6002..7102
                     /gene="rlmM"
                     /locus_tag="ACHVC7_000608"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR2114744.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7146..7877)
                     /gene="fucR"
                     /locus_tag="ACHVC7_000609"
     CDS             complement(7146..7877)
                     /gene="fucR"
                     /locus_tag="ACHVC7_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR2114745.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7935..8357)
                     /gene="fucU"
                     /locus_tag="ACHVC7_000610"
     CDS             complement(7935..8357)
                     /gene="fucU"
                     /locus_tag="ACHVC7_000610"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR2114746.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8359..9777)
                     /gene="fucK"
                     /locus_tag="ACHVC7_000611"
     CDS             complement(8359..9777)
                     /gene="fucK"
                     /locus_tag="ACHVC7_000611"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR2114747.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9886..11661)
                     /gene="fucI"
                     /locus_tag="ACHVC7_000612"
     CDS             complement(9886..11661)
                     /gene="fucI"
                     /locus_tag="ACHVC7_000612"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR2114748.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11694..13010)
                     /gene="fucP"
                     /locus_tag="ACHVC7_000613"
     CDS             complement(11694..13010)
                     /gene="fucP"
                     /locus_tag="ACHVC7_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR2114749.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13557..14204
                     /gene="fucA"
                     /locus_tag="ACHVC7_000614"
     CDS             13557..14204
                     /gene="fucA"
                     /locus_tag="ACHVC7_000614"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR2114750.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14232..15380
                     /gene="fucO"
                     /locus_tag="ACHVC7_000615"
     CDS             14232..15380
                     /gene="fucO"
                     /locus_tag="ACHVC7_000615"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR2114751.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15485..16240)
                     /gene="xni"
                     /locus_tag="ACHVC7_000616"
     CDS             complement(15485..16240)
                     /gene="xni"
                     /locus_tag="ACHVC7_000616"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR2114752.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16352..17719)
                     /gene="sdaB"
                     /locus_tag="ACHVC7_000617"
     CDS             complement(16352..17719)
                     /gene="sdaB"
                     /locus_tag="ACHVC7_000617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR2114753.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17777..19066)
                     /gene="sdaC"
                     /locus_tag="ACHVC7_000618"
     CDS             complement(17777..19066)
                     /gene="sdaC"
                     /locus_tag="ACHVC7_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR2114754.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19622..20986)
                     /gene="ppnN"
                     /locus_tag="ACHVC7_000619"
     CDS             complement(19622..20986)
                     /gene="ppnN"
                     /locus_tag="ACHVC7_000619"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR2114755.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21098..21946)
                     /gene="queF"
                     /locus_tag="ACHVC7_000620"
     CDS             complement(21098..21946)
                     /gene="queF"
                     /locus_tag="ACHVC7_000620"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR2114756.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22014..22559
                     /gene="syd"
                     /locus_tag="ACHVC7_000621"
     CDS             22014..22559
                     /gene="syd"
                     /locus_tag="ACHVC7_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR2114757.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23181..23510
                     /locus_tag="ACHVC7_000622"
     CDS             23181..23510
                     /locus_tag="ACHVC7_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR2114758.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23510..24292
                     /gene="truC"
                     /locus_tag="ACHVC7_000623"
     CDS             23510..24292
                     /gene="truC"
                     /locus_tag="ACHVC7_000623"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR2114759.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24310..24759
                     /locus_tag="ACHVC7_000624"
     CDS             24310..24759
                     /locus_tag="ACHVC7_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR2114760.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25194..26546
                     /gene="gudP"
                     /locus_tag="ACHVC7_000625"
     CDS             25194..26546
                     /gene="gudP"
                     /locus_tag="ACHVC7_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR2114761.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26548..27888
                     /locus_tag="ACHVC7_000626"
     CDS             26548..27888
                     /locus_tag="ACHVC7_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR2114762.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27909..29249
                     /gene="gudD"
                     /locus_tag="ACHVC7_000627"
     CDS             27909..29249
                     /gene="gudD"
                     /locus_tag="ACHVC7_000627"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR2114763.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29481..32237)
                     /gene="barA"
                     /locus_tag="ACHVC7_000628"
     CDS             complement(29481..32237)
                     /gene="barA"
                     /locus_tag="ACHVC7_000628"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR2114764.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32294..33595
                     /gene="rlmD"
                     /locus_tag="ACHVC7_000629"
     CDS             32294..33595
                     /gene="rlmD"
                     /locus_tag="ACHVC7_000629"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR2114765.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33643..35877
                     /gene="relA"
                     /locus_tag="ACHVC7_000630"
     CDS             33643..35877
                     /gene="relA"
                     /locus_tag="ACHVC7_000630"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR2114766.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35955..36203
                     /gene="mazE"
                     /locus_tag="ACHVC7_000631"
     CDS             35955..36203
                     /gene="mazE"
                     /locus_tag="ACHVC7_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR2114767.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36203..36538
                     /gene="mazF"
                     /locus_tag="ACHVC7_000632"
     CDS             36203..36538
                     /gene="mazF"
                     /locus_tag="ACHVC7_000632"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR2114768.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36609..37400
                     /gene="mazG"
                     /locus_tag="ACHVC7_000633"
     CDS             36609..37400
                     /gene="mazG"
                     /locus_tag="ACHVC7_000633"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR2114769.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37628..39265
                     /gene="pyrG"
                     /locus_tag="ACHVC7_000634"
     CDS             37628..39265
                     /gene="pyrG"
                     /locus_tag="ACHVC7_000634"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR2114770.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39353..40651
                     /gene="eno"
                     /locus_tag="ACHVC7_000635"
     CDS             39353..40651
                     /gene="eno"
                     /locus_tag="ACHVC7_000635"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR2114771.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10072 a         9854 c        10822 g         9938 t
ORIGIN      
        1 ccaggctgct ccaccccgcc tccgtggatg cgcactatac tccgctcgcg ttttcatgca
       61 accttttttt taccccgatc acggatctgg catgattttg aacaaaaaca aatcactaaa
      121 gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg
      181 gtttgttatc ttcgttgcgc cttatttttt aacctgaaga agagaacaat gaaaggacgt
      241 tgggtaaagt accttcttat gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa
      301 ccaaccgatc gcggacagca atataaagac gggaaattta cccagccttt ctctctggtg
      361 aaccagccag atgccgttgg cgcgccgatt aacgccggtg attttgccga gcaaattaac
      421 catatccgta attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg
      481 caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg cattgatgcc
      541 tggcagatgg aaggtgccga caactatggt aacgtgcagt ttacaggcta ttacacgccg
      601 gtaattcagg cgcgccatac ccgccagggc gagttccagt atcctattta ccgtatgccg
      661 ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg cgggggcatt gagtgataaa
      721 tatattctcg cttacagtaa ctccctgatg gataacttca ttatggatgt gcagggtagt
      781 gggtatatcg actttggtga tggcagtccg cttaactttt tcagctatgc agggaaaaac
      841 ggtcatgcct atcgcagcat tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa
      901 gatatgtcga tgcaggcgat tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc
      961 gagctgctgg aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg
     1021 aaaggggcaa gtgcggtgcc gctggttggt cgcgcgtcag ttgcctctga tcgttccatt
     1081 attccgccag gtactacctt gctggcagaa gtgccgttgc tggataataa cggcaaattt
     1141 aatggtcagt acgaactgcg tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc
     1201 caacacttcg atatctatca agggatcggg ccggaagccg gacaccgcgc aggttggtac
     1261 aaccactatg gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc
     1321 ggctgatgtg ttattctgta taccgttttt acggatacga cagggtgagg gataacctca
     1381 ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt
     1441 tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat
     1501 ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg
     1561 gattggcgca atcacgctta tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca
     1621 aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat
     1681 tcgccagatt aacccggagt gccgtgtaac ggtggtggat gatttcgtga cgccggataa
     1741 cgtagcgcag tatatgagcg tgggttattc gtacgtgatt gatgccattg atagtgtacg
     1801 gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg
     1861 tggcgcgggt gggcagattg acccgacgca gattcaggtt accgatctgg cgaaaacgat
     1921 tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa
     1981 aaacagtaaa ggtaagctcg gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc
     2041 gcagtcagac ggtactgtgt gtgcgatgaa agccacggca gaagggccga agcggatgga
     2101 ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt
     2161 ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc
     2221 ggatgagacg cgaacgtctt atccgacccg gttctatgct taaacctgct tcgtcgcagc
     2281 gataatcgcc tcgcttaacg catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg
     2341 caatcccagc tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt
     2401 cccctcaacg gcagtcaaca gcaccgccag caggccgcgc acaatgcgcc cttcgctatc
     2461 gccaaagaag tgcattttac cgttttcagt cacggtatat cccagccaga cgcggttttc
     2521 gcatccggca atctctttag cctgcgcttt taactcgtct ggcaaagccg gaagctgttt
     2581 ccccagcatg atcaactggc gatatttatc ttcccattgc gacaacggtg cgaaggtatt
     2641 gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt
     2701 cattaatcca ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc atccacatca
     2761 ctctttgtat tatatggcgc aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc
     2821 agtagcggct gagcgcaatg ttgcccggcc cgcagggcaa taccgtactc cgccagcagc
     2881 gtcaccatat cgctgtgatg aacgccagca aaatcaaagg ccagcaggct ggaatcctgg
     2941 cagcggaatg agcggaagcc aggacgtttt gccagcgcat cttccgccag cgtcgccagg
     3001 ctgcgactcc aactttctgc ctggttgata tcgtaatctg ccagccattc cagcgccgcg
     3061 cttaatccta tgacaccagc cacatttggc gttcctgctt ccagtttcca cggcgcagat
     3121 tgagtcgtga agccgtcaaa actcacttcg tgaatcattt tgccgccacc cagccagggc
     3181 gacatcgctt ccagcagttc tgatttgcca tacagcacgc cgatacccgt cgggccatac
     3241 agtttgtgac cagagaaagc atagaaatcg atatctagtt gctgaacatc tgcggggaaa
     3301 tgcaccgccc cctgagcacc atcaaccatc accaccatcc cggctgaatg agcaaaggta
     3361 atcgctcgcg ccagatccgg gcaaccgcca gtaacgttcg acatctgacc caacgccaga
     3421 atccgactac ggggagtaat cagttctggc aacaaatcga catccggcag tcgctgcgca
     3481 ttaagcggca atttcaccac tttggctcca gtttgttggg cgaccatcag ccaggggacg
     3541 aggttagcgt ggtgttctgc cacgctgaca ataatctcat cgcccggttg cagacgcgga
     3601 cgcgcatagc attgtgccac catgttgatg gattcagtgg tgccgcgcgt ccagacgata
     3661 gttttatcat ccggtgcatt cagtaattgc gccactttct ctcgtgcagc ttcataacgc
     3721 gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg
     3781 taaaactgtc gagtggcttc aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg
     3841 agatagacgc ccgcatcctg tagtgcggga aactgggcgc gaaactgcgc gggattaaaa
     3901 acgttcatgg tactcctcgg cttgatgcac cgatcgtgac gcaaaattca ctttgataca
     3961 aggagagtgc taaccatcag aattatctgg atatcctggc gaagccgcgt aagtaacata
     4021 tgctgaatag tgttaggcgt taattttagc ctgttaatca atactattaa gatttattaa
     4081 tcgctttaag gagaagatga tgaaaaagac tgccgcaatt atttctgcct gtatgctgac
     4141 ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg acggacgtac
     4201 catcgtctct gacggcaaac cacagactga taacgatacc ggtatgattt cgtataaaga
     4261 cgctaatggc aacaaacagc agatcaaccg tactgacgtg aaagagatgg tcgaactgga
     4321 tcagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat
     4381 cactatggac agacagggta aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg
     4441 tctgaatcgc agaccaattg caaacacaac aacacaacat cacaaccgta agccaaaagt
     4501 tcaccagaac acgcattccg ataaaacttt tcgttccggc tcaggaagtg ccgccactat
     4561 aggtatttgc tggtagaagc tcaacggaca atttataatg gctcagatta aaaaaactaa
     4621 taggttacac agtgtgatct aattgttaaa ttaatttaac atcaaagttt aaaagccatg
     4681 tctaaacgat taccaccgct aaatgcctta cgagtttttg atgccgcagc acgccattta
     4741 agtttcactc gcgcagcaga agagcttttc gtgacccagg ccgcagtaag tcatcaaatc
     4801 aagtctcttg aggatttttt ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg
     4861 ctgaccgagg aagggcaaag ctatttcctc gatatcaaag agatattttc gcaattaacc
     4921 gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg cgttgacggt cagtttactc
     4981 cccagtttcg ccattcattg gttggttccg cgactttcca gctttaattc agcttatccg
     5041 ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag ataagctggc ggatgatgtt
     5101 gatgtggcga tattttatgg tcggggcaac tggccggggc tacgggtgga aaaactgtac
     5161 gccgaatatt tattgccggt gtgttcgccg ctactgctga caggcgaaaa acccttgaag
     5221 accccggaag atctggctaa acatacgtta ttacatgatg cgtcacgccg tgactggcag
     5281 acatataccc gacagttggg gttaaatcat atcaacgttc agcaagggcc aatttttagt
     5341 catagcgcca tggtgctgca agcggctatt cacgggcagg gagtggcgct ggcaaataac
     5401 gtgatggcgc aatctgaaat cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg
     5461 gtcagtaaaa acgcttttta tctggtttgt catgacagcc aggcagaact gggtaaaata
     5521 gccgcctttc gccaatggat cctggcgaaa gccgctgctg aacaagaaaa attccgcttt
     5581 cgttatgaac aataatttac gtagggtacg accatgacca gccgttttat gctgattttc
     5641 gccgccatta gcggcttcat ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt
     5701 aaaaccatgg gggccgttga gatgggctgg atccagaccg gcctcgaata ccaggcgttt
     5761 catacgctgg cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag tatctggttt
     5821 tactggagta gcgttttcct cgcgttaggc acggtgttgt tcagcggcag cctttattgc
     5881 ctggcgctgt cccatctgcg tttgtgggca tttgtcacac cggttggcgg cgtgagcttc
     5941 ctcgcgggct gggcgttaat gttagttggt gctatccgtt taaagtgcaa gggcgtaagt
     6001 catgaataag gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat
     6061 taccgataaa gccggccagc gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg
     6121 ttatgtcatt tatgaatgtt atcaacctga tgatggcgat aagttaatcc gtgagctgcc
     6181 gttcagttca ttaatttttg cccgccagtg gtttgtggtg ggggaactct tgcagcattt
     6241 gccgccagaa gatcgtatta cccccattgt cgacatgttg cagggcgtag tagagaaggg
     6301 cggtgaactg cgtgttgaag ttgccgatac caacgaaagc aaagagttgc tgaaattctg
     6361 ccgtaaattc accgtgccgc tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta
     6421 tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt gcaccaggct gctgctatac
     6481 cggttactca tacagcaaca ataattcgcc gttctatatg ggcattccgc gcctgaaatt
     6541 tccggcagag gcgccgagtc gttccacgct caaactggaa gaggcatttc atgtgtttat
     6601 tcctgcggat gagtgggatg aacgcctggc gaacgggatg tgggcggtgg atttaggcgc
     6661 ttgccctggc ggctggacct accaactggt gaagcgcaac atgtgggttt attccgtcga
     6721 caacggcccg atggcgcaaa gtctgatgga taccgggcag gtgacgtggc tgcgggaaga
     6781 cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg atggtatgcg atatggttga
     6841 aaaaccggct aaagttgcgg cattgatggc gcagtggctg gttaatggct ggtgccgtga
     6901 aaccattttc aatctcaaac tgccgatgaa aaaacgctac gaagaagtat cacacaatct
     6961 ggcgtatatt caggcacagc ttgatgaaca tggcataaat gctcagattc aggcgcggca
     7021 gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg
     7081 tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt cgtcttcggc ctgctaccct
     7141 ttccgtcagg ctgttaccaa agaagttgca acctggcgct catcagaaat aatgtgcgtt
     7201 acctcatcca gatgcccgat gcgggcttcc cctgaacgat taaatttact tttatcaatc
     7261 aataacaacg attgcgcggc acgttttaat agcatcgatt tgtaatcagc gttgatcgca
     7321 ttggagtccc acagtgcgcc gctgctatcg atcccttcac aagaaaaaat aaacagatcg
     7381 atttccagcg atttaagttg ggaaatcagc gagggattga cgtaacagcc atatttgcgc
     7441 tcaagtgtgc cgccggaact gatcagttga atgcgttcgc gtttaccgag ttcatggcaa
     7501 atcggatggc tattggtgaa gacctgaatg ttgatgtcag gcaactggcg tgccagatac
     7561 cagcaagttg aactggcgtc taaggctatc accatccctt cttcaatcca cgcgagcgcc
     7621 tcgcgcgcga tatctgcttt atgcgcataa tggcttttca gcctgatgtg aaagggatcg
     7681 ccactgtctt gattttgacg gtggatatat ttagcgcgtc catgattgcg cagaatttta
     7741 ccctgcgtct gtaattcatt gagatcgcga cgaatggttt ctttactgac ctttagctgt
     7801 tcagagagag cttccgtggt caggctggta tggttcagca gcaggtcgac tatcgcttgc
     7861 tggcgtgccg ctttcatatt tttcacccca tcattcgata accgcccgcg tcatgcgggc
     7921 gtaccgatat gagattacgg tgttacccct ttttttaaaa gaatattccc gtacttcgct
     7981 cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga
     8041 ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt
     8101 tctacttcag gatcgagagt gtcaccttct accgctgcca tcatcaccag cggcggggca
     8161 taactgtcca gctcaaataa tgggataatc gcttggagca agtcgctcac caacaggcca
     8221 tcagcgcgga tcacctgcgg tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata
     8281 atttcatctc catgtcccat ctctgccagc actttcaata gttcgggaga aattaacggc
     8341 gaaattgttt tcagcatttc acacttcctc tataaattca ggttcagttt gcgggtagaa
     8401 ataacggtac tgataatgaa tctgtgcacg ggcttcttcc gggctgttaa attcccctac
     8461 gccataccaa ccgaacagcg cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt
     8521 taccggaata tcaagcatat tggctttaat ctgattccac aatgtgttgc gacttcctcc
     8581 accgactaac aataattcag aggctttaaa gtgcccgatt ttttccagca tctgtagatt
     8641 gcgctgtaat tgcgcagtta acccttccag cgccgcgcga tagaaatgcc cccgcgtggt
     8701 attaagcgtc actccttgcc agccagcgtt ctgacacgac aataaatcac actgcatttt
     8761 cacaccatcc gcgccaggtg cgatcagacg agcttcttca atcaacattt gccagggtgt
     8821 ttcagccgtc cagaacagtt ttctcaccca ttccagcacg ccggatgcca gccattgcat
     8881 acctgggtta tacaaccctg cctggctatc cagttcgcag gtagaatcgg cgtactgact
     8941 taacagcgaa gtatcaacct gggcgctacg gaccattaaa atttcccatg taccggaaga
     9001 gagcacgggt tcattttggt cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc
     9061 tgcggaaatc accggtatgc caacgggtaa gccgagcatt gctgcggcgc tgttctgtag
     9121 cgtaccaatc tgttcacccg cttccaccag acgagggaag agtcggcgtg gaataccggt
     9181 ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata tccagcatct ggctggttcc
     9241 ggccatcgtg atatcagtag tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat
     9301 aaagagccag gcgtgcgcgc gttccagcag ttgtggatga ttttctttca accacaccaa
     9361 cttatataac gtattgaaac taaaggctcc gacgccagaa atagcctgca accgctgtgc
     9421 ggagatcaac tgttcaatat tgtccataac cgctgctgtt cgcggacatt tccagctaat
     9481 gatcggatag agcagattgc cttgcttatc taccagagcg ccatccacac caaaggtggt
     9541 gacggcgata ccgcggatgt ggcattcagt cagttcacta ttgatttgcc gacagcaatc
     9601 agcaaagcgt tgcaaaatgg cgtctaaaga ccactggtgc caggtgttgt tttccatcgc
     9661 gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc
     9721 gatggccctg acattggtcg cgccacagtc gagtaccagg ataacttctt gtttcataat
     9781 cgctcctgca atatagccgg ataacattgc ttatccggct aaccactctt gcttgttcag
     9841 gcccggtagc cggagcgacc gggcatcaca tcagggagta atgtattaac gcttgtacaa
     9901 cggaccgtag ttctggcaag cgcggtaatc ctggccttca atatccatgc cgtgcgcagc
     9961 ccaggcagaa ggacgataca ctttggtctc ttcaacgttg tgcatacata ccgggatacg
    10021 cagcatggag gcgagagtga taaagtctgc gccaacgtgg ccgatggtca gaaccccatg
    10081 gttagcgccc cagttcgcca ttaccgagta cacatccgta aacggccctt taccggtgag
    10141 gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg cgtttgttga ggatgtcatg
    10201 cacatccttc ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc
    10261 tttgatgatg ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga
    10321 agagtaaccg ccgccacgga agtattcgtg gatcgccggg caccattcgg tagcggcgag
    10381 gcaagcgtca gcctcttgct gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc
    10441 gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa cctgagttga tcaaatggat
    10501 gatgccgtgt tctgccagtc catcgagttt atgccccgtt acacgctcaa ttgcttctgg
    10561 tgaccagtag gtacgcacat cggcaaatac ctgagcggtg ccggtgagct ggtgacccat
    10621 tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac
    10681 gccattccag tcaaatgaac tgttgaggat cgcttcggcg gtgtcaccat tgggatattg
    10741 atcggtccag tgacgttgcc cctggaagcc cgcagcgatg gcgttgtagc caagtgattc
    10801 ttccacgcga ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc ggatacacat
    10861 cgccatcagt aaactttcgc gcagaactgc gcggctttgc tcggcattgc gttgatactg
    10921 tttgttattt tcatcttcgc catagcggaa gtttttatca gcccaggtca gtgccatttc
    10981 cagttcggct tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg tcatatccac
    11041 cgcctggact ttcattccca gccaggattc aaagaagttg tgatcaacaa tagaaccggc
    11101 gatacccatc gaaacgccgc ccagcgacag atagctttta cctttcatgc tggcgacggc
    11161 caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc
    11221 atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg
    11281 agctgccaga gccgctgcca ggtaaacagc gccgggacgt tcagtgccgt taaagcccca
    11341 aatggctttc gggcgggttg gatccatgtc gatggtttca ctgccatagc accagcaagg
    11401 cgttaccgta atggtgaggc ctacattctg gctgctgaat ttttcttcgc aagcagcggc
    11461 ttcagccata cccgcgatac aggtatcgga aatgacacac tcgacggcag ctccgcaggc
    11521 atggcgcagt ttctcggtca gcagtgcggc cgtagctttc gccatattca ttgtttgttc
    11581 ttcaagcgac tcacgaacac ccatgcgacg accgtcaata accgggcgga taccaatttt
    11641 cggtaagctg atttttttca ttacagattc ctcactttat tcggaaaata tgttcagtta
    11701 gttgccgttt gagagcggaa acgggcaaag ataaagatga ccgcgaagca gagtgtgggg
    11761 atcagttcag cagtggggat gttgcccgcc gcgtcactga caaaacccat gaccggagtg
    11821 acaataccgc cgccaataat ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg
    11881 ccgagattct taatgcccag cgagaagatt gttgggtact gaatcgacat aaaggcgctg
    11941 cataaagtca gggctattaa gcccacatga ccgccagcga aggctgagat caggcacagt
    12001 gccatagcga ttaatgcgta ggcggccagg actttgtgtg gtgcgaagcg actgatgagc
    12061 caggtaccgg tgaaacgacc aataaagaag cacaccatgg ttccggttaa atagttagcg
    12121 gcaaagcctg cagtcatacc tagaatttct tctacagcgt agcgaatcaa atagctccag
    12181 caggccgttt gtgcgccaac gtagcagaat tgcgctaata ctgcccagcg ccagtggcga
    12241 atacgcgcta ggcgagaaag cgatgcggag aacgatcctt gtttggcgtc actgtgatta
    12301 tcactctgca atgccgggaa tttcgtcagc atgatcagca gggcgaccag taacacgata
    12361 gccacgatga tcatataagg tgtctgtacc gataatacca ggctgtgttt atacgcactc
    12421 aattgctctg gagacatttt atcgagaacg tcttgcgatt gatgtggcac gttagacaaa
    12481 ataagacttt gcccaaagac aaccgcgata attgcgccaa acgagttaaa tgtttgcgca
    12541 agatttaagc ggaagtgacc actgctttcc ggccctaata ccgtaacaaa agggtttgcg
    12601 gcagtttcca gacaacctaa tccggctgca ataataaata ggccaactaa aaataaggtg
    12661 tagttcatta tttctgcagc gggccagaat aatgcagcgc ccagggcata taaaaataac
    12721 ccagtaataa ttcctgcttt ataactgagt tttttcatca atatcccagc aggaattggg
    12781 ataatgaaat aaccaaagta aaaggccgat tggatcaggc cagcctggaa atttgtcagc
    12841 gtaaaagcct gctggaattg aggtaataaa atgtcgttaa ggttattggc taccgcccaa
    12901 agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt aacttctgct ttgccctgca
    12961 tctttatcta ccgcacggta actctgcgtt tgtattgatg tgtttcccat agcatcctct
    13021 taggttcaga agcttaagca cgtaatcggt tatgttttca aaagtgcaaa accagcgagt
    13081 tcgggcttca tatcagtgaa tatttgcctg tctgaaatga actcatggat tcattttcaa
    13141 ataaaaaacg ctctgtaata tgacggcggt cacacttatt agctggaagg tgactttatg
    13201 tgactaccat cactttaata atcgaaattt catgcccgtt tcagaaaata aatcataaaa
    13261 aacacaaacc agatgactgt attaattgtt tatgccgaaa acgggcattc ctggcttggg
    13321 tgaatagaaa aatgaccgtt ttgataaata tttttaagag cggtcatttt taatggggtt
    13381 ataaaaagag aacttgcagt tatttactgt gattagttga gctaggtcac aaaatataag
    13441 cttgtgaatt tctttcttgt taatcaggaa gaagatggtg ggtaattaaa cggctaattc
    13501 aatagtgtga aaggaacaac attattgccc tgttttgaat cagagagagg tagctaatgg
    13561 aacgaaataa acttgctcgt cagattattg acacttgcct ggaaatgacc cgcctgggac
    13621 tgaaccaggg gacagcgggg aacgtcagtg tacgttatca ggatgggatg ctgattacgc
    13681 ctacaggcat tccatatgaa aaactgacgg agtcgcatat tgtctttatt gatggcaacg
    13741 gtaaacatga ggaaggaaag ctcccctcaa gcgaatggcg tttccatatg gcggcctatc
    13801 aaagcagacc ggatgccaac gcggttgttc acaatcatgc cgttcattgc acagcagttt
    13861 ccattcttaa ccgaccgatc cccgctattc actacatgat tgcggcggca ggtggtaatt
    13921 ctattccttg cgcgccttat gcgacctttg gaacacgcga actttctgaa catgttgcgc
    13981 tggctctcaa aaatcgtaag gcaactttgt tacaacatca tgggcttatc gcttgtgagg
    14041 tgaatctgga aaaagcgtta tggctggcgc atgaagttga agtgctggcg caactttacg
    14101 tgacgaccct ggcgattacg gacccggtgc cagtgctgag cgatgaagag attgccgtag
    14161 tgctggagaa attcaaaacc tatgggttac ggattgaaga gtaatttcgt aaagcaacaa
    14221 ggagaaggat gatggctaac agaatgattc tgaacgaaac ggcatggttt ggtcggggtg
    14281 ctgttggggc tttaaccgat gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca
    14341 ccgataaaac gctggtgcaa tgcggcgtgg tggcgaaagt gaccgataag atggatgctg
    14401 cagggctggc atgggcgatt tacgacggcg tagtgcccaa cccaacaatt actgtcgtca
    14461 aagaagggct cggtgtattc cagaatagcg gcgcggatta cctgatcgct attggtggtg
    14521 gttctccaca ggatacttgt aaagcgattg gcattatcag caacaacccg gagtttgccg
    14581 atgtgcgtag cctggaaggg ctttccccga ccaataaacc cagtgtaccg attctggcaa
    14641 ttcctaccac agcaggtact gcggcagaag tgaccattaa ctacgtgatc actgacgaag
    14701 agaaacggcg caagtttgtt tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg
    14761 acgctgacat gatggatggt atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc
    14821 tcactcatgc tattgagggg tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc
    14881 acattaaagc gattgaaatc attgctgggg cgctgcgagg atcggttgct ggtgataagg
    14941 atgccggaga agaaatggcg ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg
    15001 ggttagggtt ggtgcatggt atggcgcatc cactgggcgc gttttacaac actccacacg
    15061 gtgttgcgaa cgccatcctg ttaccgcatg tcatgcgcta taacgctgac tttaccggtg
    15121 agaagtaccg cgatatcgcg cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag
    15181 aggcgcgtaa tgccgctgtt gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc
    15241 cacatttgcg tgatgttggt gtacgcaagg aagacattcc ggcactggcg caggcggcac
    15301 tggatgatgt ttgtaccggt ggcaacccgc gtgaagcaac gcttgaggat attgtagagc
    15361 tttaccatac cgcctggtaa atgcgctgat gtgataatgc cggatacgac gctagccgcg
    15421 tcttatccgg tatataagtg cattaaattc aatgtgttga taatcgtttg catccggctc
    15481 gccgttaccg taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta
    15541 agcgggcaat atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc
    15601 acttttcggc aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca
    15661 gcagctgcgt ggcgcttttt ggtccgattc ccgcaacacc cggtaccttt gaactgctga
    15721 tccccgcaag tccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga
    15781 taaatggcgc atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa
    15841 gtaactgaca gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca
    15901 ctttgaccgc cagcgtggcg gctaagtcat cggcttcgtt gccgctggtt gaccagcacg
    15961 ggacgccgcg ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg
    16021 gcattggcgg tcgacccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc
    16081 ggttttcatc atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat
    16141 cgagcgcatg ctggcaggtt tcgacacagg gcgatccctg aacggcatga atgcgacgaa
    16201 taagattcag tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca
    16261 ggcgataagg gtaacattcg atgccggaac aggctacggc tggatgagaa atcgggaaga
    16321 ggcctcgcaa aacgaggcct ctggagagcg attaatcgca ggcaacgatc ttcattgcca
    16381 ggccgccgcg agaggtttcg cggtacttgg cgttcatatc tttacctgtt tcgtacatgg
    16441 tttcgataac tttatcgagg cagacgcgcg gctcgctggt acggcgtagc gccatacgtg
    16501 cggcgttcac cgcttttact gccgcaatgg cgttacgctc gatgcatggc acctggacct
    16561 gtccggcgac cgggtcacac gtcagaccga ggttgtgctc catggcgatt tccgccgcga
    16621 tgcacacctg cgccgggctt gcacctaaca gttctgccag accagccgcc gccattgagc
    16681 acgccacacc aacttcaccc tggcagccga cttcagcacc agaaattgac gcgttcatct
    16741 tataaagaga accaatggca ctggctacca gcaggtaacg agccagtgag ttagcgttca
    16801 cttcgcggat gaacttgtcg tagtacgcca gcactgccgg gataatcccg cacgcaccgt
    16861 tagtcggcgc agtcaccacg cgtccgccag cagcgttctc ttcgttcact gccagtgcaa
    16921 acatgttgat ccagtcaaca accgccatcg ggtcagtggt ggttttatcc tggctgacca
    16981 gcatccggcg tagtgccgca gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt
    17041 cggtggaaat accgcgctca ataccgccac gcatgacttc ccatacgttc gccaggtgct
    17101 gttccagctc ttctttgctg tgcagcgcca gctcattttt catcatcagg ccagagagtg
    17161 acagcccggt ttcctgacaa tgtttttgca gatcggctgc tgaactgtac ggataaggaa
    17221 cttcaaccgg tgcgctattc tgctggccaa aatgctcttc atcaacgata aagccaccgc
    17281 caatagagta gtaagtctgg ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca
    17341 taccgttttc atgcagagaa aggttgtctg catggaagtt catgcactga tcaaccggga
    17401 actccacttc atgctgaccg tttgccagca tcaggcgacc atgagtattc acatcctgaa
    17461 taaaaccagg gatggaatcg atatccacgg tatccggcag gttacccgcc aggcccataa
    17521 taatggcgat atcagtgtgg tggcctttac cggtcagaga gagcgagccg tacacgtcaa
    17581 ccaccacgcg ggtcacgtct ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt
    17641 tacccgcttt cattggtcca acggtatgag aactggaagg gccaatgccg attttgaaaa
    17701 tatcgaatac gctaatcata ggaaatacat cgcgttaaaa cggaggaagc gccgcccgaa
    17761 agcggcgcga aaggacttag ctgaacagag agtagaagat tgcggagatt gcaatcagac
    17821 ccatcacgac aacgaatacg ttgctgatgt gaccgctgta cttacgcatt gccggtactt
    17881 tctgaattgc gtacatcggc atcaggaaca ggatcatcgc gatgattggg ccgcccaggg
    17941 tttcaatcat acccaggatg ctcgggttca gggtggcaac aatccaggtc gttaccagca
    18001 tgaacagcgc agtgatacgg ttcagcttgt tgatttcgat agacttacct ttaccacgca
    18061 gagatttaat caccataccg ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg
    18121 atttggtgat agcgataatc gcgataatcg gagccatcca cgcgataacc ggtgcgttaa
    18181 agtggttagc caggtaagac agaatcgaga tgttctgctc tttagccgca gccaggtctg
    18241 ccggagtcag gctcagtaca cagctgaaga cgaagaacat tacggtcagc accatcatga
    18301 tgtgtgcgaa tgccaggatc ttcgagcatt tctgttctgc catatcgccg tactcttcac
    18361 gcttcgcaac ggcgaaagaa gagatgatcg gagagtggtt gaaagagaac accataaccg
    18421 gaattgccag ccacagggtc atccacagac cgtttccggt tgcagatgca gtgtccagag
    18481 acagcgtttc cagtgctgcg ccgttccact gcgggatcag gtacagagcc agcagcatca
    18541 gtacgccaac aaacgggaat accagaatac tcattgcttt aacgatcatc tgctcaccga
    18601 agcgaacgat ggtcatcata cccacgatca ggatcagcga cagaatcgca cgcggcggtg
    18661 gtgtcatacc cagctggtga gacatgaagc tttcaactgt attggtgatt gccacgctat
    18721 aaaccagcag gatcgggtag atagcgaaga agtagagcag ggtaatcagt ttacctgcgc
    18781 caataccaaa gtgttcttct acaacctcgg tgatgtcttc gcctgggttt ttaccagaca
    18841 gtacgaagcg agtcaggccg cggtgagcaa aaaatgtcat cgggaacgca aggatagcca
    18901 tgatgatcag cgggatcata ccgccaacac cggcgttgat tggcaggaac agcacgcccg
    18961 cgccgattgc cgtgccgtaa aggcccagca tccacatggt gtctgtcttg cgccaggcac
    19021 tacgagagtc tttcgacgca atcgtgctgg tttgagtcgt ttccatctat ttctcctgga
    19081 ggaatgcaaa taattcagct tttagccagt tcagctcggg aaggtgcctg accgtaaaaa
    19141 tgaaatctgt ctatcggcga tggcttaatc atttaccgcc gtaataattt gagggcggaa
    19201 agatacacac aagttatgta tctatcagtg atcttgatcc caaatacaat aatctacttg
    19261 gaagatagat tatcccaacg ctttactctg taaatcaaaa gtaaaagaga atttaagggc
    19321 aatatttgcc cattaaacga catttgactt cttccggggc gcgcaatata tgtaggttga
    19381 actgaaaaaa tcaatacttt tagttaatca tggcgataat ttctttttca tagcgataaa
    19441 atgtggttta tgaaaatagg gaaacgtttg cgttacaaca taaattgtat gacatattaa
    19501 ctgcaatgat atttatcgcg tgagggtggt aagaaacatt catataacaa atgaaataaa
    19561 ttgatgggta tgtgaccaaa aacgagaaaa cccggcaaca aacgtcaccg ggggagggag
    19621 attacgtgca gatttcgtag caagggatgt aggctgagcc tggcaacttc atacgatgct
    19681 gggcaacaaa accctgtagc aggtcgtcca tacgacgcat aatctcttta tcgccgttga
    19741 ttttgtaagg accaaactct tcaatggcgc gaataccgac ttcttttacg ttacccgcca
    19801 caataccgga gaacgcacgg cgcagatcgg cagccagcac ttcaacaggt tgatccgggt
    19861 aaagcttcag attagccata ttctcgtgag acggctcaaa cggcatttgc aaatctggcg
    19921 caatgcgcat tgaccagtta aagctgtagg catcgcctgt gtcgcggcga ttttctttca
    19981 ccagcggcat cgattttttc atctgacgag cgacttcagc ggcgtcatca atgatgatgc
    20041 ggtaatggcg gcgcgcgttt tcacccaacg tatgcacgac aaactcgtcc agtacgcgga
    20101 agtagtcggc gctctctttc gggccggtga ggaacaatgg taaaacctga tctttgttgg
    20161 ccgggttcat taaaattccc agcaaataca gcaactcttc tgccgtaccc acaccgccag
    20221 ggaagataat gatgccgtga gcgatacgga caaacgcttc cagacgtttt tcgatatctg
    20281 gcatgatgat caattcgttg accagcgggt taggcggttc agcggcgata atcgacggct
    20341 ctgtcatacc aataaaacga ctgtctttgt aacgctgctg tgcgtgtccg accgcagcac
    20401 ctttcatcgg cgcttccatc gctcccggac cacagccggt gcagatattc agctcacgca
    20461 ggcccagctg attcccgacg cgacgggcat acaaatactc gttttcgtta attgagtgtc
    20521 cgccccagca gaccaccata tttggcgctt caccgacatg cagtgcgcga gcgttacgca
    20581 agatggaaaa gaccaggtta gtgatgtgga cggagttgtc gagattcagg ttgggaaaac
    20641 gaacggtgtt atggatttgc ccgtaaacga agagaatgtc acgcagaacc gcgaacaagt
    20701 tggcctgcaa cgcgcgaata attcgcccat cgacaaaagc ctcttccggg ggattaatca
    20761 gttccagctt tacgccgcgt tcacggcgca ggacgttaat atcgaaattt tcaaaacgag
    20821 acagcaattc tttgctgtta tcggtcaaac taccggagtt cagtacggca agtgaacagt
    20881 tgcgaaacag ttgatagagg tcgctgctgg cggtgcgttt aagcatatcc acttccagct
    20941 gcgacaacat atccatggag ccaagcgggc taatatgtgt aatcaagaaa aactccttat
    21001 gggacgaata tcgtcattcc ctttgattac aatagccctg cctgatgtcc tttcacaacc
    21061 ttctgcgtga atccagcacg caaaattgag aaaaaattta ttgccgaacc agtcttgtgg
    21121 tcgatgggac aaaatcgcta ttactgcgcc acgggttaat gtccagaccg ccacgacggg
    21181 tataacgtgc gtaaacgctc aatttttctg gctggcagaa gcgtaacagg tcattaaaga
    21241 tgcgttccac gcactgttcg tggaactcgt tgtgatgacg gaatgagacc aggtaacgca
    21301 gcagtttttc tctgtcaatt tggcgtccac gatactgaat ttggatcgaa ccccaatctg
    21361 gttgatgggt gatcaggcag tttgatttca gcaggtggct gacaagcgtc tcttccacta
    21421 ctttttcacc acaggtggca ttctccagat agtcagtagt gaattcatag ttatcgatag
    21481 tgatatcctg gtcatcaatg caagtgccat taaaatgacc tatcggctgg ccttccagtt
    21541 catcaagacg atataacgcc acgctaatct taccctgagc gcaagtgctt aagtcgcgct
    21601 ccagcgtctg gcgcacctca tcccagttat taaaacgcgt ctggttaaaa ctgttgagat
    21661 agagcttaaa actcttcgac tcaatcagat ttacgctggt gtaatcaagt tcaacatgac
    21721 cgacagcgac ctgcggcaaa cctttcgcat tcagccagga aagttcatac agcgtccaga
    21781 tatccgtacc gtgaaaaggc aggttatccg ctttcagacc cagcgggtcg cgattcaggc
    21841 tgcgtggaac gccttgcagt aggctggcgt cataggtatc ccggtaatcg gttgattttc
    21901 caagagtcag gcccgcaagt gcctgatggt ttgcataaga agacatgttt caccgtcata
    21961 aaccagataa actaaacgac aagtgtatct ggtttaagtg aagagagaaa ttagtggacg
    22021 atttgaccgc acaagccctg aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag
    22081 agcataaaag ctggccgtta agtgaggaac tgtacggtgt tccttcaccg tgcattattt
    22141 caactaccga agatgccgta tactggcaac cccagccgtt taccggggaa caaaatgtaa
    22201 acgcggttga acgcgctttt gatattgtga tacaacccac aattcatacc ttttatacga
    22261 ctcagtttgc cggggatatg cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa
    22321 cctggagtga agacgacttc cgtcgggtgc aggaaaatct gattggtcat ctggtgactc
    22381 agaaaaggct aaaactgccg cccacgctat ttatcgcgac gctggaagaa gagttagagg
    22441 tcatctcggt gtgtaatctc tccggtgaag tgtgcaaaga gacgctggga acacgtaaac
    22501 ggacgcattt agcctcaaat cttgcggaat tccttaatca acttaagcct cttctgtaat
    22561 ccaaataccc catctggttg tgagagatct cttacagacc ctgtaggaga tcgccaggaa
    22621 ataagcgaat acttaaaaag ataagaatcg ttattttcat ttaaaatcaa aatgttgatt
    22681 gttaattctt aactttcata tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa
    22741 gacttcgcga aaaagacgat tctatcttcg tcgacaggga gtcagacaac gaagtgaaca
    22801 tcaggatgat gacacttctg caggacacac caggatggtg tttcagggaa aggcttctgg
    22861 atgaagcgaa gaggatgacg caggacgcgt taaaggacac ctccaggatg gagaatgaga
    22921 accggtcagg atgattcggt gggtcaggaa ggccagggac acttcaggat gaagtatcac
    22981 atcggggtgg tgtgagcagg aagcaatagt tcaggatgaa cgattggccg caaggccaga
    23041 ggaaaagttg tcaaggatga gcagggagca acaaaagtag ctggaatgct gcgaaacgaa
    23101 ccgggagcac tgtgaataca gtgctccctt tttttattcc tgctatcctt cgcggcagtt
    23161 tttcttattg aagttgcttt atgaccactc atgaccgcgt tcgtttccag ttgcagacgc
    23221 ttgaagcgtt actgcgtgaa catcagcact ggcgaaacga tgaaccccag ccgcatcaat
    23281 ttaatagtac ccaaccgttc tttatggaca ccatggaacc actggagtgg ttgcagtggg
    23341 tgctgatccc gcgaatgcac gatttgttaa ataacaacca gccgttaccg ggcgcttttg
    23401 ctgttgcacc ctattatgaa atggcgctgg cgacggacca tccgcaacgc gcgcttattc
    23461 tggctgagtt ggaaaaactg gatgctcttt ttgcggatga tgcgagctaa tgctggaaat
    23521 actctatcag gatgaatggc tggttgcggt aaataaaccc tccggctggc tggttcaccg
    23581 tagctggctg gatcgcgacg agaaagtagt ggtcatgcaa accgtgcgtg accagatagg
    23641 acagcatgtt tttactgctc atcgtctgga ccgacccact tctggtgtgt tgttgatggg
    23701 attatccagc gaggccggac ggctgctggc acaacagttt gaacagcacc aaatccagaa
    23761 acgttaccat gcgattgtgc gcggctggtt gatggaagaa gcggtgctgg attatccact
    23821 ggtggaagaa ctggacaaaa tcgctgataa atttgcccgc gaagataaag gcccgcagcc
    23881 agcagtgacg cattatcgcg gtctggcgac cgtagaaatg ccggtagcga ccggacgtta
    23941 cccgaccacg cgctacggcc tggtggaact ggagccgaaa accggacgca aacatcagct
    24001 tcgccgacat cttgcccatt tgcgtcatcc gattattggc gatagcaaac atggcgattt
    24061 acgccagaat cgcagcggtg ctgagcattt tggcctccag cggttaatgc tacatgccag
    24121 tcagttgtca ttgacgcatc cttttactgg cgagccgctg actattcacg cgggcctgga
    24181 cgacacctgg atgcaggcgt tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga
    24241 aagggttgag ttcagtgcgc catcgggtca ggatggagag ataagttcct aatccaggga
    24301 gaaaacggta tggcggaaat tggtattttt gtcggcacca tgtacggaaa ttcgctgtta
    24361 gtggcggaag aagcagaagc aattctgacc gcacagggcc acaaagcaac ggtatttgaa
    24421 gatcctgaat taagcgactg gctgccttat caggataagt atgttctggt ggttacgtcc
    24481 acgaccgggc agggcgacct tcctgatagc attgtgccgc tctttcaggg aatcaaagat
    24541 agtctgggct tccagccgaa tctgcgttat ggcgtgattg cgctcggcga cagcagttat
    24601 gtaaatttct gcaatggcgg caaacagttc gatgccttgt tgcaggaaca gagcgctcag
    24661 cgggttggcg aaatgctgtt gattgacgcc agtgagaacc cggaaccgga aacggaatca
    24721 aatccgtggg tcgaacagtg gggcacgctg ttgtcctgat atgttcagcg agcggtaaat
    24781 gtcgttttag cggtgctgaa tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat
    24841 agcgcggatt tttactggcg tttgcctgga gtcaagcgat ccatttcata ctcttcttta
    24901 tttcttcgtt ttaacccttc ctttcttgtt cttgttttca tttccgtgaa gtggattcca
    24961 ccgtccaggg ctaatgccaa aatcgggcct cattgaacgc attaatgttg tgttgttgca
    25021 cggtgagccg ctatggcgcg ctttttatac tgctattgcc agatataaac acgcgccgta
    25081 ttcggcgaac gacctataaa aacggcaaaa aacaccctac gtcacctctg atttcctggc
    25141 tatgtcgcag tccagagtga gcgtggctaa cgcgaatttt caggagtgca acaatgagtt
    25201 ctttaagtca ggctgcgagc agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg
    25261 tgatgttgtt tatcgtcaca tccttcaact acggcgaccg cgctacgctt tctattgccg
    25321 gttcggaaat ggccaaagat atcggccttg atcccgtggg aatgggctat gtgttctctg
    25381 ccttctcatg ggcttatgtt atcgggcaga tccctggtgg ttggttgctg gatcgttttg
    25441 gttcaaaacg cgtctacttc tggtcgatct ttatctggtc gatgtttacc ttgctgcaag
    25501 gcttcgtcga tatctttagt ggattcggca ttatcgttgc cctgtttact ctgcgcttcc
    25561 tggtcgggct tgctgaagcg ccatctttcc ccggcaacag tcgcattgtt gcggcctggt
    25621 ttccggcgca ggaaagggga acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa
    25681 cggtgatctt cgcgccgatt atgggctggc tgacgcatga agtgggctgg tcacacgtct
    25741 tcttctttat gggcggtctg gggattgtca tcagctttat ctggttgaaa gtcatccacg
    25801 agccaaatca acatccgggg gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg
    25861 cgctgatcaa tatggatcag caaaacacca aagttaaagt gccgttcagc gtgaagtggg
    25921 ggcagatcaa acaactgctc gggtcacgga tgatgatcgg cgtttatatc ggtcagtact
    25981 gtatcaacgc cctgacttac tttttcatta cctggtttcc ggtttatctg gtgcaggcgc
    26041 gtgggatgtc gattctgaaa gcgggctttg tggcttccgt tccggcggtt tgcggtttta
    26101 tcggcggtgt gctgggtggg attatttccg actggctgat gcgtcgcacg ggatcgctga
    26161 acattgcgcg taaaacaccg atcgtaatgg gcatgttgct gtcgatggtg atggtgttct
    26221 gcaactacgt caacgttgag tggatgatca tcggctttat ggcgctggcc ttcttcggta
    26281 agggcatcgg ggcgctgggt tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg
    26341 gtctttccgg tggcctgttc aacatgttcg gtaacatttc tggcatcgtc acgccaatcg
    26401 caattggtta tatcgttggc acgactggct cctttaatgg ggcgctgatt tatgttggtg
    26461 ttcatgcctt aatcgcggta ctgagctacc tggtgctggt gggcgatatc aagcgtatcg
    26521 agctgaaacc tgtcgcgggg caataagatg acgacacaat ccagtcctgt tattactgat
    26581 atgaaagtca ttccggtcgc cgggcacgat agcatgttgc ttaatattgg tggggcacat
    26641 aacgcatatt tcacccgcaa tattgtggta ctcaccgata acgccgggca taccggcatt
    26701 ggtgaagcgc cgggcggaga ggtgatttat caaacactgg tcgatgctat tccgatggtt
    26761 ctgggccagg aagttgcgcg cctgaataaa gtggttcagc aggtgcataa aggtaatcag
    26821 gcagccgatt ttgatacctt cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc
    26881 gtggcggcgc tggaagccgc cttgcttgac ctgctaggta aggcgctgaa tgttccggtc
    26941 tgcgaactgt tggggccagg caagcaacgc gagactatta ccgtcctcgg ttatctgttt
    27001 tatatcggtg atcggaccaa aaccgatctt ccttatctgg aaaatacgcc gggcaaccat
    27061 gagtggtatc agttgcgcca tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg
    27121 gaagcctcac aggatcgcta tggctttaaa gatttcaaac ttaagggcgg ggtgttacct
    27181 ggcgagcaag aaatcgacac tgttcgtgca ttgaagaaac gcttccctga tgcgcggatt
    27241 accgttgatc ccaacggtgc ctggctactt gatgaagcca tttctctatg caaagggctg
    27301 aatgatgttc ttacctatgc cgaagatcca tgcggcgcag aacagggttt ctccggtcgt
    27361 gaagttatgg cggagtttcg gcgggcgacc ggcttgcccg tcgcgactaa catgatcgcc
    27421 accaactggc gcgaaatggg tcatgcggtg atgctcaatg cggtagatat tccacttgcc
    27481 gatccgcact tctggacgct ttccggtgca gtccgtgtgg cgcagctttg cgacgactgg
    27541 gggctgacct ggggctgcca ttctaataac catttcgata tctctctggc gatgtttacc
    27601 catgtgggcg cggcggcacc gggtaatcct accgctatcg atacccactg gatttggcag
    27661 gagggcgatt gtcgcctgac caaaaatccg ctggagatta aaaacggaaa aattgccgtt
    27721 cctgatgcgc ccggtctggg cgtggaactg gactgggaac aggtacaaaa ggcacatgag
    27781 gcctataaac gtctgcctgg cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc
    27841 cccggctgga cctttgaccg taaacgtccc gttttcggcc gtcattgatt ctgaaaaagg
    27901 acataaatat gagttctcaa tttacgacgt ctgttgttac tgaaatgcag gtcatcccgg
    27961 tggcgggtca tgacagtatg ctgatgaatc tgagtggggc acacgcaccg ttctttacgc
    28021 gtaatattgt gattatcaaa gataattccg gtcacactgg cgtaggggaa attcccggcg
    28081 gcgagaaaat ccgtaaaacg ctggaagatg cgattccgct ggtggtaggt aaaacgctgg
    28141 gtgaatacaa aaacgttctg acgctggtgc gtaatacttt tgccgatcgt gatgctggtg
    28201 ggcgcggttt gcagacattt gatttgcgta ccactattca tgtagttacc gggatagaag
    28261 cggcaatgct ggatctgctg gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg
    28321 atggtcaaca gcgtagcgaa gtcgaaatgc tcggttatct gttcttcgtc ggtaatcgca
    28381 aagccacacc tctgccgtat caaagccagc cggatgactc atgcgactgg tatcgcctgc
    28441 gtcatgaaga agcgatgacg ccggatgcgg tggtgcgcct agcggaagcg gcgtatgaaa
    28501 aatatggctt caacgatttc aaactgaaag gcggtgtact ggccggggaa gaagaagccg
    28561 agtctattgt ggcactggcg caacgcttcc cgcaggcgcg tattacgctc gatcctaacg
    28621 gtgcctggtc gctgaacgaa gcaattaaaa ttggtaaata cctgaaaggt tcgctggctt
    28681 atgcagaaga tccgtatggt gcggagcaag gtttctccgg gcgtgaagtg atggcagagt
    28741 tccgtcgcgc gactggcctg ccgactgcaa ccaatatgat cgccaccgac tggcggcaga
    28801 tgggccatac gctctcgtta caatccgttg atatcccgct ggcggatccg catttctgga
    28861 ctatgcaagg ttcggtacgt gtggcgcaaa tgtgccatga atttggcctg acctggggtt
    28921 cacactcaaa caaccacttc gatatttccc tggcgatgtt tacccatgtt gccgccgctg
    28981 caccgggtaa aatcaccgcg attgatacgc actggatttg gcaggaaggc aatcagcgct
    29041 tgaccaaaga accgtttgag atcaaaggcg gtctggttca ggtgccggaa aaaccggggc
    29101 tgggtgtaga aatcgatatg gatcaagtga tgaaagccca tgagctgtat cagaaacacg
    29161 ggcttggcgc gcgtgacgat gcgatgggaa tgcagtatct gattcctggc tggacgttcg
    29221 ataacaagcg cccgtgcatg gtgcgttaag acagaaaacc aaaaccggct ctatctggag
    29281 ccggcttgag actgatgcta aacgcaaaat agcctgttgt gctatgtatc aggtctacat
    29341 cattttttaa ttcattgaat ttaattggtt ttgtaggccg gataaggcgt taacgccgca
    29401 tccggcataa acacaggcac tttgtcacca atctgaaacc agcgtcataa aaagccgatt
    29461 gctactcgac aagacgtccg ttacccgaga attttgctgg cttcgcgcgc gacattatcc
    29521 atctcgtcca acagttccag cagctccggc tccaaatctt cttctttagt accgctacgt
    29581 agctgttgtt cgatcagttg gcagagattc ttcatacgcg gcacaccgct atagccgcaa
    29641 ctgccatgca gtttatgaat caaatcaacc aggccttccg ggttttctcc aaccagctgc
    29701 tcctcaactt tgttgcgaac ttcaggcagg aaatcgagta acatttgcag catatcgcgc
    29761 gctagatcgg tttttcctgc tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc
    29821 gggttcacca caatttcgtt gacttcgggc gtcacgacgc gagaggaaat accgctgcca
    29881 ggcttgtagc gcaacaacaa attatgcaat cgctcttctt caatcggttt cgccagataa
    29941 tcgctcatcc ctgcgccaag cagcttctct ttttgcccgg ccattgcatg cgccgttacc
    30001 gcgataaccg gcgtttgttg ctgatggggg agctggtgga tgagctcgca ggcccgaatg
    30061 ccatccatgt caggcatttg aatatccatt aagatcaaat cgaacggcat ctgtttcgcc
    30121 cgttcaaccg cctgatgccc gctatcgcaa agttccacat gttgcaccat atcttccagc
    30181 aatgcgccga taagtttcag gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc
    30241 agcttgcttt catcggttac aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc
    30301 agggcaggca acaggcgcgt aggtgttaat ggtttcagca gacacgcgcc gataccatct
    30361 tgcttgagtt tttctgcatt gacttgtgca tggcaaggaa gtgccagcat caggaaatcg
    30421 gtcatcgata ccgctttcgc taatcgctca tgttgcattg ttagcggctc gcggaaggtc
    30481 accgcgatgc ctaacaacat catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg
    30541 ctataaacca cttccagcgg cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg
    30601 gagtttggtt cgacataggc caggcgctta ccggcgaggc actgggtgga tggcccttcg
    30661 ataataatgt tcgggttcag atcgagatta atgtggaacc agaaagttga accgcgattc
    30721 ggctggctat ggaacgaaat atcgccgccc atttcattaa ccagtttttg tgtaatcacc
    30781 agccccagac cggtgccacc atgacggcgg gaaatactgg catcagcctg tcggaaggcc
    30841 tggaataagc gcgattgatc gcgttcagga ataccaatgc cggtatcccg aatctgcact
    30901 tcaatctgca ctttggtatt actcagcgca cgtttttcta ccagaatatc aatgttgcca
    30961 ttctcggtga atttaattgc attccccacc aggttagtga tgatttgctg taatcgcagt
    31021 gggtcgccga tcacgttatc aggcacgtcg cttttaatat tgagcgtcag ttccaaccct
    31081 ttatcgtgag aagaatgtgc cagcagagta acgacttcat ccagcgtgct gcgtagtggg
    31141 aatggaatac tttccagaat cagcttacct gcttccagtt tcgagaagtc gagaacatca
    31201 ttaataattg ccagcaaatt atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc
    31261 gttggtgtta attctgtttt cagcgtcagg cgggtaaagc caataacacc attcagtggt
    31321 gtacgcagct cgtgtgacat atttgccaga aactcggatt taatacgcgc cgcttcctgg
    31381 gcgcgctttt tcgccagatc taactcaacg ttctgaattt ccatctgctc cagcgtttca
    31441 cgcagatcgg acgtcgcctg gtcgatattg tgctgcatct cttcgtgata agcagccagc
    31501 gacattgcca tcgagttgat accgtttttc agcatatcca gctcgccgag cataaatcct
    31561 tccacccggc tgtcgagttg cccgcgacgg atgcggtcga cggtattcac catgttgcga
    31621 atcggaccgg ttacatcgcg cattaagcgc cagccaaaaa taagcgcaat accgatacaa
    31681 aacagcatca tcacgctgga aataaagatc tctttatatt gctgcaagcg aaccgattta
    31741 agatccagct ccagcgcaat atatcccaac atattttgac tatttttggc atcgctactg
    31801 ggcgattcgt cgggggagta actctcagaa ataatcggcg tgcggaggat cataatatcg
    31861 ccgtcacgag tgacagtgag ctggcgagga aacggcacgt tgctgccgag ctgcattgat
    31921 gagggatcaa gatgaaaatt ggaggtgaca aagagtcggt tattttcatc ataaaccgaa
    31981 atcgcgcgaa caatatcgga atggcgacga tgcagtacgc ttattaactg accgatagat
    32041 tcgcgatttt gcaggctcat gccatattca gtagaaactg caagcggctc aataatgctg
    32101 gctccggcat cttccagttg acgctgcaag tcgttatagc gatgcacaac gaaaaagata
    32161 ctcagcaata aaccaataag gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc
    32221 aggctgtagt tggtcatgga gttccgttat gggacaatta aggtcacact gttaaattga
    32281 gaaagtctcg ccaatggcgc aattctactc tgcaaaacga cgcacgacga cgcgtcagat
    32341 cataaccgtt tcagtcaacg acctcgactc ttttggtcag ggcgtggcgc gacataacgg
    32401 caaaacgcta tttatccccg gattattgcc gcaggaaaac gcggaagtta ctgttactga
    32461 agataaaaaa cagtatgccc gcgctaaagt cgtacgccgg ttaagcgata gcccgggacg
    32521 cgaaacgcca cgctgtcctc attttggcgt atgcggcggc tgtcagcaac aacacgccag
    32581 cgtggattta cagcagcgaa gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt
    32641 ctctgaagtg atcgccgatg ttccctgggg ctatcgccgt cgcgcgcgtt taagtctgaa
    32701 ctacttaccg aaaacacagc aacttcagat ggggtttcgc aaagcgggct ccagtgacat
    32761 tgtcgacgtt aaacaatgcc ccattttagt gccccaactt gaagcattgc tgcccaaagt
    32821 catggcatgc ctgggcagct tacaagttat gcgccatctt ggtcatgttg aactggtaca
    32881 ggcaaccagc ggcacgctga tgattttgcg ccataccgca ccgctaagtt cggcagatcg
    32941 cgaaaaactg gaacgctttt cgcattctga aggcctggat ctgtatctcg cccccgatag
    33001 tgagatactc gaaaccgtct ctggtgagat gccctggtat gactcaaatg ggttgcgctt
    33061 aacttttagc ccgcgcgatt ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc
    33121 gcgtgcgttg gaatggctgg atgtgcaacc tgaagatcgc gtactggatt tgttctgcgg
    33181 tatgggcaac tttacactgc cattggcgac acaagctgcc agtgtggtgg gtgtagaagg
    33241 cgttccggcg ctggtggaaa aaggccagca gaatgcgcgt cttaatggct tacagaatgt
    33301 gacgttttat cacgaaaatc ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg
    33361 cttcgataaa gtgttgctgg acccggcgcg agcaggtgcc gcaggtgtta tgcagcaaat
    33421 tataaaactg gaacctattc gtatagttta tgtatcctgt aaccctgcaa cgctggctcg
    33481 ggatagcgaa gcgttattaa aagcaggata taccattgcg cgactggcga tgctggatat
    33541 gttcccacac acgggacatc tggaatcgat ggtacttttc tcgcgcgtta aatagttgcg
    33601 atttgccgat ttcggcaggt ctggtcccta aaggagagga cgatggttgc ggtaagaagt
    33661 gcacatatca ataaggctgg tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt
    33721 accagccaga agtcgtgtga gtgcttagcc gaaacttggg cgtattgtct gcaacagacg
    33781 caggggcatc cggatgccag tctgttattg tggcgtggtg ttgagatggt ggagatcctc
    33841 tcgacattaa gtatggacat tgacacgctg cgggcggcgc tgctgttccc tctggctgat
    33901 gccaacgtag tcagcgaaga tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt
    33961 attcacggcg tgcgtgatat ggcggcgatc cgccagctga aagcgacgca cactgattct
    34021 gtttcctccg aacaggtcga taacgttcgc cggatgttat tggcgatggt cgatgatttt
    34081 cgctgcgtag tcatcaaact ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg
    34141 ccggaagatg aacgtgtact ggcggcaaaa gagtgtacca acatctacgc accgctggct
    34201 aaccgtctcg gaatcggaca actgaaatgg gaactggaag attactgctt ccgttacctc
    34261 catccaaccg aatacaaacg aattgccaaa ctgctgcatg aacggcgtct cgaccgcgaa
    34321 cactacatcg aagagttcgt tggtcatctg cgcgctgaga tgaaagctga aggcgttaaa
    34381 gcggaagtgt atggtcgtcc gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag
    34441 aacctcgcct ttgatgagct gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta
    34501 caggattgct atgccgcact ggggatagtg cacactcact atcgccacct gccggatgag
    34561 tttgacgatt atgtcgctaa cccgaaacca aacggttatc agtctattca taccgtggtt
    34621 ctggggccag gtggcaaaac cgttgagatc caaatccgca ccaaacagat gcatgaagat
    34681 gcagagttgg gtgttgctgc gcactggaaa tataaagagg gcgcggcagc tggcggcgca
    34741 cgttcgggac atgaagaccg gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag
    34801 atggctgatt ccggcgaaat gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg
    34861 tatgtcttta cgccgaaagg tgatgtcgtt gatttgcctg cgggatcaac gccgctggac
    34921 ttcgcttacc acatccacag tgatgtcgga caccgctgca tcggggcaaa aattggcggg
    34981 cgcattgtgc cgttcaccta ccagctgcag atgggcgacc agattgaaat tatcacccag
    35041 aaacagccga accccagccg tgactggtta aacccaaacc tcggttacgt cacaaccagc
    35101 cgtgggcgtt cgaaaattca cgcttggttc cgtaaacagg accgtgacaa aaacattctg
    35161 gctgggcggc aaatccttga cgacgagctg gaacatctgg ggatcagcct gaaagaagca
    35221 gaaaaacatc tgctgccgcg ttacaacttt aatgatgtcg acgagttgct ggcggcgatt
    35281 ggtggcgggg atatccgtct caatcagatg gtgaacttcc tgcaatcgca atttaataag
    35341 ccgagtgccg aagagcagga cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg
    35401 ccgcaaaacc gcagtaaaga taacggtcgc gtggtagtcg aaggtgttgg caacctgatg
    35461 caccacatcg cgcgctgctg ccagccgatt cctggagatg agattgtcgg cttcattacc
    35521 caggggcgcg gtatttcagt acaccgcgcc gattgcgaac aactggcgga actgcgctct
    35581 catgcgccag aacgcattgt tgacgcggta tggggtgaga gctactccgc cggatattcg
    35641 ctggtggtcc gcgtggtggc taatgatcgt agtgggttgt tacgtgatat cacgaccatt
    35701 ctcgccaacg agaaggtgaa cgtgcttggc gttgccagcc gtagcgacac aaaacagcaa
    35761 ctggcaacca tcgacatgac cattgagatt tacaacctgc aggtgctggg ccgcgtgctg
    35821 ggtaaactca accaggtgcc ggatgttatc gacgcgcgtc ggttgcacgg gagttaggca
    35881 gaaatttgct cgtatctaca atgtagattg atatatactg tatctacata tgatagcggt
    35941 ttgaggaaag ggttatgatc cacagtagcg taaagcgttg gggaaattca ccggcggtgc
    36001 ggatcccggc tacgttaatg caggcgctca atctgaatat tgatgatgaa gtgaagattg
    36061 acctggtgga tggcaaatta attattgagc cagtgcgtaa agagcccgta tttacgcttg
    36121 ctgaactggt caacgacatc acgccggaaa acctccacga gaatatcgac tggggagagc
    36181 cgaaagataa ggaagtctgg taatggtaag ccgatacgta cccgatatgg gcgatctgat
    36241 ttgggttgat tttgacccga caaaaggtag cgagcaagct ggacatcgtc cagctgttgt
    36301 cctgagtcct ttcatgtaca acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac
    36361 gcaatcaaaa ggatatccgt tcgaagttgt tttatccggt caggaacgtg atggcgtagc
    36421 gttagctgat caggtaaaaa gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac
    36481 agttgcccca gaggaattac aactcattaa agccaaaatt aacgtactga ttgggtagtg
    36541 ttactaactc acttctattc tggtcacagg tttccacctg acagacccgc aattttcagg
    36601 acaattcaat gaatcaaatc gaccgtttgc tcactattat gcagcgcctg cgcgatccgg
    36661 aaaacggctg cccgtgggat aaagagcaga catttgccac cattgcgcct tacacccttg
    36721 aagaaaccta cgaagtgctg gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg
    36781 aactgggcga tctgctattc caggtggtgt tttacgcgca aatggctcag gaagaagggc
    36841 gctttgactt taatgatatt tgcgctgcta ttagcgataa attagagcgt cgccatccgc
    36901 atgtttttgc tgatagttct gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa
    36961 tcaaaaccga agagcgcgcg cagaaagcgc agcattcggc gctggacgat attcctcgta
    37021 gtttaccggc tttaatgcgg gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg
    37081 actggacgac gcttggcccg gtggttgata aagtctacga agagatcgac gaggtgatgt
    37141 acgaggcgcg gcaggctgtt gtcgaccagg ctaaactgga ggaggaaatg ggggacctgc
    37201 tgtttgccac ggttaatctg gctcgccatt tagggacgaa agcagaaatc gcattgcaaa
    37261 aagcgaacga aaaattcgag cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg
    37321 gactggaaat gacaggtgtt gacctcgaaa caatggaaga agtctggcaa caggtaaaac
    37381 ggcaggaaat tgatctctaa gggaattacg cggtcaagcg ccatttgtgt cattttttaa
    37441 atgacaagcg cttgatttgc gtcaaaaaca tttaccccaa aggggctatt ttctcactcc
    37501 tgatttcaat agtgcgctgg cgaagaggag ggataatgaa agtttgtggc acaggtcatg
    37561 ttcgggtata ctgctttccc gtcctggtta ttccatcgtc ttttcaacct aacttctcag
    37621 gttcagcatg acaacgaact atatttttgt gaccggcggg gtcgtatcct ctctgggtaa
    37681 aggcattgcc gcagcctccc tcgcagccat tcttgaagcc cgtggcctca atgtgaccat
    37741 catgaaactg gatccgtaca tcaacgtcga tccaggtact atgagcccaa tccaacacgg
    37801 ggaagtgttc gttactgaag acggcgctga aaccgacctg gacctggggc actacgagcg
    37861 tttcattcgt accaaaatga gccgccgcaa caacttcacc acgggtcgta tctactctga
    37921 cgttctgcgt aaagaacgcc gcggtgacta cctcggcgca accgtgcagg ttattccgca
    37981 catcactaac gcaatcaaag agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact
    38041 ggtagaaatc ggcggtacag taggtgatat cgaatccttg ccgttcctcg aagcgattcg
    38101 ccagatggct gttgaaattg gccgtgagca cactctgttt atgcacctga cgctggtgcc
    38161 gtacatggca gcgtctggtg aagtcaaaac caaaccgact cagcactctg taaaagagct
    38221 gctctccatc ggtatccagc ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc
    38281 gaacgaacgt gcgaagattg cattgttctg taatgttccg gaaaaagcgg ttatttctct
    38341 gaaagacgtc gattccatct ataaaattcc gggcctgttg aaatctcagg ggctggacga
    38401 ttatatttgt aaacgattca gcttaaactg cccggaagca aatctgtccg aatgggaaca
    38461 ggttatcttc gaagaagcga atccggtaag tgaagtcacc atcggtatgg tcggcaagta
    38521 cattgaactg ccggatgctt ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa
    38581 gaatcgtgtc agcgtcaaca tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt
    38641 tgaaatcctt aaaggtctgg acgcaatcct cgtacctggc ggtttcggct atcgtggcgt
    38701 agaaggcatg attacgaccg cgcgttttgc gcgtgagaac aatattcctt atctgggcat
    38761 ttgcctgggt atgcaggtgg cgttaattga ttacgctcgc catgttgcca acatggagaa
    38821 cgccaactct acggaatttg tgccagactg taagtacccg gttgtggcgc tgattaccga
    38881 gtggcgcgat gaaaacggca acgttgaagt tcgtagcgag aagagcgatc tcggcggtac
    38941 catgcgtctc ggcgcacagc agtgccagtt ggttgacgat agcctggttc gccagctgta
    39001 caatgcgccg acaattgttg agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt
    39061 gaaacagatt gaagatgcag gtctgcgcgt tgcgggccgt tccggggatg atcagttggt
    39121 cgagatcatc gaagttccga atcacccgtg gttcgtggct tgccagttcc atccggagtt
    39181 tacttctact ccacgtgatg gtcacccgct gtttgcaggc tttgtgaaag ctgccagcga
    39241 gttccagaaa cgtcaggcga agtaagtaaa aaagttagaa cggcaacgcg taccttgggt
    39301 acgcgttgtt tgtctggagt ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa
    39361 aatcgtaaaa atcatcggtc gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc
    39421 cgaagtacat ctggagggtg gtttcgtcgg tatggcagct gctccgtcag gtgcttctac
    39481 tggttcccgt gaagctctgg aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg
    39541 cgtaaccaaa gctgttgctg cggtaaacgg cccgatcgct caggcactga ttggcaaaga
    39601 cgctaaagat caggctggca ttgacaagat catgatcgac ctggacggca ccgaaaacaa
    39661 atccaaattc ggcgcgaacg caatcctggc tgtatctctg gctaacgcca aagctgctgc
    39721 tgccgctaaa gggatgccgc tgtacgagca catcgctgaa ctgaacggca ctccgggcaa
    39781 atactccatg ccggttccga tgatgaacat catcaacggt ggtgagcacg ctgacaacaa
    39841 cgttgatatc caggaattca tgattcagcc ggttggcgcg aaaactgtga aagaagccat
    39901 ccgcatgggt tctgaagttt tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa
    39961 cactgcagtt ggtgacgaag gtggctatgc gccgaacctg ggttccaacg ctgaagctct
    40021 ggctgttatc gctgaagctg ttaaagctgc tggttatgaa ctgggcaaag acatcactct
    40081 ggcgatggac tgcgcagctt ctgaattcta caaagacggt aaatacgttc tggctggcga
    40141 aggcaacaaa gctttcacct ctgaagaatt cactcacttc ctggaagaac tgaccaaaca
    40201 gtatccgatc gtatctatcg aagacggtct ggacgaatct gactgggacg gtttcgcata
    40261 ccagaccaaa gttctgggcg acaaaatcca gctggttggt gacgacctgt tcgtaaccaa
    40321 caccaagatc ctgaaagaag gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt
    40381 caaccagatc ggttctctga ccgaaactct ggctgcaatc aagatggcga aagatgctgg
    40441 ctacactgca gttatctctc accgttctgg cgaaactgaa gacgctacca tcgctgacct
    40501 ggctgttggt actgctgcag gccagatcaa aactggttct atgagccgtt ctgaccgtgt
    40561 tgctaaatac aaccagctga ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa
    40621 cggtcgtaaa gagatcaaag gccaggcata agactgactt tatctgattt aaaaatgcca
    40681 gcctcc
//
LOCUS       DAZPLM010000014        39900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPLM010000014 DAZPLM010000000
VERSION     DAZPLM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.014"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(138..813)
                     /locus_tag="ACHVC7_000636"
                     /pseudo
     CDS             complement(138..813)
                     /locus_tag="ACHVC7_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(958..1566)
                     /locus_tag="ACHVC7_000637"
     CDS             complement(958..1566)
                     /locus_tag="ACHVC7_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR2114772.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1750..2067)
                     /gene="metJ"
                     /locus_tag="ACHVC7_000638"
     CDS             complement(1750..2067)
                     /gene="metJ"
                     /locus_tag="ACHVC7_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR2114773.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2344..3504
                     /gene="metB"
                     /locus_tag="ACHVC7_000639"
     CDS             2344..3504
                     /gene="metB"
                     /locus_tag="ACHVC7_000639"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR2114774.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3507..5939
                     /gene="metL"
                     /locus_tag="ACHVC7_000640"
     CDS             3507..5939
                     /gene="metL"
                     /locus_tag="ACHVC7_000640"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR2114775.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6078..6212)
                     /locus_tag="ACHVC7_000641"
     CDS             complement(6078..6212)
                     /locus_tag="ACHVC7_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2114776.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6288..7178
                     /gene="metF"
                     /locus_tag="ACHVC7_000642"
     CDS             6288..7178
                     /gene="metF"
                     /locus_tag="ACHVC7_000642"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR2114777.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7507..9687
                     /gene="katG"
                     /locus_tag="ACHVC7_000643"
     CDS             7507..9687
                     /gene="katG"
                     /locus_tag="ACHVC7_000643"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR2114778.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9781..10686
                     /gene="yijE"
                     /locus_tag="ACHVC7_000644"
     CDS             9781..10686
                     /gene="yijE"
                     /locus_tag="ACHVC7_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR2114779.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10713..11330)
                     /locus_tag="ACHVC7_000645"
     CDS             complement(10713..11330)
                     /locus_tag="ACHVC7_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR2114780.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11604..12707)
                     /gene="gldA"
                     /locus_tag="ACHVC7_000646"
     CDS             complement(11604..12707)
                     /gene="gldA"
                     /locus_tag="ACHVC7_000646"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR2114781.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12718..13380)
                     /gene="fsa"
                     /locus_tag="ACHVC7_000647"
     CDS             complement(12718..13380)
                     /gene="fsa"
                     /locus_tag="ACHVC7_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR2114782.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13392..15893)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_000648"
     CDS             complement(13392..15893)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_000648"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR2114783.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16202..17281
                     /locus_tag="ACHVC7_000649"
     CDS             16202..17281
                     /locus_tag="ACHVC7_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR2114784.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17296..17616
                     /locus_tag="ACHVC7_000650"
                     /pseudo
     CDS             17296..17616
                     /locus_tag="ACHVC7_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17667..19963
                     /locus_tag="ACHVC7_000651"
                     /pseudo
     CDS             17667..19963
                     /locus_tag="ACHVC7_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19929..20807
                     /locus_tag="ACHVC7_000652"
     CDS             19929..20807
                     /locus_tag="ACHVC7_000652"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR2114785.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20809..21150
                     /locus_tag="ACHVC7_000653"
     CDS             20809..21150
                     /locus_tag="ACHVC7_000653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR2114786.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21137..21988)
                     /locus_tag="ACHVC7_000654"
     CDS             complement(21137..21988)
                     /locus_tag="ACHVC7_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2114787.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22203..23936)
                     /locus_tag="ACHVC7_000655"
     CDS             complement(22203..23936)
                     /locus_tag="ACHVC7_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR2114788.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24119..26770)
                     /gene="ppc"
                     /locus_tag="ACHVC7_000656"
     CDS             complement(24119..26770)
                     /gene="ppc"
                     /locus_tag="ACHVC7_000656"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR2114789.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27122..28273)
                     /gene="argE"
                     /locus_tag="ACHVC7_000657"
     CDS             complement(27122..28273)
                     /gene="argE"
                     /locus_tag="ACHVC7_000657"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR2114790.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28427..29431
                     /gene="argC"
                     /locus_tag="ACHVC7_000658"
     CDS             28427..29431
                     /gene="argC"
                     /locus_tag="ACHVC7_000658"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR2114791.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29439..30215
                     /gene="argB"
                     /locus_tag="ACHVC7_000659"
     CDS             29439..30215
                     /gene="argB"
                     /locus_tag="ACHVC7_000659"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR2114792.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30276..31649
                     /gene="argH"
                     /locus_tag="ACHVC7_000660"
     CDS             30276..31649
                     /gene="argH"
                     /locus_tag="ACHVC7_000660"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR2114793.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31916..32833
                     /gene="oxyR"
                     /locus_tag="ACHVC7_000661"
     CDS             31916..32833
                     /gene="oxyR"
                     /locus_tag="ACHVC7_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR2114794.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32816..34216)
                     /gene="sthA"
                     /locus_tag="ACHVC7_000662"
     CDS             complement(32816..34216)
                     /gene="sthA"
                     /locus_tag="ACHVC7_000662"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR2114795.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34537..35187
                     /gene="fabR"
                     /locus_tag="ACHVC7_000663"
     CDS             34537..35187
                     /gene="fabR"
                     /locus_tag="ACHVC7_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR2114796.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35187..35546
                     /locus_tag="ACHVC7_000664"
     CDS             35187..35546
                     /locus_tag="ACHVC7_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR2114797.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35586..36686)
                     /gene="trmA"
                     /locus_tag="ACHVC7_000665"
     CDS             complement(35586..36686)
                     /gene="trmA"
                     /locus_tag="ACHVC7_000665"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR2114798.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36800..36990
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37055..38899
                     /gene="btuB"
                     /locus_tag="ACHVC7_000666"
     CDS             37055..38899
                     /gene="btuB"
                     /locus_tag="ACHVC7_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR2114799.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38844..39701
                     /gene="murI"
                     /locus_tag="ACHVC7_000667"
     CDS             38844..39701
                     /gene="murI"
                     /locus_tag="ACHVC7_000667"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR2114800.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9108 a        10032 c        11005 g         9755 t
ORIGIN      
        1 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
       61 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
      121 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
      181 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
      241 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
      301 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
      361 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
      421 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
      481 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
      541 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
      601 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
      661 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
      721 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg
      781 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
      841 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt
      901 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
      961 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     1021 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     1081 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     1141 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     1201 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     1261 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     1321 ttcaccatgg gcgatccact gcttgagcgg ggtgtatttc accgggccga ccgcttcaaa
     1381 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     1441 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     1501 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     1561 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     1621 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     1681 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     1741 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     1801 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     1861 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     1921 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     1981 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     2041 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     2101 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     2161 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     2221 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     2281 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     2341 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     2401 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     2461 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     2521 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     2581 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     2641 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     2701 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     2761 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     2821 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     2881 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     2941 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     3001 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     3061 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     3121 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     3181 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     3241 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     3301 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     3361 atgacacatg caggcatggc accagaagcg cgtgctgccg tcgggatctc cgagacgctg
     3421 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     3481 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     3541 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     3601 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     3661 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     3721 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     3781 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     3841 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     3901 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     3961 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     4021 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     4081 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     4141 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     4201 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     4261 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     4321 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     4381 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     4441 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     4501 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     4561 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     4621 cgctgaaaat cctcgacgaa gcgggattac ctggcgaact gcgcctgcgt caggggctgg
     4681 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     4741 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     4801 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     4861 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     4921 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     4981 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     5041 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     5101 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     5161 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     5221 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     5281 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     5341 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     5401 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     5461 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     5521 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     5581 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     5641 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     5701 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     5761 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     5821 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     5881 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     5941 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     6001 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     6061 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     6121 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     6181 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     6241 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     6301 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     6361 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     6421 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     6481 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     6541 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     6601 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     6661 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     6721 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     6781 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     6841 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     6901 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     6961 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     7021 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     7081 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     7141 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     7201 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     7261 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     7321 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     7381 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     7441 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     7501 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     7561 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     7621 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     7681 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     7741 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     7801 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     7861 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     7921 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     7981 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     8041 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     8101 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     8161 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     8221 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     8281 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     8341 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     8401 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     8461 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     8521 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     8581 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     8641 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     8701 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     8761 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     8821 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     8881 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     8941 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
     9001 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
     9061 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
     9121 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
     9181 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
     9241 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
     9301 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
     9361 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
     9421 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
     9481 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
     9541 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
     9601 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
     9661 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
     9721 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
     9781 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
     9841 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
     9901 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
     9961 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    10021 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    10081 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    10141 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    10201 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    10261 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    10321 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    10381 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    10441 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    10501 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    10561 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    10621 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    10681 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    10741 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    10801 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    10861 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    10921 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    10981 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    11041 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    11101 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    11161 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    11221 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    11281 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    11341 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    11401 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    11461 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaac aagaaaattc aatatattgc
    11521 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    11581 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    11641 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    11701 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    11761 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    11821 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    11881 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    11941 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    12001 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    12061 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    12121 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    12181 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    12241 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    12301 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    12361 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    12421 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    12481 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    12541 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    12601 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    12661 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    12721 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    12781 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    12841 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    12901 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    12961 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    13021 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    13081 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    13141 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    13201 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    13261 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    13321 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    13381 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    13441 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    13501 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    13561 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    13621 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    13681 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    13741 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    13801 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    13861 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    13921 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    13981 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    14041 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    14101 tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    14161 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    14221 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    14281 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    14341 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    14401 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    14461 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    14521 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    14581 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    14641 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    14701 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    14761 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    14821 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    14881 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    14941 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    15001 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    15061 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    15121 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    15181 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    15241 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    15301 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    15361 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    15421 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    15481 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    15541 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    15601 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    15661 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    15721 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    15781 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    15841 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    15901 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    15961 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    16021 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    16081 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    16141 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    16201 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    16261 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    16321 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    16381 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    16441 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    16501 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    16561 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    16621 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    16681 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    16741 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    16801 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    16861 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    16921 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    16981 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    17041 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    17101 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    17161 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    17221 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    17281 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc
    17341 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    17401 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    17461 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    17521 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    17581 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    17641 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    17701 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    17761 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    17821 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    17881 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    17941 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    18001 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    18061 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    18121 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    18181 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    18241 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    18301 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    18361 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    18421 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    18481 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    18541 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    18601 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    18661 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    18721 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    18781 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    18841 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    18901 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    18961 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    19021 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    19081 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    19141 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    19201 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    19261 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    19321 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    19381 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    19441 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    19501 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    19561 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    19621 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    19681 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    19741 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    19801 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    19861 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    19921 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    19981 gcgatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    20041 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    20101 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    20161 aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata
    20221 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    20281 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    20341 ttttacagcg actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc
    20401 cagcaagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa
    20461 ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga
    20521 atctgcgttt gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg
    20581 gtttcacgct cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa
    20641 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    20701 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    20761 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    20821 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg
    20881 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga
    20941 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    21001 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    21061 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg
    21121 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    21181 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    21241 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    21301 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    21361 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    21421 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    21481 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    21541 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    21601 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    21661 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    21721 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    21781 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    21841 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    21901 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    21961 agagcagata gctgacgtcg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    22021 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    22081 aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc
    22141 gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat
    22201 aattactgat tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt
    22261 ttcttgtacg ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa
    22321 cgggttggat cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt
    22381 tccgccaggc tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc
    22441 catttttccg acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct
    22501 tcgttacgcc cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag
    22561 aagtaaacca ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc
    22621 acatgatcgt tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg
    22681 cttaatcctg gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag
    22741 cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca
    22801 gggtcattca gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc
    22861 tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc
    22921 atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc
    22981 tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg
    23041 gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta
    23101 ttaaacacgg tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca
    23161 cgcggataac cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact
    23221 aaagtgcgcg gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta
    23281 ttttcattca gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc
    23341 aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca
    23401 aacggcttgt ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc
    23461 agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc
    23521 cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa
    23581 tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg
    23641 ctctgcgaga actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc
    23701 catagcacca cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc
    23761 gggatcaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa
    23821 ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc
    23881 caacccaggg ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta
    23941 agaacctgtc ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa
    24001 ggtatattca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga
    24061 acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt
    24121 agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga
    24181 cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg
    24241 cctgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc
    24301 acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt
    24361 cttcttgcag gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt
    24421 gatcatagta ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga
    24481 gacgcgtcga gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat
    24541 cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt
    24601 tttgcgtcca ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc
    24661 gacgtttcgc cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg
    24721 agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg
    24781 agatgactga cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca
    24841 gcaggttggc ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg
    24901 gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca
    24961 ggcttcctgg cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg
    25021 aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta
    25081 atgcatcctg tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg
    25141 agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga
    25201 tattgagcag ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca
    25261 gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca
    25321 gtacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg
    25381 cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt
    25441 tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg
    25501 cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt
    25561 cggtatggcg ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt
    25621 tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct
    25681 gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc
    25741 cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca
    25801 tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc
    25861 caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct
    25921 gaatatcttt caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg
    25981 tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac
    26041 ggaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca
    26101 gttcgcgcaa gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc
    26161 atttggcttc atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact
    26221 gggcgattaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt
    26281 tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac
    26341 gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt
    26401 ttttgatggt gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga
    26461 tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg
    26521 tgttggctag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg
    26581 acaaattttg taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag
    26641 atttcgacaa cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct
    26701 tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt
    26761 gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg
    26821 tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa
    26881 aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta
    26941 cgccattaaa ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc
    27001 atacataaca ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt
    27061 gcggcaccgg agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt
    27121 tttaatgcca gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa
    27181 accgcgtctc cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca
    27241 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    27301 tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    27361 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca
    27421 ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ccaggcagcg
    27481 gacgaatatc catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt
    27541 gaatatgccc gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt
    27601 ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag
    27661 cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt
    27721 tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg
    27781 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    27841 cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc
    27901 gtagcgtatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    27961 gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg
    28021 gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca
    28081 gcatattgaa tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat
    28141 ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    28201 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    28261 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    28321 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    28381 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    28441 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    28501 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    28561 ctccgatttg catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga
    28621 tatcagcgag tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag
    28681 ccacgattta gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc
    28741 gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc
    28801 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    28861 agcgaatttg attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc
    28921 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    28981 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    29041 accgtatggc gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga
    29101 cgttatcttc accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    29161 ccgcctgaaa tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc
    29221 ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    29281 gctgccattt tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac
    29341 cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt
    29401 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    29461 aactgggcgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg
    29521 tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    29581 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga
    29641 cgcctgctga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    29701 tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    29761 acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag
    29821 gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca
    29881 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc
    29941 tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg
    30001 gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    30061 gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc
    30121 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta
    30181 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    30241 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    30301 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    30361 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg
    30421 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gaacgtgctg ctggaagatg
    30481 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    30541 aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc
    30601 gtaacgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac
    30661 tgacggctaa ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg
    30721 cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt
    30781 gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc
    30841 gtctggatgt cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc
    30901 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg
    30961 tttctgaccg tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc
    31021 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    31081 tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    31141 tggagctgat tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga
    31201 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    31261 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    31321 ttcaggtgaa acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg
    31381 aactggcgga ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg
    31441 gtgaagcggt ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg
    31501 agttgcagaa attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat
    31561 cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga
    31621 ttgcttttgc gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc
    31681 ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac
    31741 gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa
    31801 aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc
    31861 gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa
    31921 tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc
    31981 agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga
    32041 gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat
    32101 gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc
    32161 aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg
    32221 accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat
    32281 gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga
    32341 ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga
    32401 tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc
    32461 gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga
    32521 tcaggcaatg ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac
    32581 cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc
    32641 gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc
    32701 ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta
    32761 tgagcagctg gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa
    32821 acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt
    32881 taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg
    32941 cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct
    33001 cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg
    33061 cgcgtgccag atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca
    33121 gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga
    33181 tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc
    33241 cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa
    33301 cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt
    33361 ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga
    33421 gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac
    33481 agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt
    33541 gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt
    33601 tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca
    33661 ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc
    33721 tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc
    33781 aggcaataac aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg
    33841 ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt
    33901 cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga
    33961 tatcggcaaa agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga
    34021 attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg
    34081 tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa
    34141 ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat
    34201 cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta
    34261 acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg
    34321 cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat
    34381 actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt
    34441 ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat
    34501 agtgccagca gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa
    34561 aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc
    34621 agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct
    34681 ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt
    34741 ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg
    34801 atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg
    34861 ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa
    34921 attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt
    34981 gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag
    35041 gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg
    35101 cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt
    35161 attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc
    35221 tgctggcgtt agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca
    35281 ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc
    35341 aacgttatct taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta
    35401 ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta
    35461 acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta
    35521 accgtaagca ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat
    35581 gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga
    35641 tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat
    35701 aacgtttccg ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg
    35761 gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc
    35821 tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat
    35881 tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat
    35941 tgcgcagcag caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc
    36001 gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag
    36061 cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg
    36121 gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg
    36181 tcgatgtaat cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca
    36241 ttcagattct gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca
    36301 tccagcttct tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa
    36361 tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc
    36421 gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt
    36481 ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac
    36541 tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa
    36601 aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt
    36661 tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt
    36721 gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg
    36781 gacagtaact tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat
    36841 ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc
    36901 ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc
    36961 cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc
    37021 tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac
    37081 ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt
    37141 cgttactgct aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt
    37201 gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct
    37261 tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg
    37321 cggtacaaat gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg
    37381 ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata
    37441 tatccgtggg ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat
    37501 catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta
    37561 tcagaactat gatgtctcta cgcagcaaca actgggggat aagacacggg taacgctgtt
    37621 gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca
    37681 agcgcagaca gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa
    37741 ctttactgat gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta
    37801 tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag
    37861 ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta
    37921 tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac
    37981 gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg
    38041 tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt
    38101 tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg
    38161 cgattttacc tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg
    38221 aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta
    38281 cgggacatct tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa
    38341 tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt
    38401 gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca
    38461 caccctgaaa tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa
    38521 ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc
    38581 gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg
    38641 gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa
    38701 ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct
    38761 tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt
    38821 cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc
    38881 tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg
    38941 ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt
    39001 tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg
    39061 tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca
    39121 acactgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg
    39181 gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac
    39241 tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta
    39301 atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc
    39361 tacatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa
    39421 tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag
    39481 aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc
    39541 gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata
    39601 ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt
    39661 acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga atgttgcgcg
    39721 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    39781 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaatct
    39841 caacagagaa aagcaaaaaa aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
//
LOCUS       DAZPLM010000015        36671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPLM010000015 DAZPLM010000000
VERSION     DAZPLM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.015"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHVC7_000668"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHVC7_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR2114801.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHVC7_000669"
     CDS             complement(280..873)
                     /locus_tag="ACHVC7_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR2114802.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHVC7_000670"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHVC7_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2114803.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHVC7_000671"
     CDS             1788..2183
                     /locus_tag="ACHVC7_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR2114804.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHVC7_000672"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHVC7_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR2114805.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHVC7_000673"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHVC7_000673"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR2114806.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHVC7_000674"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHVC7_000674"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR2114807.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHVC7_000675"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHVC7_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR2114808.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHVC7_000676"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHVC7_000676"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR2114809.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHVC7_000677"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHVC7_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR2114810.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHVC7_000678"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHVC7_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR2114811.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHVC7_000679"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHVC7_000679"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR2114812.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHVC7_000680"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHVC7_000680"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR2114813.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHVC7_000681"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHVC7_000681"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR2114814.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGCEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHVC7_000682"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHVC7_000682"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR2114815.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHVC7_000683"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHVC7_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR2114816.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHVC7_000684"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHVC7_000684"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR2114817.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHVC7_000685"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHVC7_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR2114818.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHVC7_000686"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHVC7_000686"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR2114819.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHVC7_000687"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHVC7_000687"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR2114820.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHVC7_000688"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHVC7_000688"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR2114821.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHVC7_000689"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHVC7_000689"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR2114822.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHVC7_000690"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHVC7_000690"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR2114823.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVDYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHVC7_000691"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHVC7_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR2114824.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHVC7_000692"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHVC7_000692"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR2114825.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHVC7_000693"
     CDS             complement(26207..26548)
                     /locus_tag="ACHVC7_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR2114826.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHVC7_000694"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHVC7_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR2114827.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHVC7_000695"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHVC7_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR2114828.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHVC7_000696"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHVC7_000696"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR2114829.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHVC7_000697"
     CDS             33227..34570
                     /locus_tag="ACHVC7_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR2114830.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHVC7_000698"
     CDS             complement(34667..34873)
                     /locus_tag="ACHVC7_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR2114831.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHVC7_000699"
     CDS             35198..35752
                     /locus_tag="ACHVC7_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR2114832.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..>36671)
                     /gene="ytfI"
                     /locus_tag="ACHVC7_000700"
     CDS             complement(35842..>36671)
                     /gene="ytfI"
                     /locus_tag="ACHVC7_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR2114833.1"
                     /translation="NFFNNIQHAFKKKDWISNYDSLLTLREFFRCATQIDKNSYQVLS
                     SKNGTVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEEIPITINEYNSFMDFKNREFP
                     LEQNRRLYACDILQKKQSDIPKRIKGYIHQAIGDVSLGYALLEDIVSKLKRGKFELQI
                     PGGGIKECDGWYIHEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLI
                     YNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8876 a         9832 c         9123 g         8840 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtattgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggctgcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agtccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg t
//
LOCUS       DAZPLM010000016        34981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPLM010000016 DAZPLM010000000
VERSION     DAZPLM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.016"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHVC7_000701"
     CDS             complement(<1..815)
                     /locus_tag="ACHVC7_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2114834.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHVC7_000702"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHVC7_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHVC7_000703"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHVC7_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHVC7_000704"
     CDS             complement(1397..1873)
                     /locus_tag="ACHVC7_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR2114835.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHVC7_000705"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHVC7_000705"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR2114836.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHVC7_000706"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHVC7_000706"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR2114837.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHVC7_000707"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHVC7_000707"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR2114838.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHVC7_000708"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHVC7_000708"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR2114839.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGALPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHVC7_000709"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHVC7_000709"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR2114840.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHVC7_000710"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHVC7_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR2114841.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHVC7_000711"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHVC7_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR2114842.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHVC7_000712"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHVC7_000712"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR2114843.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHVC7_000713"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHVC7_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR2114844.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHVC7_000714"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHVC7_000714"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR2114845.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHVC7_000715"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHVC7_000715"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR2114846.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHVC7_000716"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHVC7_000716"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR2114847.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHVC7_000717"
     CDS             13839..14543
                     /locus_tag="ACHVC7_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR2114848.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14814..15461
                     /locus_tag="ACHVC7_000718"
     CDS             14814..15461
                     /locus_tag="ACHVC7_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR2114849.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(15526..16237)
                     /locus_tag="ACHVC7_000719"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="ACHVC7_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHVC7_000720"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHVC7_000720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR2114850.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTRWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="ACHVC7_000721"
     CDS             complement(17475..18236)
                     /locus_tag="ACHVC7_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR2114851.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="ACHVC7_000722"
     CDS             18369..18779
                     /locus_tag="ACHVC7_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR2114852.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="ACHVC7_000723"
     CDS             complement(18741..19847)
                     /locus_tag="ACHVC7_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2114853.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="ACHVC7_000724"
     CDS             complement(19858..21000)
                     /locus_tag="ACHVC7_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2114854.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHVC7_000725"
     CDS             complement(20993..22729)
                     /locus_tag="ACHVC7_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR2114855.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHVC7_000726"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHVC7_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR2114856.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHVC7_000727"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHVC7_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR2114857.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHVC7_000728"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHVC7_000728"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR2114858.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHVC7_000729"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHVC7_000729"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR2114859.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHVC7_000730"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHVC7_000730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR2114860.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHVC7_000731"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHVC7_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR2114861.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHVC7_000732"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHVC7_000732"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR2114862.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHVC7_000733"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHVC7_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR2114863.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHVC7_000734"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHVC7_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR2114864.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHVC7_000735"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHVC7_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR2114865.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHVC7_000736"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHVC7_000736"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR2114866.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHVC7_000737"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHVC7_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8269 a         8976 c         9491 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggagcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacct ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg cattttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZPLM010000017        34897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPLM010000017 DAZPLM010000000
VERSION     DAZPLM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.017"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..3261
                     /gene="evgS"
                     /locus_tag="ACHVC7_000738"
     CDS             <1..3261
                     /gene="evgS"
                     /locus_tag="ACHVC7_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR2114867.1"
                     /translation="ALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDS
                     MRPLTSSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSN
                     IITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEV
                     SQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMG
                     DILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFIT
                     TPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVK
                     EGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKAL
                     NAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSV
                     RRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAML
                     PLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASD
                     HAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLE
                     LLSGSGLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQ
                     NTCHSFGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKI
                     TTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKE
                     LIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDH
                     PTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLR
                     EQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQY
                     RHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHG
                     AANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(3317..4462)
                     /gene="yfdE"
                     /locus_tag="ACHVC7_000739"
     CDS             complement(3317..4462)
                     /gene="yfdE"
                     /locus_tag="ACHVC7_000739"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR2114868.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4536..5480)
                     /gene="yfdV"
                     /locus_tag="ACHVC7_000740"
     CDS             complement(4536..5480)
                     /gene="yfdV"
                     /locus_tag="ACHVC7_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR2114869.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5550..7244)
                     /gene="oxc"
                     /locus_tag="ACHVC7_000741"
     CDS             complement(5550..7244)
                     /gene="oxc"
                     /locus_tag="ACHVC7_000741"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR2114870.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(7298..8548)
                     /gene="frc"
                     /locus_tag="ACHVC7_000742"
     CDS             complement(7298..8548)
                     /gene="frc"
                     /locus_tag="ACHVC7_000742"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR2114871.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(9061..9684)
                     /locus_tag="ACHVC7_000743"
     CDS             complement(9061..9684)
                     /locus_tag="ACHVC7_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR2114872.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            9981..10256
                     /gene="ypdI"
                     /locus_tag="ACHVC7_000744"
     CDS             9981..10256
                     /gene="ypdI"
                     /locus_tag="ACHVC7_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR2114873.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(10333..10575)
                     /locus_tag="ACHVC7_000745"
     CDS             complement(10333..10575)
                     /locus_tag="ACHVC7_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR2114874.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            10928..11848
                     /gene="lpxP"
                     /locus_tag="ACHVC7_000746"
     CDS             10928..11848
                     /gene="lpxP"
                     /locus_tag="ACHVC7_000746"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR2114875.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            12204..12275
                     /gene="ypdK"
                     /locus_tag="ACHVC7_000747"
     CDS             12204..12275
                     /gene="ypdK"
                     /locus_tag="ACHVC7_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR2114876.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(12340..13578)
                     /gene="alaC"
                     /locus_tag="ACHVC7_000748"
     CDS             complement(12340..13578)
                     /gene="alaC"
                     /locus_tag="ACHVC7_000748"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR2114877.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            13955..15652
                     /gene="ypdA"
                     /locus_tag="ACHVC7_000749"
     CDS             13955..15652
                     /gene="ypdA"
                     /locus_tag="ACHVC7_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR2114878.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            15667..16401
                     /gene="ypdB"
                     /locus_tag="ACHVC7_000750"
     CDS             15667..16401
                     /gene="ypdB"
                     /locus_tag="ACHVC7_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR2114879.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            16414..17271
                     /locus_tag="ACHVC7_000751"
     CDS             16414..17271
                     /locus_tag="ACHVC7_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2114880.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(17274..19769)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_000752"
                     /pseudo
     CDS             complement(17274..19769)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(19794..20831)
                     /gene="ypdE"
                     /locus_tag="ACHVC7_000753"
     CDS             complement(19794..20831)
                     /gene="ypdE"
                     /locus_tag="ACHVC7_000753"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR2114881.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(20831..21916)
                     /gene="ypdF"
                     /locus_tag="ACHVC7_000754"
                     /pseudo
     CDS             complement(20831..21916)
                     /gene="ypdF"
                     /locus_tag="ACHVC7_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(21931..23178)
                     /locus_tag="ACHVC7_000755"
     CDS             complement(21931..23178)
                     /locus_tag="ACHVC7_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR2114882.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(23200..23526)
                     /locus_tag="ACHVC7_000756"
     CDS             complement(23200..23526)
                     /locus_tag="ACHVC7_000756"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR2114883.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(23746..24711)
                     /gene="glk"
                     /locus_tag="ACHVC7_000757"
     CDS             complement(23746..24711)
                     /gene="glk"
                     /locus_tag="ACHVC7_000757"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR2114884.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            24915..26171
                     /locus_tag="ACHVC7_000758"
     CDS             24915..26171
                     /locus_tag="ACHVC7_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR2114885.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            26286..26612
                     /gene="ypeC"
                     /locus_tag="ACHVC7_000759"
     CDS             26286..26612
                     /gene="ypeC"
                     /locus_tag="ACHVC7_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR2114886.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(26753..27991)
                     /locus_tag="ACHVC7_000760"
     CDS             complement(26753..27991)
                     /locus_tag="ACHVC7_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR2114887.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            28327..29529
                     /gene="nupC"
                     /locus_tag="ACHVC7_000761"
     CDS             28327..29529
                     /gene="nupC"
                     /locus_tag="ACHVC7_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR2114888.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(29579..31767)
                     /locus_tag="ACHVC7_000762"
                     /pseudo
     CDS             complement(29579..31767)
                     /locus_tag="ACHVC7_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(31976..32051)
                     /locus_tag="ACHVC7_000763"
     tRNA            complement(31976..32051)
                     /locus_tag="ACHVC7_000763"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32016..32018),aa:Ala,seq:ggc)
     gene            complement(32091..32166)
                     /locus_tag="ACHVC7_000764"
     tRNA            complement(32091..32166)
                     /locus_tag="ACHVC7_000764"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32131..32133),aa:Ala,seq:ggc)
     gene            32387..32746
                     /locus_tag="ACHVC7_000765"
     CDS             32387..32746
                     /locus_tag="ACHVC7_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR2114889.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            32748..33140
                     /locus_tag="ACHVC7_000766"
     CDS             32748..33140
                     /locus_tag="ACHVC7_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR2114890.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(33192..34607)
                     /gene="gltX"
                     /locus_tag="ACHVC7_000767"
     CDS             complement(33192..34607)
                     /gene="gltX"
                     /locus_tag="ACHVC7_000767"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR2114891.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         9109 a         8878 c         8162 g         8748 t
ORIGIN      
        1 gcgctggaag aaggtgaagt cgatatagtg ttatcacatt tagtagcttc gccgcctctt
       61 aatgatgaca ttgctgcaac caaaccactg ataattacct ttccggcgct ggtaaccacc
      121 cttcacgatt caatgcgacc gcttacctca tcaaaaccag taaatattgc tcgagtagca
      181 aattatcccc ctgacgaggt aattcatcaa tcatttccaa aagcaacaat tatctctttt
      241 acaaatttat atcaggcatt agcatccgtc tcagccggac agaatgatta ctttattggt
      301 agtaacatca ttaccagcag tatgatttcc cgctatttca ctcactcctt aaatgtagtg
      361 aaatattata actcaccgcg tcaatataat tttttcttga ccagaaaaga atctgtcgtt
      421 cttaatgaag tactcaatcg atttgttgat gctttgacaa atgaagttcg ctatgaagta
      481 tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaaaccatt agaactcact
      541 gaacatgaaa aacaatggat taagcagcat cccgatttaa aggtgctgga aaatccttac
      601 tcccctccct attctatgac ggatgaaaat ggctcggttc ggggcgttat gggggatatt
      661 cttaatatta ttactttgca aacaggttta aatttttctc cgatcaccgt ttcacacaat
      721 atccatgctg gaacacagct gaatcccggc ggatgggata tattacccgg tgctatttat
      781 agtgaagata gagaaaataa tgttttattt gctgaagcct tcataacaac gccttacgtt
      841 tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc
      901 attccatatt attatgagct tcattcgcaa ttaaaagaga tgtatccgga ggtagagtgg
      961 ataaaagtcg ataacgccag cgctgcattt cacaaggtca aggaaggcga acttgatgct
     1021 ctggtcgcga cacagttaaa ttcacgttac atgatcgacc attactatcc taatgaactt
     1081 tatcattttc ttattcccgg cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga
     1141 gaaccggaac ttaaggatat tattaataaa gcactgaatg caatcccccc aagcgaagtt
     1201 ctgcgcctga ccgaaaaatg gattaaaatg cccaatgtga ccattgacac atgggacctc
     1261 tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg cagtagcctt
     1321 ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagtcattca gggtgattta
     1381 gaaaaccaaa tatcattccg aaaagcactc tcggattcct taccgaatcc aacttatgtt
     1441 gtaaactggc aaggtaatgt cattagtcat aatagtgctt ttgaacatta tttcactgcg
     1501 gattactaca aaaatgcaat gttaccatta gaaaacagtg actccccctt taaagatgtt
     1561 ttttctaatg cgcatgaagt cacagcagaa acgaaagaaa atcgaacaat atacacacag
     1621 gtttttgaaa ttgataatgg catcgagaaa agatgcatta atcactggca tacattatgc
     1681 aatctgccag ccagcgatca tgctgtttat atttgtggtt ggcaagatat tacagagacg
     1741 cgtgatttaa ttcatgcact cgaagtagag agaaataaag cgatcaatgc aactgtcgca
     1801 aaaagtcagt ttctggcaac aatgagtcac gaaataagaa caccaataag ctccattatg
     1861 ggcttcctgg aacttctgtc gggttctggt cttagcaagg agcaacgggt ggaggcgatt
     1921 tcacttgcct actccaccgg acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc
     1981 gacaaaattg aatcgggtaa ctatcaactt caaccacaat gggtcgatat ccctacttta
     2041 gtccagaaca cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat cgcattaatt
     2101 tgcagcagta catttcctga tcattacctg gttaagatcg accctcaggc gtttaagcag
     2161 gtcttatcaa atttactgag caatgctctc aaatttacca ccgagggggc agtaaaaatt
     2221 acgacctccc tggttcacat tgatgacaac cacgctgtta tcaaaatgac gattatggat
     2281 tctggaagtg gattatcgca ggaagaacaa caacaactct ttaaacgcta cagccaaaca
     2341 agtgcaggtc gccagcaaac aggatctggc ttaggcttaa tgatctgcaa agaattaatt
     2401 aaaaatatgc agggcgattt gtcattagaa agtcatccag gcataggaac aacatttacg
     2461 atcacaatcc cggtagaaat tagccagcaa gtggcgactg tcgaggcaaa agcagaacac
     2521 cccatcacac tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc gaccaacagg
     2581 ctattactca aacgccagct aaatctatta ggatatgatg ttgatgaagc cactgatggt
     2641 gtgcaagcgc tacacaaagt cagtatgcaa cattatgatc tgcttattac tgacgttaat
     2701 atgccgaata tggatggttt tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta
     2761 cccatctggg ggcttacagc caacgcacag gctaacgaac gtgaaaaagg gttaagttgc
     2821 ggcatgaact tatgtttgtt caaaccgttg accctggatg tactgaaaac acatttaagt
     2881 cagttacacc aggttgcgca tattgcacct cagtatcgcc accttgatat cgaagccctg
     2941 aaaaataata cggcgaacga tctacaactg atgcaggaga ttctcatgac tttccagcat
     3001 gaaacgcata aagatctacc cgctgcgttt caagcactag aagctggcga taacagaact
     3061 ttccatcagt gtattcatcg catccacggt gcggctaaca tcctgaattt gcaaaagttg
     3121 attaatatta gccatcagtt agaaataaca cctgtttcag atgacagtaa gcctgaaatt
     3181 cttcagttgc tgaactctgt aaaagaacac attgcagagc tggaccagga gattgctgtt
     3241 ttctgtcaga aaaataacta aatagcggct cccacaatgt tctaatgtgg gagctattta
     3301 ccagcacatc ttacatttat gatgagaact cctggcgaat ttgttcccca tgctggtcga
     3361 gcgttgccgc tcccggcata acatgcgggt ccgcgcagcc gctgattttt atcggattac
     3421 ccggcatcat tattcctccg gcttcaatca acatatttct cgcctgagtt tgtggcaaat
     3481 taatggcctc agccacactt aataacggcg cgacgggcac accaacttca tgtattttgg
     3541 ctaaccaaac ttcagctgcc tgcgttttta acgttcgctc aatatattgt ttaagaatag
     3601 cctggttttg tacgcgtaaa atattgctgc taaatcgggg atcattaacc agttccgtaa
     3661 gctccagtgc ctggcataac gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg
     3721 gcttatcctg agtatcgaaa acatcaaaag gtgccatgta gggatggcga tttcccaggc
     3781 gttgtggtga cttccctgtc gcaatatatg ccatcagacc atgctccaga aaactcagcg
     3841 tggcatcaaa catcgctata tcgacatgcg cccctctctg gctcttttcg cggccataaa
     3901 gtgcactcac tattccgctg aataaataga caccgccgca tagatccgca agagaggtac
     3961 caacacgcac tggcggagca tcagggtacc ctgtttccat cattatccca ctcattgcct
     4021 gaatgatggt atcgtaggca ggagcatctt ttagcggacc ggtatgtccg aaacctgacg
     4081 atgaagcata tatgaggcgc gggttgattt cttgtaacct ttcccatgaa aaccccagtt
     4141 tttccattgt acctgggcga aaattctcag ctaatacatc agcttgtttg agcatattta
     4201 taaatatact tttatcgtga tcattcttta aatcaagaac cacactctct ttgccatgat
     4261 taataaaact gtaatagagt gactgtccat ccacataggg accaaatgtg cgggtatcat
     4321 caccatgacc tggtggctca actttaatta cccttgcgcc catattacaa agaagttgag
     4381 ttccgaaagg tccattaagg acatgtgtca tatcgataac taataagcct tcaaacggcc
     4441 ctttgctttc attatttgtc atttgcttat gccttgaaaa aagccgaagg tgaaaagaca
     4501 cttcggcatt tatgtaaaat caataacatg attaattatg aaaccagtcg actgacataa
     4561 atccacaagg gagccgtgac gacaaagccc agtacgctca cagccaatga cgctgtacca
     4621 gtacgggtgt agacattaaa ccggctggca ataatgattc cggagaatgc cggcggtaat
     4681 gcgcctgcca gtaccatcat ctgcaaatgt tcgctgttca aatgacatgc cataccaacg
     4741 agaagcagcg ccagtggcat cagaatcaac ttcaggaagg tgttataagc aatttcggca
     4801 ctgaactcga atttatgtgc agccagagtc aaccccgcag cgaataccgc tacccctgag
     4861 ttagccttcg caatcagatt aaaggtcggg tcccatgctg ccggaatttt taccccaacc
     4921 agcaccagga tcgttgccag aacaggtgcc catactactg gctcctttgc cgcagaaatt
     4981 aatgcgctca gattactatt cttcttacca tccgctcctg aggaaggatt cagcaaatac
     5041 agaccaatag gaatagtaat tgcgttaaca ataatagaaa taattgctac cactaaacct
     5101 gttgatacgg aatcaccata aataggatcg agaactgcaa accccaggaa tccaatggta
     5161 ggtgaacccg caattaatgc acatacagcg gcttctgcat gggtacgttt aaaaaatttg
     5221 tagcaaccaa accaggagaa aaagaaacat ccgacaataa ccacaagtga tacaagggtc
     5281 agacgagtgt ccgcaaaaat catttccctg tttgcccggg taatagaaac aaatagagcc
     5341 gcaggaagag cgtagtttaa taccagttta ttaaatgcgc gggcttgatc ttctgaaaaa
     5401 gtttctcgtc tgccgctaaa ataacccaac agcatgatta cgataatagg caataaatcg
     5461 ccaataaaaa atgttagcat aatagtattt ccgataaggg tcccctcacc atcgcataat
     5521 gagttggcaa gaggagacga tgtcagtatt taattaccag cgacttgttt tgggttaagt
     5581 ttggtaatat ggccactttc agttcctgca gcagggtcga tgaccacatt aataatggtc
     5641 ggtttgcgcg actgaatacc ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg
     5701 ttatagccaa cgccacgaaa cgcatccatt aatttgtcat accttgcatg gtgcaacaga
     5761 tccgttggtg atggtgcacc agcgccactg agatcaacac cgtctcctct gtagatgccg
     5821 ccattattaa aaataacgat cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc
     5881 atcccactga aaccaaaagc actatcacct tcaatggcga caaccggaga accagaagtc
     5941 acgctagcac cgatggcata gcccataccg atgcccatga caccccaggt accacaatcc
     6001 agacgacgac gtggtttata catatcaata atatttcgtg cattatccag ggtatttgca
     6061 ccttcattaa ctaaataaat atcctggttc tcgcgcaata cgtcgcgcac agcacttaat
     6121 gcattaaagt aatttaatgg ttgagtatct gtacttaatt tttcatgcat tttttgtgca
     6181 ttttgctgct tgtggatatt taaaatatcg cgccatacca gtggagtcgt aaatgtgttt
     6241 tgtttcagtt ctgccagcat accttgcata ctggatgcaa tatcaccaac gactggcaca
     6301 gcaatggggc ggttgctgtc aatttcctgc ggttcaatat ccagttgaat aaactgtgta
     6361 tctgccgccc atcctttttt accgtgtgcc aataaccaat tcagtcgtgc accaacaagc
     6421 atgacaacgt cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc
     6481 gtatcttcaa ggatcccttt cgccatagac attggcagga atggaatctg agcactttca
     6541 ataaattcac gaagctgttc atcagcttgt gaatacgccg cgcctttgcc aaggataatt
     6601 aatggccgtt cagcttttgc taaaagcgaa attgcgctag tgactgactt cgggcatggc
     6661 aataatgctg gcgacggatt ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc
     6721 atcgtcgcgg ccaggacatt tgctggcaaa tcaagataaa ctccgccagg gcgacccgat
     6781 acagagaccc ggatagcgcg tgccaatgca atgccaagat cctgcggctg attaacgcga
     6841 aatgctgctt tggcatacgg ttttgccgca ttcatttggt ccagctcttc ataatcacct
     6901 tgctgtaggt cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat cggaaaacca
     6961 tttaccgttg cgttggccaa tgcggtcaaa ccattgagga atcctggcgc agaaactgtc
     7021 aggcagatcc ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac
     7081 tgctcatgac gaaaaccaat ataacgaatg ccttccgcct gggcatggcg tgccatatcc
     7141 gtcacaggaa tacctacaac accataaata gtgtcaatat tattctgttt taatgcttca
     7201 acgatgatat gcataccatc tgtcatttga agttgatctg acatgattaa taacctcgaa
     7261 atttttataa attgggagcc agaagccccc gttaatatca gatggcgtgg ttttgcttca
     7321 ttgcagcaat ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg gtatgttcac
     7381 ctaatagcgg cgcagcttta atatccggcg taaaggcaga gaatttcatt ggacaaccaa
     7441 cggtcagata ttttccacgc aacggttgtt ccacttcaac aacactgcca ctttggcgca
     7501 aagagggatc aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga atatcaaact
     7561 gagtcaaata ggccaccgct tcatgtttat caatagtgac agtgtatttt tcgatttcag
     7621 caaaaatatc gaaaatatgt ggctgtcggg catgggctgt actgtatgcc gaatcggtaa
     7681 tccattctgg tttgccgatg gctttacagg tgttttccca gttttgctcc tgaatagtga
     7741 aataaatata ggcgttagga tcggtttccc agcctttaca tttcaggatc cagccaggct
     7801 gaccgccacc acctgcatta ccaccgcggg gaactgcatc accaaatgta ccattcggat
     7861 actgcgggta ttcttccaga taacccaatt tatcgagacg ctgctggtca cgtaatttca
     7921 cgcggcaaag gttcaatacg gcatcctgca ttgacatggt gactcgttgc ccacgccccg
     7981 ttttttcgcg atgcagcaaa gcagcaagta aaccgatcag caaatgcatt ccggtgttgc
     8041 tgtcacccaa cgctgcagcg cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg
     8101 ccgcgccacc cgctgcctga gcaacgtttt cataggcttt tacattcaca taaggcgaac
     8161 actcatcaaa ccctttgatc gaaccaaaaa tcagacgtgg attgatttct tgaatatgct
     8221 cccaggtgaa gcccatgtga tcaatggccc ctggatgaaa gttctcgact aagatatcag
     8281 cttcgcggat cagcttttcc attacctctt tgccttccgc tgttttggta tttaactcaa
     8341 tagaacgttt gttactgtta agcatggtga agtaaagcgc atcgatatca ggaatatctc
     8401 gcagttggtg acgcgttacg tcaccaacgc cgggacgttc aattttaata acgtcagcgc
     8461 caaaccaggc cagcatttga gtacaagatg ggccagattg cacaccggtg aaatcgagaa
     8521 ctttaattcc ttgaagtgga gttgacatag caatacctat tagttaatta aatatgtgtt
     8581 aatttagcgt ttatttaagg cataacgccc tctataaata atctgcaaaa tatcttcgta
     8641 gcggcactcg atatttttaa attcctgtat caagtactga aaattatttc atcgccactt
     8701 ccaattcctt tctgcatggt aagtagtgag gacgaaaaga agaatatgat aaatatttat
     8761 tcgtggtaat cagagagagt cgcgacattt gtgatgttat tcttacaact gtaagaagcg
     8821 ccggtgattt acaagtaaga gtatgattac gctttttaaa gcgtaccata aagcataaca
     8881 taataaatga tttgtgatga cactggatta ttgcggttta ctttgaggaa aataaaatgc
     8941 gtgtttatag aaccgcgagc gccaaataat tttatgacca caacctgtcg tattgtggtc
     9001 ataaaaaatt gtcggctgga gggagttaaa tcctttacct caatatactg cctgtcagca
     9061 ttagttcaca aacagtgctt cgctatcgac gatgattcca tcttccgcac ctttaagcgc
     9121 caggtttgcc tcgtagtatt gctttttatc aagcagtttc tgcgcatcag caagtgcatt
     9181 acgagtctgt ttaagcggca tcagatattg gttttccatt acgccaacac cggccaggcg
     9241 aagctcttcc attgcgccct ttttatcccc tttcgccatt ttttcattgg caatttttat
     9301 cgcggcttct ttttcgggtg ttgctacata actctcagaa attccgacag atgcattaat
     9361 gacaatatat tggtcatcat tgagattggt ctttttacct ggcttagcga actttgccca
     9421 ttcggtactg tcatctgaca gcaaagcgga agcttcattg gtcagttctt ttgccttttc
     9481 cggatcgcca tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg catataaacc
     9541 ttgttcagag atacgctctg ctgctgccgt tttctgggtt tgctgagttt gctgttgtgc
     9601 cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat
     9661 cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg
     9721 ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt
     9781 tatcttacaa ttgtagttat tatcgcaaag cggcatttag tcttacagtt gtaggaatat
     9841 gcttcctgtc gcagagaact attcttatat accacaaaga gatgtcattt attatattga
     9901 tgtaaatatt gattaattat atgtaaatac agccataaca tcgtatggtt ctcagtgaac
     9961 cattttcagg ataactttct atgaaagtaa acttaatact tttcagctta tttttattgg
    10021 tctctattat ggcatgcaat gtttttgcat tttccatttc tggtggtgta agtgagagga
    10081 gctataaaga gactgaaaaa acatcagcga tgacaaccac acactctaca aaacttcagc
    10141 catcacaggc gattttgttt aagatgagag aagatgcgcc accattaaac ctcacagaag
    10201 aaatcacgcc tacttatccg acaaaggcga attatcttat tcatcctgtg cgatagcgtc
    10261 tgtcacatgg caggtgaaca atcatgtctg atgctgatgt gaacacctgc ccaaatgagc
    10321 catcctgacc cactatcgcc tgccataccc caccataaag cgaatcatca agccacaagc
    10381 cggggcaaga aacataaata tccccgtcac cagttcatta ccgccagtta accacatggt
    10441 gaagtaataa atgagtgcag cgaggatcac catgccgaag aaagaagaga ccgccgatac
    10501 cacgttcagt acctgaccga tgtagccgct tacgcagaca atacacaggg cgagaaacat
    10561 ccataaataa atcatagctc aacctcctgc cgcgctaaaa aacctgtaac ttcagtcact
    10621 ttaatagtaa gacaatcctt aacctctggc aatttttgct aagcatcaat gaaaacagca
    10681 cgttaaatgc aacgctattg cgtgcggaaa attttttact ttgcacgatt aataatcagt
    10741 agctgaaagc agtcagccag tgataaagta gagccaccgc attcatgctc ctcgtggtta
    10801 tgtcctgact agtctttaca ctctttacag gaaccattgt cgtacatgat ggcccaacca
    10861 attgagtgta ccgctgtcga catggaatct tctgcgatac aacaattcgt atctacagaa
    10921 ggtaactatg tttccacaat gcaaattttc ccgcgagttt ctacatcctc gctactggct
    10981 cacatggttt gggcttggtg tactctggct ttgggtacag cttccttatc ctgttctctg
    11041 ctttctcggc acgcgtattg gcgcaatggc gcgaccattc ctgaaacgtc gtgaatctat
    11101 cgcccgtaaa aacctggaac tttgtttccc gcagcattct gcggaagaac gcgagaagat
    11161 gattgccgaa aactttcgtt cactcggcat ggcgctggta gaaaccggca tggcatggtt
    11221 ctggcccgac agtcgcgtac gtaaatggtt tgatgttgaa gggttggata accctaaacg
    11281 cgcacaaatg caaaatcgcg gcgtaatggt tgtcggcgtc cattttatgt cgctggaact
    11341 gggcggccgc gtgatgggac tgtgccaacc aatgatggct acctatcgtc cacataataa
    11401 tcagctgatg gaatgggtgc agacccgtgg gcgcatgcgc tctaacaaag cgatgatcgg
    11461 cagaaataat ctgcgcggca ttgtcggtgc actgaagaaa ggtgaagcgg tatggtttgc
    11521 tcccgatcag gattatggtc gtaaaggcag ctccttcgcg ccgttctttg cggtggaaaa
    11581 tgtcgccaca accaatggca cctatgttct ctcccgtctc tctggcgcag ccatgttgac
    11641 cgtaacgatg gtaagaaaag cggattacag cggatatcgt ttgttcatca ccccagagat
    11701 ggaaggctac ccgacagatg aaaatcaagc cgctgcctat atgaacaaga ttatcgagaa
    11761 agagatcatg cgcgcaccgg agcagtacct ctggatccac cgtcgcttta aaacgcgccc
    11821 ggtgggagaa tcgtcgttgt acatttaaca aactgctctt actctttccc tcctcaaatc
    11881 ggccataata acctcatggt ttttatggct tttttcttaa ggttttttcc ttcgctgaat
    11941 atctaattat taccgtcaga aattaaactg tcgccgatat gagacacttg taagttacta
    12001 aaagtaaata agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat taacgccgtc
    12061 ttgagcagtt tagctcagaa ttctggggtg atgcccacgg taaaatacag ggcttttaag
    12121 gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc aggcgtaaca
    12181 gtaattacgc ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca
    12241 tcgtttgggc cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag
    12301 gagccatcag gctcctgttt gcatttctga cgacggtgtt tattccgcgt tttcgtgaat
    12361 atgtttgctg ctggcgggta gtaaaccgtc ggcacggaac atcgctttaa tccctctgat
    12421 ggcctgacga atacggtcgc ggttttcaat cagtgcaaag cgaacatgcg tatcaccata
    12481 gtcgccaaag ccaatccccg gcgagacaca aaccttcgct tcgttaagga gcttcttggc
    12541 aaattccagc gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga catacatcga
    12601 agccttcggc atttcgacca tccagcccgc ttcatgcagc cctttaacca atacatcacg
    12661 gcggcgtttg tactgttcgg taatgtcgcg cacgcactgt tgatcgccct cgagtgccgc
    12721 aatcgccgcc acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt tgattcgcgc
    12781 cagggcactg accagcgttt tgttgccgac cataaagccg atacgccagc ccgccatgtt
    12841 gtagcttttc gacagcgtaa agaactcgac cgccacatcg cgtgcacccg gcacctgcat
    12901 aattgacggc gctttccagc catcgtagac gatatcggca taggccaggt catggaccac
    12961 cagcacatcg tagcgtttcg ccagcgccac aaccttttcg aagaactcca gttccacgca
    13021 ttgcgcggtt gggttcgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc
    13081 acgaatggcg cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg
    13141 tacctgcgcc ccggcaatca ccgcgccata aatatggatc gggtaacttg gattcggcac
    13201 cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga
    13261 accaatagtg acgatggctt ctgattccgg gtcgatttca acgtcgtagc gatcctgata
    13321 ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt
    13381 gtccgggcgc tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc
    13441 cgggttaccc atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag
    13501 ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc
    13561 agggcgagtg tcagccataa tttcctcaga ttaacgttag cgcccggacc gtccgagcga
    13621 cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct gtccagcgtt
    13681 ttagaacatt tttatcaatg gctttaaatg aggaactcca cttcaaaaac aggaaatttt
    13741 tctacgctaa caagaaaatt accaccaatt ttattaacat tataatttca ataaatttac
    13801 cagccagaag tgcattatca agagatacat tgctaataca ccatttatct ttcaatcccc
    13861 ttttaaattt atggtttttc ctcaaaaacg gcctgctggt catcagcctt caggttacct
    13921 atcatagagg tttaatcctt attcagagtc acccgtgcac gaaatattca acatgctgct
    13981 ggcggtcttc gatcgggcag cgttaatgct tatctgcctg ttctttctca tccgtatccg
    14041 cctgtttcgc gaactgttgc acaagtcggc gcactcccca aaagaattgc tcgccgttac
    14101 cgccattttc tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg tagaaggctc
    14161 gctggtgaac gtacgtatta tcgcggtgat gtccgacggg attctgtttg gcccgtgggt
    14221 aggcatcatt accggcgtga ttgcgggtat tcaccggtat ttaattgata tcggcggcgt
    14281 gacggcgatc ccctgcttta tcaccagcat tctggcgggt tgtatatcag gctggataaa
    14341 cctgaaaatc cccaaagcac agcgctggcg cgtcggtatt ctcggcggca tgttgtgtga
    14401 gacgttgacc atgattctgg taattgtctg ggcaccaact accgcgttgg ggatcgatat
    14461 cgtctctaaa atcggcattc caatgatcct cggtagcgtc tgtatcggct ttattgtgct
    14521 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcagg ccaagctggc
    14581 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
    14641 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
    14701 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
    14761 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
    14821 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
    14881 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
    14941 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
    15001 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
    15061 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
    15121 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
    15181 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
    15241 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
    15301 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
    15361 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
    15421 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
    15481 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
    15541 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
    15601 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
    15661 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
    15721 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
    15781 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
    15841 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
    15901 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
    15961 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
    16021 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
    16081 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
    16141 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
    16201 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
    16261 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
    16321 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
    16381 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
    16441 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
    16501 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
    16561 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
    16621 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
    16681 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
    16741 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
    16801 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
    16861 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
    16921 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
    16981 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
    17041 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
    17101 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
    17161 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
    17221 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
    17281 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
    17341 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
    17401 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
    17461 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
    17521 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
    17581 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
    17641 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
    17701 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
    17761 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
    17821 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
    17881 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
    17941 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
    18001 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
    18061 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcggagaag
    18121 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
    18181 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
    18241 cataagatct gatcgaggct gtgaaccatc gggatcatca actgggcgtt gccgaaactg
    18301 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
    18361 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
    18421 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
    18481 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
    18541 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
    18601 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
    18661 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
    18721 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
    18781 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
    18841 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
    18901 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
    18961 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
    19021 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
    19081 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
    19141 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
    19201 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
    19261 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
    19321 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
    19381 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
    19441 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
    19501 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
    19561 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
    19621 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
    19681 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
    19741 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
    19801 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
    19861 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
    19921 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
    19981 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
    20041 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
    20101 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
    20161 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
    20221 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
    20281 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
    20341 gctttcccca tcacccgctg gtgagggaga acctgaaaag ttgtatcaaa ggtgacgcga
    20401 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
    20461 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
    20521 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
    20581 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
    20641 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
    20701 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
    20761 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
    20821 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
    20881 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
    20941 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
    21001 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
    21061 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
    21121 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
    21181 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
    21241 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
    21301 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
    21361 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    21421 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    21481 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    21541 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    21601 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    21661 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    21721 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    21781 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    21841 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
    21901 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
    21961 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
    22021 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaaa tagcggattc
    22081 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
    22141 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    22201 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    22261 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
    22321 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaaactgaa
    22381 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
    22441 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
    22501 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
    22561 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    22621 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
    22681 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    22741 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
    22801 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
    22861 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
    22921 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    22981 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    23041 catgcgtgaa atccctaaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    23101 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    23161 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    23221 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    23281 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
    23341 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
    23401 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
    23461 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
    23521 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    23581 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    23641 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
    23701 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
    23761 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
    23821 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
    23881 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
    23941 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
    24001 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
    24061 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
    24121 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
    24181 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
    24241 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
    24301 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
    24361 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
    24421 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
    24481 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    24541 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    24601 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    24661 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    24721 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    24781 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    24841 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    24901 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    24961 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    25021 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    25081 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    25141 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    25201 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    25261 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    25321 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    25381 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    25441 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    25501 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    25561 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    25621 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    25681 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    25741 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    25801 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    25861 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    25921 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    25981 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    26041 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    26101 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    26161 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    26221 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    26281 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    26341 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    26401 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    26461 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    26521 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    26581 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    26641 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    26701 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    26761 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    26821 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    26881 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    26941 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    27001 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    27061 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    27121 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    27181 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    27241 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    27301 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    27361 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    27421 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    27481 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    27541 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    27601 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    27661 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    27721 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    27781 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    27841 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    27901 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    27961 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    28021 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    28081 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    28141 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    28201 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    28261 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    28321 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    28381 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    28441 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    28501 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    28561 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    28621 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    28681 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    28741 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    28801 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    28861 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    28921 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    28981 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    29041 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    29101 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    29161 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    29221 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    29281 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    29341 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    29401 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    29461 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    29521 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    29581 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    29641 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    29701 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    29761 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    29821 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    29881 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    29941 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    30001 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    30061 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    30121 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    30181 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    30241 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    30301 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    30361 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    30421 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    30481 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    30541 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    30601 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    30661 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    30721 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    30781 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    30841 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    30901 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    30961 aacagcccag gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    31021 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    31081 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    31141 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    31201 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    31261 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    31321 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    31381 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    31441 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    31501 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    31561 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    31621 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    31681 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    31741 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    31801 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    31861 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    31921 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    31981 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    32041 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    32101 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    32161 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    32221 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    32281 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    32341 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    32401 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    32461 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    32521 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    32581 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    32641 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    32701 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    32761 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    32821 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    32881 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    32941 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    33001 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    33061 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    33121 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    33181 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    33241 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    33301 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    33361 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    33421 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    33481 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    33541 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    33601 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    33661 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    33721 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    33781 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    33841 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    33901 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    33961 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    34021 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    34081 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    34141 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    34201 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    34261 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    34321 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    34381 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    34441 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    34501 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    34561 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    34621 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    34681 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    34741 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    34801 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    34861 agcgggggtg attagctcag ctgggagagc acctccc
//
LOCUS       DAZPLM010000018        33565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPLM010000018 DAZPLM010000000
VERSION     DAZPLM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.018"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..235
                     /locus_tag="ACHVC7_000768"
     CDS             <1..235
                     /locus_tag="ACHVC7_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR2114892.1"
                     /translation="KFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLLR
                     GWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            238..1095
                     /locus_tag="ACHVC7_000769"
     CDS             238..1095
                     /locus_tag="ACHVC7_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR2114893.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1116..3107
                     /locus_tag="ACHVC7_000770"
     CDS             1116..3107
                     /locus_tag="ACHVC7_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR2114894.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3108..3629)
                     /gene="sodC"
                     /locus_tag="ACHVC7_000771"
     CDS             complement(3108..3629)
                     /gene="sodC"
                     /locus_tag="ACHVC7_000771"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR2114895.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3710..4606)
                     /locus_tag="ACHVC7_000772"
     CDS             complement(3710..4606)
                     /locus_tag="ACHVC7_000772"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR2114896.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4655..4894)
                     /locus_tag="ACHVC7_000773"
     CDS             complement(4655..4894)
                     /locus_tag="ACHVC7_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR2114897.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4997..5596
                     /gene="nemR"
                     /locus_tag="ACHVC7_000774"
     CDS             4997..5596
                     /gene="nemR"
                     /locus_tag="ACHVC7_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR2114898.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            5633..6730
                     /gene="nemA"
                     /locus_tag="ACHVC7_000775"
     CDS             5633..6730
                     /gene="nemA"
                     /locus_tag="ACHVC7_000775"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR2114899.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6811..7218
                     /gene="gloA"
                     /locus_tag="ACHVC7_000776"
     CDS             6811..7218
                     /gene="gloA"
                     /locus_tag="ACHVC7_000776"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR2114900.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7321..7968
                     /gene="rnt"
                     /locus_tag="ACHVC7_000777"
     CDS             7321..7968
                     /gene="rnt"
                     /locus_tag="ACHVC7_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR2114901.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8061..12630
                     /locus_tag="ACHVC7_000778"
                     /pseudo
     CDS             8061..12630
                     /locus_tag="ACHVC7_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(12681..13028)
                     /gene="grxD"
                     /locus_tag="ACHVC7_000779"
     CDS             complement(12681..13028)
                     /gene="grxD"
                     /locus_tag="ACHVC7_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR2114902.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13362..14177
                     /gene="mepH"
                     /locus_tag="ACHVC7_000780"
     CDS             13362..14177
                     /gene="mepH"
                     /locus_tag="ACHVC7_000780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR2114903.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14305..14886
                     /gene="sodB"
                     /locus_tag="ACHVC7_000781"
     CDS             14305..14886
                     /gene="sodB"
                     /locus_tag="ACHVC7_000781"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR2114904.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15032..16207)
                     /locus_tag="ACHVC7_000782"
     CDS             complement(15032..16207)
                     /locus_tag="ACHVC7_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2114905.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16373..16462)
                     /gene="cydH"
                     /locus_tag="ACHVC7_000783"
                     /gene_synonym="ynhF"
     CDS             complement(16373..16462)
                     /gene="cydH"
                     /locus_tag="ACHVC7_000783"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR2114906.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16761..17786
                     /gene="purR"
                     /locus_tag="ACHVC7_000784"
     CDS             16761..17786
                     /gene="purR"
                     /locus_tag="ACHVC7_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR2114907.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17783..18715)
                     /gene="punR"
                     /locus_tag="ACHVC7_000785"
     CDS             complement(17783..18715)
                     /gene="punR"
                     /locus_tag="ACHVC7_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR2114908.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18828..20039
                     /gene="punC"
                     /locus_tag="ACHVC7_000786"
     CDS             18828..20039
                     /gene="punC"
                     /locus_tag="ACHVC7_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR2114909.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYCGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            20330..21478
                     /gene="cfa"
                     /locus_tag="ACHVC7_000787"
     CDS             20330..21478
                     /gene="cfa"
                     /locus_tag="ACHVC7_000787"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR2114910.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21518..22159)
                     /gene="ribE"
                     /locus_tag="ACHVC7_000788"
     CDS             complement(21518..22159)
                     /gene="ribE"
                     /locus_tag="ACHVC7_000788"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR2114911.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22374..23747
                     /gene="mdtK"
                     /locus_tag="ACHVC7_000789"
     CDS             22374..23747
                     /gene="mdtK"
                     /locus_tag="ACHVC7_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR2114912.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(23788..25044)
                     /locus_tag="ACHVC7_000790"
     CDS             complement(23788..25044)
                     /locus_tag="ACHVC7_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR2114913.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25352..25428
                     /locus_tag="ACHVC7_000791"
     tRNA            25352..25428
                     /locus_tag="ACHVC7_000791"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25386..25388,aa:Val,seq:gac)
     gene            25433..25509
                     /locus_tag="ACHVC7_000792"
     tRNA            25433..25509
                     /locus_tag="ACHVC7_000792"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25467..25469,aa:Val,seq:gac)
     gene            25617..25922
                     /locus_tag="ACHVC7_000793"
     CDS             25617..25922
                     /locus_tag="ACHVC7_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR2114914.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26048..27652
                     /locus_tag="ACHVC7_000794"
     CDS             26048..27652
                     /locus_tag="ACHVC7_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR2114915.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27664..28476)
                     /gene="ydhT"
                     /locus_tag="ACHVC7_000795"
     CDS             complement(27664..28476)
                     /gene="ydhT"
                     /locus_tag="ACHVC7_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR2114916.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28480..29265)
                     /gene="phsC"
                     /locus_tag="ACHVC7_000796"
     CDS             complement(28480..29265)
                     /gene="phsC"
                     /locus_tag="ACHVC7_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR2114917.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29262..29930)
                     /locus_tag="ACHVC7_000797"
     CDS             complement(29262..29930)
                     /locus_tag="ACHVC7_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2114918.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(29994..30632)
                     /locus_tag="ACHVC7_000798"
     CDS             complement(29994..30632)
                     /locus_tag="ACHVC7_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR2114919.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30645..32747)
                     /locus_tag="ACHVC7_000799"
     CDS             complement(30645..32747)
                     /locus_tag="ACHVC7_000799"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR2114920.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32768..33394)
                     /locus_tag="ACHVC7_000800"
     CDS             complement(32768..33394)
                     /locus_tag="ACHVC7_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR2114921.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8034 a         8864 c         8277 g         8390 t
ORIGIN      
        1 gaagtttatg ctcaatgcaa caggattgcc cttacaagat ctggtgttcg gtgcgtccgt
       61 ctattttcct ccgtttttca aagcattcgc gtttggattc gtcatctggc ttgtcgtaca
      121 ccgcctgctt cgtggctgga tctacgccgg tgacatctgg catcccttgt taatggattt
      181 atcgctgttt gcgatttgcg tttgccttgc tctggcaata ctgattgcgt ggtaactatg
      241 tcaattaaaa caattaagta tttctcaaca atcattgtag cggtagttgc ggttcttgcc
      301 ggatggtggc tgtggaatta ttacatgcaa tctccgtgga cacgcgacgg gaaaatacgc
      361 gcagaacagg tttctatcac cccacaggtg tcgggacgta ttgttgagct gaatataaaa
      421 gacaaccagc tggttaacgc aggggatctt ctgctcacga tcgataaaac gccctttcag
      481 atcgccgagc tgaacgcaca ggctcagctg gcgaaagcgc aatctgacct cgcaaaagcc
      541 aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt ttatttctgc cgaagaactc
      601 gataccgcta acctcaatgt taaagcgatg caggccagtg ttaatgccgc acaggccacg
      661 ctgaaacaga cacaatggca actggcgcaa acggaaattc gcgccccggt gagtggacgg
      721 gtgactaacc tcactacccg catcggcgat tacgctgaca ccgggaaacc gctttttgcc
      781 cttgtcgaca gccactcgtt ttacgtcatt ggttattttg aagaaaccaa gttgcgccat
      841 atccgcgaag gtgcaccagc acaaattacc ctctatagtg ataacaaaac gttacagggt
      901 cacgtttcca gtatcggtcg ggcgatttat gatcagagcg ttgaaagtga ctcaagcctt
      961 attccggatg tgaaacctaa cgtcccctgg gttcgtctcg cccaacgcgt tcccgtccgc
     1021 tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt cgggcaccac ctgtagcatt
     1081 gccgtaggtc aataatgaac gcatcgtaat ggtccttgcg caatttgccc tggttcaggg
     1141 ccacgctggc gcaatggcgt tatgcgttac gcaataccat tgccatgtgt ctggcgctga
     1201 cggttgccta ttatttgaat ctggatgaac cctattgggc gatgacctcg gctgcagtgg
     1261 ttagctttcc caccgttggc ggtgtcatca gcaaaagcct cggacgcatc gctggcagtt
     1321 tactgggagc cattgcggca ctgcttcttg ccggacatac gctcaatgag ccgtggtttt
     1381 ttctattgag catgtcggcg tggcttggct tttgtacctg ggcctgtgcg cacttcacga
     1441 ataacgtcgc gtatgcattt caactggcgg gctacacggc tgccatcatc gcctttccga
     1501 tggttaatat tactgaggcc agccagctgt gggatatcgc tcaggcgcgc gtttgcgagg
     1561 tgatagtcgg tattttgtgc ggcggcatga tgatgatgat cctgcctagc agttccgatg
     1621 ctactgccct tttaactgca ttgaaaaaca tgcacgcccg attactggaa catgccagtt
     1681 tactctggca gcctgaaaca aacgatgcca ttcgtgcagc acatgagggg gtgattgggc
     1741 agatactgac catgaatttg ctgcgtatcc aggctttctg gagccactat cgttttcgcc
     1801 agcaaaacgc gcgccttaat gcgctactcc accagcaatt acgtatgacc agtgtcatct
     1861 ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc aggtgccacg cgagaaattc
     1921 tcgaacaatt gctgactgcg ctcgccagtt cgcaaacaga tgcttacacc gtcgcacgta
     1981 ttatcgcccc gctacgcccg accaacgtcg ccgactaccg gcacgtcgcc ttctggcagc
     2041 gactacgtta tttttgccgc ctttatctgc aaagtagtca ggaattacat cgtctgcaaa
     2101 gcggtgtaga tgatcatgcc agactcgcac ggacatccgc tctggctcgt cataccgaca
     2161 acgccgaagc tatgtggagc gggctgcgta cattttgtac gttgatgatg attggcgcat
     2221 ggagtattgc atcgcaatgg gatgccggtg ccaatgcatt aacgctggca gcaattagct
     2281 gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt gtcactgctg atgcgcacgc
     2341 tggtgttact ttcgctattc agctttgtgg tcaaatttgg cctgatggtc cagattagcg
     2401 atctgtggca atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat
     2461 tgcagatgcc caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta
     2521 tcgctgtcac taatcccccg gtgtatgatt ttgctgattt tcttaacgag aatctggcaa
     2581 aaatcgttgg cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg
     2641 ctcgtaaaag ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc
     2701 gccatccaac actgagtgga agcgaatttg aatcgctcac ttatcatcac gtcagtcagt
     2761 tgagtaacag ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc
     2821 tgaactgttc tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat
     2881 cgcgagtacg ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc
     2941 agcaaaaatc actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc
     3001 gtcatcatca acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc
     3061 tttcgcaact tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca
     3121 ccacaggcat agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg
     3181 ccgccaacgt ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc
     3241 gcgatgacgg catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct
     3301 aaatgcccgg taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg
     3361 cctgcggatt ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg
     3421 gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc
     3481 agacctttat cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac
     3541 gtgacgaggt tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc
     3601 gccagaatgg ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata
     3661 agtgtaaacc agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt
     3721 cgtaccccag tgccgcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt
     3781 ctgcttcgac agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct
     3841 gcgacggtaa acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact
     3901 cctccgccac cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac
     3961 gaccaccacc aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga
     4021 gcgtgccatc cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa
     4081 gggtaaacgg cagacgtgat ttcaacaggg caaattgcgc aggcgtaaag ttcgatacgc
     4141 caaaatgacg cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg
     4201 catccattaa cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat
     4261 taattagcga ctgttcggcg ctcttaatga tgtgatcgcg gtcagtgatg taatgaccaa
     4321 tgacgttttc ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt
     4381 cacgcaggtg aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac
     4441 cataaatatc agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac
     4501 tgaccagctg gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac
     4561 gggaaaactc cgggccttgc ggcgcaatag taatacgctg aaccataatc gcttcctctt
     4621 atcagatatg agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt
     4681 cgtctgatga cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc
     4741 gcagcacctc ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac
     4801 tacgaaaaca gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc
     4861 tctggacggg aaagaactct aattgctccg ccacttcgcc ctcctcaaat aagattatta
     4921 ccattattga agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc
     4981 tactacactc caacgcatga acaaacacac cgaacatgat actcgcgaac atctcctggc
     5041 gacgggcgag caactttgcc tgcaacgtgg gttcaccggg atggggctaa gcgaattact
     5101 aaaaatcgct gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt
     5161 tggcgttgct atgcttgagc gccattacgc cacatatcac cagcgactga ctgagttgct
     5221 gcaatccggc gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa
     5281 ccagttttgc caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt
     5341 gtgcgatctg tcagaagata tgcgtagcgc gatggataaa ggcgctcgcg gcgtgattgc
     5401 cctgctctct caggcgctgg aaaatggcca tgagaaccac tgtttaacct tttgtggcga
     5461 accgctgcaa caggcacaag tgctttacgc actatggctg ggtgcgaatc tgcaggccaa
     5521 aatttcgcgc aattccgagc cactggaaaa cgcgctggca catgtaaaaa ccattattgc
     5581 gacgcctgcc gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc
     5641 tgaaaaactg tattccccac tgaaagtggg cgcaataact gcggcaaacc gtatttttat
     5701 ggcaccgctg acgcgtctgc gcagtattga accgggtgac atccctaccc cgttgatgga
     5761 ggaatactat cgccaacgtg ccagtgccgg tttgattatt agcgaagcca cgcaaatttc
     5821 tgcccaggca aaagggtatg ctggtgcacc tggcatccat agtccggaac aaattgccgc
     5881 atggaaaaaa attaccgctg gcgttcatgc tgaaaatggt catatggccg tacagctgtg
     5941 gcacactgga cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc
     6001 ttcagcactt agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg
     6061 tgttgaaaca tccatgccgc gtgcgcttga actggaagag attccaggta tcgtcaatga
     6121 tttccgtcag gccattgcta acgcgcgtga agccggtttt gatctggtag agctccactc
     6181 tgctcacggt tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca
     6241 gtacggcggc agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat
     6301 tgaagaatgg ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa
     6361 cacggataac ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa
     6421 acgcggcatt gcttatctgc atatgtcaga accagattgg gcgggaggtg aaccgtatac
     6481 tgatgcgttc cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc
     6541 atacacggta gaaaaagccg aaacgctgat tggcaaaggg ttaattgatg cggtggcatt
     6601 tggtcgtgac tggattgcga acccggatct ggtcgcccgc ttgcagcgca gagctgagct
     6661 taacccacag cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc
     6721 gacgttgtaa tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt
     6781 gaatctgtta gccattttta ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt
     6841 ggcgatctgc aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt
     6901 accagcgaaa acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc
     6961 gaagaagcgg tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact
     7021 gcttatggtc acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt
     7081 caaaacgggg gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc
     7141 gcgtttgtgg aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt
     7201 cgcggtctgg gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg
     7261 catcgacaag taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag
     7321 atgtccgata acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt
     7381 gtgatcgatg ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc
     7441 gccatcaccc tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc
     7501 cacgtcgaac catttgtcgg cgcaaatttg cagccagagg ccctcgcctt caacggcatt
     7561 gacccgaacg atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt
     7621 aaagttgtac gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac
     7681 aatgccaatt ttgatcacag ctttatgatg gccgccgcag aacgcgcctc actgaaacgt
     7741 aacccgttcc accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga
     7801 caaaccgtat tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg
     7861 cactccgcgc tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc
     7921 tggaaacgtc tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc
     7981 ctgatgacat gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat
     8041 attcacgggg aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg
     8101 tgttttcact ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc
     8161 agtcgcaaac ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg
     8221 gctccgggaa aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg
     8281 gcgaagatac ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga
     8341 taaaagccct gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg
     8401 atgaacggcg gcggcgcggc gaaacggaag tcaatcttcg cgtagggatc cgtactggcg
     8461 atacgcctgc acaggaacgc agcaaactca cccgtaatcc gccggatatt ctgatcacca
     8521 cacccgaatc actctatctg atgctgacct cccgcgcgcg cgaaacgcta cgcggcgtcg
     8581 aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt gcgcatctgg
     8641 cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga attggccttt
     8701 ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcaat cgcccggtta
     8761 cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta ccggtcgcca
     8821 atatggatga tgtctcatcg gtcgccagca gcaccggcga agacagccat gccggccggg
     8881 aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg cgccatcgct
     8941 cgaccattgt ctttactaat tcacgtgggc tggcggaaaa actgacggca cgattaaatg
     9001 agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg gcccatttcg
     9061 agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt attgcccgct
     9121 cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgagcaggcg ctgaaatcgg
     9181 gtgagttacg ctgcgtggtc gcaacctcca gtctcgaact ggggattgat atgggcgcag
     9241 tggatctgat gattcaggtg gcaacgccgc tttctgttgc cagtgggtta caacgcattg
     9301 gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttccct cgtacccggc
     9361 gtgacttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg ctggaaaacc
     9421 tgacaccgcc gcataatcca ctcgacgtcc ttgcgcagca aaccgttgcc gccgcggcga
     9481 tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtgccgca ccgtggaaag
     9541 atctgccaag acgtgttttt gacgctacgc tggatatgct ttccgggcgc tatccctctg
     9601 gcgatttttc tgcttttcgc cccaaactgg tctggaaccg ggagaccggg atattgaccg
     9661 cccgacctgg cgctcaattg ttggcggtta ccagcggtgg caccattccg gatcgtggca
     9721 tgtatagcgt gttattaccc gaaggtgaag aacaggccgg ttcgcggcgg gtgggtgaac
     9781 tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc ggcgctacct
     9841 catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct ccgggtcgtt
     9901 ctgcccggct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa ttaggcgaga
     9961 tgatcggcga ttttcttcat ttgctggcgg atggcgcgtt cttttccggg actattcccc
    10021 cgtggctggc agaagaaaat acgatcgcca atattcaggg gttgattgac gagcaacgca
    10081 acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc cgtgatgaaa
    10141 ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat gaaccctggg
    10201 cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct gtggtcgcca
    10261 gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc gacgccgcga
    10321 tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta ggcagctcgg
    10381 cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg ccggggcgca
    10441 ctccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag ttgctggaaa
    10501 tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa tgtctgcaag
    10561 atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt ggcgaaattc
    10621 aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta ttgttcggct
    10681 atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg gcatccgtac
    10741 tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg ggggaattac
    10801 tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct cctggcagaa
    10861 gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca atgaccgttg
    10921 aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat ctggaaaatc
    10981 ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt cttgcctgta
    11041 tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg actaccagag tcattgccag
    11101 agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc tatctccggg
    11161 ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt attgccattg
    11221 tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg caacaagaca
    11281 tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa gccgccagag
    11341 aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa cgtcagggcg
    11401 tattacccgc caccgatggt agcccggcgc tctttgcctc aacatcgcca ggcgtttatg
    11461 agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt ttacccgcct
    11521 cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgtca gaaatgctcg
    11581 atgaattact ggcaaccggt gcggttatct ggtcggggca aaaaaactgg gtgaagatga
    11641 cggcctggtg gcactgcatc tacaggaata tgctgcagaa tcgttcactc ccgacgaagc
    11701 ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc gctgttctgg ctgacggagg
    11761 agcctggttt gcacaacaaa tcagccaacg gatacgcgac aaaatcggcg aatcggttga
    11821 tctctctgcc ctgcaagagg cgttatgggc gctggtctgg caaggcgtca tcaccagcga
    11881 catttgggca ccgttacgcg ccctcacccg cagcagttcc aacgcacgca cctcaactcg
    11941 ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg caacccgtct cgccgctggt
    12001 atcttacaac acaccaaatc tggctggatg ctggcgctgg cggaaaatat gctcgaccgc
    12061 tacggcatca tcagtcgtca ggcggtgata gccgaaaata tccctggcgg gtttccatcg
    12121 atgcaaacgc tttgtcgaag tatggaagac tccgggcgaa ttatgcgagg tcgttttgta
    12181 gaaggtctgg gtggcgcgca attcgctgaa cgtctgacta ttgaccgatt gcgcgatctg
    12241 gcgacacaag ccacgcaaac gcgccactat acaccagtgg cgctctctgc caacgatccg
    12301 gctaatgtgt ggggaaatct tctgccctgg cctgcacatc cggcaacgct ggttccaacg
    12361 cgtcgggcgg gtgcgctggt ggtcgtttct ggcggcaaat tgttactcta tctggcgcaa
    12421 ggtggcaaaa aaatgctggt ctggcaggaa aaagaggaat tactcgcccc agaggttttc
    12481 cacgcgctga ctaccgcact gcgtcgcgaa ccacggctgc gctttacgct aacagaagtg
    12541 aatgatctac cggtccggca aacgccgatg tttacgctgc tgcgtgaggc gggattttca
    12601 agttcgccac aagggctgga ttggggatag agaaaggact gacggatgcc cgttcgcatc
    12661 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
    12721 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
    12781 ccgccgacca gcttgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
    12841 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
    12901 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
    12961 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
    13021 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
    13081 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
    13141 atttgttgca tttcaaatat tcgtttcttc tctgatgccg gcaaactggc gtatttataa
    13201 ccatttttag ctttcactgc tgcatttttt tgcgctcgcc aacgaaacgt attttttaac
    13261 aataaaagct attaactttc tcttcttcta tgcattagaa tcatcaagtt ttgtaaatca
    13321 gacgcaggca tgatagacct gccttaacag agggacgctc agtggcgcgg ataaaccgta
    13381 tttcgatcac gctctgtgct ttacttttta ccaccctgcc tttaacgcct atggcccatg
    13441 cttcaaagca agccagggag agttctgcta ccactcatat caccaaaaaa gcagataaaa
    13501 agaaaagcac ggcaaccacc aaaaagaccc agaaaacagc gaaaaaagcc gccagtaaaa
    13561 gtacgaccaa aagcaaaacc gcttcttccg ttaaaaaatc ttccattacc gcttctaaaa
    13621 acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc ctcagccagc tttaccgaaa
    13681 agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa agtcaaaaat gccgcgtcag
    13741 gaactcttgc cgacgcgcac aaagcgaagg tgcaaaaagc tacgaaagtg gcaatgaata
    13801 aactgatgca gcaaattggt aagccatatc gttggggtgg cagctcaccg cgtaccggtt
    13861 ttgattgcag cggcctggtt tattacgctt ataaagattt ggtgaaaatt cgtattccgc
    13921 gtacggcgaa tgaaatgtat cacctgcgtg atgcagcgcc aatcgaacgt agtgaactga
    13981 aaaacggcga cctggtcttt ttccgtactc agggacgcgg cacagccgat catgtcggcg
    14041 tgtatgtcgg caacggcaaa tttattcagt caccgcgcac aggtcaggaa attcaaatca
    14101 cttctctcag tgaagactac tggcagcgcc actatgttgg cgctcgtcgg gtaatgaccc
    14161 caaaaacact tcgctaaaac tttaccctgt tgttacggca acagggtaag ttcatctttt
    14221 gtctcacctt ttaatttgct accctatcca tacgcacaat aaggctattg tacgtatgca
    14281 aattaataat aaaggagagt agcaatgtca ttcgaattac ctgcactacc atatgctaaa
    14341 gatgctctgg caccgcacat ttctgcggaa actatcgagt atcactacgg caagcatcat
    14401 cagacttatg tcactaacct gaacaacctg attaaaggta ccgcgtttga aggtaaatca
    14461 ctggaagaga ttattcgcag ctctgaaggt ggcgtattca acaacgcagc tcaggtctgg
    14521 aaccatactt tctactggaa ctgcctggca ccgaacgccg gtggcgaacc gactggaaaa
    14581 gtcgctgaag ctatcgccgc atcttttggc agctttgccg atttcaaagc gcagtttact
    14641 gatgcagcga tcaaaaactt tggttctggc tggacctggc tggtgaaaaa cagcgatggc
    14701 aaactggcta tcgtttcaac ctctaacgcg ggtactccgc tgaccaccga tgcgactccg
    14761 ctgctgaccg ttgatgtctg ggaacacgct tattacatcg actatcgcaa tgcacgtcct
    14821 ggctatctgg agcacttctg ggcgctggtg aactgggaat tcgtagcgaa aaatctcgct
    14881 gcataataac tgatggcaaa tgcagcattg cctgatgcgc ttcgcttacc atgccaacgc
    14941 ggatgaggcg ttaacagcgc attcggcaag ataaatcgca cgttgtcagc aactgtaacg
    15001 cagaaggtta tccttctgcg tttttgttta attagctgtt ggcaacgcaa actgtttcag
    15061 gttgttttct ggctgacata aacaccagca ataatgccag tcccgcgaca atcgccccca
    15121 tcaccggcac aaagctgtat cccagcccag cggaaattac cgcaccacca gccgctgctc
    15181 ccagcgcatt tccaagatta aaggcaccaa tattgactga tgaagacaga cctggcgctt
    15241 cactggcgac acgcatcacg cgcatctgta acggcggtac gaccgcaaag gttgctgcgc
    15301 cccacaccac catgctaata gctgcgccga actcattgcg ggccaggaac ggggttgcca
    15361 gcataatcac catcagcagc aacaaaaagc ctttcaacgt gccgttaact gaacgatctg
    15421 ccagtttgcc gccgagatag ttaccgatag agaatccgac accaatcagc accagcattg
    15481 ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag taccggagag atataggtgt
    15541 agagagtaaa cattgcgcca gctcccagta ccgtcgtcag caatgcagac agcacctgcg
    15601 gacgcattaa taccgccagc tcttttttca cttcaggtcg tgcccctgta ccacctttag
    15661 gtaatgagaa gaacagactt accattgaaa tcactcccag ccccgccgtt gccagaaatg
    15721 acatccgcca gccgatggtt tcacccaacc aggtcgccgc cggcacgcca ccgatatttg
    15781 ccagggttaa ccccataaac atagtggcaa ctgcgctggc ctgtttatgt tttggcacca
    15841 cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc gtgattcagg ctggtcaaaa
    15901 tgcgtgaaag catcagggtc atataatccg gcgcgatggc ggaaagtacg ttgccgagcg
    15961 tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc acgatgagaa agtagaagcg
    16021 tcatcagcgg cgcgcccacc attacgccaa ctgcataggc actgattaac attccggcag
    16081 cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa caagcccatt ggcgagaact
    16141 ccgttgtccc gataccaaac gcgccaatcg ccagcgccag cgccagcaac ggatagttaa
    16201 ttttcatgcc ttatctccac ctcttcgcgt cattacgcga tattcattaa agtggcgaaa
    16261 gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga gaaagcactt ttgccatttt
    16321 gctaacaaac aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca
    16381 gcgcggcagt caaacccacg gctacgatca aaccgaggac gataatcgtt gttaccagtg
    16441 aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaagtgat
    16501 attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt
    16561 tttaccactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta
    16621 ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt
    16681 gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt
    16741 agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt
    16801 ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca
    16861 acgccgtgtg ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc
    16921 tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt
    16981 ttgccgagat cattgaagca gttgaaaaaa attgcttcca gaaaggttat accctgattc
    17041 tgggcaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa
    17101 aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc
    17161 tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact
    17221 tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga
    17281 ttgaacgcgg tcaccgcgaa atcggcgtta tccccggccc gctggaacgt aacactggcg
    17341 caggccgcct tgtcggtttt atgaaggcga tggaagaagc aatgatcaag gtgccggaaa
    17401 gctggattgt gcagggtgac tttgaacctg aatccggtta tcgcgccatg cagcaaatcc
    17461 tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg
    17521 cactttgtgc tgctgatgaa atggggctgc gcgtcccgca ggatgtttcg ctaattggtt
    17581 atgataacgt gcgcaacgcc cgctatttta cgccggcgct gaccacaatc catcagccaa
    17641 aagattcgct gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg
    17701 aagaaccgca gtccattgaa gtgcatccgc gcttgattga acgccgctcc gtggctgacg
    17761 gcccgttccg cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca
    17821 gccactcctt attcagcgtc tcactatcgc cgagatactc aagcaaccag gttaacgcag
    17881 gcgacatatc attttgctgc catgtcagac aacatgccga atccggaaag gggttttcca
    17941 gttcaagtgc tacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca
    18001 tccctatgca taatcctgcc gagatacagg ttgccgatga ttcccagtca ggcacgacaa
    18061 ctcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt
    18121 cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg
    18181 ccagcgggtg atggctggca acaacgcaac tccagcttag catccccata tcccggaagg
    18241 cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat cgccagttcc acgcgcccgt
    18301 cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa gacaagaagt tcgacatcat
    18361 caaaatggcg ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat
    18421 ccactgcgat agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg
    18481 tgatctgcat ttttttgaca acagagcgcc cttctttgag aaaccacgct ccagcagcgg
    18541 tcagctccac atcacggtgc cgtcgttcaa agagcggcac cgccagccac tcttccagct
    18601 gacgcacggt atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa
    18661 aactaccatt acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtct
    18721 gcctgcaaaa tttttgaaac caatcatcaa atattaccgt ttcacaacac taatttcact
    18781 ccctacactt tgcggcggtg tttaattgag agatttagag aatatacatg caacctggga
    18841 aaagattttt agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt
    18901 atctgcctgc tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg
    18961 ccagccttag tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccgctct
    19021 ccgaccgtta tggtcgtaaa ccggtattat taatcggtat gacaattttt gcgttaggta
    19081 gtctggggat gctgtgggta gaaaacgccg ctacgctgct ggtattgcgt tttgtacagg
    19141 ctgtgggtgt ctgcgccgcg gcggttatct ggcaagcgtt agtgacggat tattatcctt
    19201 cacagaaagt taaccgaatt tttgcgacca tcataccgct ggtgggtcta tctccggccc
    19261 tggctcctct gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca
    19321 ccctgtttgc cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga
    19381 aggcccgtaa caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct
    19441 attgcggcaa cgtgctgata tacgcggcct gttcagccag tttttttgca tggctgaccg
    19501 gttcaccgtt catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg
    19561 tcccgcaaac tatcgcgttt ctgattggtg gttatggctg tcgcgccgcg ctgcagaaat
    19621 ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga
    19681 cctgggctgc gggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg
    19741 tgatggcgat tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct
    19801 tcccacacgc aactggtcgc gccgcagcgt tgcagaacac tctacaactg ggtctgtgct
    19861 tcctcgcaag tctggtagtt tcctggctta ttagtatcag cacgccattg ctcaccacca
    19921 ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg
    19981 aagaagctgg ctgccagaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat
    20041 ctatatcgat atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag
    20101 cggcctatac taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca
    20161 ggatcgttcc cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt
    20221 tctacgtcgg attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag
    20281 cgtctacgct gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc
    20341 gtgtatagaa gaagtcagtg taccggatga caactggtac cgtatcgcca acgaattact
    20401 tagccgtgcc ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc
    20461 cgattttttt aaacgcgttc tgcaagaagg ctctttgggg ttaggcgaaa gttatatgga
    20521 tggctggtgg gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct
    20581 cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt
    20641 caatctgcag agtaaaaaac gtgcctggat agtcggcaag gagcattacg atttgggtaa
    20701 tgacttgttc agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga
    20761 tgccgataat ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca
    20821 gttaaaacct gggatgcgcg tactggatat tggctgcggc tggggcggac tggcacacta
    20881 catggcatct aattatgacg taagcgtggt gggcgtcacc atttctgccg aacagcaaaa
    20941 aatggctcag gaacgctgtg aaggcctgga tgtcaccatt ttgctgcaag attatcgtga
    21001 cctgaacgac cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa
    21061 aaattacgat acctattttg cggtggtgga tcgtaatttg aaaccggaag gcatattcct
    21121 gctccatact atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaataaata
    21181 tatttttccg aacggttgcc tgccctctgt acgccagatt gctcagtcca gcgaacccca
    21241 ctttgtgatg gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg
    21301 gtatgaacga ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa
    21361 acggatgttt acctattatc tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca
    21421 gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag
    21481 gctattctat cgccccctct ccgggggcga tttcagatca ggcttctgta cctggttgat
    21541 tcatggcatt ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt
    21601 gtggatcaat ttcaatgttg acgcgtgcgc cgagtttttt cttcccaaga gtcgtgcgtt
    21661 ccagagtttc cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca
    21721 ggctaatacc gtcgatgcca ataaatcctt tgtacaggat atatttcatc aactgactat
    21781 cctggacttt aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag
    21841 cggtggtcat aatatgacct gacattaagt gtccgccaat ttcatcgctg aattttgccg
    21901 cacgctcaac gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg
    21961 tttctttcat caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc
    22021 aaccgttatg cgccacggaa gcacctgttt ccaggccgtc cagcatgtgg tcgggtaact
    22081 ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct
    22141 gtacaatccc cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca
    22201 caatagcagg tggaaaacgc ccttaccagt gaaggggtaa gaatgactat tttttcactg
    22261 gagaattaat aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc
    22321 ccattcaggc ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga
    22381 agtatatcag tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa
    22441 tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg
    22501 acatggcggc ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg
    22561 gactgctgct ggcattaacg ccggttatcg cgcaattaaa tggttccggt cgacgtgagc
    22621 gcattgcgca tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta
    22681 tgctggtgct gtggaatgca ggttacatta tccgctccat ggaaaacatc gatccggctc
    22741 tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg gggcgcgcca ggctatcttt
    22801 tcttccaggt tgcccgtaac cagtgtgaag gtctggcaaa aaccaagccg ggtatggtaa
    22861 tgggctttat cggcctgctg gtgaacattc cggtgaacta tatctttatt tatggtcatt
    22921 tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg
    22981 ttatgttcct tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatatccgta
    23041 acaaaaaagg caccgcaaaa cccgatcctg cggttatgaa acgactgatt caactcggtt
    23101 tgccgattgc gctggcacta ttctttgaag tgacactgtt tgccgtcgtg gctctgttag
    23161 tgtctccgct cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac
    23221 taatgttcgt gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc
    23281 tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgctcg gaccgggctt atggtgggtg
    23341 tctgtatggc aaccctgacg gccattttca cggtttcact gcgggagcaa atcgccctgt
    23401 tgtacaacga caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg
    23461 tatatcagat ttctgactca atccaggtga ttggcagtgg gattttgcgt ggttataaag
    23521 atacgcgttc cattttctat atcaccttta cagcttactg ggtgctgggc ttgccaagcg
    23581 gctatattct ggcactgacc gatctggtcg ttgaacctat ggggccagca ggcttctgga
    23641 taggctttat tatcggcctg acatcggcag ccattatgat gatgctgcgt atgcggttcc
    23701 tgcaacgtat gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg
    23761 caaccttcac gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc
    23821 ttcgtctggc tgtaatttca gagcgttaca cagagtttgc cgactggcat ctgttatctc
    23881 aacgcgtata tccccgttaa cttccccttt cacattgatg aagtcaggtg aaatttcttt
    23941 cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac
    24001 atgcgttgcc gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac
    24061 aatgaaagtg acgctgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg
    24121 ctgtacagta atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc
    24181 gtccagtaat gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa
    24241 attttcgtcc gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt
    24301 ttccagcgta ccggcatgca cttcagcccg cccccccaca ttcaccgaca ggttttgcgt
    24361 cttaccggaa ctgtagagct gaaggccatc cgtccccaga cgataaatgg atccattctc
    24421 aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt
    24481 atcactggct ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc
    24541 ttctccactt ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt
    24601 cccattaacc aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc
    24661 cagtacacgc aggttaccgc cattttccag caacaaaccg caggcataac cctgatcaac
    24721 gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc
    24781 gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc
    24841 attgttaccg tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc
    24901 ccccgtgacc acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt
    24961 aagttgcgtc gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat
    25021 caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg
    25081 ctacctaagt gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac
    25141 aattctgttt aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct
    25201 gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa
    25261 acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct
    25321 aatatttgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc
    25381 accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt
    25441 agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg
    25501 gacgcaccag attttcttaa tctggtcttt tcctttttcc ctctgtttat tctctgtatc
    25561 caatacgtta aaagatttac actgtcttcg tatgcgttat cagaaggaga atcgctatgg
    25621 caactttgtt acaacttcat tttgctttta atggcccatt tggcgacgca atggctgagc
    25681 agcttaaacc acttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga
    25741 cagaaagtga aaagaaccac gaagccggtg ggatctacct tttcactgat gaaaaaagcg
    25801 cccttgccta tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg
    25861 tcgccaaagt tttcgatgtc aatgagccac ttagtcaaat caatcaggca aaactcgcct
    25921 gacagaattt aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat
    25981 tctaaaaatc tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta
    26041 agtaacaatg aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt
    26101 ctcgcttcta cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc
    26161 cggtgtcggg atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc
    26221 cagtattgaa ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc
    26281 cagccatctc cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc
    26341 gcgaattctg ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg
    26401 acagcaaaaa tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac
    26461 aaatgctggc gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc
    26521 ggtgattgcc acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc
    26581 cagcccgtgg tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg
    26641 aacatccttg agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat
    26701 tgaagatgat aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat
    26761 cacgttgatg tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattcccta
    26821 cgagccctta cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggcgaata
    26881 tggccttttg gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga
    26941 ctggagccaa cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt
    27001 tgccgagcgc aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga
    27061 acgcaataaa cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga
    27121 agccgtacag gaaattgttc cacatctgaa tgaacacgac cataaacggt tcagtaaagg
    27181 ccttgcccgg gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcggct
    27241 gctggcctta cgtgaagcgg ggatcattca tattctcgcc ctcggtgaag actacaaaat
    27301 ggaaattaat gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt
    27361 ttttattgat gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct
    27421 acgcgagcaa ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt
    27481 acagcaaccc gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggttgatgca
    27541 cgaccagcct ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg
    27601 ggcggtcgta aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag
    27661 ctatcagcac tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat
    27721 attgttttca ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag
    27781 tgcggcgctt ccccctggcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt
    27841 tgaattttca ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt
    27901 tgcgccaaag ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc
    27961 caatgtacaa acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct
    28021 gcttatccgc gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat
    28081 gtgactgtta aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc
    28141 ggcataccca gcggttacga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc
    28201 gtcgctgcgg gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg
    28261 catagaggaa acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata
    28321 ctcaaccaga ctctccgccc agtccgtata tccttcctca atcagtgcat gatgaatatc
    28381 ggcataacta ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat
    28441 acgccattct cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc
    28501 gaccatgctt ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgtcc
    28561 gaagataaaa aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct
    28621 tacgccagga aacacatctc ccacggcttg cggatagaga cacaacagcc ccgtcaatag
    28681 taacaacggc agcaatccac acatgacacc aacataggcg acctgctgta agggattaaa
    28741 tttagactgt gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata
    28801 aaatcgtgtt tgttttgccg ctcgttccag ccacccctga cgacgaatgc gatagtggtg
    28861 accattatcc ccaacggcat tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa
    28921 tccgcaaact tcatgcaccg caaccagact tttaaccgca gttgcgccca ccatcgcaaa
    28981 gtgatttatc agcccacttg ccagcaacaa tacaaagagt aacgcattcg accagtgcca
    29041 taatctgacc gctttgctgt aaagataaac cttctcacca tgatcggtgg ctgatttctt
    29101 cacgccacga gcacgaagca aggcgtgcag ccccaacacc aaccacatcc caaccagcaa
    29161 tactccggca atgatcaacc acaccggcca aaattcggga gtgaactgtg gcacatagtt
    29221 cgctaactgg ctctgaaact gttcagcatg ttgcgacggg ttcatacatt ttccttttta
    29281 atcaaatgtt gaccgaaccg gcgatacaga tgcggtttcc cggcaccaag tagctgatat
    29341 tgatagtatt tattttcctg caaccacgcc tgaatttctg ggctatcttc gcgaccaaag
    29401 atcaatgcgt gttccggaca ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg
    29461 gactcagcgc agaaatcaca tttatctgcc actttagtga cagggttaag gtagcgaacc
    29521 tggtacggac aagcgccaat gcaataactg cagccgatgc actgcgattt ttctacccgc
    29581 acgatcccct gctcatcacg ccacgacgcc cctgtcgggc atacgtcgat gcacggagca
    29641 tcttcgcagt gctgacatga ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg
    29701 gtgactggaa tgtgtgctat cgataaacgg cttccctgag cggggacgtg gttagtttta
    29761 cggcatgcgc gggcgcaaat attacagcca ttgcaccgtg actcgtcatg aatcatggca
    29821 taccgaactt ccttttcttg cctcgtgttc gctaacagag aagacgcaga accagtaaaa
    29881 aagattactg ttcccatccc cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc
    29941 cctaatgcag tggctgcaac tgtccaccct atccatgaca tctccctgaa atttcagggg
    30001 ttctgaaact gctctacctc gaaccattcg cataagccat aagccttgcc ataggtcata
    30061 aatcgctcgc taaacaagta caacaccgcc gtcgatgact ccacctgccg tatatcggca
    30121 tattcaggtg caacatccat cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa
    30181 tatggcgctt gcatcagcat ggcgactttg taaggacgag gatacgtctg gcactcaaaa
    30241 cgaacggcgt gtgcaatggc gcgacaaatg tcctgctcca caacttgcaa agacatcgcc
    30301 gcgtagtttt cactcatggc ctgcgttgag taatagtaat catcctgact tcccgtcata
    30361 caagcgatat ccgcgtagtc tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc
    30421 tcttctgcaa ccgaataggg tggcgagaga aacacggtgc gcgccaccag ctgcccagcc
    30481 gcagaatgct ggcgaatgaa ctcagccagg atttcagcag gttccggttc ggttaccgta
    30541 tcaacggggt gacgcatccc ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact
    30601 ttcgctaacg gcaattcatc ctgatggttc atcttaccca ttccttacgc aggcagtaga
    30661 ttgtgtgctg ccagatcggc ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg
    30721 gttggacaac caagctgtgg gtcccagccc atttctttgt agaacatcgt gagcgaagca
    30781 tgcatatcgt cacgatccat tttgtcagta ccttcagtaa agaccgggat ctgcggatcc
    30841 ttgtcgaaaa cccaggaaca gataagatcg tgttcattac gcatatcttt ggtttgcatc
    30901 agttttaccg tgtaggcacg atgcaacgta aagatacgct ctgcagctaa atctaatttt
    30961 tcctgagtca tctcttcgcc ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg
    31021 ccccggtaat tacggctttt cagcggcgaa acggtcattg gccagaccca gttgcacagg
    31081 gtgacggcgt tatgcaaaca gacccgcaac agcgaccact ttgcatattt aatttttgcg
    31141 tcattgattg gcgtgtagtt tttggtttca tcgtaagcat cttcagaacc aaaaagttct
    31201 ttcgccactt cacgttgcag tttcaatggc aagccggaac caataaagtt gatatgggta
    31261 tgcgtcatgc agtcacggtt gaacatacag ttaacaatgg aaccgacctg cgctgacgct
    31321 tcattggcat ggtgaaccgg atagccaaac ggcgaccaga gtttattttt cgcgtagccc
    31381 cagtactctt cacccaaatt ccagcgttct gcgatggcat atgaaccatc agccaggtga
    31441 ctcagctcac ccacacgatg cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca
    31501 cccgcttcca gttgatccca gcgaatttcc gcatactctt cagcaggcag aacacgcttg
    31561 aacacacctt tgctgtaaca ataggtaaag tcgcgatgca actgcccgta gttacaccat
    31621 aggccgtagt catcgaacag attcagaccc accaggttac caatcacacg accatcgtct
    31681 ttatcttcaa aatctttcgg accgttcggg aagatggtgg tatggacaaa gttggctaca
    31741 caagtgttac cacctgtgct gggcacgcca aactctttca cccgaggaat attcatttgg
    31801 gtcatacaac ggatcgggca agagtggcag ccactcattt ttactgtgta tttctctgcc
    31861 gccggaccta agtcaaaaac ggatttatag gtacgaaagc cgaccgtgtt ctgattgcct
    31921 ggcggaattt cccccgtttc aatcggacca ccttcagccg cgccccaaaa cagcccttta
    31981 cgtgctgtcc aacgtgactt gggatctgaa tactctgccc acgattgcgg cgtacttggc
    32041 acgacatggt tgttattcgc accaataagt tcagtcatca tgtaatcgtt gagacgcttc
    32101 atctcctgac gatcggcaat gttgacccct ttcgtccctt caaccgcaat cgctttcagg
    32161 tttttcgaac ccattattgc gccagttccc gcaccgccgc tgtggttacg gctattcaac
    32221 atgccagaga gaggaacaag gttttcccca gcctgaccaa tagccgccac acaggtttcc
    32281 ggactggtca atcgacaaat ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa
    32341 tcggcttttt ccaggctaac tttgtcatct ttaatcttca gccataccgg tgatttcgcc
    32401 ttcccttcga taataatgac gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca
    32461 cccatatggg catcgacgac taaatttcct ttggtaaaag tagaaagtga ggtgatattt
    32521 acgcgagaac tacagggggc accagatcca gttaatgggc cggtagcaaa gactaattta
    32581 ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt catacataat tttgtagccg
    32641 aagcccatgc caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct
    32701 gtcgtgagat tgactcttaa tatattacct gtccaaccgt tagccatgat tttttccttt
    32761 gcaagattca cacagtaata tctttccact cgataatttt taacgcccct gttgggcagg
    32821 cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg gtatttacgg
    32881 tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacag cgtttatgat
    32941 cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg cagacgttca
    33001 tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga tcgccataca
    33061 ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg attttgatac
    33121 gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt tcacagcggt
    33181 ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta tttatcaacc
    33241 ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg
    33301 caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat cgcgttaagc
    33361 caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct tcgcaaacac
    33421 agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc tgactctgtc
    33481 cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta acgccgataa
    33541 accaatcctt tatggttagg agaaa
//
LOCUS       DAZPLM010000019        33489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPLM010000019 DAZPLM010000000
VERSION     DAZPLM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.019"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            21..722
                     /locus_tag="ACHVC7_000801"
     CDS             21..722
                     /locus_tag="ACHVC7_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2114922.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            747..3346
                     /locus_tag="ACHVC7_000802"
                     /pseudo
     CDS             747..3346
                     /locus_tag="ACHVC7_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3337..4407
                     /locus_tag="ACHVC7_000803"
     CDS             3337..4407
                     /locus_tag="ACHVC7_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114923.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4419..4961
                     /locus_tag="ACHVC7_000804"
     CDS             4419..4961
                     /locus_tag="ACHVC7_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114924.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4969..5484
                     /locus_tag="ACHVC7_000805"
     CDS             4969..5484
                     /locus_tag="ACHVC7_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2114925.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5450..6187
                     /locus_tag="ACHVC7_000806"
     CDS             5450..6187
                     /locus_tag="ACHVC7_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR2114926.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6298..7308
                     /gene="pyrD"
                     /locus_tag="ACHVC7_000807"
     CDS             6298..7308
                     /gene="pyrD"
                     /locus_tag="ACHVC7_000807"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR2114927.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7482..8024
                     /gene="zapC"
                     /locus_tag="ACHVC7_000808"
     CDS             7482..8024
                     /gene="zapC"
                     /locus_tag="ACHVC7_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR2114928.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8021..9130)
                     /gene="ycbX"
                     /locus_tag="ACHVC7_000809"
     CDS             complement(8021..9130)
                     /gene="ycbX"
                     /locus_tag="ACHVC7_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR2114929.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9374..11482
                     /gene="rlmKL"
                     /locus_tag="ACHVC7_000810"
     CDS             9374..11482
                     /gene="rlmKL"
                     /locus_tag="ACHVC7_000810"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR2114930.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11494..13401
                     /locus_tag="ACHVC7_000811"
     CDS             11494..13401
                     /locus_tag="ACHVC7_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2114931.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13531..14784
                     /gene="pqiA"
                     /locus_tag="ACHVC7_000812"
     CDS             13531..14784
                     /gene="pqiA"
                     /locus_tag="ACHVC7_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR2114932.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14789..16429
                     /gene="pqiB"
                     /locus_tag="ACHVC7_000813"
     CDS             14789..16429
                     /gene="pqiB"
                     /locus_tag="ACHVC7_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR2114933.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16426..16989
                     /gene="pqiC"
                     /locus_tag="ACHVC7_000814"
     CDS             16426..16989
                     /gene="pqiC"
                     /locus_tag="ACHVC7_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR2114934.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17245..17412
                     /gene="rmf"
                     /locus_tag="ACHVC7_000815"
     CDS             17245..17412
                     /gene="rmf"
                     /locus_tag="ACHVC7_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR2114935.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17482..18000)
                     /gene="fabA"
                     /locus_tag="ACHVC7_000816"
     CDS             complement(17482..18000)
                     /gene="fabA"
                     /locus_tag="ACHVC7_000816"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR2114936.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18069..19829)
                     /locus_tag="ACHVC7_000817"
     CDS             complement(18069..19829)
                     /locus_tag="ACHVC7_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2114937.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20015..20467
                     /gene="matP"
                     /locus_tag="ACHVC7_000818"
     CDS             20015..20467
                     /gene="matP"
                     /locus_tag="ACHVC7_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR2114938.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20543..21583)
                     /gene="ompA"
                     /locus_tag="ACHVC7_000819"
     CDS             complement(20543..21583)
                     /gene="ompA"
                     /locus_tag="ACHVC7_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR2114939.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21938..22447)
                     /gene="sulA"
                     /locus_tag="ACHVC7_000820"
     CDS             complement(21938..22447)
                     /gene="sulA"
                     /locus_tag="ACHVC7_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR2114940.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22666..23295
                     /gene="sxy"
                     /locus_tag="ACHVC7_000821"
     CDS             22666..23295
                     /gene="sxy"
                     /locus_tag="ACHVC7_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR2114941.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23258..25420)
                     /gene="yccS"
                     /locus_tag="ACHVC7_000822"
     CDS             complement(23258..25420)
                     /gene="yccS"
                     /locus_tag="ACHVC7_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR2114942.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25430..25876)
                     /locus_tag="ACHVC7_000823"
     CDS             complement(25430..25876)
                     /locus_tag="ACHVC7_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR2114943.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25999..28053
                     /gene="helD"
                     /locus_tag="ACHVC7_000824"
     CDS             25999..28053
                     /gene="helD"
                     /locus_tag="ACHVC7_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR2114944.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28085..28543)
                     /gene="mgsA"
                     /locus_tag="ACHVC7_000825"
     CDS             complement(28085..28543)
                     /gene="mgsA"
                     /locus_tag="ACHVC7_000825"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR2114945.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28639..29301)
                     /locus_tag="ACHVC7_000826"
     CDS             complement(28639..29301)
                     /locus_tag="ACHVC7_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR2114946.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29474..29887
                     /locus_tag="ACHVC7_000827"
     CDS             29474..29887
                     /locus_tag="ACHVC7_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR2114947.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29932..30249)
                     /gene="hspQ"
                     /locus_tag="ACHVC7_000828"
     CDS             complement(29932..30249)
                     /gene="hspQ"
                     /locus_tag="ACHVC7_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR2114948.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30307..31497)
                     /gene="rlmI"
                     /locus_tag="ACHVC7_000829"
                     /pseudo
     CDS             complement(30307..31497)
                     /gene="rlmI"
                     /locus_tag="ACHVC7_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            31592..31870
                     /gene="yccX"
                     /locus_tag="ACHVC7_000830"
     CDS             31592..31870
                     /gene="yccX"
                     /locus_tag="ACHVC7_000830"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR2114949.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31867..32196)
                     /gene="tusE"
                     /locus_tag="ACHVC7_000831"
     CDS             complement(31867..32196)
                     /gene="tusE"
                     /locus_tag="ACHVC7_000831"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR2114950.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32287..32946)
                     /gene="yccA"
                     /locus_tag="ACHVC7_000832"
     CDS             complement(32287..32946)
                     /gene="yccA"
                     /locus_tag="ACHVC7_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR2114951.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33153..33240)
                     /locus_tag="ACHVC7_000833"
     tRNA            complement(33153..33240)
                     /locus_tag="ACHVC7_000833"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33204..33206),aa:Ser,seq:tga)
BASE COUNT         8639 a         8006 c         8753 g         8091 t
ORIGIN      
        1 ttttttaaga gtgactattt atgaaaactt gcataacaaa gggaattgtg accgtaagtt
       61 taacggcaat attactcagc tgctcatccg catgggcggc aggtaaaggc gggattggac
      121 ttgcagcaac acgtcttgtt tattcagaag gtgaagagca aatttcactg ggtgtacgta
      181 acaccagtcc ggatgttcct tatcttattc agtcatgggt gatgacccca gataataaaa
      241 aatcagcaga ttttattatt acaccaccgc tatttgtgct gaatccggct aatgagaatc
      301 tgttacgcat tatgtacatt ggtgcgccgt tggcgaaaga cagagaaacc cttttcttca
      361 ctagcgtacg ggcagtccct tcaacaacga agcgggaaga gggaaatacc ctgaagattg
      421 ccacacaaag cgtcatcaaa cttttctggc gaccaaaagg tttagcgtat cccttaggcg
      481 aggctccggc gaaactgcgt tgcacttcgt cagctgacat ggttacggtc agtaacccaa
      541 caccttattt cattaccctg acagacctga aaataggggg aaaggtagtt aaaaatcaaa
      601 tgatttcccc ctttgataaa taccaattct ctctgccaaa gggggccaaa aatagcagcg
      661 taacgtatcg aaccatcaat gattacgggg cggaaacgcc acaactcaac tgtaaatcgt
      721 aagccgtctt cagttaagag agcgagatgt atagaactca tcgacaacac agcctgttaa
      781 gctctggtgg agtgccatcg tttattggtg ggctggtggt gtttgtgtcg gcagcgttca
      841 atgcacaagc tgaaacctgg ttcgatcctg cttttttcaa agatgatccc tcaatggtgg
      901 ccgatttgtc tcgtttcgaa aaaggacaaa aaataacgcc aggggtttat cgtgtcgata
      961 ttgttctgaa tcagacaatt gtagatacgc gcaacgtcaa ttttgttgag ataacgccag
     1021 agaaggggat tgccgcctgt ttgacgactg aaagcctgga tgcaatgggt gttaatactg
     1081 atgcgtttcc ggcttttaaa caactggaca aacaagcgtg tgtgccattg gcggagatta
     1141 ttccggatgc cagcgtaacg tttaatgtga ataaactccg tctggaaatt tcagtaccgc
     1201 aaatcgccat caaaagtaat gcccgtggtt atgtcccccc tgaacgttgg gatgaaggga
     1261 tcaacgcgct attactggga tattcgttta gcggggctaa cagtattcat agcagcgcag
     1321 acagtgattc tggcgacagc tattttctga atttaaacag tggcgttaat ttaggcccat
     1381 ggagattgcg caacaattca acatggagtc gcagtagtgg ccaaaccgca gaatggaaga
     1441 atctcagcag ctatttgcag cgggcggtta ttccactaaa aggcgaactg accgtaggtg
     1501 atgattatac tgcaggcgat tttttcgata gtgtcagctt tcgtggtgtg cagctggcgt
     1561 cagatgacaa catgctgcca gacagcctga aagggtttgc gcctgtggtg cgtggtatcg
     1621 ccaaaagcaa tgcccagata acgattaagc aaaatggtta caccatttac caaacttatg
     1681 tatcgcctgg tgcttttgaa attagtgatc tctattccac gtcgtcgagc ggtgatttgt
     1741 tagttgaaat caaagaagcg gacggcagcg tcaatagcta cagcgtaccg ttttccagcg
     1801 tgccattact ccagcgtcag gggcgaatca aatacgcggt gacactggcg aaatacagaa
     1861 ccaatagtaa tgaacagcag gagagcaaat ttgcctaggc cacgttgcag tggggcggac
     1921 cgtggggaac gacatggtat ggtggtggac aatatgctga atattaccgt gccgccatgt
     1981 ttggtctggg atttaacctt ggcgatttcg gagcaatttc gttcgatgcg acccaggcga
     2041 agagtacgct ggcagaccaa agcgaacata aaggtcagtc atatcgtttt ctgtatgcca
     2101 aaacgctcaa ccaattgggc actaattttc aattgatggg ctatcgctat tcgacgtcgg
     2161 gtttctacac cctttccgac accatgtata aacatatgga tggctacgaa tttaatgacg
     2221 gtgatgatga agatacgccg atgtggtcgc gttattacaa tttgttttac accaaacgtg
     2281 gcaaactgca ggtcaatatc tcccagcaat taggcgagta cggttcgttt tatttaagtg
     2341 gtagccagca aacttactgg cataccgatc aacaggatcg gctattacag tttggctaca
     2401 acacgcaaat taaagatctc tcgctggggg gttcctggaa ctacagtaag tcccgtggtc
     2461 aacctgatgc tgatcaggtg tttgcactaa atttttccct gccgctcaat ctgttgctcc
     2521 ccagaagtaa tgatagctat accaggaaaa aaattacgcc tggatgacct ctaacaccag
     2581 catcgataac gaagggcaca ttacacaaaa cctgggttta acggagacac tactcgatga
     2641 cggtaacctg agctacagcg tgcaacaggg atataacagc gaggggaaaa cggctaatgg
     2701 tagcgccagc atggactaca aaggggcgtt tgcagatgcc cgagtgggct acaactacag
     2761 cgataacggc agtcaacaac aactgaacta cgctctttca ggcagtttag ttgcccattc
     2821 acagggcatt accctggggc aatcgctggg ggaaactaac gtgctgattg cagcaccagg
     2881 cgcagagaat actcgtgtgg cgaacagcac cgggctgaaa actgactggc gcggatatac
     2941 cgttgtcccc tatgccactt cttatcggga aaatcgaatc gcacttgatg cggcatcgtt
     3001 aaaacgtaac gtggatcttg aaaatgcagt agtcaacgtg gttcccacca aaggggcgtt
     3061 ggttctggcg gagttcaatg cccatgcggg agcaagggta ttaatgaaaa catcaaagca
     3121 gggtataccg ctgcgttttg gcgcgatagc gacgctggac ggcgtacaga ctaatagcgg
     3181 cattattgat gatgatggct cgctctatat gtccggtttg cccgctcagg gagcgataac
     3241 tgtacgctgg ggcgaagctc ccgatcaaat ttgtcatatc agttaccagc ttaccgaaca
     3301 acaaattaac tctgcgatta cgcgaatgga tgccatatgc agataatctt tggagaaaaa
     3361 tgcgtgttat tactacgact attttttgcc gccgtcttaa tgctatggtg cgctcaaact
     3421 gctgcttata gcgggcagtg tcataccact caggggaatc cgtatattgg cgtcaatttt
     3481 ggcgttaaaa ccctggagga agaagaaaat acggctggag tagttaaaga caaattttat
     3541 cagtggaacg aatcgaatga ttattatgtt tcctgtgatt gcgataaaga caatgtcaga
     3601 agtggccgat gggcattcgc cgcggattca ccgttagtct atttaggcga caactggtac
     3661 aaaattaatg actatcttgc cgccaaagtt ttattgcagg ttaaaggcag ttctcctact
     3721 gcggttcctt tcgaaaacgt gggcacaggg gcagatacac gatggcatat ttgcgatccc
     3781 ggcggtcaac gtttaggtgg ccagggggct agcggtaata gcggtagctt ttccctgaaa
     3841 atattgcagc cgttcgttgg ttcggtcgtc attcctccta tggcgctggc gcgattattt
     3901 gaatgctaca acatacccgc aggtgattcc tgcacgacta caggtacacc ggttttagtg
     3961 tattacctgt ctggtacgat caattcactt ggctcatgtt ccgtcaatgc cggagaaaca
     4021 atcgaggtcg atctgggcga cgcatttgcg gctaactttc gtgttgtagg gcataagcct
     4081 cttggggcca gaacggcaga acttgcaatt ccagtcaggt gtaacacggg aaacgcgggg
     4141 ttagttaacg tcaacctgag tctgacggca accacagacc ccagctatcc ccaggcgatt
     4201 aagacgtcac gtcctggcgt gggcgtggtg gtgaccgata gccagaacaa cattatttcc
     4261 cctgctggtg gaacattacc gctctctatt cctgatgatg cagacagtat cgcgcgaatg
     4321 aatgtctatc cagtcagcac gacaggtgta ccaccagaaa ccgggcgatt tgaagccacg
     4381 gcaacggtga gaataaattt tgattaacgc gtgaacgtat gaagaaaaaa acgatatttc
     4441 agtgcgttat cttgttcttt agcattctta acatccatgt cgggatggct gggcctgaac
     4501 aagttagtat gcatatttat gggaatgtgg tcgatcaggg ctgtgatgtc gccaccaaaa
     4561 gtgcattaca aaatattcat attggtgatt ttaatatcag tgattttcag gccgcgaata
     4621 ccgtaagcac tgctgctgat ttgaatatcg atatcaccgg ttgtgccgct agtattactg
     4681 gcgcggacgt cctttttagc ggcgaggctg acacccttac gccgacactg ctcaaactaa
     4741 ctgacacagg cggaagcggt ggtatggcaa cggggattgc cgtgcaaatt cttgatgcgc
     4801 aaagtcagca agaaatcccg ctcaatcagg tccagcctct tacgccctta aaagccgggg
     4861 ataacacact caaatatcaa cttcgttata agtccacaaa ggcgggagca acgggcggta
     4921 atgcgacggc ggttctctat tttgatctgg tttaccagtg aaggagtgat gttgaaacgg
     4981 ataatctgga ttctgttctt actgggatta acggggggct gtgagctatt tgcccatgat
     5041 ggcacggtca acattagcgg atcgtttcgc cgtaatacat gcgtgctggc acaggatagc
     5101 aagcaaatca atgtgcagtt aggcgatgtc tcgctgacac gtttttttca tgggaattat
     5161 ggcccggaga aatctttcat cattaatttg caggattgcg gaacagatgt cagcacggtt
     5221 gacgtcacct tttcaggaac ccccgacgga gtgcagagcg aaatgctgtc cattgaaagt
     5281 ggtacggatg cagccagcgg gctggcaatt gcgattctgg atgatgcgaa gatattgatt
     5341 ccgcttaacc aggcgagtaa agactacagc ctacatagcg gtaaggtgcc attaactttt
     5401 tatgcgcaat tgcgacctgt caatagcgat gtgcagtcgg gtaaagtgaa tgccagcgcg
     5461 acatttgtac ttcattatga ctaatacgtg gaacagattg gcgctcttga ttttcgccgt
     5521 tttatcgctg ctggtggcgg gtgaattgcg ggcaggagtg gtggtcggcg gaacgcgatt
     5581 tatctttccg gcagacagag aatcgatatc tattttactg actaatacca gtcaggaatc
     5641 ctggcttatt aacagtaaaa tcaaccgccc aacgcgttgg gccggggggg aagcgtcgac
     5701 agtgccagca ccattactgg ccgctccgcc acttattctc ctgaagcccg gtactacagg
     5761 cacgttgcgc ttgctgagaa cggaaagcga catcttgcct gtggatcgcg aaacgctatt
     5821 tgagttaagc attgccagcg tgccatccgg caaagttgaa aatcagagcg taaaagtggc
     5881 gatgcgctcg gtatttaaac tgttctggcg acccgaaggt ttgccgggcg acccgctgga
     5941 agcttatcaa caattacgct ggacacggaa ttcgcagggt gtacaactca ctaacccaac
     6001 gccttactac attaacctga ttcaggtgag cgtaaatggt aaagcgttaa gtaatgcggg
     6061 agttgtgccg cctaaaagcc agcgtcagac aagctggtgt caggcaatcg caccgtgtca
     6121 tgtcgcctgg cgggcgataa atgattacgg cgggttgagt gcaaagaagg agcaaaatct
     6181 gccctgaaac aggttcggaa aacgtttgcg ttttttttgc cgcaggtcaa ttcccttttg
     6241 gtccgaactc gcacataata cgcccccggt ttgcacaccg ggaatccagg agagttcatg
     6301 tactacccct tcgttcgtaa agcccttttc cagctcgatc cagagcgcgc tcatgagttt
     6361 acttttcagc aattacgccg tattacagga acgccgtttg aagcactggt gcggcagaaa
     6421 gtgcctgcga aacctgttaa ctgcatgggc ctgacgttta aaaatccgct tggtctggca
     6481 gccggtcttg ataaagacgg ggagtgcatt gacgcgttag gcgcgatggg atttggatcg
     6541 atcgagatcg gtaccgtcac gccacgtcca cagccaggta atgacaagcc gcgtctcttt
     6601 cgtctggtag atgccgaagg tttgatcaac cgtatgggct ttaataatct tggcgttgat
     6661 aacctcgtag agaacgtaaa aaaggcccat tatgacggcg tcctgggtat taacatcggc
     6721 aaaaataaag atacgccagt ggagcagggc aaagatgact atctgatttg tatggaaaaa
     6781 atctatgcct atgcgggata tatcgccatc aatatttcat cgccgaatac cccaggatta
     6841 cgcacgctgc aatatggtga agcgctggat gatctcttaa ccgcgattaa aaataagcaa
     6901 aatgatttgc aagcgatgca ccataaatat gtgccgatcg cagtgaagat cgcgccggat
     6961 ctttctgaag aagaattgat ccaggttgcc gatagtttag ttcgccataa tattgatggc
     7021 gttattgcaa ccaataccac actcgatcgt tctcttgttc agggaatgaa aaattgcgat
     7081 caaaccggtg gcttaagtgg tcgtccgctt cagttaaaaa gcaccgaaat tattcgccgc
     7141 ttgtcactgg aattaaacgg tcgcttaccg atcatcggtg ttggcggcat cgactcggtt
     7201 atcgctgcgc gtgaaaagat tgctgcgggt gcctcactgg tgcaaattta ttctggtttt
     7261 atttttaaag gtccgccgct gattaaagaa atcgttaccc atatctaatt atttcttcga
     7321 attcctcttt ataagataat cagggcttta ttttcagccc tggttgtttt atattcatcg
     7381 ctgttgctta tttagacatt ttgtactttt attgttgagg ttattaagcg aagcgacaat
     7441 ggattgtgtt gctgcggttt tatagggtag gggagaggca gatgcgaatt aaaccagacg
     7501 ataactggcg ttggtattac gatgaagagc acgatcgaat gatgctcgat ttagccaatg
     7561 gtatgctatt tcgctcacgt tttgcgcgca agatgttgac tccagatgcc ttttctcccg
     7621 caggcttttg cgttgacgac gccgcgctct atttctcttt tgaagaaaag tgccgcgatt
     7681 ttaatttatc taaagaacaa aaagccgaac tggtgctgaa tgcactggta gcaattcgct
     7741 atctcaaacc gcaaatgccg aaaagctggc attttgtttc ccatggtgaa atgtgggtgc
     7801 ccatgccggg cgatgcggcc tgcgtttggt tgagcgatac tcacgagcag gtcaatttgc
     7861 tggtcgttga atctggcgaa aatgccgcac tatgcctgct ggcacaaccc tgcgttgtta
     7921 ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat catgaacgac aggctgaaac
     7981 cgcaggttaa tgttgacagc ttcagcctcg aacaggcagt ctaacgcgcc aacttaagtg
     8041 cagtcttcgg tacacagcta cagcaaagaa tggtgccatc atcgcccatt gctgattttt
     8101 tcagcggcgt gacttcgcct tctaaaagct gaacacggca acttccacaa atgcccgcgc
     8161 ggcaagaata agggatacga attccctgat tttctaattg ctccagcaac acctgttggt
     8221 tatttccacg aaatgcctgt ccctgccagt caatatctac atttgcgtcc ggttgttgcg
     8281 tgatgttggc ggtatcatca gcggcagctg cgccgtaaat ttttgccgga gccgttgcca
     8341 gaatttccac ctcatcgcca acgcgaatca cgccgctatt acgggcaatt aaattctgac
     8401 caaaatcgac atcgccgtta tcctgggcag tgcggaaaga ttgcaatgtt tttaatggtt
     8461 cgcctgccgg atgtttttgc cctttttctg ggctgacggt ggtgaaaata cagcggctac
     8521 aaggtttaac cacatcaaac accacatcac caatgcgaat cactttccag cgatcttctt
     8581 cccaggctga cgcgccggaa accaccagat tggggcggaa ttgctccatt tttacactgg
     8641 ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt agcaagaagg taaggatagc
     8701 catcagcaaa tgacagaggt acagtgttgt ggcgtttcac gcgccgggtc atttgtggcc
     8761 ccacccagcg taattgcact tcgcgggaga aaaatccact tagccatttg ttgatcgcgt
     8821 ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc ggttggtgcg tcttgtgtgg
     8881 cgaaatcagc aaaacgaaca tatgcgctac tgccatctgg tgcggtgaga tgcaagccat
     8941 catgcacagg tgaaggagta aaccgtacca tctggggaaa ctggcgagcg gtaataaacg
     9001 taccgtcagg ttccgtgatc ataaagatgc gatcgaaggc cagaccactg acatcagcca
     9061 gagcatgtgt aagaccaatg ccgcgcatcg atttaacagg atgaataaaa agccgggtta
     9121 atgtcgccac ggtgcggtcc tcaaatgaaa ataagccctc aactttatga catgacgcgc
     9181 ttattagcta taatgcgcaa caattttctt agcgcctgtc ccattaggct attttatttg
     9241 tcagtttggc cctgggttgt gctcgaaatc ctcacgtact tgtgtacgct ccggtttctc
     9301 cgcgctgtcc atgtccagac tgactgcaac aattacgcct actgcggtag gctcttagag
     9361 taaaagtgac gatatgaatt ctctgtttgc cagtacggcc cgtgggctgg aagagctgtt
     9421 aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag gtggttcagg gtggggtcca
     9481 tttcaagggc gacacacggc ttgtttacca gagcctgatg tggagccgcc tggcctcgcg
     9541 tattatgttg ccgctgggcg agtgtaaggt ttacagcgat ttagacctct atctcggtgt
     9601 tcaggcgatc aactggacag agatgtttaa tcttggcgcg accttcgctg tccacttcag
     9661 tggtttgaat gacaccatcc gcaacagtca gtacggcgcg atgaaagtga aagacgcgat
     9721 cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat gttgatcgcg atgcgccgga
     9781 tatccgcgtt aacgtctggc tgcataaaga aaccgccagt atcgctcttg atctgagtgg
     9841 tgatggttta catctgcgtg gctatcgcga tcgtgctggt attgcgccga tcaaagaaac
     9901 cctggcagcc gctattgtga tgcgaaccgg ctggcagcca ggaacaccgc tgctcgatcc
     9961 gatgtgtggt tccggtacgt tgctgattga agcggcgatg ctagcgaccg atcgcgcacc
    10021 aggcttgcac cgtgggcgtt ggggctttag cggctgggtg cagcatgatg aagctatctg
    10081 gcaggaagtg aaagcggaag cgcaaactcg tgcccgtaaa ggcctggctg agtatagctc
    10141 tcatttttac ggttcggaca gcgacgcacg ggtgattcaa cgtgcgcgca ctaacgcccg
    10201 tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa gatgtcgcgc aactgaccaa
    10261 tccgctgccg aaagggccgt acggcacagt gttgagcaac ccgccatacg gggaacgtct
    10321 ggacagcgaa ccggcgctga ttgcgctgca tagcctgctg ggtcggatca tgaaaaacca
    10381 gtttggtggc tggaatctct ctttgtttag tgcctcgccg gatctgctaa gctgcttgca
    10441 gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg ctggactgcg tacagaaaaa
    10501 ttaccacgtt gccgaaagca ctccagacag caaaccggcg atggtagcgg aagactacgc
    10561 caaccgcctg cgtaagaacc tcaaaaaatt cgagaagtgg gctcgccagg aaggaattga
    10621 atgttaccgc ctgtatgacg ccgatctacc ggaatataac gttgccgttg accgttatgc
    10681 cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact attgatgctc acaaagcgcg
    10741 tcagcgtctg ttcgatatta tcgctgcaac catttcggta ctggggattg cgccaaacaa
    10801 actggtgctg aaaacccgtg aacgccagaa gggcaaaaac cagtaccaga aactgggcga
    10861 gaagggcgag tttcttgaag ttaccgaata taacgctcac ttgtgggtga acctgacgga
    10921 ttatctcgat actggtctgt tcctcgatca ccgtatcgcc cgccgtatgc tcggtcagat
    10981 gagcaaaggc aaagatttcc tcaacctgtt ctcttatacc ggtagcgcca ccgtgcatgc
    11041 gggattaggc ggcgcgcgca gcaccaccac cgtggatatg tcgcgtactt atctggagtg
    11101 ggcagaacgc aacctgcgtc tgaatggcct gaccgggcgt gcgcatcgcc tgattcaggc
    11161 cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttt gatctgatct ttatcgatcc
    11221 gccaaccttc tctaactcaa aacgaatgga agatgcgttt gatgttcagc gcgatcatat
    11281 ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt gggacgatca tgttctcgaa
    11341 caacaaacgt ggcttccgta tggatctcga cggcctggca aaactgggac tgaaagcaca
    11401 agaaattacg caaaaaacgc tctcccagga tttcgcccgt aaccgccaga tccacaactg
    11461 ctggctgatt accgcagcct gaaaggaata gtaatgtcat taatcagtat gcatggcgca
    11521 tggctgtcgt tcagcgacgc gccgcttctc gataacgcag aactgcatat cgaagataac
    11581 gaacgtgttt gtctggtggg tcgcaacggc gcgggcaaat cgacgttaat gaaaatcctc
    11641 aaccgtgaac aagggctgga tgacggtcgc attatttacg agcaagattt gattgtagct
    11701 cgtctgcaac aggatccgcc gcgtaacgtt gagggtagcg tttatgattt cgttgccgaa
    11761 ggcattgaag aacaagcgga atatctgaaa cgctatcacg atatttcgcg cctggtgatg
    11821 aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg ttcaggaaca gttggatcac
    11881 cacaacctgt ggcagctgga aaaccgcatc aacgaagtgc tggcgcaact ggggttagat
    11941 cctaatgttg cgctgtcgtc gctttccggc ggctggttgc gtaaagcggc attaggacgc
    12001 gcgctggtga gtaatccgcg cgtgctgttg cttgatgaac cgacaaacca cctggatatt
    12061 gaaaccatcg actggctgga agggttcctt aaaaccttca atgggacgat tattttcatc
    12121 tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca ttgttgatct cgatcgcggc
    12181 aagctggtga cctatccagg gaattacgac cagtacctgc ttgaaaaaga agaagccctg
    12241 cgcgtggaag aactacaaaa tgccgagttc gatcgcaaac tggcgcagga agaggtgtgg
    12301 atccgccagg ggatcaaagc acgccgtacc cgtaatgaag gccgcgtacg cgccctgaaa
    12361 gcaatgcgtc gcgagcgtgg tgaacgtcgc gaagtgatgg gtaccgcaaa gatgcaggtg
    12421 gaagaggcca gccgctccgg taagatcgtt ttcgaaatgg aagacgtttg ctaccaggtt
    12481 gacggtaagc aactggtgaa agatttttcg gcccaggttc tacgtggcga caaaattgcc
    12541 ctgattggtc cgaatgggtg cggcaaaacc acgctgctga aactgatgct cggtcagctt
    12601 caagcggaca gcgggcgtat tcacgttggc accaaactgg aagtggctta tttcgatcag
    12661 caccgcgcgg aactggatcc cgataaaacg gtgatggata accttgccga aggtaagcaa
    12721 gaggtgatgg ttaacggcaa gccacgccac gtattgggct atttgcagga ctttctgttc
    12781 catccgaaac gggcgatgac gccggtacgt gcgctttctg gcggtgagcg gaaccgcttg
    12841 ctgctggcgc gtttgttcct caaaccaagc aacttattga ttcttgacga accgaccaac
    12901 gatcttgatg tcgaaacgct ggaactgctg gaagaactga tcgacagcta tcagggcacg
    12961 gtattgctgg ttagccacga tcgtcagttt gtcgataaca ccgttacaga atgttggatc
    13021 ttcgaaggcg gcggtaaaat tggtcgttat gtcggcggtt atcatgatgc ccgtggtcag
    13081 caagagcagt atgtggcgct caaacagcct gcggtgaaaa aaaccgaaga agccgccgcg
    13141 gcaaaagcag aaactgtaaa acgcagcagt agcaaactaa gctataaatt gcagcgcgaa
    13201 ctggagcagc taccgcaatt gctcgaagat ctggaggcga agctggaagc cctacagacg
    13261 caggtggcgg atgcttcctt cttcagtcag ccacatgagc agacacaaaa agtgctcgcc
    13321 gatatggctg ctgcagagca ggagctggag caagcctttg aacgctggga gtatcttgaa
    13381 gcgttaaaaa atggtggctg atcgcaaaat agtcgattta aacagagaca gctgacaacg
    13441 gtaaaattgc ctgatgaact gggtttatca ggcatgaaaa ccaacacttt gttagtcatc
    13501 tgtaaaatag cgcatcatta aggagtacca atgtgcgaac atcatcatgc cgcgaagcac
    13561 atcctgtgct cgcagtgtga catgctggtg gcgttaccgc gccttgagca tggtcagaaa
    13621 gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt gggatgcccc tcggcagcgt
    13681 ccgaccgcct atgcgttggc tgcactgttc atgctgttgc tgtccaactt gtttcctttt
    13741 gtgaatatga acgttgcagg agttaccagt gaaattacat tactggaaat tcccggcgtg
    13801 cttttttctg aggactacgc cagcctcggc acctttttcc tgttgtttgt gcaactggtt
    13861 cccgcgtttt gtctgataac cattctgtta ctggtgaatc gcgcggaatt accggtccgt
    13921 ttaaaagagc aactggcacg ggtgcttttt caactcaaaa cctggggaat ggcggagatt
    13981 ttcctcgctg gtgtgctggt cagtttcgtt aaactgatgg cttacggcag cattggggta
    14041 ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc aactgcgcgc ttttcagtgc
    14101 gttgatcgtc gctggttatg ggacgacatc gccccgatgc cagaactgcg ccagccgcta
    14161 aaaccaggcg tcacggggat acgtcagggg ctgcgttcgt gctcctgttg tacggcaatc
    14221 cttcctgctg atgaacccgt gtgcccgcgt tgtagtacca aagggtacgt tcggcgtaga
    14281 aacagcctgc agtggacact cgcgctgctt gtaacgtcca tcatgctgta tcttccggct
    14341 aatattttgc ccatcatggt gacggattta ttaggctcga agatgccgtc gacgattctc
    14401 gctggggtca ttctgttatg gagcgaaggc tcttatcccg tcgctgcggt gatctttctg
    14461 gccagtatta tggtgccaac gttaaagatg atcgccatcg cgtggctgtg ttgggatgcc
    14521 aaagggcatg gcaagcgcga cagtgaaaga atgcatttga tttatgaagt tgttgagttt
    14581 gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg tgctctcggc gctggtgcgt
    14641 atgggaggtt taatgagtat ttatccggca atgggtgcat taatgtttgc tttagtcgtc
    14701 ataatgacaa tgttttctgc tatgacgttt gacccgcgtt tgtcgtggga tcgtcaacct
    14761 gaatcagagc atgaggagtc ctgacagtat ggaatctaat aatggggaag ccaaaatcca
    14821 gaaagtgaag aactggtctc ccgtgtggat atttcctatc gtcacggcgc tcattggggc
    14881 ctgggttctt ttttatcatt acagccatca gggactggaa gtgaccctga tcaccgcgaa
    14941 tgcggaagga attgaaggtg gcaaaaccac cattaaaagc cgtagcgttg acgtcggcgt
    15001 ggttgaaagc gccacactgg ctgatgattt gacgcacgtt gaaatcaaag tgcggctgaa
    15061 ttctggtatg gaaaaattgc tgcataaaga caccgtcttt tgggtggtga aaccgcagat
    15121 tggtcgcgaa gggattagcg gcctgggaac gctgctgtct ggagtttata tcgaactgca
    15181 gccaggcgcg aaaggcagca aaatggataa atacgatttg ctggactcgc caccgttggc
    15241 cccgcctgat gcgaaaggta tccgtgtgat tctcgatagc aaaaaagccg ggcagctctc
    15301 gccaggagat ccggtgctgt tccgtggcta tcgggtaggt tcggttgaaa ccagcacctt
    15361 cgatacgcaa aaacgcaata tcagttatca actgttcatc aatgcacctt atgaccgact
    15421 ggtgaccagc aatgttcgct tctggaaaga tagtggcatt gcggttgatc tgacgtcagc
    15481 ggggatgcgt gtggagatgg gctcattgac aacgctgctt agtggcggtg ttagctttga
    15541 tgtgccggaa ggtctggatt tagggcagcc agtggcaccg aaaacagctt tcgttttgta
    15601 tgatgatcag aagagcattc aggattcgtt gtacaccgat cacattgatt atctgatgtt
    15661 ctttaaagat tcggtacgcg gtctgcaacc gggagctccg gtagaattcc ggggtattcg
    15721 cctgggtacc gtaagcaaag tgccattctt tgcgccgaat atgcgtcaga catttaacga
    15781 tgattaccgt attccggtac tgattcgtat cgagccagag cggctgaaaa tgcagcttgg
    15841 cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg aaacgtggtt tacgcggatc
    15901 gctgaaaacc ggaaacctgg tcactggtgc actgtatgtt gatcttgatt tctatccaaa
    15961 tacgcctgca ataaccggta ttcgtgaatt taatggttat cagattatcc cgactgttag
    16021 cggcggcctg gcgcaaatcc agcaacgact gatggaagcg ttggataaga tcaacaaact
    16081 gccattgaat ccgatgattg aacaggcaac cagtacgctt tctgaaagtc agcgcacaat
    16141 gaaaaacctg caaacgacgc tggatagcat gaacaagatc ctcgccagcc agtcgatgca
    16201 gcagttgccg acggatatgc agtcaacgtt gcgtgaattg aatcgcagca tgcagggctt
    16261 ccagcctggc tccgcagcct acaacaagat ggtggcggat atgcagcgcc ttgatcaggt
    16321 gttgcgagaa ctgcaaccgg tgctgaaaac gctcaatgag aagagtaacg cgctggtatt
    16381 tgaagcgaag gacaaaaaag atccagagcc gaagagggcg aaacaatgaa aaagtggcta
    16441 gtgacgattg cagcactgtg gctggccgga tgcagctccg gcgaaattaa taaaaactat
    16501 taccagttac ctgtggtgca gagcggtaca caaagtaccg ccagccaggg caatcgtctg
    16561 ttatgggtag agcaggtcac tgttcctgac tatctggcgg ggaatggtgt ggtttatcaa
    16621 accagtgatg tgaagtatgt gattgccaac aacaacttgt gggccagccc gttggatcaa
    16681 caattgcgca acaccctggt tgccaacctg agtacgcaac tgcccggctg ggtggttgcc
    16741 tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta ccgtaacgga gtttaacggt
    16801 cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt tgaaccacca gggacaactg
    16861 atcaaacgtc cgttccgtct ggaaggagtg caaactcagg atggttacga tgagatggtt
    16921 aaagtactgg ccagtgtctg gagtcaggaa gccgcttcta ttgcgcaaga gataaagcgt
    16981 ctaccttaat tataaagatt tgtaaatata accgtctccg gtatgttgcc tgaggcggtt
    17041 tttttgtttc taacgtgcgg aaaaagttgt tcctctttac attttttgta caaccgacat
    17101 acccgtgtag ctcacaaata tgacagtggc gtgaattttg cgcattgacg gcagttatga
    17161 ttcgcggtat tgcttaactg tgattgcaca tttagtaatc actgttttct tttccaccag
    17221 aaaccagtat gagggaaacg aggcatgaag agacaaaaac gagatcgcct ggaacgggca
    17281 catcaacgtg gttatcaggc cggcatcgcc ggacgctcaa aagaaatgtg tccctatcag
    17341 acgctgaatc aaaggtcaca atggctggga ggctggcgag aagccatggc ggacagggta
    17401 gtaatggcct gattctgtct ctttaaaaag aaacctccgc attgcggagg tttcgccttt
    17461 tgatactctg tctgattata atcagaaggc agaagtatcc tggaacagac cgactttcag
    17521 gtcgctggcg gtatagatca aacgaccatc aaccagcact tctccatccg ccaggcccat
    17581 aatcagacga cggttaacaa tgcgtttaaa gtgaatacgg taggtcactt ttttcgctgt
    17641 cggcagtacc tgaccagtga atttcacttc gccaacgccc agtgcgcggc ctttaccttc
    17701 gccgcccagc cagccgaggt agaaccctac cagctgccac attgcgtcca ggcccaggca
    17761 tcccggcata accggatcgc caataaagtg gcatccgaag aaccacagat ccggattgat
    17821 atccagttct gcttcaacat accctttgtc gaagttacca cccgtttcgg tcattttgac
    17881 cacacggtcc atcatcagca tattcggtgc tggcaattgc gggcctttag cgccaaacag
    17941 ttcaccgcga ccagaggcaa gaaggtcttc ttttgtatag gattcgcgtt tatctaccat
    18001 gttctctgta agccttattt tattgaagca cgcaggatag ctaacacgtg tacgctgaac
    18061 aagtccgatc agttcggaat aaaccagttc agccaacgta atggccatgg aaaacggtga
    18121 cgtccttcct gttgcgatgc ttgcgcgata cgttcctgga tggtttgcat cagcgtcgtt
    18181 tggccttcgc catcccacac cagatttaat aataacggca gtgcgtcagt cacatcgtct
    18241 actgcccaga tggtgaattt gccttcttct accgctttca ctagttcact gtgaagactt
    18301 aaatggcgaa cgttagctgt ggggataatg acaccttgtt tcccggttaa ctcacgttgc
    18361 tggcaaatag caaagaagcc ttcgattttc tcatttaaac caccgaccgg ctgggcgcga
    18421 ccgaactgat cgactgaacc tgtgatagcg atactctgat tcaccggcac atcggcgagg
    18481 gcgcttatca gggcgcagag ttcagccatc gaggcactat ctccatcaac ttcactgtat
    18541 gactgctcaa atgtcagcga tgctgagaag gggatctgtt gctcaagctg tagttccgac
    18601 atcaggaacg cttgcatgat catcatccct ttcgcatgga tattgccgcc aagctccgct
    18661 ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca caacgcagct aatgcgagaa
    18721 ggttcgccaa aagcgcgtgg atgacccgga aattcaatga ccgaaagggc gttaatttgc
    18781 ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct gaaggatctc atcctgcata
    18841 cgttcagcga ggaaaccttc gcgccattca cgctgctgca gcattaagtt tagctgctcg
    18901 ccggagaagg tgtcgccatc acacagggag gcgacctctt tacactggcg gaggatccac
    18961 tgcgggctaa gcggaagtgt ttcttgttca ccggtgtagc gtgctgcttc gcggataagt
    19021 accggccagg catccgctcc cggtgcaggt aagtgattat gtctggcggt aaatgtcacc
    19081 cagcgacacc actgggttac tgactccgca tcgacaatct gcagagtatc ttcaaattcg
    19141 ctataaatag cctgctctga aagctctggc tccatctcct ggaaatcagc caatgattcg
    19201 cgttcgccta ccagaatgac tttcagcttc aatggcatcg aaggcacaga gacggggaga
    19261 gggcgcgact catcaaacgc aacccagtca aaacgctcgc ggttaacgat atttttcagc
    19321 cgcatccaca gcagaggttg cgccagcagt gtacgcaaag agatgatgag aataccgcca
    19381 tttgcctgat gcaccagacc aggctgcagg gtaatgtcgc cattaaactg gcgcaggcag
    19441 ccaaagagtt gctccgcttc tacccagtcg gcagcgacaa cttgcgttaa agtcgcaaaa
    19501 ttatcatctg cactcactgc gtgacgtaag cggatggagt ggccggaaac ctcatagtga
    19561 ccgcccacca gttggcctgc atcgctttgt agcgtacgcg cggcattggc aataagattc
    19621 agatactcag actcttccgg ggccttcgcc agcataaaag aggaggatgc tcgcgtatgc
    19681 agcaactgct ccagcgcaaa ttgcagccgc ggttgagtat cactgaataa aggatcgttt
    19741 tcgtcaatca aatgtggctg agcaaatatt tcctgatagc tatcggtatc aggaaccagg
    19801 tcacgccatg caagtttcgt aatggtcaaa gttgatgttt tttagtctgt tgtcaaagcc
    19861 gcgattatac cgtaaccggc actacagcac acgtagaaag caccgacaat actccaggca
    19921 tgggcgttaa agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt
    19981 ctgtaaagag ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct
    20041 tgaaagcggt tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaatcac
    20101 ccgttacata gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctggaaaa
    20161 tgaacccgtg ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg
    20221 catgaagcaa accattcggg caagacgaaa gcgccatttt aatgcggaac accagcatac
    20281 gcgcaaaaaa tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcgca
    20341 gcgtcgcggt aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa
    20401 agagaaatac gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa
    20461 ggaataactg acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg
    20521 ggtttttcta ccagacgaga acttaagcct gcggctgagt tacaacgtct ttgatacctt
    20581 taacttcgat ctctacgcga cgatccggag ccaggcagtc gatcagtgca gcacgctgtt
    20641 tcacgttgtc acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga
    20701 tcttgtctgc cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct
    20761 cggacagacc ctggttgtaa gcgtcagaac cgatgcggtc ggtgtaaccc agaacaacta
    20821 cggaaccgtc tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct
    20881 gaccttccgg tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga
    20941 agtgcttggt ctgtacttcc ggtgccggag ctggagccgg agcaactact ggagctgctt
    21001 cgccctgacc gaaacggtag gaaaccccca ggctcagcat gccgttgtcc ggacgagtgc
    21061 cgatggtgtg tgcgtcaccg atgttgttgg tccactggta ttccagacgg gtagcgattt
    21121 caggagtgat cgcgtactca acaccgccag cgaagaccgg agaaacgccg gtgtcgtggt
    21181 ttttaccata aacgttggat ttagtgtctg cacgccatac cataccaccc agacgagtgt
    21241 agatgtccag gtcgtcagtg attgggtaac ccagtttagc ggtcagttga acgccctgag
    21301 ctttgtatgc accgttttca acgctgcctt tgtacggcat acgacctaac cagtcgtaac
    21361 ccatttcaaa gccaacatac gggttaacct ggtaaccacc aaaagcacca gcgcccagtt
    21421 ggttttcatg ggtcgggcca ttgttgttga tgaaaccagt gtcatggtac tgggaccagc
    21481 ccagtttagc accagtgtac caggtgttat ctttcggagc ggcctgcgct acggtagcga
    21541 aaccagccag tgccactgca atcgcgatag ctgtcttttt cattttttgc gcctcgttat
    21601 catccaaaat acgccatgaa tatctccaac gagataacac ggttaaatcc ttcaccgggg
    21661 gatctgctca attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21721 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21781 gtcttgtata aggtatgttt aatctttttt gtcagcgaca atttacagaa gaaaatcgcg
    21841 gaaaccgctt cagacaagcc tccgcaagga aaattagtca cgactgaaag cattggctgg
    21901 gcgacaaaaa aagttccagg attaatccta aatttactta atgatacaaa ttagagtgaa
    21961 tttttagccc ggaaagttgt ctcgtggcgt gagaggatgc gcttaccgga cgcataataa
    22021 accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
    22081 aatcatctgc caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
    22141 ttgactccac agtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta
    22201 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
    22261 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
    22321 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
    22381 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
    22441 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtacagtac atccagtgac
    22501 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa
    22561 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg
    22621 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
    22681 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
    22741 tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat
    22801 ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct
    22861 gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat
    22921 gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca
    22981 gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg
    23041 tcttttcatc tggaagcgat tctcggggag gtggggatta aggatgtacg ggcgttacgt
    23101 atacttgggg caaaaatgtg ttggttgcga ctgcggcagc aaaacagtct ggtgacagaa
    23161 aagattctgt ttatgcttga aggtgccatt atcggcattc atgaagctgc gctcccggtg
    23221 gcacgccgcc aggagcttgc agaatgggct gactctctta cgccgaaaca ggagtttcct
    23281 gcggaacttg agtaatctgg cgttgcagac gaccaatctc aggcagtaat gcaatcaata
    23341 atccaacttg ttgtacgacc agaggttctt tgctgtctgc ccgtggttca agttgctgca
    23401 tccgctgttt caggctagct aatgcctcat tgacgcgttc ttcatcagca ggttgatgat
    23461 gtaacgcgtc atcaacatag caaactgcgt catcaagaaa cgccagaatt tcaggattag
    23521 ttaactgctc ccggtgagca ccgagggctg agatatagct ggtaaacgta tggttaaggc
    23581 acagcaaccg aaacgcggct tcgcgaattt gcggggtaac gttcggctcg ctggacatat
    23641 ttgataccac cgacgccagc tcagcatcac ggttgtgtgc atcgcggcgg gcaatacgat
    23701 acgccagacg gttatcacgc ccctgatggt attgctccag tatggcatcg agataccgac
    23761 agttggcctc tgtggcgcgt tcgagcatgc gcggcagatt gcgaaactgc cagtcaggcc
    23821 agatgtagct cactgccgcc cacgcaatgg cacaaccaat cagcgtatcg attacgcgag
    23881 gtaacgctac ttcaaaacct tcacccagta agttaaaaca cagtagcacc aaaagtgtga
    23941 tgaacatcgt tgcatgagcg tattgcacgt tacggaaggc aaaaaagagc acgccggtaa
    24001 taaccagcag caccagctgc ccttccagtg atggcacaaa ccacagcaca ggaatgccaa
    24061 tggcgatacc taccagcgta ccaataatcc ttaacttcag gcggtggcgc gtggcgttat
    24121 agtttggctg gcagacaaac aaacttgtca gcaagatcca atacccgtga tgcattccgg
    24181 ttatctgaat gatggcgtag ccgaagcaca acaccagcga cattcttacc gcatgacgga
    24241 agagggcgga ttccggcgtg aagtgacggc taagacgcag ccagatatca ctcaacccgt
    24301 gcgggctgtc atcagcgagc tcattttcgt cattattatg gggtagtgcc tgggcctgtt
    24361 ctgattcaat tgttgccagt tgggcatcaa tggcgcgtaa attgttcagc aaaaatccca
    24421 gtgttttgag taaatcggcg ggtgcgccgt tatcgcgcat ccgctccagc gcagcatcaa
    24481 tatgcgtaaa agcgcgctca aaatgcggat catgttgata aggctgacgc aacaaaatac
    24541 agcgtgacag ttgctggcac gcctggccct gcatcgacat cagccgctga aaacggaaca
    24601 gcacgtcgct gtggcgaaaa tgttcacgca atgtttgata ctgaatatga gaagagctgg
    24661 cacgctcgtg aatatcctgt gcgacaaagt aataatgcag cgtgcgacgc gttccccgtt
    24721 gaccacgatc gccacgtaag cgggtcagca gcgagagttt cgtctgattc aatgtcgcca
    24781 tcagcagacc gttggcgaga gccaaatcgt acagcggtgc ctggctttca tcttcaatat
    24841 caggatcaaa catgcgcgac ttgagctcaa gataacgcgc cagttgttca tagcaacgcg
    24901 ccaggttgtc ctgcagcggg cggaccggga acagcagatg accaataagt gtcaggacgt
    24961 tgtaccagac ggcaccggcc agcagataca tcggctgctg ataccagtgc tcatacagtg
    25021 atgttcccaa catagtgtaa atggcgatca gcaatgcacc gaaggcaatt gttgcatagc
    25081 gttgacccag accgccgagc aaaatgaagc cgctggtaga gagcgttaag ccaatcgcaa
    25141 atagccaggg ccagggaaac agcaattcta ctgaggccga ggcgataaaa aagcagaaca
    25201 gcgtaatgat gaggttacgc aaacgtcccg ccagtcggtc atcgagatcg gtcagcgccg
    25261 ctgccaccat ccccagcgtt agcggaatcg tcagttttac atcacccagc caccacggaa
    25321 acgctgtggt tccacaaagc gcaataaaaa tacgcgcgta atacagccag gcgctgttcc
    25381 aggtatagcg tttgagcaaa ggacttagca taaaggccat attagtggat tattcaaaac
    25441 gacgacgtgc attggcttca cgcgcagctt gcgctgtttc taccgatacc acgcgacgac
    25501 caaccggcca tagtgcaata gcggcaattt taaagttcgc aatgccgaca ggaatgccaa
    25561 tgattgaaat acattgtgcg atgcccgttg caatgtgcat caggcataac caccagccaa
    25621 agaaaatcag ccagaaaata ttcaataccg taccgccagt attcagcagc acatttttgc
    25681 cagccgggtt cagttcatcg acatgaatag cttcattgcc ataaggcacc agagatagtt
    25741 tagtgatctc ccagcaggat cgtgtcagcg gtaaggtaaa aatcagcaca atactgacca
    25801 gagtcgccaa cagccagccc agagtggtgg caaatccgcc aagcacaaag ttcagaatgt
    25861 tcaaaacggt acgcataaaa cctcgcttta ctgtggtttt cagtaatggg cggcaattgt
    25921 aacgtttttt tgggctggag cacgttttct ctgacggtta cactgataag aaataatttc
    25981 gtgtggatct acagagtcat ggaactgaaa gcgacaacgc ttggaaaacg tctggcacag
    26041 cacccttacg atcgggcggt gatcctcaat gccgggatta aagtctccgg cgatcgccac
    26101 gaatacctta ttcctttcaa tcaattactg gcgattcact gtaagcgcgg tctggtatgg
    26161 ggcgagctgg aatttgtact gccggacgaa aaagtggtgc gtctgcacgg caccgaatgg
    26221 ggcgagacgc agcgttttta ccatcatctt gatgctcact ggcggcggtg gagtggcgag
    26281 atgagcgaaa ttgcgtctgg tgttttacgc cagcaactgg atttgattgc cacgcgcact
    26341 ggagaaaata aatggctgac gcgtgagcaa acctctggcg ttcagcaaca aatccgccag
    26401 gctttgtcgg cgttgccgtt gccggttaac cgactggaag aattcgataa ctgccgtgag
    26461 gcgtggcgta aatgtcaggc ctggttgaaa gatattgaaa gcgctcggtt gcagcataac
    26521 caggcgtata ccgaagccat gcttaccgag tatgcggatt ttttccgcca ggtcgagtct
    26581 tcaccgctga atccggcgca ggcccgggca gtcgttaatg gcgagcattc tctgttagtg
    26641 ctggcaggtg caggaagcgg aaaaacgtcg gtgctggtgg cccgtgcagg ctggttgctg
    26701 gcgcgtggtg aagcgtcccc tgagcaaatt ttattgctgg cgtttggtcg caaagccgct
    26761 gaagagatgg acgagcggat tcgcgaacgg ctacataccg aagacattac cgcacgcacg
    26821 tttcatgcgc tggcgctgca tattattcag cagggcagca aaaaagttcc gatagtcagc
    26881 aaactggaaa atgataccgc tgcccgtcat gaactcttta ttgctgagtg gcgcaagcaa
    26941 tgcagcgaaa agaaagcgca ggcgaagggc tggcggcaat ggctgacgga agaaatgcag
    27001 tggtcagtgc cagaaggtaa cttctgggat gatgaaaaat tacagcgtcg ccttgcctct
    27061 cgcctcgatc gctgggtaag tctgatgcgg atgcacggtg gtgcacaggc agaaatgatt
    27121 gccagtgcac ccgaagagat tcgcgatctg ttcagtaaac gtatcaagtt gatggccccg
    27181 ttattaaaag cctggaaagg tgcgctgaag gcagaaaacg ctgtcgattt ttcgggcctt
    27241 attcatcagg cgattgtgat tctggagaaa ggtcgcttta tcagcccgtg gaagcatatt
    27301 ctggttgatg aatttcagga tatctcgccg cagcgggcag cgttgttagc ggcattacgc
    27361 aagcaaaaca gtcagacgac gttgttcgct gttggtgatg actggcaggc gatttaccga
    27421 ttcagcggtg cgcaaatgtc gctcaccacc gctttccatg aaaactttgg tgaaggcgaa
    27481 cgttgtgatt tagacacgac ttaccgtttt aacagtcgta tcggtaaggt ggcaaaccgg
    27541 tttattcagc agaacccagg ccagctgaaa aagccgctaa acagcttaac caatggagac
    27601 aaaaaagccg tcacgttatt ggatgagagt caacttgacg ctttgctgga taagctctct
    27661 ggttatgcca aaccggaaga gcgcattctg atcctggcgc gttaccatca catgaggcct
    27721 gccagcctgg aaaaagcggc aacacgctgg ccgaagttgc aaatcgactt tatgaccatt
    27781 catgccagca aagggcaaca ggcggattac gtcatcatcg ttggcttgca ggagggaagt
    27841 gatggttttc cggctgcggc gcgggagtcg attatggaag aggcgctact gccaccggtt
    27901 gaggatttcc cggacgctga agaacggcgg ttaatgtacg tggcgctgac ccgggcacgc
    27961 catcgggtat gggcactgtt taacaaagag aatccctctc cctttgtgga aatactgaaa
    28021 aatctggatg tgccggtggc gagaaaaccg taagaaacag gtggcgtttg ccacctgtgc
    28081 aatattactt cagacggtcc gcgagataac gctgataatc ggggattaga atatcgaccg
    28141 cgtcgttgaa atgcggcgac tggattatga agtctgccgt tgccacgttg gtggcgaccg
    28201 gaatgttcca taccgtcgcc agacgcagca aggctttcac gtcaggatcg tgcggcacgg
    28261 catttagtgg atcccagaag aaaatcaata catcaatttt cccttctgag atcaatgcgc
    28321 caacctgctg gtcacccccc attgggccac tcaacatcgc gttgacgttc atgccggtcg
    28381 cgcgggaaat taagttaccg gtagtgcctg ttgcatacag tacgtgttgt tccagtaacg
    28441 gttgatgccg ttccacccag ctcatcagca tttgtttgca gtgatcgtgt gccaccagcg
    28501 caatatgttt ccgcgcaggt aaagtgcgag tcgtcagttc cataatgtac atccgtagtt
    28561 aactttccta cagattactg taagcactta tcgctgcaag ataaagaccg aaaaagcctg
    28621 cgcacaggca caaaaatctc aggaagatgg ttgtttttcc gcccactgca ggaaagtatt
    28681 tcgcgtttgt gggtcagcca gtttaaacca atacttcagc cgttgttctg tgagcacctg
    28741 agactgcggt ggaatagcat ccagctcgga aacacccgaa agcagcgtac tgtcatctgc
    28801 catcatcgtg gcaaattgcg gtagtgaagc gcgtttcgcg gatttgttat agcgttcaac
    28861 ctcgacctca taatcaatcg tttttgccgt tgaggtaatg gcgagaatat ccagctttac
    28921 cggaatcggc gtcgcatcgc catccaacaa ttcaaggcgc ggcgcggcat caaaatggtt
    28981 agcttcccgc tcattctcca ggcgaggcag gcgaaaattg acctggttga tgagctgggt
    29041 attaaaactg accaccagcg gtggggagat atacagccgt tcttcactat tggaaagatg
    29101 aatcgtcttc tcaacgcgaa acactaactg atgcggtcca ttatccagtt caatgctgtc
    29161 cgcaccgcgc agcagagagc tggaaacctt cttgccgtcg agtaccagaa gatcgacatc
    29221 ggttgaaagc cgcagcgtgg tggcaaaaac ggataccggc aaacataatg caattaaggt
    29281 ggtcacgatg ccggttttca taggtagctc ctgtcaaaag accgcccgaa taataatgtg
    29341 tcaaaatttt tctttaatca tacttactaa catatagaca tatttcccgc ttttgctctc
    29401 attcattcgt attagctgca tggttggcat gtcggctttg tcgtacactt tgttaaacag
    29461 tctggaggaa ataatgaaag aaaccgatat tgcaggcatt ttaacgtcca ctcacaccat
    29521 tgcgctggtg ggggcaagcg acaaacccga tcgtcccagc tatcgcgtga tgaaatatct
    29581 actcgaccag gggtatcacg tcattccggt ttcgccaaaa gttgccggca aaacgctgct
    29641 gggccagcag gggtatggca cgctggcaga cgttccggaa aaggtcgata tggtcgatgt
    29701 tttccgtaat tcagaagcgg cgtggggcgt ggcacaggaa gctattgcca ttggcgcgaa
    29761 aaccctctgg atgcaactgg gggtgattaa tgagcaagcg gcagtactgg cacgggatgc
    29821 cgggttgaat gtggtgatgg atcgctgccc ggctattgag atccctcgcc tgggcctggc
    29881 caaataaaaa atccccggaa ggcaaaaacc ttccggggat tcgttcaggg atcagttacg
    29941 cagacgcggg gcctggagtt gtttgcggat ggtctgcgcc agctcatcca tggatggctg
    30001 ttcaggatgt tcatcctgca attcgctact tagctgggct tcagccaggt aggtatgaac
    30061 cggtaggccg ttatcgtcct ccatcactac gtgataccac ggagcggcgc gaagctcgtc
    30121 atttaccgcc agctcatcag gcgacggttc agagagcgaa taaaccgggt cgatatccac
    30181 gaccactccg agataaccta acagggaatg gcggacctgc tggccgatac cgaatttgct
    30241 ggcaatcata gtcacctccc gggaaatctt tacctttgat ataagggtaa aattccacat
    30301 ttcaagttac atgacgcgac aggcaaaccc tttcagatat agcccttccg gataggtagc
    30361 gatcaccgga tgatcggctg cctgacggaa ctgctctata aattgtacat cacggccggc
    30421 atcaattgcg gcatccgcga tgattttctg aaataaatcg ctggtcatca gaccggaaca
    30481 ggagaaagtc aggagaatac cgccttcatt cagcaactga atcgccagca tgttgatatc
    30541 tttatagcca cgacacgcgc ccatcaactg gcttttattc tcaacaaact tcggcgggtc
    30601 catcacgata acgtcaaatt tttcaccgcg atcgcgataa gtacgcagca atttaaagac
    30661 atcatcacgg acaaactcag ccttgctcag atccagtttg ttcagctcaa cgttctgccg
    30721 tgcaatatcc agcgcttcct aggaggtatc aacgctgaca acctggctgc aaccgcccat
    30781 cagtgccgat acggcgaaac caccggtata ggagaaacag ttcagcacac gtttattttc
    30841 aacgtagcgg cgggtagcca ggcggctgtc tcgctggtcc aggtagtagc ccgttttgtg
    30901 tccgtgctga atatccacca gcagcttcat gccgtgttct tcaatcggca gcagggcagg
    30961 cggcaactcg ccggtgacga ggccctgggt cagctccatc ccttcttttt tacgtaccgc
    31021 aacatcgctg cgatcgtaaa tcgcacattc cgggtacagc gtttgcaggg cactaattaa
    31081 tgccgcgcgc tgatattctg ccccagcact cagcagttgc agcaccagaa aattaccgaa
    31141 acgatcgata gtaatacccg gcaggccatc agattctccg gcgattaaac gatagctgtc
    31201 gaggccatct ttttgcgcca gtcagtcacg ccatttttgt gcttgttgca aacggcggga
    31261 aaaaaaagca atgtcgatag actcagacgg gtcaaacgtc cagacgcgcg cccggatttg
    31321 cgaagctggc gaataagcgc cgcgtgctaa ccattttccc tgatgatcaa caatatcgat
    31381 ggtttcaccg aggctggctt taccttccat gcgggcaacg gccccggaaa agacccacgg
    31441 atggcgacga agtaatgatt tttcgcgccc tttggctaac actaaacgta cactcataat
    31501 ttacttttct gtcgatgcaa agaaatgggc gtcattgtcc ggagttttcc atggatttgc
    31561 aacgcgtcca ttaaggataa ggagagcagc aatgtcgaaa gtctgcataa ttgcctgggt
    31621 ttacgggcga gttcagggcg taggatttcg ctacaccaca cagtacgaag cgaaaagact
    31681 gggtttaacc gggtacgcca aaaatcttga cgatggcagc gtggaagtgg ttgcctgcgg
    31741 tgaagaaggg caggtggaaa aattaatgca gtggttaaag agtggcggcc cgcgttctgc
    31801 gcgtgtagaa cgggtgctca gcgagccgca tcatccctcg ggggaattaa ccgattttcg
    31861 tatccgttaa atacatttta ccggttttgg caggccagca attttggtgg cttgcttagc
    31921 cggacctttc ggaaacagtc ggtataaata gcggctgtta cctttttctt cgccaaattt
    31981 attcgccatc gcttttacca gcatacgaat cgccggagaa gtattgaatt ccagatagaa
    32041 atcacgcaca aaacgcacca cttcccagtg ttccggtgac agcgaaatcc cttcgttctc
    32101 tgcaatcacc actgccagcg gctcactcca ctggctgctt tctttgagat agccttcggt
    32161 atccgtttct atctctttac cttcgaagat cagcataatt attactacct taatcagact
    32221 ggcggcagtg taacaaaaaa caaagccccg cataaagcga ggctatgaaa gtgttagcgg
    32281 gtgagattaa tcgcggctgg cgaagcccag aatgctcagc aggctgacga agatgttgta
    32341 cagcgaaaca tacaggctaa ccgtggcacg aatatagttc gtttcaccgc catgaacgat
    32401 gttgctggtt tcaaacaaaa tagcgccaga ggagatcaga atgaagaccg cgctgatcgc
    32461 cagatgcaga gcaggcagct gcaggaagat attagcaacc ataccaatca gcaccaccac
    32521 aatacccgcc atcagcatac cgccgaggaa cgacatatct ttgcgggtgg tcagcacata
    32581 tgcagagcag cagaagaaca ctaacgccgt tccgcccagt gccatagcga ttacgtcacc
    32641 cattccggca gacagatagg tgttcagaat aggtccgagg atataaccca gaaaaccggt
    32701 aaaggcgaat gcggagataa tcccggtcgg cttattcgcc gttttatagg tcaggaacat
    32761 caaaccatac atacccacca gcgtcagaat cagacccgga gatggcagca tcagcaccgt
    32821 actggcagtt gcggtaatcg ccgaaaaggc cagcgtcagg ctcagcagaa aataggtatt
    32881 acgcagcacc ttatgggtgc taagcagtga tgtacggtca tgtgaagaac taacaatacg
    32941 atccattgag tcactctcta tgacagatgt aattaattaa gcagcataat gataatgcgt
    33001 aagggcaccc agaagtttta cccatcttta cgcatttgat ctggaacagg tttaacagcg
    33061 gattatcagg tcattaagca aatataacgc cctgagaatt tcgacaggca aaagaaaaag
    33121 gggttagcat ttagctaacc ccttatctta tttggcggaa gcgcagagat tcgaactctg
    33181 gaaccctttc gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg ccacacttcc
    33241 ggaatgacgc gcactataaa catcccgatg cggcgtgtaa acccctaatt tgtttgtttg
    33301 cctgaaaaac agccaaaagt gcattgatag cgtgaaataa cagcagattg atcatttcat
    33361 caccatgaat tccttctctt ttactcgttt agcaaccggc taaacatccc caccgcccgg
    33421 caaaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag tttgtattgt
    33481 tttgtgcaa
//
LOCUS       DAZPLM010000020        33375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPLM010000020 DAZPLM010000000
VERSION     DAZPLM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.020"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(128..268)
                     /gene="yjjY"
                     /locus_tag="ACHVC7_000834"
     CDS             complement(128..268)
                     /gene="yjjY"
                     /locus_tag="ACHVC7_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR2114952.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            364..1080
                     /gene="arcA"
                     /locus_tag="ACHVC7_000835"
     CDS             364..1080
                     /gene="arcA"
                     /locus_tag="ACHVC7_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR2114953.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1140..2492)
                     /gene="creD"
                     /locus_tag="ACHVC7_000836"
     CDS             complement(1140..2492)
                     /gene="creD"
                     /locus_tag="ACHVC7_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR2114954.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2550..3974)
                     /gene="creC"
                     /locus_tag="ACHVC7_000837"
     CDS             complement(2550..3974)
                     /gene="creC"
                     /locus_tag="ACHVC7_000837"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR2114955.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3974..4663)
                     /gene="creB"
                     /locus_tag="ACHVC7_000838"
     CDS             complement(3974..4663)
                     /gene="creB"
                     /locus_tag="ACHVC7_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR2114956.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4676..5149)
                     /gene="creA"
                     /locus_tag="ACHVC7_000839"
     CDS             complement(4676..5149)
                     /gene="creA"
                     /locus_tag="ACHVC7_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR2114957.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5360..6229
                     /gene="robA"
                     /locus_tag="ACHVC7_000840"
     CDS             5360..6229
                     /gene="robA"
                     /locus_tag="ACHVC7_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR2114958.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6226..6873)
                     /gene="gpmB"
                     /locus_tag="ACHVC7_000841"
     CDS             complement(6226..6873)
                     /gene="gpmB"
                     /locus_tag="ACHVC7_000841"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR2114959.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6925..7446
                     /gene="yjjX"
                     /locus_tag="ACHVC7_000842"
     CDS             6925..7446
                     /gene="yjjX"
                     /locus_tag="ACHVC7_000842"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR2114960.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="ACHVC7_000843"
     CDS             complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="ACHVC7_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR2114961.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="ACHVC7_000844"
     CDS             complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="ACHVC7_000844"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR2114962.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10095..11762
                     /gene="ettA"
                     /locus_tag="ACHVC7_000845"
     CDS             10095..11762
                     /gene="ettA"
                     /locus_tag="ACHVC7_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR2114963.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11923..12030)
                     /gene="nadS"
                     /locus_tag="ACHVC7_000846"
                     /pseudo
     CDS             complement(11923..12030)
                     /gene="nadS"
                     /locus_tag="ACHVC7_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(12069..13301)
                     /gene="nadR"
                     /locus_tag="ACHVC7_000847"
     CDS             complement(12069..13301)
                     /gene="nadR"
                     /locus_tag="ACHVC7_000847"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR2114964.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13322..14704)
                     /gene="radA"
                     /locus_tag="ACHVC7_000848"
     CDS             complement(13322..14704)
                     /gene="radA"
                     /locus_tag="ACHVC7_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR2114965.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14753..15721)
                     /gene="serB"
                     /locus_tag="ACHVC7_000849"
     CDS             complement(14753..15721)
                     /gene="serB"
                     /locus_tag="ACHVC7_000849"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR2114966.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15827..16471
                     /locus_tag="ACHVC7_000850"
     CDS             15827..16471
                     /locus_tag="ACHVC7_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR2114967.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16499..17515
                     /gene="lplA"
                     /locus_tag="ACHVC7_000851"
     CDS             16499..17515
                     /gene="lplA"
                     /locus_tag="ACHVC7_000851"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR2114968.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17547..17810
                     /locus_tag="ACHVC7_000852"
                     /pseudo
     CDS             17547..17810
                     /locus_tag="ACHVC7_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17971..18690)
                     /gene="deoD"
                     /locus_tag="ACHVC7_000853"
     CDS             complement(17971..18690)
                     /gene="deoD"
                     /locus_tag="ACHVC7_000853"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR2114969.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18770..19993)
                     /gene="deoB"
                     /locus_tag="ACHVC7_000854"
     CDS             complement(18770..19993)
                     /gene="deoB"
                     /locus_tag="ACHVC7_000854"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR2114970.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20045..21367)
                     /gene="deoA"
                     /locus_tag="ACHVC7_000855"
     CDS             complement(20045..21367)
                     /gene="deoA"
                     /locus_tag="ACHVC7_000855"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR2114971.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21494..22273)
                     /gene="deoC"
                     /locus_tag="ACHVC7_000856"
     CDS             complement(21494..22273)
                     /gene="deoC"
                     /locus_tag="ACHVC7_000856"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR2114972.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22531..24081
                     /locus_tag="ACHVC7_000857"
     CDS             22531..24081
                     /locus_tag="ACHVC7_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR2114973.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24053..24916
                     /locus_tag="ACHVC7_000858"
     CDS             24053..24916
                     /locus_tag="ACHVC7_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR2114974.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25432..26214)
                     /locus_tag="ACHVC7_000859"
     CDS             complement(25432..26214)
                     /locus_tag="ACHVC7_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2114975.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26211..27284)
                     /locus_tag="ACHVC7_000860"
     CDS             complement(26211..27284)
                     /locus_tag="ACHVC7_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR2114976.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27406..27567)
                     /locus_tag="ACHVC7_000861"
     CDS             complement(27406..27567)
                     /locus_tag="ACHVC7_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR2114977.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27694..28299)
                     /gene="osmY"
                     /locus_tag="ACHVC7_000862"
     CDS             complement(27694..28299)
                     /gene="osmY"
                     /locus_tag="ACHVC7_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR2114978.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28692..30281)
                     /gene="prfC"
                     /locus_tag="ACHVC7_000863"
     CDS             complement(28692..30281)
                     /gene="prfC"
                     /locus_tag="ACHVC7_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR2114979.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30372..31049)
                     /gene="yjjG"
                     /locus_tag="ACHVC7_000864"
     CDS             complement(30372..31049)
                     /gene="yjjG"
                     /locus_tag="ACHVC7_000864"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR2114980.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31064..31510)
                     /gene="rimI"
                     /locus_tag="ACHVC7_000865"
     CDS             complement(31064..31510)
                     /gene="rimI"
                     /locus_tag="ACHVC7_000865"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR2114981.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31479..31892)
                     /gene="holD"
                     /locus_tag="ACHVC7_000866"
     CDS             complement(31479..31892)
                     /gene="holD"
                     /locus_tag="ACHVC7_000866"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR2114982.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31995..33026
                     /gene="rsmC"
                     /locus_tag="ACHVC7_000867"
     CDS             31995..33026
                     /gene="rsmC"
                     /locus_tag="ACHVC7_000867"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR2114983.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7854 a         9180 c         8545 g         7796 t
ORIGIN      
        1 taaaataatg tgacgaaagc tagcatttag atacgatgat ttcatcaaac tgttaacgtg
       61 ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct
      121 tagcctgtta tgttgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt
      181 cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat
      241 caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca
      301 tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca
      361 aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg
      421 aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg
      481 catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt
      541 aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc
      601 ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac
      661 tacatcacca aaccgttcaa cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc
      721 cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc
      781 aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac
      841 aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt
      901 cagtcccgtg ctgaactgct gaagaaaatg accggccgtg agctgaaacc acacgaccgt
      961 actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg
     1021 gaaatcatcg ccaccatcca cggtgaaggt tatcgcttct gtggtgatct ggaagattaa
     1081 tcggctttac caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacct
     1141 tatttccaga tacgtaattc atcgtccgtt gtaacttctt tactggcttt cattttcggc
     1201 agtgaaaacg cataccagtc gatattacga gtcacaaaca tcatgccggc cagcgccacc
     1261 accagcacac tggttcccaa caacagcgcg ctatcgacag agttgagcag tccccacatc
     1321 actccatcca gcaacaacag cgcgagggta aacaacatgc tattgcgcca gcctttcaat
     1381 accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc
     1441 acggtaaaac cgatatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa
     1501 agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca
     1561 aagaacgcca taaaagtcag tgcaatcagc agaatggcgt acttagtcgc ccggtccgtt
     1621 aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcgaggaa gttttcccag
     1681 ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca gctgctttgc
     1741 cattgcgcct gaaaacctga ctcgctaact tcccgtttgg ctggcaaaaa atcacctaaa
     1801 aaactgggat gcggccagtt gctggttaag gtcatttcgc tattacgccc ggcaggcacc
     1861 acagaaagat cgccggtacc gcttaaattc agggccatat tcagcttcag gttctgcttc
     1921 cgccagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggtg
     1981 ccgggttcaa tggtcagcgc cgttccgtta acttcaggcg ctttcaccac accaatacca
     2041 cgcgcatccc cgacgctaat cacaataaat ggcttgccaa aggtgatatt tggcgcgtcg
     2101 agttcgctaa gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc
     2161 tgaccggtat aaatccctat cttgcgttct tccacgttct gattgccatc aaccatcaat
     2221 gactcaggta accaaaaatg gataaaactt cgtttccgct ccacggtttt atcctcttcc
     2281 tgtaccgtat aaagctcggt cacaggaata gcgataagcg gcccaacaag tttttgcggc
     2341 ccgctggtac tttggcgaat cgcatcttcc acatcgctac ggtaatcggc acgttcgaca
     2401 atcacctgcc gaatcagcat tatcggaatc aacaacagca atactgcacc aaacagggtg
     2461 gtcattttcc agaacaggga ggatttcaac atagtcttct cctttgcaat ggcagcagga
     2521 tacgaaggct atgtgggaag aatttgaagc tatgtgaagt gacggtgaag tcgaagcgag
     2581 gccagcacgc caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg
     2641 acctcactga cgaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa
     2701 gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag gaataccact accggtatcc
     2761 agcaccttaa gcgtgacgtg ttcctgatcc acttcggcgc ttagcgttat acgaccgctc
     2821 tcgggggtaa aatcgatggc gttatccagt aaattcccca gcgcctgatc cagtaacgcc
     2881 ggttcagcag caacattaac ctcggtgggc gtaacatgta gagtgatgtt tttttctgcc
     2941 aactgcacgg tgcgcgcttc gctgacgcgg cggaataatg ccgccacatc aacagcagtc
     3001 aaaacgactt cctgacgatt ctccagtctt gcctggcgta gtaacgtttc caccagtgcc
     3061 tgcattcgcg cattttgcgt cagaatgttg tcagtaaaac gagccactac ttcaggcggc
     3121 ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca
     3181 tgggttaacg cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata
     3241 ctttccagcg cctgcgcgag tttacgcagc tcgctactac cgagatcggg aagaggaacg
     3301 ggcttattgt cagtgacgga atcagcatag cgagtgagcc gggcaatgga gcggttgatc
     3361 caccaaacca tgcctgcgcc aatcaccagt gcgatcccca acaaaatggc gctggcccat
     3421 aaaattcgcc gctcgctgcg tttaatgacc ggagccatcg ccgcgttcgg tttgcctacg
     3481 ctcaaaacgc caataagccg cgagccgtcc ataatcggcg cggcaacata catcaccgaa
     3541 ctttcgggat cggcaggatt ttgcaacgtg ctacgcgcac catactgacc acgcaacgtt
     3601 agccagacgt cattccagcg cgaataatcc tgcccaacgg ctttatttgc cgaatcgaac
     3661 aatactttgc cctgcgcatc ggtcatatag acatgatatt cattgcgcac tttgttaatg
     3721 ccaccgatat tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc
     3781 ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg
     3841 gtatcgatta acgtcccctc cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa
     3901 atagccagta cgaaccaggc cgccaccgcc accagtaaaa aatagcccag cagcaaccgc
     3961 atgccgatac gcattacagg cccctcaggc tatatcccat gccgcgatga gtattaatcg
     4021 gagaaagatc ggggttgatg gcgcgcagct tggcacgcaa cgttttaatg tgggtatcga
     4081 cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg
     4141 accagacgcg gcctggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca
     4201 gcgttaatgg cgtgtcaaac cagctgatct gcgccgcggg ttcattcaat tcaaaatgac
     4261 caatacggat gacgggagac ggcgacgaga acttcttcac ccgacgcagt aaggtgcgca
     4321 ccctggcgca cacttcgcgg ggtgaaaacg gtttagccac atagtcgtca gcgccaattt
     4381 ccagcccaag caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg
     4441 ccggatggag cgccagtaac tggcggcaca attcaaagcc gctgatatca ggcaaaccaa
     4501 catcgagaat catgacgtcg ggaacctgct ggcgagcttt atccagcacc ggcaagcctc
     4561 gctcaaagac ctcgacggca aaaccttcct gctgcaacat gtagaccagc gtgtcggcta
     4621 tcccttgctc atcttccact aaccagaccg tttcccgttg cataaatcgc ctctgttatt
     4681 gccgccacgg catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt
     4741 tgtcggagta agccagataa gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct
     4801 gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc
     4861 cgtttttaat acgatccgac agttcaatcg gcccgacttg ctgacaagaa atagccgcat
     4921 cggaggtatc ttccgccaga cccaatcccc ctttaatacc acctgttttc gcccggctca
     4981 cataacaggt gacatttttc acatcgggat cgtcaaaggc ttccacaaca attttgtgat
     5041 ccgggccgat cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg
     5101 gccccagcat tatcaggctt aaagacagga tcaaatgctt gtatttcata ttgttaccat
     5161 ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa
     5221 aatctcaata cttttatttc cgtcaagccc taaaacatac tctactaaga aaaaaacact
     5281 gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt
     5341 gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct
     5401 ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc
     5461 caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat
     5521 tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct
     5581 ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa
     5641 gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat
     5701 tcgcccgccg ctacgtctgg gtgaattcac aatgccagag cacaaatttg tcaccctgga
     5761 agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga
     5821 tttccgccat gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg cgccgaccat
     5881 tccgccagtg ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgacgaaca
     5941 agaggtattc tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg
     6001 gcatccggtg atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac
     6061 cggcgtgcag gagtttatcc tgacggtata cggaacgtgc atgccaatgc tcaacctgac
     6121 gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagacgcca aagcgggaga
     6181 tcgcccaatt aatctgcgct gtgaactgct gattcctatc cgccgttaac gctgcagctc
     6241 atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga cccagccaga
     6301 cgccagccac aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa
     6361 gcggcgctct gcccatgctg gtaatccgag aatcgtactc accagacatc ccagtgcaat
     6421 accatggctc accagcagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc
     6481 cgcattgacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac
     6541 ggtgccattg accagctgcc gacgccagtt ctcttcttct tcggtcagag aatcgatatg
     6601 tcttttttcc agcacaccca tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca
     6661 gccgcaggcc tgggcgatga tttccgccgt acgccgggtg cgtcctaaat cgctactgat
     6721 aatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc
     6781 tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt
     6841 ttcaccgtgg cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg
     6901 ttaattttct gagtaatgct gattatgcac caagttatct gtgcgaccac caatcccgct
     6961 aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt
     7021 gcatccgttg ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc
     7081 gctggcgcac gaaatcgggt agcaaatgcc cgccgtttac ttccagaggc tgatttttgg
     7141 gtggcgattg aagctggcat cgatggtgac agcactttca gttgggtggt gattgaaaac
     7201 accagccagc gcggcgaggc gcgttcggct actttgccgt tacctgcggt gattctgcag
     7261 aaagtgcgtg aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag
     7321 attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc
     7381 gtctatcacc aggcggtgat cctggcgctg agtccgtttc acaatgccgt gtatcaggca
     7441 ttgtaggacg gataaggcgt tcacgccgca tccggcattc ggtgcacgat gcctgatgcg
     7501 acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag
     7561 ccactggcgc agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc
     7621 gatgcccgcg ccgagttcat tttttaactc acgctgactc atttcgccac gcaacagctc
     7681 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
     7741 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
     7801 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat
     7861 atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt
     7921 acgatagcat atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc
     7981 aacgtcggtt tatcccccat gaaataacgg tagtaagcgt cataagccag cacgttcttc
     8041 acataaccgc gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc
     8101 ccggcgctgt tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag
     8161 gagaaaatac ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg
     8221 ttgatattcg tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac
     8281 atcttcaccg tatgggtcgc tgtaccaggc ataatctgca tcaggccgct ggcccctacc
     8341 ggtgatttca ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt
     8401 tgcgggatct ctttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat
     8461 cgctcttcca gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac
     8521 cattggttat tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc
     8581 accagattgg cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc
     8641 acgcgcgcca tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca
     8701 atcttcagct catactcttc tccgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc
     8761 tgttgcatga gttgatgcaa aatctctttc gcttcagctt cacgtccgcg ttccagcaat
     8821 aaatccacct gccagtaacg ccattcatct ttctcttttg cttccatcgg cagacgcgcc
     8881 agccaggtat tcaggccgcg acgatcgccg gtgccaagtg ccattcgcac acggcgttca
     8941 atcaacgacg tggattgcga gcgcataatg acgtcatcgc gccatttcgc ctgctcgtcg
     9001 gtgacatcgt tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct
     9061 tcattaagct gctgcacctg ggcaagcgac gggatcatca gccgtgcatt ttcagcatcc
     9121 tgccgcgcca cactggcaaa cgccaccgct gccatttgac gggtaaaatc ggtcgctccg
     9181 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgag
     9241 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccagtgtta
     9301 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
     9361 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
     9421 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
     9481 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
     9541 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
     9601 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcagatca
     9661 tcggtgatct ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc
     9721 atttgttcga ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacgg
     9781 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
     9841 ccggcagcca acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag
     9901 tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc
     9961 gtttacgcca cgttacggac acttttttac ttttactgcg aaggtgatcg accaactggg
    10021 attaggcggg gaaaaaggct acacttcgcc tttgaaaact catcatcacg ataaacatag
    10081 aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc
    10141 cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg
    10201 gtgtcctggg tctgaacggc gcgggtaagt ctaccctgct gcgcattatg gcgggcattg
    10261 ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt tacctgccgc
    10321 aggagccgca gctgaacccg gaacacaccg tgcgtgagtc cattgaagaa gcggtttctg
    10381 aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg
    10441 ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg
    10501 acggtcataa cctgaacgta cagctggagc gtgcggcgga tgcgctacgt ctgccggact
    10561 gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg tcgcgtagcg ttgtgccgcc
    10621 tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg
    10681 aatccgtggc ctggctggaa cgcttcctgc acgacttcga gggcaccgtg gtggcgatta
    10741 cccacgaccg ttacttcctc gataacgttg caggctggat cctcgaactt gaccgcggtg
    10801 aaggtattcc gtgggaaggc aactactcct cctggctgga gcagaaagat cagcgcctgg
    10861 cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg
    10921 tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag
    10981 agctgaacag caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg
    11041 gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tacggtgatc
    11101 gcctgctgat tgatgacctg agcttctcga tcccgaaagg ggcaatcgtc gggatcatcg
    11161 gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat ctctggtcag gaacagccgg
    11221 acagcggcac catcacttta ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg
    11281 actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg gatattatga
    11341 agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg
    11401 ttgatcaggg taaacgcgtt ggtgaacttt ccggtggtga gcgcggtcgt ctgcatctgg
    11461 cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg
    11521 atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg
    11581 ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacatcctg gactaccagg
    11641 atgaaggtaa agttgagttc ttcgaaggta actttactga gtacgaagag tacaagaaac
    11701 gcacgctggg cgcagacgca ctggagccga agcgtatcaa gtacaagcgt attgcgaagt
    11761 aatgcgtaaa atgccggatg cggcgtgaac gccttatccg gcctacaaac caagaaattt
    11821 caatacgttg tgaatctcca ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag
    11881 caccgaatgc cggatacggc gtgaacgcct tttccggcct acaacagcct tagtatccgc
    11941 aatttcgtag cgggtcaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc
    12001 ttccagagag gttttcaact catcaaaaaa cttattttat acctcgcctt tgagccgttt
    12061 catcgcggtt atctctgctc ccccatcatc tcccgcacca gctccacgca gcgcaggaaa
    12121 cggctgtcat aatcgtcctc ttcaacccgc acgaattcga tattgttctc ttccagcatc
    12181 tccaccagca agttctggaa ctctttgcga tccaccgaac tgccgaggct gcgtaaacca
    12241 tccgccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca
    12301 atcaacgcct gtacgaacgg atgctcacgc ccttcgtact ttttgcagaa cgcctgggta
    12361 gtgacaaaat cagtgtcaat aaacgccact ttattggcat atttcactgc aaaatcaatg
    12421 tattgtgcgt ggcccagcgc gattttatcg taatcggaat actgcaatgc gatctcatca
    12481 ccgccgaggt gtgaaaagac ataatcgcga ccatattccc acgcactggt ggtattgaag
    12541 atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccacc aaggatcgcc
    12601 acggtacgta caaagaacgg cttcacttcc gtaggaatat attcccagta gcggaacggg
    12661 ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gtttcggatc gaccagcacc
    12721 gtatcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga ggtgtagatc
    12781 aggtccggct gaatcccctt ttcagccata aactttttga tgccgttgct ccacacatcc
    12841 cagccgtgcg gatacggctc catgccctct tcgttgaaag catgaatgcg aatatttttc
    12901 tgatatttaa aagtttgtaa taaccagcgc agacgatccg gcaccgtggg ctgctgcgac
    12961 atggcgctgt cttcaaacaa cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc
    13021 agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg
    13081 tagaacttac cgaatacgac accaatggtt ttcttctgtc ggggaaattc aagccccaaa
    13141 aaacggtgca acgcctccag cttttgcgcg ctggggcttt taattttggc attcagtaac
    13201 tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc
    13261 tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatctctc
    13321 attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca
    13381 tcccttccgg cgcttttttc ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt
    13441 gtttcgccgc ttctgaaatt cgttcctgac cgctgggcac cgggcggatc tcccctgcca
    13501 gcccgacttc accaaacacc accagatcct gtggcagcgg tctgtcacgc aggctggaaa
    13561 ccatcgccag cagtaacgct aaatcggcac tggtttcggt taccttcacg ccgccgacca
    13621 cgttcacaaa tacatcctga tcggccattt gcagaccacc gtgacggtgc aagacagcca
    13681 gcaggattgc cagacggttt tgttccagcc ccactgccac gcggcgcgga ttcgccatca
    13741 tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct tcccacacca
    13801 ccatcacgga gctgccggag gtcacttcat ctccgcgact taagaaaatt gccgaagggt
    13861 tgctgacttc acgcagcccc tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc
    13921 cgaagcggtt tttatggctg cgcaaggtgc gaaaacggga gtcggcatca ccatccagaa
    13981 gcaccgaaca gtcgatacag tgttccagca ctttagggcc agccagcgag ccatctttgg
    14041 ttacgtgccc aaccatgaca atcgccacac cgcgcgtttt ggcgaagcgc gtcagataag
    14101 ccgccgtttc acgcacctgc gccacgctac caggcgatga ttgtacatcc gccatatgca
    14161 tcacctggat cgagtcaatt accatcagtt tcggttgctc ttcttcggca atcaggcaga
    14221 tctgttcgat gctggtttcc gacaacatat tgagattgtc agtcggcagg ccaaggcgat
    14281 gagcgcgcat tgccacctgt tgcagcgact cttcgccagt gacatacagc gttttcatct
    14341 gctgggccag tttgcacagc gtttgcagta gcagcgtgga tttccccgca cctgggttac
    14401 cgccaatcag aatggcgctt cccggcacca cgccgccgcc tagtacgcgg tcgaactctt
    14461 taaatccggt ggaaaaacgc ggcagctctt caaggctgat atcggagagt ttctggactt
    14521 ttgccacccc ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc accgttggcg
    14581 acgcagcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct
    14641 gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg
    14701 ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg gcgggcgagc aattacttct
    14761 gattcaggct gccagagagg atgcagaata cccccatcag gtcagcgtga cggatggtga
    14821 cttccgcctt ttcattcact tttggcttgg catggtaggc aatccccagc cctgctgctt
    14881 tgatcatcgg caggtcatta gctccatcgc caatcgccac ggtctgcgcc agcgggattt
    14941 catactcctg cgcgaggcga gtcaaagttt tcgctttgta ctgcgcgtct acgatgtcgc
    15001 cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggcgg
    15061 tcaggcgcag cttgtcgcgc agatattcag caaagaaagt aaagccgccg gaggcaatcg
    15121 ccactttcca gcccagcgtt tccagcttga gcaccagttg cgttaagcct ggcatcagcg
    15181 gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacac
    15241 ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacttccg
    15301 ccaccatctc gcccgttccg gccagtttgg caatttcatc aatacattca atctggatgg
    15361 cggtggaatc catgtccatc accagcaaac ccggcgtgcg caggtgcggg attttcccca
    15421 gcggggcgac atccagttgc gcttcatggg ccaggcgcgt agcccgtgcg gtgagtgaac
    15481 ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatcgccg
    15541 cacccagttt gctctggtat tgggtcagac gctgtttatc cagcccacga ccatacagca
    15601 gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa
    15661 gaggcagacc cggccataaa gagacatctt caggcaggtc gcaccaggta atgttaggca
    15721 ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc
    15781 cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa
    15841 actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc
    15901 actgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta atccacagct
    15961 ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg
    16021 taccgactca ccagatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag
    16081 ccgcatcctc gacgcgggtg tgtatgacga acaaggcgat cttatcgccc gttcaggcga
    16141 aagcgtcgaa gtgcgcgacc ggctggcgct cgacggtaaa aaagcaggcg gctattttaa
    16201 ccagcagatt gtcgagccaa ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac
    16261 actcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt
    16321 acgcctgatg ttgctgctct cactggcaat cggcgtagtg ctgacccgca cgctgctaca
    16381 gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagca aaccggtgcc
    16441 ggaagaggaa gaaagcgaga aaaaagagtg acccattact acaagaaagg aaatcgttat
    16501 gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtgga
    16561 agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc
    16621 cgacacggta gtgattggtc gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat
    16681 ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga
    16741 tctcggcaat acctgcttta cctttatggc tggcaagccg gagtacgata aaaccatctc
    16801 tacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag cgtccggacg
    16861 taacgatctg gtggtgaaaa cagtcgaagg cgaccgcaaa gtctcaggct cggcctatcg
    16921 cgaaaccaaa gatcgtggct tccaccacgg cactttgctg ctcaatgccg accttagccg
    16981 cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacctcagt
    17041 gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg atcacccatg agcaggtttg
    17101 cgaggccata accaaggcct ttttcgccca ttatggcgag cgtgtagaag cggaaatcat
    17161 ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc gtcagagtag
    17221 ctgggaatgg aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttag
    17281 ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt
    17341 gtttaccgac agcctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg
    17401 cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga
    17461 ccaggaaaaa gagctacggg agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc
    17521 tgattaaaat accaaaggaa gggaatatga tcgatctgga aaaccaggag cgggagatta
    17581 tcaatttaat gttaagccag cgtatttcct ggctagccgc cgtgcgtatc aggcataaat
    17641 tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat taattctcta aaatagatcg
    17701 aaaagacgga acggcttagc tcaaacatca aaagcaaaat gggagaaatt tatggttgtc
    17761 caccagagtt gctgatatgc ccttcctgga tgacggctga acataaatga atagattcgt
    17821 gaatagatac ttgaatgtat tttatttttt atccatatga aaaataatag taaatctata
    17881 aatcagttgc tattgatgaa tagatactaa aaagccggag cagtccccca ctccggctta
    17941 acacgacaat cgcctttcag cgaaacacaa ttactcttta tcgcccagca gaacggattc
    18001 cagtgcgatt ttgatcatgt cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc
    18061 gtgagtgcgg atgtggtcag acacagtgca gatggtcagg gctttcgcgc caaattctgc
    18121 agcgacgccg tagataccag ccgcttccat ttccacgccg aggatgccgt atttttccat
    18181 cacgtcgaac atttcgccgt ccggagagta gaacaggtca gcggagaaca ggttaccaac
    18241 gcgagcatca atgcccagtg ctttagccgc gtctaccgcg ttacgcacca tgtcaaagtc
    18301 agcgatagcg gcaaagtcat gatctttaaa acggatgcgg ttaactttgg aatcggtgca
    18361 ggcacccata ccgataacga cgtcgcgcag ttttacgtgc ggcagaactg cgccacagga
    18421 acccacgcgg ataattttct tcacgccgaa atcggtgatc agttctttgg tgtagatgga
    18481 gcaggacggg atacccatac cgtgacccat tacggaaatt ttgcggcctt tgtaagtacc
    18541 ggtgaagccc agcataccgc gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc
    18601 agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa actacgtcag cgaaatcgcc
    18661 catttctgca ttaatgtgtg gggtagccat tgttttatcc ttatcaacat gttttcaccc
    18721 tctccctgag ggagagggcc ggggtgaggg catcaagccg cacccattat cagaacatgg
    18781 ctttgccata ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg
    18841 cgaaggtttc acggtgaccc agtgagcccg gttttacttt cgggccgtaa accagtaccg
    18901 gaatgtgttc acgcgtgtgg tcagtaccgg tccaggtcgg atcacaaccg tggtcagcag
    18961 tgaggatcag gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga
    19021 acagctccag acccacagca taaccggcga cgtcgcgacg gtggccccag gaagagtcga
    19081 agtcaacgaa gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg
    19141 tggcgtcaaa cagcgcgtcc aggccagttg ctttcacttt cttggtgata ccgcagttgg
    19201 cgtagatgtc tgcaatttta ccgacagaaa ccacctgacc gtgtttttca tcaaccagtt
    19261 tctgcagcac ggtcggtgct ggcggctcaa cagccaggtc gtgacggtta ccggtacgct
    19321 ggaagttacc ggctttgtca ccgataaacg gacgagcgat aacacgaccg atgttgtagc
    19381 cgccgttggt cagctcttca cgggcgattt cgcacagttc gtagagttta tccaggccaa
    19441 aggtttcttc gtggcaggca atctggaaca cagagtcagc ggaggtatag aaaatcggct
    19501 tgccggtttt catgtgctct tcgcccagct gatccagaat gaccgtaccg gaagagtggc
    19561 agttaccgag gtaacccggc agattagcgc gttcaaccag tttatccagc agctcttgcg
    19621 ggaagctgtt ttcgtgatcg gagaaatatc cccactcaaa tagaaccggg acaccggcga
    19681 tttcccagtg accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg
    19741 cgccgataac ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt
    19801 gtgctttcgc cagccccaga cgggtcagat ttggcagatt gagcggacct ttacgaccgt
    19861 tatcagcttc gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt
    19921 caccaaagcg ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa
    19981 atgcacgttt catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat
    20041 accgttattc gctgatacgg cgatagacag ttggtgtgct ttccggtgct ttatcggcaa
    20101 gtttaattgc cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcgt
    20161 ggataaccgc cagcggacgc tgaccgtcta cctggtcgcc caaacgcgcc atatcagtaa
    20221 agccgacgct gtaatcaatg gtgtcagatg cctgacgacg accgccgccc attgcaacca
    20281 ctgccatccc cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga
    20341 ctgctttcgt cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggttg
    20401 ggcctttttg tgccgctacc atacgaccaa agacttctgc tgctttaccg ttgtccagca
    20461 ctgcttgcaa tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagataagca
    20521 tctccacgca cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca
    20581 ggaactgcac cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat
    20641 tcatgtcggt gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg
    20701 cttcggcaag ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca
    20761 cgtccatcac cagcgcgtcc agaccttccg caagtttctt cgccagaata gaggcggtga
    20821 tcagcgggat ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatccg
    20881 ccggagccag tgagctggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc
    20941 ggaaacggtt gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg
    21001 taccgccagt atgaccgagg ccgcgaccag agatcatcgg aatatagccg ccgcaggctg
    21061 cgaccatcgg ccccaacatc agcgaagtca catcgccgac gccaccagtg gaatgtttat
    21121 caacaatcgg gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca
    21181 tcgccatggt cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg
    21241 cgagggcggc aatctgccct tcggagatag tgttgtcgcg aatgccgttg ataaagaaac
    21301 gaatttcttc atcgctcagt gcatgaccat cacgtttttt acgaataatt tcttgtgcga
    21361 gaaacaaggt aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg
    21421 ccttatccgg cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg
    21481 taaagcatct tacttagtag ctgctggcgc tcttaccgtc accgtggccc agcgctttca
    21541 acaggcttgc cagcaggctg gaagcaccaa agcggtagtg acgcgcatct gcccagtcag
    21601 caccgaacag ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc
    21661 cgcccgctgg tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca
    21721 tgatgcgcgc gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga
    21781 agtccgcacc cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca
    21841 gttcgccggt ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac
    21901 aggctttcac caggtcaaaa ccaacctgct cgttacccgc catcagcgcg cggtacggga
    21961 acacaacgtc aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga
    22021 tttcgatgtc atcgttaccg tgtgggaagt tggttaccgt agcgattcgg atttccgggg
    22081 tgccctgctc tttcagcgtt ttgcgagcaa tcgggataaa gcgaggatag atacagatag
    22141 cggcggtatt gccgaccggg gttttggcct gatgacacag agcaattact ttctcgtcgg
    22201 tgtcgtcgtc attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt
    22261 tcagatcagt catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt
    22321 tagtattcta acatctactc cgcaacacac ttcgatatac atcacaatta aggaaatcta
    22381 cttacaagtt tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg
    22441 cgtcagtggt ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc
    22501 gcatcacctt cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa
    22561 caacgttgcc agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt
    22621 ctggcactgg aagcagaaaa caatctgcct tatccgcagc ttcctgccga agcccgccgc
    22681 gcgctggatg agggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc
    22741 tatgtcttac ccgattacgc ccgttttctg gcgaacggtt ccgaatggct ggagctggaa
    22801 ggcgcgaaag atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg
    22861 tcggtcacat cgatgccggt ctacctgggg caactggatg cgttgttgca accatatgtt
    22921 agaattctaa cacaagacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac
    22981 agaaccctgc cagacgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc
    23041 cgcgcgatct tacgtgcaga cgcagagttg aagcaggttt caccgaacct gacctttatc
    23101 tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa
    23161 tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc
    23221 tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta
    23281 cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc
    23341 actctaccgc actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc
    23401 tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct
    23461 gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt
    23521 aagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat
    23581 cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa
    23641 aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc
    23701 gcgcgtttgc cgtatggtga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg
    23761 catcatgctt attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa
    23821 cgtaatccgc aggcgctggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa
    23881 tttactgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg
    23941 tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa
    24001 gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag
    24061 cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg
    24121 tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac
    24181 gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca
    24241 gattgttgac ggcaaagtgg tgtggaacgc tgtggtttgc gagcagtgtg atacctgcct
    24301 gaagaggtgt ccgcaacatg ccacgccaat ggcgcaatcc atgagcgtgg acgaagtgct
    24361 tagccatgtc cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc
    24421 cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact
    24481 gcgccacctc acctgcctgg tggacagtaa cggcatgttg agcgaaaccg gctgggaaaa
    24541 attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcgtggggga gcgaatgtca
    24601 tcaacactta accggacgcg ataatcagca gattaagcgc agcatctgtt tgcttgcaga
    24661 gcgcggcaag ctggcggaac tgcgtttgct ggtaattcca gaccaggtgg attatttgca
    24721 tcacatcgat gagctggcga cgtttatcaa gagacttggc gatgttccgg ttcgcctgaa
    24781 tgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga
    24841 agacgttgag ccgttggctg atgcgttaaa ggtgcgcggg gtgagccggt tgatatttcc
    24901 ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcatg
    24961 ataagacgcg acaagcgtcg catcaggcat tatgcgccga ctgccggatg cggcgtgaac
    25021 gccttatccg gcctacggtt cggcacagac ttgtaggcat gataagacgc gtcagcgtcg
    25081 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt
    25141 cggcacagac ttgtaggcat gataagacgc gtcaagcgtc gcatcaggca ttgtgcgcca
    25201 actgccggat gcggcgtgaa cgccttatcc ggcctacggt tcggcacaaa cctgtaggca
    25261 tgataagacg cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga
    25321 acgccttatc cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg
    25381 tcgcatcagg cattatgcgc cgactgccgg atgctgccta cgaagggtag gctacccact
    25441 aacgctaaac aacgcatacg tattattaag caacacttcg gcaatctcat cttctggttc
    25501 cgggcgcaac tcgcaaagca cgtcaaacac ccgcacagcc tgttccggtc ggttaggctg
    25561 cccctgaaag ccgttgagcg gcatatccgg cgcgtcggtt tccagcaaca acgatgccag
    25621 cggtaatttt gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc
    25681 tacgccgatt ttgtagccca gctgtacaaa ccgctcggcc tgttgcaggc tgccggaaaa
    25741 accgtgaacc acgccagtgc acggtaaatc gtgacgttta agatgcatcg ccagtttgtc
    25801 gtgtatacgc cgggaatgaa ggatcaccgg cagatcgtag cgtttcgcca gtttcagctg
    25861 ttcgtcgagt aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc
    25921 gatctccccc accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgatc
    25981 aagagacaca tcgctatgtt tttccaacat ccccggatgc aaccccaatg cggcatacag
    26041 cggttgatat ttttccgcta acgcctgcac gcgggcaaaa ttttccgcct ctgtcgccgg
    26101 aacaatgatc ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc
    26161 actaaacggc gggaaatcaa aatggcagtg ggtatcgata aaacggcaaa tcaagccaga
    26221 tcctcattgt taaatgtcgg gtcgttcgcc tgcggagcat cgctcacttc ggcaaccagc
    26281 gtatcgttgg cgactggcgc aggcggaata acgatcgatt ccggcgtcac cactggcacc
    26341 agatggcggg taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa
    26401 cgaccgcata aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc
    26461 gcgatactat gtaatggctt cggcggataa atttcgaata tccgcagctt gcccggtggt
    26521 ttctcaataa attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc
    26581 agcggctgca ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg
    26641 tagtacattt gtgacggcac agtgcgaatg acgaccaacg ttttagcgcc ctgccttgcc
    26701 gcctctttaa ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc
    26761 gacactccgc tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag
    26821 ttttgtttgg ttggtaaaaa gtaattaggc gcgtagtcat cctgacgaca ggcgcacata
    26881 taaaacgatt tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg
    26941 cttgcggtgg cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc
    27001 aatggatcga aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg
    27061 ggctgattgc agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga
    27121 tcgaaaggat taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca
    27181 cgctgtccgc cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac
    27241 aacggcgcaa tattaccaag cgttacaggt attcgctgcc ccacctctgc cttcctgttt
    27301 ttgttgtgaa agaatgacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag
    27361 tccgctggac tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg
    27421 gcccatgaac aaactcacca ggaacagaat aatcccgacg acaaagacaa ttttagctgc
    27481 gcctgcagcg gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac
    27541 cagaaatatg atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc
    27601 ttgatatgag cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc
    27661 ccgacaaagt tcgggaggac gaattacgac gaattactta gttttcagat catttttaac
    27721 gcttttcaca ccatctaccg ctttggcgat actttcagca cggtcgctct gtgcctggga
    27781 atcgacggta ccggagagct gaaccacgcc gtcggtggtt tcaactttca catgacggga
    27841 agggacgata tcgtccgcca gcagtttggc tttgatttca ctggtggtgg cggtgtcacc
    27901 cgcgtagccc ttcacagagc cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga
    27961 ggtcacccct tcaacgcctt tcgccacttt cactgcctct tcggcctgag cctggctttc
    28021 aacgaaaccg ctcagggtca cgactttttg atcggttttg acagagatat cggtgctctt
    28081 gatgttgtca tgatccacca gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat
    28141 gaaattaccg actttattca tagagctatc gactttttgc cctgcgcttt cattggtagt
    28201 ctgtgcgttg ttttccgcgt aggcagagcc ggtcgcgacg gcagaggtca acattacagc
    28261 cagcagagtt ttcgaaatct tcagtcttgt catagtcatc gatttattcc tgtatgtttg
    28321 ctcgtaattt gagcctggca acacgaggtt gcattgctga atagggagag acttcaccct
    28381 ctacagaagt caatggtcgc catcacaaaa gcgatgggtg atgaataacg accattacag
    28441 cctctgaatc agttattaat atcggtagaa tgacaatcga cggctttaga tactgatatc
    28501 tacgcattga acggtattta acgccgtcag aaatgtcatt actttgttaa atatagatca
    28561 caattttgaa accgctcggg atatcagcga aaacataagc aaaagtgaat gttttaagaa
    28621 cattccgtaa gcggctaata aggaagggaa attgacaggg cgcagcggct accgcgccct
    28681 ggaggcaaga attaatgctc gcgggtctgg tggaactgaa cgtcgggata acgttcctgc
    28741 gccaggcgca ggttgaccat gctggtagcg atgtaagcga ggttatcgcc gccatcaagc
    28801 gccagttggc tttcgttctt acgcttgaac tcttcgaatt tcttcgcgtc cgcacattct
    28861 acccagcggg cagtggcaac gttgactgat tcatatactg cttcaacgct gtattcgctc
    28921 ttcaggcgcg ataccaccac atcaaactgc agcacaccaa cagcaccaac gatcaaatcg
    28981 ttgttggaga tcggacggaa cacctgcacc gcgccctctt cggaaagctg taccagccct
    29041 ttgagcagct gtttttgctt cagcggatct ttcaggcgga tacgacggaa cagttctggt
    29101 gcgaagttcg gaataccggt gaacttcatc atctcaccct gggtaaaggt gtcgccgatc
    29161 tgaatagtgc cgtggttgtg caggccgagg atatcgcccg gatacgcttc ttcaacgtgc
    29221 gaacggtcgc ccgccataaa ggtcagcgcg tcggagataa ccacatcttt cgcagtacgc
    29281 acctggcgca gcttcatgcc tttttcatat ttaccggata ctacgcgcat aaacgccacg
    29341 cggtcgcggt gtttcgggtc catgttggcc tgaattttaa atacgaagcc ggtaaatttg
    29401 tcttcgctcg cctctacggt acgggtatca gtctgacgcg gcatcggcgc aggagcccac
    29461 tccaccaggc catccagcat atgatcaacg ccgaagttac ccagcgcagt accgaagaat
    29521 accggagtga tttcgcccgc aaggaacaat tctttgtcga actcgttaga cgcgcctttc
    29581 accagttcca gttcgtcacg tagctgctgt gccagatctt caccaaccgc agcgtcgaga
    29641 tccgggttat tcagcccttt aacaatacgg acttcctgaa tcgtgtggcc tttaccgctc
    29701 tgatagagat aggtttcgtc tttataaagg tggtaaacgc ctttaaacag cttgccgcag
    29761 ccaatcggcc aggtgatcgg tgcgcagccg attttcagct cgttctcaac ttcatcgagc
    29821 agttccatcg ggtcgcggat atcacggtca agtttgttca taaaggtgag gatcggcgtg
    29881 tcgcgcagac gggtaacttc catcagctta cgggtacgat cttcaacacc ttttgcagcg
    29941 tcgataacca tcaggcagca gtccaccgcc gtcagggtac gataggtatc ttccgagaag
    30001 tcttcgtgcc ccggggtgtc gagcaggtta accaggcaat cgtgatacgg aaactgcatc
    30061 acagacgtag taatggagat cccacgctgc ttttccatct ccatccagtc cgacttagcg
    30121 tgctggttgg aaccacggcc ttttactgta ccggcggtct gaatggcctg tccgaacagc
    30181 agcaccttct cggtgatggt agtcttaccg gcatccgggt gagaaataat ggcaaaagtg
    30241 cggcgcttcg ccacctcttg caaataagga gacaacgtca taattctttc ttcttgagta
    30301 agcggcatcg cgccgcgctt gttgaaaacg aaaaattgcg gctattttac ccatcaacgg
    30361 gggggaggca atcagtgttt acacaggagc tgctccagtt cgtgcaacga agaaacggtc
    30421 caggtgggcg cgatgccttc tggttgctcg cgattgtgcg cattcagcca gcaggtcgca
    30481 agcccggcgt tgatgccacc gagaatatcg gactcggcag tgtcgccaac catcagcacg
    30541 cgtgaacggt caggattgcc cgcctgttcc agcgcataat cgaaaatttt cttattcggt
    30601 ttggcaacgc caacttcttc ggaaatcacc agcaaatcga agtaatcacg caggcccgtg
    30661 cgttccagac gcacttgctg caaggcgcta aagccgttgg tgatgatgcc gattttggcg
    30721 ttgccacgaa tggcgttaag cagagaaacc gcgcccggca gcggcgtgca gatttccgcc
    30781 atcgcattaa taaaggcatc gttaagcaag cctgcttcaa ctttcagccg ttcggcccag
    30841 ctctcaaaac gcccgtgctg aagctgtaat gaagtgatcg cgccgttttg ataatccacc
    30901 cacagtggct tgttaacggc ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg
    30961 taatcaagaa acatccgctg caggccggtg aatgagtcaa aggtaaacag cgtttcatcg
    31021 gcatcaaaga aaatccagtc ccacttcatt attccacctt gtcttacata ctgattggca
    31081 acgccatgat gatggcgtct tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg
    31141 tcgcctcgtt aaagcctaaa ctttcgtaca gggcaatggc ggcagcattt gaggcacgga
    31201 cttccagcca tagtgtcgcc acgccgcgtt tttccagttc gtcgatcaga tgttccagca
    31261 gcacccttcc caatccctga cgctgatagt caggatcgac cgcaatattg aacaatgtag
    31321 cttcatccag caccacttgc gtaatcgcaa acgccgccat tttgccgttt tgcgttaact
    31381 gaaagttgag ataacgctcg ccctggttgc tggcaaaggt tttttcactc cacggaaagg
    31441 cgtgggcgcg ttgttcaatg tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa
    31501 tcgtgttcat atgtgcaaat ctgttgccat aacgcggcgc gtgccgttgg gtttgcccgt
    31561 aattcggtga gcgccggtga tgccacctga gcgccttcca gtgatagcgg ttcgtcagta
    31621 cccaaccgcc agctgttgca gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc
    31681 agttgcagca cttgatcggg gctgacggtt aatgcgcgca gaacatcgct cactaaagga
    31741 tcggtcaggg cgggaagatc gtttgccacc atcaccagac ggacgtgtgc cgggatcgca
    31801 atggcaatct ccccctgcaa cgcgccaggg cgacgcagcg accactgggt aatgcccagt
    31861 tgctgtaact gccagtctcg tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc
    31921 aaatatagca aattcgtcga taccgcgcca acatatggct ataatcgccg ccagtatcaa
    31981 ttgaggagca ttccatgtct gcatttaccc cggcaagtga agtcttgctg cgtcacagtg
    32041 atgatttcga acaaagccgt attctgtttg ccggagactt acaggatgac ctgcccgcgc
    32101 gtttagatac cgcggccagc cgtgctcata cccagcaatt ccaccactgg caggtattaa
    32161 gccgccagat gggggataac gcccgtttca gtctggtcgc cacggtggat gacgtcgcag
    32221 attgcgatac gctgatttac tactggccga agaacaaacc ggaagcccag ttccagttga
    32281 tgaatttact ttctctgctg ccagtgggga cagatatttt tgtcgttggc gagaaccgca
    32341 gcggcgtgcg cagcgccgag cagatgctgg cagattatgc gccgttgaat aaagtcgaca
    32401 gcgctcgtcg ctgtggcctc tattttggtc gtctggaaaa acagccggta tttgatgccg
    32461 ataaattctg gggcgaatac agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt
    32521 ttagccgcga cggtctggat gtcggtagcc agttgctgct ctcgacgtta actccgcaca
    32581 cgaaaggtaa agtgctggat gtcggctgtg gcgcgggggt gctttcagtt gcctttgcgc
    32641 gccattcgcc gaaaattcgt ctcaccttgt gcgatgtctc tgcgccagcg gtagaagcca
    32701 gccgcgcaac acttgcggcc aactgtgttg aaggtgaagt ctttgccagc aacgtctttt
    32761 ccgaggtgaa aggtcgtttt gatatgatca tctccaaccc gccgttccac gatgggatgc
    32821 aaaccagcct ggatgcggcg caaacgctga ttcgcggcgc ggtgcgtcat cttaatagcg
    32881 gcggcgagct gcgaattgta gcgaacgcct tcctgcctta cccggacgtg ctggatgaga
    32941 catttggctt ccatgaagtg attgcgcaaa ccgggcgttt caaggtgtat cgcgccatta
    33001 tgacccgcca ggcgaagaaa ggttaattat ctcgccgaat accgtgtcgg atgcggcgtg
    33061 aacgccttat ccgacctaca aaacctgcac gttagccctt cgtaggccag ataagacgcg
    33121 ccagcgtcgc atctggcatc tgcccccgca tgcctaccaa acctgcacgc tagtttcctg
    33181 atggacattt ttccagcaat tacacctccg gcgataatta actattgacg aaaagctgaa
    33241 aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg tatgcgaagg
    33301 tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt ccttacggac gtgggggttc
    33361 aagtcccccc cctcg
//
LOCUS       DAZPLM010000021        32839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPLM010000021 DAZPLM010000000
VERSION     DAZPLM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.021"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHVC7_000868"
     CDS             267..1925
                     /locus_tag="ACHVC7_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR2114984.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHVC7_000869"
     CDS             complement(1922..2878)
                     /locus_tag="ACHVC7_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR2114985.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHVC7_000870"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHVC7_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR2114986.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHVC7_000871"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHVC7_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR2114987.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHVC7_000872"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHVC7_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR2114988.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHVC7_000873"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHVC7_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR2114989.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHVC7_000874"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHVC7_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR2114990.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHVC7_000875"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHVC7_000875"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR2114991.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHVC7_000876"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHVC7_000876"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHVC7_000877"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHVC7_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR2114992.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHVC7_000878"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHVC7_000878"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR2114993.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHVC7_000879"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHVC7_000879"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR2114994.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHVC7_000880"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHVC7_000880"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR2114995.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHVC7_000881"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHVC7_000881"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR2114996.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHVC7_000882"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHVC7_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR2114997.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHVC7_000883"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHVC7_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR2114998.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHVC7_000884"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHVC7_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR2114999.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHVC7_000885"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHVC7_000885"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR2115000.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHVC7_000886"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHVC7_000886"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR2115001.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHVC7_000887"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHVC7_000887"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR2115002.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHVC7_000888"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHVC7_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR2115003.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHVC7_000889"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHVC7_000889"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR2115004.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHVC7_000890"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHVC7_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR2115005.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHVC7_000891"
     CDS             29803..30951
                     /locus_tag="ACHVC7_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115006.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHVC7_000892"
     CDS             31161..32591
                     /locus_tag="ACHVC7_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR2115007.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32839)
                     /locus_tag="ACHVC7_000893"
     CDS             complement(32592..>32839)
                     /locus_tag="ACHVC7_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2115008.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8031 c         8814 g         7936 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctattgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatcat
//
LOCUS       DAZPLM010000022        32634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPLM010000022 DAZPLM010000000
VERSION     DAZPLM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.022"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            120..262
                     /locus_tag="ACHVC7_000894"
                     /pseudo
     CDS             120..262
                     /locus_tag="ACHVC7_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="ACHVC7_000895"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="ACHVC7_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR2115009.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHVC7_000896"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHVC7_000896"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR2115010.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="ACHVC7_000897"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="ACHVC7_000897"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR2115011.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="ACHVC7_000898"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="ACHVC7_000898"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR2115012.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6658
                     /gene="hemX"
                     /locus_tag="ACHVC7_000899"
     CDS             5417..6658
                     /gene="hemX"
                     /locus_tag="ACHVC7_000899"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR2115013.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            6661..7857
                     /gene="hemY"
                     /locus_tag="ACHVC7_000900"
     CDS             6661..7857
                     /gene="hemY"
                     /locus_tag="ACHVC7_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR2115014.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8519..8656
                     /locus_tag="ACHVC7_000901"
                     /pseudo
     CDS             8519..8656
                     /locus_tag="ACHVC7_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(8764..9999)
                     /locus_tag="ACHVC7_000902"
     CDS             complement(8764..9999)
                     /locus_tag="ACHVC7_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR2115015.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10146..10222)
                     /locus_tag="ACHVC7_000903"
     tRNA            complement(10146..10222)
                     /locus_tag="ACHVC7_000903"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10186..10188),aa:Pro,seq:tgg)
     gene            complement(10265..10351)
                     /locus_tag="ACHVC7_000904"
     tRNA            complement(10265..10351)
                     /locus_tag="ACHVC7_000904"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10315..10317),aa:Leu,seq:cag)
     gene            complement(10372..10447)
                     /locus_tag="ACHVC7_000905"
     tRNA            complement(10372..10447)
                     /locus_tag="ACHVC7_000905"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10412..10414),aa:His,seq:gtg)
     gene            complement(10506..10582)
                     /locus_tag="ACHVC7_000906"
     tRNA            complement(10506..10582)
                     /locus_tag="ACHVC7_000906"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10546..10548),aa:Arg,seq:ccg)
     gene            complement(10685..12070)
                     /locus_tag="ACHVC7_000907"
     CDS             complement(10685..12070)
                     /locus_tag="ACHVC7_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2115016.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12261..13001)
                     /gene="wecG"
                     /locus_tag="ACHVC7_000908"
     CDS             complement(12261..13001)
                     /gene="wecG"
                     /locus_tag="ACHVC7_000908"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR2115017.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(13004..14356)
                     /gene="wzyE"
                     /locus_tag="ACHVC7_000909"
     CDS             complement(13004..14356)
                     /gene="wzyE"
                     /locus_tag="ACHVC7_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR2115018.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14353..15432)
                     /gene="rffT"
                     /locus_tag="ACHVC7_000910"
                     /gene_synonym="wecF"
     CDS             complement(14353..15432)
                     /gene="rffT"
                     /locus_tag="ACHVC7_000910"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR2115019.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15429..16679)
                     /gene="wzxE"
                     /locus_tag="ACHVC7_000911"
     CDS             complement(15429..16679)
                     /gene="wzxE"
                     /locus_tag="ACHVC7_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR2115020.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16681..17811)
                     /gene="rffA"
                     /locus_tag="ACHVC7_000912"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16681..17811)
                     /gene="rffA"
                     /locus_tag="ACHVC7_000912"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR2115021.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17816..18490)
                     /gene="rffC"
                     /locus_tag="ACHVC7_000913"
                     /gene_synonym="wecD"
     CDS             complement(17816..18490)
                     /gene="rffC"
                     /locus_tag="ACHVC7_000913"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR2115022.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18468..19349)
                     /gene="rfbA"
                     /locus_tag="ACHVC7_000914"
     CDS             complement(18468..19349)
                     /gene="rfbA"
                     /locus_tag="ACHVC7_000914"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR2115023.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19368..20435)
                     /gene="rffG"
                     /locus_tag="ACHVC7_000915"
     CDS             complement(19368..20435)
                     /gene="rffG"
                     /locus_tag="ACHVC7_000915"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR2115024.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20435..21697)
                     /gene="wecC"
                     /locus_tag="ACHVC7_000916"
     CDS             complement(20435..21697)
                     /gene="wecC"
                     /locus_tag="ACHVC7_000916"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR2115025.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21694..22824)
                     /gene="wecB"
                     /locus_tag="ACHVC7_000917"
     CDS             complement(21694..22824)
                     /gene="wecB"
                     /locus_tag="ACHVC7_000917"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR2115026.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22880..23926)
                     /gene="wzzE"
                     /locus_tag="ACHVC7_000918"
     CDS             complement(22880..23926)
                     /gene="wzzE"
                     /locus_tag="ACHVC7_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR2115027.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23938..25041)
                     /gene="wecA"
                     /locus_tag="ACHVC7_000919"
     CDS             complement(23938..25041)
                     /gene="wecA"
                     /locus_tag="ACHVC7_000919"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR2115028.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25281..26540)
                     /gene="rho"
                     /locus_tag="ACHVC7_000920"
     CDS             complement(25281..26540)
                     /gene="rho"
                     /locus_tag="ACHVC7_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR2115029.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26625..26726)
                     /gene="rhoL"
                     /locus_tag="ACHVC7_000921"
     CDS             complement(26625..26726)
                     /gene="rhoL"
                     /locus_tag="ACHVC7_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR2115030.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26867..27196)
                     /gene="trxA"
                     /locus_tag="ACHVC7_000922"
     CDS             complement(26867..27196)
                     /gene="trxA"
                     /locus_tag="ACHVC7_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR2115031.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27327..28592
                     /gene="rhlB"
                     /locus_tag="ACHVC7_000923"
     CDS             27327..28592
                     /gene="rhlB"
                     /locus_tag="ACHVC7_000923"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR2115032.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28726..30210
                     /gene="gppA"
                     /locus_tag="ACHVC7_000924"
     CDS             28726..30210
                     /gene="gppA"
                     /locus_tag="ACHVC7_000924"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR2115033.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30257..32278)
                     /gene="rep"
                     /locus_tag="ACHVC7_000925"
     CDS             complement(30257..32278)
                     /gene="rep"
                     /locus_tag="ACHVC7_000925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR2115034.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            32495..>32634
                     /locus_tag="ACHVC7_000926"
     CDS             32495..>32634
                     /locus_tag="ACHVC7_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115035.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         8229 a         8823 c         8186 g         7396 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 tgcacctgca cctgcaccgc aaggtgatac tccggcagcc gcgccgcaag gagaataacc
     6661 atgctaaaag tgttattact ctttgttttg ctgattgcgg ggatagtggt tggcccgatg
     6721 attgccggtc atcagggtta cgtgctgatc cagactgaca actacaatat cgaaaccagc
     6781 gtcacgggcc tggcgattat tttgattctg gcgatggtag tgctgtttgc cattgagtgg
     6841 ctactgcggc ggatcttccg cacaggcgca cacacccgtg ggtggtttgt cggacgtaag
     6901 cgtcgccgtg cccgtaagca gaccgaacag gcgctgctga aactggcgga aggcgattat
     6961 cagcaagttg aaaagctgat ggcgaaaaat gccgatcacg cggaacaacc ggtggtgaac
     7021 tatctactgg ctgccgaagc cgcgcaacaa cgtggtgatg aagcacgcgc caaccaacat
     7081 ctggaacgcg cagcagagct ggccggcaat gacaccattc cggtagaaat cacccgtgta
     7141 cgtctgcaac tggcccgtaa tgaaaaccat gctgcacgcc acggcgtgga taagctgctg
     7201 gaagttacgc cacgccatcc ggaagtatta cgtctggcgg aacaggcgta tatccgcaca
     7261 ggtgcatgga gttctctgct ggatatcatc ccatcaatgg cgaaagccca tgttggtgat
     7321 gaagaacatc gtgcaatgct ggaacaacag gcatggattg gcctgatgga tcaggcgcgt
     7381 gccgataacg gtagtgaagg tttgcgtaac tggtggaaaa accaaagccg gaaaacgcgt
     7441 catcaggtag cgttgcaggt ggcaatggcg gaacatctta ttgaatgtga cgatcatgat
     7501 actgcccagc aaattatcat cgatggcctg aaacgccagt acgacgatcg cctactgctg
     7561 ccgattcctc gactgaaaac caacaatccg gaacagctgg aaaaagtgct gcgccagcag
     7621 atcaaaaacg tcggcgatcg cccgctgttg tggagcacac tgggccagtc actgatgaag
     7681 cacggagaat ggcaggaagc atcgctcgcc ttccgcgcag cgctgaaaca acgtccggac
     7741 gcctacgatt acgcatggct tgccgacgcg ctggacagac tgcacaagcc ggaagaagct
     7801 gcagcgatgc gtcgcgacgg tttgatgtta acgttgcaga acaacccacc acagtagttc
     7861 cttctcaccc ggaggcaagc acctccggga cctttctgat gcataaaaaa acgcctgctc
     7921 ttattacgga gcaggcgtta aaacaggtct gtatgacaac agtgggtgct tcactcaacg
     7981 ttgtgtccat ggtgtctgat gaggcataag cgacatctgt cagtggacga taagcaccgt
     8041 aaacggctct gcgtcattcc ggagtttatg aggcactaag gcgaacataa gagatggaat
     8101 gagcatctac ccgtttatta tgccacagag aatcgggaaa taacatccct taacacttgt
     8161 tatgagataa ttctgtaatc ctctttgctt cctgagtgaa tatttaacct gagcttgatc
     8221 ctacacatac ttattatgaa tgataaaatt cattcaatta ataacacata tattaattgc
     8281 cgttaaaact aaaaaagcat taataatcaa cgcaatataa taaacccgcc ttacatatca
     8341 actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt ttctggataa
     8401 ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg agagggatct
     8461 caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag cattttgcat
     8521 ggaattttcg ttttcaccca aacttcttgt tgttgctgtc gccgccgctc ttcctctcat
     8581 ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg ccggatacga
     8641 tcacccaaac cagtatccac agaacgctgc aatttattga atttgcacga tcatgtaggc
     8701 cggataaggc gtttacgccg catccggcaa tcaaccgcag gcggccacaa aatggccgct
     8761 tttttactta ttcaccacca gcaaatgtgc ctgcataatg tcactggccg ggcgaccgtg
     8821 cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat aacgctgata
     8881 cccggcacac aaataattca gccccggttt gccgctggca tcgagcatga agcggtgttt
     8941 cgggcagcct ccccagcacg cttttaacac gttacaactg cgacactgcg ccggtaattg
     9001 cttaaattta tcttcaccaa acacctgctg ttgcggggaa tcgatcattt ctgcaattgt
     9061 ttgctgatgc atattcccca gccgatattg cggataaaca tagtgatcgc aggcgtaaac
     9121 gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc
     9181 acccggcgca ccgacaaaat tggcaaacgc ccattcgata ttcatcacga aaatcttgcc
     9241 gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga aattcaccaa attcatcggg
     9301 gcgcaccgac cattccgtca gttccccctg aatatcacca ggcgcatgca atttcagtcc
     9361 atcgcgggca gctgtttcat cagccagacg ctcgaccacc ggaataaact ggatgaattc
     9421 gactcccgca tcgcacaaaa aatcataaac ctgcaacggt tgctgcgcgc tggtgcggtt
     9481 gacgcagacc agcacgttat agtcgacatg atgtttttgc agcagcgtca gggcacgcat
     9541 caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt ggatctcagc
     9601 cggaccgtcc agcgataacc caacaagaaa atgattttct gccagaaatg cacaccattt
     9661 atcatcgagc agcacgccgt tagtctggaa gctgttactt atcttcctgc cagcaccgta
     9721 tttcgcctgt aactttacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc
     9781 gccctgccag gtaaaagcca cttcgttttg cggttcgctg gcagcgatat agtgacggac
     9841 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg
     9901 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccac tcggtttcgc
     9961 catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcactcctt gcctgaagtc
    10021 atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt acgggcgttt
    10081 attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
    10141 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
    10201 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
    10261 gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
    10321 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
    10381 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
    10441 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga
    10501 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
    10561 tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca
    10621 cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt gtgcgttttg
    10681 ctgcttattc ctccagttta tgcgctttcc cgtggcgatt aaggccaaaa actttataaa
    10741 tcgccgtcac cgccagcata aagatgatgc caacaaacag cgacatacgc gtatcttcat
    10801 taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccacg
    10861 ggaacaggat tgagcggaac ggatggctgg caatcgccgc tttatgtgca cgccgaaaac
    10921 gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt
    10981 agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta
    11041 gcagaatagc aatagatact gccacacccg caactggtac gccgtgacgg gaaactttcg
    11101 ccatcgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt
    11161 acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg
    11221 ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg ttgctgccaa
    11281 tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag aaaatcagga
    11341 tccgccacag cactttgcct acagcactgc gcagcgtcac ctgcggattc ttcgcttcac
    11401 cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata cacagcgcgg
    11461 tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga ttgctaaaac
    11521 caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca
    11581 tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc
    11641 gcaccgccgc cagattcgcc aacgccacca gcgccaccgc gatcaatgca ggtatccact
    11701 gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc
    11761 ccaccgccat ccacataaac cagtaagacc aggcggtgag ataaccaaaa aacgggctca
    11821 tataacgatg cgcataaacg gcgaacgaac cggtaacagg ttcaaggaac aacatttcgc
    11881 ccattgaacg catgatgaaa aagacgaaca gcccggcgat gatataggcc aacaatacgg
    11941 atggcccggc ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatagtgc
    12001 cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt
    12061 tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta
    12121 tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt
    12181 aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacggagtga ccactccgtc
    12241 gctttacaaa gagaggaaaa tcagaggttg ccggtgtagt gccagcgtaa ataacgcagc
    12301 aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagt
    12361 cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaagtc
    12421 ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc
    12481 tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt
    12541 tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc
    12601 acattccatt ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc
    12661 acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg
    12721 gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg
    12781 ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac ctcggcgtta
    12841 tcttcaatgg tcagcatttt ttcagcatta atggcaacca gcgttccctg cttaagctgc
    12901 ccgtcagcaa acagataatc gagggcgtgc tgcatatcac gccaaccaat caactgtaag
    12961 ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg
    13021 tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca
    13081 gcagttttgc gattatcaga catgcgccga agaccacgat aaaaaagact acgcgtgaga
    13141 caaacgaatc cagcccttca cgcgccagca cgatcatatt gaagatcgcc ccaaagcaga
    13201 aactgtgcaa tatcgcagcc ttatagcgat taggctcgcg gttgcccagc tcatacagcc
    13261 agtcgaacca tttgatgatc agaccaacca cgatcgcccc gagcgggatg aacaacgcgc
    13321 cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg gagtggttat
    13381 tcagcacttc ccaggtaaag tagttggctg agttcagcac catactcggg cgacccggcc
    13441 acagccagga agggataaag acatagaaat cgcggacaat tggagccagg ccctggaagt
    13501 cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggggag aaggtgtcgc
    13561 gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt ccatagcgtt
    13621 ttagcgccat ccagaacatg ccaacaatcc ccagcacgcc cgccgccgcc agcatccaca
    13681 acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg atgatgatat
    13741 tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaggcgacc gtgctgacga
    13801 ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc atcgccggga
    13861 tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggag aagatctgac
    13921 tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctta
    13981 ccagcgcgat acccatcagg atcacccacg taagattggt ctccacgcgg ttcatggtaa
    14041 acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg taggtgacat
    14101 agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaagatt tctggcggcg
    14161 cgacaccaac atcaaagcga aataccagca cgctggtcag cgggaagccg aagaaaaagg
    14221 tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa
    14281 accaggtcag cgtggcaata aacagcgtgc agagcagcca gaccacaaac aggccactga
    14341 attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg
    14401 tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg
    14461 cgcttcacgc acaatatcct cgttgagatc gtcggtagta aacagcaccg gcaaatgctg
    14521 ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg
    14581 aatcagtaag caaagcgtac caatgccctg ctggcgggca aaaataaagt aaccaagatc
    14641 gcactgacga agtagcgcca gataggcgtc aaattccagt ttttcgctca aaatttgcag
    14701 attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt acgcttcgtt
    14761 attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac
    14821 ggcgcgcaag gcagcaatat ggtcattgct gcggtcaccg gagttcccca ccagaatggt
    14881 cattttccct tcacgttgcc gatcattcgc catcgtattg agagaagggt ccatccgcgt
    14941 cgggaagtac agcagttcgc cccgcacctt tggatgcgtt ttggcaaaaa agctcaaatc
    15001 accgcgggtg gcaaatacac agccgactcg cttttgcgcc aggcgacgta gcgggtaaaa
    15061 aagcttatat ctcaagccac tggaaagctc atacaggtct gccccccaga tatgccagaa
    15121 aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg gattgaactg
    15181 accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc
    15241 cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat cacttaagcc
    15301 gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc
    15361 attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat
    15421 cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag
    15481 acgatataag ttgccatata tgcctgcgcc gcgcccagtg caccatgcgc agggattagc
    15541 cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat ataaaaccgc
    15601 agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt taacacatca
    15661 cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcaac
    15721 cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag
    15781 acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac
    15841 aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa
    15901 atactgctca ccccttgcca gatccccact tcatcccagc tatactgcgc cgccagcagt
    15961 ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta
    16021 aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg
    16081 atgacaccac gtttgatcaa catgatggcg gcaggaatca ccaccagcgc gggaatcagc
    16141 gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta gtacgcgaga
    16201 acgccaatca agctgccgac aatcagcgat aacgcattac ctgcggcatc gcgaaagcct
    16261 ttcatcagcg ccagtaacag gttgccccag gcgatcccca tttgcaccag cgccactaaa
    16321 cgcaccagcc cctgatagtc ggtattacca aacagtccct ggctgattgg cgcagctgcc
    16381 agcacaaaaa ccagcgccat cagcgtagag aaaccaagta ccatcgctga tgaagtgccg
    16441 accacgcggc gcagctgttg cggattatca tggtactggg caacgtattt ggttacaccg
    16501 ttaaagatgc cagctccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc
    16561 gccagcccaa gccccgccgg accaaatgac acagccagca acttaccgac cagtaacccg
    16621 gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata
    16681 tcaggagaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg gcgacaggtt
    16741 gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcacc
    16801 gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt aatggaacac
    16861 cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat caatatcccg
    16921 cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc
    16981 gatacgcccg gctttcgcca gaggcgctaa cgcatcgtag tagttttgcc acagcgccag
    17041 acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtacg cagcttgcag
    17101 atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga cctgaccacg
    17161 gaagaactgg ctgcggttag tgcccttttc acggatgatc tcggctcgtt cgattaacgc
    17221 tttatcgtta atcagcgtcg cgccgccttc accacccgcc gtatagtttt tggtttcatg
    17281 gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt aagtggacat
    17341 cacgccctga gcggcatctt ctaccacaaa cagattatgc tttttcgcca acgccataat
    17401 ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc
    17461 ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc gaacatccac
    17521 aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct
    17581 cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga
    17641 agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg
    17701 ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca ttgccgactg
    17761 catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc
    17821 tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg
    17881 cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc
    17941 agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc
    18001 gcgcatctgt cgcattgagt tcccgtaaag agacataacc gcgaataccg ccggacgccg
    18061 cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg
    18121 cataaaagcg tccgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa
    18181 atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc actacagcac
    18241 cactatcact gacattgttc acgggtagtg ccaaatcaac ttcaccttct accagggaga
    18301 atcccagctg ttgcagggca tccagttcac ccgtatttga tgccgcaatt ttggcctgca
    18361 cccgtgacca cggcgctaac acgtctggcg tcaggagcgg tgcttcagac gtaatgcgca
    18421 cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc
    18481 ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc taatgaactg
    18541 gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat ctcttccagg
    18601 caggcaatct taaagccctg gcgtttttct actgtctgca caaaggtact ggcttcaatc
    18661 aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag ttcaacggtc
    18721 aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc
    18781 gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata aagcccggtc
    18841 accgcccagt ttgacttcgg ctgttttggc ttttcttcca gcgagatagc gcggaaattg
    18901 tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca cctgatagcc aaataccgtc
    18961 gccccttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg accgaagaag
    19021 atgttatcgc ccagcaccag acaagaaggt tcaccgttga ggaaggtttc accgatgata
    19081 aaggcctgcg ccagaccgtc cgggctgggc tgttcggcgt attccagctg gataccgaac
    19141 tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg
    19201 agaatttcgc ggatatcggc caacatcagc accgacagcg gatagtaaat cattggctta
    19261 tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg
    19321 gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta gcctttcaga
    19381 cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc
    19441 agataccact gcaccgtttt acgcattcca ctttcaaagg tttcctgtgg cagccagcca
    19501 agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca
    19561 gcgacaaagg tgatcaagtc acgatagtgc accacgccgt gcggcttgtt cggagccagt
    19621 tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca
    19681 ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg
    19741 tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt
    19801 ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga
    19861 ccgtagttat tcgagcagtt ggtgataagc gtcggcagac cgtaggtccg cagccaggcg
    19921 cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg cgcatacggc
    19981 gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtggag
    20041 atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgtattcca gtaagcccgc
    20101 gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc tgccgggccg
    20161 tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatgc
    20221 tcagtgaata cgcgtgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag
    20281 cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt cagcttatcg
    20341 accactacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata
    20401 aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct ttggcatcga
    20461 cgacatactg ctgatggaca ttgtcgccat tgataacttt aaactgacta tgatcgacca
    20521 gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc
    20581 cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagagtt tcgccgctat
    20641 gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg tcatcaatat
    20701 tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag
    20761 ccgccaggca atcagccacc gccgctttca cctgatcgat aacccagaac ggtttgtgat
    20821 cgttcacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt gccacgataa
    20881 accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga agaatattaa
    20941 cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc tgatcggcac
    21001 aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg ttttcggtga
    21061 gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt
    21121 tgtacagttc gctggcgcgg gtcgaacaaa ccggcgtcat accaccaatc acgcgatcgt
    21181 ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag taagcaatgt
    21241 tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag atccggacgc atctctgcta
    21301 accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccagcg
    21361 cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg
    21421 gctcatgatc gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc gcttcaactg
    21481 gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat
    21541 caggctcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat
    21601 cgacaccaat cacctgtttt tgccgtgagg caaacgctgc tgctgtaggc agcccgatgt
    21661 aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa
    21721 cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat
    21781 agtttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt
    21841 atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct cagtggtatc
    21901 gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc
    21961 ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc
    22021 gatcagaatg acatttttca catgccccag aatgcgattg accggctctc tgacgttcgg
    22081 gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcaa tgtctgccaa
    22141 cgcgtggcag atttcttcaa agccacgacc gaaactttca cgcctgtgac cggtcaccag
    22201 aatcatcttt ttatcggggt cgataaacgg gtaatttgcc gccagttctg aacgcagcgt
    22261 atcgctgctc atcacctggt cacgcaccca taacagtgca tcaataactg tattaccagt
    22321 aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg aagtttcggt
    22381 tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca
    22441 cggcgaataa agatcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg
    22501 ataaaacgcc gccaggctgg ttgccagcgt cgtcgttgtg tcgccgtgaa ccagcacgac
    22561 gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac aggttatctc
    22621 tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag
    22681 tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa
    22741 aaaaggatct tttgccaacg catgcaccaa cggcgccatc ttgatggctt ccgggcgcgt
    22801 accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc
    22861 gctcaccgca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga
    22921 tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt
    22981 ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg
    23041 gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga tcaaaggccg
    23101 gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca
    23161 tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct
    23221 gctcagcaat tttcagcgcc tgttcaatac tgttcatccg gcggtcgtag atggctttcg
    23281 ccacctcttc ctgacgcttc acctgtgctt tcatctggat ggtacgcgcc gcccatgcgc
    23341 ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac
    23401 gtaacaggtt attagcgtca ggcgcggttt cagcaataag cttcacgctg tcattgaccg
    23461 cgcgggtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc agcaacgccg
    23521 catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc agccagaact
    23581 ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga
    23641 ccgatggttg gtcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct
    23701 gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc gccgtcgagc
    23761 tccactcctg acgagcaaaa aaagtatacg ccagcgcgat taacgcaaat gccagcccca
    23821 tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt
    23881 cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta
    23941 tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat
    24001 aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggaaga atgctagcaa
    24061 aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat
    24121 ggaagcgagc agtgccgcag caagggtaat cagcacaaac gcctgacggg aagtaaaccc
    24181 ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc tcatgccttt
    24241 acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa
    24301 agcggtaacc gggctgatgg gatgggtttt gccctgggtc gtttcgagca ggatccagat
    24361 aacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt agcggcgacc
    24421 caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca
    24481 gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac
    24541 gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc
    24601 cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga
    24661 gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca taacaatgcc
    24721 aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc
    24781 gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg gaatatagta
    24841 atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg
    24901 tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga ctttttttgc
    24961 caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt
    25021 actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa
    25081 ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagtc ctgtgcaaaa
    25141 attaaccatt tgatatgcaa attgcataac acgccagcaa attccaggaa ttttcctaac
    25201 atcgccagcg cggcattaag attacagata taaaagcaaa acgccacgta aacacgtggc
    25261 gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc
    25321 ttggtcatgg ccagtttatt aatgaggaat tccattgcat cgatttcgcc catcgggtga
    25381 atgattttgc gcaggatcca cattttctgc agttcttcct gagtagtgag cagttcttct
    25441 ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc agcgatctta
    25501 cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtagat aacttcgtcc
    25561 attttagaac cggtatcgat aagcgccgtc gcgataatag tcaggctgcc gccctcttcc
    25621 acgttacgcg ccgcaccaaa gaagcgtttc ggacgatgca gggcgttggc atccacacca
    25681 ccggtcaaca ctttacctga cgccggaaca acggtgttgt aagcgcgcgc cagacgagtg
    25741 atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt ggccttctcg
    25801 atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt agaagcaaca
    25861 acttcacctt ttaccagacg ctgcatctcg gttacttctt ccggacgttc gtcgatcagc
    25921 agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgggcaat gttctgcagc
    25981 agcatggttt taccggcttt cggcggtgcc acaatcagac cacgctgacc acgaccgata
    26041 ggtgatgcca gatccagtac gcgagcagtt aaatcttcag tagaaccgtt accacgttcc
    26101 atacgcagac gagaatttgc gtgcagcggg gttaagttct caaagaggat tttgttgcgg
    26161 gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa atagcgttca
    26221 ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag gttgaaacgg
    26281 cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg
    26341 gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc aaagatatct
    26401 tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt acgcatgcga
    26461 gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac cggcgtattc
    26521 tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa tctcgaactt
    26581 aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg actcatgtct
    26641 tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg attgagtgac
    26701 gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg aaattacaag
    26761 attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca tgactcacgc
    26821 cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac gccaggttag
    26881 cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt gccgccactt
    26941 caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc ggcgcagtgc
    27001 cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat tcgtcagcga
    27061 tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc cagaaatcga
    27121 cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa
    27181 taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg tttcgttgtt
    27241 ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt tcgtaaagca
    27301 atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa cagaagtttt
    27361 ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg tttcataact
    27421 gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac gtagccgggc
    27481 aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt cattatcttc
    27541 tctctcatcc tgcgattgcc gatcgcaagg tgaatcaacc gcgtgcctta attatggcac
    27601 cgacgcgtga acttgccgtg cagatccatg ccgacgcaga accgctggcg gaagctactg
    27661 gcctgaagct gggtctggct tacggtggtg atggctacga caaacagctg aaagtgctgg
    27721 aaagcggcgt tgacattctg attggcacca cgggtcgttt aattgactac gccaagcaga
    27781 accacattaa cctcggtgcc attcaggtag tggttctgga tgaagccgat cgcatgtacg
    27841 atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct gcaaaccagc
    27901 gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg gcgttcgagc
    27961 agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc caccgtatta
    28021 aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg
    28081 aagaagagtg gccagacaga gcgattattt tcgccaatac caaacaccgt tgtgaagaga
    28141 tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc gatgtcgcgc
    28201 agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg cgatctggat attctggttg
    28261 ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt gacgcacgtc tttaactacg
    28321 atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt cgcgcaggcg
    28381 caagcggtca ctctatcagc ctggcgtgtg aagagtatgc attgaatttg cctgctattg
    28441 aaacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca ttgatgaccg
    28501 atctgccaaa accgctgcgc ctcacgcgcc cacgtacagg caatggtccg cgtcgtactg
    28561 gcgctccgcg taatcgtcgt cgttcaggtt aaataaaatg tcatactcaa aatcattcaa
    28621 actgtatcaa gatggcaaga gaatgaatcc cgatgagcat actgtagtaa gtgattcagg
    28681 tgaaagaatg cagccaacac agagacagat tgaaggatga agagtatggg ttccacctcg
    28741 tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt tgtgcgcgag
    28801 gtggctggaa gcatccagac gctgacgcga ataaaacgca aagtgcgtct ggctgctggc
    28861 ctgaatagcg aaaatgccct gtctaatgaa gcaatggagc gcggttggca atgtctgcgc
    28921 ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt cgctacggcg
    28981 acattacgtc ttgccgtcaa tgcgggtgat tttattgcca atgcgcagga aatcctcggt
    29041 tgtccggtac aggtgatcag cggtgaagag gaagcacgtc tgatttatca gggcgttgct
    29101 cacacaactg gcggtgccga tcagcgcctg gtggtggata taggcggtgc cagtactgaa
    29161 ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat gggctgcgtc
    29221 acctggctgg aacgctattt tgccgatcgt aatctggggc aggaaaattt tgatgctgca
    29281 gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg aattacggta tcacggctgg
    29341 aaagtgtgcg ttggtgcttc cggcaccgtg caggcgttac aggaaatcat gatggcacag
    29401 gggatggatg aacgcattac cctggaaaag ttgcagcaat tgaaacagcg agccattcat
    29461 tgcggtcggc tggaggaact ggagattgac gggctgacgc tggaacgtgc gttagtgttc
    29521 ccgagtggtc tggcgatcct gatcgccatt tttaccgaac tgaatattca gtgtatgacc
    29581 ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggca tgttgcatct ggccgtcgag
    29641 caggatattc gcagccgtac gctgcgtaat attcagcgcc gctttatgat cgatattgat
    29701 caggcgcagc gcgtagccaa agttgcggct aacttcttcg atcaggtgga aaaggaatgg
    29761 catcttgaag caataagccg cgatttgctc atcagcgcct gtcagcttca tgaaatcggc
    29821 ctgagcgttg actttaaaca agcgccgcaa cacgctgctt atctggtgcg taatctggat
    29881 cttcccggtt ttacccccgc acagaaaaaa ttgctggcga cgctactgct caaccagact
    29941 aatccggtcg atctctcatc gctgcatcag caaaatgccg taccgccgcg cgtcgcagaa
    30001 caactctgcc gtttactccg cctggccatc atttttgcca gccgtcgccg tgacgatctc
    30061 gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct tccgcaaggt
    30121 tggctaaccc aacatccgct gggtaaagag attattgctc aggaaagcca gtggcagagc
    30181 tatgtccact ggccgctgga agtgcattaa tgagtaagtg ccggatgcga tgctgacgca
    30241 tcttttccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc agattcgcca
    30301 gatggctttg ccctttctgc atccgttctt cggcgctgac cactttgcgc tcctgttccc
    30361 aaatcagatc atcctgcggc agctccagca aaaagcggct cggctccggg cgcaccagct
    30421 cgccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg gcgcgggtaa
    30481 tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgatg ctgctctggt
    30541 gcggcaaaaa cccttcttcc ataccgacca tgtagacata aggaaactcc agcccttttg
    30601 acgcgtgcag agtcatcagt tgcacctgat ccagctcttc ttcactctca ccacgctcca
    30661 tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag cgtcatcggc tcatccagtt
    30721 cgctgccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc ttcatgcgca
    30781 tttcggcggc tttcgggctg ggcgatgttt cgtacagcca ggattcataa tccatgccgt
    30841 ggatcagatc gcgcaccgcg gcaatcggct cccgctccgc cagacgctgg atttctgcca
    30901 accagtgagt aaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc tgactcaggc
    30961 ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccac tcacccagct
    31021 ttttcagcgt cgccgggcca atctctctct tcggcgtgtt aacgatacgc agaaatgcgc
    31081 tgtcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg atttcaggac
    31141 gagagaaaaa cgacgtacca ccagaaattt tgtacgggat gcggttttgc atcaggaatt
    31201 tttcaaacac tcgcgactga tggttaccgc gataaagaat agcgtaatct ttgtactgcg
    31261 ttttattgac gaagtgatgc gcaatcagtt caccagtgac gcgctcggct tcatgttctt
    31321 cgttattcgc gcttaaaact ttaagctctg tgccgtaacc cagttcggag aacagacgct
    31381 tctcaaagac gtgcgggtta ttggcgatca ggatgttcgc cgctttcaaa atacgtccgg
    31441 aagagcggta gttctgctca agcttaatca ccttcagcgc cggaaaatcc tgactcagca
    31501 gcaccaggtt ttgtggacgt gcgccgcgcc aggagtagat cgactggtcg tcatcgccca
    31561 ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagttcatac tggctggtgt
    31621 tggtgtcctg atactcatcc accagcagat agcgaatttt gttctgccag cgctcgcgga
    31681 cttcttcatt gcgttgcagc agcaacgtcg gcaataaaat cagatcatcg aagtcgagaa
    31741 cattgcacgc tttcaggtgt gcatcataca gcccataaca atgggcaaaa atacggtccc
    31801 gctcgccaat cgcactggct gccgcctggg acggtgtttt gagatcgttc ttccagttag
    31861 agatggtcga aatcaactgt tgcaggagaa ctttgtcatc ttcaatcagc ccttcggtca
    31921 actctttgag caacgccagc tgatcggtat cgtcaaacag cgaaaagttc gccttcatcc
    31981 ccagcgccgc atactcacgc ttgatgatat ccagccccag cgtgtggaag gttgagatca
    32041 tcagcccacg cgcctctttg cgacccagcg tctgtccgac acgctctttc atctcgcgcg
    32101 ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg caaccgcgga
    32161 tcagatgggc gattttattg gtgatcacac gagttttacc ggaacccgcg cccgccagca
    32221 ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt agacgcatag
    32281 gtgtattgct caatcttcga acggggggag gattgtagca gaatcgcatt tgaatgtgac
    32341 cgcgtgtaag ccgctaaccg attggctgcg gatgtttaaa tcagagaggg aagaccgaaa
    32401 ccggagttca tcgtgaatcc attagactta gaaaatatcg ggtcgtaacg ttgtgttatt
    32461 actttttcag agtttactaa ggagaggtgt actcgtggcg attaatttca gtcctaaggt
    32521 tggtgaaata ctggattgca actttggtaa ctatcctgtt agccagaatg gacagttcag
    32581 caccacgtat tacgatggcc gcattcctcc tgaaatgata aaaaacaggt tagt
//
LOCUS       DAZPLM010000023        31873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPLM010000023 DAZPLM010000000
VERSION     DAZPLM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.023"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(153..1511)
                     /gene="plaP"
                     /locus_tag="ACHVC7_000927"
     CDS             complement(153..1511)
                     /gene="plaP"
                     /locus_tag="ACHVC7_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR2115036.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1501..1563)
                     /gene="yoeI"
                     /locus_tag="ACHVC7_000928"
     CDS             complement(1501..1563)
                     /gene="yoeI"
                     /locus_tag="ACHVC7_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR2115037.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1778..2707)
                     /locus_tag="ACHVC7_000929"
     CDS             complement(1778..2707)
                     /locus_tag="ACHVC7_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2115038.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2753..3577)
                     /locus_tag="ACHVC7_000930"
     CDS             complement(2753..3577)
                     /locus_tag="ACHVC7_000930"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2115039.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3660..3914)
                     /gene="yoeB"
                     /locus_tag="ACHVC7_000931"
     CDS             complement(3660..3914)
                     /gene="yoeB"
                     /locus_tag="ACHVC7_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR2115040.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3911..4162)
                     /gene="yefM"
                     /locus_tag="ACHVC7_000932"
     CDS             complement(3911..4162)
                     /gene="yefM"
                     /locus_tag="ACHVC7_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR2115041.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4445..4495
                     /gene="hisL"
                     /locus_tag="ACHVC7_000933"
     CDS             4445..4495
                     /gene="hisL"
                     /locus_tag="ACHVC7_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR2115042.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4471..4594
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4641..5540
                     /gene="hisG"
                     /locus_tag="ACHVC7_000934"
     CDS             4641..5540
                     /gene="hisG"
                     /locus_tag="ACHVC7_000934"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR2115043.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5546..6850
                     /gene="hisD"
                     /locus_tag="ACHVC7_000935"
     CDS             5546..6850
                     /gene="hisD"
                     /locus_tag="ACHVC7_000935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR2115044.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6847..7917
                     /gene="hisC"
                     /locus_tag="ACHVC7_000936"
     CDS             6847..7917
                     /gene="hisC"
                     /locus_tag="ACHVC7_000936"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR2115045.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7917..8984
                     /gene="hisB"
                     /locus_tag="ACHVC7_000937"
     CDS             7917..8984
                     /gene="hisB"
                     /locus_tag="ACHVC7_000937"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR2115046.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8984..9574
                     /gene="hisH"
                     /locus_tag="ACHVC7_000938"
     CDS             8984..9574
                     /gene="hisH"
                     /locus_tag="ACHVC7_000938"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR2115047.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9574..10311
                     /gene="hisA"
                     /locus_tag="ACHVC7_000939"
     CDS             9574..10311
                     /gene="hisA"
                     /locus_tag="ACHVC7_000939"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR2115048.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10293..11069
                     /gene="hisF"
                     /locus_tag="ACHVC7_000940"
     CDS             10293..11069
                     /gene="hisF"
                     /locus_tag="ACHVC7_000940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR2115049.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11063..11674
                     /gene="hisIE"
                     /locus_tag="ACHVC7_000941"
     CDS             11063..11674
                     /gene="hisIE"
                     /locus_tag="ACHVC7_000941"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR2115050.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11771..12748)
                     /gene="wzzB"
                     /locus_tag="ACHVC7_000942"
     CDS             complement(11771..12748)
                     /gene="wzzB"
                     /locus_tag="ACHVC7_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR2115051.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12894..14060)
                     /gene="ugd"
                     /locus_tag="ACHVC7_000943"
     CDS             complement(12894..14060)
                     /gene="ugd"
                     /locus_tag="ACHVC7_000943"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR2115052.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14310..15716)
                     /gene="gndA"
                     /locus_tag="ACHVC7_000944"
     CDS             complement(14310..15716)
                     /gene="gndA"
                     /locus_tag="ACHVC7_000944"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR2115053.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15880..17250)
                     /gene="cpsG"
                     /locus_tag="ACHVC7_000945"
                     /gene_synonym="manB"
     CDS             complement(15880..17250)
                     /gene="cpsG"
                     /locus_tag="ACHVC7_000945"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR2115054.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(17355..18791)
                     /gene="cpsB"
                     /locus_tag="ACHVC7_000946"
     CDS             complement(17355..18791)
                     /gene="cpsB"
                     /locus_tag="ACHVC7_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR2115055.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(18794..20017)
                     /gene="wcaI"
                     /locus_tag="ACHVC7_000947"
     CDS             complement(18794..20017)
                     /gene="wcaI"
                     /locus_tag="ACHVC7_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR2115056.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(20014..20493)
                     /gene="gmm"
                     /locus_tag="ACHVC7_000948"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(20014..20493)
                     /gene="gmm"
                     /locus_tag="ACHVC7_000948"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR2115057.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(20496..21461)
                     /gene="fcl"
                     /locus_tag="ACHVC7_000949"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(20496..21461)
                     /gene="fcl"
                     /locus_tag="ACHVC7_000949"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR2115058.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(21464..22585)
                     /gene="gmd"
                     /locus_tag="ACHVC7_000950"
     CDS             complement(21464..22585)
                     /gene="gmd"
                     /locus_tag="ACHVC7_000950"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR2115059.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(22611..23159)
                     /gene="wcaF"
                     /locus_tag="ACHVC7_000951"
     CDS             complement(22611..23159)
                     /gene="wcaF"
                     /locus_tag="ACHVC7_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR2115060.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(23175..23921)
                     /gene="wcaE"
                     /locus_tag="ACHVC7_000952"
     CDS             complement(23175..23921)
                     /gene="wcaE"
                     /locus_tag="ACHVC7_000952"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR2115061.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(23932..25149)
                     /gene="wcaD"
                     /locus_tag="ACHVC7_000953"
     CDS             complement(23932..25149)
                     /gene="wcaD"
                     /locus_tag="ACHVC7_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR2115062.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(25124..26341)
                     /gene="wcaC"
                     /locus_tag="ACHVC7_000954"
     CDS             complement(25124..26341)
                     /gene="wcaC"
                     /locus_tag="ACHVC7_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR2115063.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(26338..26826)
                     /gene="wcaB"
                     /locus_tag="ACHVC7_000955"
     CDS             complement(26338..26826)
                     /gene="wcaB"
                     /locus_tag="ACHVC7_000955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR2115064.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(26829..27668)
                     /gene="wcaA"
                     /locus_tag="ACHVC7_000956"
     CDS             complement(26829..27668)
                     /gene="wcaA"
                     /locus_tag="ACHVC7_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR2115065.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(27761..29923)
                     /gene="wzc"
                     /locus_tag="ACHVC7_000957"
     CDS             complement(27761..29923)
                     /gene="wzc"
                     /locus_tag="ACHVC7_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR2115066.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(29926..30369)
                     /gene="wzb"
                     /locus_tag="ACHVC7_000958"
     CDS             complement(29926..30369)
                     /gene="wzb"
                     /locus_tag="ACHVC7_000958"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR2115067.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(30375..31514)
                     /gene="wza"
                     /locus_tag="ACHVC7_000959"
     CDS             complement(30375..31514)
                     /gene="wza"
                     /locus_tag="ACHVC7_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR2115068.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         7714 a         8425 c         7991 g         7743 t
ORIGIN      
        1 tgaggaaatt attcatgact ggtggaatgc agaccaataa ccaaattctc taaattagaa
       61 caaatggtta ttaatgagga tttcgataaa aaaaaataaa aaaaccggag ccaaatattc
      121 attcgactcc ggttgattat tagaaaagat gattatgcaa cgtcttcgta ctgcggaact
      181 gggttacgga agctcttggt gacgcaagcc aggtaaatca gaccgatagc agcccaaatc
      241 agacccagaa ccattgagct ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca
      301 ccacacatcg gcaggaacag atactggaag tgatctttca gcgtcttatt acgcttctca
      361 cggatccaga actgcgagat gaccgacagg ttaacgaagg tgaacgccac caacgcacca
      421 aagttaatca gcgctgtagc cattaccagg tcgaagttga ttgccagcag ggcaatcgcg
      481 ccaaccagga tgatgttcat cgccggagta cgccatttcg ggtgtacata accgaagaag
      541 cttttcggga atacgccgtc acgacccatt acgtacatca gacgcgctac gcctgcatgc
      601 gccgccatac cggacgccag tacggtaatg gtggagaaga tcagcgcgcc aacctggaac
      661 gctttacccg caacgtacag catgatttca ggctgtgacg catccggatc tttaaagcga
      721 gagatatccg ggaagtacag ctgcaggaag taagttgcaa agatgaagat catgccgcca
      781 atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc
      841 gacaggttgc tgatgccgtc aaagccggta aaggaaaagc acaggattgt cgccccggta
      901 atcatcggaa taacatgcgc atcgccagac cagaacggac gagtgctcgc cagcgtacca
      961 gcgccttcac cttcaaatac gccataaaca accatgccca gaatcaccgc gatcagcact
     1021 acctgcaaca cgacgattac agtgttgaag ttcgctacgg atttcagact acgcaggtta
     1081 aaggcggtca taaaggccac cagcgccacc acaaacatcc acgatgggat ggaaggcacc
     1141 agagcttcaa aatagatttt cgccagcaga atgttgatca tcggcgcgaa cagatagtcg
     1201 agcagagaag accaacccac cataaagccg acagtcgggc taatggattt ctgggcgtaa
     1261 gtatatgcag agccagcaga aggatagcgg cgaaccagct tcccgtagct cagagccgta
     1321 aacaggatcg caatcaatgc gaacgcatag gctgtcggca catgaccatc cgtgaggcct
     1381 gaaacgatac caaaagtatc aaacagcgtc atcggctgca tataggcaag acccatcatt
     1441 acaaccggaa ctaacgtaag cgttttacgc aattccacgc gagaggtgtt tggagtaacg
     1501 ttatgcgaca tggtcattct cctttacggt gataaccgtc gcgtaagcaa aaaattgccc
     1561 catttttttg gattcctcag cgacaacaac tgtcgatttt tagtaaatat ctatccggta
     1621 cgaagcccgg cctcttggta tgaatgattg gtttgaagca aaaaataacc gacgctgatg
     1681 aaacgtcggt ttttagtcat tttttgacag cggcgcattg tgcctaaatg ggggggaaat
     1741 gacaagagaa tgagaagctt gtcataataa tttttcttta aatggctgat tttccgtcat
     1801 cagattttgc ataaacaccg gcgatcgcac tatttgctaa aatttcatcc cgccaccagc
     1861 cgctggcaag ccctgcggtc tgttcgttcc agccaatcca gacgggttca aagagagttt
     1921 gggcgaccac tttcttctcg attaacgcgc cactatcgag aaaacgttgc accagataac
     1981 ggggtaaata gccgcagccg aggccgctaa tttgcagctc cagcttggtt ttaaaatcga
     2041 aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg
     2101 cagtgtcccc caccacaatc gcccgatatc gcttaatgat acgacggttt aacggctctt
     2161 cttcctgggc taacggatga tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc
     2221 gcgagaaacc aaactcactg gaagatggtg gctcatgcat cgcgccaaca ataatatccg
     2281 ctcgcccctg agtcagggca tcccaggaac caccgagtac gccattgata aacttcaggc
     2341 gcgtcacgct atgatgttga taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa
     2401 acgtgtcgtc cacgccaatc accagctcgt tttcccagcc ttcatgcagt ttgatcgcct
     2461 gcttttccag ttctcgcacg gtatgcagaa cttcccgccc cttctctaat aacatttttc
     2521 cggtacgggt gaatttagcg cggtgaccgc tacgatcaag taattgaata ttaaggtcgc
     2581 tttccagctt gtgaacggtg taactcagag cggacggcgt cttgtagagt ttagccgacg
     2641 ccgccgcaaa actgccttct ttttctaagg catcgaggat catcaacaca tccagcagtt
     2701 gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca
     2761 gcggcattac cagcggatcg ggatactggt attcaaatcc cagttcatta caaatccgac
     2821 tgccatcaat aatcttacct ttgccgctgt ccagactatt tctgaactgc ggaggttcca
     2881 gccccagtaa acgggccatc tgcggataga aaacattacg cgcagggtga gctggcgcac
     2941 atatattata gatgtgtccg cctttaggtg cctgtaacaa cagagtgata gcgccaataa
     3001 catcttctaa atggactaaa ttaacaccat gttcaccatc aggcgcggtt tttccggcaa
     3061 agaagcgtcc gggatgacgt cccggtccga ccagccccgc aagacgcaga atatcgaccg
     3121 aagtaccggg taaattgtgc agccagtctt cgagttcttc taatactcgt ccactgttgg
     3181 tcaccggatt acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca tagacagacg
     3241 ttgagctggt aaaaataatg cgaggaatac gatgggccag cgcgctatcc actaactctt
     3301 gtaccgcttg taaatagaac tcatcgccgg ggccgctacg acgtgccgga agcgtaatga
     3361 ccagcgcatc ggcatccatc agggcatcca gatcgtcaga atcgcaaact aactcaggct
     3421 ccatgcgaag cagatagcta tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg
     3481 tggttttact cccggtgact tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc
     3541 ctaaccaccc taacccgaca attgcgacct ttttcatccg ttatctcctg atacacctta
     3601 gatctataag gctacgctag cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt
     3661 caataatgat aacgacatgc tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga
     3721 cggtgctcct ctgtaatgcg tcgggaccag aaacctgaca aattatgttt caggggttct
     3781 ggcttcccct taccttcaaa tggcgttctg cgggtatctt tgataagttc attgatcttt
     3841 ttaacaattc gcttatctgt ttcctgccag tacagataat catcccatga ttcctcagac
     3901 cagattagtt tcactcaata atgtcctttt ctgttccttt gcctgatttc aggctatcga
     3961 ttgagtccat caatctccgg gcgttagcgg gggagcgcag tagataagcc gtctcttcca
     4021 gcgagttgta ttcttcgagt gacatcagaa cacaagcctc tccattctga cgagtaataa
     4081 ggatcggggc atgatcttca acggctttca tcattgttgc cgacaaattc tgacgcgctt
     4141 cgctgtagct aattgtacgc atgtcaatct cctcttttgt acagtttatt gtacaatgat
     4201 gggcgttaat taactattta ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa
     4261 aatccaggct tcgctatttt tggtgccgtc agcttagagg acagtcctct tagccccctc
     4321 ctttccccgc tcattcatta aacaaatcca ttgccataaa atatataaaa aagcccttgc
     4381 tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat tcacagagac
     4441 ttttatgaca cgcgttcaat ttaaacacca ccatcatcac catcatcctg actagtcttt
     4501 caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg catgagaaag
     4561 cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg gttcagacag
     4621 gtttaaagag gaataacaaa atgacagaca acactcgttt acgcatagct atgcagaaat
     4681 ccggccgttt aagtgatgac tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc
     4741 ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc
     4801 gtgacgacga cattcccggt ctggtaatgg atggcgtggt agaccttggg attatcggcg
     4861 aaaacgtgct ggaagaagag ctgcttaacc gccgcgccca gggtgaagat ccgcgctact
     4921 ttaccttgcg tcgtctggat ttcggcggct gccgcctttc gctggcaacg ccggttgatg
     4981 aagcctggga cggcccgctc tccttaaacg gtaaacgtat cgccacctct tatcctcacc
     5041 tgctcaagcg ttatctcgac cagaaaggca tctcttttaa atcctgctta ctgaacggtt
     5101 ctgttgaagt cgccccgcgt gccggactgg cggatgcgat ttgcgatctg gtttccaccg
     5161 gtgccacgct ggaagcaaac ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct
     5221 gccttatcca gcgcgatggt gaaatggaag aatccaaaca gcagctgatc gacaaactgc
     5281 tgacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg atgcacgcac
     5341 cgaccgaacg tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc
     5401 tgccgctggc gggtgaccaa caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt
     5461 tctgggaaac gatggaaaaa ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga
     5521 ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact ggaatagctg
     5581 tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt ctgaaagcat
     5641 tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg
     5701 ggaatacagc gcgaagtttg ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga
     5761 gattgccgcc gctagcgaac gcctgagcga cgagctaaaa caggcgatgg cggtggcagt
     5821 aaaaaatatt gaaaccttcc acactgcgca aaaactgccg ccggtagatg tagaaacgca
     5881 gccaggcgtg cgttgccagc aggtcacgcg cccggtcgct tcagttgggt tgtatattcc
     5941 tggcggctcc gccccgctct tctcaacggt attaatgctg gcgactccgg cgcgtattgc
     6001 gggctgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga tcctttatgc
     6061 ggcgcagctg tgcggtgtga aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc
     6121 gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc
     6181 ctttgtcacc gaagcgaaac gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat
     6241 gcccgcaggc ccgtcggaag tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt
     6301 ggcttctgat ttgctttctc aggctgaaca cggcccggac tcacaggtga ttttactgac
     6361 gcctgacgct gatatggcgc atcaagttgc cgaagccgtc gaacgccagt tagcagaact
     6421 gccgcgtgcc gaaaccgccc gccaggcact gaacgccagc cgcctgatcg tgactaaaga
     6481 tttagcgcag tgcgtagaga tctccaacca gtacggcccg gagcacctga tcatccagac
     6541 ccgcaacgcc cgcgatctgg tcgatggcat caccagcgcc ggttcggtat ttcttggtga
     6601 ctggtcaccg gaatcggcag gtgattacgc ctccggcacc aaccacgttc tgccgactta
     6661 cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc gtatgaccgt
     6721 gcaggaactg tcgaaagaag gtttctccgc gctggcttcg accattgaaa cattggccgc
     6781 cgccgagcgc ctgaccgccc ataaaaatgc cgttactttg cgtgttaacg cccttaagga
     6841 gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg caacctgacg
     6901 ccgtatcagt cggcgcgtcg tctgggcggt aatggcgacg tctggctgaa cgccaacgaa
     6961 taccccactg ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta cccggaatgt
     7021 cagccgaaag cggtgattga aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg
     7081 ctggtcagtc gtggcgcgga cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg
     7141 ggtaaagacg ccatcctcta ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa
     7201 acgattggcg tcgagtgccg cacagtgccg acgcaggaca actggcaact ggacttacag
     7261 ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc caacaacccg
     7321 acagggcaac tgatcaatcc gcaggatttt cgcactctgc tggagctaac ccgcggaaaa
     7381 gccattgtgg ttgccgatga agcctatatc gagttttgcc cgcaggcatc gctggctggc
     7441 tggctgacgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg
     7501 gcgggccttc gttgcggatt tacgctggca aacgaagaag tcatcaacct gctgatgaaa
     7561 gtgatcgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc
     7621 ccgcagggaa tcgtcgctat gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac
     7681 ctgattgccg cactgaaaga gatcccctgc gtagagcagg ttttcgactc cgcaaccaac
     7741 tacattctgg cgcgctttaa agcctccagc gcagtgttta aatctttgtg ggatcagggc
     7801 attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg aattaccgtc
     7861 ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca agtttgatga
     7921 gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt
     7981 ttcaggtgga ccgttttgac aaactcgcct ttgaaccagg cgtgatcccg gaactgctga
     8041 agctgcaaaa agcgggctac aaactggtaa tgatcaccaa tcaggatggt ctgggaacac
     8101 aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag atcttcacct
     8161 cgcaaggcgt acagtttgat gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg
     8221 actgccgtaa gccgaaagta aaactggtgg aacgttatct ggctgagcaa gcgatggatc
     8281 gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg
     8341 gcattaatgg tttacgctac gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc
     8401 tcactaaacg agaccgttac gctcacgtag tgcgcaacac caaagagaca cagattgacg
     8461 ttcaggtgtg gctggatcgc gaaggtggca gcaagattaa caccggtgtt ggcttctttg
     8521 atcacatgct ggatcagatc gccacccacg gtggtttccg tatggaaatc aacgtcaaag
     8581 gcgacctcta tatcgacgat caccacaccg tcgaagatac cggcctggcg ctgggcgaag
     8641 cgttaaaaat ggctcttggc gacaaacgcg gtatttgccg ctttggtttt gtgctaccga
     8701 tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac ctggaataca
     8761 aagccgagtt tacctaccag cgcgtgggcg atctcagcac cgagatgatc gagcacttct
     8821 tccgttcgct ctcatacacc atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg
     8881 atcaccaccg tgtagagagc ctgttcaaag cctttggtcg caccctgcgc caagccatcc
     8941 gcgtggaagg cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct
     9001 tgataccggc tgcgccaatc tgaactcggt gaagtcagcc attgcgcgcc acggttatga
     9061 accaaaagtc agccgtgacc cggacgtggt gttgctggcc gataaactat ttttacccgg
     9121 cgttggcact gcgcaagcag cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat
     9181 caaagcctgt acccaaccgg tactgggcat ctgcttaggg atgcaactgc tgggtcggcg
     9241 cagcgaagag agcaacggcg tcgacttgct gggcatcatt gacgaagacg tgccgaaaat
     9301 gaccgacttt ggtctgccgc tgccgcatat gggctggaat cgcgtttacc cgcaggcagg
     9361 caaccgcctg tttcagggga ttgaagacgg cgcgtacttt tactttgttc acagttacgc
     9421 aatgccggtc aatccgtgga ccattgccca atgtaattac ggcgaaccgt tcaccgcggc
     9481 ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg gtgccgctgg
     9541 cgctaagttg ctgaaaaact tcctggagat gtgatgatta ttccggcatt agatttaatc
     9601 gacggcaccg tggtgcgtct ccatcagggc gattacggca aacaacgcga ttacggtaac
     9661 gacccgctgc cgcgcttaca ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg
     9721 gatctgaccg gggcaaaaga tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc
     9781 gcgggcgtta acgttccggt gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg
     9841 gcgttactgg aagcgggcgt tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca
     9901 gaaagggtga aaggctggtt tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat
     9961 gtccgtattg acgagcaagg caacaagcag gtggcggtca gcggctggca agagaactcg
    10021 ggcgtttcac tggaacaact ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg
    10081 tgtaccgata tctcgcgcga cggcacgctg gcaggctcta acgtctcttt atatgaagaa
    10141 gtgtgcgcca gatacccgca ggtggcattt cagtcctccg gcggtattgg cgacattaac
    10201 gatgttgctg ccctgcgagg cactggcgtg cgcggcgtaa tagttggtcg tgcattactg
    10261 gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata atcccatgtc
    10321 tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg
    10381 gcgatattgt accgctggca aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct
    10441 acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta tctcgcgtgg
    10501 cggaagtgat cgacattccg ttctgcgtgg cgggtgggat taagtctctg gaagatgccg
    10561 cgaaaattct ttcctttggc gcggataaaa tttccatcaa ctcccctgcg ctggcagacc
    10621 caaccttaat tactcgcctg gcggatcgct ttggcgtgca atgcattgtg gtcggcattg
    10681 atacctggta cgacggcgaa accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa
    10741 gccgcacccg cgtcactcag tgggaaacac tcgactgggt acaggaagtg caaaaacgtg
    10801 gagccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac ggttacgatc
    10861 tcgaacaact gaaaaaagtg cgtgaagttt gccacgtccc gctaattgcc tccggtggcg
    10921 cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg
    10981 ctgcttccgt attccacaaa caaataatca atattggtga attaaaagcg tacctggcaa
    11041 cacagggcgt ggagatcagg atatgttaac agaacaacaa cgccgcgaac tggactggga
    11101 aaaaaccgac ggactaatgc cggtaattgt gcaacacgcg gtatccggcg aagtgttaat
    11161 gctgggctat atgaacccgg aagccttaga caaaaccatc gaaagcggca aagtcacctt
    11221 cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca atttcctcaa
    11281 cgtagtaaat attgcgccgg actgcgacaa cgacacgcta ctggtactgg cgaatcctat
    11341 cggaccaacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc accaatggct
    11401 gttcctgtat caactggaac aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc
    11461 ctataccgcc aaactgtatg ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga
    11521 aggcgtagaa accgcactgg cagcgacggt acatgaccgc tttgagctga ccaacgaggc
    11581 atcggacttg atgtatcacc tgctggtgct gttgcaggat caggatctgg atttaacgac
    11641 ggtaattgag aacctgcgta aacggcatca gtgagttgca gggtaagcgg atgcgatgtt
    11701 gttgtcgcat ccggcaaaaa aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa
    11761 gctgcaaaga ttacttcgcg ttataattac gcagagcgtt acgccccagc acaatccccg
    11821 cgccaaccat gccgcccagc agcaccgcca gaatcaaggt aattgccttt ttcgggctat
    11881 cgcgacgaat aggtaacgtc ggtttcatca cataacggta agcatgaata tcaagatcat
    11941 caacctttaa gttttcaata tccaacaggt tttgacgtgt ctgatagtag tttggtgaga
    12001 acaccaacgg acgggtcgcc tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa
    12061 ggaacaacgt atcttgtgtc acatcttcag tctgctgaac ctgtggcttt gccacctgcg
    12121 cctgattcgc atactgcaac gcttcctgaa tctgacggat acgcagatct ttctgctcct
    12181 gcgcgactac ttcctgcgtt cttaaagagt cctgcaagtt tttccgtccc agagcaatgt
    12241 tgtctttgag atccttttct agctcttgat tcactttatc atcaacttgc tgaatgtatt
    12301 gggccaactt catttgtgct ccctctgcag tttgcccaac ataagaaaca gtcaatggta
    12361 attgctggtt cttaacagaa ggttcgatgg taagtttttc tggttcttcc tgattatcca
    12421 gcgtttctgc taatgcagag aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat
    12481 ccgatacttt cggtgcagcc tgaccataga taacattcat ggcattgtta tagccagcaa
    12541 tttgccccac gtcgggctga gtgataattg ctgttgacgt ccatttctcc ttcgctactg
    12601 ccaaatatcc aatagccagg gcaatagcca caatgacgga aatgatgatt gtcatcttgc
    12661 cacgccacaa ctgcactagt aaatcaatca aatcaatctg ttccgggtca tggttttgcc
    12721 cagaaacatt attattttct actctcatca ttaccctaac tgacggaaat ttccaagtcc
    12781 gcatagttta gcgataatcc tagtgaatgc tataggataa gtgataaaaa taatgaatta
    12841 ttggggaggt aacaaaagac aatcagggcg taaatagccc tgataacaag atgttagtcg
    12901 ctgccaaaga gatcgcgggt gtatacctta tctgccacat ctttaagctc ttctgccatt
    12961 cggttagaga taatgacgtc ggcttgttgt ttgaagatgg cgagatcacg ttccagccga
    13021 gagttgaaga atgagtcttc tttcatcact ggctcgtaga tgatcacttc aacgcctttc
    13081 gccttgatac gcttcataat cccctgaata gaagacgcac ggaagttatc tgaaccgctc
    13141 ttcataatca gacgataaat acccaccact tgcggtttgc gtgacaaaat ggcatcggca
    13201 ataaaatcct tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca
    13261 gactgatagt tcgccagtaa ctgcttggta tctttcggca agcaataacc accataacca
    13321 aacgacgggt tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc
    13381 tggcgagtat tcagacctaa actttctgcg tagctatcca gttcgttaaa gtatgccacg
    13441 cgcatcgcta ggtaggtgtt tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg
    13501 aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct
    13561 gcgcgttctg aacgctcacc gatgacaata cgtgaaggat ggagattatc gtaaagggct
    13621 ttaccttcac ggagaaattc cggagagaaa ataatatttt cagtacgata tttcttatgc
    13681 atcgcttcgg taaaaccaac gggaaccgtt gatttgatga ccataaccgc ataaggattt
    13741 atctcaacta cgtctttaat tactgattct acactggatg tattgaaata attagtttta
    13801 ggatcatagt cggttggagt ggcgatgatg acataatcag catcccggta ggcttcattt
    13861 ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta
    13921 tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat
    13981 gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac atagccagta
    14041 ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt
    14101 ttttagcatc ctgataagag ctaaatgttt taacgctacg ttattagctg gtcaacgcta
    14161 cgaaaaacaa ttcagatttt ctttataaga atagctgata tttattaagt taatattaag
    14221 cagtgaaatt tagtctataa gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg
    14281 gcctcaattt tactgttggt taaatcagat taatccagcc attcagtatg gaacacacct
    14341 tctttatcaa tgcgcttata agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg
    14401 ttcgcaggca gaacagcggc acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc
    14461 ggaaccggga taccgttctg tactgcataa gcaacgacat cacgcagcgc ctgctggtag
    14521 tcatcggcaa tttgcttgaa gtacggagcc agcagcaggt tagcgatctg cggattttcg
    14581 gcatatgcat cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa
    14641 atcttcgcga tttcgccgta gttcagatcc cagttgtatt cttcagacgc agcacgcagc
    14701 tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcacg gcgaactttt
    14761 tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag aactttagat
    14821 gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc
    14881 agcgacagcg gttctccgag atccagcgcg ctctggctgg tccatttacc ggtacctttg
    14941 ttagccgctt catccaggat cacatcaacc aggtagttac cgtcttcatc ttttttagtg
    15001 aagatatctt tggtgatgtc gatcaggtag ctgctcagtt caccgttatt ccactcggta
    15061 aaggtctgtg ccagttcttc gttagagaga ttcaggccgc ctttcaggag agaataggct
    15121 tcagcaatca gttgcatatc accgtattca ataccgttgt gaaccatctt cacataatgg
    15181 cctgcgccat cagcaccgat ataggtaacg cacggttcgc catcttcagc aacggcggcg
    15241 attttggtca ggatcggcgc aaccagttca taggcttctt tctggccacc aggcatgatg
    15301 gacggccctt tcagcgcccc ctcttctccg ccggaaacac cggtaccgat gaagttaaag
    15361 ccttcggcag aaagctcacg gttacgacga atggtgtcct ggaagaaggt gttaccacca
    15421 tcgatgatga tgtcgccttt atcgagatat ggtttgagcg aatcaatagc agcatccgtg
    15481 cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac
    15541 tctttcaccg tatagtaagg aaccagtttc ttgcctggat tttcggcaat cacttcttcc
    15601 gtcttttcac gggaacggtt gaaaatagag acggtataac cacggctttc gatgttgagc
    15661 gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt tgacattgtt
    15721 tactcctgtc agggggtggt caccgccgag tgtgcggctt aaatgtggta tagatgttaa
    15781 ctcactcata gagatggtga atagcattac ctttgctata ccacactgaa tatctcacct
    15841 tttccgtctt ttaaaactta tttatatgaa attactgaat tacttgttca gtaactcaag
    15901 aataagtttt gtcttttctt ccatcagcgg cacatcaccg cgagattcca cattcaaccg
    15961 caccaccggt tcggtgttgg aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct
    16021 gatgccatcg gtgcgatcca ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt
    16081 aatcgcctca acgggttgcg ccagtttgct gttgatctca ccgcttgccg gaaacgctgc
    16141 catccggtcg cgcaccagtt cgcccagcgt ttttcctttc aggcacacca gttcggcgac
    16201 cagcagccac gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc
    16261 gctcatttca ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg
    16321 tccggttttc gacatcaccg gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca
    16381 ggagagacgc ggatcgtgga tgatcttcgc gccgggattt ttttcgagga atgcttctgc
    16441 cagcaggccg acaatgtagt agccctcaat aaactgccct ttttcgtcaa acaggaagca
    16501 gcggtcaaaa tcgccatcaa aagcaatgcc catatccgca ccgtgtttga tgaccgcatt
    16561 gcgggtgtcg tcgcggcatt ccggcagtag cgggttagga ataccgttgg ggaaattgcc
    16621 gtccggcgtg ttgtgcactt tgattaattc cacgggtgcg ccaagggctt taaagcgggc
    16681 ttcgatggcg tccaccaccg gacccgctgc gccattcccg gagttaatca ccagcttgag
    16741 cggcgtgagg tttttgacat tgatataacc gaacaggtga tcaacgtaag cgtcacgcag
    16801 attgatttgc tgatagcgac cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc
    16861 tgccagacgc tggacatcgc gcagtccggt atcaccgctg atcgggcgag ccccttcgcg
    16921 caccagtttc atgccgttgt aatccatcgg gttatggctg gcggtaactt caatgccgcc
    16981 atccacgccg agatggaacg tggcgaaata gatctcttcg gtgccggaca tgccaatatc
    17041 cagcacatcg acgcccgcat cctgtaaacc tctcgccagc gccagtttta aggtttcgct
    17101 ggtgaggcgg acatcaccgc ctaacacaat ggttttcggt ttgagaaatt cgccataggc
    17161 gcgaccaatg cgccaggcga tatcttcatt cagttcttcg cctaatttcc cgcgaatatc
    17221 ataggcttta aagcaggtta attttttcat gtcgttaccc tttttcaggc aatagttggc
    17281 cctgaccgaa acggccaatt ttgtttttgt cgttatttac atctcggttt cgcattcatt
    17341 gcctgatgcg acgtttacac ccgtccgtag cgatccgcga aacgcaccac atcatcctct
    17401 tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc
    17461 aggcaatgca tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata
    17521 tcaccatcaa tggtgacttt tgccgttccc gcgacaacca cccagtgttc cgcgcggtga
    17581 tggtgcatct gtaccgacaa gccctcgccc ggtttcacgg tgatgcgttt cacctggtag
    17641 cggtcgcccg cgtcgataga gtcatatttg ccccacgggc gatagacttc gcgatgcacc
    17701 cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgcacc
    17761 gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc
    17821 ccgacggtgg ttacgaggcc agattcggcg tacacatagc tgttttcagt tttgtgatta
    17881 atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctaatttc ccataatgag
    17941 gaccaggagc cgacatcgct ccagcccgca tccatcggca ccacaacggc atctgccgta
    18001 cgttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc
    18061 acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga
    18121 atatccggac gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg
    18181 ccgctgttcc agtaatattc gccgcttgcc acataggcct gggcggtttc cagattcggt
    18241 ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccgc agacacttca
    18301 ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc gaaggtcacc
    18361 agcttgcccg cttcggcata tggcatggca ttacgcacgg cggcacggaa cgcgtcttca
    18421 tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga
    18481 cgttttgccg ccagcgccgc cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg
    18541 agaataatgt tctcggtaag tttgttcagt tgacgcagct gttccgcgac aataaagcgg
    18601 tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg
    18661 gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg
    18721 cgggaaagcg gccataagcg gctaccggag ccacctgcca tcacaactgg atagagtttc
    18781 gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat
    18841 cgagtgtgcg ttcggcatat tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct
    18901 gacggatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatgcccg
    18961 gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac
    19021 cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt
    19081 tttgcaccac cagatggcaa tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt
    19141 gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg
    19201 ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg
    19261 cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga
    19321 tttttttgtt atccggcagg tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa
    19381 catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgttt tccgccgcca
    19441 cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt
    19501 tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgctg cctttgcctt
    19561 ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg
    19621 tacgcgcacc agaaagtttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca
    19681 ccacgccgat aatgcgatcc ggcttccagc gacgttgcgc catcagagga aagaaactgc
    19741 tgacggcaaa actgccgaga tgtaacaggc gtttcagggt gctcggctgt tttggcacgt
    19801 acagcgggca gcgccacacc gtggcggccc cctcttctcg tttgtaccgc caggcggaat
    19861 agttctcgcc cacctgccac tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat
    19921 gaccttgtgc cgccagccat tccaccatct cgccggtgta tttgccgata ccggttaact
    19981 ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt
    20041 ctcagcgaga aaataggcgc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc
    20101 cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtatcagct cttcttcagc
    20161 gactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt
    20221 atcgtcataa aagtgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag
    20281 ccccagttcc gccatcgtca gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac
    20341 gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag
    20401 aaactcaccg cgactgttct cgacaataaa gtcgagagag acaagcgggg tggagcgcac
    20461 taccgtggca aagtcttcct gacgtaaaaa catcattacc cccgaaagcg gtcttgattc
    20521 tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag
    20581 ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg
    20641 gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg
    20701 cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc
    20761 tccagccaga cttcgtgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg
    20821 acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggtgcattc
    20881 tgtgccgtcg cctcgtggaa gcgacgcagc aatgcaggga tcacatgcga attactcggg
    20941 tggaagttgt catgcgggcc atacaggttg gtcggcatga ctgagcggta atcgcgtccg
    21001 tactggcggt tgtatgattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc
    21061 tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc
    21121 agtttcgggt agatacagga cgacccaaga aacagcagtt tgttcacgtc gttctgatgc
    21181 gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag
    21241 gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt
    21301 tcgctggcaa agaaatcatg caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg
    21361 cgtaatacca gttccacatc accgcgctgt tcgagctgcc gcctgatggc agaaccgacc
    21421 atcccacgat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga
    21481 tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat
    21541 tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt
    21601 cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc agccggacgg aagtaacgcg
    21661 gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg
    21721 aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct
    21781 gtgccgctgc catttccacg aactgacgca cggagtactg aacgccggtc gcgataacga
    21841 aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt
    21901 ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg
    21961 cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg
    22021 attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa
    22081 cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg
    22141 tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat
    22201 agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac
    22261 ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca
    22321 ttgcgcccag gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag
    22381 aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct
    22441 gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca
    22501 cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac
    22561 cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt
    22621 tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc
    22681 gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg
    22741 gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta
    22801 atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat
    22861 atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgccg
    22921 acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac tgacggacga
    22981 ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga
    23041 tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact
    23101 ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt
    23161 ggttttcctt atattcagac tttgttatat aaggctttcg tctttgaggt agtacgttgg
    23221 cgtaaatgcc aggataattc agcccaaaag ccaggcacat gtaatatttg tcgttggact
    23281 tttttcgcgt cagcacacaa ttccatatta ttggtggtag ataccccacc catggaaaat
    23341 tcagacacca ggccattgag ttttttaaat gcataaccgg ctttatacat tttggctgcc
    23401 agcgcgtagt cggaagaaac tttatattcc aggtcataac gccatttttt caagccggat
    23461 acagggaaaa atatcgcctg atgactggcg ggcaggctgt gataaatata ccagcccggt
    23521 ttggcgctac gtttaatttt atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc
    23581 atcatgttat ctttttgcat ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa
    23641 atatcgcccg aattgagaaa caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc
    23701 atggcgtcgt agataccgtt atctggctcg ctgacaaagc gtaggttata gataccattg
    23761 agattttcca gatactcacg ggtgccgtca ttggaaccgc catcgacaac aatccattcg
    23821 aagctgatat cttccgcctg cgccagatgc gccagcgagg catgtgtttt gactatccct
    23881 tcgaggttac gaaacgcgac agtgattatg ctaagcaaca tgttcttatt cttacctcgt
    23941 aatatttaac gcttttcgca aaataaatgg acagacgatt aaaaatgcat attccgggct
    24001 aaatatcgaa ccggtaaaaa acagcgatac cggagtaaaa agatataatt gcacgcgaaa
    24061 attgcggtta tcaccaaaag cgttgatcat catttttatc actttcccca tgtaccacag
    24121 cgataaaaac actgcgaacc aggaaaaata aataatcagc agatacaaac cattgtctat
    24181 ggtttttccg acatccgcac cgttaaatat tccgaatgat gcgacgtatt cataaagtga
    24241 gccaaatctg actacaccgt caatatgggt caaagaataa ccgaccatca ccagcggacc
    24301 gacaatacga taatacgatg acgatccttc cgtacctaaa tcgcccagac gggtggaaat
    24361 ataaggaaac gcgattacca caccaaccag gaatacagcc agagaaatca atgccaacgg
    24421 taactttttc ttaatcgcct ctttattcag atattgaaat gcccactcca gcaaataaaa
    24481 caggataaaa gtcataaccc ctgaaaacga tccggataat attatccctg cgagaatcat
    24541 agcatcggtt ttaggcgttt tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa
    24601 tgccagagcg aaaaatgccg gttcgaaata aagtgctgtc gtgcgcttgc cgccgaattt
    24661 aatgaaattc agtacatagc tgttgctgta aatcagatat ttcgaaatcg actccataat
    24721 actgctgcca ccggtgagga taatttgcgc catctccacc gccgccagcg ccaccaccag
    24781 cccgatcacc agataaaaga aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt
    24841 aaagcgaata ctccacacca tgccaataat gatcacaata tagacaaaca gcatggttga
    24901 agtgacgtat ttactggcat ccagcgactg accaaacaga tagttaaacg ccgtgagtcc
    24961 cgcaccaatc cctaaggcaa tcatcaattt tttaacgctg atgcgttcta aaaacagcag
    25021 taacaagaca ggtaaaaaag tgacgatggt gatggggaaa ctttcgccaa gctgggcgat
    25081 tttgacgtta accagcaggt agatcagcgg cagcagcagg tagctacaga ttctgataga
    25141 agttgacata ctcctccagc atctgttgtc cactgtaggt ggcgcggctg cgttggctga
    25201 actcagccag cgtggtacca aatatcgcct gcgcgatttc cggtttgctt aactgcacca
    25261 gttgcagcac gtcttcttcg tcgacggttt taccgccgga tttttgcaac acttcccgtg
    25321 ccgcgtcgct atgggtggca atcaccggca cgccaattga taacgcctca cacaaaatca
    25381 gcgggtagtt atcgacgcga gaactgaata ccagcgcatc catctgattg agcgcgctca
    25441 tcaacttgcg cttgtcagtt tcaaagccgt gattaaccac gttgccagcg gtgaacggcg
    25501 agaacttacc aaaggtatgc agttcgattt tgtcgcccag cgccatcatc tcacgcacca
    25561 gttgctggtt agtcttgccg tcgtaacgca agtcatgcgc caccaccgcg attttcggct
    25621 tgccctgggt ttcgcgcacc ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa
    25681 taccattatt gataatccgg caacgccctg gaccgtacag gctattgaaa gcgtcagcca
    25741 catgctggct gggggaaata aactgacagc ccagcgccag catctcacgg aataactggc
    25801 gtttgcccgc caccagctgg tgtgcgcgat caatcttcac cggcggatag ttatttaagg
    25861 tcgggcattt ctggcagccc gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc
    25921 cggtaacact ccagtggtcg tgcagcgtcc agaccagagt gacgtccggt ttatggtttt
    25981 tcactttttc gcaaaagcgc accacgctct taagatttag ccagtagctg tgcagcacat
    26041 gaaaatgcag gaccaccgga cccggtgtgc gagtaatggt gcgatataac tcattgaaat
    26101 tgccaaacag atcgcgatta aacagacgaa acagggcaat attcgccatc gcggtcatcc
    26161 gcggcgtatg tttgatgacc tgcggatagt tctgatggct gacgctctct ttgccgcctt
    26221 tgccgtaacc gtagacaaaa tgtgacgcca gcccctgttg cagcgcacgc tggtggagat
    26281 ctaacgccac acctgctgcc ccgccttccg ccagtcgcac attaaattgc agaatattca
    26341 tttaattacc ttcactcgcg ctttttctcc caccaccagc gcgttgtccg ggacagagtc
    26401 gagcaccacg ctgcccgcac ccacggtgac gttgttacca agcgtgatat caccaagaat
    26461 aatgacgttg gcaccgagtt cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc
    26521 accacgattg ccgatagtga cgccgtggcg aatggtgaaa tcatcccccg ctaccacgtt
    26581 tttattgatc actacggcgt aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc
    26641 ggcctggatt tcataaccga aaaagcattc ggtgataatg cgatacagca ccagcagcgg
    26701 ggccgcccac agattgttga ggacgttctt tttgcgccac accgaacaaa aatgagcaac
    26761 acgataggca agaaccatgc agcacgggcg taaactccag ctgttggcac gcagatcttc
    26821 cagcatcttt agcgcccccg aataccatca gccagacgtt tgccgttacg caccgacagc
    26881 agcgtcagca aagtgcgcca ggtcatacgt ttattgcgaa tctggtagag ggtaaacagc
    26941 tgatattttt tgcttgcacg gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac
    27001 ccggagaatt ttttcggtga cgaggtgatc tgcatttcac cgtgattgat atgcaggatc
    27061 tgcgtcgctt cttccacttt ccacggttcg ccgtactcca ccaccatccg caggaagatg
    27121 tcgtaatcct gtgctgcttt cagttcggta tcgaacaaac actctttgaa acgccatgcc
    27181 caagtaaaga cctgattgcc aatgatattg cgtttataga acaggcggcg tgagtacggc
    27241 gatttgggat acagtggtaa gcttgccggt tgggaataaa cttctccctg gcatacgtag
    27301 tcgttagcgt acaaaaaggc gtgagtgacc agttgctgtt tatgggcgag gaagacgctc
    27361 agacggttgg gtgtccattc gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc
    27421 agcataatcg cctggttacg taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag
    27481 gtgatacgcg gatcgttgag ggcagtgacg tactgttgca gttgttccca ggaggtggag
    27541 caatcatcca cgatgatcat ctcccagttg ctgtagtcct ggcgcaggac cgattttatc
    27601 gcccgaatcg ccagttgttg gcggttccag gtcggcatat agattgaaat cagcggattc
    27661 tctttcatag gttgttctcc cggcttatga gctggtttgc ttgccggatg cggcgtgagc
    27721 gccttatccg gcctacgggg cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat
    27781 attcgtattc gtaatagcca taatcctgat acgcgctggc gcggcggaag atggagttca
    27841 gaatcacccc tttcaccgga ataccgtttt gctcaaagcg gctcagactg gtttccactt
    27901 ctttcaatgt gttgaccgca tagcgcgcca ccattaacgt ggttccagcg tgacgcccga
    27961 caattgctgc atcggtcact gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt
    28021 ttttactcgc ccagctcacc aattcggcaa agcgttcgct catcaacagt tcagaaggat
    28081 ttggcggcac ctgaccgcgc gggatcaggt caaatttggc aatagaggtc ggtttagctg
    28141 cggtagtaat atcgccctgg ccaatcagaa tttccgacag gccattgacg ttattggtac
    28201 ccaacagctc gtgggtgtag cctttgcgca tatcgcagtc gatcaacaac acccgtttat
    28261 tggtctgact gatcaccgcc gccaggttgg cgcagacaaa ggttttaccg attgacgggc
    28321 tcacccctgt catcatcaac acattgttct gcgcctgcat catcgcgaag tgcagactgg
    28381 tacgcagact gcggatggct tcaatcgcca gatcggttgg attccccacc gccagtagct
    28441 ggctctgttt atagcgttta atccctttga tggttttgac gctatcgcgc gctttctgcc
    28501 attccgacag cgggatgctg gcatagacgc tgataccatg ttcttccagc acctgcgggc
    28561 tttcgatgcc gcgattaaac aacgagcgca gcagcacacc cacgatagag agcatcaggc
    28621 cgaggataat tgcgccgagg ataatcagtc ctttcttcgg tttcagcacg ccaggctggg
    28681 tgattgccgg gtcaacaatg cgcacatcgc cgacggtgct ggcttcggtg attttcagct
    28741 cctgctcttt attcagcagt tgcatataga cctgctgacc agactcgaca tcgcgggtca
    28801 ggcgcacaat ctcctgctgg gttttcggca tcgccgttac gcgaccgtta agtttggctt
    28861 tctcttcttc cagcgcctga cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg
    28921 tgtacagctt ggagatttcc gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt
    28981 tcaccatcga atcgagcacc gctttcgctt ccagcggcag atcaacagaa tctttatcct
    29041 gacggaaggc attcagtttg ttttcggcaa catcgaggcg gctacgtact tccggtaact
    29101 gttgcgcgag gaaagcgagg cttttcgacg cttccgccga tttgcgctca atattttgtt
    29161 cctgatagtt acgggcgatg ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg
    29221 tataagtcag gctcagtacg cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt
    29281 gcagttggtt gatcatcccc aacgtggagt atttggtgac ggtaaactca ctgcccgggc
    29341 gggcgtgaat ggcttcaacc atcagcgtga cgccttcttt tttcagcatc tggcccgctt
    29401 gcccacgggc gctaaagccg ccatcactgc tcagggtgta gtttttattg tccagcacat
    29461 taagcgtaaa cacctggccc gccatctctt tcgggcggtt aaaggtagtc actttcaccg
    29521 tctcgttctg gcgtcccatc agtcgctccc agcccgcccc gaaaatcggg aacgtgtttt
    29581 tgctcactgc aatatcgagg tcgagatcgt ccaccgtttt accaagcacc agacgcgaac
    29641 gaatcaactg gatctcggcg tccgatgcag gcggtttgtt ggctaatgcc gaaccaatgt
    29701 cctgcactaa cgaattgccg ctgttttgct cgatttgtac cagtgcgtcg gcactataaa
    29761 tcggcgttgc gaagaaggtg taaaccacgg cacagagggc aaatacagcg gtgatgccaa
    29821 tcacccacca gcgcgcttca atgacggtgc cgaccaggcg accaatatcg atttcatcac
    29881 tgcccgttac cggagcggca tgttgtttta ctttttctgt cattcttata cctgctctgc
    29941 gttcaatgcc tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa
    30001 cgtttcccgg cttttgcgat acggatcggg gatttcacat tcgttatccc agtgaccaaa
    30061 cagcatcact ttgccgcgca tctcaggtgc catctcgcat aagcgttcga tatggcgctt
    30121 ttccatggtc aaaatcaggt catagttgcg acacaggcgg cggctgattt gtcgtgcaca
    30181 gtggccttcc agagacagtt gatgttctgc ggcgacgctg atagcggtag gatcagcgcc
    30241 cttaccgacc agcgcgccga gtccagcgga ctccactttc agttccgggt gataacgttg
    30301 cagtaagcgt tccgccgtcg gggaacggca aatattgccg acacagacaa ctaatatgtt
    30361 gttaaacatg acgattacca gttatgaatg tcgctggctg tatccgtcat gtaacggaca
    30421 ccgctaatag ttggcaacaa ctgattgatc agacggttcc agcgggaaac cggggcggtg
    30481 gtgacataca ccacgtcata aggttgcagg cggaattctg tcgccatcac cagcgacgtg
    30541 gcatcggaca tatccagctg gtagatattg gcaatcttgc cattacgccc gccctcgcct
    30601 ttcagcggac gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc gataccttca
    30661 gcattaccca gggcttcagt cagggtcatg ccgctaaagt ccattttcag ggtgctctgt
    30721 ttcttcactt cacccatcac aaatactttc agatcatcat tacgcggcac gtagagaata
    30781 tcgccgggat aaagcagtcg gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa
    30841 gaaatgcgtt cttcacgacc attgtgtgtt agcactacgt tgcgccagtc agcggtgtcg
    30901 gtcaggccac ctgcggcgtt gatggcgtcg agaatagtca gtggcacgtt ggtgatcgcc
    30961 tgttgaccgg atttattcac ctgaccggag atataggcct tttgtgagcg gaaggcggcg
    31021 atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt aatatcactg
    31081 cggatttcag cgagcgtttt cccgactacg tgaaccttgc cgatatacgg gtaaaacata
    31141 gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg accggctggc
    31201 gtggtgagtt ccgggtgatc ccagacggtg acattcagaa cgtcgcccgg cccgacgcga
    31261 tactggtaat tcgcgatctc gctttccagc gtcatattgg ggcgcgctac attcgggcgt
    31321 gggcgtaatt ggtcaatcag gcgcggggtc agcggataaa cattcaccat tttgtcgaga
    31381 tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat attgctgccc
    31441 ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat caatttcatt
    31501 ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt gcactattaa
    31561 tagcaatatc gccatgcaca tttaccttgc agttaattga ataaaaattt aactggcatc
    31621 agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag actggtcttt
    31681 caggcatcca gacacgctac cgcccctggc tttttagcca ccaatacact gatttagctt
    31741 aatttttcaa accctctctg catacaatcg ttgatgagaa agggttattg cggaaattaa
    31801 cttccgaata taaggtgaca ttatggtaat tgaatattgg ctctccaata atgcaggagg
    31861 aagtgttaca gct
//
LOCUS       DAZPLM010000024        31224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPLM010000024 DAZPLM010000000
VERSION     DAZPLM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.024"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHVC7_000960"
     CDS             complement(<1..559)
                     /locus_tag="ACHVC7_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR2115069.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHVC7_000961"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHVC7_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR2115070.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHVC7_000962"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHVC7_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR2115071.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHVC7_000963"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHVC7_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR2115072.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHVC7_000964"
     CDS             complement(4001..6322)
                     /locus_tag="ACHVC7_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR2115073.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHVC7_000965"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHVC7_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR2115074.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHVC7_000966"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHVC7_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR2115075.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHVC7_000967"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHVC7_000967"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR2115076.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHVC7_000968"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHVC7_000968"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR2115077.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHVC7_000969"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHVC7_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHVC7_000970"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHVC7_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR2115078.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHVC7_000971"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHVC7_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR2115079.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHVC7_000972"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHVC7_000972"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR2115080.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHVC7_000973"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHVC7_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR2115081.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHVC7_000974"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHVC7_000974"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR2115082.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHVC7_000975"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHVC7_000975"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR2115083.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHVC7_000976"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHVC7_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR2115084.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHVC7_000977"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHVC7_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR2115085.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHVC7_000978"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHVC7_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR2115086.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHVC7_000979"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHVC7_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR2115087.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHVC7_000980"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHVC7_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR2115088.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHVC7_000981"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHVC7_000981"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR2115089.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHVC7_000982"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHVC7_000982"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR2115090.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHVC7_000983"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHVC7_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR2115091.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHVC7_000984"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHVC7_000984"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR2115092.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHVC7_000985"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHVC7_000985"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR2115093.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHVC7_000986"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHVC7_000986"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR2115094.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHVC7_000987"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHVC7_000987"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR2115095.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         7309 a         7970 c         8448 g         7497 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttg
//
LOCUS       DAZPLM010000025        30986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPLM010000025 DAZPLM010000000
VERSION     DAZPLM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.025"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHVC7_000988"
     CDS             59..1423
                     /locus_tag="ACHVC7_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115096.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHVC7_000989"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHVC7_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR2115097.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHVC7_000990"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHVC7_000990"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR2115098.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHVC7_000991"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHVC7_000991"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR2115099.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHVC7_000992"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHVC7_000992"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR2115100.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHVC7_000993"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHVC7_000993"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR2115101.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHVC7_000994"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHVC7_000994"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR2115102.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHVC7_000995"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHVC7_000995"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR2115103.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHVC7_000996"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHVC7_000996"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR2115104.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHVC7_000997"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHVC7_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR2115105.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHVC7_000998"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHVC7_000998"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR2115106.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHVC7_000999"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHVC7_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR2115107.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHVC7_001000"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHVC7_001000"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR2115108.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHVC7_001001"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHVC7_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR2115109.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHVC7_001002"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHVC7_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR2115110.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHVC7_001003"
     CDS             13749..14288
                     /locus_tag="ACHVC7_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR2115111.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHVC7_001004"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHVC7_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR2115112.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHVC7_001005"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHVC7_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR2115113.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHVC7_001006"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHVC7_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR2115114.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHVC7_001007"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHVC7_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR2115115.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHVC7_001008"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHVC7_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR2115116.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHVC7_001009"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHVC7_001009"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR2115117.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHVC7_001010"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHVC7_001010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR2115118.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHVC7_001011"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHVC7_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR2115119.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHVC7_001012"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHVC7_001012"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR2115120.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHVC7_001013"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHVC7_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR2115121.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHVC7_001014"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHVC7_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR2115122.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHVC7_001015"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHVC7_001015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR2115123.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHVC7_001016"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHVC7_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR2115124.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHVC7_001017"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHVC7_001017"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR2115125.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..>30986
                     /locus_tag="ACHVC7_001018"
     CDS             30847..>30986
                     /locus_tag="ACHVC7_001018"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2115126.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLL"
BASE COUNT         7623 a         8408 c         7828 g         7127 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgct
//
LOCUS       DAZPLM010000026        30133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPLM010000026 DAZPLM010000000
VERSION     DAZPLM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.026"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="ACHVC7_001019"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="ACHVC7_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR2115127.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="ACHVC7_001020"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="ACHVC7_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR2115128.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="ACHVC7_001021"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="ACHVC7_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR2115129.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="ACHVC7_001022"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="ACHVC7_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR2115130.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="ACHVC7_001023"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="ACHVC7_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR2115131.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHVC7_001024"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHVC7_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR2115132.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="ACHVC7_001025"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="ACHVC7_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR2115133.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHVC7_001026"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHVC7_001026"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR2115134.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHVC7_001027"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHVC7_001027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR2115135.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHVC7_001028"
     CDS             complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHVC7_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR2115136.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHVC7_001029"
     CDS             complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHVC7_001029"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR2115137.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHVC7_001030"
     CDS             complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHVC7_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR2115138.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHVC7_001031"
     CDS             complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHVC7_001031"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR2115139.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHVC7_001032"
     CDS             complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHVC7_001032"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR2115140.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHVC7_001033"
     CDS             complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHVC7_001033"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR2115141.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18342..18442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18495..18971)
                     /locus_tag="ACHVC7_001034"
     CDS             complement(18495..18971)
                     /locus_tag="ACHVC7_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR2115142.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19066..19839
                     /gene="nudC"
                     /locus_tag="ACHVC7_001035"
     CDS             19066..19839
                     /gene="nudC"
                     /locus_tag="ACHVC7_001035"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR2115143.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPLSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19879..20943
                     /gene="hemE"
                     /locus_tag="ACHVC7_001036"
     CDS             19879..20943
                     /gene="hemE"
                     /locus_tag="ACHVC7_001036"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR2115144.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20953..21624
                     /gene="nfi"
                     /locus_tag="ACHVC7_001037"
     CDS             20953..21624
                     /gene="nfi"
                     /locus_tag="ACHVC7_001037"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR2115145.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21667..22257
                     /locus_tag="ACHVC7_001038"
     CDS             21667..22257
                     /locus_tag="ACHVC7_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR2115146.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22444..22716
                     /gene="hupA"
                     /locus_tag="ACHVC7_001039"
     CDS             22444..22716
                     /gene="hupA"
                     /locus_tag="ACHVC7_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR2115147.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22729..23424
                     /locus_tag="ACHVC7_001040"
     CDS             22729..23424
                     /locus_tag="ACHVC7_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR2115148.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="ACHVC7_001041"
     CDS             complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="ACHVC7_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR2115149.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24089..25465
                     /gene="zraS"
                     /locus_tag="ACHVC7_001042"
     CDS             24089..25465
                     /gene="zraS"
                     /locus_tag="ACHVC7_001042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR2115150.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25462..26787
                     /gene="zraR"
                     /locus_tag="ACHVC7_001043"
     CDS             25462..26787
                     /gene="zraR"
                     /locus_tag="ACHVC7_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR2115151.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26784..28073)
                     /gene="purD"
                     /locus_tag="ACHVC7_001044"
     CDS             complement(26784..28073)
                     /gene="purD"
                     /locus_tag="ACHVC7_001044"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR2115152.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28085..29674)
                     /gene="purH"
                     /locus_tag="ACHVC7_001045"
     CDS             complement(28085..29674)
                     /gene="purH"
                     /locus_tag="ACHVC7_001045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR2115153.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7051 a         7860 c         8305 g         6917 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tacgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt ccttgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccaca tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgctt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggtaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaatc cctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tggtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggagctgg tgccggaacg cctgcgtggt gaaactgcat ctttcgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgt tggctaagct gagccagtct ggtcacaagc gtattgaaac gctgttcacc
     5341 aacgacctgg atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacctgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc tgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggaca tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc gggtggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcatta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactcttt gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaggtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctttctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgtgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtttgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tgtatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccactggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg ttctgggtct
     9961 gtactatatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgcaaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtga ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac
    11581 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc
    11881 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg
    12721 gcaaattctt cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc
    12781 cctggacaac attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct
    12841 tgcgtttttc atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat
    12901 agtctttgcg aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc
    12961 tgagcggtta acgcggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt
    13021 tccaactcgg ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg
    13081 ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt
    13141 gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt
    13201 tcatccataa tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag
    13261 acagagtaac ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata
    13321 tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca
    13381 atccccgcac gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg
    13441 cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc
    13501 ccatccagac caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc
    13561 atttgtagtg aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc
    13621 cccactttcg cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt
    13681 atagcggcgc tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta
    13741 ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg
    13801 acgtagaaac taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ccgttgggcc
    13861 agtggttcca gatagccact ggcggctggc gataacagcg ccatcatatc gtcgcgggtg
    13921 agttgcgagg catttagcgc ccgttctacg tcagcagccg ttttgccgtt gatacgcagg
    13981 cggatgtcgt cccagtccag ttgtcgccag cgatcgctga aggttttcat gccgatgcct
    14041 ccagaaatcc agtcagcggg ctggtggcat gagcaaaatg actgcggctg cccggtccgg
    14101 actgacgtgc cagcaggcct gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg
    14161 gatcgtccgc tacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccaggg
    14221 cctgcgcggc atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct
    14281 gctggataat aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg
    14341 cgccgagcgg catcaccgct gcacagccga cttcttccag acgtttacac aataccggat
    14401 cggccccgca gtaaggcagc acgacaaatc cctgttgtac cagcatttcg gcggctttca
    14461 gagtttcgat gggatcgggc aacagccagc gggcgtcagg gtgaatctct aattttaacc
    14521 agtttgtgcc taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt
    14581 tcgccccgga agtatttggc agcagggtca cacccgccgc gataagcggt tcgaggatag
    14641 cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg
    14701 aagcgcggat cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca
    14761 gatgtgaatc aaacgttttg tccgcaatac gtaacatctc aaccccctgc aataacctga
    14821 aaaagcagga tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg
    14881 atttgctgat taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt
    14941 tcgtgaacag tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt
    15001 gcttcctccg cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct
    15061 cgatttaccg tcgaacagtc ggagttctcc cgcaggtgtc tctataccgc ttaataactt
    15121 aatggcttcc agtgcctgca aagtgcccat aaccccgacc accgggccaa ccacgcccgc
    15181 cgtgcggcag ttgcgttctg gctcctggtt atctggccac aggcagcggt aacacccctg
    15241 ctcccagggc ggcgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat
    15301 aagcggcgtg ttgagtacca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc
    15361 ggtacagtcg agcaccacat cggcccgtgc aaccgcatct tttaacgcct cacccgttaa
    15421 ccgttgttgt aatgctgtca gttgaatatc gggattcaac tgtgtcagtc gctgatggct
    15481 gacctgcgat ttcgggcgat cgatatcttc agtggtaaag aggatttgtc gttgcagatt
    15541 gcttaaatgc acatcgtcgt catctgccag taccagcgtc ccgacgccag cccccgccag
    15601 gtatagcgca gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag
    15661 cagtttttgc tgcccgtcca gagcgatatc gtcgagcaga atttggcggc tataacgcat
    15721 aaagtcacgg tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac
    15781 gccagtctgc ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta
    15841 tcaccgcagg cgcgcgcgcc agactgatac cgccaatcgc cacggtggga taatccgcca
    15901 gtcgctcaac atgccgtgcc agctgttcca gcccctgcgg tgcagaaggc atctgtttgg
    15961 tttgcgtcgg gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt
    16021 cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat
    16081 tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga
    16141 tcgccagccg ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa
    16201 ttgccgccac gacatcggct tccacctctt cattgcgccg atctttgatg cgtagctgga
    16261 gagtacgtac gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt
    16321 acagtcctaa acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt
    16381 acgcaggtag atttctccgc ctctggcgcg gaagttctcc gacatatccg ccattcccac
    16441 ttcaatggtt tgcgcggcgg cgtaatcacg tacttcctgg ctgattttca tcgagcagaa
    16501 tttcggccca cacatggagc aaaaatgggc gactttacct gactcttgcg gcagggtttc
    16561 atcgtgataa gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg
    16621 aaattcgaag cgggctttcg acatggcgtt atcgcgaatt tgcgcgcctg gatgcccttt
    16681 cgccaggtca gcggcgtggg cggcaatctt ataggtgata agcccctgct taacatcttc
    16741 tttattaggc agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa
    16801 ccagccaatc atcgccgcac caatccccga cgtgaagtgg tcatagcccg gcgcaatatc
    16861 ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt
    16921 catattgcgg cggatcatct gcatcggcac gtggcctggg ccttcaatca tcacctgcac
    16981 gtcatattcc caggcaattt tggtcagttc gcccagcgta tgcagctcgg caaactgcgc
    17041 ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac
    17101 gtcataagcg gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg
    17161 atgatgggag aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag
    17221 gcgtttcgcg gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc
    17281 cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc
    17341 ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg
    17401 gctgttacgc aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat
    17461 caccgtatcc gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga
    17521 agaggtgacc gccgagttgc cgatattggc gttaactttt accaggaaat tgcgaccaat
    17581 aatcatcggc tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc
    17641 aacttcatca cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat
    17701 tcccggatgc tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg
    17761 gatggcgatg aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt
    17821 gacacggcgt cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc
    17881 gagaccatca tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc
    17941 ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt
    18001 aatggcgatc tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt
    18061 ttcttcgtac tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc
    18121 acgcatcggc acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg
    18181 aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg
    18241 ggtcagtttt gttgcagaca tagctcattc caaaaagtca aggacgtggc ttgtcagacg
    18301 acggatgaag caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc
    18361 ccttcgcagg tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt
    18421 taaggcactc cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt
    18481 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    18541 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    18601 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc
    18661 ataatctgtt gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    18721 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    18781 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    18841 tctttgccaa gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    18901 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    18961 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19021 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19081 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19141 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19201 atcggcgaat ggcaggggga acctgtttgg ttagtacaac agcagcggcg tcacgatatg
    19261 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgaggc
    19321 gtacaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    19381 tatccgagca aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg
    19441 caaatcgccc cctgcattat tgttgccatc cgtcgcgatg attcgatcct cctcgcccag
    19501 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    19561 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    19621 ttgcgttacg tgacttctca gccgtggccg tttcctctgt ctttaatgac cgcgtttatg
    19681 gcggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    19741 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    19801 gaagatactg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    19861 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    19921 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    19981 gaatataaag ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag
    20041 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc
    20101 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20161 gaaggtccgc gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt
    20221 ccggacccgg aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa
    20281 ctgaaaggcg aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcgacctac
    20341 atggtggaag gcggcagcag caaagcgttc accgtgatca aaaaaatgat gtatgccgat
    20401 ccgcaggcgc tgcacgctct actcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    20461 gcgcagatta aagccggtgc tcaggcagtg atgattttcg acacctgggg cggtgtgctt
    20521 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    20581 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    20641 tggctggaag cgatggcaga aaccggttgc gatgcgttgg gcctcgactg gacaacggat
    20701 atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg
    20761 tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt
    20821 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    20881 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca atatcaccgc
    20941 taaggagtga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct
    21001 gtgatccgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg agccgatgtc
    21061 gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa atatccctcg
    21121 cttgagctgg tggagtataa agttgcccgc atcgccacca ccatgcctta cattccaggt
    21181 tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag
    21241 ccggatttag tgtttgtcga tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc
    21301 agccattttg gtttgatggt ggatgtgcca acgatcggcg tggcgaaaaa acggctctgc
    21361 ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc
    21421 gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc
    21481 ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat
    21541 cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg
    21601 cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg tatttgagaa
    21661 ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag ctggcagcat
    21721 gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat gttctgccag
    21781 caaaccggtt ttggtgatgg gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg
    21841 ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt tgaagaagcg
    21901 attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg
    21961 gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa
    22021 gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa
    22081 atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga cattcagtgg
    22141 gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt
    22201 agggcagacc tgcgtgaggc gggtgagagc aatattggta taatttttca gcaataagac
    22261 cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt cgccccccgt
    22321 ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc
    22381 tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt gtaaggataa
    22441 cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact gtccaaaacc
    22501 caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct gaaagaaggc
    22561 gatgctgtac aactggttgg tttcggtacc ttcaaagtga accaccgcgc tgagcgtact
    22621 ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt accggcattt
    22681 gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt gaacagtttt
    22741 aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat gctggctggc
    22801 gctctgcttc tcactgcctg tagtcataac tcttcacttc ctccctttac cgccagtgga
    22861 tttgctgaag accagggcgc ggtacgcatc tggcgaaaag acagcggcga taatgtgcat
    22921 ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg
    22981 cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc ggtgaatatt
    23041 cgcgcgcgtt ttgacgatcg cggtgatctg agctttatgc aacgtgaatc cgatggggaa
    23101 aagcagcagc tttctaacga ccaaatcgat ttataccgtt atcgtgctga tcagatccgc
    23161 cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg
    23221 atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt agattcgcag
    23281 gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg
    23341 ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt ctgtcgctgg
    23401 caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga ccgccaccac
    23461 cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct tcagccatcg
    23521 cgatatctcg tttcacccgt aactcatcta acgactgacg caaattctcc atctctttgg
    23581 cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca ttgtattcat
    23641 aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag tcattatgga
    23701 ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac
    23761 cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa agcgccatca
    23821 ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt gtcttacttc
    23881 gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat ctggcatgat
    23941 aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc attgaaactt
    24001 aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat ttctgctgtc
    24061 agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta aagactcctt
    24121 agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag ggttgtttgc
    24181 ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa cgctgctgga
    24241 aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca tggggatgcg
    24301 catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc ctggagtacg
    24361 ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact ccggcatggt
    24421 gggaaaacag ctttattccc cgcaggaaat gcagcagtta catccgggag atgaagaagc
    24481 gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc gccagtttca
    24541 accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg ccgcgacacc
    24601 acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag atcgtgagca
    24661 gagaaacacc ctgattatcc tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc
    24721 attcttctgg tatcgccgct atttgcgctc gcgccagctt ctacaagatg aaatgaagcg
    24781 caaagagaag ctggtggcgc tggggcatct tgcggcaggc gttgcccacg aaatccgtaa
    24841 cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg
    24901 agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt
    24961 aagcgagctg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg tggatctcaa
    25021 cacgctgatt aaccactcat tacagctggt aagccaggat gcgaacagcc gggagatcca
    25081 gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg acaggctgac
    25141 tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc atggcgtgat
    25201 tagcgtgacg gccagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa
    25261 gggaattgcg gcagatcagc ttgatgccat cttcactccg tacttcacca ctaaagccga
    25321 aggcaccgga ctggggctgg cggtcgtgca taatattgtt gaacaacacg gtggtacaat
    25381 tcaggtcgca agccaggagg gaaaaggcgc aacgttcacc ctctggcttc cggtcactat
    25441 tacgcgtaag ggcccacaag gatgacgcac gataatatcg atattctggt ggtggatgat
    25501 gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta taacgtcgcg
    25561 ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg aacaggtttt tgatcttgtg
    25621 ctttgcgatg tgcgaatggc ggagatggac ggcattgcca cgctaaaaga gatcaaagcg
    25681 ttaaatccgg cgattccggt gctgattatg accgcatact ccagcgtcga gacggcggta
    25741 gaagcgttga aaaccggggc gctggattat ctcatcaagc cgctggattt cgataacctg
    25801 caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgacgctga aacgcctgcg
    25861 gtgtctgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt
    25921 gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga ttccggcacc
    25981 ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga aaaaccgctg
    26041 gtaacgctca actgtgcggc actcaacgaa tccttgctgg aatctgaatt gttcggtcac
    26101 gaaaaagggg cgttcactgg agccgataga cgccgggaag ggcgctttgt tgaggcggac
    26161 ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg
    26221 ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac tatctcggtt
    26281 gatgtccggt tgattgcggc gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt
    26341 tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg
    26401 caacggcggg aagatatccc tctgctggct gtccattttc ttcagcgctt tgccgagcgt
    26461 aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat ccactacgac
    26521 tggccgggaa atattcgtga actggaaaac gcggtggaac gggcggtggt gctgctgacc
    26581 ggggaatata tttccgaacg cgagctgccg ctggcgattg ccggtacgcc gatcccactg
    26641 gggcaaagtc aggatattca gccgctggtg gaagtcgaaa aagaagtgat tctggcggca
    26701 ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa
    26761 acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata
    26821 tctttccggc agaagcagtc gtcccagtga atatcggtca ttaaggcata agcgcgtttc
    26881 tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc
    26941 actacctgct cgttatccgc cagtttcgtg cccgcgtgga acactttgcc gcctgccact
    27001 tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt aatcgcccgg atatccaccc
    27061 gcagccatca ccacgccgag agaagcacgc tcatcccact ctgacgtttt ctcgtccagt
    27121 ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg cagcataatc
    27181 ggctgggttt ctggatcgcc aaagcggcag ttaaattcga taaccttcgg attgccctgt
    27241 ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg
    27301 cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc atcggttact
    27361 accggcgcgg gggagtaagc gcccatcccg cccgtgtttg gtccggtatc tttatcgcct
    27421 acgcgtttgt gatcctggct ggtggccatc ggcagcacat gctcgccgtc taccatcacg
    27481 ataaagctcg cttcttcgcc gtcgaggaac tcttcgataa cgatgcgatg acccgcgtcg
    27541 ccaaaagcgt tgcccgccag catatcgtga acagccgctt cggcttcttc cagcgtcatc
    27601 gccacgataa cgcctttccc ggctgccaga ccgtccgctt taatgacgat cggcgcacct
    27661 ttctcacgca gatacgccag cgcaggttct acctcggtga agttctggta ttccgctgta
    27721 gggatcttat ggcgggccag gaaatctttg gtaaacgctt tcgaaccttc cagttgcgcg
    27781 gcacctgcgg ttgggccgaa gattttcatc ccggcggcgc ggaaggtatc gaccacgcct
    27841 ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa
    27901 tccaacagcg ccgggatatc ggtcacgccg atagcaacgt tttgcagcgc gggttccagc
    27961 gcagtgcctg cattacccgg agcaacaaaa acagtttcaa ccagcggcga ctgcgccgct
    28021 ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt catctattgc
    28081 tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg
    28141 cggcggcaat cacttcgtca tcacggatag aaccgccagg ctggattacg caggtcacgc
    28201 ctgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg
    28261 aggaaccttt cacttccagg ccttcatcgg cagctttaat accggcgatt ttcgcggagt
    28321 acacgcggct catctggccc gcgccaatcc cgatggtcat attgtttttg gcatagacga
    28381 tagcgttgga tttcacgaac ttcgccactt tccagcagaa cagcgcatca cgcagttcct
    28441 gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc atgcccaggt
    28501 cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct
    28561 cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg attttcaggg
    28621 cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa atgatggcct
    28681 gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat gccgccgaat gcggaggttg
    28741 ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata gccacgccgc
    28801 aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc acgcactcca
    28861 gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga acctgggttg
    28921 cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc tgctggtggc
    28981 tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc gtgcgtggga
    29041 agcgaccggc ggcttctttg ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt
    29101 tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac
    29161 gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg gcgtcgtagt
    29221 cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag cgcaccatgg
    29281 tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcagcct tcacgagcaa
    29341 cagtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg atctgatgtt
    29401 cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt actttcggat
    29461 gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg
    29521 ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac agcagctcca
    29581 caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca gaaacactga
    29641 gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctggat ttgactatta
    29701 cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa cggggcatcc
    29761 ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt
    29821 caatggtgat cacaattttg acagtggtta ccgtgggaaa aatacagaaa ttacattgat
    29881 gattgtggat aactctgtgc gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc
    29941 agtcagtcat ttttctgcaa tttttctatt gcggcctgcg gagaactccc tataatgcgc
    30001 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
    30061 aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    30121 cagtgcgcta aag
//
LOCUS       DAZPLM010000027        30112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPLM010000027 DAZPLM010000000
VERSION     DAZPLM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.027"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1033
                     /locus_tag="ACHVC7_001046"
     CDS             <1..1033
                     /locus_tag="ACHVC7_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR2115154.1"
                     /translation="DSYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYAL
                     LVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYNLLNTRDMILNELHQHVFL
                     KDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDGNKDSRINKNVIVLAGNHE
                     INFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVLTSHHGIIRNEENECYCLG
                     ALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNNTTA
                     FRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGV
                     PGLSCFQPQ"
     gene            1295..2917
                     /locus_tag="ACHVC7_001047"
     CDS             1295..2917
                     /locus_tag="ACHVC7_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR2115155.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3107..3220
                     /gene="yhiY"
                     /locus_tag="ACHVC7_001048"
     CDS             3107..3220
                     /gene="yhiY"
                     /locus_tag="ACHVC7_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR2115156.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3283..4350
                     /locus_tag="ACHVC7_001049"
     CDS             3283..4350
                     /locus_tag="ACHVC7_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR2115157.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4347..7082
                     /gene="rbbA"
                     /locus_tag="ACHVC7_001050"
     CDS             4347..7082
                     /gene="rbbA"
                     /locus_tag="ACHVC7_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR2115158.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7082..8206
                     /locus_tag="ACHVC7_001051"
     CDS             7082..8206
                     /locus_tag="ACHVC7_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2115159.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8279..8551
                     /locus_tag="ACHVC7_001052"
     CDS             8279..8551
                     /locus_tag="ACHVC7_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR2115160.1"
                     /translation="MRTKVQALRKKKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIK
                     EGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8548..8907
                     /locus_tag="ACHVC7_001053"
     CDS             8548..8907
                     /locus_tag="ACHVC7_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR2115161.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9027..9428)
                     /gene="nikR"
                     /locus_tag="ACHVC7_001054"
     CDS             complement(9027..9428)
                     /gene="nikR"
                     /locus_tag="ACHVC7_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR2115162.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9434..10240)
                     /gene="nikE"
                     /locus_tag="ACHVC7_001055"
     CDS             complement(9434..10240)
                     /gene="nikE"
                     /locus_tag="ACHVC7_001055"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR2115163.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10237..11001)
                     /gene="nikD"
                     /locus_tag="ACHVC7_001056"
     CDS             complement(10237..11001)
                     /gene="nikD"
                     /locus_tag="ACHVC7_001056"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR2115164.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(11001..11834)
                     /gene="nikC"
                     /locus_tag="ACHVC7_001057"
     CDS             complement(11001..11834)
                     /gene="nikC"
                     /locus_tag="ACHVC7_001057"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR2115165.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11831..12775)
                     /gene="nikB"
                     /locus_tag="ACHVC7_001058"
     CDS             complement(11831..12775)
                     /gene="nikB"
                     /locus_tag="ACHVC7_001058"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR2115166.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12775..14349)
                     /gene="nikA"
                     /locus_tag="ACHVC7_001059"
     CDS             complement(12775..14349)
                     /gene="nikA"
                     /locus_tag="ACHVC7_001059"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2115167.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14460..15047)
                     /gene="acpT"
                     /locus_tag="ACHVC7_001060"
     CDS             complement(14460..15047)
                     /gene="acpT"
                     /locus_tag="ACHVC7_001060"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR2115168.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15102..16151)
                     /locus_tag="ACHVC7_001061"
     CDS             complement(15102..16151)
                     /locus_tag="ACHVC7_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR2115169.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16283..17500
                     /locus_tag="ACHVC7_001062"
     CDS             16283..17500
                     /locus_tag="ACHVC7_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115170.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17504..18061)
                     /gene="dcrB"
                     /locus_tag="ACHVC7_001063"
     CDS             complement(17504..18061)
                     /gene="dcrB"
                     /locus_tag="ACHVC7_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR2115171.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18134..18799)
                     /locus_tag="ACHVC7_001064"
     CDS             complement(18134..18799)
                     /locus_tag="ACHVC7_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR2115172.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            19020..19265
                     /gene="tusA"
                     /locus_tag="ACHVC7_001065"
     CDS             19020..19265
                     /gene="tusA"
                     /locus_tag="ACHVC7_001065"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR2115173.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19367..21565)
                     /gene="zntA"
                     /locus_tag="ACHVC7_001066"
     CDS             complement(19367..21565)
                     /gene="zntA"
                     /locus_tag="ACHVC7_001066"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR2115174.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21639..22265)
                     /locus_tag="ACHVC7_001067"
     CDS             complement(21639..22265)
                     /locus_tag="ACHVC7_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR2115175.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            22406..22765
                     /locus_tag="ACHVC7_001068"
     CDS             22406..22765
                     /locus_tag="ACHVC7_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR2115176.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22768..23037)
                     /locus_tag="ACHVC7_001069"
     CDS             complement(22768..23037)
                     /locus_tag="ACHVC7_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR2115177.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23027..23623)
                     /gene="rsmD"
                     /locus_tag="ACHVC7_001070"
     CDS             complement(23027..23623)
                     /gene="rsmD"
                     /locus_tag="ACHVC7_001070"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR2115178.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23773..25269
                     /gene="ftsY"
                     /locus_tag="ACHVC7_001071"
     CDS             23773..25269
                     /gene="ftsY"
                     /locus_tag="ACHVC7_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR2115179.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25272..25940
                     /gene="ftsE"
                     /locus_tag="ACHVC7_001072"
     CDS             25272..25940
                     /gene="ftsE"
                     /locus_tag="ACHVC7_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR2115180.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            25933..26991
                     /gene="ftsX"
                     /locus_tag="ACHVC7_001073"
     CDS             25933..26991
                     /gene="ftsX"
                     /locus_tag="ACHVC7_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR2115181.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27236..28090
                     /gene="rpoH"
                     /locus_tag="ACHVC7_001074"
     CDS             27236..28090
                     /gene="rpoH"
                     /locus_tag="ACHVC7_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR2115182.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28361..29464
                     /gene="livJ"
                     /locus_tag="ACHVC7_001075"
     CDS             28361..29464
                     /gene="livJ"
                     /locus_tag="ACHVC7_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR2115183.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29663..30046)
                     /gene="panM"
                     /locus_tag="ACHVC7_001076"
     CDS             complement(29663..30046)
                     /gene="panM"
                     /locus_tag="ACHVC7_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR2115184.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7370 a         7619 c         8164 g         6959 t
ORIGIN      
        1 tgatagctat gttaaatctg tgttttctaa aatagacttt tataaacaat gtggtgtgat
       61 taacccacaa aatgccaaca cggcttattt tggcgataca gatggaagag taggcgctgt
      121 actttatgca ttgctcgttt cagggcatat aggcatcagg gaaaagggtt ggagtttatt
      181 atgtgaatta ttaaaacacg aaaacatggc ttcatctgca tatatacata aaaataataa
      241 attgctctac aatttattaa atacgagaga catgatattg aatgagttgc atcaacatgt
      301 ttttttaaag gacgatgcga taaccccttg tatcttcctt ggggatcata caggcgatag
      361 atttagtact atttttggtg ataaatatat ccttactttg ctaaattcta tgcgaaatat
      421 ggatggtaat aaagatagcc gtattaataa aaatgtcatc gttctggctg gaaatcatga
      481 aatcaatttt aatggtaact atactgcacg actagaaaat tataaactct ctgccggtga
      541 tacgtataac ttaattaaaa cgctcgatgt ttgtcattat gactcagaaa cgaaagtttt
      601 aaccagtcat cacggaataa taagaaatga agaaaatgaa tgttattgtc tcggtgcttt
      661 gcaggttcca tttaatcaga tgaaagaccc aattgatcct gaggaattag caaatatatt
      721 taataaaaaa cataaacaac acatggatga tcgtcttatc catctaatta gaagcaatgc
      781 tgtagcatcc acacctgtat atgaaaatta ttttaataac actactgctt tcagaccaaa
      841 acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga aatatggcca
      901 ttatggatta ggggtagatc agcatcaaaa aattgataat tataccatgg ggctaaattc
      961 ctggaagata gcacctaacg aacgtggtga taaaaaaggt gtcccagggc ttagttgttt
     1021 tcaacctcaa taaaacttca tgagtgctgt ggtatctctt tttaattttg ccagtatttc
     1081 ataggttttc ttaatcgatt gtagaggcat gttctgatga cgcctctaca atctttaggg
     1141 actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa tgatttgtgc
     1201 aaaatataat tattttccca tcgaacatga ggtgtatgtc ataagattaa atgttaacta
     1261 tgtattcctc gcggtaaata atatgaggta aaagatgaaa attggaactg tggcaggcac
     1321 taacgacagt acaacgacaa tcacaacaaa tgatatggtg caagagcatg ttaccaactt
     1381 cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat caagtattgc
     1441 caggacaatg ctcggtgagg ttgtggaaaa aatagatgat tggcaaatag aacggttcca
     1501 acaatctatt caagatgaca aaatttcatt taccatacaa acgaatcatt cagaaaaata
     1561 tagtatgcta tcgggcatgc gcgctcatat tctacgtagg aataactgtt atcagtttat
     1621 tgtgacaatt aatagtaaaa attacggttg ccctcttgat aataccgata ttaattggtg
     1681 cagcattgtt tatttactta ataatatgac aataaatgat aatgctaatg atgttgcagt
     1741 tactgagtcg tacaaaccca tctggaactg gaaaatatcg caatacaatg tatttgatat
     1801 taaattcgaa acaatgataa aaccccagtt cgctgacaga acctatttct ccaattgttc
     1861 acctgttgat cccacaagta ccagaccgac ctattttggc gataccgatg gctcagtcgg
     1921 tgcagttttg tacgcgcttt ttgcaacggg acatttggga atcatggctg aaggagaaaa
     1981 tttccttagc cagttgctta atatagaaga tgaagttctc aacgtattgc taagggagaa
     2041 ctttaatgag caacttgaca ctaatgttaa tataataatc agtatcctga acagaagaga
     2101 cattgtttta gaaagtcttc agccttattt ggttattaat aaagacgcag tcacgccatg
     2161 tacgtttctg ggcgaccaga ctggcgatcg atttagtaat atctgtggcg ataaatttat
     2221 catcgattta ttaaaacgca taatgagtat caatgaaaac gttcatgtat tagctggaaa
     2281 tcatgaaacg aattgcaatg gtaactatat gcagaacttc acgcgcatga aaccacttga
     2341 tgaggatacc tactctggga taaaagatta tcctgtatgt ttctatgacc ccaaatacaa
     2401 aataatggca aatcatcatg ggataacttt tgatgatcag cgaaaacgtt acattatagg
     2461 accgataaca gtctctagtg atgaaatgac caatgctctc gatccggtcg aattagcggc
     2521 aattatcaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa atagccgggc
     2581 tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca gacctaaact
     2641 tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg cacataacgg
     2701 taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta acgcccgtga
     2761 cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc ctggtgcagg
     2821 gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa atggacttat
     2881 gcctttgatc agaaggcgca caatgttaca gctctgaacc atagacaaca tgaatattaa
     2941 tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg ttcctgcaac
     3001 tgaacaggcg tgaaacgcta tccaacagga tggataccgt tttgtcagcc agttttatag
     3061 atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
     3121 actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg ctggtcgtat
     3181 ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg gcggcagttg
     3241 taacacgcgt cgtttgcttg cagggattat ggcgggtcga ttatggataa gagtaagcgc
     3301 catctggcgt ggtgggttgt cgggttactg gtggtggctg ccgtcgtggc gtggtggctg
     3361 ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat tgaagcgacg
     3421 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
     3481 cagtttgttc gcgaaggcga agtgctggcg aagatggata ctcgcgtgtt gcaggagcag
     3541 cgactggaag ccatcgcgca aatcaaagaa gcacaaagtg ccgttgctgc cgcgcaggct
     3601 ttgctggagc aacggcaaag cgaaactcgt gccgcgcagt cgctggttaa tcaacgccag
     3661 tccgaactgg actccgtggc taaacgtcat acgcgttccc gctcgctcgc ccaacgaggg
     3721 gctatttctg cgcaacagct ggatgacgat cgcgccgccg ctgagagcgc ccgagctgcg
     3781 ctggaatcgg caaaagctca ggtatcggct tctaaagcgg ctatagaagc ggcccgtacc
     3841 aatatcattc aggcccaaac tcgcgtcgaa gccgcgcaag ccactgaacg gcgtattgcc
     3901 gcagatatcg atgacagtga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
     3961 gctgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt cgatctcagc
     4021 gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctgaa acttggcggt
     4081 gaagcccggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac catcagtttt
     4141 gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga acggctgaaa
     4201 ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
     4261 gtcaaaaccg gtttaccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
     4321 gacgacctcg tggtgaggtt gccgcaatga cgcatctgga actggttccc gtcccgcctg
     4381 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaaa cgttgcgctg aacaatatta
     4441 ctctcgatat tccggcccgc tgtatggtcg ggctgattgg tccggatggc gtcgggaagt
     4501 cgagcttatt gtcgttgatt tccggtgccc gcgtcattga gcagggcaat gtgatggtgc
     4561 tgggcggcga tatgcgcgac ccgaggcatc gccgcgacgt ctgcccgcgc atcgcctgga
     4621 tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa aacgtcgatt
     4681 ttttcgctcg cctgttcggt cacgacaaag cggagcggga agtgcgaatt aatgagctgc
     4741 tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc tccggcggga
     4801 tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg ttgatccttg
     4861 atgagccaac aacgggggtt gacccgctct cccgctccca gttctgggat ctgatcgaca
     4921 gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat atggaagagg
     4981 ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca actggcagcg
     5041 ccgaagagct acggcagcaa acgcaaagcg ctacgctgga agaagcattt ataaatctgt
     5101 taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat caacctgaaa
     5161 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
     5221 ttgatcacgt taatttccgc attccacgcg gggagatttt tggttttctt ggttcgaacg
     5281 gctgcggtaa atccaccacc atgaaaatgc tcaccggact gctgcccgcc agcgaaggtg
     5341 aggcgtggct gttcgggcaa ccggttgatc caaaagatat cgatacccgc cgtcgggtgg
     5401 gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa aaccttgagt
     5461 tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcaagagtg gctgaaatga
     5521 gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg ccgctcggca
     5581 ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg ttaatcctcg
     5641 atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag ttgatggtcg
     5701 atctctcgcg ccaggacaaa gtgactatct tcatctctac ccactttatg aacgaagcgg
     5761 aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc ggtacaccgc
     5821 aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc gcctatttgc
     5881 aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac gacaccaccc
     5941 acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
     6001 tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg gtgatcctga
     6061 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgctcg
     6121 accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt tcccgttact
     6181 ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg cgtgcgggcg
     6241 atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg cgtggtacgc
     6301 ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa acggtaaaag
     6361 gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga caatcgacac
     6421 ccgccagcca aagcgggctg atgaacattg agacgcgcta tcgctataac ccggacgtaa
     6481 aaagcctgcc agcgattgtt ccggcggtga tcccgcttct gctgatgatg atcccgtcaa
     6541 tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc aacctttacg
     6601 tgacccccac cacccgtagc gaatttttgc ttggcaaaca gctgccgtac atcgcgctgg
     6661 ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc gtaccgcata
     6721 aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt gccaccggaa
     6781 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc ggaacggcga
     6841 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
     6901 aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
     6961 gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt atcccgttgc
     7021 tgatagccat cccgctggtg atgggcttaa gcatcctgct gctgaaaaaa caggagggat
     7081 gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca gtctgctcgg
     7141 tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
     7201 gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata tggatcaatc
     7261 gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc caccggagat
     7321 gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct tcgcgataaa
     7381 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata ttcaggtgaa
     7441 cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
     7501 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
     7561 gctggaaacc cggatgcgct ttaacccgaa cctcgatccc gcgtggtttg gcggggtgat
     7621 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
     7681 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
     7741 catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg gattatcgct
     7801 ggtgctgatg gcgaaaggtg tactgggcgt accgattgaa ggctcgatcc cgctgtttat
     7861 gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta tggggacgat
     7921 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
     7981 gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
     8041 gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc ggggtgccgg
     8101 attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg cgtttttcac
     8161 cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa gggaaacttg
     8221 acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag gacatgccat
     8281 gagaaccaag gtacaggctt tacggaagaa aaaaaataca ttggatcaaa tatttaaaac
     8341 tcctgttcct caagggatca agtggtctga tatagagtca ctggttaaag cattaggcgg
     8401 agaaattaag gaaggaagag gttcgcgttg taagttcata ctaaatatga gcgttgcgtg
     8461 tttccatcgg cctcatccgt cgccagatac cgataaaggc gctgtagaaa gcgtgcgtga
     8521 ctggttacta agtatagggg taaaaccatg atcaaactga agacgccaaa ctctatggag
     8581 attgccgggc aaccagctgt tattacctac gtgccagaac tgaatgcatt tcgtggcaaa
     8641 ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa
     8701 gaaggggaac tttccctgcg agagtatctt gaggattgta aagctgcggg tattgagccg
     8761 tatgcgcgta cagaaaagat taagaccttt acgttacgct atccagaatc gctgagtgaa
     8821 cgccttaata atgcggctgc gcagcaacaa gtctctgtta atacctatat tattgaaaca
     8881 ctgaatgagc gtttgaatca tttataaatt ttagcggcgt acattacgca gaatatctgc
     8941 ctttacagga tctatctatg caactgaact taattttata aattaagttc agttaatatc
     9001 ttttctttcc tcgtttcaga aataactcaa tcttccttcg gcaagcactg caaatgccca
     9061 tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc gcccatatca
     9121 cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg cagcgtggcg
     9181 acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa gtcgcgtttt
     9241 tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg ctgagtggcc
     9301 tcctgggcca gggcgctacg cagaatatcg cggatagctt cggaacggtt gttataacca
     9361 cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt gatggtgact
     9421 cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat gcgggtaata
     9481 ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct cccaccacct
     9541 gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag cgttccacca
     9601 ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt agctttttca
     9661 gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct tcatccagaa
     9721 tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg agctggccgc
     9781 cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc gccttcagca
     9841 tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg cgcatcggtt
     9901 cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag tcctgaaata
     9961 ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc gccagcggtt
    10021 cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc aaccgcgcga
    10081 gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg cttttcaggg
    10141 tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat ccaccgtggg
    10201 catagtgatg ggaaaggccg gagacgttaa gtaaagtcat gatgccagct ccataccgta
    10261 gagggcgaga tgagcggaaa ccaggctgcg cgttaccgca tgtttggggg cgttaaacag
    10321 cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca cgtcatccgc
    10381 cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg cttgtttttg
    10441 cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt
    10501 cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa tcatcatgcg
    10561 ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg cggcgttttc
    10621 cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt tccctaacgc
    10681 caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgtagc ggattaaagg cgctgcgcgg
    10741 gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg cagggataaa ccgctttgcc
    10801 atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc ccagcgtcgc
    10861 ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca cgcgcccgcg
    10921 ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg cgatattacg
    10981 tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg atccagatga
    11041 tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag cgccagcccc
    11101 ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt aatcatcacg
    11161 ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc cgcgacgtgc
    11221 agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat caccgcgcct
    11281 gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga cgccagcaca
    11341 aactcgcgtt ggcgtagtga aatcaccaag ctgcgcacca tgcgtgcata ccacgcccag
    11401 tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac gccaaccata
    11461 aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg catggtggcc
    11521 tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc taatgtcagc
    11581 accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgc catcagccgc
    11641 gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc cggcgaaagc
    11701 aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg gctggtgagc
    11761 gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca gcgggaagag
    11821 aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt
    11881 tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc agcgtaaagc
    11941 actgaatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc ccgacgcccg
    12001 gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg atgtgcatcc
    12061 ccacggcggt gatcatcggc agcgaggcat tgcgcagaat atggcggcgt tcggtctgtt
    12121 tgtcgttcag gccgcgcaga cgcgcccaag tgacgtgacg ctgaccggcg acgtccagca
    12181 tactggcgcg cagcagtcgc gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag
    12241 gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc agatacaccg
    12301 aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg ccaagaaacg
    12361 cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc cagataccga
    12421 gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc agcgtggcgg
    12481 gcaggaagtt cagcatatcg tccagcaccg ggcgttggct ggcgaatgag ataccaaagt
    12541 caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc tgatccagcc
    12601 cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta gataaacgca
    12661 gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg
    12721 cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc aacattaagg
    12781 tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt aggggatatt
    12841 acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca gataaaccgc
    12901 ctcgtcatgc agacgggtca aaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg
    12961 ggtcgccagc acttcgccga tctctttatc aatcagcggc ttgtcggcta atccttgctg
    13021 tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt gtggatcgta
    13081 tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata
    13141 gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc gcatatcagc
    13201 ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg acagttcaat
    13261 gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg tccaaccggc
    13321 tttttccagc aactctttcg ctttctgcgg atcgtactgg cgcggtttca ggccgagatt
    13381 agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc catataacgc
    13441 gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta ctgccagctc
    13501 gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg gctgtgacag
    13561 ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc
    13621 ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg
    13681 gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc agtagttttc
    13741 gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc acggtccggt
    13801 gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg agggagcgat
    13861 aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg cgcttttcag
    13921 ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt ttgccagctc
    13981 cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg ccgccgcctc
    14041 ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga aggtccaggt
    14101 tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg aaccgtctgc
    14161 ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt taggcgtgta
    14221 aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtagtgattt catctggtgc
    14281 agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc ggcggagtgt
    14341 ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat gacgatttta
    14401 agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg gtgggcggat
    14461 cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg ggtgggggtg
    14521 cagatcgcaa ggcttaaatt ttctagctga caatggctga ctgacagcga ggagtgatag
    14581 gtgctatcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac gatggcttct
    14641 ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatt
    14701 tcagcgtgtt ccccgaggct gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg
    14761 cgaatcactt cgatatcgca gccgacttcg ccttcatcgc tcaacagcag agcgatatcg
    14821 tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct
    14881 tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc
    14941 gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt
    15001 ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg
    15061 tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt
    15121 ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt ggtttccatc
    15181 cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg gccgagcagc
    15241 catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag gcgatggccc
    15301 atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa caatgcgccg
    15361 accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    15421 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    15481 agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc tgtccataaa
    15541 ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg gtgtaatcct
    15601 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    15661 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    15721 agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    15781 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    15841 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    15901 gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    15961 gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    16021 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    16081 aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt atcgggttga
    16141 gggatttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg agtcacgcca
    16201 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
    16261 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
    16321 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
    16381 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
    16441 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
    16501 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
    16561 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
    16621 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
    16681 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
    16741 gtggaacggc attgtcaatt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
    16801 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
    16861 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt
    16921 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
    16981 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt gggacggtgc
    17041 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
    17101 cggcattaac cgtatcggcg gcttaaacgt ggcgatgatt tgctttagcg ttgagataat
    17161 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaagtcg gcgtcttact
    17221 ggcgggggca gggttttcgc tggtgttccc ggcattgggc gtagtggcgg taaaagcggt
    17281 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
    17341 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
    17401 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
    17461 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
    17521 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct ggcagagtaa
    17581 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
    17641 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
    17701 ctttgttggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
    17761 tcgccaacac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
    17821 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
    17881 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
    17941 tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
    18001 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
    18061 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
    18121 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
    18181 tcaacagaat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
    18241 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
    18301 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
    18361 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
    18421 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
    18481 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
    18541 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
    18601 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
    18661 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
    18721 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
    18781 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
    18841 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
    18901 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
    18961 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
    19021 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
    19081 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcctggcgaa acgttgctga
    19141 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
    19201 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
    19261 gttgatgggg aaagattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
    19321 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
    19381 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
    19441 gccacaggcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc agccccagcg
    19501 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    19561 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
    19621 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
    19681 catcaccgac catcgccagg ggcgcatgtt gattcagctt ggtcaccgct ttgactttat
    19741 cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt gccgccgctg
    19801 cacgtggatt atcgccggtg aggatcactc ctttgacccc cagcgcgttc agttcactga
    19861 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
    19921 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
    19981 caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct
    20041 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga atggcgagtt
    20101 cagcaacctg tgcttcgcgt acgatggctt gcgccagtgg atgcgtcgcg ccttgctcga
    20161 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    20221 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
    20281 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgccgccg
    20341 ccagcccgga ggtaatcgcc gcaggcgttg agataactaa cgcacacggg cagccaatca
    20401 gcagcagcgt cagcccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
    20461 gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata cggctgaaac
    20521 ggtcgataaa ccgctcaatg ggggcgcgac gctcttcggc ttcttcgatc agtttcagaa
    20581 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    20641 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
    20701 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    20761 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
    20821 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
    20881 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
    20941 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
    21001 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgac
    21061 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
    21121 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
    21181 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
    21241 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
    21301 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
    21361 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
    21421 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
    21481 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
    21541 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
    21601 aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag tgagcggatg
    21661 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
    21721 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
    21781 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    21841 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
    21901 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
    21961 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    22021 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    22081 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    22141 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    22201 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    22261 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    22321 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt atgcttattt
    22381 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
    22441 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
    22501 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    22561 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
    22621 cgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    22681 gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
    22741 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    22801 cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg tcatctgcgg
    22861 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    22921 aaaaatcagc gccacgttaa cgaaaatatt tagcgggcgc gggaagggat gcaccagatt
    22981 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
    23041 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    23101 gatgtaacga ccagtttgcc ggaacggtgg gcagaccgtt ttcgacttcg ctttcgacat
    23161 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    23221 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg cctttttgcg
    23281 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    23341 gattcttaat taactgctga gaaactgcac gatccatctc aattaacgtg gcccccgcag
    23401 cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa tccagacatt
    23461 gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg
    23521 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    23581 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    23641 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    23701 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    23761 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    23821 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
    23881 gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga agagcagccg
    23941 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
    24001 caggttgttg aaagtgaaaa agcgcagcct gaagcggaag ttgttgcaca gccggaaccg
    24061 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
    24121 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
    24181 attgtcaaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
    24241 gcacaggcgc tggcagccga agcagcagaa gaggcggtga tggtggttcc tccggcagaa
    24301 gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccaaccaa agaaggtttc
    24361 ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc cggatttatc
    24421 agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga agagcagctt
    24481 ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct gacggaaggc
    24541 gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa agaagagatg
    24601 ggtgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg
    24661 atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa gctggcacgt
    24721 cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt ccgtgcagca
    24781 gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat tgcccagcat
    24841 accggggcgg attccgcctc tgttatcttc gacgccattc aggcagctaa agcgcgtaat
    24901 atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca cctgatggaa
    24961 gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc gcatgaagtt
    25021 atgctgacta ttgatgccag caccgggcag aacgcggtaa gccaggccaa actgttccac
    25081 gaagctgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc gaaaggcggg
    25141 gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg tgtcggcgaa
    25201 cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact ttttgcccga
    25261 gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt gggagacagg
    25321 cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg accggtcatt
    25381 ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg
    25441 ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc
    25501 tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac cgtactgtct
    25561 acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat attcgtcgcc
    25621 gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac ttccctattc
    25681 agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg aataagcccg
    25741 cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg gaagggattc
    25801 tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca acgcacgaca
    25861 tcaacctgat ttcacggcgt tcccatcgca tgctcaccct gagcgatggt cacttgcatg
    25921 gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt tggtgggcgt
    25981 ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa cgcaccgaaa
    26041 cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa cgaacaggtg
    26101 cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc cacgttttta
    26161 acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat ggtgtacaaa
    26221 aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt ttacttgcaa
    26281 aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc
    26341 gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg taactggtct
    26401 ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt ggcggtggtg
    26461 atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga tcgtatcacg
    26521 cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg tctggcggcg
    26581 ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt ggcggcagtg
    26641 ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga ctccatcaac
    26701 gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct gtatggtggc
    26761 gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat tctggtgctg
    26821 cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt tgatatcaat
    26881 ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat tggctgggtg
    26941 gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata ataaaagcgt
    27001 gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt tgtctcttcc
    27061 cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt gcattgaact
    27121 tgtggataaa atcactgtct gataaaacag tggatgataa cctcgttgct cttaagctct
    27181 ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga tttgaatgac
    27241 tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct acatccgggc
    27301 agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg aaaagctgca
    27361 ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc ggtttgttgt
    27421 tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga ttcaggaagg
    27481 taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg tgcgcctggt
    27541 ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc gtaactggcg
    27601 tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc tgcgtaaaac
    27661 caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc gtgaactggg
    27721 cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg acatgacctt
    27781 tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc tctatctgca
    27841 ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag agcaggcggc
    27901 aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca tcatccgcgc
    27961 gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc gttacggtgt
    28021 ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc gcgctgctat
    28081 agaagcgtaa tttccgctat taagcagaga accctggatg cgagtccggg gtttttgttt
    28141 tttgggcctc tacaataatc aatttcccct ccggcaaaac gccaatcccc acgcagattg
    28201 ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag
    28261 tatgctgcta aagcacgggt agccatgcat aaaacgaaat aaagtgctga acaacaacat
    28321 cacaacacac gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc
    28381 gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa
    28441 agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc aagagtttac
    28501 cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag gcaacaaact
    28561 gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa
    28621 agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat caacgcagcc
    28681 tgcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcgcc
    28741 ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca
    28801 ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt
    28861 tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg gcctgaagaa
    28921 aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctctac
    28981 gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttaccaccc
    29041 ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg
    29101 gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct
    29161 gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca ttgttgacgc
    29221 gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct acgccgcgct
    29281 gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct
    29341 gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga aaggcgatct
    29401 gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga cagatgcgaa
    29461 gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt
    29521 catgccggat gcggcgtaaa cgccttatcc ggcctataag atccaaagaa atcagtaaat
    29581 tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc gttggtcatc
    29641 acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc gccgtaaatc
    29701 ccagtgcctg cataaacgct gtcatcacac cgcgatcttc cacgcctgca tccgctatcc
    29761 accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat tgccccacac
    29821 cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg gtgccgctta
    29881 aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag atacggtggt
    29941 tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc aggtcaatcc
    30001 ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt aacccgtgta
    30061 aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc tc
//
LOCUS       DAZPLM010000028        29744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPLM010000028 DAZPLM010000000
VERSION     DAZPLM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.028"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            61..1689
                     /gene="glpA"
                     /locus_tag="ACHVC7_001077"
     CDS             61..1689
                     /gene="glpA"
                     /locus_tag="ACHVC7_001077"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR2115185.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            1679..2938
                     /gene="glpB"
                     /locus_tag="ACHVC7_001078"
     CDS             1679..2938
                     /gene="glpB"
                     /locus_tag="ACHVC7_001078"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR2115186.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            2935..4125
                     /gene="glpC"
                     /locus_tag="ACHVC7_001079"
     CDS             2935..4125
                     /gene="glpC"
                     /locus_tag="ACHVC7_001079"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR2115187.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            4318..5220
                     /locus_tag="ACHVC7_001080"
     CDS             4318..5220
                     /locus_tag="ACHVC7_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR2115188.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5261..6064)
                     /gene="yfaU"
                     /locus_tag="ACHVC7_001081"
     CDS             complement(5261..6064)
                     /gene="yfaU"
                     /locus_tag="ACHVC7_001081"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR2115189.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6082..7371)
                     /locus_tag="ACHVC7_001082"
     CDS             complement(6082..7371)
                     /locus_tag="ACHVC7_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115190.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(7428..8633)
                     /gene="rhmD"
                     /locus_tag="ACHVC7_001083"
     CDS             complement(7428..8633)
                     /gene="rhmD"
                     /locus_tag="ACHVC7_001083"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR2115191.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(8648..9430)
                     /locus_tag="ACHVC7_001084"
     CDS             complement(8648..9430)
                     /locus_tag="ACHVC7_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR2115192.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(9650..10852)
                     /locus_tag="ACHVC7_001085"
     CDS             complement(9650..10852)
                     /locus_tag="ACHVC7_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR2115193.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10952..11494)
                     /locus_tag="ACHVC7_001086"
     CDS             complement(10952..11494)
                     /locus_tag="ACHVC7_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR2115194.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            11773..12198
                     /gene="nudI"
                     /locus_tag="ACHVC7_001087"
     CDS             11773..12198
                     /gene="nudI"
                     /locus_tag="ACHVC7_001087"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR2115195.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(12237..12839)
                     /gene="ais"
                     /locus_tag="ACHVC7_001088"
     CDS             complement(12237..12839)
                     /gene="ais"
                     /locus_tag="ACHVC7_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR2115196.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            13147..14286
                     /gene="arnB"
                     /locus_tag="ACHVC7_001089"
     CDS             13147..14286
                     /gene="arnB"
                     /locus_tag="ACHVC7_001089"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR2115197.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            14290..15258
                     /gene="arnC"
                     /locus_tag="ACHVC7_001090"
     CDS             14290..15258
                     /gene="arnC"
                     /locus_tag="ACHVC7_001090"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR2115198.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            15258..17240
                     /gene="arnA"
                     /locus_tag="ACHVC7_001091"
     CDS             15258..17240
                     /gene="arnA"
                     /locus_tag="ACHVC7_001091"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR2115199.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            17237..18127
                     /gene="arnD"
                     /locus_tag="ACHVC7_001092"
     CDS             17237..18127
                     /gene="arnD"
                     /locus_tag="ACHVC7_001092"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR2115200.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            18127..19779
                     /gene="arnT"
                     /locus_tag="ACHVC7_001093"
     CDS             18127..19779
                     /gene="arnT"
                     /locus_tag="ACHVC7_001093"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR2115201.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            19776..20111
                     /gene="arnE"
                     /locus_tag="ACHVC7_001094"
     CDS             19776..20111
                     /gene="arnE"
                     /locus_tag="ACHVC7_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR2115202.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            20111..20497
                     /gene="arnF"
                     /locus_tag="ACHVC7_001095"
     CDS             20111..20497
                     /gene="arnF"
                     /locus_tag="ACHVC7_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR2115203.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(20491..20757)
                     /gene="pmrD"
                     /locus_tag="ACHVC7_001096"
     CDS             complement(20491..20757)
                     /gene="pmrD"
                     /locus_tag="ACHVC7_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR2115204.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(20867..22222)
                     /gene="menE"
                     /locus_tag="ACHVC7_001097"
     CDS             complement(20867..22222)
                     /gene="menE"
                     /locus_tag="ACHVC7_001097"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR2115205.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(22219..23181)
                     /gene="menC"
                     /locus_tag="ACHVC7_001098"
     CDS             complement(22219..23181)
                     /gene="menC"
                     /locus_tag="ACHVC7_001098"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR2115206.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(23181..24038)
                     /gene="menB"
                     /locus_tag="ACHVC7_001099"
     CDS             complement(23181..24038)
                     /gene="menB"
                     /locus_tag="ACHVC7_001099"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR2115207.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(24053..24811)
                     /gene="menH"
                     /locus_tag="ACHVC7_001100"
     CDS             complement(24053..24811)
                     /gene="menH"
                     /locus_tag="ACHVC7_001100"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR2115208.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(24808..26478)
                     /gene="menD"
                     /locus_tag="ACHVC7_001101"
     CDS             complement(24808..26478)
                     /gene="menD"
                     /locus_tag="ACHVC7_001101"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR2115209.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(26567..27862)
                     /gene="menF"
                     /locus_tag="ACHVC7_001102"
     CDS             complement(26567..27862)
                     /gene="menF"
                     /locus_tag="ACHVC7_001102"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR2115210.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27941..28246)
                     /gene="elaB"
                     /locus_tag="ACHVC7_001103"
     CDS             complement(27941..28246)
                     /gene="elaB"
                     /locus_tag="ACHVC7_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR2115211.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(28301..28762)
                     /locus_tag="ACHVC7_001104"
     CDS             complement(28301..28762)
                     /locus_tag="ACHVC7_001104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2115212.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            28827..29744
                     /gene="rbn"
                     /locus_tag="ACHVC7_001105"
     CDS             28827..29744
                     /gene="rbn"
                     /locus_tag="ACHVC7_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR2115213.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7136 a         8050 c         7541 g         7017 t
ORIGIN      
        1 caaacaattc atgtttttac tatggctaaa tggtaaaaaa cgaacttcag agggataaca
       61 atgaaaactc gcgactcgca atcaagtgac gtgattatca ttggcggcgg cgcaacggga
      121 gccgggattg cccgcgactg tgccctgcgc gggctgcgcg tgattttggt tgagcgccac
      181 gacatcgcaa ccggtgccac cgggcgtaac cacggcctgc tacacagcgg tgcgcgctat
      241 gcggtaaccg atgcggaatc ggcccgcgaa tgcattagtg aaaaccagat cctgaaacgc
      301 attgcacgtc actgcgttga accaaccaac ggcctgttta ttaccctgcc ggaagatgac
      361 ctctccttcc aggccacttt tattcgcgcc tgcgaagaag cagggatcag cgcagaagct
      421 atagacccgc agcaagcgcg cattatcgaa cctgccgtta acccggcatt gattggcgcg
      481 gtgaaagttc cggatggcac cgttgatcca tttcgtctga ccgcagcaaa catgctggat
      541 gccaaagaac acggtgccgt tatccttacc gctcatgaag tcacgggact gattcgtgaa
      601 ggcgcgacgg tgtgcggtgt tcgtgtacgt aaccatctca ccggcgaaac tcaggccctt
      661 catgcgcctg tcgtggttaa tgccgccggg atctgggggc aacacattgc cgaatatgcc
      721 gatctgcgca ttcgcatgtt cccggcgaaa ggatcgctgc tgatcatgga tcaccgcatt
      781 aaccagcatg tgatcaaccg ctgccgtaaa ccttccgacg ccgatattct ggtgcctggc
      841 gataccattt cgctgattgg caccacctct ttacgtattg attacaacga gattgacgat
      901 aatcgagtga cggcagaaga ggttgatatt ctgctgcgtg aaggggaaaa actggccccc
      961 gtgatggcga aaacgcgcat tttgcgggcc tattctggcg tgcgcccgct ggttgccagc
     1021 gatgacgacc cgagcggacg taacgtcagc cgtggcatcg tgctgctcga ccatgctgaa
     1081 cgcgatggtc tggacggatt tatcaccatc accggtggca aactgatgac ctatcggctg
     1141 atggctgaat gggctaccga cgccgtgtgc cgcaaactgg gcaacacgcg cccctgtacg
     1201 accgccgatc tggcacttcc tggttcacag gaacccgctg aagttacctt gcgtaaagtc
     1261 atctccctgc ctgccccgct gcgcggttct gcggtttatc gtcatggcga tcgcacgcct
     1321 gcctggctga gcgaaggtcg tctgcaccgt agcctggtat gtgagtgcga agcggtaaca
     1381 gcgggtgaag tgcagtacgc ggtagaaaat ttaaacgtta atagcctgct ggatttacgc
     1441 cgtcgtaccc gtgtggggat gggtacctgc cagggcgaac tctgcgcctg ccgcgctgcc
     1501 ggactgctgc aacgttttaa cgtcacgacg tccgcgcaat ctatcgagca actttccacc
     1561 ttccttaacg aacgctggaa aggcgtgcaa cccatcgcct ggggagatgc actgcgcgaa
     1621 agcgaattta cccgctgggt ttatcaggga ctgtgtggtc tggagaagga gcagaaagat
     1681 gcgctttgat actgtcatta tgggcggcgg cctcgccgga ttactctgtg gcctgcaact
     1741 gcaaaaacac ggcctgcgct gtgccattgt cacgcgtggt caaagcgcgc tgcatttctc
     1801 atccggttcg ctggatttgc tgagccatct gcctgatggt caaccggtga cagacattca
     1861 cagtggactg gaatctttgc gtcagcaggc accagcccat ccttacagtc ttctcgaacc
     1921 acaacgcgtg ctcgatctcg cttgccaggc gcaggcattg atcgctgaaa gcggtgcgca
     1981 attgcagggc agcgtggaac tggcccatca gcgcgttacc ccgctcggca ctttgcgtgc
     2041 aacctggtta agttcgccag aagttcccgt ctggccgcta cccgcgaaga agatatgtgt
     2101 agtggggata agcgggctga tggatttcca ggcgcatctt gcggcggctt cgttgcgcga
     2161 actggatctt tcggttgaaa ccgcagaaat agagctgccg gaactggatg tcctgcgcaa
     2221 taacgccacc gaatttcgcg cggtgaatat cgcccgtttc cttgataatg aagaaaactg
     2281 gccgctgtta cttgatgcgc ttattcctgt cgccaatacc tgcgaaatga tcctgatgcc
     2341 cgcctgcttc ggcctggccg atgacaaact gtggcgttgg ttgaatgaaa aactaccttg
     2401 ttcactgatg cttttgccaa cgctgccgcc ttccgtgctg ggcattcgtc tgcaaaacca
     2461 gttacagcgc cagtttgtgc gccagggtgg cgtgtggatg ccgggcgatg aagtgaaaaa
     2521 agtgacctgt aaaaatggcg tagtgaatga aatctggacc cgcaatcacg ccgatattcc
     2581 gctacgtcca cgtttcgcgg ttctcgccag cggcagtttc tttagtggcg gactggtagc
     2641 ggaacgtaac ggcattcgcg agccgattct cggccttgat gtgctacaaa ccgccacgcg
     2701 gggtgaatgg tataagggcg atttttttgc gccgcaaccg tggcagcagt tcggtgtaac
     2761 cactgatgag acgctacgcc cgtcgcaggc agggcaaacc attgagaacc tgtttgccat
     2821 cggttcggtg ctgggcggat ttgatcccat cgcccaggga tgcggcggcg gtgtttgtgc
     2881 cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc gcaggaggcc aacaatgaac
     2941 gacaccagct tcgaaaactg cattaagtgc accgtctgca ccaccgcctg cccggtgagc
     3001 cgggtgaatc ccggttatcc agggccaaaa caagccgggc cggatggcga gcgtctgcgt
     3061 ttgaaagatg gcgcactgta tgacgaggcg ctgaaatatt gcatcaactg caaacgttgt
     3121 gaagtcgcct gcccgtccga tgtgaagatt ggcgatatta tccagcgcgc gcgggcaaaa
     3181 tatgacacca cgcgcccgtc gctgcgtaat tttgtgttga gtcataccga cctgatgggt
     3241 agcgtttcca cgccgttcgc accaatcgtc aacaccgcta cctcgctgaa accggtgcgg
     3301 cagctgcttg atgcggcgtt aaaaatcgat catcgtcgca cgctaccgaa atactccttc
     3361 ggcacgttcc gtcgctggta tcgcagcata gcggctcagc aagcacaata taaagaccag
     3421 gtcgctttct ttcacggctg cttcgttaac tacaaccatc cgcagttagg taaagattta
     3481 attaaagtgc tcaacgcaat gggtatcggt gtacaactgc tcagcaaaga aaaatgctgc
     3541 ggcgtaccgc taatcgccaa cggctttacc gataaagcac gcaaacaggc aattacgaat
     3601 gtagagtcga tccgcgaagc tgtgggagta aaaggcattc cggtgattgc cacctcctca
     3661 acctgtacat ttgccctgcg cgacgaatac ccggaagtgc tgaatgtcga caacaaaggc
     3721 ttgcgcgatc atatcgaact ggcaacccgc tggctgtggc gcaagctgga cgaaggcaaa
     3781 acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc acactccgtg ccatatggaa
     3841 aaaatgggct ggacgctcta caccctggag ctgttgcgta aaatcccggg gcttgagtta
     3901 acggtgctgg attcccagtg ctgcggtatt gcgggtactt acggtttcaa aaaagagaac
     3961 taccccacct cacaagccat cggcgcacca ctgttccgcc agatagaaga aagcggcgca
     4021 gatctggtgg tcaccgactg cgaaacctgt aaatggcaga ttgagatgtc cacaagtctt
     4081 cgctgcgaac atccgattac gctactggcc caggcgctgg cttaaactcc tttctgatgc
     4141 ccggtaagca tgtggttacc gggcattttt gcgtacacga ttccgtgccc aatgtatgcg
     4201 ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa ggctgaaact ggcagatgtc
     4261 aaaggcctgg gataaccgta atgtcgcgtc atcataaata tcaggtgacg gacaaccatg
     4321 accgaatcaa caacctcctc cccgcatgat gcggtattta aaacctttat gttcacaccc
     4381 gaaaccgcac gggattttct cgaaatacat ttgccagaac cactgcgcaa gctttgcaac
     4441 ctgcaaacct tacgcctgga acccactagt tttattgaaa aaagtttacg cgcttactac
     4501 tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg gctatatcta ctgcgtgatt
     4561 gaacatcaaa gctctgcaga aaagaatatg gcttttcggc taatgcgcta tgccactgcc
     4621 gccatgcagc gtcacctgga taaaggctat gacagagttc cgctggtggt gccattgctg
     4681 ttttatcatg gcgaaacatc gccctacccg tactcactta actggctgga tgagtttgac
     4741 gatccgcaac ttgcccggca gttgtacacc gaagcttttc cgttggtgga tatcaccatc
     4801 gtacctgacg atgagatcat gcaacatcgg cgtatagctc tgcttgaact gattcaaaag
     4861 catattcgcg accgcgattt aatcggtatg gtcgacagga tcaccacgct tttggttaaa
     4921 ggcttcacta atgacagcca gctacaaaca ctgtttaatt atctgctgca atgcggcgat
     4981 acctcccgtt tcacccgttt tattgaggag atttccgaac gttcaccact acaaaaggag
     5041 agattgatga ctattgctga gcggctacgg caggaaggac accaaattgg ctggcaggaa
     5101 ggtatgcatg aacaagccat taaaattgct ttgcgcatgc tggaacaggg cattgatcgt
     5161 gaccaggtgc tcgcggccac ccagctaagc gaagccgatc tggcagcgaa taaccactaa
     5221 ttaacacagg cccacagccg atccccatgg gcctttgata tcaataacta ccttttatgc
     5281 gtggcccatt tttgcctgat ttaaacatcg ccagtcgttg atccagggca tcgctgtaga
     5341 gcatcgtgtc aacgccaaca gcgacaaagt tcgctcccca cgccaggcat tgctgcgcca
     5401 tatcaggagc cacagccaga aaaccagccg ctttacccgc agcacggatc cgccgaatgc
     5461 tggtttcaat aattcgctgc acttccgggt gcccggcgtt atccgggtag cccaacgacg
     5521 cagaaagatc cgctggtcca ataaacacgc catcaatccc ttcgacgtcg aggatctcgt
     5581 ccaggttatc cagtgccgtt ttactttcca cctgcactaa cagacaaagc gaatcgttga
     5641 cttgcgccat gtaattctca atgcgtcccc agcgcgcagc ccgtgccaca ctggccccaa
     5701 caccacgctc accgtaggga ggatagcgcg tggcagacgc cacctgacga gcctgatcgg
     5761 cagtatcgac catcgggatc aatagagttt gcgcgccaat atccagaact tgtttaatca
     5821 gcgatttact gccttccacc ggacggatca cgggttggct ggcatagggc gctaccgcct
     5881 gtagctggtg ataaagatcc tgaatggtgt ttggcgcgtg ctccccgtca atcagcaacc
     5941 agtcataacc ggaagtagcg gcaatttctg ccatataaga agtagtcgag cttaaccaca
     6001 gaccaatttg cacttcgccc ttgcgtaaac gttctttaaa gggattggtt aataatgcgt
     6061 tcatcgtgtt ccttaaccta tttaatgatg tgccacgtcg gtctgcgcaa cagtacgatt
     6121 tacacgcaga gtgaaaataa tcagcgaacc gatgaccgcc accgctgcca gtgtcagtaa
     6181 tcctgccgca tcgctggcaa ataacgtttc tgctttcacg cgcaggatcg gggcaataaa
     6241 cccgcccact gcaccaaaca ggtttacaaa accaatcccc gcagccagcg ccgtaccgga
     6301 aagcagttgt gtcggcatcg tccagaacac cggctgcacg gcaataaacc cgatagctgc
     6361 aacacacagc gctacgatcg ccattactgg agaaagcagc ccggacagac caataccaat
     6421 gcccgccgcc agtaatgtca gcgctgcgac attacgccgt tcgccggttt tatcggaata
     6481 gcgcggaata agccaggtcc caaacaaggc cgcaacccac ggaatggcgg tgaccaccga
     6541 cgctgtaaag cccacttttg ttcccagcaa tgccgcaacc tgggtcggca ggaagaaaat
     6601 taatccgtaa accgccacct gaatggtcag gtagataatc gctagttgcc agactcggcc
     6661 attacgcagc gcatcgctca gccgagaagt cactttctgt tgttcttcac ttgccagttg
     6721 attgataagc aacgtttttt cttgtttact cagaaaacgt gcctgctccg gtgtgtcatc
     6781 aagccaaaag aatgtgaata ccccagcacc gactgccaac aatccttcaa tcacaaacat
     6841 ccagaaccag ccgggatgcc ccataaatcc atgcatctcc aacagcgcgc cggaaagcgg
     6901 tgatcccagt gttaacgcca gcggtgcgcc catatagaac agccccataa tgctggcgcg
     6961 attacgctgc ggaaaccatt gcgaggtgag ataaatcata ccagggaaaa atccggcctc
     7021 cgcagcacca agcagagtgc gaactatcag aaatttcgct tcagtatcgg cccatgccat
     7081 ggctgccgaa agaaatcccc acagcagtgt tgtcgtacca atccaggttc tggcccccag
     7141 tttgcgcatc aaaagattcg ccggaacacc cagaaacgca tataccacaa agaaaatgcc
     7201 tgctcccagc gcataagctt cattactcaa cccggtatca atctggtagg tctgtttggc
     7261 aaaaccgata ttcgaacggt cgagaaacgc cagcacatac agcgccaaca taaacggaat
     7321 taaacgcaca cggtttttct tcaccacggc gtcaagcaaa gcggtgctca taaaaagctc
     7381 cttagaatat gtgggcgtta ccgttgtcac agcaacgccc ggagtgatta gtggctgtag
     7441 gggcgtttca aattgcagtc acgattgagt tcgacgccaa aaccgggttt atcaagcact
     7501 gatttatgaa tacgaccatt caccggaacc ggctcattga gcagaatcgg gtcaaactgc
     7561 ggacgcatcg ttgaacaatc cgggctggtc atcaggaatt cgctgaatgg cgtattggtg
     7621 aaggtgatca ccgcatggtg agagtaaaca gacgaaccgt gcggcaccac cagttgcccc
     7681 ctggatttgg cgattgcggc aatttccacc agcgtggtta aaccaccgca ccagccaaca
     7741 tccggctgca taatgtcgat accggtttct gaaagcgtac gaaaagattg cagtgtgccg
     7801 tggtgctcac cgctggtgac catcattccg actggcgcgt tgcgtttcag ttcgcgataa
     7861 ccttcatact gctgtggcgg caggcactct tcgatccatt tcaggttata gggcgcgcaa
     7921 gcgtgggcca gtttggtggc atagttcacg tcctgactca tccagcagtc gagcattaac
     7981 cagaaatcct caccgcattt ttcacgcata tccgcgacca taacggcatc tttgcggatc
     8041 cccgcatcgc catcatgtgg cccccagtgc gtcggcattt tgccaccgat aaagcccatc
     8101 tcttttgcca gatccggacg cgcgcctgtg gcgtagaact gaatctcatc acgaactgcg
     8161 ccgcctaaaa gtttataaac cggaagcccg accactttgc cgaacagatc ccacagagcc
     8221 agatcgacac aggaaatcgt gttcatcacc aggccaccag agccggagta atacagggtg
     8281 gcattgagca tttgatcatg gatcagtttg atatcactga cacatttacc ctcaatgaaa
     8341 cggttaagat gtttttcgac aataaaacag cccatttcac cggctgtcga aacagcgaat
     8401 ccggtctggc cgttttctgc ttcgacttca acaatcaacg tgccaagaac gttaatgcca
     8461 aatgactggc gtgactgctc gtaatcgcgg tatttactca tcggggtggc aatatgatcg
     8521 tcaatccagt gattcgcccc ctggtcgtga taatcaccgc cgccagcacc tttttctgct
     8581 gtcgcaccgc cagtaaacca ggcgcgaacc tgtttgattt ttggtagggt catgatgttc
     8641 tccattgtta tgaggcctgt aagtcaaagg gacttttcca tcccaacaga cgtgaaatat
     8701 ccctggcgca ggcaatggcc ttgcccgcca gataatcacg gtattcttca ttgatttgta
     8761 agcgggtacc gaccaccgag atcgcagcgg taagctcgtt attggcgtta aacaccggcg
     8821 cagcgacaca acggacatcg gggtaatctt cgccgttgtc atagctccag ccctgacggc
     8881 gaatacgcgc cagttcttcg tgaagttgct gtggatgagt aatcgttgtg ggtgtcgcct
     8941 gctcccagac cagcccttcg ataatacttt gctgtaccgc tgcaggttgc caggcaagca
     9001 ggcatttacc tattccggag cgataaagcg aaaggctttt tccttcatgg gaacgcacgc
     9061 tgatagtggc tgatgactcc acttttaaaa tgtaataagc gctgccgtta tcaataatcc
     9121 ccaggtgaca taacagcccg gtggtatcca tcagttgcgt caaacgcggt cgagccagtt
     9181 cccggagatc catcttgctt aacgcatgac cagaaagctc caccagcctg gtccacaaac
     9241 aaaaattttc ctggtgatca aggctaagaa aacgctgacg cctgagttca ttaagcagca
     9301 aataggccgt gcttttaggg attcccaatg tgtcaatgat cgtcgcagca ctacaggggc
     9361 caatgcgggc aatcaaattg agaatatcaa tagcccgtgt cagggcggga actttgcttg
     9421 attccaacat actggactcc agtcttagat actggaatca gtctcgttgc tcagggggag
     9481 taaaattgtg aagcgcatca aatcccgctc ctcgtccagc ggactggaga aattacgcac
     9541 aaatttgtga atcagtgcac ggattttttc ttattgagta caacaggttg gacaagtttt
     9601 gccggagggg gaatgtgccg gtgcagtaaa ccggcacaga gtggcggaat cagacagata
     9661 aggtcatgga ctcaacaacc tcaatccagc catgctcact ggcgatatcc tggccgttta
     9721 accaacggcg cagcatattc agtgccatca ttgcgcacac ttcctgacgg atagccaggc
     9781 tgtagcgtgt agtgctgaaa cgcacacgca gagcgaaagt gccgtctggc gtggctagcg
     9841 caaagttgag atgctcgttc tcgaaacccg aaacagccag tgccagcccg gcaaaatggt
     9901 tggcccgccg ttctgtaatc cagtgcgcag tttgcgccag ggtttcttcc tgtgaaggaa
     9961 ccacttcaca cgccagcaat ggagcacctg cgcgagaaag ttgcaaagcc aataaaccac
    10021 cggtgaattg ctcgctcaac gtcaggctga actggcggtt ttgcaattcg cgactgatct
    10081 gcgcgggcag tccttcggtg ccttcgaaaa tcacgctctg tccggcaaca cgtttaacgt
    10141 ccagccacag tttttccatc gcctgttgct cgcttgccgg tccggtgagt ttcagttcga
    10201 tgatcggcat tgaggagcga tagcccattg ttacacccgg cggcagttgt agagtgtcca
    10261 ggctttgtgc cagatcgctt tccgaacgac caaaggtagt taaacgcaga caaaccggcg
    10321 actgtggtaa agaaaaacgc tcgcgcaggc gcggcaggat ttcgtgctcg accatcacct
    10381 taaattctga cggtacgccg ggagtaaaga acatcaggca acgattaagc cgcacggcaa
    10441 aaccacaggc ggtgcctacc gggttattga taaattcagc actggcaggc agctccgctt
    10501 gtttacggtt gctcggtgcc attactcgtc cacgttcgtg gaaatagcgt tccatctctt
    10561 tgagccaggc ttcatgcagc accaggcctt caccttttgc tgtcgcagcg gcgagtgcac
    10621 ttaaatcatc actggtcggt cccagcccgc cgttaacgat cagcacatcg gcgtgctgac
    10681 tacgttcgcg aagaatggtg actaagtcat caaggttatc ccccaccgta ttgcggcgag
    10741 ataatggcaa cccctgatga aagaaaaaat cggccagcca ggcagcgtta gtgtcaacga
    10801 tttgcccgtg taacacttca tccccggtgg ataacatttc cacttttaac atggttatct
    10861 cctgcttttg ttatcgaatc actataacgc aaagggagat aaggcgtagt aagaaactga
    10921 atggcgcgac agaatgccgc gccggaaggg atcagaaact ggcgttaacc ccaacatacg
    10981 ggccgtcagc cacggcgttg tcgcggttac cgtctttacc cgacagattc aggtagcgat
    11041 aacccgcctc aatactgact ggatgcataa tggtgtaacg cgcgccagca ttcgcttcct
    11101 cataactttt aataccgctg gagagtgaat ccggagagta gtaatactcg ccaaacaaac
    11161 ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca taaccttcat
    11221 cgccgtaatt cgggttggtg taaacgcctt ttccgccaac ggtcgccatt aacggcccga
    11281 gaggcagatt caaccccagc cccacgccag cgacgtcacc gtcgtcgtcg ttatgtgtcc
    11341 agttaccgct taacgccagg cccgtcgatt cagtcccaaa accgacacca atattggtgt
    11401 attctttacc cgcctgaccg ctgatgctca ttgcattgac cgatgcagaa accaacagca
    11461 ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca agcaattaat
    11521 caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat gagcgaaaaa
    11581 agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc cgaaaaaata
    11641 aacacacttt accgatacac acaaaaataa taataagtgc agttatcatc gcaaaagaga
    11701 ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt tttatttata
    11761 aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct
    11821 tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg
    11881 ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa
    11941 gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt
    12001 cgcaccaaga cgtatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt
    12061 gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg
    12121 gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt
    12181 ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag
    12241 tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca
    12301 tccagataat caggtttaaa tgtcgcatca cgcttatttt tagcaatata tgtcaggcaa
    12361 tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt
    12421 gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag ttttttaccc
    12481 gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa
    12541 tcagggatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc
    12601 gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg
    12661 cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata
    12721 cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc
    12781 gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg
    12841 taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag cgacatttct
    12901 ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa
    12961 aattttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct
    13021 aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca
    13081 aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga
    13141 aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct
    13201 gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt
    13261 gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc
    13321 gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg
    13381 ccttccctga cctgggtttc aaccctcaat atgatttcct tgttgggtgc aacgccggta
    13441 atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga gtcagccatt
    13501 acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc agatattgac
    13561 gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc
    13621 gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt
    13681 catgccatta aaaatattac ctgtgcggaa ggtggcctga ttgtaactga taatgaaaac
    13741 cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga
    13801 caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg
    13861 accgatatta acgccgcgat tgccctgaca cagttagcaa aattagagca cctcaacacc
    13921 cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca
    13981 ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat
    14041 gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt
    14101 ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc
    14161 acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcttt gccgctgttc
    14221 ccggatatga ctaccgccga tgccgaccgt gtcatcacag cccttcagca actcgcagga
    14281 caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat
    14341 aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg
    14401 gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc cgcgcatatg
    14461 ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat
    14521 tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga cttaattatt
    14581 acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc
    14641 gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt
    14701 aaaacagctt cgaagatgat taacaggctt attcagcgca ccaccggcaa agcgatgggt
    14761 gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc
    14821 catgaacgta gcacctttat cccgattctg gcgaatatct tcgcccgccg tgccattgaa
    14881 attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg
    14941 attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt
    15001 ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta
    15061 cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg
    15121 tttactttta ttggcgctca gtttatcggc atgggattgc tcggtgaata tatcggcagg
    15181 atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc
    15241 agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg
    15301 gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc cataccgata
    15361 atcccgctga aaaagccttt tatggttcgg tggctcgtct ggcggcggaa agaggcattc
    15421 cagtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt gcccaactct
    15481 caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag attttgcagc
    15541 tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg
    15601 cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa
    15661 tggtaaaacg tgccgatgct ggggccattg tggcgcaact gcgcgttgcc attgcgccag
    15721 acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag ctactggaac
    15781 agacattacc cgccattaaa cacggtaata ttctggaaat tgcccagcgc gaaaacgaag
    15841 ccacctgttt tggtcacaga acgccagatg acagtttcct tgaatggcat aaaccggcat
    15901 ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg
    15961 ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac
    16021 agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg
    16081 aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga
    16141 tgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc
    16201 gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc
    16261 tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagcgat gcgataagcc
    16321 gttttctgaa tcatccgcat tttcactttg tcgaaggcga tatcagtatt cattccgaat
    16381 ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc
    16441 cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc
    16501 gcattatccg ctactgcgtt aagtaccgta agcgaatcat cttcccgtca acttcagaag
    16561 tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc
    16621 cggtgaataa accacgctgg atttattcgg tgtcaaaaca attacttgat cgggtgatct
    16681 gggcctatgg cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg
    16741 gaccacgact ggataacctt aatgcagcgc gaattggcag ctcccgcgct attacgcaac
    16801 tcattctcaa tctggtagaa ggttcaccaa ttaagctaat tgatggcgga aaacaaaaac
    16861 gctgctttac tgatatccgg gacggtatcg aggcgttgta ccgcattatc gaaaatgcgg
    16921 gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg
    16981 aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcacttcc
    17041 caccgtttgc gggctttcgt gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg
    17101 acgtagagca tcgtaaaccg agcatccgca atgcccgccg ctgcctggac tgggaaccga
    17161 aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc
    17221 ttacggataa accatcatga ccaaagtcgg cttacgcatt gatgtcgata cctttcgtgg
    17281 cacccgtgaa ggcgtgccac gtctgctgga aatcttgagt aagcataata ttcaggccag
    17341 catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc
    17401 acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct gggatatttt
    17461 actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg
    17521 tgaagcggct aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc
    17581 ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac
    17641 tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga
    17701 ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg
    17761 ggccatgccg ttccgtccac tgctcgaatc aggaaaccct ggcactgcgc aaattccggt
    17821 gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg
    17881 ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga
    17941 agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga
    18001 aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt
    18061 tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag
    18121 tcgctgatga aatcggtacg ttaccttatc ggcctcttcg cgtttattgc ctgctattac
    18181 ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc
    18241 agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat
    18301 ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg
    18361 aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg
    18421 acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt
    18481 tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt
    18541 gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg
    18601 aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc
    18661 aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa
    18721 aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg
    18781 gttctcccct ggggactggc gatagcgcag cgggagcctg acttctggca ctattttttc
    18841 tgggttgagc atattcaacg ctttgcactg gatgatgccc aacatagagc tccgttctgg
    18901 tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca
    18961 ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg
    19021 ataatgccgc tgctgttttt ctccgtcgct aaaggtaaat tgcccaccta tattctttcc
    19081 tgctttgcat ctctggcaat gctgatggcg cattacgctt tgctggcagc aaaaaataat
    19141 cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcttcac tggcattatt
    19201 gccacatttg tggtctcccc gtggggacca atgaacacgc cggtgtggca aaccttcgag
    19261 agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac
    19321 accttaacaa acgtcgaaaa gacctggtct tttgccgcgc tttgcccgct ggggctggcg
    19381 ttgctggtag gattttcaat tcctgacaga gtcatggaag gaaaacatcc gcaatttttt
    19441 gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt
    19501 gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca
    19561 ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat
    19621 gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattacact cgtgctttcg
    19681 gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt
    19741 caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg
    19801 ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga
    19861 taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc
    19921 ttgccatggt gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga
    19981 tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg
    20041 tatcgccgcg tcactggtgt ggggtggcgt tcattattgg cggcattgtg atcctcggga
    20101 gtacggtgta atgggcctga tgtggggatt attcagcgtc attattgcct cagttgcgca
    20161 attaagcctg ggttttgcgg cgagtcatct gccgccgatg acgcacttat gggattttat
    20221 cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta
    20281 tctgctttcc gttttttgtt ggtataaaac gctacataaa cttgcgctaa gcaaagccta
    20341 tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg
    20401 ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat
    20461 gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgcca ctttacactg
    20521 ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt
    20581 ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac
    20641 ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat
    20701 aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgaccagcc attccattgc
    20761 attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata aacctattat
    20821 caacggcaat ggaatggcaa aattgcgaag tgaaactaat gatttcttaa tcctggcgtt
    20881 gcacccactc ttttaacgcc tgacgtgaaa ttttaatacc gccgtttttc agctccggcg
    20941 gtaaagttag ccagcgcacc ggttgttgaa aacgagccag cttatctttc acccattcgc
    21001 caagatcaac ggtttgctgg tcatactcca ccaccgctac cggtcgatga ccaaactcct
    21061 tgtcggcaac ggggacgata aacacctgca aaaccgcagg atgtgcagca attacgcgct
    21121 cgacttcttc cggctgaata ccctctccgc cactgaagaa cagattgtct aaacgtccga
    21181 caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt
    21241 cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata ctggcagccc
    21301 gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc gaaccaacgt
    21361 ctgccaggcc gtcggcttct ttcgcacaca ccgtggaggc aaactcggtc agaccatagc
    21421 cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc gggatagccg
    21481 cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc aaacgccaga
    21541 gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc aatggctgtt
    21601 tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt ccctgaccgg
    21661 agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac ggaatcagcg
    21721 ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg gctttcggca
    21781 aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc cacgtagcgg
    21841 catgtgcgcc ttcaacccgc tgaatgtgca gcgacgttaa tgccggaaac gtgttttccc
    21901 catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca agcaacggtt
    21961 gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac gccagccagg
    22021 cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc tcaaccacac
    22081 cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg cgccagttga
    22141 gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc cagtgacgcc
    22201 acggccagtc agagaagatc ataacaactg ctccagtaca tcagcgtcca ctaacggcag
    22261 tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg tgtccagtcc
    22321 cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta gacctaagct
    22381 cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg cctgtacctg
    22441 ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc
    22501 ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg caatggcaat
    22561 gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa
    22621 cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac ctttcagcgg
    22681 tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct ccagcaacag
    22741 attaaccacc atgccgtcgc gcacggcttc gtacaatccc actttgacct tcgccacttt
    22801 ctcgcctggc atatcagcaa gtttgaggat cagatcgtca ggatcgccat tacacagtgg
    22861 tgccgcacgg taattggccg cctgcgggag cgtatctgcc agctccgcca atgcacagct
    22921 tacgccaaag gcaacggaag gcatctgcgg tagctcgcca tcgcctgcca gccagttatt
    22981 tacccaagca agcagcacaa tttgcgcctc ttcccaggtt tcctgactga agcccggcag
    23041 tggggagatc tccccccacc cttcgcgctc gccttcacgc aggcaaacat acagcccgtc
    23101 gcgggttttt aaccgcctgt cgcgcagaac catccccgcg tccatgggga tctgccagcg
    23161 gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc aggctgacgt
    23221 ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa cagcatggtg
    23281 gcgttgcccg ccagctcctg cagacccgcc tgaccgtcac agtcggcgtt cagcgcagct
    23341 ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca acggacggtt
    23401 tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt
    23461 tttgcatcgt actgacggca caggaaccag atttcacgcg cttttttctg tccgacgatg
    23521 cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg gccagtctga
    23581 ccgaagatgg cattatctgc cgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca
    23641 ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt acggatctga
    23701 cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata gccgccgtaa
    23761 tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc gccagtcaga
    23821 atgatcacgc cgacgttgtc gtcataacgc gcatctgcca gtgcctggat catctctttt
    23881 accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat ttttgcgata
    23941 ccgtcggtgg atttttcata acgaatgtcc tcaaaacctt cggagcagtc gtgccattca
    24001 accggtgcgt aaagcattgc ttcatcagga taaatcatat tgtgtccttt agtcagaaac
    24061 gcaagatctg cgccagactt gcgataacgc cagcgggatt ttcccgatgc gcgttatgtc
    24121 cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccaaggcg cggaatttgc
    24181 tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg ttagcacgta
    24241 aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt gcgccattat
    24301 tgttactgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag gcaaaaacag
    24361 gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa cgctgcgccc
    24421 attggcgatc ggaacgctga cgttccgcgc gttgttcagc attttgcaac ccaggatgcc
    24481 cgccttcgac aacaacccca caaagccccg gcatcccctg gcaagccgcc atcatcgcca
    24541 cccgtccccc aagcgagtac cccaccaacc agaagttaag gatgttgtaa ctaaccaggg
    24601 ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac
    24661 cgtgacctgg gagataaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt
    24721 gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt
    24781 tgccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gaagttgctg
    24841 gagcgtttgc gcaccatcgg tgtcgttaac caccatttca attaccgtgg tggttggcgt
    24901 gcgccaggca tcggcaagtg ccgtttcaag ttcctgccag ttttgcggac gatgatattt
    24961 cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg
    25021 ctcgcgctcg cttttcggcg ttggcagcag cgagaaaatt tgcccgccgt tgttgttcac
    25081 cacaattaat atcagcggcg cggaaacctg acgcaataac gccagcgcgt tgagatcgta
    25141 aagtgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc
    25201 ggcggcagtc gagagcagcc cgtcgatacc gctggcccca cggttgctgt acaccgggta
    25261 acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccgacaaa
    25321 caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa
    25381 cgcatcgcga cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac
    25441 gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa tattggcaat
    25501 taagcggcgt ccacgatggt gcgccggatc aagccgccct tcgatgtcat ccacaatcca
    25561 gtactcttcg ggttcacagc ttgcctgcca ttgcaggagc cgtttgcccg tcaggctgct
    25621 tcccagttgc accacaattt gcgcctgttg cagttcgctg gtcgctttgg cattgcctaa
    25681 ccagagatcg gcacacggca gcggctgtcc ggtttgcgac agcacgtcgc caatcagcgg
    25741 ccagccaaga gtttgcgccc acagtgcaac ttttttgccc tcttccgcac tcatgcgccc
    25801 ggcaaccacc acgccgcgtt tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc
    25861 cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcgc ccagccgctg
    25921 ttgccagcta agcccggtgt cgtccatttc gccatacagc ggttcggcaa acgggcagtt
    25981 gatatggact ccaccagaat gaagcgtacc gagagcatgg tcgatggtag aaaccagcca
    26041 acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc
    26101 gaacattccc ggctgacgaa tcgcctgatt cgcgccgcag tcaatcagct ccggcgggcg
    26161 atcggcggtt aacaggatca gtttttctcc ggttaaccca gcttcaatca gtgccggata
    26221 gagatttgct accgccgtac cggaggtcac aatcaccgcc accggctgct tgctgacttt
    26281 cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt ggtgaatgaa
    26341 tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagccag gggcgataca
    26401 gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt
    26461 aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct
    26521 acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat tccatctgta
    26581 ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc tcggggtcgg
    26641 aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta atttttgctg
    26701 agcgcaggga aacgcagaat tcgctttgct gtaatgagag atagcccgca gaaccggcgt
    26761 accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta
    26821 agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatta tccgctttgt
    26881 tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg
    26941 gcggtaaaac atccagcgtc tgggtatcgg cctgtaaccg ttggcagata tcttccacca
    27001 ccagcatatt ctcgcgctgg tttttatcat ccgccatcag ccactcacct aactgctgcg
    27061 cctgcttatc atcaggatga tttgctaccg ttcccgccag cgcttcagta cgcagcgctt
    27121 tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt tcgccatcaa
    27181 aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg
    27241 ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc actttgtcga
    27301 gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt
    27361 ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc ttgatactca
    27421 ccagtgtggc gataaattct cttgccttaa tcgcatcgtg ctgaagggag ctttcgctga
    27481 atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg cgtggtaaaa
    27541 gtaaattgcc ctgcgacggg tcaaacgcat tcagccccca aatgcgtaag tcggcgtgtt
    27601 ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc
    27661 ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc gggtatgttt
    27721 gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga
    27781 taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca
    27841 gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg
    27901 gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca
    27961 gtcctagtac cagcccaacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga
    28021 cgtagtcatc agcacgataa actgcctgct tcgcccgata gtaataactg tctgaagcct
    28081 ggctaacccg ttttttcaca tcatccagcg ctttttctgc acgcgctttc agctcaacat
    28141 atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca
    28201 gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc gttctccatt
    28261 tgcgtaaaac ctgtcactaa ctatagacaa gggttgttga ttacgcctga attacctcgc
    28321 gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca gggataaaac
    28381 cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag
    28441 gccagtgatg cgtgcaggtg tcgagggttt tactcatcag ttgctgcccc actttttcgc
    28501 cacgcaacgc ttcactgaca atcacccgac caataacgac cggctcaaga tcatcatcac
    28561 ttttcagaat cctcgcatat gccaccagtt catcattttt ccaaccgaga atatggcgat
    28621 tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc tcgacaacaa
    28681 acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat tcagagtgat
    28741 gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta tactagccgg
    28801 aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact tcagccggtg
    28861 tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga
    28921 gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct
    28981 ttaaccctgg gaaactggac aagattttta tcagtcacct tcatggcgat catctctttg
    29041 gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt
    29101 atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga
    29161 ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc
    29221 gcaaagtgac cgcttatccg atggaacacc cactggaatg ttatggctat cgtattgaag
    29281 aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg
    29341 gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat ggaaggcaga
    29401 ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg
    29461 ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg
    29521 aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac
    29581 gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt acccacgtca
    29641 gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg tcaattttcc
    29701 cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaa
//
LOCUS       DAZPLM010000029        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPLM010000029 DAZPLM010000000
VERSION     DAZPLM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.029"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHVC7_001106"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHVC7_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR2115214.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHVC7_001107"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHVC7_001107"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR2115215.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHVC7_001108"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHVC7_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR2115216.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHVC7_001109"
     CDS             complement(3644..4030)
                     /locus_tag="ACHVC7_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR2115217.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHVC7_001110"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHVC7_001110"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR2115218.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHVC7_001111"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHVC7_001111"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR2115219.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHVC7_001112"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHVC7_001112"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR2115220.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHVC7_001113"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHVC7_001113"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR2115221.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHVC7_001114"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHVC7_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR2115222.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHVC7_001115"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHVC7_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR2115223.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHVC7_001116"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHVC7_001116"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR2115224.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHVC7_001117"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHVC7_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR2115225.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHVC7_001118"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHVC7_001118"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR2115226.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHVC7_001119"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHVC7_001119"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR2115227.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHVC7_001120"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHVC7_001120"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR2115228.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHVC7_001121"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHVC7_001121"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR2115229.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHVC7_001122"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHVC7_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR2115230.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHVC7_001123"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHVC7_001123"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR2115231.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHVC7_001124"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHVC7_001124"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR2115232.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHVC7_001125"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHVC7_001125"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR2115233.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHVC7_001126"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHVC7_001126"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR2115234.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHVC7_001127"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHVC7_001127"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR2115235.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHVC7_001128"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHVC7_001128"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR2115236.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHVC7_001129"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHVC7_001129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR2115237.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHVC7_001130"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHVC7_001130"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR2115238.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7121 c         8159 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccaaaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPLM010000030        29509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPLM010000030 DAZPLM010000000
VERSION     DAZPLM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.030"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..645)
                     /locus_tag="ACHVC7_001131"
     CDS             complement(<1..645)
                     /locus_tag="ACHVC7_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR2115239.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLL"
     gene            complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHVC7_001132"
     CDS             complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHVC7_001132"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR2115240.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHVC7_001133"
     CDS             complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHVC7_001133"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR2115241.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHVC7_001134"
     CDS             complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHVC7_001134"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR2115242.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHVC7_001135"
     CDS             complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHVC7_001135"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR2115243.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHVC7_001136"
     CDS             complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHVC7_001136"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR2115244.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7182..8417
                     /gene="moeA"
                     /locus_tag="ACHVC7_001137"
     CDS             7182..8417
                     /gene="moeA"
                     /locus_tag="ACHVC7_001137"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR2115245.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8417..9166
                     /gene="moeB"
                     /locus_tag="ACHVC7_001138"
     CDS             8417..9166
                     /gene="moeB"
                     /locus_tag="ACHVC7_001138"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR2115246.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHVC7_001139"
     CDS             complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHVC7_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR2115247.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10138..11037
                     /locus_tag="ACHVC7_001140"
     CDS             10138..11037
                     /locus_tag="ACHVC7_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR2115248.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11043..13475
                     /locus_tag="ACHVC7_001141"
     CDS             11043..13475
                     /locus_tag="ACHVC7_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR2115249.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHVC7_001142"
     CDS             13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHVC7_001142"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR2115250.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14588..15853
                     /locus_tag="ACHVC7_001143"
     CDS             14588..15853
                     /locus_tag="ACHVC7_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR2115251.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15820..16980)
                     /locus_tag="ACHVC7_001144"
     CDS             complement(15820..16980)
                     /locus_tag="ACHVC7_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2115252.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17218..18519
                     /locus_tag="ACHVC7_001145"
     CDS             17218..18519
                     /locus_tag="ACHVC7_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115253.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18534..20897
                     /locus_tag="ACHVC7_001146"
     CDS             18534..20897
                     /locus_tag="ACHVC7_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR2115254.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21077..22672)
                     /locus_tag="ACHVC7_001147"
     CDS             complement(21077..22672)
                     /locus_tag="ACHVC7_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR2115255.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHVC7_001148"
     CDS             22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHVC7_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2115256.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23870..24988)
                     /locus_tag="ACHVC7_001149"
     CDS             complement(23870..24988)
                     /locus_tag="ACHVC7_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR2115257.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHVC7_001150"
     CDS             complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHVC7_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR2115258.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25638..25766
                     /gene="mntS"
                     /locus_tag="ACHVC7_001151"
     CDS             25638..25766
                     /gene="mntS"
                     /locus_tag="ACHVC7_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR2115259.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26038..27621
                     /gene="opgE"
                     /locus_tag="ACHVC7_001152"
     CDS             26038..27621
                     /gene="opgE"
                     /locus_tag="ACHVC7_001152"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR2115260.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHVC7_001153"
     CDS             complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHVC7_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR2115261.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28238..28303)
                     /locus_tag="ACHVC7_001154"
     CDS             complement(28238..28303)
                     /locus_tag="ACHVC7_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115262.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHVC7_001155"
     CDS             28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHVC7_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR2115263.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7151 a         8081 c         7393 g         6884 t
ORIGIN      
        1 taacaaatcg tgatctttgc cgttgatacg tagcgtaaaa gagatacatt ttttacccgg
       61 acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg aagccaccga
      121 ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag
      181 cggtccattt acttgttcct gatgataaac aacgatttta tcattcgccc tccgcgaaaa
      241 gagataatat ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc
      301 cattttcaga atcacggcgc gttgaatctc ctctgtattt tttaataaat acagtgagtc
      361 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
      421 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
      481 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      541 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg
      601 ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac
      661 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc
      721 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
      781 accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac
      841 agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc
      901 tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
      961 ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg
     1021 acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
     1081 ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat
     1141 ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
     1201 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca
     1261 ccagccttca taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa
     1321 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat
     1381 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
     1441 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
     1501 cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
     1561 tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt
     1621 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
     1681 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
     1741 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
     1801 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag
     1861 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg
     1921 aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
     1981 aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg
     2041 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
     2101 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
     2161 atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg
     2221 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
     2281 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
     2341 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
     2401 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgagc
     2461 ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc
     2521 gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt
     2581 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
     2641 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
     2701 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
     2761 ccgccggatc attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt
     2821 aaaatgcggt attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg
     2881 cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
     2941 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct
     3001 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
     3061 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
     3121 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
     3181 cgatacttgg cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca
     3241 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg
     3301 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
     3361 cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact
     3421 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
     3481 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
     3541 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
     3601 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
     3661 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
     3721 cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg
     3781 gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct
     3841 ggaatttaat gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat
     3901 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
     3961 aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga tcgagcgtgg
     4021 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
     4081 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
     4141 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
     4201 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
     4261 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
     4321 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
     4381 cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
     4441 cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
     4501 agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct
     4561 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
     4621 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
     4681 cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca
     4741 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
     4801 tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta
     4861 tcgattctct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
     4921 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
     4981 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
     5041 aaaccgctgc gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca
     5101 tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc
     5161 aacgggaaac gtcggggata atccaaccct ttcatcgcac caagttgcgg aacagcagct
     5221 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt
     5281 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
     5341 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
     5401 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc
     5461 accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
     5521 agttgatgcg gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc
     5581 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
     5641 tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat
     5701 atcatcgcca tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt
     5761 tcaatcactt cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc
     5821 gcctgttcca acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg
     5881 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
     5941 attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg
     6001 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
     6061 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
     6121 gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
     6181 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
     6241 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccga tacaagaaac
     6301 cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg
     6361 tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa
     6421 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
     6481 atggtcgagg acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg
     6541 cgtggagaaa atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc
     6601 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
     6661 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
     6721 cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt
     6781 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc
     6841 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg
     6901 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
     6961 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
     7021 tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
     7081 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
     7141 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
     7201 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
     7261 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
     7321 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
     7381 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc
     7441 atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag
     7501 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
     7561 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
     7621 cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac
     7681 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
     7741 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc
     7801 tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc
     7861 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
     7921 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
     7981 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
     8041 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     8101 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     8161 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     8221 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     8281 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     8341 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     8401 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     8461 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     8521 gatcgtcggc ctcggtggac tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt
     8581 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     8641 actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac
     8701 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact
     8761 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
     8821 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     8881 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     8941 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     9001 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     9061 tggcaaaccg accagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     9121 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     9181 cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg
     9241 aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca
     9301 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     9361 ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcttccac cgccggatag
     9421 ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc tgccagtaag
     9481 cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca
     9541 tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca
     9601 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcaaccct
     9661 tgtgctgcgc catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta
     9721 atagctgcca gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa
     9781 cgcaatttac gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac
     9841 agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt
     9901 ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
     9961 agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
    10021 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca
    10081 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
    10141 attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
    10201 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
    10261 caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc
    10321 gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc
    10381 ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt
    10441 gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttacgat
    10501 cgcagcggcg gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg
    10561 atggcgctac tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg
    10621 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
    10681 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg
    10741 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg
    10801 attcagggct ttaatgccga cgaaacctct gtaaaagcta ttaccgattt tgccgccgac
    10861 gagctgcacg ttggcgaaat tcatttcctg ccctaccaca cgctaggcat caacaaatat
    10921 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
    10981 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
    11041 ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc
    11101 tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt
    11161 atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc
    11221 tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg
    11281 aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg
    11341 atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc
    11401 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
    11461 ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga
    11521 cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg
    11581 gtctgcgcga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt
    11641 tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc agcgaacaca
    11701 ttgaacgttt tgcagccctg gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc
    11761 gcgatgaact gctggcgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
    11821 ctttctggca ggcgctgcaa ctgtgttact tcatccaatt gatattgcag atcgaatcta
    11881 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg
    11941 acgttgaact caaccagaca ctggatcgcg aacacgccat cgaattgctg catagctgct
    12001 ggctgaaact gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg
    12061 gaagtcctct gtatcagaac gtcaccattg gcggacaaac tctggttgat ggtcaaccaa
    12121 tggacgcggt gaatccactc tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca
    12181 ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct
    12241 gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
    12301 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg
    12361 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
    12421 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca
    12481 aagtgttcct gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag
    12541 taatggacgc gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat
    12601 atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg gcgctggtgg
    12661 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
    12721 ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg
    12781 tatttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
    12841 atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
    12901 acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
    12961 tgaaacagta ccataacccg cgctacggtc gtggtccggt tggcggcaac tattacgcgg
    13021 gtacctcgtc tatctccgct aacgtaccgt ttggcgcgca aactatggca acaccggacg
    13081 ggcgtaaagc ccacaccccg ctggcagaag gcgcaagccc ggcctccggt actgaccatc
    13141 ttggccctac tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg attctcggcg
    13201 gcgtgttgct caaccagaaa ctgaatccgg caacgctgga gaacgaatct gacaagcaga
    13261 aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca
    13321 acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc
    13381 tggtagtgcg tgtcgcgggc tattccgcct tcttcaccgc gctctctcca gacgcacagg
    13441 acgatatcat cgcccgtact gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg
    13501 ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt
    13561 gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct
    13621 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
    13681 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattaagttc
    13741 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
    13801 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc
    13861 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
    13921 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
    13981 gcatttgtcg cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag
    14041 gagattgacg acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta
    14101 gtgatcgaca aactgcacat agcgctcaat ggcattatga aacccgtcac cagtggtttt
    14161 ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
    14221 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
    14281 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
    14341 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
    14401 gcggtgctgg ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc
    14461 cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact
    14521 tatttataac ttatattgtc gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga
    14581 ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat
    14641 ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca
    14701 gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga
    14761 tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca
    14821 gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca
    14881 agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt
    14941 ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta
    15001 ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc
    15061 gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc
    15121 ggatgtgagc gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa
    15181 aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca
    15241 gcgcgttttc gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc
    15301 acatcgtacg gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg
    15361 gacattccaa ggctggacag cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt
    15421 tgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc
    15481 ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca
    15541 tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg
    15601 gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa
    15661 cgtgatgtac attcctgcgg caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt
    15721 gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac
    15781 aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg
    15841 aatcgacagc tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg
    15901 gcgaacccgc cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac
    15961 cggagcatcc gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc
    16021 atcgcgtggc acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc
    16081 gtcgatactc atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt
    16141 taccgttccc gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc
    16201 attggcggca tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct
    16261 gatattcagc aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt
    16321 aatccctgca aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc
    16381 ccggtgaccc atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg
    16441 cgcaatcgcc gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc
    16501 gagatcggcg gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg
    16561 cgtatctgct tcattcatcc gcgagagaaa gagcgcggga tcgctgtcaa actcctccag
    16621 cgcgcagtaa cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac
    16681 acggtaataa aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag
    16741 gttgttcagc aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca
    16801 ggccagcacc tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga
    16861 aaccgtactc ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc
    16921 cattttgtga tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat
    16981 tctgtctggc attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca
    17041 caaaacccgc tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca
    17101 tacaaaaata atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt
    17161 gtaacaactc cattctgacg ttgccgcaca aaccctatac cattctgtgg agtgcatatg
    17221 agtcagggta tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg
    17281 cgttggtgga tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac
    17341 tcgctgggta ttctcgcccc cgaactgaaa agcagcctag ggattaccac cgaacaatat
    17401 tcctggattg taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgcggatgg
    17461 ctgattgacg tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcactg
    17521 gcctgtctga tgcatgcggg ggcgggaaac tgggtacatc tggcgctgtt gcgcttcttt
    17581 atgggcggtg cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt
    17641 ccaaaatcag aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc
    17701 atgctggccc caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt
    17761 atgttcactg gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc
    17821 ccggataagc atcctaacct cagcaaagcc gagctggact ttattcgcca ggataacgaa
    17881 gcgcccccgg tcaagttacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc
    17941 ttttacggta tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc
    18001 tgggtgcctc tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc
    18061 gcctggttgc cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa
    18121 ctttacaccc gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt
    18181 ggcgcattct tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc
    18241 atcgtcctta tctccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca
    18301 ctggtggtgg aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct
    18361 gccgcgtgga tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc
    18421 ggctttaacc ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg
    18481 gtcaccttta ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa
    18541 cgctgaaaaa ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg
    18601 gccagcatat cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac
    18661 gcaaaggcga gctggcgcta gatcgcacct ggagcattgc tccggcgcag gatgtgccat
    18721 gggaagggcg cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acagtgatcc
    18781 agcacgacga caccgtggtt atcgccaccg agtcactgcg tgcaaccgtt caccagccgc
    18841 tgtggctgga gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc
    18901 gcccgaccag cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc
    18961 gtcgtaaaga tgaacgcttt tatggcctcg gagataaagc cggcgatctg caacgcaacg
    19021 gtaaacgtta cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc
    19081 cgctgtacaa acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt
    19141 tttatgacaa cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca
    19201 ccgcctatcg ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg
    19261 aacgcgtact tgatatcacc aaagccttcg tacgcctgac cggaaaaacc ctgttcggac
    19321 cgaaatggag cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac
    19381 aaaatcagtt gatgaacttt atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt
    19441 tccagctctc ttccggttat acctcaatta acggcaaacg ctatgtgttc aactggaact
    19501 acgacaaagt gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc
    19561 tggccgccaa tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag
    19621 aaagaggact gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg
    19681 atgacgaagg ttctaacctc gactttacta atccgcagac catccagtgg tggcaggaag
    19741 gcgtgaccac acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat
    19801 tcgaagtatg ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac
    19861 atattcgccc agtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt
    19921 tcgctccgga aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct
    19981 acgtgcagac ctggagtggc gataaccgca ctagctggga cactctgcgc tacaacaccc
    20041 gcatgggtct ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt
    20101 tctccggtga taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc
    20161 atccgcgctt tacgattcac tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt
    20221 atccgggcgt cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct
    20281 acttttatac cctgatgtgg caggcatacg ccgatgatga accaatgcta cgtccgactt
    20341 tccttgacca cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc
    20401 gcgatatttt ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc
    20461 cagataacga aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt
    20521 ggatcaccct cgacgcaccg ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc
    20581 taccgctcag caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga
    20641 aactgttccc gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg
    20701 aaagctgggg ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg
    20761 gggcgaccat caacctgaaa gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac
    20821 tgaaagtgtc attaccattg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga
    20881 gtgaatggat gcgttaaatc attaccgggg ccgggaggcg actgcacctt acccggtcca
    20941 gccatcgaat cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc
    21001 cgggagctta aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat
    21061 gaaagacggc ggggaattaa gcgtcgatcc ctttactacg caggtaatct tcgtaattac
    21121 cgctaaagtc gatcgcgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta
    21181 cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt
    21241 tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt
    21301 tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca
    21361 ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta
    21421 ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat
    21481 cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc
    21541 attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt
    21601 taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga
    21661 gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt
    21721 tacggaacag tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt
    21781 tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt
    21841 tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct
    21901 tcttggcgtt atcggccagc agacgttcac gcgcctgggt cgccgccgtc atgtactcat
    21961 cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga
    22021 ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca
    22081 gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca
    22141 ggagaatatc cggatcagca aacagcgcct gcgccagaag cacacgcagt ttccagccag
    22201 gagcaacttc actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca
    22261 gttcaccggc gcgagcttcc gcagagtaac cgtccatttc gccgtattta acttccagat
    22321 cggccacttt atagccgtct tcctcactca tttccggcaa agcatagatg cggtcacgct
    22381 cctgcttcac ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact
    22441 cttcaaaggc aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa
    22501 cgttacccaa agtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac
    22561 taccgttcgc gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt
    22621 caaacaacgg cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat
    22681 cctgaaaaga gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc
    22741 ctcgcttaag gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt
    22801 tcactgtgag ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa
    22861 tggcctgcca ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca
    22921 gcggccttcg ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca
    22981 accgacggga gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact
    23041 cagccgctgg agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg
    23101 aacccgggtg tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag
    23161 ctgatcctgc cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg
    23221 tattactatc cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta
    23281 ggcaaagata cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc
    23341 tggacgccga ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct
    23401 gtcgttccgg caggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg
    23461 tacgctatcc atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt
    23521 gtgcgtctgc gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc
    23581 gtacagttta ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt
    23641 gaagtccata acccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca
    23701 attaccctga cgaagagcgt gcaaacagtg accggtcagc cagatgttga ccaggttgtt
    23761 cttgatgaag cgattaaaaa ccgctccggg atgccggttc gtctgaatta atcttcaaaa
    23821 cttaaagcaa aaggcggact cataatccgc cttttttatt tgccagaccc tagttggccg
    23881 ggagtataac taacaaaaca tatcccacca acgccgccca caacagcatc ggtattgaat
    23941 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    24001 ccagcgatcc gggtaataac ccaaagccac ctacgtttac cgcccatacc agtaataaag
    24061 acggcggcac atagttcagc aacaatatgg tactcggcac gttactgatc acctgcgata
    24121 aaccgattgc cgttaaccat aacccgggtt cagatagatg actcacgtta cccaacacgc
    24181 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    24241 gcagcagcgt ccagtccaca ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg
    24301 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat
    24361 acagccccag acaactccac accagccgcg gtttccactc cggtgtttgc acccccgtat
    24421 ggtattgcag cgcctttcca gggaaacaac accagcacag gagcaggagc gtcagcatca
    24481 ttgcgccagc cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    24541 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    24601 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    24661 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    24721 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    24781 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    24841 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    24901 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    24961 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    25021 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    25081 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    25141 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    25201 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    25261 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    25321 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    25381 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc
    25441 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    25501 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctacat
    25561 aatatagcct gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    25621 gcaagtcagg aggtcttatg aatgagttca agaagtgtat gcgcgtgttt agtcattctc
    25681 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    25741 agcaagataa accttccgat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    25801 atgcaggcat aacgcgtttt ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc
    25861 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    25921 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    25981 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg
    26041 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    26101 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    26161 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    26221 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    26281 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    26341 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    26401 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    26461 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    26521 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    26581 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    26641 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggtg
    26701 gtgatcggcg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    26761 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc
    26821 tccacgcaga aatcacttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    26881 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    26941 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    27001 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    27061 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    27121 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    27181 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    27241 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    27301 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    27361 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcgacga taaagcgcat
    27421 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    27481 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    27541 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    27601 attttcgacc cgaaacctta aaacaaaaaa tccgccccga gaggcggatt ttttatatca
    27661 ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt caacgctacg
    27721 aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt tgaactgcag
    27781 accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt
    27841 ggtctggaat ttaccataac ccacccccac tacaccgtag atgcttgccc agtcgttaat
    27901 gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt caccagagct
    27961 tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc
    28021 ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc cctgagcgtc
    28081 gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac ctgcggtgaa
    28141 agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca cctcaaatgt
    28201 gattcaaata agttctaagt tttaaatata tcaaaaatta atgggaaact cttcgcgatt
    28261 tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc tatccatcta
    28321 aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc ctgtcattca
    28381 tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca ttttagtcaa
    28441 aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc agtacactcc
    28501 acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat tacgtaaaat
    28561 gccggtctgg ttaccaatag tcatattgct cgttgccatg gcgtctattc agggtggagc
    28621 ctcgttagct aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct
    28681 ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa
    28741 agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga attatctttt
    28801 ttatctttct attcagacag taccgctggg tattgcggtg gcgctggagt tcaccggacc
    28861 actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc
    28921 ggttcttggt ctgtggttcc tgctaccgct ggggcaagac gtttcccatg tcgatttaac
    28981 cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt taagtgggca
    29041 acgcgcagga gcggaacatg gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt
    29101 aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact ggtcggttat
    29161 tccattgggt ctggctgtcg ctattctctc gaccgctctg ccttattcgc tggaaatgat
    29221 tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg aaccggcgct
    29281 ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac agctgctggc
    29341 gctcggcgct atcatcgccg cttcaatggg gtctacgctg acagtacgca aagagagcaa
    29401 aataaaagaa ttagacatta attaaattta catttctgca tggttatgca taaccatgca
    29461 gaatttctcg ctacttttcc tctacaccgt ctttatatat cgaattatg
//
LOCUS       DAZPLM010000031        29218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPLM010000031 DAZPLM010000000
VERSION     DAZPLM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.031"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..924
                     /locus_tag="ACHVC7_001156"
     CDS             <1..924
                     /locus_tag="ACHVC7_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2115264.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHVC7_001157"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHVC7_001157"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR2115265.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHVC7_001158"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHVC7_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR2115266.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="ACHVC7_001159"
     CDS             3885..4280
                     /locus_tag="ACHVC7_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR2115267.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="ACHVC7_001160"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="ACHVC7_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR2115268.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="dolP"
                     /locus_tag="ACHVC7_001161"
     CDS             4900..5475
                     /gene="dolP"
                     /locus_tag="ACHVC7_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR2115269.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="ACHVC7_001162"
     CDS             complement(5589..6629)
                     /locus_tag="ACHVC7_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2115270.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="ACHVC7_001163"
     CDS             complement(6702..7337)
                     /locus_tag="ACHVC7_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR2115271.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHVC7_001164"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHVC7_001164"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR2115272.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="ACHVC7_001165"
     CDS             complement(7963..8406)
                     /locus_tag="ACHVC7_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR2115273.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHVC7_001166"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHVC7_001166"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR2115274.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="ACHVC7_001167"
     CDS             complement(8746..9249)
                     /locus_tag="ACHVC7_001167"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2115275.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="ACHVC7_001168"
     CDS             complement(9243..9767)
                     /locus_tag="ACHVC7_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR2115276.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="ACHVC7_001169"
     CDS             9976..10971
                     /locus_tag="ACHVC7_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR2115277.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="ACHVC7_001170"
     CDS             10980..11858
                     /locus_tag="ACHVC7_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR2115278.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="ACHVC7_001171"
     CDS             12064..13071
                     /locus_tag="ACHVC7_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR2115279.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHVC7_001172"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHVC7_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR2115280.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHVC7_001173"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHVC7_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR2115281.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHVC7_001174"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHVC7_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR2115282.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHVC7_001175"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHVC7_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR2115283.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHVC7_001176"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHVC7_001176"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR2115284.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHVC7_001177"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHVC7_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR2115285.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHVC7_001178"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHVC7_001178"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR2115286.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHVC7_001179"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHVC7_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR2115287.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHVC7_001180"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHVC7_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR2115288.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHVC7_001181"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHVC7_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR2115289.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHVC7_001182"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHVC7_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR2115290.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="ACHVC7_001183"
     tRNA            complement(26829..26905)
                     /locus_tag="ACHVC7_001183"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
     gene            27211..28383
                     /gene="istA"
                     /locus_tag="ACHVC7_001184"
     CDS             27211..28383
                     /gene="istA"
                     /locus_tag="ACHVC7_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR2115291.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            28383..29180
                     /gene="istB"
                     /locus_tag="ACHVC7_001185"
     CDS             28383..29180
                     /gene="istB"
                     /locus_tag="ACHVC7_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR2115292.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT         6982 a         8027 c         7406 g         6803 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcag tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga taagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct caatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaagctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaagt gtcagcgcca
    27121 gtgatataag acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct
    27181 taaggttctc acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata
    27241 aagcaaatgc gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct
    27301 gaacggacgg tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac
    27361 aaaatggtta agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc
    27421 tggaatagcg aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc
    27481 atgctgcgtt actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc
    27541 ttcgaaactc agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt
    27601 gccgggcaac ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc
    27661 catgtcttcg ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc
    27721 ttccgctact tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg
    27781 ctgaagaata acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac
    27841 tataacttcc tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag
    27901 cggatggtga aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc
    27961 actcatgtca atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt
    28021 cgccagttca aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg
    28081 ttaccggata cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc
    28141 tatatcgagg ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta
    28201 tcgatacgaa tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc
    28261 tcacatcgcc tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg
    28321 ctctggcagc aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg
    28381 tgatgcatga actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg
    28441 ttgaaagcct gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca
    28501 tggcgctaca gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc
    28561 aggctcgtct gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca
    28621 tcgaccgtaa ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg
    28681 tgatcctgct gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga
    28741 aagcggtgga tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac
    28801 tgatgaaagc gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc
    28861 gggtgttgat cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt
    28921 tcttccggct actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag
    28981 ggttcgcaga ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc
    29041 ggctgctaca tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga
    29101 aacgtaaagc tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg
    29161 aaagcggaca gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZPLM010000032        28050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPLM010000032 DAZPLM010000000
VERSION     DAZPLM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.032"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHVC7_001186"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHVC7_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR2115293.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHVC7_001187"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHVC7_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHVC7_001188"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHVC7_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHVC7_001189"
     CDS             complement(1702..3318)
                     /locus_tag="ACHVC7_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR2115294.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHVC7_001190"
     CDS             3614..4330
                     /locus_tag="ACHVC7_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR2115295.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHVC7_001191"
     CDS             complement(4327..5988)
                     /locus_tag="ACHVC7_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR2115296.1"
                     /translation="MNDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHVC7_001192"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHVC7_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR2115297.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHVC7_001193"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHVC7_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR2115298.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHVC7_001194"
     CDS             7443..7775
                     /locus_tag="ACHVC7_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR2115299.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHVC7_001195"
     CDS             complement(7777..8991)
                     /locus_tag="ACHVC7_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR2115300.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHVC7_001196"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHVC7_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR2115301.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHVC7_001197"
     CDS             complement(10153..11490)
                     /locus_tag="ACHVC7_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115302.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHVC7_001198"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHVC7_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHVC7_001199"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHVC7_001199"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR2115303.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHVC7_001200"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHVC7_001200"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR2115304.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHVC7_001201"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHVC7_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR2115305.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHVC7_001202"
     CDS             15153..15809
                     /locus_tag="ACHVC7_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115306.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHVC7_001203"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHVC7_001203"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR2115307.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHVC7_001204"
     CDS             complement(16782..17180)
                     /locus_tag="ACHVC7_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR2115308.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHVC7_001205"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHVC7_001205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR2115309.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHVC7_001206"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHVC7_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR2115310.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHVC7_001207"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHVC7_001207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR2115311.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHVC7_001208"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHVC7_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR2115312.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHVC7_001209"
     CDS             23741..23821
                     /locus_tag="ACHVC7_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115313.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHVC7_001210"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHVC7_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR2115314.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHVC7_001211"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHVC7_001211"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR2115315.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHVC7_001212"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHVC7_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR2115316.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHVC7_001213"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHVC7_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR2115317.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHVC7_001214"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHVC7_001214"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR2115318.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7569 c         7159 g         6562 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcattcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca actgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZPLM010000033        27789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPLM010000033 DAZPLM010000000
VERSION     DAZPLM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.033"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            43..705
                     /gene="acrS"
                     /locus_tag="ACHVC7_001215"
     CDS             43..705
                     /gene="acrS"
                     /locus_tag="ACHVC7_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR2115319.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(708..887)
                     /locus_tag="ACHVC7_001216"
     CDS             complement(708..887)
                     /locus_tag="ACHVC7_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR2115320.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(971..1855)
                     /gene="yhdJ"
                     /locus_tag="ACHVC7_001217"
     CDS             complement(971..1855)
                     /gene="yhdJ"
                     /locus_tag="ACHVC7_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR2115321.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1941..2237)
                     /gene="fis"
                     /locus_tag="ACHVC7_001218"
     CDS             complement(1941..2237)
                     /gene="fis"
                     /locus_tag="ACHVC7_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR2115322.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2263..3228)
                     /gene="dusB"
                     /locus_tag="ACHVC7_001219"
     CDS             complement(2263..3228)
                     /gene="dusB"
                     /locus_tag="ACHVC7_001219"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR2115323.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3557..4438)
                     /gene="prmA"
                     /locus_tag="ACHVC7_001220"
     CDS             complement(3557..4438)
                     /gene="prmA"
                     /locus_tag="ACHVC7_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR2115324.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4450..5901)
                     /gene="panF"
                     /locus_tag="ACHVC7_001221"
     CDS             complement(4450..5901)
                     /gene="panF"
                     /locus_tag="ACHVC7_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR2115325.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5891..6133)
                     /locus_tag="ACHVC7_001222"
     CDS             complement(5891..6133)
                     /locus_tag="ACHVC7_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR2115326.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6242..7591)
                     /gene="accC"
                     /locus_tag="ACHVC7_001223"
     CDS             complement(6242..7591)
                     /gene="accC"
                     /locus_tag="ACHVC7_001223"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR2115327.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7602..8072)
                     /gene="accB"
                     /locus_tag="ACHVC7_001224"
     CDS             complement(7602..8072)
                     /gene="accB"
                     /locus_tag="ACHVC7_001224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR2115328.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9050..10024)
                     /locus_tag="ACHVC7_001225"
     CDS             complement(9050..10024)
                     /locus_tag="ACHVC7_001225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR2115329.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10176..12116
                     /gene="csrD"
                     /locus_tag="ACHVC7_001226"
     CDS             10176..12116
                     /gene="csrD"
                     /locus_tag="ACHVC7_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR2115330.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12421..13464
                     /gene="mreB"
                     /locus_tag="ACHVC7_001227"
     CDS             12421..13464
                     /gene="mreB"
                     /locus_tag="ACHVC7_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR2115331.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13530..14633
                     /gene="mreC"
                     /locus_tag="ACHVC7_001228"
     CDS             13530..14633
                     /gene="mreC"
                     /locus_tag="ACHVC7_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR2115332.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14633..15121
                     /gene="mreD"
                     /locus_tag="ACHVC7_001229"
     CDS             14633..15121
                     /gene="mreD"
                     /locus_tag="ACHVC7_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR2115333.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15130..15723
                     /gene="yhdE"
                     /locus_tag="ACHVC7_001230"
     CDS             15130..15723
                     /gene="yhdE"
                     /locus_tag="ACHVC7_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR2115334.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15713..17182
                     /gene="rng"
                     /locus_tag="ACHVC7_001231"
     CDS             15713..17182
                     /gene="rng"
                     /locus_tag="ACHVC7_001231"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR2115335.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17250..21050
                     /gene="yhdP"
                     /locus_tag="ACHVC7_001232"
     CDS             17250..21050
                     /gene="yhdP"
                     /locus_tag="ACHVC7_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR2115336.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21206..22651
                     /gene="tldD"
                     /locus_tag="ACHVC7_001233"
     CDS             21206..22651
                     /gene="tldD"
                     /locus_tag="ACHVC7_001233"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR2115337.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22779..23708)
                     /gene="aaeR"
                     /locus_tag="ACHVC7_001234"
     CDS             complement(22779..23708)
                     /gene="aaeR"
                     /locus_tag="ACHVC7_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR2115338.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23891..24094
                     /gene="aaeX"
                     /locus_tag="ACHVC7_001235"
     CDS             23891..24094
                     /gene="aaeX"
                     /locus_tag="ACHVC7_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR2115339.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24102..25034
                     /gene="aaeA"
                     /locus_tag="ACHVC7_001236"
     CDS             24102..25034
                     /gene="aaeA"
                     /locus_tag="ACHVC7_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR2115340.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25040..27007
                     /gene="aaeB"
                     /locus_tag="ACHVC7_001237"
     CDS             25040..27007
                     /gene="aaeB"
                     /locus_tag="ACHVC7_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR2115341.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27099..27371
                     /locus_tag="ACHVC7_001238"
     CDS             27099..27371
                     /locus_tag="ACHVC7_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR2115342.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27427..27690)
                     /gene="yhcN"
                     /locus_tag="ACHVC7_001239"
     CDS             complement(27427..27690)
                     /gene="yhcN"
                     /locus_tag="ACHVC7_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR2115343.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6673 a         6964 c         7382 g         6770 t
ORIGIN      
        1 ctcaccctgt gtcgaatata tttatttcct gaataattaa tcatggcaaa aagaaccaaa
       61 gccgaagctc tgaagacccg gcaagaactg attgaaactg ccatcgccca gtttgcgcaa
      121 catggcgtaa gcaagacgac gttcaacgac attgccgatg ccgctaacgt tacgcgtggc
      181 gctatctact ggcacttcga gaacaagacc caactgttta atgagatgtg gttgcaacag
      241 ccttcattgc gggagttaat ccaggaacac ttgacggctg gattagagca tgacccgttt
      301 caacaattgc gtgaaaaatt gattgtcggc ttgcaatata ttgccaaaat tccccgccag
      361 caggcgttgc tgaaaatctt atatcacaaa tgtgaattta atgatgggat gctggccgag
      421 ggagtgatac gcgaaaagat gggctttaat ccgcagactc tccgcgaagt attgcaggcg
      481 tgtcagcaac aaggttgtgt agcaaataac ctcgatttag atgttgtgat gattattatt
      541 gatggtgcct tcagcggaat tgttcaaaac tggttaatga atatggcggg ttatgatctt
      601 tataaacaag cacccgcgct ggtcgataac gtattaagaa tgttcatgcc agatgaaaac
      661 ataacgaaat taattcatca aacgaatgaa ttaagtgtca tgtaaattca cattctgtcc
      721 tgaaaattca gttcgtcaca gttaaccagt tttagtggat aaactgagtt catattacgg
      781 catttgtctg tttctgttgt cagcagttca atccactgca tcagtaaagt atcaaacaga
      841 aaaacgaaga cagcgaaaac gacgagccac caatacttgc gaatcattac ctgagcctgt
      901 gcatgtttaa ccaagtctgg caggaaatat acacgaaatg aataatctga aaaccaggct
      961 tacacttcct ttactttgca atgagatcgg ggtcaacttc gcttaaccgg cttcgttttg
     1021 acaggttgcc ggtctttctt tttttcactt tcgccagttc ttccgcggag taatgcgacg
     1081 cgacatccag ccgtcgaagc cccattttga tgtactcgct gttgatctca ataccaatga
     1141 attttcgtcc gctggcgatg gctacggcac cggtagtaaa gctaccggca aacgggtcga
     1201 gaacgatatc gcctgggttg gaagaggcga gaataatgcg tttcagtaag gcttccggtt
     1261 tttgcgtcgg gtggttctca tattcatcca tcaaataacg cacgcgcgga aaatcccaga
     1321 cgttacccgg tactttttga tgattgtatg gctgtggagg atttttgcga taatcgatca
     1381 acgcgcgctg cgatccggtt ttggcttcta ccagaatagc atcaccgttg aatgtgtagt
     1441 tctttgcgtc tttcaccatc atcaggatgg gttcgtacat ggagccgtag tgttttttcg
     1501 cctgtactcc agaactgtca tatgaccaga cgatgcgact tttgatggta aaaagcttgc
     1561 ggcactggag atcgataaag ggcatgtttt ccgtactgtt cataatgtac atgctgccct
     1621 gctttttcag aacgcggtgg cactctacaa tcacttcaaa cagccagtcg ataaacagat
     1681 cttctttcca ggcttcgatc agaccatcaa aatttttacc gatgttatac ggtgggtcgg
     1741 caaagatcag atcgacgctt tcagtaggta gctttttaag ttcggcaagg gcatcaccgt
     1801 gaataatggt cttagcttca ttaccaaacc gggtcggttc acatcctgtt ctcatggtca
     1861 ctccctttgt gacacctata aaaaaggcgc ttccccatgc cgagtagcgc ctttttaatc
     1921 aagcatttag ctaacctgaa ttagttcatg ccgtattttt tcaatttttt acgcagcgta
     1981 ccacggttga tgcccatcat cagcgcagca cgggtctggt taccacgggt gtattgcatc
     2041 accatgtcca acaggggctg ttctacttca gccagtacca gctcatagag gtcattcaca
     2101 tcctgaccat tcagttgagc aaaatagttc ttcagtgcct gtttaaccga gtcacgcagg
     2161 ggtttttggg ttacctgatc ctgagagtta acggtagaaa cggtcagtac gtcagaattt
     2221 acgcgttgtt cgaacatagt tctgtcagct ctttatttct gtttacgcaa aattttcgaa
     2281 gtatgcctcc aacgcctcca gctgttcgct ggcatcctca atggcgttga atgtgcgccg
     2341 aaactggtca tttggagcgt gttcctggag ataccaggaa acgtgtttac gtgcaattcg
     2401 gtaccctttt gccggaccat aaaagtcatg cagttcccga acgtgcgcgc aaagcaagcg
     2461 cttaacctct gccaaaggca gcgggggcag caactcccca gtgtccagat aatgctggat
     2521 ttcccgaaag atccagggtc ttccctgagc tgcgcggcct atcatcaggg catccgcccc
     2581 tgtatagtcg agcacagctc tggctttaag cgggtcagta atgtcgccat tcgcgataac
     2641 cggaatggaa actttctgct taactgcccg aatactgtcg tactcagctt ctccattgaa
     2701 caaacaggcg cgtgtacggc catgaatggt cagagcctga atgccacagt cttcagccag
     2761 ttgggcaatc tcttcgcagt tacggtgttc cggtgcccag ccggtgcgaa tcttcagggt
     2821 aacaggaacg tccactgcat tgacgacctc ggtaaggatc gatttaacga catccgggta
     2881 ctgcaagagg gctgaacctg cgagcttgcg attcactttt ttagccgggc aacccatatt
     2941 gatatcaata atctgggcac cgctttccac gttaatacgt gctgcatctg ccatttcttt
     3001 cggatcgcta ccagcaattt gcacggtgcg aataccgggt tcatcaatgt gcaccatccg
     3061 taaacgagat ttgtcgcttt cccaaacctg tgggttagaa gacatcatct cggatactgt
     3121 caatccggct cccatctcgt agcacaacgt ccgaaaaggt ctgtctgtaa tgccagccat
     3181 gggcgctgcg atcaggcgat ttctgagctg atattgtccg atgcgcatga gttaagaaat
     3241 gaccatactg tgactgcaag gcggcgtata ttacgcattt tttgcacgag atgaaaggcc
     3301 aaactttgac caatcctctg agatggatca aagaattgca tttaaaatga gcgtggtgag
     3361 ataattactc ataaaaatca tcatattaga aaatagtgac taaaatttac actcaaagaa
     3421 atttgagtaa gttctcaatt tttctttatg aatgaaaatt ttggcacgca aatttgcgta
     3481 aataaccggc aaatttacgt gccttttgtg agcttgctcg cacttcgccc cgcgtcaccc
     3541 tacggcgatg cgaaggttaa ttcttacgac cggtaatgcg gcaccactct tctttttcca
     3601 cgaccgggtc cagtgcgaag ctgtcgacat aagcttcaca aacgctctct gcctggcttg
     3661 ccagaatacc ggaaaggccc agcaaaccgc ctgaaaccgg caggacgctg attaacggtg
     3721 ccagttcacg taatgggcct gcaaggatgt tagcgaccac cacgtcggct ttcatttctt
     3781 ctggctgatc tttcggtaag tagagttcca gacggtcaga aacgccatta cgttcggcgt
     3841 tgtcgcggct agcctgaatc gcctgcggat cgatatcaat accaatggct tttgctgcac
     3901 ccagtttcag cgccgcgata gccagaatgc cggaaccaca gccaaagtcg atgactattt
     3961 taccggttaa atcgaggctg tcgagccact gcaggcacag agaggtagtt ggatgggtac
     4021 ccgtaccaaa cgccagccct ggatctaaca tcacgttgac ggcgttttcg tccggcacat
     4081 cacgccagct agggcagatc cacagtcgtt caccaaagcg catcgggtgg aaattatcca
     4141 tccattcgcg ctcccagtct ttatcttcca gttgttcgat tttatgcgcg aagcctgcgc
     4201 cgagcagcgg atggttttcc agaatcgcca ccacgtcgtt catatcggtt tcagcgtcga
     4261 acagaccaat cacatcggtg tcgccccaca ggcgcgtttc gcccggcagc ggctcaaata
     4321 ctggcgtatc gtgggtatcc tgaaaagtga tagaaacggc acccgcttcc atcagcgcat
     4381 cgctaagatc ttcagcgttc gcgccggtgg tgttcagttt cagttggatc caaggcatgg
     4441 caaaactctt tatttatcag tagtcaaaac ggtagcttgc gggacggatg taccgaaacg
     4501 gtttccgacc aggaaagcca gcaaacttag tagtaacgag ggcacgatag ggtggaagcc
     4561 caggtactga atattcagcg tcgcgagtac ggcatacagc acgccgccaa cgatcatcgc
     4621 acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga cccagcacca gcggccacag
     4681 gaaaacggct tccagcccac cgaaggccag caaattcagc cagatgatca tttctggcgg
     4741 cttccaggcg gcaagcagca gcaacgcgcc gagaactaac gtaattaccg ccgacatccg
     4801 cttcagacgc gtctcgtttt gcatttgatc cggacggata ttcagataga gatctttaat
     4861 gatcgtagcg gaactttgta gcaattgggc gttaattgtc gacatgatcg cagccatagg
     4921 tgcagccaga aagatcccgg cagcaaacgg tggcagcact tttaccatta acgtcgggat
     4981 caccagatcc ggtacggtga gatcggggat cactgcacga cctaacgctc cggctaggtg
     5041 cataccgaac atcaggattg cgactacaat cgtaccgatg atgatccccc ggtgtaccgc
     5101 tttgctgtct ttataagaga tacagcgcac cgcagtatgc ggcaggccaa tcacgccaaa
     5161 gcacaccagt acccagaacg acgtcataaa ggcaggcgac agaatatcgt cagcgccttg
     5221 tggcgtaacc agttgcggat cgatggtttg caaggtctgt actgcgttgc ttaagccgcc
     5281 agcggcatgt accacgccaa taagcagcac aacggtgcca atcagcatca caagcccttg
     5341 catggtgtcg ttcagcacgc tggcgcgaaa gccgccaaag gcggtataca acgcaatgct
     5401 gataccgaaa atcagcagcc cggtttcata aggaataccc gccgcggttt ccagcaggcg
     5461 cgcaccgccg ataaactgca cggtcattgc cccaacgaac gcaaccagca aactcaaact
     5521 cgccagccac accagaagac gactctggta gcgggcaaac agcatgtcgt tcagcgtcac
     5581 tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg agaataccga gtgaaagcca
     5641 gactgcagga agctgaatca tcgccagcaa tacccagccc agcccgtatt tataagcagc
     5701 tcctggcccg ccgataaacg aactggcact gatataggtc gcggtgagcg tcatcgccag
     5761 cacaataccg cccatagagc ggctgccgag gaaatactca ttaaggaagg tgccggtgct
     5821 ccgtttacgc atcgcataaa ccgagatacc gaacaccacc accagatagg cgaccagcgg
     5881 tagaattact tcaagctgca tcgtcatcct ccagtgggat atcgcgatag ataaatttca
     5941 ccatcgccca gcacagtcca ataaacagta gcggcgtcag gatgcaggcc atctcaaacc
     6001 agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa gtaagcggct actaaccaaa
     6061 ctgccagata caaaagggtc agccccagcg cccagcgcgc ctctttatgg gcctgaacaa
     6121 aacgagtgtc cattttttgt ccctgatggg tgaagaaagc gaggattgta ccttatgggg
     6181 gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg ccttttgacg cttttgcagt
     6241 cttatttttc ctgaagaccg agttttttct ccagatagtg gatgttagtg ccaccatgct
     6301 ggaagttctc gtcattcatg atgcggatct gcagatcaac gttggttttg ataccgtcga
     6361 tgatcagctc ctgcagcgca ttcttcatgc gggcaatcgc cacgtcacgg ttttcaccgt
     6421 agcaaatcag cttaccgatc attgagtcat agtacggcgg tacggtgtag cccgcgtaga
     6481 tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg gaaacgggtg attttgcctg
     6541 ggcttggcag gaaggtgttc ggatcttcgg cgttgatacg acattccacc gcatggccgc
     6601 gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc ggcagcgata cggagctgtt
     6661 ctttgatcag gtcaacgcca gtgatcattt cggtaaccgg gtgttctacc tgaatacggg
     6721 tgttcatttc gatgaaatag aactcgccgt tttcgaacag aaactcgaaa gtacctgcac
     6781 cgcgatagcc gatatcaaca cacgctttag cgcaacgttc gccgatgtag cgacgcagtt
     6841 ccggggtaat gcccggtgct ggcgcttctt cgaccacttt ctggtggcgg cgctgcatgg
     6901 agcagtcacg ttccgccaga tagatagcgt tgccctgacc gtcagccagt acctgaatct
     6961 cgacgtggcg aggattttcc aggtatttct ccatgtaaac catatcgttg ctgaaagcag
     7021 ctttcgcttc cgcacgggtc atggagatgg attgtgccag ttcagcgtcg ccgcgcacta
     7081 cgcgcatacc gcgaccaccg ccgccgccgg aggctttgat aatcaccgga taaccaatgc
     7141 gtttagcaat ggcacggttt ttatccatat cgtcgcccaa cgggccgtca gaacccggta
     7201 cgcaagggac gcccgctttt ttcatcgccg cgattgcgga tactttgtcg cccatcaggc
     7261 gaatggtttc tgctttcggg ccgatgaaga taaagccgga gcgttcaacc tgctcggcaa
     7321 agttggcgtt ctcggagagg aagccgtaac ccggatggat tgctactgcg ccggtgattt
     7381 cagcggcgct gatgattgcc gggatgttca gataactttt tactgacgga gcagggccaa
     7441 tacagaccgt ttcatctgcc agtaatacgt gttttagatc gcgatccgcg ctggagtgca
     7501 cagcgacagt cttgatgccc agttctttac aggcacgaag aatacgcaat gcaatctcgc
     7561 cgcggttggc aataacaatt ttatccagca tgttcgcctc gttactcgat gacgaccagc
     7621 ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa ttgctttcac ggtaccggat
     7681 ttgtccgctt cgatctggtt catcattttc atggcttcaa cgatgcacag ggtatcgccc
     7741 acgttgactt tctgacccac ttcgatgaac gcttttgcgt ccgggcttgg ggtgcggtag
     7801 aaagtaccaa ccatcgggga acgtacgatg tgaccactga tttccgctgc tgctggcgct
     7861 tccatggaag gaacggtcgc cggagcggct gcgttagatt gagctggctg ctgcatcatt
     7921 ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg caggagctgc acggctaatg
     7981 cgtactgact cttcgccttc agaaatttcc agttcggaga tgcctgattc ttcaaccagc
     8041 tcgatcagtt ttttaatctt acgaatatcc atgagtgggt tccgtactct ttgtttagtg
     8101 tgattgtgac aggcgtttca ccgccgtctg taaagcgtat gccccgacag ctgtatgcat
     8161 agcgataaat tccagcaggc cgggacgccg gtctattttg ccccagagta tcaatgttag
     8221 acttgacgga cattgtgcag cgatctgctg ctatcctccg gcaaaaaaca aaatatacct
     8281 tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg cgtcagggag gacgaggtcg
     8341 cacattataa cgatttcgta gcaattggca gctaaatact ggtctcatca gggaagataa
     8401 tcaacagcta acatgtaaat aaccttcaac accgtgtaat ttgcaacaag ccgcacaatt
     8461 cacgaaatta gcgccatcat cgacggaact tcttataacg taaggtgaaa agctgtaaag
     8521 ccagccctgc gaagatgagg ggctgcaatg agataatctt cacagaccac aaataatgta
     8581 gggatgccag gatcgcgaca agatagacga cattacgcag cggttgctgg ggattaccca
     8641 attttcactg catcgtctgg atagatgtga atgctaaagc acgcaaaatc aaccgactga
     8701 caattctcag cattaaatag gttcgagtgg ttaatttgta gtcaaataaa atcagattat
     8761 tcaccccatg gtaccagaaa tcagtaacag gttaagcgta gtgtcgccca ggcaaagcac
     8821 cataaaccta acaggcgacg gaggcgtatc aataatggct gtttagagga gtatcaatgc
     8881 gcatcaattt gtggtcagtc gaaatcatga ctttccagtt tggatgatgt tgtacaactt
     8941 cctgaccaag gtgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga
     9001 ataatgatgt caaaatgagt tccctctctt attattcctt gctaaatggt tagttaacct
     9061 tcaccagcgt gcgaccctgg atctggttat taatgatggc ctcggcaaag ttcggtgcct
     9121 ctgacagaga tatctctttt gccgcctggg tatagaatga ttccggtaaa tcggcgacca
     9181 gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat tactgaatcc accccttgca
     9241 aacggacatt acgcagaata aatggcatga ccgtggttgg cagagtaaaa ccacccgcca
     9301 gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc cagcactttt gccagcactt
     9361 tgtcgccaac agtgtcaatt gccccagccc agacttgttt ttccagagga cgggattcgg
     9421 caaactcatc acgagggaga atacggctgg cacctaaact tttcagatat tcatgggtac
     9481 tttcgcgacc ggaaacggca acgacctgat aacccaactt atgcagcagc gccacggcgg
     9541 tactgccgac gccaccactg gcacccgtca cgacaatctc cccgtcctgc gggcgaacac
     9601 cggcatcttc cagcgccatc acacacagca tggcggtaaa accggcagta ccgataatca
     9661 ttgctttacg cgcgtccagc ccttgcggca tggcaaccag ccagtcacct ttcactcgcg
     9721 cctgctccgc cagcccaccc cagtggtttt caccaacgcc ccagccagtg agtaacacct
     9781 cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac agttccggca aaatcgatcc
     9841 caggaatcat cggaaaatta cggatgattt ttcccttacc ggtaatcgcc agcgcatctt
     9901 tatagttcag gctcgaccag tgaacatcga ccgtgacatc gccctccggc aggcgacttt
     9961 cgtccagagt ctgtactgat gcgagagttt tgccatcctg ctgttctaaa agtaacgcct
    10021 gcataagtgg tcctcatgtg catgatggat tggaaaataa tttctgaaga ttatactcgc
    10081 taatggaaaa gcaaagccga tgaagcgcaa taaattgcga gataaatctg atttgctagt
    10141 atgcccgctt cctcactatc ggagttaaca caaggatgag attaacgacg aaattttcgg
    10201 cctttgttac gctgctcacc gggttaacaa tttttgtgac tttgctgggc tgttcgctaa
    10261 gtttctacaa cgcaattcag tataagttta gtcatcgcgt tcaggcggtg gcgacggcga
    10321 ttgataccca ccttgtgtcg aatgacttca gcacattaag gccacaaatt accgaattaa
    10381 tgatgtcggc agatatcgtt cgtgtagacc tgctccatgg tgataaacag gtttataccc
    10441 tggccagaaa tggtagttat cgtccggttg gctccagcga tctgtttcgc gaactgagtg
    10501 ttccgttgat aaagcatccg gggatgtcgc tgcgtctggt ttatcaggat ccgatgggca
    10561 actatttcca ttctttaatg actaccgcgc cgctcacggg ggcgattggc tttatcattg
    10621 ttatgctctt cctggcggta cgctggttac aacggcaact tgccgggcaa gaattgctgg
    10681 aaacccgggc tactcgtatc ttaaacggtg agcgtggctc taatgtgttg ggaaccatct
    10741 atgaatggcc gcccagaacc agcagtgcgc tagatacgct gcttcgtgaa attcagaacg
    10801 cacgcgaaca acacagccgt cttgatacgc tgatccgctc ttatgccgcc caggacgtga
    10861 aaaccggcct caataaccga ctctttttcg ataatcagtt agcaacgtta ctggaagatc
    10921 aggagaaagt aggtacccac gggatcgtga tgatgattcg tctgccggat ttcaatatgt
    10981 tgagcgatac ttgggggcac agccaggttg aagaacagtt cttcactctg actaatctgc
    11041 tgtcgacatt tatgatgcgc taccctggcg cactgctggc gcgttaccac cgcagtgatt
    11101 ttgctgcgct gttaccgcac cggacgttaa aagaggcaga gagcatcgcc ggtcagttaa
    11161 tcaaagccgt cgataccttg ccgaacaata aaatgctcga tcgcgacgat atgatccaca
    11221 ttggtatctg cgcctggcgt agtggtcagg ataccgagca ggtaatggaa catgcagagt
    11281 ctgccacgcg taatgcggga ttgcagggcg gcaatagctg ggctatttac gatgactcgt
    11341 tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc
    11401 gcggcggccc gcgcctttat caaaaaccgg cggttactcg cgaaggtcag gttcatcatc
    11461 gcgaactcat gtgccgcatc ttcgatggta atgaagaggt tagctcggcg gagtatatgc
    11521 cgatggtctt gcagtttggc ttatcggaag agtatgaccg tctgcaaatc agccgtctta
    11581 ttccactatt gcgttactgg ccagaggaaa atctggcgat tcaggttacc gttgagtcgc
    11641 tgattcgccc gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcac
    11701 aacgaaaacg cataattatt gaacttgcag aggccgatgt aggtcaacat atcagtcgtt
    11761 tacaacctgt tattcgttta gtgaatgctt taggggtacg ggtagccgtc aaccaggctg
    11821 gtttgacgct ggtaagcacc agttggatca aagaacttaa tgttgagtta ctcaagctcc
    11881 atccggggct ggtcagaaac attgagaagc gaacggagaa ccagctgctg gttcaaagcc
    11941 tggtggaagc ctgctccggg accagcaccc acgtttacgc caccggcgtg cgttcgcgaa
    12001 gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg gcaaggggat tttttcgcgt
    12061 cctcacagcc acttgatact aacgtgaaaa aatattcaca aagatactcg gtttaacctg
    12121 ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc
    12181 tgctcgccag attgttgcag cacatatgca gatgaatgac cttacgcggt tgcaaacagg
    12241 cgaggaatgc tgctgatgca ttaagccttt ctggactcag gcagagattt gtaacaaagg
    12301 aaacgaactg cactaatttt caccgtagca gatgattttt gcgccttgtc gctgctgcgt
    12361 gtggttggta aagtaagcgg attttctttt ccgccccagc tttcaggatt atcccttagt
    12421 atgttgaaaa aatttcgtgg catgttttcc aatgacttgt ccattgacct gggtactgcg
    12481 aataccctca tttatgtaaa aggacaaggc atcgtattga atgagccttc cgtggtggcc
    12541 attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag
    12601 cagatgctgg gccgtacgcc gggcaatatt gctgccattc gcccaatgaa agacggcgtt
    12661 atcgccgact tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc
    12721 aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg taccggttgg cgcgacccag
    12781 gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg
    12841 attgaagaac cgatggctgc cgcaattggt gctggcctgc cggtttctga agcgaccggt
    12901 tctatggtgg ttgatatcgg tggtggtacc actgaagttg ctgttatctc cttgaacggc
    12961 gtggtttact cctcttccgt gcgcattggt ggtgatcgtt tcgacgaagc tatcatcaac
    13021 tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac
    13081 gaaatcggtt cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac
    13141 ctggcagaag gtgttccacg cggttttacc ctgaactcca acgaaatcct cgaagcactg
    13201 caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg
    13261 gaactggctt ccgacatctc cgagcgcggc atggtgctca ccggtggtgg cgcactgctg
    13321 cgtaaccttg accgtttgtt aatggaagaa accggcattc cagtagttgt tgctgaagac
    13381 ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga catgcacggc
    13441 ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg gaaatgtctg tttaccctgc
    13501 ctggtctgat acgagaatac gcataactta tgaagccaat ttttagccgt ggcccgtcgc
    13561 tacagattcg ccttattctg gcggtgctgg tggcgctcgg cattattatt gccgacagcc
    13621 gcctggggac gttcagtcaa atccgtactt atatggatac cgccgtcagt cctttctact
    13681 ttgtttccaa tgctcctcgt gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg
    13741 accaactaga acttgaaaac cgggcgttac gtcaggaact gttgctgaaa aacagtgaac
    13801 tgctgatgct tggacaatac aaacaggaga acgcgcgtct gcgcgagctg ctgggttccc
    13861 cgctgcgtca ggatgagcag aaaatggtga ctcaggttat ctccacggtt aacgatcctt
    13921 atagcgatca agttgttatc gataaaggta gcgttaatgg cgtttatgaa ggccagccgg
    13981 tcatcagcga caaaggtgtt gttggtcagg tggtggccgt cgctaaactg accagtcgcg
    14041 tgctgctgat ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc
    14101 gcgtaattgc agccggtaac ggttgtacgg atgatttgca gcttgagcat ctgccagcga
    14161 atacggatat tcgtgttggt gatgtgctgg tgacctccgg tctgggcggt cgtttcccgg
    14221 aaggctatcc ggtcgcggtt gtctcttccg taaaactcga tacccagcgc gcttatactg
    14281 tgattcaggc gcgtccgact gcaggactgc aacgtttgcg ttatctgctg ctgctgtggg
    14341 gcgcggatcg taacggcgct aacccgatga cgccggaaga ggtgcatcgt gttgctaatg
    14401 aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt
    14461 tacctgaacc ggcaacgggg atcgctcagc cgactccgca gcaaccgacg acaggaaatg
    14521 cagctactgc gcctgctgcg cccacacagc ctgctgctaa tcgctctcca caaagggcta
    14581 cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag
    14641 ctatcgtagc cagggacgtt gggtaatctg gctctctttc ctcattgcgc tgttgctgca
    14701 aatcatgccc tggccggata acctgattgt tttccggcca aactgggtgt tactcatctt
    14761 gttgtattgg atcctggcct tgcctcatcg cgtaaatgtg ggcacaggtt ttgtgatggg
    14821 tgccatactg gatctgatca gcggctcgac gcttggcgta cgcgtattgg cgatgagcat
    14881 cattgcttac ctggtggcgc tgaaatacca gcttttccgc aacctcgcat tatggcagca
    14941 ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt attgttttct gggcagagtt
    15001 tttagtgatt aacgtctctt tcagaccgga agtgttctgg agtagtgtag tcaatggggt
    15061 gctctggccg tggattttct tgctgatgcg taaagtccgt cagcagtttg cagtgcaata
    15121 aaggtttcta tgacttctct gtatttagct tccggttctc cgcgtcgtca ggagttactt
    15181 gcgcaacttg gtgtgacctt tgaacgtatt gttacgggca ttgaggagca gcgtcagccg
    15241 caggagagcg cgcagcagta tgttgtgcgt ctggcgcgcg agaaagcaca ggcgggtgtc
    15301 gcgcaaacgg cgcaggatct cccggtgctg ggtgcggata ctatcgttat cctgaatgga
    15361 gaagtgctgg agaaaccgcg cgacgcagag catgcggcgc agatgttgcg caaattatcg
    15421 ggtcagaccc atcaggtgat gacggcagtg gcgttggccg acagccagca cattctcgat
    15481 tgcctggtgg tcaccgatgt gactttcaga acgttaacag acgaagacat cgcgggctat
    15541 gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg gtattcaggg gctgggtggc
    15601 tgttttgtca ggaagataaa tggcagctat cacgccgtag tcggcttacc tctggttgaa
    15661 acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga aaagggataa acatgacggc
    15721 tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg atggcggtat
    15781 tctgcaggaa attcatattg aacgtgaggc gcgacgcgga atagtaggca atatctacaa
    15841 gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct tttgtagata ttgggctgga
    15901 taaagccgcg tttcttcatg catccgatat catgccgcac accgaatgtg tggcgggtga
    15961 agaacaaaag caattcacgg tgcgcgacat ctcggaactg gttcgtcagg ggcaagatct
    16021 gatggtgcag gtggtgaaag atccgcttgg cactaaaggt gcgcgcctga ccaccgatat
    16081 cacgctgcct tctcgctatc tggtctttat gccaggggct tctcacgttg gggtttccca
    16141 acgtattgaa agcgaatcag aacgtgaacg cctgaaaaaa gtggtcgcag agtattgcga
    16201 cgagcagggc gggtttatca tccgtaccgc agcggaaggg gttggcgagg ctgaactggc
    16261 ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt atggagcgta aaaagcgccc
    16321 gcagacccgt tatcagctgt acggcgaact ggcgctggct cagcgtgttc tgcgtgattt
    16381 cgccgacgcc gaactggacc gcattcgcgt tgactcacgc ctgacttacg aagcattact
    16441 tgagttcacc tcggagtaca ttcccgagat gaccagcaag ctggagcatt acaccggacg
    16501 ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc cagcgagcgc tggaacgcaa
    16561 agtagaactg aaatccggtg gttatctcat tatcgaccag accgaagcga tgaccaccgt
    16621 ggacatcaat accggagcgt ttgtcggtca tcgcaatctg gacgacacca ttttcaatac
    16681 caatattgaa gcgacgcagg ctatcgctcg ccagttacgg ttgcgtaatc tgggcgggat
    16741 tatcattatt gatttcatcg atatgaataa tgaagatcac cgccgccgcg tgctgcactc
    16801 gctggagcag gcgttgagca aagaccgggt gaaaaccagc gttaatggtt tttcggcgct
    16861 ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa
    16921 cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga
    16981 aatcatgcgc gagattgttc gtgtccacca tgcttacgac tccgaccgtt tcctggtcta
    17041 tgcttctccg gcagtagccg aagccttgaa aggcgaagag tcacactcgc tggcggaagt
    17101 ggaaattttc gttggcaaac aggttaaagt acaaattgaa ccgctctata accaggagca
    17161 gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca tccggcaaag ggtttttgag
    17221 tcacattttt agcagacaag gagtgacggg tgaggcgatt gccggggatt ttactgctta
    17281 ctggagccgc gctcgttgtg atcgctgccc tgctggttag cggcctgcgt attgctttac
    17341 cgcatcttga cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg actggcatgc
    17401 cggtagaagc cagtcagctc tcagccagct ggcagaattt tggcccgacg cttgaagcac
    17461 acgacatccg tgcagaacta aaagatggcg gcgaattttc ggttaaacgc gttactctgg
    17521 cgctggatgt ctggcagagc ctgttacata tgcgctggca gtttcgcgac ctcactttct
    17581 ggcagctgcg ctttcgcacc aacactccta tcaccagcgg tggtagcgat gacagtctgg
    17641 aagccagtca catcagcgat ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca
    17701 gtgaagtcag tttcctgacg ccatccggtc agcgtgccga gctggcgatc ccacaactca
    17761 cctggctgaa cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc tccagcctta
    17821 ccggacagca cggcgtgatg caggtgcgca tggatttgcg cgatgatgag gggttgttaa
    17881 gcaatggtcg cgtctggcta caggcggatg acatcgacct gaagccgtgg ctcggtaaat
    17941 ggatgcagga caatattgct ctggaaacgg cacagttctc ccttgaaggc tggatgacga
    18001 tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa acagggcggt gccagctggt
    18061 tgggcgagaa gcaaacgcat acgctgtcgg tggataatct gaccgcgcat attacgcgtg
    18121 aaaatccggg ctggcagttc tctattcccg atacacggat cacgatggac ggcaaaccct
    18181 ggccgagcgg agcattgacg ctggcctgga taccggaaca ggacgttggc ggcaaagaca
    18241 ataaacgcag tgacgaactc cggattcgcg ccagtaatct ggagctggca ggcctggagg
    18301 gcatacgccc gctggccgcg aaactttcac ctgcactggg tgatgtttgg cgctccacac
    18361 aaccgagcgg caagattaac actctggcgc tggatatccc gcttcaggcg gcagacaaga
    18421 cccgttttca ggcatcgtgg agcgatctgg cctggaagca atggaaatta ttaccgggtg
    18481 cggaacactt ctccgggacg ctttccggca gcgttgaaaa tggtttgctt accgcgtcga
    18541 tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc gccactggaa atcgccgacg
    18601 gccaggcaac tataagctgg ctgaacaatg acaaaggttt ccagctggat gggcgtaata
    18661 ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt tcgttacctg caacctgcta
    18721 acgatgaacc ctggctgggt attctggctg gcatcagtac cgatgatggt tcacaagcct
    18781 ggcactattt cccggaaaac ttgatgggta aagacctggt tgattactta agtggcgcga
    18841 ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg tggcaatccg caactcttcc
    18901 cctataaaca caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg
    18961 ccttccagcc ggactggcct gcattaacta accttgatat tgaactggac tttattaacg
    19021 acggtttatg gatgaaaacc gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca
    19081 ccgcagtgat ccctgactac tcgaaagaaa aactgctgat tgacgctgac attaaaggtc
    19141 cgggtaaagc cgttggccct tactttgatg agacaccgct gaaagattct ctgggtgcga
    19201 ccctgcaaga actccagctc gacggcgatg tgaatgctcg cttacatctt gatatcccgc
    19261 tgaacggcga gctggtaacc gcgaaaggtg aagtgacgct gcgtaataac agtctgttta
    19321 tcaaaccact cgacagcacc ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg
    19381 atctgcaaag tgaaccactg acagcaagct ggtttaatca gccgttgaac gtggattttt
    19441 ccaccaaaga aggggcaaaa gcctaccagg ttgcggtgaa tctcaacggt aactggcaac
    19501 cggcgaaaac cggcgttctg cctgaagcgg tgaacgaagc attgagtggc agcgtggcgt
    19561 gggatggtaa agtgggcatt gatctgcctt atcatgctgg cgcgacctat aacgtagagc
    19621 tgaacggcga tctgaataat gtgagtagtc acttaccttc accgttagcc aaacctgcgg
    19681 gtgaaccact agcggtaaac gttaaggttg atggcaatct caacagcttt gaattaaccg
    19741 gacaggctgg tgcggataac catttcaata gccgctggtt gcttggtcaa aagctgacgc
    19801 tcgatcgtgc tatttgggcg gcagacagta aaacgctccc gccgttgccg gaacaaagtg
    19861 gcgttgaact caatatgccg ccgatgaatg gtgccgagtg gctggccctg ttccagaaag
    19921 gcgctgcgga gagtgtcggt ggtgcagcga gtttcccaca atacataacg ttacgtacgc
    19981 ctatgttgtc actgggaaat cagcaatgga ataacctgag tattgtttcg caaccgacgg
    20041 caaatggcac cctggttgag gcgcaagggc gtgaaatcaa cgccacgcta gcgatgcgta
    20101 ataacgcgcc gtggctggcg aatatcaaat atctttatta caacccgagc gtggcgaaaa
    20161 ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac ggagcgcatt aacttccgtg
    20221 gctggccgga cgcacaaata cgatgcacag agtgctggtt ctgggggcaa aaattcggtc
    20281 gcattgacag tgatatcacc atttctggcg atacgttaac gctgaccaat ggactgattg
    20341 atactggttt ctcgcggctt actgccgatg gtgaatgggt taataatccg gggaatgaac
    20401 gtacctcgct gaaaggaaaa ctgcgcgggc agaaaattga tgccgccgca gaattttttg
    20461 gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta cactggcgca
    20521 aagcaccctg gcagccagat gaagcgacgt tgaatggcat cattcatact caactgggta
    20581 aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc ttattgagcg
    20641 tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc gaagggttct
    20701 attttgactc cattcgcagc accgcgtgga ttaaagacgg ggttatgcac accgacgaca
    20761 cgctggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat ctggtacgtc
    20821 gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg ggcgtggctg
    20881 cggcttttgc ggttaacccc attgttggcg cggcagtttt tgccgctagt aaagtgctgg
    20941 ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg ctggacgatc
    21001 cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga tttgacgagg
    21061 gcgcgtaatt gccccaatct catatgataa tcgttgccaa aggccaacga gccagaacat
    21121 aaccgtaggc cggatagggc gttcatgccg catccggcag ccgtaaaaaa tcctctactg
    21181 cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac aattgctagc
    21241 ggcgaacagc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg
    21301 ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg ttttagaaga
    21361 ccgcattatt aaagatggtt cttacaacat cgatcagggc gttggcgtgc gtgcaatcag
    21421 cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc tggaacagag
    21481 tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac agacgctggg
    21541 cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca tgagccgtga
    21601 agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg acaagcgcgt
    21661 acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg cggccaccga
    21721 cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg ttctggtcga
    21781 agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg gttatgagtt
    21841 cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag aagcagtgcg
    21901 tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact
    21961 tggcgcaggt tggccgggcg tgctgttgca tgaagcggta ggtcacggtc tggaaggcga
    22021 cttcaaccgc cgcggcactt cagtatttag tgggcaggtc ggggagctgg tggcttcaga
    22081 actgtgtacc gtggttgatg acggcacgat ggtcgatcgc cgtggttcgg tggcgattga
    22141 tgacgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc tgaaaggcta
    22201 catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca acggtcgccg
    22261 tgaatcctac gcccatctgc ccatgccgcg tatgactaac acctatatgc tgccgggtaa
    22321 atcgaccccg caggaaatta ttgaatccgt tgagtacggt atctatgcac cgaactttgg
    22381 tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag aagcatatct
    22441 gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg gttccggtat
    22501 cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata acggcgtggg
    22561 tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt
    22621 tgataacctg actgttggcg gtactgcgta ataatttcat tattttcaga aggataatta
    22681 atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga ggggaacccg
    22741 ttggcacagg tttgtagaac gtaacagtac aatatgaatt acttctcttt cccgcgcccg
    22801 tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga gttgatcacg
    22861 acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac cgggcgtgga
    22921 tctgactgat aacgcggcag caggatctcc agctcaccgc gattgatctc gttgatcacc
    22981 cacatcagcg gcacgtaggc gatcccggca cccgccgtca gccagcgcac cagcgtcatc
    23041 ggatcattag tcacaaatct tccttgtggg atcaggcgag tcgagatccc ttccggtgcg
    23101 atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg actactcaaa
    23161 tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc gcacaccacc
    23221 atcggcatcg cgcccagacg gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg
    23281 atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt aaccagattg
    23341 acgctcaaac ctgggtattc tttcagcatt tttgctgtca gcccggcgag aacattttgt
    23401 gccatagttg aagaacagcc aatgcgcagc gtcccgatgg gggtgttatt gaaggcatac
    23461 agttgctcat gaacatcctg cacttcatga agcatccgac ggcagccctg gtagtaaatt
    23521 ctgccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt tacctgcaac
    23581 tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg tagctgtctg
    23641 gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat gcgttttagt
    23701 cgttccatta ttcactctga cttaaaagtg atttagatca cataatatag ataacagcat
    23761 aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg cccggctttc
    23821 catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc tctttacaac
    23881 caaggtcaac atgagtctgt ttcccgttat cgtggtgttt ggactgtcct tcccaccgat
    23941 attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg tacttgtgcc
    24001 aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc tctattgctg
    24061 cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca ctaataagaa
    24121 aattctcccg tacggccatc acggtcgtat tagtcattct agccttcatc gcaattttta
    24181 atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt agcgctgacg
    24241 tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt catgataacc
    24301 agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat caaaaggcgc
    24361 ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag aaacgccagg
    24421 aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg
    24481 ccaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg acccgcgatc
    24541 tggcaaaact ggatcttgaa cgcacggtga tccgcgcgcc agcagatggc tgggtgacca
    24601 acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt gcgctggtga
    24661 aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa ggggggcgtc
    24721 cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa gggactgttg
    24781 atagtgttgc cgcaggggtc accaacgcca gcagcacgcg tgacgacaaa gggatggcga
    24841 ctatagactc taatcttgaa tgggtgcgtc ttgcgcaacg tgttccggtt cgtattcgtc
    24901 tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg gtggtcactg
    24961 gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc catcgcctgc
    25021 gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata ttcgctttgc
    25081 ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact tccagctgga
    25141 aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg cctttgctgc
    25201 gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca tcatcggcac
    25261 atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg caccattatt
    25321 gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct cgctggtacg
    25381 aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca ttgtgatcac
    25441 cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt
    25501 tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat cgatcaaaca
    25561 agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg
    25621 tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg tgcgacgcac
    25681 cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct gggcgcgggc
    25741 caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc aatcctgcga
    25801 aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc gcgaattttt
    25861 tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt
    25921 tatcgcctgg accggggaac gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc
    25981 aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca acgccaccga
    26041 agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac gtcatcatgc
    26101 aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcttt tctggctgtg
    26161 gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga cgtcactggc
    26221 aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga cgctggccgc
    26281 gctgccgtta gggctgctct actttttggt gattatccct aatacccaac agagcatgtt
    26341 gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag tacagaaacg
    26401 gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc tggataaccc
    26461 gatgactttc catttcagtc agtttctcga cagcgcatta gggcaaatcg tcggctgtgt
    26521 gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt
    26581 actgcttaat cagtttgttt ttgccgctgt ttccgcgatg actaccaatg tggcacgtcg
    26641 taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga ataagttccc
    26701 aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg
    26761 tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa tgcgccgcac
    26821 agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact ttggccagtt
    26881 gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc caccgcaggt
    26941 gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg cgttgaccga
    27001 tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac ttagcgatgc
    27061 acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat acctttgatt
    27121 ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc tttggtctgg
    27181 ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac gatgtcctgc
    27241 cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg
    27301 cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg cgttttaatg
    27361 ttcgtcatta gcgcaaaaaa aagcccccga accgggggca atatcgtcgg acaagacgat
    27421 gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac gagcttcagt
    27481 aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt cacgcatatc
    27541 cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac gattttgtgc
    27601 ttgtgcagta tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct
    27661 taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat cttttagctg
    27721 ggcctttgtt tcgtgaccca tatcacaaaa tcaagcatac actaattatt gaactaatta
    27781 ataccacgc
//
LOCUS       DAZPLM010000034        26917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPLM010000034 DAZPLM010000000
VERSION     DAZPLM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.034"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHVC7_001240"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHVC7_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR2115344.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHVC7_001241"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHVC7_001241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR2115345.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHVC7_001242"
     CDS             complement(2203..3618)
                     /locus_tag="ACHVC7_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115346.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHVC7_001243"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHVC7_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR2115347.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHVC7_001244"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHVC7_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR2115348.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHVC7_001245"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHVC7_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR2115349.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHVC7_001246"
     CDS             11339..11395
                     /locus_tag="ACHVC7_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2115350.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHVC7_001247"
     CDS             11666..11722
                     /locus_tag="ACHVC7_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2115351.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHVC7_001248"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHVC7_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR2115352.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHVC7_001249"
     CDS             12275..12934
                     /locus_tag="ACHVC7_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR2115353.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..13692
                     /gene="pphC"
                     /locus_tag="ACHVC7_001250"
     CDS             12931..13692
                     /gene="pphC"
                     /locus_tag="ACHVC7_001250"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR2115354.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIHQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            13689..15629
                     /gene="yegI"
                     /locus_tag="ACHVC7_001251"
     CDS             13689..15629
                     /gene="yegI"
                     /locus_tag="ACHVC7_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR2115355.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHVC7_001252"
     CDS             complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHVC7_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2115356.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            17128..17976
                     /gene="alkA"
                     /locus_tag="ACHVC7_001253"
     CDS             17128..17976
                     /gene="alkA"
                     /locus_tag="ACHVC7_001253"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR2115357.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(18086..21403)
                     /locus_tag="ACHVC7_001254"
     CDS             complement(18086..21403)
                     /locus_tag="ACHVC7_001254"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR2115358.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            21721..22362
                     /gene="udk"
                     /locus_tag="ACHVC7_001255"
     CDS             21721..22362
                     /gene="udk"
                     /locus_tag="ACHVC7_001255"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR2115359.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            22454..23035
                     /gene="dcd"
                     /locus_tag="ACHVC7_001256"
     CDS             22454..23035
                     /gene="dcd"
                     /locus_tag="ACHVC7_001256"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR2115360.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            23057..24910
                     /gene="asmA"
                     /locus_tag="ACHVC7_001257"
     CDS             23057..24910
                     /gene="asmA"
                     /locus_tag="ACHVC7_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR2115361.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(25184..26767)
                     /locus_tag="ACHVC7_001258"
     CDS             complement(25184..26767)
                     /locus_tag="ACHVC7_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR2115362.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         6392 a         7194 c         7233 g         6098 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc cagggggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgcaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgactgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgcagctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcatc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct catgtgcctt tctttacccc gtttttcaat
    13561 ggactggcgt cagcaacgca ggaacaactc gatttattac ctgaattgtt aaagcagttt
    13621 ttgtccagcc cggcggtaaa cgagcgtacg gatgatgata aaacgttggc actggcgctg
    13681 tggttaccat gaaacccact ttatatactg ctactggtga gtgcgttacg ccaggccgtg
    13741 aactgggcaa aggcggtgaa ggcgcggttt atgatatcaa tgagtttgtc gatagcgtcg
    13801 ccaagattta tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg
    13861 cgacagctga cgcgcaattg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg
    13921 gacgaggcgg aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc
    13981 atatgatcta tagcccggca catcgtcgtc aacgttaccc tcattgtgcg tgggattttc
    14041 tactctatgt tgcgcgcaat attgcttcat cttttgctac ggttcacgag cacgggcacg
    14101 tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg cgacagcaaa gtggtgttga
    14161 ttgatagcga ctcctttcag attaacgcca atggcacact gcatttatgc gaagtcggcg
    14221 tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc atttgtcggc tttgaacgca
    14281 cagaaaatca cgataatttt ggccttgcgt tgctgatttt tcacgtcttg tttggtggcc
    14341 ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg taatgcgctg gagacggata
    14401 ttacccattt ccgttatgcc tacgcgtcag ataatcagcg acgtggttta aaaccgccgc
    14461 cacgatcgat tccgctatcg atgttaccga gtgatgttga agccatgttt cagcaggcgt
    14521 ttacggaaag tggcgtggca accgggcgtc cgacggctaa agcgtgggta tcagcactgg
    14581 attctctacg ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt tatcccggtc
    14641 atttggctga ctgcccatgg tgtgcgctgg ataatcaagg tgttatctat tttattgatc
    14701 tcggcgaaga ggtcattacc accggcgggg attttgtgct ggcaaaagtc tgggcgatgg
    14761 tgatggcgtc agtagcaccg ccagcattgc aactgccatt acccgatcat ttccaaccga
    14821 ctggcaggcc gcttccttta ggcctgttac ggcgcgaata catcattctg cttgagatcg
    14881 cactgtcagc attatcgctg ctgctttgcg gccttcaggc agaaccgcgt tatattattt
    14941 tggttcctgt gctggcggct atctggatta ttggcagttt gacaagcaaa gcgtacaaag
    15001 cagaagttca gcaacgccgt gaggcattta atcgtgcgaa aatggactat gaccatttag
    15061 tccgccagat ccagcaggta ggcggcctgg aaggttttat cgccaaacgg acgatgctcg
    15121 aaaaaatgaa ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggcagcac
    15181 ttcatgacac tgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg
    15241 ttgcctctat tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg
    15301 aaacagccgc agatgttacc cgtcgtggcg ttaagcaagt gaaagggttt ggcgaccatc
    15361 tgacccaggc ggttatcgac tggaaagcga actgtgaacg ccgttttgtg ttcaggccga
    15421 atgaagcgat aacgccagca gacagacaag cggtaatggc gaaaatgacc gccaaacgac
    15481 atcggctgga atcggcgttg acagtcggcg cgacagagtt gcagcgattc cgccttcatg
    15541 ctccagcacg gaccatgccg ttgatggaac cgttacgtca ggcagcagaa aaactggctc
    15601 aggcgcaggc agatttaagc cgctgctgag ccatttttca attaacgaaa caccacttcc
    15661 gcccagcgtg ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg
    15721 cccggcaact gttctgccag cgctttttta atctgcggag aacgggcgct accgccggtc
    15781 aggtagataa cgtccggttt ttcctgggcg ttatccaggg ccagttgcac ctgttccaga
    15841 atccgcgcca gtggctggct gagggcgctt tccagccctc gctggctaat cagcgtagcc
    15901 agttcattgc tgataaacgg cagtgaggcg cgggtttccg ctacgcttga aagagcaatt
    15961 ttgcactctt ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt
    16021 aacagggcca ctttttccgg ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg
    16081 ttggcactac tgtagaaatc actctgcgca ggtacgtcgt tgatggcaac cgcattccac
    16141 cacggcagga tcggcagggc gatgcctttt tcggtttcgc cacccatacc cagcagtggc
    16201 atcaggtttt taaacgccag ggcgatatcc agatcgttac cgccaatacg acaaccactg
    16261 tgacccagca gactggcttc acgatcgaga cgcgaacgcc actgcggccc catcagcagc
    16321 aatgaacagt cagtcgtacc accgccgata tccaccacca gcacccgttt ttcttcctgc
    16381 aaggtggctt cgtaatccag cccagccgcg atcggctcgt actggaatac cacgtccctg
    16441 aatccggcac gcttcgccgc gcgttccaga atctcttgcg cctgggcgtt tgcttcatcg
    16501 ccgcccagcc cctggaagtt gatcgggcga ccaatcaccg cctgagtaat cgcttctggc
    16561 agctgcgcct gcgcctgctg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac
    16621 agcgctacct gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca
    16681 aaccacactt cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg
    16741 cttttcgccg taacatcgat atcttcttcg cggttataac gaatcgcccg acgtaacagc
    16801 gcctgcgttt catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttacc
    16861 gattcacgcg ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttc
    16921 agtaattgcg gtttaccgtc acgcatgacc gccactgaac agtttgctgt accgtaatca
    16981 aaaccaataa acacgaatta atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa
    17041 tgttgctgtc gcgacaaccg gaatatgaaa gcaaagcgta acgtctgaat aacgtttatg
    17101 ctgaaagcgg attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac
    17161 tggtcgtgga tgttgggatt tctcgccgcc cgtgcggtga gcggtgtgga aacggtcgcg
    17221 gacaattatt atgcccgtag tctggcggtg ggcgaatatc gcggcgtagt gactgctatt
    17281 ccggatatag cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc
    17341 gcagagtgtc tggcgaaaat gagccgcctg cttgatctgc aatgtaaccc gcagattgtt
    17401 aacggtgcgt tgggaaagtt aggcgcgggg cggcccggat tgcgtttacc cggctgtatt
    17461 gatgcttttg agcagggtgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg
    17521 gcaaaattga ctgccaaagt ggtacagctt tatggcgaac ggctggatga ttttccggag
    17581 tatatctgct tccccacgcc tcagcggctg gcagcagccg acccgcaggc attaaaagcg
    17641 ttaggcatgc cgttgaaacg ggcagaggca ctgattcatc tggcaaatgc ggcgctggag
    17701 ggcagcttac caatgacaat tccgggcgat gtggagcagg cgatgaaaac gctgcaaact
    17761 tttccgggta tcgggcgctg gacggcgaat tattttgctt tgcgtggctg gcaggcgaaa
    17821 gatgtttttc tgccggatga ttatctgatt aaacggcgat ttccgggaat gacaccggca
    17881 caaatccgcc gttatgccga gcgctggaag ccctggcgtt cttatgcgct gttgcatatc
    17941 tggtatacgg aaggctggca accagacgaa gcatgacgtt ggtcgccgga tgcggtgcga
    18001 gcgccttatc cggtctgggg aatgagcgat ttgtaggcat gataagacgc gttagcgtcg
    18061 catcaggcaa tgggcaaacg ctctctcagt taatcgcgaa ataactgcta ttcaccagca
    18121 aatccagcgg ttgggcatcg gcaatcacat caccataaat cagatcgacg ccgatgccag
    18181 aaagcgtatc catcactaag ggtaaaacga ctggcccggc gatggttttc atcccgagtc
    18241 gctgagcgtg cccctgaata atcgtaatca gcatctcatc catcaaatta ccctgcacgt
    18301 tggcgcataa ctcaccatca agtagcaggt aatctgccat attcgctttc agcgagttga
    18361 agatttgcag atcgcggccc acctggctga gcactatccg acatcccgcc agctgcagtt
    18421 tttgcacgct ttctgcgtga tctaaaatcg cttcagccgg aataatcaga tgtaataacc
    18481 gtggtggtag agggctattt tccagctgct caagcagatc attcaccagc gtggcgctac
    18541 tcaaaccggc aacggaaagg gggagggcga tgcttatgcc tttactggca accgctttgg
    18601 cggcctgctg gaaaaattcg tggaataccc gtcggtcaag agcatggcta agtgccggat
    18661 cgctgaagct acgacgaaat gtttgttcat caataatttc gccttcgcaa ctccagagct
    18721 taagcgaaat cagccacaaa ttacgcgctt ccgggatccg cggcgaagcg acaccgtggg
    18781 cgatcatcat caactgattc tctttaatca tccgccactg ttcatcaagc gacatcgccg
    18841 cccgttcgct atgtgcggca gcttgctgtg gttcgtaaac cgtcacccgg ccccgtccac
    18901 catttttgga ggcataacag gcgatatcag cctgcgacat cacttctgcc gcctgatgat
    18961 tgttgtcatc gatcaaggta atcccggcgc tggcacctac ccgatgtacc cggccttccc
    19021 atataaagtg atagtcattc acagcactga taatacgtgt agcgataaaa cgagcgcttt
    19081 caacattgca gtctggcagc agcagaccaa attcatcacc gccgagccgc gccagcacgt
    19141 cactggagcg cagcatactc agcatcaacg acgccagttc gcgcagcaaa gcgtcgcccg
    19201 ccgcatgccc ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg
    19261 catgtcgctg atgcgtactg tttaccgttt gcagcaggat acgcagttgt ttctcaaaac
    19321 tggcgcggtt ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca
    19381 gcattttgcg tgattcggtg acgtcctgaa tcaccagaac cgaaccaata ttgctgccgt
    19441 ccagagtact taacggcgta atactgtaat gcacgtcgta actgccgccg ctccgacagt
    19501 gcaacaccac atcttgttcg attgcggaac gtgaggtgtc ggcactgtaa atgttctcca
    19561 ttaatggtcc gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg
    19621 cttcttcctg cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca
    19681 tcgccatatc aatacagacc acggcttcac cgatggaatc aagcgtaatg tgcaggcgct
    19741 ctttttcctg aaacaatgcc tcgttaagct gtttcacttc ggtcatatcc atattgatgc
    19801 cgaggagacg ttcgacttcg ccttctttat tcagtacccg gttggcgagg gcgcggatat
    19861 ggcgaatacc gtcttttacg gtaatgcgaa attccagttt aaagggcgag cgtgattgca
    19921 acgaatcacg aatcactttt tcggcatgct ggcgatcttc cggcagcacg cactcgtacc
    19981 acacctgcca gttcggtttg atatgtggag gaatttcata cagctcgaac atccgcttat
    20041 cccagctaaa aacattcggc ttcaactccc actcccagat gccaatcccg cccgcttcgt
    20101 tagccagcgt gatgcgctcc atcagttgct gattgacctg ttcggtgcgt tttagctcgt
    20161 taatgtcttc aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg
    20221 agacggcaag caacgcccag acaacatcgc cattgcggtt gtagtagcgt ttttccattg
    20281 aataggtgtt tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat
    20341 cctccggcca ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtacccga
    20401 gaaactggca gagcgctttg ttggtttgca gccattgtcc ctcggtgccc actaacgcca
    20461 taccgatagc ggaatattcc atcgcgttcc gaaagcgggt ttcgctttcg gaaatgtgtt
    20521 tgcgttccgc acgaaaggca tacatcacca tggtcatgat gttggcgggc agcaggatca
    20581 gcaaaaacgg tagccacggc atatggctca tcaggtacgt acgcggcgta gcaagcaggg
    20641 agggatccgc ggccatcatc agcgacacca tcatcaccgt ggtaaggaag atcaaaaagg
    20701 cttccattcg tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag
    20761 gccacggcag atacagcatc gaaagccagc ttaacgtcag tgtgatggct aacgtgagca
    20821 gcgactcaaa aagcaaccgt gggttgcgat ggcgcagcag atagtgtggt ttaaataaca
    20881 atcccagcgg caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg
    20941 ccctgagagg atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg
    21001 gaacaatggc gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat
    21061 accacggcag caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg
    21121 tcgtccaggt catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc
    21181 ccagcgaaca gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca
    21241 ccatcatgat ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct
    21301 caagcgagaa aagggtaaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg
    21361 ggtggggcag ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa
    21421 gaccaggtcg ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata
    21481 tgcgaattgc tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt
    21541 tttaccttac ataaaactga tcaacgtaat ttgccaccgg gaattctttg tcaacaatta
    21601 actctgagcg cgcggcaacg ctattcgact ggtatcagac ggatgaaatc cctataattg
    21661 ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat
    21721 atgactgatc agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc
    21781 aagagtctta ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac
    21841 atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa
    21901 cgcgttaaga ccaactacga ccatcccagc gcgatggatc acagcctgct gcttgagcat
    21961 ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat
    22021 acgcgtatga aagaaacggt gacggttgag ccgaagaagg tcatcattct cgaaggcatt
    22081 ttgttgctga cggatgcgcg tttgcgtgac gaacttaact tctccatttt cgttgatacc
    22141 ccgctggata tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca
    22201 atggattccg tgatggcgca atatcaaaaa accgtgcgcc cgatgttcct gcaattcatt
    22261 gagccttcta aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcatcgcg
    22321 atcgatatat tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac
    22381 cgttcagtga taacctggta tgcccttgac gtaattggcg ttaagggagt gatgcgcgaa
    22441 aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg
    22501 tttgtcgatc aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtacg
    22561 cctgggcaat aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg
    22621 tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaga tcgttctcga
    22681 cgagggcgag gcgttctatc ttcacccagg agagctggcg ctggcggtga cgctggagtc
    22741 ggtgacgctg ccagccgatc tggtgggctg gctggacggg cgttcctctt tggcgcgtct
    22801 ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg ggctggtctg gttgcattgt
    22861 gctggagttc tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg
    22921 tgcgctgagc tttgagccgc tttccggccc ggcggcgcga ccttacaacc gccgtgaaga
    22981 tgcgaaatat cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag
    23041 gcccattgag gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct
    23101 ggtggccggg ttatctgcgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat
    23161 ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg
    23221 gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc
    23281 aagccagcca ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact
    23341 gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc
    23401 gcagacggaa gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc
    23461 ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag
    23521 cgtgctggtg ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca
    23581 aatggaacaa gatccccaac atcgtggctc atttgagttc tccgggcggg ttaatcgcga
    23641 tcagcgcgat ctcacaatat cccttaacgg tacggtggat gcttctgatt atccgcatga
    23701 tttaacggcg gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca
    23761 aggtattcag gggcagggga gtttccaggc ccagtggcag gagtcacata aacgcctttc
    23821 atttaaccaa atcagcttga ccgccaatga cagtacgctg agcgggcaag cacaggtcac
    23881 gctgacagag aaaccggaat ggcagctgag actgcaattc ccgcaactga atcttgacaa
    23941 cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt gccgcgcagc aggggcagag
    24001 ccaatcaacg ttgccgcgcc cggtcatttc ttcgcgtatt gatgaaccgg cctatcaggg
    24061 actgcaaggc tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat
    24121 gaattttaca gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg aaattactca
    24181 actgcagggc aaacttaacg gtggacaggt ttcactgccg ggcacgctgg acgcgacatc
    24241 aataaatccg cggataaact tccagccacg gctggaaaac gttgagattg gtaccattct
    24301 gaaggcgttt aactatccga tttcgttgac cggaaaaatg tcactggctg gtgatttctc
    24361 cggtgctgac atagatgccg acgcattccg ccacaactgg caaggacagg cacatgtcga
    24421 aatgaccgac acgcgcatgg aagggatgaa cttccagcag atgattcagc aagcggtaga
    24481 gcgtaatggc ggtgatgtga aggccgctga aaactttgat aacgtgacgc ggcttgaccg
    24541 ctttaccacc gatttgacgt tgaaggatgg cgtcgtgacg ttaaacgaca tgcaaggtca
    24601 atcgcctgtg ctggcgctga caggggaagg catgttgaat ctggcagatc aaacctgcga
    24661 tacccagttt gatattcggg tcgtgggtgg ctggaacggg gaaagtaaac tgattgattt
    24721 cctgaaagaa acgccagtac cgctgcgggt ttatggcaac tggcagcaac tcaattacag
    24781 cctgcaagtg gatcagttac tgcgcaaaca tctacaggac gaagcgaaac gtcgcctgaa
    24841 tgactgggcc gagcggaata aagattcccg caatggcaag gatgtgaaga agttgctgga
    24901 gaagatgtag gttaccaggt gaccagtcct gtaggcctga taagacgcgt aaagcgtcgc
    24961 atcaggcgtt cgtggcggca tcattatatt ctgaatatgt aagatcgaag gctgttccgt
    25021 tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg tgaacgtgcc agggtggctc
    25081 aatcgccacc accctggcaa cccgggctcc cggcggtaaa tcgccgctgg cgcggttccc
    25141 tcatcttatt cctttcggct attgggtaaa atgaccgata ccatcacacc tcgtactcca
    25201 actcgccatc cttatgcagc ggaataatct gcaccttctg cacgcgatgg ctttccacct
    25261 gcaacgtttt aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct
    25321 gtaaatattc catcaacagc ccggcaatgg tgtgatattc acgtttttca tccagcggca
    25381 gcggcacata ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt
    25441 ccgcattctt ctgaatatca tggcgggcgt cgatctcttc cacttcgttc ggtaagttac
    25501 cgacaatggt ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat
    25561 ccaccacaaa agcaaagtgc gtgcgggcat tacggaactg ctccagggca ggtaacaacg
    25621 gcaaggtttc cgggaacacc agcggctggc gaatcaacac ccgcaggttg agcggttcgc
    25681 cgcgcagaga ctgttgtagc aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt
    25741 catcaccgtc ggtaaccacc agccgcgtat gctgatttcg ctccagcaac tggcggattt
    25801 cttcttccgg cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc
    25861 tgacggtacg ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa
    25921 agatctgctg gttaccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat
    25981 cttctttttg cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgcagcgtct
    26041 ggttagcgga aagaaaacgt cgccggttaa agatagccaa ctgattgagc gcctcgatca
    26101 tcaccgagaa accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt
    26161 ctgccaccag gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatcgtcg
    26221 ggtgactgtt aacaaattgc gttaacggct tgctggccat caacatcagg ctgatagcga
    26281 taaccacggc ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcactg
    26341 agtcgagtga gaaaatggcg tccagtacca caatttgcgt caccacgccc cagaacttcg
    26401 cacctttgcg ttgtgtggga ttattgctgt ctttcccttc cagccgttcg ttcagctcca
    26461 tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaagtcg cgggcgctaa
    26521 aggtaaacga gcggaagctg aacagcggtt gagtcagggt gaccagccag gagattgacg
    26581 ccagcagtaa caggcgcatt aacatcgcca gcagcagccc ggtaacccgt gcgcggtcgc
    26641 gctgcctcgg cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga
    26701 ggaccagttc gatcacaatc agcgtgatta acccggccca gatagacgga tcggcaatcc
    26761 attccatagt aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga
    26821 taaatcgggg caaaaatgca atgttgcagc actatttatc tcatattatt tgagggtaat
    26881 aacctcacat tatccctgaa ttaaaagtgg taataat
//
LOCUS       DAZPLM010000035        26567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPLM010000035 DAZPLM010000000
VERSION     DAZPLM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.035"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            62..793
                     /gene="artJ"
                     /locus_tag="ACHVC7_001259"
     CDS             62..793
                     /gene="artJ"
                     /locus_tag="ACHVC7_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR2115363.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(968..2095)
                     /gene="rlmC"
                     /locus_tag="ACHVC7_001260"
     CDS             complement(968..2095)
                     /gene="rlmC"
                     /locus_tag="ACHVC7_001260"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR2115364.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2136..2624)
                     /locus_tag="ACHVC7_001261"
     CDS             complement(2136..2624)
                     /locus_tag="ACHVC7_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR2115365.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2684..3529)
                     /gene="potI"
                     /locus_tag="ACHVC7_001262"
     CDS             complement(2684..3529)
                     /gene="potI"
                     /locus_tag="ACHVC7_001262"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR2115366.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3526..4479)
                     /gene="potH"
                     /locus_tag="ACHVC7_001263"
     CDS             complement(3526..4479)
                     /gene="potH"
                     /locus_tag="ACHVC7_001263"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR2115367.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4489..5622)
                     /gene="potG"
                     /locus_tag="ACHVC7_001264"
     CDS             complement(4489..5622)
                     /gene="potG"
                     /locus_tag="ACHVC7_001264"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR2115368.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5717..6829)
                     /gene="potF"
                     /locus_tag="ACHVC7_001265"
     CDS             complement(5717..6829)
                     /gene="potF"
                     /locus_tag="ACHVC7_001265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR2115369.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7180..7656)
                     /locus_tag="ACHVC7_001266"
     CDS             complement(7180..7656)
                     /locus_tag="ACHVC7_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR2115370.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7744..8646)
                     /gene="rimK"
                     /locus_tag="ACHVC7_001267"
     CDS             complement(7744..8646)
                     /gene="rimK"
                     /locus_tag="ACHVC7_001267"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR2115371.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8707..9429)
                     /gene="nfsA"
                     /locus_tag="ACHVC7_001268"
     CDS             complement(8707..9429)
                     /gene="nfsA"
                     /locus_tag="ACHVC7_001268"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR2115372.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9413..9700)
                     /locus_tag="ACHVC7_001269"
     CDS             complement(9413..9700)
                     /locus_tag="ACHVC7_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR2115373.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9860..10117
                     /gene="grxA"
                     /locus_tag="ACHVC7_001270"
     CDS             9860..10117
                     /gene="grxA"
                     /locus_tag="ACHVC7_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR2115374.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10147..10524)
                     /locus_tag="ACHVC7_001271"
     CDS             complement(10147..10524)
                     /locus_tag="ACHVC7_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR2115375.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10794..12479
                     /locus_tag="ACHVC7_001272"
     CDS             10794..12479
                     /locus_tag="ACHVC7_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR2115376.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12654..13190)
                     /gene="rcdA"
                     /locus_tag="ACHVC7_001273"
     CDS             complement(12654..13190)
                     /gene="rcdA"
                     /locus_tag="ACHVC7_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR2115377.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13274..14482
                     /locus_tag="ACHVC7_001274"
     CDS             13274..14482
                     /locus_tag="ACHVC7_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115378.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            14482..15297
                     /gene="ybjI"
                     /locus_tag="ACHVC7_001275"
     CDS             14482..15297
                     /gene="ybjI"
                     /locus_tag="ACHVC7_001275"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR2115379.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15383..15667
                     /gene="ybjH"
                     /locus_tag="ACHVC7_001276"
     CDS             15383..15667
                     /gene="ybjH"
                     /locus_tag="ACHVC7_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR2115380.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15708..16935)
                     /gene="mdfA"
                     /locus_tag="ACHVC7_001277"
                     /pseudo
     CDS             complement(15708..16935)
                     /gene="mdfA"
                     /locus_tag="ACHVC7_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            17220..17816
                     /gene="ybjG"
                     /locus_tag="ACHVC7_001278"
     CDS             17220..17816
                     /gene="ybjG"
                     /locus_tag="ACHVC7_001278"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR2115381.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            17874..18632
                     /gene="deoR"
                     /locus_tag="ACHVC7_001279"
     CDS             17874..18632
                     /gene="deoR"
                     /locus_tag="ACHVC7_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR2115382.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18679..19881)
                     /gene="dacC"
                     /locus_tag="ACHVC7_001280"
     CDS             complement(18679..19881)
                     /gene="dacC"
                     /locus_tag="ACHVC7_001280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR2115383.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20128..20753
                     /gene="gstB"
                     /locus_tag="ACHVC7_001281"
                     /pseudo
     CDS             20128..20753
                     /gene="gstB"
                     /locus_tag="ACHVC7_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(20750..21865)
                     /gene="yliI"
                     /locus_tag="ACHVC7_001282"
     CDS             complement(20750..21865)
                     /gene="yliI"
                     /locus_tag="ACHVC7_001282"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR2115384.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21976..22359)
                     /gene="bssR"
                     /locus_tag="ACHVC7_001283"
     CDS             complement(21976..22359)
                     /gene="bssR"
                     /locus_tag="ACHVC7_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR2115385.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22572..23897
                     /gene="rimO"
                     /locus_tag="ACHVC7_001284"
     CDS             22572..23897
                     /gene="rimO"
                     /locus_tag="ACHVC7_001284"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR2115386.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23944..25265)
                     /locus_tag="ACHVC7_001285"
                     /pseudo
     CDS             complement(23944..25265)
                     /locus_tag="ACHVC7_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(25273..>26567)
                     /locus_tag="ACHVC7_001286"
     CDS             complement(25273..>26567)
                     /locus_tag="ACHVC7_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR2115387.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         6737 a         6901 c         6397 g         6532 t
ORIGIN      
        1 ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg ggagttccat
       61 catgaaaaag ttagttcttg ccgctttact tgcttccttt actttcggtg cttctgccgc
      121 agagaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta taggtgctaa
      181 taatgagatt gtcggctttg atatcgatct ggcaaaagcc ttgtgcaaac aaatgcaggc
      241 agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga aattcagaaa
      301 atatgactcc gtaatctccg gtatggatat cacgcccgag cgtagcaagc aggtatcgtt
      361 taccacgccc tactatgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac
      421 gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc accagaaata
      481 tattcaggat cagcaccccg aagtgaaaac tgtctcctat gacagttatc agaatgcctt
      541 tatcgatctg aaaaatggtc gtattgatgg ggtatttggt gacacagcgg tggtaaacga
      601 atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg atccgcaata
      661 cttcggcacc ggtctgggca tcgctgtacg tccggataac aaagctctgc tggaaaaact
      721 gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca atgaccagtg
      781 gttcccgcag taattgagac tgatgacaaa agtaagattg cctgatgcgc tgcgcttatc
      841 tggcccacat caactctgca atgtattgat ttagcatgat tttgtaggcc ggataaggcg
      901 tacacgccgc atccggcatg aacaaagcgc acctgatcag caatctgaag ccgcacctgc
      961 ggctttttta ttgcttcacc agcagcgtca tcacttcata gtgcgcggtg tgcgggaaca
     1021 tatcaaaaag ctgtacccgt tcgatgcggt aacctggcag ttcgcggata tctttcgcca
     1081 tggtttgggc gttacagctg gagtagataa taaaacgcgg tgccatcgtt gagagataat
     1141 cacacagcgg tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc tccggcacat
     1201 ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg aaattgcaaa cgcgttaagc
     1261 ccagttcagc ggctgactgc tttgcacagg caatggcctc tgatgcaatt tcgatcccgg
     1321 ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc acccacaccg cagaacagat
     1381 cccacatatg tttaactggc agctgccgta cccagtcgcg cgcggtagcg tacagctggc
     1441 tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc ggtacgtcat
     1501 taaaacgctc tgccagcgcc tgttgttcag tcaggtagat ctccgtctcc ccttccataa
     1561 tcgccatatg taccggctga atattgacgg taataacttt cagttgcggt agttgttcct
     1621 gtaaccacgg cagcgcctta cgcagttgtg ccagtttggt ttcagaacgc agtacaaagc
     1681 gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc agttcgccac
     1741 gcttacgcgc cacgttgtag ggcgttaacc ccgcacgggc gataaacggt tttagcgccg
     1801 caaaaacggg cgcaaaagag gcaggataaa gcgggcagtc acaaaggtct tccggcgtgc
     1861 catcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc accattttgg
     1921 ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc tcaaccggaa
     1981 aatcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct ggaatcggct
     2041 gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac tgcatacatt
     2101 gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa gaaccgccga
     2161 ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt ctgcagcatc
     2221 aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc cggataaccg
     2281 taccccagcg aagccgccca caagtaactt gcggcggtga tttgtgtcag cagatatagc
     2341 cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc aatgaaaacc
     2401 agcaccagac tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct gcgatgaatg
     2461 aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc gaggccacgg
     2521 atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag gcgagcatga
     2581 gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac aatgccgcgg
     2641 catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca tgtcttgcac
     2701 gctggatatc gcgtatccgc tgtttttctg cgcgagccat cagataccag gcgataaatc
     2761 cgacaattcc gaccgcaccg agaattaacg ttgcaagggc gttgatttcc ggattcactc
     2821 ccatccgcac gctggaaaag accagcatcg gtaacgtggt ggctcccggc ccggaaacaa
     2881 agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag ccagaaatga
     2941 tcgcgggcat gatcatcggt agcgtaatga cgaaaaacac tttcagcggc gtcgcaccga
     3001 gatccatcgc tgcttcttct atcgagcgat ccagttcccg caatcttgaa gaaataacga
     3061 ccgccacata agccgtacaa aaagtgacat gcgccagcca gatggtgagc ataccgcggt
     3121 ccgcaggcca gccaatggca tgagcaagcg cgacgaataa taacaacagc gacaagcccg
     3181 tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaaacca tttgatccgc
     3241 gaaacctgcc aaaacgcacc agcaccaccg ccgcaatagt cccgaggatc gccgccgccg
     3301 ttgccgcaca ggcagcaatt gtcaggctta aaccaaccgc actcatcatc gcgtcatcgc
     3361 gcaataactc accataccag cgcgttgacc agccggccca caccgtcacc agcttcgagc
     3421 tgttaaacga atagataacc agcatcagca ttggcgcgta gagaaaggta aagcccagca
     3481 gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa attattcatc cgtgttctcc
     3541 cacgcttttt tgctggtgtt tgtggaacca cattatcggc acaattagca gcagcaacat
     3601 gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact cttgccataa
     3661 cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tccgggatca caaactcgcc
     3721 cacagccggg ataaacacca gcatcgatcc ggcaataatc ccacctttag tcagcggcac
     3781 gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg cttccaccag
     3841 cgaataatca atacgaatca acgcggtata aatcggcagt accataaacg gcacgtaagc
     3901 gtaaacaatg ccgatataaa cggccagatt ggtatgcaga atggtcagcg gttgatcgat
     3961 aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata ttcccatcca
     4021 ggcataaacg cggatcagaa acgaggtcca cgacggcagg atcaccagta gtaataaaat
     4081 attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc cgatcagtaa
     4141 acagcaaaat gtcgaaatcg ccgccacctg taacgactgg agataagcat caaaatagag
     4201 cggatcgtcg gtcagttgca gaaaattacc gagattaaga gtgatggaaa gttgcccgtc
     4261 agcccactcc atcagttcgg tataaggtgg aatagcgcgc gccatctctc ccaggcttat
     4321 tttaaagaca atcagaaatg gcagcagaaa cagcaagatc aaccagatat atagcaacgc
     4381 aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct taaatccgcc
     4441 cggcggtttc gactgggcag caggttcaag tgtactcatc tttgctcctt aaaccgtcag
     4501 caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg tcggtaaccc
     4561 tttacgatga cgatgggcgt tttgtagctg ggcgctgatc atctgcccac ttttcagtcg
     4621 aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc ccaccgcgaa
     4681 gttacaacca ttggcgggcg gctcttcgca aagcatgatt ttttccgggc gcagcgctac
     4741 gtgtaccggc acgttatcga ccactgaagc atccgcatcg actttcagtg gatgcaccag
     4801 ccccggcgaa tcaagcacca ggccatcttc ctgacgctct ttgagtacac cttcaaagac
     4861 attaaccgaa ccgataaatt cagcgctgta gcgggtagtt ggatgctcgt agatctcttc
     4921 cggctcgcca atctggacaa atttcccgcg attcataatg gcgatacgcc ccgccatggt
     4981 catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacgc gctccagaat
     5041 atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc ccatcggctc
     5101 atcgagcagt aatagtttcg ggcgtttggc aaggcttcgg gccagggcca cacgctgtcg
     5161 ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatgt gcaccaggcc
     5221 gagcatctca tggacccgac tggcaatttc cgctttcggt agtttgtcct gtttcaggcc
     5281 aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact gaaacatcat
     5341 attgatgggg cgcaggtaag gcggcacctg tgacaaatcg acgccatcga gcattatctg
     5401 cccggcagaa ggttgttcaa aacctgccag catacgcagc agcgtggact tgccacagcc
     5461 ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga catcatcgac
     5521 cgcatgctga ccatcgtagg atttggtgag gttgcggatt tctaatagcg gcgtcagcgc
     5581 cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc tccggcattg
     5641 aatgtgtgca agcgcaccat gatggtgcga attgccggct ggtgtggtgc gcccgcccgg
     5701 catctaaggc tgcggattat tttccgctct tcactttggt ccacgcgcgg gtgcgcacac
     5761 ggtcgatttt cggatcctgt actttcagag tgaatagctt cgcacgaacg tccgccggag
     5821 gataaatacc cgggttctca cggacttccg cactcaccag cggcgtggct gctttattgg
     5881 cgttggcata gaacacatgg tcggaaatat gcgctactac atccgggcgc agcaggtaat
     5941 tcaggaactg ataggcttcg tctttgtttt tggcatccgc aggcatggcg aatacatcaa
     6001 agaacgccat cgccccttct tttggaatcg agaacgagac attcactcca ttcttcgctt
     6061 ccttcgcgcg gtttgacgcc tgccagacat cacctgccca gccgatagcg acgcaaatat
     6121 cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg ttcgggcgca
     6181 gctttaacag cagatcggtt gccggtccgg tgtaatcatc cgctttagtg ctgttgggat
     6241 ctttgccgag ataattcaac acggtagcaa aaacttcttc tggcgcatcc aggaaagaga
     6301 caccgcagct tttcagtttt tccagatttt caggtttgag gatcaagtcc cagctatcga
     6361 cgggcgcgtt ttcgcccagc acagctttaa ctttatcaac gttatagcca atcccggtcg
     6421 tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg accagcttca
     6481 gcagctccgg gtcgagattc ttccactccg gcaatttgct tttgtccagc ggctggaaaa
     6541 ctcccgcagt caactggcgt tcaagaaagc tggcagatgg aaccaccaag tcaaagccgg
     6601 tgctacccgc cattaatttg ccttccagta cttcgttaga gtcgaaaaca tcgtagacga
     6661 ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata taatcagacc
     6721 agttataaat gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag acggccatca
     6781 gagcacccgc agccagaccc gaaagccatt ttttatttaa ggcggtcata tctctttcct
     6841 tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact aaaagatatg caataaacgt
     6901 gcatattttt tatcggcggg gtgtaagaaa agagaactct ctcccggcca gcactgctcg
     6961 ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag ctcggattaa
     7021 aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc caaatcgaca
     7081 caagagaagt gaaataatct ttaagaaaag gttaaaaaca gcagataatt ttgcagcgcg
     7141 cagccactgc accagcgact gcgtgctcga aaggagttat cagtggagga aatagttttc
     7201 ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg cattagcttc
     7261 aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa actgctcata
     7321 ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca gattatcatc
     7381 ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag ataaatccgc
     7441 cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca ggatcgtgtt
     7501 atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat ggggcagatg
     7561 cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcaa gccattgacg
     7621 cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc tctttcgata
     7681 aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt aatgtgattg
     7741 cgactaacca cccgttttca ggcaatattc tgtcgtagcg tggcgttcga tccagcggat
     7801 cattttaccc gcgatgtcga taccagtggt tttttctatt ccttccagcc ccggcgacgc
     7861 attcacttcc atcaccaacg gcccgcgatt agcacgcaga atatccacac cagcaacgtc
     7921 cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg tgatactggc
     7981 gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt tcgcccgccg
     8041 ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc acccttgcgc
     8101 ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag cgtcaatcac
     8161 gctttccgcc gcctgacgcg tctccgccag cacgacgcca attccctgcg tgccttcaac
     8221 caacttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg tatcatccgg
     8281 cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca gttgcatgga
     8341 acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat agctccccag
     8401 catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc caatacgcgg
     8461 gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag acgccgcagg
     8521 atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac cgcgctgtat
     8581 agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca atatggcaat
     8641 tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata catcgacgtg
     8701 gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa atgggcggct
     8761 ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat tattgctgcc
     8821 acgggtgagg taatattccg ccagttgctc gtcatactgc gccagtgcgt ctttatccag
     8881 cggttgatag ctgttttcat gcaccaaaat ggaggccggt aaacgcggct tgagatccgg
     8941 attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct gcggtaattt
     9001 cagcagtttc gtcaccgctt caatattatt gcgcaggccg ccgatatata ccccgcccaa
     9061 tcccagcgat tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat caacgacacc
     9121 gagcaacagt tgttccgcca ggccgagctg agcatccgga cagatctgta aatggcggtt
     9181 aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt gcccgccggt
     9241 cagcgtcacc agttcttcac gtaatgcttt gtcggtaatg cgaataatgc tactacactg
     9301 caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac actgcgcttc
     9361 ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa gttcaatggt
     9421 tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg ttcatcagct
     9481 gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga agcatgagca
     9541 aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc tcaggcagcg
     9601 acagggagtc gcttaccgac agcagcgcca ccgccagtag catcattccg ataaattcca
     9661 gtatcaacac gcctttaggc aatttaccga tcgcgcgcat acgcttccct ctgcaaagtg
     9721 agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt ttagcaatta
     9781 atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt acaggcataa
     9841 atctatgagg agagaaataa tgcaaaccgt tatttttggt cgttcgggtt gcccttactg
     9901 tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt ttcagtatca
     9961 gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa aggcaggtaa
    10021 acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg gctataccga
    10081 ttttgctgca tgggtgaaag aaaatctgga cgcctgatcg tctgacaagc cctcgcgttg
    10141 agggctttac tgattttttc tgtgctgtgg tttaaacaaa ctactgataa ataagaaaca
    10201 cagtgccccc agcgcacacc agaacaccgc gcttagtaac catgccagct cttgccagaa
    10261 tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatt gaacagggtg ccgccagcat
    10321 tgcgccaaat agaggtttca ggacttctct acgctgtgaa aagaaactgg caactgctcc
    10381 aggaagaatg aaaaacagca agccgatttc aggatgcccg gcagcccgaa aagcgccttt
    10441 catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg acaaaacagc agattgcccc
    10501 cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc gaacacattt
    10561 ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta atataggcca
    10621 acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata tattgttact
    10681 ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa aaaacattat
    10741 cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc tccgtgaata
    10801 taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg gtcctcgcgc
    10861 ttgggctatg tctcggaaag ttacgacttg gttcgatcca actgggtaat tccattggcg
    10921 ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc gatgcgctta
    10981 atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg aacttttttt
    11041 ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg gttggcagtg
    11101 cgctggtgat cgccttaggg ttaggtaagc tgtttggctg ggatattggc ctgacggccg
    11161 gtatgttagc aggctctatg acgtcgacac cggttctggt cggtgctggc gatacactgc
    11221 gtcattccgg catggaaagc aggcagctct cactggcact ggataatctg agcctcgggt
    11281 atgccttaac ctatttaatc ggtctggtga gtttgattgt tggtgcgcgt tacttgccga
    11341 aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa cgtggcctgg
    11401 acactgatgc caaccgtaag gtttatttac cggtgatccg cgcctatcgc gtcggcccgg
    11461 agctggtggc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat cgacaaaccg
    11521 gctgctacat tgaacgtatt cgacgtaacg ggattctggc aaatccagac ggtgatgccg
    11581 tgctacaaat gggcgatgaa atagcgttgg taggctatcc cgacgcccat gcccgactcg
    11641 atcccagctt ccgtaacggt aaagaagttt tcgatcgtga ccttctcgac atgcgtatcg
    11701 tcactgaaga agtggtcgtt aaaaaccata acgctgtagg taaacgtctc gcacaactga
    11761 agttgaccga tcacggttgc ttccttaacc gcgtcattcg tagccagatt gagatgccga
    11821 tagatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggt gatgcccgtc
    11881 gcgtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag gtcactgacc
    11941 tgctggcatt ttgcgccttc tttgttattg ggctgatgat cgggatgatc accttccagt
    12001 tcagcacatt cagtttcggc atggggaacg ctgccgggtt gttattcgcc ggaattatgc
    12061 tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcaaggt gcattaagca
    12121 tggtgaaaga gttcggcttg atggtgttta tggcaggcgt tggtctgagc gccggtagcg
    12181 gtattaataa cggcctgggc gcgattggcg gtcagatgtt gattgccgga ttaattgtca
    12241 gtcttgtgcc cgtggttatc tgtttcttgt tcggtgctta tgtattgcga atgaaccgcg
    12301 cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg gagatcatca
    12361 gtgatacagc tcgcagtaac atccctgcgc tgggctatgc gggcacctac gcaatcgcca
    12421 acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga ttaggataaa
    12481 actgaagttg ccctgaaaat gaaatttttt tgcacaaccg cagaactttt ccgcagggca
    12541 tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc attttgtgga gcccatcaac
    12601 cccgccattt cggttcaagg ttgatgggtt ttttgttatc taaaatttat ctactaccct
    12661 gcaaccctct caaccatcct caaaatctcc tcgcgcgata gcggcttacg gtcggtgaca
    12721 aaatgcagcg tcatcccctc aataaacgca tcaagcgcgc gggcggttcc gggttcaaac
    12781 cattgttcga gcgtttgctg actgcgctgc atccagtttt gcattaccgt ttttaatagc
    12841 ggttttcggc tagccagcgc gtagagctgg tacatcagct ccatattatc cggcgttgca
    12901 acctgtgagc tgtagatcat atcggtgata gcctggcatg cgccttgagc atcactaaca
    12961 tcgctaaaaa atgcctgata ttgccgggac ataatctcag taaaactgct gaacgcctcc
    13021 agcaacaact catcaattcc tgaaaaatag taggtcatcg atcccaacgg taccccggca
    13081 agggtagcaa ttttgcggtg cgtaacagca tgtattccgt aaagtttcac cgcctccagc
    13141 gtggcctgga taattttttc tcgccgttgc ggatcgttag cgcgacgcat gtcatttcct
    13201 ctgtctgcaa ttgtgtacaa atgtacacaa ccttgctaat gttgtcttcc ctcttcttat
    13261 tttgacgtga tctatgaccg taaattcttc acgtaatgca ttgaaacgcc gaacctgggc
    13321 gctgtttatg ttcttctttt tgccaggcct gttaatggcg tcctgggcaa cccgtacgcc
    13381 tgctatccgc gatattctct ctgtctcgat cgctgaaatg ggcggtgttc tctttggtct
    13441 gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg ttagtgaaac gctttgggac
    13501 acgtaatgtc atcctggtca cgatgtcctg cgcattgatc gggatgatga tattaagtct
    13561 ggcactctgg ctgacatcgc ccctgctctt tgccgttggt ctcggcgtct ttggggcaag
    13621 ttttggttct gcggaagtgg cgataaacgt tgaaggtgcc gccgttgagc gagaaatgaa
    13681 taaaacggtt ttgccgatga tgcacggttt ttatagccta ggcacgctgg caggcgctgg
    13741 tgtcgggatg gcactgacgg cctttggcgt tccggcaacg gtgcacattt tattggcggc
    13801 gctggtaggt atcgcgccta tttatatcgc cattcaggca atccctgacg gtacgggcaa
    13861 aaatgctgcc gatggcaccc agcatggcga aaaaggcatt cctttttatc gcgatatcca
    13921 gttgctgttg attggtgttg tagtgctggc gatggccttt gccgaaggtt ctgccaacga
    13981 ctggttaccc ttattaatgg ttgacggtca cggttttagc cctacttccg gctcgctgat
    14041 ttatgccggt tttaccctag ggatgaccgt tggacgcttc actggcggtt ggttcatcga
    14101 ccgttacagt cgcgttgccg tggttcgggc cagtgcacta atgggggcgt tgggtattgg
    14161 gctgattatt tttgtcgata gcgcctgggt cgctggggtg tctgttgtac tctggggact
    14221 gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc agcgataccg gccccgatgc
    14281 accaacccgc gtcagtgtgg tagcaacgac cggttatctg gctttcctcg tcgggccgcc
    14341 gctgctgggc tatctcggcg aacattatgg attacgtagt gcaatgctgg ttgtactggc
    14401 gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc aaacccgata ccaaaacgcg
    14461 gacggcgatg gagaatagtt gatgagcatt aaattaattg cggtagacat ggatggtact
    14521 ttcttaagcg atcaaaaaac ctataaccgt gagcggttta tggctcagta tcagcaaatg
    14581 aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc aatattatca gttgctctct
    14641 ttcttccctg aaattgctaa tgaaattgcc tttgtggctg aaaacggcgg ctgggtagtg
    14701 agtgaaggca aagatgtttt taatggcgag ctgtcgaagg atgcctttac tactgtcgtg
    14761 gaacatttgc tgacgcgccc ggaagtggaa attattgcct gcggaaaaaa tagcgcctat
    14821 acgctcaaaa aatatgacga tgccatgaaa acggtggcag aaatgtatta tcaccgtctg
    14881 gaatacgtcg ataactttga caacttagaa gatattttct ttaagtttgg cctgaatctt
    14941 tccgatgaac tgattccaca agtacaaaaa gcattacatg aggccatcgg cgatattatg
    15001 gtgccagtcc acaccggcaa cggcagcatc gatctgatta tccccggcgt acataaagcc
    15061 aatggccttc gccaactgca gaaattatgg ggaatagacg acagcgaagt ggtggtcttt
    15121 ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag gctttagttt tgcaatggaa
    15181 aatgccggca gcgcggtcgt cgcagcggca aaataccggg caggctccaa taaccgtgaa
    15241 ggcgtactgg atgtgatcga taaagtgctt aaacacgaag cgccatttga ccaataaaaa
    15301 cggcagcacg cccggcaaca caatacctga atgaaggctg cataattcgc ggaaattaac
    15361 ccgctgcatt atttcaggcg aaatgattat gaaaaattgt ctactgttgg gcgcgctttt
    15421 aatgggcttt actggtgtgg cgatggcgca aagtgtcacc ggcgatgtgc cgagtggata
    15481 caaagtggtt gtagttcctg attcagtcag cgttccacaa gcggtcagcg tcgcgactgt
    15541 gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc atccttatgc
    15601 tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg acgaccatca
    15661 ccattaattc gagaatgcct gatcgcacaa agcagtcagg cattttttta cccttcgtga
    15721 gaatttccca tctgtttatc ttttaaaaag ataaccatca gcgacagcca caaaattccg
    15781 ttgacaaggt tgaagagatt aaactgtccg ttgcccccgt tcagccaggc atgtttgctg
    15841 atttcaatac caacggtaaa gatcagcatt tgcagcattc ccatcgccgc agaaaccgta
    15901 cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt cgccagacca
    15961 ataccgaaag cataaatact taacccggca gtcatccata aatacgcgtg cgatgagata
    16021 accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc cataattatc
    16081 acgtacggtg cggcgcgagg tcagacgcgc taacagcaag ttacccgcaa ttaacgcccc
    16141 gaaaataggc acttgcagca agccatattc atagctgctc aactgctccc cggtaatgat
    16201 gataatcggc gactgggcga tccacgccag caatggcagg ctaacgaatc ccagcgccag
    16261 cgcccccgcc acaaagcggc cgttcttcag caccagctta tagtcacgac cgagttcttt
    16321 cagtgacagt ttctcgccta tacgcgtggc ggtttcaggc atagctcgtt gcagaccgaa
    16381 aaaggagatc gctgccaatg cggcaaacaa aacaaacatc ccctcccagg gcagcacatg
    16441 gatccacgcc gcgcccacca gcggaccaag tagcggagca atcagcgcca cgttcgccat
    16501 cagcgcggtg atcttgatac aaaccgcctc ttcgaaggat tcctgaattg cggcgtatcc
    16561 cacagcgcca atgaaacaga ggcttatgcc ctgcaagaag cgcaacaggg tgaattgttc
    16621 aatgttttgc gccagtaata ttgccagaca ggtgacgata aaccacacca cacccgccag
    16681 catcaccgga cggcgaccaa tacgatccga cagcggcccc aggagccatt gtaaaaacat
    16741 cccgcccgcc agatacgcgg tcatcgaagt aggaacccaa tcaatgcccg cctgatattg
    16801 ttccaccacg gccaacatac cgggttggat catatcgttg ccgatatagg ttgaaaattc
    16861 gtaaagcacc agacagagag ggaaaagtaa cgcctgacgt ccaagcctgg caccgaaagc
    16921 taatttattt tgcatgcaat ttcttcgcca ataataatcg cgcagagttt aataaaagcg
    16981 cagctaacga gaaagcgaat tttgtagctg aaaccacggt taagcacatt cttacattat
    17041 tgcgagtata gctacgcttt ctttaagttt tatttaacct atgcccgtta caatcaccca
    17101 ccgtaaacag gccgcttgag ggaaataaga cgatgccgct ttacccagtt taacctgcac
    17161 tttattctca acgacttgcc tgtattggct cccttttaat cactttgcgt cgggaagtta
    17221 tgctggaaaa tttgaatctc tctctattct ctcttattaa cgcgacgcca gactcggctc
    17281 cgtggatgat ctcgttggcg atttttattg ctaaagattt gattaccgtg gtgccgttgc
    17341 tggccgcggt actttggttg tgggggctta cagcacaacg gcaactggtg ataaaaatcg
    17401 ctatcgcgct ggcggtcagc ctgtttgtgt cctggacgat gggacatctt tttccgcacg
    17461 accgaccctt tgtcgaaaat atcggctata acttcctgca tcatgcggcg gatgactcat
    17521 tcccgagcga tcacggtacg gtgattttca cctttgcact ggcattttta tgctggcatc
    17581 gcctgtggtc cggctcactt ttaatggttc tggccgtcgt cattgcctgg tcgcgcgttt
    17641 atcttggcgt ccactggccg ctggatatgc tcggtggatt gctggcaggt atgattggct
    17701 gccttagtgc ccagattatc tggcaagcga tggggcataa actctatcaa cgtctgcaat
    17761 cgtggtatcg cgtctgtttt gcattaccga tccgcaaagg ctgggtgcgt gactgatctc
    17821 tgctaaaaag tgtagtattg agcggctcgc ttcaataact attcagaggg attatggaaa
    17881 cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt aaaacgcagc gataagttac
    17941 atcttaaaga cgccgccgcc ctgcttgggg tttcggagat gacgattcgt cgcgatctga
    18001 acaaccacag tgcgcccgtc gttttgctcg gcggctatat tgttctggaa ccgcgcagtg
    18061 ccagccatta cctgttaagc gatcaaaaat cccgcctggt ggaagaaaaa cgccgggcgg
    18121 caaaactggc tgcgacactg gtagaacccg atcagaccct cttttttgac tgtggcacca
    18181 ccacgccgtg gattattgaa gcgattgata atgaaatccc ttttaccgcc gtttgttatt
    18241 cgctaaatac ctttctggcg ctgaaagaga aaccccattg ccgcgcattt ctgtgcggtg
    18301 gtgaatttca cgccagcaac gccattttca accccatcga ttttcagcaa acgttgaata
    18361 atttttgccc ggatatcgct ttttattccg cggcgggcgt gcatgtcagt aaaggcgcta
    18421 cctgttttaa tcttgaagag ttgccggtaa aacactgggc catgtcgatg gcgcaaaagc
    18481 atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg tccggcgcgc atgggtgacc
    18541 tgaaacgctt tgatattgtg gtgagtgatt gttgcccgga agatgagtat gtgaagtacg
    18601 cgcagacgca ccgtattaag ttgatgtatt aatgacgtat aaccggatga cgtttcgcgc
    18661 catccggtta tcagaagatt aagagaacca gctgccgaac cactgatgga atttcatcat
    18721 cacgaaatcc cacatccgac caaagaatcc gccctcttcc acattttcca tcacgatcag
    18781 cggacgctgc tcaatggatt taccgttaag ctggaaatca atggtcccga caacctgacc
    18841 ttttttcagc ggtgcggtaa gctgcggttc cgttaacgtg tagctcgcct tcaggttttt
    18901 cagctgccca cgcggtatgg tcacggagcc cgcttcgcct gccccgagat tcacttcgct
    18961 cttatcacca aaccagacgc gctgagtcac aaaggtggca tcaggtttaa ttggcgtcac
    19021 agtttcaaag aagcggaaac cccaggtcaa taatttctca gactcattaa aacggatacg
    19081 gtcagttttc gcccccagca ctacggagat taaacgcata tcaccctggg tagccgaagc
    19141 aaccagatta tatcccgcac ctgccgtggt tcctgtcttc atgccatcaa cattcagatt
    19201 gctgctccac agcagacggt tacggttagg ctgacgaatt ttgttgaagg tgaactcttt
    19261 ctctttatga atggcgtact cttccggcac atcgtggatc aatgctttac ccagcaatgc
    19321 catatcgcgc gcggtactga actgccccgg cgcatccaga ccgtgcaccg tctggaaggt
    19381 agtgttggtc agacccagtt ttttggcata accattcatc aaaccaataa atgactcctg
    19441 gctcccggca acgtaatcgg ccagcgcaat acaggcgtca ttaccggact ggataatcac
    19501 acctttgttc aagtctgcca ccgaaacctg atcgcccggt ttgaggaaca ttaccgatga
    19561 accacgcagt gccggatttc ccgtcgccca ggcatcttta ccgaccgtca ccatatcggt
    19621 gagtttaatc ttatcggcct taagcgcctg cccaaccaca tagctggtca tgattttagt
    19681 caggctcgcg ggatccagtt tctcatccgc gttgccttct gccagcactt taccgctggc
    19741 gtaatccatt aaaatccatg cacgcgcatc cacgctcggc gcttcaacgg tttgttccgc
    19801 cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa cccgctgcaa gaccacgaag
    19861 gagagaggag tattgcgtca taaatgccac ccgagtatcc attcaaaaaa aatacgttat
    19921 atcgccgtat gataagttcg gtgattaata acgcacacac aaacttaaag aaaccgtaag
    19981 ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt cgctgcttct ggtgtcattt
    20041 ttttgccagc tgttgcttaa aagttaactt tacttcacca tcggagtcct gttcagcctg
    20101 tggctgcctg tcggaaaagg ggtgagtatg attacgctgt ggggtcggaa taattcaact
    20161 aacgtaaaaa agtattgctg acgctcgaag aactggaact accttatgag caaattctcg
    20221 cgggccgtga gtttgggata aatcacgatg ctgattttct ggcgatgaac cctaacgggc
    20281 tggtgccgtt gttgcgtgac gacgaaagtg atctcattct ttgggaatca aacgccattg
    20341 tccgctatct ggcggcacag tacgggcaaa aacgcctgtg gatcgactca ccggcacgtc
    20401 gtgcggaagc ggaaaaatgg atggactggg caaaccagac gctcagtaat gctcatcgcg
    20461 ggatcctgat gggattagtc agaacaccac cggaagagcg cgatcaggcc gccattgatg
    20521 ccagttgcaa agagtgcgac gccctgtttg ccctgctcga tgcggaactg gcaaaagtaa
    20581 aatggttctc tggcgacgag tttggtgtgg gcgatatcgc tatcgcaccg ttcatctaca
    20641 atttgtttaa cgttggcctg acctggacac cgcgtccaaa tctgcaacgc tggtatcagc
    20701 aactcactga acgccccgca gtgcgcaaag tggtgatgat tcccgttagc taattgcgtg
    20761 ggctaacttt aagtaattcc ccactggact cgtcggtgag aacgtataaa taaccgtcgg
    20821 gtccagtgcg aacatcacga attcgctgcc ctctgtccgt taaaatacgg ccatcttctg
    20881 tcactttgtc gccgttgacg ctcatcacaa tgacatcttt atctttcagc gcgccaataa
    20941 ataatttttg ctgccactgg gggaatttat cgctgttata gaaggccatg ccgctcacag
    21001 cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc ggcgacgatc tcccctttcg
    21061 cttccggtat cttaaagcct gaatagttga ttccccaggt tgccagcggc cagccgtagt
    21121 ttttgccttt ttgcgggata ttaatttcat caccaccgcg cgggccatgt tcattcagcc
    21181 acagtgcatt actccacgga ttcatcgcca ttccttgcgg attacgaatg ccataagacc
    21241 agatctcggc gcgcgcaccg gattccttta taaaaggatt atcatccggg atttcgccct
    21301 ggtcggtcag acgcaccagt ttgccctgta atttatccag atcctgcgcc gtcgggcgct
    21361 gattgttttc gcccagagca ataaaaagat aacctttacc gtcgaatacc agccgcccgc
    21421 caaaatggtt gccggtagac agttttggca tctggcggaa gacggtgcgg aagtcggtca
    21481 cttttgagag atcatcactt aagcggccat aacccacagc agttccggct ttgccatcat
    21541 cgccaacttc ggaataactt aaccagatgc ggcgagactg agcaaaatca ggcgctaaaa
    21601 ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc cggaactccg gaaagcggcg
    21661 cagataatcc ttttcctgct tgccagtgac gcaactcgcc gccgcgcaga gtgattaaca
    21721 taccgtgatt atcgggtaaa aaggccagtg cccagggatg gtcgagtttg tcttgcagta
    21781 cttcgacatt taccgttgca ggagccgccc agagagcgga agaaagacaa ataaggggca
    21841 caaggaaaaa ggattgtcga tgcataacgc ctccggattg actctggctt aaagcgtagt
    21901 cagtggagga gataacaaat tcatttttac aaaaacttaa acatgaaggg ggagacgctt
    21961 tctccccctt agttttcagg ccttctcaag catggcgtgc ttctgcaggc tctggatact
    22021 cagcgttaag ctcatcagac aattttcaag cttatcggcg ttgacggtaa taacagtcgg
    22081 gcaatcatgg tgcccactca tcaaacatac tgcggctgtc gctaatgctt cttcagcatg
    22141 atgaagagca ctccactctt cctgatccag atgaagattc aaccgcagcg atttatcgtg
    22201 cagttcgcga ttcagtttaa aaaagttatc tcgtagatga ttgctttcgc tgacggacat
    22261 gtatcctttt gccttcctca gttgcacgta tgcagcgagg tcaacgcgtg cgtcgatcaa
    22321 ccggttcagc agatcgattc gctgtctgtc aacgaacata cttcgatcct cctctttcca
    22381 gccttgttgc gattaaatag tatggctatt ttttgagcaa aatgctgtcc aggatcaatt
    22441 cttcacgcca aattaacaaa atttacattt ccttcgcctc cgccagcgaa tgagattgcg
    22501 atacaaattt tatccagaac tctgtagaat ccctgccctg acctttcctg taactgaata
    22561 ttttttaagc tatgagcaaa gtaactcccc agccgaaaat cggctttgtt tcccttggct
    22621 gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga actacgcact gaaggttatg
    22681 acgtggtacc gagctatgac gatgcggaca tggtgatcgt caacacctgc ggctttattg
    22741 acagcgcggt acaagaatca ctggaagcca ttggtgaagc gttgaatgaa aacggcaagg
    22801 taattgtgac cggttgtctg ggggcaaaag aagatcagat ccgcgaagtc cacccgaaag
    22861 tgctggaaat caccgggcct catagctatg agcaggttct ggagcacgtt catcactacg
    22921 tgccaaaacc gaaacacaac ccattcctga gcctggtgcc agaacaaggt gtgaaactga
    22981 caccgcgtca ttatgcctat ctgaaaattt cggaaggctg taatcaccgc tgcaccttct
    23041 gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc gattggcgaa gtgttaagtg
    23101 aagcgaaacg tctggtagat gcgggcgtta aagagattct ggtgatctcg caggatactt
    23161 ccgcctatgg cgttgatgtt aaacatcgta ctggcttcca caacggcgag ccggtaaaaa
    23221 ccagcatggt cagcctgtgc gaacagttat cgaaactggg gatctggaca cgtctgcact
    23281 acgtttaccc ttatccgcat gtggacgacg tcatcccact gatggcagaa ggcaaaatcc
    23341 tgccgtatct ggacattccg ttgcagcacg ccagcccgcg cattctcaaa ctgatgaagc
    23401 gtccgggttc tgtagatcgc caactggcgc gcatcaaaca gtggcgcgaa atctgcccgg
    23461 aactgaccct acgctcaacc tttattgtcg gcttccctag cgagacggaa gaagatttcc
    23521 agatgctact cgacttcctg aaagaagcgc gtctggatcg cgttggctgc tttaaataca
    23581 gcccggttga aggtgcagac gccaatgccc tgcctgacca ggttccggaa gaagtgaaag
    23641 aagaacgctg gaaccgtttc atgcagctgc agcagcagat ttccgccgag cgcctgcaag
    23701 agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt ggacgaagaa ggcgcgattg
    23761 gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt ttatctcaat ggtgaaacca
    23821 acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca cgccgatgag tacgatttgt
    23881 ggggtagccg ggtttaatta attcaggccg ggcaaaatca tttgtccgcc ctgcgaataa
    23941 tgattactgc cagctaatct gagccctgcc gtttttctta ctcttataca gccgctcatc
    24001 cgccagcatt ttcaagcgtt cgacggtggt acattcattt gaatgcgcta caccgatact
    24061 ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt tcttttagaa tcgatagcaa
    24121 ctcttccagc gttccacccg tataaatcac cgcaaattcg tcaccgccga agcggtagat
    24181 tttaccttta tctaccggga tctgtgattt aaggattttc accactttca tcaacacttc
    24241 atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga cgtcaaacag
    24301 gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg attttctgcc
    24361 cattgctttg gtcaggtcgt ccaggaacgc cgagttatac atactgtaga gttttttgac
    24421 atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt tattaatgat
    24481 ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga ggcaactggt
    24541 acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacaga cccgcaaata
    24601 aacatctttt ttatacgcaa atacgtacaa tccggtcggc aaataggcga agtgctccat
    24661 cgcttccgaa acgatgaaga tggcgttttt agcattatat tcatcaccgg tttaccgttc
    24721 ttaaatagag agaacatcag gttctcgctg tccatcgcca gcgattttga cagtaaagaa
    24781 aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa
    24841 tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg tcgatacttc
    24901 acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg
    24961 tcactggaaa tcagataagg accgacatcg tacaaataag agggggaaac cgagtcggcg
    25021 taagctgtgc gcaaaccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga
    25081 tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc agtatcaata
    25141 acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat cccgcaggca
    25201 actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt attgattctg
    25261 gacatacgta aactactctt ttactaattt tccactttta tcccaggcgg agaatggcat
    25321 gggtttctgg tacagatacc cctgagcctg aacacatcct aatttaatta attcgcgcgc
    25381 aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc gtgccagatc
    25441 gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa attgcgagtc
    25501 cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca aaccggtgcc
    25561 aaagtcatca atcgatatcc gtaccccttg tttacgcagt tgttcaatat tgagataaaa
    25621 atgttcatta attcgctcga tcacggtttc agtgatctca acacagaggc tgttcgcctt
    25681 caggccattt tctttcatga tggtggtaaa acgctcatga aagtgcggtt cgtttaactg
    25741 ccagggcgaa acattaatat gcagtaagaa atcatccttc agcgacgcac gctgactaaa
    25801 atgtctgaat tcacccattg ccgttttaat aatctgataa cccagctcat tgataaaccc
    25861 gatatcttca gcaatggtaa tgaacttcag cggtgaaatg atcccccgct ccgcagaaac
    25921 ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa cgatcggttg
    25981 atagacgagg aatagttcgc caatttccat tgcatggcga atattacgcg caatccagat
    26041 attctcttct atttcgttga gtttcgcgtc atcacagaaa ataaactcgg agaacatatg
    26101 gtcacggata ctggaaatcg ccagtttcag tttttctgcg taactccagg cgttttcccc
    26161 ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga ttgcggtagc caaagctgct
    26221 gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc tgtacatttt cccgacacag
    26281 aagtaaatag agatcggcat tgaatgtcac gatatcgcag cattgcgcat atttctcggc
    26341 aattcgctga cacaatttcc tgacgatttt attgcgttca tcaacaccaa acaccgcttc
    26401 cacggcttcc agaccacaca gtttgatgat ccccacagcc agatactgat tattgttgga
    26461 ctcaataatc gccatatttt taataaaacc gctttcctga tcgtaagaga tcagtgagag
    26521 caatttattt gaccgttcaa tttcggcatc tttcatttcc gcgatcg
//
LOCUS       DAZPLM010000036        26512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPLM010000036 DAZPLM010000000
VERSION     DAZPLM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.036"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHVC7_001287"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHVC7_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR2115388.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHVC7_001288"
     CDS             complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHVC7_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR2115389.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHVC7_001289"
     CDS             complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHVC7_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR2115390.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHVC7_001290"
     CDS             complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHVC7_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR2115391.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHVC7_001291"
     CDS             complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHVC7_001291"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR2115392.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHVC7_001292"
     CDS             complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHVC7_001292"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR2115393.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHVC7_001293"
     CDS             complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHVC7_001293"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR2115394.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5321..6043)
                     /locus_tag="ACHVC7_001294"
     CDS             complement(5321..6043)
                     /locus_tag="ACHVC7_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2115395.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHVC7_001295"
     CDS             complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHVC7_001295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2115396.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            7243..7461
                     /locus_tag="ACHVC7_001296"
     CDS             7243..7461
                     /locus_tag="ACHVC7_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR2115397.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHVC7_001297"
     CDS             complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHVC7_001297"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR2115398.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(8195..8395)
                     /locus_tag="ACHVC7_001298"
     CDS             complement(8195..8395)
                     /locus_tag="ACHVC7_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR2115399.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8405..10210)
                     /gene="kefB"
                     /locus_tag="ACHVC7_001299"
     CDS             complement(8405..10210)
                     /gene="kefB"
                     /locus_tag="ACHVC7_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR2115400.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(10210..10761)
                     /gene="kefG"
                     /locus_tag="ACHVC7_001300"
     CDS             complement(10210..10761)
                     /gene="kefG"
                     /locus_tag="ACHVC7_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR2115401.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10892..12805
                     /locus_tag="ACHVC7_001301"
     CDS             10892..12805
                     /locus_tag="ACHVC7_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2115402.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12805..13827
                     /locus_tag="ACHVC7_001302"
     CDS             12805..13827
                     /locus_tag="ACHVC7_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR2115403.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13821..14039
                     /locus_tag="ACHVC7_001303"
     CDS             13821..14039
                     /locus_tag="ACHVC7_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR2115404.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            14093..14962
                     /locus_tag="ACHVC7_001304"
     CDS             14093..14962
                     /locus_tag="ACHVC7_001304"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR2115405.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(15017..15421)
                     /locus_tag="ACHVC7_001305"
     CDS             complement(15017..15421)
                     /locus_tag="ACHVC7_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR2115406.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15723..16355
                     /gene="crp"
                     /locus_tag="ACHVC7_001306"
     CDS             15723..16355
                     /gene="crp"
                     /locus_tag="ACHVC7_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR2115407.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            16406..18496
                     /locus_tag="ACHVC7_001307"
     CDS             16406..18496
                     /locus_tag="ACHVC7_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR2115408.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18563..19783)
                     /gene="argD"
                     /locus_tag="ACHVC7_001308"
                     /gene_synonym="dapC"
     CDS             complement(18563..19783)
                     /gene="argD"
                     /locus_tag="ACHVC7_001308"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR2115409.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19869..20432)
                     /gene="pabA"
                     /locus_tag="ACHVC7_001309"
     CDS             complement(19869..20432)
                     /gene="pabA"
                     /locus_tag="ACHVC7_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR2115410.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20464..21066)
                     /locus_tag="ACHVC7_001310"
     CDS             complement(20464..21066)
                     /locus_tag="ACHVC7_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR2115411.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(21056..21223)
                     /locus_tag="ACHVC7_001311"
     CDS             complement(21056..21223)
                     /locus_tag="ACHVC7_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR2115412.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21328..21900)
                     /gene="ppiA"
                     /locus_tag="ACHVC7_001312"
                     /gene_synonym="rotA"
     CDS             complement(21328..21900)
                     /gene="ppiA"
                     /locus_tag="ACHVC7_001312"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR2115413.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            22171..23352
                     /gene="tsgA"
                     /locus_tag="ACHVC7_001313"
     CDS             22171..23352
                     /gene="tsgA"
                     /locus_tag="ACHVC7_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR2115414.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23614..26157
                     /gene="nirB"
                     /locus_tag="ACHVC7_001314"
     CDS             23614..26157
                     /gene="nirB"
                     /locus_tag="ACHVC7_001314"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR2115415.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            26154..26480
                     /gene="nirD"
                     /locus_tag="ACHVC7_001315"
     CDS             26154..26480
                     /gene="nirD"
                     /locus_tag="ACHVC7_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR2115416.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6455 a         7190 c         6637 g         6230 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatgtaag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtcg tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcatattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcacg agcagcaccg ccgtaggttt tagccagtac ggtggtgatt
      841 gcagcggtca gagtagtttt accgtggtca acgtggccga tagtaccaac gttaacgtgc
      901 ggttttgtac gttcaaattt ttctttagac acggctatat tccttactat agtgctctcc
      961 ccttcaggag agagcacggg actttggtat taacccttag gcttatttac cacgggcttc
     1021 aattacggcc tgagcaacgt tactcggcgc ttcatcatac ttcaggaatt ccatagtgta
     1081 tgatgcacga cctttggtca gagaacgcag ctgagttgcg tatccgaaca tttcagacag
     1141 cggtacttca gcgtggatct taacgccagt aacttcagat tcctgacctt tgagcatacc
     1201 acgacgacgg ctcaagtcac cgataacgtc accggtgttc tcttccggag tttctacttc
     1261 aaccttcatg atcggctcaa gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc
     1321 gatagaagca gccagtttaa acgccagttc agaggagtca acgtcatggt aagaaccgaa
     1381 gtgcagacga atacccatgt ctactaccgg gtagcctgcc agcggacctg ctttcagctg
     1441 ttcctggata cctttatcaa cggccgggat gtattcgcca gggattacac cacctttaat
     1501 gtcgttgatg aactcgtagc ctttcgggtt tgaacccggc tccagcgggt acatgtcgat
     1561 aacaacatga ccatactgac cacgaccacc agactgtttc gcgtgtttac cttcaacatc
     1621 ggtaactttc tggcggatag tttcacggta agcaacctgc ggtttaccta cgttcgcttc
     1681 aacgttgaat tcacgcttca tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc
     1741 cgcgatgatg gtctggttag attcttcgtc agtccataca cggaaagacg ggtcttcttt
     1801 agccagacgg cccagagcca gacccatttt ttcctggtca gctttggttt tcggttcaac
     1861 tgcgatggag attaccggct cagggaattc catacgttcc agaatgatcg gcgcatccgg
     1921 gtcacacagg gtgtcaccag tggttacgtc tttcagaccg atagcagcag cgatgtcgcc
     1981 cgcgcgaact tctttgatct cttcacgttt gttagcgtgc atctgaacga tacgaccgaa
     2041 acgctcacgt gcagctttca cggagttcag tacggtatca ccagagttaa ccacaccgga
     2101 gtaaacacgg aagaaggtca ggttaccaac aaacgggtcg gtagcgattt tgaacgccag
     2161 tgcagagaac ggctcgtcat cacttgcgtg acgctcagcc ggagtgtctt taccgtcgtc
     2221 caggataccg ttgatcgcag gtacgtcaac cggggatggc aggtaatcaa ttaccgcatc
     2281 cagcatcgcc tgaacacctt tgttcttgaa cgcagaacca caggttacca ggatgatttc
     2341 gttgttcaga acgcgctgac gcagagcacc tttgatttct gcttcagtca gttcttcacc
     2401 acccaggtat ttttccatca gctcttcaga agcttcagct gcggattcga tcaggttctg
     2461 gtgccattcg ttagccagtt caaccatgtc tgccgggata tcttcgtatt cgaaggttac
     2521 gccctggtca gcgtcgttcc agttgatagc tttcattttc accaggtcaa caacaccggt
     2581 gaaatgttct tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt
     2641 tttgatctgg ttaacaactt tcaggaagtt cgcacccatg cggtccattt tgttaacgaa
     2701 cgcaatgcgc ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg
     2761 aacaccacca actgcgcagt aaaccattac cgcaccatcg agaacacgca tggaacgttc
     2821 tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc
     2881 atactgctta gccataccag accagaatgc agtagtagca gcggaagtga tggtaatacc
     2941 acgttcctgc tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat
     3001 tttatggttt acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc
     3061 gatgtgcgca ctgataccga tgttacggta gcgtgcgatg ggtgttgtac gagccatttg
     3121 tttcctcgtt tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga
     3181 agcgcccgcc tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt
     3241 gttggcttcg gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc
     3301 tgcagcatca gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg
     3361 agcagcttca acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac
     3421 tggtacctga taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac
     3481 gttttcgaga gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt
     3541 ctccagcgcg ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat
     3601 atttacaaat ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg
     3661 accaatgacg cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa
     3721 ctctacgagt ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca
     3781 ttaagcctta ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc
     3841 ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg
     3901 accgccacgg atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta
     3961 ggaagtcact tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt
     4021 cggtttttta ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc
     4081 ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg
     4141 gttaactgtt gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa
     4201 aacgtcctca cacaatatga ggacgccgaa ttttagggcg atgccgaaaa gatgtcaaga
     4261 aatatacaac gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg
     4321 acgaaatcag tatagtcaat gggaatgatg tcgttcgaaa tttgaccagt caaaccgcgg
     4381 gcaataaggt cttcgttcag ggcatagacc ttaatggggg cattacgcag actttcaagg
     4441 tagcggttac cgtcaactgc ggcagttacg ccatcttgca ataatagcag ttcgtcgcct
     4501 tcactgagca gacgcagcag cgcagcaaaa tccgccagcc agggtgaacg atgtaatgtg
     4561 tgcagcataa aagcctcaaa acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa
     4621 ggcatctgct tcgagcggcg tggcttcgac aacaaagggg gtctgcggat ctaacccgcg
     4681 ttcgcgcagt gaagccgcac aaacccagca ctgttcaatg tcgtacagac ccaacaattt
     4741 aaaagtggca atgtaatcac gcgccagcac tgcatcgggc ttttgtcctg gcagcagctg
     4801 aaaaacgcca tcagctataa agaagacagc cagatcatca gttaatgcgg aagttgccag
     4861 taaagcatct aaaccttccc gaccagcagc tgtaccatga ggcgcagtag aaaaaacaaa
     4921 cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc
     4981 gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg acgaggccag ccccagcctt
     5041 ccggcctccg tttcatcaac aacgccacgg cgtaatgctg ccgctacgca gatattcagc
     5101 gccacaccat gttgcgcatt caactgttgc cagccgcgca caaggtcaaa ttcgtcgctt
     5161 gccggtgagg tcaattggtt agcgttatag accccttccc gataaaaaaa gacgctgctt
     5221 aactcatggc cttctactat cagcgcctgc gcaaactgaa aagcactact cgcctgttgc
     5281 gtaccgtatg ctggcccggt caccacgatg gcaaaacgca ttacttatct tgcccctgga
     5341 aatcaccgct tttgaactgg cggatgtaga gatagacagt gtgtttggag atgttcaggc
     5401 ggtcagcaac ctggttgatc gcgtctttaa tatcgaagat ccctttctcg tagagattca
     5461 gcacgatctg acgatttttg gcgttattag aaacattgcg atcggcattt acttcttcga
     5521 tggtgaactc cagcgtttgg gtaaccagat cttcaacaga agaggcaaag ttgacgcttg
     5581 aaccgacatc cggggtttct ggcggcacaa aagtgctcat aatctgcgag aagggaacat
     5641 caagattcat attgatgcac agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga
     5701 tagtcaggga cttcattaat acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc
     5761 tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc caggtcagta attggcgaac
     5821 caatcttccg gcctgtatgt tcaccgttag caatgcgaat ggctgaacat tttagatcct
     5881 gcaaagagtg caaaacgatt tcacagtggg agccaataag catcgctaac ccgtccacca
     5941 ccgcttcgta ggatttcaga atatcaaaat cggtctggtc gaaaggacgt tgatccagta
     6001 aatccaactc actggtttcg ttggttaaaa gcgacctgga catgaaaaaa aacactcctt
     6061 ttcaggagcc tgtcgttaac ttttcagggc aggctcatta atgatgcggg taactaaatt
     6121 aatacagcgg aggttccgct ttccagcact aattatatcc ggcctgtaat aaaaaaaccg
     6181 ccgcctggtc aggcggcggt tcttaatgct tattttttag cagaatctgc ggctttcgca
     6241 tcagcttccg gctttgcatc agccttcggc gctggtttca catccagcag ctctacgtca
     6301 aacaccaggg tagaattcgg tgggatcccc ggaacacccg ctttgccgta agccagttct
     6361 ggtggaataa ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccac
     6421 cccgggataa caccgtccag acggaaagag agcggttcac cacgggtgta agagttgtcg
     6481 aactctttac cgtcgatcag cgtacctttg tagttcacta caacagtatc gctgtctttc
     6541 ggtgcttcgc ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc
     6601 acacctttct ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc
     6661 gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga
     6721 gtctgttcga tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca
     6781 gcgatcagct gatctttatc cagtttgatg cccagttttt cttgttcttt aagagagttt
     6841 tccatgtaac gacccagcga agcacccagt gcataagctg atttctgatc gtcatttttg
     6901 aacgctgctt tgctgtcagc agttgtagca ggttttgcag cttcagcagc aaaagtgatt
     6961 ggtgcatgca gggcaacggc cattgtggtc gccagcagcg ttactttaaa cagtgatttc
     7021 atccatatct ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact
     7081 ataaagcgtt gtcgaacaaa tctacataca gacacgccct attatcatct attttcagac
     7141 tctttttgtt taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt
     7201 taagtagaat ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg
     7261 gaagcacgcc tggcagagct ggagagccga ctggcttttc aggaaatcac cattgaagaa
     7321 ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt
     7381 ctgctgaccg agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa
     7441 acgccaccgc cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcacc
     7501 ttttcggtat tagtggcaac cacaaccgcc gttgccttta ccgccacagc agccttcgcc
     7561 accgtgttca tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc
     7621 gtggtgatga tcgtgcgcgc cgtgaacgtg accatgagcc agttcttctt cggtcgcttc
     7681 acgaatcgcc acaacctcaa cgttgaattt caggttctga ccggccagca tgtggttacc
     7741 atcaaccact acgtgatcgt cttcaaccgc agtgatttca accggtaccg gaccctggtc
     7801 ggtttcagcc aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt
     7861 aggaacacgt tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc
     7921 gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag
     7981 ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac
     8041 accgtcttct gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat
     8101 gatatctcct gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg
     8161 tactcactag cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca
     8221 cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc
     8281 cgcacttaac gcattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct
     8341 gtgcctgaca ggccgggcat tttgcgcccg caataaaacg ttttcggatt gccatcttta
     8401 ccctctactc aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct
     8461 ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acagtatcgg
     8521 catgcattgg gatgagctct cgcagcatcc gcatatccag gcggcgaaaa tgcagttgcg
     8581 cccgctgcgc ctgatgcgga tgcatcccaa gcgtgaccaa agtcttgcgc cccagctcta
     8641 acgcactgga gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg
     8701 cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata
     8761 tttccaccag cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg
     8821 cctctgcacc cgcagaacgt aatagatcga cctgcgtggc gtcgccgtaa taaactttgt
     8881 agccatattt acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca
     8941 ttttatttgc catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa
     9001 tgacctgggg tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa
     9061 actggcggga tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa
     9121 gcgtcaccgt caccagcaac agcgccatct ggtcgccctg gaataaacgt tgtgaagaag
     9181 cggtagaaaa gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca
     9241 tccgctctga gctacgcacg ccatacaatc gcgccagcag atacagcacg agaattttca
     9301 ccgccaccag cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga
     9361 ggttgagcga catgccgaca gagataaaga acaaaccgag cagcaagcct ttgaagggat
     9421 cgatagccgt ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa
     9481 atgtaccgag cgccatcgac agccccagcg catccataaa caatgcggaa cccaacacca
     9541 gcagcagcgt cgcggcggtg aacacttccc gcacgccaga agctgcaata aagcggaata
     9601 ccggacgcag taaatagcgc ccaccaatca gcatgccgac aaacgccagc accttcatgc
     9661 cgatcttcat ccagtcgaaa tgttcgtctg ccgaccccgc caacaacggc actaacgcca
     9721 gtgctgggat cactgccaga tcctgaaaca gcagaaccga aaacccgagc tggccggatt
     9781 cgctgcgatt catccctttc tcacgcatca attgcaacgc cattgcagtt gaagacatcg
     9841 caaggccaat gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc
     9901 ccgccagcaa cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgaacgcc
     9961 gcagttgcca aagtttggag ggattcaact caaggccgat gataaacatc aggaatacca
    10021 cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccacg
    10081 ggccaattgc aatccctgcc agcaaatatc ccaacacagc gccaatcccc agccgcgatg
    10141 ccaacggcac cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg
    10201 aaccttccat cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct
    10261 ggcgtggctc gccagctcct ttgcgctttg ccgtctcgcc cagtaaataa tgatgggact
    10321 taaccaatgc atccggcaca tgcccgccgc cagttcaaag gggcgcagca catcgctcat
    10381 cgggtagcga ttcagcgcgt cataacggta agcactttcc ggctcgccgg tggtaatcac
    10441 gttacgccag tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacgact
    10501 taatacccgg tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg
    10561 ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa
    10621 aaaatcggga tagtgcgcgt aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg
    10681 tttaagcagt acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa
    10741 aactttcgct ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt
    10801 tttgggctac cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat
    10861 aagttaacat agtctgaaca tacggcgcct tatgattgtt ttctcctcgt tacaaattcg
    10921 tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc atcaaccccg ggcagaaagt
    10981 cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga
    11041 aatcagcgcc gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa
    11101 tcaggaaacg ccagcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga
    11161 atatcgtcaa ctggaagcgc agctgcacga cgccaacgaa cgtaacgacg ggcacgccat
    11221 tgcgaccatt catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc
    11281 cagcctgctg cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt
    11341 ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt
    11401 gctgctgctc gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa
    11461 atggttgaag agctatcagg gcacgctgat cctgatctct cacgaccgcg acttcctcga
    11521 tccgatcgtt gataaaatta ttcatatcga acaacaaagc atgttcgagt acaccggcaa
    11581 ctacagttcg tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtatga
    11641 aagccagcag gaacgcgtgg cacatctgca aagttatatc gaccgtttcc gtgccaaagc
    11701 caccaaagcg aagcaggccc agagccgcat taagatgctt gagcgtatgg agctgattgc
    11761 ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa
    11821 tccgttactg aagatggaaa aagtcagcgc gggctatggc gatcgcatta ttctcgactc
    11881 gattaaactg aacctggtgc ccggctcgcg cattggtctg ttaggccgca acggcgcggg
    11941 taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg
    12001 tctggcgaaa gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc
    12061 cgacgaatca cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact
    12121 gcgtgactac ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg
    12181 cttctccggt ggggaaaaag cccgcctggt gctggcatta attgtctggc agcgtccgaa
    12241 tctgctgctg ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac
    12301 cgaagcatta atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct
    12361 gcgttccacc actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg
    12421 cgatctggaa gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga
    12481 cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgtcggga
    12541 agcggagctg cgtgcgcaaa cccagccgct gcgtaaagag attgcccgtc tggaaaaaga
    12601 gatggagaag ctgaacgcgc aactggcgca ggcggaagag aaactcggag acagcgaact
    12661 gtatgaccag agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa
    12721 atccggcctg gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat
    12781 gctgctggaa ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattc
    12841 agcagcagtg ctgaattcac ccctatgcgc ggctttagca attgtcatct acaaactatg
    12901 ctgccgcgtt tgtttcgtcg tcaggtgaaa ttcaccccgt actggcagcg gctggagttg
    12961 cccgacggcg attttgtcga tctcgcgtgg agtgaagccc ctgcacaggc tcggcataaa
    13021 ccgcgtttag tggtatttca cgggctggaa ggcagtctca atagccctta cgcccacggt
    13081 ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc
    13141 agcggtgaac caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt
    13201 tggtttttac gctggctgca acgcgaattt ggtcatgcgc caacggctgc cgtcggctat
    13261 tcgctcggcg gtaatatgct ggcctgtttg ctggcaaagg aaggcaatga tctcccggtt
    13321 gatgcggcgg tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa
    13381 aagggctttt cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgcg
    13441 cgcaagctgg cagcctaccc cggaacgctg ccgattaatc tcgcgcagtt aaaatcggta
    13501 cgtcgcatcc gtgaatttga cgatctaatc accgccagaa ttcacggcta cgccgacgct
    13561 atcgactatt atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg
    13621 ctgattattc acgccaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa
    13681 agtctccccc cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt
    13741 ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca
    13801 acgtatctgg aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg
    13861 aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta
    13921 cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg
    13981 tatggtcgga gctgcacgaa acggtcaata tcatgccgcg cagtcagttt cgcgaatagc
    14041 ctgcaaccgt cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc
    14101 caaacatccg gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct
    14161 cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag
    14221 ttttcaccgt tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg
    14281 gcggcatatc agctacttcg gccccgaggc taacgacttc ggcctgctgg aacaaacctt
    14341 cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgacga
    14401 agccgtaccg tggaatcagg taccggggac attcaccccc tggcaacctt taccggaacc
    14461 cactgatgta ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt
    14521 tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca
    14581 aaaactgatc cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcagt
    14641 agtgcgttca atggaagact atatcaacta catcacaccg cagttttccc gcacccatct
    14701 taacttccag cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc
    14761 gtcgctcgat gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc
    14821 ctggctgctg gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtacc
    14881 gggcggcaaa atgggtctgg caatggaatt aattatgctg ccgctggtgc aacgattgat
    14941 ggaaggaaag aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca
    15001 caacgggata gttaaatcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt
    15061 tttccagcat caacgccact gaacaatatt tctcagcaga gagatcaacc gcacgcgcaa
    15121 ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca
    15181 ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct
    15241 gacgcccttt ttgcaggata gaaaccacat cgatggcact gcaaccaccc gccgccatca
    15301 acaccatttc catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctggt
    15361 gaccagaggc ggattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca
    15421 tttttgctac tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc
    15481 aacggaaggc aacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta
    15541 tgctaaaaca gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg
    15601 tcacattacc gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg
    15661 caatccagag acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc
    15721 gcatggtgct tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc
    15781 acattcataa gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc
    15841 tgtactacat cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa
    15901 tgatcctctc ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg
    15961 gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt
    16021 acaaaaaatt tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac
    16081 agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg
    16141 tgacgggccg cattgcacag actctgctga atctggcaaa acaaccagac gctatgactc
    16201 acccggacgg tatgcaaatc aaaattaccc gtcaggaaat cggtcagatt gtcggctgtt
    16261 ctcgtgaaac cgtgggacgc attctgaaga tgctggaaga tcagaacctg atctccgcac
    16321 acggtaaaac catcgtcgtt tacggctctc gttaatcccg tcggagtggc gcgttacctg
    16381 gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa
    16441 ctatgcactt cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg
    16501 cgaattacga ttcggtctgc tcttctccct cgttcctgcc tgttgcaata ttgcgggcct
    16561 tgatacgcct cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac
    16621 ctgtagcttg ctgacacagc tactactggc aaaagatgtt cccctgccct ttttgctgac
    16681 cggattaacg ctggtacttg gcgtcactgc tgagctgggg ccattgcacg caaaattgct
    16741 tcctgcatcg ctgctcgccg ccatttttac cctcagtctg gcgggataca tgccggtctg
    16801 ggaaccgttg ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg
    16861 gttctggatc tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgcgaact
    16921 ggcagattat tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc
    16981 gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca
    17041 gcaaatgcat atgctttccg cgcaaaataa tactgattac aagcggatgc tgcgtatttt
    17101 ccaggaggcg ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt
    17161 gcaaaagctg gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt
    17221 cgccgctcgc ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacgcgttt
    17281 tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc
    17341 ggttgggcaa ttctgctact ggcatttcag ccgcatcgcc cgcgtgctgc gcacccaaaa
    17401 accgctctat gcccgtgact tactggccga taaacagcgg cgaatgccgt tacttccggc
    17461 gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt
    17521 gatgttaagc gttgccagcc tgatgggcac cgcgctgcat ctgccgaagt cgtactggat
    17581 cctgatgacg gtattgctgg tgacacaaaa tggctatggc gcaacccgtc tgaggattgt
    17641 gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa
    17701 aattcccgaa ggttacaccc tgacgttgat gctgattacc accctcgcca gctacctgat
    17761 attgcgcaaa aactacggct gggcgacggt cggttttact attaccgcag tgtataccct
    17821 gcaactattg tggttgaacg gcgagcaata catccttccg cgtcttatcg ataccattat
    17881 tggttgttta attgctttcg gcggtactgt ctggctgtgg ccgcagtggc agagcgggtt
    17941 attgcgtaaa aacgcccatg acgctttaga agcctatcag gaagcgattc gcttgattct
    18001 tagcgaggat ccgcaaccta cgccactggc ctggcagcga atgcgggtaa atcaggcaca
    18061 taacactctg tataactcat tgaatcaggc gatgcaggaa ccggcgttta acagccatta
    18121 tctggcagat atgaaactgt gggtaacgca cagccagttt attgttgagc atattaatgc
    18181 catgaccacg ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt
    18241 acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga ctggaatatg acgaaccggg
    18301 gagttctggc gatgccaata tcatggattc gccggagatg cagccgcacg aaggcgcggc
    18361 aggtacgctg gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat
    18421 ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg
    18481 ggattcgaag gcgtaatgca ggctaaacca tcggataagg cattcacgcc gcatccgaca
    18541 tcttttgcct gatgcttcgt atttacgccc caaccacctt cgccaccgcg tgggcgaaac
    18601 gttgcatccc ttcatcgata tccgcatctt ccaccaccag cgacggtgca aaacgcatca
    18661 catccggtcc ggcattcagc accattacgc cagcctctgc gcccgcatac aggaaatcac
    18721 gcgcccgacc tttgtactgt ggtttcagct ctgcgccaat caacagcccc ataccgcgaa
    18781 tatcgctaaa cacatcgtac tgctgatcga tcttctgcag atggtcaaca aaacgctggc
    18841 gtttcgcctg aatgccttcc agcacttcag ggggattgat gatatcaaac gccgcccccg
    18901 ctactgcaca ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg
    18961 cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt
    19021 tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat gtaagcaaac aaatcgccgg
    19081 tccgccccat cccgcactgc acttcatcaa acaccaataa tgcctgatgt tgatcgcaca
    19141 gctcgcgcaa gccctgcaaa aactctggcg tcgcagccgt cacgccgccc tcgccctgga
    19201 tcggctcaac caccaccgca caggtgtgat catccatcac tgctttcact gcgtggagat
    19261 cgttaaaggg aacgtggatg atgtctgccg gtttcggccc aaagccgtcg gaatattttg
    19321 gctgcccacc caccgaaacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa
    19381 taattttggt tttgaacggg ctatgacgca cacaggcgta atgacgtgcc agtttaaagg
    19441 cggtttcgtt agcttccgtg ccggagttca taaacaccac gcgttcggca aacgttgcct
    19501 caatcagttt acgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc
    19561 acagaatttc gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg
    19621 ccgtaactgc aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc
    19681 ggctgccctg accttttacc ggaataaact ctgccggagc ataaatcggc aggatcactt
    19741 catcgaaagt cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt
    19801 acgcataaac aaatgtgaaa ttataatcac aaaatatgca taaaaaatca ctaaatggca
    19861 atcagaaatc agcgatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata
    19921 ctttctggat ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc
    19981 tcgcgggttt cgctccaggc cgtcacgtca aagcacgctg gtaatgagtc aggttccacc
    20041 accagcgaat ggtagcgtgt cacggtaagt ggatttgcca gcccccgaaa tacgccctca
    20101 ccgctatgtg taatcggcga ggttttgccg tgcatgacct ttgcggcgcg cacaactttg
    20161 ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc
    20221 ccggcatagt gacgaataac gtcaagggag atcccggctt catctggcgt acaggggcca
    20281 ggtgagatga caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg
    20341 ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta
    20401 aaagaatcgt agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcgct
    20461 attttactca gattccccgg ctttgcttac cactttgcta aatatcatct gcagtgcggt
    20521 gagatccccc attgccccac tctgatttgc ctgattccag gcctcttttt cgataccctg
    20581 ccagctcagt tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag
    20641 accacttccc acccggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg
    20701 ctcgacgaac ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat
    20761 cagggcattg ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata
    20821 aatatcaact tcacgcagtt gccctgccca gtcgaaaata tcctggtaca gctggcgatg
    20881 gatagtgcgc aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg
    20941 cagcgccgtc atttcgtaag cggcctgttc cagccgctgc tgctgacgga tgttcagccg
    21001 gttacgcatg atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc
    21061 gctcatagtg cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga
    21121 ctaaaggcat ctcgacgcct tcaagacggc gactggcctg gaaattacga ttacgctgaa
    21181 gctcccagag acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac
    21241 gtgtccattt gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc
    21301 gggcaagatt gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac
    21361 cggttttgac ggcacattct ggtacggacc aacatcatga gtcggcacct gggaaatctt
    21421 atcggcaacg tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg
    21481 accatggtca aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc
    21541 tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc
    21601 ttcatttttg attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc
    21661 ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt
    21721 cacataatcg acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc
    21781 gatgttacca gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc
    21841 gggagaaaga gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat
    21901 gagatttcct taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc
    21961 aagaacaagg ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat
    22021 cactttttat tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat
    22081 aaaacattta aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta
    22141 tgccatttta tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca
    22201 tggattagct ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg
    22261 atgggaaata tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc
    22321 ttcctcaacg ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc
    22381 ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg
    22441 atgttcagcc acagcctggc gctgttctcg acggcgatgt tcattctcgg ggtggtcagc
    22501 ggcattacta tgtcgattgg tacattcctg ataacacaaa tgtatgaagg gcgtcagcgc
    22561 ggttcccgcc tgttatttac tgactccttc ttcagtatgg ctgggatgat tttcccaatg
    22621 atcgccgcgt ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg
    22681 ctggtgtatg tcgctatttt tattctgacc ttcggctgtg agttcccggc actgggcaaa
    22741 catgcaccaa aaacggatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt
    22801 ctctctgttg cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct
    22861 gagtatgcca aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc
    22921 tggatgtcat acatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg
    22981 caacgcattc tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc
    23041 ggaacaccag cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc
    23101 tataccacca tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt
    23161 aactttgtcc tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg
    23221 attgttgaac atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc
    23281 gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg
    23341 acctctcatt aattgctaat gccggacggc actatcgtcg tccggccttt tcctctcttc
    23401 ccccgctacg tgcatctatt tctataaacc cgttcatttt gtccattttt tgcacaaaca
    23461 tgaaatgtca gacaattccg tgacttaaga aaatttatac aaatcagcaa cataccactt
    23521 aaggagtata taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag
    23581 gtaggcggta atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc
    23641 ggtaacggta tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc
    23701 aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc
    23761 tcgtcttact tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac
    23821 gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta tcaccatcaa ccgtcaggag
    23881 aaggtgattc actccagcgc cggacgtacc gttttttatg acaagctgat catggcaacc
    23941 ggttcctacc cgtggatccc accaatcaaa ggttctgata ctcaggactg ctttgtctat
    24001 cgcaccattg aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa acgcggtgcc
    24061 gttgttggtg gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggtatt
    24121 gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt
    24181 ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac
    24241 acccttgaga ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc
    24301 agcgaactgg aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg
    24361 gcaacccagt gtggtctgga cgttgctccg cgtgggggta tcgtcattaa tgattcctgc
    24421 cagacttccg atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta
    24481 tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg ccgttgacca tattctcggt
    24541 agcgaaaacg cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac
    24601 gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta cgtttacctc
    24661 gacgaaagta aagagatcta caaacgcctg attgtcagcg aagacaacaa aaccctgctc
    24721 ggtgcggtac tggtgggcga taccagcgac tacggtaacc tgctgcaact ggtgctgaac
    24781 gctatcgaac tgccggaaaa cccggattcc ctgatcctgc cagcacactc tggtagcggc
    24841 aagccgtcta tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac
    24901 gtcactaaag gtgatctgat tgctgccatc aacaaaggct gccacacagt tgcggcgctg
    24961 aaagctgaaa ccaaagcggg tactggctgc ggtggctgta tcccgctggt cactcaggta
    25021 ctgaacgcgg aactggcgaa acagggcatc gaagttaaca acaacctgtg cgaacacttt
    25081 gcttattcgc gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa
    25141 gaactgctgg cgaaacacgg caaaggctac ggttgtgaag tttgtaaacc aaccgtcggt
    25201 tcgctgctgg cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag
    25261 gattctaacg acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg
    25321 cgttctccgg gcggtgaaat caccccggaa gggctgatgg cagtgggtcg tatcgcgcgt
    25381 gaatttaatc tctacaccaa aatcaccggc tcccagcgcc tggcgatgtt tggcgcacag
    25441 aaagacgatc tgccggaaat ctggcgtcag ctgattgaag ctggcttcga aaccggtcat
    25501 gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctac
    25561 ggtgttggcg atagcgtcgg cctcggcgtg gaactggaaa accgctacaa aggcatccgt
    25621 acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag
    25681 ggtaaagacg tgggtattat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac
    25741 ggcggcatga aaccgcgtca tgcggatctg ctggcagcgg atatcgatcg cgaaacgctg
    25801 atcaaatatc tcgaccgctt catgatgttc tacatccgta ccgccgacaa actgacgcgt
    25861 accgcgccgt ggttagaaaa cctcgaaggc ggcatcgatt acctgaaagc ggtgatcatt
    25921 gacgacaaac tggggctgaa cgctcatctg gaagaagaga tggcgcgcct gcgtgaagcg
    25981 gtagtgtgtg agtggactga aacggtcaat acgccgtctg cgcagactcg cttcaaacac
    26041 ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac
    26101 cgtccggcaa cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgagcc
    26161 agtggaaaga catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt
    26221 taggtgacga gcaggtagcg attttccgcc cgtatcacag cgaccaggtg tttgcgatca
    26281 gcaacattga tccgttcttc gagtccagcg tgctatcacg cgggctgatt gcggaacacc
    26341 agggcgagct gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agtgatggct
    26401 tgtgcatgga agacgaacag ttttccgtca aacattacga agcgcgagtg aaagacggcg
    26461 tggtgcagct gcgcggttaa tgttttaacg ggaggcgcaa tgcctcccct tt
//
LOCUS       DAZPLM010000037        25916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPLM010000037 DAZPLM010000000
VERSION     DAZPLM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.037"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(57..2015)
                     /locus_tag="ACHVC7_001316"
     CDS             complement(57..2015)
                     /locus_tag="ACHVC7_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR2115417.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            complement(2031..3086)
                     /gene="virB11"
                     /locus_tag="ACHVC7_001317"
     CDS             complement(2031..3086)
                     /gene="virB11"
                     /locus_tag="ACHVC7_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR2115418.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            complement(3129..4268)
                     /locus_tag="ACHVC7_001318"
     CDS             complement(3129..4268)
                     /locus_tag="ACHVC7_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFR2115419.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQEDNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            complement(4258..4959)
                     /locus_tag="ACHVC7_001319"
     CDS             complement(4258..4959)
                     /locus_tag="ACHVC7_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFR2115420.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            complement(5025..5759)
                     /locus_tag="ACHVC7_001320"
     CDS             complement(5025..5759)
                     /locus_tag="ACHVC7_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR2115421.1"
                     /translation="MENTNYYEHSKKEASKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            complement(5925..8282)
                     /locus_tag="ACHVC7_001321"
     CDS             complement(5925..8282)
                     /locus_tag="ACHVC7_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFR2115422.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAAGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            complement(8288..8608)
                     /locus_tag="ACHVC7_001322"
     CDS             complement(8288..8608)
                     /locus_tag="ACHVC7_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR2115423.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALIAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            complement(8679..8969)
                     /locus_tag="ACHVC7_001323"
     CDS             complement(8679..8969)
                     /locus_tag="ACHVC7_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR2115424.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFS
                     FGGVCALCYLIYNVIMAFMEKKSWSDVGMALVYCSLAGGALYAGNWALELFK"
     gene            complement(8969..9553)
                     /locus_tag="ACHVC7_001324"
     CDS             complement(8969..9553)
                     /locus_tag="ACHVC7_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR2115425.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEINPPNDEEKTSPDWDVFGDFS"
     gene            complement(9574..9972)
                     /locus_tag="ACHVC7_001325"
     CDS             complement(9574..9972)
                     /locus_tag="ACHVC7_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115426.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADIIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            complement(10091..10528)
                     /gene="pilM"
                     /locus_tag="ACHVC7_001326"
     CDS             complement(10091..10528)
                     /gene="pilM"
                     /locus_tag="ACHVC7_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR2115427.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFVH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            complement(10534..11769)
                     /locus_tag="ACHVC7_001327"
     CDS             complement(10534..11769)
                     /locus_tag="ACHVC7_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR2115428.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALSPAKPISSNNHQTQTAQKTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKALKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKSLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            complement(11772..12071)
                     /locus_tag="ACHVC7_001328"
     CDS             complement(11772..12071)
                     /locus_tag="ACHVC7_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR2115429.1"
                     /translation="MKSIILSMVAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSE
                     CKSAEKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            complement(12138..12773)
                     /locus_tag="ACHVC7_001329"
     CDS             complement(12138..12773)
                     /locus_tag="ACHVC7_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115430.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            complement(12926..13180)
                     /locus_tag="ACHVC7_001330"
     CDS             complement(12926..13180)
                     /locus_tag="ACHVC7_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR2115431.1"
                     /translation="MNKSRNAIIASSIIFASLMLVGCKEKIYSVEYYSNNISEATKTL
                     EDCKKGTITDQNCDNARAALQQKQDSEYKKKVSEMRRRLD"
     gene            complement(13182..13823)
                     /locus_tag="ACHVC7_001331"
     CDS             complement(13182..13823)
                     /locus_tag="ACHVC7_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR2115432.1"
                     /translation="MKDKVLSFLFAGIITITSPSIFASGIPVVDVAAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNVAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLENERKAQYETYVRQKRRDLS"
     gene            complement(13829..14824)
                     /locus_tag="ACHVC7_001332"
     CDS             complement(13829..14824)
                     /locus_tag="ACHVC7_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR2115433.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPTFFAAIALYVIY
                     LIYEITYAQRDVLMSEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNMLSVTLNDFKNNELDTLEWYSFTDQLYIYLIWGVGYVGGLLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFSLFASTRNMFSAWVGSCLNYILLNLFYSISFSF
                     VISFVEQTVPSSGNITLTTVVYFFMVTVISIFLVEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPFFNAGSRSASALVQGLNRGSKLKAG"
     gene            complement(14828..15085)
                     /locus_tag="ACHVC7_001333"
     CDS             complement(14828..15085)
                     /locus_tag="ACHVC7_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115434.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KEESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            complement(15082..15384)
                     /locus_tag="ACHVC7_001334"
     CDS             complement(15082..15384)
                     /locus_tag="ACHVC7_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115435.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKNIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            complement(15647..16303)
                     /locus_tag="ACHVC7_001335"
     CDS             complement(15647..16303)
                     /locus_tag="ACHVC7_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115436.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTFLSDTGSDL
                     AEPVTTTTATGKIQSILGGMAEMVSILGTVVPFLVIIIAMIWFLFFSSKREKEELTRV
                     EREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALADESNNVRG
                     "
     gene            complement(16488..16931)
                     /locus_tag="ACHVC7_001336"
     CDS             complement(16488..16931)
                     /locus_tag="ACHVC7_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR2115437.1"
                     /translation="MLWFTLFLLCIALEIITGTGWLLLISLGALSSAIVGFFLPISQE
                     ANICFFASISILASIIKFFYDKKHKKLDTLLVNTGHSRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSESKE"
     gene            complement(16994..17947)
                     /locus_tag="ACHVC7_001337"
     CDS             complement(16994..17947)
                     /locus_tag="ACHVC7_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR2115438.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDIPKQEVISKDNANVTIDAVCFVQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPKELTEAMNAQMKAERTKRARILEAEGIRQSEILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            complement(17993..18187)
                     /locus_tag="ACHVC7_001338"
     CDS             complement(17993..18187)
                     /locus_tag="ACHVC7_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR2115439.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIHPQ"
     gene            complement(18180..18632)
                     /locus_tag="ACHVC7_001339"
     CDS             complement(18180..18632)
                     /locus_tag="ACHVC7_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFR2115440.1"
                     /translation="MARKLKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            19764..20792
                     /gene="repA"
                     /locus_tag="ACHVC7_001340"
     CDS             19764..20792
                     /gene="repA"
                     /locus_tag="ACHVC7_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR2115441.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDNYKALGGQLGHAYNRLVKKDPVTGQNTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGDVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRR
                     KKAAERKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            complement(20890..21261)
                     /locus_tag="ACHVC7_001341"
     CDS             complement(20890..21261)
                     /locus_tag="ACHVC7_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115442.1"
                     /translation="MAEYLQRFFVGGLEGLCFSLGRSPYARREAVGGTFNILSGAALA
                     AASEGIFVIIHSCFKDKYFPCGEATQGIKEQGLRIYCGFDVLCLLLSEVIPLRVFVDN
                     RRRFYSDTLLRPSKNCQSGTK"
     gene            22143..22295
                     /locus_tag="ACHVC7_001342"
                     /pseudo
     CDS             22143..22295
                     /locus_tag="ACHVC7_001342"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            22240..22362
                     /locus_tag="ACHVC7_001343"
     CDS             22240..22362
                     /locus_tag="ACHVC7_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2115443.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGNREVTASLAYESNGK"
     gene            22512..23108
                     /locus_tag="ACHVC7_001344"
     CDS             22512..23108
                     /locus_tag="ACHVC7_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR2115444.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(23126..23410)
                     /locus_tag="ACHVC7_001345"
     CDS             complement(23126..23410)
                     /locus_tag="ACHVC7_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR2115445.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(23484..24149)
                     /locus_tag="ACHVC7_001346"
     CDS             complement(23484..24149)
                     /locus_tag="ACHVC7_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFR2115446.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
     gene            24748..25077
                     /locus_tag="ACHVC7_001347"
     CDS             24748..25077
                     /locus_tag="ACHVC7_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115447.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIEEFETAYKGSPEEK
                     HEFTSYY"
     gene            25657..>25916
                     /locus_tag="ACHVC7_001348"
     CDS             25657..>25916
                     /locus_tag="ACHVC7_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115448.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKFD"
BASE COUNT         6942 a         5460 c         5623 g         7891 t
ORIGIN      
        1 attcatgttt aattacctta tggaatatgc aaaatactat ttgctggttg cgaggattat
       61 tcagatacgt ctgttttatc gtgattatca aggtctggat tagtaatcac attgcctccg
      121 gcaaccatga ctttttttcc tccagcttta gggagataaa tttctgcaat ttttgctatt
      181 tcatccatga cttcttgtgt aaacaggttt ggaatgtcaa catgattgtt acgggcgata
      241 tccacgcggc gtttaaactc tggttcttca aaccagatta ttttattggc aataaatggt
      301 cggctaaatt cactggttat aatttcacgg attgcaatat ttctggcttt attcttctta
      361 tcccggagag acacaatttc ttcaggaaga agaaccgggc gctctattac atcatcattc
      421 cgtgtacgtg aacttcctcc cttaccacca ccactggata ttgagcgttt ggatattttc
      481 atatcacgca ccccaatctc ttctgaaatc tttttagcca gagcattttt tgatttcggc
      541 ggatagtaaa gcacaacagc actgttttca gtgaatgtgg tccatccttc ctgaccgtac
      601 atatcgcttt tttgcccttg tccttcattc tgaagaatga acataaagcg aaggttataa
      661 cctgctgtaa aaccaacagc gcgttcaatg acctccgatt tacccattga tgtaaattca
      721 tccaggagaa taagacactg gtatttcagg tccgggttgt gttccggtag ttcacgacag
      781 ttttcattaa ccagtaatga gaagaaaagg tttacaattt tctcatgagt gattaaagca
      841 tccggtgtca gaccaagata aattgacatg ggctttttac gaatatcccg gatatcaaaa
      901 tcactgaaat tcgtcgcctc agcagtaata gggttgctga agatgctcag cggtgatgaa
      961 aagtttgttt ctatacttcc ccttgtgcga tctggtgctg acatgaactt aaagaagaat
     1021 gattttgttt tatcgctaat ccagctacgg ttttcaattt cctgtcccat ccatgcggcg
     1081 agatccttgc cgccatcggg aaccgacgtt ttcagtatgg cgctgatgga gacaagaacg
     1141 tccggtacat tatgaccttt ggctttctgt tccaggtgaa aacgctcctt gtccagcagg
     1201 taaagtccta acccgacaaa aaggtttcgt gcagaatcag accatatagg atcatcactt
     1261 gccgggataa ggattgcggc aatttttgcc aggtccgttt cacgaagtaa atcactgcga
     1321 ctgacgcaat ccagaggatt ccagcggtga gagcgtatct gtcctttaat tgcctggtcg
     1381 atagtttccg cgtatccggc gggcgcaaag agaaagcatt cttgccctaa ttctttttgc
     1441 ctgaatccgg ctgataaaaa ccagttttca agtttgatat ccagtatgac catacttcct
     1501 ggatagttta cgcagttggg gataacaatc cccacccctt taccagaacg tgtcggggca
     1561 taaagaatta aaaattgttg tccggcgaaa taaataaatt gttttttgta tctccccttg
     1621 aacattttac cgatgagtat tggtggatgt ttgtaaggtg attttttatc cgggaaaaaa
     1681 cctgatttac tcaggtccat atcagttgcg agccgggcgt caccgtagat gactttttta
     1741 ggcataatcc caatcacgac aattaacatg aatatggcaa agaatattaa tggaacgctg
     1801 aatccaaacc atgccataaa aacgagtttt ttatattgtg ggttgccaga aaatgttgag
     1861 attacagaat ggaatgtatc ccacttgagc aaagacatat caacggatga atatcttaat
     1921 gctgtgtatc caccgagata gtttgaggct ataagcacac ccacaagaag cagtaacagg
     1981 aagagaatta acccccccgt cttttttgca tccatagtta tctccagaat ttacattgtg
     2041 ctcaaaaagc gatttttttc ttcgggttcg taatacagtt cagtcattct ggtatggttg
     2101 aaaaaaagaa tgacatcaat tgatgttttt atcgtgcgca tgatgaggtc catatcaaca
     2161 gtcataccaa catcagattg ttttacaata cttgccagtc gtgaaaatga ggcataagta
     2221 ttattagcat gaatggtact gattgagcct tcatgccctg tattaagcgc ctccagataa
     2281 ctccatgctt cgttacccct aagttcagca agaaagatgt gatctggctt taatcgcata
     2341 cacgcttcaa tgatttcctt cgcttcaacg gtattttttt tgtagaaaag ccggatgtgg
     2401 tttggatgca gcggcagaga catttccggt acatcttcaa cggttatata acgccgttca
     2461 ggtggaaaga tatctgcaat tgcctttgct atagttgtct taccggagcc agtcccaccg
     2521 acgattaaaa aattaagacg gtctttaact gcctctctta aaaattgagc tttagattga
     2581 ccatctggac gccgggataa ttcatacagg tagcgttgcc gttcagtcag gatggcttcg
     2641 tgtttcgtcg caatcctgac attatcaaaa cgccctgttc ggacataatc atctatagta
     2701 aagcgcgtta atgatggctt acggatagat atcacaacgc tgttattttc tgttgcaggt
     2761 ggaatgatga tttgtccacg ttccccaccg ggaagaacta ccgatgcaat gggattatca
     2821 tggctaagtg gtatttttat ctttgataag ttcgtcagag ttttggcaag tgtcattaaa
     2881 ttatcaaaag tcgcgtctgg cgcatcttta gattcccagc catttttccc ttcaaaccat
     2941 attgttcctg gtttattcac agatatttct gtgacatgtt caatattaag aacagactgg
     3001 atacccgtca tatccagata tcgtcttgcg gttttactgt tatctcgtgg ttgtgatgta
     3061 ttactggtat ctctgatgtt attcatatca attgctttac tgaattactg aattactgaa
     3121 ttactgaatt actgaagttc atatactgat gaaaagtcga tgtttcgcgg aacaataaca
     3181 gacaatacag tcccctgatt aatatatgcc gtggggggaa tatttatcgt attgtccagc
     3241 gttgtttttg ccatttcttt tgttgcgtca gatgtgtttt cgtaggtgat attgctgttt
     3301 gagcctgttt gttgcgtgct gtttttcaga atatctaaac catcaccaag taatgacagc
     3361 agcaatgcac caccaaaacg ttgaccaaaa tgattatcga cccatgcagg aaggccggaa
     3421 gcgcccagac tgtcagtgcc aagtgcgcca atgcgcacat tgacattttc atctttgagc
     3481 gttgtccagt taacgaacac acgggctact ccctgtgtca tgactttatt ctgctccccg
     3541 atcagtaatg ctcctttacg ggcaagaagc acttcgccgt tatccgacca gacgtctcgt
     3601 gttaactgac acattgtgat accgggataa gaggttacga ttttcgtttt gagcacacag
     3661 gataatgctg ttcccgcaga taacagatat cggcggttca gcacagggga tacctttccg
     3721 tctgcgtact gactgccctg taaatcatct ctttcctggt ttttttcgcc tggttcctga
     3781 ttgtcgatct tcaccattgt gtcgcccatc agttgtcgga cggattttgg caatggttgt
     3841 ggttggtttg gatctccact ggcggtttgg ggagtgccgt tggctatcac attgacggct
     3901 gtttgatcgg ccttttgtga tgctggcgct gacggggcat tgtcttcctg tttctgttct
     3961 tgctcctcct gtgctttcct gcggttagcg gcatcgagtt tttccttttc ttcgatgttt
     4021 ttcatcattg ttgtcagtga aacgccattg ttggtatttg tgaccagtgt ctcgtcagac
     4081 ttctcaagag gtgtttgctg aacgacctgt tctcgtgaaa gaactttaaa caagataatg
     4141 atcacaaata acgcggcaat cagtaatatg atgaggatta atattttttt tggtgctgct
     4201 ttacctttta cttcgataat tccgcgattt cccgcgtcat tatttttttc atcatcttta
     4261 tttttcatag ctgaacttct ctactcgtgt ttttccgttt gacgtacctt ttttattcca
     4321 cggacgatta acgattgatg atgttctgat atctgcaacc tgatcaccca ggcgcaaacg
     4381 gagattttca gccacgtcat agtaaaccat tgtgttttta tttctgtctc cgttaaccag
     4441 atgttcattt ccatcggcat caacacgata aaggaccgga atttcctgtt tattagtcca
     4501 tctcatgcat gtaaattcgc cattatccca tacctgataa gggaaaattg atttgtcccc
     4561 cttgagttga taatgaccat taattattcc accatcagtg catggaaatg gcttttcagt
     4621 cattacggta gctgacggtg gtttcggata attaaaacgc agaatatacg tgggttgttt
     4681 cgtggttgat atcagataaa gaggatatat atgcttattg gtctgaacgg taacgtttgt
     4741 atccggttct tctgcttttg gacgcagaaa cagagtgttg tccctgaccg atactgacca
     4801 ggcttctgga tcaccaattc cgacatcttt tattgtttcg tcttgtccga acttaattgt
     4861 tgtttgtgcg cctgctttta cgcgcacatt aaatacatcc tgctcgttat aatctacggt
     4921 ctgaatacgc ccgtctcgtt ccgatggtgt gccgtacata gctgctaaag caccaggtac
     4981 ttgtagcatc gacaaaagaa ctatcgcagt agtgcgttta agcatcattt tataacctct
     5041 ggatcaggtt ggtagctcag aacttgcagt cccagcggat tcacaaggcg ctctttttct
     5101 gttttgatat ctttatccca gtcaaaagaa atcgtcgaaa tccactctgt tgctctgtat
     5161 ccgggggcgg gtttaccaga taaatccagt accatttttt tgaaacgcac ctgcgccata
     5221 tccttcctta gaaggattac tgaattaatc tggaccttaa ttttgtagtt gtttttcaga
     5281 atgttcagtg gtgaggaatc agcacgaatc atggtgtcat aagctgaaaa caccttttgg
     5341 gaagacattg tttttactgt atcggcctgt tcctggatag tctgccagtc gtatgcttca
     5401 tagttaatga gatattttga caggaaatat ttagtctcaa catcctgata actttcctta
     5461 gcatcagata attgaggggc aatatcagta tatccagtgc tgttatccac tcgcacaagg
     5521 aacggtacag cggtttttaa tggagcaagg gaggctattg cgaaaccaag aatgcaaatt
     5581 acaacaaatt caccaatggc gagaatggta aatgtttttg cctttttctt tatgatttca
     5641 atttcactgc gctcaaagtc acgtatggcc ttgaaataat cctttttttc gttcttttct
     5701 tcaaatttct ttttggaggc ttcttttttt gaatgttcat aatagtttgt gttttccatt
     5761 aataatcacc tgctttgatc tgttcaattg ctgtgtttaa ctcaaaccat cccccttttt
     5821 tctgcacagg attgggggtg tgagaacatg cgctaattaa tagcgcggag gcaattatga
     5881 gggggatttt catattgttt tccgttctgt ataaaacaga gaaattaacc gttcaggcgt
     5941 ttcaggagtt ctggtatcca gatatcagga gctacatctc ctttttcagc aataatctca
     6001 tcaaaaatag acaggccagt ttcagagcca gaaagcacgg ggataaactc atcgaatcca
     6061 aacaaatcca ttttggcgaa aacagaagag ccagattgct tgatgagcat ggtccttgac
     6121 tccttcgtca gctctttgag tttttcaaac tctttttcag taagcccaat ttctttatat
     6181 ccttcccatt ttgcatcggg attgggcagg agaattttcg ttgcggtttg ttgtaccagc
     6241 gccgcaaaaa ttgcgcagtt tatggcgtct tccggggact gtgatgttaa ccacatcatt
     6301 tcacctttca tacgccccgc tttcagtgcg cttttaatca tgctttgtgt catgggaaag
     6361 tttgctggca tccagaactc ttcaataatg gagagcatca gattgccgcc gcgttgcatg
     6421 atttctttgt agaaaaacag gaccgcgagt aaaggttcac aggcaggatg aataccgttt
     6481 tttgtgtcca ggacaacggt tgtatcaaat cccacttttt tatgatgcag tggattgaag
     6541 gtattccggg gggaatccac cgcccaggca tattcgccgt tgtcgcacca tttggcgaga
     6601 cgcgtctgaa gttcaggact gactatatcg agaagaaggg ctgtccggcg ttcttcgaca
     6661 ggcattcgca taatgctttc cacggcagcg tttaattcaa tgccatgttc atcagaacag
     6721 gggttgccct ggttgtctcg tgccagaacc tgtgtccagc gtttcagaaa tgcgagaagt
     6781 ctgtgccaga cgggagattc cgggcctgct gccagttgcc acggattgca gcctgtataa
     6841 atgccttcct gcaacgtgaa atagcttcct ccgtatgcac ggacaaaaag ctcagtagaa
     6901 cggttgaaat ccacaacaaa cattaaaggg tcaaagcgtt gaagaaaacc gctggctgct
     6961 gcttcaaatg ttgttttccc tgtgcctgtt gcgccaagaa tcattccatg cccggcaatt
     7021 ttttgtccgg taacattttt ttccggtggg ctgtaatgcg tattgaacca gtaaatgctg
     7081 tcagacaccg ttttcaacgg cattatggcg gacccatcac caataggatt gcctgatttt
     7141 ttcccggagg aataattgtg aaaagacatc agacaggcca ggttggttat tgttcttcgg
     7201 gtatccagtg gacgcctctt attcagcggc atatgactga aaaaaacaaa aggtgatgcg
     7261 agtgaggcgc gactgaagcg gaatccctta cccgaagtaa taaactcagc agagagcttt
     7321 ataccattgc ttctggcctg atctggtgta tcaccgaata ccgttaatgc gcagtgtagt
     7381 gaaccaaaca acgtaattcc ggctgcgacc gcttctttac cagcttcgag ttcttcctgt
     7441 tgtgttttgg ctgcatcatt agctgattgc agtttgttta attgcgaatc aatgttcttc
     7501 agggtttttg tgggtgattc aaaaataaac gattgagtaa gaataaattc atagggttgc
     7561 ttgagaagaa aatcccattg tccgggggtc gtttctatag ggaaatcctt caaaatataa
     7621 ttcgttgcaa actttttact atccgattcg ttgttacgca gttcaagaac atcagcacca
     7681 aaataccatt cagaatcaca aatactggat gataagggag tggatgataa aggaatcatg
     7741 ttatgttctg tatttagcaa tagtgataaa tagtctgcca cctcactaat ataagtattg
     7801 tgaactgata aaacagaagc gttaaatggc tgaagtgctt tttgcgcctg ttgtgtcatt
     7861 tcattcatgc gctcgattcc ggtatcaaca tcatcgtagg gaatcccaaa tgttaaatac
     7921 catgttgatt tgtagaatgc gctggatgtg aacagagcaa ggtatttttt tgaaaacctg
     7981 cttaaaaaag cattgtcaaa attccattct ccatcaatgg ttattctttt tttgataaga
     8041 tgagtccata aatacaggtc gccttccttt cctaacccaa tcaggaagtt tttaacgctg
     8101 ttaaataaat ttgtaagaac attatcgttc tctgtctcaa atggaatgcc tgctacaaca
     8161 agtgaagcca gcattttcct gtcagaagta aagataatac tgtctgttat atggaagcga
     8221 taatcgggat attcacgagc aacatccata ttgtcactgt tttttaattt gattttgtgc
     8281 atatatgtta cctgacagat gaataaccaa taattaaatc tctgtggcgg atttttaaca
     8341 aaagccccat aaggtttaaa cggatgactc ttagtgcgtt tgagtcattt tcacaggcca
     8401 gttttactat aaaaagaaac agcgcacata tgacaggaat aattatccct tttatacccc
     8461 aaaggaaaat agcggggaag cctgtcagaa cagcgaaaaa agccgtgaac aataacaaca
     8521 ttaagggtat accagcaatt aacgcaggac ggttaaaccc gttataagtc atgaatgact
     8581 cactgacatg ttcatttact ttgtccataa ttcaccatga aaaaagccgc aattcacaca
     8641 taaggtaaat atgcggcaca gttgtatata tggattactt atttgaagag ttccagcgcc
     8701 cagttcccgg cgtaaagtgc accgccagcg agggaacaat agacaagcgc cattcctaca
     8761 tcagaccagc ttttcttctc catgaatgcc atgatgacgt tatagatcag gtaacacaaa
     8821 gcacaaacac caccaaagct aaatgcccat gtcttaagtt ctgtcatggt atttgttgcg
     8881 gtgtcgagtc cacctgcata ggcaacggag gcagttagtg acaggaaaaa cgccagaata
     8941 aaatactgca aagttgattt gactttcatt aactaaaatc tccaaaaaca tcccagtccg
     9001 gtgatgtttt ctcttcatca ttcggggggt ttatttctga ttcatcagtt ttttcacgga
     9061 ttgatggcac gatatagtta ttcgcgttac gaataacccg ttccagataa gaagtgttgt
     9121 taaatttctt ttctttaata aatcctgttg attcgttacc cgaataatac aaagataaag
     9181 cgcggagaat tttttgatta ctactaccct ccttttgtct cagataattg tccgtcagga
     9241 ctgccgctcc tgcttttata tttgtacacg ggtcaaaaac tgttttattt gtcagattga
     9301 gtgatgggaa atttttgctg tatatctgtg tcagacctgc actaaacctt ttattttctg
     9361 ctgttaattt ttctgcatat tcaatagctc ctttttcatc tttaaaatac ttacttgttc
     9421 catcagaaac attcgctatt acatatggat tagcccctga ttcagtcata atcagggcat
     9481 tcatcgtatc gggtgataca tcaggggcac actgacttat cagtgctgcc agaacagccg
     9541 ctgataactg cataagggat ttatctcctg atattaaatt cgtgaagaac gttttttcct
     9601 gcgggggcga cgaacgatac tgtccagctc attagcgccg gaaagaagtt ctttcagcga
     9661 gacaattttt tccctgacag gtataaggga ttttgccccc ggacattgtt caaagtctga
     9721 tttgtacagg tcgtataaaa caagcgtatg ttttacatcc tgtgacagat aattatgttt
     9781 attctggatg ttaccttgtt cgatattttt gataatatct gctgaacgat gaattaaatc
     9841 tgtgtagatt cgggtgtttt taagaggatt tatttttata tattcagaag caagctgaat
     9901 aagattgcat cgtaagataa ggacttcacg agtggcaata tcaagctcgc tgacatcatc
     9961 attatcctgc atgggtatct ccggtaaaga ttttctggac tgaagtccgc gccgtagcga
    10021 tatatccggc ccacataatg atgtgtcgga tatgcagact ccagttcaga atccccttgt
    10081 caggggatat ttagttaatg agtacaatag cctcttctgg aatggaggaa ggaacgggaa
    10141 cgcccatatc attcccctga ttatcgacaa tcttcttatt ttttactctt cctaacattg
    10201 ctgaatgttt tgtttgctgc tgcaaagcag acatcagacc ttctgtatcg gcagaccaga
    10261 tataaactcg cttaccgtaa ataatatgat gaatatcata aacgggtgtt ataccgattt
    10321 gctcagatgt taacgttcct cctgaattac gtttgtctgg atatttataa agatagtcat
    10381 tgataacatt tgcgaaatgg acaaattcaa ttgcttcctg ttttgcagga agcaacttca
    10441 ttcgctgtat ggtttggtta ttgttatctg tcagatagta actgcctatt gacaggaata
    10501 tcaggagcaa gctgaagaac caacccatag cgattactcc tgttgtgtgt ctttaattgt
    10561 gattacgcat tgtgcaatat tcctgaatgc gtataacggt ttatctgctg aaagataaag
    10621 catcagcgta tcttttaatg cagattcaaa gtttccccgg aaaataagag gagcatctat
    10681 tctgtagttc acaggagtat cccatacaag attccaggtt gaattaccac acggcgaact
    10741 tgacgcccat tctgtgaggc catcccgcag cgtcgaacct tttttaagat tccaggtgag
    10801 tttttctgtc tgtttttgcg gaacggtgac aggcgttagc gatttttgag gtaacttgtc
    10861 agaggaaacg ggtgtcctga caggagatgc aggggttttg tcaggagata caggtgcttt
    10921 ttgtgatgtt tttcctttct gttgttgaaa atcaatcaga agatgtcttt tttcggtgtt
    10981 cttaattacc gtcagaccgt agttgcgaag cattttatcc aggacataag gccattgatc
    11041 gttgccctgc caggatatcg tgcgacggaa agttttcgtt acctccgggg atatttcagc
    11101 cgtccagtcc gatgggacaa tttcttttaa tgctttttgc agcgagcctt taccatcatg
    11161 gcgaacaaag taaatttgct tttcaggtgt tgatgggcct gtaagcgtgg tggccggaac
    11221 agcgttgtta gcgttttttt tcagaaaacc gctttttgac attagcggtg gttgttcagg
    11281 acgcggtttt gtctgctgat cagtcttttg tgccgtttgt gtctgatggt tgttgctact
    11341 gattggcttt gcgggagaga gtgctggttg ttcagaatgc tgtttagttg ctcctttggc
    11401 tgctgttttt atctcaccag atgaagttat gctgtttgtt gtcggcttca gaaaactatt
    11461 attgctgccc tggttttttg gcgctgtcgc tgattgctca gaacgtggtt ttacggtttc
    11521 tgcaatacga ggcgcgggag ttacgtttat ggcttccttt ttgagataat tatcattgct
    11581 gtattgaaca gtgctgtttg gctgtgacac gggctgctga aggatcaccc cgttatagta
    11641 tgtgtctgct cttacctgct caattgtggt attcagttca taccaacctc cttttgcctg
    11701 tgcgggttca ggggcatggg aacacgcatt cagcaataat gtggtgggaa taataatgag
    11761 ctttttcatg gttaacccca ggaacgtccg aacttactca tgatttttga gatcgccgca
    11821 gggtctgcgg tcgtgcattg attaagaaac gcttttcggg catcaaaggt tttgctccag
    11881 cgaatagatc cctttttctt ttttatgata ttaaagaagt ccttttcggc gcttttgcat
    11941 tcgctcccgc cgccgctacc gatagcttta ccgtacagac aaagaacgga agcacacgga
    12001 tcagacgcat atacgggggc agagagtgcg atagcacttg cggcgaccat cgataaaatg
    12061 atggatttca tggtgttcct ccatatttca gacaaaagaa aacccgcacg aggcgggtaa
    12121 tttaaatatc aggggaatca acgcacgtgt ggcgtaatga tgacaatccg gttctcactt
    12181 tggctatcag cattaactcc agtaaaacca ctcacgataa ggggctggtc cggtttgagt
    12241 aaaatattct gctcgacagt ctgtttccgt atcgggtcag tggttgtgag tagcagatgg
    12301 aaatttccac gctcaatctc cggtgctatc cgcattgtga agccagcagt ccgatctttt
    12361 tcagccagtg tagtgtcacc ggatattgcc tgacggggga tcagttccgt ggcaggttcc
    12421 cagcttccgg cagatgttgt ttgtgattca acgcacgggg tactctgcat tattttgcgg
    12481 attgtttcgg aggatatcgc ctgaccgtca tcaaagaatg cactacggaa aatcacgttc
    12541 tgatttttgg caaacaagcc tggatgccgc tcgctcagac tttcgtatat attcatacca
    12601 ttcaaacaaa caggagaact gtccggcagg tctgttcgca cggtatcaat tttcaacgtt
    12661 atctggtggt catttgaggt taacggtgac tcaggtactg gttgtgtttt gctatccgcg
    12721 cagcctgaca gtaatgcggt ggcggcaacc atcgataaaa tgatggattt catggagttc
    12781 ctccatattt caggcaaaag aaaacccgca cgaggcgggt tttttatcat ggcataagtt
    12841 tatttattgg agcgtccaag catttaactc atgatttctt ttcgttttag atcatagagt
    12901 gcttcgttag cattttatgt attccttaat caaggcgtct tctcatttca ctaactttct
    12961 ttttatattc actatcttgt ttttgctgaa gtgcagcacg agcattatcg cagttttgat
    13021 cggttattgt acctttcttg caatcttcta aagttttagt ggcttcactg atgttgtttg
    13081 agtaatattc tacagaatat attttttctt tacatcccac taacattaaa ctggcgaaga
    13141 taattgatga ggcgataatt gcatttcgtg acttattcat atcagcttaa atctcttctc
    13201 ttttggcgaa catatgtttc atattgagct ttccgctcat tctctagttg ccgttcagca
    13261 atatcatatt gcttcaactg tagctccatt gtcaattttt ctgtattcaa ttggttagat
    13321 aagtcagctt ttttttgcgg agtatctgct gacgcgaact ccttctgtaa cgtggttaat
    13381 ctcttagccc tttcctgcat ttcattgttg aaattttcat agaatttgat cttagccagg
    13441 atcgcatcat atttttgttg cgtctctgca acgttgcttg ataagtcata ttgcgatcgc
    13501 aaagagctaa gatctgattt tgcactattt gttattgttg atagagaatc cttcatatag
    13561 ctggatgcgc tatcaaagcc attgctgaaa tcagtgaagc cttcgagtct tttcttctgt
    13621 tcttcagcgt atcttatggt ctgttcatat tgttgcttga gttgctcaag ctgtcttgcc
    13681 gcttcagcag cacgatttag cccttcttca actgttttag ctattgctgc aacgtctact
    13741 actggaatac cagatgcaaa gattgatggt gatgttatgg taattattcc agcaaagaga
    13801 aatgataata ctttatcttt catgttaatc accccgcttt aagtttactt ccacggttta
    13861 atccctgaac taatgctgat gcagaacgac ttcctgcatt aaagaatgga tttggcattt
    13921 tgctggcaaa accattagtg aaagatcgga gtcctgatgc tcgcataaaa ccggatgcaa
    13981 ccttacctcc aaagccatta gcggctgatg taaggccatt tatacctacc ccgccagtaa
    14041 gagttgagca gagagtacct acttgctcaa caaggaatat agatataaca gtcaccataa
    14101 agaaataaac tactgttgtg agagttatat ttccgcttga tgggactgtt tgctctacaa
    14161 agctgatgac gaagctgaac gatatactat aaaaaagatt tagcaatata taatttaaac
    14221 agctacctac ccatgcgcta aacatatttc ttgtcgaagc aaatagagaa aaacagataa
    14281 ataatattcc ggcggataag agaatgccaa ccatgaatgt tgatagagtt agaaatacag
    14341 tggtgtaata aataagcagc agcccgccaa catatccaac cccccaaatt agatatatgt
    14401 atagttgatc tgtgaagcta taccactcca gagtatctaa ctcattattc ttaaaatcat
    14461 ttaacgttac tgaaagcata ttccacaaat tatcaacact ggttgcagtt cctgaacctc
    14521 cagtaacagc agcagaaagg tcggagccag cgtgcattac aaaaggaata acaaactggc
    14581 tgtaataagg cgctgaataa gtgaatgcac caaccagggc gaatgcaccg atattcttag
    14641 tcacctcgct catcaggacg tcacgttggg catacgtgat ttcatatatt agataaataa
    14701 cgtataaggc tatagcagca aagaatgtag gagatattgc ctgtgcgacc ttagcggcgt
    14761 tcgtcgccgc tgtggattca acaatggttg ttactttctc aagaaattct gaaacaatgt
    14821 tcatagctta gtccttctgg cagatgaaga gttcaccaga ggcactaata tttggcttca
    14881 gcttaattac caggtctgtg ccgaaaaaaa ttgcttctcc aattttttgg aaatttaatg
    14941 tggattcttc tttattgtaa ctataaacgt tgtgggtcgc tgtcgatgtt ttggttttgc
    15001 gtggtgtaaa cgctttttta atgatgtaat cgagagcgtg gctattatga gatggtaaag
    15061 tgattaaatc gtcgtttttt ttcataatgt agttctgatt tttgaggttg aagataattg
    15121 tcggtcaatg cttttgattt tttttcgtat ctcaattgga ttcgcctcag ataagttagc
    15181 aatggataaa gccagttcga tttgtaaaat tttccttgtt tcgatgagat tgatttttac
    15241 ttgtggtata tcacggaaga caccacagcc ggtaattgct tttgaaacga cacgttctgt
    15301 attcgctatg tttttcttta ctgttcgagg ggggacataa cgtgcacggc gaagttttct
    15361 cagattagct tttgcttgag tcatatttgt atgccctgaa taaataaatg ctttcttcaa
    15421 agcctgaata agcataaacc aggattatgg tggctaaagg caaagaaaag gtgaaatgaa
    15481 taacggcatt cagaactgaa ccggttaatg tatataagac aaacggtgct gaaagtatga
    15541 aagtacccag caggaaagaa aaaacattgc acgccatttt taataaaaat atttttatta
    15601 ggtgatgttt ttttctgtac atttcatcgt atccttcagg gggctgtcac cctctcacgt
    15661 tgttgctttc atcagcaagt gcctcaataa gtttattaat acacgcccgt tctgccggaa
    15721 cctcccggcc tgtaatcggg tcaaaaacga catgatcgct ttcaacgtaa cgtaaacggt
    15781 gatagacatc tatacggagt tttgttttag cagcttcacg ttcaactcgt gttaactctt
    15841 ctttttctcg tttgctgctg aaaaacagaa accagatcat tgctatgata attactaaaa
    15901 aagggacaac ggtgcctaaa atgcttacca tttctgccat gccacccaga atagattgaa
    15961 tctttcctgt ggctgtggtg gttgttactg gttcagcaag atcagaacca gtgtctgata
    16021 aaaatgtaaa taaacgggca gataaaccaa atagcacgcc aaaaacgaga acttttatta
    16081 tcgaaccgta tacatttaat agactgtatt cttcctcttt aggcgattca aagttttttg
    16141 ccctttgtgt ctgctcttgt ttagaaccag aaacacctcc gatgaataag ccaataccac
    16201 cagtacctaa accaataccc ccaccagtac cattccattt acctgttgtt gtttctttag
    16261 ctacaatagc tgcaagagaa atcccttcat ttgcgtttcg catagaattg tcctcatact
    16321 attcctgtga cacttaacac catcaataaa atatgtaatg cagtaatatt tattagcgtt
    16381 tttttgtaga cttcaataaa atctttatcc agaagtctga taataaaaat acatagtgca
    16441 ttcatcgctg ttcccgtaag cggaattaac aacgaaagag gcatgtttta ttccttgctt
    16501 tcactcttgt cttcgatggt cattacttta agagttattc cttgcatgtc tgtaacaata
    16561 actcttgttc ccgcagggta gttttcatcg tgtaacgtct caacaggcca gaatgtatca
    16621 ccaatgagta attgcccctt gccgttaaca atatcgtcac tgagcgtaaa ttcttttcct
    16681 ttaaaacgtg aatgccctgt gttcaccagg agagtatcta atttcttatg ttttttgtca
    16741 tagaagaact taattatcga cgccagtatg ctgattgacg caaagaagca tatattggct
    16801 tcttgtgata tcggaagaaa aaagcccaca atggcgcttg ataatgcacc aagactgatt
    16861 aatagtaacc atcctgttcc ggttataatt tcaagtgcaa tacacagaag aaacagtgta
    16921 aaccataaca tggctaatct ccatgagata aggacggtga ttaaaacaat attgtcaccg
    16981 tccgctatct tttttaagac aaatattgtt gagcctgttt catatcaact ttgggagtat
    17041 cctgtgatac gttttttagc agttcactga tacctgcaac cgaactcacc aggctacttg
    17101 aatcaagcgg catcataacc agtttactgt ttgatgctgt gccaattgcc tggagtgctt
    17161 cagtgtattt ctgcgcaata aagtaattaa cagactgaac atcaccttca gcgattgcat
    17221 ccgacaccag ttttgttgcg cgagcttccg cttctgcctg acgttcgcgt gcttcagact
    17281 gaagaaatgc tgattgccgt tccccttccg ctttcagtat ttgagactgt ttttcccctt
    17341 ctgccttcag aatttctgac tggcgtatac cttccgcttc aagaatccgg gcacgcttag
    17401 ttcgttccgc tttcatttgc gcgttcatcg cttcagtcag ttcttttggt ggtttaacat
    17461 cccgaatttc aatacgtgtg accttaattc cccacgggtc agtcgcataa tcgacaaccg
    17521 ttaacagttt agaattgatg ctgtcacgtt gagaaagcat atcgtcgaga ttcattccac
    17581 caacgacagt tcggatattg gtcataacaa gatttgaaat agctgatgca agattatcga
    17641 cttcataagc tgccttcgct gcatcaatga cctggacaaa acaaacagca tcgatagtta
    17701 cgtttgcatt gtctttcgaa ataacttcct gtttaggaat atccaggacg gtttccatca
    17761 tgttgatccg ttgacctatg cggtccataa acgggatcag gaagtgaaga ccgggggaaa
    17821 gcgtatgggt atatttacca aagcgttcca ctgtccaggc attaccttgt ggcacgattt
    17881 tgacggcaga tttaacgaat atcagcgcaa ctaaaattaa taagagtaac ggaatagatg
    17941 taactattgg cgtaattata gcgtcaatca tgattaatcc tctcccaatt catcactgtg
    18001 gatgtatttc tttgcaaatg aaatctgaaa tatgaacatc aacagaaaaa cactttcctg
    18061 cttctttttc ttttgagaag ttttttctgc attcttcagc ggtcataggg aagtcgctgt
    18121 aatgagtcca tttcacgggg ccaccacctg gttttttgat ggttgcgttt atttggtact
    18181 taggcattca aaaatacctc cactcgatca cctgtctttc ttgaacacat ccagcggcgc
    18241 agttctgtaa aactatcctg gatctcacaa cgggtacttt tcattgtgac agttggtata
    18301 ttgtcgtagc tggcatatgc aatctctttt tttggggtgc gcaatatgcc tttgtcaata
    18361 tcaagaatcc tgaacgcgct aattcttctt ttgtgctcgt taaatagtgt tcgtttagtt
    18421 gctgttatct gcttctgtgc ttcctggtaa atgtaggtaa gcacatcata agattgttgc
    18481 tgtgtaataa ggaaattacg gctaacatcc gttgttgtga gcgttttatt atttcttaat
    18541 ccccaataag ccacgatgat atagagtgga tatcctttca aatctgccac acattcagga
    18601 atgacaggat taatttttaa cttacgcgcc atattttctc ccggttgttt gtctgaaata
    18661 gaaacatccc cggaatacag cgtcataagc taattatgaa aagttgcagc cactcactac
    18721 aggctcttta tctgattgtt catcatcatg catagctaaa cccccttacc tggagtggag
    18781 actggcaaat ctggtgtact tctgcgttgc agcctgtacg acttgctcag ggagagagtg
    18841 agtgagatta actcaacgcg agttcaccaa ataagggggt ttaagtatgc agaatgatat
    18901 ttgcggataa agagtcgtta cctgtgtaga tggcgcggca cgtcactctg ttacgtagcc
    18961 cgttgctgac agatgccagc ggtgcttgaa atgcacgaat aataacctcc ttatgccagg
    19021 gctttaggca atgaccgctg tccggggttg ttaaagagca tgagatgaca agcgttctaa
    19081 cgggcgtcaa agagtgctca ttttccaggc aatgagcaca aaaaacgcga tgaacgagct
    19141 ttttacaatc atctggatag tattgtgtgt gcgacattta aaacctgtca atgccacaag
    19201 agcgatctaa tgatctggat aacacctaaa aaacagaata atattccgtt tgttaaatga
    19261 tgtacgatct aaaaatctag ataatactgt ttgtgtggtt tattcgatcg tgtgagtggg
    19321 tgtatgcagt caaaaaaact tttttgaata tacaaaattc tgaatttctg attttgtaag
    19381 gttttgtatg gggaacgtca gtaaagaagg ggcttcagta gctgttctga aaacgatttt
    19441 gaagctgtca gagatgtgtg ctgaatcaaa aaggaaacga gccaggcttg aacatcgttg
    19501 atcgattgcg cccatgctgc taaatttgca ggcaagtttt ccgcgatttg taaatgcaga
    19561 aaacgaggtg ctgaaatttt gttcaagaac gtaaatttca gataaagaaa acccccgtaa
    19621 tctcgtgttt tccaggcagc gaagattagc gggggctgac atcaaggcag agccttgagc
    19681 ttatttattt caggtatgct tgttacctga agtggagttt aattccatga gaaggaaata
    19741 tcaactctta cagtgcaagc taagtgcagc ttgctgtgat tagcaaacgt cactggtctg
    19801 aactatcacc ggaagagcag gtccgtttct ggcaggacta cgaagcggga attgaaagct
    19861 cattcctggt cccccaggaa aacaaaggcg gaacaacaaa acgtcgccgg ggtgaacatt
    19921 ccactaagcc aaagtgtgag aatccagcct ggttccgtcc cgacaactat aaggcgctgg
    19981 gcggacagtt gggccacgct tacaatcgtc tcgttaaaaa agacccggtc actggtcaga
    20041 acacactcag aatgcacatg tcgttgcatc cattttatgt actgaaacgt cagagtgtcg
    20101 gtcgcaaata taaattccga ccggagaaac agcggctgct tgacgcaata tgggttgtac
    20161 ttgtgagttt ttgtgatcgt ggattgcaca ctgtcggcat gtctgtctcc cgcctggcag
    20221 aagaaataag cccaaaagac agtaagggag acgtgattcc tgaaaccgca gtcacagtat
    20281 cgcgcctttc ccgtctgctg gcagagcagg tttcctttgg cacgttggga acgtcagaaa
    20341 agacgatatg ggatcgtgaa tcccgccagc gactgccgaa atacgtctgg atcaccgaaa
    20401 ctggctggaa aatgctggga gttgatctgg tgaaacttca ggagcagcaa cgtaagcgcc
    20461 ttgcagaaag tgaaatccgt ctgcaattga ttaaagaagg cgttatccgc gagggcgagg
    20521 aaatctctgt tcactcggct cgtaaacgct ggtatgcgca gcgttctctc gatgccatca
    20581 agtcccgacg taagaaagca gcggagcgca aacgtgctaa tcgcctggca aaactgccgt
    20641 atgatgaaca gcgaaacgaa attgcgcggt ttattctgaa gcgtatgccg ccggacgaag
    20701 cgtactggtg cactaaagaa agattagagc aactggtagc cagagatcta cgtcagcttg
    20761 aactggctct gacagcttcg ccaccccact agtttagctg cctgatacaa tcggcactct
    20821 cttcagcccc ttcggggctt ttcgtgttgc ctgcatttca tgaaattcct catatttgac
    20881 gaccgttttt cattttgtcc cgctctgaca gttttttgat ggccgcaata gggtatcgga
    20941 gtaaaaacgc cttcggttat ccacaaaaac ccgcaaagga ataacttcag aaagcagcaa
    21001 acatagaaca tcaaacccgc aatatattct taacccctgt tctttaatcc cctgcgttgc
    21061 ttcgccgcag ggaaaatatt tatctttgaa acaactgtgg ataattacaa aaatgccttc
    21121 gcttgcagcg gctaacgccg cgccgctcag aatattaaaa gtacctccca ccgcttcgcg
    21181 gcgggcatat ggacttctgc ctaatgaaaa acaaagcccc tccaaccctc ctacaaaaaa
    21241 acgctgcaaa tactcagcca cagcgcccaa cccatcactg aaaagcgccg ccgcccccgc
    21301 ccgaagggcg ggaacaacat cgctttcaat aatggatgtt gtaactaaga aatctcatcg
    21361 ctgtcagcct tctggctgga agtaccaaat acacgctcgt aagcggccct gacggtccgc
    21421 taacgcggag atacgccccg actgcgggta aacccttgtc gggaccactc cgaccgcgca
    21481 caaaagctat ttcatggctg aaagcgggta tggcttagca ggatggggat gggtaaggtg
    21541 aaacctatca atcagtaccg gcttacgccg ggcttcggcg gtttagctct gtgccatata
    21601 aaacaacaga gtgccgcctt acatgccgct ggcgcggcat caaaaagaaa gccccccaat
    21661 aacgggggga aacagcgttt tgatcaaaat gataatgcta gtgtgcttct tgcgtgccat
    21721 tccaggcaca aactaaattt aacgtttcgt taactacaaa gaaatccgat gagttaatca
    21781 cttgcgaaat aaaaatcata tagtaaatta ctatatgatt ttgcgttgtt tcggagaatt
    21841 aaaaaatgcc tttgattgaa tacatcaaca agttctatcg ggggaatcag gcatcgttcg
    21901 ccaggctgac cggagttcaa cctgcgcagg taacacagtg gatcaacaaa ggattcatcg
    21961 tggtgaatca cacactgtat agtccacgcc gaaagctggg aatttaattc ctggtcaata
    22021 tataatgctg ttggcgcttg aggctttctg cctcatggcg aaaaggtggt ttgtatcttg
    22081 ttgtgcggca gaaagaagaa agccccgtag ttaatttttc attaacccac gaggcgatcc
    22141 caatgcttca acaacatcag gatagcctct taccgcgcgt tacgcaagga gaagaaggcc
    22201 atgaaacagc cgaaaaacgc cctgacctgg tgtttattaa tcgtgtgctg cacattgtta
    22261 atattcacgt atctgacccg gaatcgtttg tgcgaggtcc ggctgaaaga tggaaacagg
    22321 gaggttacgg caagtctggc ttacgaatcc aacggtaagt agcaacccgg aggcgggcga
    22381 aagcccgcct ttttggggat gatgtggttc tggcattaag cgcctttgca aagggggtat
    22441 tctgtacgga tacccccttt ttccatgtat tttcaggata ttaactgctg aaaatggtat
    22501 aattagtcaa tatgacagca attattgagg cgagtatgca gccaaaaaaa acacccgtga
    22561 ttgtggtcaa aaaacgccgc gttctggtca tgccagaaaa tcctgtggtg aatgaaaagc
    22621 cgcaggaagt acaaaaatca gcagttaatg aaaacaagaa agtacagaag aaagatgctg
    22681 tagcagagaa aacacataaa aagcagcctc agccctggta tttgaaaaaa caaatcactt
    22741 ttcccccaaa atatccgaaa gaatattttg aaaagtgttt caataaagtt cgtgctgttt
    22801 ttcctgaact atggacagac gaaaaaaaga acttgcccct gaaaagcggg attctccagg
    22861 acgttgagaa atacctggcg gataacccgg atgtggatct gacgattgaa gagtggaatt
    22921 gtgcggttca ggtgatgacg ttccggtggc aatatcttca gaattgtact gtaccaggcg
    22981 caacacgtta cgacctttac gggaaacctg ctggtacagt aaaaaaagca cacgcaactt
    23041 atgcgcaact ggttctcgat gcccgtaaga aagccagcga gaagaaacaa ttaaaacgta
    23101 agggataaag ccccgaaggg ctttatcatt tcagcctggc tgactgtaga atccagtgat
    23161 tttttccgtt ttcgtccagg ccgtgttcgt aagctgcaag agccgctttc attattgctg
    23221 tttttgtctg gcctgttcgc agtgcttccc gttccagaat atcttcgtaa acgtccccaa
    23281 gccggacatt ggtcacttta gttgctcccg attttttgcg ggtagcttca ttgatgaatc
    23341 gggctgtgtc tgcattggtt tgttctgttt tttgtcctgg tattaccgga gttttcaagt
    23401 caagagccat tatatcacct ttcgtgtgtt acattagctt tactgtgtaa agttaatgat
    23461 gtaaagctat cgtattacat ccattaggga ttgatgccta actcttgcgc caaaagctct
    23521 atttgtgctt tagctgtaga aaggctggat gcgcgacgta catcatgaac gccagcgcct
    23581 tcattacagg ccgcttcaaa aatatctaaa tctgaaatac gtgtacgaag tggctgtatc
    23641 cacaccgggt tctcccggag taacttgtcg agatcaatcg ctgcttttct gtggcgtggt
    23701 tttgtcgtgt taattctcgt gaaaagtacc cagggttgca ggtctggatt gatctgttga
    23761 gctgtgcgaa ctttttctgt tacatgagtc agcgtttctg cttcgaaatc tgaagacggc
    23821 ttaacgagag taagcagaat gtcagctaca gttaaggcac tgcgaaattc cttgctgtca
    23881 tgccctggac agtcaatgat cagcacttca catatttttt tcaggcgctt aatgacttct
    23941 gatacatcgc catactcttc ataaacagga accggtaata agccattctg ctttcttttt
    24001 tcgttccagc taagaatatc attgttttta tcggtcttca gaatgatgac cgttcgtgaa
    24061 ttatttacca gataagaagc caggtttgtt gctgtggtgc tttttcctac accgccttta
    24121 tctgatgcga caagtaatgc tctccccata gaaacctcgg atatacagtg taaaggttta
    24181 tttgtcttta cattgtattc attatagtta cgttgtaaag atgtttgtgt ctttacaatg
    24241 tgattgtttt ttgtgcttta cattgtgtgt atgttacagg gtaacttttc aggatgcgat
    24301 tttgcctggt gtgttgttgc gcccgtagct cgctgacgct cacacgcgca gtacagcgcc
    24361 ccgccctgct gcaatgcacc aggacggcgt aaactcgctg gcgctcgcgc taactgcggc
    24421 attctgcggc gcaaatcagc atgatcatgg cctgttggtc tgttctggct ttgcagggcg
    24481 aagggatggc cctgtgcatc ctggtatgtt cgtcagcggc tgcgccgcgc ttcggggcga
    24541 tgtggccccg ctgctccctg gtattttctt tcgccccttc catgccctgt cgggcattaa
    24601 atgaatataa ctatatgtat ttgatggtat tctttttgaa gaatggaggg aaaaatactt
    24661 gataataggt cagttctgac ctattataaa cacatcggga ggggatggtc cccaaccacc
    24721 aaagccgctc agcggcagga gataaaaatg aaaacattaa cttttaataa cggcactgtt
    24781 tctgttggcg atgtgtttgt atcttcctgg ggatatgagc aaacgaacgt taatttctac
    24841 caggttattt ctgttcatgg caaaaaaacc gtaactgttc aggagattcg cgcttcagtt
    24901 catcggactc attcaatgag cggatataag accccattac ttaatgattt ctgtggtgag
    24961 ccattaaaac ggcgagtgcg tgattattac agtacgccag caattgaaat tgaggaattc
    25021 gaaacagcat ataaaggatc accggaagaa aaacacgagt tcacatcata ctactaagaa
    25081 aatagttgca gggggtatcc cctgcaacca tgttaaattg ttaatagtga atacaggggc
    25141 ttaatcgcct cttttttatt tggtgcgcag taaaactccg tccttcaagg cgtggagaat
    25201 gccaggcgaa tgcagcaatg taatgttgcg cccgtagctc gctgacgctc acacgcgcag
    25261 tacagcgccc cgccctgctg caatgcacca ggacggcgta aactcgctgg cgctcgcgct
    25321 aactgcggca ttctgcggcg caaatcagca tgatcatggc ctgtcagtcc gttgggcttt
    25381 gcagggcgaa gggatggccc tgtgcatcct ggtatgttcg tcagcggctg cgccgcgctt
    25441 cggggcgatg tggtcccgct gctccctggt attttctttc gccccttcta tgccctgtcg
    25501 ggcattacat gaatctaacc atctgtattt gatggtattt attttaaaga aaggaggaaa
    25561 aaacacttga taataggtca gttctgacct attataaaca catcgagagg ggatggtccc
    25621 caatcaccaa agccgctcag cggcaggagc ttaaaaatgg aaaaccacgc caaatttgtt
    25681 gctactgaaa ttcttaatca gcttggtgga aaccgcttta ttgctatgac aggcgcaaag
    25741 aactttgcct gttttgatga aaatggtgag tgtggtcttt gctttcgttt gccttcaaat
    25801 tttgcaatga agggaattaa tttggttaaa atcaaattaa ccttctctga tacatacctg
    25861 gtgacatttt cccgtgtccg tggagctaca gtaaaagaaa tatcaaaatt tgataa
//
LOCUS       DAZPLM010000038        25637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPLM010000038 DAZPLM010000000
VERSION     DAZPLM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.038"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHVC7_001349"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHVC7_001349"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR2115449.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHVC7_001350"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHVC7_001350"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR2115450.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHVC7_001351"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHVC7_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR2115451.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHVC7_001352"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHVC7_001352"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR2115452.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHVC7_001353"
     CDS             complement(5488..5685)
                     /locus_tag="ACHVC7_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR2115453.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHVC7_001354"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHVC7_001354"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR2115454.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHVC7_001355"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHVC7_001355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR2115455.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHVC7_001356"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHVC7_001356"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR2115456.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHVC7_001357"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHVC7_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR2115457.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHVC7_001358"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHVC7_001358"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR2115458.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHVC7_001359"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHVC7_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR2115459.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHVC7_001360"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHVC7_001360"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR2115460.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHVC7_001361"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHVC7_001361"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR2115461.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHVC7_001362"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHVC7_001362"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR2115462.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHVC7_001363"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHVC7_001363"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR2115463.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHVC7_001364"
     CDS             complement(17127..17180)
                     /locus_tag="ACHVC7_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115464.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="ACHVC7_001365"
     tRNA            complement(17414..17489)
                     /locus_tag="ACHVC7_001365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="ACHVC7_001366"
     tRNA            complement(17525..17600)
                     /locus_tag="ACHVC7_001366"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHVC7_001367"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHVC7_001367"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHVC7_001368"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHVC7_001368"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR2115465.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHVC7_001369"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHVC7_001369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR2115466.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHVC7_001370"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHVC7_001370"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR2115467.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHVC7_001371"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHVC7_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR2115468.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHVC7_001372"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHVC7_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR2115469.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6015 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAZPLM010000039        25284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPLM010000039 DAZPLM010000000
VERSION     DAZPLM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.039"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHVC7_001373"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHVC7_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHVC7_001374"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHVC7_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR2115470.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHVC7_001375"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHVC7_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR2115471.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHVC7_001376"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHVC7_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR2115472.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHVC7_001377"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHVC7_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR2115473.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHVC7_001378"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHVC7_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR2115474.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHVC7_001379"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHVC7_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR2115475.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHVC7_001380"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHVC7_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR2115476.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHVC7_001381"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHVC7_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR2115477.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHVC7_001382"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHVC7_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR2115478.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHVC7_001383"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHVC7_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR2115479.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHVC7_001384"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHVC7_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR2115480.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHVC7_001385"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHVC7_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR2115481.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHVC7_001386"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHVC7_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR2115482.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHVC7_001387"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHVC7_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR2115483.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHVC7_001388"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHVC7_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR2115484.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHVC7_001389"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHVC7_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR2115485.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHVC7_001390"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHVC7_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR2115486.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHVC7_001391"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHVC7_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR2115487.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHVC7_001392"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHVC7_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR2115488.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHVC7_001393"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHVC7_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR2115489.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHVC7_001394"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHVC7_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR2115490.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHVC7_001395"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHVC7_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR2115491.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHVC7_001396"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHVC7_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR2115492.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHVC7_001397"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHVC7_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR2115493.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHVC7_001398"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHVC7_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR2115494.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHVC7_001399"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHVC7_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR2115495.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHVC7_001400"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHVC7_001400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR2115496.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHVC7_001401"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHVC7_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR2115497.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHVC7_001402"
     CDS             14267..14635
                     /locus_tag="ACHVC7_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR2115498.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHVC7_001403"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHVC7_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR2115499.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHVC7_001404"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHVC7_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR2115500.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHVC7_001405"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHVC7_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR2115501.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHVC7_001406"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHVC7_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR2115502.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHVC7_001407"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHVC7_001407"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR2115503.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHVC7_001408"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHVC7_001408"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR2115504.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHVC7_001409"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHVC7_001409"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR2115505.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHVC7_001410"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHVC7_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR2115506.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHVC7_001411"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHVC7_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR2115507.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHVC7_001412"
     CDS             21814..22356
                     /locus_tag="ACHVC7_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR2115508.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHVC7_001413"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHVC7_001413"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR2115509.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHVC7_001414"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHVC7_001414"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR2115510.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHVC7_001415"
     CDS             23753..24010
                     /locus_tag="ACHVC7_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR2115511.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHVC7_001416"
     CDS             complement(23986..24540)
                     /locus_tag="ACHVC7_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR2115512.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="ACHVC7_001417"
     rRNA            25013..>25284
                     /locus_tag="ACHVC7_001417"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5992 c         6617 g         6233 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga tatcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAZPLM010000040        25106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPLM010000040 DAZPLM010000000
VERSION     DAZPLM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.040"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHVC7_001418"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHVC7_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR2115513.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHVC7_001419"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHVC7_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR2115514.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHVC7_001420"
     CDS             1145..1309
                     /locus_tag="ACHVC7_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR2115515.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHVC7_001421"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHVC7_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHVC7_001422"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHVC7_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR2115516.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHVC7_001423"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHVC7_001423"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR2115517.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHVC7_001424"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHVC7_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHVC7_001425"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHVC7_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR2115518.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHVC7_001426"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHVC7_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR2115519.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHVC7_001427"
     CDS             14459..15955
                     /locus_tag="ACHVC7_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR2115520.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHVC7_001428"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHVC7_001428"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR2115521.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHVC7_001429"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHVC7_001429"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR2115522.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHVC7_001430"
     CDS             18049..20109
                     /locus_tag="ACHVC7_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR2115523.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHVC7_001431"
     CDS             complement(20291..21613)
                     /locus_tag="ACHVC7_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2115524.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHVC7_001432"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHVC7_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR2115525.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHVC7_001433"
     CDS             complement(23086..23985)
                     /locus_tag="ACHVC7_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2115526.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHVC7_001434"
     CDS             24505..>25106
                     /locus_tag="ACHVC7_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR2115527.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6366 c         6992 g         5854 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctgaacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggcg acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZPLM010000041        24995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPLM010000041 DAZPLM010000000
VERSION     DAZPLM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.041"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHVC7_001435"
     CDS             <1..807
                     /locus_tag="ACHVC7_001435"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR2115528.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="ACHVC7_001436"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="ACHVC7_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR2115529.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="ACHVC7_001437"
     CDS             2629..3948
                     /locus_tag="ACHVC7_001437"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR2115530.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="ACHVC7_001438"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="ACHVC7_001438"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR2115531.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="ACHVC7_001439"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="ACHVC7_001439"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR2115532.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHVC7_001440"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHVC7_001440"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR2115533.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="ACHVC7_001441"
     CDS             8980..10530
                     /locus_tag="ACHVC7_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2115534.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="ACHVC7_001442"
     CDS             10532..11554
                     /locus_tag="ACHVC7_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2115535.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHVC7_001443"
     CDS             11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHVC7_001443"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR2115536.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12444..13430
                     /locus_tag="ACHVC7_001444"
     CDS             12444..13430
                     /locus_tag="ACHVC7_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2115537.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13459..14355
                     /locus_tag="ACHVC7_001445"
     CDS             13459..14355
                     /locus_tag="ACHVC7_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR2115538.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHVC7_001446"
     CDS             complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHVC7_001446"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR2115539.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15187..15402
                     /gene="bdm"
                     /locus_tag="ACHVC7_001447"
     CDS             15187..15402
                     /gene="bdm"
                     /locus_tag="ACHVC7_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR2115540.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15504..15641
                     /gene="sra"
                     /locus_tag="ACHVC7_001448"
     CDS             15504..15641
                     /gene="sra"
                     /locus_tag="ACHVC7_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR2115541.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15798..17495
                     /gene="maeA"
                     /locus_tag="ACHVC7_001449"
     CDS             15798..17495
                     /gene="maeA"
                     /locus_tag="ACHVC7_001449"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR2115542.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17629..18631
                     /gene="adhP"
                     /locus_tag="ACHVC7_001450"
                     /pseudo
     CDS             17629..18631
                     /gene="adhP"
                     /locus_tag="ACHVC7_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18777..19061
                     /locus_tag="ACHVC7_001451"
     CDS             18777..19061
                     /locus_tag="ACHVC7_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR2115543.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHVC7_001452"
     CDS             complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHVC7_001452"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR2115544.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHVC7_001453"
     CDS             complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHVC7_001453"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR2115545.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHVC7_001454"
     CDS             complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHVC7_001454"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23292..23294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR2115546.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24111..24992
                     /gene="yddG"
                     /locus_tag="ACHVC7_001455"
     CDS             24111..24992
                     /gene="yddG"
                     /locus_tag="ACHVC7_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR2115547.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6098 a         6364 c         6346 g         6187 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 actgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
    11701 ccgtggcttg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
    11761 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
    11821 gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt
    11881 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
    11941 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
    12001 gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg aattcccttt
    12061 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
    12121 gcgaaaacct ttggtgcgtc gcgttggcat ctgatcagct ggcatatttt acgtaactcc
    12181 ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
    12241 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
    12301 gctaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
    12361 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
    12421 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
    12481 tgagtttccc cggttttaac ggttatgttc acgtgctcaa caatgtgtcc ttgcagatta
    12541 accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
    12601 tgctgattat gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac
    12661 tgctgggaga cgatgttctt aacgctcggg agaaacagct tcgtcagtgg cgcggcgcac
    12721 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc
    12781 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
    12841 aagcgattac cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
    12901 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
    12961 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt
    13021 tgcaggtact gcgtttgctt aaacataaag ctcgcgccag tggaactgcg gtactgttca
    13081 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
    13141 gaagcgtaat tgaaagcggc gtgacggcag acgttatcca tcatccccgg catccgtata
    13201 ccattggttt gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca
    13261 ttccagggac ggtaccaaac ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt
    13321 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
    13381 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtatctga cacgttatta
    13441 acgttacgcg acgtccatat caatttcccg gcccgtaaaa gctggcttgg taaaactacg
    13501 gaacatgttc atgcgattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
    13561 atcgtcggtg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg
    13621 caaccgagcc acgggcaata catccgttca ggatcacaac gcattatgca gatggtgttt
    13681 caggacccgc tctcttcgct taatccgcgc ttatcggtgt ggcgcatcat cacagaaccg
    13741 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaaa ggagttggct
    13801 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
    13861 cagcggcaac gcatcgccat tgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13921 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
    13981 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    14041 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14101 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14161 tccctccccg ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg
    14221 ccgggaaacc gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc
    14281 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14341 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14401 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14461 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14521 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt
    14581 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14641 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14701 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14761 cgccactctg ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14821 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14881 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    14941 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat
    15001 attgcatcaa tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa
    15061 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15121 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15181 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15241 gccattgagc aaggccttcg ggcagagcaa ggtgttgtca ctgaagatga cattctcatg
    15301 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15361 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15421 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15481 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15541 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15601 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15661 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15721 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15781 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15841 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15901 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15961 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    16021 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16081 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16141 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    16201 tggacgatat tctgcaaaac gtaccgaacc ataatatcaa agtgattgtg gtgactgacg
    16261 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16321 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16381 tggatgtcgg aacgaacaac caacaactgc ttaacgatcc gctgtatatg ggctggcgta
    16441 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    16501 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16561 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16621 cggcggtaac agtcggcaca ctgatcgcag caagccgagc ggcaggtggt cagttaagcg
    16681 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16741 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16801 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
    16861 tggtgcagaa acgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16921 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16981 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    17041 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17101 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17161 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17221 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17281 cacagtattc gccgctggtg ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    17341 ttcagaaagt ctcccgtgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17401 tggcggtgaa aacctctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    17461 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17521 cgggcaattt tcctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17581 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17641 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    17701 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17761 aatggcgatt ttggtgacaa aaccgacgta attctgggcc atgaaggcat cggtgtggtg
    17821 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17881 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17941 tcagttaaaa atgccggata cagcgttgat ggcggaagag tgcatcgtgg tcgccgatta
    18001 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    18061 ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat
    18121 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
    18181 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc
    18241 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
    18301 tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
    18361 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
    18421 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
    18481 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    18541 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    18601 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
    18661 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
    18721 ggaacaataa tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga
    18781 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
    18841 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
    18901 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
    18961 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
    19021 cggtggatgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccttataaag
    19081 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
    19141 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
    19201 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
    19261 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
    19321 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
    19381 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
    19441 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
    19501 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
    19561 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
    19621 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
    19681 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
    19741 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
    19801 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
    19861 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
    19921 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
    19981 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
    20041 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
    20101 agaccgtaat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
    20161 ccgacccctt tcgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
    20221 acgcgctctt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
    20281 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
    20341 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
    20401 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
    20461 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata
    20521 cagccgtctt tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc
    20581 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
    20641 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
    20701 atacaggtgg aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa
    20761 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
    20821 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
    20881 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
    20941 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
    21001 agcggtttca gacggcgcgt taccaccgcc accgcgcgga taaagccacg cttgcttgag
    21061 acagtgacac gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc
    21121 acaaactgtt ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag
    21181 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
    21241 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
    21301 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
    21361 ttgttgatgg caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc
    21421 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
    21481 cactggatca gcatccgttt cggatcccac ggtttaccat tgatatccgc cgaagcacgg
    21541 ttgtagagca cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
    21601 ccggatgggt cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg
    21661 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
    21721 agcaactgac ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca
    21781 tagccgttat tctctttcgc cacttcgtca gattgcggtt cgtgcggctg cttgtagttc
    21841 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc
    21901 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
    21961 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttcg
    22021 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
    22081 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
    22141 accatgtact tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca
    22201 accgggttaa agccctggca gaagtaaccg gtgactttgc cttcgtccat catgttgaaa
    22261 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
    22321 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
    22381 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
    22441 gactgcaaat caacctgttt ttctgacgga agcgtcagat aacctggcag gctggtagag
    22501 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
    22561 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
    22621 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
    22681 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg
    22741 cagatgtttt caacgacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
    22801 acacagcgcg gatgagtcag tgtttcatcg cgttttgcat agccgttttc atcgaactga
    22861 tagttccagg acgacttatc atactggcgt ttttcagcgt cgtagccgct gaacaggccg
    22921 tcttcgaaag caaaatcatc acgcaccagc aggctggcgt tggtgtaatg tttaacgtat
    22981 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
    23041 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga
    23101 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
    23161 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
    23221 atatccaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
    23281 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
    23341 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
    23401 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
    23461 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
    23521 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa
    23581 tccagcaaac cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca
    23641 ccttcaatgt gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc
    23701 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
    23761 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
    23821 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
    23881 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
    23941 tttttgcgcg aaatcaaaaa gaacacgtgt atgccacaaa ataatccgcc tgcgcaaatt
    24001 aacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
    24061 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
    24121 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
    24181 ttcgcggtgt cagcgagggg ctcggtccgg tcggcggcgc agctgctatc tattcattaa
    24241 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
    24301 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
    24361 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
    24421 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
    24481 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
    24541 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
    24601 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
    24661 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
    24721 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
    24781 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
    24841 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
    24901 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
    24961 tgctctgctg gctggcgaca cgtcgtggtt aaaat
//
LOCUS       DAZPLM010000042        24430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPLM010000042 DAZPLM010000000
VERSION     DAZPLM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.042"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            48..764
                     /locus_tag="ACHVC7_001456"
     CDS             48..764
                     /locus_tag="ACHVC7_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR2115548.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            794..1294
                     /locus_tag="ACHVC7_001457"
     CDS             794..1294
                     /locus_tag="ACHVC7_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR2115549.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1386..4070
                     /locus_tag="ACHVC7_001458"
     CDS             1386..4070
                     /locus_tag="ACHVC7_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR2115550.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4067..5155
                     /locus_tag="ACHVC7_001459"
     CDS             4067..5155
                     /locus_tag="ACHVC7_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR2115551.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5238..6535)
                     /gene="exuT"
                     /locus_tag="ACHVC7_001460"
                     /pseudo
     CDS             complement(5238..6535)
                     /gene="exuT"
                     /locus_tag="ACHVC7_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            7018..8430
                     /gene="uxaC"
                     /locus_tag="ACHVC7_001461"
     CDS             7018..8430
                     /gene="uxaC"
                     /locus_tag="ACHVC7_001461"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR2115552.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8445..9932
                     /gene="uxaA"
                     /locus_tag="ACHVC7_001462"
     CDS             8445..9932
                     /gene="uxaA"
                     /locus_tag="ACHVC7_001462"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR2115553.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10015..10566
                     /locus_tag="ACHVC7_001463"
     CDS             10015..10566
                     /locus_tag="ACHVC7_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR2115554.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10571..11815)
                     /gene="sstT"
                     /locus_tag="ACHVC7_001464"
     CDS             complement(10571..11815)
                     /gene="sstT"
                     /locus_tag="ACHVC7_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR2115555.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12431..13396)
                     /gene="alx"
                     /locus_tag="ACHVC7_001465"
     CDS             complement(12431..13396)
                     /gene="alx"
                     /locus_tag="ACHVC7_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR2115556.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13679..14665)
                     /locus_tag="ACHVC7_001466"
     CDS             complement(13679..14665)
                     /locus_tag="ACHVC7_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2115557.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14744..15427)
                     /locus_tag="ACHVC7_001467"
     CDS             complement(14744..15427)
                     /locus_tag="ACHVC7_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR2115558.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15504..16007)
                     /locus_tag="ACHVC7_001468"
     CDS             complement(15504..16007)
                     /locus_tag="ACHVC7_001468"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR2115559.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16092..17228
                     /gene="rlmG"
                     /locus_tag="ACHVC7_001469"
     CDS             16092..17228
                     /gene="rlmG"
                     /locus_tag="ACHVC7_001469"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR2115560.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17513..17827
                     /gene="higB"
                     /locus_tag="ACHVC7_001470"
     CDS             17513..17827
                     /gene="higB"
                     /locus_tag="ACHVC7_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR2115561.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17824..18240
                     /gene="higA"
                     /locus_tag="ACHVC7_001471"
     CDS             17824..18240
                     /gene="higA"
                     /locus_tag="ACHVC7_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR2115562.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18285..20303)
                     /gene="fadH"
                     /locus_tag="ACHVC7_001472"
     CDS             complement(18285..20303)
                     /gene="fadH"
                     /locus_tag="ACHVC7_001472"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR2115563.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20529..22880)
                     /gene="ygjK"
                     /locus_tag="ACHVC7_001473"
     CDS             complement(20529..22880)
                     /gene="ygjK"
                     /locus_tag="ACHVC7_001473"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR2115564.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22897..23974)
                     /gene="ygjJ"
                     /locus_tag="ACHVC7_001474"
                     /pseudo
     CDS             complement(22897..23974)
                     /gene="ygjJ"
                     /locus_tag="ACHVC7_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24108..>24430)
                     /locus_tag="ACHVC7_001475"
     CDS             complement(24108..>24430)
                     /locus_tag="ACHVC7_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115565.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6175 a         6200 c         6256 g         5799 t
ORIGIN      
        1 tctaatacac caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa
       61 ttaatctctt atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt
      121 caaatatgac tgtttatcct atggctgttt ccattaatag tcagggtgaa ggcaacgttc
      181 gcgtaatatc taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata
      241 atccatcgac gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg
      301 ttgttatgcc acctaaattc gctttaccag ccggtagtag caaaactgtg cgctttgttg
      361 caatggaacc agagcaaaaa gaaaaaaatt atcgcgttaa atttgaagcg gttcccagta
      421 ttgatgacgt tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt
      481 gggggattgt tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg
      541 cccaaaaatt agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta
      601 aaaataatag caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccttggtc
      661 aggaaagaaa tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact
      721 ggatcaccaa agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa
      781 cgaaggaata gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc
      841 tgttgcaggc tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc
      901 cgctctggat atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata
      961 tctgccgggg cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt
     1021 taccaaagat gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc
     1081 tagcaagatt gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc
     1141 aaccactttt accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc
     1201 taaaaacctg atgtttgctc aaaccaccaa aggtgttctg gagacaggca tctaccgtgg
     1261 tgtagtaagc atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac
     1321 aaagggaggg gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga
     1381 ttgccatgga taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg
     1441 cgacattaac cgaaattccc gcagggtttg aggttattgc tcagggacag caggagtata
     1501 tcgaggttta tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata
     1561 ccgtaacatt tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc
     1621 agaaaatcgc gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat
     1681 tggcttgcgg ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg
     1741 aaataatcta taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt
     1801 cagctttcga tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta
     1861 tacatcagca agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg
     1921 gaaacggtgc gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg
     1981 gttatgatgc agatgatgtc agtgacagta atgctgacgt cagcgatctc tattatcgtt
     2041 atgatttttt acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta
     2101 atgcacaagg cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc
     2161 gtatcgggtc gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa
     2221 tggttctgct ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat
     2281 cgttttatct caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct
     2341 acagcgtggc attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt
     2401 ttaccaaaac cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa
     2461 ctacatcaca ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat
     2521 tacatccgca atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg
     2581 agttaggtaa taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca
     2641 gcgtgttccg taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc
     2701 cgggatggcc gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg
     2761 acagcagaga gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac
     2821 agaattttgt cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg
     2881 atagtttgcg ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag
     2941 cgcaaagtat ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact
     3001 ggattttgag tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg
     3061 acaacggctt gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca
     3121 acagccattc aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa
     3181 cgtcatggca gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg
     3241 taaccgtcgg aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg
     3301 ggcaatacgg aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc
     3361 atctttcggc ctttacgggc acttatagct ctacgctggc tgtcagttgc tatggcgtta
     3421 atttgggagc cagtggtaca gacgatttgc tgggtgcggt actggtggat gtgaaaggct
     3481 tctctgaaca ggatgaagag agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc
     3541 gaactttgca gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg
     3601 gttttgttga ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta
     3661 acggtgcagg gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt
     3721 ctgccagctt taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat
     3781 tcccgattgt ggggctgaac agcgccatgt tactggtagc tgaagatgga ggatttacac
     3841 ttgaaattaa cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt
     3901 gtccactgag tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta
     3961 gtgtggtgac ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc
     4021 ctaaattacg tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt
     4081 gattgcggcg atattgggct tgtttggcac gctcattggc gttcaggcag ctcctgacgt
     4141 gaccagtgaa attacgtatg atttggcatc tggtagagcg gattattact tctggaagga
     4201 tgaagcgtcg gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca
     4261 acaaacctgt acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg
     4321 cagtgggatg cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga
     4381 tgaagcgccg ccaggatgca aggggggcaa agggcttcag acgaatctta aggattctaa
     4441 tagatcttca tgtacagaag atggtcaaca ttattatata tacgatacaa agtttcttac
     4501 gctctacctt gagcagacag agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa
     4561 agttaaatta cattcgaaca gcccggccca ggactatttc gcaaatatta ccctgaatac
     4621 gctcgacccc aaccatattg acgtgttctt cccggagttc gcccacgcca cgccaagggt
     4681 gcagttagac ttgcatccaa caggaagcgt taacggcagc aactacgcgc aagatctgac
     4741 catgttggac atgtgcctgt acgatggttt taacggtaat gccatcagtt atgaaatcat
     4801 gctcaaagat gaagggcgac cagctgcagg gcgcagagac ggtgacttct ctatctatcg
     4861 tcaaggaggg accaccaccg acgagggaga acgcattgat taccgggtca aaatgtacaa
     4921 cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct ggaacagcat
     4981 taacctgaaa cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg tgatgtgtgt
     5041 gccgacaccc ttgactctgg cagtcgataa attcagcgtg atggataaac aggccgggta
     5101 ctacatgggc aaattgtcag taatctttac gccttccttg ccaaccatca attgaccccg
     5161 cagaccagaa aaaactaaac aacgaagccg cctgataagg cggcttcgtc attacaactt
     5221 acgttaaacg ttaaacgtta atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc
     5281 tcaatcgccg gtttgttctg caatacggtc cagataacca gcgcacccag caggtcaaat
     5341 actgccagaa ctgcgaacag cgggctgaag ccgatggtgt cagccagtgc accgacaacc
     5401 agcgcaaaca gcgtacttgc cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc
     5461 acttcgttac gaccgaacac gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg
     5521 tgggcaaaac caccgataca cagcagcata attgcgacat acgggttggt gaacagaccg
     5581 atcatacccg ggccaatcat cagcacagca cccagcgtta cgaccatctt acgggaaacg
     5641 atcaggttca caccaaacca acgctggaac agcggcggca ggtaaccacc gaggatacaa
     5701 ccgaggtcag caaacagcat cggcatccag gcgaacatcg cgatctcttt caggttaaag
     5761 ccgtaaactt taaacatgaa cagcgggatc cacgcgttaa aagtacccca ggccggttct
     5821 gccaggaaac gcggcagtgc gataccccag aactgacggt tacgcaggat ctgaccaacg
     5881 gacattttct tcgccgtgct cacctggtgc tgggcttcct gaccattaat aatatagtcg
     5941 cgttcttcat cggtcagatg cttctggtcg cgcggatgtt tatagaaaat cagccatgcc
     6001 atcgcccaga taaagctcaa tgcaccggag atgataaatg ccatctgcca gctgtgcatc
     6061 acgattgcca taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa
     6121 agtagcctac tgcgatggaa cgctctttcg ccgggaacca ttcggagctg gctttcagac
     6181 ccgccgggat catcgctgct tccgcggcac cgaccgcacc acgagcaaca gccaggccac
     6241 cccagctacc tgccagcgcg gttacaccac agaacacggc ccacagcaca gcaaacattg
     6301 cataaccgat tttcgtaccc agcacatcca gtacataacc tgctaccggt tgcatgaccg
     6361 tataagcagc agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact
     6421 cttccatcag agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg
     6481 tgccgagcgt caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa
     6541 acctcatcgt tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga
     6601 tgttagttac gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     6661 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     6721 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     6781 ctttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     6841 agcgaggcca gtactggcgc ggcctgcagc gagatttatc actttgagag taattttttt
     6901 aactacgttt attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata
     6961 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     7021 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     7081 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     7141 gcggaagact atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa
     7201 tggcgcgcta tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcatctgac
     7261 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     7321 cactggacgc accttgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg
     7381 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     7441 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     7501 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     7561 ccgagctggc gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg
     7621 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     7681 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     7741 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     7801 ctggctggcg aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     7861 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     7921 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     7981 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     8041 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     8101 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     8161 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     8221 ctgctgagtc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     8281 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     8341 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     8401 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     8461 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     8521 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     8581 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     8641 tggcggatat tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg
     8701 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     8761 gtgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga
     8821 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     8881 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct
     8941 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     9001 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     9061 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     9121 agcaggatga tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc
     9181 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     9241 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     9301 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     9361 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     9421 acgactttaa acagtacttt attgcccatg atcagccgat ctacgaaaac ccatcaccgg
     9481 ggaacaaagc gggcggtatc accacgctgg aagacaaatc acttggctgt acccagaaag
     9541 cgggttccag tgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgcctgggc
     9601 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     9661 gctgccatat ggtgctgttc agtactggtc gcggcacgcc atacggtgga tttgtgccga
     9721 cggtgaaaat cgccaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     9781 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     9841 ccatcgttga gtttgccaac ggtaagcaaa cttgcaacga gcgtaacgac ttccgcgaac
     9901 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     9961 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc
    10021 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
    10081 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
    10141 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
    10201 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
    10261 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
    10321 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
    10381 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcaca
    10441 atgattgagg gtagttttct gcttatgaat ggcttgaata tcattcgttt ctggcggatg
    10501 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg
    10561 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
    10621 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
    10681 atacgccgat gataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
    10741 acatattaca ggccagcggg atcagcagca gagaaccccc cgccacgccg gatgcgccac
    10801 aggcacacag agaagccacc acgctcaata gcagcgccgt gggcagatcg accgggatac
    10861 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
    10921 tgatggtggc acccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt
    10981 cacacagtgc catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca
    11041 cgccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
    11101 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
    11161 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
    11221 gaccaaaaat accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat
    11281 tcgacatatc gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga
    11341 ggccaattgc ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt
    11401 tggaaaccat gctcattacc agcccgcgca tcacttctac aatgcctgac ggtggcgaaa
    11461 tatcacccgc gctactggat aagtgcaggg tagaagggaa ggcgaagctg aaaacgactg
    11521 cggccagagc agcagagaag gtacccagca gatagaggaa caaaataggg cggatattgg
    11581 ttttctgccc gtgctggtgg ttagcaatag atgccatcac cagcatcaac accaggatgg
    11641 gggcaacggc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
    11701 cgggttttga tatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
    11761 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
    11821 gatcctttca ttgtgttatc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
    11881 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
    11941 gctggatttt aaagagaagt catattttga aacttaatgg ttacatttgt ttgacataaa
    12001 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
    12061 tatgagaacg gttgcgctcc tttaatgtgc tatgccatat aaagtcactg gcgcagggag
    12121 cgcgcagggg gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat
    12181 cgccgctgcg cgattccctc agcttatccc ttgcggctac cgggtcgggc gcaagggaac
    12241 gtccctgtaa gatgcgccct cgccgcgcat ccatgcgcgt cgccccggcc tccagggaac
    12301 gcttcggcga ttttgacgcc accaacaccc gtgcggtctt tttgtaaaga gctacaagcc
    12361 gattacataa ttaatagctc acctttgtct ggaatgagtt atctctagga atggcttaat
    12421 atacagcgaa tcattctccc cgctgcttat catgccgata attcacccag gcgttgataa
    12481 taaacgtcat caccagaatg ccaaacacca cgcccagcga gacggcgatt ggaatatggt
    12541 agaagtcgac aatcagcatc ttgataccga taaacaccag aatcaccgcc aggccatatt
    12601 tgagcatcga gaaacgctct gctacgcccg ccagcaggaa atacatcgca cgcaggccga
    12661 ggatcgcaaa caggtttgag gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag
    12721 ccggaatgct atccacggcg aaaatcacgt cgctcaattc caccagaatc agtaccagca
    12781 tcagcggtgt ggcgtacagc aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg
    12841 tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg
    12901 attcatcttc atgggcgagc gccatcttca cgccggtaaa cagcaggaag gcaccgaaga
    12961 tatacaggat ccagtcgaac tgcgaaatta gccagctgcc agtgaagatc atgatggtac
    13021 gcagaacaat cgccccgagc acgccataca ccagcacgcg gcgttgtaat gccgccggaa
    13081 cagagaaata gctgaacaac atcagccaga caaagacgtt atcgaccgcc agcgatttct
    13141 caatcagata acccgtgaga aaggccagtg cctgtggatc ggcaacggcg cgaccttcag
    13201 tttgcaccag ataccaccag aaagcggcgt taaacagtaa cgacagcgtc acccagacca
    13261 gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca
    13321 acaggtcgat agccagcata atggcgacaa caacagcgaa tccgccccat agcaacggcg
    13381 tgccgacagt attcatagaa gttccttaca cataaaaaaa cggccaacgt cggaagacgt
    13441 cagccgctgc tttttatgca tagacctcgc cttccggcaa ggtctcactt acaacaaaaa
    13501 aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt ttttcacaca
    13561 tcgtaatgac gatcgaccgg caatgaagtt actccccttt gcaggtaaca aagtatgaca
    13621 gaccattcag ccagtcaatg gtctgttaca taccttttac actgttttac tttggcaatt
    13681 atagttttac gctatctgcc ggaaaaatca ccccagtctg gcggcggatc tcagtcagca
    13741 gtttggcggt gatgcgactg accgccagcc ctggatgatc caccagatgc tcatccacca
    13801 gggtagcaaa cagctctgct tcgtagagca tggtattaat atgctgcggc tgggtgagat
    13861 cctgcatctg gctgccacgc ggcacgaagc acactttctg gcattcagac agtttttcaa
    13921 tcaccagcga tcccgcttcg ccctgaatct cgctttccag gacagaatca ctgacctttg
    13981 agtgctgcaa cgtaacgctg aaatcaccgt aatccatcac caccacgccg tgggcatcta
    14041 cgccgcttgc caacaaactg gcggttgcct gcacgctttt cggttcaccg aataatgcca
    14101 ccgccgacgc caggcagtaa aagccgatat ccataattga accgttagag aatgccggat
    14161 taaaggtgtt gggattctca ccatccaggt aacgttgata ccgcgaggaa tactggcaat
    14221 agttgaaaaa gactttacgc aatttgccga ctttcggcag cgcctggcgc agcaaatgaa
    14281 agttcggcag gctggcggtt ttaaatgcct caaacagcac cacctgattt tcccgcgcac
    14341 aggcaatggc ggcatccact tccgccagat tcgacgccag tggtttctcg caaatcacat
    14401 gaattttatg gctaaggaaa agttgtgtct gggaaaagtg cagggcattc gggctggcaa
    14461 tatacaccgc gtcaatggca tcgctttccg ccatcgcttc cagcgaggta aacagatgct
    14521 cgacagaaaa atcattggcg aagtgctggg cctgttcaag gctgcgggaa tatacggcgg
    14581 ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg
    14641 taccaatcac agcgaaacgt atcataacgc gtggatactc cataaaggga ttctgtcgtc
    14701 aatgtagcac gcctttttca tcttcgacgt gcgcagatac gcattattct ttcgccggac
    14761 agccatgctc actaaacggc ccaatcatga ccgatggccc gagatatttc ggctggcgat
    14821 ggaggatttt ggcgtcaatc accgcgctga cacgagcgag tttctcccgc agttgagtat
    14881 aaaaaccgtg cttcatatta aggtctggcg cgttaaaccc gatagcgtcg atgccgtact
    14941 gttttgccag ccagatggcg cgttggttat gaaattcttg cgagataatg gtgatatggt
    15001 tttccccaaa cacctttttg gcacgcacca ctgaatcaag cgttgagaat ccggcatagt
    15061 cgcagaagat cactttcgcc ggtacgcctt tagcgatcaa agcctgctgc atacctgagg
    15121 cttcatcgta atttttacgt ccgttatcgc cactgactaa cagccatttc acttttccga
    15181 cgtggtacaa cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta cccggcctcg
    15241 cccccagcaa taaacccacg ttacgcgccg gaactgcgtt gacatcgctc caggtcagct
    15301 gcttgctggc gttgaccatt acgcgatcgg cgataaaaat ggtcgctcct gccacaagta
    15361 acagtaagca ggtaatcttt agagtccggc gagaaaaaca aatacggaga agaaggcggg
    15421 gctgcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga
    15481 aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc
    15541 cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca gcacaactgg
    15601 taaaacgctt tgttatgctc tttctccttc aggtgcgcca gttcgtgcac cacgatcatg
    15661 cgtaaaaacg gttccggcgc gttgcgaaac acagtagcga cgcggatctc tgcttttgct
    15721 ttcagcttgc cgccctgcac acgagaaaca gcggtatgta atccgagcgc gtttttcagt
    15781 acgtggatct tattgtcata catcacttta gtgatcggcg gggcattgcg cagaaactga
    15841 ttttttagat cctgggtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc
    15901 ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg
    15961 gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa
    16021 aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt
    16081 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    16141 cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc
    16201 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    16261 cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg
    16321 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    16381 tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa
    16441 cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    16501 gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggag ctgttcgaga
    16561 aagtgctcgg cccgaccacc actacgctgg catggaaaaa agcgcgcctg attaattgca
    16621 ctttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    16681 ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctg gatattggcg
    16741 cacgcttctt tatgcaacat ctgccagaga atctcgaagg tgagattgtc gatctcggtt
    16801 gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa ccctcaggcg aaagtggtgt
    16861 ttgtcgatga atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc
    16921 cagaggcgtt agatcgcagc gagtttatga tcaacaacgc gctctccggc gtggagcctt
    16981 tccgctttaa tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata
    17041 acgtcgcctg ggagatgttc caccatgccc gccgctgcct gaaaatcaac ggcgagctgt
    17101 atattgttgc caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact
    17161 gcaccaccat cgccacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc
    17221 gtcggtaaga ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt
    17281 cagcacgtta ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc
    17341 cagttctgga atcgcgcttc cataaaacaa aaaatccctc tcaccatcct ctacattctc
    17401 ttgtttagcg tttttctacg tttattcttc cgtcacacag ataaattcat ccgttgccaa
    17461 tctgtcaacg gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct
    17521 gataactcaa aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt
    17581 ggtggctctg gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc
    17641 agtattccca tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg
    17701 gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg
    17761 tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa
    17821 aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc ggtggcacct
    17881 tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat
    17941 ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgccaa aataaccgcg
    18001 tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc
    18061 ggtatagccg tgattcgtac ccttatggat caatatggtt taacccttgc cgatctgccg
    18121 gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg
    18181 gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa
    18241 cgcgacgtgc cggatgcgtt gcatccggcg cttaaaataa cgatttaaat ctccagcgcc
    18301 agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac atcgcagccg
    18361 ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc
    18421 tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc gccgttgatc
    18481 accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat cattttcaca
    18541 ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc
    18601 ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt
    18661 ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag
    18721 aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca
    18781 aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc
    18841 agtacattga gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc
    18901 ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg
    18961 gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta atagcgcagc
    19021 gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg cccgccgatc
    19081 tcgctgtggg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac
    19141 gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac ggcaggaagg
    19201 attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca
    19261 acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct
    19321 cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg tgccatcgat
    19381 accatatcgg catcgccgcg cgagagaata tcgtcggcaa cctgcggatc gttaatccgg
    19441 ttggtggtta ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac ccagctaaat
    19501 gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca gccaatgccg
    19561 gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagctctac cgtttcggca
    19621 aaagtcccgc cgtcttcgac caggtcgagc atcgacagtc ggtagataat aatgaagtcg
    19681 ttgccgacgc gttcgcgcac cgcacgcact acttctacgg caaatcgcat ccggttgcgg
    19741 taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa ttcgttgatc
    19801 aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg tgccagttgc
    19861 gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga
    19921 acgaaacggt tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg ttggtagctg
    19981 tagcgcccgg tatgcaaaat ttgcagggct attttgccgc cttcctgatg taccgcttcg
    20041 gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc accttccatg
    20101 ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac gccgtgacgg
    20161 gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgt ccgggtattc ctccagcccg
    20221 gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcggg
    20281 gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc
    20341 ggatgagttc taatttagcc gtcgatggag aaaagggaaa aggggagtgc ggggtttgtg
    20401 atgggattca aaaagaatac gcccggtggt aacgccggat gcaacgcttg ccgcgtttta
    20461 tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagcc
    20521 gttgcaaatt actgcttacg gaaaaaatcg ttatacaaca tatacaaatg agcggcactc
    20581 caggagaaat ttggtgcgcc ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt
    20641 gggccatcgg cggttaatcc tttggcgtgc cggaagaacg tatccgccag cttcagggca
    20701 tcatcacgat aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat
    20761 acgcgcccac gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc
    20821 agcgggacaa aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggcg
    20881 ttggcctgcg ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc
    20941 tcaacaatcg gtttgcccgc gcagccgttc gccagtggtt tatcttcaat acgtacgtca
    21001 tagtagaact gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt
    21061 gccaactggc gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc
    21121 agataatggt tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag
    21181 tagcccagca acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta
    21241 cgtttgccgc cattagcgac atatttatcc agctgttctt tgtcgataaa cccaaagacg
    21301 gcggcatcat cacgccccga ttcccacgat gcagcaacct gtgccggaat ttccagactg
    21361 tcgtacaggc ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct
    21421 ttatcgcctt ttttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg
    21481 cgggtcgcgc catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag
    21541 tcgtgatagg ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg
    21601 ttgtacactt ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag
    21661 ttgccgccat cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag
    21721 cccacatcct gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg
    21781 atattctctt tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta
    21841 tcccacggcc aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg
    21901 ttaaatttca ccgcaccgcc cggtgagagc cagttaccgt tgagcgtttc gatcgctttc
    21961 accgcgacgc gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcaggtat
    22021 tcttcccagc gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg
    22081 atctgcattt gctctttgct aacttcctga gcggtcagca gatgggaata ggtggtgtag
    22141 agcgtggtcg aaccgttgat atgcgcctta ctggtaaagc gattaccgtt gatttcagtc
    22201 tgcaccggca gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag
    22261 gtggcgcgca ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc
    22321 tgatagtcag ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct
    22381 tccagttttt ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt
    22441 ttggtttcca gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca
    22501 tcttttgctg tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag
    22561 tcgactttct tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg
    22621 atgtactctt ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtca
    22681 ggcaacagat gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg
    22741 tcgtaatcgt aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa
    22801 ttgtcggcgt tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt
    22861 aaaatagttt ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag
    22921 gaagatttgg tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc
    22981 cgtggcgaaa cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt
    23041 ttcatcgtca gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac
    23101 gccctttaac gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc
    23161 cagcccgtcg gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag
    23221 gcccgtgaga tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat
    23281 gccttcggca aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc
    23341 gtcatagcga tcgtatttgc caaaatgtag ccaggctttg ctttcatcat gatgtccggc
    23401 aggagtcgcg gtgacgctat aacgaaagtt gccgcttaag ttctgtacct tcagcgcagt
    23461 cataatgtcg cgggtattgg gaatgacgta gcccagatcg ggggtgaaat cgccccacca
    23521 ttgcaggtca tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt
    23581 ggtacgataa ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc
    23641 tggcacccag gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag
    23701 gccgaaccag gagccgtact tcatctccag gccaccttca atataggtac cgtcgctgta
    23761 tttatgttta ttctcaccct ctaaatccat atatccacca ccccctaatc cctaattcgc
    23821 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    23881 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    23941 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    24001 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    24061 ctcatccagg aagagggaaa atgcctgctc attttttatt attctgctta atcaacattg
    24121 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttactg
    24181 tatttccacc acgcaaagcc aaggaagata acaataccgc cgacgttata aaaaatgatg
    24241 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    24301 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    24361 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    24421 atggacgctg
//
LOCUS       DAZPLM010000043        23865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPLM010000043 DAZPLM010000000
VERSION     DAZPLM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.043"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(91..1344)
                     /gene="amiC"
                     /locus_tag="ACHVC7_001476"
     CDS             complement(91..1344)
                     /gene="amiC"
                     /locus_tag="ACHVC7_001476"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR2115566.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1576..2907
                     /gene="argA"
                     /locus_tag="ACHVC7_001477"
     CDS             1576..2907
                     /gene="argA"
                     /locus_tag="ACHVC7_001477"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR2115567.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2969..4795)
                     /gene="recD"
                     /locus_tag="ACHVC7_001478"
     CDS             complement(2969..4795)
                     /gene="recD"
                     /locus_tag="ACHVC7_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR2115568.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4795..8337)
                     /gene="recB"
                     /locus_tag="ACHVC7_001479"
     CDS             complement(4795..8337)
                     /gene="recB"
                     /locus_tag="ACHVC7_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR2115569.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8330..11218)
                     /gene="ptrA"
                     /locus_tag="ACHVC7_001480"
     CDS             complement(8330..11218)
                     /gene="ptrA"
                     /locus_tag="ACHVC7_001480"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR2115570.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11394..14762)
                     /gene="recC"
                     /locus_tag="ACHVC7_001481"
     CDS             complement(11394..14762)
                     /gene="recC"
                     /locus_tag="ACHVC7_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR2115571.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14775..15098)
                     /locus_tag="ACHVC7_001482"
     CDS             complement(14775..15098)
                     /locus_tag="ACHVC7_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR2115572.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15083..15490)
                     /locus_tag="ACHVC7_001483"
     CDS             complement(15083..15490)
                     /locus_tag="ACHVC7_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR2115573.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15487..16050)
                     /locus_tag="ACHVC7_001484"
     CDS             complement(15487..16050)
                     /locus_tag="ACHVC7_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR2115574.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16041..16511)
                     /locus_tag="ACHVC7_001485"
                     /pseudo
     CDS             complement(16041..16511)
                     /locus_tag="ACHVC7_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16696..17490)
                     /gene="thyA"
                     /locus_tag="ACHVC7_001486"
     CDS             complement(16696..17490)
                     /gene="thyA"
                     /locus_tag="ACHVC7_001486"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR2115575.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17497..18372)
                     /gene="lgt"
                     /locus_tag="ACHVC7_001487"
     CDS             complement(17497..18372)
                     /gene="lgt"
                     /locus_tag="ACHVC7_001487"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR2115576.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18523..20769)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_001488"
     CDS             complement(18523..20769)
                     /gene="ptsP"
                     /locus_tag="ACHVC7_001488"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR2115577.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20782..21312)
                     /gene="rppH"
                     /locus_tag="ACHVC7_001489"
     CDS             complement(20782..21312)
                     /gene="rppH"
                     /locus_tag="ACHVC7_001489"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR2115578.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(21665..21811)
                     /gene="ygdT"
                     /locus_tag="ACHVC7_001490"
     CDS             complement(21665..21811)
                     /gene="ygdT"
                     /locus_tag="ACHVC7_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR2115579.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21997..22686
                     /gene="mutH"
                     /locus_tag="ACHVC7_001491"
     CDS             21997..22686
                     /gene="mutH"
                     /locus_tag="ACHVC7_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR2115580.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22755..23468
                     /locus_tag="ACHVC7_001492"
     CDS             22755..23468
                     /locus_tag="ACHVC7_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR2115581.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23606..23824
                     /gene="ygdR"
                     /locus_tag="ACHVC7_001493"
     CDS             23606..23824
                     /gene="ygdR"
                     /locus_tag="ACHVC7_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR2115582.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5706 a         6535 c         5961 g         5663 t
ORIGIN      
        1 ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt ctactctccc
       61 ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg ttgccccatc
      121 ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct ggaaagtcgc
      181 cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga ccagaatgga
      241 gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat ttttatgcag
      301 cttgttgatt ttaccgagct tattcagcac tgctttacca aacttaaggc tgtcagcaat
      361 ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac cgcttttgct
      421 cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg ccgcagtact
      481 ggttgcacct ttggttgaga gcgcaaacac cgaggaaccg ctcggctggc gactggtaaa
      541 ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt ttgctacgcg
      601 cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca tattgccctc
      661 tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt tttcgcgcgt
      721 tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa gcataatgac
      781 aatcggacga tcgcgccctg ctttacctgg ttgtggacca ctttgcgctg gcggcacctg
      841 cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc
      901 ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg cgaccggcgc
      961 aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatcc gtacggtttg
     1021 cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc
     1081 cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca ccacgcgttc
     1141 agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta ccgtcacgcg
     1201 ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg cagccaggct
     1261 gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac gacgacggct
     1321 gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa gctgtgtaac
     1381 aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg tcatctaata
     1441 aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc tctcgcataa
     1501 aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc gaataatcat
     1561 gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg attccgccat
     1621 tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa
     1681 gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt gcacagcctc
     1741 ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg
     1801 catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa aacactggaa
     1861 ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct gtcgatgagt
     1921 ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg tcagtggcaa ttttattatt
     1981 gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg tatccggcgg
     2041 attgatgaag acgcgatccg tcgtcaactg gacagcggtg caatagtgct aatggggccg
     2101 gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat tgccactcaa
     2161 ctggccatca aactgaaagc tgagaagatg attggttttt gctcttcaca gggcgtcact
     2221 aatgacgacg gtgatattgt ctcagaactt ttccctaacg aagcgcaagc gcgggtagaa
     2281 gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg
     2341 aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga agatggcgcg
     2401 ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat ggaaagcgcc
     2461 gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt gattcgcccg
     2521 ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac
     2581 aaattcacca ttattcagct cgataacacg actattgcct gcgccgcgct ctatccgttc
     2641 ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta ccgcagttca
     2701 tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag cggcttaagc
     2761 aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg atttaccccg
     2821 gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca gcgtaaatcc
     2881 aaagtgttga tggcggattt agggtaagcg atgaaaatcg ccggatgcgg caggcgtaac
     2941 actcgtgcgt cgcatccggc aattacgttt atccccgcga actaaacaac gccgccagac
     3001 cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca tcggcataca
     3061 gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc gttactatcg
     3121 gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc tgcgatttat
     3181 gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc acagacttaa
     3241 tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac
     3301 cgatatcgcc attaaacaac ccaagcgcac tgtcattacg ggcaatcatc accggtcgac
     3361 cttcgtacca acgagagtgc gaatgacgat gaattttgcg cttctgttgc ataaactgct
     3421 caattcgctc attcagtcca gccacgccaa atggcccttc ccgcagggcg cacaaaagtt
     3481 ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc agcagatcca
     3541 gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct tcgccgctct
     3601 gtaaaagccg tttttcaata tcagtaaaat cctgctgaaa aacggttttc actgccgttt
     3661 tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg ccgaaacgat
     3721 agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg
     3781 ccggaacgtg agttcccgtc aggcggctta gctgcccggc acgctcggcg gtaaagccgg
     3841 cattggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag gccagttgat
     3901 cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca
     3961 tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga tgcagcgggt
     4021 taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat cggtgcaaag
     4081 tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag
     4141 ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca gccagacgga
     4201 tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc gccacggtgg
     4261 tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg
     4321 ctgccgccac tttttgccag ttaatttcat cgcttactgg aaaaagtttg tccagggttt
     4381 gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag aagcgcgcta
     4441 ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag aggatcatcg
     4501 gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct tcccaatttt
     4561 gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt
     4621 ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt aacagtgccg
     4681 ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat tgcacatcca
     4741 gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat ttcattacgc
     4801 ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc cggcgttggg
     4861 tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca ggaacagata
     4921 gataacgccg ccaaagtgac gatcatagtc gtaatcagca atgcgatggc gcagataacg
     4981 atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct gcatcgctgc
     5041 tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg atttatagtc
     5101 gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc
     5161 gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa actggcgaat
     5221 tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc
     5281 cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat tgagaggtgc
     5341 ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt caaagccgcc
     5401 gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc
     5461 aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat gcggtgttaa
     5521 cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc gtggcatcag
     5581 atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga cacgccagtt
     5641 atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag catcattaac
     5701 ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat catcgcataa
     5761 cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt gcagcaaacg
     5821 cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac ggcgcaccag
     5881 cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg
     5941 cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg cagcattaag
     6001 atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct ggacgcggaa
     6061 attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt tatggatcgt
     6121 gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg aggcattact
     6181 gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac tttccagctg
     6241 cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac gccgctcacc
     6301 gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg cccgcagcat
     6361 cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt cgactaccgc
     6421 atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca gccccatcat
     6481 tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa
     6541 cagccaaagc atttcctgcg cttccagagt ttcaaaaaca ctatcgcggt tcgaaaggta
     6601 aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct cctggcggct
     6661 gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag
     6721 caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag cacatacctg
     6781 cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca gccacatttt
     6841 catcgcaggc tgcgtttcac ctttaaagac aaaacgtaac gcctgatttt tcccggctga
     6901 tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct ggctgaaaag
     6961 cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg
     7021 ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc ggaatgcata
     7081 tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tctggctgat ggtgccagat
     7141 acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga tcattgccac
     7201 cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga
     7261 atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt tttcacgcgc
     7321 tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcacgga tcgacaatgg
     7381 ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt cccccccggc
     7441 tttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg gcaactgata
     7501 actgtttgtc tcttcttctg cccaggcgct gatcttgtcg atccatttag cctgattgct
     7561 acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca gttcacccac
     7621 tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg cgtgacggga
     7681 agctagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc cttgcagata
     7741 acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga cgacctgggc
     7801 tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg cctggtagcg
     7861 tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt caaaggcatt
     7921 caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt catccatctg
     7981 ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct cttccagcag
     8041 gtgttcgtac aatggattgt cggtggtttc acgcagacag gcgatgcgca actcgtggat
     8101 attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag
     8161 cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag
     8221 gcgcaaataa agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag
     8281 gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat cactcattct
     8341 tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac actttccagc
     8401 cttcaggatg cacatattcg gctttcccgt tttggctgcc ggaaatctgc gacaggatag
     8461 ccatgccttg cggctcgact accgcctgat ggaagaaatc ggccagtttt tgcggcgtca
     8521 gcagtttaat ctgggccacg attttatcac gcgaatcgaa gcgcatattg ccgcgatcga
     8581 aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg
     8641 taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc aattttgcct
     8701 ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc
     8761 tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac gcaaacacgg
     8821 catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt acgatctgcc
     8881 ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt acaaataccg
     8941 ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat tgttttttat
     9001 cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac tgtttttgca
     9061 catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc ataaactctg
     9121 gtcgagcccc tgattttaag gcgtcgcgat aagccagcac ctctttcaac gtaatggagg
     9181 gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg
     9241 gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc tggttatacc
     9301 aggacttcgc ctgctcaagc tgatcttccg tagcggtata gctaaagtac ccctcgagca
     9361 atgcctggaa cagctgcggc agacgctggg ggtaaccatt agcattaacc ataaggccgt
     9421 tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt aactgatcaa
     9481 gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg gcgctgtcca
     9541 tggctttcgg attacgcaaa atcaggctga catcagcttt gggttcgctg gaaaaataac
     9601 ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct ggatggtcat
     9661 atttcttctc tgacttaatc agcgagaaat cgtcaggaat ataggggtta agctccggta
     9721 aggagagcgc aatattggca gctttttgct gccagtcagc gaaagtttgt tcgctgattt
     9781 tatcgacctg atacggcgca tcgacaaaat aagccgtttt attgtgcggc tctttcgggc
     9841 tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttct tttactgctt
     9901 tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaacc ggaacgcgaa
     9961 tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac ggataacgga
    10021 agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg cctttttcac
    10081 gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta gccaggcctt
    10141 tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg ataggatcgg
    10201 agttggcgct aatgccctca actaatccct gcttttgcag ccagtcggaa agtgtacctg
    10261 ggctgcgatt accaatcaga taggtaatca attcatccgt tttactacgg aacttcgctg
    10321 agttgttatc gatgcgaaac tcaacgcgca gaactttacg cggcattgcc gggacgtaat
    10381 gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc
    10441 tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg
    10501 gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc tcgtggaaat
    10561 ctttcagcgc ctgctgcacc ggattaccag gtttatcgct taaagtttcg aggttaccac
    10621 cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc
    10681 gcatcccgtc acgcgtacgc gccatggtta attcagcgtt caccgcatta cgctcacgtt
    10741 cggcatattt cttgtcgagc aaaggttcag caatagcatc ggccaggcgg tctaccgcac
    10801 caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc gcagtgctgg
    10861 cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc tgcgggtact
    10921 ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg
    10981 gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact gcctgcggat
    11041 cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac tggcggttat
    11101 ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct gcctgactta
    11161 agggtgccca aagggcaact aacaacaata atgctttgaa ccaggtgctg cggggcattc
    11221 tcggacctca taatcaactt aattttctgt ccagattcaa cacgttaacg caaatcatct
    11281 gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca tcagtccgtg
    11341 actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacag cactcatgac
    11401 tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat ggcctccatt
    11461 gtctctggtg ttaattgccg ccagagcctt tgataccaga tatcatcacc ttcgccacgc
    11521 accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg caacgtggaa
    11581 tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca cgcgccgcca
    11641 ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc aatcagttgt
    11701 gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc gccgtctttg
    11761 cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag gtgttccagc
    11821 caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa caggccatcc
    11881 ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag atcaatttcc
    11941 atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg catctcctgg
    12001 cactgagttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg
    12061 aagcgacgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa taactgctga
    12121 ttgatttgat aacggctaag cccttccaga ataaatggct cggtgtcggg gatttcgctg
    12181 tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc
    12241 cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg cagcggctga
    12301 acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc acgagcatag
    12361 ctttgtcgtt cgcctggctg atagttttgt ggatcaaacg gcattcgggt atggaggcaa
    12421 gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt
    12481 agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga cgggaaacgt
    12541 tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg ctgcgcggaa
    12601 attaacgctt ccaggaacag atagcggtcg tcatcgcgac ggctacggtc gccacgcttc
    12661 ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata aacgccgtcg
    12721 ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat cagagtacaa
    12781 atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag acgctgtgcc
    12841 agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc taaaccttcg
    12901 gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc
    12961 ggcaggaaga aggcgctgag catatcgcga caaaccggca accactcttc tagcggacgc
    13021 tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc cagatgcccc
    13081 accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg ccactcgccc
    13141 tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt
    13201 tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc ccaacgaatg
    13261 ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc
    13321 gccgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa gcggctgtct
    13381 ggcaacgata acaggctgat aaacgcttcc agtaccggat gcgactgccg cgcccgacgg
    13441 tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac agcccgaata
    13501 aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc
    13561 gggtcttctt ccagcatcgc cagcaggcga tcgtgcaaaa cttcaacttc acgctgcggg
    13621 ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg tttgttatcg
    13681 ctacgggaaa actcttcgat gttcacacca gcaacggcac ggttttccag ttccagaatg
    13741 tcagactgaa tgttatgcag caggttatct ggcgtgacat cgacaaaggc gtccagctcc
    13801 tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt accccatgaa
    13861 gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg cccggcattt
    13921 tcgctatcac gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg ctgacgggtc
    13981 agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg gcaggggttg
    14041 gtaaacagga gatggatttc aatatgttta cccagcgcct gtagcgcctg gagataaaca
    14101 ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc
    14161 gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg ccagcgcggt
    14221 tgcccgagtt gatgggtata ttccaccagc gccttccaca acggggcttg ccaggcctgt
    14281 gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca gtccggacga
    14341 tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa cagttttcgc
    14401 ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg ctccagcaat
    14461 tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc gctctctttg
    14521 gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg cagcggaaaa
    14581 tcaatgtttg ccgcaatact aaacttttgc gacagggtca tttgcagcca ctgcgccata
    14641 ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg ttcgcgttcg
    14701 acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta gacccttaac
    14761 atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc gccttctctg
    14821 cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt
    14881 cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca
    14941 tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa tgttcgctgg
    15001 atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat tacctccggc
    15061 aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct ttcagcggac
    15121 aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcaatgact tcgcctgttc
    15181 gccacaacga aacgccttca taaccggcaa ccaataaggc ttcattatct gccagtaaac
    15241 gcaaacaaac ccgggcatcg gttccagcgt actgcgagca ctgaactgcg ggctgcgtct
    15301 gccaggagtg cattttcccc cacgccagcg ccgactgaac gatggcctgg cggcgcaatg
    15361 actgactttc tatgctcacg cgagaggcaa aactgcgatc ctgctgactc attccttgca
    15421 atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa acaccttttt
    15481 cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg tttgcggttc
    15541 agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc tgacatcctg
    15601 acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt tatcccagcc
    15661 cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct tcagacgaaa
    15721 tccaatctgg tcagactctt ttaccggttc gcgatcccag ataccgttac tgttcgcatc
    15781 ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc cggtacagct
    15841 gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc gcagccagat
    15901 ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta acgcaggcag
    15961 aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca acacttccag
    16021 cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg acaaagccgg
    16081 agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt gaagcgaata
    16141 tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc
    16201 atttagactt cgggccagcg tggcacaaag accaatggtg aactgccatg acaggtattg
    16261 gccccagcaa cggaactcac aaggcaccat aacgtcccct ccctgataac gctgatactg
    16321 tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg
    16381 ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata gagtccactt
    16441 gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt ataaccacgt
    16501 tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc agtttctatt
    16561 tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag
    16621 gcgtctcgaa gaatttaacg gagggtaaaa aaaaccgacg cacactggcg tcggctctgg
    16681 caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg gatcgtagcc
    16741 ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac gtttgataat
    16801 caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat ccatatgatt
    16861 gctgtacagg tgcgtatcgc caccggtcca gacaaaatca cccacttcca gatcgcactg
    16921 ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa
    16981 gacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca catagaactg
    17041 gaagaatgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt tccacgctga
    17101 aacaataatg cggcgggaat ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg
    17161 gtcaatatga cgaccatctg gcgttggcca ggcgcgccac tgtttaccat acactggtcc
    17221 gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt cgtgtagata
    17281 agcaatgtta gtgtcgccct gcagaaacca cagcagttcg tggatgatgg aacgcaggtg
    17341 gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc
    17401 aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac
    17461 tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt gctgtgggct
    17521 gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga tggaaagaat
    17581 ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa
    17641 aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag agacagctcc
    17701 cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca caccttccag
    17761 cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga
    17821 ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc ctgggaacag
    17881 catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc
    17941 cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag aaacctggaa
    18001 gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc caatcaggcc
    18061 gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat cggccataaa
    18121 ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga cgccgaggaa
    18181 gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg gacgattcgc
    18241 ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca ggccgtacca
    18301 gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa aatgcagata
    18361 gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa cagcgattga
    18421 aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg
    18481 ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc ctccgcgaat
    18541 cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc
    18601 cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat caatgcgccg
    18661 cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc gatacccaag
    18721 cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca aacggagatc
    18781 gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca ttgcaggatg
    18841 aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca gaatgtattg
    18901 agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat gcggcagcat
    18961 aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc
    19021 gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt catcaacttc
    19081 atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg cattagcacg
    19141 cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc
    19201 acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct gcttatctgc
    19261 tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca gcatcccctg
    19321 atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga atgggatttc
    19381 agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat gttccgggct
    19441 taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg cgggtaaatt
    19501 gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc gctgatattc
    19561 ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat caacgatcag
    19621 cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag ggatccccag
    19681 cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga caacaccgac
    19741 taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg ccaccagaat
    19801 gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa acagcaatcg
    19861 ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc ttagcgcggc
    19921 aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca ccgagccttt
    19981 atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagtaggt gcgagtaaag
    20041 atcgaaaata gccgccgttt ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg
    20101 aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc
    20161 cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct gccagccttc
    20221 ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc
    20281 aaacaaggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg caagcgtcac
    20341 caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac
    20401 accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc
    20461 ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg
    20521 ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg ttacagtgcg
    20581 accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc gatcatgatc
    20641 ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat
    20701 atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg
    20761 agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg tgttttgtgg
    20821 ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg
    20881 gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac ttacccatcg
    20941 ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat cgccgctcac
    21001 cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg
    21061 caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac
    21121 gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga
    21181 ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc
    21241 ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc
    21301 gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact
    21361 ccaagaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa ttgaataact
    21421 cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt tattcacctt
    21481 ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg gagcattctg
    21541 aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt acatgtaatt
    21601 tgagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac gggttggcga
    21661 aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc actgaaccac
    21721 taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt ggaggttttt
    21781 cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat tttcagtgac
    21841 cagatttgga aaacctattg cagtgttgcg caactcgatt accggcagcc taaaaagcaa
    21901 tataaatcag agaattgaac aacacatgtg gaaaaagtta cactgcgaat attcggcaca
    21961 taattgctgt ttgtttttta accaaggtat catgacatgt cccaacctcg cccactcctc
    22021 tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc tggttataca
    22081 ttgggagaac tggcggcact tgccgggctg gttacgccag agaatttaaa acgcgataag
    22141 ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag taaacctgag
    22201 caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag tcttggtcgt
    22261 ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg
    22321 gaaaccagcc atgtgcgcca caagctcaaa cgcgtactgt ggataccggt tgaaggtgag
    22381 cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa
    22441 gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatgat tgttctcggt
    22501 caggttgagc ggatcaccgc tcgtcacggg gagtatttac agatacgacc gaaagcagcg
    22561 aatgcgaaag cgcttaccga agccattggt gcccggggcg aacggattct gacgctgcca
    22621 cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca ttttctgatc
    22681 cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt catttttttg
    22741 gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg cttgcgctcg
    22801 gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc ctttctctgg
    22861 tggtggcaaa gctccccaca gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag
    22921 ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc ctgacaaatc
    22981 cgctttttac aatattcagt caggaaattt ccgcccgtga tttgattctg cttctgggtg
    23041 gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa ggtgaagaag
    23101 aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt atgctgctgg
    23161 atattatctt cagcctcgac tcggtgatta ccgctgtggg tctgtcagat cacctgttta
    23221 ttatgatggc cgccgtggtg attgccgtag gcgtgatgat gttcgccgcg cgctcgattg
    23281 gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc ctgattctgg
    23341 tcggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt tacatctact
    23401 tcgcgatgtt cttctctatt gcggttgaaa gcctcaacct gattcgcaac aaaaagaacc
    23461 cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactcctgt taccctcaca
    23521 gtttaagatt ttcctgcttt caagatatat aacgtcggtt tataaacaga ctattatcat
    23581 aggtgggcca aatgaggata accggatgaa aaaatgggca gtaataattt ctgcagtcgg
    23641 actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca aagatggccg
    23701 tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg tgagttatca
    23761 cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg
    23821 ttaacaaata aggtcagcat ccggctgtaa tgccgatcag ttaag
//
LOCUS       DAZPLM010000044        23609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPLM010000044 DAZPLM010000000
VERSION     DAZPLM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.044"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            433..1599
                     /gene="sopA"
                     /locus_tag="ACHVC7_001494"
     CDS             433..1599
                     /gene="sopA"
                     /locus_tag="ACHVC7_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR2115583.1"
                     /translation="MSLKKLLQDCVTRGQEMTQAIAIAQFGDDSPEARRITRRWGITE
                     VADLIGVTPQAIRDAEKSGRLPAPDFEMRGRIERRAGYTIDQINSMRAVFGNPNQRPQ
                     DKNPVALAVMSHKGGVYKTSSAVHQAQWLALQGHRVLLVEGNDPQGTASMYHGYVPDL
                     HIHAEDTLLPFYLGERDNAEYAIKPTCWPGLDIIPSCLALHRIETDLMQYHAQGKLPH
                     PPHLMLRAAIESVWDNYDVIVIDSAPNLGTGTINVVCAADVIVVATPAELFDYASVLQ
                     FFTMLLDLLESVDLGGFEPTVRLLLTKYSLTTGNQSRWMEEQIRNTWGSMVLRQVVRV
                     TDEVGKGQIKMRTVFEQAANQRSTLNAWRNAVSIWDPVCREIFDDLIKPRWENE"
     gene            1599..2564
                     /locus_tag="ACHVC7_001495"
     CDS             1599..2564
                     /locus_tag="ACHVC7_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR2115584.1"
                     /translation="MKQRSLLKNAPDINRIMSNNHHAPEHQPVSPMVGDLKRQLSSLT
                     GNSITLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNESSLADLIPSFLTSG
                     QQNPAFARKVSGIVEVADGSRRRKAAILTGCDYRVLIGDLDDEQMHWLSQIGNDYRPI
                     SAYERGKRYLRKLNDFDGNVKALAEAENIDRNIITRCINTAGLPKDILAIFNHPGELS
                     ARAGDSLFKVYKKNMAAMSNAAHHLLAIKKNGEDLEASRIIQILSDSVLVGDEEKTKD
                     EKKYGEGITARYKGNFVTIKIDSRKISKEILSRIELLLEEQSTDN"
     gene            complement(2597..3172)
                     /locus_tag="ACHVC7_001496"
     CDS             complement(2597..3172)
                     /locus_tag="ACHVC7_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2115585.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSLSPDIEWPSQP"
     gene            complement(3172..6570)
                     /locus_tag="ACHVC7_001497"
     CDS             complement(3172..6570)
                     /locus_tag="ACHVC7_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2115586.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVDMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(6635..7234)
                     /locus_tag="ACHVC7_001498"
     CDS             complement(6635..7234)
                     /locus_tag="ACHVC7_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR2115587.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKRHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(7301..10699)
                     /locus_tag="ACHVC7_001499"
     CDS             complement(7301..10699)
                     /locus_tag="ACHVC7_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR2115588.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASSNIKPGYDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLNFYKGLINKTHLGKELLENFELTEDNASRLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(10760..11332)
                     /locus_tag="ACHVC7_001500"
     CDS             complement(10760..11332)
                     /locus_tag="ACHVC7_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR2115589.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(11329..12072)
                     /locus_tag="ACHVC7_001501"
     CDS             complement(11329..12072)
                     /locus_tag="ACHVC7_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2115590.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(12078..12776)
                     /locus_tag="ACHVC7_001502"
     CDS             complement(12078..12776)
                     /locus_tag="ACHVC7_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR2115591.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(12776..13105)
                     /locus_tag="ACHVC7_001503"
     CDS             complement(12776..13105)
                     /locus_tag="ACHVC7_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2115592.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(13102..15663)
                     /locus_tag="ACHVC7_001504"
     CDS             complement(13102..15663)
                     /locus_tag="ACHVC7_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2115593.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(15656..16090)
                     /locus_tag="ACHVC7_001505"
     CDS             complement(15656..16090)
                     /locus_tag="ACHVC7_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR2115594.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(16072..16494)
                     /locus_tag="ACHVC7_001506"
     CDS             complement(16072..16494)
                     /locus_tag="ACHVC7_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR2115595.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGSAEPVSAGKCSTVS"
     gene            complement(16510..17250)
                     /locus_tag="ACHVC7_001507"
     CDS             complement(16510..17250)
                     /locus_tag="ACHVC7_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2115596.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(17258..17653)
                     /locus_tag="ACHVC7_001508"
     CDS             complement(17258..17653)
                     /locus_tag="ACHVC7_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR2115597.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(17650..18228)
                     /locus_tag="ACHVC7_001509"
     CDS             complement(17650..18228)
                     /locus_tag="ACHVC7_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2115598.1"
                     /translation="MVIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLPRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(18240..18593)
                     /locus_tag="ACHVC7_001510"
     CDS             complement(18240..18593)
                     /locus_tag="ACHVC7_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR2115599.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(18605..19000)
                     /locus_tag="ACHVC7_001511"
     CDS             complement(18605..19000)
                     /locus_tag="ACHVC7_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR2115600.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDGT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(19042..20067)
                     /locus_tag="ACHVC7_001512"
     CDS             complement(19042..20067)
                     /locus_tag="ACHVC7_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR2115601.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(20123..20455)
                     /locus_tag="ACHVC7_001513"
     CDS             complement(20123..20455)
                     /locus_tag="ACHVC7_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR2115602.1"
                     /translation="MTSKEIFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(20465..21784)
                     /locus_tag="ACHVC7_001514"
     CDS             complement(20465..21784)
                     /locus_tag="ACHVC7_001514"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR2115603.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSVNDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(21765..23366)
                     /locus_tag="ACHVC7_001515"
     CDS             complement(21765..23366)
                     /locus_tag="ACHVC7_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2115604.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(23363..23569)
                     /locus_tag="ACHVC7_001516"
     CDS             complement(23363..23569)
                     /locus_tag="ACHVC7_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR2115605.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
BASE COUNT         5081 a         6938 c         5851 g         5739 t
ORIGIN      
        1 gttatgttct tgattggtgt tttcatttga tgtaatgttc tcagtttgcg tactgtttta
       61 tttaacattt gtttttattc tttatttatg ttctttattg tgtttgtgtt attacttgtt
      121 ttttatgttc tttatttgtg ttcttatgtt ggtaaggttg ggagaactaa tagagaaaac
      181 gtatctcatt gatgattctt atcatgtttc tctagtttta tatgatttcc tttcttaaga
      241 aaggtcataa atatcaatta gttatgatta tattgttgtt cgttttatgc atttcgggag
      301 gggaaaaaaa gatgatttag ctcaaatatt cgcctttgcg tttttcgttt tgctttgcct
      361 tttttatgca aatttgctag aaataagcaa agatttaatt attaagcaaa gttacaagct
      421 ggagatgaca gtatgtcttt gaaaaagtta ttgcaagatt gtgttacgag aggtcaagaa
      481 atgacccaag caattgctat cgctcaattc ggtgatgata gtcctgaagc tcggcgtatc
      541 acccgccgat ggggtataac tgaagtagca gatttaattg gtgtaactcc tcaagcaatt
      601 agagatgctg agaagagcgg gcgtttacct gcacctgact ttgaaatgag aggaagaatt
      661 gaacgtcgtg ctggttatac catagatcaa atcaacagta tgcgagcagt ttttggcaac
      721 ccaaaccaac gaccacaaga taaaaaccct gttgcgcttg ccgttatgtc acacaaaggc
      781 ggcgtttata aaacatcgtc tgcggtacat caagctcaat ggttagcatt acaaggtcat
      841 cgtgttcttt tggttgaagg taacgatcct caaggcacgg cctctatgta tcacggttat
      901 gttcctgatt tacatatcca tgccgaggat actttactgc cattttactt aggagaacgc
      961 gataacgctg aatatgctat aaagccaaca tgttggcctg ggctagacat tatccctagc
     1021 tgcctagctt tacatcgtat agaaacagat ctcatgcaat atcatgctca gggtaaattg
     1081 cctcatcctc cacaccttat gctgcgcgca gctattgaat cagtttggga taactacgat
     1141 gttatcgtta tagatagtgc tccgaacctc ggtacaggaa ccattaacgt tgtttgtgcc
     1201 gctgacgtta ttgttgttgc tacacctgca gaattatttg attacgcttc tgttctacag
     1261 tttttcacaa tgcttctcga tctattggaa agcgttgacc taggtggatt tgagccaacc
     1321 gttcgcttac ttctgactaa atatagtctc actacaggga atcaatcacg atggatggaa
     1381 gaacaaataa gaaatacatg gggatcaatg gtattacgtc aggttgtacg cgttaccgat
     1441 gaagtaggta aaggccagat aaaaatgaga acagtatttg aacaagcagc aaaccaacgt
     1501 tcaacattga atgcatggag aaatgctgtt tctatatggg accctgtttg ccgagaaatt
     1561 tttgatgatc taataaaacc tcgctgggag aacgaataat gaaacagcgc tctcttctaa
     1621 aaaatgctcc agatattaat cgcattatga gtaataatca tcatgcccct gaacatcagc
     1681 ccgtatcacc tatggttggt gatttaaaac gtcaactgag ttcattaacc ggtaacagca
     1741 taactttgcc agtatgcgga cgtaatgtta actttaagtt agagacaatt cccgcagata
     1801 aagttgaaat ggcaacaatg gtatggctag gtaatgaacg cgatcaagat ttattaaatg
     1861 aatcctctct agccgattta atcccatctt ttctaacgtc tggacaacaa aatccagctt
     1921 tcgccagaaa agtatctgga atcgttgaag tcgctgacgg ctcacgtaga agaaaagcag
     1981 cgatattaac aggatgtgac tatcgcgtct taattggtga tctcgacgat gaacagatgc
     2041 attggttatc ccaaattggt aacgattata ggcctattag tgcatacgaa agaggaaaga
     2101 gatacttacg caagctaaat gactttgatg gtaacgttaa agcattagcc gaggctgaaa
     2161 atatagatag aaatattatc actcgctgta ttaatacagc tggtctacca aaagatatat
     2221 tagctatctt caatcatcca ggagaactgt cagctcgtgc tggagattct ttgtttaagg
     2281 tttacaaaaa aaacatggct gccatgagca atgctgctca tcatcttctg gcaattaaaa
     2341 aaaacggcga ggatttagaa gcttcacgga ttatacaaat attatctgac tctgttctgg
     2401 ttggcgacga ggaaaaaaca aaagacgaga aaaaatatgg ggaagggatc acggctagat
     2461 ataaaggtaa ttttgtaact ataaaaattg atagccgcaa aatatctaaa gaaatactaa
     2521 gcagaattga gttgttacta gaggaacaat ctactgataa ttaagaacta tcccctgcat
     2581 tgcaggggat attgatttat ggttgacttg gccactcgat atctggagat agtgatgtat
     2641 caacacggtt cagcaacacc cgatacttct tccaggcttc cagcaacgag ttttcttcct
     2701 ccgttgcaat ttccagatct gcagcatcct gaagcggcgc aatatgctca ctggctacct
     2761 gcatcaggtt gttttttgtt tcttccgcct cccggatccg gaacagtttt tctgcttccg
     2821 tatccttcac ccaggctgtg ccgttccact tctgaaactc cccttccggg gataaccagg
     2881 tgacattttc cggtaatggg ccaagttcag aaataaataa cgcgtcgccg gaagccacgt
     2941 catagacggt ttttccccga tggtcttcaa caagatgcca cgatgcctca tcactgttga
     3001 aaacagccac gaagccagcc ggaatatctg gcggtgcaat atcggtactg tttgctggca
     3061 gacctgtatg aggcggaatg tatgcgtcac cttcaccaat aaattcatta gttccggcca
     3121 gcagattata aattgttatg gtccgtggtt gttcactcat tctgaatgcc attatgcaag
     3181 cctcacaata tagttaaatg cgatgttttt gacggtgttt tccgcgttac cagcagcgtt
     3241 aacggtgatg gtgtgtccat gtgagccaat cgcaaccgag tgcgtatgag caccaatacc
     3301 gacagtatgc gcgtgtgcac ctgcagatgc tgctgtgccg gacagtgagt ggctatgatt
     3361 accatctgta ctggtattag ctaaccaccc cgtagacata tctactgagc cttgtacacc
     3421 ccaggtattt tgacctgagc ttgtataacc atattgataa gtatctttaa aaacactggg
     3481 gttaaatcga cggccatctc tatggctgtg attaccagct gcattcgtgc tgccacttaa
     3541 actatgggta tgcgcaccag tgttattcgt ggatttagtg ccgtaatcaa acgacgatgt
     3601 ggttttcgtc cccaaatccg tactggatgc gctggcgctg tgggtgtgcg atttaatgcc
     3661 gtcctgttcc tgagacaata cggcccgacc actggcaggt tttcccttaa tcgtccagcc
     3721 acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt aagcagattt
     3781 gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg atggccacgg
     3841 gattggtgcg ccgactgggt agctttctgg tggaagattt ttcgaggtat aaacttctgc
     3901 ccagtcttcc tcaaaaccat aaccgtctct tgaagaacgg tagaacagac caccatttct
     3961 gtaatgcgcc ttcatctgca aggtccggca acttccgact ccggtataga agttaaccag
     4021 aatatagctg tcgccagagc gggtgacatt gtaagcgcct gattcggcat tccagggaac
     4081 gccaccatcc gcatcggcat atgtatccgt tgcccttctg gcaaaagcag ccacatgcgc
     4141 ggcggttaaa gtaatatctt tggaaccatc aaactcaaca ccagaaacca gtcttggcgt
     4201 ttgcagcttt gttgctgtta atgcattacc gttcagactt gcggacagtt tggttccaat
     4261 aaccagttcg ccggttgcgt tatcaatagc aaacggtctt aatgtattcc agccaccata
     4321 aacatcacct tgattggtaa gcagcaggta agttttagcg ccatcattac gccataatgc
     4381 accatactcc ccacctatca ttcgaatctg attaccacca cgcgctacaa tttcgtctgt
     4441 ggcaaaaagt tttttgcacg acaagttatc gttaacgatt aacgaatgag actcataaaa
     4501 accacgccca ctcttaaaat caaggataac gtccgccgcg atacattcag tcgctggatt
     4561 tgttgcccca aacttatagg tcgtatcatt aacaacgaga tcagcaccag gtgcggatat
     4621 tgacaggcca tcttcgataa acgcaaaaac agggaaagca gcgccatcaa catagaacac
     4681 agagcgcaaa tcatcgccct tattactcat cattattgag tggatggctc gttcattgtt
     4741 ttgatattgc cagaacattc cataagcata acgccccctg tcagtccagc caccaggcat
     4801 aacaaatccg ttaaactcgc agttattcat cggatcgcct gcggttcgcg ttgccgtggt
     4861 gataatgacc cttgatgcca gttcgcttac tgagccagca gaacgcataa caacaacagg
     4921 gtaatatttt ccagatgttg cacctgcagg agcgttaacc cgcacataac gcataccacg
     4981 cttatcagca aagtctgttt tactgaccgc gttaatgttg ttcaggaagc gtcccttatc
     5041 gggtatatca gcgccgttct ggtctttctg cagacgtttc tctgcattgt cataggctga
     5101 ttttactgcc tttggcgttg ccgccagcgt ttcagacgta ctgttggtcg cactgctgag
     5161 ctgtactatc ccctttttcg tcgtacttgc atcctcaagc gccacggcgg atgcaatatc
     5221 ctctgcccgt ttagctgctg tctcggcgcg cgttgccgcg gattccgccg tacttttgct
     5281 ctgagctgcc gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg ctgtcgtggc
     5341 gctgcccttc gctgctgacg cctgtctggt cgcctcatct tttgaagcag acgcagatga
     5401 tgccgatgac gccgccgaac tggctgacga tgcggctgcc gtttttgagg attctgcgct
     5461 ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagctg acctcgctgc
     5521 tgccgtggcc tgttcagtgg cttcgccagc cttcgttgtg gctgttgaag cggatgatgc
     5581 ggcgctttct gccgactttc cggcagcggt ggcactggct gaggcctgcc cggcatttgt
     5641 tgacgcggcg ctggcagatg aggcagccgc tgtttttgag tctgctgctg cggaggcact
     5701 ctgtgctgct gccgtctcag aagacttagc gttcgtctcg gacgtttttg ccgccttcgc
     5761 ggaattgcct gccgctgttg ccgaggaggc tgcactactg gcgctcgagg ctgcgctcgt
     5821 ttctgatgat tttgccgcct cttttgaagc cgacgcatct ctggctgagg tggcagcttc
     5881 tgacgctttc gtggtcgcgg tggatgcaga agtggcggct gattgttgtg acgctgaagc
     5941 attcgtttct gacgttttcg ccgcaccggc actggtagcc gccgcgcttt ttgaggactc
     6001 tgcagcggca gcactttttg atgcttcagt ggcctttgtt gatgccgttc ctgcgctgga
     6061 agacgctgac tgagccgacg acgcggcctg tccggctgac gtgctggctg cgcgtgctga
     6121 gtccgcagca tcagccgcat gggttgccgc ctcacgggct gatgtgctgg catcactggc
     6181 tgacttcttc gcggctgccg tgttctgtgc caccgcggac gcgttacgcg ccacctcttc
     6241 caccatcagt tcaaaacggc gcagtgcctc cggacgggca tcatcctccg tcatggcacc
     6301 gagaaaatca ttcagcgtac cgggttggga atcttcatac acggtgatgg tcccggcatg
     6361 tgacggcggg aatccttcca ccaacagaat aacgctgtac tgaccgtact caacgtccat
     6421 gctgtaacgc ccggcttcat ccggattttc tgaggccagc gtgttcacca ccaccgtggt
     6481 gctgttacgt tttgctttca gctggattgt gcagttctgt accggttttc ctgtgccatc
     6541 tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc cgcctgaacc
     6601 ggcgggctgt cataacactg tgttacctgg ctaatcagaa cttataaccg acacccatga
     6661 tgaaaccgtc agtgcgccag tcgccactgc cggagccttc ataagcaata tcaatggcca
     6721 cggattcggt cgggttaaac tgcacgccag ctccccacgc cagagacgtg ttgctgtggc
     6781 gaccgtcatc acttccggtc agcacgtcgt gcctttttcc cttgttgtca gttacgcgga
     6841 gataatcccc ggaaaaagtc gacacacggc tgtaagccac gcctgccatc gcatacgcgc
     6901 tgaaccattc attcacgcgt acagacggcc ccgccatcac gctgaaccag cggttacgca
     6961 cggaatcttc atgccagcgg gtatcgctgt aatgggtgag ctggcgattc ttgtctcctg
     7021 catagctgaa cgacgtcacc atccccagtg tgtccgtaaa ctcataacgg tatttcacgt
     7081 taatcccgtt cagatcatca ctgccgggaa cgttcgtccg ggcatgaaga taccccgcgc
     7141 tcagcgtgga ctgatgttca gacgcccatg caggcgcacc ggatacggcc agacaaatgg
     7201 ctgcggacaa aatggcggca taaagtttac gcataattac ctctcgcttt tctgcaataa
     7261 aaaaggcgtc atttctgacg cccgttctgg gttataaaat tcagctgata ctgatacctg
     7321 ctgtggattt tttcatcacc acaaccagca gatcgctgat actggttgtt ggtgtccagt
     7381 tattcgctcc tgatgaagat acggtgaatg tcagtgtcag cgtcccctgt ccggcaggca
     7441 tatctataac tgaggaaaat acgccctgag catccgtcgt ggactgatta aaaatctcct
     7501 gaccattgcg ggtcactctt aaccggcagg ttgaatacca gtatgactgt tggttattac
     7561 tgttgaaatt ctcatgctta ccaccgcgga ataacactgg cggtatcatg acctgccggt
     7621 caaatttctg atcatcactg attcttaccg tgatggtgcc actggcataa ctgttcgtgc
     7681 gggggaaaga cttgctgacc gttttgacaa tatcaccttc aatctggttg gctgacagtt
     7741 tccccttaat ctgacagttc tcattaatcg tgacgttgtt gagcgtcccg gagttcgcat
     7801 tcacactgcc actgatatcc gcatttttag cggtcagctt tccgtccggt gtcagggaaa
     7861 aggccggagg attgccgccg ctggtaatgg tgggggccgt caggcgcttc aggaacacgt
     7921 cgttcatgaa tatctggttg ccctgcgcca caaacatcgg cgtttcattc ccgtttgccg
     7981 ggtcaataaa cgcgatacgg ttagcggcaa ccagaaactg gctcagtttg ccttcctccg
     8041 tgtcctccat gctgaggcca atacccgcga cataatgttt gccgtctttg gtctgctcaa
     8101 ttttgacgcc ccacatggca ttccacttat cgttggcgtc cttccactct ttcgaaaact
     8161 cctccagtct gctggcgtta tcctccgtca gctcaaagtt ttccagcagt tcctttccga
     8221 gatgcgtttt attgatcaac cctttataaa aattcagata accttccgca tcatcgctcg
     8281 cccgaccgac ggcctccaca aatgccgatt taccaacggt attcacgctg cggatataaa
     8341 agtaataatc ataacccggc ttaatattgc tactggcagc tatccagtac agcgccgtgc
     8401 caagatagcg ggctgtggtt tcaacctgcc tgatatccgc aatccgcttt tccgagaacc
     8461 agaactcaaa ctgcaccgtc gggtcatata cagccagttt cgggaccgct gttatctgaa
     8521 aataccctgg tatcagttca atagtgacag gcgctgccgg tgcggcaatc cggaacgata
     8581 ccgatgcagg atcgccctgc tgcccccacg catttgccgc ccggactgtc agcctgtagt
     8641 tccccagcgc cagttgcgtg aagcggtatg tggtttccgt cgtccgggcc gtgctgacca
     8701 gccgctcact gccgtcatcc gctgccacgg tcaggcgaag cataaagctc acgcccttca
     8761 ccaccttcgg cgtgtcccag cgcgccagca cctgatattc cccgctgtct gcggtgactt
     8821 cggcagtcag gtgctgcacc gcgggcggcg tgacgccatt caccgtgccg ctctggtcgc
     8881 cgtcaaagtg cgccccgtta tccacgatgg cttctttttc gggtacatgc tgcacggcgg
     8941 tgatggcata cgtgccgtcg tcgttctcac ggatactcac gcagcggaac aggcgctggc
     9001 gcaacgtcgg caacttcagc ccccacacgc tgtattctgc aacgccgtca ggaacacggc
     9061 tcacttttac cttcacgccg tcggtgacgg actggacctc cacgctgacc ggattgccac
     9121 ttccgtcaac caggcttatc agcgtggtac cggaggatgg cagcgtgatt tcacggtcga
     9181 gcgtcagcgt ccgggtctgg ctgtttaccg ccagcacgcg cccgccgatg ctgataccgg
     9241 catagtcatc atcgcagatt tcaatgacat cgcccggtac atggcgaagc ccttcggcac
     9301 ccacgctgaa gtccacggtc tgcgtttcca gcagttccgt tttaatcagc cacagcccgg
     9361 cgcggtgtgc ctgcccccga ctggtacagc caaaggcatc catcttcgtg acattacgac
     9421 cgtaacgggc aatggcctgc gtgtcctcca caagctctgt tgccgtttcc cagccgttat
     9481 ccgggtcaat ccagttcacc tcaacggcat tatggcggtc cttcagggcg ctgaaactgt
     9541 agcggaacgg cgcgccatca tccggcatca ccacattact gcggttatag gtccacacct
     9601 tatccgacgg tcggtcctgc acgaacgtca gcgtctgccc gttccatacc ggcatacagc
     9661 gcatcgccga gcagaaatca ctgagcacat cccacgcctt gcgctgtgtg gtcagccagg
     9721 cattacaggt gatgcgcggc tccgtgctgc caaagccgtc cggcaccgac tggtcacagt
     9781 actggccgat gacatacagc gcccatttat ccacatctgc cgcaccaaga cgtttcccca
     9841 tgccatagcg cggatgggtc agcatatccc acagacacca ggccatgttg ttgctgtatg
     9901 ccggttttaa cgttccgtcc cagataccgc tgtattgccg cgtctgcgga ttatagttcg
     9961 acggcacctg cagaatgcgc ccgcgaagat gataattacg gctcacctgc tggctgccga
    10021 actgctccga atccacctgt acgccgacca gtgccgtgtt cgggtagcac tgtttcacat
    10081 cgatgatttc ggtgtatgac gaccagagcg ttttgttctg cagctggtct gtggtgctgt
    10141 ccggcgtcat tctgcgcatc cggatattaa acgggcgcgg cggcaggtta tccaccacca
    10201 ccgaggccag atactgcgag gtggttttgc ccttaatggt gatgtctttt tccgtcaccc
    10261 agccaccgtt acgctgtatc tgaaccagca ggcgaacttc agacggattc ctgtctccct
    10321 ttgaggtggt ttccaccagt gcctgtacac cgaaggtaaa acgcagtcgg tcaatgtttg
    10381 ccgacgtgat ggtccgggtg atcggcgtgt catatttcac ttccgtaccc agcaccgtct
    10441 cggagccgga ggattcaaat ccctccggcg gtgactgctc ctgctcacct gcccggaaca
    10501 ccaccgtgac gccggagatg ttggtattcc cctcactgtc cagcaccggc gtactgttca
    10561 gcagcacgct ttttaatcca tccaccggac cttcaaccgg cccttcgctg atggcatcga
    10621 tcacactcag cagctgcgtg gacttcaggt tgtccttcgc ttcgcgcggg gtatgcccct
    10681 tactgctgcc tttacccatt cgtcatgctc cataaacgat aaaaccgccc ggaggcgatt
    10741 tcacataaaa cattttgcat cagcgaccaa tcaccacaac ctgaccaccg tccccttcgt
    10801 ctgccgtgct gatctcctga gataccacac gtgaccccac gcgcatttca ccatacagaa
    10861 caggcataac attgccctgg gcaaccatgt tatccagtga ggagaaatag atgttctgct
    10921 taccgttatc cgttgtctgt gtgcggggag ttctggcttt cggtgccagc atctgagcca
    10981 caccgccgag caccatactg gcaccgagag aaaacagaat gccggtcata ccaccggcac
    11041 caatggcagc cccccatgct gcaagggtgg ctccggcggt aaagaatgat ccggcaatgg
    11101 cggctgctcc caggacaatc tggaatacac cacctgactt ggccccggcg actctgggaa
    11161 caatatgaat cacagcgccg tcaggcagag cctcatgtaa ctgcgccgtt aatccggacg
    11221 tgctgacatc ctgcccggca atacgcacct gataccagcc gtcgctcagc ttctgacgaa
    11281 acgcagggag ctgtgtggcc atcgcccgga tggcttcggc acccgttttc acacgaaggt
    11341 cgatgcggcg gccaaatcgt tgcaaatccc cgtaaaggca gatgcgtgcc atgcccggtg
    11401 acgccagagg gagtgtgtgc gtcgctgcca tttgtcggta tacctctctc gtttgctcag
    11461 ttgttcagga atatggtgca gcagctcgcc gtcaccacag taaatggcgg catgattcgg
    11521 caccgatgaa ccaaaacagc acagcagcac atcgcccggc tgcgccgctg acaacggcac
    11581 ctgatacagc cctgtggcct ccagattatc cagatagaga ttctgaccgt gacgccacca
    11641 gtcatcctca cgatgaaaat ccggcatctc aatccccgcc agatggtaag catcccggaa
    11701 cagcgtgtaa cagtccgtca ccccgtgctc aaagcgccgc ccggtaagat gtggcacaca
    11761 gcggaacttg tgaatcgccc cccggcagac cagccaccac ggcaaatcac tctgcacctg
    11821 cagccgccgg tcggcctcac tcagccaggg cagaccaccg gggtggctgt ggaccagtgc
    11881 cacaatctca ccctgcatct ctgcctgcag ccagtcttcc ggcgacatac ggaaatacgc
    11941 ctccggctca ccggagatat tcacgcaggg gaaatatctt tccccctccg gcgttcttac
    12001 cacgaagccg cacgactccg ctggagcaca tcgccgggcg tgcgccagaa tcgctgattc
    12061 tgtctctgtc atgggattta ctgcgaaagt ttgttaatgg aaaggaagcc gccaaagttg
    12121 ccgacgttat tgcgaaactt acagccactc aggcatttgc tgcatttatc cttcgtgatg
    12181 tcggacgttg gctggtcata ttcatccgcg accgccggac cgtgataacc gcactcatcg
    12241 ccgcgatagg tccaggtgca ggtgttggcc agcatgatac gtcccggaaa aacagcgcca
    12301 tctgtttccg tcggcgtgga cagtacaaaa gaggcactca ccgcgctcag ttcgctgcac
    12361 tgctcaatgc gccagcggct gatcacctcc tgctccggat cggcgtcact gtttccgttg
    12421 acgaagttca ccgcatccag aaaacgggcg taaaccttac gccggaccac cgttccgccg
    12481 accagactct gtagatcttc cgccatcccg gtgaccatac cgtacaggtt agaaaccatc
    12541 agcgtggggc gcgtactggt gcctttgcca ttcagttcaa aaccactccc ctgaatggga
    12601 tacggctgat actgtcgccc ctgccaggtg accggctcac ctttttcgtt ctgctcatta
    12661 cagaaaaaat aacgttctcc accgacctct gtcagatcga tttcccagag caccacgctg
    12721 gccgactgct ccgcacgggt gcattcattc agtgtttcct gccggatatc ctgcatcagt
    12781 tcaccacctg ttcaaactct gcgctgaact caacacgcag catactgacc cgcgacgacc
    12841 attttgcgca ggtcaccttt atctgccgcc actcataagg cggcgtccac agaaaggctt
    12901 tccagccccc gtgctcttcc agaaacgact ccagtaccat ggcctcctca cgggggacag
    12961 aaagcgtcac gctgtacgtt ttcaggttgg cattcagccc ggcaggcgct cgctgggaat
    13021 agccatcacc aaagcgcacc tttcttacag aaggggccga agccacatcc ataccgggtt
    13081 tcactttcca gcggaaggtt ttcatcgtcc acctccggag aacaggccac catcacgcat
    13141 ctgtgtctga atttcatcac gggcaccctt gcgggccatg tcatacacgg ccttcatcat
    13201 ctgtggacct ggcagaccat tcgtaccgtc gttctgaatc accacgtgat tgttctgatt
    13261 aaaattaatg ccttcagccc gccgcatctg cgccggactt ccggcaccgc ccacataacc
    13321 accttccgca tagccgcgca tcagccggta gagattcccc acgccaatcc ggctggttgc
    13381 ctccttcgtg aagacaaatt caccacggtg aacaatcccc gctggctcat atttgccgcc
    13441 ggttcccgta aatcccccgg ttgcaaaatg gaatttcgcc gcagcggcct gaatggctgt
    13501 accacctgac gcggatgcgc cgccaccaac agccccgcca atggcgctgc cgatactccc
    13561 gacaatcccc accattgcct gcttaagcag aatttctgtc atcatggaca gcacggagcg
    13621 ggtgaagctg cgccagttct gttcactgcc ggtcagcatc gccgccatat tctgtgcaat
    13681 accatcaaag gtctgcgtgg ctgcactttt aacctgtgac atactgtccg tggcgctctc
    13741 ttcccactca ctccagccgg acttcaggcc tgccatccag ctcccgcgaa gctggtcttc
    13801 agccgcccag gtctttttct gctctgacat gacgttattc agcgccagag gattatcgcc
    13861 atactgttcc ttcaggcgct gttccgtggc ttcccgcgct gcctgccggt cagtcagccc
    13921 ccggtttttc gcatcaatgg ctgcccgttt tgcccgttgc tgctgtgcga atttatccgc
    13981 ctgctgcgcc agcgcattca ggtgctcctg atacgtaacc ttgtcaccaa gtgcagccag
    14041 ctggcgtttg tactccagcg tctcgtcttt atgcgccagc agggatttct cctgtgcgga
    14101 cagctggcga cgttgtgccg cctcctccag taccgcgaac tgactttctg ccttccacaa
    14161 atcccggcgc tgctggctga ttttctcatt cgctccggca tgcttctcca gcgtccggag
    14221 ttctgcctga agcgtcagca gggccgcatg agcactgtct tcctgacgat cgcccgcata
    14281 caccttcacg ccggactgtt tcggcttttt cagcgtcgct tcataatcct ttttcgccgc
    14341 cgccatcagc gtgttgtaat ctgcctgcag gatttttccg tctttcagtg ccttgttcag
    14401 ttcttcctga cgggcggtat atttctccag cggcgtctgc agccgttcgt aagccttctg
    14461 cgcctcttcg gtatatttca gccgtgacgc ttcggtatcg ctctgctgct gcgcattttt
    14521 gtcctgttga cgctgttgat ctaccctctt ttgggctgca tcccgctcca ggcgtttttt
    14581 ttcacgatca tcccagtaac gcgcccgtgc ttcatcgtta acgaaataat catccttgcg
    14641 cagattccag atgtcgtctg ctttcttaaa cgcagcctct gccttaatca gcatctcctg
    14701 cgcggtatca ggacgaccaa tatccagcac cgcatcccac atggatttga atgcacgtgc
    14761 agtcctgtct gcccaggtct ccagcgtgcc catgttctct ttcaggcggc gggtctggtc
    14821 atcaaaccct ttcgttgcgg cctcgttcgc cgcctgcaat gccccggctt catcgccgga
    14881 acgctgcaac tgagcaacat acgcaatctg ctccgccgtc acgttatgga actggcgtgc
    14941 catcgccgtc agccccgacg tcgggtctgt ggtcagcttc ccgaaggctt cagcgacctt
    15001 gtccacctcc acgccggatg cagaggagaa acgcgccaca ctctggctga tggacgcaat
    15061 ctgagcctca ccgcttaccc ccgccttaac cagtgcgctg agtgactcgc tggtctggtt
    15121 aaacgtcagc cctgccgcct gcccggctct ggacaggacc agcatacgat ctgccgtcag
    15181 tcccgcctga ttgccggaaa ggaccagcgt tttgttgaaa tcggacaggg ttgagttgcc
    15241 ctgataccag gcatacgcca gcgcaccggt cgccaccgcc agcgaggtgg cccccaccat
    15301 cggcagggtg atcgcaccgg caagccccct gaacatgggg atcatcccgc cgaaggagtc
    15361 cttaacctga ccaccctgtt gcagcaggat cagccacgga ctttgcccgc ctgcaagctg
    15421 cgtggccacg tcggtgaact gtgcaggcag catacgcatg gcggctttat actgcccgac
    15481 ggaaatcccc gctttctgtg cagccagcgc ctgtcggctc agcgactgtt caacaactgc
    15541 cgctgttttt ttcgcatcac tttccgtacc ggaaaaatga cgcctgactc tggccatctg
    15601 ctcgtcaaat ctggccgcat ccagactcaa atcaacgacc agatcgccta ccggttcagc
    15661 cataccggac tcctcctgcg atcccttctg atactgtcat cagcattacg tcatcctccg
    15721 tcatgtccgc cacatccggg gaagtaggga taacttcatt cccgtccggg ccaaagcgga
    15781 cacctccggc aagccctgcc gctttctgca tcagcacatc atcttcaggc tcttcgtcag
    15841 cctcacgccg gttcagcaga ctgaaatcca gcggatgcat atccggatcg ctgaaaaaca
    15901 ggctgagcac ggtgtacgtc agcccggaaa agtgcatatc cagcagaaca tcatgaaaat
    15961 aatgggtact gtaaaagcgg tgccagtcgg catactccgt ggatgacatc ccggcaagca
    16021 tggcacgcca gtcgggtcgc cccatttcgc gcgccagttt cagggcaaaa ctcagctcac
    16081 cgtcgaacac tttcccgcag aaacaggctc tgcagacccg gcgtcctctg cctgttcagg
    16141 ggcatcattc accacaaact catacatacc ggacagccgg tacaccacgt tttcagcatg
    16201 agaaattgcc tccgtgggcc aggtggtaag cacttcctgc tcaatctgtt taacggcttc
    16261 attcatggaa ggctgctttg tcttctgcgg atggttatgc cacagggaca tcgccaccac
    16321 aaaagcaccg gttctgatgg cgtcttccac agtaaacttc cggttgctgt ctgactccgc
    16381 ctgttctgcc tgccgtttca tcagggcgag atgctcaata cgctgcaggg ctgacagttc
    16441 agaaagcgtg acggtcacac cgttatgttc aaatgattcg gttttcagga acatcgctga
    16501 ctctccggat taactggcgg tgacggtaat ttctgcaacc gcagcaaact caccattacc
    16561 ggatacaacc ggaatgttga ccttgcctgc agcaacacct ttcacggtga tggtcatacc
    16621 actgaccgac acggtggctt ttgttttatc cgctgacacc gcacggaagc tcttgtcggt
    16681 tgcgccttcc ggctggaatg ccacggtcag cgtggtgctc tgccctttca ccaccgaagt
    16741 gctggcaggc gtcacggtca tgccggttgc cgctgttacc gtgctgcgat cttctgccat
    16801 cgacggacgt cccacgttgg tgactttcac cgtacgggtg atcacttcct tcgccgtcac
    16861 cgccttaccg atactgctga cccagccgcg gaacacatcg accgtgccgt tcgggaagcg
    16921 gattttatag gcacgggtat ccccttcatt aaaccacgcc agcagcgcct gctgcccctg
    16981 ctctccgggc atccacgcca gcgtgaagct ggtatctccg gcagatttct gcccctgccc
    17041 ggtcgcagtc cagtccgcat cttcatcatc gagataactg tcgtcatagg actcagcggt
    17101 cagttcgccg ggcgtcaggt ctttaacttt agccagacgc gaccagtcaa cgtctgaaag
    17161 cgggttcgca taagggtcgc cgctcccgtt ataaacccac agggtggttc cggcaccttt
    17221 caccggtatt gctggatttg gtacaggcat atcgtcctca catttcatag gtaatgacat
    17281 acgtcagatc ggctgaactc cacaggcccg catcatcgtc gcgccggtag tcatagccac
    17341 tggccaccat actggtgatc aaatctgaca gtgccgggat atcgctcatc accggataaa
    17401 tccgggactc catccacgca tccagctctg aatccggcac ctgagcaggc aggaaaactt
    17461 cgatatgcag ctccgcctgc caggtatcgc tgtccagctc ttcgcccgtg tattcagcgc
    17521 cggtgagata aacggcaact gccggaaaat ccgcctcatc aaaaacagcg gggcgaccat
    17581 caaaaaacgt cgccccggtg tcatgcttct ccagtgcatc cagtacggct gcacggagtt
    17641 cagtatgttt catcgcttta ttaccatcct cagttgatgc tgcagcgcat agcccagctc
    17701 tttcggaaga cgctcacgcc gtatccgctc aatattctgt ttaaacgccg tggtcagcgg
    17761 caccgccatc ggaattttca ccacatcaat ggggtaacga tttttcccgg ccacacgctg
    17821 cataacatgc cagcggccat ttttcagttg ctgaataaac gcgccgggaa tacgacggtt
    17881 tcccaccaca agcacgctgc cgccaccttt cagggctgaa cgctgcccct ttttacgacg
    17941 cctgcgtcgg ggcaggacaa tccgcgcgtt acccagcttt attacgggca aatccccccg
    18001 gttaaccttg attctggcct gcggattttt gaccgtggcc cttttcagcc tggccctttc
    18061 ctttaccagt ttccggcgta cctttgtctc acgggcaacc tgtgacgccg actgcgatat
    18121 cgcggatgac gcaacgcggt taatggccat tgcggcggca ccgggaaccg ccgttttgct
    18181 gatacggctg aggttttcaa cggcctgctc aagacctttt atgaccatac atcccccttt
    18241 caacgccgac ggttaacggc aggcggaacg ccacgcccaa gccagagatg acagcttccg
    18301 ccatcatccg gcgatacccg gtctatccag aagttttcct caccgatggt cagcgtgtct
    18361 ccacgccgca gctgccgcac ctcatcagtc cggacaaaca gggacgggct ggagccttca
    18421 acgcgcacgc cctgtccggc atagctgata ttttcagggt catcaaaaac accacgtatt
    18481 actgcgccgg actgctcacc ggatgtcatg gtggctgacg ttcccatgta cccgcgtatc
    18541 gtttcatcgg cgcgggcaat ggcagcatcg aacaggttat cgaaatcagc cacagcgcct
    18601 cccgttattg cattctggcc aggccacgtt ctgtcatttc agctgccaca ccggcagaga
    18661 cacggaacgc cgttcccggc agcacaaatg ccacagcctc atcccgcgtg gcgtgaagtg
    18721 catcagtatg cagcgtcacc agtgccacaa ccgtgaccag atcagccgta tcagtcacgg
    18781 tatccggctg cgctgataca acctcatttt catgtccggt cagcgcattt tccgggctga
    18841 cagatgtgtc ctgaccggca gcgtcatccg tgccatcaag ctcctcttcc agctctgcca
    18901 cacggagtgc cagttcttct ttcgtccccg tcaggctgac atcacggttc agttgctcac
    18961 ccagcacctg aagacgggca atcagttcat ctttcgtcat ggactcctcc acagagagaa
    19021 aatggccccg aagggccatg attacgccag ttgtacggac acgaacgcat cagggtcagc
    19081 cagcagcatc agcggtgctg actgaatcat ggtgaactca cgcgccggat cgccggtggt
    19141 cacccagttt ttcgggtagc gggcagaggc gttaatacct tcgcgctgtg cgtccgcatc
    19201 ctgaatgcag ccataggtgc gcagaccgcg tgcctgagtg ttccccagca ccatcgtgtt
    19261 gtccggaagg aagttctttt tgacgtcgtt ttccacatac tgtccggaat acacgacgat
    19321 cgccgtatcg ccatacatcc ccttatagga caccgcttcg cccaggtctt ttaccgctgt
    19381 ctccagctcg gaattagagc cgcgacgggt atccagcttc tccttgacgg ctttgaagga
    19441 acggaacagc gcccagcctt tcggatcaaa cacgatgata ttcaccacac cgctggcgtt
    19501 cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg gacttgtcac gcttgctcca
    19561 ctccgtaccg ccggactgcg tgatgttgtt cgccacactg cggcccatat ccacttcaac
    19621 cggatcgaag gcttcaccgg tcatggtgta tttgccctta agcacggcag aaacggcctg
    19681 catctcttcg acctgagcaa tggccagctc ttcgtctcgc atgttctgca ggatgatgcg
    19741 acggcggcgg taagccgggt ccgccagatt ctgtggatct tcatccggca gacgacgcag
    19801 ggtcatctgc ggattcacct catgcttggg tttgacatat cccggtgtaa attcagaggt
    19861 ggagccgcca cgggagcgga taacctcacc ggaaacaatc ggcgaaacgt acagcgccat
    19921 gtttaccagt cccggaattt gtgagagata gactttctcc gtggtgaagg gatagctttc
    19981 acggaaaaag agacgcagaa acagcggatc aaacttaaat ttctgctcat ttgccgccag
    20041 cagctgggcg gttgtgtaca tcgacataaa aaaatcccgt aaaaaaagcc gcacaggcgg
    20101 cctttagtga tgaagggtca ggttaaacga tgctgattgc cgttccggca aacgcggtcc
    20161 gttttttcgt ctcgtcactg gcagcctccg gccagagcac atcctcataa cggaacgtgc
    20221 cggacttgta gaacgtcagc gtggtgctgg tctggtcagc agcaacagcc agaattccaa
    20281 cggcagcacc gtcggtggtg ccatcccacg caaccagctt acgggtggcg gtgtccagca
    20341 tcagcggagt cattgcaggc gctttcgcac tcaatccgcc gggcgcggtt gccgtatgtg
    20401 ccgggtcact gttgcccagc ggctggtaat gggtaaagat ttctttgctc gtcataaaca
    20461 tcccttacac tggtgtgttc agcaaatcgt taacggcatc agatgccggg ttacctgcag
    20521 ccagcggtgc cggtgcaccc tgcatcagac gatccagcgc agtgtcactg cgcgcctgtg
    20581 cactctgtgg tgctgcggcc agaatgcggc gggccgtttc cacggtcata ccgggggttt
    20641 cggccagcac gcgtgcctgt tcttcgcgtc cgtgagcctc ctcacagttg aggatcccca
    20701 taatgcggct gttttctgcc gcaaccgctg cggtgatctg cgcgttcacg tccggctgcg
    20761 cagcgctggc gttctcgccc tccgtcgctt gcaccacgcc agtaacgtca gcctgcgaag
    20821 cagtggctga aacagttgtt gattgagtct ctttggtcat tcgccctcct gagagacggg
    20881 atttacgtgc atccagtgca tcacgcatga cggtgatcgc atcggtactg ttaacaagtt
    20941 catcagccag tccggcatca atggcctcct gaccgctgta cactgcagcc tcggtatcca
    21001 gcacagcctg cacggacagg ccggtatatg ccgacacctt ctgtgcaaac atccggcggg
    21061 ttgcatccat ccgggactgc agtgtttccc ggacatcatc cggtagatgg ctgtaggggt
    21121 tgccatccac cttatggctg ccgctgtaaa tcagcgtgat ttccacgccc tgtttctcca
    21181 gcgcagcacc gtaattactg tgagccatca tgacgccgat ggagcctgtc cgggcggtct
    21241 gcgtgaccag acgccgggag gcggcactgg caagcagctg acctgcgctg cagttcatgt
    21301 cattggccag cgcccatacc ggctttatgt cacgcacacg ggcgatgatg tcagcgcagt
    21361 caaatgcccc tgccaccatt ccgccgggcg tgtccatatc cagcagaatg ccgtccacca
    21421 tcgggtcgct ggcagcctgt tgcagacggg cgataatgcc gttgtaaccg gtcatccccg
    21481 aatacggctg cagcgcccgc gtccggctga ccagcgtgcc ggaaaccggc agcacggcga
    21541 tgccgttcat gacctgataa ctgcgggcct gtcgtggtcc gtcatcatta acggataacg
    21601 ccagcgtcgc gggtgcctct ccggcagtca ggctgtcacc ggacaccgca tccgtcaggc
    21661 ggctgatccc aagctggcct gcaagcgcac aaaagaaaac ccgcgcatag gcgggttcaa
    21721 gcataagcgg ctcattaaag gccatgctgg caatatgcgg gagattacgc agctctgctg
    21781 tcactcttct cctcctctgt tgattgtcgc agtccggatt caaatgccgc agccgcccag
    21841 gcgggcggtt taagaccggc tgcacggcgc tccatcgttt cacggacctg ctgggcaaaa
    21901 atttcctgat agtcgtcacc gcgttttgcg cactctttct cgtaggtact cagtccggcc
    21961 tctatcagca tcaccgcttc ctgtacttct ttcagaccat cgatggccat acgaccggag
    22021 cctatccagt cacagttccc ccaggcactt cgggcttcct gaaaactgaa gcgcgctttt
    22081 gaaggtaacg tcaccacgcg gcgagcgatg gcctcttcca gccagcacag aaacatctgg
    22141 ctcgcctgac gggatgcgac gaattttcgc cgccccataa agtacgccca cgactcgttc
    22201 gcactggccc gtgccgtgga gtagctcatc tgagcgtaat tccgggaaag ctgctcatac
    22261 gagacaccca gcccggcagc gatataccgc aacagtgact gctcaaacac ggagtagccg
    22321 ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc ccggcatcag gtgcggcact
    22381 tttgcgcctc ccagccggac cggtgctgcg gcgtaatacg cggcaatttc accaatccag
    22441 ccggtcagcc tgtcccgctg ctccttactg ttcgcgccaa gaataaaatc catcgctgac
    22501 tgcgtatcca gctcactctc gatggtggcg gcatacatcg ccttcacaat ggcgctctgc
    22561 agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct ccatcacgct gtaaaacaca
    22621 tttgcaccgc gggtctgccc gtcctccacg ggttcaaaga cgtgaatgaa cgaagcacga
    22681 ccgcccggta actcacgggg tatccatgtc catttctgcg gcatccagcc aggatagccg
    22741 tcctcactga cgtaatatcc cagcgccgca ccgctgtcat taatctgcac accggcacgg
    22801 cagttccggc tgtctccggt attgttcggg ttgctgatgc gcttcgggct gaccatccgg
    22861 aactgtgtcc ggaaaagccg cgacgaactg gtatcccagg tggcctgaac gaacagctca
    22921 ccgttaaagg cgtgcattgc cacaccttcc cgaatcatca tggtaaacgt gcgttttcgc
    22981 tcaacgtcaa tgcagcagca gtcatcctcg gcaaactctt tccatgccgc ttcaacctcg
    23041 cgggaaaagg cacgggcttc ttcctccccg atgcccagat agcgccagct tgggcgatga
    23101 ctgagtcgga aaaaagaccc gacgatatga tcctgatgca gctggatggc gttggcggca
    23161 tagccgttat tgcgtaccag atcgtctgcg cgggcattgc cacgggtaaa gttgggcagc
    23221 agggctgcat ccacactttc actcggtggg ttccacgccc gcaactgccc accaaatccg
    23281 ctgccaccgc cgtgataacc ggcatattca cgcagcgatg tcatgccgtc cggccccaga
    23341 agggtgggaa tggtggacgt tttcatacat aaaatcctgc aggtcccctg cgtcgctgtg
    23401 tcatgccggt ctgcacttcc agctccgcaa tgtatttttt caggtcagac acggaagtgg
    23461 ccgtaaactc cactctccgt ccgtctttct gtaccgttgc cacccgtttt cctgtcatca
    23521 ggtcatgcag tgccgcacgg gcagcggcaa gttcttcctg tcgcgtcatt catcctctcc
    23581 ggataaggca cgggcgtaat ctgccagtg
//
LOCUS       DAZPLM010000045        23515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPLM010000045 DAZPLM010000000
VERSION     DAZPLM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.045"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHVC7_001517"
     CDS             <1..2064
                     /locus_tag="ACHVC7_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR2115606.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHVC7_001518"
     CDS             2080..3099
                     /locus_tag="ACHVC7_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR2115607.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHVC7_001519"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHVC7_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR2115608.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHVC7_001520"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHVC7_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR2115609.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHVC7_001521"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHVC7_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR2115610.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHVC7_001522"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHVC7_001522"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR2115611.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHVC7_001523"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHVC7_001523"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR2115612.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHVC7_001524"
     CDS             6828..7646
                     /locus_tag="ACHVC7_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR2115613.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHVC7_001525"
     CDS             7698..8444
                     /locus_tag="ACHVC7_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR2115614.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHVC7_001526"
     CDS             complement(8418..9383)
                     /locus_tag="ACHVC7_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR2115615.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHVC7_001527"
     CDS             complement(9380..10384)
                     /locus_tag="ACHVC7_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR2115616.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHVC7_001528"
     CDS             complement(10381..11658)
                     /locus_tag="ACHVC7_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR2115617.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHVC7_001529"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHVC7_001529"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR2115618.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="ACHVC7_001530"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="ACHVC7_001530"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR2115619.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHVC7_001531"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHVC7_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="ACHVC7_001532"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="ACHVC7_001532"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR2115620.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="ACHVC7_001533"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="ACHVC7_001533"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR2115621.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="ACHVC7_001534"
     CDS             16207..17562
                     /locus_tag="ACHVC7_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR2115622.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="ACHVC7_001535"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="ACHVC7_001535"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR2115623.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="ACHVC7_001536"
     CDS             18756..19529
                     /locus_tag="ACHVC7_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR2115624.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHVC7_001537"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHVC7_001537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR2115625.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /gene="yegR"
                     /locus_tag="ACHVC7_001538"
     CDS             20916..21233
                     /gene="yegR"
                     /locus_tag="ACHVC7_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR2115626.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHVC7_001539"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHVC7_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR2115627.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="ACHVC7_001540"
     CDS             complement(23071..23403)
                     /locus_tag="ACHVC7_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR2115628.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6062 a         5510 c         6025 g         5918 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcagt
//
LOCUS       DAZPLM010000046        23401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPLM010000046 DAZPLM010000000
VERSION     DAZPLM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.046"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHVC7_001541"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHVC7_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR2115629.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHVC7_001542"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHVC7_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR2115630.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHVC7_001543"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHVC7_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR2115631.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHVC7_001544"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHVC7_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR2115632.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHVC7_001545"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHVC7_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR2115633.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHVC7_001546"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHVC7_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR2115634.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHVC7_001547"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHVC7_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR2115635.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHVC7_001548"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHVC7_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR2115636.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHVC7_001549"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHVC7_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHVC7_001550"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHVC7_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR2115637.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHVC7_001551"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHVC7_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR2115638.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHVC7_001552"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHVC7_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR2115639.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHVC7_001553"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHVC7_001553"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR2115640.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHVC7_001554"
     CDS             complement(14171..14743)
                     /locus_tag="ACHVC7_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR2115641.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHVC7_001555"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHVC7_001555"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR2115642.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHVC7_001556"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHVC7_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR2115643.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHVC7_001557"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHVC7_001557"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR2115644.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHVC7_001558"
     CDS             17912..18760
                     /locus_tag="ACHVC7_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR2115645.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHVC7_001559"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHVC7_001559"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR2115646.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHVC7_001560"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHVC7_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2115647.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHVC7_001561"
     CDS             complement(21672..21872)
                     /locus_tag="ACHVC7_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR2115648.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHVC7_001562"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHVC7_001562"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR2115649.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHVC7_001563"
     CDS             complement(23031..23387)
                     /locus_tag="ACHVC7_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR2115650.1"
                     /translation="MVTLYGIKNCDTVKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5852 a         6224 c         6267 g         5058 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgatggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa cggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 t
//
LOCUS       DAZPLM010000047        23261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPLM010000047 DAZPLM010000000
VERSION     DAZPLM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.047"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="ACHVC7_001564"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="ACHVC7_001564"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR2115651.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="ACHVC7_001565"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="ACHVC7_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR2115652.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="ACHVC7_001566"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="ACHVC7_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR2115653.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="ACHVC7_001567"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="ACHVC7_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR2115654.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="ACHVC7_001568"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="ACHVC7_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR2115655.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="ACHVC7_001569"
     CDS             5281..5739
                     /locus_tag="ACHVC7_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR2115656.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="ACHVC7_001570"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="ACHVC7_001570"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR2115657.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="ACHVC7_001571"
     tRNA            6791..6875
                     /locus_tag="ACHVC7_001571"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="ACHVC7_001572"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="ACHVC7_001572"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="ACHVC7_001573"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="ACHVC7_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR2115658.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="ACHVC7_001574"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="ACHVC7_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR2115659.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="ACHVC7_001575"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="ACHVC7_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR2115660.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="ACHVC7_001576"
     CDS             complement(10157..13900)
                     /locus_tag="ACHVC7_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR2115661.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="ACHVC7_001577"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="ACHVC7_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR2115662.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="ACHVC7_001578"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="ACHVC7_001578"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR2115663.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="ACHVC7_001579"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="ACHVC7_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR2115664.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="ACHVC7_001580"
                     /gene_synonym="iatA"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="ACHVC7_001580"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR2115665.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="ACHVC7_001581"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="ACHVC7_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR2115666.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="ACHVC7_001582"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="ACHVC7_001582"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR2115667.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="ACHVC7_001583"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="ACHVC7_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR2115668.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="ACHVC7_001584"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="ACHVC7_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR2115669.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5836 a         6082 c         5596 g         5747 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcaatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg cgatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat a
//
LOCUS       DAZPLM010000048        23237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPLM010000048 DAZPLM010000000
VERSION     DAZPLM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.048"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHVC7_001585"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHVC7_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR2115670.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHVC7_001586"
     CDS             509..1138
                     /locus_tag="ACHVC7_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR2115671.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHVC7_001587"
     CDS             1165..2826
                     /locus_tag="ACHVC7_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR2115672.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHVC7_001588"
     CDS             3065..3124
                     /locus_tag="ACHVC7_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2115673.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHVC7_001589"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHVC7_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR2115674.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHVC7_001590"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHVC7_001590"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR2115675.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHVC7_001591"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHVC7_001591"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR2115676.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHVC7_001592"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHVC7_001592"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR2115677.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="ACHVC7_001593"
     CDS             9507..10127
                     /locus_tag="ACHVC7_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR2115678.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="ACHVC7_001594"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="ACHVC7_001594"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR2115679.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHVC7_001595"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHVC7_001595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR2115680.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHVC7_001596"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHVC7_001596"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR2115681.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="ACHVC7_001597"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="ACHVC7_001597"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR2115682.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHVC7_001598"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHVC7_001598"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR2115683.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHVC7_001599"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHVC7_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR2115684.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHVC7_001600"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHVC7_001600"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR2115685.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHVC7_001601"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHVC7_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR2115686.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHVC7_001602"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHVC7_001602"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR2115687.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHVC7_001603"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHVC7_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR2115688.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHVC7_001604"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHVC7_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR2115689.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHVC7_001605"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHVC7_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR2115690.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAZPLM010000049        23138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPLM010000049 DAZPLM010000000
VERSION     DAZPLM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.049"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..157
                     /gene="yqiD"
                     /locus_tag="ACHVC7_001606"
     CDS             <1..157
                     /gene="yqiD"
                     /locus_tag="ACHVC7_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR2115691.1"
                     /translation="YWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTRE
                     ETAEKVE"
     gene            complement(215..988)
                     /gene="zupT"
                     /locus_tag="ACHVC7_001607"
     CDS             complement(215..988)
                     /gene="zupT"
                     /locus_tag="ACHVC7_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR2115692.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1131..1919
                     /gene="ygiD"
                     /locus_tag="ACHVC7_001608"
     CDS             1131..1919
                     /gene="ygiD"
                     /locus_tag="ACHVC7_001608"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR2115693.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(1957..3117)
                     /locus_tag="ACHVC7_001609"
     CDS             complement(1957..3117)
                     /locus_tag="ACHVC7_001609"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR2115694.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(3123..3794)
                     /locus_tag="ACHVC7_001610"
     CDS             complement(3123..3794)
                     /locus_tag="ACHVC7_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR2115695.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(3942..5423)
                     /gene="tolC"
                     /locus_tag="ACHVC7_001611"
     CDS             complement(3942..5423)
                     /gene="tolC"
                     /locus_tag="ACHVC7_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR2115696.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            5628..6257
                     /gene="nudF"
                     /locus_tag="ACHVC7_001612"
     CDS             5628..6257
                     /gene="nudF"
                     /locus_tag="ACHVC7_001612"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR2115697.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6258..6680
                     /locus_tag="ACHVC7_001613"
     CDS             6258..6680
                     /locus_tag="ACHVC7_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR2115698.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            6705..7532
                     /gene="cpdA"
                     /locus_tag="ACHVC7_001614"
     CDS             6705..7532
                     /gene="cpdA"
                     /locus_tag="ACHVC7_001614"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR2115699.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            7532..8113
                     /gene="yqiA"
                     /locus_tag="ACHVC7_001615"
     CDS             7532..8113
                     /gene="yqiA"
                     /locus_tag="ACHVC7_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR2115700.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            8142..10034
                     /gene="parE"
                     /locus_tag="ACHVC7_001616"
     CDS             8142..10034
                     /gene="parE"
                     /locus_tag="ACHVC7_001616"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR2115701.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10082..10396)
                     /locus_tag="ACHVC7_001617"
     CDS             complement(10082..10396)
                     /locus_tag="ACHVC7_001617"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR2115702.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(10427..11008)
                     /gene="mdaB"
                     /locus_tag="ACHVC7_001618"
     CDS             complement(10427..11008)
                     /gene="mdaB"
                     /locus_tag="ACHVC7_001618"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR2115703.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(11118..12467)
                     /gene="qseC"
                     /locus_tag="ACHVC7_001619"
     CDS             complement(11118..12467)
                     /gene="qseC"
                     /locus_tag="ACHVC7_001619"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR2115704.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(12464..13123)
                     /gene="qseB"
                     /locus_tag="ACHVC7_001620"
     CDS             complement(12464..13123)
                     /gene="qseB"
                     /locus_tag="ACHVC7_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR2115705.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            13275..13667
                     /gene="ygiW"
                     /locus_tag="ACHVC7_001621"
     CDS             13275..13667
                     /gene="ygiW"
                     /locus_tag="ACHVC7_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR2115706.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            13720..14202
                     /locus_tag="ACHVC7_001622"
     CDS             13720..14202
                     /locus_tag="ACHVC7_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR2115707.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            14748..17006
                     /gene="parC"
                     /locus_tag="ACHVC7_001623"
     CDS             14748..17006
                     /gene="parC"
                     /locus_tag="ACHVC7_001623"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR2115708.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            17239..17976
                     /gene="plsC"
                     /locus_tag="ACHVC7_001624"
                     /gene_synonym="parF"
     CDS             17239..17976
                     /gene="plsC"
                     /locus_tag="ACHVC7_001624"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR2115709.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            18051..19463
                     /gene="ftsP"
                     /locus_tag="ACHVC7_001625"
     CDS             18051..19463
                     /gene="ftsP"
                     /locus_tag="ACHVC7_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR2115710.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            19574..21793
                     /locus_tag="ACHVC7_001626"
     CDS             19574..21793
                     /locus_tag="ACHVC7_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR2115711.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21836..22093)
                     /gene="yqhH"
                     /locus_tag="ACHVC7_001627"
     CDS             complement(21836..22093)
                     /gene="yqhH"
                     /locus_tag="ACHVC7_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR2115712.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22144..23070)
                     /locus_tag="ACHVC7_001628"
     CDS             complement(22144..23070)
                     /locus_tag="ACHVC7_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR2115713.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         5391 a         5872 c         6221 g         5654 t
ORIGIN      
        1 gtactggatt gtattgattg ctctggttgt ggtgggttat ttcctgcatt tgaaacgtta
       61 ttgtcgggcg tttcgccagg acagagacgc actgcttgaa gcgcggaaca aatacttaaa
      121 cagtacgaga gaagagacag ccgaaaaggt agaataaggc tttcgtgaag ctggcagaag
      181 ttgtccatgt gctggacaac ttctgatgtt gtttttaacc aattcccgcc gtttgtagca
      241 gcactaaact gaatcccatc actgacattc cacatagtac gccatagctg gggttattat
      301 tagggtcaat ctctttggcg agcggcatta attcatcaac cgagagcgcc accataattc
      361 ctgcaaccgc cgccatgatt gccgccatga ccaccgggga aatcatgcta ccgaggatta
      421 accacgccag cacaccacca agaatttctg ctagtccaga aatccccgcc cacagaatcg
      481 cggtgcgttt agaccccgtt gccgcataaa ccgggcctgc cactgccaga ccttcaggga
      541 tattgtgcaa cgcgacggcc agtgcgatgc caaatcccag ctccaggttg ctgctcgccg
      601 tgacaaaggt ggcaatccct tccgggaagt tatgcagact gatgccgaga gtgagcagaa
      661 tggctgtgcg cttgatcgat tttggcaacg gctgcaccga tttttgcatt aaatcctgcg
      721 gatgcgcatg tggcaacatg cggtccaggc caaaatagcc aagcagaccg aagataaaca
      781 ttccataacc caacacaggc gacattcctt cagctgctag tgcggcagga agcatttcca
      841 ttaatgagat gagcaacatg atccccgccg caaatcctag cgaaaacgcc agtaagcggt
      901 tcgagggttt ttgcccgaga acgccgagaa acgcgccaat aaacgtggct gcccccgcca
      961 gtatggtcag aatgagaggt actgacatcg acaactcctt ataaatcttc accttcaggc
     1021 agatcgtcaa aataactgat gatcatcatc attgttatcc gggttcttac tccgccgaac
     1081 aagcgtattg tgaggatatc aaaatgacac ctttagttaa ggatatcatc atgtcttcaa
     1141 cacgtatgcc agcattgttt ttaggtcacg gtagtccgat gaacgtgctg gaagataatt
     1201 tgtatacccg cagctggcag aagttgggga tgacattgcc acgcccgcaa gcgattgtgg
     1261 tggtttcggc tcactggttt acccgtggaa caggagtgac cgcgatggag acgccgccca
     1321 cgattcatga ctttggtggc ttcccgcagg cgctgtacga tacgcattat cctgctccgg
     1381 gttcgcctgt gctggcacag catctggttg agctgttagc gtcggtcccg gtgacgctgg
     1441 ataaagaagc ctggggcttt gaccacggtt cctggggcgt gctgattaag atgtatcctg
     1501 acgccgatat cccgatggtg cagttgagta tcgacagtag caaacctgcc gcctggcatt
     1561 tcgaaatggg gcgcaaactg gcagcgctgc gagatgaagg aataatgttg gtcgccagtg
     1621 gcaacgtggt gcataacctg cgtacagtga agtggcacgg tgatagttca ccatatcctt
     1681 gggcgatgtc gtttaatgag tatgtgaaag cgaatctgac gtggcaaggg gcggtagagc
     1741 agcatcctct ggtgaattac cttgaccatg aaggtggcgc gttatcgaac ccaacgccgg
     1801 agcactatct gccgttgttg tatgtcttag gtgcgtggga tgggcaggag ccaattacca
     1861 ttccggtcga tggtatagaa atgggcagcc tgagtatgct gtcggtgcag ataggttaac
     1921 aattaccgga tggcgaatgc catccggcat cattgattac tcaataaaaa tatgtggata
     1981 aaaccgagac atatcctggg tgatcaacgc acggtcttca cgaatgccaa ttccggcagg
     2041 ttgatcgttc accagccagc taccaatcag catatagctg tcgccgaatt tcggtaacgg
     2101 gtggaattgc tgaacaatca tcccttcttc gccatacgga ccttccgctg cttcaatggt
     2161 tttgccgttc tcaatgatcg acacgtttgc gccttcacgg gagaagatcg gtttaaccac
     2221 atatttttcc atttgcggat gatcatcttc cgcaaaataa gcgggcagca ggttcgggtg
     2281 attcgggaac atctcccaca gtagcggtag aagcgctttg ttggagataa tgctcttcca
     2341 cgccggttcc agccagcgta cgcctgcatc ctccagcttg gtggagaaca tctcacgcaa
     2401 cataaattcc catggataca gcttgaacag gttggaaatt acctgatcct gtaaatccgt
     2461 gaactgacct ttttcaccta acccgatatc gtcaatgtag aggaactcgg tagcgatttc
     2521 agcttccgtc gcgcagtcct gcaaatactg aatggttccg cgatcttcca ccgtgtcgcg
     2581 acagcaggtg agatgcagca actggaagcc atactgttca cgcaactcga caaagcgatc
     2641 gattagcttt tcttgcagac tgttaaactg gtcgctgccc tccggcaagt taccggcgtt
     2701 aagctgatct tccagccaga tccactgaaa gaacgccgcc tcgtatagtg acgttggtgt
     2761 atcggcgtta ttttccagca gtttaggttc gccagtgcca tcccatgcca gatcaagacg
     2821 cgaataaagc gatggctggt gagtcagcca tgactgacgc acaaaactcc aggtgcgttt
     2881 tggaatgcgg aatttggtca tcagctcatc gctggcgatc actttttcca ccactttcag
     2941 gcacatctgg tgcagttcgg cggtgacttc ttccagcttt tcaacctggg cgagggtcaa
     3001 cttgtagtaa gcatcttcac accagtacgg ctcgccgtac atggtgtgaa aattgaaacc
     3061 gtattcgtgg gctttttcgc gccagtccgg gcgctcggta atactgactc tttccatcgg
     3121 tatcagccac ccattgaacg agaagaggtg ccggttgcgc tacgctgcat agtgctttgt
     3181 ttggcaacag attcaccaaa accgccacgg gtaacggtag tagtggtcgc cggttttggt
     3241 gccatggctg tcttcggtac ggtcatggtg cggcctggct gggctgcgcc atagttttta
     3301 cccgtcgcgt cggtatattt accgtaagcc gggctggctg ggtttttcga ggagaacagc
     3361 ggctgctgtg caaatcccgc gccgccgccc atcagacgcc ccatcatgta accggccatc
     3421 agcggcatcc agaaactccc gctggattgc tgggcctgcg cctggttttc tggtgccatg
     3481 ccagcctggg ctggtgcctg ctggcactga ccttcaccaa attcagcaac acagtcttca
     3541 cgggtggcgt atttcggcgc agtacgttcg gcttctttca gcgcattgtt gtacgcggtg
     3601 gtacattcgg cgcttttgcc tgggtttgca gctgaacagt cgtcagcatt ttgatagaga
     3661 gacactgttt catcactctt ttcacagcca gccagcataa aaacagtggc aaccgcgaga
     3721 gcgactggtg tcagatggcg tgcgctccag tttttgcgga acgatgcgtg gcgtatggat
     3781 tttgtccgtt tcatttttgt cttccgggac cagtggtaaa tacccatcag aatagaggat
     3841 ggctggtcga aattgaagcg agaaggggga agaatgcggc agataacccg tatctttacg
     3901 ttgccttacg ttcagacggg gccgaagccc cgtcgtcgtc gtcagttacg gaaagggtta
     3961 tgaccgttac tggtggtagt gcgtgcggat gtttgctgaa cgactggtgc cgggctatca
     4021 ggcgcataac catcagcaat agcattctgt tccggcgttt gcggtgcaac gttttccgga
     4081 ttagtggaaa ccggtttgct cagcgcattg ttcagtgcca gcagatcctg ctcgttcaac
     4141 gtacccagag ctgacttaat attcaactgg ttaatcaggt agttataacg cgcatttgcc
     4201 agctcttgct tagcgttgta cagcgtggtg gttgcatcca acacatcaac aatggtacgc
     4261 gtaccgaccg agtagcccgc ttccatcgcg tctaatgagc tttgagcgga aactacggct
     4321 tgtttgtagg cgttaatgct actgatagat gcattaatgt tgttgaagga ggaacgtacg
     4381 gtttgcacga cgctacgatg cgcgctttcc agttgctcgc tggcaccaac aaagttgtac
     4441 tgggcctgtt tcacctgcga gttaaccatt ccgccctgat aaatcggcag cgagaagctc
     4501 aggcccactt tgttctggcc catattgctg tcgtcatact gggtaccagc ggcaccacgg
     4561 gttttcgaac cgctataaga ggtgtcagaa atcccggtag aagccgttaa atccagcgtc
     4621 ggtaagtgac catcctgcgc ctggcgaatt tgctcgcgcg ccaggtcctg gctcaagcgt
     4681 gcctgtaaca gcgacaggtt gcgtttttcg gcttctttca gcagcgcgtt aaccggctgt
     4741 ggtttgtcgg ttttaaagtt ttcgacattc agcgccgcca gttccggata gtagttaccg
     4801 gtgatctggc gcagctgctc taccgcgtta tcaaggttat tacgtgcggt cacttcgttc
     4861 gccagcacgg tatcgtactg cgcgcgggcg ttctgcacgt cggtgatcgc taccaggccc
     4921 acgttaaaac gttgggtggt ttgatctaat tgacggtaga tcgcttcttt ttgtgcctgt
     4981 gtataggaaa gaacgtcaat agcattcaac acgttgaaat aagcggtcgc ggtgttgagg
     5041 atcaaggttt gctgatcggt ctgatatgtg acgtcctgaa tccctgctgc tttttcctgc
     5101 agcgttaacg cacgccattt cgacatatca aaaatggatt gagttaactg cagggacgca
     5161 ctggtcgcgt tagagttgat gccgttcgcg tcgcggtagc cgttgctata ggtgtaatct
     5221 gcacctaaac ctagctgtgg cagtaatgga ctgcgcgctt cattaatttt ttcaaaggca
     5281 gcatcacgat cggcggcaga cttacgcaat tccgggttac taaggcgtgc ttgctgataa
     5341 acttgcatca ggttctcggc ctggctcaac gaactgaacc cagaaaggct caggccgata
     5401 agaatgggga gcaatttctt catttgcatt ccttgtggtg aagcagtatt tagcgcgatc
     5461 aaactgtaaa attattgcgg attctagcag aagccgctac cgcaaaaggt tggcgttacg
     5521 tgccataggg cgttaatgtg cctcaattta acacatgact gctcaaacgg cagtcaaacg
     5581 tcgctgaaat tcacatttaa ttcactatta gtgccaggac attaacaatg cttaagccag
     5641 acaacctgcc cgttacattt ggcaaaaacg atgtagaaat tattgcacga gaaacacttt
     5701 atcgcggctt tttttcatta gatctttata gatttcgtca tcgtctattc aacgggcaaa
     5761 tgagtcatga ggtacggcgg gaaatttttg agcgcggtca cgccgcagtc ttactaccct
     5821 ttgacccagt gcgcgatgaa gttgtgctga ttgagcagat tcggattgcc gcatacgaca
     5881 ccagtgaaac cccctggcta ctggagatgg ttgccgggat gatagaagag ggtgaaagtg
     5941 tggaagatgt tgcccgtcgc gaagcgattg aagaggcggg actgatagtc aaacggacca
     6001 aaccggtgtt aagtttcctg gcaagcccgg ggggcaccag tgagcgttcg tcaattatgg
     6061 tgggcgaagt ggacgccacg accgcaagcg gtattcatgg tctggctgat gaaaacgaag
     6121 atattcgcgt tcatgtggta agccgggaac aggcatacca gtgggtagaa gaggggaaaa
     6181 tcgacaacgc agcgtcggtc atcgctttgc aatggctgca gctgcatcat caagcgttaa
     6241 aaaatgagtg ggcataaatg aagcgttaca cacctgactt tcctgaaatg atgcgcctgt
     6301 gcgagatgaa cttttcacaa ttgcgccgtt tgttaccgcg caatgacgca cccggcgaaa
     6361 ctgtaagcta tcaggtggca aacgcacaat atcggctgac gattgtggaa tcgacccgat
     6421 acactaccct ggtgacaata gaacagactg cgcccgcgat cagttactgg agccttccgt
     6481 caatgacggt gcgtctgtat catgacgcga tggtggctga agtgtgttca agccagcaga
     6541 tttttcgctt caaagcgcgg tatgattatc ctaataaaaa gttgcatcaa cgcgacgaaa
     6601 agcatcaaat taatcagttt ttagcggact ggttgcgata ctgtttagca catggagcga
     6661 tggcgattcc ggtttactag catcgtgaaa cctaaggaca ccatttggaa agcctgttaa
     6721 cccttcctct ggctggtgag gccagagtca ggattttaca aattaccgac actcacctgt
     6781 ttgcacaaaa gcacgaagct ctgttagggg taaacacctg ggaaagctac caggcggtgc
     6841 tggaggcgat tcgtccacat cagcacgaat tcgacctgat tgtcgcgaca ggtgatttag
     6901 cgcaggatca atcctctgcg gcctatcagc atttcgctga aggcatcgca agttttcgtg
     6961 cgccctgcgt ctggctgccg ggcaaccacg atttccagcc cgcgatgtac agcgcgttac
     7021 aggatgcggg tatctccccg gcgaagcggg tgtttattgg tgagcaatgg caaatcctgt
     7081 tgctggatag ccaggtgttt ggcgtgccgc acggtgagct gagcgagttt cagcttgagt
     7141 ggctggaacg taaactggcc gatgcgccag aacgccatac gttgctgctg ctgcatcatc
     7201 atccgctacc tgcgggttgt agttggctcg atcaacacag tctgcgtaac gcgggcgaac
     7261 tggataccgt gctggcgaag tttccgcacg tcaaatactt gctgtgcggt catattcatc
     7321 aggagctgga tctcgactgg aacggtcgcc gcctgctggc aacgccgtcg acctgtgtgc
     7381 agtttaagcc gcactgttcc aactttacgc tggatactat cgcgcccggc tggcgtactc
     7441 tcgagttaca tgctgatggc acgctgacca ccgaggtgca tcgcctggcg gacacacgtt
     7501 tccaacctga taccgcttca gaaggctact gatgtctacg cttctttatt tacacggttt
     7561 caacagctcg ccgcgctctg caaaagcgag cttgttaaaa aactggctgg cggaacatca
     7621 ccctgacgtt gagatgatca ttccgcagtt gccgccgtat ccttccgacg cggcagaact
     7681 gctggaatcc attgtcctgg aacatggcgg cgattcgctg ggtattgtcg gttcgtcact
     7741 ggggggatat tacgccacct ggttgtcaca atgttttatg ctgcccgcag tggtggtaaa
     7801 cccggcggtg cgcccgtttg aactgctgac ggactatctc ggtcagaacg agaacccata
     7861 caccgggcag caatatgtgc tagagtcacg ccatatttac gatctcaaag tcatgcagat
     7921 tgacccgctg gaagcgccgg atttgatctg gctgctgcaa cagacgggag atgaagtgct
     7981 ggattaccgc caggcggtgg cgtactacgc ttcctgccgc cagactgtca tagaaggcgg
     8041 caaccacgca ttcacgggct tcgaagatta tttcaacccg atcgtcgatt ttcttggtct
     8101 gcaccatctc tgacgatcaa ctcgaattgc taacttaaac catgacgcaa acttataacg
     8161 ctgatgccat tgaggtactc accgggcttg agccggttcg ccgccgtccg gggatgtata
     8221 ccgataccac tcgccctaac catttggggc aagaagttat tgataacagt gtcgatgaag
     8281 cactggcggg tcacgcaaaa cgcgtggatg taatcttaca tgctgaccag tcgttagaag
     8341 ttattgacga tgggcgcggg atgccggtgg atattcaccc ggaagagggt gtcccggcgg
     8401 ttgaattgat tctttgccgt ctgcatgcag gcggtaaatt ctctaacaaa aattaccagt
     8461 tctctggcgg cctgcatggc gtggggattt cggtggttaa cgccctgtcg aagcgcgttg
     8521 aagttaacgt gcgccgcgat ggtcaggttt ataacatcgc ctttgaaaat ggcgaaaagg
     8581 tgcaggattt acaggttgtc ggcacttgcg gtaaacgcaa taccggtacc agcgtgcact
     8641 tctggccgga tgaaaccttc tttgacagcc cgcgattttc tgtttcacgc ctgacgcatg
     8701 tgctgaaagc caaagcggta ctgtgccccg gcgttgagat tacctttaaa gatgagatca
     8761 acaacaccga acagcgctgg tgctatcagg acggtctgaa tgattacctg gcggaagcgg
     8821 taaacggttt accgacgctg ccagaaaaac cgtttatcgg taatttcgct ggcgatactg
     8881 aagctgtgga ctgggcgcta ctgtggctgc cggaaggcgg tgaactgctg accgaaagct
     8941 acgtcaacct gatcccaacg atgcagggcg gtacccatgt taatggcctg cgtcagggcc
     9001 tgttggacgc gatgcgtgag ttctgtgaat atcgcaacat tctgccgcgc ggcgtgaagc
     9061 tgtcggcgga agatatctgg gatcgctgcg cctatgttct gtcggtgaaa atgcaggatc
     9121 cgcagtttgc cgggcagacg aaagagcgtc tctcttcgcg tcaatgcgcg gcattcgttt
     9181 ctggcgtggt gaaagatgcc tttatcctgt ggctgaacca gaacgttcag gcggcggagt
     9241 tgctggcaga gatggcgatt tccagcgccc agcgtcgtat gcgtgcggct aaaaaagtgg
     9301 tgcgtaaaaa gctgaccagc ggcccggcgc tgcctggcaa actggctgat tgtaccgcgc
     9361 aggaccttaa ccgtaccgaa ctgttcctcg tggaaggtga ctccgcaggc ggatctgcca
     9421 agcaggcgcg cgatcgtgaa tatcaggcga tcatgccgct gaaaggtaag atccttaaca
     9481 cctgggaagt ttcttccgac gaagtgctgg cctcgcagga agtgcatgat atttcggtag
     9541 cgatcggtat cgatcctgac agcgacgatc tgagccagct tcgttatggc aaaatctgta
     9601 tcctcgcgga tgcggactct gatggtctgc acattgccac gctgctctgc gctttgttcg
     9661 taaaacactt ccgcgcgttg gtgaaacacg gtcacgttta cgtcgcactg ccaccgctct
     9721 accgtattga tctcgggaaa gaggtttatt acgcgctgac ggaagaagag aaagagggcg
     9781 tacttgagca attaaaacgc aagaaaggca agccgaacgt ccagcgtttt aaaggtctgg
     9841 gggaaatgaa cccgatgcaa ttgcgtgaaa ccacgcttga tccgaacact cgccgtctgg
     9901 tgcagttgac tatcgatgat gaagacgatc agcgtactga cgcgatgatg gatatgctgc
     9961 tggcgaagaa acgctcggaa gatcgccgca actggttgca agagaaaggc gacatggcgg
    10021 agattgaggt ttaaaggaaa gaacattgcc cggtcaggat ggccgggcaa acaaggcagg
    10081 attaaatccc tggctgcaga atacgaatat tcatctccag cacgtcgcct tttacggctt
    10141 cgctgtacgc cttcatgtgc ggggtttgca gatgcgcttc aaggtgcgcg atgctttccc
    10201 actgctcaat catcacgata gaatccggtg ccatagactg gaaactcacg ccagcagcgc
    10261 aatccaccat tggcgcatag ccgtggcaac cttcttcttt cagtacggtt ggaacgattt
    10321 tagcaaactg atccaatacc gcctgacggt gatgttgacc aggacgagta cggatttctg
    10381 cgattacggt aagcatggtt aactccttct aaagcctgag ctctagttaa ccaaaaattt
    10441 ccacaagatg cttgcgatat tcttcagtat agcggggaac atcaggcatt tttatcacgt
    10501 cattagcgat aaatgtcggc agcggttcca tgccaaggaa ttggtttgct ttatggaacg
    10561 gcagatacac accgtcgacg ccaacgccgt ggaagaattg atctttttcg gtgaaggctt
    10621 ccattggtgc gttccaggtc agagaaagca tatatttttt gccctgtacc aggccgccgg
    10681 aaccgtattt ttttgacgga tctttgcggg tacggccatc gctggcatac agcgtcccgt
    10741 gaccttcggt gaatacatca tcaatgtatt ttttcactgt ccacggcgcg cccatccacc
    10801 agcctggcat ctgccagatc accacatcag cccagagaaa gttttgtact tccgctttga
    10861 catcgtagtc gctgtcggcg cgaacgatgc gaacatcatg cccgaggtcg cgcagtgtgc
    10921 catccgcgac ttcggtcagg gtgtcgttca gttgaccatt ggagtgggcg aattttttcg
    10981 cgccgttgat aatcagggtg ttgctcattt tttaacctca aggtgagacg tttgtcgact
    11041 atagtacgtc acagagcggt acggtaaaat tagcaaaatg tgcaaagtct tttgcgaatt
    11101 tagcaaaagt cttaccctta ccagctcacc ttcgcctcaa atccaccttg ttccgcattc
    11161 ccaaattcaa cattcatgcc atgcaatttg gcgattcgct ggacaatcga tagcccaagt
    11221 ccactgccgg tagcggtttg tccggggggg cgatagaagc gttcgccaat tcgcgccagt
    11281 gcctctggtg tcacaccggg gccgttatcc ctcacgatga aattatcggc attcagcgtg
    11341 acgtccacca cgctgccctg tggactgtag cgcacggcgt tatccagaag gtttcgcacc
    11401 aacaaactta gcaatagcgg ttgtccagtg cgtttgatgc tgtgggcatt gagtgtcagt
    11461 cgcacgtcaa ttttcgcctg ctgcgccgtg tggtaaatat ccatcaccga cgattgcagg
    11521 agatcttcaa gcgggatctc cgcgacgtcc tgaaggttat ccagtgagtc cagccgcgat
    11581 agcgtgagca gttgatcaac cagacgagta gcgcgatcga tcccggaatg taattggagc
    11641 agtgcttttt tccgcgcctg cggatcatcg tcagagagct gcgcaacttc ggtttgcact
    11701 ttcagcgctg ttaacgggct acgtagttcg tgagctgcat cggaggtaaa gcgtcgttct
    11761 cgaaccatca tcgcatgtgt gcgggcgaac agttgattta gcgactcaac cagtggtcgc
    11821 acttcgctgg gtacgccagt cgcgtttagt ggtttttccg agtcagggtc acgcatacgt
    11881 agtgccagcg ccagtttgtt cagcggcgcg agttcacgac ccagtagtac catcatgatg
    11941 attaacataa tcggcagtgc gaccagccac gggatcaatt gcccggcaac aatcgccagc
    12001 gccatgtctt cacggtattc ccattcctgg ccaacaacga tgcgatattt gccatcaggt
    12061 gaggtcatcc agacaaaacg ccaaggatcg tcttcaccga ccagttgccc gtcagcaaaa
    12121 ccttcccgtt gatagctata gggaatatct tctccgttat cgccatcatt aaggaccatt
    12181 ctgccgtcgt gggtaaagat ggcaaaggtc agcgcatcgt catcaacatg accgtgtttt
    12241 aatttatttg gcgtctgtgc catgcgatcc gccgcgttga tttcgttgag atcgagcgta
    12301 cttaaccgct tggcaaacag catcagttgg gtgtcgaaca attcatcgac gttatccgtt
    12361 gtttgtttcc aggcgacaaa gctggaaagc agccaggtca ccgaggccag aattaaaaag
    12421 attagcgtca gcctgacgcg cagactaaga cgttgggtaa atttcatttc tcacctaatg
    12481 tgtagccaat accatgcacg gtacgaataa aatcactacc gagtttgcgt cgcagatgat
    12541 gcacatgcac ttcaacggca ttactggtga cctcttcgtc ccaggtatac agtttctctt
    12601 caatcagttt gcgcggcagt acccgaccag cgttacgcat cagtaattcc agcagggcaa
    12661 attcttttgg tttcagtgtt aagggttcgc cagccagcgt ggcgatacgt ttgccggggt
    12721 cgagcatgac gttaccgtgg cgcagctcgt tgctggcctg gccgttggtt cggcgcatca
    12781 gagcctccag cctggcggcg acttctatca acgcaaaagg tttacacaga taatcgtcag
    12841 ctcccagacg cagcccttct acacgttccg ctaacgcatc acgcgcggtc aggatcagta
    12901 ccggctcacg ctgacctttc tctcgccatt cgcgcaaaat atcgcgacca tccatgcctg
    12961 gtaaggttaa atccaggatc accgcatcat aaggtgcgct ataaagcgcc tcttttccct
    13021 gacgaccttg tgtaaaccag tcgacactaa aacccatttt actaaggcca gttttgatgc
    13081 cgtcgccaat cagcatgtca tcttctatca gtaaaattcg cattttttca tccctgcgat
    13141 aaccgtattt gctgccagta aaccgtgttg taacgcgctc tgtacagcat taaattttat
    13201 ttttttccct taagaagttg ttaaggacta tcttgttaaa tgctcggaaa cagacattaa
    13261 agggagtaat aaacatgaaa aaattcgcag cagtaatcgc agtaatggcc ctgtgcagtg
    13321 caccggtgat ggcagcagag cagggcggtt tttctggccc atcggcaacg caaagtcagg
    13381 ccggaggatt ccaggggccg aacggcagcg taacgactgt agaaagcgca aaatccctgc
    13441 gtgacgacac ctgggtaacc ctgcgcggca atatcgttga acgcatctct gacgatctct
    13501 acgtgtttaa agatgccagc ggtactatca atgttgatat cgaccacaaa cgctggaacg
    13561 gtgtgacggt gacgccgaaa gatacggttg agattcaggg tgaagtcgat aaagactgga
    13621 attctgttga aattgacgtc aaacagatcc gcaaagtaaa tccgtaattg ttaccgctcc
    13681 cgggacgcgt tcccgggaat aatttcgcag ggaggcaaaa tgacaaacct gacactggat
    13741 gtaaacatta tcgatttccc atcaatccct gtggcgatgt tgccgcaccg ctgtagccct
    13801 gagttgctca actacagtgt ggcgaaattt atcatgtggc gtaaagagac ggggctttct
    13861 cctgttaacc aaagccagac ttttggcgtc gcctgggacg accctgccac caccgcaccg
    13921 gaagcgtttc gctttgatat ctgcggcagc gttagcgaac cgattcccga taatcgttat
    13981 ggtgtgagca atggtgaact taccggtgga cgttatgccg tggcccgcca cgttggcgag
    14041 ctggacgata tttcacacac gatatggggc attattcgcc actggctgcc tgcaagcggc
    14101 gagaaaatgc gtaaagcacc gattctgttt cactacacca atcttgccga aggggtgacg
    14161 gagcagcgac tggaaacgga tgtttatgtg ccgttagcgt gatggggaat ctttaccgga
    14221 gtgtgagcca aatcactcgc gataagtttg agcgaacaat gccacaggcg tattgttgtt
    14281 gggcttataa tttctccggt aaggaatacc atgtatacgc gaaatttatt atccggtgcg
    14341 ccgtaagccg tctcgggggt acccatgagc aagccgcact tatggcagga ggcgcggggc
    14401 actggtggag acacagctaa actgggctga accatatact tacagtcgac tactttgatg
    14461 ctttaggctt ccctggactc gcataacctc aacttctcga atcgcccgga gcggaccccc
    14521 atgccgagtg gtgtggcatg ggcgcaacct tgtgctgccc tctatgccga tcaagtctac
    14581 agcaaagagt tgtatatcaa ggcacattga tgcctttatc gccgtcggag cacgtccggc
    14641 ggcgctattc acgctgatct cctgtgactc gacgcggcag ataatgtagt atctccggca
    14701 atattgcccc tttgaaggct ggcgaatcag ttgaggaatc agaattaatg agcgatatgg
    14761 cagagcgcct tgcgctacat gaatttacgg aaaacgccta cttaaactac tccatgtacg
    14821 tgatcatgga ccgtgcgttg ccgtttattg gtgatggtct gaaacctgtt cagcgccgca
    14881 ttgtgtatgc gatgtctgaa ctgggcctga atgccagcgc caaatttaaa aaatcggccc
    14941 gtaccgtcgg tgacgtactg ggtaaatacc atccgcacgg cgatagcgcc tgttatgaag
    15001 cgatggtcct gatggcgcag ccgttctctt accgttatcc gctggttgat ggtcagggga
    15061 actggggcgc gccggacgat ccgaaatcgt tcgcggcaat gcgttacacc gaatcccggt
    15121 tgtcgaaata ttccgagctg ctattgagcg agctggggca ggggacggct gactgggtgc
    15181 caaacttcga cggcactttg caggagccga aaatgctgcc tgctcgtctg ccaaacattt
    15241 tgcttaacgg caccaccggt attgccgtcg gcatggcgac cgatattcca ccgcataacc
    15301 tgcgtgaagt agctcaggcg gcaatcgcat taatcgacca gccgaaaacc acgctcgatc
    15361 agctgctgga tatcgtgcag gggccggatt atccgactga agcggaaatt atcacttcgc
    15421 gcgccgagat ccgtaaaatc tacgagaacg gacgtggttc agtgcgtatg cgcgcggtgt
    15481 ggaagaaaga agatggcgcg gtggttatca gcgcattacc gcatcaggtt tcaggtgcgc
    15541 gtgtactgga gcaaattgct gcgcaaatgc gcaacaaaaa gctgccgatg gttgacgatc
    15601 tgcgcgatga atctgaccac gagaacccga cccgtctggt gattgtgccg cgttccaacc
    15661 gcgtggatat ggatcaggtg atgaaccacc tcttcgctac caccgatctg gaaaagagct
    15721 atcgcattaa cctcaatatg atcggtctgg atggtcgtcc ggcggtgaaa aacctgctgg
    15781 aaatcctctc cgaatggctg gtgttccgtc gcgataccgt gcgccgccga ctgaactatc
    15841 gtctggagaa agtcctcaag cgcctgcata tcctggaagg tttgctggtg gcgtttctca
    15901 acatcgacga agtgattgag atcattcgta acgaagatga accgaaaccg gcgctgatgt
    15961 cgcggtttgg ccttacggaa acccaggcgg aagcgatcct cgaactgaaa ctgcgtcatc
    16021 ttgccaaact ggaagagatg aagattcgcg gtgagcagag tgagctggaa aaagagcgcg
    16081 accagttgca gggcattttg gcttccgagc gtaaaatgaa taacctgctg aagaaagaac
    16141 tgcaggcaga cgcgcaagcc tacggtgacg atcgtcgttc gccgttgcag gaacgcgaag
    16201 aagcgaaagc gatgagcgag cacgacatgc tgccgtctga acctgtcacc attgtgctgt
    16261 cgcagatggg ctgggtacgc agcgctaaag gccatgatat cgacgcgccg ggcctgaatt
    16321 ataaagcggg tgatagcttc aaagcggcgg tgaaaggtaa gagcaaccaa tcggtagtgt
    16381 ttgttgattc caccggtcgt agctatgcca tcgacccgat tacgctgccg tcggcgcgtg
    16441 gtcagggcga accactcacc ggcaaattaa cgttgccgcc tggggcgacc gttgaccata
    16501 tgctgatgga aagcgacgat cagaaactgc tgatggcttc cgatgcgggt tacggtttcg
    16561 tctgcacctt taacgatctg gtggcgcgta accgtgcagg taaggctttg atcaccttac
    16621 cggaaaatgc ccatgttatg ccgccggtgg tgattgaaga tgcttccgat atgctgctgg
    16681 caatcactca ggcaggccgt atgttgatgt tcccggtaag cgatctgccg cagctgtcga
    16741 agggcaaagg caacaagatt atcaacattc catcggcaga agccgcgcgt ggagaagatg
    16801 gtctggcgca attgtacgtt ctgccgccgc aaagcacgct gaccattcat gttgggaaac
    16861 gcaaaattaa actgcgcccg gaagagttac agaaagtcac tggtgaacgt ggacgccgcg
    16921 gtacgttgat gcgcggtttg cagcgtatcg atcgtgttga gatcgactct cctcgccgtg
    16981 ccagcaacgg tgatagcgaa gagtaatatg ccggggaggg cctgtttcct cccgcttgca
    17041 aggaacgccg gatgaaacgt taagtcgtca tccggcagcg tattcaaccc ccggaaacga
    17101 taaaaattcc cttgaaaccg ttgtttattc atgcgttgcg attaacaata cgcttttcca
    17161 gagagcggct tttaacaatg ccctaacctg atttcaggtg acgtacaatg ccagtctctc
    17221 agaccttcag agggtgttat gctatatatc tttcgtctta ttattaccgt gatttacagc
    17281 atcttagtct gtgtattcgg ctccatttac tgccttttca gcccgcgtaa cccgaaacat
    17341 gtggccacct ttgggcatat gtttggccgt cttgcgccgc tgttcggcct gaaagttgag
    17401 tgccgcaaac ctgcagatgc tgaaagctac ggcaatgcta tctatatcgc taaccaccag
    17461 aacaactatg acatggtgac agcatcgaac attgtgcaac cgccgacggt gacggtaggt
    17521 aaaaagagct tgctgtggat ccccttcttc gggcagttgt actggttaac cggcaactta
    17581 ttgatcgaca gaaacaatcg cactaaagct cacggcacca ttgcggaagt cgtgaatcat
    17641 ttcaaaaaac gccgtatttc catctggatg ttcccggaag gaacccgcag ccgtggtcgc
    17701 ggcctgctac cgttcaagac tggagcattt cacgcggcaa ttgcggcggg cgtcccgatt
    17761 attcccgtgt gcgtctctac aacttcgaat aagattaatc ttaatcgact gcacaacggt
    17821 ctggtgattg tcgaaatgct gccgccaatt gacgtcagtc agtatggcaa agatcaggtt
    17881 cgtgagctgg ctgcccattg tcgttcgata atggaacaaa aaatcgccga gctcgataaa
    17941 gaagtcgcag aacgcgaagc cgccggaaaa gtttaagtcg gcaatctgta tttttgcggg
    18001 gaacactttc ctgcacggta ttactttagc cagttttaca tggagcaaat atgtcactca
    18061 gtcggcgtca gttcattcag gcatcgggga ttgcactttg tgcaggcgct gttcccctga
    18121 aggccagcgc agccgggcaa cagcaaccgc tacccgttcc gccgctactt gaatctcgcc
    18181 gtgggcaacc gctgtttatg actgtacaac gtgcgcactg gtcatttacg ccagggacac
    18241 gcgcgtcggt ctggggaatc aatggtcgtt acctggggcc gactatccgc gtctggaagg
    18301 gcgacgatgt taagcttatt tacagcaacc gcctgacaga aaatgtctca atgacggtgg
    18361 ccgggctaca ggtacctggc ccgctgatgg gcggtccggc acggatgatg tcgccaaacg
    18421 ctgactgggc acccgtactg cctattcgcc agaacgcagc tactctgtgg tatcacgcca
    18481 atactcccaa ccgcacggct cagcaggtct ataacggcct tgccggaatg tggctggtgg
    18541 aagatgaagt cagcaagtcg ctgcctatcc ccaaccatta tgatgtggat gattttccgg
    18601 tcattatcca ggataaacgg ctggataact ttggtacgcc agaatacaac gaaccgggaa
    18661 gcggcggctt tgttggtgat acgctgctgg ttaacggtgt acaaagcccg tacgttgaag
    18721 tctcgcgtgg ctgggtgcgc ttgcgactgc tgaacgcgtc gaactctcgt cgctatcaac
    18781 tacagatgag cgatggtcgc ccgttacatg tgatttctgg cgatcaggga ttcctgcctg
    18841 ctcctgtatc ggtgaagcaa ctttcgctgg caccgggcga gcgccgcgag attctggtgg
    18901 atatgagcaa cggcgatgaa gtgtcgatca cctgtggcga agcggcgagc attgttgatc
    18961 gtattcgcgg cttctttgag ccatccagta ttctggtttc taccctggtg ctaacgctgc
    19021 gcccaaccgg ccttctgccg ctggtcacag acagtcttcc gatgcgcttg ctgccaactg
    19081 aaatcatggc cggttcgccg attcgcagtc gcgatatcag tctgggtgat gacccgggta
    19141 ttaatggaca gctgtgggac gtcaaccgta ttgatgtcac cgcgcagcaa ggaacgtggg
    19201 aacgctggac ggtacgcgcg gacgagccgc aagcgttcca tattgaaggc gtgatgttcc
    19261 agatccgtaa cgtgaatggt gcgatgccgt tcccggaaga cagaggctgg aaagataccg
    19321 tttgggttga cggacaagtg gagctgcttg tttatttcgg tcagccttcc tgggcgcact
    19381 tcccgttcta cttcaacagt cagacgctgg aaatggcgga ccgtggctcg attgggcaac
    19441 tgttagtcaa tccggtaccg taatcttcat actcgcccga ggagggcgag tatggaatac
    19501 ttttcgattt ctctttcgtt ccggcttata atccccggcc tttgattatt tttttatcat
    19561 ttctggaagc aaaatgagct ctatctccct gatccaaccg gatcgcgacc tgttctcctg
    19621 gccgcagtac tgggccgcct gttttggacc ggcaccgttt ttgccgatgt ctcgtgaaga
    19681 gatggatcaa cttggctggg atagctgcga catcattttg gttactggcg acgcgtatgt
    19741 cgatcaccca agcttcggga tggcgatttg cggtcgtatg ctggaagcac agggctttcg
    19801 cgtcgggatc atcgcccagc cggactggag cagcaaagac gactttatgc gtctgggtaa
    19861 accgaatctg tttttcggtg ttactgctgg caacatggat tcgatgatca accgttatac
    19921 cgccgatcgc cgtttacgtc atgacgatgc ctacacgccg gataacgtcg cgggtaagcg
    19981 cccggatcgc gccacactgg tttataccca gcgttgtaaa gaggcgtgga aagatgtacc
    20041 ggtgatcctc ggcggtattg aggctagtct gcgccgtacc gcacattatg attactggtc
    20101 cgataccgtg cgccgttccg tgctggtgga ttcgaaagcc gacatgctga tgtttggtaa
    20161 cggtgagcgt ccgctggtgg aggtggcgca ccgtctggcg atgggcgagc cgattagtga
    20221 aatccgcgat gtgcgtaata ccgcgattat cgtgaaagag gcgctgcctg gctggagcgg
    20281 cgtggattcc acccgtcttg atacccctgg aaaaatcgac ccaatcccgc atccgtatgg
    20341 tgaagatttg ccgtgcgcgg ataacaaacc ggtggcaccg aaaaagcagg aagccaaagc
    20401 cgtaaccgtg cagccaccgc gcccgaaacc gtgggaaaaa acctacgtgt tgctgccttc
    20461 tttcgagaaa gtgaagggcg ataaagtgct gtacgcccat gcttcgcgta ttctgcacca
    20521 cgaaaccaac ccaggctgcg tccgcgcatt gatgcaaaaa cacggtgacc gctatgtgtg
    20581 gatcaacccg cctgctatcc cgctttctac cgaagagatg gacagcgttt ttgcgctgcc
    20641 gtacaagcgc gtgccacatc cggcctatgg caatgcccgt attccggctt acgaaatgat
    20701 ccgtttttcg gtcaacatta tgcgcggctg ctttggcggc tgttctttct gttctattac
    20761 cgagcacgaa gggcgcatta ttcagagccg ttcggaagat tcgatcatta atgagatcga
    20821 agcgatccgc gacaccgttc caggttttac gggcgtgatt tccgatctcg gtgggccaac
    20881 tgccaacatg tatatgttgc gctgcaaatc gccacgcgct gaacaaactt gtcgccgttt
    20941 gtcgtgcgtt tatccggata tttgtccgca catggacact aaccacgaac cgacgatcaa
    21001 cctctatcgc cgtgcgcgtg atctgaaagg cattaaaaaa atcctcatcg cctcaggcgt
    21061 acgttatgac atcgccgtgg aagatccgcg ctatatcaaa gagttggcga cccatcacgt
    21121 cggcggttat ctgaagattg ctccggaaca taccgaagaa gggccgttgt cgaagatgat
    21181 gaagccgggc atgggcagct atgaccgctt taaagagctg ttcgatacct actcgaaaca
    21241 ggcaggtaaa gagcagtatc tgatcccgta tttcatctct gcgcaccccg gtacgcgtga
    21301 tgaagatatg gtgaatttgg cgctgtggct gaaaaagcat cgtttccgtc tcgaccaggt
    21361 acagaacttc tacccatcgc cgctggcgaa ctcaaccacc atgtattaca ccgggaaaaa
    21421 accgctggcg aagattggtt ataagagcga agacgtcttc gtaccgaagg gcgacaaaca
    21481 gcgtcgtttg cataaagcgt tgttgcgtta ccacgatccg gcaaactggc cgttaatccg
    21541 cccggcgctg gaagcgatgg gcaaaaagca tctgattggc agccgtcgcg attgcttagt
    21601 gcctgcgcca accattgaag agatgcgtga agctcgtcgc cagaaccgca atacccgtcc
    21661 ggcgttgact aaacatacgc cgatggcgac ccagcgtcag acgcctgcta cggcaaaaaa
    21721 agcgtcgtct acgcaatctc gtccggtgaa tgctggtgcg aagaaacgcc ctaaagcggc
    21781 ggttggacgt taaaaaaaat ccccggcaac tgacatgctg ccggggattt ttttatcatt
    21841 ctgcgtacat acgcaagcag cgcaggcaat caaaatagtc ctgagcatca agacgcgtat
    21901 tggctctgtt agcttcggat ttggcagcat agatttgtgg gcgtagtgct tttatatcct
    21961 gctcaagccg ggcgattttg gcatttaatg tttgtacatt gctcgccagt tgattgacct
    22021 tttgctcatt gacgcagcca ctgagcaagc aggttgccag aacaacagct cccacggtga
    22081 aaatcgtttt catatagtgt tcccttttat ttattgttcc ggctaattgt tatttaattc
    22141 agctcattgc tttaatctgg cggcggtttt tgcctcaatc tgcttaagta ggctgccgct
    22201 gttcgggcgg ataaaatcag cgatttccca cttgccttcg cgccgctgta aaaccagttc
    22261 ttgctcttcg ttgttgccat tggtcagcgt aaaacgaaca tgaatgcggg cggaatcggc
    22321 ggttttctcc aggactcgaa tttcatcaac gcgtgaaaat agcgactctt cgccaaaaca
    22381 gatcggattg tcgtaaataa attgccgttc tatttgcata tcttcatcgg gattattggc
    22441 cgtcttgaag gtggttttat agaaggcatt aaccgcctgg cggtaagagt cggaaaccac
    22501 ccacgtccac ggccagctat aatcagcaat atgttcaaag agttcggcaa caaaggcttc
    22561 cggctgttct ttttgcaacg aagctaaagc ggccagcgtt ttttcattct cgctattaac
    22621 tgcttgtaag acgctgccat gattgctgac aatatcgtca atgacccaac gaccattttc
    22681 cgccaccatt ttcagtgtct gcgtggtctt ttctttatca tctttaaaga tacgaaagcg
    22741 cacaacggca tcggcatgat cggcgtcagt ttgggttatc gcaacgcttt ctagcaccag
    22801 atcgccaaaa tcctgacaat cacaaaccgg atcataatcc agccagccaa tattgcccgg
    22861 cagcgtaaga ttgtcgttca gggtaagcgc ctgttgaata cgcgctgaag ttatcgcccg
    22921 ctctccggtt tcaccgaaat aaggtgtact ggcatctgat ttatagttct gataaatctg
    22981 ccggactgtt tgttctacgg tctgtgaagg aggctgcgcg tgtacggtaa aacttgccag
    23041 ggctgctaaa aacagaagta ttattttcat ctgtttttca cctctccgtt gcgtttatcc
    23101 cggatataga tgagttgttc ttttaagagc ttccggct
//
LOCUS       DAZPLM010000050        22941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPLM010000050 DAZPLM010000000
VERSION     DAZPLM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.050"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            19..714
                     /gene="araD"
                     /locus_tag="ACHVC7_001629"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="ACHVC7_001629"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR2115714.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="ACHVC7_001630"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="ACHVC7_001630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR2115715.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="ACHVC7_001631"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="ACHVC7_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR2115716.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="ACHVC7_001632"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="ACHVC7_001632"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR2115717.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHVC7_001633"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHVC7_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR2115718.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="ACHVC7_001634"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="ACHVC7_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR2115719.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="ACHVC7_001635"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="ACHVC7_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR2115720.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHVC7_001636"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHVC7_001636"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR2115721.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHVC7_001637"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHVC7_001637"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR2115722.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="ACHVC7_001638"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="ACHVC7_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR2115723.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="ACHVC7_001639"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="ACHVC7_001639"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR2115724.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHVC7_001640"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHVC7_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR2115725.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHVC7_001641"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHVC7_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR2115726.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHVC7_001642"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHVC7_001642"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR2115727.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="ACHVC7_001643"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="ACHVC7_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHVC7_001644"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHVC7_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR2115728.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="ACHVC7_001645"
     CDS             complement(19713..20999)
                     /locus_tag="ACHVC7_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2115729.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMNYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="ACHVC7_001646"
     CDS             complement(21050..21991)
                     /locus_tag="ACHVC7_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR2115730.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="ACHVC7_001647"
     CDS             complement(22006..22776)
                     /locus_tag="ACHVC7_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR2115731.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5528 a         6352 c         5975 g         5086 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc ttaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagttcatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa a
//
LOCUS       DAZPLM010000051        22840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPLM010000051 DAZPLM010000000
VERSION     DAZPLM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.051"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(83..562)
                     /gene="folK"
                     /locus_tag="ACHVC7_001648"
     CDS             complement(83..562)
                     /gene="folK"
                     /locus_tag="ACHVC7_001648"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR2115732.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(559..1956)
                     /gene="pcnB"
                     /locus_tag="ACHVC7_001649"
     CDS             complement(559..1956)
                     /gene="pcnB"
                     /locus_tag="ACHVC7_001649"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR2115733.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2016..2912)
                     /gene="gluQRS"
                     /locus_tag="ACHVC7_001650"
     CDS             complement(2016..2912)
                     /gene="gluQRS"
                     /locus_tag="ACHVC7_001650"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR2115734.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2979..3434)
                     /gene="dksA"
                     /locus_tag="ACHVC7_001651"
     CDS             complement(2979..3434)
                     /gene="dksA"
                     /locus_tag="ACHVC7_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR2115735.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3612..4316)
                     /gene="sfsA"
                     /locus_tag="ACHVC7_001652"
     CDS             complement(3612..4316)
                     /gene="sfsA"
                     /locus_tag="ACHVC7_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR2115736.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4331..4861)
                     /gene="thpR"
                     /locus_tag="ACHVC7_001653"
     CDS             complement(4331..4861)
                     /gene="thpR"
                     /locus_tag="ACHVC7_001653"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR2115737.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4935..7364
                     /gene="hrpB"
                     /locus_tag="ACHVC7_001654"
     CDS             4935..7364
                     /gene="hrpB"
                     /locus_tag="ACHVC7_001654"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR2115738.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7561..10095
                     /gene="mrcB"
                     /locus_tag="ACHVC7_001655"
     CDS             7561..10095
                     /gene="mrcB"
                     /locus_tag="ACHVC7_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR2115739.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10315..12504
                     /gene="fhuA"
                     /locus_tag="ACHVC7_001656"
     CDS             10315..12504
                     /gene="fhuA"
                     /locus_tag="ACHVC7_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR2115740.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12555..13352
                     /gene="fhuC"
                     /locus_tag="ACHVC7_001657"
     CDS             12555..13352
                     /gene="fhuC"
                     /locus_tag="ACHVC7_001657"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR2115741.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13352..14242
                     /gene="fhuD"
                     /locus_tag="ACHVC7_001658"
     CDS             13352..14242
                     /gene="fhuD"
                     /locus_tag="ACHVC7_001658"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR2115742.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14239..16221
                     /gene="fhuB"
                     /locus_tag="ACHVC7_001659"
     CDS             14239..16221
                     /gene="fhuB"
                     /locus_tag="ACHVC7_001659"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR2115743.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16379..17659)
                     /gene="hemL"
                     /locus_tag="ACHVC7_001660"
     CDS             complement(16379..17659)
                     /gene="hemL"
                     /locus_tag="ACHVC7_001660"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR2115744.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17825..17872
                     /gene="yadX"
                     /locus_tag="ACHVC7_001661"
     CDS             17825..17872
                     /gene="yadX"
                     /locus_tag="ACHVC7_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR2115745.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17884..19305
                     /gene="clcA"
                     /locus_tag="ACHVC7_001662"
     CDS             17884..19305
                     /gene="clcA"
                     /locus_tag="ACHVC7_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR2115746.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19329..19394
                     /gene="yadW"
                     /locus_tag="ACHVC7_001663"
     CDS             19329..19394
                     /gene="yadW"
                     /locus_tag="ACHVC7_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR2115747.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19387..19731
                     /gene="erpA"
                     /locus_tag="ACHVC7_001664"
     CDS             19387..19731
                     /gene="erpA"
                     /locus_tag="ACHVC7_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR2115748.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19778..20401)
                     /locus_tag="ACHVC7_001665"
     CDS             complement(19778..20401)
                     /locus_tag="ACHVC7_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR2115749.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20439..21239)
                     /gene="btuF"
                     /locus_tag="ACHVC7_001666"
     CDS             complement(20439..21239)
                     /gene="btuF"
                     /locus_tag="ACHVC7_001666"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR2115750.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21232..21930)
                     /gene="mtnN"
                     /locus_tag="ACHVC7_001667"
     CDS             complement(21232..21930)
                     /gene="mtnN"
                     /locus_tag="ACHVC7_001667"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR2115751.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22014..>22840
                     /gene="dgt"
                     /locus_tag="ACHVC7_001668"
     CDS             22014..>22840
                     /gene="dgt"
                     /locus_tag="ACHVC7_001668"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR2115752.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         5184 a         5801 c         6374 g         5481 t
ORIGIN      
        1 aaatgtttaa actaaaattt ttattaagga aagagaggat gtatgatttt cagaaaatat
       61 ttatattcgt aatataaata aattaccatt tgcttaattc gtcaaatgct cttgtatgta
      121 agacttcacg caacgtctcc ccatcaggaa acgccagctc cggtgcaatc tcaaacagcg
      181 gccacagcat aaatccacga ttcttcatat cgtagtgcgg aacggtcagg cgttcggtat
      241 ttatcacttc attaccaaac agcatgatat cgagatccag cgtgcgtggt ccccagcgtt
      301 cagctttacg aacacgaccc tgttgcagtt ctatgcgctg tgtatggttg agtaactctt
      361 ccggggcgag cgaagtttca agcgccacgg cggcgttgag gtaatcgggt tgatcttgcg
      421 gccccagcgg tggagtgcgg taaaacgaag aaacggcaag aatgtggctt tcagggatat
      481 cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga ttgctgccta
      541 tggcaatata cgccactgtc atgcggtacc ctcacgacgc ggtgcgcgtt tgcgtggacg
      601 acgagtacgg cgacgcggtg acggctcttc atccaactcg ttaagcatcc ctttttgatc
      661 tggtggcgcg gaaacctgga actcacccca ccatttcacc agacgctgca gttcagcgtt
      721 acgttcaact tcagctcgca aggccaacag gtcataagcc gcacggaact taggatgctc
      781 cagcagtttc catgcgcgtt taccctgacg tcgggacata cgcaactgca actgccagat
      841 atcgcgggtt aacgtcgtca ggcgtttcgg gattgccagt gaacggcagg cttcgtccag
      901 cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt cctgggcgat
      961 cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacaaga acgccgggtt
     1021 cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt caatgatccg
     1081 ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga acagcggctg
     1141 gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg cttgtagcag
     1201 tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg tagcgaggcg
     1261 agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg caaaacgtac
     1321 cgcgcgcagc atccgtaccg gatcttcacg gtagcgcgtt tccgggttac caatcagacg
     1381 gataacgccg tccttcagat ccttcatacc gccaacgtaa tcacggacgg taaaatccgc
     1441 tacgctgtaa tagaggctgt tgatagtgaa atcgcggcgc tgggcgtctt cttcgatgga
     1501 gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg tcgtgcggtc
     1561 gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct ccgggccaaa
     1621 cattacatga gccagacgga aacggcgacc caccaggcgg cagttacgga acagtttgcg
     1681 cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt tgccaagtaa
     1741 caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttgt tgagcctgta
     1801 cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct gctcacgcgg
     1861 gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct cgcggcttag
     1921 caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta gtgtcaaaca
     1981 tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt tgagaatgtt
     2041 gaatttacaa ttgccgactc gggcacggcg gtcagcgtcc agtttttgac tgctgactga
     2101 aggatttgtt cgacgctgaa atcctgccag tgtgcttctg cctgctgacc cagaaattga
     2161 agtgccgcga ttagtaccgg acgtggatcg cctttcggca acgcaggcgc atgattctgc
     2221 ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta atctggcact
     2281 ttccagccaa aaagctggta cagcgagatt tgccttactg ttggttcaat cagatcagcc
     2341 ccacgcacta tttctgtaac gccctggaaa tgatcatcaa ccacaacagc caggttgtag
     2401 gcgaacaacc catcacggcg atgaatgata aaatcttccc gtgccagttt ttcgtcggcg
     2461 tgaataatgc cgcgcagctg gtcagtaaat tgcgtgaccg gatgctgctg gcggatacgc
     2521 actgcggcat tgtctggtcc atgatgcaac acccggcaat gaccgtcgta aataccgcca
     2581 atgctttgaa tacgcgcacg cgtacaggtg cagtaataac ttagtccttg ttcatgtaac
     2641 caggcgagtg cttcacgata ggcgtcgtga cgttgcgatt gccagagaac atcgccgtcc
     2701 cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc gggaacttca
     2761 cgaggcgggt cgatatcttc tatgcgtacc agccagcgac cttgccgggc gcgagcctgc
     2821 aaatagctgc cgagcgcggc gatcagagag ccaaaatgaa gctcgccgga aggagagggg
     2881 gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata aggcgggagc
     2941 atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat ctgtttttcg
     3001 cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg cgcttccaga
     3061 cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc gtcttccact
     3121 tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg gttacgcagt
     3181 tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa gttggctgct
     3241 tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt acgccatgct
     3301 tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc gcccggcttc
     3361 tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt tttacggttt
     3421 tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg ttcgggggaa
     3481 aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta aacgtaacac
     3541 ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa attaaccagt
     3601 tacttacttt actacaatgt aaccgacagt gattttttaa gagccatgcc ttcagcagaa
     3661 atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga caatagttgc
     3721 gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga atgcagcacg
     3781 gcgaaaaaga taaccgcacg ctggccttca gccgctacgt tcatcaactc ccgaaggtgt
     3841 ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc cgctaacgta
     3901 accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat aaagtcaata
     3961 cggctgcgtt ctgcgccgta ttttacttcg cttttcagcg agctatagcc tgacagttct
     4021 gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag cgtattgacg
     4081 caaataaatg cgccgctctg gctttgagtt agttcccagg tgtgtgggta tttccgtttg
     4141 gtgttgtctg aagtcgaata ccagacggta tcgccacgcg ttgcacaacc ggtcatcgca
     4201 cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac atcggctaaa
     4261 aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagagaa ttccattgcg
     4321 acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt gtgcgtccac
     4381 gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc ataagaccag ttaaaacctg
     4441 gcggcgggat tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt ggatgaaacg
     4501 gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata ttcgccagct
     4561 ggattaagcc gcgtggcggc tgacgcatcc ctaaccacac cacacgcgaa cgcagccatt
     4621 gtccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg gctaaaagag
     4681 aaagcgcctt ctctttctcg gcgctcactt cgcctaaaaa tgccagagtc agatgcaaat
     4741 tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc cagtggataa
     4801 tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc ggttcagaca
     4861 tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag aatgttaacc
     4921 ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgaa ttacttaccg
     4981 ctctcgattg tgcgccgcag gtattattaa gtgcgccgac cggggccggg aaatcaacct
     5041 ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc ctgctggagc
     5101 cgcgtcgtct ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt aacgaaaagc
     5161 caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggcca aatacccgtc
     5221 tggaagtagt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg gagttgagcg
     5281 gtgttggatt ggtgatcctt gatgaatttc atgagcgcag cttgcaggcg gatttggcgt
     5341 tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg ctgattatgt
     5401 cggctacgct ggataacgac cgcttgcagc aaatgctgcc agaagcgccc gtcgtcatct
     5461 cagaagggcg ctcgtttccg gttgaacgcc attatttacc gctgcccgcg catcagcgtt
     5521 ttgacgaagc cgttgcggta gccaccgccg aaatgctgcg tcaggaaagc ggatcattac
     5581 tgttattttt acctggcgtc ggagaaattc agcgtgtgca ggaacaactg gcttcgcgca
     5641 tcggcagtga tgtgttgctc tgcccgctgt atggcgcgtt gtcgctgaac gatcagcgaa
     5701 aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc aatattgctg
     5761 aaaccagttt aaccattgaa ggtattcgtc tggtggtgga ttgtgcccag gagcgtgtgg
     5821 cgcgttttga gccgcgcacg gggcttacgc gactgattac tcaacgcgtt agccaggcat
     5881 ccatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatctgc ctgcatttaa
     5941 tcgccaaaga acaagcagaa cgcgctgccg cgcaaagtga accggagatc ttacaaagcg
     6001 atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg gcgcagatga
     6061 gctggctgga tcaaccgcca gtagtgaatc taatggccgc gaaacgtctg ttacaaatgc
     6121 tgggggcgct ggacggtgaa cggcttagtg cgcaagggca aaaaatggca gcgctgggta
     6181 acgatccgcg tttagcggca atgctggtta gcgcgaagaa cgacgacgaa gctgctaccg
     6241 cggcaaaaat tgccgccatt ctcgaagagc cgccactgat gggcaatagt gacctgggcg
     6301 tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg ttaaaacgct
     6361 taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt gccggggcgt
     6421 ttgccgatcg cattgctcat cgccgtgggc aagatggacg ctatcaactg gcaaacggca
     6481 tgggagcgat gctcgatgcc gacgacgcgc taagccgcca cgaatggttg atcgcaccgt
     6541 tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgcta ccggtcgata
     6601 ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc gtggaatggg
     6661 atgacgcgca aggtacgctg aaagcctggc gtcggctaca aatcggtcag ttgacggtga
     6721 aagtgcagcc gctggcgaaa ccgtcagaag acgagttgca tcaggcgatg cttaatggca
     6781 tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag ctacgcttgc
     6841 gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt gatgatgaaa
     6901 gtttattggc agcgctggaa acgtggctgc tgccacatat gacaggcgta cattcactac
     6961 gcggcctgaa atcactggac atttatcagg cactacgcgg attacttgat tggggaatgc
     7021 agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga agccggatcg
     7081 ccattcgtta tcatgaagat aacccgcccg cactggcggt gagaatgcag gagatgtttg
     7141 gtgaggccac taatccgact atcgcccagg ggggcgtgcc gctggtgctg gagttgcttt
     7201 cacccgccca aaggccacta caaatcacac gagatttgag cgccttctgg aaaggagcgt
     7261 accgtgaggt gcaaaaagag atgaaagggc gttatcccaa acacgtctgg ccggacgacc
     7321 cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg ttttctccct
     7381 ctccctgtgg gagagggccg gggtgagggc atcagcgtgc gcatttcccc ctcaccctaa
     7441 ccctctccct caagggagag gggaccgatc gagcacaaat tttgagagat atcttcttct
     7501 gtcttgtaac agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc
     7561 atggccggga atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa
     7621 caaaaggtaa gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat
     7681 gaggatgaag aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt
     7741 ggcaaacgcg gctggctatg gctgctgtta aaactggcta tcgtttttgc cgtgctgatc
     7801 gccatttacg gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg
     7861 caactgcccg cggcagttta tggccgaatg gtcaaccttg aaccagatat gaccatcagc
     7921 aagaacgaaa tggtgaagct gctggaggca acacagtatc gtcaggtgtc gaaaatgacc
     7981 cgtcctggcg aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat
     8041 ttcccggaca gcaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg
     8101 gcgacgatcg tcaatatgga gaacaaccgt cagttcggtt tcttccgtct tgatccgcgc
     8161 ctgatcacca tgatctcttc gccaaacggt gagcagcgtc tgtttgtgcc gcgcagtggt
     8221 ttcccggact tgctggtgga tactttgctg gcgacagaag accgtcattt ctacgagcat
     8281 gatgggatca gtctctactc catcggacgt gcggtgctgg caaacctgac cgccggacgc
     8341 acggtgcagg gagcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc
     8401 gagcgttctt actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt
     8461 tacagcaaag accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc
     8521 gacaacgaaa tccgcggctt cccgcttgcg agcttgtatt actttggtcg cccggtagaa
     8581 gagctcagcc tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac
     8641 aacccgtggc gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtttgcta
     8701 caacaacagc agattatcga tggtgaactg tatgaaatgc tcagcgcgcg tcctctgggc
     8761 gtacagccgc gcggtggggt aatctctcca cagccagcct ttatgcaact ggtgcgtcag
     8821 gagctgcagg cgaaattagg cgataaggta aaagatctct ccggcgtgaa gatcttcact
     8881 acctttgact cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca
     8941 ctgaagaaac agcgtaagtt gagcgatctt gaaactgcaa ttgtggtcgt cgaccgcttt
     9001 agtggtgaag ttcgtgcgat ggtgggaggt tctgagccgc agtttgcggg ctacaaccgt
     9061 gcgatgcagg cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct
     9121 ttaagccagc cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg
     9181 cgtcagccga atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc
     9241 ggcagagtga tgctggtgga tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg
     9301 gggatggcgc tggggctgcc tgcagttacg gagacctgga tcaaactggg cgtgccgaaa
     9361 gatcagctgc atccggttcc ggcgatgctg ctgggggcgt tgaacttaac gccaatcgaa
     9421 gtggcgcagg cattccagac catcgccagc ggcggtaacc gtgcaccgct ttctgcactg
     9481 cgttcggtaa tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc
     9541 gctgttccgg cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt
     9601 ggtacgggtc gtcaacttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg
     9661 actaccaaca ataacgtaga tacctggttt gcgggcattg acggcagcac ggtgaccatc
     9721 acctgggtag gccgtgataa caaccagccg accaaactgt atggtgccag cggggcaatg
     9781 tctatttatc agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca
     9841 gaagatattg cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg
     9901 cgtgtcttgc cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag
     9961 cagcagccgt caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag
    10021 caacctgctc agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg
    10081 tttggtagta attaacatct aagcgtgaaa taccggatgg cgaattgcca tccggtaaaa
    10141 taacatccca cctaaagtat taaccctttc ttttcatctg gttgtttatt aacccttcag
    10201 gaacgctcag attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt
    10261 tataataatc attctcgttt acgttatcat tcactttaca tcagagatat accaatggcg
    10321 cgttccaaaa ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca
    10381 gcggttagcg gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc
    10441 accgttaccg ctgcacctgc gccgcaagaa agcgcatggg ggcctgctgc aactattgcg
    10501 gcgaagcact ctgctactgc gactaaaacg gatacaccaa ttgaaaaaac gccgcagtct
    10561 atttcggttg tgacaaatga agagatgcag atgcgtcaat ttcagtctgt aaaagaagca
    10621 ttaggttata cacctggcgt tacggttagc agtcgtggtg cttctaatac atatgatttt
    10681 gttatcattc gtggtttctc atctgttggt ctgaatcaaa ataattacct tgatggacta
    10741 aaacttcagg gcaactttta taacgatgct gtgattgatc cttacatgct tgagagggtt
    10801 gaactgatgc gtgggccgac gtctgttctc tatggaaaaa gtaatcctgg tgggattatt
    10861 tctatggtga gtaagcgtcc gaccactgaa cccctgaaag aaattcagtt taaaatgggg
    10921 acggataatc tgtttcagac cggattcgat ttcagtgatg cactggatga taacggtgag
    10981 ttctcttacc gtttgacggg cctcgctcgc tcaacaaacg aacagcagaa aaactctgaa
    11041 tctcagcgtt ataccattgc tccatcattc tcatggcgtc cagacgacaa aaccaatttt
    11101 actttcctgt cctatttcca gaatgaacca gaaacgggtt attacggttg gttgccgaaa
    11161 gaggggaccg ttgagccatt gcctaatggt aagcgtctac cgactgactt caatgaaggt
    11221 gcatcgaata atacatactc ccgtaaccag aaaatggtgg gatatagttt tgaacatggt
    11281 tttaatgaca ccttcaccgt gcgtcagaat ctgcgtttca gtgaaatgaa aacctcacag
    11341 aaaagtgttt atggcacagg gattgctggt gatggtcata ccctaaaccg cgggacagtg
    11401 gtggataatg agcgtctaca aaactttagc gttgataccc aacttgaaag taagtttgct
    11461 acaggtgacg tcgagcatac tttgctgaca ggggtagact tcatgcggat gcgtaatgat
    11521 atcaatgcca gctttggatc cgcaccatcc atcgatcttt ataacaaata tcaccctgaa
    11581 tactttgcat ttggtagcgc agagccatac cagatgaatg aaagcaaaca aacaggtatt
    11641 tatgttcagg atcaggcgga atggaataaa tgggtattca ctctgggggg acgctacgat
    11701 tggtctaagc aagcgaccac tgttcgtgaa aactcttata cgccgactga aggttatatt
    11761 gagcgcaatg atcatcagtt cacctggcgc ggtggtgtaa attacttatt cgataatggt
    11821 atttcacctt actttagcta tagccagtcc tttgaaccga gtgctttcga tctgtggagc
    11881 aacccgcgca tttcctataa gccatcgaaa ggtgaacagt atgaagctgg cgtaaaatat
    11941 gttccgaatg atatgccggt cgttgttacg ggcgcagtct atcaattgac gaaaacaaat
    12001 aacctgacag cagacccaac aaacccatta gcgcaagtcc cagcaggtga gattcgcgct
    12061 cgtggtgtgg aacttgaagc aaaagcggcg ttaaatgcca atattaactt gacggcttct
    12121 tatacctaca ccgatgcgga atacaccaaa gacaccaatc tcaaaggtaa aactccagaa
    12181 caagtaccgg agcatatggc atctctctgg ggggattata ccttcaatga agggccgctt
    12241 tctggtttaa cattgggaac aggtggtcgt tttattggtt ccagctatgg tgatccggca
    12301 aacactttta aagtgggtag cgcagctgta atggatgctg ttgtaaaata tgatctggca
    12361 cgctttggta tggcgggatc cagccttgct gtgaacgtca acaatttgct cgatcgtgag
    12421 tatgttgcca gttgcttcca gacttatggc tgcttctggg gcgcagaacg tcaggtcgtt
    12481 gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggatttccg tgcccatttc
    12541 acaagttggc tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta
    12601 atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg
    12661 ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact ctgctcaaaa
    12721 tgcttggtcg tcatcagcct ccgtcagaag gggagattct tcttgatgcc cagccgctgg
    12781 aaagctggag cagcaaagcg tttgcccgca aagtggctta tttgccgcag cagcttcctc
    12841 cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg
    12901 cgctggggcg ctttggtgcg gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg
    12961 gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg
    13021 cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgactt
    13081 cggcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg
    13141 aacgtggcct gacggtcatt gccgtgttgc acgatatcaa tatggcggca cgctactgtg
    13201 attatctggt cgccctgcgc agtggtgaaa tgattgctca gggaacgcct gcggaaatta
    13261 tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattttg ccgcatccgg
    13321 cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg
    13381 tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc
    13441 ggcggctatt gaccccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct
    13501 ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc tgtgggtcag
    13561 cgaaccacca ttgccggact cagtgattga cgtcggtttg cgtacagaac ctaaccttga
    13621 actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc
    13681 agaaatgctg gcacgtattg cgccgggtcg cggatttaac ttcagtgacg gcaaacagcc
    13741 gttggcgatg gcgcgtaaat cgctgacgga aatggcagat ttacttaacc tgcaaagcgc
    13801 agcggaaacg catttagcgc aatacgaaga ctttatccgc agcatgaaac cccgctttgt
    13861 gaagcgtggc gcacgcccgt tactgctgac gacgcttatc gatccgcgcc atatgctggt
    13921 cttcggtcca aacagcttgt tccaggaaat tctcgatgag tacggcatcc caaatgcctg
    13981 gcaaggggaa accaacttct ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta
    14041 taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat
    14101 ggcaaccccg ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc agcgcgtacc
    14161 tgcagtctgg ttttatggtg cgacactctc ggcaatgcac tttgtgcgcg ttctggataa
    14221 cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt attgctggcg
    14281 ctgttagtga ttgtcgctac ggcgctcacc tggatgaact tctcgcaggc gctgccgcgt
    14341 agccagtggg cgcaggctgc ctggtcgcca gacatcgatg tcatcgagca gatgattttt
    14401 cactacagct tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg
    14461 gtgggcgtgc tgtttcagca agtgctgcgt aacccgctgg cggagccgac gacgcttggc
    14521 gttgctacag gcgcgcaact gggaattacc gtcaccacac tttgggcgat cccaggtgct
    14581 atggcgagcc agtttgccgc actggtgggc gcttgtgtcg ttggcttaat cgtctttggc
    14641 gtcgcgtggg ggaaacggct ttcgccggtt acgcttattc tcgcggggct ggtggtgagc
    14701 ctttattgcg gggcaattaa tcagctactg gttatcttcc atcacgacca gctgcaaagc
    14761 atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggcgt tgagcgttta
    14821 tggccgcagt tgctgggcgg cgtgatgttg acgttattgc tacttcgccc gttaaccctg
    14881 atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct cgcgcgtctg
    14941 gcggcgctgt cgctggcgat agtcatcagt gcgctgctgg tgaatgcggt ggggattatc
    15001 ggctttatcg gtttgttcgc gccgctgctg gcgaaaatgc tgggggcgcg gcgtttgttg
    15061 ccacggttga tgctggcgtc gctgattggg gcgctgatcc tctggctttc cgatcaaatc
    15121 atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc
    15181 ggtgcgccgc tgctactgtg gctgttgccg cgtttacgca gcattagcgc gccggatatg
    15241 aaggtcaacg atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc
    15301 ggcgtgctgc tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc
    15361 tggacgtggg cgagcggggc gttgctcgag gatttgatgc cctggcgctg gccgcgaatt
    15421 atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcggctg
    15481 accggaaacc cgatggcaag cccggaagtg ctggggatta gctctggcgc ggcgtttggc
    15541 gtggtgttaa tgctgtttct ggtgccgggt aatgcctttg gctggctgtt acccgcgggc
    15601 agtatcgggg cggcggtgac gctgttgatc attatgatcg ccgccggccg cggtggattt
    15661 tccccacacc gtatgttact ggcggggatg gcgttaagta ccgcattcac catgcttttg
    15721 atgatgttgc aggcgagcgg tgacccgcga atggcgcaag tgctgacctg gatttccggt
    15781 tcgacctaca acgcgaccga tgcgcaggtc tggcgtaccg gaattgtgat ggtgattttg
    15841 ctggcgatta ccccgctgta ccgccgctgg ctgaccattt taccgctggg tggtgatacc
    15901 gcacgagccg taggaatggc gctgacgccg acgcgaattg cgctgctgct gttagcggct
    15961 tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca
    16021 ccgcatattg cgcggatgat gggctttcga cggacgatgc cacacatcgt aatttcggcg
    16081 ctggtgggtg gtttactgct ggtattcgct gactggtgtg ggcggatggt gctgtttcca
    16141 ttccagatcc cggcggggct gctgtcgacc tttatcggcg cgccatattt tatctatttg
    16201 ctgagaaagc agagccgtta atttttccat ataacggccc tgtacgcctg gacggataaa
    16261 gcgtttacgc gacaacggat gcccgatgcg acgctggcgc gtcttatcgg gcctacaaac
    16321 ggccccgaat cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaacc atgctggatc
    16381 acaacttcgc aaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt
    16441 gcgccacgga cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcgtcca
    16501 gcatcatatg gaagaaacgc ttaaagcgtt ccacgtcaca ggccatcaca tcctgatagc
    16561 acgtcacgga ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggttaacga
    16621 ccagcggaat tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca
    16681 gctcatccaa cgtttcgtga acgcccggtt gcgcgacttc attcagacag gcgaaaccgg
    16741 ctgccatcgc aattgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg
    16801 ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga
    16861 tgattttgcc caggcaggtg agatccggtt ccacaccgta ataatcctgt gcgccagcca
    16921 gtgccacgcg gaagccggtc attacttcat cgatgatcag caatgcgcca aattcgtcgc
    16981 acagcgcacg cagacctggc aggaagtctg gcagcggtgg aacacagttc atattgcctg
    17041 ccaccggctc gatgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta
    17101 cagaagccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc
    17161 ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt
    17221 cagcgtgacc gtggtaacac ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac
    17281 gggccagacg gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat
    17341 ccatggtcgg gaccaattcg gtcaccagtt gcgccatttt cacttccatt tcggttggtg
    17401 caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatt gccggatggt
    17461 tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa
    17521 catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag
    17581 taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa
    17641 gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt
    17701 ctatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa
    17761 tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat
    17821 tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat
    17881 tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga
    17941 caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg
    18001 gcagtcgtcg gcacgcttgt tgggctggca gcggttgctt ttgacaaagg tgtcgcctgg
    18061 ttgcagaacc aacgtatggg ggcgctggta catactgctg ataattatcc gcttctgtta
    18121 accgtcgctt ttctctgttc ggcggtgctg gcgatgtttg gctacttttt ggtgcgcaaa
    18181 tacgcgccgg aagcaggtgg ttcggggatc ccggaaattg aaggggcgct ggaagatcaa
    18241 cgtcccgttc gctggtggcg tgtattgccg gtgaagttct ttggcgggct ggggacactc
    18301 ggcggaggca tggtgttggg gcgcgaaggg ccaaccgtgc agatcggcgg taacattggc
    18361 cgtatggtgc ttgatatttt ccgcctgaaa ggtgacgaag ctcgccatac gctgctggca
    18421 accggtgctg ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt
    18481 attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgataaa agcggtattt
    18541 attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt
    18601 gacgtcggta aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt
    18661 attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg
    18721 ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggcgg tgcgattggc
    18781 ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg
    18841 attcctatcg ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct
    18901 cgggtcatta ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg
    18961 atgctggcgc tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt
    19021 ccgcaatatc accttgaggc ggggacgttt gctattgccg gaatgggggc attactggcg
    19081 gcatctattc gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac
    19141 cagctcattt tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc
    19201 ggcgggaaac cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag
    19261 caactggcgc gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt
    19321 attagataat ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag
    19381 caaaatatga gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa
    19441 gttaaaagcc tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcaca
    19501 ggtggcggtt gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc
    19561 gatatgacca tcgaaaaaca gggcgttggc ttggtggttg atccgatgag cctgcaatat
    19621 ctggtcggcg gttccgttga ctataccgaa ggactggaag gttctcgttt catcgtgacc
    19681 aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt
    19741 catgtgccgg ataaactatc cggcgcatct tctctcttca acgcccattc tcatccagcg
    19801 caaacgtcgg tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga
    19861 ccatgcccat catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt
    19921 ggacaatacc gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga
    19981 tctcgcgggc cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgaccatac
    20041 aaaccgcgat taacggaccg acttccgcat taaaggcttt attcacgcca atgccgacaa
    20101 acaccgccag accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct
    20161 ggcgcaccag cacgatggtc agcatgctgg ttaccattgc aacgaccaga tcggtgggat
    20221 ctttcaccca aaataccggg ccgtgatcca gcgccatgtc gcgaattgtc ccgccgccta
    20281 cagcggtaac cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta
    20341 acaaaacgcc ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca
    20401 ttgttcaatc cccactgagc acctaaaaag gtcaggcgct aatctacctg tgaaagcgca
    20461 ttacagagct gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc
    20521 gtgagaggaa taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt
    20581 tggtccggtc cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg
    20641 ctaacttgcg gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg
    20701 agtacctggt tttgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc
    20761 agaaaaacac gttttttagg tttatcagca tattgcgctt tcaattgcgc gtactgatcc
    20821 agcagggatt gcgcggcttg ttcggcctta tccggttgcg gactccaggg ggccaattga
    20881 cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc
    20941 agcgaagcca gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga
    21001 tcgggtttca gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca
    21061 atcttttgcg cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg
    21121 gcggcaaagg caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg
    21181 agccacagtg gcgcaagaaa agacagggcg accagcgccc tgaacagtga cttagccatg
    21241 tgccagtttc tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag
    21301 gaactcatcg aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac
    21361 aacaaacggg acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac
    21421 agcaatggcc tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa
    21481 agcgtcgccg ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc
    21541 ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg
    21601 accgtattca taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac
    21661 aacgatatcg cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat
    21721 cacatctggc ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc
    21781 gatgcccgat ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca
    21841 accgccgaga ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc
    21901 ttccattgca ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaaa
    21961 gcatagttta ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac
    22021 agattgattt ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta
    22081 aaaccgaaca tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc
    22141 cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg
    22201 tgcgcacgcg tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag
    22261 aaattttaag ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga
    22321 ccggcccctt tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc
    22381 cgtttggtca ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg
    22441 aagatgccga aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac
    22501 gggacgggga agaacagctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt
    22561 ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt
    22621 gggcacaggt tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc
    22681 ctgagacaca tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata
    22741 ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga
    22801 ttatggaagc tgccgacgac atctcctatt gtgtggcaga
//
LOCUS       DAZPLM010000052        22447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPLM010000052 DAZPLM010000000
VERSION     DAZPLM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.052"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHVC7_001669"
     CDS             complement(<1..223)
                     /locus_tag="ACHVC7_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR2115753.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHVC7_001670"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHVC7_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR2115754.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHVC7_001671"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHVC7_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR2115755.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHVC7_001672"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHVC7_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHVC7_001673"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHVC7_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR2115756.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHVC7_001674"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHVC7_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR2115757.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHVC7_001675"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHVC7_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR2115758.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHVC7_001676"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHVC7_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR2115759.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHVC7_001677"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHVC7_001677"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR2115760.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHVC7_001678"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHVC7_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR2115761.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHVC7_001679"
     CDS             7306..8433
                     /locus_tag="ACHVC7_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR2115762.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHVC7_001680"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHVC7_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR2115763.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHVC7_001681"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHVC7_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR2115764.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHVC7_001682"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHVC7_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR2115765.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHVC7_001683"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHVC7_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR2115766.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHVC7_001684"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHVC7_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR2115767.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHVC7_001685"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHVC7_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR2115768.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHVC7_001686"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHVC7_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR2115769.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHVC7_001687"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHVC7_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR2115770.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHVC7_001688"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHVC7_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR2115771.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHVC7_001689"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHVC7_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR2115772.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHVC7_001690"
     CDS             14427..15242
                     /locus_tag="ACHVC7_001690"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR2115773.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHVC7_001691"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHVC7_001691"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR2115774.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHVC7_001692"
     CDS             complement(17104..17286)
                     /locus_tag="ACHVC7_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR2115775.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHVC7_001693"
     CDS             complement(17365..18282)
                     /locus_tag="ACHVC7_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR2115776.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHVC7_001694"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHVC7_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHVC7_001695"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHVC7_001695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR2115777.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHVC7_001696"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHVC7_001696"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR2115778.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHVC7_001697"
     CDS             complement(21301..21957)
                     /locus_tag="ACHVC7_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR2115779.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHVC7_001698"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHVC7_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR2115780.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5508 a         5903 c         5688 g         5348 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    22441 aacagca
//
LOCUS       DAZPLM010000053        22407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPLM010000053 DAZPLM010000000
VERSION     DAZPLM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.053"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHVC7_001699"
     CDS             <1..411
                     /locus_tag="ACHVC7_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR2115781.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(459..1331)
                     /locus_tag="ACHVC7_001700"
     CDS             complement(459..1331)
                     /locus_tag="ACHVC7_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR2115782.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(1343..2437)
                     /locus_tag="ACHVC7_001701"
     CDS             complement(1343..2437)
                     /locus_tag="ACHVC7_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR2115783.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2470..3468)
                     /locus_tag="ACHVC7_001702"
     CDS             complement(2470..3468)
                     /locus_tag="ACHVC7_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2115784.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3493..5004)
                     /locus_tag="ACHVC7_001703"
     CDS             complement(3493..5004)
                     /locus_tag="ACHVC7_001703"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR2115785.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(5027..6010)
                     /locus_tag="ACHVC7_001704"
     CDS             complement(5027..6010)
                     /locus_tag="ACHVC7_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR2115786.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(6107..9384)
                     /locus_tag="ACHVC7_001705"
                     /pseudo
     CDS             complement(6107..9384)
                     /locus_tag="ACHVC7_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            9502..10695
                     /locus_tag="ACHVC7_001706"
                     /pseudo
     CDS             9502..10695
                     /locus_tag="ACHVC7_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHVC7_001707"
     CDS             complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHVC7_001707"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR2115787.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHVC7_001708"
     CDS             12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHVC7_001708"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR2115788.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHVC7_001709"
     CDS             complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHVC7_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR2115789.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHVC7_001710"
     CDS             complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHVC7_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR2115790.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHVC7_001711"
     CDS             complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHVC7_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR2115791.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHVC7_001712"
     CDS             complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHVC7_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR2115792.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHVC7_001713"
                     /gene_synonym="purI"
     CDS             complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHVC7_001713"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR2115793.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         5253 a         6237 c         5689 g         5228 t
ORIGIN      
        1 cgttattttg attttggagc tgcccgcccc gttttgttat taattgcccg tatcgccgtg
       61 gtcttaattt tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa
      121 tatatggcct cgttgggcgc gccaatgccg atgctggcag cgattattgc ggtagttatg
      181 gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca cccgtccgct ggcggtgctg
      241 tttattttct acacgctggg tacggcggtg attggtcacc attactggga tatgaccggc
      301 gatgcggttg ggccaaatat gattaatttc tggaagaatg tcagtatcgc tggcgcgttc
      361 ttgctattgg caattaccgg gccgggggca atttctctcg atcggcgtta ggaaagatgc
      421 cggatgcggc gtgaacgcct tatccggcat ttaataaatt acagccactc tacccgcaac
      481 gacatctctg aagttgttga ttcccctggt gctagcgcaa tgagatcacc cccttccggg
      541 cgatgatgat catccggcgc atggctcatc ggttccagac agaagaaatc aaacgcatat
      601 cctttatcaa acgcagggtc ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt
      661 tccatgatga tggcataacc ttcctgcggc tgctcgatgc gggcctgacc attccatccg
      721 gcaaaaccat tgttcaccca ctggcgcggc aacggcgcga gctggttaaa atccagttcc
      781 tgcggtagct gctcgcaaaa ctcacccgcc agccactgct cccgttccag ccagtaaccg
      841 ctcgcctgcg cctgaatccg cgtttgtggt gacaacggaa aataaggatg ccagccggta
      901 ccaaatggca gcgtctctgc cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc
      961 gccgttaaat gaaacgcctg acttacccga tagtgataga caccgctgcg atgttcatac
     1021 accagacaca aactatcatc gctgtgcgag acacattgcc actcgcccag ccagccatcg
     1081 ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca gttgatactc acgcccctgc
     1141 cagacaaaac ggttgccgct cacccggttt gcaaatggca ccagcggaaa acatgaggca
     1201 tcggttgcca caccgctttt tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag
     1261 ccttcgatta cgccgccctg gtcagaaacg tccagcttta gcgacccgtg ggataaggta
     1321 tagatggtca tccgctaact ccttaatccg ggaagttaat cacaactttc ccgcatttgc
     1381 cgctcgccat cagcgcatag gcatcacctg cctgttccag cgagaagcga tgggtaatgg
     1441 cgttacgcgg ccacagcttc cagtcagtca aatcgtgggc gcatttttcc atatggaaca
     1501 gactggtcac ccaggagccg ataatccgcc gttgatggtg catcagatcg gcgctgacct
     1561 cgaattccac ttttccggtt tcaccaatgt aaaccacccg tccccagtca gcggtggatt
     1621 gcagtgccag caagcgacct gcggcattac cagaacaatc gagcgcaaca tccgcgccac
     1681 cgtgggtgag ttcggcgata atctgcggca gaccttcggt ggttgctaaa tagccgtgat
     1741 ccatcacccc taactgtttt gccatcgcca gacgttccgg cagcatatca acgccgatga
     1801 tccgttttgc accgcgacct ttcgccagca tcatcgccat catgccgacc ggccccagac
     1861 cgaccaccag cacgttatcg ctgccggaaa cttcgccgcg caaaatccct tcataggccg
     1921 tgccaacgcc gcaactgata aacgcaccat cttcgtagct cagcgcatcc ggcaggagga
     1981 tcagatcttt ttcttccgcc agcagatatt cggcatgacc gccgtcacgc tgccagccgt
     2041 aagccgcttt tcctttgcca gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa
     2101 aaccacagcc agaaatgtga tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc
     2161 cttgccccat cgccacaatc tgcccgcacg gttcatgacc gttgataaat ccctggtata
     2221 acggtttatc gggtgccgcc gccgtggcac agtgttgatg atagatatag tggacatcgc
     2281 ttccgcaaat cccggaggat ttcattttga tcagtacctg gttaatcccc ggcgtcggca
     2341 ccgcaacttc ccgcagatcg acggttgaat ttcctggtaa ataagctgcc agcatcgttt
     2401 tcataaatcc ctcattaaca atacgattaa ttttcatccc tgccgcaccc gcgccagggg
     2461 cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc agcaagatat tcgccagcac
     2521 cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa aaggagttaa taccgagcag
     2581 caccagaccg ttaccgatta gcgtaatcac cagcacacca agcaatgtac cgaacaggga
     2641 accgcgaccg ccggaaagtg ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc
     2701 cagaccgttt gcggcacctg cgttaccgga accgaggcgt gccgccagca aaatgccggt
     2761 caccgccgct aataatcccg aaagggtaaa gataagaatg cgcacccgac gaacgttaat
     2821 accgcacaac tgcgccgccg tggcattacc gccaacggca aaaaccgagc gcccgaaggc
     2881 ggtttttcgg ctaatgaaca caaacagcgc aaacaacacc atcatgatca gcgcggatac
     2941 tggcacaccg agaaattgtc cgcccagcca gtccagcacc tcgttttcgt caatcggcac
     3001 tggcagtgcg ttggtcataa acagccccat tccgcgcagg gcgctccaca gccccagtgt
     3061 ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag
     3121 cgcgcctaac agcaacacca gcagacaagc aatcgccaac ggaacttcaa attgcagcaa
     3181 aaatgccagg cacaccgaga caaaagccac catcggccca acgctgacat caatttcacc
     3241 ggagataata atcagcgtca tcgcccaggc ggcaatccca atggtggcgg catcgcgcag
     3301 cacgttcatc tggttattca atgagataaa gccaggcgcg ttcagggaga agaccagata
     3361 aagaatggca atcaccacca gcaaaccgat ctcattaata tggcgactga caaattgttt
     3421 gagcgagacg ctcttgccct gcggcaatgg taatgacgaa gcagacatct tcaggttcct
     3481 cggtaaaatt catcagtgca cagacagaat ggcggacatc agctcatcca cattgaccgg
     3541 cgagtgaaac tcctgcgaga acgtgccgtg ctgtaataac aggatgcggt cacacaccag
     3601 cggcaactcc tccacttcac tggagataaa caccacgctt tttccttcgg cagccagctc
     3661 acggacaata cggtaaatct gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc
     3721 gagcaacaaa atctggctgg cggcatagac ccaacgaccg atcaccactt tttgctgatt
     3781 gccaccagaa agcgtgccga tgggcgtttc gctactagcg gccttgaccg tcatccgctg
     3841 catcacctct tcggtcaggc ggcggatggt ggaccattgc agcactccgt tggcgctgat
     3901 tttttgccga ttggtcagca ctgtattttc gtcaacgccc aaccagggaa tgatccccgc
     3961 ttctttgcgg ttttctggcg tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg
     4021 gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt tcatacgtct ccagcccaac
     4081 aatcgccttc agcaattcac tgcgccctgc ccccagcagg ccagcaatgc caagcacttc
     4141 gccgcgacgt aacgtaaagc tgatatcctc cagcttgggc ttatggcgta acgcacggac
     4201 ttccagtacg gcctgatcca caatttcctg aggggcaacc ggcgcaatat caacgtgatc
     4261 acgccctagc attagcgaca caatatgatg cgtggaggtg ttttcgagca tcacatcgcc
     4321 cgccacctga ccatcgcgca taacggtggc acaggaggca atgcggcgga tttcttccat
     4381 ccggtggctg acataaatca ccgccacgcc cagtgccgac atctttttca ccgcactgat
     4441 caccagttca acttctgcac tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg
     4501 cggctcgccc ttcatcaccc gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt
     4561 tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc
     4621 ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga ggccattgac caaggcagag
     4681 gttttctgcc accgtcagcc cttccaccag acttaattcc tgataaaccg cacgaacccc
     4741 cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat
     4801 ccagatatca ccgctatccg ggcgttcgct gccggtaagc attcgaatga gagtcgattt
     4861 gcccgcgccg tttttaccta acagcgcacg aacttcgcct ttattgagcg tgaagttaac
     4921 gttatccagc gcaacgacgc cggggtaacg cttatttcct gccaccactt ttgctaccgg
     4981 gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag ggcagaccat
     5041 caacgtgcgt tgccagccac tgtttaccat cttccgtttt ggtatagaga tcgattggca
     5101 cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa
     5161 cagcattgcc cattttctta ccggaaatat ccactaccgc tttcagcacc tgattgtttt
     5221 ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa aacagcaatt tttccggcct
     5281 gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc accgcccgac tcccccataa
     5341 tggcgttgag atccggcgtg gagataatca gtttttcacc aacggaaatc gctttattta
     5401 aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggat tttaatactt
     5461 cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa
     5521 ttttcggctg gtcaatttta ttggcaataa aataatcggc ggcagcgtta cccagttttt
     5581 taccaaattc cagtggatcg ccgaccagat acgccgagac atatttatcg acaccctttt
     5641 gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg cgacgaacgg
     5701 tacggctact gccattttca gacactgccg ataaaataat ggcatcgaca ttacgcgcca
     5761 cgagggtatc aacaaaggta ctttctttcg aaatatcacc ctgggcgtta gtttcaatta
     5821 actgtacctg tactgaagaa tcttttgccg catcctgaac gccctgacgc actccagcgt
     5881 aatatcccag ggtatcaagg tatattgcac caatggtcat ttctttatcc gcagccctgg
     5941 caaatagtgc gctacctaat agcgtagcca ttaataatag attacgggtt gttctcattt
     6001 ttttaggcat agtgcctcct gtagagtttt tattaacaac ggcttattct aattattttg
     6061 tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag tgaacgtagt
     6121 taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggcc ggattaatcg
     6181 ccgtcaattc agcgcgagca gattcatatt gcgcaacctc cccgagtccc gcggacgcca
     6241 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgaca
     6301 acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat actggttttc gccatctctt
     6361 gcgcccactg tttcatttca ctaaacagtt gctgtgcgat ttgttgttcg cccagcagtc
     6421 gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa taactgtgga
     6481 tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat
     6541 cgccctgcgc gttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
     6601 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgcagg
     6661 cctgctgcgg ctgtctggca tcaagatgct gccaggcgcg taataactgg ttgaggataa
     6721 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa
     6781 tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa attgagcagt tctgcggtca
     6841 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
     6901 gtgtggctcc acttaactta tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg
     6961 gcgcaagctg ataagcatta tcaagataac gcgcggccag ttcataatcg tgttgcttat
     7021 tccacgcatg gatcgccaac ccgcgccagc catcggcaaa ctccggcgac atctctacgc
     7081 aacgttgcca taaagtaatg gctttgttgt agctgcgctt gttgtagtag aagcaggcca
     7141 gtaaatgacg ggcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
     7201 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccgc
     7261 gttcggcttt cggcagcaag ctggcttgta gataaagcga taacgtgcgc tgacagtcca
     7321 gtgcgttcag catctctgtc gccagagtgg gcattcccca gttaatcagt tgcccggcgg
     7381 tcatcagggc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agggctgatt
     7441 cgctacgacc atcgaaccag ttcagccacc acagaatggc gttcagcgga taatcacgca
     7501 gcagtttcgc gcgctgcaaa cgcgcgttgt cctgacgacc acttaacacc agcagcagat
     7561 tatgcaggca aagcacttcc tgattggttg ggcaggcgcg aagactttgt tggcaaaaat
     7621 ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg tgccaggcca taatagccac
     7681 cggctttgct gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
     7741 gtccctgacg ttcgtaagca ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt
     7801 tgttcagcgc atgtgcgcgt ttcagcgcct gactggcata cgccaccgct tgcgggaaat
     7861 ctgcgcggtt atattccagc atcgccagcg ccaggttaca gcgataatcc agcgggtcca
     7921 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
     7981 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gccagtggcg
     8041 ctttggcgac gtccggtaac ggcaacgctt gcggctgatg ttcctgataa ctcagtacaa
     8101 tattgccgtc ggcatcggaa agctcaatgg tcagcctatc aggattgata ccgtgcaaca
     8161 cgccctggat ggcggtcgca ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt
     8221 tgccgatttc gtggatcgcc aggcggtatc cgttcaacgg agagatggca tacagccccc
     8281 actcaatccc ctgctcacta cgctggagtt ttatcaccgc atcgcgggag gcattttgca
     8341 ccatgcccag agaatgataa ggcaggaaat actgctcaaa acgcttctct tcgtaagcat
     8401 caagccaggt aaaatcgggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
     8461 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
     8521 tccactgttt tttacctagc gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt
     8581 catcgtgaca ccacgcgccg acaaaatcgt actgcgattt ttcggccata tatgaggttg
     8641 gaacgggcac atttttatag cgagaaatgt ccactccagc ggagtagtcc actttgtagt
     8701 aagtgccggt agcgatgggg aaagcggaga cggcccgttt gccgtgatca aacaccgcag
     8761 tcaaatccgg tgggaaaacg ctctgatgcc cttcaccccc tttcactgcc gggttggccc
     8821 accacaagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagcg
     8881 ccgcccaggg cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc
     8941 gcctacccac accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat
     9001 aaaggtggtc gggcgatgat gttgcggcca gttaaactca atcccaccgg aaatccacgg
     9061 ccccagcagc cccaccagcg caggtttaat gacttcattg tgataaacaa aatcgcgctg
     9121 tttcacttta tcccacgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt
     9181 gatgtagtcg ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag
     9241 cgtatcggtc acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc
     9301 caggaacatg ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc
     9361 ttgccacact tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa
     9421 agatttgtgg cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta
     9481 actaaacaag gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat
     9541 aacaaatgcg tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg
     9601 gcccggctgg cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc
     9661 gaaaaacggg tggtgaatat cgacgatgaa agccagacgc gcgggcatag tagcggtaca
     9721 tggctgattg cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt
     9781 gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt
     9841 gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt
     9901 aaattgtggc cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatccg
     9961 gtgaccggcg tgtcgcaaac catgccgcaa gcgccgtgga caacgccggt tgaggtgaag
    10021 tatctgctgg aagagaagct cggcattcgg gtgatggtcg ataatgactg cgtgatgctg
    10081 gcgctggcgg agaaatggca aaataattcg cagaaacggg atttctgcgt gatcaacgtt
    10141 gattacggca ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat
    10201 ggtagcggac agattggtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga
    10261 cgttacggct gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta
    10321 tggctaaaat cacaaccggt taatactcag cttgatccgg aaaaactgac tacagcgcag
    10381 ttaatcgctg cctggtagag tggagaaccg tggatcacca gttgggttga tcgcagtgcc
    10441 aatgccattg gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg
    10501 ttgtacggtc gcagttgtgc ctttggtgag cactggctta ataccattat tcgccagacg
    10561 ggatttaacc cgttcgaccg cgacgaaggg ccgagcgtga aagcaacgca aattggcttt
    10621 gggcaattaa gccgcgcaca acaggtgttg gggattggct atttgtatgt tgaggcgcag
    10681 ttacgacaga tttgatggcg caataacgta gaaaggcttc ccgaaggaag ccttgatgat
    10741 cacataccta agattgcctg atgcgctacg cttatcaggc ctacacggat attgcaatat
    10801 attgaatttg caaagttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    10861 acgagcacat tgacagcaaa tcaccgtttc gcttatgcgt aaaccgggaa acgtgcgcag
    10921 atgtcgagaa ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc
    10981 acgtcacaca tccagccagc cagttctttc gcttctgctt ctttgaagcc gcgacgcgta
    11041 atcgccggag tacccacgcg aataccggag gtcacaaacg ggctcttcgg atcgttcggt
    11101 acgctgtttt tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg
    11161 gtcaggtttt tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc
    11221 actttgtagc cgcgctcaag gaacacttct accatcgctt tagcgttttt cgcgacctgc
    11281 tgctggtaag ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg
    11341 attacgtgca tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg
    11401 tacagctctt cgctaccacc tttcgccagg atcaggccgc cgcgcggacc cgccagggtt
    11461 ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca
    11521 gcaaccaggc ccgcaacgtg cgccatatca acgaacaggt aagcaccgat gctgtcagcg
    11581 atttcacgca ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc
    11641 attttcggct tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat atgaccggta
    11701 gcatcgatac cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg
    11761 tgagtcaggt gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc
    11821 agcgcggtgt agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag
    11881 tcagcgccga acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac
    11941 tcgcaaccgc cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga
    12001 gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg
    12061 tgctcttcct gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg
    12121 gcaatgttca tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt
    12181 tggcctttat aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga
    12241 taacaggtct tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg
    12301 gagaataaag agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt
    12361 cacttcatca cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt
    12421 ttataagatg catttgagat acatcaatta agatgcaaaa aaaggaagac catatgcttg
    12481 acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa
    12541 agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt
    12601 ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg
    12661 cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc
    12721 agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt
    12781 tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag
    12841 cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg
    12901 ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc
    12961 gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat
    13021 accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa
    13081 ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt gcggtgaaac
    13141 gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg
    13201 tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga
    13261 cgttaatctc tgccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa
    13321 aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc gatgttcacg
    13381 cctttgccga tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct
    13441 ggcatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga
    13501 tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg
    13561 gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg
    13621 aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat
    13681 ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac
    13741 ggatgcgaat gacccgtgcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg
    13801 tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa
    13861 tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg
    13921 tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga
    13981 cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct
    14041 ttttcatgct attccttgaa aaggtcgcct gtcttttgat ctgctaaacg taacacataa
    14101 cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa
    14161 attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt
    14221 gcagcagctt acgcaaatag ttgagttcaa actgattacg tgcctcaaca aaggttggca
    14281 gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg
    14341 aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc
    14401 tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc
    14461 gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg
    14521 ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt
    14581 ccccgcgcgc catcgctttt ggcagatcac ggtgagtggc agaaataatc cgcacattga
    14641 tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca
    14701 gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt
    14761 ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat
    14821 gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa
    14881 atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg
    14941 tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt
    15001 ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg
    15061 tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga
    15121 caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag
    15181 aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa
    15241 atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt
    15301 cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt
    15361 cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata
    15421 aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca
    15481 ctctcgtgcg gcgtatccgg cgagaaattt ccagtcggtt tgcgggtcga gagctggcgt
    15541 tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg
    15601 tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa
    15661 cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac
    15721 agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg
    15781 cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt
    15841 tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga
    15901 tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc
    15961 agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt
    16021 ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat
    16081 gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt
    16141 cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga
    16201 catggacagc gcggagaaac cggagcgtac atatcgtacg tgaagatttc gagcactgcc
    16261 cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt
    16321 ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat
    16381 acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc
    16441 ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc
    16501 gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat
    16561 gtgaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt
    16621 aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga
    16681 gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg
    16741 tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac
    16801 ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg ctatcaagaa
    16861 tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctggtca gccagtaatt
    16921 cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa
    16981 attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc
    17041 caaccgagcg taactcgctc ggtccactga acgaaacgct attgcccaga gaacgccctt
    17101 cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga
    17161 aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt
    17221 gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg
    17281 tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag
    17341 catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg
    17401 cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac
    17461 gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac
    17521 ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa
    17581 taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg
    17641 cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt
    17701 gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga
    17761 atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt
    17821 tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc
    17881 tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac
    17941 gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact
    18001 gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat
    18061 ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag
    18121 ataaaattat tagattacaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt
    18181 aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga
    18241 tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc
    18301 caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc
    18361 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    18421 gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc
    18481 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    18541 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    18601 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt
    18661 tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    18721 ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    18781 tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat cgcgtacacg
    18841 gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    18901 gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc cggtcagcag
    18961 gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg ccgccatttc
    19021 aacatgcgag ttcacgccct gctcacgcag cacagcaact ttcggacgtg cgccagtggc
    19081 aatatacggt gctgccacat cttcgttgat atcgaacgac agtttcacat tcaggcccgg
    19141 atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt tgtcacgcag
    19201 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtggtgc ggctttcgct
    19261 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgccctac
    19321 ataatggaca caatcagcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    19381 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc
    19441 gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg ccatttccgc
    19501 cagcgttacc agcaggccgc catcagagcg gtcgtggtat gcgagcagct tacgctgtgc
    19561 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacgtcgc gtacatctgc
    19621 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    19681 gcctttgccc aaatcaatca gcagcagtgt gttatcttcg gtagaaagct gtggcgtgat
    19741 ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    19801 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    19861 caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt taacggcttc
    19921 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    19981 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    20041 cagacgggca gaggcggcga aatccagcag tgcaaccggc gcacgctcgc caatcgccat
    20101 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    20161 ctgccacggc cccaccatct gatcgcgcgc taccatgccg gttacgctgc ggtcgccgat
    20221 ggtcaccagg aaggttttct ctgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    20281 tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    20341 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    20401 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    20461 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    20521 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    20581 ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgcccgccgt cgctcaccag
    20641 ttccggcatc gcgttagaaa gaccgccagc gccgacgtcg tggataaaca ggattgggtt
    20701 ggcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct ccatctccgg
    20761 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgacctg acgccatgga
    20821 agacgccgct ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    20881 cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    20941 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc tgttcacttt
    21001 ttcttcataa gtacggaagt agccattcag tgccggacga ccaaattcgt tgttaaacgc
    21061 cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc gctcaggctt
    21121 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg aaacggagaa
    21181 accaaccaga ccagccttcg gcttcgcacc gcgtccggtg gcgccttcat cacgaatttc
    21241 gccgccggaa cccgtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    21301 ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc ccgtttcgtg
    21361 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    21421 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcttga acagcgattt
    21481 cggctgctgt tcaccgtcga taatccagtc ggcattaaaa attttgtggc ggcagtgctc
    21541 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    21601 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt
    21661 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgctggtaa ccggagtcgg
    21721 ttgatggtgg gcaaacaact gctctgcatc atctaaagca aaaaagaccg tttccatcat
    21781 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    21841 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    21901 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    21961 gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca ggcgttcaag
    22021 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    22081 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    22141 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    22201 gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    22261 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    22321 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    22381 ctttcactat tattacgttt tttcaag
//
LOCUS       DAZPLM010000054        22358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPLM010000054 DAZPLM010000000
VERSION     DAZPLM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.054"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1860)
                     /locus_tag="ACHVC7_001714"
     CDS             complement(<1..1860)
                     /locus_tag="ACHVC7_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2115794.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPASVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEED"
     gene            complement(1835..2380)
                     /locus_tag="ACHVC7_001715"
     CDS             complement(1835..2380)
                     /locus_tag="ACHVC7_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR2115795.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRLASEADLHPGTLEFERHRLTRAQATAQELKNA
                     KESAEVVETAFCTFVLSRIAREISSILDGIPLSVQRRFPELDNRHIDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            complement(2520..2744)
                     /locus_tag="ACHVC7_001716"
     CDS             complement(2520..2744)
                     /locus_tag="ACHVC7_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115796.1"
                     /translation="MIRGFVPHARQLCAGVEETGHVFRSVRPWSHDAGLSDIEFIANK
                     PERLRVLSGDPAGYGAEGARVFAIYENFPV"
     gene            complement(2978..3427)
                     /locus_tag="ACHVC7_001717"
     CDS             complement(2978..3427)
                     /locus_tag="ACHVC7_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115797.1"
                     /translation="MQEEAKKILVDLLNNASDGIDSAIAFSQAQIPDVVHQLLVWNIV
                     DSLIKTLIAISTIPLVIWFMKKQCKKVEIGKFDNDGRSCDNGQPKYKPTMIWESDGRL
                     SAFVLPLVAVFILWLSFIISIVANMTWLKIWLAPKLYLIEYAASLIK"
     gene            complement(3553..4413)
                     /locus_tag="ACHVC7_001718"
     CDS             complement(3553..4413)
                     /locus_tag="ACHVC7_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115798.1"
                     /translation="MKELQKINSIMERLIALELKLADCGKLCAEKQNELYREFVTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFTVKIQERVSGSVTREMAEQFVAMSNKTKRKKA
                     LLVTVNQLSGRVDFQVRQGGRVLVNDAFVGRCFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDGQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSATEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRIMPVPDVVIIDDIEKCKSVSSTATIKRILRCSTS
                     AVVFQKQLHN"
     gene            complement(4694..5005)
                     /locus_tag="ACHVC7_001719"
     CDS             complement(4694..5005)
                     /locus_tag="ACHVC7_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR2115799.1"
                     /translation="MKIYIAGPMTGYENFNRDAFNKEADRLSRHGHSVLNPATLPNGL
                     TQREYMDICFAMLRCADAILMLPGWKASAGATAEYHYAYKMEMPVFTTLNYPPACSSA
                     V"
     gene            complement(5047..5514)
                     /locus_tag="ACHVC7_001720"
     CDS             complement(5047..5514)
                     /locus_tag="ACHVC7_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2115800.1"
                     /translation="MSRVTAIISALVIGIIVCLSWAVNHYRDNAIAYKEQRDKNAREL
                     KQATATITDMQQRQRAADALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPA
                     SAKTSSASGMGNDATVELSSVAGRNVLGIRDGIIRDQTALRTLQEYINTQCLN"
     gene            complement(5511..6008)
                     /gene="rrrD"
                     /locus_tag="ACHVC7_001721"
     CDS             complement(5511..6008)
                     /gene="rrrD"
                     /locus_tag="ACHVC7_001721"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR2115801.1"
                     /translation="MQSSLRKAVTAAIGGGAIAIASMLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(6008..6280)
                     /locus_tag="ACHVC7_001722"
     CDS             complement(6008..6280)
                     /locus_tag="ACHVC7_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115802.1"
                     /translation="MKMDERYSSASYGSAGLAAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQMKRTKILQDILDKTNTNNLSATAMVIGELGKRAPKEI"
     gene            complement(6376..7452)
                     /locus_tag="ACHVC7_001723"
     CDS             complement(6376..7452)
                     /locus_tag="ACHVC7_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR2115803.1"
                     /translation="MFKTTNIHGAQLVCADSLQFIKTIPDNSVNLIATDPPYFGVKAN
                     AWDNQWDSDADFLGWIDEFLAEFWRILAPNGSLYMFTGSRRASKIELLTRDRFNVLNH
                     IIWAKPSGMWRGCHKESLRAYFPATERILFAEHYGASGYAKGQSGYASKCADLRKNIF
                     SPLIDAFSLARRQLDISAADINSATGKQMCSHWFSYSQWRLPSLIDFNKLCELFRRKA
                     DSLGVPCPYPFNVDYSEHEKRYSDLKLHYEEVKKQYDDLKAQYESLRRPFHVTADVPY
                     TDVWEFPPVQYYPGKHPCEKPAAMMEHIIKSSSRPGDIVADFFMGSGSTIKAALKLGR
                     QAIGIEIEGERYLQTVDEVKKLFE"
     gene            complement(7720..7947)
                     /locus_tag="ACHVC7_001724"
     CDS             complement(7720..7947)
                     /locus_tag="ACHVC7_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115804.1"
                     /translation="MNINEIRGNMTEAALSVECVMRGYPRISLKELSEACFLSQAAVE
                     FIIEQMICFGVAKRSGSGRYSLTDEYKQATF"
     gene            complement(7961..8182)
                     /locus_tag="ACHVC7_001725"
     CDS             complement(7961..8182)
                     /locus_tag="ACHVC7_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115805.1"
                     /translation="MNYQGNEKMRGDVAEIANELYELWQKVERFEKEYGFNSTNLTDR
                     LAGRLIGTMGPKLAELNRFMADVDFQFQD"
     gene            8346..8657
                     /locus_tag="ACHVC7_001726"
     CDS             8346..8657
                     /locus_tag="ACHVC7_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2115806.1"
                     /translation="MFNVITHPAALDELLELPDDLRGRMTRLIERLEKEGNKLKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPVKAIEIARTRLKEM
                     N"
     gene            8657..8944
                     /locus_tag="ACHVC7_001727"
     CDS             8657..8944
                     /locus_tag="ACHVC7_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2115807.1"
                     /translation="MKAKGIPFNEVKEKLLNTPEAIKGYEEADKELEMVEMLYEMRER
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLSKYAQACGATININAVY"
     gene            complement(8988..9191)
                     /locus_tag="ACHVC7_001728"
     CDS             complement(8988..9191)
                     /locus_tag="ACHVC7_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115808.1"
                     /translation="MTEASVSTLEELGSNLKDIDAVLDLVSVALASPEASLHLGEISR
                     LVSMSREIAQNCQQAIAVECLHH"
     gene            complement(9448..9774)
                     /locus_tag="ACHVC7_001729"
     CDS             complement(9448..9774)
                     /locus_tag="ACHVC7_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115809.1"
                     /translation="MALTAAERQRRRRERLKKEGTSRRDWILEPDELRMLGEICVLRR
                     PGRVPYSENEVIGLLIRKNYKELQKQLSTTCPRCGQKMPVSECIFDGEGSCHLTTTRL
                     KLVLKV"
     gene            complement(9790..10464)
                     /locus_tag="ACHVC7_001730"
     CDS             complement(9790..10464)
                     /locus_tag="ACHVC7_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR2115810.1"
                     /translation="MKFSKFSELVNRILLNRILSNNHSDRRDMDVTIVVHSPGSIGST
                     PSVEVQSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHK
                     EQLEKLSIELESAKNHIAELEAKPVSQTYKLTFEQWLEQQHDKIDVDCGCVSTEMLMH
                     WIRVAYEAGNSPVTPDGHIQFSVSLPAACGGDKYFIDGVFQPLRYERDCEKAVVAAGG
                     VVNWVK"
     gene            complement(10461..10751)
                     /locus_tag="ACHVC7_001731"
     CDS             complement(10461..10751)
                     /locus_tag="ACHVC7_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFR2115811.1"
                     /translation="MNIDTTITIDTALNTGLALLGWFYIMFSAGKWAASVFLKQWGKR
                     RKQEKRQKALEAFYDAFELSRIEPGTTAKIATKGDLMIVMFRQERAEKGESA"
     gene            complement(10751..11170)
                     /locus_tag="ACHVC7_001732"
     CDS             complement(10751..11170)
                     /locus_tag="ACHVC7_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2115812.1"
                     /translation="MAKAFTPEQREELNKQIVELVRLNGRGTVRQLADETGISRCAVS
                     RLSRELAASGDLYISGSGIFLSAQARKDWQNARKKLSRVKPKKSVVVDPDLIWSLPDG
                     EIRRYDRRLNMICHDCRKSEVMQRVLAFYQGNFQEVA"
     gene            complement(11170..11727)
                     /locus_tag="ACHVC7_001733"
     CDS             complement(11170..11727)
                     /locus_tag="ACHVC7_001733"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR2115813.1"
                     /translation="MIDLETMGTNTCAPIVSIGAVFFSPESEELGPTFYVPVNLRSSM
                     LNGGIADGDTILWWLKQSKEARAAICTNDALDIKDALFELSHFITCHACNLKKLKVWG
                     NGATFDNVILRGAYERVGLACPWEYFNDQDVRTIVNLGQFIGFNPKKDMPFDGERHNA
                     LADAIHQAKYVSAIFSRLVKGQGES"
     gene            complement(11793..11966)
                     /locus_tag="ACHVC7_001734"
     CDS             complement(11793..11966)
                     /locus_tag="ACHVC7_001734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115814.1"
                     /translation="MTHDEAERLSDTYRRRGKKVLVVRSDFLGDGYCVYVHLPESERT
                     PKPSRTYQQKIWV"
     gene            complement(12230..12940)
                     /locus_tag="ACHVC7_001735"
     CDS             complement(12230..12940)
                     /locus_tag="ACHVC7_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR2115815.1"
                     /translation="MELEYIRSCVSTALADVHYRQRGILEVQLEQMRLGRSGRFNNKP
                     VRSISVGDDNSYEVSVPAEPVRFHQGKTFKQSSMLLTDIDFQSASWRRAIGQLNNEES
                     AWLYYCYGCKPDYNNDVIVCQWLWLDFLVAHSGGGFKKMKASTKKAMRKLIYYAAQQV
                     KSELTCAEAVDEREQDRHLSFLLNISIDSWRQDYKERWLLIKSRCLSLNRTALLNAAE
                     KRSEIIKRHRAGSAILPL"
     gene            complement(12930..13151)
                     /locus_tag="ACHVC7_001736"
     CDS             complement(12930..13151)
                     /locus_tag="ACHVC7_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR2115816.1"
                     /translation="MKPEELIRHFGSVEKAAAGVGVTPGAVYQWLTAGEIPSLRQSDI
                     EVRTAYKLKSDFTTRRVKQSIKESRKRGA"
     gene            13243..13839
                     /locus_tag="ACHVC7_001737"
     CDS             13243..13839
                     /locus_tag="ACHVC7_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2115817.1"
                     /translation="MKTRGERLKARRLEMKLTLREVAEAVDISIAGVQNLERGDVMPS
                     LEIGIALAKCLRKPVHWVLYGTEYDPDRIPVIGTTESGPDEEWKPGQVPNTERFLPFI
                     SQRNTVYALTISNLTQGNYQPGDFLLVDSALELVPGEDVLVRDTAGNITIQRLARFDD
                     ENYYLDGTNSQRAIFRKSDLEFVHQIVGTIKSFMVEGR"
     gene            14085..18080
                     /locus_tag="ACHVC7_001738"
     CDS             14085..18080
                     /locus_tag="ACHVC7_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein, CyRepA1 family"
                     /protein_id="HFR2115818.1"
                     /translation="MTLNDFYADRFGSDPFSLIEAARDELTELAQMAGINWPACSDNI
                     QLNPRGGVERYSSYNKTSPEALEKSLKGRVEIYSRLEHSKGIDYPFINFVHKGSDAGS
                     WSGFSFLFSEYRREQQRNNATVVAQPEEERERIERQAEARRRREEQKRINDLKNNQMD
                     HERLLGWLAFHSAWEHAQAEDGSWPYAIKKGIRDVFSSCDVRRVTSHDSAKWSRGPTT
                     YMAIPLAHLDGRYDGRIVGWQRIDKQGGKYQTSAVTSGDFVGACFVIGELKGAQNIAV
                     TEGFATGASIWLATKKDKRFDAVVVAVSANNMVHVVEQLVNMYPAAKITCALDNDRKS
                     ATEGKGNTGLRTGYEILSKFNDVRCVYPTFEDDPQMECSDFNDLHKLRGLRETARQLF
                     ATANRLKTGTDLLSLTLNRLKTIKRDNRRTFAKELLRAVDIGMLTCPVPNSPSDLFNM
                     FCIVLREMGLENIYRATVKDHIARRLNKKCRIAQAPRSFSERITDPNKRPQHITYKRF
                     ETSVMTDDVLKYVQSLQGIVIVRAGMGSGKSTGLLRPLMHSSERGISVAHRVSLIGGL
                     WEMMTEGKGVKADILHYQDPGYQEMAPYASKLTICINSIVKNCWQPLMRQHDYFGFDE
                     ATQGLRAVLSGRAMENPVAVFNTLIDALARTEQHPIMVDADANDLLVDLAELAMKRRE
                     ELGLPAWLQIHVIELPVDVRNRETGEPIRVFYTEKDRIMTEVVKAVSDGEKIMLATDS
                     STFAEDVTATLRMHFPGKKFLCVNQKNKPEPEVEAFTNQPKKMVKKYDGLIYSPSISS
                     GVSIEQKHFDRHFGMFCGEVVPSDAVQMLRRDRTAKEFIIGFDKARAKRETDPQKIER
                     AFAQALLATAGMNGELTDVVFDGDRISMGVANTDFTRMKIKAAAIEATARNDYASNMI
                     CIMHSDGYKVSPLATDYAANDIGKDLRKEARDIVWEQTLDLHLNTETPQESEREELLK
                     KRALTLEEQAKLVRWDIENELKLPVEEGTLKFYFDGARDKVRRYETMLLDEVTARRYD
                     REEAAINFTYAYRQAGQWQYFVVTAMTREQADDVFKTKFPGIIDHKVKATPTVEVGAR
                     GFYALKSATLRQYFIDCGINPETMTGEATQERLKFARDNLMTAERRDLLNNVLHIGGF
                     MTPKGKPKVPEALFKNICDSLGLKTGKRRGRDGDKRPTLRFVEPESAAFMLDIIVQRQ
                     KDGLTLKTRKAEKTAIEMDRDLDLNIYMDDKTRSTNEQVQNAPHSVISEALAVLPVAV
                     PESWAMSALSSEELATMSSWSSASIAMTFASLYLTEFMDHLSSNELRRLREYITGNSS
                     CSYEEQGAFYG"
     gene            18269..18595
                     /locus_tag="ACHVC7_001739"
     CDS             18269..18595
                     /locus_tag="ACHVC7_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115819.1"
                     /translation="MSTTAFLDKLDYRQLQFCRDECERRIRAIQEEEKKVAWAVTDRG
                     LIFGWFRTEDYLKAVELLARKAAERWERADKENPETKYELNLYIEGNRLPLSEYNALF
                     ADGEWG"
     gene            18598..18993
                     /locus_tag="ACHVC7_001740"
     CDS             18598..18993
                     /locus_tag="ACHVC7_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115820.1"
                     /translation="MAVQSYKYESPLDKGYIRIKLSRYQHKAIFKVRKIRILDAYAYY
                     YNGENVVVEHFLARWFVALIFVPLLLIGTFVDGFPSTWREIKKGLFPRKYGRFSQDSW
                     RVIPGKHSVDEQPIVDFWLNRMADAKKVD"
     gene            19021..19308
                     /locus_tag="ACHVC7_001741"
     CDS             19021..19308
                     /locus_tag="ACHVC7_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115821.1"
                     /translation="MKSFIFVLCFAFSAAVYAQEDSISVINSSADLACKNNPDAASKE
                     SCKELLLSTAAFASENAKFYSAFCRGYVKDEDKEKCDDAGKLVRYFQSLNQ"
     gene            19685..19849
                     /locus_tag="ACHVC7_001742"
     CDS             19685..19849
                     /locus_tag="ACHVC7_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR2115822.1"
                     /translation="MFKFKFAAIVRTDKKSHIHHLSTIASSEREARRQFASRFVLVLS
                     ARIPVREVAA"
     gene            19846..20079
                     /locus_tag="ACHVC7_001743"
     CDS             19846..20079
                     /locus_tag="ACHVC7_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115823.1"
                     /translation="MNQVKMNTVGLLESLDERLAQVYALVSSAHRTISSCEASIYLSE
                     AGKLLQIACELTHEAQGYSASLSSQLLHDKEAE"
     gene            20076..20240
                     /locus_tag="ACHVC7_001744"
     CDS             20076..20240
                     /locus_tag="ACHVC7_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFR2115824.1"
                     /translation="MSALAVFSFQEEHQVRVVMINGEPWFVASDVCMAAGIDSTAIRK
                     LDEDEKGQAR"
     gene            complement(20286..22181)
                     /locus_tag="ACHVC7_001745"
     CDS             complement(20286..22181)
                     /locus_tag="ACHVC7_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="telomere resolvase"
                     /protein_id="HFR2115825.1"
                     /translation="MSKVKIGELINSLVSDVESIDNSDRPQGEKTKKIKAVALKFKNA
                     LFNDKRKFRGKGLEKRITANTFNAYMSRARKRFDDKLHHSFEKNIHRLSEKYPLYNEE
                     LLSWLSLPAADIRQRMSALQAKLKLILPLSEDLSNIRIGDNKSHNKLLKLSNKYKEWQ
                     FAISDLTSDNWKDKRDYLYKLLQQGSALLEELNQLKVNHEILYHLQLSPAERASIQQR
                     WADVLHNKKRNVVVIDYPKYMQAIYDIINSPATLNNLNTRSGMAPLAFALAALSGRRM
                     IEIMYQGTFTVSGKYTVDFLGQAKKRTSDDITRKIYTLCEAKTFVYLINVLRSCPAAS
                     DFDDVVKGYGDNDTRSENGRINAILAKAFNPWVKSFFKDDRRVYKDSRAIYARIAYEM
                     FFRVDPRWKNVDEDVFFMEILGHDDENTQLHYKQFKLANFSKTWRPNTGNENTRLEAL
                     QQLDDEMPGFARGDAGVRLHNTVKQLVEHDPSIKITNSTLRAFKFSPTMISRYLEFAA
                     DALGQFVGENGQWQLKIDSPEIVMPDEEELEAIPEQTDELSEDDDLDDDEIEIEEEEE
                     EESVEEQQPPAPSSPVSKPVFKPVRNIGDGSYVVEFHYNGQNYAWSGSANSPISAMQL
                     AWLTYFG"
BASE COUNT         6115 a         5341 c         4818 g         6084 t
ORIGIN      
        1 atcctcttcc tgcagactcg ccagcagcgc actgagatcc agctgccagc gggaaatact
       61 gatacgcagc gccgccagcg cataaacgaa gcagtcgagt gcttcattgc gtcgcttttt
      121 gctgtcccac agtatttttt tcctgccatc cacccatttt tcgacctgct cttcagccgt
      181 cagctgctgc gcttcggtca gatcaaaaat atccgggtta ttcgggaagt gaacggcacc
      241 gggaagcggt tcatcccctt ccggcgtcag tgtgaagcgg ttataaatct gctctttcgc
      301 ggtatccgta ccgatttcgg taaggtaaac cccgtttttg tttcgcttac gtggcatgct
      361 ggccaccggc tttccgtaga cggatgcccc tttaatgggg atcacccgga acagcccatg
      421 ttttttcgag cgttcataca caatggtcgg gtcaatcccg ccaatatccc agcagatacg
      481 ggatatcgac atttctgcac cattccggcg ggtataggtt ttattgatgg cctcatccac
      541 acgcagcagc gtctgttcat cgtcgtggcg gcccataata atctgccggt caatcagcca
      601 gctttcctca cccggccccc atccccatac gcgcatttcg tagcggtcca gctgggagtc
      661 gataccggcg gtcaggtaag ccacacgatc aggaacgggc gctgaataat gctctttccg
      721 ctctgccatc acttcagcat ccggacgttc gccgattttc gcttcccacg tctcaccgag
      781 cgtggtgttc acgaaggttt tacgttttcc cgtatcccct ttcgtcttca tccagtcttt
      841 gacaatctgc acccaggtgg tgaacgggct gtacgctgtc cagatgtgaa aggtcacact
      901 gtcaggcggt tcaatctctt caccggatga cgaaaaccag agaatgccat cacgggtcca
      961 gatcccggtc ttttcgcaga tataacgggc atcagtgaag tccagctcct gctgacggat
     1021 gacgcaggca ttatgctcgc agagataaaa cacgctggcg ggatcatccg gcgtccattt
     1081 gaggccaaac ggtgtctctt tgtcgccaaa tttaagatac tgctcctccc cgcagtgcgg
     1141 gcaggcaaca tgaaaacgca taaaatgcgg ggattcactg gctgcacgct caatctggca
     1201 ggttcctctc actttgggcg tggagccacg gatggacttt ggccagaccg agccttcaat
     1261 acgcttgtca cccaggaacg tcggagagcc ttcctgttca atatcatcat caaaagcagc
     1321 aagttcatca taacccgcca catccaccga cttttcacgg tagttttttg ccgctttacc
     1381 gcccaggcac cagaaaccac gaccattggt gaaacgcttc atggtgagcg tgttatcccg
     1441 gtgctttttg ccataccacg gggccagcgc cagcagcgac ggaatatcac ggatggtcgg
     1501 ctcgacgtgg gttttcataa agttttcggc atcaccatcc gtcggcaacc agataagggt
     1561 gttacgctgc ttatgctcta tgaagtaggc ataaacaccc agcagcattt tggaataacc
     1621 gacacgggca gacttcacca cattcacctc acggatgtag tcgctgccca tcgcattcat
     1681 gatggcccgc tgaaagggca gtgtttccca gcgcccttcc tggtatgcgg attctttcgg
     1741 gagatagtaa ttagcatccg cccattcaac ggcggtctgt ggctccggcc tgaacagggc
     1801 acgaagcccg gcgcggacaa aatgccgcag cctgtcaacc tgactgttcg atatattcac
     1861 tcagcaaccc cggtatcagt tcatccagcg cggctgcttt gttcatggct ttgatgatat
     1921 cccgtttcag gaaatcaata tgccggttat ccagctcagg aaaacgtcgc tgcaccgaca
     1981 gaggaatacc gtcgagaata ctggatattt cccttgctat acgcgacagc acgaaagtac
     2041 agaatgcggt ttccaccact tcagccgatt ctttggcatt tttcagttcc tgcgccgtcg
     2101 cctgagcacg agtcaggcga tggcgctcaa attcaagtgt tcctgggtga agatctgcct
     2161 cgctggccag ccgcagttct tcaacctccc ggcgcagctt ttcgttctca atttcagcat
     2221 ccctttcggc ataccatttt atgacggcgg cagaatcata aagcacctca ttacccttgc
     2281 caccgcctcg caaaacgggc attccctgct cctgccagtt ctgaatggta cggatactcg
     2341 caccgaaaat atcagccagc tgctttttgt tgacttccat tgttcattcc acggacaaaa
     2401 acagagaaag gaaacgacaa aggccaaaaa gcccgttttc agcatctgtc gtttcctttc
     2461 ttttcagggg gtgttttaaa taaaaacatg aagttacggc gaagaagaac ggaaacgcct
     2521 taaaccggaa aattttcata aatagcgaaa acccgcgcgc cttccgcccc gtagcctgcc
     2581 ggatcgccgg aaaggacccg caggcgttct ggtttattcg caatgaattc aatgtctgac
     2641 agaccagcgt cgtgcgacca cggtcgcacg ctcctgaata catgccctgt ttcttccacc
     2701 cccgcacaga gctggcgagc atgggggaca aacccgcgaa tcattagcgc tgtaaaaacc
     2761 cggtatgcat cgtttttgat tatttccaca cactcgcgca gaggagttcc ccgtcaggct
     2821 acggtcatag ttaatgcgga aatacggcga cgatgcagcg cataatgtgt caggcttgaa
     2881 tacctttatc cgttaaaagg gatattagtt aagttatccc gtgtagggta taagccatta
     2941 tcaaagccac tcagtagcga atggcttttg taatgggcta tttaattagt gatgctgcgt
     3001 attcgattag atataactta ggtgccagcc agatttttaa ccatgtcata ttggctacta
     3061 tagaaataat aaaacttaac catagaataa aaactgctac taatggtaag acaaaagcac
     3121 ttaacctacc gtcactttcc caaatcatag tcggtttgta tttaggctgt ccattatcac
     3181 atgagcgtcc gtcattatcg aacttaccaa tttcaacttt tttgcactgt ttcttcataa
     3241 accaaataac cagcgggatt gttgaaatgg ctattaatgt tttaatcaga ctatcaacta
     3301 tattccatac cagcaactga tgaacaacat ccggaatctg agcctgactg aatgcaatgg
     3361 cggaatcaat tccatcgctg gcattgttca ataaatcaac aagaattttc tttgcttctt
     3421 cttgcatgta tatatcctgt aagttgtaag cctgttcgaa aaaataaaca actacgagag
     3481 acagaaaaaa aaatatctca ggctctcttt ctataagctt ttagttgagg atgttctgca
     3541 aaacaaaact ggttagttat gtaactgctt ctggaataca acagccgacg tgctacaacg
     3601 taaaattcgt ttgattgtgg cagttgagga aacagacttg catttctcaa tatcatcaat
     3661 gatgaccaca tccggtacag gcatgatgcg ggtatgcaat gtttttgcac taataacatc
     3721 acagccattc agtggacgaa tatctgcatc ttcaggcact accagaagca cacgtttctc
     3781 cgttgcagaa aagccagcag caagagttcg cgacaataaa gtcattccac tacgtggctt
     3841 catacaaatt tcagaaatat gatttttttc gtccaaatga tgctggccat catttttatc
     3901 atcactcaac aactctgctg ttaatacaat ccctgctgtc tcagatttcc aggaaatgct
     3961 gatatcctga tcatctgcat caacatcata tttcatcgag taaggactaa agcaacgtcc
     4021 aacgaacgca tcgtttacca gtacacgccc accctgtctg acctgaaagt caacgcgccc
     4081 tgaaagctga tttactgtta caagcaatgc cttttttctt tttgttttat tactcattgc
     4141 cacaaactgt tcagccatct ctcttgtcac actaccagaa actctttctt gaattttcac
     4201 agtaaatttt ctggatgtag tagcatcgac attaaaaaat ctggatgtca ttaggcagtt
     4261 attaacaatt ggcatcagct cacttacgat tgtaacaaat tcccggtaaa gctcattttg
     4321 cttttcagca cacagttttc cgcaatcggc cagcttaagc tcaagagcaa ttaatcgttc
     4381 cattattgaa ttaatttttt gcaactcttt catcaaaacc tcctgacaaa gataccgtgc
     4441 gaccacggtc gcacgctcct gaatacatgc cctgtttctt ccaccctcgc acaggactag
     4501 cgagcatgag ggacaaaccc gcgaatcatt agcgcggtaa aaacccggtg tgcatcgttt
     4561 ttgattattt ccgcacactc gcgcagagga gttccccgtc gggctacggt catagttaat
     4621 gcgggaatac ggcgacgatg cagcgcggaa tttagcgagc gaccacgatc gcacacgaga
     4681 aacaaagaga tttttatact gcagaggaac aagctggcgg gtaattcagc gtagtgaata
     4741 ccggcatctc catcttgtat gcgtaatgat actcagcagt tgctccagca gacgctttcc
     4801 agccaggaag catcaaaata gcatccgcac aacgaagcat tgcaaagcaa atatccatgt
     4861 attcacgttg tgtcagacca ttaggcaaag tggctggatt caagacagag tgaccatgtc
     4921 gtgacaaacg atctgcctct ttattaaagg cgtcacgatt aaagttctca tatcccgtca
     4981 ttggcccggc aatataaatt ttcattcact atccaacaaa ttgagttcgt ttttgataca
     5041 gagggattaa ttcaggcatt gcgtattgat gtattcctga agcgttctca gtgctgtttg
     5101 gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg aagagagttc
     5161 gacggtggca tcattgccca tgccggaggc gctggaggtt ttggctgagg ctggcacagg
     5221 gcattttcct ttgacgagca cccgaccacc attatcaagc ttacgccgaa gagcatcatt
     5281 ttcagctttc gcattggcta actccttcgt gtatttagca tcgagtgcat cagcagcacg
     5341 ctggcgctgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctctc tggcgttttt
     5401 gtcgcgctgc tctttgtagg cgatggcgtt atcacggtaa tgattaacag cccacgacaa
     5461 gcagacgatg atgccgataa ccagagcgga gataatcgcg gttaccctgc tcattgttga
     5521 ccccacaaac agacttcacg ctcaatctca cgacgagtca tcaggccttt ccattgctta
     5581 ccgccagcat atatccagcg acgtagctga tcacatgcgc ctttgatatc gccctggttt
     5641 attttgcgaa gaagcgtcga tgttctgaaa ttaccagcgc ccacgttgta aacgaacgag
     5701 taaagagcgc cgcgcgttgt ttccggtata tcgactttga tgtacggatt aatttgtctg
     5761 gcaacagtgg caaggtcttt attcaggaga gctttgcatt ctgcttcggt atacgtttta
     5821 ccgagcatga tgtcttttcc agtgtgcccg taacatacag tccatacgcc aacaatatct
     5881 ttgtatggta tgtaactgac accttccaga ccatcgttac cacttgggcc agtgatgagc
     5941 atagacgcta tggcaatagc cccgccacca atagcagctg tgacagcttt gcgtaatgat
     6001 gactgcatca tatttccttc ggtgctctct tccccagctc tccaataacc atcgctgtag
     6061 cagaaagatt attggtatta gttttatcca atatgtcctg caatatcttc gtgcgcttca
     6121 tctgctcgcg tttattgagc cggtatgtaa gcacaccgag gataatgctg aacgcgacac
     6181 cgataatgaa gccccaatcc tgcaatgaca ggctggcaaa gaacgcagca agaccagcgc
     6241 taccataaga tgcactgctg tatcgttcat ccatcttcat atctcttacc tcgcaatagt
     6301 tgctgaggtt tcggcttgga aactatatgg tgaaaataac ccctaccaaa aacggcaggg
     6361 gaatatattc tccagttact caaataactt tttcacttca tcaactgtct gaagataacg
     6421 ctcgccctca atctcaatac ctattgcctg acgtcctaac ttcagcgcag ctttaatagt
     6481 tgagcctgat cccataaaga aatcggcaac gatatctccg gggcgtgaac tactctttat
     6541 aatatgttcc atcatcgctg caggtttttc acatgggtgc ttgccaggat aatattgcac
     6601 aggaggaaac tcccatacat cggtataagg tacatcagca gtaacatgaa atggacggcg
     6661 taaactttca tactgagcct tcaaatcatc atactgcttc tttacttcct cataatgcaa
     6721 tttcagatcg ctataacgct tttcatgttc tgaataatca acattaaaag gatatggaca
     6781 cgggacaccg agtgaatctg ccttcctgcg aaataattcg catagtttat taaaatcaat
     6841 taatgatgga agccgccact gcgaataaga aaaccagtgt gaacacatct gctttcctgt
     6901 cgctgaatta atgtctgcgg ctgatatatc taactgacgg cgagccagcg aaaaagcatc
     6961 aattagtgga gaaaaaatat tttttcttaa atctgcacat tttgaagcat aacctgactg
     7021 acctttagca taacctgacg cgccgtaatg ctctgcaaat aatattcttt cagtagcagg
     7081 aaaataagcc cttaaacttt ctttatgaca accgcgccac ataccactgg gtttagccca
     7141 aatgatatgg ttcagaacat tgaaacggtc gcgagttaat aattcaattt ttgacgcacg
     7201 gcgagagccg gtaaacatat acaggctgcc attaggggcc aatatccgcc aaaattctgc
     7261 caaaaattcg tcaatccacc ccaaaaagtc agcatcgcta tcccactggt tatcccatgc
     7321 gttagccttt acgccgaagt atggtgggtc tgttgcaatc aaattgaccg agttatcagg
     7381 gatggttttg ataaattgca gagaatctgc gcaaacgagt tgtgcgccat gaatattagt
     7441 ggttttgaac atagctatta tttttttgcc tgggtaagct aacccagcga tgcgcatcgc
     7501 gggtgggctt taggttcagc ctatagctct ggcatgggtt gaccgcagga caagctgcaa
     7561 cttgtcctgc gcccactttt caggcacaaa aaaaccgcca taaaggcggc ttgttggcta
     7621 ttgggtacaa aaaacccaac ttaacaaaac aataactaaa aaccgtcatt ttgccaagct
     7681 ttttcgtttt tgtgctgtgc gaccgtggtc gcacagtttt tagaaagttg cctgcttata
     7741 ttcatcggtc aaagaatatc gaccagaccc gctacgcttt gcaaccccaa aacagatcat
     7801 ctgttcgatg ataaattcaa cggcagcctg actcaaaaaa caggcttcgc ttaactcttt
     7861 caaggaaatg cgtggatatc cacgcataac acattccaca ctcagggccg cttcggtcat
     7921 atttccacga atttcattaa tattcataac gcctctctaa tcaatcttga aactggaaat
     7981 caacatcagc cataaagcgg ttcaactcag ccagttttgg ccccatagtc ccaattaagc
     8041 ggccagccag tcggtctgtc aggttggtac tgttgaaacc atattccttt tcgaaacgtt
     8101 caaccttctg ccacaactca tacaattcgt tagctatctc cgcaacgtcg ccgcgcattt
     8161 tttcgttacc ttgataattc ataatatcct cgaaattcat atagttagtg ggttatcccc
     8221 ctcaacaaca cgaactgtaa ctctgccaaa agaacacatc cagtgttatt ttcacttttt
     8281 agtgaaattt tatatttgct ttgttttctc gttttggtgt attttgcgcg ttaggaggct
     8341 ataatatgtt taacgtgata acccaccctg cagcactgga tgaactgctt gaactaccag
     8401 acgatctgcg aggtcgcatg accaggctaa ttgaacgact ggagaaagag ggaaacaaac
     8461 ttaagatgcc ccatagccgt gtaataggcg gaggactctt tgaactaaga gtaggggata
     8521 aaaacatcgc aagaacgcta tacgcctacg cgactggtaa cgaaatttat ctattgcatg
     8581 catttgtaaa aaagacacag aaaacaccag taaaggctat agaaatcgcc agaactcgct
     8641 tgaaggagat gaactgatga aagcaaaagg catcccgttt aacgaagtaa aggaaaaact
     8701 actcaacact ccagaagcta tcaaaggata tgaagaggcc gataaagaac tggagatggt
     8761 cgaaatgtta tacgaaatga gggaaagagc aggtttaacg aagtcagctc tggcagaacg
     8821 aatgggattg cgcccgtccg ccatcagccg cctggaaagc aatcctttag gggcaagcat
     8881 gaaaacttta tcaaaatatg cccaagcatg tggcgcaaca attaatatta atgccgtcta
     8941 ttgattaatc acttttcacc accccgtcca gaagggcggg gtgtgtatca atgatgtaaa
     9001 cactccacag caatcgcttg ttggcaattc tgagcaatct cccgcgacat actaacaagc
     9061 cgtgaaatct cgccaagatg caagctagct tcgggagaag ccagagcaac gctaaccaga
     9121 tccaaaacgg cgtcgatgtc ttttaggttt gatccgagtt cttcaagagt tgatacagat
     9181 gcttcagtca ttgaatctaa taccatttaa ttaaaaaagg ttatttgata ctgtataaaa
     9241 ttacagggta aaactcaaat tttatagcca actttcaatg gattaacagg gttaatagct
     9301 ttgtagtccg atacttatgt tctcttccta cgcctcactc aaccacctac agaagcccat
     9361 catcaccata agaaaaactc ttttcgcttc gttttctcat catttcaaaa actatgcctt
     9421 tttgtttatt tatcattccg tgaccagtca cactttgagc acaagtttca gcctcgtagt
     9481 tgtaaggtga catgagcctt caccatcaaa aatacattct gaaacaggca ttttctgacc
     9541 acatcgcggg caagtagtag atagctgctt ctgcagctcc ttatagtttt tcctgatcag
     9601 cagaccgatg acttcattct ctgaatatgg aactcgtccc ggtctacgca atacacaaat
     9661 ttcccccaac atacgtagct cgtcaggttc cagaatccag tcccgtcttg atgtgccttc
     9721 cttcttcaac cgttccctgc gtctcctttg ccgttctgca gctgtcagcg ccataaatcc
     9781 tcctgaaaat tacttaaccc aattaactac accgccagcg gccacaaccg ctttttcaca
     9841 gtcacgctca tatctcaaag gttgaaatac accatcaata aaatatttat ctccaccaca
     9901 tgctgcagga agtgaaacag aaaactgaat atgaccatcc ggagttaccg gagagttgcc
     9961 agcctcatac gcgaccctta tccagtgcat aagcatttct gtacttacac atccacaatc
    10021 aacatcaatt ttgtcatgct gctgttccag ccattgctca aaagttaact tgtaagtttg
    10081 gcttacaggt ttggcttcca gttctgctat gtgatttttg gctgattcca actcaatcga
    10141 caatttttcc aactgctctt tatgcttctt gtattcctga tatgcgtgcc aagactgacc
    10201 tttgcgcaca ctatccgtga tatcagtaat ctgttctggt gttagcgtgg tcagtggctg
    10261 tgctgggaaa atcagcactt tcccggaatc ccaatcaaaa ccagcgtgaa ttgactgaac
    10321 ctcaactgaa ggtgttgaac caatgctgcc aggcgaatga acaacgatcg ttacatccat
    10381 atcgcgacga tcgctgtggt tgttggacaa aatacgattc aacaaaatac gattcaccaa
    10441 ctcagaaaat ttagaaaatt tcatgctgat tcacctttct ctgctctctc ctgtcggaac
    10501 atcactatca tcaggtcgcc ttttgtcgct atcttggctg ttgtgcctgg ctcaatgcgg
    10561 ctaagttcaa atgcgtcata gaacgcctcc aacgctttct ggcgtttttc ctgtttgcgg
    10621 cgctttcccc actgctttag aaaaacagag gccgcccact tccccgcact gaacatgatg
    10681 taaaaccaac cgagaagtgc cagaccggta tttagggccg tatcgatcgt tatcgtggtg
    10741 tcgatattca ctacgccacc tcctgaaaat ttccctgata aaacgccaac acacgctgca
    10801 taacttcgct cttccgacaa tcgtgacaaa tcatgttcaa gcgcctgtca taacgacgta
    10861 tttctccatc aggtaatgac cagataaggt caggatcaac aactaccgat ttcttcggct
    10921 ttactcttga tagtttttta cgggcgtttt gccagtcctt gcgtgcttgt gcagacagaa
    10981 atatcccgga gccagagata tacaaatcac cactggcagc aagctctctt gataaacgac
    11041 taacagcaca ccggctaata ccagtttcat ccgcaagttg cctaaccgtt cctcgtccat
    11101 tcaggcgcac gagttccaca atttgtttat tcagttcttc cctctgttct ggtgtaaaag
    11161 cctttgccat tacgattctc cttgcccttt tacaagacgg gagaaaattg cggatacata
    11221 tttagcctga tgaatagcat cagccagggc attgtgtcgt tcgccatcaa atggcatatc
    11281 ttttttagga ttaaaaccaa taaactgacc aagattaacg attgttcgaa catcctgatc
    11341 attaaaatat tcccacggac aggctaagcc aacgcgctca taagcaccgc gtaaaattac
    11401 attatcgaac gttgctccat ttccccatac tttcaacttt tttaaattac atgcatggca
    11461 agtaataaag tgtgataatt caaaaagtgc atccttaata tcaagggcat cattagtaca
    11521 aatagcggct ctggcttcct tgctctgttt caaccaccat aaaatggtat caccatcggc
    11581 gataccacca ttcaacatcg agcttctgag attcactggc acataaaaag taggacctaa
    11641 ttcctcactt tctggagaaa aaaacactgc cccgatagaa acaattggag cacatgtgtt
    11701 agtacccatt gtttcaaggt caatcattac atcagaccgt ttcctgtcgt aaataatctg
    11761 attagtcacg aataattctc ctcaacgttt atctataccc aaattttctg ttgatatgtt
    11821 ctggatggtt ttggcgttct ttctgattca ggtaaatgaa cataaacgca atatccatca
    11881 cctaaaaaat cagaacgaac aaccagaact ttcttaccac gccgccgata agtatctgaa
    11941 agacgctctg cttcatcatg cgtcatcggc ccctgcttaa atggcgttat tttcattttc
    12001 ttgtgcgacc acggtcgcac cccctctgat ttccaagtaa cgcttaatcc aaagattttc
    12061 tatatgctta ttacctggct gatttgataa ataccaatcg gcaattacag actgcttatt
    12121 actatctggt cgtgtcctgt aaccacaagt tggacaccaa ataacatatt catttcttac
    12181 ccccgaatac cttaattctg gcttcgacgg tttcctgaac ataacttgtt tacaaaggca
    12241 gaatggcact tcctgcccga tggcgtttga tgatttcact gcgcttctcc gccgcattta
    12301 ataatgcggt tcgatttaag ctcagacatc gcgacttaat taaaagccag cgttctttat
    12361 aatcttgtct ccagctatca atagatatat tcagtagaaa actcagatgc ctgtcctgtt
    12421 ctctctcatc aactgcctca gcacacgtaa gttctgattt aacctgttgt gccgcgtaat
    12481 aaatcaattt ccgcatggct tttttcgtgg aggccttcat ttttttaaag ccgccccccg
    12541 aatgagcaac cagaaaatca agccataacc actgacaaac aatcacatca ttgttgtaat
    12601 caggcttgca tccgtagcaa taataaagcc aggctgattc ctcgttattc aactgaccaa
    12661 tagccctgcg ccagctggcg ctctgaaaat caatatcagt gagcaacatt gatgattgtt
    12721 taaatgtttt cccctgatgg aatctaaccg gctcagcagg aacggacact tcgtatgagt
    12781 tatcatcccc cacactgatt gaccggactg gtttattgtt aaaccgtcct gatctaccga
    12841 gacgcatctg ctcaagctgg acctccagaa tgccacgctg gcgataatga acatcagcca
    12901 atgccgtaga gacgcagctg cgtatatact caagctccac gcttacgact ctccttaatc
    12961 gactgcttta cccgccgagt tgtaaagtca ctcttcagct tataagcagt acgaacttca
    13021 atatcgcttt gccgcaatga aggaatctct ccagcggtta accattggta aacagctccg
    13081 ggtgttacac ctacccctgc cgctgctttt tctacactgc caaaatggcg aataagttct
    13141 tctggtttca tgttgttatt ataaacaata aacaacaatt aaagccagct ataataaata
    13201 aattttatag ccagctataa gaagatcatt tatgattaca gtatgaaaac acgaggcgaa
    13261 cgactgaaag cacgccgttt agagatgaag ctgacgttgc gagaagttgc tgaggctgtc
    13321 gatatctcta tagcaggcgt ccaaaactta gaacgtggcg acgtaatgcc gtcactggag
    13381 ataggtattg ccctggctaa gtgcttacgc aagccagtgc attgggtgct atatgggaca
    13441 gaatatgacc ctgatcgcat tcctgttatt ggcacaactg aaagtgggcc ggatgaagag
    13501 tggaagccgg gacaggttcc aaatactgag cgattcctgc cattcatcag ccagcgcaat
    13561 actgtgtatg cgttaactat cagcaatctt acgcagggaa actatcagcc aggcgatttt
    13621 ctcctggtcg attctgcgct ggaactggtc cccggcgagg atgttctggt tcgtgatact
    13681 gcaggaaata tcactatcca gcgcctggcc cgttttgatg atgaaaatta ctatctggat
    13741 ggaacaaact cacaacgcgc catcttcagg aaaagcgatc ttgaatttgt tcaccaaata
    13801 gtcggaacga tcaaatcatt catggttgag ggtagatgac ttaatacggg gtttattgtt
    13861 tgctgtaaat ctggtttaat gcagggcata tgttctgtgg gttgactcag gttacagcag
    13921 cagaaaaaag acgaaaaaaa acccgagtcg gcaaactcgg gtccttttca ggaagcagcc
    13981 acagtaacgc gacggattcc gtcaattcaa gatgcgttta ttgtggctgc tcctgcggat
    14041 tttttcaacc cgaaaaaaca taattcgcat ggaatggcta aaaaatgaca ttaaacgatt
    14101 tctatgcgga tcgctttggc agcgatccgt tctcgctaat tgaagcagca cgggatgaac
    14161 taaccgagct ggcacagatg gctggtatca actggcctgc ctgttccgac aacatccaac
    14221 tcaaccctcg cgggggggtt gagcgttact cttcatacaa taaaacatct ccagaagctc
    14281 tcgaaaagag cctgaaagga cgcgtagaaa tttactccag gctggaacac agcaaaggga
    14341 ttgattaccc gttcattaac ttcgtccata aaggcagcga tgctggctca tggagcggtt
    14401 tctccttcct gttttctgaa tatcgccgtg agcaacaacg gaataatgcg accgtggtcg
    14461 cacaaccaga agaagaacgg gaaagaatag aacgtcaggc cgaagcacgt cgtcgccgtg
    14521 aagaacaaaa acgaattaat gacctgaaaa acaaccaaat ggaccatgaa cgtttgctgg
    14581 gatggctggc attccacagc gcgtgggaac atgctcaggc tgaagacggc tcatggccat
    14641 atgcgattaa aaaaggtatt cgtgacgttt ttagttcatg tgatgtgcgt cgcgtgacca
    14701 gtcacgacag tgcgaaatgg agccgggggc caaccacata tatggctatc cctctggctc
    14761 acctggacgg tcgttatgat ggtcgtattg tgggttggca gcgaatcgac aaacaaggtg
    14821 ggaaatacca gaccagcgca gtaaccagcg gtgatttcgt cggtgcctgc tttgttatcg
    14881 gtgagctgaa aggggcgcaa aacatcgctg ttacagaagg ttttgctact ggtgcatcca
    14941 tctggcttgc cacgaaaaaa gataaacgct ttgacgctgt ggttgtcgct gtatccgcta
    15001 acaacatggt tcatgttgtg gaacaactgg taaacatgta cccggcggca aaaatcacct
    15061 gcgcactgga taatgaccgg aaatctgcca cagaaggaaa agggaatacc ggcctgcgga
    15121 ctggctatga aatcttatcg aaattcaacg atgtccgctg cgtctatcca acttttgaag
    15181 acgatccgca aatggagtgc agtgacttca acgacctgca caaactacgt ggacttcgtg
    15241 aaacagcacg tcaactattt gcaacagcaa accgcctgaa aaccggtact gatttgctat
    15301 ctctgacgct caaccgcctg aagacaatta agcgggataa ccgtcgtact tttgccaaag
    15361 agctgctgag agcggtggat attggcatgc taacctgtcc ggttcctaac agtccgtcag
    15421 atttatttaa catgttctgc atcgttctgc gtgaaatggg actggaaaat atctatcgcg
    15481 ctacggtaaa agaccacatt gcccgtcgcc tgaataaaaa gtgccgtatt gcccaggctc
    15541 cgcgctcttt cagcgaacgc ataactgatc ccaataaacg ccctcaacat atcacctata
    15601 agcgttttga aacttcggta atgaccgatg atgtcctgaa gtacgtgcaa tcactacagg
    15661 ggatagtcat cgtccgtgct ggtatgggtt ccggcaaatc tactggattg ttacgaccgt
    15721 taatgcatag ctcagaacgt ggtatctctg ttgctcaccg tgtctccctt attggtggcc
    15781 tgtgggaaat gatgacagaa ggaaaaggtg taaaagccga tatcctgcac tatcaggacc
    15841 ctggctatca ggaaatggct ccatacgcca gtaaactgac catctgcata aactcgattg
    15901 ttaaaaactg ctggcaaccg ttgatgcgcc agcacgacta ttttggcttt gacgaggcca
    15961 cacaagggct acgtgccgtt ctttccggtc gggctatgga aaacccggtt gctgtattca
    16021 atacgctaat tgacgcgctg gccagaacgg aacaacaccc aatcatggtg gatgctgatg
    16081 cgaacgatct actggttgac ctggctgaac tggctatgaa acgccgtgaa gaactaggtt
    16141 taccggcatg gctgcagatc cacgtaattg aattacctgt tgatgttcgc aaccgcgaaa
    16201 caggtgagcc aatccgggtt ttttatacag aaaaagatcg catcatgacc gaggtggtga
    16261 aagctgtatc ggatggcgaa aaaatcatgt tggccaccga cagctccacc tttgcagaag
    16321 atgtgacagc caccctgaga atgcattttc ctggtaagaa atttctgtgt gtaaaccaga
    16381 aaaacaaacc agagccagaa gttgaagcat ttaccaatca accgaaaaag atggtcaaaa
    16441 aatacgacgg cctgatttat agcccgtcta tttcatccgg cgtgtcgatt gagcaaaaac
    16501 attttgaccg tcatttcggc atgttctgtg gtgaagttgt acccagcgac gcagtgcaaa
    16561 tgctacgccg cgaccgtaca gcaaaagaat ttattatcgg ctttgataaa gctcgtgcaa
    16621 aacgtgaaac tgacccgcaa aaaatagaac gagcattcgc ccaggcattg cttgctaccg
    16681 caggtatgaa cggtgaactt acggatgtcg ttttcgatgg cgaccgtata tcaatggggg
    16741 tggccaatac cgactttacc aggatgaaaa ttaaagcagc cgcaattgaa gcgactgcgc
    16801 gtaacgacta cgccagcaac atgatctgca tcatgcacag cgacggttat aaggtatcac
    16861 cgctggccac cgattatgct gcgaatgata tcggtaaaga tctgcgtaaa gaagcgcggg
    16921 atattgtgtg ggaacagaca ctggatctgc acctcaatac agaaactcca caagaatcag
    16981 agcgtgaaga actgctaaaa aaacgcgcgc tgacgcttga agaacaggca aaacttgtcc
    17041 gctgggatat tgaaaacgaa ctgaaactac cggttgaaga aggcacgctg aaattctatt
    17101 tcgatggtgc tcgagacaaa gttcgccgct atgaaacaat gcttttagat gaggtaacag
    17161 ctcgtcgtta cgaccgggaa gaagcagcca tcaattttac ctacgcatac cgtcaggcgg
    17221 gacagtggca atattttgta gtgaccgcga tgactcgcga gcaggctgat gatgtcttta
    17281 aaactaaatt ccctggaata atcgatcaca aggtaaaagc tactcctact gttgaagtcg
    17341 gggcgcgcgg tttctatgcg cttaaatcag caactctccg gcagtacttt atcgactgcg
    17401 gtatcaaccc tgagactatg accggcgagg cgactcagga gcgtctcaaa ttcgctcgag
    17461 ataacctcat gacagccgaa cgccgtgacc tgttgaataa cgttctgcac attggtggtt
    17521 ttatgacgcc aaaagggaag cctaaagtgc cggaagcgtt gtttaaaaac atttgcgact
    17581 ctcttggcct taaaacaggt aaacgtcgcg gcagagacgg cgataagcgt ccaaccttgc
    17641 gttttgttga gcctgaatca gcggcattta tgctggatat tattgttcag cgtcagaaag
    17701 atggcctaac cctaaaaacg cgtaaagccg agaagacagc catcgaaatg gatcgcgatt
    17761 tggatctcaa tatatatatg gatgataaaa cgcgatccac aaacgagcag gttcaaaacg
    17821 cccctcattc agtaatttct gaggctttgg ccgtgttgcc agtggctgta cctgagtcct
    17881 gggcgatgag tgcgctttcc agtgaggagt tggccactat gtcatcatgg tcatcagcca
    17941 gcatagctat gacgtttgca tccctgtacc tcacagagtt catggatcac ctttccagca
    18001 atgagctgcg gcggttacgt gaatacatta cggggaatag ctcatgcagc tacgaagagc
    18061 agggcgcttt ctatgggtaa tgagtggata ttatctttgt gtctcttgct ttgtgtgttc
    18121 cagcaatggt tttggttcat agaccgaaag tctcacaagg aaaacatgaa ggccaggaag
    18181 gcgatgtttc gtggtgaaca atttgcgcag acttatcgta tgtggaaacg tttctatcgt
    18241 gaattttcgg tgaacgggga gtaaaaatat gtctactact gcatttttgg acaaacttga
    18301 ttaccgtcag ctgcagttct gccgggatga atgcgaacga cgcattaggg ctatccagga
    18361 ggaagaaaaa aaggtcgcct gggctgtaac agatcgtggc cttatctttg gctggttccg
    18421 tacggaggat tatctgaaag cagtggaatt attggcgcgt aaggctgctg agcgttggga
    18481 acgagccgac aaggaaaacc cggagacgaa gtatgaactg aatctctaca tcgagggcaa
    18541 ccgtctacca ttgtcagagt ataacgcttt gtttgctgat ggtgagtggg gttaaaaatg
    18601 gctgttcaat catacaaata cgaatcccct ttggataagg gatacattcg tatcaagtta
    18661 agtcgttatc agcataaggc tatttttaag gtaaggaaaa ttcgcatatt ggatgcgtat
    18721 gcgtattact acaacggtga aaatgttgtt gttgaacact ttcttgcacg atggtttgta
    18781 gctctgattt ttgtccctct cctgttaatt ggtacttttg tggatggttt tcctagtaca
    18841 tggcgggaga taaagaaagg cctattcccg cgtaagtacg gcagattttc gcaagatagc
    18901 tggcgagtta tacctggtaa acattctgta gatgagcaac cgattgttga tttctggttg
    18961 aatcgtatgg ctgatgctaa aaaagttgat tgaattgtga tgactgttac aggagataaa
    19021 gtgaagagtt ttatttttgt tttatgtttt gccttttctg ctgcggttta tgcccaggaa
    19081 gatagcattt ctgttattaa ctcatcagcg gaccttgcat gtaaaaataa tcccgatgct
    19141 gcatcaaaag agtcatgcaa agagctgctc ctttcgacgg ctgcttttgc ttctgaaaat
    19201 gcaaaatttt attcagcctt ttgtcgcggc tatgttaagg acgaagataa agaaaaatgt
    19261 gatgatgccg gtaagttagt taggtatttc caatctctta atcaataatg gcctttgatt
    19321 aacgatcaac ttctgatcac tcgttcaagc aaactataaa aacacctgtc cccgtgactg
    19381 gtcacggggt aaaatgtctt tgcaaattat tgacgtgcgc gtcgttttga cggtaaagtt
    19441 tactccgcta cggtaaatcc cgtagccggg cgtaggaacc cgagtaaatg aagcgcacaa
    19501 cacgcgccag cgtgtttttt tgtgtgtaaa atctgcgtac accaaattta tggtggctca
    19561 gatggggcag cttttagctg gccggtttct tcgtttaccg gtattcctac ccccgtctgg
    19621 gctaccgcca tatagagcgt aggaactctg gtggtagcac gaccaattaa acggagtgca
    19681 taccatgttc aaattcaaat ttgcggcaat cgttcgcaca gacaaaaagt cacacatcca
    19741 tcatctttcc accatcgcgt catctgaacg ggaagctcgt cgccagttcg ccagccggtt
    19801 tgtcctggta ttgtctgccc gtattccggt gcgcgaggtg gctgcatgaa tcaggtaaaa
    19861 atgaacactg ttggcctgct ggaatcgttg gatgaacgtc tggctcaggt ctatgcgctc
    19921 gtatcatcag cccatcggac aatctccagt tgtgaggcgt cgatttatct ctcagaagca
    19981 gggaaactcc ttcaaattgc gtgtgagcta acacacgaag cacagggtta ttcagcatcg
    20041 ctctcgtcgc aattgctgca cgataaggag gctgaatgag cgcgttagca gtcttttcct
    20101 ttcaggaaga gcatcaggtt cgtgtagtga tgattaacgg cgaaccgtgg tttgttgcat
    20161 ctgatgtgtg tatggcggcg ggtattgatt ctacggccat cagaaagttg gatgaggacg
    20221 aaaaggggca ggcgaggtag accacacaac aaggcaccga taattcggtg cctttaccgt
    20281 atgctttatc cgaaatatgt cagccatgct aactgcattg ctgatatagg gctgtttgca
    20341 ctgccggacc aagcataatt ctggccatta taatgaaact caacaacgta tgagccatca
    20401 ccaatatttc tcacaggctt aaataccggc ttagatacag gtgaagaagg cgcgggcggt
    20461 tgctgttctt ctacgctctc ttcctcttcc tcttcctcta tctcaatttc gtcgtcatcc
    20521 agatcgtcat cctctgataa ttcatccgtc tgttcaggta ttgcttccag ctcttcttcg
    20581 tcaggcataa cgatttctgg agaatctatt ttcagctgcc attgtccatt ttcgccaaca
    20641 aattgcccca gagcatcagc agcaaattcc aggtatcgac taatcattgt agggctaaat
    20701 ttgaatgctc ttagagtgct gttcgtgatt tttatagagg ggtcatgctc taccagctgc
    20761 ttaactgtgt tatgcagacg aaccccggca tctcctctgg cgaatcctgg catttcgtca
    20821 tcaagctgct gcaatgcttc cagcctggta ttttcgtttc cagtatttgg cctccatgtc
    20881 tttgagaagt tggccagctt gaattgctta tagtgtaatt gagtgttttc atcgtcatgg
    20941 cctaaaattt ccataaagaa aacatcctca tctacgtttt tccaacgagg atctacgcgg
    21001 aaaaacattt catacgcaat acgggcataa atagcacgac tatctttata aacacggcgg
    21061 tcatctttga aaaatgattt gacccacggg ttaaaagcct ttgctaatat ggcattaatc
    21121 cgtccgtttt cagagcgtgt gtcgttatct ccataacctt taacgacatc atcaaaatca
    21181 gatgctgctg ggcatgagcg aagtacattt attaaataaa caaatgtttt tgcttcgcat
    21241 aatgtatata tttttctggt tatatcatca gaagttcttt tttttgcttg gcctaaaaaa
    21301 tcaacggtat acttaccaga aacagtaaat gtaccttgat acataatctc aatcattcgt
    21361 cttcctgaca atgcagcaag tgcaaatgcc aaaggagcca tacctgaacg agtgtttaga
    21421 ttatttaatg tagcagggct atttataata tcgtaaatag cttgcatata tttagggtaa
    21481 tcaatgacca ccacgttacg tttcttattg tgcagaacgt ccgcccatcg ttgctggata
    21541 gatgcgcgtt cggcagggct tagttgaaga tgatagagaa tctcatggtt aacttttagc
    21601 tgattcaact cttcaagtaa ggcagatcct tgctgtagta atttatataa ataatcgcgc
    21661 ttatctttcc agttatcgct agttaaatca gatatggcga attgccactc tttatattta
    21721 tttgataatt ttaataactt attatgactt ttgttatcac caattctgat attagataaa
    21781 tcttctgata aagggagaat taattttaat tttgcctgta aagctgacat tctctggcga
    21841 atatcggcag caggtagaga aagccatgac aatagctcct cattatataa aggatacttt
    21901 tcagataggc gatgtatatt tttttcaaag ctatgatgaa gtttatcatc aaaccgtttt
    21961 ctggccctgc tcatataggc attgaatgtg ttggctgtaa ttcttttttc taatcctttc
    22021 cccctaaact ttcgcttatc attaaataat gcatttttaa attttaatgc aacagccttt
    22081 attttttttg ttttttcccc ttgaggacgg tcagagttat ctattgactc aacatcgcta
    22141 acaagtgagt ttattaattc accgattttt actttgctca tctttcctct cctgattatc
    22201 agctctgatt atcgtaacac aatttgagaa taagataact aataacgcgt taacttagcc
    22261 acaaactaac agaacataat gacggataat aaacaaaaac acaatatcgg aacgttaaaa
    22321 tttgttcctt gactttgttc taaaaatcag cacataat
//
LOCUS       DAZPLM010000055        22286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPLM010000055 DAZPLM010000000
VERSION     DAZPLM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.055"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(85..3234)
                     /gene="acrB"
                     /locus_tag="ACHVC7_001746"
     CDS             complement(85..3234)
                     /gene="acrB"
                     /locus_tag="ACHVC7_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR2115826.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3257..4450)
                     /gene="acrA"
                     /locus_tag="ACHVC7_001747"
     CDS             complement(3257..4450)
                     /gene="acrA"
                     /locus_tag="ACHVC7_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR2115827.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4592..5239
                     /gene="acrR"
                     /locus_tag="ACHVC7_001748"
     CDS             4592..5239
                     /gene="acrR"
                     /locus_tag="ACHVC7_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR2115828.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5367..8729
                     /gene="mscK"
                     /locus_tag="ACHVC7_001749"
     CDS             5367..8729
                     /gene="mscK"
                     /locus_tag="ACHVC7_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR2115829.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8790..8888
                     /locus_tag="ACHVC7_001750"
                     /pseudo
     CDS             8790..8888
                     /locus_tag="ACHVC7_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8941..9102)
                     /gene="rsmS"
                     /locus_tag="ACHVC7_001751"
     CDS             complement(8941..9102)
                     /gene="rsmS"
                     /locus_tag="ACHVC7_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR2115830.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9116..9643)
                     /gene="priC"
                     /locus_tag="ACHVC7_001752"
     CDS             complement(9116..9643)
                     /gene="priC"
                     /locus_tag="ACHVC7_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR2115831.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9713..10090
                     /locus_tag="ACHVC7_001753"
     CDS             9713..10090
                     /locus_tag="ACHVC7_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR2115832.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10243..10794
                     /gene="apt"
                     /locus_tag="ACHVC7_001754"
     CDS             10243..10794
                     /gene="apt"
                     /locus_tag="ACHVC7_001754"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR2115833.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10923..12848
                     /gene="dnaX"
                     /locus_tag="ACHVC7_001755"
     CDS             10923..12848
                     /gene="dnaX"
                     /locus_tag="ACHVC7_001755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR2115834.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMPAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12181..12245
                     /gene="dnaX"
                     /locus_tag="ACHVC7_001755"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12901..13230
                     /locus_tag="ACHVC7_001756"
     CDS             12901..13230
                     /locus_tag="ACHVC7_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR2115835.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13230..13835
                     /gene="recR"
                     /locus_tag="ACHVC7_001757"
     CDS             13230..13835
                     /gene="recR"
                     /locus_tag="ACHVC7_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR2115836.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13945..15819
                     /gene="htpG"
                     /locus_tag="ACHVC7_001758"
     CDS             13945..15819
                     /gene="htpG"
                     /locus_tag="ACHVC7_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR2115837.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16000..16644
                     /gene="adk"
                     /locus_tag="ACHVC7_001759"
     CDS             16000..16644
                     /gene="adk"
                     /locus_tag="ACHVC7_001759"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR2115838.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16776..17738
                     /gene="hemH"
                     /locus_tag="ACHVC7_001760"
     CDS             16776..17738
                     /gene="hemH"
                     /locus_tag="ACHVC7_001760"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR2115839.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17735..18694)
                     /gene="aes"
                     /locus_tag="ACHVC7_001761"
     CDS             complement(17735..18694)
                     /gene="aes"
                     /locus_tag="ACHVC7_001761"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR2115840.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18846..20150
                     /gene="gsk"
                     /locus_tag="ACHVC7_001762"
     CDS             18846..20150
                     /gene="gsk"
                     /locus_tag="ACHVC7_001762"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR2115841.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20283..21959)
                     /gene="ybaL"
                     /locus_tag="ACHVC7_001763"
     CDS             complement(20283..21959)
                     /gene="ybaL"
                     /locus_tag="ACHVC7_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR2115842.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22197..>22286)
                     /locus_tag="ACHVC7_001764"
     CDS             complement(22197..>22286)
                     /locus_tag="ACHVC7_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115843.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5429 a         5915 c         5845 g         5097 t
ORIGIN      
        1 agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag gccgcttgcg
       61 cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc
      121 ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat
      181 tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc
      241 gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga
      301 ggtcatcagg atcggacgta aacgcatccg cactgcatca agcgtcgctt caatcagacc
      361 tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc
      421 cgacaaccca atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg
      481 gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa
      541 cggaatcgac cagctctcgt acagcgccgc cagacacagg aatacgacaa tcaacgaaat
      601 cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca
      661 gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc
      721 accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca ggccgttgta
      781 acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg agaatggcac
      841 catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta
      901 tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac
      961 atagctaccg ccccatgcag cgcccagaga ggtgttaatg tcgttgatag aaacacccag
     1021 cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt
     1081 tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca gctggttacg
     1141 cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc
     1201 ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt
     1261 gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc
     1321 cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct gaccacgtcc
     1381 cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag
     1441 atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg
     1501 cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg agcttggcag
     1561 acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca ggtaacgccc
     1621 cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat
     1681 gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg
     1741 tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta ccgacagcgc
     1801 cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa
     1861 gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg
     1921 aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac
     1981 acgctccacg ttttctacca caacgatagc gtcatccacc aacaagccga tggcgagcac
     2041 catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt
     2101 cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag
     2161 gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt taaccacttc
     2221 gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacccga
     2281 cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc
     2341 gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc
     2401 gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca gcagtacgcg
     2461 ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg
     2521 cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag
     2581 ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt
     2641 tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt actgtgaacc
     2701 gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca tattcgccgc
     2761 tacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac
     2821 catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa cttcttgcgg
     2881 cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc
     2941 agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga
     3001 catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc
     3061 ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg ccggcggtgc
     3121 aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat
     3181 gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta
     3241 acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct
     3301 ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca
     3361 gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc
     3421 caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg ccaactacca
     3481 gtacggtggc atcaccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt
     3541 ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc agagtgtgat
     3601 ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca acggtaacgt
     3661 cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca
     3721 ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca
     3781 ggaagtcgtt gctggactgg gtcacatcaa ggtagatcgg atcaagttgc tgcacggttg
     3841 ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gacttaccaa
     3901 tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc gcagtttcaa
     3961 cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc agagcctgat
     4021 cgtactcttg cttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca
     4081 attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca ctgtcgtatg
     4141 tcgcctgata ggtcgcagga tcaatctgat agagagagac accagcttcg atgtcgctac
     4201 cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc
     4261 ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg
     4321 ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc
     4381 ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt
     4441 ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa
     4501 taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat
     4561 gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca
     4621 agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc
     4681 atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg
     4741 gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat
     4801 tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag
     4861 agagatatta attcatgttc ttgaatccac ggtgacagaa gaacgacgtc gattattgat
     4921 ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca
     4981 acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga
     5041 agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta
     5101 tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga
     5161 agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa
     5221 tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga cattttcgtc
     5281 gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc
     5341 ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc
     5401 attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt
     5461 ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg
     5521 actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga
     5581 cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca gttcagctac
     5641 ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac
     5701 ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc
     5761 agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg
     5821 cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga
     5881 tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg
     5941 gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag gcgttgctga
     6001 atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg caggatacct
     6061 tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcac cagttgcagc
     6121 tgttgcaaga agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag
     6181 ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa caggaactgg
     6241 aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga
     6301 tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata
     6361 ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc
     6421 aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt
     6481 tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag agcgatgcgt
     6541 tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt
     6601 tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa cagttgggta
     6661 accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg agcgtatcga
     6721 aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac cgtccaatgg
     6781 actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa
     6841 tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gccggtttgc
     6901 cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac
     6961 agaaactggc ttccgctgtg ggttccctgc gtaacgatag ccagctcaac acaccaaaag
     7021 cgatccttat cgacctgatc cgcgcgctgc cggtgtgcct gattattctc gcggttggcc
     7081 tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc agtaaaaaac
     7141 tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa aatggcgttg
     7201 ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg caaattgtcc
     7261 gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa ctttccccgc
     7321 tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg ctgctgattg
     7381 ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc
     7441 gactggtcac cattaccgtg ctgtcgatta tcccgattgc attgatggtg ctgactgcca
     7501 cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg
     7561 tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta gcggcgcggc
     7621 gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag
     7681 gtgccgaacc gccggaagaa cccaccattg cactggaaca ggttaaccag cagacgctgc
     7741 gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg
     7801 atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac aacggcactg
     7861 aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg
     7921 cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa gtgctggtgc
     7981 tctcgcgact gaatatgcgt cagggcgcgt cgtatgccat tactaccatc cttaactaca
     8041 tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctct tgggataaac
     8101 tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa gaaattttcg
     8161 gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg
     8221 taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta
     8281 ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga
     8341 tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc gtggcctatg
     8401 gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg
     8461 tgatgcacga accaatgccg gaagtcttct ttacggcgtt tggtgccagc acgttggatc
     8521 atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag tcgtactgtc gatgagctga
     8581 accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg
     8641 aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtcaaacgc gactacaaag
     8701 gcgatgaccc gacgccagcg gtagggtaat aaccaaaggg ggcgacattt agttgccccg
     8761 agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc
     8821 tacgaaaccg caagaattca atgtattgca ggagcggtgt aggcctgata agcgtagcgc
     8881 atcaggcagt tttgcgtttg cccacaatct taggggacat ttagcggccc catttatttc
     8941 tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc
     9001 cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagcagca caatcaaatc
     9061 gacagccagt ttgacatcgt caggggcatt ttcaagtgac atactctctc cattgctagc
     9121 gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag cgtgccaggc
     9181 gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc
     9241 gcgccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca aaatcctgat
     9301 gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc cactcgcgca
     9361 atgaccaggc ggaggcttca cgcgcgatgg cttccagttg tgccgccaga tgttccgcca
     9421 gccaggccac ttgcggcagc tgttgctgct caaccgcatg acaaagcgca gccagattat
     9481 cgcccgcctc gtcgagacaa gcctgtagtg ttgtcgcacg agtctgaaaa agatgcctgt
     9541 cgaaacgagc acttagcgtg gcgaactgtg acaccggggc acaacgctga cgcagcgtag
     9601 cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga cctcaatgaa
     9661 aatgataatt gttatgctaa agtagccact ctgtaagctg acaacactca atatgcaacg
     9721 aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt
     9781 accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg cccgttcttc
     9841 cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc tacgtttctg
     9901 gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct
     9961 cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt
    10021 aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg aaaagcaaga
    10081 aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt acattctggc
    10141 gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt gatacgttta
    10201 aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc gactgcacag
    10261 cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa acccggcatt
    10321 cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct cagcatcgac
    10381 ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt
    10441 ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt accggtccgt
    10501 aaaccgggca aactgccgcg tgaaaccatc agtgaaactt acgacctgga atacggcacc
    10561 gatcagctgg agatccacgt tgatgccatc aaaccgggcg acaaagttct ggtggtggac
    10621 gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg tcgtctgggt
    10681 ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg cgaacagcgt
    10741 ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca ttaattatcg
    10801 ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc tcgccgttct
    10861 gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt cagagcctgc
    10921 caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg
    10981 gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt attcatcatg
    11041 cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga
    11101 aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc
    11161 gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca
    11221 aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg cgtggtcgtt
    11281 tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt
    11341 taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg accgatccac
    11401 agaaattgcc ggtgacgatt ttgtcgcgct gtttgcaatt tcatctcaag gcgctggatg
    11461 tcgagcaaat tcgccatcaa cttgagcaca tcctcaacga agaacatatc gctcacgagc
    11521 cgcgggcgtt gcaattgctg gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc
    11581 tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggca gtcagtgcga
    11641 tgctgggtac gcttgatgac gatcaggcgc tgtcgctggt tgaagcgatg gtcgaggcca
    11701 acggcgagcg cgtaatggcg ctgattaatg aagccgctgc ccgtggtatc gagtgggaag
    11761 cgttgctggt ggaaatgctc ggcctgttgc atcgtattgc gatggtacaa ctttcgcctg
    11821 ctgcacttgg caacgacatg cccgccattg agctgcggat gcgtgaactg gcgcgcacca
    11881 taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc aaagaattac
    11941 cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc
    12001 atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca cccgtcgtgc
    12061 caacggcagt aatgacgcca acccaggtgc cgccgcaatc agcgccgcag caggcaccga
    12121 ctgtaccgct cccggaaacc accagccagg tgctggcggc gcgccagcag ttgcagtgcg
    12181 tgcagggagc aaccaaagca aaaaagagtg aatcggcagc cgctacccgc gcgcggccgg
    12241 tgaataacgc tgcgctggaa agactggctt cggtcaccga tcgcgttcag gcgcgtccgg
    12301 tgccatcggc gctggaaaaa gcgtcagcca aaaaagaagc gtatcgctgg aaggcgacca
    12361 ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg
    12421 aacatgaaaa aacgccggaa ctggcggcga agctagcggc agaagccatt gagcgcgacc
    12481 cgtgggcggc acaggtgagc caactttcgc taccaaaact ggtcgaacag gtggcgttaa
    12541 atgcctggaa agaggagagc gacaacgcag tatgtctgca tttgcgctcc tctcagcggc
    12601 atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt
    12661 caacggttga actgactatc gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt
    12721 ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt attgcggata
    12781 ataatattca gaccctgcgt cggtttttcg atgcggagct ggatgaagaa agtatccgcc
    12841 ccatttgatc gtaagcacag cttacgttcg tcatccttaa cgtgattgag agagaaacct
    12901 atgtttggta aaggcggtct gggtaacctg atgaagcaag cccagcagat gcaagaaaaa
    12961 atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtca ccggcgaatc tggcgcaggt
    13021 ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc gcgtagagat cgacccgagc
    13081 ctgctggaag acgacaaaga gatgctggaa gacctggtgg ctgcagcatt caacgacgca
    13141 gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag
    13201 ctgccgcctg gctttaagat gccgttctga tgcaaaccag cccgctgtta acacagctta
    13261 tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca
    13321 cgctgcttca gcgcgatcgt agcggcggga tgcgtttggc gcaggcgctc acccgggcga
    13381 tgtcggaaat cggccactgc gccgattgcc gcactttcac cgaacaggaa gtctgtaaca
    13441 tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag agtccggcgg
    13501 acatctacgc cattgagcag acggggcagt tttctggtcg ttattttgtg ttgatggggc
    13561 atctgtcacc gctggacggc atcggtccgg atgatatcgg gcttgatcgt ctggaacagc
    13621 gtctggcaga ggaaaaaatc actgaagtga tcctcgccac caatcccacg gttgaaggtg
    13681 aagctaccgc taactacatt gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa
    13741 tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc acgctgtcac
    13801 actcccttgc cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg atcacctgct
    13861 ctcgcttgaa attattctcc cttgtcccca tctctccctc atcctgtttt taaccttaaa
    13921 atggcattat tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa
    13981 gtgaaacagc ttctgcacct gatgatccat tctctctatt ccaataaaga aatcttcctg
    14041 cgtgagctta tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct
    14101 aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac
    14161 aagcgcacgc tgaccatctc tgataacggc gtggggatga cccgcgacga agtgattgac
    14221 catctgggga ctatcgctaa atccggtacc aaatcattcc tcgaatccct gggttctgac
    14281 caggcgaaag acagccagct gatcggtcag tttggtgttg gtttctactc tgcgtttatc
    14341 gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc
    14401 ttctgggaat cggctggcga aggtgaatat accgttgccg acatcaccaa agaagatcgt
    14461 ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc
    14521 gtgcgttcca tcatcagcaa atactccgac catatcgcgc tgccggtaga gatcgaaaaa
    14581 cgcgaagaga aagacggcga aaccgttatc tcctgggaga aaatcaacaa agcgcaggcg
    14641 ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt acaaagagtt ctacaaacac
    14701 atcgcccacg actttaatga tccgctgacc tggagccaca accgcgttga aggtaagcag
    14761 gagtacacca gcctgctgta cattccgtcc caggctccgt gggatatgtg gaaccgtgat
    14821 cataaacacg gcctgaaact gtacgtccag cgtgtgttca tcatggacga cgcagaacag
    14881 ttcatgccga actatctgcg cttcgtgcgt ggtctgattg actccagcga tctgccgctg
    14941 aacgtttccc gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg caatgcgctg
    15001 accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga aaaataccag
    15061 accttctggc aacagtttgg cctggtactg aaagaaggtc cggcggaaga tttcgctaac
    15121 caggaagcga tcgccaaact gctgcgtttt gcttctaccc ataccgattc ttctgcgcag
    15181 accgtatctc tggaagacta cgtttcccgc atgaaagaag ggcaggagaa aatctactac
    15241 atcaccgcag acagctatgc ggcagcgaag agcagcccgc acctggaact gctgcgtaag
    15301 aaaggcatcg aagttctgct gctttccgac cgcatcgatg agtggatgat gaactatctg
    15361 actgagttcg acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa
    15421 ctggctgacg aagttgatga gagcgcgaaa gaagcggaga aagcactgac tccgttcatc
    15481 gaccgtgtga aagccctgct cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg
    15541 accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa
    15601 ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat acatcttcga actgaacccg
    15661 gatcacgtac tggtgaaacg tgcggcagat actgaagatg aagcgaagtt cagcgagtgg
    15721 gtagaactgc tgctggatca ggcgctgctg gcagaacgcg gcacgctgga agatccgaac
    15781 ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat gtcatgccgg atgacctttg
    15841 tgtcatccgg catttttctt ttcatcatct gcactttccg caaattatct cgccattaac
    15901 cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct
    15961 ttttcaaaaa aatcgacaca ttttaagggg attttcgcaa tgcgtatcat tctgcttggc
    16021 gctccgggcg cggggaaagg gactcaggct cagttcatca tggagaaata tggtattccg
    16081 caaatctcca ctggcgatat gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa
    16141 caagcaaaag acattatgga tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg
    16201 gttaaagagc gcattgctca ggaagactgc cgtaatggtt tcctgttgga cggcttcccg
    16261 cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg
    16321 gaattcgacg taccggacga actgattgtt gatcgtatcg taggccgccg cgttcatgcg
    16381 ccgtctggtc gtgtttatca cgttaaattc aatccgccga aagtagaagg caaagacgac
    16441 gttaccggtg aagaactgac tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt
    16501 ctggttgaat accatcagat gactgcaccg ctgatcggct actacttcaa agaagcggaa
    16561 gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagt cggttgctga agttcgcgct
    16621 gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc
    16681 tcatccacct caggcaatac ctctcacagc aattagttct tcttcctcac ttttccgcta
    16741 caattatcaa caagttgaat cgataagagg cggtaatgcg tcagactaaa atcggtatcc
    16801 tgctggcaaa cctgggtacg cccgataccc ccacacctga agcggtaaaa cgctatctga
    16861 aacaattttt aagcgacaga cgcgtggttg atacctcacg gttgttatgg tggccattgc
    16921 tgcgcggcgt tattttgccg ctgcgctcgc cgcgtgtggc gaagctgtac gcctcggtct
    16981 ggatggaagg tggctcgccg ctgatggttt acagccgcca gcaacagcag gcgctggcac
    17041 aacgtttacc ggagacgccc gtagcgctgg gaatgagcta cggctcgcca tcactggaaa
    17101 gcgccgtcga tgaactcctg gcagagcatg tagatcatat tgtggtgctg ccgctttatc
    17161 cgcaattctc ctgttctacg gtcggtgcgg tatgggatga actggcacgc attctggcgc
    17221 gcaaacgtag cattccgggg atatcgttta ttcgtgatta cgccgataac cacgattaca
    17281 ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa ccggatctgc
    17341 tgctgctctc ttatcatggc attccccagc gttatgcaga tgaaggcgat gattacccgc
    17401 aacgttgccg cacaacgact cgcgaactgg cttccgcact ggggatggca ccggaaaaag
    17461 tgatgatgac ctttcagtcg cgctttggcc gggaaccctg gctgatgcct tataccgacg
    17521 aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat acaggtgatg tgcccgggct
    17581 ttgctgcgga ttgtctggag acgctggaag agattgccga gcaaaaccgt gaggtcttcc
    17641 tcggtgcggg cgggaaaaaa tatgaatata ttccagcgct taatgccacg ccggaacata
    17701 tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag ctgagcggta aagaactgag
    17761 cgccgtcgcg aagagcctcg tcggcggttt tcatcatccg tgaataatgc aaaaaggcat
    17821 gcagcgtacc tgggtagagt ttgaactcac agggctgctg atgcgccgct aacgtctggt
    17881 aaagcagacg gctgtcatcc agcagcggat cgaactccgc cccggcaata aaacagggcg
    17941 gaacttcgcg agtgagatca ttattaaaca gacagtaata cggtgactcg cggtccgcgt
    18001 cgttgcttaa atacgcctct tcatacatct gcaaatcctg ttgcgttaag ccatcccaga
    18061 caccgcccaa cagacgacga gtcacggaat cccgtaatcc gtaaagccca taccacagca
    18121 aaacacccgc aattttaccg caatcgatct gtttatcacg caaccacaac gcactggcga
    18181 gcgccagcat ggcacctgcg gaatcaccgg caaagccaat gcgggacata ttgatttgat
    18241 aatcctccgc ctgctggtgg aaataacaac aagcagccac aatttcctct atcgcttgcg
    18301 gaaaacgcgc ttcaggtgaa agggtgtaat caataccaat caccgtacat tggctgtagc
    18361 ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt gccgagaata aaaccgcctc
    18421 catgcaaata aaatagcgtc gccgggctat ctggctgcgg acaaaagaga cgtgtttcca
    18481 cctgcccata ttttgttgga accatgtaag ctctggttgc catttctgga gcgcccgcat
    18541 tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg
    18601 gccaggacgg taaatccggc tgaagagtat tcacaacggt cttcatttca gcagaaataa
    18661 ggtccagaac aggtagtttg ttttccggct tcatacaaaa actcctttca aattacgtca
    18721 ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc tgatattgaa actggctgcg
    18781 tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct cagttaacca cccgtaaaaa
    18841 caaccatgaa atttcccggt aaacgtaaat ccaaacatta cttccctgta aatgcacgcg
    18901 atccgctgct tcagcaattc cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta
    18961 ttgaccagac gctggtcgat attgaagcga aagtggatga tgaattcatt gagcgttatg
    19021 gattaagcgc cgggcattca ctggtgattg aggatgacgt agccgaagcg ctttatcagg
    19081 agctaaaaca gaaaaacctg attacccatc agtttgcggg tggcaccatt ggtaacacca
    19141 tgcacaacta ctcggtgctc gcggacgacc gttcggtgct gctgggcgtc atgtgcagca
    19201 atattgaaat tggcagttat gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc
    19261 ttaactatct acaaggcgtg gatggcccga ttggtcgttg ctttacgctg attggcgagt
    19321 ccggggaacg tacctttgct atcagtccag gccacatgaa ccagctgcgg gctgaaagca
    19381 ttccggaaga tgtgattgcc ggagcctcgg cactggttct cacctcttat ctggtgcgtt
    19441 gcaaaccggg tgaacccatg ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat
    19501 ataacgtacc ggtggtgctg acgctgggca ccaagtttgt cattgccgag aatccgcagt
    19561 ggtggcagca attcctcaaa gatcacgtct ctatccttgc gatgaacgaa gatgaagccg
    19621 aagcgttgac cggagaaagc gatccgttgt tggcatctga caaggcgctg gactgggtag
    19681 atctggtgct gtgcaccgcc gggccaatcg gcttgtatat ggcgggcttt accgaagacg
    19741 aagcgaaacg taaaacccag catccgctgc tgccgggcgc tatagcggaa ttcaaccagt
    19801 atgagtttag ccgcgccatg cgccacaagg attgccagaa tccgctgcgt gtctattcgc
    19861 acattgcgcc gtacatgggc gggccggaaa aaatcatgaa cactaatgga gcgggggatg
    19921 gcgcattggc agcgttgctg catgacatta ccgccaacag ctaccatcgt agcaacgtac
    19981 caaactccag caaacataaa ttcacctggt taacttattc atcgttagcg caggtgtgta
    20041 aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg
    20101 gcttgccgga gcgtgaagac agcctggaag agtcttactg ggatcgttaa gttatcgtcg
    20161 gttcgtaggc cagataaggc gttcacgccg catctggcat ttggctctcg atgcctgatg
    20221 cgacgctggc gcgtcttatc atgcctacat atttttcata ttttacatcc ggcaaccacc
    20281 gtttaccccg tcaccacttc acccgccggt ggcgtttcca gcagttccag catagtacgg
    20341 gcaatttcac gttcacccat cactacctga ttcgcgccac gttcggtgat atacgtcact
    20401 tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg ggtttttcgc gcgggcagac
    20461 gccacaatct cacccgcttc ataaccgttg gggatcgtca ggatcagcca ttttgcacat
    20521 tccagatgcg ccagttgcat aatttcttcg ttcgctgcat tgcccaatac agcgcggacc
    20581 ccacgctctc gcagctcatc aacacgggtt cgtgacgtct caatcaccac cagcggaata
    20641 tcagaggcga gcaatttctc ccccagcagg ctgcctacac gaccgtaacc caccaatagc
    20701 gcatggttgc aaatatccac tgggatctgt ttctcttctt cgattgcctc ttccagcgtc
    20761 tgctcttcca gcgtttcggt cttcgccaga tatttctcca gtagtgcgaa cagtaccggg
    20821 ttgagcataa tcgacaggat cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt
    20881 aaattcaatg ccattcccag tcccgccagg ataaacgcga actcaccaat ctgcgccagg
    20941 ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa acagacggac aaggaacagg
    21001 gccgcgaccg atttaccaaa cagaataatt gccagcgtcg ccagcactgc cagcggttgc
    21061 tgaatcagaa ttaacggatc aaacaacatc ccgacggaga caaaaaacag caccgcaaac
    21121 gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac tcagttcaga ctcgttcagt
    21181 accatcccgg caaagaacgc gccaagtgcg aaggagacgt caaacaactc caccgcacca
    21241 aaggcaatcc ctaacgccag cgccagcacc gacagggtaa atagctcgcg agaaccggtt
    21301 gccgcgctgc gtgccataat ccacggcacc agacggcgac ctaccagcat cataatggcg
    21361 ataaatgcga tcactttgcc gatggtgatc cccatatcga ctgcaagagt ggcaaagccc
    21421 acatcgccct gttccatcat tcctgccact gcgggcagca acaccagcgt cagaaccatt
    21481 accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact gtcaattaat
    21541 tgccgttctt caagtgcgcg cagtaacacc acggtactgg cggtggaaag acataaaccg
    21601 aacacgatac cggtcattaa cgaccagccc agcacggcag agagcgccat acccagcagc
    21661 gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga tggcctttac cgccatcaaa
    21721 tccttcagcg aaaagtgcaa accgacgcca aacatcaaca gaatgacgcc cagttcagcc
    21781 agttccggcg caagcttggt atcggcaaca aagcccggag tgaatggtcc tgccagcaca
    21841 cccgctaaca gatatcccac cagaggagaa atacgtagtt tattggccag catgccgagg
    21901 ataaaggcga gcacaaggcc gccaacaatg gtggtgataa gcggggtggc gtgatgcatt
    21961 ccgtctcctt ttcctggtgg ttattgtcca tttttggccg ggaaaaccaa aattacaggt
    22021 aatagtttat gacaatttca ttgatgatgt tcatgaataa ttattgaatt ttgcagaaaa
    22081 atggaattag ctgcaaaaaa agcacggatc ggaaaacaga aggcggttta acagaggaaa
    22141 ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca atgaaatcag
    22201 tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg caggaaagca
    22261 cagattttat agactaactc gatgct
//
LOCUS       DAZPLM010000056        21947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPLM010000056 DAZPLM010000000
VERSION     DAZPLM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.056"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHVC7_001765"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHVC7_001765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR2115844.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHVC7_001766"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHVC7_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR2115845.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHVC7_001767"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHVC7_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR2115846.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHVC7_001768"
     CDS             2284..2730
                     /locus_tag="ACHVC7_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR2115847.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHVC7_001769"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHVC7_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR2115848.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHVC7_001770"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHVC7_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR2115849.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHVC7_001771"
     CDS             4841..5188
                     /locus_tag="ACHVC7_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR2115850.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHVC7_001772"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHVC7_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR2115851.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHVC7_001773"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHVC7_001773"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR2115852.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="ACHVC7_001774"
     CDS             6705..6803
                     /locus_tag="ACHVC7_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR2115853.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHVC7_001775"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHVC7_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR2115854.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="ACHVC7_001776"
     CDS             complement(6939..7187)
                     /locus_tag="ACHVC7_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR2115855.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="ACHVC7_001777"
     CDS             complement(7335..7517)
                     /locus_tag="ACHVC7_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR2115856.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHVC7_001778"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHVC7_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR2115857.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHVC7_001779"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHVC7_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR2115858.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHVC7_001780"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHVC7_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR2115859.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHVC7_001781"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHVC7_001781"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR2115860.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHVC7_001782"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHVC7_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR2115861.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHVC7_001783"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHVC7_001783"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR2115862.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHVC7_001784"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHVC7_001784"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR2115863.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHVC7_001785"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHVC7_001785"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR2115864.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHVC7_001786"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHVC7_001786"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR2115865.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHVC7_001787"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHVC7_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR2115866.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHVC7_001788"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHVC7_001788"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR2115867.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21394)
                     /locus_tag="ACHVC7_001789"
                     /pseudo
     CDS             complement(19482..21394)
                     /locus_tag="ACHVC7_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            21526..21870
                     /locus_tag="ACHVC7_001790"
     CDS             21526..21870
                     /locus_tag="ACHVC7_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR2115868.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5364 a         5641 c         5361 g         5581 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcacaaggaa acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg
    19561 cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatt accagcacgc
    19621 cgcgatcgtc ggcgtcgcga atcagtcgcc ctactccctg cttgagagta atgaccgcat
    19681 ccggcagctg cacttcatcg aacgggtcgc caccgcgcaa acgacaatct tccatgcgcg
    19741 cttttaacag tagatcatcc ggcgaggtaa acggcaattt gtcgataatt accaatgaca
    19801 atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt
    19861 taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    19921 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcatgcgagg
    19981 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcca
    20041 gttgccgtgc acaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    20101 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    20161 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    20221 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    20281 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    20341 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    20401 gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    20461 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    20521 gataacctgg ctcaccgagt tgcagacgaa aatcctgtgc actctgggca agacgatcag
    20581 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    20641 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    20701 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttcagggatc aactcgccaa
    20761 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    20821 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    20881 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg
    20941 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    21001 ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    21061 gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    21121 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    21181 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    21241 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    21301 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    21361 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    21421 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg
    21481 gcagtctttg cagcacgaaa atggaagtat aatgaggaca aaatgatgac tatcgttcgt
    21541 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    21601 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcacag
    21661 attgacgccg tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt
    21721 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc
    21781 gcgggtcatg cgccagtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    21841 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    21901 aatttatcga taatactggt cggttttaca taaatcgacc agagaat
//
LOCUS       DAZPLM010000057        21643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPLM010000057 DAZPLM010000000
VERSION     DAZPLM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.057"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHVC7_001791"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHVC7_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR2115869.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHVC7_001792"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHVC7_001792"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR2115870.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHVC7_001793"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHVC7_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR2115871.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHVC7_001794"
     CDS             complement(3717..4511)
                     /locus_tag="ACHVC7_001794"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR2115872.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHVC7_001795"
     CDS             4649..4990
                     /locus_tag="ACHVC7_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR2115873.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHVC7_001796"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHVC7_001796"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR2115874.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHVC7_001797"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHVC7_001797"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR2115875.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHVC7_001798"
     CDS             8906..10198
                     /locus_tag="ACHVC7_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR2115876.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHVC7_001799"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHVC7_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHVC7_001800"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHVC7_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR2115877.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHVC7_001801"
     CDS             complement(10731..11189)
                     /locus_tag="ACHVC7_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR2115878.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHVC7_001802"
     CDS             complement(11186..12103)
                     /locus_tag="ACHVC7_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR2115879.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHVC7_001803"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHVC7_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR2115880.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHVC7_001804"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHVC7_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR2115881.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHVC7_001805"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHVC7_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR2115882.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHVC7_001806"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHVC7_001806"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR2115883.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHVC7_001807"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHVC7_001807"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR2115884.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHVC7_001808"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHVC7_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR2115885.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHVC7_001809"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHVC7_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR2115886.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHVC7_001810"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHVC7_001810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR2115887.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHVC7_001811"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHVC7_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5685 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgttg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctaggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZPLM010000058        21455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPLM010000058 DAZPLM010000000
VERSION     DAZPLM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.058"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHVC7_001812"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHVC7_001812"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR2115888.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHVC7_001813"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHVC7_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR2115889.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="ACHVC7_001814"
     CDS             complement(2378..2992)
                     /locus_tag="ACHVC7_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR2115890.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLMTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHVC7_001815"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHVC7_001815"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR2115891.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="ACHVC7_001816"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="ACHVC7_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR2115892.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="ACHVC7_001817"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="ACHVC7_001817"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR2115893.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHVC7_001818"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHVC7_001818"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR2115894.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHVC7_001819"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHVC7_001819"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR2115895.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHVC7_001820"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHVC7_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR2115896.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHVC7_001821"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHVC7_001821"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR2115897.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHVC7_001822"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHVC7_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR2115898.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHVC7_001823"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHVC7_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR2115899.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHVC7_001824"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHVC7_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR2115900.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHVC7_001825"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHVC7_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR2115901.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHVC7_001826"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHVC7_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR2115902.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHVC7_001827"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHVC7_001827"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR2115903.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHVC7_001828"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHVC7_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR2115904.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHVC7_001829"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHVC7_001829"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR2115905.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="ACHVC7_001830"
     CDS             19125..19937
                     /locus_tag="ACHVC7_001830"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR2115906.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21014
                     /gene="tssD"
                     /locus_tag="ACHVC7_001831"
                     /pseudo
     CDS             20331..21014
                     /gene="tssD"
                     /locus_tag="ACHVC7_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            20994..>21455
                     /locus_tag="ACHVC7_001832"
     CDS             20994..>21455
                     /locus_tag="ACHVC7_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115907.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5266 a         5741 c         5373 g         5075 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcact ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcatta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagcggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcagcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcatgt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaagc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAZPLM010000059        21191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPLM010000059 DAZPLM010000000
VERSION     DAZPLM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.059"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            108..392
                     /gene="pduA"
                     /locus_tag="ACHVC7_001833"
     CDS             108..392
                     /gene="pduA"
                     /locus_tag="ACHVC7_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR2115908.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            389..1201
                     /gene="pduB"
                     /locus_tag="ACHVC7_001834"
                     /pseudo
     CDS             389..1201
                     /gene="pduB"
                     /locus_tag="ACHVC7_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1220..2884
                     /gene="pduC"
                     /locus_tag="ACHVC7_001835"
     CDS             1220..2884
                     /gene="pduC"
                     /locus_tag="ACHVC7_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR2115909.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2894..3562
                     /locus_tag="ACHVC7_001836"
     CDS             2894..3562
                     /locus_tag="ACHVC7_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR2115910.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3577..4095
                     /gene="pduE"
                     /locus_tag="ACHVC7_001837"
     CDS             3577..4095
                     /gene="pduE"
                     /locus_tag="ACHVC7_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR2115911.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4105..5937
                     /locus_tag="ACHVC7_001838"
     CDS             4105..5937
                     /locus_tag="ACHVC7_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR2115912.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5927..6277
                     /locus_tag="ACHVC7_001839"
     CDS             5927..6277
                     /locus_tag="ACHVC7_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR2115913.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6297..6572
                     /gene="pduJ"
                     /locus_tag="ACHVC7_001840"
     CDS             6297..6572
                     /gene="pduJ"
                     /locus_tag="ACHVC7_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR2115914.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6596..7018
                     /locus_tag="ACHVC7_001841"
     CDS             6596..7018
                     /locus_tag="ACHVC7_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR2115915.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7021..7653
                     /locus_tag="ACHVC7_001842"
     CDS             7021..7653
                     /locus_tag="ACHVC7_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR2115916.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7650..8141
                     /gene="pduM"
                     /locus_tag="ACHVC7_001843"
     CDS             7650..8141
                     /gene="pduM"
                     /locus_tag="ACHVC7_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR2115917.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8145..8420
                     /gene="pduN"
                     /locus_tag="ACHVC7_001844"
     CDS             8145..8420
                     /gene="pduN"
                     /locus_tag="ACHVC7_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR2115918.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8432..9439
                     /gene="pduO"
                     /locus_tag="ACHVC7_001845"
     CDS             8432..9439
                     /gene="pduO"
                     /locus_tag="ACHVC7_001845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR2115919.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9436..10821
                     /gene="pduP"
                     /locus_tag="ACHVC7_001846"
                     /pseudo
     CDS             9436..10821
                     /gene="pduP"
                     /locus_tag="ACHVC7_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10832..11944
                     /locus_tag="ACHVC7_001847"
     CDS             10832..11944
                     /locus_tag="ACHVC7_001847"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR2115920.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11941..13284
                     /locus_tag="ACHVC7_001848"
     CDS             11941..13284
                     /locus_tag="ACHVC7_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2115921.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13287..13841
                     /gene="pduT"
                     /locus_tag="ACHVC7_001849"
     CDS             13287..13841
                     /gene="pduT"
                     /locus_tag="ACHVC7_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR2115922.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13841..14191
                     /gene="pduU"
                     /locus_tag="ACHVC7_001850"
     CDS             13841..14191
                     /gene="pduU"
                     /locus_tag="ACHVC7_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR2115923.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14196..14639
                     /locus_tag="ACHVC7_001851"
     CDS             14196..14639
                     /locus_tag="ACHVC7_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR2115924.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14740..15069)
                     /locus_tag="ACHVC7_001852"
     CDS             complement(14740..15069)
                     /locus_tag="ACHVC7_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR2115925.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15241..16299)
                     /locus_tag="ACHVC7_001853"
     CDS             complement(15241..16299)
                     /locus_tag="ACHVC7_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR2115926.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16497..16970)
                     /gene="sbmC"
                     /locus_tag="ACHVC7_001854"
     CDS             complement(16497..16970)
                     /gene="sbmC"
                     /locus_tag="ACHVC7_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR2115927.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17089..18255)
                     /gene="dacD"
                     /locus_tag="ACHVC7_001855"
     CDS             complement(17089..18255)
                     /gene="dacD"
                     /locus_tag="ACHVC7_001855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR2115928.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18464..19891
                     /gene="sbcB"
                     /locus_tag="ACHVC7_001856"
     CDS             18464..19891
                     /gene="sbcB"
                     /locus_tag="ACHVC7_001856"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR2115929.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19934..20161)
                     /gene="tsuB"
                     /locus_tag="ACHVC7_001857"
     CDS             complement(19934..20161)
                     /gene="tsuB"
                     /locus_tag="ACHVC7_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR2115930.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20175..>21191)
                     /gene="tsuA"
                     /locus_tag="ACHVC7_001858"
     CDS             complement(20175..>21191)
                     /gene="tsuA"
                     /locus_tag="ACHVC7_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR2115931.1"
                     /translation="LGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFA
                     LIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTY
                     MVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKK
                     PKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSP
                     TANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGV
                     LMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLAT
                     AAAN"
BASE COUNT         5443 a         5538 c         5264 g         4946 t
ORIGIN      
        1 aatatttgtt ctctattaat ttctctcatt agccgattaa gtcagccatt ctttttcctc
       61 gctttttttt atagtcatat cgtcaaaaaa atccaggcga ggtctttatg caacaagaag
      121 cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat agaggccgca gacgccatgg
      181 tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat aggttccggg ctggtgaccg
      241 tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac agacgcgggt gccgcagctg
      301 cgcataacgt cggcgaagta aaagccgtac acgttattcc ccgcccacat accgatgtcg
      361 aaaaaatctt accaaaggga attaacccat gagcagcaat gaactggtcg aacagatcat
      421 ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag gttctcccga aaaccactca
      481 tcaaatacga tagacggcta tggcagaaaa aagctgcacg ctaacagaat ttgttggcac
      541 cgctattggc gacactgtag gtctggtcat tgcgaacgtt gatagcgccc tgttggacgc
      601 gatgaaactt gaaaaacgct atcgttccat tggcattctg ggtgcgcgta ttggcgcagg
      661 gccacacatt atggccgctg atgaagcggt aaaagccacc aacactgaag ttattagcat
      721 tgaacttcct cgcgatacta aaggcggtgc cggacacggc tcactgatcc tcttaggtgg
      781 caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc ctgaaagaac tcgatcggac
      841 ctttggcgat gtctatgcca acgaagccgg acatcttgaa ctgcaatata ccgcccgcgc
      901 cagctatgca ctggaaaaag cgtttggtgc accactggga cgggcttgcg gcgttattgt
      961 cggcgctccg gctgctgtgg gtgttctgat ggccgacacc gcgctgaagt ccgccaatgt
     1021 ggatgttgtg gcatacagct ccccagctca aggcaccagc tacagcaatg aagtcattct
     1081 gattatctcg ggtgactccg gcgcagtccg ccaggcggtc atctccgcac gcgaaatcgg
     1141 taaaaccgtg ctttcgactc tcggcgatac accgaaaaac gatcaccctt cctacatctg
     1201 aattctctga ggcagattta tgagatcgaa aagatttgaa gcactggcaa aacgcccggt
     1261 caaccaggac ggtttcgtta aagagtggat tgaagaaggc tttattgcga tggaaagccc
     1321 gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc gttaccgaac tggatggaaa
     1381 acccgccagc cagtttgacc tgatcgacca ctttatcgcc cgctacggca tcaatctcgc
     1441 gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt gccaatatgt tgtgcgatcc
     1501 aaatgtcaaa cgtagcgata tcgttcccct taccacagca atgacgcctg caaaaatcgt
     1561 cgaagtggtt tcgcagatga acgtggtaga gatgatgatg gcgatgcaga aaatgcgcgc
     1621 ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc aaagataacc cagtacaaat
     1681 tgccgccgac gcagctgaag gcgcatggcg cgggtttgat gaacaggaaa ccaccgttgc
     1741 cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg gtcggctcac aggtgggacg
     1801 tccgggcgtc ttgacccagt gttcactgga agaagccacc gaactgaaac tcggtatgtt
     1861 aggccacacc tgttacgccg aaacaatttc tgtctatggt acagaacctg ttttcaccga
     1921 tggtgatgat accccgtggt caaaagggtt tctcgcgtca tcctatgctt ctcgtggctt
     1981 aaaaatgcgc tttacttccg gctccggctc tgaagtacag atgggttacg cggaaggcaa
     2041 atcaatgctt tacctcgagg ctcgttgcat ttacatcacc aaagccgcag gcgttcaggg
     2101 tctgcagaat ggttcggtaa gctgcatcgg tgttccatca gctgtgccat caggcatccg
     2161 cgcagtatta gcggaaaact taatttgctc atcgctggat ctggaatgcg cctccagtaa
     2221 tgaccagacc tttacccact cggatatgcg ccgtaccgca cgtttcctga tgcaatttct
     2281 gccaggcacc gactttatct cttccggttt ttccgctgtg ccgaactacg acaacatgtt
     2341 tgctggttca aacgaagatg ctgaagactt cgatgattac aacgtcattc agcgtgacct
     2401 gaaggttgat ggtgggctgc gaccagtacg cgaagaagat gttatcgcta tccgcaataa
     2461 agccgctcgc gctttacagg ctgtctttgc cggtatggga ctaccgccta ttaccgatga
     2521 agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat atgcctgagc gcaatattgt
     2581 ggaagacatc aagttcgccc aggagattat caataaaaac cggaacagcc tggaagtggt
     2641 gaaagccttg gcgcaaggcg gttttaccga tgtcgcccag gacatgctca acatgcaaaa
     2701 agcaaaactg accggcgatt atctccatac ctcagccatc atcgtcgatg acggacaagt
     2761 gctctctgcg gtcaatgacg tcaatgatta cgccggaccg gctacaggtt accgcctgca
     2821 aggggaacgc tgggaagaga tcaaaaatat tcccggtgca cttgatccca acgaaattga
     2881 ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt cagattatcg aagacgtgct
     2941 ggcagaaatg caacccagcg acaagtccgt ttcatttcgc gcacctgtct ctgcaactgt
     3001 cccttccgcg ccagataccg gaaattttct gaccgaaatt ggcgaagccc aacaaggcac
     3061 tcagcaagat gaagtcatta ttgctgtagg cccggcgttc ggcctggcgc aaaccgtcaa
     3121 tatcatcggt attccgcata aaaacatctt gcgcgaagtg attgccggta tcgaagaaga
     3181 aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc gatgtcgcat tcgtagcagt
     3241 cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt attcagtcga aaggcacaac
     3301 ggttatccac cagcagggac ttcctccctt gtctaacctg gaacttttcc cacaagcgcc
     3361 tttgttgacg ctggatactt atcgtcagat tggcaaaaac gccgcgcgtt atgcgaagcg
     3421 cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg gcgcggccga aataccaggc
     3481 caaatctgcc attttgcata ttaaagagac gaagtacgtg gtcactggca aaaaaccgca
     3541 agaactgcgc gtgacgtttt aacaaaggaa accttcatga ataccgatgc aattgagtcg
     3601 atggtcaggg acgtgttgaa ccgcatgaac agtttacagg atgcagctcc tgtttctgcg
     3661 gtccccaacg catcaatact gagcgccaaa gtgacggatt acccgctggc aaataaacat
     3721 ccggaatggg ttaaaacagc gaccaataaa acgctcgacg atttcactct ggaaaatgtg
     3781 ctaagcgata acgtcaccgc cctggatatg cgcattaccc ctgaaacatt gcgtattcag
     3841 gctgcgattg ccagagatgc tggtcgcgat cgtctggcga tgaattttga acgcgccgct
     3901 gagcttacct cagtaccaga tgatcgcatc ctcgaaatct acaatgcgtt acgcccttat
     3961 cgttcaacta agcaagaact tattgccatt gctgacgatc ttgaacagcg ttatcaggcc
     4021 aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt atgtcgagcg caaaaaactg
     4081 aaaggcgacg attaaggacg aactatgcga tatatagcag gcattgatat cggcaactca
     4141 tcaacagagg ttgcgctggc aacgctcagt gcaactggag agttgtcgtt cgtgagtagc
     4201 gccctggagg aaaccaccgg aatcaaaggt acgttgcgta acgtccatgg tattcaggag
     4261 gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta gcgatatttc gcttattcgt
     4321 atcaacgaag caactccggt cattggtgat gtggcgatgg aaaccatcac tgaaactatc
     4381 atcaccgaat caaccatgat cggtcacaac ccgaaaactc cgggcggagt ggggctgggc
     4441 gtagggttca ccatcactcc gcaagagtta ttaacacgcc cggcggacac accttacatc
     4501 ctggtggtgt catcggcatt tgattttgcc gatatcgcga cgatgataaa cgcctccgta
     4561 cgcgccggat atcagctgac tggcgttatt ctgcaacgcg acgatggcgt actggtcaat
     4621 aaccgcctgg aaattccttt acctattgtg gatgaagtcc tctacatcga tcgtattccg
     4681 ctcggcatgc tggcagcaat tgaagttgcc gttcccggga aagttatcga aaccctgtct
     4741 aacccgtatg ggattgccac cgtattcgcc ctcaatgctg aagagactaa aaatattgtt
     4801 cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg tagtgaaaac gccctctggc
     4861 gacgttaaag cccgttcgat acccgccgga aatatcgaac tgctttctgc cgggcgcaca
     4921 acgcgcgttg atgtagctgc tggtgccgat gccattatga aagcggtcgg tgagtgccca
     4981 aaactggaaa acgtgacggg agaaccaggc accaacatcg gcggaatgct ggaacatgtt
     5041 cgccagacca tggcggaact gaccaacaaa cccagcaacg aaatttttat tcaggatttg
     5101 ctggcgatag atacctctgt ccccgtcagc gtaaccggtg gtctggcagg tgaattttca
     5161 ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg accgtctgca aatggcgatg
     5221 atcgccagtg aaattaagca aaaactgcat gttgatgttc aggtcggagg cgcagaagca
     5281 gaagccgcca ttcagggggc cttgactacc cctggaacca cacgtccact ggcaattctg
     5341 gacttaggtg caggctctac cgatgcctcc atcatcaatc agagtgggga aatcgttgcc
     5401 actcaccttg cgggagcggg cgacatggta acgatgatta tcgctcgtga gctgggctta
     5461 aatgatcgtt atctggcaga agaaatcaaa aagtacccgt tagcaaaagt agaaagcctg
     5521 ttccacttac gccatgaaga cggtagcgtt cagttcttcc ctactcccct atccccccat
     5581 gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg tgcctattcc cggtgatttg
     5641 acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag agcgtgtctt cgtaaccaat
     5701 gccctgcgcg cgctacgtca ggtaagccct gccggaaata ttcgcgacat tccgttcgtc
     5761 gttctggtgg gtggttcttc gctggatttc gaagtaccgc aattggtcac cgacgcgctg
     5821 gcacattatc gactggtcgc cgggcggggg aatatccgtg gtagcgaagg tccgcgcaac
     5881 gcggtagcta ctgggctact catcgcctgg cacaaggagt cgattcatgg aaagtaaaga
     5941 gcgtgcacca gccatcgttg tcatggagat cggtgactgt atcactacat gggatgaggt
     6001 gcttcttggc attgaagagg aaggtattcc ctttcgcatt caacacattc cgtcaggtga
     6061 agttatcgat agcgcctggc tggctgctcg tcaatctccc ctgttggttg gcattgcctg
     6121 cgaccgggaa gaactgattg tgcattacaa aaacttaccg gcatcagcgc cactgtttac
     6181 gctgacgtat caacaagata accatacccg gcgcagcatc ggcactaacg ctgcccgcct
     6241 ggtaaaagga attcccttcc gggaattcca ctcataatcc acaggagaaa cgctgtatga
     6301 acaacgcact aggactggtt gaaacaaaag ggttagttgg cgcaattgaa gctgcagatg
     6361 ccatggttaa atccgccaac gtgcagctgg ttggctatga aaagattggt tcgggtctga
     6421 ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc cgccgttgat gcggggagcg
     6481 ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt catcccgcgc ccacacagtg
     6541 atgttgaggc tattttacct aaatcggcat aaccccattt gctaaggagc acagcgtgaa
     6601 gcaatcactg ggattacttg aagccagtgg tctggctctc gccatcagtt gcgcagacgc
     6661 tatggcaaaa gccgcgtcca tcacgctggt tggccttgag aagaccaatg gctcaggctg
     6721 gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat gcggcgatcg cgacgggagc
     6781 cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa gtgattgccc gcccgggaga
     6841 cggcattctg ccccacacaa ctgaagcatt gaccgtgacg gttgaaacaa gcaaaacacc
     6901 agcaacaacc gtcacctcag agattatcag ttgtaatttg tgtcttgacc cggcatgtca
     6961 gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc aataagcgag gggagtagca
     7021 gtggataaac aaaccttgca ggaaacagtg agccagattc tggatgagat gcgcgagcgc
     7081 cctatcccac tgggcgtttc cagtcgccat atacatcttt catcagcgga ttatttgcgt
     7141 ctttttcctg aacaaccgat tagcgagaaa aagccgttaa tgcaacctgg ccaatacgct
     7201 gctgaacagg ttgttacgct ggagggacca aaaggcaaat tgcacaacgt tcgcttgttg
     7261 gggccgctac gcaatgcaag ccaggtggaa atttcccgga cggacgccag aacgttaggg
     7321 atcaccgccc cgctacgcat gtcaggcgat ctcagcggca ctccggggat tcgtttgata
     7381 agcccatttg cggaactgga gatcacatct ggcgtcattg tcgcgcaacg gcatattcat
     7441 atgtctccgc tggatgccct gatttatggc gttacgcatg gcgataaagt ggcggttgcc
     7501 attgaaggtg atgagcgacg actgatattc gaaaacgtcg ctgttcgcgt ctctccggat
     7561 atgcgtcttg aaatgcatat tgataccgat gaggccaacg ccgcaggcgc tgatagccct
     7621 gacgtttttg ccacactggt gtcagtgcca tgaataacat gctgatgcaa cgtattgttg
     7681 acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac actcgtgctg actgtttttc
     7741 aattgcgaga cgccagcgtt caagagagcg ttcatcagta tgcgtcatta caaatccgat
     7801 atgtcgatct gccgctgctg cgacaacttg cggagaacga aacgtcagat cgagcatcta
     7861 tccagataca cgaagcgtta gcctggggaa tgcacattca attgtcgttg cagcgtcatt
     7921 tcctgaacgc catcgaattg aagactctcg cccgtctacc gctaagctgg tgcgacgaac
     7981 aagggcagcc gatctacctt catcataatc gacttttaag ttatgccgat atcgcacagt
     8041 taagctcggg aatactggtg ttacagcgga aatgttgtgt gaccgcactg gctcgtgaag
     8101 cggcaattac ccgaaatatt cagttaatca ggcaggagta acctatgcat ctggctcgag
     8161 tcactggcgc ggtagtatcc acgcaaaaat caccttctct gatcgggaaa aaactgctgc
     8221 tggttcgccg ggtcagtgct gacggcgaac tgcctctcac gccattaacc ggcgacgaag
     8281 ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt actcctcagc agtggttcca
     8341 gcgccagaca tgtgttttca ggccctaacg aggccatcga cctggcagtg gtcggcattg
     8401 tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt tatacccgaa caggtgattc
     8461 aggaagtacg tctttattta ccggacagcg cgtcagcaaa actcatctac gtgtcgaaac
     8521 ctatggcacg ctggatgaac tgaacgcgac actcagtctg tgttattgcg ccaccgccat
     8581 cgaaagccat cgcatcttac tggaagccat tcaacaacag atattttggt ttagcgccga
     8641 actcgccagt gaaagcgaac agccgtcagc gcaacagcgt tacatcggta cggaagaaat
     8701 tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc gtccccccag ttcacagctt
     8761 cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat ttcgcccgca cggtggctcg
     8821 acgggctgaa agacggctgg ttgaactgac aacggaaacc accgtccgga atgttttgct
     8881 gcactatatc aaccgacttt ccgattgcct gtatgccctc gcccgcgtgg aagacaacgt
     8941 tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt tatcacgaag ccaaccacac
     9001 accagcattg aaggaacgca caatgccgct cacttttcag gatcttcacc agctcattcg
     9061 tagtgcggca atgcgcgcgg atgaactgca tattcccgtt gtcatcagca ttgttgatgc
     9121 caacggaaca gaaagtgtta cctggcggat gcctgatgcc ctgttggtca gcagtgaact
     9181 ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg gcaacccaca agcttgctga
     9241 taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc catatgcaag gaaaagtcgt
     9301 cacttttggt ggcggcttcc ccctctggcg tgacggaaaa ttgcttggcg ggcttggcat
     9361 cagcggcggt agcgttgaac aagacatgga tattgcccaa agcgcaatgg cggcaattaa
     9421 cgtgggagta aaccaatgaa taactctgaa ttagaaacac tgatccgcaa tattctgcgt
     9481 gagcaacttg tccccgctac accacgaacg caaagaaacg ctattttcca gactgtcgat
     9541 gaagcggtct gcgcagcaca ctaggccttt ttacgctttc aacagtgccc gcttaaaacc
     9601 cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc cccgccttac cgaactggca
     9661 gaagaaagcg ccaaagaaac ggggatggga aataaagaag ataagtacct aaaaaacaaa
     9721 gcggcactcg ataacacgcc aggaattgaa gatttaacca ccacagcact aaccggtgat
     9781 ggtggcatgg ttttgtttga gtactctccg tttggggtga ttggctcggt cacgccaagc
     9841 accaacccca cggaaaccat cattaacaac agcatcagca tgctggcagc aggtaatagc
     9901 gtctacttca gtccacaccc gggtgccaaa aacgtttcgt taaaactgat taccacaatc
     9961 gaagacatcg cgtttcgctg tagtggcatt cataacttga tcgttaccgt atctgaaccg
    10021 acctttgctg ccaccgaaca gatgatggcg caccctaaaa tcagcgttct ggcgattacc
    10081 ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca agaaagtgat tggtgccgga
    10141 gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata ttgtgaaagc cgccgaggac
    10201 atcatcaacg gcgcttcatt tgattacaac ctgccgtgta ttgctgaaaa aagcctgatt
    10261 gtcgtggcat cagtggcaga ttatttaatt cagcagatgc aaagttttgg cgcacttctt
    10321 cttaactatg agcaaactga gaaattgcgt gcaatttgcc tgccagacgg tagcgccaac
    10381 aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag cggcaggcct ccctttgccc
    10441 gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg atgatagttg ggttacttgt
    10501 gagcaactaa tgccaatgct gcccattgtg aaggtcaagg attttgacag cgctctaacc
    10561 ctggcgctga aagtggaaga cggcttgcac cacacggcaa ttatgcactc tcagaacgtc
    10621 tcacgactca accttgccgc gcgcgttatg caaacctcaa tatttgtcaa aaacggcccc
    10681 tcctgggcgg gtattggcgt cggcggcgaa ggctttacga cctttaccat tgcaacaccg
    10741 actggagagg gtacaacgtc agcacggact tttgcccgct cacgacgctg cgtgttaacc
    10801 agcggctttt caattcgcta gagaaacgga aatgaatact ttttcactgc agacgcgttt
    10861 atacagcgga ccaggaagtc tcaaggtact tagccgtttc tctcatagac acatctggat
    10921 tatttgcgat gcctttctgg cacgctcacc acttattgac acgttgcatc aggcgctgcc
    10981 cgacagtaat cgtctcagta tttttagcga aataacgccc gccccaacta tccagaccgt
    11041 ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat gtagttatcg gttttggcgg
    11101 cggttctgcc ctggacgccg caaaagcaat tgtctggttc ggtcgccagt gtggcattga
    11161 aattgaaacc tgcgtagcca ttcctaccac cagcggtatc ggttccgaag tgaccagtgc
    11221 ttgtgtcatc agcgatcctg agaaagggat taaatatccc cttttcgata atgcacttta
    11281 tccggatatc gcgatccttg atccatcttt ggtcgtcagc gtgccaccgg cgataacggc
    11341 caataccggg atggatgttt taacccatgc gcttgaagct tatgtttctc ctcgcgccag
    11401 cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt gtttttcaat acctgcccac
    11461 tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa atgcataatg catcgacgct
    11521 tgcaggaatg gcctttagcc aggccggact cggtttaaac catgcaattg ctcatcaact
    11581 cggcggacag tttcatctgc cgcatggact ggcgaatgcg ctcctgctca cggcagttat
    11641 ccgctttaat gctggcgatc cgcgcacagc caaacgctat gcccggctgg cgaaaacgtg
    11701 ccatttatgc ccggacaacg ccaatgacac cgccagcctg aatgcactta ttcaacatat
    11761 tgagcagctt aatacaacct gtacgctgcc gactcttgcc aatgcgctga aagaaaaaaa
    11821 agcagaatgg tcaatacgca taccagatat ggttcaggcg gcgctggcgg atgcaacgtt
    11881 gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca gaactgctcg aggagttgtt
    11941 atgaacgtcg gattaatctc ctgcgacgac cagaccatcc gcgagcgagt acaagcagca
    12001 ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata ttaagctaca agcgcgtgct
    12061 gacactttcc tggtgaatgc ggcggaatgc gaacccatgt tgaaagtaga tcaacagtta
    12121 atggcgcagc aagccagccg cctgcttcgc ggtgttcact atgcgatgaa agccaccggg
    12181 gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac gggcaataaa cgcactcaca
    12241 ccactgttac caccggatat ccgcctgcat attttgcccg acgtttaccc cgcaggagac
    12301 gaagtgctga ccatctggat ggcaactggt cgccgcgttc cacccgctgc attacccgtt
    12361 agcgtcggtg tggtggtcaa taatgttcaa accgtactta atattgccag agccgtcgag
    12421 caacagtatc cagtgacgcg ccgcacactc accgttaatg gtgcagtggc gaagccaata
    12481 acggtaaccg tccccatcgg catgtcttta cgtgaagtcc tggcgctggc gggcggcgca
    12541 acagtggatg ctcctggttt tatcaatggc gggccgatga tgggaagttt gatcacatcg
    12601 ctcgacacac cagtcagcaa aaccactggc ggcttgctgg ttttacccaa ctctcattca
    12661 ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg ccgtcgcgaa aactgtttgt
    12721 gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt taatcggtca cgaactggca
    12781 ccccatttat tagtacgtgc ggttaactac caacagctcg ccacgccgca actgttactg
    12841 acagcgctca cctgttcaga gtgcaacgtc tgcgccagtg tcgcctgtcc ggtaggaatt
    12901 tcaccaatgc gcataaaccg cctgcttaag caggaattac gcgcacaaaa tctccgctac
    12961 gaaggtgcct taaatcctgc agatcccatg gcaaattatc gactgattcc cgtaaaacgg
    13021 ctggtaacca gacttggctt aaccccgtgg tatcaggatg cgccattgtc tgaacaggtg
    13081 cctcagcccg aaaaagtgac gttattgtta cgccagcaca tcggtgccag cgcaattgcg
    13141 tgtgtacaaa aaggcgatcg cgtcgttcat gggcaatgtg taggtcaaat tccacacggc
    13201 acattaggtg cccccattca tgccagtatt gatggcatgg tcagcgatgt gacagaaaac
    13261 gcgattacgc ttgtgagagg ttaaccatgt cgaaagcggt aggtatttta gaacttagca
    13321 gtatcgcgaa agggatggaa accggcgacg ccatgctgaa aagcgccaac gttgagttac
    13381 tggtcagcaa aacgctatgc cccggaaaat ttctgctgat gctcggtggc gatgtgggcg
    13441 cggtgcaaca ggccatcaac agcggagcgt cccttgctgg tgaaatgctg gtcgacagtc
    13501 ttgtcctgcc gaatattcat cccagcctcc tttctgcaat cagcggactt aaccatgttg
    13561 aacagcaccg ggctgtcggc gtagtcgaaa cctggagtgt cgccgcctgc attgacgctg
    13621 ccgattgtgc tgtcaaagct gcaaatgtca ttcttgttcg cgtacatatg gcttttggca
    13681 ttggcggtaa atgctacatg gtggttgctg gcgatatttc agatgttaat aacgcggtct
    13741 ctgccgccag cgtgcgcgcg ggggaaaaag gtttgttggt ctatcggtcg gtgattgccc
    13801 gtccacatga agccatgtgg cgtcagatgg tggagggata atgacaactc agcacacaac
    13861 tgaacggatg attcaggaat atgtcccagg taagcaggtc acgctggcac atgtcatcgc
    13921 caatccaggg aaggatctgt ttaagaaatt gggtttacca gacaccattt ccgctattgg
    13981 tattctgaca attaccccca gcgaagcatc tatcatcgca tgcgatatcg ccacaaaatc
    14041 aggcgctgta gagatcggtt ttcttgatcg ttttaccggt gccgtagtgc tgactggtga
    14101 tgtttctgcg atcgaatatg cacttagcca ggttacccgt acgctgggtg aaatgatgcg
    14161 ctttaccgcc tgccccatta cgcggacctg acgttatgca acgtatgatg ctcatcggcc
    14221 ccagccagtg cggaaaaacg tcactcacgc aatgtctgcg tggcgaaaat attcattatc
    14281 agaaaacgca ggcaatcgtc tggtccccga ccaccattga tacaccaggc gaatacctcg
    14341 aaaaccgcag cctctacagt gcactgttgg tcagtgcctg cgaagcggac attatcgcgc
    14401 tggtgcttaa tgcggacgca ccgtggtcac ctttttcgcc aggctttacc tcgtcaatga
    14461 atcgcccggt cattggagta gtaaccaaag cagatttagc caacatggaa caaatatctt
    14521 tggtgaaatg ttggttacgg gaagctggag cgcacaacgt gttagtaacc agtgcggtta
    14581 ataaccatgg agttgcagaa cttttagcct tgctgcatac agaagaaggc tgtcgctaat
    14641 ttcgacaggt tatcagatgg gaatgatgac ggtgatacac agaaaagaat caatgagcaa
    14701 tgagggttgc cgggcaaccc tcattgaata aaacaggaat tacttcgctt cgccgttttt
    14761 catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg agagctcggc
    14821 atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc tggcgatgat
    14881 gccctggatt gcttcaacgc tcatcccagg cttgatgtaa tcgctcaggt tgtccagcag
    14941 caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt cacgttgcag
    15001 tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg aaggcttggt
    15061 agtttccatt tttatacccc tgataatgtg agagtcagat tcgtttaagc aaccgctatt
    15121 cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc ccttttcact
    15181 atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga agttcaggaa
    15241 ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat gcgccgtttc
    15301 catgttcagc cacacataac catagattgg tgtttccact accttcaggt catggtggtt
    15361 attgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat tggcaaaaac
    15421 cggctgcgga ttaccttcgt acagcgcatg aacaaagctc agcagtattt gctgcatcat
    15481 gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg ggcgcgtggc
    15541 ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa tggtctggat
    15601 accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa tcagcccacg
    15661 cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg ggcgttcgag
    15721 taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac ttttcgccag
    15781 ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca acattgccag
    15841 taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt cacctgtcgg
    15901 ggaaccaaca acaattgcca gcgtcacccc aatcaataat ccttgatacg gtttcttgcc
    15961 cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca tcagcggtaa
    16021 cgaaattaac tccagttgca gagcgataag acctaaaatc gaacccaaca ccgtaccgcc
    16081 aatacgttcg aaggcacgag ggacaacgtt accccagaac gagattggcc ccataatcac
    16141 caccatggtg accagcggcc aggtgctttc cgggatagta aacaggcgga taatgagaaa
    16201 agtgagcagg aatgccagcg cgacccgagt accatgcact atgcggtaat ggcggtatac
    16261 ccggatttca aacgggctta atgacttatc ggcacgcacg cagaaactcc gtaatgaaga
    16321 ttaaaagcgc tcataactca tctgtcggcc agaaggcgcg cctctgaatt atccgacgga
    16381 caaaccggga acagtgtaat ttcccggcag gagcgaaaaa attgaaaggc gcatcttact
    16441 cttttcgctt tcaaaaaaag agtggtcatc gcgttaacac accgccctga gatgaattag
    16501 tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca
    16561 ttgttcaaat aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag
    16621 aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc
    16681 gccaccgcat actgaccacc tgaaatttct gtcagaatga cgccctcact gttttcggga
    16741 agcgtaaagt tattcggtac cgtcacaacg gtgtcgcagc gtaatttttc ggcgggtgtt
    16801 tcatctggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgttatct
    16861 acccacatca tcaactgctc aaagcctttc tttaccgtct gttcccacgg gccaacgaga
    16921 tggaaacctg caacggtacg tttctcttcc tgcgtaatct cgtagttcat gacgcctcca
    16981 ttaacactgt ttttatatac agcatagttg caatattaaa accacaagga atgagtgttg
    17041 attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg
    17101 gtggaaataa tcactcaggc gggaaaacat gccgccttcc ccgacagatt ccagggtaac
    17161 cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg
    17221 atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg ctttgatatg
    17281 tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccta ggtcgatatt
    17341 ttctttatcg ccataccaga tgcgttccgt tccgaccttt ttcccacggt gcaaaatttg
    17401 caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc
    17461 ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat ctacagccga
    17521 agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt caacattcat
    17581 ggttttatcc cacaacaacc cgttacggtt ttgctgggtg ataccgttcc aggtgagact
    17641 tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac
    17701 agctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca ctgtttcaaa
    17761 atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg
    17821 ttgcccaccg gcaatatagt cagccagagc aacacaagcg tcatttccgg aatccacaat
    17881 taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga
    17941 agaaccgaca aacaccggat tatctttcgc ccacgcatcg cgccccacgg tgacaatatc
    18001 gtctggcgta atgcgatgac tatcgatagc gcgatccacg acataacccg tcatcagctt
    18061 tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagga tctgaccggt
    18121 ggtgtaatcc atcaataccc aggacccggc atgaatctct ggaggctgag gtgaaaaagg
    18181 aatgttttcc gccgcaaaac cagacgataa gttaaaaacg aacaaagaag cagcaataat
    18241 aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt tttacggaaa
    18301 tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc atttttatct
    18361 gacatgatct gttgccactc gctgccaaat tgtggcgcta aagctgatta gcacggtgat
    18421 atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga atgacggtaa
    18481 gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggcacgcacc ccgcgttaga
    18541 tcgccctgca cagttcgcag ccattcgcac cgatagcgaa ttcaatgtca tcggcgaacc
    18601 cgaagtcttt tactgcaagc ccgctgatga ctatttaccc cagccaggag ccgtattaat
    18661 taccggtatt accccgcagg aagcacgggc gaaaggagaa aacgaagccg cgtttgccgc
    18721 ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg
    18781 tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg
    18841 gagctggcag catgataact cgcgctggga tttgctggat gttatgcgtg cctgttatgc
    18901 cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga gctttcgcct
    18961 tgagcattta accaaagcga atggaattga acatagcaac gcccacgatg cgatggctga
    19021 tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac gtctgtttga
    19081 ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg ttccgcagat
    19141 gaaacccctg gttcacgttt ccggaatgtt tggggcatgg cgcggcaata ccagctgggt
    19201 ggcaccgctg gcgtggcatc cagaaaatcg caatgccgta attatggtgg atttggcagg
    19261 agacatttcg ccattactgg aactggatag cgacacattg cgcgagcgtt tatatactgc
    19321 aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata tcaataaatg
    19381 tccggtgctg gcccaggcga atacgctacg cccggaagat gccgaccgac tgggaattaa
    19441 tcgtcagcat tgcctcgata acctgaaaat tctgcgtgaa aatccgcaag tgcgcgaaaa
    19501 agtggtggcg atattcgcgg aagccgaacc gtttacgcct tcagataacg tggatgcaca
    19561 gctttataac ggctttttca gtgacgcaga tcgtgcagca atgaaaattg tgctggaaac
    19621 cgagccgcgt aatttaccgg cactggatat cacttttgtc gataaacgga ttgaaaagct
    19681 gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg agcagcaacg
    19741 ctggctggag caccgccgcc aggtcttcac gcccgagttt ttacaaggtt atgcagatga
    19801 attgcagatg ctggcacaac aatatgccga taacaaagag aaagtggcgc tgttaaaagc
    19861 gctttggcag tacgcggaag agatcgtcta atttgaagcc tccgccgctg gtacggcgga
    19921 ggtcaatgtt gagtcaggct ttttgaacgg tgatactcca cgctgcatcg ccaatctgct
    19981 gataatcggt gatggcatgc ccttcttctg ccgcccactg tgggattgct tcagttgcct
    20041 gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc agtgctgctt
    20101 ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt ttaatcgcca
    20161 tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg ctgcggtcga
    20221 acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag gccaatccag
    20281 ccctgccagg tcatcattgc agtcataacc agtccattac cgataaagca accacctgca
    20341 atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt tgtttgtgca
    20401 tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatga ccccacgaag
    20461 atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc gaccagaaat
    20521 tgcagaatat tggccgttgg ggaagtgatt cctaacccaa acatgcgtcc ggttgcttca
    20581 ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac aaagggatgc
    20641 cagcgttttt caaacagaat atgagcgatc ccagttcggc gcggcggtaa ggtcgcgact
    20701 ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac cagcaaaacg
    20761 gcaaccaacg gccaaacaga caaattaaaa gtctcagcaa tagagttatg ttcagtactg
    20821 tagtgctgca aggtttgatt taaaccactg gcatgtggag aacgcatcac cgcactcatc
    20881 accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc gcgataccag
    20941 gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaagatata cccacctata
    21001 acagtaccaa gccacgggaa cgcctcggct tcgtaagtca gtaggcccgc ctgaattaac
    21061 gcaaaaaccc ccacgctttg taccgaaatt gcaatcagca aggcgtaaaa catgcgatta
    21121 tttttcgcga tatacatatc gcgaaaacca cctgtcaggc aaaaacgccc acgctgcatg
    21181 acaaatccca g
//
LOCUS       DAZPLM010000060        20820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPLM010000060 DAZPLM010000000
VERSION     DAZPLM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.060"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(13..1872)
                     /gene="gss"
                     /locus_tag="ACHVC7_001859"
     CDS             complement(13..1872)
                     /gene="gss"
                     /locus_tag="ACHVC7_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR2115932.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2077..2943
                     /gene="yghU"
                     /locus_tag="ACHVC7_001860"
     CDS             2077..2943
                     /gene="yghU"
                     /locus_tag="ACHVC7_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR2115933.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2972..3053)
                     /locus_tag="ACHVC7_001861"
                     /pseudo
     CDS             complement(2972..3053)
                     /locus_tag="ACHVC7_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3066..3314)
                     /gene="hybG"
                     /locus_tag="ACHVC7_001862"
     CDS             complement(3066..3314)
                     /gene="hybG"
                     /locus_tag="ACHVC7_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR2115934.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3327..3668)
                     /gene="hypA"
                     /locus_tag="ACHVC7_001863"
     CDS             complement(3327..3668)
                     /gene="hypA"
                     /locus_tag="ACHVC7_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR2115935.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3661..4149)
                     /gene="hybE"
                     /locus_tag="ACHVC7_001864"
     CDS             complement(3661..4149)
                     /gene="hybE"
                     /locus_tag="ACHVC7_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR2115936.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4142..4636)
                     /locus_tag="ACHVC7_001865"
     CDS             complement(4142..4636)
                     /locus_tag="ACHVC7_001865"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR2115937.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4636..6339)
                     /gene="hybC"
                     /locus_tag="ACHVC7_001866"
     CDS             complement(4636..6339)
                     /gene="hybC"
                     /locus_tag="ACHVC7_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR2115938.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6336..7514)
                     /gene="hybB"
                     /locus_tag="ACHVC7_001867"
     CDS             complement(6336..7514)
                     /gene="hybB"
                     /locus_tag="ACHVC7_001867"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR2115939.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7504..8490)
                     /gene="hybA"
                     /locus_tag="ACHVC7_001868"
     CDS             complement(7504..8490)
                     /gene="hybA"
                     /locus_tag="ACHVC7_001868"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR2115940.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8493..9611)
                     /gene="hybO"
                     /locus_tag="ACHVC7_001869"
     CDS             complement(8493..9611)
                     /gene="hybO"
                     /locus_tag="ACHVC7_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR2115941.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9800..10087)
                     /gene="yghW"
                     /locus_tag="ACHVC7_001870"
     CDS             complement(9800..10087)
                     /gene="yghW"
                     /locus_tag="ACHVC7_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR2115942.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10206..11093)
                     /gene="yghX"
                     /locus_tag="ACHVC7_001871"
     CDS             complement(10206..11093)
                     /gene="yghX"
                     /locus_tag="ACHVC7_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR2115943.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11250..12290
                     /gene="gpr"
                     /locus_tag="ACHVC7_001872"
     CDS             11250..12290
                     /gene="gpr"
                     /locus_tag="ACHVC7_001872"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR2115944.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12330..12824)
                     /locus_tag="ACHVC7_001873"
     CDS             complement(12330..12824)
                     /locus_tag="ACHVC7_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR2115945.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13015..13899
                     /gene="yghA"
                     /locus_tag="ACHVC7_001874"
     CDS             13015..13899
                     /gene="yghA"
                     /locus_tag="ACHVC7_001874"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR2115946.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14059..14484)
                     /gene="exbD"
                     /locus_tag="ACHVC7_001875"
     CDS             complement(14059..14484)
                     /gene="exbD"
                     /locus_tag="ACHVC7_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR2115947.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14491..15225)
                     /gene="exbB"
                     /locus_tag="ACHVC7_001876"
     CDS             complement(14491..15225)
                     /gene="exbB"
                     /locus_tag="ACHVC7_001876"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR2115948.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15477..16664
                     /gene="metC"
                     /locus_tag="ACHVC7_001877"
     CDS             15477..16664
                     /gene="metC"
                     /locus_tag="ACHVC7_001877"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR2115949.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16804..17463
                     /gene="yghB"
                     /locus_tag="ACHVC7_001878"
     CDS             16804..17463
                     /gene="yghB"
                     /locus_tag="ACHVC7_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR2115950.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17503..18402)
                     /gene="yqhC"
                     /locus_tag="ACHVC7_001879"
     CDS             complement(17503..18402)
                     /gene="yqhC"
                     /locus_tag="ACHVC7_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR2115951.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18596..19759
                     /gene="yqhD"
                     /locus_tag="ACHVC7_001880"
     CDS             18596..19759
                     /gene="yqhD"
                     /locus_tag="ACHVC7_001880"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR2115952.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19864..20691
                     /gene="dkgA"
                     /locus_tag="ACHVC7_001881"
     CDS             19864..20691
                     /gene="dkgA"
                     /locus_tag="ACHVC7_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR2115953.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5122 a         5607 c         5223 g         4868 t
ORIGIN      
        1 ctacaacatg tattactttt tcaccacaat taacggttca atatcactct cttttttgat
       61 gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag ttgccgccca cggtgaaggt
      121 acatacctga atgtatttac cgtccacttt cggcaaacac cacagttgct gatagatgtt
      181 tttctgctcg gcaaatttac cgctggtttt gtccagcacc tcttcatgat ggctgaccag
      241 gtcgatattg ctaccacagc gaccggcaat cggtttcact gcgtaacctg ttttcaccaa
      301 ttcatcatta acagtgaaat cggtatccag caggtaacga tggtgcggga acagcgacca
      361 gaggatcggc agaatcgctt tgttgccggg gatcaccgtc cacagcggct caaagactag
      421 cacttccggg cgcagcaata cgtcgataag acgcacttcg ttttgcggat gaccggtacg
      481 gattggcacc gcagcaaact cacggtcgct aacttcacga atctgatcaa acgcggtttc
      541 ccacgcccag gttttccaca cgcagttaac cagtcgccct tccccatcaa tcagttgccc
      601 ggcagcatcc cagcccagtt catccaatcc acgcaagata cgcgtttcaa agcccgcctg
      661 gtgcagcgcc tgctccataa actgcgcgtg atagttttcc tcgatatctt tgtcctgcat
      721 gatatggaca aacggacgtg cacgactgtg tttccaggca ccagccaatt cgttaatcag
      781 cccttccgcc ggattgaagc cgttgccttt atagccctgc tccgcccaac gttcgaggat
      841 caagcccgct tcggtatgac aggaggcgga gtcggcgttg tactcgtaaa ccttcaggcc
      901 acgctcatcc atgcagaaat ccatacgacc agtgatcata tggtgacggc gacgctgcca
      961 ggagagacgc aaacgtggcc agaggatttt cgggatgtcg aacagcgcca gcaggttgtc
     1021 atctttcagc accttgtcgg ttgcgtgaag atacatcagg tgcagctcgt tggtggcttt
     1081 aattagctcc tgctcggcac tctcggtaat ggtgtagtaa tgataaggat cctgattgat
     1141 cacctgaccg ttggcctgca cataggcgtt ttgcagcgga tctttttcat ccagccattt
     1201 accgtcaaac tggcctttgt tttccaggcg cgctccgctg attttcagca gctcgcctgc
     1261 aatttccggc tgcggtaagc tgtattcagt atcttccgtc tggatcatcc agcccagaat
     1321 ggtggtgtca tcaaaagtgt ctttcagggt atagcagccg ttttcgacca ccatctccag
     1381 ctcacgcgtc cactgttgcc cttgcggcaa cggggaatga atcacgttct gttccgcaat
     1441 acggactttg ttgccatgca attgggtaat gatggcgaca tggccagtgt ctttaaattc
     1501 accgccttta tcccagataa gaagcgcacc cgcgaccggc gcacgcggcg agccgttagg
     1561 aaatgcctgc aatggcagga tgttgtcatt aaccacttct cgcaggaagc gcagcgagaa
     1621 aatctcccac gccataccca cgtcagtaaa gaccacaccg taattcagaa agagaaaacg
     1681 gcgagcaaat tcaacgcatt gccacttgtg gcccatatat tcgtcgtcga tatagctacg
     1741 gaatacggcg tcatcttcgt attcctgcgg atcgagagaa ctgtaatctg aagagtagat
     1801 tgctacccca cctggggcgt agcccaataa tgtcccgaac ggggcatcct ggctggtcgt
     1861 tcctttgctc atcactttac ctttaacaat acaacctaag cagttggcgt aattgttgtg
     1921 ctctgattac ctgctcagcg acattaaccg gacagaggtc agactaatca tacacctcat
     1981 cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg tacagcaagt gacctgctac
     2041 actgcttcaa cactaccact cagaaggcaa ctcactatga cagacaatac ttatcagccc
     2101 gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt tcgccaatat caatcgcccg
     2161 gtttctggtc cgacgcatga aaaaacgctg cccgttggca aacacccatt gcaactttat
     2221 tcgctgggaa cgccgaacgg tcagaaagta acgattatgc ttgaggagct gctggcgctg
     2281 ggcgttactg gtgcagagta cgacgcctgg ctgattcgta ttggcgatgg cgatcaattc
     2341 tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc cggcgctgcg cgatcatacg
     2401 cataatccgc cgatccgcgt gtttgaatct ggttcgatcc tgctttatct ggcggagaaa
     2461 tttggctact tcctgccgca ggatttggca aagcgtactg aaacgatgaa ctggctgttc
     2521 tggttacagg gcgcggcacc gttcctcggc ggtggttttg gtcactttta ccattacgcg
     2581 ccgatgaaaa ttgagtacgc catcaaccgc tttaccatgg aagccaaacg tctgctcgac
     2641 gtgctggata agcaactggc gcagcataag tttgttgcgg gcgatgagta caccattgcg
     2701 gatatggcga tttggccgtg gtttggcaat gtggtgttag gcggcgtgta tgatgccgca
     2761 gagtttcttg atgcgggcag ttataagcat gtacaacgct gggcgaaaga agtaggcgaa
     2821 cgtccggcgg tgaaacgtgg gcgtattgtt aaccgcacca acggaccgct gaatgagcag
     2881 ttgcatgagc gccatgacgc cagtgatttc gagacgaata cggaagataa gcgtcagggg
     2941 taagggttgg tgttcgtcgc agcaagccat ccaggccgga taaggcgttc acgccgcatc
     3001 cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacgaaagct
     3061 aatcatcacg cgctggtaat gtcgtaatcc atttggcgca gtgcgtctaa tgtggctttg
     3121 gcttcatctt cgtcgatgat gctcatggca aatccgacgt gtaccagcac ccactggccc
     3181 agtagatcgg cagggttacc ttcacaaatc agggcgatat tcacgtcgcg cttgatacca
     3241 catacttcaa cctgcgcaag ctggtgaata tcttcaccga cagccagcac ctggcctgga
     3301 acgccaatac acattattaa ctccggttat tcaacttcaa tacttttgac gatcagcgaa
     3361 tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc atcgtgctga
     3421 tgaatctcca ccacctggct gcaatcccag caccaagcct gggcgggttt atagacgata
     3481 tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact aaaacggacg
     3541 gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt gacgcgctta
     3601 acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca aagagacaac
     3661 tcatgcattt tcgccactcc tgcgaccaaa cagcagggcg cgacgccctg cgtgtggtac
     3721 atccggattc gtgactggca gcgaaaggat cattcgtgcg cagtcatccg tcaggcgttg
     3781 gccctcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg agagatattg
     3841 cgaaacaccg tccagttcgc caacggtaaa ggtcatagtg ccatacggca gttgcagacc
     3901 aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga agataactgc
     3961 actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca gcgtaaaatc
     4021 agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg aacgccgggc
     4081 aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac ctgctatctc
     4141 ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc agcgcagcca
     4201 gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag ccgatgtgtg
     4261 gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac tcaccggtga
     4321 agcgcagggc cgacaatacg tcggccaggc caagctgatg cggagagatt ttgttggtaa
     4381 acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggcgtg ttctttttcg
     4441 acacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca agcagctcca
     4501 ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat cgttgctcta
     4561 acgcttcgac aatccgcaca ccgatggctt catcggtcag caaaatattg ccgaccccta
     4621 agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt cggcatccac
     4681 tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga atggtacgca ccacttccag
     4741 cggtttattc ggatcagcaa ccggtgtacc caccagcgac tgctcgtaag gaccgacgtc
     4801 atcattgaag ttacgcggac cagagttcca ggttgatgga acaactgcct ggtagttact
     4861 gataataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg cttcgaggaa
     4921 gccaacacct ttgaactcac ccgttgccgg aatgttcggt ttcacaaagg tggtgtgatc
     4981 gcctttgccg atattggtga tcagtgcact gtattgattt tgcaggatat cctgcaattc
     5041 gcagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct gcgccacttc
     5101 cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt tgttctgggt
     5161 agattcgcga cctgccgcca gtttcaccag catattagcc agcggaccca cttctaccgt
     5221 tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag accaaccatc
     5281 ataagccgga atggtggtac cttcccacgg tgcctgcggc gcttcgtctt tataccagga
     5341 gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag aagtgatcgg
     5401 acgatacgag gacagatccg cattctcaat gtagccgccc gggaacagga agctgccgtt
     5461 tttactgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt taccgcgcgt
     5521 cagccattcc gggtagaacg cggcaataac tgcggtatca accttataaa cctgttcaac
     5581 aaagtcgctc agcttgtcga tgaaagactt gatgtacatc aggcgctcaa ggttcagcac
     5641 gcccaaaccg tcgaggttga tcgggttcgc gacaccacct accgccaggt tctgaatgtg
     5701 cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc actccagcgc
     5761 ctgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca tcgccgggtg
     5821 acccccgtag ccattggcga aaatacccaa ctgaccgctg gcaaccagat ctttgatctt
     5881 gttctgaact ttagtgaact cttccggact gttcagatgc caggtcgaaa cgcctttcag
     5941 catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca cccagtccag
     6001 cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag ccagaatgat
     6061 gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt ctgccgcacg
     6121 aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca caatcatcca
     6181 tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca tggtaccgga
     6241 agcccatgct ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta aatgcccctc
     6301 aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc ctcatgacga
     6361 ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag tacgatgtaa
     6421 gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca ggtcgggaag
     6481 taggcgtaac cgccgcccgg gttgaatgcc accagcgaat aggtcagacg ccaggttgca
     6541 caacctaaca gtgcgctcag tgctgacagg aacagcatgc gggaatcatt acgcagcttc
     6601 gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca gaacatcacg
     6661 gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag ctcgccaaag
     6721 cgcagcacga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt gacgaacaga
     6781 ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagcgaacc ttcaaagatg
     6841 acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa catttcatag
     6901 ctctgccaca acggatgcac cttgtagccc gccgagatca tcagtgaccc cattgaagac
     6961 tggtgcatgg tcggcaacag cgcgccaagt gcaatgatga agaacataac cttgttcaga
     7021 cgcttgagag aaaccttcca gcccagacgt tcaaacagtg ccggagcaaa ctccagtgcc
     7081 atcacgccga tatagatggt catacagacc gccgtctcga acagtaccga gttcacgttg
     7141 aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc gatagtgatc
     7201 gacaagccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg caccagcgga
     7261 tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc gccacaggca
     7321 aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa gccgccgttc
     7381 aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag catacagatg
     7441 acgattaacg gtccaaaaat catgaccggt ttactgatga ttttgccgcc cagcggttgt
     7501 ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt tgcgacgaac
     7561 cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat acagggtgtg
     7621 ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca ggtcgagatt
     7681 ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt tctcgccgta
     7741 cagatgcgga taatatttcg gcaccgtatg caggtaagtg tcgccagatt tcagcgtctg
     7801 acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg cctccgccat
     7861 cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta cgcagccagg
     7921 cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc acttatgcag
     7981 cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg cgaccatgca
     8041 gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt tcggatcttt
     8101 tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt gcatacactg
     8161 tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgacccctg tgccgctggt
     8221 ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg accaggtctg
     8281 ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga cgcaagcctg
     8341 gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa ttggcgggcg
     8401 gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg ccccgcagga
     8461 ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc agcgttatct
     8521 ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc aaccagccca
     8581 accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc tttagcgtta
     8641 acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg atggatgcct
     8701 ttatggaagc cgataccttc ttcgttacag ccatagcatg ggtgaccaat cgctaccggc
     8761 cacacgccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt ttctggccct
     8821 ttacagccga ggtggtacag gcaccagcct tcacggtggc cttcatcacc gaactctttg
     8881 gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat cagacggcca
     8941 taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta agtgatgatg
     9001 tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat attgataacg
     9061 gttttgcctg gcagaacttc ttgcaggctg actgcgccag ttgggttaac tccagctgcg
     9121 gcaacaccgc cccacgcaga gcaggaaccg atagcgataa tcgctgctgc gccttctgcc
     9181 gctttgcgga tgtgatccac aatcggctca ccagcaacca tgcaataaat accgttatct
     9241 tttaatggga tggaaccatc cacaaccaac acatactgcc ctttgtactt ctcaagtgcg
     9301 ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc gtgatactcc
     9361 agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg aagcagagat
     9421 tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg ctgcgggtta
     9481 gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt ggcggctaat
     9541 gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat gagggtgtta
     9601 tctccagtca tttatagtta ttccgttgcg aagacctggc atatattttg cctcaatcgc
     9661 aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt atcgatatca
     9721 tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt aattaaagca
     9781 agataatacg tatgtttgat caattttcga ggcgataacc ggccataaaa aagcgcaacg
     9841 tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc agtccatagc
     9901 ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc ttttcgtaca
     9961 tccggtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat tttgtcacat
    10021 taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta ccaaaatggt
    10081 tattcatcgt tcacctcatt cgctatatat cgccatatat agcgatattt taggcaaaga
    10141 acgacttttg cgcacgctat tttttcatgc ttgtttaaga cgtaatccag taagagataa
    10201 acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc aagatcggcg
    10261 gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac ccccggatag
    10321 atatacgcct cataaacctt attattggct ttcaacgccg cctcgtaagc aggccagccc
    10381 tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc aatcttcgcc
    10441 acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag ttccggatac
    10501 gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat acccactttg
    10561 cctgccgctt gtggatagcg ttgcataaac tcaattgcgg caaagaaatc attcatcagt
    10621 ttggttggat caacctgctg ttgcagctca cgacctttat catcatttcc cggataacct
    10681 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgc cactcgccgt
    10741 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac cacggctggc
    10801 gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt
    10861 ggcgaaggat acatgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc
    10921 gccagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc
    10981 gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg agcgtaatag
    11041 tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca
    11101 ctcctcagtt ggtgtttttt taagcaaagc ataagcacgt gtttttgccc agtttttcgt
    11161 cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag aaacattgtt
    11221 tcgaaattga acggtggaaa ggagaggtca tggtctggtt agcgaatccc gaacgttacg
    11281 ggcagatgca ataccgctat tgcggaaaaa gcggtttacg cctgcccgcg ttatcgctcg
    11341 gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgcg atcctgcgta
    11401 aagcgtttga tttgggcatt acgcactttg atttagccaa caattacggg ccgcctccag
    11461 gaagcgcaga agagaacttt ggtcgcctgc tgcgggagga ttttgccgct tatcgcgatg
    11521 aactgattat ctctaccaag gctggctacg atatgtggcc cggcccttac ggctctggcg
    11581 gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg gggcttgagt
    11641 atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa gaaaccgcct
    11701 ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc tcctcttact
    11761 cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt ccgcttttaa
    11821 ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc ctgctggata
    11881 ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag ggattgctga
    11941 ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa gggaataaag
    12001 ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg cgcttattga
    12061 atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc tggttgctga
    12121 aagatgatcg cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa ctagaggaga
    12181 acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag attgatcagc
    12241 atatcgccga tggcgagctg aatctgtggc aggcgtcgtc cgataaatga cctgttaata
    12301 acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag tcggtcaaga
    12361 tagcccatca caaatgcaga aagcacaaac gtcagatgga taatgacgta ccacatcagt
    12421 ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat ggaagaaatt
    12481 gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt ccccagccag
    12541 ttcagcttct ctttgttctc ggagatatcc agctgtgaga cgaaattctc ataaccggaa
    12601 aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga cagcaacacg
    12661 aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat aatctcctgg
    12721 aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac gggggcaagc
    12781 agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc ctgtcgtgta
    12841 gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc aaatcaacat
    12901 atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt tcccttatca
    12961 acgcatcgag agcgtctacc cttaaaggac gttgaacctg aagggagaaa aacgatgtct
    13021 catttaaaag acccgaccac gcagtattac actggtgaat atcccaaaca gaaacaaccg
    13081 acgccaggca tccaggcgaa gatgacaccg gtaccggatt gcggcgagaa aacctatgtt
    13141 ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc cggaataggt
    13201 cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag ttatcttccc
    13261 gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg acgcaaagcc
    13321 gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt tcacgaagcg
    13381 cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca ggttgccatt
    13441 ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat taacgttttc
    13501 gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc aagtatcatc
    13561 accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta tgcggctacg
    13621 aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga gaaaggtatt
    13681 cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc cggcggacaa
    13741 acgcaggata agatcccgca gtttggtcag aaaacgccga tgaagcgtgc ggggcaaccg
    13801 gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta cgtcaccgca
    13861 gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg gttgtgaaaa
    13921 gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc cttatccggc
    13981 ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc
    14041 gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt
    14101 caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt
    14161 tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc
    14221 cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt
    14281 caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa
    14341 gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac
    14401 caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag
    14461 gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg
    14521 aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac
    14581 ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt
    14641 atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc
    14701 tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa
    14761 gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat
    14821 ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc
    14881 cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc
    14941 cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc
    15001 ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct gctcgcgctt
    15061 aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg cccaagtgac
    15121 tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata
    15181 cataccccag acggaaaggt ccgtctgcat caaattatta cccactgtgt atctccagga
    15241 cgcaagtcac aaaatctgcg cataataata tcaaaatgac gtcgaattga tagtcgttct
    15301 cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa
    15361 aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc
    15421 ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgagatgg
    15481 cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactctcg
    15541 gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt ctttgacagt gtggaagcca
    15601 aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt
    15661 taacccattt ctccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgtgc
    15721 tatttccctg cggggcggca gcggttgcta attccattct tgcttttgtc gaacagggcg
    15781 atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc
    15841 tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta
    15901 agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccaggctcc atcaccatgg
    15961 aagtccacga cgttccggcg attgttgccg ccgtacgcag tgtggtgccg gatgccatca
    16021 ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat tttggcatcg
    16081 atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg atgattggca
    16141 ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaacgcctat ctgatgggcc
    16201 agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc
    16261 gtttgcgtca acatcatgaa agcagtctga aagtggctga atggctggca gaacatccgc
    16321 aagtagcgcg agttaaccac cctgctctgc ctggcagtaa aggccacgaa ttctggaaac
    16381 gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa ctcagtaatg
    16441 aagagctggc gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg
    16501 ggtatgaatc gttgatcctg gcgaatcaac cagaacatat cgccgccatt cgcccacaag
    16561 gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg
    16621 atctgattgc cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga
    16681 caattttgca gacatttaat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt
    16741 cccggacgaa tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag
    16801 tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg
    16861 gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa
    16921 aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca
    16981 ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac cgccgcagca
    17041 agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg
    17101 aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac
    17161 cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg
    17221 ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta
    17281 agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg
    17341 ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg
    17401 ttaaccgctg gcttgttagg cacgctgttt gtagtgatta aaaaaaaata ctgtaacgcc
    17461 tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc
    17521 ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacggct
    17581 aaattgcaat gcgctttcat agtcgacgcg catcgctgct gcgctggcct tcatgccgtc
    17641 atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga
    17701 ggtgctggtg acagacttaa aattatgatg gaacgccgat acgctcatgt tggcttctgc
    17761 cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg
    17821 gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg
    17881 tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc
    17941 cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa
    18001 cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat
    18061 gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaatc ctgccagcgg
    18121 cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta
    18181 ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa
    18241 gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg tctcgccata
    18301 caacaaacgc acatcgggca acagttctga caaactattt tctttatttt tcagtttatt
    18361 aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg
    18421 aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa
    18481 taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa
    18541 aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa
    18601 caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt
    18661 acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggcggcg gcagcgtgaa
    18721 aaaaatcggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt
    18781 tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg
    18841 cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa
    18901 atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac
    18961 gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac
    19021 cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc
    19081 gttccattct gcccatgttc agccggtatt tgccgtgctc gatccggttt atacctacac
    19141 cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca
    19201 gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct
    19261 gacgctaatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc
    19321 caacgtcatg tgggcggcga ctcaggcgct gaacggtttg attggcgctg gcgtaccgca
    19381 ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc
    19441 gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgaga ccaagcgcgc
    19501 taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg acgatgagcg
    19561 tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca
    19621 cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga
    19681 gcacggcatg acccaactgg gcgaaaatca tgacattacg ttggatgtca gccgccgtat
    19741 atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc
    19801 agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa
    19861 cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga
    19921 ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa
    19981 gtgggttatc gctcgattga taccgccgcg gcctacaaga acgaagaagg tgtcggcaaa
    20041 gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac
    20101 gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat
    20161 tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca
    20221 tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac
    20281 ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac
    20341 cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa cgcgacacac
    20401 aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag
    20461 aaagtcattc gcgatctggc agataaatac ggcaaagccc cggcgcagat tgttatccgc
    20521 tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc acgtattgcc
    20581 gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga aattgcaaaa
    20641 ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta acatgcaaat
    20701 tctcccggtg acggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc
    20761 gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc
//
LOCUS       DAZPLM010000061        20730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPLM010000061 DAZPLM010000000
VERSION     DAZPLM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.061"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..705
                     /locus_tag="ACHVC7_001882"
     CDS             <1..705
                     /locus_tag="ACHVC7_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2115954.1"
                     /translation="NKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
     gene            702..2153
                     /gene="djlC"
                     /locus_tag="ACHVC7_001883"
     CDS             702..2153
                     /gene="djlC"
                     /locus_tag="ACHVC7_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR2115955.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2213..3883)
                     /gene="hscC"
                     /locus_tag="ACHVC7_001884"
     CDS             complement(2213..3883)
                     /gene="hscC"
                     /locus_tag="ACHVC7_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR2115956.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(3999..4934)
                     /gene="rihA"
                     /locus_tag="ACHVC7_001885"
     CDS             complement(3999..4934)
                     /gene="rihA"
                     /locus_tag="ACHVC7_001885"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR2115957.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5052..5777)
                     /gene="gltL"
                     /locus_tag="ACHVC7_001886"
     CDS             complement(5052..5777)
                     /gene="gltL"
                     /locus_tag="ACHVC7_001886"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR2115958.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5777..6451)
                     /gene="gltK"
                     /locus_tag="ACHVC7_001887"
     CDS             complement(5777..6451)
                     /gene="gltK"
                     /locus_tag="ACHVC7_001887"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR2115959.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6451..7191)
                     /gene="gltJ"
                     /locus_tag="ACHVC7_001888"
     CDS             complement(6451..7191)
                     /gene="gltJ"
                     /locus_tag="ACHVC7_001888"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR2115960.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7361..8269)
                     /gene="gltI"
                     /locus_tag="ACHVC7_001889"
     CDS             complement(7361..8269)
                     /gene="gltI"
                     /locus_tag="ACHVC7_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR2115961.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8724..10601
                     /locus_tag="ACHVC7_001890"
     CDS             8724..10601
                     /locus_tag="ACHVC7_001890"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR2115962.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10679..12217)
                     /gene="lnt"
                     /locus_tag="ACHVC7_001891"
     CDS             complement(10679..12217)
                     /gene="lnt"
                     /locus_tag="ACHVC7_001891"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR2115963.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12242..13120)
                     /gene="corC"
                     /locus_tag="ACHVC7_001892"
     CDS             complement(12242..13120)
                     /gene="corC"
                     /locus_tag="ACHVC7_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR2115964.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13210..13677)
                     /gene="ybeY"
                     /locus_tag="ACHVC7_001893"
     CDS             complement(13210..13677)
                     /gene="ybeY"
                     /locus_tag="ACHVC7_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR2115965.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13674..14714)
                     /locus_tag="ACHVC7_001894"
     CDS             complement(13674..14714)
                     /locus_tag="ACHVC7_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR2115966.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14867..16291)
                     /gene="miaB"
                     /locus_tag="ACHVC7_001895"
     CDS             complement(14867..16291)
                     /gene="miaB"
                     /locus_tag="ACHVC7_001895"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR2115967.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16437..17612
                     /gene="ubiF"
                     /locus_tag="ACHVC7_001896"
     CDS             16437..17612
                     /gene="ubiF"
                     /locus_tag="ACHVC7_001896"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR2115968.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17766..17840)
                     /locus_tag="ACHVC7_001897"
     tRNA            complement(17766..17840)
                     /locus_tag="ACHVC7_001897"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17806..17808),aa:Gln,seq:ctg)
     gene            complement(17877..17951)
                     /locus_tag="ACHVC7_001898"
     tRNA            complement(17877..17951)
                     /locus_tag="ACHVC7_001898"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17917..17919),aa:Gln,seq:ctg)
     gene            complement(18000..18076)
                     /locus_tag="ACHVC7_001899"
     tRNA            complement(18000..18076)
                     /locus_tag="ACHVC7_001899"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18040..18042),aa:Met,seq:cat)
     gene            complement(18092..18166)
                     /locus_tag="ACHVC7_001900"
     tRNA            complement(18092..18166)
                     /locus_tag="ACHVC7_001900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18132..18134),aa:Gln,seq:ttg)
     gene            complement(18201..18275)
                     /locus_tag="ACHVC7_001901"
     tRNA            complement(18201..18275)
                     /locus_tag="ACHVC7_001901"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18241..18243),aa:Gln,seq:ttg)
     gene            complement(18299..18383)
                     /locus_tag="ACHVC7_001902"
     tRNA            complement(18299..18383)
                     /locus_tag="ACHVC7_001902"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18347..18349),aa:Leu,seq:tag)
     gene            complement(18392..18468)
                     /locus_tag="ACHVC7_001903"
     tRNA            complement(18392..18468)
                     /locus_tag="ACHVC7_001903"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18432..18434),aa:Met,seq:cat)
     gene            complement(18848..20512)
                     /gene="asnB"
                     /locus_tag="ACHVC7_001904"
     CDS             complement(18848..20512)
                     /gene="asnB"
                     /locus_tag="ACHVC7_001904"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR2115969.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20466..>20730)
                     /locus_tag="ACHVC7_001905"
     CDS             complement(20466..>20730)
                     /locus_tag="ACHVC7_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115970.1"
                     /translation="APVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAK
                     SLAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5046 a         5208 c         5374 g         5102 t
ORIGIN      
        1 aataaggaag aacaatatct tctttttgcg ctttctgcgc ccatggaaat acttaatcag
       61 agttgcaaac ccgcgcatga ctcgccaaaa atgtatactg ggataaaaga attcgatctc
      121 tcatcatcct ggggaataaa taatcgtgat gatttaattc agactattta tcagatgact
      181 gatgatggtc atgccaatga tttagctggc ttatatctga catggcaacg ctcttcccct
      241 gaagagtgga aagcattaat tgctggtggc tcggagagag ggttgattta tacccaattc
      301 gttgcccaaa ccgctatgtg ctgtggagaa gggggaatca aagcctggga ttatgttcga
      361 atggggtttc tgtcacgggt cggtgtgctc aacaactggc tgacggaaga agagagttta
      421 tggctgcaat cgcgcgtcta tgcacgagct caccattact atcacagctg gatgcactat
      481 ttttctgcct attcactagg gcgtctctac tggcaatctt ctcagtgcga agacaacgct
      541 tcgctacgcg aggcgctaac cctgtacaaa tacgatagcg ccgggagtcg gatgttcgaa
      601 gagctggcag cgggaagcga ccgtttctat gccacgcttc cctggcaacc attaactgtc
      661 cagcctgaat gcccggtaac gcttaaggat gtgagcgacc tatgaaaaca tgttggcaaa
      721 tcctcgaaat tgaaagcacg acgcaaatag acattatccg ccaggcttat cttgctcgct
      781 taccgttgtg tcatcccgaa accgatccgc aggggtttaa agcattacgc caggcctatg
      841 aagaggcgct gcgactggcg gtaaatcctg tcgaggaagc agatgatgaa gaaaaagatg
      901 ccaccgctga acatgaaata ctacgtgcat tcaggacatt actggattca gaaagtgatc
      961 gttttcagcc ctccgcctgg cagaaattta ttcagcaatt aaatacctgg aacatggagg
     1021 atgtcgatca attacgctgg ccgctgtgtg caatcgctat agaagcgcga tatctttcat
     1081 taaattgtgc ttctttgttg gcagagcgtt tgaactggca ttcgtttaat gacagcgaag
     1141 gaatggatga ggaagaaagg gaggcttttc ttgaggccat tcaggctggt gattgtttcg
     1201 atttccttag ccttctggaa tatcccgtcg cgttacagaa ccagactgtt gaatattact
     1261 tcgcgctgga acgttgctgc cgttaccatc ctgactatgt cactgcgttt ttggcgatgg
     1321 aaggaccgtg gttcattcct gatgatgcaa agttacatcg caaactgttg cgctggtaca
     1381 gctcggtgca aacaggtatg gcggaactca ttcctgtagc caaacagtgg caagcggaag
     1441 aaccagaaag cgaagatgcc cggtattacc agtgtgcaca acgtttgtac tgcggcgagg
     1501 gggaaagcct gcttgccgat ctctgcgcgt actgggaaag ttacccatct acacaagctg
     1561 ataatttgtt gttgcagtgg agcaagcgtc attgcccgga ttatttcgcg ttattagtga
     1621 tggttatcga agcgcgaagc atggtagatg cgcaaggtaa accgttgaaa tatgttcctg
     1681 gtgagagcgc ccggacgcgg ctgttatggg cggagatttt acatagcgga aaattatcgc
     1741 cgttaggtca atcgtttatt gagtcgttat tcttcaagcg caaagcatgg gcgtggtgga
     1801 aatcgagagt cggtagcgag acagagcaag attcaccgtt actggatttg tatcgggtag
     1861 cggaacaggt tgtgctggaa gcgttcccga aacaagagat gctggcccgt cttaatacaa
     1921 ggctggaagg tggagatgct catccattag aggccattat cacccagatg ctgttgacga
     1981 aagtgaaact cgagccggag gatgaagatg tcgatgagcc aacacctgaa aaccatgaag
     2041 aaaaaaatga tgaggatgaa aaaccacaga gcattaccag tattatcaaa atcagcttaa
     2101 cggtgctgat gataggttat gcgctcggaa aaatcgcgat gttgtttagc tgaggatgtg
     2161 ttttttgcgc cgatgccgga tgcagcgttt atgccgcatc cggcagccgt gtttaaggga
     2221 tctcaatagc caaataatcg ctcgcccgtc gacgaacttc atcgacgcgg gccatatcgt
     2281 ttgactgcaa cgccgcatca aaatcggtga tcacccggcc aatctcttct cgctcgtcac
     2341 ccagcgcccg cgcccacaac tcttccagtt tggccttaaa ggtgcggttg attagcatat
     2401 cgcgcggata aattttcaat gcggataacc gcgtccgact ctcttcaatc tgctgcgcgc
     2461 tcaatgttac cgggctatga ttaatgactc tggacttaac gctgccatct tccagaagca
     2521 cgtcaacttc aagcaagccg ttgatatcat aactaaagcg aatatcaatc gactgataag
     2581 ccccggtttt cttcaacggc acatcgaagg attcaaccag aatgttattt ttaactttgt
     2641 ggttttctcc ctgatagacg ttaaccgtaa tagaatcctg ttccgggtgc atggtcgagt
     2701 aagtttctac acgcgacacg ggtactgtgg tgtttcgttc aataattggc gagaaaatgc
     2761 cggaaacgcc ctggcggtta acttcaacgc ccaacgagta agggcaaata tcagtgagga
     2821 tcacctcttc aatatcttca ctgcgtaagc ggcaggcggc ctggattgct gcgcccagcg
     2881 cgacaatggt gctcgggtcg taactttgat acggtaattt gccaaacaga cgcacggcga
     2941 ttcgctgcac cagcggcatt tgtgacgcgc cgccaaccag caccagactg tcgatttgac
     3001 tcggcttcag acgtgcatcg cgcaacgcct gttcaatcgg cactcgcaag cgattgagca
     3061 gcggcaacca caaatcttcc agttcgttct cgtaaaattc gcactcccgc gtttcatcct
     3121 ggtactgcca gcgaatgtgc aatggcgatt gattgctaca ttttgccgct tccacacagg
     3181 cgtacaaggc tgccagttca ctctcgttaa gcgtggtcct ggcgacatcc gcgcgcttta
     3241 aaacgtcatc gaccaacata tgggtaaaat cttcgccacc gaggaagtta tcgccagcgg
     3301 aggcgtgaac ttcaatcacc ggcgtggcgt attcgagcac cgtgacgtca aacgtgccgc
     3361 caccgagatc aaaaaccagc gagcgggtat tttgttgcgt atgcaggcca tatgccatcg
     3421 cagctgctgt gggttcatta attaagcgta ccgcatttaa cccggctaac tccgctgcta
     3481 aacgggtatg cttgcgttgt tcatcgctga aataagccgg aacggagatc accacatctt
     3541 taatcggacg ttgcagaaat tcttcggcat cttcttttaa tgagcgtaat accaaagagg
     3601 acagttctgg cgcgttaaat gtgtcgctgc ctaaccgcca gttggtatta ctgcccattg
     3661 cacgtttaaa taacgctgcc gttttatccg gatgcgaagt acgccgtgat acagccggtt
     3721 ttccgactaa aatatgatta ttttcatcca tgctaattat ggatggtgtt aaatattcac
     3781 cgaacttatt tggaattaat tgcgcggcac cgtctttcca gacggcaatt aaactattgg
     3841 tagtgccgag atcgatacca atggcgagtt ctgcattatc cattgcattt atatcttgaa
     3901 agaaaaagag ataaacagat aaacagataa acagataaac agataaacag attaagacca
     3961 cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa tttcagacga
     4021 tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt ggcattcggt
     4081 ttattgccag tcagataata ataatcaaca accgtcatac cctggctata tttcccctgt
     4141 gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa cagccaggcg
     4201 atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc tttatgatat
     4261 tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc aatcgcgcgg
     4321 aaacgctcgg tgtcttcaac gtggatttgt gctttgtgag taacatccag accggccatc
     4381 accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac gtaaatatta
     4441 aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat cacgatacgg
     4501 gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt agtttgcggt
     4561 ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc catcagctct
     4621 accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc atccagaccg
     4681 ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt taccgcgccg
     4741 cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac attgcgtaag
     4801 gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc aagctctggt
     4861 gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca atctaacaga
     4921 attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg taacgccagg
     4981 atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact tgggtatgcg
     5041 cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc cgatttcgga
     5101 tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc cataaagatc
     5161 acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat catggtcatt
     5221 ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc cggatccagc
     5281 gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaatgc gcgagcgatt
     5341 gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc atgtgcggaa
     5401 agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt atcgcgttta
     5461 agcactttca cctgcgccag ggtcaggttt tcgataatcg acagatgagg gaacagctcg
     5521 aaatgctgga ataccatccc gacgcgggaa cgcagctttg ccagatcggt tttcttgtca
     5581 ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag gccgttgacg
     5641 gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac cacttcgcct
     5701 tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca ttttgaaaca
     5761 tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac aacgacgcgc
     5821 taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc tgcgtaccat
     5881 cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac acatacacca
     5941 gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc atcgcgcgga
     6001 acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc aacgccagcg
     6061 cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc tctgaatagt
     6121 aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata tcattttttg
     6181 gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac cacagcaaaa
     6241 ccataactaa aggaatcgag cggaatacgt taacgtaggc tttggcaaac cacgctacgg
     6301 gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata ccaatcacga
     6361 ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat ggcagggaag
     6421 ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc caggcaggcg
     6481 aactttacgt tcaaccagcg tcattaccag catgataacc gcgttaatca acacatacgc
     6541 cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca atttacccgc
     6601 ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt tcaccaggtt
     6661 catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca gcagtacgta
     6721 tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt gcccacgcgg
     6781 caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa acagccccag
     6841 gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct taaaccacat
     6901 gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga attgcacaat
     6961 caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga gaaaacggtt
     7021 tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa tccaggcgca
     7081 gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat aggtggtgtt
     7141 gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca taaatctact
     7201 ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac ccagcgtatt
     7261 gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg tgctacgtaa
     7321 caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt gccttgtcat
     7381 tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg ttcaggtttt
     7441 tcggcggaat gggatttttg aaccatttat caaaccattt ttccgcttca ccggaggtct
     7501 gcacctgcgc ggtggtgtca tccatcagct ttttgaactg cggatcgtct ttacgcaaca
     7561 tgcaaccgta ggcttcctga gactgcggct tgccgacgat ttcccagttg tctggtttct
     7621 tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca acggcacgac
     7681 cgctttccag ggtgcggaaa gagtcactgt gatctttggc gctgatgatg cgcatattca
     7741 ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg gaagtgacga
     7801 ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt ttggtcagca
     7861 ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg cgttcgacgt
     7921 tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt ggaatacggt
     7981 tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt ttctttttca
     8041 ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt tgctgattgt
     8101 cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca ccgtttttgg
     8161 cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt gccagtcctg
     8221 cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata tccaactcct
     8281 ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt gttatcgtct
     8341 gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct aaatgtaaag
     8401 atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga agcaaaattc
     8461 ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg gttgcgtcgc
     8521 ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa atacttaggg
     8581 gcgaattaat tacgattgta attttaggct gaaaaagtca tatttcggac agaattatct
     8641 tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc tttttacctg
     8701 tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta aatgttcaat
     8761 tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt tatctcgcac
     8821 aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc gcaaacgttg
     8881 cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt ctgcacagta
     8941 gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc gtggcggaac
     9001 agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc tttggtgggc
     9061 tcatcagcgc ctgttatgcg ttacgcgaaa gtgagcagat agtcatcagc gttggggcat
     9121 ccggggcgat tctgggaata gctggtgcag cgatagcaac acagtttgct tcaggtacgg
     9181 gcacatacca taaaaaccag cggcgagtat ttcctctgtt gggtatggtg gcgctgacac
     9241 tgttgtacgg tgcccggcaa acaggaatag ataacgcttg ccacattggc ggcctgattg
     9301 cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga ctcgttacgg
     9361 aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg cttgcgcagc
     9421 agcagatgga tgagtcagtt ttacaggtca ggcaaagcct gcgtgaagcg ttttatccac
     9481 aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa cgcaacgccc
     9541 tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat ttgctcgctg
     9601 agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg tatgccgcaa
     9661 ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc ctgcatacct
     9721 ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt aaagatcagg
     9781 gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact tcatttgaag
     9841 cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg attaccaccg
     9901 gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg gtgatgaatc
     9961 gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg gcggatatcc
    10021 cgctggagaa atacgacggg acggggacga gcggtaaacc aggcgcctgg gttatggcac
    10081 tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac atcgtgcgga
    10141 tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt ggaacgatat
    10201 cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgccgatagt caggggattt
    10261 cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt agcaggagcg
    10321 tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg tcatatggcc
    10381 tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc gtttatcctg
    10441 ccagctacgt taatcacctt cgtttttcag cggatggtag aaaaatattt gtcatggcga
    10501 agaacgggtt gatccaaatg gacaggacgc gctcgcttga tccgcaggca attattcgtc
    10561 atccccaata tggcaatgtg gcttgtatcc ctgaaccgta agaaaaaggg gcatgataac
    10621 catgccccaa agctcaatga gaaagtacgt tttgctcatg caggatacat caggcacatc
    10681 aacgtttacg tgctcgcaga ctcatcaaca cagcggcaaa accaaacagc gccgtcagca
    10741 cccacagtgg ccagttgccg gtacgtgcat acggtgtgag tccggtggtc ggtgtcacat
    10801 tagtggtcaa cacctcgcgg gtgaactgcg ggatcatcgc ctgaacctcc ccctgcgggc
    10861 caatcaccgc cgtaatgccg ttgttggtgc tgcgcaacag tgggcgcgcc agctccagcg
    10921 cacgcattcg cgccatctgg aagtgctgcc atggaccaat agacttacca aaccacgcat
    10981 cgttggagat agtcagcaga tagtcggtat ccgggcggaa gttatcgcgc acttgctcgc
    11041 cgagaatgat ctcgtagcaa atagccgcag taagctcaat accatttacc gacagcggcg
    11101 gctggatata tggcccacgg ctgaacgacg acatcggcag atcaaagaac ggtgctaacg
    11161 gacgcagaat cgactccagc gggacaaact cgccaaacgg cactagatgg tttttgttat
    11221 agcgatcggc tgattcgtag ctgtatggtg cacctttacc cagtgtgatg atggtgttgt
    11281 aggtatcgta gcggttctgc ttattgagac gcgcgtcgac aatcccggtt accagcgagc
    11341 tacctttatc gcgcaactca ccgtccagtg ctttgaggaa cggttgctga ttaatttcca
    11401 gatcggttat cgccgactcc ggccagataa tcaacgatga tttgcccatc agcggagccg
    11461 ttgcgttgta gtaaatcttc agcgtattaa gaagctgatc tccatcccat ttcagtgatt
    11521 gcggaatatc gccctgaacc atcgaaacct gaatggtttt ctccggttgc ggggtaaacc
    11581 attggatgta acgcagcggg aagggtaggg caaacagcac gacggccacc accagcggac
    11641 gccagttgcg tttgaccaac gccagtgcca gcaggccact aaccatcatc agcaggaagt
    11701 taatggcttc cacgcccatt atcggtgcca gcccttttaa cgggccatca atctggctat
    11761 agccgaactg taaccacggg aagcctgtca gtacccaacc gcgcagaaac tcggtcactt
    11821 gccagagagc aggggcggca atcgccacgc gcagccaggt ggttttcggc cacagacgcg
    11881 acagcacgcc agcaaacagt ccggtataca gcgacaaata cgccgccagc agcaccacca
    11941 ggaagatgtt aaccgggcca ggcattccgc caaaggtcgc gatgctgaca tagacccagt
    12001 taataccgct gccaaagagg ccaaatcccc agcaaaagcc aatagcggca gactggagtg
    12061 gacggcggtt aaaggtcaac gcctgaagcc ccatcaacga aataatcgcc gcaggccaga
    12121 cgtcgtaagg agagaaggcc agcgttccgc aggcaccgaa taataacgcc agcagcaggc
    12181 gaatgcgctg gcgttcaatt aatgaggcaa aagccatgta gttatctatc cagtttcggt
    12241 tttattcatc cagcttcggc tggggtgagt catccgggat tttgacatga acctgaataa
    12301 tacgccgact gtcggccatc gccactttga actggtaacc gtcgatgtcg atagtttcgc
    12361 cacgcgccgg aagatgccca aatgcctgca tcaccagacc accgatagtg tcaacctctt
    12421 catcgctaaa gtgggtgccg aacgcttcgt tgaagtcttc aatggaagcc agtgcacgca
    12481 cggtccaggt atgacgactc agctgacgga agtcgatatc atcttcttcg tcatactcgt
    12541 cttcaatctc accaacaatc agttccagga tgtcttcaat ggtcaccaga ccggaaaccc
    12601 caccgaattc gtcaatgacg atcgccatgt ggtaacgctg agagcgaaac tctttcagca
    12661 tccggtctac gcgcttactt tcaggaacga caaccgcctg acgtaacact ttgtccatgc
    12721 tgaaggcttc agcatcgctg cgcataaacg gcagcaagtc tttcgccatc agaatccctt
    12781 caatgtgatc tttgtcttcg ctaatcaccg ggaaacgtga gtgggcggac tcgatgatga
    12841 catcaaggca ttcgtccagc gtctggttgc gtttcagggt aatcatctgg gagcggggga
    12901 tcatgatgtc gcgaacgcgt tggtctgcga tgtccatcac cccttcgagc atatcgcgcg
    12961 tatcttcgtc gataaggtcg ttctgcccgg aatcacggat cagcgccagc agttcgtcac
    13021 ggtttttcgg ttcaccgtgg aaaagttggc tgagtaacag ggagaaaaat cccttcttgt
    13081 tgcttatcgt gtcactactg tgtgaattgt cgtcgctcat ggcgtcgtat gggttctcat
    13141 gttagttaat caaaacgccg tcgttaatca ccaacggcgg ggacgtctgc cagtcaaatg
    13201 cctggcaaat tattctttct cggcaatgta cggatcctca tagcccagag caagcataat
    13261 ctctgtttcg agggcttcca tttcttctgc ttcgtcatct tcgatgtgat cgtaacctaa
    13321 caaatgcaga ctgccgtgca ccaccatatg cgcccagtgc gcctccagtg gtttgccttg
    13381 ctcctgagct tccttctcaa ccacctgacg gcagataacc agatcgccca gtagcgacat
    13441 ttccatgcca ggcggcactt caaacgggaa ggagagcacg ttggtcggct tatccttacc
    13501 gcgataggtc agattcagac tgtggctttc ggcggtatcg accacgcgaa tagtcacttc
    13561 cgattcttcc tgaaactgcg ggatcaccgc attcagccat gtttgaaact ggctctcttc
    13621 cggtaacccg gaattatctt cacatgccag ttgtaaatcg aggatcacct gactcatttt
    13681 tgttcctgtt cttcgcgctt gcgttctgcc gccagcgccg cttttcgttt ttgttcggct
    13741 tcttcccagg cttcataggc gttaacgata cgcgccacca cagggtgacg aaccacgtct
    13801 tcgctgtgga agaagttaaa gctgatctct tcgacatcgg ccagcacttc gatggcgtga
    13861 cgtaagcctg atttagtatt acgcggcagg tcgatctgtg tgacgtcgcc ggtgataacc
    13921 gcttttgagt taaaaccgat acgggtcagg aacatcttca tctgttcgat ggtggtgttc
    13981 tggctctcat cgagaatgat aaacgcgtcg ttcagcgtac gaccacgcat ataggccagc
    14041 ggcgcgactt caataacgtt gcgctcaatc agtttctcaa ctttctcaaa gcccagcatt
    14101 tcaaacagcg cgtcgtacag tgggcgcaga tacgggtcta ctttctggct taaatcgcca
    14161 ggcaggaagc ccagtttctc accggcttcg accgccggac gagtcagcag aatacggcga
    14221 atctcctgac gctccagggc atcaactgcc gcagccactg ccaggtaggt tttacccgta
    14281 cccgccgggc caacgccgaa ggtaatgtca tggtcgagaa tattggcgat gtactgcgcc
    14341 tggtttggcg tgcgcggctt aattacgccg cgtttggttt tgatattgac cgctttgccg
    14401 tactccggca cgctctccgc actttgctcc agtactcgcg cttctttaat cgcaaggtgg
    14461 atctgttccg gttcgatatc ctgaatctga ccgcgcatcg gggctgtatc gacatacagg
    14521 ctacgcagaa tgtctgccgc agcggtgacg caaatcggac ggccggtcag tttaaagtgg
    14581 ttgtcgcggc gattgatctc gatgccgaga cggcgttcga gctgcttgat gttgtcatca
    14641 aacgggccgc acaggctcaa cagacgcgcg ttgtctgctg gctccagggt gatttcgcga
    14701 gtgtctatgt tcaaaccgtt cctcttatct gtatgccgcc ggaagctgaa cattcaccgg
    14761 cctataagga aattattcac gccacaggaa aaaggcgcaa gcattgcgat aaagatgggg
    14821 ataaagagag aaaaaacaag gcccaccgga acggcgggcc tgagaattac ggctgataat
    14881 aacccacgcc aaggtcgttt tctttgcggg tacgggcaat caccgattcc ggtgtttctg
    14941 ccacgcgcag acccatttca tcttcagtac gcaccacttt accgcgcaga gagttcgggt
    15001 agacgtcggt aatttctaca tcgacgaatt taccgatcat atccggtgtg ccctcgaagt
    15061 tgaccacgcg gttattttcg gtacgcccgg aaagctccat gatgctctta cgcgaagtcc
    15121 cttctaccag aatacgctgg gtggtgccga gcatccggcg gctccatgcc atcgcttgct
    15181 gattgatacg ttcttgcaga atatacagac gttgcttctt ctcttcttcc ggaacatcat
    15241 caaccatatc ggcggctggt gtacccggac gtgcagagaa gataaagctg tagctcatgt
    15301 cgaaattgac gtcggcaatc agcttcatcg ttttctcgaa gtcttcggtg gtttcgccag
    15361 ggaagccaac aatgaagtca gaactgatct gaatatccgg acgcgccgca cgcagtttac
    15421 ggatgatcgc tttgtactcc agcgccgtgt gagtacggcc catcaggttc agaatgcgat
    15481 cggaaccgct ttgtaccggc agatgcagga agctcaccag ctccggcgtg tcgcgataca
    15541 cttcgatgat atcgtcggtg aattcgatcg gatggctggt ggtaaagcga atacgatcga
    15601 tcccgtcgat cgcagcaacc agacgcagca gatcggcaaa cgatccggtg gagccgtcgt
    15661 agttttcacc acgccaggcg ttcacgttct gaccgagcag gttgacttca cgcacgccct
    15721 gagccgcaag ctgggcaatc tcaaacagaa tatcgtcaga cggacggctt acctcttcac
    15781 cacgggtgta aggcaccacg cagtaggtgc aatatttatt gcagccttcc atgatggaga
    15841 caaacgcggt cggcccttcg gcgcgcggtt ccggtagacg gtcaaacttc tcgatttccg
    15901 ggaagctgat atctacaacc gggctgcggt cgccgcgcac ggagttgatc atctccggca
    15961 gacggtgcag cgtttgcggc ccaaaaataa tatcgacata gtgggcgcgc tggcgaatgt
    16021 gctcgccttc ttgcgatgcc acgcagccac cgacgccgat aatcaggtct ggattcttct
    16081 cttttaacag tttccagcga cccaactgat ggaagacttt ttcctgagcc ttctcgcgga
    16141 ttgagcaggt gttcagcagc agcacatccg cttcttccgc cacgtcggtc agttgatagc
    16201 cgtgggtggc atccagcaga tcggccatct tcgatgaatc gtactcgttc atctgacagc
    16261 cccaggtttt aatatggagt tttttggtca tcgacttgct cttgcgaaat agtagccagg
    16321 aatgcagggc gtatagtgta atgctttgct gccgttgtga ccagtatgag cgttatcagc
    16381 ccttgggggt aaaaatcctg taaacttaaa gcatattgct aacaggatga ttgaccatga
    16441 caaatcaacc aacggaaatt gccattgtcg gcggaggaat ggtcggcggc gcgctggcgc
    16501 tggggctggc acagcacgga ttttcggtaa cggtgatcga gcacgcagaa cctgcaccgt
    16561 ttgtcgctga tagccaaccg gacgtgcgga tctcggcgat cagcgccgca tcggtatcat
    16621 tgcttaaagg gttaggggtc tgggatgcag tacaggctat gcgttgccat ccttaccgca
    16681 gactggaaac gtgggagtgg gaaacggcgc atgtggtgtt tgacgccgct gaacttaagc
    16741 taccgttgct tggctatatg gtggaaaaca ccgtcctgca acaggcgctg tggcaggcgc
    16801 tggaagcgca tccgaaagta acgttacgtg tgccaggctc gctgattgca ttacatcgag
    16861 ataatgatct tcaggagctg gaactgaagg gcggtgaaac gatccgcgcg aaactggtga
    16921 ttggtgccga cggcgcaaat tctcaggtgc ggcagatggc gggaattggc gttcatgcct
    16981 ggcagtatgc gcagtcgtgc atgttgatta gcgtacagtg tgagaacgat cccggcgaca
    17041 gcacctggca gcaatttacc ccggacggac cgcgtgcgtt tctgccgttg tttgataact
    17101 gggcatcgct ggtgtggtat gactctccgg cgcgtattcg ccagttgcag aatatgaata
    17161 tggcgcaatt ccaggcggaa atcgcgaagc atttcccgtc gcgtctgggt tacgttacac
    17221 cgcttgccgc tggtgcgttt ccgctgacgc gccgacatgc gttgcagtat gtgcagccgg
    17281 ggcttgcgct ggtgggcgat gccgcgcata ccatccatcc gctggcgggg cagggggtga
    17341 atcttggtta tcgtgatgtc gatgccctga ttgatgttct ggtgaatgcc cgcagctacg
    17401 gtgaagcgtg ggccagttat cctgtcctca agcgttacca gatgcggcgc atggcggata
    17461 acttcattat gcaaagtggt atggatctgt tttatgccgg attcagtaat aatctgccgc
    17521 cgctgcgttt tgtgcgtaat ctcgggttaa tggcggcaga gcgtgctggc gtgttgaaac
    17581 gtcaggcgct gaaatatgcg ttagggttgt agccttacaa cattgccgga tgcgtgccaa
    17641 ccgtaggtcg gataagacgc ggcagcgtcg catccgacat tgaaggataa gacgtgtcaa
    17701 catcgcattc gacattgaat gaacgcagaa aagcaaaaaa ctcgccgaag cgagcttttt
    17761 taatgtggct ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct
    17821 taccgcttgg cgatacccca actggtgcac ttacgatgta agcgtcttga ataaattggc
    17881 tggggtacga ggattcgaac ctcggaatgc cggaatcaga atccggtgcc ttaccgcttg
    17941 gcgatacccc aacaaattgg tttttgaatt tgccgaacgt attcgacaca ttcagaattt
    18001 ggtggctacg acgggattcg aacctgtgac cccatcatta tgagtgatgt gctctaacca
    18061 actgagctac gtagccagat tgtttcttcg atggctgggg tacctggatt cgaaccaggg
    18121 aatgccggta tcaaaaaccg gtgccttacc gcttggcgat accccaataa ccggtcggtg
    18181 aaccgcttac tcgaagaaga tggctggggt acctggattc gaaccaggga atgccggtat
    18241 caaaaaccgg tgccttaccg cttggcgata ccccatccgt acaacgcttt ctggtgaatg
    18301 gtgcgggagg cgagacttga actcgcacac cttgcggcgc cagaacctaa atctggtgcg
    18361 tctaccaatt tcgccactcc cgcaaaaaag atggtggcta cgacgggatt cgaacctgtg
    18421 accccatcat tatgagtgat gtgctctaac caactgagct acgtagccat ctttttttcg
    18481 cgatacctta tcggcgttgc ggggcgcatt atgcgtatag agccttgcag cgtcaacctc
    18541 tttttcaagg aaaattgctc gaaagtgact gtttggttag gttgcgaaca gcgtggcgct
    18601 atattcgtca attattgttt actttgtgtt ttttccccac cctacagcca ttcttttgtc
    18661 atacaggatg aaattcggaa ttcaacaatg gtggtgggaa attaatctat gaaatactgg
    18721 cctacagtga tgagttgtca aacagtgatg tggcaaaccc ggaacatttc cttactgcat
    18781 atcagaatca acaagcacct caataactga aacagccccg gatttcaccg gggctgtttc
    18841 gcatttctta cttatacgcc gactggtgaa cgccaaccgc acgaccagac ggatcgtcca
    18901 ttttcttgaa cgcctcatcc cactcaatcg ctttagcgga agaacacgcg acggacgggc
    18961 cgcccggcac gcactcagcg gcgctcggaa gcgggaacag ttcttcaaag atctcccggt
    19021 acagatacgc ttcttttgag gttggcgtgt tgtacgggaa gcggaagcgg gcagtttcca
    19081 gttgctgatc agaaacctgc tgcgccgcca cttctttcag ggtgtcgatc caactgtaac
    19141 cgacgccatc ggagaactgc tctttctgcc gccaggccac gcttgcgggc aggtatgact
    19201 caaaacattc acgcaggatg tgtttttcca ttttgccgtt accgcacatt ttatcctgcg
    19261 ggttaatgcg catcgccaca tcaaggaatt ttttgtcgag gaacggaacg cgtgcttcca
    19321 cgccccaggc tgacatcgct ttgttggcgc gcgcgcagtc atacatatgc agggccagca
    19381 gtttacgcac cgtctcttca tgcagttctt tagcgttggg cgctttatgg aagtaaagat
    19441 aaccgccaaa cacttcatca gaaccttcac cggacagcac cattttaatg cccatcgcct
    19501 tgatcttacg cgacattaaa tacatcggtg ttgaagcgcg aatagtggtc acatcataag
    19561 tttcgatgtg gtaaatcacg tcgcggatgg catccagacc ttcctgtaca gtgaagtgaa
    19621 tttcgtgatg caccgtgccc agatggtttg ccacttcctg ggctgcttta agatccggtg
    19681 aacccggcag acctacagca aaggagtgta actggggcca ccaggcttca gagcgttcct
    19741 gatcttccac gcgacgggct gcgtatttct tggtgatagc ggaaataatt gaggaatcca
    19801 gaccaccaga aagcagcaca ccgtaaggca catcagacat cagatggctt ttaacggaat
    19861 cttccagagc ctgacgcagc tcgtttttgt cggtcacgtt atctttcact gcatcaaagt
    19921 cgaaccagtc gcgatgataa taagaacgga tttcgccgtc ctggctccac aaatagctcc
    19981 ccgccgggaa ctctttaatc gtgcggcaaa ctggcaccag cgatttcatt tctgaggcca
    20041 catacagctg accgtgttcg tcatacccca tatacagtgg gatgatcccc agatggtcgc
    20101 gaccaatcag gtaggcatct ttttcgctgt cgtacagggc aaaggcaaac atgccctgca
    20161 agtcgtcgag aaattccggc cctttttcct gatacagcgc gaggatcact tcacagtcag
    20221 accctgtctg gaactgataa cgatcgccat attcagcgcg caatgcctgg tggttgtaga
    20281 tttcaccgtt taccgccagt acgtgggttt tttgttggtt gtagagaggt tgcgcccccg
    20341 cgttaacgtc aacaattgac agacgttcgt gagcgagaat ggcgttatcg ctggcataaa
    20401 taccggacca gtccgggcca cgatgacgca tcaggcgtga cagctcgagt gctttcttac
    20461 gctgctcaac tgcgtctgtt ttgatatcga atacgccaaa aattgaacac ataaccttct
    20521 ccgttaacct ggtatttgtt gcttgttgtg tttgcttgtt taaaaaaatg ccgcaaagca
    20581 gcactgtgcg caagcgattt ggcggtgaaa aaataaaaaa cgtaatggtg attgtcgatt
    20641 ggtgaaaaag gggctggtgt gaggatgtat ttattgatga atcgataatt tttagcgggt
    20701 tttattgaat ggttatattt tacgggggcc
//
LOCUS       DAZPLM010000062        20661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPLM010000062 DAZPLM010000000
VERSION     DAZPLM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.062"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(6..1655)
                     /gene="actP"
                     /locus_tag="ACHVC7_001906"
     CDS             complement(6..1655)
                     /gene="actP"
                     /locus_tag="ACHVC7_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR2115971.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1652..1965)
                     /locus_tag="ACHVC7_001907"
                     /pseudo
     CDS             complement(1652..1965)
                     /locus_tag="ACHVC7_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2165..4123)
                     /gene="acs"
                     /locus_tag="ACHVC7_001908"
     CDS             complement(2165..4123)
                     /gene="acs"
                     /locus_tag="ACHVC7_001908"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR2115972.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4517..5953
                     /gene="nrfA"
                     /locus_tag="ACHVC7_001909"
     CDS             4517..5953
                     /gene="nrfA"
                     /locus_tag="ACHVC7_001909"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR2115973.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5998..6564
                     /gene="nrfB"
                     /locus_tag="ACHVC7_001910"
     CDS             5998..6564
                     /gene="nrfB"
                     /locus_tag="ACHVC7_001910"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR2115974.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6561..7232
                     /gene="nrfC"
                     /locus_tag="ACHVC7_001911"
     CDS             6561..7232
                     /gene="nrfC"
                     /locus_tag="ACHVC7_001911"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR2115975.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7229..8185
                     /gene="nrfD"
                     /locus_tag="ACHVC7_001912"
     CDS             7229..8185
                     /gene="nrfD"
                     /locus_tag="ACHVC7_001912"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR2115976.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8205..9923
                     /locus_tag="ACHVC7_001913"
     CDS             8205..9923
                     /locus_tag="ACHVC7_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR2115977.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9916..10299
                     /gene="nrfF"
                     /locus_tag="ACHVC7_001914"
     CDS             9916..10299
                     /gene="nrfF"
                     /locus_tag="ACHVC7_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR2115978.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10296..10892
                     /gene="nrfG"
                     /locus_tag="ACHVC7_001915"
     CDS             10296..10892
                     /gene="nrfG"
                     /locus_tag="ACHVC7_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR2115979.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11234..12547
                     /gene="gltP"
                     /locus_tag="ACHVC7_001916"
     CDS             11234..12547
                     /gene="gltP"
                     /locus_tag="ACHVC7_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR2115980.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12626..13315)
                     /gene="yjcO"
                     /locus_tag="ACHVC7_001917"
     CDS             complement(12626..13315)
                     /gene="yjcO"
                     /locus_tag="ACHVC7_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR2115981.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="ACHVC7_001918"
     CDS             complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="ACHVC7_001918"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15137..15139),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR2115982.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="ACHVC7_001919"
                     /pseudo
     CDS             complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="ACHVC7_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="ACHVC7_001920"
     CDS             complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="ACHVC7_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR2115983.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="ACHVC7_001921"
     CDS             complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="ACHVC7_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR2115984.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20318..20593)
                     /locus_tag="ACHVC7_001922"
     CDS             complement(20318..20593)
                     /locus_tag="ACHVC7_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR2115985.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4645 a         5494 c         5659 g         4863 t
ORIGIN      
        1 agagattaat gtgcgcggcc ttgctcaacg ccaaagccgg tctgggagcg gataaactgc
       61 gcgcggaaca gttcacgctc gcgcgcccct tccgctgagt tatcggttgc cgagaagaac
      121 cagatgccga ggaatgccac ggtgatagag aacagcgccg ggtattcata cgggaagatg
      181 gctttttcgt gaccaaggat ctgtacccaa atcgtcgggc cgaggatcat cagtactact
      241 gcggtaatca gccccagcca gccacccatc atcgcgccac gcgtggtcag tttcgaccag
      301 tacatagaaa gcagaatgat cgggaagtta cagctcgccg cgatggcaaa cgccagcccc
      361 accataaagg cgatgttctg attctcaaac agcacgccga ggataatcgc aatcacgccg
      421 aggatcagta cggtgatttt tgatacccgc agctcttcac gttcggtcgc gccttttttg
      481 aagacgttag cgtacaagtc atgcgaaacc gcggatgcgc ccgccagcgt cagacccgca
      541 accaccgcga ggatagtggc gaaagcaacc gctgaaataa aaccgaggaa caggttgccg
      601 cccactgcat tcgccaggtg aacggccgcc atgttgttac caccaatcag atggcccgcc
      661 gcgtctttat attccggatt cgcaccaacc agcatgatcg cgccgaagcc gataataaag
      721 gtcagaatat agaagtagcc cataaacccg gtggcgtaga acacgctctt acgtgcttcg
      781 cgggcatcgc tgactgtaaa gaagcgcatc agaatgtgcg gcaagcccgc cgtaccaaac
      841 atcagtccca gacccagaga gagtgcggag atcggatctt tcaccagccc gcccggcttc
      901 atgatgtcga cacctttcgg gtgtaccgcc atcgcttcac tgaacagatt gttgaagcta
      961 aagccgacgt gtttcatcac cataaaggcc ataaagctgg caccgaacag caacagcacg
     1021 gctttgataa tctgtaccca agtggtcgcc agcatgccgc cgaacaggac gtacatcatc
     1081 atcagcacgc cgaccagcac caccgcaatg tgatagttaa ggccaaacag cagctcgatc
     1141 agtttacctg cgcccaccat ctgggcgata aggtaaagcg ccaccaccac cagagaacca
     1201 caggccgaaa gaatacgaat cggcccttgt ttcagacggt aagaggccac atcggcaaag
     1261 gtgtagcgcc ccaggttacg cagacgttcg gcaatcagga acaaaatgat cggccagccc
     1321 accaggaagc ccagcgagta aattaagccg tcatagccgg aggtaaacac cagcgcggag
     1381 atccccaaga atgaggcggc ggacatatag tccccggcaa tcgccagccc gttctggaag
     1441 ccagtgatat tgccgcctgc ggtgtagtag tcgctacgag aacgtacgag ttttgatgcc
     1501 cagtaggtaa tgccgagcgt aaacacgacg aaaatcagga acataataat cgcctgccag
     1561 ttcgttggct ggcgctctac ggccccgcta atagcatccg cggcgttagc tgcgaaaggg
     1621 agtgtggcgg caagcgccgt cagaactctt ttcatgatgc ttgtacctca tgcaggactt
     1681 cattattaag acggtcgaat tcgccgttcg cccgccagat gtagataccg gtgagaacaa
     1741 aggagatcac aatcactcca acaccaattg gaataccgcg tgtgacgctg gtgttcggat
     1801 tcagcggcgt gccagccagc cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac
     1861 tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga ctaactccct
     1921 gaaatgcgca ttgtcttcta tccgctgata aatagtgcca ttcatcacag attctccaga
     1981 ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa cgctcgggta
     2041 acccttgccg aatgtaggcc ggataaggcg tttacgccgc atccggcaat caatgcctga
     2101 tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac cgactcgcat ccggcaattg
     2161 tgggttacga tggcatcgcg atagcctgct tctcttcaag cagcttctcg actacgccag
     2221 gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc gcccgccgca attttgcgca
     2281 gaatacggcg cataattttg ccggagcggg ttttaggcag ggagtcggtc cagtgcagca
     2341 cgtctggcgt cgccagcggg ccaatctctt tacgcaccca gttgcggact tctgcgtaca
     2401 gttctggtga cggttcctcc ccgtgattaa gcgtgacgta ggcgtagatc gcctgacctt
     2461 taatattgtg cggaatacct actacggcgg cttcggcaat cttcggatgc gccaccagcg
     2521 ccgactcaat ctctgccgtc cccagacggt gaccggagac gttcagcacg tcgtccacac
     2581 gcccggtgat ccagtaatag ccatcttcat cgcgacgcgc gccgtcgccg ctgaaataca
     2641 tatttttgaa ggtggagaag taggtctgtt caaaacgttc gtgatcgcca aacagcgtac
     2701 gcgcctgacc cggccaggag tcggtgatca ccaggctgcc ttcggtagcg ccttccagcg
     2761 ggttaccttc gttatcgacc agcgccggtt gcacgccgaa gaacggacgt gttgccgaac
     2821 cggctttcag ctcggtagcg ccaggcagcg gcgtgatcat gaaaccgcca gtttcggtct
     2881 gccaccaggt atcgaccacc ggacatttct cgttgccgat ttttttccag taccactccc
     2941 acgcttccgg gttaattggc tcgcccacgg aaccgagaat gcgcagcgac gaacggtcgg
     3001 taccctcgat cgctttatcg ccttccgcca tcagcgcgcg gatcgccgtg ggtgcggtat
     3061 agagaatatt gacctgatgc ttgtccacca cctgcgccat acgggcaggc gtcggccagt
     3121 tgggtacgcc ttcaaacatc agcgtggtcg caccgcaggt cagcgggccg tacagcaagt
     3181 aactgtgtcc ggtcacccag cccacatcgg cggtgcacca gtagatatca cccggatgat
     3241 aatcaaagac atatttaaag gtcagcgccg cgtacaccag ataaccgccg gtagtatgca
     3301 gcacaccttt tggcttaccg gtagaaccgg aggtgtagag aataaacagc ggatcttcgg
     3361 cgttcatctt ttccgcctgg tgctgatcgc tcgcttgctc aaccaggtcg tgccaccaca
     3421 ggtcgcgccc ttcctgccag tcaattttcc cgccagtacg cttcagtacc accacatgct
     3481 ctacgctggt gacgttcggg tttttcagcg cgtcatcaac gtttttcttc agcggaatac
     3541 tgcgcccggc acgcacacct tcgtcggaag tgatcaccag tcgtgagttg gaatcaataa
     3601 tgcgcccggc aacggcttcc ggcgagaagc cgccgaaaat caccgaatgc accgcgccaa
     3661 tgcgggcgca ggccagcatc gcaaccgcgg cttccggcac catcggcata taaatcgcca
     3721 ccacatcacc ttttttaatg cccagctcga gcagggtatt ggcgaagcgg cagacgtcgc
     3781 ggtgcagctc tttatagctg atatgtttgc tctggctggc gtcgtcgcct tcccagatga
     3841 tggcggtacg atcgccgttt tcttgcagat ggcggtcaag gcagtttgcc gccagattca
     3901 gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg ggcaaaggag gtgtttttca
     3961 ccttctggta aggtttgatc cagtcaagaa tttttccctg ttcgccccag aaggtatcag
     4021 gtacgttaat agattgttga tacatcgcct cgtactgctg agggtttatc aggcaacggt
     4081 ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttggct catgcttttg ttctccttgt
     4141 agggtgttaa taatatgtgg cataagcgtt aaatgtaggg gtattggcag ttttgtttag
     4201 tatttgggcg acagatcacg caaaagtaga attgtgcaaa taaacggcag ggtaattttt
     4261 gaaggtcagc aacaaaagtt gattaattct ttgaggaaca tgcagttatg cattgctgtt
     4321 gaaaaagagg aagatactaa ctaactctaa agtggtattt tacatgcact tacaattgat
     4381 taaagacaac attcacagtg tggttatttg ttacacatag gggcgagcaa tgtcatgaca
     4441 gtgtaggtgc ggttactgtc gtgaaaaaca ataaaaaccg ccattgtaac aatggcgcaa
     4501 ttcggatgaa gcccctatga caaggataaa aataaacgca cgccgtatct tcagcttatt
     4561 gattcctttt ttctttttca cttctgttca cgctgaacaa acggcagctc ccgcaaaacc
     4621 tgtaactgtg gaagcgaaga atgaaacctt tgccccgcag catcccgatc aatatctctc
     4681 ctggaaagcc acctcggaac agtcagagcg tgttgacgcc ctggcggaag atccacggct
     4741 ggtgatcctg tgggcggggt atcccttctc gcgcgattac aacaagccgc gtggacatgc
     4801 ctttgctgtg accgatgtgc gtgaaaccct gcgtaccggt gcgccgaaaa acgctgaaga
     4861 tggtccgcta ccgatggcgt gctggagttg taaaagcccg gatgtggcgc gtctgatcca
     4921 gaaagacggc gaagatggct acttccacgg taagtgggcg cgcggcggcc cggaaatcgt
     4981 caacaactta ggttgtgccg actgccataa caccgcctca ccagagttcg ccaaaggcaa
     5041 accggagtta accctttccc gtccgtatgc ggctcgcgcg atggaagcca ttggtaaacc
     5101 ttttgagaaa gccggacgtt tcgaccagca atcgatggtt tgcggtcagt gccatgtgga
     5161 gtattacttc gacggcaaaa acaaagcggt taaattcccg tgggatgacg gcatgaaagt
     5221 cgaaaatatg gagcagtatt acgacaaaat tgccttctct gactggacta actccctgtc
     5281 gaaaacgcca atgctgaaag cgcagcaccc ggaatatgaa acctggacag cgggcattca
     5341 cggtaaaaac aacgtgacct gtatcgactg ccatatgcca aaagtgcaga acgccgaagg
     5401 caaactctat accgaccata aaattggtaa tccgtttgat aacttcgccc agacttgtgc
     5461 gaactgccat acccaggaca aagctgcctt gcaaaaagtg gtcgcggaac gtaagcagtc
     5521 gattaacgac ctgaaaatca aggttgaaga tcaactggtt cacgctcact tcgaagcgaa
     5581 agcagcgctg gatgcaggcg cgacggaagc tgaaatgaag ccaattcagg acgatatccg
     5641 tcatgcccag tggcgctggg atctggcgat cgcttcccac ggcattcata tgcacgcacc
     5701 ggaagaaggt ttacggatgc tcggtacggc gatggataaa gcggcggatg cacgcaccaa
     5761 actggcgcgc ctgctggcga ccaaaggcat cacccatgaa atccagatcc cggatatctc
     5821 aaccaaagag aaagcccagc aggccattgg cctgaacatg gaacaaatca aggccgagaa
     5881 gcaggacttc atcaaaacgg tgatcccgca gtgggaagaa caggcacgta aaaacggtct
     5941 gttaagccaa taaccccgtt ccgcctcgca aggggcggaa aacacaatgg agtgaatatg
     6001 agcgtattac gttcgttatt aactgccggg gtgctggcgt cgggcctgtt gtggagcctg
     6061 aacggaatta ccgctacccc tgcggcgcag gcatctgatg atcgctacga agttacccag
     6121 cagcgtaacc cggatgccgc ctgtctggac tgtcataaac cagataccga aggtatgcat
     6181 ggcaaacatg cttccgttat caacccgaat aacaaactgc cggtcacctg caccaactgt
     6241 cacggccagc catcaccgca acaccgcgaa ggggtgaaag atgtgatgcg ctttaacgag
     6301 ccgatgtaca aggttggaga gcagaacagc gtctgtatgt cctgtcatct gccggaacag
     6361 ttgcaaaaag cgttctggcc gcacgatgtc cacgtaacca aagtggcgtg cgccagctgc
     6421 cattccctgc atccacagca agatacgatg cagacgttaa gtgacaaagg acggattaag
     6481 atttgcgtcg attgccacag cgatcagcgc accaatccga actttaaccc ggcgtctgtt
     6541 ccgttgctta aggagcagcc atgacctggt ctcgtcgcca gtttctcacc ggcgtcggcg
     6601 tgctggcagc cgtcagcggc accgcagggc gcgtggtggc gaaaacgttg aatatcaatg
     6661 gggtgcgtta cggcatggtg catgacgagt cgttatgcat cggctgtacc gcctgtatgg
     6721 atgcttgtcg ggaagtgaac aaggtgccgg aaggcgtctc gcgcctgacg attattcgta
     6781 gcgagccgca gggcgaattt cctgatgtga aatatcgctt cttccgtaag tcttgccagc
     6841 actgcgatca tgcgccgtgc gttgacgtct gcccgaccgg tgcgtctttt cgtgatgccg
     6901 ctagcgggat tgtcgatgtt aatcctgatc tctgcgtcgg ttgtcagtac tgcatcgccg
     6961 cctgtccgta ccgcgtgcgc tttatccatc cggtcacgaa gacggcggac aaatgcgatt
     7021 tctgccgtaa aaccaatttg caggccggta agctgcccgc gtgtgttgag gcttgcccga
     7081 cgaaggcgct gacgtttggc aatctggacg atcctaacag tgagatttcg caactgctgc
     7141 gccagaagcc tacttaccgc tacaagctgg cgctgggaac caaaccgaag ctgtaccgcg
     7201 taccgtttaa atacggggag gtgagccaat gacgcagact tccgcatttc attttgaatc
     7261 gctggtgtgg gactggccga ttgccatcta cctgtttttg attggtattt ctgccggtct
     7321 ggtgacgctg gccgtgctgt tacgtcgctt ctacccgcag gcgggcggtg cagacagtac
     7381 gttgctgcgc accacgctga ttgtcgggcc gggcgcggtg atcctcggtc tgttgatcct
     7441 cgtcttccac ctgacaagac cgtggacctt ctggaagctg atgttccact acagttttac
     7501 ctcggtgatg tcgatggggg tgatgctgtt tcagctctac atggtggtgc tggtgctgtg
     7561 gctggcgaaa atctttgaac atgatttgct tgccctgcaa caacgctggt tgccgaagct
     7621 ggggatcgtg caaaaggttc tgagcctgct gacgcccgtt catcgcggac tggaaacgct
     7681 gatgctggtg ttggcggtgc tgctgggggc ttataccggc tttctgctgt cggcgctgaa
     7741 atcgtatccg ttcctcaata acccgatcct gccggtgctg ttcctcttct ccggcatctc
     7801 gtccggtgcg gcggtggcgc tgatcgccat ggcgatacgc caacgcagta acccgcattc
     7861 cacggaagcg cactttgttc accggatgga aatccccgtg gtatggggtg aaatcttcct
     7921 gctggtggcg ttttttgtcg gtctggcgct gggcgatgac ggtaaagttc gtgcgctggt
     7981 ggcggcatta ggtggcggtt tctggacgtg gtggttctgg cttggtgtcg ccgggctggg
     8041 gctgattgtg ccaatgttgc tcaaaccgtg ggtcaatcgc agttccggca ttcctgccgt
     8101 gctggcggcg tgtggggcca gtctggtcgg cgtgttgatg ctgcgctttt tcattctcta
     8161 cgccggacag ttgacggtgg cgtaagccag aaaagaggtg gtttctggac gtattccttc
     8221 ctgaagtcgg ttttctggcg ttgttgttaa gtctcggggt caacgtgttg accccgttga
     8281 cggcctttgc gggagtgcgg ttgcgctggc ctgccatgat gcgactcact tgcatcggca
     8341 ttctggcgca gttcgcgctc ctgctgctcg cctttggcgt actgacgtat tgttttctca
     8401 tcagcgattt ctcggtcatt tatgtcgcgc aacatagcta tagcctgctg tcgtgggaat
     8461 tcaagctggc agcggtgtgg ggcggccatg aaggttcgct gctgctttgg gtgctgctgc
     8521 tttccgcctg gagcgcgctg tttgcctggc attatcggca gcaaaccgat ccgctatttc
     8581 cgctgacgtt agccgtttta tctctcatgc tcgccgcact gctactgttt gtggtgctgt
     8641 ggtccgatcc cttcgtgcgg atatttccac cagcaatcga aggccgcgat ctcaatccga
     8701 tgctgcaaca tcccggtctt atctttcatc caccgctgct ttaccttggc tatggcggtt
     8761 tgatggtagc ggcgagcgtg gcgctggcga gtctactgcg cggcgagttt gatgcggcca
     8821 gcgcccgaat ttgctggcgc tgggcgttac ctggctggag tgcattaacg gcggggatca
     8881 tcctcggttc ctggtgggcc tactgcgaac tcggctgggg cggctggtgg ttctgggatc
     8941 cggtggaaaa tgcctcttta ttaccctggc tttctgccac tgcgctgctg cacagtttat
     9001 ccctgacacg ccagcggggg attttttgcc actggtcgct gttgctggcg atagttactc
     9061 tgatgctgtc gctgctgggc accttaattg tccgttctgg cattctggtt tcggttcatg
     9121 cgttcgcact ggataacgtc cgcgccgtgc cgttgttcag cctgtttgca ctgattagcc
     9181 ttgcgtcttt ggctctgtat ggctggcgag cgcgggacgg tggcccggcg gtgcgttttt
     9241 cggggttatc gcgggaaatg ttaatcctcg ctacgctgtt gctgttttgc gcagtgctac
     9301 tgatcgtgct ggtgggaacg ctttatccga tgatttacgg tctgctgggc tggggacgcc
     9361 tctccgttgg cgcgccgtat tttaaccgcg cgacgttacc gtttggtctg ttgatgctgg
     9421 tggtgattgt gctggcgacg tttgtctctg gcaaacgcgc gcagcttccg gcgctggtcg
     9481 ctcatgcggg cgtgctgtta tttgccgcag ggatcgtggt ttccagcgtc agccgtcagg
     9541 agatcagcct gaatttacag ccgggtcagc aggtgacgct ggcaggatac accttccgtt
     9601 ttgagcgcct cgatctgcaa gccaaaggca attacaccag cgaaaaagcg atagtggcgc
     9661 tgtttgacca tcagcaacgc attggtgaat taacgccgga gcggcgtttt tatgaagcac
     9721 gccgtcagca aatgatggaa ccgtcaattc gctggaacgg catccatgac tggtatgcgg
     9781 tcatgggtga aaaaaccgga gcggatcgtt acgcttttcg tttgtatgta caaagcggtg
     9841 tgcgctggat ctggggtgga ggattgttga tgattgcggg cgcattgtta agcggatggc
     9901 gggggaagaa gcgcgatgaa taaagggctt ctcacgctac tgctgttatt tacctgtttt
     9961 gcgcgcgctc aggtcgtaga cacctggcaa ttcgccaatc cgcaacaaca gcaacaggcg
    10021 ttaaatattg ccagccagtt acgttgtccg cagtgccaga atcaaaactt actcgaatcc
    10081 aatgcgccgg tggcggtcag tatgcgccat caggtttata gcatggtgtc ggaggggaag
    10141 aacgaagtcg aaatcattgg ctggatgacc gaacgctacg gagattttgt tcgctataac
    10201 ccaccgttaa cgggtcagac gctggtgttg tgggcgctgc cagtggtgtt gttattgctg
    10261 atggcactga tcctctggcg agtgagggct aagcgatgaa acagcctcaa ataccggtga
    10321 aaatgctgac aacactcacg atattgatgg tattcctctg tgtcggcagt tatctgttaa
    10381 gcccaaaatg gcaggcggta cgtgcggagt atcagcgtca gcgcgatccg ctacatcagt
    10441 ttgccagcca gcaaaccccg gaagcgcagc ttcaggcatt gcaggataaa atccgcgcta
    10501 atccacaaaa cagcgaacaa tgggcgttac tgggcgagta ctatctgtgg caaaacgatt
    10561 acagcaattc gctgctggcg tatcgtcagg cgttgcaact acgtggtgag aacgctgaac
    10621 tgtatgcggc gctggcgacg gtgctttatt accaggccag ccagcatatg accgcccaga
    10681 cttgcgcaat gatcgacaaa gccctcgcgc tggactctaa cgaaatcacc gctctgatgc
    10741 tgctggcttc cgatgcgttt atgcaggcga attacgcgca agccatcgaa ctatggcaaa
    10801 aagtgatgga tctcaactca ccccggatca accggacaca gctggtcgag tcgattaata
    10861 tggcaaaact gttgcagcgg agatcggatt aatcatctgg tgatgaagat tatgtcattg
    10921 agctgcataa aaaataatcg aatgaacata tgccaaaaat aatcactaat cagtattatt
    10981 gcagattaaa caaataaaaa tctttccata acaaatggtt attcattaat cctgccataa
    11041 actcgcgtta ttatgcatta atgcagcgaa aagctctgtt gttaaagggt tgcgcaacat
    11101 accgcgcaaa tgatactgat cataagcgtt aaaaaaatct acaaaccaac gcaacacaat
    11161 tcatgccctg gcagtatgtc acgttctcgc gtttctgaac ggggaacggc gctccattga
    11221 ggaagttatt ctgatgaaaa atataaaatt cagcctggcc tggcagattc tgtttgctat
    11281 ggtgctgggc attctcctgg gaagctacct gcactaccat agcgacagcc gcgactggct
    11341 ggtcgtcaat ttgctctctc cggcgggtga tatcttcatc catctgatca aaatgattgt
    11401 tgtgccgatt gtgatctcca cgctggtggt gggtatcgcg ggtgttggtg atgccaaaca
    11461 gctcgggcgt attggcgcga aaaccattat ttacttcgag gtgatcacca ccgtcgccat
    11521 cattttgggg atcactctgg cgaacgtctt ccagcccggt gccggggtgg atatgtcgca
    11581 gctggcgacc gtcgatatct cgaaatatca gagcactacg gaagcggtac aaagcagttc
    11641 ccacggcatt atgggcacga ttttgtcgct ggtgccgacg aacattgtgg cgtcgatggc
    11701 gaaaggcgaa atgctgccga tcatcttttt ctcggtgctg tttggtctgg ggctttcttc
    11761 cctgcccgcg acgcatcgtg aactgctggt gaccgtgttc cgctccatct ctgaaaccat
    11821 gtttaaagtg actcacatgg tgatgcgtta tgcaccggtg ggtgtgtttg cgctgattgc
    11881 ggtgacggtg gctaactttg gtttctcttc tctgtggcca ctggcgaaac tggtgctgct
    11941 ggtgcatttc gccattctgt tcttcgcgct ggtagtgctg ggaattgtgg cgcgcctgtg
    12001 cgggttaagc gtctggatcc tgattcgtat tctgaaagat gagctgattc tggcgtactc
    12061 cactgccagc tctgaaagcg tactgccgcg aattattgag aagatggaag cctacggagc
    12121 accggcgtcg atcaccagct tcgtggtgcc gaccggttac tcttttaacc ttgatggttc
    12181 gacgctgtat caaagtattg ccgctatttt catcgctcag ctgtatggca ttgacctgtc
    12241 catctggcag gaaatcattc tggtgctgac gctgatggtg acctcgaaag ggattgctgg
    12301 cgtgccgggc gtgtcgtttg tggtgctgct ggcaacgctg ggtagcgtag gtattccgct
    12361 ggaaggtctg gcgtttattg ctggtgttga ccgtatcctc gacatggcgc gtactgcgct
    12421 gaacgtggtg ggtaatgcgc tggcggtgct ggtgattgcc aagtgggaac acaaatttga
    12481 ccgcaaaaag gcgctggctt atgagcgtga agtgctgggc aaatttgata aaactgcgga
    12541 tcaataattg aagattgccg gggatatcca cccggcaatg tgtgaatgcc tgatgcgacg
    12601 cttgccgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc
    12661 cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt tgcttgttct
    12721 tctcgataaa accttcttca ccgtttaaga acatcattcc cgcccagtac tcggaataac
    12781 cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct
    12841 taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcagagt
    12901 cgttctccag atcttccgag gcgttttcta acagcgaaat agcctttgga taatccggtt
    12961 tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg
    13021 cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg accggattgg
    13081 tgattttgat ctgcgccagc agcgcgcagg catcagcatc accgctgtcg gcggctttct
    13141 gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat
    13201 attgtgcgcg tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt
    13261 cgttggcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga
    13321 taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct
    13381 ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt
    13441 tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg gcgatcggct
    13501 cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagttttcgg
    13561 taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt
    13621 tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcacccaaa
    13681 ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc
    13741 caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt
    13801 agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga
    13861 ttggcgctac ccagtcgcag gtgaagaact gcgccagacc gttcggggta tcaaacttct
    13921 ctttaaacag ataagaagtc ccctgatcgg catctgaagt atcgcggcaa ggccactgaa
    13981 tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac
    14041 gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg
    14101 cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga
    14161 agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg
    14221 ttgacggcaa aatgacatcc gccgccgacg cggttttggt cataaagatg tcctgaacga
    14281 taaccagttc cagatcttca aaggctttac gtactgccga cagctccgcg tcagtttgca
    14341 gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca
    14401 gctcgctgat gcgataaccg gtatgagcag gcaggctttc cacgccccag gctttggcga
    14461 atttctcgcg gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg
    14521 cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat
    14581 gcggcttacc gaggttacca gtcagcatcg cgaggctggt cagagaacgc acggtttcca
    14641 cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg
    14701 catacatccg tgccgcctga cgaatctcac tggcgctgac gccggtgata tcttcaaccg
    14761 actccggcgt gtagccttca acgattttac gatactcttc aaagccttct gtacgggaag
    14821 cgacgaacgc tttgtcgtac agattttctt caataatgac atggtccatc gcattcaaca
    14881 gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa
    14941 ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat
    15001 tcgccacgat tgggtgggaa tcagccgggt tgtacccgaa aacgaacact aaatcggtat
    15061 tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg
    15121 caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg
    15181 cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg
    15241 tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt
    15301 aattcagtgc ctcatcccag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca
    15361 tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat
    15421 aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga
    15481 ttttgccgtt atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg
    15541 tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac
    15601 tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac
    15661 tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa
    15721 ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc
    15781 ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc
    15841 cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa
    15901 gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc
    15961 ttccacgcgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt
    16021 gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt
    16081 gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa
    16141 tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg
    16201 gatggcatca aggccgaaaa acgctttgat atcaaagctc gggtagaaca gcgcccgcgc
    16261 ggaatccacc tgatctaatg acgcctgaac ataccagcgc atggcttgca gatccgggcg
    16321 tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc
    16381 caccggtttg atctccggca tatcgctggc ccccgcgcca atcaatgcac gcagagattc
    16441 tcgcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg
    16501 tgcccgcgcg ccgtggaaag gcacttgtgc ttccagaccg tgcgccactt tactctggtg
    16561 cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata
    16621 gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc
    16681 tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg
    16741 cacaccccac aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta
    16801 cggcccgtcc atacccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc
    16861 cgagacgcgt tggcggttga gcatccctaa cgctgcgacc tgtaattgtg aaccggcatc
    16921 taacaaatcg gcctgggact gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg
    16981 tgaaccactt agcgtccgtt ggatcagcgc atccagctac gggtcattga gttgtttcca
    17041 ccactgcgcc tgcggccagc cggagctggc aagatgaata tcatcggcca gtttgatttg
    17101 ttccggtttg agctgttgat gaggcgcaga atccttgcgt accaaggcac agccggaaat
    17161 cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat
    17221 tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc
    17281 aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc
    17341 tgcgaggcgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga
    17401 ccggcagcgt atttttccag ggcgtcggca aactgacctg ctctgtccgg ctgtagtgcg
    17461 ttatccagcg cctgcgccga attccaggtg gcatcccagg cgtgaaggat atcgcggccc
    17521 tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga
    17581 cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg
    17641 atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt
    17701 actttactta acatacccag cgcgccagcc agtttttgcg gcagcgtgcg cgcttcactt
    17761 tcaggccaga caaaggtgta aatcaccgcg gacaccacgg taccaatgat gatacccagg
    17821 gcgcgatcgc gaatttccac caggtcgtac actgggccaa aaacgttttc gagcgtggcg
    17881 agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca
    17941 atccatgcgc ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc
    18001 agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg
    18061 ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttggattaga gacgatcacg
    18121 caggtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtaaaa ggtgtaacag
    18181 atcaaacagg cgagcaatgt ttttaccgcg tagcgtatat agtctggatt ggtaaaagca
    18241 tcggcgacca ttgatggcgg tttggcggcg ggcgttggcg gcgtattcgg gttcatctgg
    18301 cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc cgccaccgct
    18361 tcactttcac tgatccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc
    18421 agcttgttga tttctgaggc taatttttgc cgaaattcaa taattgcctg agaatcagca
    18481 aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag
    18541 ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc
    18601 agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg
    18661 tctgtcaggt gactaatggc atcatcaagc cgatcattaa gcgcctgatg catttgcgaa
    18721 atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg
    18781 ccaacaacga tacaccacag cgttaagcgc acgaccactt ccggatagtc gagcatggcg
    18841 gggaaggttt gcccgtaaat ggcgacaatg gcgacagcga aaaagaccag tcccagccga
    18901 tgggtgcgca tcaaaaacat gcggcccatc aggatcggcc cggcgatgat caatcggatc
    18961 aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc cagcaccgtg
    19021 gcaaccacaa acaagatcgc cacaaatttg gtgtaaaacg cgttcgactg aataccgtaa
    19081 aacagcactg ccagcgataa cgccacaaaa gggatctcaa aagtcatcga gatcagaatc
    19141 accagcaggc agcctaccca gagttgcacg atctgcggca cgcggccggg tcgccgctcg
    19201 cttaactctt catgaaagaa cgccagcagc ctgaccatcg gtaatggcag ggagttgagc
    19261 gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg aacatttccg
    19321 ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg acccagttaa
    19381 tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcatcc ggtagcacgc
    19441 cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc atcaggcgaa
    19501 tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag ttggcgatca
    19561 cgtaccagtg acgagtgtcg attagggtaa aaatagggcg catggcagaa gcaaattgcc
    19621 cgacggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg gtcgccattt
    19681 ccagatgcag tttggtcagg gcaatatccg cttcgaccgc cgcacgttgc gccaccagcg
    19741 catcgacgcc gctgacggcg ctggcggctg actgcgcctg taacaatacg gcattaagat
    19801 cggcttctgc ggcgcgctgc gcagttcttg cacggtcaac gtcttccgct gagacaaaac
    19861 cctctttcag taatggctcg gtacggcgta atgtatctgt ggcctgtttc gcggcggcac
    19921 gggctttttc taccgtggca ttaatcgagt cggcaccaaa ctgttgcgca tcaacgctac
    19981 gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct ttcgccagat
    20041 tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg actgcctggt
    20101 tgtcggtgac cgccagttct acaatgcggc cgctgacttc cggcaccaca tcaatggtat
    20161 ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag ataacgaaaa
    20221 caagggcaac cagcgccaat gcaaccacta acagagcagg gaatttactg cgaggagctt
    20281 ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc agccagaaca
    20341 gcatggcgta gagagcaaaa agggcggtat aaataattcc gacaaatgcc agattgatat
    20401 ttgcccgctg aataactcgc ctcgtgatga gcatcaaaat aaggctggca atggcgcaga
    20461 aaaaccagct gggataataa gcaccgatca ccgggattgc cggagataat gagcatcccg
    20521 aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc atgcgagaga
    20581 gaactgttgg catattaaat acatcctgtc tgctgcgaac aatatctgta gtgaatatca
    20641 atatccattg aaggcgtcag t
//
LOCUS       DAZPLM010000063        20466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPLM010000063 DAZPLM010000000
VERSION     DAZPLM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.063"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            199..699
                     /gene="tssB"
                     /locus_tag="ACHVC7_001923"
     CDS             199..699
                     /gene="tssB"
                     /locus_tag="ACHVC7_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR2115986.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            734..958
                     /locus_tag="ACHVC7_001924"
     CDS             734..958
                     /locus_tag="ACHVC7_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2115987.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1009..2484
                     /gene="tssC"
                     /locus_tag="ACHVC7_001925"
                     /pseudo
     CDS             1009..2484
                     /gene="tssC"
                     /locus_tag="ACHVC7_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2491..2904
                     /gene="tssE"
                     /locus_tag="ACHVC7_001926"
     CDS             2491..2904
                     /gene="tssE"
                     /locus_tag="ACHVC7_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR2115988.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2908..4758
                     /gene="tssF"
                     /locus_tag="ACHVC7_001927"
     CDS             2908..4758
                     /gene="tssF"
                     /locus_tag="ACHVC7_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR2115989.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4722..5804
                     /gene="tssG"
                     /locus_tag="ACHVC7_001928"
     CDS             4722..5804
                     /gene="tssG"
                     /locus_tag="ACHVC7_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR2115990.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5829..7109
                     /gene="tagH"
                     /locus_tag="ACHVC7_001929"
     CDS             5829..7109
                     /gene="tagH"
                     /locus_tag="ACHVC7_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR2115991.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7106..7630
                     /gene="tssJ"
                     /locus_tag="ACHVC7_001930"
     CDS             7106..7630
                     /gene="tssJ"
                     /locus_tag="ACHVC7_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR2115992.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7633..8964
                     /gene="tssK"
                     /locus_tag="ACHVC7_001931"
     CDS             7633..8964
                     /gene="tssK"
                     /locus_tag="ACHVC7_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR2115993.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8969..9730
                     /gene="icmH"
                     /locus_tag="ACHVC7_001932"
     CDS             8969..9730
                     /gene="icmH"
                     /locus_tag="ACHVC7_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR2115994.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9739..12522
                     /gene="tssH"
                     /locus_tag="ACHVC7_001933"
                     /gene_synonym="clpV"
     CDS             9739..12522
                     /gene="tssH"
                     /locus_tag="ACHVC7_001933"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR2115995.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12519..13262
                     /gene="vasI"
                     /locus_tag="ACHVC7_001934"
     CDS             12519..13262
                     /gene="vasI"
                     /locus_tag="ACHVC7_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR2115996.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13267..14679
                     /gene="tssA"
                     /locus_tag="ACHVC7_001935"
     CDS             13267..14679
                     /gene="tssA"
                     /locus_tag="ACHVC7_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR2115997.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14788..18222
                     /gene="tssM"
                     /locus_tag="ACHVC7_001936"
                     /pseudo
     CDS             14788..18222
                     /gene="tssM"
                     /locus_tag="ACHVC7_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18233..19682
                     /locus_tag="ACHVC7_001937"
                     /pseudo
     CDS             18233..19682
                     /locus_tag="ACHVC7_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19609..20086
                     /locus_tag="ACHVC7_001938"
                     /pseudo
     CDS             19609..20086
                     /locus_tag="ACHVC7_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
     gene            20130..20466
                     /locus_tag="ACHVC7_001939"
                     /pseudo
     CDS             20130..20466
                     /locus_tag="ACHVC7_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4860 a         5580 c         5634 g         4392 t
ORIGIN      
        1 atttaacgct tgcagaatac ccctctttac gatcgataaa aagtcctttt cttatttatt
       61 ttgaagcgta aaatgcgggg tgatgaagga gtggtgattt tattttcacc gccattaggg
      121 cggcattgcc gatatttttt tgcggtacaa atatgccgtg ttcatggcct gtacgagata
      181 acatgacgga tcacttttat gagcaaaaaa tttgaaggca gcgtcgcgcc ccgcgagcgt
      241 attaatatat cgtatgtccc aaaaacagat ggccagacgg ctgaagtgga acttccgctt
      301 aacatgctcg ttgtcggcga taccggaaat acgcaggaaa catccccact ggatgaacgt
      361 caggcagttt ccgttaacaa gcacaatttc ggtgcagtga tggctgaagc agccattggc
      421 ctcaatttta ctgtccccgc cacgctgaag ggaagcacaa cagacgatga actgaatgtg
      481 gcgctgaata tcaaatcact ggatgatttt tcaccagata gcgtggcccg gcaggttccg
      541 gaagtgaata aattgctgga actgcgtgaa gcactgactg cactgaaggg gccgatgggt
      601 aaccttccgg cattccgtac ccagctccag gcattgctgg aaaatgaaga gtcccgcgag
      661 cagttgctca aagaaatcgg tctggtgagc aataaataac caccactatt tcattaaaca
      721 gggttatcca ggcatgacgg cacacaaaaa tatctccggg acataccacc tctctgttgc
      781 agacattctg caggtggttt accaggtatg tttcagcccg tccgttgaaa tcaaccagga
      841 tggtgtggct gcgctgatta caacactgga tcgccgtatc agtgatctat tggatgagat
      901 tatccatttt tgtgagtttc agcagagcgc gtcgcactgg cagcgcgtac ttcactgatg
      961 gcgaaaagcc ggtttcagaa acacagctaa aacagaagga ttttcagtat gtctttacag
     1021 gaagaggaac tcgtttccag tcatgccggg cagcctgagc aggcatcgtc gctgcttgat
     1081 caaatcatgg cacagacccg tattcagccc ggttcggaag gttatgacgt tgccctccag
     1141 ggtgtgacag cctttattgc cagcatcctg caatccactg catctgccga accggtcaat
     1201 aaactggccg tggacagcat gattgcagat attgatgaac gcatcagccg ccagatggat
     1261 gtcatcatcc atgcgcctgc ctttcagcag gtcgagtcgt tctggcgctc actgaaaacc
     1321 atggtggatc gcgttgattt ccgtgaaaac atcaaggtca acgtgcttca tgtcaccaag
     1381 caggaacttc tggaagactt tgagtttgcg ccagaaatta ttcagtccgg attttataag
     1441 cacgtttatt cgtccggttt cggacagttt ggcggtaaac ccatcgctgc cgtgctgggc
     1501 gcttatgagt tcaaaaatac cgcgcctgac atgaaactgc tgcaatacgt cagtgccgtg
     1561 ggcgcaatgg cgcacgcacc gttcctgtcc tccgtttccc cggagtttat ggggctgaac
     1621 tcgtggaccg aactgcctaa tatcaaagat ctttatgcca tctttgaagg tccggcctac
     1681 accaaatggc gcgccctgcg tgactcggaa gattcgcgct atttagggct tacggcaccc
     1741 cgcttcctgc ttcgccagcc ctattcacca acagataacc ctgttaagaa ctttaactat
     1801 tacgaagatg tcagccagaa ccacgaagat tatctgtggg gtaatacggc ctggatgctg
     1861 gcatgcaata tcgcagacag ttttgccaaa taccgctgat gtccaaacat tatcggtccg
     1921 caaagcggcg gcgcagtgaa agatctgccg atgcatctgt tcgaaacgat ggggcagatt
     1981 caggccaaaa tcccaaccga ggtgctggtc accgatcgcc gcgaatttga actggctgaa
     2041 gagggtttta tcacccttac catgcgtaaa gactctgata atgcagcctt tttctctgca
     2101 aactcggtac aaaaaccgaa gcactttccg ggaaaagatg cagaaaccaa ctataagctg
     2161 ggtacgcagc ttccttatct cttcatcatc aatcggttag cgcactacat caaggtgttg
     2221 cagcgtgaac aactggggtc atggaaagaa aggagcgact tagaaagaga gcttaatacc
     2281 tggatccgtc agtatgttgc cgaccaggaa aacccgcctg cagacgtgcg cagccgcaaa
     2341 cctctgcgcg ctgccaaagt tgaggttatg gatgtagaag gcgaacccgg ctggtaccag
     2401 gttgcgctaa gcgtgagacc tcattttaaa tttatggggg caaattttga gctttccctg
     2461 gttggccggt tagacaggga gtaacacacc atgataccgg atcgggaaag aaccacaggt
     2521 tctctttttg agcgtatgga agcctcttcg gccagaaacc ggcagggagg cagtattcat
     2581 tcgctcaggc aatccattcg ccagaaccta cgaaacattc ttaatacccg gtccggtagt
     2641 tgccggggtg cgcctgagct gggcattgat gaaccggaag gggctgaaaa cttccgcgaa
     2701 tccatgagtc gggccatcga gcaatgtata gaacgctatg aaccccggat ctcacacgct
     2761 gaagtgcagg cagtggtttc atccgcctcc agcccgctgg atatgacgtt tcatattaca
     2821 gcctgggtga catttaacga aacgcatgag gtgcttgaat ttgatatggc accgaatggc
     2881 agccagcatt accgggtgga ctgacaaatg gaatttgaag aacgctattt ccgggaagaa
     2941 ctcgattacc tgcgccagct tagcaagctg ctggcaacgg aaaaacccca tctggcccgc
     3001 ttcctggccg aaaaagatgc agatccggat attgaacgcc tgctggaagg ggtggctttt
     3061 cttaccggca atctccgcca gaaaattgag gatgaattcc cagaactgac gcacgggctt
     3121 attaagatgc tatggcctaa ttacctgcgt ccggttccgg caatgaccct tattgaatat
     3181 acgccggata tggataagtc ttctgtaccg gtgttaatcc cccgtaatga gcagtttaca
     3241 accaacgccg gggaaatcag agttgatgaa gtgctgccct ctgatgctaa aaaggaggag
     3301 ccgcctccct gtaccttcac cctctgccgg gatatctggc tgctgcccgt tcgcctggag
     3361 cagattgaaa accgcagtac gacccgtaat ggtgttatca acatcacctt ttcggtcgca
     3421 ccgggaacgg acttccgcac gctggatctg aacaaacttc gcttctggct cggcaatgac
     3481 gacaactata cccgtgacca gctttattta tggttctgcg aatacttgca gggtgccgac
     3541 ctgactgtgg gtgaacagca tattcgcctg cctgagttta tgctaaaagc tgtcggtttt
     3601 gagccgcagg atgccatgct gccctggccg aaaaacgtcc acagcggcta ccggatcctt
     3661 caggagtatt tctgttaccc cgatgcgttt ctcttttttg atctttgtgg ttgtccggct
     3721 ttgcctgacg gattgcaggc ggaattcttt accctgcaac tgcgtttttc gcgccctttg
     3781 cccgtggaca tccggctgcg ccgcgattcc ctgcgcctgt attgcgcacc tgccattaat
     3841 ttatttatcc accatgcaga agccatcacg ctggacaacc ggcgggcaga ctatccgctg
     3901 gttcccagcc gccattaccc acaacattac gatgtatttt ccgttaacag tgtggtgagc
     3961 caggtccagg atatgttcag gaaaaaagat ctggggcgtc ctgtttcgac gcaggccgcg
     4021 cgccagtggc cagcctttga aagtttcagc catcagatag aatacagccg gaagcgggaa
     4081 gtggtgtact ggcatcaccg gaccaaaaca tccctgttcc atcgcggctt tgatcatacc
     4141 cttgccttta tacatgctga tggcagttat ccgtcagacg aatctctgct cagtaatgaa
     4201 gtggtttcgg tatcgctgac ctgtaccaac cgtgagcttc cgtcacaaat tcgttccggc
     4261 gatatcaccg gcacaaccgg aaaaaatgcg gctgttgctt catttcgcaa cattacccgc
     4321 ccgacgcaac cactctggcc ggtcattgat ggcagcctgc actggcccct actctccgcc
     4381 atgaacctga attatctgtc attactggat acggacgcgc tgaagcaggt catcgccaac
     4441 tttgatcgcc acgcaatcca tcatccgcag acggcacggc tgtcacaaca aaagctggat
     4501 gccattgagc gtctggagac ccgccccgtt gatcgcctgt ttacgggtat tcccgtccgg
     4561 gggctggctt ccacgctgta tctgcacccg gagccgtttg tctgtgaagg ggaaatgtat
     4621 ctgctcggta cggtgctttc gcattttctg tcgctgtacg ccagcgttaa ctcattccac
     4681 atgctgaccg ttgtgaacac agaaagccag gagacatgga aatggacgga aagaatcggg
     4741 cagcatcctc ttatctgagt ccgggaaatc ccccggcaga taaagagcag aatgatccgc
     4801 tggcacaggt tttccataat gcctgctctt acaatttttt tgcgatggcg gagctgttgc
     4861 accgcctggc aaagggtgaa aaggggacgc cagaattatc cctgcgcgac gatccggcac
     4921 aggaaaccct gcgtttcagc gccgatgcca gccttgcctt cccgtgcagt gatatcagtg
     4981 cgctgaaacg ggatacgtca ggggcattca ggatgaccac cacctttatg gggttacagg
     5041 ggagccagtc gcctctcccc ggttattacc tcgctcacct ggcctggaaa gcggtacatg
     5101 aacaaagtcc ggtaggtgat tttctggata tgttcagcca ccgcctgacc cagtttgtct
     5161 ggcatatctg gcgtaagtac cgctatcaca tcagttttcg taacggcggt gtggatgcgt
     5221 tctcgcagcg catgtactcc cttgtcgggc tgggccaccg ccagcttcgc gataagctgg
     5281 cgattaatca cagcaaaatg ctggcgtatt ccgggatcct ggcaaatccg ggacgatccc
     5341 cggaaattat ctgtggcctg gtgtcccact gctttgattt gtcagaagtg acactgcaaa
     5401 actggcaacg tcgcaaggtg gatattgaac cggatcagca aaatagtctg gggagctatt
     5461 ctcttaaaaa tggcgaaaaa ctggcgggaa gatcggtact gggcaacttt gttctgggta
     5521 cacgagtgcc cgatctcagc ggaaaattcc agctcagtat taccagcctg acccgaaaac
     5581 agttcctctc ttttctgccg tccggcgaaa acttcctgcc actgacgatg ttcgtgtcct
     5641 tcatcctgcg cgatcagctt gcctgggact tacatctggg gctggcgccg gagcaggtgg
     5701 gcgcaatgcg cctgggcgat aacaaaagtg cgctgctggg ctggaccagc ttcctcggca
     5761 caccggaaga acgaccctca gtcaccatca gggtacggtc gtaaattatg ataatcacgg
     5821 agcagaatat gccggaagag aaactacaga cgctttcatt gcaggtcatt aacggcagtg
     5881 agctggaaag cgggcgggcg gcgcgctgtc tgttcacaca gcagggaaat gtcggccatg
     5941 gccccgaatg ccactggtcg gtacaggatc gtcagcagag cattccggcc caggctttta
     6001 ccgttatcct gcacgatggc acattttgtc tgcgcccgca gacggcacaa ctgtggctga
     6061 atcaggcaaa agtcacagca acatcagacc taatacagtt gcgccagggc gatgagatcc
     6121 agatcggacg gctgatggtg agggttcatc tgaaccgggg agatattccc cattacgatg
     6181 aggaaatggc cactcccgaa accatcgtta ccaatcgcga tatgctcacg gataccctgc
     6241 tatcaacgga gggtgcgcca cactatccgg gaatgactca ccggcaccag cttgcagaca
     6301 ccgtggtaaa cggtttttct gccgatccac tccaggctct tcagtccgaa agcctgatta
     6361 ccacgggcga tccgctttca ggcattgcgg ctgtccggcc atcggcaccg ctgtccgatc
     6421 cggcaagtaa tggggggatc aatactccgt ttatggatct gccgcccatt tatgccagcc
     6481 ccggcgatcg taatgacgac gtctctgcgg cagaaatggc gcaacgccac gttgcggtca
     6541 cccccttact gcgcggtctt ggcggctcgc ttaccatgag caattccgac gatgcggatg
     6601 attttctgga ggaggccgga cgaacgttac aggccgcaat aaaaggtctg ctcgatttgc
     6661 agcagcagcg taacagcctc tcagacaaac atttgcgccc gctggaagat aacccgctgc
     6721 gcctgaacat ggattacgcc accgcgctcg acgtaatgtt tgccgaaggt aaaagcccgg
     6781 tacatctggc ggctcccgcc gccgtcagtg aaagcctgcg caatgtccgc caccacgaag
     6841 aagctaaccg ggcagcgatt gtggagtcgc ttcgtgtcct gctggatgct ttctcaccac
     6901 aaaatctgct gcgccgcttt gtgcagtacc gccgcagcca tgaactgcgc cagccgctgg
     6961 atgatgccgg agcatggcaa atgtacagcc attattacga agaactggcc tccgatcgcc
     7021 agcaggggtt tgagatgctg tttaacgagg tctacgccca ggtctatgac cgggtgcttc
     7081 gtgaaaaaca gcgggagccg gaagcatgaa caggaaggcg tttctggcct gcgtgctgat
     7141 gtgcgtcata ttaaccggct gcgaaacggc gaaaaaaatc agtgaggtga tcaaaaaccc
     7201 ggatatccag gtcgggagcc tgaaggagca accttcggaa ataaccgtca ccctgctgac
     7261 cgaaccggac accaatacca atgcggaagg tgaatcagcc gccgtggatg tgcaactggt
     7321 gtacctgact gacgactcaa aattgctggc agccgactat gaccagatag ccagcacgcc
     7381 gctaccggat gtgctgggta aaaactatat cgaccaccag gatttcaacc tgttgccgga
     7441 caccatcaaa accctgccgc cggtaaagct ggatgagaaa acgcagttta ttggtgtcgt
     7501 ggcctatttc tccgatgacc aggccactga atggaaacaa atcgagacgg tggaaggaat
     7561 cggccaccat taccgcctgc tggtgcatgt acgtcaaagc agcatcgaaa tgaaaaaaga
     7621 ggatgaataa ttatggcaac cacccgcaac aaggtgatgt ggcaggaagg gatgctgatg
     7681 cgtccacacc atttccagca gcagcagcgt tacaacgact acctggataa ccagcgtttc
     7741 cgggccatga atgatttatc ctggggattt accgaactca ccctcaacaa tgaactgctg
     7801 gcgcagggta agatcatgat tgacagcgcg tcaggcacac tgcccgacgg caccgtcttt
     7861 tctatccccg accaggacgc actgcccgat ccgctgcacc cgcaaaattt tccggacgaa
     7921 agaagccgca atatctacct cgctctgccg gtcgccagtg atgtgagaaa tgaaatcagc
     7981 gacggacggc gaatcgggcg ttaccggctg aattatgccg atgtccggga tttgcattca
     8041 gaagaaggtg acgcgcgaac attgacgctg ggtcaactga cgccgcgcat tatgagcggt
     8101 gcagaagata tgagcgccta tattacgctc ccgctttgcc gtatcagcga tcgccatgcc
     8161 gatggctccc tgacgttgga tgacgatttt atcccctcct gccagaatat tcaggtcagt
     8221 aagaaactgc gtgtttatct caaagaggta cagggggcca ttggcggacg ggcaagcgat
     8281 ctggcaaacc gtattggctc tccggcgcag agcggcatcg cggatgtggc ggaatttatg
     8341 atgttgcagt tacttaaccg taaccagacc cggtttaccc atcacgcccg tcgatcccag
     8401 ctccacccgg aagatttcta ccttgatctt gccgggttgc tgggtgaact gatgaccttt
     8461 accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg atcatcatga cctgaccaaa
     8521 atatttaaaa cgctgttacc ggaagtcaaa cgggcgctgc ataccgtact gtcgccaaga
     8581 gccgtcaatc tgccgctgca tctgcgtgac ggtatctggc aggccgatat ccatgacaca
     8641 gaactgctgc aatctgccac ctttgtgctg gccgtggcgg caaatatgcc ggtcgatcag
     8701 atccagcgcc agtttatcca gcagtcgaaa atttcctcgc cggaaaaaat ccgcaatatg
     8761 gtcagtgtgc agatcccctg tattccattg cgtgccctta tggtggctcc ccgccagctt
     8821 ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga gtggccaggc ctggacagaa
     8881 atggctgccg ccggggccgt tgcactgcat gtttccggca gtttcccgga tctgaacatg
     8941 caactgtggg cgataagagg gtaagacgat ggatgagggg tcactgtctc tgccaccatt
     9001 taccggacac gacgaaaaaa gccagcgcaa ctaccatctc gcgcttcgtg gcaacagcct
     9061 gaatccgatg atcgacgcgg ccacgccgct actcggtatg gtaatgcgcc tttcaaccat
     9121 gaacagccag acgatgccgg agcatctttt tgctcaggtg gtgacagatg ttcaggccgt
     9181 ggagcaactg cttcaggagc agggctacga accgggcgtg attatctcgt ttcgctacat
     9241 cctctgcacg tttattgatg aagcggcgct cggtaacggc tggtcaaaca agaacgagtg
     9301 gatcaagcag tcattgctgg tccatttcca caacgaagcc tggggtggcg agaaagtttt
     9361 catcctgctg gagcgcctga tccgtgaacc caaacgttac caggatctgc tggaatttct
     9421 ctggatctgt ttttctctgg gatttcgcgg gcgctacaaa gtggcagcac aggatcaggg
     9481 ggaatttgag cagatctacc gccgtctgta ccacgttctg cataaactgc gcggcgatgc
     9541 ccctttcccg ctgttacacc aggacaaaaa aacccagggc gggcgttatc agctcatcag
     9601 tcgcctgact gttaaacata ttttttgtgg tggcgttgtc gtgctggcgt tgttttacct
     9661 gttctatctg ttgcggctgg acagccagac gcaggacatc ctgcaccagc taaacaaatt
     9721 acttcgataa ggacgtttat gatccagatt gatcttccca cgctggtaaa acggctgaac
     9781 ctgttctccc gccaggcgct ggagatggcc gcctctgaat gtatgagtca gcaggcagcg
     9841 gaaattaccg tcagccatgt gctcattcag atgctcgcca tgccacgcag tgacctgcgg
     9901 gttattaccc gccagggcga tattggcatg gaagagttgc gccaggcgct gacggtagag
     9961 aactacacaa ccgcccgttc tgcggacagc tacccggcgt tttccccgat gctggttgag
    10021 tggcttaaag agggctggct gctggcgtcg gctgagatgc agcacagcga actgcgcggc
    10081 ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt atataccgcc tgctgccgcc
    10141 cggctgttga ccggcattaa ccgtgaccgt ctgcaacagg actttgtgca gtggacacag
    10201 gagtcggcgg aatcagtcgt gccggatgca gacggtaaag gcgcaggcac aatgacggac
    10261 gcctctgaca ccctgcttgc ccgctatgcc aaaaacatga ccgcagacgc ccgtaacggc
    10321 aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc tgatgatcga cattctctgc
    10381 cgccgccgta aaaacaaccc ggtggtggtg ggcgaagcgg gcgtgggcaa aagcgcactg
    10441 attgaagggc tggcgctgcg catcgtggca ggccaggtgc cggacaagct gaaaaacacc
    10501 gatatcatga cccttgactt gggcgcattg caggccgggg cgtcggtgaa gggtgaattc
    10561 gaaaaacgtt tcaaagggct gatggcggag gtcatttcct ccccggtgcc ggtcattctg
    10621 tttatcgacg aagcacatac cctgattggc gcgggcaacc agcagggcgg gctggatatc
    10681 tccaacctgc tcaaaccggc gctggcgcgc ggcgagctga aaaccatcgc cgccaccacc
    10741 tggagcgagt acaaaaaata cttcgaaaaa gatgccgccc tgtcgcgccg cttccagttg
    10801 gtgaaggtca gcgaacccaa cgctgccgaa gccaccatta ttctgcgcgg tctgtcggcg
    10861 gtctatgaac agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc cgctgcgaca
    10921 ttaagcgagc gttatctctc cgggcgtcag ttaccggaca aagcgattga tgtgctggat
    10981 accgcctgcg cccgtgtggc catcaacctg tcgtcgccgc cgaagcaaat ctcggcgctg
    11041 accactctga gccaccagca ggaggcggaa attcgccagc ttgagcgcga gcttcgcatc
    11101 ggactgcgta ccgacacatc acggatgacc gaggtgctgg tgcagtatga tgaaacgctg
    11161 acggcgctgg atgaactgga agcggcctgg caccagcagc agacgctggt ccgggagatt
    11221 atcgcgctgc gccagcagtt actgggcgtg gcagaggacg atgcggcgcc gttgccggac
    11281 gcagataccg tggaggatac gcagccagag tcagagtcag agtcagagtc agagtcagag
    11341 caggataata ccggtgccga accggctgat gaggccgaca gagaacaacc ggaagagacc
    11401 gctgaaacag tttccccggt acagcggctg gcccagctca ctgccgaact ggacgccctg
    11461 cataacgacc ggttgctggt ctccccgcac gtcgataaaa aacagattgc ggcggtgatt
    11521 gccgaatgga ccggcgtgcc gctcaaccgc ctgtcgcaga atgaaatgtc ggtcatcacc
    11581 gacctgacga aatggctggg tgacaccatc aaaggccagg acctggcgat tgccagcctg
    11641 cataagcatc tactgaccgc acgcgccgac ctgcgtcgtc cgggacgccc gctcggtgcg
    11701 ttcctgctgg ctggccccag cggtgtgggt aaaaccgaaa ccgtcctgca actggcagag
    11761 ctgctctacg gcggtcgcca gtacctgacc accatcaata tgtccgaatt ccaggagaaa
    11821 cacaccgtct cgcggctgat tggttcgcct ccgggctacg ttggctacgg tgaaggcggc
    11881 gtactgaccg aagcgattcg ccagaaaccc tactcggtag tactgctcga tgaagtggaa
    11941 aaagcgcacc cggatgtgct caacctgttc taccaggcgt tcgataaggg cgaaatggca
    12001 gacggtgaag gccgcctgat tgactgcaaa aatatcgtct tcttcctgac gtccaacctc
    12061 ggctaccagg taatagtcga gcatgccgat gacccggaaa ccatgcagga agtactgtat
    12121 ccggtgctgg ccgacttctt caaacctgcc ctgctggcgc gtatggaagt ggtgccgtat
    12181 ctgccgctgt cgaaagagac gctcgccacc attatcgccg gaaaactggc ccgtctggat
    12241 aacgtgctgc gcagtcgctt tggtgtagaa gtggtcattg aaccggaagt gacggacgaa
    12301 atcatgagcc gcgtcacccg cgcggaaaac ggcgcgagga tgctggaatc ggtcatcgat
    12361 ggcgacatgc taccgccgct ctcgctgctg ctgttgcaga aaatggcggc taacacggcg
    12421 attgcccgga ttcggttgtc ggcagtggac ggcgcattta cggcagacgt ggaagatgct
    12481 cagaacgacg agtccgtcac aaaggatgaa acggttttat gagcaggttg cgcgttttcc
    12541 ggcagacagg atggtttatc gcgggcctga tgacgggctt acccgccact gccgcgcccg
    12601 cagaagcatc gtcaatggcc ggtgtggcgg tcgccgtcgc caccacgacc ccgccggatg
    12661 cgacagccac gttacaggcc atgcagtcct gtcggcggga gtccgcggcg ctggagcggc
    12721 ttgactgcta tgaccgcctg ctggcaccgc tgtccccgtc agggtttgac ggggcgctgg
    12781 tcaaagccgg ttttgtgggc gaggcctgga cccgcgccac agagcaggaa aaacgccgcg
    12841 agggcaacac cacagaactg ctggtgacgc aggtgccggg ggagcgcccg acggtggtga
    12901 taaccacccc ggctatcggt catgttccgc cacgcccggt gctgatgttc agttgcgtgg
    12961 acaatatcac ccggatgcag gtggcgctga tgcaccctct ggatgtccat gatatcgccg
    13021 tcactctgaa tgccgacaac cgggcattgc gcagccactg gtttgtgcgc gagaacggca
    13081 ccctgctgga gtccagccgg gggttatccg ggattgatga aattaagcaa ctgttcggcg
    13141 caaaaacgct gacggtggat accggcgcag acaatgccgc cggaaagctg acctttaaca
    13201 ttgacgggct ggcaagggcg attgctcccc tgcgcgatgc ctgccactgg gcgggagaat
    13261 aaaaatatgg caatggactt acgcgatccg aatgtctgga tatcgcacct gctggaaaac
    13321 ctgccggaag agaaactggc atcggcgctg aaagatgaca acccgaactg ggagtatatc
    13381 gacggcgaaa tcgtcaagct gggatcgctg gcccacagtc aactggatat cccggaactc
    13441 cagcgccggg ggctggtcat tctggcttcg gagagtaagg attttcgtct gctggcgcat
    13501 ctgctaagaa ccctgcaaca cgccggtgat cctcatctcg cattgcgcct gctggcgctg
    13561 tatgtggagc attactgggc ggtggctgcg ccgcagaata tggcgcataa aaaacgcttt
    13621 gccagccagg tcattaaacg ctttgaaaca ggtattgaag ttttttcaca gaacgccgcc
    13681 acagcgcagc gcgatgcgct gtcagggcaa ctggcaaaac tggcgcaatg ctggcagtca
    13741 cataacgccc cggaactggc acaggctact gatgatcttt ttgccctgta ccagcgtgcg
    13801 tttaatcgtg cggcaccggc tcctgtcccc actccggcgg cctccggtag ttcaccacag
    13861 accaccgcca cgtctgaaag cggcgtgacg caacccagtg ctccggctcc ccaaatcgtc
    13921 atcgacagtc acgacgacaa agcctggcgc gacacgctgt taaaagtggc ggccatttta
    13981 tgtgaacgcc agccggattc accgcagggc taccgtctgc gccgccatgc gctttggcag
    14041 aacatcacca gtacgcccca ggcggaaagc gatggccgta ctccactggc tgcggtctcc
    14101 gccgatatgg tggcagatta ccatgctcag ctcggtagtg ccgatatggc gctgtggcag
    14161 caggtggaga aaagcgtact gctggctcct tactggctgg atggtcactg cctttctgca
    14221 cagacggcgc tgcgtctggg ttacaaacag gtggcagacg ccatccgcga tgaggtcatc
    14281 cgcttccttg agcgtctgcc ccagcttacc gggctgctgt ttaatgacca cacaccgttt
    14341 atcagtgagc agacgaaaca atggctggca gcttcgcccg acgccaaagt tgcaccggtt
    14401 gcgcaaatcg gtgaggaatc gaaggcagcc agagcctgtt ttgctgagca gggtctggag
    14461 gcggcgctgc gatatctgga catgctaccc gaaggcgatc cccgcgatca gtttcaccgc
    14521 cagtaccttg ccgcacagtt gacggaagag gcggggctgg tacagcttgc gcagcagcag
    14581 taccggatgt tgttcaggat gggattgcag atgatggtgg cagactggga gccgtccctg
    14641 cttgagcagc tagaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg
    14701 ttcagattac caacaccccg actactcagc ggactcaaat cagccctgcg accggcgatg
    14761 ccccggttta aagtctctgc tttctggctg ctgatactgg cgtggatttt tctgctggtg
    14821 tggatctggt ggaaaggccc gatgtggacg ctgtatgaag aacagtggct caaaccactg
    14881 gcgaaccgct ggctggcaac ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc
    14941 cgggtgatga agcgcctgca acagcttgaa aaaatgcaaa agcaacagcg cgaggaagcc
    15001 gttgatccgc tcagcgtgga actgaacgcc cagcagcgtt atcttgaccg ctggctgctg
    15061 cgcctgcaac gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc
    15121 atcggcccgg cgggcagtgg caaaacgaca ctgctgcgca aggggtttcc gtccgacatt
    15181 atttatgccc cggagggcgc acgcggcgca gaacaacgcc tgtacctcac gccccatgtc
    15241 ggtaaacagg cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat
    15301 atcctgcatc gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc
    15361 cagccgctga acgggattat tctgacactc gatttacccg atctgctgac cgcagacaaa
    15421 cgccgccgcg agcatctgtt acagacgctg cgcagccgct tacaggatat acgccagcat
    15481 cttcactgcc agttaccggt ttacgtggta ctgacccggc ttgatttatt acagggtttt
    15541 gccgccctgt tccagtcact caacagacag gatcgcgatg cgattctggg cgtcacgttc
    15601 acccgccgtg cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca
    15661 tgggtggatc gaatgaatct ggcgttgccg gatctgatgg tcgctcagac tcacacccgc
    15721 gcgtctttat tcagtttttc ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg
    15781 cttgagggtc tgcttgatgg cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc
    15841 tcttcgcttc agcgtggaca gatggatgat atattcaccc agtctgccgc ccgccagtac
    15901 cggctgggca ataacccact ggcgtcctgg cccctggtgg acaccgcgcc ttatttcacc
    15961 cgcagcctgt tcccgcaggc attactcgca gagcctaatc tggcaacaga gagccgcgcc
    16021 tggctgatac gttcccgtcg ccgcctgacg gttttctctg ccacaggcgg cgtggcagca
    16081 ctgctgctca tcaccggctg gcatcactat tacaacggta actatcagtc cggcatcacc
    16141 gtgcttaagc aggccaaagc ctttatggac gtgccgcctc cgcaggggga agatgacttt
    16201 ggcaacctgc aactgccgct cctgaacccg gtacgcgatg ccacactggc ctatggcgac
    16261 tggggcgacc gcagccgtct ggccgatatg ggactgtacc agggacgacg tatcgggcct
    16321 tatgtggaac agacctatct gcaactgctg gagcaacgtt acctgccctc gctgtttaac
    16381 gggctggtca aagcgatgaa cgccgcgccg ccggagagtg aagaaaaact cgcggtgctg
    16441 cgcgtgatgc gaatgctgga ggacaaaagc ggacgtaaca atcaggtggt gaagcagtat
    16501 atggcaaaac gctggagcga aaagttccac ggtcagcgcg atatccaggc acaactgatg
    16561 tcccatcttg actacgcgct ggctcatact gactggcacg cagagcgtca ggcgggcgac
    16621 ggtgacgcca tcagccgctg gacgccatat gacaagcccg tggtatcagc acagaaagaa
    16681 ctgagcaaac tgcctgtcta ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc
    16741 gttcttcctg ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt
    16801 acatctgccg atgacaacaa actggttgtt ccacagttta tcacccgcta cggcctgcaa
    16861 agctattttg taaaacagcg cgatgaactg gttgaactga cagcgatgga ttcctgggta
    16921 cttaacctca cccgcaatgt gaaatacagt gacgccgacc gtgcggaaat ccagcaccag
    16981 ttgaccgagc agtatatcag cgactacacc gccacctggc gggctgggat ggacaatctg
    17041 aatatccgca attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc
    17101 ggcgaccagc ctttgcagcg ggcgctgacc gtgctgcgtg acagcacaca gccaggcgtc
    17161 ttttctgaaa aactctctgc caaagaacgg gaggaagccc tggcagagcc ggattaccag
    17221 ttactcaccc gcctcgggca tgaattcgcc ccggaaaaca gtaccctggc agtacagaaa
    17281 gacaaagaaa gcacgatgca ggccgtgtat cagcaactca ccgagttgca ccgctacctg
    17341 ctggcaatcc agaacgcgcc tgtaccaggg aaatcggcgc tgaaagccgt gcagttacgg
    17401 cttgatcaga acagcagcga tccgatattc gccacccgcc agatggcaaa aacgctgcct
    17461 gctccgctca accgctgggt tggcagactg actgactagg cctggcatgt ggtgatggtg
    17521 gaggctgttc attatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag
    17581 caactggcaa ataactatcc gtttaatccg cgttctgcac aggatgcctc actggatgcc
    17641 ttcgaacgct tctttaaacc ggatggcata ctggatacct tctaccagca gaacctgaag
    17701 ctgtttatcg ataatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa
    17761 gatattattg cgcaactgga aactgcgcag aaaatccgtg acatcttctt cagcaaacag
    17821 aacggtctgg gaacatcctt tgccgtggaa acggtatcgc tttcaggcaa taaacgccgc
    17881 agtgtactga accttgacag tcagttagtc gattacagcc agggccgtaa ctataccgcc
    17941 catctggtct ggcctaacaa catgcgcgaa ggcaacgaaa gtaagctgac gctcatcggc
    18001 accagcggca acgcgccgcg cagtatcagc ttcagcgggc cgtgggcgca gttccgcctg
    18061 ttctgggccg gacaactgac cggagtacag gatggcaact ttaccgtgcg ctttagcgtg
    18121 gacggtggcg cgatgaccta ccgtgtgcat accgacacgg aagataaccc gttcagcggt
    18181 gggttgttca gccagtttgg tctgtcagac acactgtact gaggaggcag ggatgaatag
    18241 taacgtactg acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag
    18301 cgccatccgc gaggaaataa acaaagccag ccacccgtca cagcctgagc tgaactggaa
    18361 actggtggag tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc
    18421 cacctactat acgcttgccc ggacacggac acagggactg gcgggattct gcgaaggtgc
    18481 ggaactgctg gcggcaatgg taagccacga ctgggataag ttctggccgc agggcggccc
    18541 ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca
    18601 aatctccttt gcggaatccg acctaccact gatctaccgc acagagcggg cattgcagct
    18661 tatctgcgat aagctccagc aggtggaact gaagcgcgtt ccgcgcgtgg agaatctgct
    18721 ctattttatg cagcacacgc gtaaacggct tgaaccccag ccgaagagta acgctgagaa
    18781 cgccgcacag accacggtca gaacgctgat ttatgccccg gaaacactgg catcttccac
    18841 accagaagcg gtagtgcctc ccctgcccgg cctgcctgag atgaaagtgg aagtgcgcag
    18901 tctgacagag aatcccccac aggccagtgt gataaagcaa ggcagtacgg taagagggtt
    18961 tatcgcaggg atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta
    19021 tccggtgcag cagcaactgt tacaggttaa cgacaccgct cagggcgcag caacggtgtg
    19081 gatggcctca cctgaactcg aaaactatga gcgcaggctg caacaacttc ttgatacctc
    19141 cccggtacag ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc
    19201 ggaaagcctg caacagcaac aggcctcgac acaatggaat gaggcactca aaacccgcgc
    19261 acagagtagc ccgcagttgc gtggctggtt gcagacccgc caggacttac atgcttttgc
    19321 agatctagtg atgcagcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat
    19381 ctggcaggcg gagcggggac tggggcagga aacccccgtt gagtctctgt tgacgcagta
    19441 ccatgatgcc cgtgcgcaga agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg
    19501 actcgaaggc gtgttaagcc gctggctgct gctgaagaat aacgtcatgc cagaggcggc
    19561 aaccggtacc acggccgaaa aataattatt taattaaagg agattatcat ggcaaatata
    19621 agttatttat ctttgagtgg agagacacaa gggctaattt cagcaggttg ctcaacgctt
    19681 gattctgtcg gaaacaaagc gcagccagaa cataaagatc atggtttatg cgttaatgca
    19741 ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga
    19801 taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga
    19861 atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct
    19921 gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga
    19981 actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt
    20041 agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac
    20101 ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac
    20161 agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc
    20221 agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc
    20281 agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaaaa aaaaaaaaaa
    20341 atactcagcc caataacatc atttgattac attcatttta tcactacaca tccttcaggt
    20401 ataaaagata cactcgcctg gttagttaac gcgagtaaat taatgactga gtttgatgac
    20461 aatggg
//
LOCUS       DAZPLM010000064        20095 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPLM010000064 DAZPLM010000000
VERSION     DAZPLM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.064"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            168..839
                     /locus_tag="ACHVC7_001940"
     CDS             168..839
                     /locus_tag="ACHVC7_001940"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR2115998.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            849..2045
                     /locus_tag="ACHVC7_001941"
     CDS             849..2045
                     /locus_tag="ACHVC7_001941"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR2115999.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2045..2605
                     /locus_tag="ACHVC7_001942"
     CDS             2045..2605
                     /locus_tag="ACHVC7_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR2116000.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2607..3380
                     /locus_tag="ACHVC7_001943"
     CDS             2607..3380
                     /locus_tag="ACHVC7_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR2116001.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3568..3843
                     /gene="frmR"
                     /locus_tag="ACHVC7_001944"
     CDS             3568..3843
                     /gene="frmR"
                     /locus_tag="ACHVC7_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR2116002.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3878..4987
                     /gene="frmA"
                     /locus_tag="ACHVC7_001945"
     CDS             3878..4987
                     /gene="frmA"
                     /locus_tag="ACHVC7_001945"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR2116003.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5080..5913
                     /gene="fghA"
                     /locus_tag="ACHVC7_001946"
     CDS             5080..5913
                     /gene="fghA"
                     /locus_tag="ACHVC7_001946"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR2116004.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6139..6678)
                     /locus_tag="ACHVC7_001947"
     CDS             complement(6139..6678)
                     /locus_tag="ACHVC7_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR2116005.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6780..7991)
                     /gene="mhpT"
                     /locus_tag="ACHVC7_001948"
     CDS             complement(6780..7991)
                     /gene="mhpT"
                     /locus_tag="ACHVC7_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR2116006.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8278..9291)
                     /gene="mhpE"
                     /locus_tag="ACHVC7_001949"
     CDS             complement(8278..9291)
                     /gene="mhpE"
                     /locus_tag="ACHVC7_001949"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR2116007.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9288..10238)
                     /gene="mhpF"
                     /locus_tag="ACHVC7_001950"
     CDS             complement(9288..10238)
                     /gene="mhpF"
                     /locus_tag="ACHVC7_001950"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR2116008.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10235..11044)
                     /gene="mhpD"
                     /locus_tag="ACHVC7_001951"
     CDS             complement(10235..11044)
                     /gene="mhpD"
                     /locus_tag="ACHVC7_001951"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR2116009.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11054..11920)
                     /gene="mhpC"
                     /locus_tag="ACHVC7_001952"
     CDS             complement(11054..11920)
                     /gene="mhpC"
                     /locus_tag="ACHVC7_001952"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR2116010.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11938..12882)
                     /gene="mhpB"
                     /locus_tag="ACHVC7_001953"
     CDS             complement(11938..12882)
                     /gene="mhpB"
                     /locus_tag="ACHVC7_001953"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR2116011.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12884..14548)
                     /locus_tag="ACHVC7_001954"
     CDS             complement(12884..14548)
                     /locus_tag="ACHVC7_001954"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR2116012.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14590..15423
                     /locus_tag="ACHVC7_001955"
                     /pseudo
     CDS             14590..15423
                     /locus_tag="ACHVC7_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15500..16582
                     /gene="lacI"
                     /locus_tag="ACHVC7_001956"
     CDS             15500..16582
                     /gene="lacI"
                     /locus_tag="ACHVC7_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR2116013.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16705..19779
                     /gene="lacZ"
                     /locus_tag="ACHVC7_001957"
     CDS             16705..19779
                     /gene="lacZ"
                     /locus_tag="ACHVC7_001957"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR2116014.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19831..>20095
                     /locus_tag="ACHVC7_001958"
     CDS             19831..>20095
                     /locus_tag="ACHVC7_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2116015.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4719 a         5511 c         5223 g         4642 t
ORIGIN      
        1 attgcataaa aacattaccc agtcgaacct ccgggtctga aagcccttgc ttttcagtta
       61 ttagcaacat ttaacattta taaattaaca tctggctgcg ccagagatta cgtctctgag
      121 tgcatcgttg tgcttgtagt ataataaata aaatggagtg tcattaagtg gataaggttt
      181 tagattcagc cctcctttct tcggcaaata aaagaaaggg tatccttgct attggcgcac
      241 atcctgacga tatagaatta ggctgtggcg catcgcttgc tcgtcttgcg caaaaaggaa
      301 tttatatcgc agcagtggtg atgactaccg gcaactctgg tacagatgga ataatcgatc
      361 gccatgaaga atcgcgcaac gccctaaaga tattagggtg ccaccaaact attcatctta
      421 attttgctga cacccgcgct catttacagc tcaatgatat gatttccgcc ctggaagaca
      481 tcattaaaaa tcaaattcct tctgatgttg aaatcatgcg ggtatatacc atgcatgatg
      541 ccgaccgcca tcaggatcat ctcgctgttt atcaagcttc aatggttgcc tgccgcacta
      601 ttccacaaat tctcggctac gaaaccccaa gtacctggct ttcatttatg cctcaggttt
      661 ttgaatccgt taaagaagaa tatttcacag tcaaacttgc tgcattaaaa aaacataaaa
      721 gccaggaacg acgtgattat atgcgtcatg atcgcctgcg tgcagttgca caatttcgcg
      781 ggcaacaggt caatagcgat ctgggtgaag gctttgtaat tcataaaatg attctttgag
      841 caaattgcat gaaaacctgg atatttatct gtatgtccat agcaatgttg ctatggtttt
      901 taagtacgct aagacgtaaa cccagtcaaa agaaaggctg tattgacgcc attatacctg
      961 cgtataacga aggcccgtgt ctggcgcagt cactggataa tctactgcgt aacccttatt
     1021 tttgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg
     1081 aagtcaaacg caaatggggc gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag
     1141 gtggtgcgct gatgaatggc ctcaattatg ccacctgcga ccaggttttt ttaagtgatg
     1201 ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaattaagc
     1261 gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg gggctgttac
     1321 cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc
     1381 tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg aatgttccgt actgatgtat
     1441 tgcgtaagtt cggtttctcg gatcgtacta aagtcgaaga ccttgatctc acctggacat
     1501 tagtggcaaa cggctaccgt attcggcagg cgaatcgctg catcgtatac ccacaggaat
     1561 gcaacagtcc gcgtgaggag tggcgtcgct ggcggcgttg gattgtgggc tacgcggtct
     1621 gtatgcgcct gcataaaagg cttttattta gccgcttcgg tatcttcagt atatttccta
     1681 tgctgttggt tgtgatttat ggcgttggga tttatctcac tacctggttt aatgaattca
     1741 tcaccaccgg gccgcatggt gtggtgttgg caatgtttcc gcttatctgg atcggcgtag
     1801 tttgtgttat tggtgctttt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag
     1861 cgccgctttc cgttgtgtat gtattattag cttatgccat ctggattatc tatggactta
     1921 ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg
     1981 tggaagcgtc aaccgcttat tcccaacctt ctgtcacagg aactgaaaaa ctatctgaag
     2041 cttaatgtta aagagaaaaa ttattcagat tgcagaccag gtaattattg atgaaactgc
     2101 aggtgaagtt gttatcggcg cgaatacccg tatttgtcat ggtgccgtta ttcagggtcc
     2161 ggtagtgatt ggcgcaaact gcctgatagg taattatgcg tttattcgtc ctggcacaat
     2221 aatcagcaat ggtgtaaaaa ttggttttgc caccgaaatt aaaaatgcgg ttattgaagc
     2281 ggaagcaacg attggtccgc aatgttatat tgccgactcg gtagttgcaa accaggcata
     2341 tttgggcgca caagtacgta ccagtaatca tcgtctggat gaacagcctg tgtctgttcg
     2401 aactccagag ggaattatcg ctaccggatg cgataaatta ggttgttata tcgggcagcg
     2461 ttcacgcctt ggcgtacaag ttattatttt gcctgggcga attatttccc cgaacacgca
     2521 acttggcccg cgcgtgattg tagaacgtaa tttacctacc ggaacttatt cactccgaca
     2581 agaacttatc cgtacaggag attaaaatga taaaacggac gttattagcg gcggccattt
     2641 ttagcgcatt gcccgcttat gccgggttaa cttccattac cgcgggctac gattttaccg
     2701 attattctgg cgatcatggc aaccgtaatt tagcgtatgc tgaactggtg gcgaaagttg
     2761 aaaacgcaac gctgcttttt aatctttccc aggggcgtcg tgattatgaa actgaacatt
     2821 tcaatgccac tcgcggtcag ggtgcggtct ggtataaatg gaataactgg ctgacaaccc
     2881 gaacgggtat tgcctttgcg gataatacgc cggtctttgc ccgccaggat tttcgtcagg
     2941 atattaacct ggccctgttg ccaaaaacgc ttttcacgac cggttatcgc tacactaaat
     3001 attacgatga tgtcgaagtc gatgcctggc aaggcggcgt atcactctat actggcccgg
     3061 tgatcaccag ctaccgctat acccattatg actccagcga tgcaggtggt agttatagca
     3121 atatgatttc cgtgcgtctg aatgacccgc gcggcactgg ttatacgcaa ctatggctaa
     3181 gccgcggaac aggcgcttac acctatgact ggacgccaga aacacgctac ggcagcatga
     3241 agagcgtcag tttgcaacgt attcaaccgc tgactgagca acttaatctc gggctgacgg
     3301 caggtaaagt gtggtacgac accccaaccg atgattataa cggtctgcaa cttgcagccc
     3361 atctgacctg gaaattctga ttgcttctgc cgcccgctat ccggggcggc cttccctgcc
     3421 gattagcccc actccctttc ctcgttgttt tccgaccaca ttcaccggat aaattttatt
     3481 ctccagcgtt atatactata gggaggtatg cattgacata tagcatacct ccctatagta
     3541 tattgcgtgc agatgatgag gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc
     3601 cttactcgag ttcgtcgtat tcgggggcag attgatgctc tggaacggtc gctggagggt
     3661 gatgccgaat gccgtgccat actccaacag atcgctgccg ttcggggcgc ggctaacggg
     3721 ctgatggcag aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa tgactgctac
     3781 agccgcgaag tcagccaatc cgttgacgac actattgaac tggttcgagc ctatcttaaa
     3841 tagctgaatc tattaccata ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc
     3901 atttgctccc ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg
     3961 tgaagtgctg attaaagtca cccataccgg cgtttgccat accgacgcat ttaccctctc
     4021 cggggatgac ccggaaggtg tattcccggt ggttctcggt cacgaagggg ccggcgttgt
     4081 ggttgaagtc ggtgaaggcg taaccagcgt caaacctggc gaccatgtga tcccgcttta
     4141 caccgcggag tgcggcgagt gtgagttctg tcgttctggc aaaactaacc tctgtgttgc
     4201 ggttcgcgaa acccagggta aaggcttgat gccagacggc accacccgtt tttcttacaa
     4261 cgggcagccg ctttatcact acatggggtg ctctacattc agtgaataca ccgtggtcgc
     4321 ggaagtgtct ctggccaaaa ttaatccaga agcaaaccat gaacacgtct gcctgctggg
     4381 ctgtggcgtg accaccggta ttggcgcggt gcacaacaca gctaaagtcc agccaggtga
     4441 ttctgttgcc gtgtttggtc ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca
     4501 ggcgaaagcg ggacggatta tcgctatcga taccaacccg aagaaattcg atctggcgcg
     4561 tcgcttcggt gctaccgact gcattaaccc gaatgactac gacaaaccga ttaaagatgt
     4621 cctgctggat atcaacaaat ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa
     4681 cgtgatgcgt gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg
     4741 ggtggcaggt tctggtcagg aaatctccac ccgtccattc cagttggtca ctggtcgcgt
     4801 atggaaaggt tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga
     4861 agatgcgatg aaaggtgata tcgatctgga accgtttgtc acgcatacca tgagccttga
     4921 tgaaattaat gacgccttcg acctgatgca tgaaggcaaa tccattcgaa ccgtaattcg
     4981 ttactgattt cccgcaggta taccccgtcc acttcagacg gggttcttaa tactctcccc
     5041 gggcagccgt ccgggggatt aaccgtgaga taatgactga tggaactcat tgaaaaacat
     5101 gccagctttg gcggctggca aaatgtgtat cggcattatt cccaatcact gaaatgtgaa
     5161 atgaatgtcg gcgtctatct cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac
     5221 tggctttcag gcctgacctg caacgagcag aatttcatta ctaaatcggg gatgcagcgt
     5281 tacgcggctg agcacaacat tattgttgtt gcgccggaca ccagtccgcg aggcagtcat
     5341 gtcgcagatg ccgaccgtta cgatctcggg caaggtgccg ggttttacct gaacgcaacg
     5401 caagcgccgt ggaatgaaca ttacaaaatg tatgactata tccgcaacga gctgccggat
     5461 ttagtgatgc aacattttcc ggcaacgacc agaaagtcta tctctggtca ttctatgggc
     5521 gggctgggcg cgctggtgct ggcgttacgt aacccagatg aatatgtcag cgtctcggcg
     5581 ttttcgccca ttgtctcccc atcgcaagta ccgtggggac agcaagcctt tgccgcatat
     5641 cttggtgaaa ataaagatgc ctggttggat tacgatccgg tgagtcttat ttcacaaggt
     5701 caacgcgttg cggaaatcat ggttgatcag gggttgagtg atgattttta cgcggaacag
     5761 ctgcggactc caaatcttga aaagatctgc caggagatga atatcaagac gttaatccgt
     5821 tatcacgagg gttacgacca cagctattat tttgtctcca gttttattgg cgagcatatt
     5881 gcctaccacg ccaataaact gaatatgcgt tgataatagt gcacgactgc cggatgcggc
     5941 gtgaacgcct tatccggcct acacttcgcc cgtaaaccgt aggcctgata agacgcgcaa
     6001 agcgtcgcat caggcatggc gcacgactgc cggatgcggc gtgaacgcct tatccggtct
     6061 acacttcgcc cataaaccgt aggcctgata agacgcgcat agcgtcgcat caggcatctg
     6121 cgcacgactt atctatgttt accacatcaa atcatcaggc actttgaagt cggcatacgg
     6181 atcgtcttca tcctgctctt cggcactgag cgcgctgtgc aacacaatac tgctggcatc
     6241 acgctgagca attttatcgg caacactggc gggaataatg gtgtattcac cttcgctatt
     6301 gttatcgacc aacaagcggg caatcgccag acgaccatta atcaactgcg cctgtgtgag
     6361 cttatcgaca aaaatcttct taatcagatt gccgtcagtg aagttaaaac caatatcgcc
     6421 attggcaatg gtgattcggt tcatttcaat gagctgcttc acctgggctt tatattcttt
     6481 cgccaacgcc gcttgttttt gctgttcgct aagctgttta tcacgctcaa gctgtgcctt
     6541 tttattttct tctaccgcag cccgagcttc acgcgcctga acgcgcgatt ttttcgccgt
     6601 tctctcgacc ttcgccgctt ttttgctggt gactaatcct gctttgagca actgctcttg
     6661 taaggtaagt tttgccattt ttgtttccaa acccgctgaa taatttttag cgagtatacc
     6721 tgtaaacgcc gtgggtgtat tgggggcaag tccctttcgc ccctgcttaa taaggcgcgt
     6781 caggcatcgg cacacggctg cattcttgat ctccgactca tcaaaataaa caccgccaac
     6841 ccagcaacaa gaatacccgg tgcagaagcg gccattacgc cgaccgtgcc agtgcctaat
     6901 gccagcattt tcccggccag taacggaccg ctcatagccc ccagacgccc taccgccacg
     6961 gctgttccca cacctgttgc gcggatctgc gaactgtaaa acaacggtgc cagggcatac
     7021 aaaacgcttt gcccacctgt cgcaaacaac cccgcgacaa atcccgccag caacataccg
     7081 ttaaatgacg acaccgttcc aagcgccagc agcgaagcta acatgccgct ataaatcagt
     7141 agcgacatgg ttactggacg cagcttatcc atcaatgcgc ccaacattaa cgtcccgctt
     7201 gccgccccca tttgcagggc aaacatcacc cctgccgcct gcgatggctg gaatccttgc
     7261 tccaccaaaa gtagcggtag ccagttgatc aacatataaa ccaccagcag agtgaagaaa
     7321 taacacaacc acagcagcag cgtcgcggtt gccgtttctg gcgcaaataa ggcacgcagt
     7381 ggtggcgcag cctgtttttc gccagcgaaa accgctgact ccggcagcca gcgcattaat
     7441 agcggcacca gaatcaacgg caccacacca cctacccaaa acaccgtttg ccatgctaag
     7501 tttgcccccg cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac accgcaatac
     7561 atcaggctca ctgccgtccc acgaaaacgt ggacccgcgg cttcagatgt cagggcgata
     7621 agattcggca acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa gaccagtgag
     7681 gggaaatccc aggcaatcgc cgttgccagt gagaacaaac caaacagcgc aactgagcca
     7741 atcaaaatac gcttacgacc ataacggtct gccagcatcc cgccaaccaa cgcgccgggt
     7801 agcaaaccga gtattccggc gctaaatatc cagcccattt gcattttatc gagtgcgaaa
     7861 gcctgggcga tgccacccgc cgcaatgcca gccgcctgaa gatccagccc ttccatcaga
     7921 gcgaccaaaa aacaaagccc gatggtcagc atcaggcggg atgaagatga tgaaggggta
     7981 cgagtcgaca taagatgaac ctcattgcga gtgcaggtga atcgcgactg atacattgcc
     8041 agccgcgtaa tcagaactat ttaatgactg cctacgggcg aaatgtagcg aaatgtaggc
     8101 cggataaggc gttcacgccg catccggcag tcgtgcgcag atgcctgatg cgacgctaac
     8161 gcgtcttatc agacctacaa attctcacgc catccgtagg ccggacaagg cgttcacgcc
     8221 gcatccggct gtcgtgctgg gatgggcata caaaatgcac gttatcggca gtcatcatta
     8281 tttgttgttg cgcagatcca gcgccacgtc gacgatcata tcctcctggc cgccaaccat
     8341 ccggcgtttg cccagctcaa cgagaatatc caccgcactt aagccataac gcgccgccgc
     8401 cgtttcacag tgacgcagga agctcgagta aacaccagcg tatcccagcg ccagcgtttc
     8461 gcgatcgact cgtaccggac gatcctgcaa cggacgcacc aggtcgtcgg cggcatccat
     8521 taacgcatag agatcggtcc catgctgcca gcccagttta tccgcggcgg caataaacac
     8581 ttccagcggt gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt cgattcggtc
     8641 gcagccctct tccaccgccg cgatagagtt cgccacgcca agactcaggt tatggtgagc
     8701 gtgcatgccg gtttgcgttt ctggtttcaa cactgctttc agggcgcgga aacggtcacg
     8761 gatatcgctc atgttcatcg caccgccaga atccaccaca taaatacagg tcgcaccgta
     8821 gccttccatc agctttgcct gcttggcgag attctccggc gtggtcatat ggctcatcat
     8881 cagaaaacca acggtgtcca ttccgagctc gcgggcatac tgaatatgct gggcggaaac
     8941 atcagcttcg gtacagtgcg ttgccacacg aaccacccgc gcgccagcct gccaggcatt
     9001 tttcagatcg tgaatagtgc cgattcctgg cagcaacaac gtcgcgattt tggcgtgcct
     9061 caccacatcc gccgccgctt caatccattc aaggtcgcta tgtgcgccga aaccatagtt
     9121 aaagctggaa ccttgcaaac cgtcgccgtg ggccacttca atcgaatcca cgcgggcatc
     9181 gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac gaatggcgtg
     9241 cataccgtca cgcaatgtga cgtccgagat ataaagtttt ttaccgttca tgccgcttct
     9301 cctgccttgc gcgccagtga ctgggccatt ttttccgctg tcgccagcgc actggaagtc
     9361 ataatatcga ggttgcccgc ataggcaggc agataatgcg ctgcgccttc gacttccagc
     9421 cagaccgctg ttttcagtcc ggagaattgc cccacgcccg gtaaattgac cggtttatcc
     9481 tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat aacccggtac gtaagcctgc
     9541 accgcctcag ccatttcatt gattgaggct tcgatatcat cttgtgaagc ttcgtcgctc
     9601 aatacataca ccgtgtcacg catcatcaac ggtggctctg ctgggttaag cacaataatc
     9661 gccttccctt ttgccgcgcc gcccaccact tcaatggctc gggaagtggt ttccgtaaat
     9721 tcatcgatat tggcacgcgt gccaggtccg gcagatttac tggcgataga agcgataatt
     9781 tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca ccattggaat ggtggcctgg
     9841 ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag gttaaccacc
     9901 ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat atccggtttc
     9961 gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc atcaaataca
    10021 atgtcgatat cagcaaattc aggcatgttc atcagcccga tcaccccttc atgggtggtg
    10081 gcgacgccca tacgtctggc acgcgccaga ccgtcggact gaggatcaat gccgaccatc
    10141 accgccatct ccagatgctg accgtgacgc aaaattttaa tcatcagatc ggtgccaatg
    10201 ttgccagaac cgataatggc gactttacgc ttactcatga cagacttcct tttggggctg
    10261 cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat atgggcttca aaacgatcgc
    10321 ccgcattcac cgccaccatc gggcctaatg ccccggtaag aatgatatct ccggcgcgca
    10381 gcggttcacc cagactggcc attttgcgtg ccagccagac ggcggcatta agcggatgtc
    10441 ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc atcttcatgg
    10501 cgcagttttt caggtctaac cccgccggac gttgcgccgg accgccgatg acatacaccc
    10561 cgcaggaagc gttatctgcc acggtatcga caaactgaat cgaccagtcg cgaatgcggc
    10621 tccccaccac ttccagcgcc ggaagtaccc attcaatggc gttatacagt tcgtcgaagg
    10681 tgatatcagt tgcgggtaaa tcgcggttca acaccagtgc gatctccgct tcaatgcggg
    10741 gttgcagaac acgggaaaaa ggaatgattt cgttatcgcc ataacacatg tcggcaaata
    10801 acgtcccaaa atccggttga tcaacgccca gttgttgttg cactttcgga tgcgtcaggc
    10861 ccactttacg ccctaccacg cgacgcccct gcacaacgtc atattgcaca tttatgtgct
    10921 gaatggcgta ggccgcttca gcgtgatcga taccaatcag atcgcgcagc ggcgcaatcg
    10981 cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga gtatgcttcg
    11041 tcatgaccat tccttaaggg cgtgcgagga aattcagcac caattgattg aaagcgtcgg
    11101 catgttccca ctgcgcccag tgaccacagt cgcggaagat atgcagttca gaaccggcaa
    11161 tgccggacag cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg cgcccccaga
    11221 caatcagggt ttgcgctttg atttccgcca gacgtgggcc aaaatccggg aactgtttcg
    11281 gattagcttc caggctctta acgaagtttt ccaggtgatc gcggcgcgac agcatattat
    11341 tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt atcgaaaacg aagatatcca
    11401 tcatcagctt caggttttcg atagtcggct gacgataaag ctgattcagt cgcttaatac
    11461 cttcggttgg catcggcgta aacaaactca tgccgcccgt accgccgccc atcagcacca
    11521 gtttgccgac gcgctccggc cagttaaggg tgaacgccac agaactatgg ccgcccatcg
    11581 agttgcccag caggtggatt ttggcgatat ccagttgatc caccacgctt ttcaggattc
    11641 gtgcattaag atccgatcgt gaaccactat taacgatcga atcgcttttg ccccaacccg
    11701 gacaatccag caggatcacc cgatagcccg cctctaccag cggatcgata ttgcggctga
    11761 agttcgccca gccagtagca cccgggccgg aaccatgcag caggacaacg gtttcgtcgc
    11821 cttgtccgca gtcattaaaa tggatgcgca gcgttttacc cgcttcttct acattcagaa
    11881 aacggctggt ggcggcttcg gtttgtggct gataactcat catcttctcc tgcatattca
    11941 gttctctgtt ctggcgctta acgagccaaa tccggcaatc cactccggga ttgggcggta
    12001 ataacgccct tcgctacgcc agttgccaaa tgcagaaata gcggcaaaag cggcgaccca
    12061 ggttttgatt tcatgtgtcg actttccggc aatggcggaa agctcttcgt tactgacggc
    12121 atccagttcc tgtatgcgtc cctgctccag caaagtcatg aactggttat cccaaatcgg
    12181 gttgagcgga tgcagagttc tctgatcctc aacaaacttc tcagcggcgc taatcaccct
    12241 ttgctgacgc aattcgcgct cactggcggg taaatctttc ccgctcccca acagacggtc
    12301 gcgcatatgg gcatcggctt tcgccagttc gggcaccggc ggctgatggg aaagcccacc
    12361 ggaacccagg aacagcacgc gtttattgag agtgctggtg aaacgtccaa tggcttcacc
    12421 caacatgcgg gtacgctgga aaccgggcag cggcgtggcg acaccgttga tgaacacagg
    12481 cagaactggc accttatcca gcccaccgag caggaactcc agcggctggg cgaacccgtg
    12541 gtccacctgc atacagtaag aaacggcaag atcgatcccg ctcttcatga cagcatgcgc
    12601 acaggcctcc gccagctcca caggcacggg cagctctcct gccgcactgc cgaaatcacc
    12661 aattgccgtc gctccaacgc ctaaacagaa cggtggcatc acgtcataga aaaagccgtt
    12721 gtagtgatct ggcgcaaaca gcaccaccag ttcaggggag aaagccgcaa tacgctcgcg
    12781 ggcgctggca atcacgccat tgacctcatc gagtacctct tgcgccgggt cgacataccc
    12841 caccaacggc gagtgggaaa gacagtgaag ataagcgtgc atatcaggct accttttcga
    12901 cagaaacgtc ggcatcaggg cgggtcagcg tcatcaccga cgccagttta ttcagcgtat
    12961 tgcccagggt ttgcggaatg gcggtggcgg caacaaagcg atccgggcgc atcaccacca
    13021 gcgaagcatt gtgttgcgcg aaccagctac gcagacgacc ttgcgtatcg cccacgtgta
    13081 gcacgccgtc gtggttatcc tgtgcggtat gaatttgcac ttccggcacc acctgaatga
    13141 agcgtgtgcc caacgcgcgc cactgctgga tttgctcgtc gctcatcccc cacagtggat
    13201 tgcatcccca gccaattacc gcgaagttcg cgccgatcgc gttatcgagc agcgtcacgt
    13261 cgccgttttc cagcgtgact ttcggctgaa taaacatctt gccgaccgga gagtgcttcg
    13321 cttcgccctc acgcaccagc gcaccgccgt aatattgcgg catcggcttg aagcgcattt
    13381 cgaggaagta gcgttttact ggcggcagat aattcaacag ccaggaaacg ccgtcacgta
    13441 acgtaccctg ccagcgtttc ggcggagcca gcacgttgcc cgccgtcacg gacaggtcaa
    13501 tcatcgcttt ggcgtgatcg cgacgttctt gttgataggt atcgagcagc gcatcgcggg
    13561 ctttcccctg gataaccaac gccagtttcc aggcgaggtt aaaggcgtcg cgcataccac
    13621 tgttatagcc ctgcccctgc cacaccggca tgatgtgcgc ggcatcgccc gccagcagta
    13681 cgcgatcaat acggaaacgt tgcgccagtc gcgcgttatg ggtgtagaca cgctggcgaa
    13741 tcaattcaac attgtccgga ttaggcagca ctttgcttaa cagcttgcgc atattttgcg
    13801 gctcacgcag ctgctcttcg gtttctcccg gcatcaccat aaattcaaag cgacgtaccg
    13861 catgaggcag cgcggcagaa acatacgggc gcaccggatc gcaacacaaa tagatatgcg
    13921 gcgtacttaa cggatcgttg gcgatatcta ccacaatcca ctgatttggc gcagttttac
    13981 cttcaaacgg cacattcagg gtgcgacgaa caaaacttgc cccaccatca caggccacca
    14041 gccactgggc tttgatcgtt tcccgctgcc cttctgccgt tttcaggtgc aaggtcactt
    14101 cgtcatcttg ctggctgaag gcctccagct cgcgggaaaa caagcagcgc acattcggaa
    14161 aacgcgacaa cccttccagc atcaccgcat cgacctgtgg ctgaataaag gcgttacggc
    14221 gcggccagcc aaattcatcg gtcattggct gaatatcagc aaaacagcgg ccttttgggg
    14281 tgagaaaacg catcgcgtgc cacggtgtag tgtgcggcag aacattctcg accaggccga
    14341 ccgactgcat ggtgcgcagc gcctcgtcat caataccaat cgcacgcggg tagtcgatca
    14401 acttatcgag tttctccacc accagcacgt caatgcccat ctgaccgaga tagttcgcca
    14461 tcatcagccc aaccggaccg gcaccagcga tcgccacctg aacgctatgg ttaacagcag
    14521 gctggatgtc agggtgttgt attgccattt ctgcacaacg ttgttttaac tatagaaatg
    14581 tcaattaata tgcagaacaa tgagcagacg gaatacaaaa ccgtgcgcgg cttaacccgc
    14641 ggtctaatgt tattaaatat gttaaataaa cttgatggcg gtgccagcgt cgggctgctg
    14701 gcggaactca gcggcctgca tcgcaccact gtgcggcgac tgctggagac gctgcaggaa
    14761 gagggatatg tccgccgtag cccctccgat gacagttttc gtctgaccat caaagtgcgg
    14821 caattaagcg aaggatttcg tgacgaacag tggatttctg cactggcggc cccgctgctg
    14881 ggcgatctgt tgcgcgaagt ggtatgacca acagatgtgt ccaccctgga tgttgatgca
    14941 atggtggtac gcgaaaccac ccaccgtttc agccgcttat cctttcaccg ggcaatggtc
    15001 gggcgacgtt taccgctcct gaaaaccgcc tcgggcctga cctggctggc cttttgcccg
    15061 gaacaagaac gcaaggaatt aatcgaaatg ttagccgccc gccccggtga tgactatcaa
    15121 ctggcacgag aaccgttaaa gctgcaagcc attctggcgc gcgcgcgcaa agagggttac
    15181 ggacagaact accgcggctg ggatcaggag gagaagatcg cctctatcgc cgtaccgctg
    15241 cgcagtgaac aacgggtgat tggctgtctg aatctggtgt atatggcgag cgcaatgacc
    15301 attgaacagg cagcggaaaa gcatcttccg gcgctacaac gggtagcaaa acagatcgaa
    15361 gaaggggttg aatcgcaggc tattctggtg gccggacggc gaagcggcgt gcatttacgt
    15421 tgacaccatc gaatggcgca aaacctttcg tggtatggca tgatagcgcc cggaagagag
    15481 tcaattcagg gtggtgaatg tgaaaccagt aacgctatac gatgtcgcag agtatgccgg
    15541 tgtctcttat cagaccgttt ctcgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
    15601 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
    15661 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
    15721 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
    15781 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
    15841 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
    15901 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
    15961 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
    16021 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
    16081 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
    16141 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
    16201 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
    16261 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
    16321 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
    16381 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
    16441 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
    16501 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    16561 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    16621 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtgaaatt gtgagcggat
    16681 aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta
    16741 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    16801 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    16861 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
    16921 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
    16981 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
    17041 ccttttgttc ccgcggagaa tccgacgggt tgttactcgc tcacatttaa tattgatgaa
    17101 agctggctac aggaaggcca gacgcgaatt atttttgatg gtgttaactc ggcgtttcat
    17161 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gccgtttgcc gtctgaattt
    17221 gacctgagcg catttttacg cgccggaaaa aaccgcctcg cggtgatggt gctgcgctgg
    17281 agtgacggta gttatctgga agatcaggat atgtggcgaa tgagcggcat tttccgtgac
    17341 gtctcgttgc tgcacaaacc gagcacacaa atcagcgatt tccatgttgc cactcacttt
    17401 aatgatgatt tcagccgcgc ggtactggag gcagaagttc agatgtacgg cgagctgcgc
    17461 gatgagctgc gggtgacggt ttctttgtgg cagggtgaaa cgcaggtcgc cagcggcacc
    17521 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacgcta
    17581 cgtctgaacg tcgaaaaccc ggcgctgtgg agcgccgaaa tcccgaatct ctatcgtgcg
    17641 gtggttgaac tgcacaccga cgacggcacg ctgattgaag cagaagcctg cgatgtcggt
    17701 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt
    17761 cgcggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg
    17821 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ttgttcgcat
    17881 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct tcggcttgta cgtggtggat
    17941 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc
    18001 tggttacccg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg
    18061 agtgtgatca tctggtcgct ggggaatgag tcaggccacg gcgctaatca cgacgcactc
    18121 tatcgctgga tcaaatctgt cgatccatcc cgcccggtgc agtatgaagg cggcggagcc
    18181 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc
    18241 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc
    18301 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg cttcgctaaa
    18361 tactggcagg cgtttcgtca gtacccccgt ttacagggcg gcttcgtctg ggactgggtg
    18421 gatcagtcgc tgattaaata tgatgaaaat ggcaatccgt ggtcggctta cggcggtgat
    18481 tttggcgata caccgaacga tcgccagttc tgcatgaacg gtctggtctt tgccgaccgc
    18541 acgccgcatc cagcgctgac agaagcaaaa caccagcagc agtttttcca gttccgttta
    18601 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctt
    18661 ctgcactggt cggtggcgtt ggatggcaag ccgctggcaa gcggtgaaat gcctctggat
    18721 gttgctccac aagataaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc
    18781 ggacaactct ggcttacggt acacgtagtg caaccgaacg cgaccgcatg gtcagaagcc
    18841 ggacacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagcgt gacactcccc
    18901 gccgcgcccc acgccatccc gcaactgacc accagcgaaa cggatttttg catcgagctg
    18961 ggtaataaac gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc
    19021 gctgaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac
    19081 gacattggcg taagtgaagc gacccgcatt gacccgaacg cctgggtcga acgctggaag
    19141 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgcc
    19201 gacgcggtgc tgattacgac cgctcacgcg tggcagcatc agggaaaaac cttatttatc
    19261 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
    19321 gtagcgagcg atacaccgca tccggctcgg attggcctga cctgccagct ggcgcaggta
    19381 gcagaacggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
    19441 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc
    19501 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg
    19561 cgcggcgact tccagttcaa tatcagtcgc tacagccaac aacaactgat ggaaaccagc
    19621 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg
    19681 gggattggtg gcgacgactc ctggagcccg tcagtttcag cggaattcca gcttagcgcc
    19741 ggtagctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
    19801 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg
    19861 atgttcggtt tattcttttt cttttacttt tttatcatgg gggcctactt cccgtttttc
    19921 ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc
    19981 gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc
    20041 gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgc
//
LOCUS       DAZPLM010000065        19886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPLM010000065 DAZPLM010000000
VERSION     DAZPLM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.065"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..596)
                     /locus_tag="ACHVC7_001959"
     CDS             complement(<1..596)
                     /locus_tag="ACHVC7_001959"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR2116016.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDG"
     gene            complement(683..2980)
                     /locus_tag="ACHVC7_001960"
     CDS             complement(683..2980)
                     /locus_tag="ACHVC7_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR2116017.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(2970..3245)
                     /locus_tag="ACHVC7_001961"
     CDS             complement(2970..3245)
                     /locus_tag="ACHVC7_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR2116018.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3242..3466)
                     /locus_tag="ACHVC7_001962"
     CDS             complement(3242..3466)
                     /locus_tag="ACHVC7_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR2116019.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3469..3768)
                     /locus_tag="ACHVC7_001963"
     CDS             complement(3469..3768)
                     /locus_tag="ACHVC7_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR2116020.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3768..3992)
                     /locus_tag="ACHVC7_001964"
     CDS             complement(3768..3992)
                     /locus_tag="ACHVC7_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR2116021.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4056..4556)
                     /locus_tag="ACHVC7_001965"
     CDS             complement(4056..4556)
                     /locus_tag="ACHVC7_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR2116022.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4726..4998)
                     /locus_tag="ACHVC7_001966"
     CDS             complement(4726..4998)
                     /locus_tag="ACHVC7_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR2116023.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5135..5428
                     /locus_tag="ACHVC7_001967"
     CDS             5135..5428
                     /locus_tag="ACHVC7_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2116024.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5498..6478
                     /locus_tag="ACHVC7_001968"
     CDS             5498..6478
                     /locus_tag="ACHVC7_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2116025.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6664..7164)
                     /gene="cpxP"
                     /locus_tag="ACHVC7_001969"
     CDS             complement(6664..7164)
                     /gene="cpxP"
                     /locus_tag="ACHVC7_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR2116026.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7314..8012
                     /gene="cpxR"
                     /locus_tag="ACHVC7_001970"
     CDS             7314..8012
                     /gene="cpxR"
                     /locus_tag="ACHVC7_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR2116027.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8009..9382
                     /gene="cpxA"
                     /locus_tag="ACHVC7_001971"
     CDS             8009..9382
                     /gene="cpxA"
                     /locus_tag="ACHVC7_001971"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR2116028.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9488..10162)
                     /gene="yiiM"
                     /locus_tag="ACHVC7_001972"
     CDS             complement(9488..10162)
                     /gene="yiiM"
                     /locus_tag="ACHVC7_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR2116029.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10311..11294)
                     /gene="kdgT"
                     /locus_tag="ACHVC7_001973"
     CDS             complement(10311..11294)
                     /gene="kdgT"
                     /locus_tag="ACHVC7_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR2116030.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11554..12174)
                     /gene="sodA"
                     /locus_tag="ACHVC7_001974"
     CDS             complement(11554..12174)
                     /gene="sodA"
                     /locus_tag="ACHVC7_001974"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR2116031.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12459..13493
                     /gene="rhaT"
                     /locus_tag="ACHVC7_001975"
     CDS             12459..13493
                     /gene="rhaT"
                     /locus_tag="ACHVC7_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR2116032.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13490..14338)
                     /gene="rhaR"
                     /locus_tag="ACHVC7_001976"
     CDS             complement(13490..14338)
                     /gene="rhaR"
                     /locus_tag="ACHVC7_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR2116033.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14412..15248)
                     /gene="rhaS"
                     /locus_tag="ACHVC7_001977"
     CDS             complement(14412..15248)
                     /gene="rhaS"
                     /locus_tag="ACHVC7_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR2116034.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15536..17005
                     /gene="rhaB"
                     /locus_tag="ACHVC7_001978"
     CDS             15536..17005
                     /gene="rhaB"
                     /locus_tag="ACHVC7_001978"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR2116035.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17002..18261
                     /gene="rhaA"
                     /locus_tag="ACHVC7_001979"
     CDS             17002..18261
                     /gene="rhaA"
                     /locus_tag="ACHVC7_001979"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR2116036.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18503..19327
                     /gene="rhaD"
                     /locus_tag="ACHVC7_001980"
     CDS             18503..19327
                     /gene="rhaD"
                     /locus_tag="ACHVC7_001980"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR2116037.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19337..19651
                     /gene="rhaM"
                     /locus_tag="ACHVC7_001981"
     CDS             19337..19651
                     /gene="rhaM"
                     /locus_tag="ACHVC7_001981"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR2116038.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4967 a         5131 c         4927 g         4861 t
ORIGIN      
        1 tggccatctt ctaatggagg aagatttcct attgcatctc taactgtttt caaaataaca
       61 tcgtcacgtt ttactaactc aatcgccccc aattttgagg ccaacaatac atgacgggaa
      121 cggttttgtg ggattccata ttcgacacaa tcaatcttag acgcccaaac ataatagccc
      181 aactttaaca atgaattata aaaatcatta taaactttat gtttagttac atcaggaacg
      241 ttctccattg ttacgatttc tggagatact tcgcgaatta aacgttcgaa ctcataaaga
      301 agaggccatt ttttatcatc agccttatct tttccttgag tatattttga aaaaggttgg
      361 caaggtgcgc atcctgccaa tacttttaca gacgcatcac cataaagcct cagcagttcc
      421 tcttttgtta ctttagcgat atcttgctca ataaacttag atttattgtt tctttcatag
      481 ggaaacctac attccccctc aaggtcaatg cctgcaacga catcaagccc cgcaagctgc
      541 aaaccatgag ttaatccgcc agcaccacaa aaaaggtcta tcacctttac agtcatcact
      601 cactccaaat ctatacgata ctcagtatac atccctttct acaaaatctc gaggcctaac
      661 ttataaactc atatttattg agttaacgct tcactgagtt tgactctaat actgttgaga
      721 atcccgacca ctcatcagcg agcgggtaag tgaatttttt cccgtcataa tttacggttg
      781 ccccacgcgc cagcacctca agctcccatc gctgcggcct gataccgttc tgagcaaggt
      841 caacgcggat acgggtaatt tgcattcgtt ccgaccgggt cagtctggcc gatggcgcta
      901 tttcatgcgg ttttaacggg cttccgtttc tttgctgacg gtttggtctt ctcagtccgt
      961 gttttaatgc acctctgagc gccctcacaa cctccgggtc attccattcg ataacaccgt
     1021 catctaccag attaagcact gctgcggcgt gctcagaagg tgtgggagcc ggtaacgaag
     1081 tatcaccacc agtgagcttt ccacagttat tgacaggact ccgaggcgcg gcgatgccgc
     1141 tttttaaagt caaaggctca acgaccggca ctttcggaac aatgcgccag tccgtcgttc
     1201 tggtgatatg aatatgacgc gcgccgagat gcggcgcgta aatgccgacc actctctcga
     1261 cttcttcctc gtactcgtta acttcatccg acggactacg ggcgaccctg acagtctgac
     1321 aatcgcgcgg gacatttgcc ccaccctgcg cgctgatata cagcgcaaaa tcaccactgt
     1381 ctgcggcggc gcgtgcagcc tcgacgcgct cgtcaaactc atcagcaatg ctgacgccgc
     1441 gaggcaattt gcgtagttca cggtaagccc ccattgtcgg caggccaacc gttttaaatt
     1501 gcggaatgcg ccacgttgac gcccatgcgg taacagccgc ggcagtatct ttcagcggtc
     1561 tgccgctatc gttatcgagc tgcccatcca gtgcatagcc gtcgatattt tttgagatgt
     1621 atttcgcgat ataccccgca gcaccgcccc gattaagatg ttttgcctga aaacggtttc
     1681 gcgcagctcc tctttcgtcg ccatcctctt tgagcgcgta gcgacgcatg atttcgataa
     1741 tctggttacg ctggcgtgga ttacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt
     1801 ggtgtggctc gacgactcgc aaaccgtaga cctgtaaatc attatccttg aatgccgtgc
     1861 gcatcaggct ccagatacgg cagagataac gctgcgcatc ctttggatta aatgcctcat
     1921 cgttccagcc gtgatttagc tggacggttt tactttcgcc ttttccaacc tgacgtgtcg
     1981 ggtgatactt tgacggcgcg gtcagcgtga taaacatccc cacatcacct tctgcggcgg
     2041 cgtaacgctc aataccggca atggtgttca tcagctccat ccggcgaatt tcaggattag
     2101 aaatactgcc catcacctta ctgataaggt cgatgcgctc gcctgtttcc ctgttttcga
     2161 gatcacacga tttaagaaat tccagatttg cctggcggcg tgcacgcaca tcacgaatgg
     2221 catgtttact ggcataagga gaacggtctt tattgacctc cccgacagca atcagtaacg
     2281 cctcatgcca gcgcatacgc tggcctttaa gctgactaat ccaccactca tcgttaaaca
     2341 gacgggcaat ggcagaatat gcctgcctcg tggtcatctg tcctttacgg tattttttcc
     2401 agtagagagg ggaaatattg aaagcacgtg cagcgccagc aacatgacca tagaggtgag
     2461 cctgcgcctc atccgtaaac agcgattctt tttcgccatg cgcatccacc caggcatcgc
     2521 tgagttcctc atacatcatg aaaagctgcg atgagatacg ggcggcaaac tttttcagct
     2581 ccttgtcatt cattcccggc aggcgcgcat agtggtcacg ctcagccaga aacagcatcg
     2641 acgcgtcggt gttcatttca tggcgctgat tcacacgctc aatgcgcggc cataaacgac
     2701 gctgaaaagt ggatgtgagg aaataaaacc cgtgcaccgg gcttttattg cgccggatgt
     2761 agtcatagcg tgacgtaaac agcgagcgca aaaagtaagg caggcggtta atcgtggata
     2821 aaacaccttg cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc ccgacagcct
     2881 cgcgtggcgc gttccatgca taagcaccgg taaacgcctt accggtgcct gcagcaaatg
     2941 ctgacggagg gacaaaacgc ccggaggctt taacggccat atgagccaaa agcctctgaa
     3001 caacgcttgc tgagttgctc aacctgcgcg tttaaatcag caaaagactt tgcgcttccg
     3061 gtcagaatat cgtgatgcat caggccggaa acgagctggc ttaatttcgg gtaataacca
     3121 accaccgcca gccattcctg accggcgttt ttaccgcttt ccgctctctt tttctcgtgg
     3181 agaataaact ggaagctgtc actggtaacg acataacgtt cgccaatttc aatacgaata
     3241 ctcatgccgt tctccggtaa tgtttgtttt ttgcttcaaa gactgactga caggaaacac
     3301 aacgcgtggc tgacggataa gccgcacgac gggcagcagg aattggcgcg tcacactctt
     3361 cgcaaaccag cgcagaagca ccgcaatgtt ttacccttgc cgcgttaatc tggcgctcca
     3421 gtaattcagc ctgttgttcc tgaataaaat ctatgttgtc cggcattatc agctcctttt
     3481 atcgttaagt ttcctggata catcagcgca ataactggca agttctgtcg ttaattttgt
     3541 cagttcatcc actgaggaaa tttgcttgtg gaatacagcg cgtttaacaa gtaaattgac
     3601 cacatcagac aggaggttta attcattctg ataaatcgcg ataacagatt cagtgatgtc
     3661 gcgtttttct ttatcaagac aaagttgaat aagagacaag tcgccatttt tcataacggc
     3721 gatttttaag gcgttattca gtaatacaac tgaatgagaa caggacatca aagcacctcc
     3781 ccgcgagaca atccgatatt gtgaaatttt tccgactcct gactgagcag ctcgactatc
     3841 tccacgcggg ataactccgc ctttgtgata tggcgaatca tggcgtcaag atgagaagaa
     3901 aagcgcgtcg cagcgtcggc ctgtgcttcg gttctggcct gttgcagcag taatgcgtat
     3961 ttaccgcact gattttcaga aactgtatgc atgactttct ccaggcaaaa agaagccccg
     4021 cacaattaag tgcgttaaaa actctggtta attatttaat gcaaatattg ctctggtttt
     4081 accgacgtca gaattgtcgg tgcatactca aacagactga atagttcacg taatgcacgg
     4141 aataaagcat cacgccagta acatgactct tcattaattc gccagtatgg ctgattgaat
     4201 tctttttcag tcaatccggc atgcataaat aaagtacgac gctgactgac tgttaaaaaa
     4261 ctaatatatg catactcact tgcgccaacc tgacggcgtt ttgagaatgc cccacgcaat
     4321 tcatcaattg cacaaaccag ccgttcacgt tcgacgtcgt tcatttcttc aaaacgcatc
     4381 gttgcgtgac gttgttttaa ctgcgcatga aagcaaactg ttagccgttc gcgctccatc
     4441 atctgattat aataatcaca tgtatcctgc caacgaggga cggcaagatg cttaccaatt
     4501 atccggcgca tagctgctgg ctgtttttcg acgagattga gcgtcatcac tgtcatttcc
     4561 agaccctccg gcttttcaga aaggtcagag ccttttttaa cggactctgt tttttggtgc
     4621 ggataatgat tcccttgcga cccttcccgt gggtgatggt gaagtcaatc gccctagggc
     4681 tttcgttacg caataactga gcaatacaac gcggctcatt cataatcaca accccatcca
     4741 caaaagccat gcatcacgct gttcaaccgg tcggttataa aacgcctcac gtacagcgcg
     4801 attaaactca ggaatgaaaa cccatttttc accggcacga gccttcggtt tgcaaggatc
     4861 acgcaattca ataattggta atttatttgc cttcaccatt tcactgacgg ctgtctttgg
     4921 cttccctaat aaatcagcaa atttatccac atgaaccgca tcaagcggat actgaatcac
     4981 ataattttca gcgtccatat atggtaccct cataggatcc agccctttct aaaccactca
     5041 aaaccgttta gacgctggtt tattctcaaa tcaatggaac ctatataggt tccagttttg
     5101 agggaattta gtccctatat aggcaccatg tcaaatgaaa ttaagcgaaa agattaaggc
     5161 cttgcgtgag gctgaagggc taagccaatc aaaattctgt gaaatcatag agttaccgct
     5221 aagcacactt aaaaaatatg aaggaggaaa ctttgaaccc ggtggcacag ctttgctaaa
     5281 aatcactatg catcccacat tccaaaaata tgctctatgg cttatgacag ataaaaccgc
     5341 gccggacgca ggacaaatcg caccggctct cgcgcacatt gggccagagt caacagagtc
     5401 caaccactcc gcgaaaagga ttggctaact ctatataaag attacatttt caccatttgc
     5461 taccaagatg gtgaatacag cgccggaggg ctttcttatg gcaattaaga agctcgatga
     5521 tggtcgctat gaagtggaca ttagacctcg cggtcgcgac ggaaaacgca tccgcaggaa
     5581 attcgaaaga aaagctgaag cactagcatt tgagcgatac acaatcgcca atgccagtca
     5641 gaaagaatgg ggaggccagc gagcagaccg ccggactttg agtgagttgc tggacatctg
     5701 gtggaaatat cacgggcaaa accacgagca tggaacaaaa gagtttaatc atctactcaa
     5761 aaccatcagc ggcataggtg atataccagt gagcaggatg agcaaaaggg ctttgatgga
     5821 ttatcgttcc atgcgactac gtgatggtat cagtgccgca acgataaacc gtgacatgta
     5881 cagattatcc ggcatgttca caaaattaat tcaattggat gaattttccg ggcaacaccc
     5941 aattcacgga ctgccgccac tggcggaggc caaccctgaa atgacgttcc tggaaaaagc
     6001 agaaatcgaa aaactgttaa atgttttggc tggtgatgac ttacttgtcg cgcttttatg
     6061 tctgagcact ggaggaagat ggacgaaagt tgccacgcta aaaccagcac agattacaaa
     6121 ttgcagggtt accttcctga aaaccaaaaa cggtaaaaag cgaaccgtgc cgatttctga
     6181 ggaactggag aaaaaagtta aagaggaggc cagcgccaaa ttattcaaag ttgattatga
     6241 gaaattttgc gggattttac gcagagtgaa acctgatata ccacccaatc aggcaaccca
     6301 catcctgcgg catacattcg caagccattt catgatgaat gggggcaata taatcgcact
     6361 gcaacagatt ctgggacatg cgagcattca gcagacgatg gcctatgcgc accttgcgcc
     6421 tgactatctg caaaatgccg tcgctctgaa tccactaaaa ggcggagtga cgttataaat
     6481 ttccccctct gagtgtccac atagtgtcca cactcaagaa attttgaaac cgctccaaac
     6541 ccttacagct ttcgataagt aactgttttt acttttgatt gcatgtaagt atttgataaa
     6601 aaaaacccca catcatgtgg gggaagacag ggatggtgtc tatggcaagg aaaacagggt
     6661 ttactactgg gaacgtgagt tgctactact caatagcttc aacgatgaac ttttttgcca
     6721 ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt ttctcgttta aaaccgcttg
     6781 ctgctccggc gttaacaggc gatacatttg gttgcggact tttgccatct caacctgacg
     6841 agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt
     6901 ttctgcggtg acaaggcgat gcattgtctc cagttcgcta acattaacag gaggctgttc
     6961 gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat
     7021 gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt
     7081 atcgcctgaa ccgacttcag cagcgtggct taatgaactg actgccagcg ttgaggccat
     7141 gacggcagcg gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg
     7201 atagagagtt tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt
     7261 catggattag cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa acaatgaata
     7321 aaatcctgtt agttgatgat gaccgagagc tgacttccct attaaaggag ctgctcgaga
     7381 tggaaggctt caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg
     7441 acagcattga tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat
     7501 taaaagcact tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg
     7561 aacttgatcg cgttctcggc cttgagctgg gcgcagatga ctatctcccg aaaccgttta
     7621 atgatcgtga gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac tggagcgagc
     7681 aacagcaaaa caacgacaac ggttcaccga cactggaagt tgatgcctta gtgctgaatc
     7741 caggccgtca ggaagccagc ttcgacgggc aaacgctgga gttaaccggt actgagttta
     7801 ccctgctcta tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc
     7861 aggaagtgtt gggcaaacgc ctgacgcctt tcgaccgcgc tattgatatg cacatttcca
     7921 acctgcgtcg taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg
     7981 gtcgcggcta tctgatggtt tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc
     8041 catcttctgg ctgacgctgg cgctggtgtt gatgttggtt ttgatgttac ccaagctcga
     8101 ttcacgccag atgaccgagc ttctggatag cgaacagcgt caggggctga tgattgagca
     8161 gcatgtcgaa gcggagctgg cgaacgatcc gcccaacgat ttaatgtggt ggcggcgtct
     8221 gttccgggcg attgataagt gggcaccgcc aggacagcgt ttgttattgg tgaccaccga
     8281 aggccgcgtg atcggcgctg aacgcagcga aatgcagatc attcgtaact ttattggtca
     8341 ggccgataac gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc
     8401 gttctccgtg cgtgatggcg aagataatta ccaactttat ctgattcgtc cggccagcag
     8461 ttctcaatcc gatttcatta acttactgtt tgaccgcccg ctattactgc tgattgtcac
     8521 catgttggtc agtacgccgc tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg
     8581 taagctgaaa aacgctgccg atgaagttgc ccagggaaac ttacgccagc acccggaact
     8641 ggaagcgggg ccacaggaat tccttgccgc aggtgccagt tttaaccaga tggtcaccgc
     8701 gctggagcgc atgatgacct ctcagcagcg tctgctttct gatatctctc acgagctgcg
     8761 caccccgctg acgcgtctgc aactgggtac ggcgttactg cgccgtcgta gcggtgaaag
     8821 caaggaactc gagcgtattg aaaccgaagc gcaacgtctg gacagcatga tcaacgatct
     8881 gttggtgatg tcacgtaatc agcaaaaaaa cgcgctggtt agcgaaacca tcaaagccaa
     8941 ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa gccgagcaaa tgggcaagtc
     9001 gttgacagtt aacttcccgc ctgggccgtg gccgctgtac ggcaatccga acgccctgga
     9061 aagtgcgctg gaaaacattg ttcgtaatgc tctgcgttat tcccatacga agattgaagt
     9121 gggctttgcg gtagataaag acggtatcac cattacggtg gacgacgatg gtcctggcgt
     9181 tagcccggaa gatcgcgaac agattttccg tccgttctat cggaccgatg aagcacgcga
     9241 tcgtgaatct ggcggtacag gattggggct ggcgattgtt gaaaccgcca ttcagcagca
     9301 tcgtggctgg gtgaaggcag aagacagccc gctgggcggt ttacggctgg tgatttggtt
     9361 gccgctgtat aagcggagtt aaactccgca tttgtaggca ggataaggcg tttacgccgc
     9421 atccggcatt tgagcaggat gcctgatgcg acgctgatag cgtcttatca ggcctacact
     9481 ccgggggtta ttttccccac aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc
     9541 gcttttgcat cgtccgcgtc cagcttttcg ataacccggc ggcggagagt aaacggtgat
     9601 actgatcgtc atcaaacggc atatgccatg cgatggcggc agcctcctgc acggtcacat
     9661 cgctgacacg ggaaaccaac tccagcggcg cgtccgcaga cactttccct ggcgctatca
     9721 cgctatacaa ccaccccacc ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat
     9781 caaaatggta attgagttta tagcaaggcg agcgcggctg gctgacctga attaatgcct
     9841 ctccccagcg gaaaatatcg cccatatgaa cattactttc cgtgagaccg tcggttgaga
     9901 gattttcgcc aaacgcaggc gcaacaaaca actccgcctg ttccggaaat tcccgcaccc
     9961 agtagagata gtgctcacga ggataatgac acagcgctct gtctggcccg ccgtgaacct
    10021 ttttctccgc ctgctcgtca ccttccagcc ccagctccgt cagcatcaac tcaccatcaa
    10081 cctggatttt ggcaattgcg ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt
    10141 atacatcaac cggatatcgc atctgttccc tctccacgag aaatttcccc attagaccat
    10201 atttatttta tgtcaggcgt gctgttttcg cggtaagtat ccaggcagga actcaatagc
    10261 aaaccggacg aacgttagtt catccggctg tggggattaa ggaagcaggt ttatttcacc
    10321 gtacctaaaa tttcgatttt cgctgctctg gctttgactt tacgtgacca gatagaggta
    10381 agaattggca ccagaatcga agtcacaatg accgccgtcg ctaccagcga agttgctgcc
    10441 ggagccatcg gtttaaacgc aggcaccatt tctgcaatca gcacaggtgt cgctaccgcg
    10501 gcccctgcgg aactggaagc ggcaattccg gcagtgccat cgccaccgcc aatcaattta
    10561 tcggcgataa tcaacggaat accggtcacg ataattactg ccacacccaa caggatcccc
    10621 agcaaaccag tctgggcaat cacagtcaaa tcaatggtat tgcccagcgc gaaggcaaag
    10681 aatggaatca gcgtttgcac tgctttgctg aaaaattctc gtaattcagg gtcaagattc
    10741 cccagggcaa agcccaccag gaacggtaat actgcgccga cgaaaacatg cggttcaaac
    10801 gaggcaatcc cggcagtgcc cagaataatc atcgtcatga gcggcccgga ctccaacgac
    10861 atcaacacaa atgccccagc ttcttctttc gtaccgtact gctgcatgat ggaagcgtaa
    10921 agtccgccgt tggtcatatc catcgccgcc accagcgcca gcgttgaaag tccggcaaag
    10981 aatccaactt caacaccatg ttccggaata atgcgcgagg caattgccgc aaccacccac
    11041 gcgacggcaa ttttagttac caccagcgta ccggatttac gcagtaccgt tcccgtcgcg
    11101 cttaatttta ttgacgcccc catgcaaaaa aaccacaccg ccagaatggg caccgtaccg
    11161 gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg cccccggcga gaaggtgtgg
    11221 cacagtgcgc caaggaatag cgggacgagc atcatccccc ccgggatttt ctcaatcgag
    11281 cgttttatct gcatttccat cacctgtcgg catattttta agggctgatg cgtaattcct
    11341 caatggagca tcagtgttga tggaaacgaa gatagacatt catttaatca ataaaagtga
    11401 tcaaaaccac atcaattgaa acgctgtttt atttttatcg gatcattgtt tttattttga
    11461 aattgatcat aaaaaaacca ccgccgttgg cgatggttca ttttttaagc tgatatgcgg
    11521 cctatacgcc tcattgcagc aggcggcaaa tgattatttt ttcgccgcaa aacgtgccgc
    11581 tgcttcgtcc cagttcacca cgttccagaa ctctttaatg tagtccggac ggcggttctg
    11641 gaatttcagg tagtaagcat gttcccacac atccaggccc agaatcggga agccggaagc
    11701 gccagaaata gcttcaccca tcagcggaga atcctggtta gcagtagaaa ccaccgccag
    11761 tttatcgcct ttcagcacca gccatgccca gccggaacca aagcgggaag ctgccgcttt
    11821 ttcaaattct gctttgaagt tatcaacgga gccgaagtca cgttcgatag ccgctttcag
    11881 gtcaccctgc agggtggtgc cttttttcag acctttccag aacaggctgt ggttagcgtg
    11941 accgccagcg ttgttgcgca gtacggtttt cttgtctgct ggcagctggt ccagtttggt
    12001 gatcagctct tcaaccggca ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc
    12061 gttgtttacg taggtctgat ggtgtttggt gtggtggatt tccatggtct gcttatcgaa
    12121 gtgcggttcc agggcatcgt aagcatacgg cagggatggc agggtatagc tcatattcat
    12181 ctccagtatt gtcgggcggc cgattgttaa tgccgcgtaa gcagttggtt cattatagtt
    12241 aattaaatga tattgaaaat gattatcaat gccgtacttt tcgtaagggt atggttttgc
    12301 aggaaaatgc ccgagatgtg aagcaaatca cccacttaat gccgtgattg ccagtaaatc
    12361 gacaacggcg gcaacaggcg aaaggttaat cgacagcacg atttttacac tcatctcgtc
    12421 ggagatgtga agcgacgaaa aatgatgagg ataagaagat gagtaacgcg attacgatgg
    12481 ggatattttg gcatttgatc ggcgcggcca gtgcagcctg tttttacgct ccgttcaaaa
    12541 aagtaaaaaa atggtcatgg gaaaccatgt ggtcagtcgg tgggattgtt tcgtggatta
    12601 ttctgccgtg gaccatcagc gccctgttac taccggattt ctgggcgtat tacagctcgt
    12661 ttagtctctc tacgctgctg cctgtttttc tgttcggcgc tatgtggggg atcggtaata
    12721 tcaactatgg cctgaccatg cgttatctcg gcatgtcgat gggaattggc atcgccattg
    12781 gcattacgtt gattgtcggt acgctgatga cgccaattat caacggcaat ttcgatgtgc
    12841 tgattaacac cgaaggcgga cgcatgacgt tgctcggcgt tctggtggcg ctgattggcg
    12901 tagggattgt aactcgagcc gggcagttga aagagcgcaa gatgggcatt aaagccgaag
    12961 agttcaatct gaaaaaaggg ctggtgctgg cggtgatgtg cggcattttc tctgccggga
    13021 tgtcctttgc gatgaacgcc gcaaaaccga tgcatgaagc cgctgccgca cttggcgtcg
    13081 atccactgta tgtcgctctg ccaagctatg ttgtcatcat gggcggcggc gcgatcatta
    13141 acctcggttt ctgttttatt cgtctggcaa aagtgaagga tttgtcgcta aaagccgact
    13201 tctcgctggc aaaaccgctg atcattcaca atgtgttact ctcggcactg ggcgggttga
    13261 tgtggtatct gcaattcttt ttctatgcct ggggccacgc ccgcattccg gcgcagtatg
    13321 actacatcag ttggatgctg catatgagtt tctatgtatt gtgcggcggt atcgtcgggc
    13381 tggtgctgaa agagtggaac aatacaggac gccgtccggt aacggtgttg agcctcggtt
    13441 gtgtggtgat tattgtcgcc gctaacatcg tcggcatcgg catggcgaat taatctttct
    13501 gcgaattgag atgacgccac tggctgggcg tcatcccggt ttcccgggta aacaccaccg
    13561 aaaaatagtt actatcttca aagccacatt cggtcgaaat atcactgatt aacaggcggc
    13621 tatgctggag aagatattgc gcatgacaca ctctgacctg tcgcagatat tgattgatgg
    13681 tcattccagt ctgctggcga aattgctgac gcaaaacgcg ctcactgcac gatgcctcat
    13741 cacaaaattt atccagcgca aagggacttt tcaggctagc cgccaaccgg gtaatcagct
    13801 tatccagcaa cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa cgatggcgat
    13861 tcagcaacat caccaactgc ccgaacagca actcagccat ttcgttagca aacgacacat
    13921 gctgactact ttcatgctca agctgaccga taacctgccg cgcctgcgcc atccccacgc
    13981 tacctaagcg ccagtgtggt tgccctgcgc tggcgctaaa tcccggaatc gctccctgcc
    14041 agtcaagatt cagcttcagc cgctccgggc aataaataat attctgcaaa accagatcgt
    14101 taacggaagc gtaggagtgt ttatcgtcag catgaatata aaagagatcg ccacgggtaa
    14161 tgcgataagg acgatcgttg agtacatgca gaccattacc gcgccagaca atcaccagct
    14221 cacaaaaatc atgtgtatgt tcggcaaaga catcttgcgg ataacggtca gccacagcga
    14281 ctgcctgctg gtcgctggca aaaaaatcat ctttgagaag ttttaactga tgcgccaccg
    14341 tggctacctc ggccagagaa cgaagttgat tattcgcaat atggcgtaca aatacgttga
    14401 gaagattcgc gttattgcag aaagccatcc cgtccctggc gaatatcacg cggtgaccag
    14461 ttaaactctc ggcgaaaaag cgtcgaaaag tggttactgt cgctgaatcc acagcgatag
    14521 gcgatgtcag taacgctggc ctcgctgtgg cgtagcagat gtcgggcttt catcagtcgc
    14581 aggcggttca ggtatcgctg aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc
    14641 gtacgcagtg aaagagaaaa ttgatccgcc acggcatccc aattcacctc atcggcaaaa
    14701 tggtcctcca gccaggccag aagcaagttg agacgtgatg cgctgttttc caggttctcc
    14761 tgcaaactgc ttttacgcag caagagcagt aattgcataa acaagatctc gcgactggcg
    14821 gtcgagggta aatcattttc cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc
    14881 tgctgcaata cgctgtggtt aacgcgccag tgagacggat actgcccatc cagctcttgt
    14941 ggcagcaact gattcagccc ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca
    15001 ttggtcagac acagattatc ggtatgttca tacagatgcc gatcatgagc gcgtacgaaa
    15061 cagaccgtgc caccggtgat ggtatagggc tgcccattaa acacatgaat acccgtgcca
    15121 tgttcgacaa tcacaatttc atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg
    15181 agccggggtt ctatcgccac ggacgcgtta ccagacggaa aaaaatccac actatgtaat
    15241 acggtcatac tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt
    15301 tcttacctta aattttcgac ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc
    15361 gtgaattttc aggaaatgcg gtgagcatca catcaccaca atgaagcaaa ttgtgaacat
    15421 catcacgttc atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt
    15481 cacgaattca ggcgcttttt agactggtcg taatgaaatt cagcaggatc acattatgac
    15541 ctttcgcaat tgtgtcgccg tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg
    15601 ttacgagcgt gaatgccgca gcctgacgct gcgcgaaatc catcgtttta acaatggcct
    15661 gcatagccag aacggttatg tcacctggaa tgtggatagc ctggaaagtg ccattcgcct
    15721 tggattaaac aaggtgtgcg aggaagggat tcgtatcgat agcattggga ttgatacctg
    15781 gggcgtggac tttgtgctgc tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta
    15841 tcgcgatagc cgctccaatg gcctaatggc gcaggcacag caacaactcg gcaaacgcga
    15901 tatttatcaa cgtagcggca tccagtttct gcccttcaat acgctttatc aactgcgtgc
    15961 gctgacggag caacaacctg aacttattcc acacattgct cacgctctgc tgatgccgga
    16021 ttacttcagc tatcgcctga ccggcaagat gaactgggag tacaccaacg ccacgaccac
    16081 gcaactggtc aatatcaata gcgacgactg ggacgagtcg ctactggcgt ggagcggggc
    16141 taacaaagcc tggtttggtc gcccgacgca tccgggtaat gtcattgggc actggatttg
    16201 cccgcagggt aatgagattc cggtggtcgc cgttgccagc catgataccg ccagcgcggt
    16261 tatcgcctcg ccgttaaacg gttcacgcgc tgcttatctc tcttctggca cctggtcatt
    16321 gatgggcttc gaaagccaga cgccatttac caatgacacg gcgctggcag ccaacataac
    16381 caatgaaggc ggggcggaag gtcgctatcg ggtgctgaaa aatattatgg gcttatggct
    16441 gcttcagcga gtgcttcagg agcggcaaat caacgatctc ccggcgctta tcgccgcgac
    16501 acaggcactt ccggcctgcc gcttcatcat caatcccaat gacgatcgct ttattaatcc
    16561 tgaggcgatg tgcagcgaaa ttcaggctgc gtgtcgggaa atggcgcaac cgctcccgga
    16621 aagtgatgct gaactggcgc gctgcatttt cgacagtctg gcgctgctgt atgccgatgt
    16681 gttgcatgag ctggcgcagc tacgcggtga agatttctcg caactgcata ttgtcggcgg
    16741 cggctgccag aacacgctgc tcaaccagct atgtgccgat gcctgcggta ttcgggtgat
    16801 cgccgggcct gttgaagcct cgacgctcgg caatatcggc atccagttaa tgacgctgga
    16861 tgaactcaac aatgtggatg atttccgtca ggtcgtcagc accaccgcga atctgaccac
    16921 ctttacccct aatcctgaca gtgaaattgc ccactatgtg gcgcagcttc actcgacacg
    16981 acagacaaag gagctttgcg catgaccact caactggaac aggcctggga gctggcgaaa
    17041 cagcgtttcg cggcggtggg gattgatgtc gaggaggcgc tgcgccaact tgatcgttta
    17101 cccgtttcaa tgcactgctg gcagggcgat gatgtttccg gttttgaaaa cccggaaggt
    17161 tcgctgaccg gtgggattca ggctactggt aattatccgg gcaaagcgcg taatgccagt
    17221 gagctacgag ccgatctgga acaggctatg cggctgattc cggggccgaa acggcttaat
    17281 ttacatgcca tctatctgga atcagacacg ccagtctcgc gcgaccagat caaacctgag
    17341 cacttcaaaa actgggttga atgggcgaaa gccaatcagc tcggtctgga ttttaacccc
    17401 tcctgtttct cgcatccgct aagcgccgat ggctttacgc tctcgcatcc cgacgacagc
    17461 attcgccagt tctggattga tcactgcaaa gccagccgtc gcgtttcggc ctattttggc
    17521 gagcaactcg gcacaccatc ggtgatgaac atctggatcc cggatggtat gaaagatatc
    17581 accgttgacc gtctcgcccc gcgtcagcgt ctgctggcag cactggatga ggtgatcagc
    17641 gagaagctgg atccggcgca ccatatcgac gccgttgaga gcaaattgtt tggcattggc
    17701 gcagagagct acacggttgg ctccaatgag ttttacatgg ggtatgccac cagccgccag
    17761 actgcgctgt gcctggacgc cgggcatttc cacccgactg aagtgatttc cgacaagatt
    17821 tccgccgcca tgctgtatgt accgcagttg ctgctgcacg tcagccgtcc ggttcgctgg
    17881 gacagcgatc acgtggtgct gctggatgat gaaacccagg cgattgccag tgagattgtt
    17941 cgtcacgatc tgttcgaccg ggtgcatatc ggcctcgact tctttgatgc ctctatcaac
    18001 cgcattgccg cgtgggtcat tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg
    18061 ctggaaccta ccgctgagct gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg
    18121 gcgctactgg aagaacagaa atcgttgccg tggcaggcgg tctgggaaat gtattgccaa
    18181 cgtcacgata cgccagcagg tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg
    18241 attttgagcc agcgtgggta aacactgccg gatgcggcgc gagcgcctta tccggcctac
    18301 aggtcgaatg accatgcgtg ctgccaccct caccccggcc ctctccctga aagggcgagg
    18361 gagaaaaaca tagcctcgtg cggcaacgga gctgaacacc gctccggaca gtcccctcgc
    18421 ccctacgggg agagggttag ggtgagggga acaagccgca ccatgtctaa acaaggcaac
    18481 ctaatttaca ggaacacaga acatgcaaaa cattacccag tcctggtttg tccagggaat
    18541 gatcaaagcc accaccgatg cctggctgaa aggctgggac gagcgcaatg gtggtaacct
    18601 gacgctgcgc ctggatgacg ccgatatcgc gccttatcac ggcaactttc atgcacaacc
    18661 gcgctatatt cccctcagcc aacccatgcc tttactggca aatacgccgt ttattgtcac
    18721 cggctccggc aaattcttcc gtaacgtcca gcttgatcct gcagctaact taggcgtcgt
    18781 aaaagtcgac agcgacggcg cgggctacca tattctctgg gggttaacca acgaagccgt
    18841 tccaacttcc gaacttccgg ctcacttcct ttcccactgc gagcgcatta aagccaccaa
    18901 cggtaaagat cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt
    18961 acttgaaaac gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgagtgtct
    19021 ggtggtattc ccggatggcg ttggcattct gccgtggatg gtgcccggca cggacgaaat
    19081 cggccaggcg accgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg
    19141 cgtcttcggc agcgggtcga cgttggatga aacattcggt ttaatcgaca ccgcagaaaa
    19201 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
    19261 tgaagagttg atagcgctcg gcaagcgctt cggcgttaca ccactagcta gtgcgctggc
    19321 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca
    19381 cgaagagtat cagcgtcggc ataaccccat ctggccggaa ctggaagcag tgttgaaatc
    19441 tcacggtgcg cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccac
    19501 ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agcactgatg tttgccaacg
    19561 ttggtggaaa tatatgaccg atgttatgcc cgctaacccg gataacagcc cggtgagtag
    19621 cgagctgcaa gaagtgtttt acctgccgta attgcccaat gtgccagaga tgcctccggc
    19681 acaatatttt aaatttcgat ttgaaaaatt aaacatatta catctcccgg taagttatat
    19741 ttccctgata cattgtgagt aaatcacaaa aataatgaat aacccattaa tgattcatgt
    19801 ggtttattta aataaccatt atgtgcatta ctccgcaaat ctgacctttc actctgtctc
    19861 aaattgtcat tgtttaccga catatc
//
LOCUS       DAZPLM010000066        19154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPLM010000066 DAZPLM010000000
VERSION     DAZPLM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.066"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(185..1834)
                     /gene="pgi"
                     /locus_tag="ACHVC7_001982"
     CDS             complement(185..1834)
                     /gene="pgi"
                     /locus_tag="ACHVC7_001982"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR2116039.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2071..2267
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2359..3708
                     /gene="lysC"
                     /locus_tag="ACHVC7_001983"
     CDS             2359..3708
                     /gene="lysC"
                     /locus_tag="ACHVC7_001983"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR2116040.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3960..4232
                     /locus_tag="ACHVC7_001984"
     CDS             3960..4232
                     /locus_tag="ACHVC7_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR2116041.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4365..5237)
                     /gene="rluF"
                     /locus_tag="ACHVC7_001985"
     CDS             complement(4365..5237)
                     /gene="rluF"
                     /locus_tag="ACHVC7_001985"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR2116042.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5585..6422
                     /locus_tag="ACHVC7_001986"
                     /pseudo
     CDS             5585..6422
                     /locus_tag="ACHVC7_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6492..7298
                     /locus_tag="ACHVC7_001987"
     CDS             6492..7298
                     /locus_tag="ACHVC7_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2116043.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7308..7715
                     /locus_tag="ACHVC7_001988"
     CDS             7308..7715
                     /locus_tag="ACHVC7_001988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR2116044.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7715..8209
                     /locus_tag="ACHVC7_001989"
                     /pseudo
     CDS             7715..8209
                     /locus_tag="ACHVC7_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8275..9072
                     /locus_tag="ACHVC7_001990"
     CDS             8275..9072
                     /locus_tag="ACHVC7_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR2116045.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9083..9907
                     /locus_tag="ACHVC7_001991"
     CDS             9083..9907
                     /locus_tag="ACHVC7_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR2116046.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9959..11187
                     /gene="sorE"
                     /locus_tag="ACHVC7_001992"
                     /pseudo
     CDS             9959..11187
                     /gene="sorE"
                     /locus_tag="ACHVC7_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11570..12259
                     /gene="pepE"
                     /locus_tag="ACHVC7_001993"
     CDS             11570..12259
                     /gene="pepE"
                     /locus_tag="ACHVC7_001993"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR2116047.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12350..13981)
                     /locus_tag="ACHVC7_001994"
     CDS             complement(12350..13981)
                     /locus_tag="ACHVC7_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR2116048.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14201..17884)
                     /gene="metH"
                     /locus_tag="ACHVC7_001995"
     CDS             complement(14201..17884)
                     /gene="metH"
                     /locus_tag="ACHVC7_001995"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR2116049.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18084..18908
                     /gene="iclR"
                     /locus_tag="ACHVC7_001996"
     CDS             18084..18908
                     /gene="iclR"
                     /locus_tag="ACHVC7_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR2116050.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4511 a         5008 c         5018 g         4617 t
ORIGIN      
        1 gttaaatcac agacaagggg atttatctga taaaacaatg ccgacttatt gtcggcattg
       61 tttttaaggt tatttaactc ctcatactat gcattaatgt cgatattggc atcaggctcc
      121 ggttgccgga tgcggcgtaa acgccttatc cggcctacat atcggcatca ggcacgcatg
      181 atgattaacc gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc
      241 tgctgatttc tttgtcatct ttcagctctg gcagaatacg gttcgccagt tgtttaccca
      301 actccacgcc ccactggtcg aaggtaaaga tgttcaggat cacgccctga gtaaagattt
      361 tgtgctcata cagcgcaatc aacgcaccca ggctgaacgg agtgatttcg cgcagcagga
      421 tggagttggt cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg
      481 ccggatcttt accctgatcg cgatattcct gctcaaccac ttcgcgggat ttaccaaacg
      541 ccagcgcttc ggtctgggcg aagaagttag acagcagttt ctggtggtga tcagagagcg
      601 ggttatgggt gatagccggg gcgatgaaat cgcacggtac cattttggtt ccctggtgga
      661 tcagctggta gaacgcgtgc tgaccgttag tgcctggttc accccagata atcgggccag
      721 tctggtaatc cacaacgtta ccgttacggt caacatactt accgttggac tccatattgc
      781 cctgctggaa gtacgccgcg aaacggtgca tatactggtc atacggcaga atcgcttcag
      841 tttccgcacc aaagaaattg ttgtaccaga tgccaatcag cgccagcagg acaggcaggt
      901 ttttctcggc aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt
      961 caacgaagtt atcaaagccg atggagagaa caatcgacag gccaatcgct gaccacaaag
     1021 agtaacggcc gccgacccag tcccagaact cgaacatgtt ggcagtatca ataccaaact
     1081 cgccaacggc tttggcattg gtggaaagcg ccgcaaagtg ttttgcaacg tgtttttcat
     1141 cacctgccgc tttcaggaac cagtcacgcg cgctatgggc gttggtcata gtttcctgag
     1201 tggtgaaggt tttagatgct accaggaaca gcgtggtttc cgggtttact tttttcagca
     1261 cttcagcgat gtgagtccca tcgacgttag aaacaaagtg catgttcagg tgatttttgt
     1321 acggacgcag tgcttcggtc accatgtacg ggccgaggtc agaaccaccg atcccgatgt
     1381 tcactacgtc agtgattgct ttgccggtat aacccttcca ctcaccggag ataatcgctt
     1441 ccgagaaggt tttcatcttc tccagcaccg cgttgacttc cggcattacg tctttgccat
     1501 caaccagaat cggggtattg ctacggttac gcagcgctac gtgcagcacg gcgcggtttt
     1561 cagtgcggtt gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc agatcgcact
     1621 ctttcgccag atcctgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat
     1681 ccaccagcat ctgatcgtcg aatgttgcgg agaacttaga aaaacgatcg ccgtctttag
     1741 caaaaagatc ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc
     1801 aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa
     1861 ttgtgacttt ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt
     1921 agcgtaatgt tgagtgtaaa cccttagcgc agtgaagcat ttattagctg aactactgac
     1981 cgccaggagt ggatgaaaaa tccgcatgac cccgtcgttg acaaccgccc cgctcaccct
     2041 ttatttataa atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa
     2101 cggtgttgga ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg
     2161 aacggctggc cacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa
     2221 gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa
     2281 gtatcgctct gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc
     2341 ccctgacacg aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta
     2401 gctgattttg acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt
     2461 ttagtagtcc tctcggcttc tgctggtatc actaatctgc tggtcgcttt agcggaaggg
     2521 ctggaacctg gcgagcgatt cgaaaaactc gacgctattc gcaacatcca gtttgccatt
     2581 ctggaacgtc tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac
     2641 attactgttc tggcagaagc ggcggcgctg gcaacgtctc cggcgctgac agatgagctg
     2701 gtcagccacg gcgagctgat gtcgaccctg ctgtttgtcg aaatcctgcg cgaacgcgat
     2761 gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt
     2821 gcagagccag atgtagccgc gctggcggaa ctggccgcgc tgcagctgct cccacgcctc
     2881 aatgaaggct tagtgatcac ccagggattt atcggtagcg aaaataaagg tcgtacaacg
     2941 acgcttggcc gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca
     3001 tctcgtgttg atatctggac cgacgtcccg ggcatctaca ccaccgatcc acgcgtagtt
     3061 tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt
     3121 ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccggtc
     3181 tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa
     3241 aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac
     3301 agcctgaata tgctgcattc tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg
     3361 cataatattt cggtagactt aatcaccacg tcagaagtga gcgtggcatt aacccttgat
     3421 accaccggtt caacctccac tggcgatacg ttgctgacgc aatctctgct gatggagctt
     3481 tccgcactgt gccgggtgga agtagaagaa ggtctggcgc tggtcgcgtt gattggcaat
     3541 gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac
     3601 attcgcatga tttgttacgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa
     3661 gatgccgagc aggtggtgca aaaactgcat agtaatttgt ttgagtaaat actgtatggc
     3721 ctggaagcta tatttcgggc cgtattgatt ttcttgtcac tatgcttatc aataaacgag
     3781 cctgtactct gttaaccaac gtctttatcg agaataattg cctttaattt ttttatctgc
     3841 atctctaatt aattatcgaa agagataaat agttaagaga aggcaaaatg aatattatca
     3901 gttctgctcg caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccatca
     3961 tggcactccc ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa
     4021 cactgctgga tcgggcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca
     4081 gcattaagaa agagtacatc gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag
     4141 cccgccaatt gaagaaaaag caggcttata aaccggatcc tgaagcgtca tttagctggt
     4201 cagcaaatac gtcgacgcgc ggcaggcgct aatgaacaat caacgcctga gattttcact
     4261 cgtcagtgtg aacacaattt tcccctcacc ctaacccttt tcccgaaggg gcgaggggac
     4321 tgtccgagca cttttgggcc gttttcatca gtctgaggcg ttaatcagcg ccccttcttt
     4381 ttacgccccg gcgaggtaaa acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc
     4441 gttttttcca tcttaacgac tggacgtttg atgcccgctg ttttcggttt ggccttcgct
     4501 ttgggcttcg cctcggaaga ggaattttca atgagcttaa agagatcgat aagctcgtcg
     4561 tcggttaaat cgcgccattc tcccagcgga atgccgctta agctgacgtt cataatgcgc
     4621 gtacgttcca gctttttcac ttcatagccg aaatgctcgc acatgcgccg gatctgacgg
     4681 ttcaacccct gcaccagggt aatgcggaag acaaacggtg cttctttttt gactttgcac
     4741 tttttcgtca ccgtcccgag gatcggcact cccgccccca taccacgaat aaattcatcg
     4801 gtaatcggtt tatcgaccgt caccagatac tctttctcat gatcgttacc cgcacggagg
     4861 atcttattca ccagatcgcc gtgattggtg aggaaaatca gcccctggga gtctttatcc
     4921 aggcggccaa tcgggaacac gcgtttgctg tggttaacga aatcgacaat gttatcgcgc
     4981 tcgccatctt cggtggtgct gacaataccg acgggcttgt tcagggcgat aagtaccaaa
     5041 tcttcggctt cccgaggctc aatcaactga ccgtttactt tcacaatgtc gccgggtttc
     5101 acctgatcac caatggtggc tcgcttgcca ttaaggaaca cattgccttg ctcgatatag
     5161 cgatccgctt cgcggcgtga gcaaattccg ctttcgctga tgtatttatt taaacggact
     5221 gatgagtcgg gcagcataaa ttctcctgta aaacgcggaa tataccgtac ctcttagcgg
     5281 ggaaaaacag ggttatgcgg caacgtcgaa attaaatgtg atctgccacg ccttttatca
     5341 caaaagtgtc tccggaagta cgatgcaaat ttgcctggca aaataaggat aatcaacata
     5401 ttacgaatcg ctgcacacct gtgctgatga ctactacgca gagaaacaaa tgtgcaagag
     5461 aaagcgctat ttctccccat gcaaagagtg aaaaatcgct ttgaaaggcc ctcataatgg
     5521 ggttatatca gatcaagcgg tgagaaagcg tatttgcaca aatgtgcaga accaggaggc
     5581 ggcaatggaa aacagtgacg atatccgttt gattgtgaag attgcccaac tctattacga
     5641 acaggatatg acgcaggcgc aaatcgcgcg cgaactgggt atttaccgca ccaccatcag
     5701 ccgcttgctt aaacgaggcc gcgatcaggg aattgtcacc atcgccatca actatgacta
     5761 caacgaaaat ctctggctgg agcagcaact gaagcaaaag tttggcttga aagacgttgt
     5821 ggtggtgtcg ggaaatgatg aggatgaaga gactcaactg gcgatgatgg ggttacacgg
     5881 cgcgcaactg ctggatcgct tgctggagcc tggcgatatt gtcggttttt cctagggtcg
     5941 cgcggtgagc gcactggttg aaaacttgcc gcaagcgggg caatcgcggc agttaatctg
     6001 cgtgccaatt attggcggac cgtccggtaa actcgaaagc cgctatcacg taaacacatt
     6061 aacctacagc gtggcagcga agctgaaagg ggaatcgcat ctcgcggatt ttccggctct
     6121 gctggataac cccttaattc gtaatgggat catgcagtct cagcacttta aaaccatctc
     6181 tgcctactgg gataatctgg atgtcgccct ggtgggaatt ggctcaccgg ccattcgcga
     6241 cggcgctaac tggcatgcgt tttatggtgg tgaagagagt gacgacctga aagcaggcgc
     6301 ggtattccat tggcattgcc atgagtgaag aaaaatacag cggaattgtt ggtgcactgc
     6361 gtggaaaata tattaattgt ctggtaacga atagcagcac agctgaacta ttactgaaat
     6421 aaaaatcttc ccgatgttta ccgtaataaa aattacggcg ggtccctatt atctaaaatc
     6481 aggagtctgt tatgcaaacg tggttaaatt tgcaggataa aatcattatc gtcaccggcg
     6541 gcgcatccgg tattggtctg gcgattgtag aggaattatt agcacaaggc gcaaacgtac
     6601 agatggtaga tattcacggt ggcgatggtc aatatgaagt tcataaaggc tatcagtttt
     6661 ggccgaccga tatttccagc gccaaagagg taaatcatac ggtagcggaa attatccagc
     6721 gttttggtcg catcgacggt ctggtcaata acgccggggt caatttcccg cgtctgctgg
     6781 tcgatgagaa agcgcctgcc gggcagtatg aactcaacga agctgcattc gaaaaaatgg
     6841 tcaatatcaa ccaaaaaggc gtttttctga tgtcgcaggc ggtggcgcgc cagatggtca
     6901 aacaacatga tggcgtgatt gtgaatgttt cctcagaaag tggactggaa ggctcagaag
     6961 gccaaagctg ttacgccgct accaaagccg cgctcaatag cttcacgcgc tcctggtcga
     7021 aagagctggg taaacacggt atccgtgtgg tcggtatcgc gccggggatt ctggaaaaaa
     7081 ccgggctacg cacgccggaa tatgaagagg cgctggcgtg gacgcgcaat atcaccgtcg
     7141 agcagctgcg tgaaggctat accaaaaacg ccattcctat tgggcgcgcc ggaagattag
     7201 cagaagtcgc tgattttgtt tgttatctgc tgtctgaacg cgccagctac atcaccggag
     7261 taaccactaa cattgcgggc ggcaaaacgc gcggataagg aggctttatg gtcaacgcca
     7321 ttttttgtgc ccacggcaaa ttagcttgcg ccatgctgga atcggttcag atggtgtacg
     7381 gcgacgccaa tgtcgaggcg gttgaatttg taccaggcga gaacgccgga gacatcgtag
     7441 caaagctgga aaagttagta agcattcaca atcaagatga gtggctgatt gccgtggatt
     7501 tacagtgcgg cagcccgtgg aatgcggcag cgatgcttgc gatgcgcaat cctcgtctgc
     7561 gggtaatcag cggcctttcc ctgccgctgg cgctcgaact ggttgataac caggacagca
     7621 tgaatgttga tgaactgtgt gaacacctga cgcaaatcgc aaagcaaacc tgcgttgtct
     7681 ggaaacaact ggcaacggca gaggaagatt tctgatgaac attacgctcg cccgcattga
     7741 tgaccgtctg atccacggcc aggtcactac cgtctggtcg aaagtggcta acgcccagcg
     7801 cattattatc tgcaatgacg aagtttataa cgatgaagtg cgccggacat tattgcgtca
     7861 ggctgcgcca ccgggaatga aggttaacgt ggtcaatatt gaaaaagccg tcgcggttta
     7921 tcacaacccg caatattagg acgaaaccgt tttttatctt ttcactcgcc cacaggatgc
     7981 cttagcgatg gttcgccagg gggtaaaaat aggcaccctg aatattggcg gcatggcctg
     8041 gcggcccggt aaaaaacagt taaccaaagc ggtttcatta gatgatgatg atattaacgc
     8101 atttcatgag ttaaataatc ttggcgtcat aatggattta cgcgtcgtag cgtcagatcc
     8161 ctcaatcaat attatcgaca aaataaacga acagttaatc gcaaattaaa taatagcgcc
     8221 tgtgtacata tgctttaaca ggcagggatg acgacactta aaaggtgaca aatcatggaa
     8281 ataagtaccc tacaaattat tgctatattt cttttttcct gtattgccgg aatgggcagc
     8341 gtgctggatg aatttcagac tcatcgcccg ttaattgcct gtacggtgat tggtttaatt
     8401 ctcggtgatt taaaaaccgg aataatactc ggtggtacgc tggaattgat agctctcggc
     8461 tggatgaacg tcggcgcggc gcaatctccg gattctgcac tcgccagcat aatctccgcc
     8521 attctggtta tcgttggtca gcaaagcatc gccaccggaa tcgccatcgc gttgcctgtg
     8581 gctgcggcag gccaagtgct gacggtgttt gcccgtacca tcaccgtagt gttccagcac
     8641 gcggcggata aagcagcgga agaggcgcgg tttcgcactc tcgatattct gcatgtctcc
     8701 gcgcttggcg tgcaggcgct gcgcgttgct attccggcac tgattgtctc actgttcgtc
     8761 agcgccgata tggtcagcaa tatgttaaac gccattcccg aatttgtgac ccgtggactg
     8821 caaattgctg gcggttttat cgtggtggtc ggttacgcca tggtgctacg tatgatgggc
     8881 gtgaaatatt tgatgccttt ctttttcctc ggtttcctcg caggtggcta cctcgatctc
     8941 agcctgctgg cgttcggtgg cgtcggcgtg atcatggccc tggtctacat ccagctaaat
     9001 ccacagtggc gtaaagctga acctcaactc caggccaccg cctccaccgc ccttgaccaa
     9061 cttgacgatt aacggagcca tcatggaaca gagaaaaatt acacgcagcg atctggtgag
     9121 catgtttctg cgctccaacc tgcaacaggc gtcctttaac tttgaacgta ttcacggact
     9181 gggcttttgc tacgacatga tccccgccat caagcgactt tacccattaa aagaggatca
     9241 ggttgcggcg ctcaggcgac acctggtgtt cttcaatacc acgccagccg tatgtggccc
     9301 ggtcatcggc gtcactgccg ccatggaaga ggcgcgggcc aacggcgcgg aaattgatga
     9361 cggtaccatt aacggcatca aagtcggtct gatgggacca ttggcaggag ttggcgatcc
     9421 actggtctgg ggaacgctgc gcccgattac cgctgcgctc ggcgcatctc tggcactttc
     9481 tggcaacatc ctcggcccgc tgctgttttt ctttattttc aatgcggtgc gtctggcgat
     9541 gaagtggtat ggcctacagc tcggctttcg caaaggggtg aatatcgtca gcgatatggg
     9601 cgggaatgtg ctgcaaaaac tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg
     9661 cgtgctggtc accaaatgga cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc
     9721 cgccgatggc tccaccgtca tcatgaccgt gcagaacatt ctcgaccaac tttgccctgg
     9781 tttgctggcg ctcggtctga cgctactgat ggttcgtctg ctcaacaaaa aaattaaccc
     9841 ggtatggctg attttcgccc tgtttggctt agggattatc ggcaatgcgc tgggcttcct
     9901 gtcctgattc ttcgccccgg cacgactgcc ggggccatcg ctcaacatga ggtggtttat
     9961 gaaaacaaca gctctgcgtc tttatggtaa acgtgattta cgcctggaaa cctttgacct
    10021 tcctgaaatg caggaggatg aaatcctcgc gacggtggtc actgacagcc tgtgcctctc
    10081 ttcctggaaa gaggcaacct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
    10141 aacccaatca tcatcggcca cgagttttgc ggcgatattc tggccgtggg taaaaagtgg
    10201 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgaa actccctgac
    10261 cgcccggact gccccggcta ctccttcccg tgggtgggcg gcgaagccac gcatgtggtt
    10321 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
    10381 gaaggctcgc tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
    10441 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
    10501 ctcggcggca ccggaccaat gggattgttg gcgattgatt atgcgctgca tggaccagtt
    10561 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
    10621 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
    10681 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
    10741 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
    10801 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
    10861 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
    10921 aaactgattg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
    10981 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
    11041 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
    11101 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata tctgctcact
    11161 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
    11221 tcgccgcggc aacttcgcga cctgatgtgc ggctgattgc tgaagcggcc caatgtgcac
    11281 gagaaggata aaaaaacatc agcgccttaa ggggcgctca aattggtggc aaagtacaca
    11341 ttgtttatgc cggatgcgga ataaagcctt attcggccta cataatctcg aaaattcaat
    11401 atattgcagt tatctggtag gcctggtaag cgtagcgcat caggcaatgc tgtgtttgtc
    11461 atcagtctca gcgcccggtg caatacgtga catcgtgagc catgtcagta agtggttaaa
    11521 ttcgcagtgg tagcatgcct tccgtgattt cttactaacc ggagaagtaa tggaactgct
    11581 tttattgagt aactcgacgc tgccgggtaa agcctggctg gaacatgcac tgccgctaat
    11641 tgctgaacag ttgcagggtc gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca
    11701 gacctgggat gattacacag cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt
    11761 caccggtatt catagcgttg tcgatcccgt tgccgcgatt gaaaatgctg agatcgtgat
    11821 tgtcgggggc gggaatactt tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc
    11881 accaattact gacgtggtta aacgtggtgc gctgtatatt ggctggagcg caggcgctaa
    11941 ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt
    12001 cgatgcgcta aatctgttcc cgctgcaaat caacccgcac ttcaccaacg cgctgccgga
    12061 aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga
    12121 actgacgatt attggtctac cggaaggtaa ctggatcacg gtgagtaaag gtcacgctac
    12181 gctgggtggc ccgaacacca cttatgtgtt taaggccggt gaagaagcgg tgccgctgga
    12241 agcgggtcac cgtttttaag tgaattgccg gatgcatgat ccggcatctt gttcatagtg
    12301 cctgatgcga cgcttgacgc gtcttatcag gcctacagga ttcacaatat taatactcgt
    12361 cccgtccttc atcttcatcc ggctgttcca gcacactgta agccaccgaa caaaacagcg
    12421 agttcagacg ctgcatatct cccagtaagc ctaaatgtag cgaactggtt tcaatgctct
    12481 gcacgttttg ctgatgcagg cgatcgacgt gggcgtggga atagcggcga ttaagaatgc
    12541 gaaagcgatg cttgctgcgg cgcaaacgac gagcgctggt gacatcgcca gagaagaaca
    12601 ccgacattgc cagctttaaa ttactgagca attgctcata gagcgcatcc agttccttca
    12661 acccatcaag cgaaaatgcc cgccgtgctg ccagcgattt atcagcaatt tcgctgccca
    12721 tgcgctcgac gatatcggaa gcctgttcaa ggttgagcga catttcaata atctccgccc
    12781 agcgacgcga ctcttcttct gccagttcct cttttggcat tcgcgccaga tacagcttaa
    12841 tggcggtgta gagaacgttg atatcatctg ccagcttacg cagctctttc tcctgccgtg
    12901 gctcgccgtg catcacttta ttcagccctt ccatcatctg ttccatggcg tcgccaatgc
    12961 gcagggtttc gcgcgcggcg ttcgccagag caagcgtggg cgtatccagc gcgctgacat
    13021 ccagatgttt aggccgtagc tgggtatcca gttccggttc atcgcgaata atcgttttgc
    13081 aaaaccgtgc catcgggtca acaaatggca gcatgaccag gcaacgtaca aggttgtaga
    13141 agacgtggaa atagatcacc agttccgctt ttggcaatga caacttcccc attgtctctg
    13201 ccagcaaatg gacaaacggc aggataatca ggctacccac cagcttaaac agcaaactac
    13261 ccagcgcgac acggcgggct gcggcattgg cggcactgtt gttgagcatc gccagcaggc
    13321 cggaaccgag gttagcacca atcaccagac agagcgccac ggggaaggag ataatgcctg
    13381 cggcggtcag agtcgcggtc agcagtacag cagcaaggct ggagtagctg ataatggcga
    13441 acatcgcgcc aatcagcgca tccagcagaa tatcgccggt cagcgaggca aagatcacct
    13501 gaacgccgtt tgcctgggtg atcggcgtta cggcctgcac aatcaactcc agcgccagca
    13561 aaatcagccc aagaccaata ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc
    13621 cgaggaaaaa aatcacgccg ataaaaataa gtaacggtga cagccaggat aagtcgaagg
    13681 tgagaatacg cgccattagc gccgtcccga catctgcacc cagcacaatc accagagccg
    13741 gtgcgagggc taccagatcc tgagcgacga acgaggtcac cagcatggtg gtggcattac
    13801 tgctctgtac cagtgcggta acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga
    13861 cgctccggct aaggacagta cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa
    13921 caatatgagt cccccagacc agcagggcga cggcagaaag caggtgaagc agcgttaaca
    13981 cgagttcagg ttctccttat cgttatatgt tcctgaagca attgaatgac caaaggaccg
    14041 gaaacacgct gactggcatc tttactgtgc cttttgacat tcgtttttcc ggctaagatt
    14101 attccagcct gtttccagta taagtgtttc atattaataa agagacaggg cactcattga
    14161 gcgccctgtt tgttgtaagg aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca
    14221 gattcggtgc cagccagcgc tcaacatcgg agacgctcat acctttacgg cgggcatagt
    14281 cttcaacctg atcgcgctga atttgcgcca ccgcgtagta cttgctgtcc gggtggctga
    14341 aataccaacc cgaaaccgat gcaccaggcc acatggcgaa agattctgtg agtttcatgc
    14401 cagtatgttt ttccacttcc agcagctccc agatggtggc tttttccgta tgttccgggc
    14461 aggccgggta gcccggtgcc ggacggatgc cctggtagtt ttcgcggatc agttcttcgt
    14521 tgctgaggtt ctcgttcggc gcatagcccc agtagacttt acgcacacgc tcatggagat
    14581 actccgcaaa ggcttcggct aaacggtcgg caagcgcttt caccatgatt ttgttgtaat
    14641 catcgtgctg cgcttcaaag gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg
    14701 caaatgcgcc gatgtaatct gctttaccag aaagcttcgg cgcaacgaag tcagcgagac
    14761 agtagttagc gaatcccgtt ttttcggtct gttgacgcag atggtggctg acgttgatca
    14821 catgggtgcg cgtttcgtca cggtagattt caatgtcatc gcccacacgg tttgccggga
    14881 acaagcccac cacgccacgc ggattcagcg ttttctcggc gcttaattta tccagcatgt
    14941 cgttggcgtc tttaaacagc cgctgcgcct caacgcccac cacttcatct tccagaatgc
    15001 gcggatactt cccggccagc gaccaggtca taaagaacgg tgtccagtcg atgtaattac
    15061 gcagagtttc gatgctggct tcaacttcct gcacgccgag acggtgcgcc accggcggcg
    15121 tgtaagcctg ccagtcaaaa gcgaagtcgt tatcgcgcgc cgcttccagc gtgaccggtg
    15181 gtgtgcgcgg tttcttgcgc ccgtgctgaa tacgtacggt ttcatactcc ttgcgggtac
    15241 gagcgacaaa atcatcgcgc tgagtatcgg aaagcagcgc cgccaccaca ccaacggtgc
    15301 gcgaggcatt ctgcacatac accgtcgggc cgctgtagtt ctgctcaatt ttcaccgccg
    15361 tgtgcgcttt tgaggtcgtc gcgccaccga tcagtaacgg aatggtgaag ccctgacgct
    15421 ccatctcttt cgccacgtta accatctcgt ccagcgacgg cgtgataagc cccgaaagtc
    15481 caatcagatc agcattcact tctttagcgg tacggagaat tttttccgca ggcaccataa
    15541 cgccgagatc gacaatttcg tagttgttac attgcagcac cacaccaacg atatttttac
    15601 cgatgtcgtg gacgtcgccc ttcacggtgg cgatcaccat cttgccgttg gttttgccct
    15661 gctctttgct ggcttcaata aacggttcga ggtaggccac tgcctgtttc atgacgcgcg
    15721 ccgatttgac cacctgtggc aggaacattt tcccctcgcc aaacagatcg ccgaccacgt
    15781 tcatgccgtc catcaacggg ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg
    15841 cttcttcggt atcctgctcg ataaactcag taatgccttt gaccagcgag tattccagac
    15901 gtttattcac ttcccacgag cgccactccg cctgctgggc gttagcggtg tcgtcggttt
    15961 tgctgccgcg atatttctcg gcaagctcca gtaaacgttc agtgccatca tcgcgacgat
    16021 taagaatcac atcttccacc gcgtcgcgca gttcagcggg caggtcgtcg taaatcgcca
    16081 gttgcccggc gttgacgatc cccatatcca tgccattgcg aatagcgtag tagaggaaca
    16141 ccgcgtgaat ggcttcgcgc accggttcgt tgccacggaa tgagaaagaa acgttagata
    16201 cgccgccgga aatcagcgcg tgcggcagtt cgcgtttgat gtcttcgcag gcgccgataa
    16261 agtcctgcgc atagttgttg tgctcttcaa tgccggtggc gaccgcgaag atgttcgggt
    16321 cgaagatgat atcttctggc gggaagccaa cctcttcggt gaggattttg tacgcccgac
    16381 ggcaaatctc gattttccgt gcgcgggtat cggcctgtcc ctgttcgtca aaggccatta
    16441 ccaccaccgc tgcaccgtaa cgacgcaaca gtttcgcatg atggataaag gcgtcgacgc
    16501 cctctttcat tgagatagag ttaacaatgc ctttgccctg aatacacttc agcccctttt
    16561 cgatgacctc ccattttgag gagtcgatca taatcggcac gcgagcgata tccggttcac
    16621 cggcaatcag attgagaaaa cgcaccatcg ccgcttcggc gtcgagcatc ccttcatcca
    16681 tattgatatc gataatctgc gcgccgtttt ccacctgctg gcgggccaca tccagtgcct
    16741 cgctgtattt ctcttctttg atcaggcgct tgaacttagc ggaaccggtg acgttggtgc
    16801 gttcacccac gttcacaaac aggctatctt cgccaatgtt cagcggctcc aggccggaca
    16861 aacggcaggc tacggggatt tccggcagtt tgcgcggcgc taatccttct actgcacgac
    16921 tcatcgctgc aatatgttgt ggcgtggtgc cacagcagcc gccgacgata ttgagaaagc
    16981 ccgcttccgc ccattcacgt atttgttttg ccatcgtgtc ggcgtcgaga tcgtactcac
    17041 caaaggcgtt gggtagcccg gcgttcgggt gcgcggtgac gtagcattcc gcaatccgtg
    17101 acagctcctg cacgtactgg cgcagttcat cgggccccag cgcacagttc aggccaaagg
    17161 tcagcgcttc ggcgtggcgc aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg
    17221 tgcgcccgga ggcgtcggtg atggtgccag agatcataat cggcagctca acgcccagcg
    17281 tttcaaactc cgtttttacc gcaaataccg ccgctttggc gttaagggtg tcgaaaacgg
    17341 tttcaatcag gatcagatcc gcaccacctt ccaccagcgc tttggtggac tctcgataag
    17401 ccgccaccag cccgtcaaaa gtgatattac gaaatgccgg atcgttgacg tccggagaaa
    17461 tagacgccgt gcggttggtt gggccgagaa caccggcaac gtagcgcggt ttctccggcg
    17521 tgcgcgcggt ccactcgtca gcacaggcgc gcgccagttt cgccgccgca aagttgattt
    17581 ccgccgacag ggattccatc tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt
    17641 tggtttcgat gatatccgcg cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt
    17701 ccggtttact gagtaccagc aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa
    17761 agcgttcacc acgaaaatcg gcttcgttca gtcgatagct ctggatcatg gtgcccatac
    17821 cgccgtccag caccagaata cgttcattta actgcgcacg cagttgttcc actttgctgc
    17881 tcacacttgc tcccgacacg ctcaacccag atttaataaa aattcaacaa accatactgg
    17941 cataaacgca tctgtggtaa aagcgaccac cacgcaacat gagatttgtt caacattaac
    18001 tcatcggatc agttcagcaa ctattgcatt aactaacaat aaaaatgaaa atgatttcca
    18061 cgatacagaa aaaggagact gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac
    18121 ccgccgttgc caccgcacca gcgactggac aggttcagtc tttaacgcgt ggcctgaaat
    18181 tactggagtg gattgccgaa tccaatggca gtgtggcact cacggaactg gcgcaacaag
    18241 ccgggttacc caattccacg acccaccgcc tgctaaccac gatgcaacag cagggtttcg
    18301 tgcgtcaggt tggcgaactg ggacactggg caatcggcgc acatgccttt atggtcggca
    18361 gcagctttct ccagagccgt aatttgttag cgattgttca ccctatcctg cgcaatctaa
    18421 tggaagagtc tggcgaaacg gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga
    18481 ttattatcga tcaggtacag tgtactcacc tgatgcgtat gtccgcacct atcggcggta
    18541 aattgccgat gcacgcttcc ggtgcaggta aagccttttt agcccaactg agcgaagaac
    18601 aggtgacgaa gctgctgcac cgcaaagggt tacatgccta tacccacgca acgctggtgt
    18661 ctcctgtgca tttaaaagaa gatctcgccc aaacgcgcaa acggggctat tcatttgacg
    18721 atgaggaaca tgcactgggg ctacgttgcc ttgcagcgtg tattttcgat gagcaccgtg
    18781 aaccgtttgc cgcaatttct atttccggac cgatttcacg tattaccgat gaccgcgtga
    18841 ccgagtttgg cgcgatggtg attaaagcgg cgaaggaagt gacgctggcg tacggtggaa
    18901 tgcgctgact ttttctggcg ggcagaggca atattctgcc catcatacct gagtggcaat
    18961 agaataaggg tgtctgttaa tcgcattgac gccaaaataa cttaatgtca tacacttcac
    19021 acacttttat taactcagca ttatttttaa acatcaaacc acttaattat taacaaaatc
    19081 gcaagatctc attccaccga aacttttacg gctgcattat gagattgagc gatttatgat
    19141 atttctttag ttgt
//
LOCUS       DAZPLM010000067        18846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPLM010000067 DAZPLM010000000
VERSION     DAZPLM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.067"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHVC7_001997"
     CDS             complement(<1..103)
                     /locus_tag="ACHVC7_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116051.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHVC7_001998"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHVC7_001998"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR2116052.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHVC7_001999"
     CDS             complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHVC7_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR2116053.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHVC7_002000"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHVC7_002000"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR2116054.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHVC7_002001"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHVC7_002001"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR2116055.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHVC7_002002"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHVC7_002002"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR2116056.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHVC7_002003"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHVC7_002003"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR2116057.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHVC7_002004"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHVC7_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR2116058.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="ACHVC7_002005"
     CDS             9873..10451
                     /locus_tag="ACHVC7_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2116059.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="ACHVC7_002006"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="ACHVC7_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR2116060.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12433..13380
                     /gene="cyoA"
                     /locus_tag="ACHVC7_002007"
     CDS             12433..13380
                     /gene="cyoA"
                     /locus_tag="ACHVC7_002007"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR2116061.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13402..15393
                     /gene="cyoB"
                     /locus_tag="ACHVC7_002008"
     CDS             13402..15393
                     /gene="cyoB"
                     /locus_tag="ACHVC7_002008"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR2116062.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15383..15997
                     /locus_tag="ACHVC7_002009"
     CDS             15383..15997
                     /locus_tag="ACHVC7_002009"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR2116063.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15997..16326
                     /locus_tag="ACHVC7_002010"
     CDS             15997..16326
                     /locus_tag="ACHVC7_002010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR2116064.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16338..17228
                     /gene="cyoE"
                     /locus_tag="ACHVC7_002011"
     CDS             16338..17228
                     /gene="cyoE"
                     /locus_tag="ACHVC7_002011"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR2116065.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17413..17724
                     /locus_tag="ACHVC7_002012"
     CDS             17413..17724
                     /locus_tag="ACHVC7_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR2116066.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17708..18250
                     /locus_tag="ACHVC7_002013"
     CDS             17708..18250
                     /locus_tag="ACHVC7_002013"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2116067.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18306..>18846)
                     /locus_tag="ACHVC7_002014"
     CDS             complement(18306..>18846)
                     /locus_tag="ACHVC7_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR2116068.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4960 c         4581 g         5091 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agctcgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctaa atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc ccacccaatt gtcaccataa tgataattat
    12181 ttgttaaaca attgttttat tccagattgg ttataccaat tgcccgtcca aactccttct
    12241 gcactcccct ttcgttataa cgcccttttg caacagcttc ttaaaatcaa cttgatatgt
    12301 tttgcaacat atgtaacctg acagccaaat ccaagtaaca ggaatttaat catgtttaca
    12361 gtaatgcaac ctccccgtaa aatgcccaca cactttaaac gccaccagat cccgtggaat
    12421 tgaggtcgtt aaatgagact caggaaatac aataaaagtt tgggatggtt gtcattattt
    12481 gcaggcactg tattgctcag tggctgtaat tctgcgctgt tagatcccaa aggacagatt
    12541 ggtctggagc aacgttcact gatactgacg gcatttggcc tgatgttgat tgtcgttatt
    12601 cccgcaatct tgatggctgt tggtttcgcc tggaagtatc gtgcgagcaa taaagatgct
    12661 aagtacagcc cgaactggtc acactccaat aaagtggaag ctgtggtctg gacggtacct
    12721 atcttaatca tcatcttcct tgcggtactg acctggaaaa ccactcacgc tcttgagcct
    12781 agcaagccgc tggcacacga cgagaagccc attaccatcg aagtggtttc catggactgg
    12841 aaatggttct tcatctaccc ggaacagggc attgcaaccg tgaatgaaat cgctttcccg
    12901 gcgaacactc cggtgtactt caaagtgacc tccaactccg tgatgaactc cttcttcatt
    12961 ccgcgtctgg gtagccagat ttatgccatg gccggtatgc agactcgcct gcatctgatc
    13021 gccaacgaac ccggtactta tgacggtatc tccgccagct acagcgggcc gggcttctca
    13081 ggcatgaagt tcaaagctat tgcaacaccg gatcgcgccg aattcgacca atgggtcgca
    13141 aaagcgaaac agtcgccgaa caccatgtct gacatggctg cgttcgaaaa actggccgcg
    13201 cctagcgaat acaaccaggt ggaatatttc tccaacgtga aaccagactt gtttgccgat
    13261 gtgattaaca agtttatggc tcacggtaag agcatggaca tgacccagcc agaaggtgag
    13321 cacagtgcac acgaaggtat ggaaggcatg gacatgagcc acgcggaatc cgcccattaa
    13381 aggggttgag gaagaataaa gatgttcgga aaattatcac ttgatgcagt cccgttccat
    13441 gaacctatcg tcatggttac gatcgctggc attattttgg gaggtctggc gctcgttggc
    13501 ctgatcactt acttcggtaa gtggacctac ctgtggaaag agtggctgac ctccgtcgac
    13561 cataaacgcc tcggtatcat gtatatcatc gtggcgattg tgatgttgct gcgtggtttt
    13621 gctgacgcca ttatgatgcg tagccagcag gctcttgcct cggcgggcga agcgggcttc
    13681 ctgccacctc accactacga tcagatcttc accgcgcacg gcgtgattat gatcttcttc
    13741 gtggcgatgc ctttcgttat cggtctgatg aacctggtgg ttccgctgca gatcggcgcg
    13801 cgtgacgttg cgttcccgtt cctcaacaac ttaagcttct ggtttactgt tgttggtgtg
    13861 attctggtta acgtttctct cggcgtgggc gaatttgcgc agaccggctg gctggcctat
    13921 ccaccgctat cgggaataga gtacagtccg ggagtcggtg tcgattactg gatatggagt
    13981 ctccagctat ccggtatagg tacgacgctt accggtatca acttcttcgt taccattctg
    14041 aagatgcgcg caccgggcat gaccatgttc aagatgccag tatttacctg ggcatcactg
    14101 tgcgcgaacg tactgattat tgcttccttc ccaattctga cggttaccgt cgcgttgttg
    14161 accctggatc gctatctggg cacccatttc tttaccaacg atatgggtgg caacatgatg
    14221 atgtacatca acctgatttg ggcctggggc cacccggaag tttacatcct gatcctgcct
    14281 gttttcggtg tgttctccga aattgcggca accttctcgc gtaaacgtct gtttggttat
    14341 acctcgctgg tatgggcaac cgtctgtatc accgtgctgt cgttcatcgt ttggctgcac
    14401 cacttcttta cgatgggtgc gggcgcgaac gtaaacgcct tctttggtat caccacaatg
    14461 attatcgcca tcccgaccgg ggtgaagatc ttcaactggc tgttcaccat gtatcagggc
    14521 cgcatcgtgt tccattctgc gatgctgtgg accatcggtt ttatcgtcac tttctcggtg
    14581 ggcgggatga ctggcgtgct gctggcagta ccgggcgcgg acttcgttct gcataacagc
    14641 ctgttcctga ttgcgcactt ccataacgtt atcatcggcg gcgtggtctt cggctgcttc
    14701 gcagggatga cctactggtg gcctaaagcg ttcggtttca aactgaatga aacctggggt
    14761 aaacgcgcgt tctggttctg gatcatcggc ttcttcgttg cctttatgcc gctgtatgcg
    14821 ctgggcttca tgggcatgac ccgtcgtttg agccagcaga ttgacccgca gttccacacc
    14881 atgctgatga ttgcagctag cggtgcagta ctgattgcgc tgggtattct ctgcctcgtt
    14941 attcagatgt acgtttctat tcgcgaccgc gaccagaacc gtgacctgac tggcgacccg
    15001 tggggtggcc gtacgctgga gtgggcaacc tcttccccgc ctccgttcta taactttgcc
    15061 gttgtgccgc acgttcacga gcgtgatgca ttctgggaaa tgaaagagaa aggcgaagcg
    15121 tataaaaagc ctgaccacta tgaagaaatt catatgccga aaaacagcgg tgcaggtatc
    15181 gtcattgcag ctttctccac catcttcggt ttcgccatga tctggcatat ctggtggctg
    15241 gcgattgttg gcttcgcagg catgatcatc acctggatcg tgaaaagctt cgacgaggac
    15301 gtggattact acgtgccggt ggcagaaatc gaaaaactgg aaaaccagca tttcgatgag
    15361 attactaagg cagggctgaa aaatggcaac tgatactttg acgcacgcga ctgcccacgc
    15421 gcacgaacac gggcaccacg atgcaggcgg aaccaaaatc ttcggatttt ggatctacct
    15481 gatgagcgac tgcattctgt tctcaatctt gtttgctacc tatgccgttc tggtgaacgg
    15541 caccgcaggc ggcccgacag gtaaggacat tttcgaactg ccgttcgttc tggttgaaac
    15601 tttcttgctg ttgttcagct ccatcaccta cggcatggcg gctatcgcca tgtacaaaaa
    15661 caacaaaagc caggttatct cctggctggc gttgacctgg ttgtttggtg ccggatttat
    15721 cgggatggaa atctacgaat tccatcacct gattgttaac ggcatgggtc cggatcgcag
    15781 cggcttcctg tcagcgttct ttgcgctggt cggcacgcac ggtctgcacg tcacttccgg
    15841 tcttatctgg atggcggtgc tgatggtaca aatcgctcgt cgcggcctga ccagcactaa
    15901 ccgtacccgc atcatgtgcc tgagcctgtt ctggcacttc ctggatgtgg tttggatctg
    15961 tgtgttcact gttgtttatc tgatgggggc gatgtaatga gtcattctac cgatcacagc
    16021 ggcgcgtccc atggcagcgt aaaaacctac atgacaggct ttatcctgtc gatcattctg
    16081 acggtgattc cgttctggat ggtgatgaca ggagctgcct ctccggccgt aattctggga
    16141 acaatcctgg caatggcagt ggtacaggtt ctggtgcatc tggtgtgctt cctgcacatg
    16201 aataccaaat cagatgaagg ctggaacatg acggcgtttg tcttcaccgt gctaatcatc
    16261 gctatcctgg ttgtaggctc catctggatt atgtggaacc tcaactacaa catgatgatg
    16321 cactaagagc ggcggttatg atgtttaagc aatacctgca agtaacgaaa ccaggcatca
    16381 tctttggcaa cctgatctcg gtgattgggg gattcctgct ggcctcaaag ggcagcattg
    16441 attatcccct gtttatctac acgctggttg gggtgtcact ggttgtggcg tcgggttgtg
    16501 tgtttaacaa ctacatcgac agggatatcg acagaaagat ggaaaggacg aagaatcggg
    16561 tgctggttaa aggcctgatc tctcctgctg tctcgctggt gtacgccacc ttgctaggct
    16621 ttgctggctt tatgctgctg tggtttggcg cgaatccgct ggcctgctgg ctgggggtga
    16681 tgggctttgt ggtttatgtc ggcgtttata gcctgtacat gaaacgccac tcggtctacg
    16741 gcacattgat tggttcgctc tccggcgctg cgccgccggt gattggctac tgtgcagtaa
    16801 ccggcgagtt cgatagcggc gcagcgatcc tgctggctat cttcagcctg tggcagatgc
    16861 ctcactccta tgccatcgcc attttccgct ttaaggatta tcaggcggca aacattccgg
    16921 tattgccagt ggtaaaaggc atttcggtgg cgaagaatca catcacgctg tatatcatcg
    16981 cctttgccgt tgccacgctg atgctctctc ttggcggtta cgctgggtat aaatatctgg
    17041 tggtcgccgc ggcggtgagc gtctggtggt taggtatggc cctgcgcggt tataaagttg
    17101 ctgatgacag gatctgggcg cgcaagctgt tcggcttctc tatcatcgcc atcacagccc
    17161 tctcggtgat gatgtccgtt gattttatgg tgccggactc gcatacgctg ctggctgctg
    17221 tttggtaaca caacctctct attaaaaagg tgctacggca ccttttttat tgtctgagat
    17281 cagagatacg gctaataacc gtatgtcttg acagagtgag cagatgaaca taaattaatc
    17341 atacggttga tagccgtatt atgtatgaga tcgactccct acgatttcat tcagttaatc
    17401 aaagaggtca taatgcctgg aaaaacagcc actctacccg atgtcgataa aacgctgaaa
    17461 aatgcgcggg tcgaactcaa aactagtccc gacgccaaaa acaaacttcg tgaagctgcg
    17521 caagccgttg gggtagattt aagcgctttt attttgagcg ctgctatgga acgcgccgaa
    17581 agtgtgcttg ataaccaacg tcgccgtgag ctttcgaatc aaagctggga actgatgaac
    17641 caactcatcg ctgaacctgc tcaaccgacg ctcgccctca aggcgttaat gaaaaggaaa
    17701 aacagcgatg gtcgacaagc atgaagagat tactctgccc atagtcctct cctgtaatta
    17761 tcagtctgat attacttatc ctgggcaaaa acagtttgat tgcggtaact ctgttatcga
    17821 taaatttgta cgcgcatcgc taaagaaaag tgtgcgtaat agcgactgtg cggctaaagc
    17881 acttattgac agacaaagtg gtgaactgat cggcatctgt acttttacgg catattcgct
    17941 ggaaaaacaa cgcgtttctg gcgtccttca gggttcacaa ccttcagaaa ttggtgttgt
    18001 cagattagtc atgttggggg tagcacggaa gtatcaaaag cggggctttg gtcaggacct
    18061 actatgtgat ttttttgaac atgtaaaaat aattcaccag gcattaccaa ttaaaggggt
    18121 ttatcttgat gctgaccctg ccgccattaa tttttatgct cgtctcggct ttgttcagct
    18181 ttcagcgaca ccaaatgctt ttggtgctgt acctatgttt ttggcgattc agcatattct
    18241 cgcggcttag attattatcg ccacccgcca gggcaagttt tgccttagcc gggggctgag
    18301 cagcgttacc acttttgttc aattgccccc gctttcagca actccacatc cttgccgtta
    18361 atggttgccg ttgcggcaac gattgtggtc cttacagttg cacaagattt tcgctcaaat
    18421 tccggtttat aaagaataag agacgaatta ctggtaaatg aaccaaatag gtaatcagta
    18481 aagctaatat tcagccggtt taaaccgaaa ggcgcgaagc caaccgtttg gttaatctcc
    18541 ttaccggatg ggcgacgaat gctgaatgtc acgcgaagat tggccacttc gcctaattta
    18601 ttggtggtgg cgaagaggct atccatgcag gaaaaacctt tatctaaaaa attatctcgc
    18661 atcccagaac gggtaaccgt aatcacgggt aacgtatcgt caaccgtttt tctgtcaatc
    18721 atgtcttttc gacaagtagc cagaaagtct tccgggccac cataaatgtg gacaccttta
    18781 tcaagatatt ccttcgcttt cttacaatct acctcaacat actgaccatt ggcgtacata
    18841 atgccc
//
LOCUS       DAZPLM010000068        18501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPLM010000068 DAZPLM010000000
VERSION     DAZPLM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.068"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHVC7_002015"
     CDS             complement(<1..331)
                     /locus_tag="ACHVC7_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR2116069.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
     gene            483..812
                     /locus_tag="ACHVC7_002016"
     CDS             483..812
                     /locus_tag="ACHVC7_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR2116070.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1060..1305
                     /gene="nrdH"
                     /locus_tag="ACHVC7_002017"
     CDS             1060..1305
                     /gene="nrdH"
                     /locus_tag="ACHVC7_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR2116071.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1302..1712
                     /gene="nrdI"
                     /locus_tag="ACHVC7_002018"
                     /pseudo
     CDS             1302..1712
                     /gene="nrdI"
                     /locus_tag="ACHVC7_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            1685..3829
                     /gene="nrdE"
                     /locus_tag="ACHVC7_002019"
     CDS             1685..3829
                     /gene="nrdE"
                     /locus_tag="ACHVC7_002019"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR2116072.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3839..4798
                     /gene="nrdF"
                     /locus_tag="ACHVC7_002020"
     CDS             3839..4798
                     /gene="nrdF"
                     /locus_tag="ACHVC7_002020"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR2116073.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5154..6356
                     /gene="proV"
                     /locus_tag="ACHVC7_002021"
     CDS             5154..6356
                     /gene="proV"
                     /locus_tag="ACHVC7_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR2116074.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6349..7413
                     /gene="proW"
                     /locus_tag="ACHVC7_002022"
     CDS             6349..7413
                     /gene="proW"
                     /locus_tag="ACHVC7_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR2116075.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7471..8463
                     /gene="proX"
                     /locus_tag="ACHVC7_002023"
     CDS             7471..8463
                     /gene="proX"
                     /locus_tag="ACHVC7_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR2116076.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8754..9938
                     /locus_tag="ACHVC7_002024"
     CDS             8754..9938
                     /locus_tag="ACHVC7_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2116077.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10062..10799
                     /gene="ygaZ"
                     /locus_tag="ACHVC7_002025"
     CDS             10062..10799
                     /gene="ygaZ"
                     /locus_tag="ACHVC7_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR2116078.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10789..11124
                     /gene="ygaH"
                     /locus_tag="ACHVC7_002026"
     CDS             10789..11124
                     /gene="ygaH"
                     /locus_tag="ACHVC7_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR2116079.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11215..11745
                     /gene="emrR"
                     /locus_tag="ACHVC7_002027"
     CDS             11215..11745
                     /gene="emrR"
                     /locus_tag="ACHVC7_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR2116080.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            11872..13044
                     /gene="emrA"
                     /locus_tag="ACHVC7_002028"
     CDS             11872..13044
                     /gene="emrA"
                     /locus_tag="ACHVC7_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR2116081.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13061..14599
                     /gene="emrB"
                     /locus_tag="ACHVC7_002029"
     CDS             13061..14599
                     /gene="emrB"
                     /locus_tag="ACHVC7_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR2116082.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14663..15178)
                     /gene="luxS"
                     /locus_tag="ACHVC7_002030"
     CDS             complement(14663..15178)
                     /gene="luxS"
                     /locus_tag="ACHVC7_002030"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR2116083.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15328..16884)
                     /gene="gshA"
                     /locus_tag="ACHVC7_002031"
     CDS             complement(15328..16884)
                     /gene="gshA"
                     /locus_tag="ACHVC7_002031"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR2116084.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(16957..17385)
                     /locus_tag="ACHVC7_002032"
     CDS             complement(16957..17385)
                     /locus_tag="ACHVC7_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR2116085.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17382..17948)
                     /gene="yqaB"
                     /locus_tag="ACHVC7_002033"
     CDS             complement(17382..17948)
                     /gene="yqaB"
                     /locus_tag="ACHVC7_002033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR2116086.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18229..18305)
                     /locus_tag="ACHVC7_002034"
     tRNA            complement(18229..18305)
                     /locus_tag="ACHVC7_002034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18269..18271),aa:Arg,seq:acg)
BASE COUNT         4386 a         4816 c         4699 g         4600 t
ORIGIN      
        1 caaataaacc attcaaataa cgttcaagcg caacacgcga actgaagcca tgcgggatac
       61 cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgttgataa tgatcgcaag
      121 ttttaatatc ggaagcgggt tcagccagtg tcgaactacg ttcttttaat gttggatgaa
      181 ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac
      241 ggcggtaacc ataagctttc tgggttacca catcgacagt aaaaccgact ttttcaagta
      301 cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttattat caaccgcggg
      361 cctaccttac ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga
      421 acctcagtta agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc
      481 atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg
      541 atgtcaatca attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca
      601 aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt gctgaaagaa accagggcgc
      661 gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga tgccgttggc tgcgcggatt
      721 cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta
      781 tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt
      841 taaccccgaa gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa aatccccctc
      901 ccccgtcacg ctcatatcta gggtaatttc gatcactatt tgctatatat tgtgtggttg
      961 aatctttttt caactacatc tagtatctct gtatcaacag agagacaacc cgacgcgtat
     1021 catcgcgccg tatcttcatt ttaaacggaa atacgaatca tgcgcataac tatttacact
     1081 cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg gggctttgat
     1141 tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg aagcgttgcg tgctcagggc
     1201 tttcgtcagt tgccggtagt gattgctggc gatcttagct ggtctggttt ccgtccggac
     1261 atgattaacc gtctgcatcc agcgccacac gcggccagtg catgagccag ctcgtctact
     1321 tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc
     1381 gcatcccgct caatgagcgg gaacggattc aggtagacga gccttacatc ctgatcgtgc
     1441 cctcttacgg cggcggcggt acggctggcg cggtgccacg ataggtaatt cgctttttaa
     1501 acgacgagca caaccgggcg ttgcttcgcg gcgttattgc ttctggtaat cgcaactttg
     1561 gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt
     1621 accgttttga actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg
     1681 aattttggca acgacaaccg cagaatgcct gacgcaggaa acgatggatt accacgcgct
     1741 gaatgcgatg cttaacctct acgatagcgc aggtcgcatt cagttcgata aagaccgcca
     1801 ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca
     1861 gcagcagcgc ctgaactggc tggtcaacga aggttactat gatgaaagcg ttcttaatcg
     1921 ctactctcgc gactttgtca ttacgctgtt tgcccacgca cacaccagcg gttttcgttt
     1981 ccagacattc ctcggggcat ggaagtttta caccagctat acgttgaaga cattcgacgg
     2041 taaacgttat ctggaagatt ttgccgatcg agtcacgatg gtggcgctga cgctggcaca
     2101 aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc
     2161 agccacgccg acattcctca actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt
     2221 tctgctgcgt attgaagaca atatggagtc gattggccgg gcggtaaatt ccgcactgca
     2281 actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg aatctgcgag aagcgggcgc
     2341 gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga aaatgctgga
     2401 agacgcattt tcctatgcca accaactcgg cgcgcgtcag ggggcaggtg cagtctattt
     2461 acatgctcat catcccgata ttctgcgttt tctcgataca aaacgggaaa atgccgacga
     2521 aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc ccggatatca ctttccatct
     2581 ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc gagtttatgg
     2641 caagccgttt gccgatatcg ccatcagcga acactatgac gaactggttg ccgatgaacg
     2701 catccgcaaa aaatacctca acgcccgtga tttcttccag cggctggcag aaatccagtt
     2761 tgagtccggc tatccctaca tcatgtatga agacacggta aaccgtgcta accctgtcgc
     2821 cgggcgcata aatatgagta atctctgctc agagattttg caggtcaact gtgcctcaga
     2881 gtatgacgag aatctcgact atgcccgcac aggccatgat atctcctgca atttaggttc
     2941 gttgaatatt gcgcacacca tggattcccc cgattttgcc cgcacggtag agactgccgt
     3001 gcgcggttta acggcggtgt cagatatgag tcatatccgc agcgtgccgt ccatcgaagc
     3061 cggaaatgcc gcctcacacg ccatcggact ggggcagatg aatttacacg gctatctggc
     3121 gcgagaaggc atcgcttatg gttcgccgga agcactggat ttcaccaatc tctatttcta
     3181 tgccatcacc tggcacgcac tgcgtacctc gatgttgctg gcgcgcgaac gcggtgaaac
     3241 cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa tattttagcc aatatctgca
     3301 aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg tttgcccgca gcggtattac
     3361 gttacctacc cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct acggcattta
     3421 caaccagaat cttcaggcgg tgccgccaac cggttctatc tcttatatca accatgctac
     3481 gtcgagtatt catccgattg tggcgaaagt agagatacgc aaagagggca aaacaggacg
     3541 cgtttactac cctgccccgt ttatgactaa cgaaaatctg gcgctgtatc aggatgctta
     3601 cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca
     3661 gggtttgtcg ctgacgctgt ttttccccga taccgccacc actcgcgata tcaacaaagc
     3721 gcagatttac gcctggcgca aaggtatcaa aacgctctat tacattcgcc tgcgtcagat
     3781 ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag gaatatctat
     3841 gaaactctca cgtatcagcg ccatcaactg gaacaagata tctgacgata aagatctgga
     3901 ggtgtggaat cgcctgacca gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga
     3961 tattcctgcc tggcagacat taactgtcgt agaacaacaa ctgacgatgc gcgtttttac
     4021 tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga
     4081 tgcactcacg cctcatgaag aagcggtatt atcgaatatc agctttatgg aagcggttca
     4141 tgcccgctct tacagttcga ttttctcgac gctgtgccag accaaagatg tcgatgccgc
     4201 ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca
     4261 ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt
     4321 gttctattcc ggtttctggc tgccgatgta tttttccagc cgcggaaagc tgaccaatac
     4381 cgcagacctg atccgtctga ttatccgtga tgaagcagtc cacggttact acataggcta
     4441 taaatatcag aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt tgaagagttt
     4501 cgccttcgat ttgttgctgg aactctacga caacgagctg caatacaccg atgagctgta
     4561 cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc tgttacaacg ccaataaggc
     4621 tttgatgaat ctgggctacg aacctttatt tcccgcagaa atggcggaag tgaatccggc
     4681 aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac gatttctttt ccggttcagg
     4741 ctcctcttat gtgatgggga aagcggttga aacagaagat gaagactgga atttctgagg
     4801 gtgttatttt caaaaatatc actacccgcg acagggaaat aattcccgcc aaatagcttt
     4861 ttatcacgca aatattttgt ggtgatctac actgatactc tgttgcatta ttcgcctgaa
     4921 accacaatat tcaggcgttt tttcgctatc tttgacaaaa aatatcaact tttctcgatt
     4981 tgctctcagc ccttatatca cgggaaattc cggcgatttg ctcgcatcaa tattcatgcc
     5041 acatttgcca tcaggggttg cctcagattc tcagtatgtt agggtagaaa aaagtaacta
     5101 tttccattgg gtaatatatc gacataaaca aataacagga atctttctat tgcatggcaa
     5161 ttaaattaga aattaaaaac ctttataaaa tatttggtga gcatccacag cgagcattca
     5221 aatatatcga acaaggactt tcaaaagaac aaattctgga aaaaactggg ctatcgcttg
     5281 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
     5341 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
     5401 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccaaa ctccgtgagg
     5461 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
     5521 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
     5581 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
     5641 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
     5701 cggatatttt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     5761 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     5821 acgatctcga tgaagccatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     5881 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     5941 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     6001 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     6061 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     6121 cagtctccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     6181 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     6241 tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca
     6301 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     6361 aataatccgt gggataccac gccagcggcg gacagtgccg cgcaatccgc agacgcctgg
     6421 ggtacaccga cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     6481 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     6541 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     6601 ggcgtgcgcg ttccggttga ttatatcctc aacagtttcc agcaattgct gctgggtatg
     6661 ccagcgccag tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     6721 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     6781 gcaatggtga ctctggcgct ggtgttaacc gccctgctgt tctgtatcgt catcggtttg
     6841 ccgttgggga tatggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     6901 gatgccatgc agaccacgcc agcgtttgtt tatctggtac caatcgtcat gctgtttggt
     6961 atcggtaacg tgccgggcgt agtggtgacg atcatctttg ctctgccgcc gattatccgt
     7021 ctgacgattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     7081 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgaccatt
     7141 atggcgggcg ttaaccagac gctgatgctg gctctttcta tggtggtcat cgcctcgatg
     7201 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggc
     7261 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga ccgcctgacg
     7321 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     7381 gtcggtctgc tgacccgccc attcattaag taactctgca cttgcccggt aacgccgggc
     7441 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     7501 tttgccacgc ttatctctac acaaactttt gcagccgatc tgccgggcaa aggcattact
     7561 gttaatccag ttcagagcac catcactgaa gaaaccttcc agacgctgct ggtcagtcgt
     7621 gcactggaga aattaggtta taccgtcaat aaacccagcg aagtggatta caacgttggc
     7681 tacacctcac ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccgctgcat
     7741 gacaacatgt acgaagctgc cggtggcgat aagaaatttt attgtgaagg ggtattcgtt
     7801 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     7861 aacatcgcac aactgaaaga tccgaagatc gccaaactgt tcgataccaa cggcgacggt
     7921 aaagcggatt taaccggctg taacccaggc tggggctgcg aaggtgcgat caaccaccag
     7981 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     8041 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     8101 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     8161 ttctccgcac tgccgggcga taaaaacgcc gataccaaac tgccgaatgg tgcgaattat
     8221 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctgggccga gaaaaacccg
     8281 gcagcagcga aactttttgc cattatgcag ttgccagtgg cagatattaa cgcccagaac
     8341 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccatgt tgatggttgg
     8401 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgcagaag
     8461 taatttttat tcgggcggat aagtcgtttt tgcctcatcc gccattctgt gcacaatgcc
     8521 tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     8581 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     8641 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcgcc cccattcatc
     8701 aacatccgct attattgatt tccagcttaa tcatcacctg atgaacaaaa ataatgacta
     8761 aacctaatca tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc accggtcagg
     8821 cggtagccag taactattac gcccagccat tgctcgacac catcgcgcgt aacttttccc
     8881 tttccgccag ttcggcaggc tttattgtta ccgccgcgca gttgggctat gcagcaggtc
     8941 tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga
     9001 ccttactggc ggcaggcggc atgttaatca ctgccagcag tcagtcgctg gcgatgatga
     9061 tcctcggtac ggcattaacc ggtttattct cagtcgtggc acaaattctg gttccgctgg
     9121 cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt atgagcggtc
     9181 tgctgttggg gatcttgctg gcacgaaccg ttgccgggtt actggcaaac ctcggcggct
     9241 ggcgcaccgt cttttgggtt gcctcggtgt taatggcact gatggcgcta gcattatggc
     9301 gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg ttaggttccg
     9361 ttttcagcat gtttatcagc gataagatcc tccgcacccg cgcattgctg ggctgcctga
     9421 cctttgccaa cttcagcatt ctctggacct caatggcctt tttgcttgcc gctccacctt
     9481 ttaactacag cgatggtgtc attggtctgt ttggacttgc gggagctgcc ggagcgttgg
     9541 gcgctcgtcc ggcgggcggt tttgccgata agggtaaatc gcaccacacc acaactttcg
     9601 gtctgctgct gctattactt tcatggctgg cgatctggtt tggtcacact tccgtactgg
     9661 cgctgattat cggcatcctg gtgctggatc tcaccgtaca gggcgtgcat atcactaacc
     9721 agacggtaat ttatcgaata caccctgatg cgcgtaatcg cctgaccgca ggttacatga
     9781 ccagctactt tattggcggt gccgccggtt cgctaatttc agcctcagcc tggcaacatg
     9841 gcggttgggc tggcgtttgt ctggctggcg cgacgattgc cctagttaac ttactggtct
     9901 ggtggcgagg ttttcatcgt caggaagccg caaattaagc aatcagcaac cgttttcgtg
     9961 gctttacact tataagggtg ttaagaagcc catcagtctg ataaggttaa gatattcatt
    10021 cagtctattt ataatattaa caatcgttaa gcgtacactc tatggaaagc cctactccac
    10081 agcctgctcc tggttcggcg accttcatgg aaggatgcaa agacagttta ccgattgtta
    10141 ttagttatat tccggtggcc tttgcgttcg gtctgaatgc gacccgtctg ggattctctc
    10201 ctctcgaaag cgtttttttc tcctgcatca tttatgcagg cgcgagccag ttcgtcatta
    10261 ccgcgatgct ggcagccggg agtagtttgt gggttgctgc actgaccgtc atggcaatgg
    10321 atgttcgcca tgtgttgtat ggcccgtcac tgcgtagccg tattattcag cgtctgcaaa
    10381 aatcgaaaac cgccctgtgg gcgtttggcc tgacggatga ggtttttgcc gccgcaacag
    10441 caaaactggt acgcaataat cgccgctgga gcgagaactg gatgatcggc attgccttca
    10501 gttcatggtc atcgtgggta tttggtacgg taataggggc attctccggc agcggcttgc
    10561 tgcaaggtta tcccgccgtt gaagcagcat taggttttat gcttccggca ctctttatga
    10621 gtttcctgct cgcctctttc cagcgcaaac aatctctttg cgttaccgca gcgttagttg
    10681 gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc cattctggca ggcattgtct
    10741 gtggctgcct cactgcgtta atccaggcat tctggcaagg agcgcccgat gagctatgag
    10801 gttctgctgc ttgggttact ggttggcgcg gcgaattatt gcttccgcta tttgccgctg
    10861 cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg cggtaggtat tttgctcgac
    10921 accattggca tcgcctcgat atgcgctctg ctggttgtct ctaccgcacc agaagtgatg
    10981 cacgatacac gccgtttcgt gcccacgctg gtcggcttcg cggtactggg tgccagtttc
    11041 tataaaacac gcagcattat catcccaaca ctgcttagtg cgctggccta tgggctcgcc
    11101 tggaaagtga tggcgattat ataacccaca agaatcattt ttctaaaaca atacatttac
    11161 tttatttgtc actgtcgtta ctatatcggc tgaaattaat gaggtcatac ccaaatggat
    11221 agttcgttta cgcccattga acaaatgcta aaatttcgcg ccagccgcca cgaagatttt
    11281 ccttatcagg agatccttct gactcgtctt tgcatgcaca tgcaaagcaa gctgctggag
    11341 aaccgcaata aaatgctgaa ggctcagggg attaacgaga cgttgtttat ggcgttgatt
    11401 acgctggagt ctcaggaaaa ccacagtatt cagccttctg aattaagttg tgctcttgga
    11461 tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa
    11521 cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac
    11581 gagtttttgc gcgaggtttt accaccgcag cataactgcc tgcatcaact ctggtccgcg
    11641 ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc gcaaattgct ctcccgtctc
    11701 gaccagatgg aacaagacgg tgtggttctc gaagcgatga gctaacgcgt catctcgctc
    11761 aaaaatccag atttataaaa gaaaaaatga ccggccagca tcgcaacatg ctggcctttt
    11821 tggcaagcag gtcggctcag ccgatgagtt aagaagatcg tggagaacaa tatgagcgca
    11881 aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga gcggcaaacg taagcgtctg
    11941 ctcctccttc tcaccttgct ctttataatt attgccgtag cgatagggat ttattgtttt
    12001 ttggtactgc gtcatttcga agaaaccgat gacgcatacg tggcagggaa tcagattcaa
    12061 attatgtctc aggtgtctgg cagcgtgacg aaagtctggg ccgataacac cgattttgta
    12121 aaagaaggcg acgtgctggt cactctcgac ccgacagatg ctcgccaggc gtttgaaaaa
    12181 gccaaaactg cactggcttc cagcgttcgc caaacccacc agctgatgat taacagcaaa
    12241 cagttgcagg cgaatattga ggtgcagaaa atcgccctcg caaaagcaca aagcgactac
    12301 aaccgccgtg tgccgctggg caatgccaac ctgattggtc gcgaagagct gcaacacgcc
    12361 cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg ctattcaaca atacaatgcc
    12421 aatcaggcga tgattctggg gactaaactg gaagatcagc ctgccgtgca acaggctgcc
    12481 accgaagtac gtaacgcctg gctggcgctg gagcgtaccc gtattgtcag tccgatgacc
    12541 ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa ttagcccaac gacgccgctg
    12601 atggcggtcg ttccagccac caatatgtgg gtggatgcca actttaaaga gacgcagatt
    12661 gccaatatgc gtatcggtca gccggtcact atcaccacgg atatttacgg cgatgatgtg
    12721 aaatacaccg gtaaagtggt tggtctggat atgggcacag gtagcgcatt ctcactgctt
    12781 ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc agcgtctacc tgtgcgtatc
    12841 gaactggacc agaaacagct ggagcaatac ccgctgcgca ttggtttgtc cacactggtg
    12901 agcgtcaata ccactaaccg tgacggtcag gtactggcaa ataaagtacg ttcaacaccg
    12961 gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg tcaataaact gatcgacgat
    13021 atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg atgcaacagc aaaaaccgct
    13081 ggaaggcgcg caactggtca ttatgacgat tgcgctgtca ctggcgacat tcatgcaggt
    13141 gctggactcc accattgcta acgtggcgat ccccactatc gccgggaatc tgggctcatc
    13201 gctcagccag ggaacgtggg taatcacttc tttcggggtg gcgaatgcca tctcgatccc
    13261 gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa ctgttccttt ggtccaccat
    13321 cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc agcctgaata tgctgatctt
    13381 cttccgcgtg attcagggga ttgtcgccgg gccgttgatc ccgctttcgc aaagtctatt
    13441 gctgaataac tacccgccag ccaaacgctc gatcgcgctg gcgttgtggt cgatgacggt
    13501 gattgtcgcg ccaatttgcg gcccgatcct cggcggttat atcagcgata attaccactg
    13561 gggctggata ttcttcatca acgtgccgat tggcgtggcg gtggtgttga tgacactgca
    13621 aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg attgatgccg tggggctggc
    13681 actgctggtt attggtatcg gcagcctgca gattatgctc gaccgcggta aagagctgga
    13741 ctggttttca tcacaggaaa ttatcatcct taccgtggtg gcggtggtgg ctatctgctt
    13801 cctgattgtc tgggagctga ccgacgataa cccgatagtc gatctgtcgt tgtttaagtc
    13861 gcgcaacttc accatcggct gcttgtgtat cagcctcgcg tatatgctct acttcggcgc
    13921 tattgttctg ctgccgcagt tgttgcagga ggtctacggt tacacggcga cctgggcagg
    13981 tttggcctct gcgccggtag ggattattcc ggtgatcctg tcgccgatta tcggccgctt
    14041 cgcgcataaa ctggatatgc ggcggctggt aaccttcagc tttattatgt atgccgtctg
    14101 cttctactgg cgtgcctata cctttgaacc aggtatggat tttggcgcgt cggcctggcc
    14161 gcagtttatc caggggtttg cggtggcctg cttctttatg ccgctgacca ccattacgct
    14221 gtctggtttg ccaccggaac gactggcggc ggcatcgagc ctctctaact ttacgcgaac
    14281 gctggcgggg tctatcggca cgtcgataac cacgaccatg tggaccaacc gcgagtcgat
    14341 gcaccatgcg cagttgactg agtcggtaaa cccgttcaac ccgaatgccc aggcgatgta
    14401 cagtcaactg gaagggcttg ggatgacgca acagcaggca tcaggctgga ttgcccagca
    14461 gatcaccaat caggggctga ttatttccgc caatgagatc ttctggatgt cagccgggat
    14521 attcctcgta ctgctggggc tggtgtggtt tgctaaaccg ccatttggcg caggtggcgg
    14581 tggcggcggt gcgcactaag tacaactaag ccagttcatt tgaactggct tttttcaatt
    14641 aattgtgaag atagtttact gactagatgt gcagttcctg caacttctct ttcggcagtg
    14701 ccagttcttc gttgctgttg atgcgtacgt cacgttccag aatgctacgc gcaatatcct
    14761 gcgcttcctg caacgagtgc atctggtaag tgccacactg gtagacgttc agttccggga
    14821 tctgattctg atcctgcact ttcagcacgt cttccattgc cgctttccag gcatcagcaa
    14881 cacgctgctc atctggcgta ccaatcagac tcatataaaa accggtgcgg cagcccattg
    14941 gcgagatatc gataatctct acaccattac cgttaagatg gttacgcata aaaccagcaa
    15001 acaggtgctc cagggtatgg atccctcttt ctggcatcac ttctttgttc ggcacgcaga
    15061 agcgcagatc gaacacggtg attgcgtcgc catgcggggt gttcattgtt ttcgccaccc
    15121 gaactgcagg cgcttccatc cgggtatgat cgactgtgaa gctatctaac aacggcattt
    15181 agccacctcc ggtaattttt ttaaaaattt tctgaactct ttcttcccag gcgagtctga
    15241 gtatatgaaa gacgcgcatt tgttatcatc atccctgaat tcagagatga aattttggcc
    15301 actcacgagt ggcctttttc ttttctgtca ggtgtgtttt tccagccaca ccgcaaacgg
    15361 ttcggtatca gcggtttcca tttcctgctg acggcgttca gatgcctcgc gctcggctac
    15421 aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc agcagattac ggtaggcttc
    15481 tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc atagacctta agatacgggc
    15541 agagaaagtc agatcgggat tatcgaagca ggcaaccagt tcatcacaca ctttctgata
    15601 cgcgtcgccg ccgttaatgc tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag
    15661 atctttaccc acctgcagta acgggaactg cgcggtttcg cagccgatac ccagcgtcag
    15721 gcccggtttg cgaccttcaa ggatcacccg gttccagtta acgcgcgtac aggcaagttc
    15781 gctactgctc atttccggtg catcagccag cgcacaccag accataaaca ggtcgaggaa
    15841 tcgcacctgc tgttcatcta caccaatcgg cgagaacggg ttgatgtcca gcgaacgcac
    15901 ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa ggtgactcgc cgctgcgggt
    15961 aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca atctgcagca cgttgctatt
    16021 gatttgcagc ctcttaccgt ctttctcaat accaatcttc gcgtactctt ccgatggcgt
    16081 tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga tcgttgaagg taataccaag
    16141 attgctttgc gatttattgg tatagccgag atcgctcaaa cgaagagagg tcgcatacgg
    16201 caggtaatac ataccgcact cggttttctc aaacggcagc gacgttggtt ttccttgcag
    16261 gaaagaagaa caaatcgccg gagatgcacc aaacagataa ggaatgaccc aaccgaaacg
    16321 atagtaattg cggataacgc ggaaatagcc cgcagaaatt ttctctttgg catcagcgcc
    16381 cgagatatca ccgcacttcg cttgccagaa tgccattggc aaagagaaat tgtagtgcac
    16441 gccggaaatg gtttgcatca gcgcgccgta gcgatttttc agcccttcac gatacagcgt
    16501 tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt tcgatgtcct gaccttctgc
    16561 gatgtagcat ggcatactta acggccacat ccgctcatcg cccatattgc gcgccgtata
    16621 acgatgcaga tcgcgcataa aggtcagcat atgttcaata tcaccatcca ctggtgtaat
    16681 gaattccagc aatgcttccg caaaatcggt agtaatccat ttgtgcgtca gtgcggaacc
    16741 taatgcttca ggatgacctg ttgttgccag tgtgccatca gcattaacac gcaaagtttc
    16801 gcgctccagc ccacgctgta tcccctttaa cgcctgagga tgtttttcca gccaggccag
    16861 cgcctgtgat acgtccggga tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc
    16921 ataactgtaa tggtgaccat atgtgcaggc ctacaattag tgccaccaca tcatgccctg
    16981 aacggtcgct gctgcaactg caacatagcg taacgcttta ccaaggcaca aaaaaaagat
    17041 taccggtccc cacgagatgc gcatccatcc cgctaacaga cacagtaaat cgccaaccac
    17101 gggcatccag cttaataata gcgtgactgc accatagcgt ttcagccagc cgatagcttt
    17161 ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc ccaaggataa cgttagttaa
    17221 ccctccaagg ctattaccca ttgttgctgt taaaactaaa acccagggat gactgatccc
    17281 ggaaagcaac attgccacca gcacgacttc ggagttgccg ggtaatagtg tagcgctgag
    17341 aaaactactg gcaaacaacg agaaaagcga taacgcttca ctcacagcaa gcgaacgtcc
    17401 acagcgtcca tacctgcggc acgggccgcc tgaataccga aatcggcatc ttcaaagacc
    17461 acacactgcg tcggttgcac gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg
    17521 ggtttatggt gtttgacgtg atcggcagcg acgacggcgt caaaataatg gcgtaatccc
    17581 aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc ccgttcctac agccattggg
    17641 cgacgaccat gccaactttt caccacatca acaagaggaa gcggttcgac gctatccagc
    17701 agcatacttc ttactgcttc tgttttttca cgcgctaacg catgcgggtc gagatcggcc
    17761 tgattcagct caataattgc ctgagcaata cgccaggtgg gcgatccatt aagcgcaatc
    17821 atcgcctgaa tatcgtactg aagaccgtag tgccctaata cttcgcgcca cgctttacgg
    17881 tgcgtaggct ccgtatccag gattgtgcca tccatatcaa aaattaaacc tgcataacgc
    17941 tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta attgcttgat
    18001 tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg gataaaggag ttatttagaa
    18061 atgagatatt tttgaaggaa attttttgga gaagatggtg catccgggag gatgactcac
    18121 tgacgttcgc ccttcgggcc gttgctggcg caacgttatc cttcctggtg cctgcggtta
    18181 ataccgctat ctcttcgata ccttcattgc tgaaggttac ggagaatatg gtgcatccgg
    18241 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
    18301 gatgcatcgg gaaaacttat tctactgctg atattgatac cgctactaaa gccatatcaa
    18361 ttaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc gggccgttgc
    18421 taaagcaacg ttatcatccc tggtgcctgc ggttaattcc gctacctctt cgataccttc
    18481 attgctgaag atttcggaga a
//
LOCUS       DAZPLM010000069        18372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPLM010000069 DAZPLM010000000
VERSION     DAZPLM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.069"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            48..680
                     /locus_tag="ACHVC7_002035"
                     /pseudo
     CDS             48..680
                     /locus_tag="ACHVC7_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            783..1285
                     /locus_tag="ACHVC7_002036"
                     /pseudo
     CDS             783..1285
                     /locus_tag="ACHVC7_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1352..2809)
                     /gene="nuoN"
                     /locus_tag="ACHVC7_002037"
     CDS             complement(1352..2809)
                     /gene="nuoN"
                     /locus_tag="ACHVC7_002037"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR2116087.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2816..4345)
                     /gene="nuoM"
                     /locus_tag="ACHVC7_002038"
     CDS             complement(2816..4345)
                     /gene="nuoM"
                     /locus_tag="ACHVC7_002038"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR2116088.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4509..6350)
                     /gene="nuoL"
                     /locus_tag="ACHVC7_002039"
     CDS             complement(4509..6350)
                     /gene="nuoL"
                     /locus_tag="ACHVC7_002039"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR2116089.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6347..6649)
                     /gene="nuoK"
                     /locus_tag="ACHVC7_002040"
     CDS             complement(6347..6649)
                     /gene="nuoK"
                     /locus_tag="ACHVC7_002040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR2116090.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6646..7200)
                     /gene="nuoJ"
                     /locus_tag="ACHVC7_002041"
     CDS             complement(6646..7200)
                     /gene="nuoJ"
                     /locus_tag="ACHVC7_002041"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR2116091.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7212..7754)
                     /gene="nuoI"
                     /locus_tag="ACHVC7_002042"
     CDS             complement(7212..7754)
                     /gene="nuoI"
                     /locus_tag="ACHVC7_002042"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR2116092.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7769..8746)
                     /gene="nuoH"
                     /locus_tag="ACHVC7_002043"
     CDS             complement(7769..8746)
                     /gene="nuoH"
                     /locus_tag="ACHVC7_002043"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR2116093.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8743..11469)
                     /gene="nuoG"
                     /locus_tag="ACHVC7_002044"
     CDS             complement(8743..11469)
                     /gene="nuoG"
                     /locus_tag="ACHVC7_002044"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR2116094.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11522..12859)
                     /gene="nuoF"
                     /locus_tag="ACHVC7_002045"
     CDS             complement(11522..12859)
                     /gene="nuoF"
                     /locus_tag="ACHVC7_002045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR2116095.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12856..13356)
                     /gene="nuoE"
                     /locus_tag="ACHVC7_002046"
     CDS             complement(12856..13356)
                     /gene="nuoE"
                     /locus_tag="ACHVC7_002046"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR2116096.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13359..15161)
                     /gene="nuoC"
                     /locus_tag="ACHVC7_002047"
     CDS             complement(13359..15161)
                     /gene="nuoC"
                     /locus_tag="ACHVC7_002047"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR2116097.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15255..15917)
                     /gene="nuoB"
                     /locus_tag="ACHVC7_002048"
     CDS             complement(15255..15917)
                     /gene="nuoB"
                     /locus_tag="ACHVC7_002048"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR2116098.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15933..16376)
                     /gene="nuoA"
                     /locus_tag="ACHVC7_002049"
     CDS             complement(15933..16376)
                     /gene="nuoA"
                     /locus_tag="ACHVC7_002049"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR2116099.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17005..17943)
                     /gene="lrhA"
                     /locus_tag="ACHVC7_002050"
     CDS             complement(17005..17943)
                     /gene="lrhA"
                     /locus_tag="ACHVC7_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR2116100.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4588 a         5098 c         4723 g         3963 t
ORIGIN      
        1 actcattata aataatgata aaataacgtc ttttattgat aatttatata tggaaaaaaa
       61 ttccttttgt tatattgttt ctgatttcac tccctctgat tatttttacc aaccctgggt
      121 cactgcatta tcgccaacgc cccgacacgt cagtcctgag caattagaaa aaacagttcg
      181 ttatcttaca caaactgtgc atccacgtaa tgccgacaat attgataatc tgaataggtc
      241 cgtagagtat ataaaagagt tctttgtcag tagcggtgcc agagttaccg cgcagagcgt
      301 ccccgattgg ctattacgac tctgcgcctg gtagccagga ttacccttac ccgcaatgtc
      361 ctggctttat cccgatcggg gggactttat tgcctggtcg gcagaataca ggatatcaac
      421 gccgttcgtc aggtaaaaac ggcattgtta tcatcccggg atttatctgt ttattctatg
      481 aatgccccat ggtttattcc cggtattgat ttctctgacc acctgaatta ttggcaacac
      541 gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg
      601 cccggtgata cagcagacag attgaattat cagaaaatgg ctcagatggt gttataacct
      661 tgttacacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
      721 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
      781 aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac caactgcttg
      841 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
      901 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc cctgcttttg
      961 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg
     1021 gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca atcgaccgac
     1081 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     1141 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcttcga
     1201 tcatgctgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac aagggaatca
     1261 tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc gccgaacatg
     1321 tcggcggctt tctgacttac aaactgatag cttacatcag cggcattgct aaccgcacaa
     1381 tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca gagatcagca
     1441 ccacgatacc gcctgcgctg tactgccagt ttgacggtgc atcgcgaccc ggttgttccg
     1501 gggcgtgcag gtacaggctc accgccacgc gcaggtagta gtaaaggccg cttgccgaac
     1561 cgacaaccac ggcgcccacc agccaccaca agtgtgcctg gacaccgacc gccagcacgt
     1621 agaacttacc aataaagccc agcgtcatcg gaataccggc cagagacagc atcatcaccg
     1681 tcatcaccgc cgcgaggatc gggcgatgcc agaacagtcc gcggtaagag tacagggaat
     1741 cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac gcgccgaggc
     1801 tgctaaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc tcgccagttt
     1861 gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag taaccgagca
     1921 gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg gaggcaaagg
     1981 cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc catcggtgcg tagaggaaca
     2041 gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg
     2101 gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc agagagagtt
     2161 tgaagccgag gccaacaatc atcaggccga aacctgccag caacagcggc tcgttgagca
     2221 taccgtcgcc aaggttttta cccaacgcga caaacgacag gtcgccagac tgcgcataca
     2281 ccagcgccat accaaacagc aggaaagaag acgctgcggc agaaaggatg gtgtatttga
     2341 tactggcttc cagtgaacgt ttctggcgga aagcgtaacc gaccaggcca aacagcggca
     2401 aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc gccagcagga
     2461 tcccgcccag cgcggcaatt aacaccaaca ggtagaactc gtccttgttg tcgttatagc
     2521 cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat accagcccgg
     2581 tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata gcgcctgcct
     2641 ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag agcgtagcgt
     2701 tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc gtcaagccga
     2761 cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg gcgatttacg
     2821 gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc gcggagtgcg
     2881 aggtatccag aatcggctgc ggatagaagc cctgcagtac cagcagcacc accagcaaca
     2941 ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca atctcgcttt
     3001 tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat gcaaagacca
     3061 gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg aacagaatca
     3121 taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc gcaaagaaca
     3181 gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc
     3241 gggtatggat acgttcataa agctgaccac aaagaataaa cagacccgcc gccgacaagc
     3301 cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg gtgtagatag
     3361 caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg
     3421 tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc cacatagcaa
     3481 ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc agcaaaccgt
     3541 aagcggcagt tttcagcaag atccccgcga ggtcaacgga accggcggtc ggagcctggg
     3601 agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact gcgaaggcga
     3661 tgaagaagcc cagcattaac aggtattccg caccactgga cattggcgta ttcagcagct
     3721 cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc agcgccagga
     3781 tggcaatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg gttgccgccg
     3841 tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg aagtacatcg
     3901 gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca aggaacacgc
     3961 cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga tatttttcga
     4021 tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc acgaccatca
     4081 gcagcgacag cccgtcaatg gcgagatgaa tagagatacc caaacgcggg atccacggca
     4141 tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa taaccgccct
     4201 gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc gcgatccagc
     4261 gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca ataaagggaa
     4321 ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt cccgtcagga
     4381 ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg tagtgtcgga
     4441 tgcaccgcga aggcggctta tccgacctac gttcttatat gcccaggggc aatcccacaa
     4501 tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc gatgctcatg
     4561 gatgccacat accagcgcag atagccgttc tcacttaaca gcagaccttt acctgcaaag
     4621 cgggaaagga cagccggaat gttcatcatt gagttcagcg gatcgcgttt cagcaaccag
     4681 gcaataccca ggaacggcct gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg
     4741 ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga ggtcaccaga
     4801 gtacgtttac ccagccacag ccaagctgcc agcagaatgc cgacaatcgc gaccacgcca
     4861 gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg
     4921 ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc
     4981 agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg
     5041 aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc gaccagacct
     5101 gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc atccttactg
     5161 aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac caggaagcag
     5221 agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga
     5281 tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa gaacgcgtga
     5341 gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag gaacatgtag
     5401 ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg taccagcgcg
     5461 gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatgc ccaccagatg cagaatttcc
     5521 ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc ggttaccatg
     5581 gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat
     5641 gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat cagcgtcgcc
     5701 cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac catttcgcgg
     5761 aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag gaaaacgtca
     5821 cccacacggg tcacgacgaa cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa
     5881 tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag gtacatcagc
     5941 agcaggttgt cggcaagcac cagaactacc atgctggcga tgaacaggtt ggtgtaggcg
     6001 aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat gtgaataagg
     6061 aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag caccaggtta
     6121 aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct gtatgcctgc
     6181 tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc caggcccaca
     6241 gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga gaacgccagc
     6301 aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat ccgcgcatct
     6361 cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc agcagcgcaa
     6421 ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc acctgaccgt
     6481 cggtctgacc ccagtagctt ccggcgacaa caaaggccag cgcggaggcg ttaatcatga
     6541 tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg gttaagccaa
     6601 gaatgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg cgtgctcctc
     6661 cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg cacgctcttc
     6721 acgaccgacg tggaaggcca caaccagacc tgcgagcagc agcatagaag ccagttccac
     6781 cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga ttggcgtacc
     6841 gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca gcatgatggc
     6901 cgacaaaatt gccggaccaa tccacacctg cggtttcagc cactggcgtt cctgttcgat
     6961 ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca taatggcacc
     7021 cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cccagtgaga agaacacccc
     7081 ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat tggtatgggt
     7141 gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag cgaactccat
     7201 tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc gttctctgct
     7261 tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt atattccggg
     7321 tatttgcccg gaccggagat cagcagatcc tctttttcgt aaaccagatc ctggcgcttg
     7381 tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca ggcttcttcg
     7441 cacagaccac agaaaatgca gcgtgagaag ttgatgcgga aaaattccgg ataccagcga
     7501 ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc taccgcgcag
     7561 aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac gatacgacca
     7621 cgataacggg gcggcagata gatcggctct tccgggtaca ttcgcgtttc gcgtttggcg
     7681 aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc tactaacaat
     7741 tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg acagccgccg
     7801 ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag gacattacct
     7861 ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc ataaagaacg
     7921 cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag ccaccgaaga
     7981 acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc acgaagaaca
     8041 gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc tgctcggctt
     8101 ccggctggtc aaacgggtga cggtgacata ccgccacgcc cgcgatggca aaggtaataa
     8161 aaccaaagaa ttgcgggata acgttccaca catgcgcctg gctgttgacg atgtcggtca
     8221 tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg aacacttcgt
     8281 agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa cgagtatttg ttgttacttg
     8341 accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg aagaacaaaa
     8401 tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatc gcaaaggcca
     8461 gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga tccgagaatt
     8521 tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc tggagcgaac
     8581 cgccccagcc aacacggtta ggtccgtaac ggttctggaa cagacccagc aggcgacgtt
     8641 cgccaaagct catgaatgcc ccgcaggtga caaccaccag caggatcacc accgctttga
     8701 ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt gtgcctcctt
     8761 gagatcctca agatgcgcgc cagccagcac cggagcaata ccggacatac ccatcggcaa
     8821 gcccacctgc cctgccgtca gcccttcagc gatttcaacc ggcagcgtga ccgtattgcc
     8881 atcgtaacta aaggagacgt gtgtacctgc gttcacacct aacttcgcgg catccgctgg
     8941 gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac gctgtgacaa
     9001 ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc catttcccgt cctgcggctg
     9061 gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt tcgctggttt caaacagacg
     9121 cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt tgttccacgc
     9181 ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt gcgcagtcgg
     9241 ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg gctgacgcgg
     9301 ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg
     9361 ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt tgatacctgc
     9421 cagttccggg attttcgcca caacagcgtc aatcacatgg tcgagctgcg tccagtccac
     9481 ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag cgccagcttt ccagcatgac
     9541 agttttgctg tcgtaataag caggatcgta aacctggaag aaacgttggg cgcggccttc
     9601 gttgttgatc accgtaccgt cgctttcagc aaaactggca gcagaaagta ccagatgggc
     9661 gttttccata atcgctgtgc gttgatgatc aaccaccatc accagcggcg ctttagccag
     9721 cgcagcattc acgcgggtag cggaagcgtg acgatgcaga tcgttttcca gcaccaccac
     9781 cgcgtcggcg cgtccggttt ccagttcggt caacgcttct tcaagcgaac cgccacccat
     9841 aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa cgtcagcgcc
     9901 gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta tgctaccggc
     9961 gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca cgatgacgtc
    10021 gattttgctt tgcagctcag gttcgatacc gtcaaccgct ggcgcagagt tatccagcgc
    10081 atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc
    10141 cgcgatatca tccagacggg tgtcatcaac gttggtaaca aacagcggat gcttcgcacg
    10201 ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg ctgccgccat
    10261 ttcgcgcgct ttacctttca cagcctgacg cactgccagc gcgacgcgcg cgccggtctg
    10321 ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg
    10381 agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt cctgctcacc
    10441 gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg caaagttgct
    10501 ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac gcagaatatc
    10561 tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg
    10621 tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac gaccacggtc
    10681 gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc
    10741 gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga tgctcggcgc
    10801 aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc
    10861 ggtcgggcaa atttcgacca ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg
    10921 gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc agatctgtac cgtcagcgta
    10981 atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt cgtgagagat
    11041 gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc gacggaagct
    11101 gtgtccggtc atcacagtca tatcctgaag atggcagtta ccgccttctt cacataccgg
    11161 acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga actgtttcgc
    11221 ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac aggacatcac
    11281 caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact ggcggcaagc
    11341 accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc ccagagacag
    11401 acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt ctacatgaat
    11461 tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa tcgaaattcg
    11521 gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg gtattgctga
    11581 acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg ctctgtaacg
    11641 gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag aatcgacaca
    11701 gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc gcacgcagaa
    11761 ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag gactcacggg
    11821 cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca
    11881 tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat tccatcggca
    11941 gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc caggctttaa
    12001 atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc
    12061 cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc atcagcttgg
    12121 tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta gcgaggatcg
    12181 ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc catacgccgg
    12241 aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc agggagttga
    12301 ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg aacagttcga
    12361 agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg gcttcggcaa
    12421 tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag atgtagccac
    12481 ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg tgcggcagtt
    12541 gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg ttacacagca
    12601 ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg ccagtcgaaa
    12661 agcccgctcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg atttcgtccg
    12721 gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt
    12781 ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc gtttcgggag
    12841 tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt ccggggtcag
    12901 atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt tccccaggca
    12961 gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt tgatgttcag
    13021 ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga ccacgctgtc
    13081 gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt agaacgttgc
    13141 cacaccttcg acgtcgcttg ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc
    13201 atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg acgccgcacg
    13261 cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg cactcagctc
    13321 aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac atctgacata
    13381 acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc
    13441 gccggaattt gctgcaaatg cgcataactc ggcgtgcgga tacgagtgcg ataactcatg
    13501 gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc aatcatctgg
    13561 aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg ggtgatcagg
    13621 gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct
    13681 ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag actctggcgc
    13741 agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc accaaccggg
    13801 atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac gtcgaagtcg
    13861 atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca
    13921 taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc cgctttctcg
    13981 taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcaggcg atcccagccg
    14041 cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat acggaaaccc
    14101 gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc
    14161 gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct gttgatacgg
    14221 aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc
    14281 agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag gtattcaata
    14341 cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt ctccgcacca
    14401 cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc gagttgtaaa
    14461 acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag gaacatgaag
    14521 tcctcgtttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt cagggcttcc
    14581 atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc
    14641 gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat gcggcgcagg
    14701 ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc ataccagtta
    14761 gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc
    14821 ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc
    14881 gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac
    14941 agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag taattgttca
    15001 cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc
    15061 ccaaaacggt tgcgcagttc gccaatcacc ggatcatcaa gatgatcacg ggtctgccag
    15121 gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga
    15181 ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa aatccctttg
    15241 ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca
    15301 ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg atcgccaacc
    15361 acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat cagtgcctgc
    15421 atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg gatgaattta
    15481 tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc acaggcaccc
    15541 attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat aaccggtgcc
    15601 attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc
    15661 aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt caccatctca
    15721 acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg accccagtta
    15781 accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc cagagggtcg
    15841 gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg atctatgcgg
    15901 gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc gatactgttc
    15961 gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata
    16021 cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc ttccacaaag
    16081 cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag cgcttcaacg
    16141 tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg taagcgggcg
    16201 gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga
    16261 ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc aacgataaga
    16321 aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat actcattgct
    16381 tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg ccacatcgat
    16441 tcatggggag gaataaaaaa aaccttacaa tcactgtaga aattctttta tacagctaat
    16501 tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa gtttcttcac
    16561 attagtttac ataatatcaa caccattagc atttaatgcc ctttcacgcc agatccttga
    16621 cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata
    16681 cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag tattttcttg
    16741 atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt acgtattttt
    16801 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgttcgg gacgtgaatc
    16861 tctggtgatt gaaaaatgaa cagttttgta cgttctgcac tatgcgagga aggattttta
    16921 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttac actattgtct
    16981 caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc
    17041 cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg
    17101 atagatcacc tgggccagct cattattact ggacgggtca tagcacagca aatattcggt
    17161 atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat
    17221 ttcaatcggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag
    17281 cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg tcgccagcac
    17341 catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg
    17401 caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt
    17461 aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc
    17521 tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact
    17581 cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt
    17641 taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag
    17701 tattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc
    17761 gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac
    17821 ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac
    17881 agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat
    17941 catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac
    18001 aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta
    18061 aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag
    18121 tagatcacag aatatatctt cagggatcgc atatctatta agttactcac tcttttctat
    18181 ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag
    18241 catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg
    18301 gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg
    18361 aattatctga tg
//
LOCUS       DAZPLM010000070        18214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPLM010000070 DAZPLM010000000
VERSION     DAZPLM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.070"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHVC7_002051"
     CDS             15..1091
                     /locus_tag="ACHVC7_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2116101.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHVC7_002052"
     CDS             complement(1484..1825)
                     /locus_tag="ACHVC7_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR2116102.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHVC7_002053"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHVC7_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR2116103.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHVC7_002054"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHVC7_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR2116104.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHVC7_002055"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHVC7_002055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR2116105.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHVC7_002056"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHVC7_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR2116106.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHVC7_002057"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHVC7_002057"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR2116107.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHVC7_002058"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHVC7_002058"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR2116108.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHVC7_002059"
     CDS             complement(7167..7826)
                     /locus_tag="ACHVC7_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR2116109.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHVC7_002060"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHVC7_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR2116110.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHVC7_002061"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHVC7_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR2116111.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHVC7_002062"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHVC7_002062"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR2116112.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHVC7_002063"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHVC7_002063"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR2116113.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHVC7_002064"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHVC7_002064"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR2116114.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHVC7_002065"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHVC7_002065"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR2116115.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHVC7_002066"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHVC7_002066"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR2116116.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHVC7_002067"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHVC7_002067"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR2116117.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHVC7_002068"
     CDS             complement(17270..18112)
                     /locus_tag="ACHVC7_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR2116118.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4670 a         4574 c         4393 g         4577 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZPLM010000071        18049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPLM010000071 DAZPLM010000000
VERSION     DAZPLM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.071"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            271..576
                     /locus_tag="ACHVC7_002069"
     CDS             271..576
                     /locus_tag="ACHVC7_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116119.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            683..1327
                     /gene="gfcB"
                     /locus_tag="ACHVC7_002070"
     CDS             683..1327
                     /gene="gfcB"
                     /locus_tag="ACHVC7_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR2116120.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1324..2070
                     /locus_tag="ACHVC7_002071"
     CDS             1324..2070
                     /locus_tag="ACHVC7_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR2116121.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2070..4166
                     /locus_tag="ACHVC7_002072"
     CDS             2070..4166
                     /locus_tag="ACHVC7_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR2116122.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4212..5351
                     /locus_tag="ACHVC7_002073"
     CDS             4212..5351
                     /locus_tag="ACHVC7_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR2116123.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5339..5785
                     /gene="etp"
                     /locus_tag="ACHVC7_002074"
                     /gene_synonym="yccY"
     CDS             5339..5785
                     /gene="etp"
                     /locus_tag="ACHVC7_002074"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR2116124.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5805..7985
                     /gene="etk"
                     /locus_tag="ACHVC7_002075"
     CDS             5805..7985
                     /gene="etk"
                     /locus_tag="ACHVC7_002075"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR2116125.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8100..9398)
                     /gene="appA"
                     /locus_tag="ACHVC7_002076"
     CDS             complement(8100..9398)
                     /gene="appA"
                     /locus_tag="ACHVC7_002076"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR2116126.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9478..9570)
                     /gene="cbdX"
                     /locus_tag="ACHVC7_002077"
     CDS             complement(9478..9570)
                     /gene="cbdX"
                     /locus_tag="ACHVC7_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR2116127.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9583..10719)
                     /gene="appB"
                     /locus_tag="ACHVC7_002078"
     CDS             complement(9583..10719)
                     /gene="appB"
                     /locus_tag="ACHVC7_002078"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR2116128.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10731..12275)
                     /gene="appC"
                     /locus_tag="ACHVC7_002079"
     CDS             complement(10731..12275)
                     /gene="appC"
                     /locus_tag="ACHVC7_002079"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR2116129.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12409..13266)
                     /locus_tag="ACHVC7_002080"
     CDS             complement(12409..13266)
                     /locus_tag="ACHVC7_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR2116130.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13323..13661)
                     /gene="hyaE"
                     /locus_tag="ACHVC7_002081"
     CDS             complement(13323..13661)
                     /gene="hyaE"
                     /locus_tag="ACHVC7_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR2116131.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13658..14245)
                     /gene="hyaD"
                     /locus_tag="ACHVC7_002082"
     CDS             complement(13658..14245)
                     /gene="hyaD"
                     /locus_tag="ACHVC7_002082"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR2116132.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14242..14948)
                     /gene="hyaC"
                     /locus_tag="ACHVC7_002083"
                     /pseudo
     CDS             complement(14242..14948)
                     /gene="hyaC"
                     /locus_tag="ACHVC7_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(14967..16760)
                     /gene="hyaB"
                     /locus_tag="ACHVC7_002084"
     CDS             complement(14967..16760)
                     /gene="hyaB"
                     /locus_tag="ACHVC7_002084"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR2116133.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16757..17875)
                     /gene="hyaA"
                     /locus_tag="ACHVC7_002085"
     CDS             complement(16757..17875)
                     /gene="hyaA"
                     /locus_tag="ACHVC7_002085"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR2116134.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4409 a         4814 c         4695 g         4131 t
ORIGIN      
        1 tattaccaac aataattaat agctttcgta acaatttcct tatctttaat ttgtattttg
       61 ctaagattta tcttatgctc acaagtcgta tttccagagg ggatcgttaa taatcttata
      121 tttccctctg cgcacctttc ctacccgcag tatcagtata tagtgcaagc ggtatgagga
      181 aagggagatt tcacaaggat gttatactca aagtagacaa tcctgatctc aagcgtacgt
      241 attgtccatg cgatgaataa aggaaaagtt atgaaacaca agctttctgc aatccttatg
      301 gccttcatgt tgacgacgcc agctgcgttt gctgcctctg aagcaacaaa cgggaccgag
      361 gcaacgacgg gtactactgg caccacaacg accactaccg gcgcaaccac gactgctact
      421 accactggtg gcgtggctgc tggcgctgtc ggcacggcga cagttgtcgg tgtggcaacg
      481 gctgttggtg tggcgacgtt ggcagttgtt gcagcaaatg acagtggcga tggcggttct
      541 cataacacct caaccaccac gtccactaca cgctaatact gctttaacgc gtcgaaatta
      601 aagttaaatg atacccggtt gcgatattcg taccgggctt tagagatttg cattttttta
      661 attaaccttt caaggattaa aggtgcgccc tcttatttta tcgattttcg cactatttct
      721 tgcgggatgt acgcacagcc agcaaagtat ggtcgataca tttcgcgcca gccttttcga
      781 taatcaggat atcaccgtag cggatcagca gatccaggcg ttgccttatt ccacgatgta
      841 tttacgcctt aatgaagggc aacgaatctt tgtggtactg ggatatatag aacaagaaca
      901 aagcaaatgg ttatcccagg ataacgccat gctggttacc cacaatggac gtcttttaaa
      961 aaccgtcaaa cttaataata atctgctgga agtgacaaat tccgggcagg accctctgcg
     1021 taacgcgctg gcaataaaag atggcagccg ctggacgcgc gatattctct ggagtgaaga
     1081 caaccatttt cgctctgcga ccctgagttc tactttttcc tttgctggat tagagacgct
     1141 gaatattgcg ggtcgcaatg tgctgtgtaa tgtctggcag gaagaggtga cttccacgcg
     1201 gccagaaaaa cagtggcaaa acacattctg ggtcgattcg gctactggcc aggttcgtca
     1261 aagtcggcaa atgttaggcg caggggttat tcccgtagaa atgacgtttc ttaaacccgc
     1321 accatgaata aattacagtc gtatttcatt gccagcgtac tttacgtaat gacaccccat
     1381 gcctttgcgc aaggaacggt gactatttat ctgcctggcg aacaacagac actttccgtt
     1441 ggccccatgg aaaatgttgt ccagttggtg acacaaccgc aactgcgcga tcgcctctgg
     1501 tggcctggtg ccttgctgac cgactctgcg gcgaaagcta aagcgcttaa agactaccaa
     1561 catgtcatgg cacagcttgc ttcctgggaa gcagaagctg acgacgatgt ggcggcaacg
     1621 attaaatccg tgcgccagca gttactcaat ctcaatatca ctggtcgctt accggtcaaa
     1681 ctggaccctg attttgtacg agtggatgag aacagcaatc ccccgttggt cggggactat
     1741 acgctctata ccgtacagcg tccggtgact attactctgc tgggtgcggt ttctggggcc
     1801 gggcaattgc cctggcaggc aggccgtagc gttaccgatt atctgcaaga tcacccccgc
     1861 cttgcaggcg cggacaagaa taacgttatg gtgattacgc ctgagggtga gactgtcgtc
     1921 gcccctgttg ccctgtggaa taaaagacat gtggaaccac cgcctggcag ccagctttgg
     1981 ttgggctttt ctgcgcatgt actgcccgaa aaatatgctg atttaaatga ccagattgtg
     2041 tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa ttcttatctt ttaagctgcc
     2101 tggccattgc cgtctccagt gcctgtcatg ctgaagtatt aacctacccg gatccgctgg
     2161 gttcgtcgca atcagacttt ggcggcacag gattgttgca gatgccaaat gcgcgcatcg
     2221 caccggaagg tgaattcagc gtcaactacc gggataacga tcaataccgg ttctactcca
     2281 cctccgtggc gctgttccca tggctggaag gcaccattcg ttatacggat gtgcgcacac
     2341 gcaaatatag ccagtgggaa gatttcagcg gcgatcagtc atacaaagac aaatcattcg
     2401 attttaaact tcgcctgtgg gaagaaggtt actggctacc gcaagtggcg tttggtaaac
     2461 gtgatattgc tggtacgggt ctgtttgacg gtgagtatct ggtggccagc aagcaagcgg
     2521 ggccatttga tttcaccctc gggatggcat ggggctacgc cggtaatgcg ggcaatatta
     2581 ccaacccgtt ttgccgggtg agcgataaat attgtcatcg cgcagagtct cacgatgcgg
     2641 gcgatatcag ctttagcgat atctttcgtg gcccggcttc catctttggc ggcattgagt
     2701 atcaaacgcc gtggaatccc ctgcgtctga aactcgaata cgatggcaac aattaccaga
     2761 atgatttcgc tggcaaactg cctcaggcaa gccatttcaa cgtcggcgca gtttatcgcg
     2821 ctgccagctg ggcagatctc aacctgagtt atgaacgcgg taacacgttg atgtttggct
     2881 tcacgttacg gaccaatttc aacgatctgc gccctgccct gcgcgatacg ccaaaaccgg
     2941 catatcaacc tgcgcctgaa tctgaaggat tgcagtacac cacagtagcg aaccaactta
     3001 ccgcgctgaa gtacaacgca ggttttgaag caccggaaat tcagctgcgc gataagacgc
     3061 tgtatatgtc tggtcaacaa tacaaatacc gtgattctcg cgaagcggtc gatcgtgcca
     3121 accggattct ggtgaataac ctgccgcaag gcgttgagaa gattagcgtg acgcaaaagc
     3181 gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc cagcctgcgc aaacagctgg
     3241 ctggtacagc gcctggtcaa tcagagcaac ttcaacaaca acgtgttgaa gcagaagatc
     3301 tttctgcctt tggtcggggc taccgtattc gtgaagatcg ctttagctac tctttcaacc
     3361 caacactttc acagtcgctg ggcggcccgg aagatttcta tatgttccag ctggggctga
     3421 tgtccagtgc ccgctactgg tttaccgacc acctgctgct tgatggcggt attttcacca
     3481 atatttacaa caactacgac aagtttaagt cttcgcagtt gcccgcggac tctaccctgc
     3541 cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga cgtttatctc aacaacttgc
     3601 aggcgaacta ctttgccgac ttaggcaatg gtttctatgg ccaggtgtat ggcggttatc
     3661 tggaaacgat gtacgccggt gtcggttccg agctgcttta tcgcccgcta gatgccagct
     3721 gggcgctggg tgtggacgtt aactacgtga agcaacgtga ctgggacaac atgatgcgct
     3781 tcaccgatta ttccacgcca actggtttcg tgacggctta ctggaacccg ccgacgctca
     3841 atggcgtact gatgaaactt agcgttgggc aatatctggc aaaagataaa ggggcaacga
     3901 tcgacgtcgc caaacgcttt gacagcggcg tggcggtagg ggtatgggcg gcaatcagta
     3961 acgtatctaa agatgactac ggcgaaggcg gctttagtaa aggtttttat atctcgattc
     4021 cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc ggtggtttcg tggacaccat
     4081 tgacgcgtga tggtggacaa atgctgtcac gcaaatacca gctctatcca atgacggcag
     4141 agcgagaagt accggttgga caataacctg acgtaattca taacattaca ttgactgata
     4201 aatataaacc aatgaaaaag aacattttta agttttcggt actgacattg gctgttctgt
     4261 ctttgacagc atgtaccctt gttcctggtc agaatttatc gaccagtaac aaggatgtca
     4321 ttgaactacc tgacaatcag tatgacctgg acaaaatggt caatatctat cccgtcacgc
     4381 caggattgat tgatcagttg cgcgccaaac cgattatgtc gcaggctaac ccggagctgg
     4441 agcagcaaat cgcaaactat gaatatcgta tcgggattgg tgatgtgttg atggttacgg
     4501 tctgggatca cccggaacta accacccccg cagggcaata ccgtagcgcc agtgataccg
     4561 gcaactgggt taacgccgat ggtgcgattt tctaccccta tattggtcgc ctgaaagtgg
     4621 ccgggaaaac acttacccag gtacgcaacg agattacagc gcgtctcgat tcagtcattg
     4681 aaagcccgca agtcgatgtc agcgtcgcgg cgttccgatc gcaaaaagcg tacgtcaccg
     4741 gcgaagtcag caaatcgggt cagcagccga ttaccaatat tcctctgacc atcatggatg
     4801 cgattaacgc cgcgggcggt ctgaccgcag atgcagactg gcgcaacgtg gtgctcaccc
     4861 agaatggcgt aaagaccaaa gtgaacctct atgcgctgat gcagcgtggc gacttgcgcc
     4921 agaacaaact gctgcacccg ggcgatattt tatttattcc gcgcaacgat gatctgaaag
     4981 tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa aatggaccgc agcggtatga
     5041 ccctggcgga agcgctgggt aacgctgagg gtatgaatca ggatgtcgct gatgcgacgg
     5101 ggatttttgt tattcgtgca acgcaaaaca agcagaatgg caaaatcgcc aatatttatc
     5161 agctgaatgc gaaggacgca tcggcaatga tactcggaac ggaattccag cttgaacctt
     5221 acgacattgt ttatgtcact accgcgccgc tggctcgttg gaaccgtgtg atttctctgc
     5281 tggtaccgac gatttccggg gtgcatgacc tgacagagac ttcacggtgg attcaaacat
     5341 ggcccaacta aaatttaact caatcctggt ggtttgtacc ggcaacattt gccgttcgcc
     5401 cattggcgag cgcttgctgc gtaaacgtct gccgggcgta aaagtcaaat cggcaggtgt
     5461 tcatggtctg ataaaacacc ctgcagatgc gacagcggca gatgttgccg ctaatcatgg
     5521 cgtatctctt gaaggacatg ccggacgtaa gctcactgct gagatggcac gaaattacga
     5581 tctgattctg gcaatggagt cggaacatat tgctcaggtt accgcaattg ctcccgaggt
     5641 ccgcgggaag acaatgctgt ttgggcaatg gctggaacag aaagagatcc cggatcccta
     5701 tcgtaaaagt caggacgcat ttgaacatgt ctacggtatg ttggagcgcg ccagtcagga
     5761 atgggcgaag cgcctcagcc gataatttga gtttataaaa tacgatgaca actaaaaata
     5821 tgaatacgcc accaggcagc actcaggaaa atgagatcga tctgcttcgt ctggtcggcg
     5881 agttatggga tcaccgtaag tttattatca gcgtgaccgc gttattcacg ctgatcgctg
     5941 tcgcttactc gctgttaagc acaccaattt atcaggcaga tactctggtc caggttgagc
     6001 aaaaacaggg caacgccatt ctcagcggcc tgagcgatat gatccctaac tcatcgcccg
     6061 agtctgcacc ggagatccaa ctgctgcaat cgcgcatgat tctcggtaaa accattgctg
     6121 aactgaatct gcgcgacata gttgagcaga agtattttcc gattgtgggt cgcggctggg
     6181 cgagattaac caaagaaaaa ccaggtgagc tggcgatcag ctggatgcat attccacaac
     6241 tgaatggtca ggatcagcaa ctgacactca cggttgggga aaacggccac tatacactgg
     6301 aaggtgaaga gttcaccgtc aatggtatgg tcggccagcg tctggaaaaa gatggcgttg
     6361 cgctgactat cgcggacatt aaggccaaac caggaacaca gtttgtcctg agccagcgta
     6421 ccgaactgga agcgattaac gcattgcagg aaacctttac cgttagcgaa cgcagtaaag
     6481 aaagcgggat gctggaactt accatgactg gtgatgatcc ccagttgatt actcgtattc
     6541 tgaacagcat cgctaacaac tatttgcaac agaatatcgc tcgccaggcg gcgcaggatt
     6601 cacaaagcct tgaattctta cagcgccagt tacctgaagt gcgcagcgag ctggaccagg
     6661 cggaagaaaa actcaacgtt tatcgccagc agcgcgattc ggttgacctt aacctggaag
     6721 ccaaagccgt tcttgagcag attgtgaacg ttgataatca actcaatgag ctgaccttcc
     6781 gcgaggcaga gatctcccag ctgtataaga aagatcaccc aacttatcgt gcgctgctgg
     6841 aaaaacgcca gacgctggag caagaacgca aacgcctgaa taagcgggta tcggcaatgc
     6901 cttccaccca acaggaagtg ttgcgtttaa gtcgtgacgt agaagcgggc cgtgcggtat
     6961 atctgcaatt acttaaccgc cagcaggagt tgagtatttc gaaatccagt gccattggta
     7021 acgtgcggat tatcgacccg gcagtcactc agccgcagcc agtgaaaccg aaaaaagcgt
     7081 tgaatgtggt gcttggtttt attcttggcc tgtttatttc tgtgggtgcc gtgctggcgc
     7141 gtgcgatgtt gcgtcgtggt gtagaagccc cggaacaact ggaagagcac ggcatcagcg
     7201 tttatgccac tatcccaatg tccgagtggc tggataaacg cacccgtctg cgtaagaaaa
     7261 atttattttc taatcagcag cgccatcgta ctaaaaatat ccccttcctg gcggtggata
     7321 acccggcgga ttctgctgtg gaagccgtac gtgcgctacg aaccagtctg catttcgcta
     7381 tgatggagac ggagaataac attctgatga tcaccggtgc gacgccagac agtggtaaaa
     7441 cgtttgtcag ttcaactctg gcagcggtga tcgcccagtc cgatcaaaaa gtgttattta
     7501 ttgatgccga cttacgccgt ggttattcgc ataacctgtt taccgtgagt aatgaacatg
     7561 gcttgtcgga atatctggca ggtaaagatg agctcaacaa agtgatccag cattttggca
     7621 aaggaggctt tgatgtgatt actcgcggtc aggtgccacc taacccgtct gaactgctga
     7681 tgcgcgatcg gatgcgtcaa ttactggaat gggcgaacga ccattacgat ctggtgattg
     7741 tcgatacgcc gccgatgctg gcggtgagtg atgccgcggt cgtggggcgt tctgttggca
     7801 ccagcctgct ggttgcgcgt tttggcttga acaccgccaa agaggtgagt ttgtcaatgc
     7861 agcgtctgga acaggcaggc gtcaatatta aaggcgctat cctcaatggt gtgattaaac
     7921 gcgccagcac cgcttacagt tacggctata actattacgg ttatagttac tccgagaaag
     7981 agtaaaactg aaagtggccg gatgcggcgt gaacgccttt tccggtctac gcattagaca
     8041 gttcttcgtt tgtcatcagc ctcagagcat tcaggtaact gaatgctctt ttttatgcat
     8101 tacaaactgc acgccggtat gcgtgcttca ttcacgattt gcgtaaaacc tgccaacgaa
     8161 cacatgccct gcgcatttcg ctcttcacat cctgccaggg tcagtttcac ctctccgggc
     8221 ggcgtattta atgacagcgg cgttttatca cgcatctgct gtaaagtctg gaagaccagc
     8281 gaaacctgaa tccactggct gttatcgctt agccgacgcc agcgttcaaa caccagttca
     8341 ccacctggcg gcgtgttatc cggctgaccg ggaagcgtcc agttgagctc cagtgcgccg
     8401 ccgagatttg ccagattagt atcgtgtccg gcgataaaca gcactgaagt gggtaatgtc
     8461 acaccatacg cctgtttttg cggtggatgg ggcgtcaacg ctgtcttgat caaatctaat
     8521 aacggggtgg cgcggctgcg ggcaacctct ggcgtgcgtt gtagcaaata aaattgcgcg
     8581 ttatgcaaac ttagcaaggt gttccactgg tgtgaatcgg tgatccttcc ccaccccggc
     8641 tccggcattc cctgtgcttg ttgcaggaga aatatctccg tcagcattga tgcgaggctt
     8701 accgcaccgg ttaatgagac attgtcggcg ctcaccttga gttccgatgg taatgcctgc
     8761 gttaatgaac agctttcgtc ctgtttctca cgtttaaggc acaagtttga ttgcggaaaa
     8821 ttaagcaccc gttccagttc gcgaaacgcc gtttgccgat gcccggtaaa gtcagcaatt
     8881 gaccctcctg ccctgctgag gatcgcgtca gtcacgttcg cgttatccag ttggcaaacg
     8941 ccagttttta gaggattaaa taacggatcg ggactggacg tatcagcctg ggtatgtacg
     9001 gttattgcac agtcaggtgc cagcccggcg gcgaaggctt cgcctgtttt acgggtacgc
     9061 tcgtcgacat cagcaataat cgcgacctga ccagactgcg ggcagccctt tttcgccagc
     9121 aatccgtcgg ctaccagacg ctggcgttgg taatgtccga gataggcgat tagctcacca
     9181 ccgcgcggtg tcagccaacc cagttttacc ggccaggttg gccatgcgtc tggggtgaca
     9241 tcctgcatca gttgcgtggc cttggttgga gcacgcacac catgacgact gacaatcacc
     9301 acactttcca gcttcagctc cggctcactc tgagcgaatg cagattgcgg ggttaacgga
     9361 atcagaagag ataaaaatgg gattaagatc gctttcatcg atatgtttcc gcttttcata
     9421 tctgacatta ttgattgcct gatgcgatgc taatgcgccg catcaggcct acgttcgtta
     9481 acttttcaga cgcgggtcca gccataccaa cacaagggtg gagagcgaac acatcaacaa
     9541 aatgccgacg aaccaaagta aataccacat tgttcctgct ccttagtaca actcgttttc
     9601 gttacggcgg agagtttctg ttgtcatgcg tccccacatt ttgtagtagc tccagagagt
     9661 gtagagcaac acaatgggca aaaatatcag cacgattacc aacataatgc tcagcgtcag
     9721 ctgactggaa gtgctgtccc acaacgtcag gctggagatt ggactcacgc ttgacggcat
     9781 gacaaagggg aacagcgtga tgcctgccgt gaaaatcacg ccaaattgca tcaatgacgc
     9841 cattaaaaat ccccaacccg ggcgaccacg ataaatcgcc atcaccgtca gcaatgggca
     9901 gaagaacccc agcagcggga agatccacaa aacgggcgat tcgacgaaat tattcatcca
     9961 ggcaccaggt agcactgcca ccagtttcat taacggattg gaaggaccgt tagcatcctg
    10021 ggcgagcagt acaaagccat caataccgac ccacagccag taacccgcca gcaaaaagca
    10081 cagcatcacc aacagtgcag cgcgtttggt ggccagctgt gaacgcagat gaatcacacc
    10141 aacagttttc agttgtaacc agacgccacc ttgcaaaatc accatcccaa ggctgagcaa
    10201 tccgcacaat aaagggaatg gcgtcagcag ttgccagaag ctaccgagat actccacgcg
    10261 taattgcggt gtgaaggcaa acggcacgcc gagcaacaag ttgccgaagg cgataccgaa
    10321 gactaccggc ggcaccagac tgccgatgac cagaccggcg tcccacattt tacgccagcg
    10381 tgcatcggcg atttttccgc gataatcaaa ggccagcggg cggaagaaca gtgagcacag
    10441 caccaggatc atcgccacat aaaagccgga aaacgccgct gcatacactc tgggccaggc
    10501 ggcaaataat gccccaccag cgaggatcaa ccagacctgg ttgccttccc agtgtgcacc
    10561 aacgctgttt atcactatcc ggcgttcatc atcattacgc gccaccagcg gcagcagaca
    10621 gccgatcccc atgtcaaatc cgtcggagat cataaagacc accaggatca cgccaatcag
    10681 cagccaccag atgaagcgca atgtttcata atcaaacatg attttcccct ttacccctgt
    10741 tgctgcgtcg gttgttcact ctgcatcgcg ctcggcccca gacgggcata tttctgcatc
    10801 aggtagactt cggcgattaa gaacagggtg taaagcccta cgatcatgat cagtgagaaa
    10861 gccagttgtc ctgtggttaa agcggagtgc gcggagtatg tcggtaagat gtcctgtatc
    10921 gcccacggct gacgaccaaa ctcggtcata aaccacccgg cttcaatcgc aatccacggc
    10981 aacggcaaac tccagagcgc cattttcagc acccagcgat gctggtcgat tttgccacgc
    11041 agcgtctgga caagcgcaat cagcatcacc agtagcggca gggaaccaca gcccaccatg
    11101 atgcggaaac tccagaatac cggcgcaacc tgaggtatcg cgccacgcat cgccgcctgg
    11161 tactgtgcgg ctgtgacatg attcatatcc ggcgcatagc gggagagcaa catgccgtag
    11221 cccaggtcac cttccagttc ccggaatgcc tgcaacacat gcggctcacg attgccttgc
    11281 gatatttcct gcattaacag ccaggccata cgtccgcgtt gcaagcgtgg gtaggtttca
    11341 gccatcaaat ttttcagacc cggcacggtt ttatctaatg agtgagtggc gaggatccct
    11401 aacagcgcgg gaattttgat ggcaaaggcg ttacgctctt gatcctgttc cggccaggca
    11461 accacatgga acggtgcagg agcaggttcc gtttgccact ccccttccat cgccgccagt
    11521 tttaccggtt gtacttgcgc gacttcatac gcagaactgt ctccgagttg tagcgtgccg
    11581 ataatcgcca gagtaccgaa gacggaaccg atggcaaacg agcgtaatgc aacatcgcgc
    11641 tcccgtccgc gcagtaaata ccaggcgctg atcgccataa taaacatggc cccggtcacg
    11701 tagcccgcca ttacggtgtg cacaaatttc acctggctga ccgggttaaa gaccagctcg
    11761 ctgaagctgg tcatctccat acgtagggtg tcgatatcaa aatgcgcacc ggtcgggtat
    11821 tgcatccaac cgttggcatt caatatccac aatgcagaga gatttgaacc gaacgccacc
    11881 agccacgtca ccagcaggtg ctggtattta ttcagccgct gccagccgaa gaagaaaagc
    11941 ccgacaaagg tggattcgag gaagaaggcc attaatgctt ccatcgccag cggtgcgcca
    12001 aaaatatcgc ccacatagtt ggaatagaat gaccagttag taccaaactg aaactccatg
    12061 gtcaggccgg tagccacgcc aagagcaaag ttgataccga agagcttacc ccagaagcgc
    12121 gtcatatcgc ggtagattgt tttgccggtg accacgtaaa tggtttccat gatagccagc
    12181 aaaaaaatca gccccagggt aaggggtaca aataaaaagt gatacagcgc ggtcagagca
    12241 aactgccagc gcgataaatc aatgacatcc cacatgcgca ctcctgtagg cgaagagaga
    12301 aaaataaccc gataatgtta gatctccctc tttatcctga aactctacga ccagaggtat
    12361 tcgccgattg cgatattcat aagactgaaa gcgatactta ccgtcttttt acgtcggtgc
    12421 agcttccgcc agccactgac atacctcgct aagccgctgc gcagagtcga ccaaatcttc
    12481 tggcgctgcc agcaccactt ccggtattgg gcagatttca taactttcca gtaaaggacc
    12541 ttttaaggtg tccgtacagc gtaaatgcca gacatggcgt aaccccgtgg cgttgatata
    12601 gctctcgcca tagccaatgg tacgaatctg aatatttccc ggcccacaga gacgtgagag
    12661 aaaaagccga tcagcctcgc tgatgggtaa ttgcgtcaga ttaatgctgt gcggctgcgc
    12721 gtcggggtta cgtacatgcg ccagtaactc atgcgccaac ggtaggccat tcatcaggcc
    12781 atcaatgggc ggcggtaaca gcgaatctgt cggcaagaga ttttgcgtgg ccgcctgcca
    12841 caacgccagc ggcgcgcagc cggcctccag tttgtcctcc agcaactttt cgccgcgacg
    12901 tttgcgcacc cgccataatc cgcagaagat cgcctcctgt atttcacttt cgctgtcgtc
    12961 agcctgctga atgcgcactg acacttcccc ttccccgaga agcgtattga taaagtgacg
    13021 atcgtcggcg ttgagattta acagatcgac tgccagcacc tcgcccaacg gcggctggcg
    13081 ttgacttagt tgatgttgta actcgtttaa cagcgcaatc acctgcgggc tgtgagcaca
    13141 ttgctccagg gccgccatac tcggttcatc attcacctga cagctgatcg gcagtggatt
    13201 catgctgaaa ctgtcatcgt tcggttgcgt tcctggcccc agcagatgga aaaaagtttc
    13261 gctcatgagg cacgctcctg ttgtggttcg acaagcccac gcatcaggtt tatcagttcc
    13321 gctcacgggt gaataccatt cagcacaccg cgatagtttc cgccggtaaa cactaatgtg
    13381 gcgggaaagc gaaagacgcc aaagcgatct ccgatggctt cgctctgttc aaggtcagca
    13441 atcgccacct gccatgtgta gccgggaaac tcgcgcagta attcgccaat cattaccgga
    13501 ttatcgctga cctctggcgt gcgtttcggg tcactgctta ataacaccac gccgtctggc
    13561 gcttgcgtaa gccagtcgtc aagacggaat tcactaaccg gcgtccagcc gcgcgccagc
    13621 attcgttgcc acaacgcatc aaatggcgtg tcgttgctca tccctgctcc tccagtgtca
    13681 tcctgtactg gcgcaagcga acgccttcgt aattttccat cgacagacag tcataattga
    13741 gacagcgcga ttcattagcc ggttgcggca caatacccca tgcagccagc tgcgccagcg
    13801 ccgcctgttc cgctgcgggc agttgctccc gtgccagttc gctcaggcgg ccgccgtagt
    13861 cgtcgagcat tgcgggttgc agaccgacga gggcaatatg tgctggcaga tgtccgcgga
    13921 tatctgccag cgccagcact tcggagaaac tgttctggtg caggctcatt ttcttcgcgc
    13981 tgagataagc cggaatacgt tctccggcat aggttcgcag cgttccaggt tccagcccgt
    14041 agtcaatggc atcgagaatc aacagatggc tggcgctttc gacatacccc agcaagttca
    14101 gtccctgagt accgccatcg acaatctcca catactcggg ccagtggtaa tgggcataca
    14161 accgttccgc cacccgcacg ccgaaacctt catcggccca cagcaggttg cccagcccca
    14221 ttaccacaac gcgttgctcg ctcatgaacg ctccttgtta cttattttgc caaatttgtg
    14281 gctacggtag ccgttgacca tggtggagat caccgtgtcg tcggacatga tgtcttcacg
    14341 cagcgccata tagacatgac cgatcacaaa cgcgccaatc agccacatcc ccagccgatg
    14401 ccagctgtga atgtccattg agttgcctcc cgtccagtag aaaaattcca ccacataacg
    14461 gaacggcgca aaaatagcgt actggctgtg ttcgctgtac agcgcaaagc cggtgatgat
    14521 cataaagacc gacatcagga aatagccgaa catcgccgcc tgggcgatgg ggttatggcc
    14581 tatatcggca ctcggacgtt ttgccagaac agataccagc ggatttcata ccacacgccc
    14641 tgccaccagc ttttacgcca taccggcacg ataaacagct cgcgggagta tcgattgcca
    14701 acaaaagccc agtagatccg catcagcaag accacggtaa aaaccatccc ggcgctgaag
    14761 tgaattaacc tgatgtagcc catatagaac agatacgtcg cctcgccgct gacggaaggt
    14821 agcggcttgc cgataaagta tccggtgacc atcaacaccg ccatgcataa caccgtcagc
    14881 cagtgccaga tgcggactgg cgcttcaaag acatagtggc tgacaacgtt gtcgcttttc
    14941 tgttgcatga tgattctcct tcgctgttaa cgcacctgca cggagatcag ctcgctaccg
    15001 tcgtcgccca gcacgtgtgt tgaacaggcg aggcacgggt caaagctgtg cagagtacgc
    15061 aggatctcca gcggttgctc ggggatcgcc attttggtgt tcatcagcgc cgcttcataa
    15121 gcgccaatct gccctttggg atcgcgcggg ctggcgttcc aggtggtcgg caccacgcac
    15181 tggtagagat caatcttgcc atcgcgaatg gcggcccagt ggcctaacgc cccgcgcggc
    15241 gcttcggtaa agccgacgcc acggcactct gtcggccagg ttgcaggttc ccatttttcc
    15301 gtggaggcgg tggcaagatt gccgtttttc aggttagtca tcagcttgtt gaagaaatat
    15361 tgcaacttac ctgcggccca ctgtgcttcg tgcgcgcggc acaaaatgcg gcccaacgtt
    15421 gactggatac cggaaagcgg caggttcagc gccgacatca tgcgatcgac cgactcaacg
    15481 gtcgcagcat cgcctttgtg ataagcgatt aacgtgcgtg ccagcggccc cacttccatc
    15541 gcgttaccgc gccagcgcgg cgctttgatc cacgagtagc gttcctgttc attcagctgc
    15601 tgaatgttgg tatcgctgcc tttgacatcg ccggggttgt accacgggtc ggtaatgcca
    15661 tcgaatggat gacgcccgac ctgatcgttg ggataacgat accaggcgtg gtcgacaaac
    15721 tcctgcacct gctgcggatc aaccaaatcc actggcagca cattgttgaa gtcgccgtta
    15781 atcaccgcgc cgccaggcat cagcagactt ttctcaccaa agtcgttggc aatatccggg
    15841 aacgcaccgt agctgaggac acatttatca gaaagacccg tgccgatttc gctccacggt
    15901 ttgttgaact gaccgatggc taaggcgtcg gggatcatca cgttgttaat gaagtccgcc
    15961 gtgcgggtga taatcgactg caccaggttc aggcgttcca tattgaccgc cccgaccgcg
    16021 ccgctttcgt caatgttaat ggcgcaaggc atcccgccga caatccagtt tggatgcggg
    16081 tttttaccgc caaagaccgc gtggattttg acaatttcac gctggaaatc gagagcttcg
    16141 agatagtggg caaagcccat caggttagct tctggcggca gtttgtactg cgggtgcccc
    16201 cagtagccat tgcggaagat ccccaactgc ccgccttcaa caaatttttt caggcggttt
    16261 tgtacgtcga agaaatagcc aggggatgat ttcggccaag aggagagact ttgcgccagt
    16321 tcggaggttt tccgcgggtc ggctttcagc gcatctaaca catcgatcca gtccatcccg
    16381 gcaagctgat agaagtgcac cagatgatcg tggcaccaga gcgttgccag cataatgttg
    16441 cggatgatat tggcgttgtc cggcacttta ataccgatag catcttcgat ggcgtaaacc
    16501 gaagccaggg cgtgtacgcc agtacagacg ccgcagatac gttcaacgaa cgcccacgca
    16561 tcgcgcgggt cgcgcccttg taggatgatc tccagcccgc gaaacatggt gccgcaggag
    16621 acggcattgg tgatcacatt ctgatcgtta atattcactt cgcagcgcat gtggccttcg
    16681 atgcgcgtaa tcgggtcgac caccaggcgg cgtccggcat tattgatggt gtatccctga
    16741 gtttcgtact gagtgctcat gcctgtttat cctcattgcc tggctgatgt tcggtttctg
    16801 taggttgctg gttatgacgt ctgcgctggt caacggcgct ggcgactgcg tgcacaccaa
    16861 cagccgctgc caccacacca agcgcggtta aaccgacggt atcggcggtg gaatgagtac
    16921 ccatttgcgg aatatcgacc acgcggctgt agaacgaacc gcaatcccag aaaccatttt
    16981 ccgcacagcc caggcagccg tgaccagact ggattgggaa agaaacgcca tcattccagc
    17041 gtgtggagga acaggcgtta taggtggtag gccctttgca gcccattttg tacaggcagt
    17101 aacctttgcg ggcagcgtca tcatcccaac tctggacgaa ctctccggcg tcgaagtggg
    17161 cgcggcgata gcatttatcg tggattcgct gaccatagaa catcagcgga cggcccattc
    17221 tgtcgacatc tggcaagcga tcaaaggtca ccatgtaagt aatgatggcg ctcatcacat
    17281 ccgggatcgg cgggcagcca ggtactttga taatgggttt gtcggtgatg actttgtcga
    17341 taggcgttgc ctgcgtcgga ttgggccacg cggcctgcac gcagccccag gacgcgcagg
    17401 ttccccaggc gataatcgcg ctggctccgg cagcggcacg tttgagtttc tcaataaacg
    17461 gtcgaccgct gctgatacag aacatcccct gctcgcccag cggcggatta ccttctactg
    17521 cgaggatata tttgccattg tattgcgtga tgatgtcttc gaagacttct tccgcctggg
    17581 ttccggcggc agccatcaaa gtatcgtcat aatcgaggga aatcagggaa aggatgacgt
    17641 ccttcgccag tgggtgagcg gagcggataa aagattcggt acagcaggtg cattccagac
    17701 cgtggatcca taccaccgga atgcggggtt tgttctccag cgcccaggca atctttggtg
    17761 ccattcccgc gcctaatccc agcgacgtgg cagccagact acaatatttg agaaagctgc
    17821 gccgggtaac gccctgacgc cgcatggcct ggtaaaatgt ttcctcgtta ttcatatcgc
    17881 acgtctcttc tccttgcgac accggcagga caccgggcgc ttatggtcgg caagcgacgt
    17941 ccctgtagca aggcaaacca tcgcttcacg tgagatgctg aaaacgaaag ctcatccttc
    18001 tgcactggcg cacgtcgcca gaaagtattg ttaataaagc gtagtgaaa
//
LOCUS       DAZPLM010000072        17958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPLM010000072 DAZPLM010000000
VERSION     DAZPLM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.072"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHVC7_002086"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHVC7_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR2116135.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHVC7_002087"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHVC7_002087"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR2116136.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="ACHVC7_002088"
     CDS             4390..4641
                     /locus_tag="ACHVC7_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR2116137.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHVC7_002089"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHVC7_002089"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR2116138.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="ACHVC7_002090"
     CDS             5946..6704
                     /locus_tag="ACHVC7_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR2116139.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="ACHVC7_002091"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="ACHVC7_002091"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR2116140.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHVC7_002092"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHVC7_002092"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR2116141.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="ACHVC7_002093"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="ACHVC7_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="ACHVC7_002094"
     CDS             complement(9558..10178)
                     /locus_tag="ACHVC7_002094"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR2116142.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHVC7_002095"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHVC7_002095"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR2116143.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="ACHVC7_002096"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="ACHVC7_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR2116144.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="ACHVC7_002097"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="ACHVC7_002097"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR2116145.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="ACHVC7_002098"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="ACHVC7_002098"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR2116146.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHVC7_002099"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHVC7_002099"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR2116147.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="ACHVC7_002100"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="ACHVC7_002100"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR2116148.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="ACHVC7_002101"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="ACHVC7_002101"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR2116149.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4750 c         4488 g         4403 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccatc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcgat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtacgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgcggcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tcccccagcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgc
//
LOCUS       DAZPLM010000073        17770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPLM010000073 DAZPLM010000000
VERSION     DAZPLM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.073"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            55..733
                     /locus_tag="ACHVC7_002102"
                     /pseudo
     CDS             55..733
                     /locus_tag="ACHVC7_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            777..5627
                     /locus_tag="ACHVC7_002103"
     CDS             777..5627
                     /locus_tag="ACHVC7_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR2116150.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            5995..7650
                     /gene="lldP"
                     /locus_tag="ACHVC7_002104"
     CDS             5995..7650
                     /gene="lldP"
                     /locus_tag="ACHVC7_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR2116151.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7650..8426
                     /gene="lldR"
                     /locus_tag="ACHVC7_002105"
     CDS             7650..8426
                     /gene="lldR"
                     /locus_tag="ACHVC7_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR2116152.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8423..9613
                     /gene="lldD"
                     /locus_tag="ACHVC7_002106"
     CDS             8423..9613
                     /gene="lldD"
                     /locus_tag="ACHVC7_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR2116153.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9797..10270
                     /gene="trmL"
                     /locus_tag="ACHVC7_002107"
     CDS             9797..10270
                     /gene="trmL"
                     /locus_tag="ACHVC7_002107"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR2116154.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10323..11144)
                     /gene="cysE"
                     /locus_tag="ACHVC7_002108"
     CDS             complement(10323..11144)
                     /gene="cysE"
                     /locus_tag="ACHVC7_002108"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR2116155.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11224..12243)
                     /gene="gpsA"
                     /locus_tag="ACHVC7_002109"
     CDS             complement(11224..12243)
                     /gene="gpsA"
                     /locus_tag="ACHVC7_002109"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR2116156.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12243..12710)
                     /gene="secB"
                     /locus_tag="ACHVC7_002110"
     CDS             complement(12243..12710)
                     /gene="secB"
                     /locus_tag="ACHVC7_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR2116157.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12773..13024)
                     /gene="grxC"
                     /locus_tag="ACHVC7_002111"
     CDS             complement(12773..13024)
                     /gene="grxC"
                     /locus_tag="ACHVC7_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR2116158.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13166..13597)
                     /locus_tag="ACHVC7_002112"
     CDS             complement(13166..13597)
                     /locus_tag="ACHVC7_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR2116159.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13842..15386
                     /gene="gpmM"
                     /locus_tag="ACHVC7_002113"
                     /gene_synonym="pgmI"
     CDS             13842..15386
                     /gene="gpmM"
                     /locus_tag="ACHVC7_002113"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR2116160.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15420..16679
                     /gene="envC"
                     /locus_tag="ACHVC7_002114"
     CDS             15420..16679
                     /gene="envC"
                     /locus_tag="ACHVC7_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR2116161.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16683..17642
                     /locus_tag="ACHVC7_002115"
     CDS             16683..17642
                     /locus_tag="ACHVC7_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR2116162.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17629..>17770)
                     /locus_tag="ACHVC7_002116"
     CDS             complement(17629..>17770)
                     /locus_tag="ACHVC7_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR2116163.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4441 a         4606 c         4664 g         4059 t
ORIGIN      
        1 aatggacaac tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac
       61 ttgaagaaga tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt
      121 gactacattg aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacattaa
      181 aattgatcta cttgaaaaac aacaagcaca gctaaagagt caactggaaa ctattcaaaa
      241 acaacaaact ggcataataa gcagtacaaa gactttaact cacgtaatta aatccgttaa
      301 agatcaacaa aacactttta tttttaccga gtttaaccca gcaaaaacga agtatttcat
      361 cctgaacaat ggttcggtgg ctttagcggg tcgagtgtta tccattgacg ccacagagaa
      421 tggcagtgtt attcatattt cactggtcaa cttattaagt acacctatct caaatattgg
      481 ttttaatgcg acatggggtg gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca
      541 acaattactt ttcaacacat caatgaaatc cacattgaaa ttattaccag gtcaatggca
      601 agacattaat ttgaccctga agggtgtatc gcccaataac ctgggatatc tgaagttagc
      661 catcaacatg gaaaatattc agttcgacaa tcttccctct gctgaaaatc ggcagaaaag
      721 aagcaaaaaa taaatgcagt tagtacgctt ataacaagga atagcgagca tcattaatga
      781 acaaaatatt taaagttatc tggaaccctg cgacagggaa ttatactgtt accagcgaaa
      841 cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct gttaatttct gcgctggttg
      901 cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc cgggaatgac aacggccagg
      961 gtgttgatta cggtagtgga tcagctggcg acagctgggt tgctataggc aaaggggcga
     1021 aagcaaatac ttttatgaac accagtggtt ccagtactgc tgtgggttat gacgctatag
     1081 ctgaaggcca atatagctct gccatcgggt caaaaaccca tgcgattggt ggtgcatcaa
     1141 tggcctttgg ggttagtgca atatcagaag gcgatagaag tatagcactg ggtgcctctt
     1201 cgtattcatt gggccaatac tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat
     1261 tgtctattgc tatgggggac tcttccaaag cggaaggagc aaacgccatt gccctgggaa
     1321 atgccactaa agctactgag attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa
     1381 aagcgtattc aatggcgctg ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc
     1441 tggggcgtag cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg
     1501 ccagcgcagc gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg
     1561 ctattggcaa taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc
     1621 ttgccgataa agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg
     1681 caatcgccat aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg
     1741 gtagccagtc gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg
     1801 ataagagtct tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg
     1861 gcgcagattc cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac
     1921 gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg
     1981 gttcacagct ttatgccatt agcgactcgg tagcaaaaac gcttggagga ggggctgcag
     2041 tagatgttga tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa
     2101 ataacgtagg ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca
     2161 aaggcaaata cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg
     2221 ttgcggatgg cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag
     2281 ctaccaatga cgatgtcgaa gccaacaccg ccaatatcgc tactaatacc agcaacattg
     2341 ccacgaatac agcaagtatt gccaccaata ccaccaatat caccaacctg acggattccg
     2401 ttggtgacct tcaggctgat gccctgctct ggaacgaaac taaaaaggca ttcagtgcag
     2461 ctcacggcca ggataccacc agcaaaatca ccaacgttaa agatgccgac ctgacggctg
     2521 acagcactga tgctgttaac ggctctcagc tgaaaaccac caacgatgct gtggcgacga
     2581 ataccaccaa tatcgccaat aacacttcca atattgccac taacaccacc aacatctcta
     2641 atctgactga gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg
     2701 gtgtattcac ggcagctcat ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg
     2761 gcgacctgac gactggcagc accgatgccg ttaacggctc tcagctgaaa accaccaacg
     2821 atgccgtggc gacgaatacc accaatatcg ccactaacac caccaacatc tctaatctga
     2881 ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtct
     2941 tcactgcagc tcatggcaac aataccgcca gcaaaatcac caatatcctg gacggcacag
     3001 tcactgcaac cagttccgat gccattaacg gtagccagct ttatgactta agcagcaata
     3061 tcgccaccta cttcggcggc aatgcttctg tgaatactga cggtgtgttt accggtccaa
     3121 cctacaaaat cggtgaaaca aattattata acgtcggcga tgcactggct gcgattaact
     3181 cctcatttag cacgtctctc ggcgatgctc tgctttggga tgccaccgca ggtaaattca
     3241 gtgccaaaca cggtactaat ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa
     3301 tttcagactc cagttctgac gcagtaaacg gctcacaact ccacggcgtg agcagttatg
     3361 ttgttgatgc gctggggggt ggtgccgaag tcaatgcaga cggcaccatc actgcgccga
     3421 cgtacaccat tgctaatgct gattacgata atgtcggtga tgccctgaat gctatcgata
     3481 ccactcttga cgacgctctg ctctgggatg cggacgccgg tgaaaatggt gcatttagcg
     3541 ccgctcacgg aaaagataaa actgccagtg taatcactaa cgtcgctaac ggtgcaatct
     3601 ctgctgccag cagcgacgcg attaacggct cacaactcta taccaccaat aagtacatcg
     3661 ctgatgcgct gggtggtgac gcagaagtca acgctgacgg caccatcacc gcaccgactt
     3721 acaccattgc gaacgccgag tacaacaacg tcggtgacgc cctggatgcg cttgatgata
     3781 acgccctgct gtgggatgag actgccaatg gcggtgctgg agcctacaat gccagccatg
     3841 acggtaaagc cagcatcatc actaatgtcg ctaatggcag tattagtgag gacagtaccg
     3901 atgcagtgaa cggttctcag ttgaatgcga cgaatatgat gattgagcag aacacccaaa
     3961 ttatcaatca gctcgctggt aacaccgacg caacctatat cgaagaaaat ggcgctggta
     4021 tcaactacgt tcgtactaac gacaacgatt tagcctttaa cgatgcaagc gcctctggtg
     4081 tcggcgctac agctgtaggt tataacgctg tcgcgtctgg tgccagcagc gtagccattg
     4141 gtcagaacag cagcagcacc gttgataccg gtattgcgct gggtagcagc tccgtttcca
     4201 gccgtgtgat tgccaaaggt tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg
     4261 gttatgacac cactgacggc gaactgctag gtgcattgtc aattggtgat gacggtaaat
     4321 accgccaaat catcaacgta gctgatggtt cagaagccca tgacgccgtt acggttcgcc
     4381 agttgcagaa cgctattgga gcggtcgcca ctacgccaac caagtacttt cacgccaact
     4441 caacggcaga agactcactg gccgttggtg aagactcgct ggcaatgggt gcgaaaactg
     4501 tcgttaatgg taatgcaggt attggtatcg gtttgaacac tctggttcta gctgatgcga
     4561 tcaacggcat tgctatcggc agtaacgcac gcgcaaacca tgcaaacagt atcgcaatgg
     4621 gtaacggttc tcagaccacc cgtggtgcac agactggcta caccgcctac aacatggacg
     4681 caccgcagaa ctctgtaggt gagttttctg tcggtagtga agacggtcaa cgtcagatca
     4741 caaacgtcgc agctggttca gcggataccg atgcggttaa cgtgggtcag ttgaaagtca
     4801 ctgatgagcg cgtagcgcaa aatacccaga gcattactaa cctgaacaat caggtcacta
     4861 atctggatac tcgcgttact aatatcgaaa acggtattgg cgacattgtc accaccggta
     4921 gcaccaagta cttcaagacc aacaccgatg gcgtagatgc caacgcccag ggtaaagata
     4981 gcgttgctat tggttctggt tccattgctg ccgctgacaa cagcgtcgca ctgggtaccg
     5041 gttccgttgc agaggaagaa aatacaatct ctgtaggttc ttctactaac caacgccgta
     5101 tcaccaacgt agctgccggt gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat
     5161 cttctgaagc aggcggcgtg cgctacgaca ccaaagctga tggttctatc gactatagca
     5221 atatcaccct cggtggtggc aacggcagta cgactcgtat cagcaacgtc tccgctggcg
     5281 tcaacaacaa cgacgcggtg aactacgcgc agttgaagca aagcgtgcag gaaacgaagc
     5341 aatacaccga tcagcggatg gttgagatgg ataacaaact gtctaaaact gaaagcaagt
     5401 tgagcggtgg tatcgcttct gcaatggcaa tgaccggtct gccgcaggct tatacaccgg
     5461 gtgccagcat ggcttctatt ggtggcggta cttacaacgg tgaatcggca gttgctttag
     5521 gtgtatcgat ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata
     5581 gccagggtga atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaagt
     5641 tagtgtgact aagggcaaac gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa
     5701 ggcgccccgg gtcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc
     5761 agacatctcc ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc
     5821 acaattcccc aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt
     5881 cattgtcatt atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa
     5941 ggagatgagc aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat
     6001 ctctggcaac aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg
     6061 cttcccatcc tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc
     6121 gcctcatgga cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc
     6181 gctaacgcgc tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg
     6241 atcattattg cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc
     6301 attcgctcgt ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt
     6361 ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc
     6421 gccgcattgc tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt
     6481 gttaacaccg cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa
     6541 gtaacaggca tcgacagctt tgagattggc cagatggtgg ggcggcagct accgtttatg
     6601 accattatcg tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaggag
     6661 acgtggcctg cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctct
     6721 aacttcattg ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg
     6781 acgctgttcc tcaaacgctg gcagccagtg cgcgtattcc gttttggtga tttgggggcg
     6841 tcaaagattg atatgacgct ggcccacacc ggttacactg cgggtcaggt gttacgtgcc
     6901 tggacaccgt tcctgttcct gacagctacc gtaacactgt ggagcatccc gccgtttaaa
     6961 gccctgttcg catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac
     7021 ctcgataaac tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc
     7081 gtgtttaagt ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc
     7141 tcgattgtct ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg
     7201 aaagaactgg ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg
     7261 aactattccg gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc
     7321 accttcttct cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca
     7381 tctaacgccc tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat
     7441 ctgttgctgg ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa
     7501 tctatcgcta tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc
     7561 tttactgtca aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag
     7621 gcttatgtct taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg
     7681 aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt
     7741 tgcccgctga gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctgcgcgagg
     7801 cgctggcaaa actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta
     7861 ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga
     7921 tggccgatga tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca
     7981 gcaccgcatg gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt
     8041 gctttgaagc aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc
     8101 atctggcgat tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct
     8161 tcgatgtcct gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg
     8221 ttttttcaca actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg
     8281 ccgacggggc gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac
     8341 gattcgatga agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg
     8401 agcattcgag ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc
     8461 gcaacgcatt ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata
     8521 cacgctgcgc cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa
     8581 aaacatgtcc gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt
     8641 agcactggca ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc
     8701 caaagcggcg gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat
     8761 tgaagaagtc gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga
     8821 tcgcggcttt atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt
     8881 tttcaccgtg gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag
     8941 cggcccaaat gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg
     9001 ggatgtgggc ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa
     9061 accgaccgga ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc
     9121 atggaaagac cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat
     9181 cctcgatccg gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc
     9241 taaccacggt ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat
     9301 tgcagatgcg gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct
     9361 tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct
     9421 gtatgcgctg gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa
     9481 agagatgaaa gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga
     9541 ttcgctggtg caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg
     9601 gaatgcggca tagtcgtttg cgccccctca ccctcaggga gaggggaccg ttcggcgctg
     9661 tatgtactcc ctcactctga aacgacaccg cactcttttt ttctccctcg cccctccggg
     9721 gagggccggg gtgaggggaa aaggccgcac ggttcaccaa ttttctgcta tcctgccccc
     9781 gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac
     9841 tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccgat
     9901 gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac
     9961 cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct
    10021 gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga
    10081 ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc
    10141 cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga atctgtccaa
    10201 tgcggtgtcg gtagtggtgt atgaagcctg gcggcagctg ggatatccgg gagcgttgtt
    10261 gagagattag ttactgtatg ccggatagat gattacatcg catccggcac gatcacagga
    10321 cattagatcc catccccata ctcaaatgta tggttaatac cgttgaaatg ctggtccata
    10381 tccattgatg gcttatcgct gtctggttta ccgacaatac gagccggaac gccagcggcg
    10441 gttgtatgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcga
    10501 ccaacttcaa tattgccgag gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt
    10561 ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc
    10621 gatacgtcgt tttcaattac cgccgtttca cccacaacga tgcccgtggc gtggtcgagc
    10681 ataatgccac ggccaatttt tgctgccggg tgaatatcga cctggaacgt cacagaaacc
    10741 tggttttgca gaaagattgc caatgcgcga cgcccctgat tccacaacca gtgaccgatg
    10801 cgataggcct gcaaggcatg aaaacccttc aggtataaca acggggttga gtatttatcg
    10861 actgccgggt cgcgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc
    10921 gggtcagcgg cgtaggcttc ttccaccact tcacggatag caatagcagg cataattggc
    10981 gatgacagct tgttcgccag catgtagctc agtgcactac caaggttttc gtgcttgagt
    11041 agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt tctggcttcg
    11101 gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga
    11161 tgagataatg accggcccgt tctgcgcggg ccgggtcata gcggtaacaa aggttccctg
    11221 gggttagtgg ctgctgcgct cgtccttgcg tgcacgacct aataacgtca atgctgcctc
    11281 gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttctac
    11341 gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacctg
    11401 accaatcttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg
    11461 gcggttacgc gactggttgt cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc
    11521 cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacattt cagccagccc
    11581 acgggtgatc agtgccgtac gcgcattcgc accaaaaccg ataccgtcgg acatccccgc
    11641 gccaatggca atgacgtttt tcaccgcacc gccaagctgc acaccgatga aatccgggtt
    11701 gctgtaaacg cggaaacttt tgccacagtg cagcaattgt tggagatcat cggcaaaggt
    11761 ctggtcggta gaggccaacg aaatagccgt cggtaaacct gccgccagtt ctttcgcaaa
    11821 cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc
    11881 ctgtaacaga cgtccggttt ccgcttccag ccctttggtc gcccacacca gacgcgcatc
    11941 aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac
    12001 gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt
    12061 atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc
    12121 gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat
    12181 ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt
    12241 catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg
    12301 aacagcgcat cgaagttaat cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc
    12361 acgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcgcccagg
    12421 caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact
    12481 tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact
    12541 tcgtatacgt catctgccag ttgggaagat gccgtatcca gatcaagttt aacttctggt
    12601 tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat atccttggta
    12661 taaatacgtt ggatctggaa agtcatttca gtgttgtttt gttctgacat gtgtagaaaa
    12721 cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca
    12781 gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt
    12841 gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt
    12901 cacgcttggc ggcattgcca tcgatcggca actcctgaaa actcacgccc ttgctgctca
    12961 gcagtgcttt tgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg
    13021 ccataattta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat
    13081 ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc
    13141 tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggcaag ttttcgccag
    13201 cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg
    13261 cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt
    13321 tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac agattgatag
    13381 aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca accacagcgt
    13441 cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca
    13501 ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg atactcagta
    13561 tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta
    13621 gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac agccagcgcg
    13681 ttaactggaa tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct
    13741 tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    13801 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    13861 ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca
    13921 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    13981 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    14041 gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    14101 aaatcaaaga tcgcgctttc tttgctaatc cagtgctgac tggcgcggta gataaagcga
    14161 aaaacgcagg taaagcagta cacattatgg gtctgctctc cgcaggcggc gtacacagcc
    14221 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    14281 acctgcacgc attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga
    14341 aaaaatttga agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc
    14401 gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc
    14461 tgactctggc gcagggtgag tttcaggccg atactgccgt tgctggtttg caggcagctt
    14521 atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc
    14581 cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg
    14641 cgcgtgaaat cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag
    14701 tggttaacgt cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa actgcggttg
    14761 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    14821 ctcagttgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    14881 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    14941 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcca
    15001 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    15061 acaccggggt gatggaagcg gcggttaaag cggttgaagc actggatcac tgcgtggaag
    15121 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    15181 acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac accaacctgc
    15241 cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt
    15301 ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    15361 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    15421 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    15481 ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    15541 aatctattca ggccgatatc gccacaaaag agcgcgcggt acgccaaaag caacaacaac
    15601 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta
    15661 agctgcgcga aacgcaaaac acgctcaatc aactcaataa acagattgat gagatgaacg
    15721 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac
    15781 tggatgccgc attccgtcag ggcgagcata ccggtattca gctaattctc agcggtgaag
    15841 aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    15901 aaaccattgc ccagttgaag caaacgcgtg aagaagtcgc catgcagcgt gctgaactgg
    15961 aagagaaaca aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac
    16021 tgactcaggc tctgaacgag cgtaaaaaga cgctggcggg gctggaatct tccatccagc
    16081 aaggtcagca gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt aacagtattg
    16141 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gctcaggcag
    16201 ttcgcgacag gcagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg
    16261 aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc
    16321 cagttcgtgg ccctacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    16381 aaggaatggt tatcggtgcc tctgaaggta ctgaagttaa agcaattgcc gacggtcggg
    16441 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag cacggtaaag
    16501 gcgatatgag tctttacggc tataatcaaa gcgcgctggt gagcgttggt tcgcaggttc
    16561 gtgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggcaga ccttcactct
    16621 atttcgaaat tcgccgccag ggtcaggccg tcaatccaca gccgtggttg ggaagataag
    16681 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    16741 ctgtaattgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgaacaacg
    16801 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    16861 acgccagaga gatggcgacc aaagcgcata acagcggtca cgaagtgttg attcatctcc
    16921 cgatggcacc gttgagtaaa cagccgctgg agaaaaatac gctgcgcccg gagatgagca
    16981 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    17041 tcaataacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    17101 aggcgctgga gcgttataat ctttacttcc tcgacagcgt aaccattggt aatacccagg
    17161 cgatgcgcgc cgcgcaaggc actggcgtga aggtgatcaa acggaaggtg ttcctcgacg
    17221 attcacagaa cgaagcggac atccgtgtgc aattcaatcg cgcaattgac ctagcgcgtc
    17281 gcaacggatc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgttgcaac
    17341 agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    17401 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    17461 cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt tatgctaatc
    17521 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc
    17581 atcagtggca aggctggggc aaacagcctg aagcggcgaa gcttaacgct agcgtaaatt
    17641 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    17701 gaaagaccac agtaacgcct gataacccac ctttacgctg cgcacattgg taatcaggcg
    17761 cttatacata
//
LOCUS       DAZPLM010000074        17710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPLM010000074 DAZPLM010000000
VERSION     DAZPLM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.074"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHVC7_002117"
     CDS             <1..245
                     /locus_tag="ACHVC7_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR2116164.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHVC7_002118"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHVC7_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR2116165.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHVC7_002119"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHVC7_002119"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR2116166.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHVC7_002120"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHVC7_002120"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR2116167.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHVC7_002121"
     CDS             3423..4061
                     /locus_tag="ACHVC7_002121"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR2116168.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHVC7_002122"
     CDS             4066..4842
                     /locus_tag="ACHVC7_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR2116169.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHVC7_002123"
     CDS             4931..6295
                     /locus_tag="ACHVC7_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR2116170.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHVC7_002124"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHVC7_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR2116171.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHVC7_002125"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHVC7_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR2116172.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHVC7_002126"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHVC7_002126"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR2116173.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHVC7_002127"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHVC7_002127"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR2116174.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHVC7_002128"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHVC7_002128"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR2116175.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHVC7_002129"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHVC7_002129"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR2116176.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHVC7_002130"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHVC7_002130"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR2116177.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHVC7_002131"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHVC7_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR2116178.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHVC7_002132"
     CDS             complement(12844..13167)
                     /locus_tag="ACHVC7_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR2116179.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHVC7_002133"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHVC7_002133"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR2116180.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHVC7_002134"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHVC7_002134"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR2116181.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHVC7_002135"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHVC7_002135"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR2116182.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHVC7_002136"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHVC7_002136"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR2116183.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         4153 a         4749 c         4486 g         4322 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gcccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac
//
LOCUS       DAZPLM010000075        17447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPLM010000075 DAZPLM010000000
VERSION     DAZPLM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.075"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHVC7_002137"
     CDS             complement(<1..501)
                     /locus_tag="ACHVC7_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2116184.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHVC7_002138"
     tRNA            complement(687..771)
                     /locus_tag="ACHVC7_002138"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHVC7_002139"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHVC7_002139"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR2116185.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHVC7_002140"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHVC7_002140"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR2116186.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHVC7_002141"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHVC7_002141"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR2116187.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHVC7_002142"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHVC7_002142"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR2116188.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4654..5973
                     /gene="idnT"
                     /locus_tag="ACHVC7_002143"
     CDS             4654..5973
                     /gene="idnT"
                     /locus_tag="ACHVC7_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR2116189.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6040..7038
                     /gene="idnR"
                     /locus_tag="ACHVC7_002144"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6040..7038
                     /gene="idnR"
                     /locus_tag="ACHVC7_002144"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR2116190.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7116..8618
                     /locus_tag="ACHVC7_002145"
     CDS             7116..8618
                     /locus_tag="ACHVC7_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR2116191.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHVC7_002146"
     CDS             complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHVC7_002146"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR2116192.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHVC7_002147"
     CDS             complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHVC7_002147"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR2116193.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHVC7_002148"
     CDS             complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHVC7_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR2116194.1"
                     /translation="MSIIILATISTTQENK"
     gene            11186..12697
                     /gene="pepA"
                     /locus_tag="ACHVC7_002149"
     CDS             11186..12697
                     /gene="pepA"
                     /locus_tag="ACHVC7_002149"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR2116195.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12831..13274
                     /gene="holC"
                     /locus_tag="ACHVC7_002150"
     CDS             12831..13274
                     /gene="holC"
                     /locus_tag="ACHVC7_002150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR2116196.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13274..16129
                     /gene="valS"
                     /locus_tag="ACHVC7_002151"
     CDS             13274..16129
                     /gene="valS"
                     /locus_tag="ACHVC7_002151"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR2116197.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16185..17381)
                     /locus_tag="ACHVC7_002152"
     CDS             complement(16185..17381)
                     /locus_tag="ACHVC7_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR2116198.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4174 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacgtgcag cgcgaaagcg
     4621 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4681 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4741 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4801 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4861 atgttaggca agctgatttc tgatacgggc gcggcacaac gtatcgccac tacgctgatt
     4921 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4981 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgtg
     5041 gcatcatctg gattacccct gttgtatgtt ggcgtaccga tggtagcagc gctctctgta
     5101 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5161 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5221 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5281 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat ttttgctgca
     5341 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5401 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5461 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5521 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5581 ggcgcgttta agcaggtatt agtagatagt ggtgtcgggc agtatatttc acacttaatg
     5641 accggaacta cgctttcgcc gttattgatg tgctggactg tcgcggcgct gttgcgtatc
     5701 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgattatc
     5761 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ctggtgcggg cagcgtgatc
     5821 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5881 ggtgaaacgt tgcgtacctg gacagtgatg gaaaccctta tttctattat gggtttgctg
     5941 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     6001 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     6061 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6121 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6181 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6241 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6301 gtcacctctg tgcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6361 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat tctttcggaa
     6421 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6481 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggct
     6541 gcttttgaca tggtgtgtac catgctggat aagcgagtca ggcgcaaaat tctgtatctc
     6601 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6661 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6721 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6781 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6841 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6901 ctcgccagcg taattactcc ccgttttgat attggtcgga tggccgccca aatgttgctg
     6961 agcaagatta agaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     7021 cacggtaaca cgctttaggt tattttctct tcattacaca tattctcttt gccggacgtt
     7081 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7141 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     7201 ctggcgcaac ggggacgggt aaaactgtca cgttgcaaaa actggcagag tcattgtcgg
     7261 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc atcgcgcagg
     7321 caggaacggc gtcggaaaaa ctgctcgcaa gactgaaaaa tatcggcgtc aacgactggc
     7381 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7441 gggcgacggt ttcggatctg gggccgctgt tgctggcacg actgttgaat ctcaacgatg
     7501 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     7561 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     7621 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     7681 cgctggaaca gcaaggcgca gaacacttct ttggtgagcc gatgctggat atcaaagact
     7741 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7801 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttatatgaac
     7861 aattgcctga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaagcac
     7921 atctgctgtt taacgatgcg ccacaggtac tgctggataa gattgagcag gtgataaggc
     7981 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtca gatatcccgg
     8041 ataacgtgct agggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccta
     8101 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcgtttgata
     8161 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcga
     8221 aaggaagtcc ttcagtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     8281 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     8341 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     8401 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     8461 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     8521 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8581 ggatgttggg gagtttgctg ggggggagaa aaaggtaagt cagacgtgat ctgtgcccct
     8641 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     8701 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     8761 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     8821 ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     8881 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     8941 cagcgccatc aacatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     9001 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat agttgtgcaa
     9061 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     9121 gttggttttc caggtgccgc tcaccgtctg cgaaccggtg atctgtttcg gattggtcag
     9181 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     9241 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     9301 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     9361 ccataagcct tgctgagtgg agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     9421 acgcgccatc tgctcgccct gcgacgcgac ccattcacca atcgccatcg tcagcaagac
     9481 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     9541 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     9601 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     9661 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     9721 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     9781 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     9841 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     9901 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     9961 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
    10021 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
    10081 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgggcacga
    10141 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
    10201 accaccgcct ggtgaccaat gatcgcctga taatcctgaa agtccgtaat gcggaaatcg
    10261 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
    10321 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
    10381 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
    10441 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
    10501 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccatggtccc
    10561 gcccacatca cgttaaccgc cgcgacgatt gccgtgaaca ccgcaaggat cattgccgct
    10621 ttcaccagca ccgctttgct cagaccgcag gcatgcatta ccgtaatttc actttcggta
    10681 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
    10741 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
    10801 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
    10861 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
    10921 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
    10981 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
    11041 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
    11101 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
    11161 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
    11221 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
    11281 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
    11341 ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt ccgaatgtac tttccgagcg
    11401 aattctcctt attggttgcg gcaaagaacg tgagctggat gaacgtcagt acaagcaggt
    11461 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
    11521 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
    11581 tgcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
    11641 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
    11701 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
    11761 tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc agctggctga
    11821 cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga aagagctggg
    11881 gatgcactct tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga tgtcggtgat
    11941 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
    12001 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
    12061 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcag agctacaact
    12121 gcctattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
    12181 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
    12241 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
    12301 agcggtgatt gatgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
    12361 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
    12421 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
    12481 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
    12541 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg caggaaccgc
    12601 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
    12661 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
    12721 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
    12781 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
    12841 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
    12901 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    12961 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    13021 ttgtcccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    13081 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    13141 cagattttgc cactgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    13201 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    13261 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    13321 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    13381 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    13441 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctatggca
    13501 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    13561 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    13621 atggaaagcg gagtccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactctgt
    13681 cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt
    13741 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
    13801 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    13861 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    13921 tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg tggccgttaa
    13981 cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac cgctggttaa
    14041 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    14101 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    14161 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac
    14221 caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga
    14281 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcactcggcc tgctggaaga
    14341 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtag ttatcgaacc
    14401 aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cggttgaagc
    14461 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    14521 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    14581 tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa
    14641 ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    14701 gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ctgacgcact
    14761 gcgccagttc cacccaacca gcgtgatggt atccggcttc gacatcattt tcttctggat
    14821 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    14881 gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    14941 caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccgga
    15001 actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    15061 gcgcaccgag aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt
    15121 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    15181 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    15241 ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg cggaccgctg
    15301 gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    15361 cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    15421 tctggagctg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    15481 ccatacgctg gtgaccgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    15541 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    15601 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    15661 caccgaatgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaatat
    15721 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    15781 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    15841 gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct
    15901 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    15961 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    16021 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    16081 agcgaaagcg aaactgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    16141 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    16201 gaaggcataa ttccccgtga aatactggct aagaatcctt tatctggttg ttcttcttta
    16261 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    16321 atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga tatcaccacc
    16381 aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa acgaatacgc
    16441 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    16501 gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt acgttgcatt
    16561 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    16621 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagccaaa
    16681 aatgcgagaa tggcatattt tatacaatat gtagtatcta atttcacctt gaaacgatgt
    16741 ataccgaaac tggtattact ccagagaaaa ctcattacca gactatataa agtgccatta
    16801 aaaataccaa tgaaaacaat acaaactatt gtcatcagaa ccacagatat aataacgcta
    16861 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaagg
    16921 gccattaaaa tcggcaagaa aaaggtcacc caccagaacc ctttcataga gcaattaaaa
    16981 ctaaatctta cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc
    17041 ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaataag cggcatatct
    17101 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    17161 acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat attagcatag
    17221 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    17281 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac
    17341 gaatgggacg gaacatccat atcattaata acttgagcca taaaagttcc ctgaattaaa
    17401 aaatccattg atataattta tatatacaga cagtaaaatg ttctgcc
//
LOCUS       DAZPLM010000076        17391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPLM010000076 DAZPLM010000000
VERSION     DAZPLM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.076"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            47..1024
                     /gene="ybeQ"
                     /locus_tag="ACHVC7_002153"
     CDS             47..1024
                     /gene="ybeQ"
                     /locus_tag="ACHVC7_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR2116199.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1094..1576)
                     /locus_tag="ACHVC7_002154"
     CDS             complement(1094..1576)
                     /locus_tag="ACHVC7_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR2116200.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1811..4393
                     /gene="leuS"
                     /locus_tag="ACHVC7_002155"
     CDS             1811..4393
                     /gene="leuS"
                     /locus_tag="ACHVC7_002155"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR2116201.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4408..4989
                     /gene="lptE"
                     /locus_tag="ACHVC7_002156"
     CDS             4408..4989
                     /gene="lptE"
                     /locus_tag="ACHVC7_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR2116202.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4989..6020
                     /gene="holA"
                     /locus_tag="ACHVC7_002157"
     CDS             4989..6020
                     /gene="holA"
                     /locus_tag="ACHVC7_002157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR2116203.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            6022..6663
                     /gene="nadD"
                     /locus_tag="ACHVC7_002158"
     CDS             6022..6663
                     /gene="nadD"
                     /locus_tag="ACHVC7_002158"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR2116204.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6687..7298
                     /locus_tag="ACHVC7_002159"
                     /pseudo
     CDS             6687..7298
                     /locus_tag="ACHVC7_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7558..7875
                     /gene="rsfS"
                     /locus_tag="ACHVC7_002160"
     CDS             7558..7875
                     /gene="rsfS"
                     /locus_tag="ACHVC7_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR2116205.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7879..8346
                     /gene="rlmH"
                     /locus_tag="ACHVC7_002161"
     CDS             7879..8346
                     /gene="rlmH"
                     /locus_tag="ACHVC7_002161"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR2116206.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8377..10278
                     /gene="mrdA"
                     /locus_tag="ACHVC7_002162"
     CDS             8377..10278
                     /gene="mrdA"
                     /locus_tag="ACHVC7_002162"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR2116207.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10281..11393
                     /gene="mrdB"
                     /locus_tag="ACHVC7_002163"
                     /gene_synonym="rodA"
     CDS             10281..11393
                     /gene="mrdB"
                     /locus_tag="ACHVC7_002163"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR2116208.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11404..12492
                     /gene="rlpA"
                     /locus_tag="ACHVC7_002164"
     CDS             11404..12492
                     /gene="rlpA"
                     /locus_tag="ACHVC7_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR2116209.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12631..13842
                     /gene="dacA"
                     /locus_tag="ACHVC7_002165"
     CDS             12631..13842
                     /gene="dacA"
                     /locus_tag="ACHVC7_002165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR2116210.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13952..14215
                     /gene="ybeD"
                     /locus_tag="ACHVC7_002166"
     CDS             13952..14215
                     /gene="ybeD"
                     /locus_tag="ACHVC7_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR2116211.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14316..14957
                     /gene="lipB"
                     /locus_tag="ACHVC7_002167"
     CDS             14316..14957
                     /gene="lipB"
                     /locus_tag="ACHVC7_002167"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR2116212.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15217..16170
                     /locus_tag="ACHVC7_002168"
     CDS             15217..16170
                     /locus_tag="ACHVC7_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR2116213.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16379..17344
                     /gene="lipA"
                     /locus_tag="ACHVC7_002169"
     CDS             16379..17344
                     /gene="lipA"
                     /locus_tag="ACHVC7_002169"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR2116214.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4345 a         4391 c         4460 g         4195 t
ORIGIN      
        1 acataaaaat ataatatgtt ttatctacaa ccataaggaa atgataatgg tttttacgtc
       61 aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa aaggcgattg
      121 cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg actctccgga
      181 cgacgaaaag gccttttact ggctgaagct ggccgctgag caaggtcatt gtgaagcaca
      241 gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata atgagcaagc
      301 catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca acgcccttgg
      361 ctggacactg gatcgtggag aagcccccaa ttataaagaa gcggttgtct ggtatcagat
      421 agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt acagaaatgg
      481 caacggagtc gcaaaagact atgcgctggc atttttctgg tacaaacaag ctgcattaca
      541 aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa aaggcgttgc
      601 tcaaaacaag acactcgccg cattctggta tttgaaaagc gcacagcagg gtaatcggca
      661 cgcccagttt caaattgcat gggattataa cgctggcgaa ggggtggacc aggactataa
      721 gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa
      781 catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg ctgcctttga
      841 atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc tggccattat
      901 gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc tgtatcgtaa
      961 agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg aagatttact
     1021 gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt tattgctgtg
     1081 cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga ccacatttcg
     1141 ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga
     1201 ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga ttgaggtcct
     1261 ggaaaacttc gcgccattca agctgcgttt tatcggtgat atctgccagc tcctgccata
     1321 agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg ctctcttcat
     1381 agctcatggc gaactcttcc aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg
     1441 ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga
     1501 tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc gaccagttca cggtaatatt
     1561 gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat aatagacgtt
     1621 ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta tgaataatcc
     1681 gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg
     1741 ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc
     1801 actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca
     1861 ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg
     1921 cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac
     1981 catcggtgac gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat
     2041 cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc
     2101 ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt
     2161 tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca
     2221 gaaattcttc accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa
     2281 ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg
     2341 gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc
     2401 ttacgctgac gagctgctca acgatctgga taaactggat cactggccag acaccgttaa
     2461 aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa
     2521 cgactatgac aacatgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac
     2581 ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga
     2641 actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac
     2701 gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa cgggcgaaga
     2761 aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagttatggc
     2821 ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc tgaacatcaa
     2881 accagttatc ctggctgctg acggctctga gccagatctt tctcagcaag ccctgactga
     2941 aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt gaccatgaag cggccttcaa
     3001 cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct
     3061 gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct
     3121 ggaagacggt accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga
     3181 tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg caaaaactac
     3241 cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc
     3301 ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc
     3361 ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca
     3421 cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga
     3481 cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt
     3541 tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga
     3601 gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat
     3661 gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta
     3721 cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga
     3781 atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt
     3841 ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga
     3901 tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg atgatatcgg
     3961 ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc
     4021 gaaagcacca accgatggcg agcaggatcg cgctctgatg caggaagcgc tgctggccgt
     4081 tgtccgtatg cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa
     4141 aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga
     4201 agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct
     4261 ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa
     4321 atatcttgat ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct
     4381 ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacattgtt gttatctctg
     4441 gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg ataccacgca ggttccttcc
     4501 actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcagtg
     4561 cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac
     4621 gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc ggtattccgt
     4681 aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt gttgatcccc
     4741 ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cgttctttga taacccgcaa
     4801 atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta cgaccgtgct
     4861 gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa
     4921 gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc caccacgctg
     4981 ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc
     5041 gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg
     5101 ttcgtcaggt agctgcggca caaggattcg aagaacacca cactttttcc attgatccca
     5161 acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt gccagtcgac
     5221 aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc
     5281 tcacactcac cggacttctg catgacgacc tgctgttgat cgtccgcggt aataaattaa
     5341 gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga
     5401 cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc
     5461 tcaacttaga actggatgac gcggcaaatc aggtgctctg ctactgttat gaaggtaacc
     5521 tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaattga
     5581 cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct tttcattggg
     5641 ttgatgcttt gttgatggga aaaagtaagc gcgcattgca tattcttcag caactgcgtc
     5701 tggaaggcag cgaaccggtt attttgttgc gcacattaca acgtgaactg ttgttactgg
     5761 ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg
     5821 tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt
     5881 tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacggtc
     5941 agtcagtgtg ggcagagctg aaagggttat ctcttctgtt gtgccataaa cccctggcgg
     6001 acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat
     6061 ccggtgcact atggtcatct aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg
     6121 cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc
     6181 gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat
     6241 gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg
     6301 caggaacaag gaccggacgt gccgctggcg tttattattg gtcaggattc actgctgacc
     6361 tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg
     6421 cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat
     6481 ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa
     6541 acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg
     6601 tgtgaggatt tattgccgga accggtactg acttacatta accaacaagg cttgtatcgc
     6661 tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc
     6721 aagcgaatat cgatggtctt tacagcggtc atgcgcccac ccccctgacc gcgcgcggta
     6781 ttgagcaagc gcaaaatctg catacgctgc tacatggtgt ttcctttgat ctggttttat
     6841 gcagtgaact ggaacgggca cagcataccg cacgactggt tctcagtgac cgccagctcc
     6901 ccgtgcaaat catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc
     6961 atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc
     7021 atgcaatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta
     7081 tcgcaaggct tagtgaattt cagcactatc agaatatttt agtcgtcagc catcagggtg
     7141 tactgagtct gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg tggcattttc
     7201 gcgttgacta ggggtgttgg agcgccattg atatcaacca aaaattcgcg acgctacgcg
     7261 tcctcaatag ccgtgccatc ggggtcgaga atgcatgact tttctgtttt tttacgggta
     7321 agccgcaacg accattgaca gacccgggca ggctgatatt ctccgcagcc agactttttt
     7381 cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg
     7441 ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc
     7501 tggttcaggt atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg
     7561 cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac
     7621 atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat catctgtacg
     7681 ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca
     7741 gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat
     7801 ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa
     7861 aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac
     7921 tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag
     7981 ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg catactcgac
     8041 aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt
     8101 ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat
     8161 ggtcgcgacg tcagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg
     8221 gcggcggagc agagctggtc gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg
     8281 ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt
     8341 gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc
     8401 gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt
     8461 ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc
     8521 gactaccaga ctcgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc
     8581 ggcattatct acgaccgtaa tggtatccct ctggccctca accgcactat ctaccagata
     8641 gaaatgatgc cagagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg
     8701 gttgatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt
     8761 ttcacctcta ttccggtgaa aactaacctg accgaagtac aagtagctcg ctttgccgtc
     8821 aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta ctatccttac
     8881 ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa
     8941 cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat cggtaagctg
     9001 ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa
     9061 gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca agccggacac
     9121 gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt
     9181 agccgcgcag ctgtggttgt caccgatccg cgtacaggtg gggtgctggc gctggtttcc
     9241 acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga ttattccgcc
     9301 ttgttgaacg ccccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctccc
     9361 gcgtctacag ttaaacccta tgtggcggtt tcggcattga gcgccggggt gatcacgcgc
     9421 aatacgacgc tgtttgaccc aggctggtgg caactgccag gttcggaaaa acgttatcgt
     9481 gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg
     9541 gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg
     9601 ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg ttccggcaac
     9661 atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc
     9721 attccggttg gtatcggtca gggttactgg acagcgaccc caatccagat gagtaaggca
     9781 ctgatgatcc tgattaatga cggtatcgtg aaggttcctc atttgctgat gagcaccgcc
     9841 gaagacggca aacaggtgcc atgggtacag ccgcatgaac cgcccgtcgg cgatattcat
     9901 tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaacggt
     9961 acggcgcata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct
    10021 caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta
    10081 cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc
    10141 atgattctgg agaacggtgg tgcgggtccg gcggttggta cactgatgcg ccagatcctc
    10201 gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc agcggttgcc
    10261 gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc tgggataaag
    10321 tccatctcga tcccacaatg ctgctgatct tactggcatt gctggtttac agcgccctgg
    10381 ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaatc ggccaaatcg
    10441 cgatgggtct agtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct
    10501 gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg
    10561 ccatctctaa aggtgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg
    10621 aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc
    10681 cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg
    10741 tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac
    10801 tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt
    10861 tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc
    10921 tcctagaccc ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg
    10981 ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg
    11041 aatttctccc cgaacgccat accgacttta tcttcgcggt actggcggaa gagctgggat
    11101 tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga
    11161 tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta atgctgatat
    11221 tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg
    11281 ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg
    11341 ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc
    11401 gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca
    11461 agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg taacggcccc
    11521 atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag
    11581 gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttagccag
    11641 gcgggactgg cggcaatcta tgatgccgaa cccggcagta acctgacggc ctctggcgaa
    11701 gctttcgatc cgacacagct gacggcggcc catccaacgc ttccgatccc cagctacgcc
    11761 agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggccct
    11821 tacggcaacg accgcgttat ttcactttct cgcgcggcag ctgaccgtct taacacgtca
    11881 aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt
    11941 cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgccagc acctcccgat
    12001 ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat tcttccggtc
    12061 agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag tagcggtttc
    12121 ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc gacgcctgcg
    12181 ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca
    12241 ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc
    12301 gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt
    12361 cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc cagcaaagcc
    12421 gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattact
    12481 accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa
    12541 agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt
    12601 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    12661 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    12721 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    12781 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    12841 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    12901 aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac cggtaacccg
    12961 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    13021 ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    13081 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgcactgggc
    13141 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    13201 gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa tgaatactcg
    13261 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    13321 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    13381 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc
    13441 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    13501 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    13561 tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattcca
    13621 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    13681 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    13741 gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    13801 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    13861 tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt
    13921 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    13981 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    14041 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    14101 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    14161 tgaaacactg tatgaagaac taggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    14221 ttctccgtta cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    14281 ccccactttt actcattctc cacggagatg cagttttgta tcaggataaa attcttgtcc
    14341 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    14401 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    14461 aaggtcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg gtgatccaga
    14521 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtgttgc
    14581 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    14641 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg
    14701 tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    14761 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    14821 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    14881 ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    14941 aatatattac cgcttaattg catacctcaa tggcccaatt tacactgggt cattactcat
    15001 aatacctgct aatgctttac atctccttcg cgtttgtata ctttctttat cacttgcaga
    15061 aatgcggtgc tacgccttgc tccgtaactc accccattga ttaacctatt gaaatagttg
    15121 attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttaa
    15181 atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa
    15241 ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat
    15301 attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat
    15361 gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc
    15421 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
    15481 gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat
    15541 atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc ctcggtcgga
    15601 gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca gcttttactg
    15661 cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
    15721 atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
    15781 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact
    15841 atcgacaacg ctgcgcatgt actcctgtta ccggaagggc aaaatttcag cggtctgcgg
    15901 cagagagttc aagagatgtt tccggaccgg caaatcaatt tcaccagcta caacattttg
    15961 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
    16021 aacctgttta gtcgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
    16081 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg
    16141 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
    16201 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
    16261 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
    16321 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
    16381 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
    16441 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
    16501 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
    16561 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa
    16621 ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt gtccgttctg
    16681 tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga aactggcgca
    16741 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
    16801 gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg aaaaaagccc
    16861 gcaaatcaaa attgaaactc tggtgccgga tttccgtggt cgtatggatc gtgctctgga
    16921 tattctgact gcaacgccac cagatgtgtt caaccataac ctggaaaacg taccgcgtat
    16981 ttaccgtcag gtacggcctg gtgcagatta caactggtcg ctgaagctgc tggaacgctt
    17041 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggac tgggtgaaac
    17101 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac
    17161 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
    17221 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
    17281 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
    17341 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAZPLM010000077        17021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPLM010000077 DAZPLM010000000
VERSION     DAZPLM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.077"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHVC7_002170"
     CDS             <1..266
                     /locus_tag="ACHVC7_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR2116215.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHVC7_002171"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHVC7_002171"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR2116216.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHVC7_002172"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHVC7_002172"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR2116217.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHVC7_002173"
     CDS             complement(1570..3270)
                     /locus_tag="ACHVC7_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR2116218.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHVC7_002174"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHVC7_002174"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR2116219.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHVC7_002175"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHVC7_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHVC7_002176"
     CDS             4828..6600
                     /locus_tag="ACHVC7_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR2116220.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVSEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHVC7_002177"
     CDS             6593..8374
                     /locus_tag="ACHVC7_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR2116221.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHVC7_002178"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHVC7_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR2116222.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHVC7_002179"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHVC7_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR2116223.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHVC7_002180"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHVC7_002180"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR2116224.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHVC7_002181"
     CDS             11336..11908
                     /locus_tag="ACHVC7_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR2116225.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHVC7_002182"
     CDS             complement(11939..12250)
                     /locus_tag="ACHVC7_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR2116226.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHVC7_002183"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHVC7_002183"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHVC7_002184"
     CDS             12629..12982
                     /locus_tag="ACHVC7_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFR2116227.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     NVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHVC7_002185"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHVC7_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHVC7_002186"
     CDS             complement(14736..15206)
                     /locus_tag="ACHVC7_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR2116228.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHVC7_002187"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHVC7_002187"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR2116229.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHVC7_002188"
     CDS             complement(16044..16262)
                     /locus_tag="ACHVC7_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR2116230.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHVC7_002189"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHVC7_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR2116231.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4067 a         4122 c         4558 g         4274 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gtcggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc aatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat g
//
LOCUS       DAZPLM010000078        16854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPLM010000078 DAZPLM010000000
VERSION     DAZPLM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.078"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHVC7_002190"
     CDS             92..1132
                     /locus_tag="ACHVC7_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR2116232.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHVC7_002191"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHVC7_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR2116233.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHVC7_002192"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHVC7_002192"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR2116234.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHVC7_002193"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHVC7_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR2116235.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHVC7_002194"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHVC7_002194"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR2116236.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHVC7_002195"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHVC7_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR2116237.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHVC7_002196"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHVC7_002196"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR2116238.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHVC7_002197"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHVC7_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR2116239.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHVC7_002198"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHVC7_002198"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR2116240.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHVC7_002199"
     CDS             complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHVC7_002199"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR2116241.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12909..14090)
                     /locus_tag="ACHVC7_002200"
     CDS             complement(12909..14090)
                     /locus_tag="ACHVC7_002200"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR2116242.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14345..15574
                     /locus_tag="ACHVC7_002201"
     CDS             14345..15574
                     /locus_tag="ACHVC7_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2116243.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16034..16666
                     /locus_tag="ACHVC7_002202"
     CDS             16034..16666
                     /locus_tag="ACHVC7_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2116244.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4447 a         4422 c         3902 g         4083 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat
    11821 catcaaagac ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga
    11881 cgccggaatg gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt
    11941 gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga
    12001 aatagaaata aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt
    12061 tacctggagc cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga
    12121 caaaatattt gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag
    12181 atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt
    12241 aataaaactt tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt
    12301 taccaacata cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg
    12361 taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc
    12421 cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc
    12481 caacaataat tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct
    12541 tttcaaccaa aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt
    12601 ggatgccctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa
    12661 acgatagtga aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg
    12721 ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt
    12781 gtcgatcacg aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga
    12841 cttgccagcc atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa
    12901 atgcagagct actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa
    12961 agcgttgcca gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg
    13021 attcggcaac cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg
    13081 acccgacgct catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
    13141 tcattagcct cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg
    13201 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat
    13261 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
    13321 attaccgcag ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga
    13381 ctatcaaatg aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt
    13441 tcatcggtat caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca
    13501 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
    13561 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
    13621 atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg
    13681 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg
    13741 ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
    13801 agatgcagtg ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta
    13861 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
    13921 aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt
    13981 aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga
    14041 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
    14101 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
    14161 aaagtgattt ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac
    14221 gtaaggtttg caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata
    14281 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
    14341 gataatgtct aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac
    14401 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
    14461 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
    14521 ccagttcatc ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca
    14581 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
    14641 cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca
    14701 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa
    14761 tctcattttt ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc
    14821 attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct
    14881 attaacgacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
    14941 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
    15001 tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
    15061 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc
    15121 gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc
    15181 gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
    15241 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac
    15301 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
    15361 ggtatcgctg attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
    15421 tgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta
    15481 tctgatcccg gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg
    15541 tatcctgtgc gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg
    15601 ctacaatttt gaaccattcc atttttcata atattcattt gttattattc cgccctatca
    15661 cttctctcgt catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc
    15721 ttaaataaga ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa
    15781 aaacaatttg cgatatagat attctcatac catgagatag ttatctggac cacgttcgac
    15841 catgcaagat taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa
    15901 tgtcttaatc agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca
    15961 aaatcaagat gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag
    16021 ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc
    16081 aatgcattgc caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg
    16141 tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt
    16201 aacaaaaaac cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt
    16261 tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat
    16321 agttttagtc agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac
    16381 cgcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag
    16441 acaactattt attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga
    16501 attcaccata atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct
    16561 ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg
    16621 taaaataaac gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag
    16681 ccagaatcat actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc
    16741 agtaacccca agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca
    16801 ttacgaaata tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatt
//
LOCUS       DAZPLM010000079        16841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPLM010000079 DAZPLM010000000
VERSION     DAZPLM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.079"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            220..1272
                     /locus_tag="ACHVC7_002203"
     CDS             220..1272
                     /locus_tag="ACHVC7_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR2116245.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1588..2790)
                     /locus_tag="ACHVC7_002204"
     CDS             complement(1588..2790)
                     /locus_tag="ACHVC7_002204"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR2116246.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3022..4521
                     /gene="pitA"
                     /locus_tag="ACHVC7_002205"
     CDS             3022..4521
                     /gene="pitA"
                     /locus_tag="ACHVC7_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR2116247.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4765..5100)
                     /gene="uspB"
                     /locus_tag="ACHVC7_002206"
     CDS             complement(4765..5100)
                     /gene="uspB"
                     /locus_tag="ACHVC7_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR2116248.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5491..5925
                     /gene="uspA"
                     /locus_tag="ACHVC7_002207"
     CDS             5491..5925
                     /gene="uspA"
                     /locus_tag="ACHVC7_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR2116249.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6024..6077)
                     /locus_tag="ACHVC7_002208"
     CDS             complement(6024..6077)
                     /locus_tag="ACHVC7_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116250.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6243..7712
                     /gene="dtpB"
                     /locus_tag="ACHVC7_002209"
     CDS             6243..7712
                     /gene="dtpB"
                     /locus_tag="ACHVC7_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR2116251.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7761..8513)
                     /gene="rsmJ"
                     /locus_tag="ACHVC7_002210"
     CDS             complement(7761..8513)
                     /gene="rsmJ"
                     /locus_tag="ACHVC7_002210"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR2116252.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8521..10563)
                     /gene="prlC"
                     /locus_tag="ACHVC7_002211"
     CDS             complement(8521..10563)
                     /gene="prlC"
                     /locus_tag="ACHVC7_002211"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR2116253.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10766..11608
                     /gene="rlmJ"
                     /locus_tag="ACHVC7_002212"
     CDS             10766..11608
                     /gene="rlmJ"
                     /locus_tag="ACHVC7_002212"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR2116254.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11680..13032
                     /gene="gorA"
                     /locus_tag="ACHVC7_002213"
     CDS             11680..13032
                     /gene="gorA"
                     /locus_tag="ACHVC7_002213"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR2116255.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13086..13169)
                     /gene="dinQ"
                     /locus_tag="ACHVC7_002214"
     CDS             complement(13086..13169)
                     /gene="dinQ"
                     /locus_tag="ACHVC7_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR2116256.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13197..13370
                     /locus_tag="ACHVC7_002215"
     CDS             13197..13370
                     /locus_tag="ACHVC7_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116257.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13909..14262
                     /gene="arsR"
                     /locus_tag="ACHVC7_002216"
     CDS             13909..14262
                     /gene="arsR"
                     /locus_tag="ACHVC7_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR2116258.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14316..15605
                     /gene="arsB"
                     /locus_tag="ACHVC7_002217"
     CDS             14316..15605
                     /gene="arsB"
                     /locus_tag="ACHVC7_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR2116259.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15618..16043
                     /gene="arsC"
                     /locus_tag="ACHVC7_002218"
     CDS             15618..16043
                     /gene="arsC"
                     /locus_tag="ACHVC7_002218"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR2116260.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16177..16473)
                     /locus_tag="ACHVC7_002219"
     CDS             complement(16177..16473)
                     /locus_tag="ACHVC7_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR2116261.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16580..>16841
                     /locus_tag="ACHVC7_002220"
     CDS             16580..>16841
                     /locus_tag="ACHVC7_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2116262.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3877 a         4372 c         4275 g         4317 t
ORIGIN      
        1 agataattgt aaaaaaaaca acagaataag aattaatctt aggataaata tttatttatc
       61 atggcctttt aaccggtaaa aaatggcact cggcacagta aaccccgcta tagttatgct
      121 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
      181 cacatttcat tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtgg
      241 cttttcaaat taatcccttt gattatggga ttaatttgca tcgcgttagg tggctttgtg
      301 ctggaaagtt cagggcaaag cgagtatttc gtcgcgggtc atgtgctgat ttctctggcg
      361 gccatatgcc tggcattatt cactaccgca tttattatca tttcgcagct cacgcgcggc
      421 gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc aattatcacc
      481 atgatatggg gttgggcact gttagcaggc aatgatgtga tggcagacga gtttgtcgcc
      541 ggccatgtta ttttcggcgt tggtatgatt gccgcctgtg tatcgacggt ggcagcgtca
      601 tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga tggaacgccg
      661 gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt tttactcacc
      721 ctgctcggtt tcatttggtc tattacgctg ttacgtagtg ctgacataac tccgcattat
      781 gtcgcgggtc acgtattgct tgggttaacc gcaatctgtg cctgtctaat tggccttgtt
      841 gccacaattg tccatcaaac acgtaatacg ttttcaacta aagaacactg gctgtggtgt
      901 tattgggtta tttttctcgg ctcaatcacg gtactgcagg ggatatacgt cttagtcagt
      961 tccgatgcaa gcgcccgact ggctcccggc attattctta tttgcctcgg aatgatctgt
     1021 tacagcatat tctcaaaagt ctggctactg gcactggtct ggagacgtac ctgttcgtta
     1081 gccaacagaa taccgatgat acccgtcttc acctgcctgt tttgcctttt cctggcatca
     1141 tttcttgcgg aaatggcgca gaccgacatg ggatatttta ttccttcgcg agttctggtc
     1201 ggtttgggag cggtatgctt tacgttgttc tcaatcgttt caatattaga agcgggttct
     1261 gctaaaaaat aattgcaacg taccggataa aaccagcgtt gaccatttgc gtaacgctgg
     1321 tttttcttag gcatcatgaa ataacgcagc attaatgcat agtggttaag gataaaaaaa
     1381 caacaaagtt actgtctttt tcagcctgtt catattttat aaagatctgg ccactgggtg
     1441 agttttcaat cacctttcct tccaccttat attaagcctg gagggtttca gttcacgggc
     1501 tcattagaaa ataatcacaa gatagccact atcaaaatta gctcattttt aatgcggctc
     1561 taataatttt catctttagg taacaggtca ggacgacttc gctgcaatca aatcctgcgc
     1621 acaagcccac gcactcgacc acgcccactg gaagttatag ccccccagcc agccggtaac
     1681 gtccatcact tcgccgatga agtacagccc aggcactttg cgcgcttcca tcgtccgtga
     1741 agagagttcg ttggtgtcca cgccgccgag cgtcacttcg gcagtgcgat agccttcagt
     1801 gccgttgggt tgtacgcgcc agtcggtcaa tgtgctaatc agtgcctgtt ggtcacgcac
     1861 gttgagctgt tttagcgaaa cattcgggat ttgcccgagt tgctgtaagc gttcaaccaa
     1921 ccgcttcggt agatgaaccg ccagtgtgtt tttcaggctc tgattcggat gtgcgttacg
     1981 ctgctcattc aggaaggttt cgagatccac atccggtagc agattgatac tgacaaattc
     2041 acccggttgc cagtagctgg aaatctgcaa caccgccggt ccagacaagc cgcgatgggt
     2101 gaagagtaag ttctcacgga aaacgatgcc gttttcagcg gtaatcacgg aaggcaccgc
     2161 cacgcccgcc agcacctgta actcttcgag caacggttta tgcagagtga atggcaccag
     2221 acccgcacgg gtcggcagca cgttgaggcc aaattgttcg gcaatcttat aaccaaacgg
     2281 cgacgcgccc agccccggca ttgacagccc accggtcgcc atgaccagct tttcgcaacc
     2341 gacagtcatg ccgttcagat caagcgtgaa gcctgtttca tcctccgcca cactcagcac
     2401 ttcgctacgc aatctgaagg tcacattgcc cttctcgcac tcatccacca gcatgtcgac
     2461 aatctgctgc gcggagtcat cgcagaagag ttgccctaac gttttctcgt gccatgcgat
     2521 gccgtgttta ttgaccagat caatgaaatc ccactgggta aaacgtgcga gtgcagactt
     2581 acaaaaatgc ggattctggc tcagataagc gcctggttcg acataaaggt tggtaaagtt
     2641 gcagcgccca ccgccagaca taaggatttt gcgccctggt tttttaccat tatcgatcag
     2701 cagaacccgg cgtcctgcct gacctgccag cgcagaacag aacatacccg ccgcaccagc
     2761 gcctataata atggcatcaa acctttccac gttgcgctcc tcttagaaaa aacgggcgtg
     2821 aattgtaaag attcctcagt ggtcgcacca gcatcaatat tactaaaagg aagtatttgc
     2881 ctgaattata taagataatt attttttgag tgaaatccat acaggaggca aatcaaaaaa
     2941 agtctatatt tcactttgcc cgcgccgcga aagtcactga taatgcgccg cgttcatgtc
     3001 ctcaaaatgg cgtaacgtcc tatgctacat ttgtttgctg gcctggattt gcataccggg
     3061 ctgttattat tgcttgcact ggcttttgtg ctgttctacg aagccatcaa tggtttccat
     3121 gacacagcca acgccgtggc aaccgttatc tatacccgcg cgatgcgttc tcagctcgcc
     3181 gtggttatgg cggcggtgtt caactttttg ggtgttttgc tgggtggtct gagtgttgcc
     3241 tatgccattg tgcatatgct gccgacggat ctgctgctta atatgggatc gtctcatggc
     3301 cttgccatgg tgttctctat gttgctggcg gcgattatct ggaacctggg tacctggtac
     3361 tttggtttac ctgcatccag ctctcatacg ctgattggcg cgatcatcgg gattggttta
     3421 accaatgcgt tgatgaccgg gacgtcagtg gtggatgcac tcaatatccc gaaagtatta
     3481 agtattttcg gttctctgat cgtttcccct attgtcggcc tggtgtttgc tggcggtctg
     3541 attttcttgc tgcgtcgcta ctggagcggc accaagaaac gcgcccgtat ccacctgacc
     3601 ccagcggagc gtgaaaagaa agacggcaag aaaaagccgc cgttctggac acgtatcgcg
     3661 ctgatccttt ccgctatcgg cgtggcgttt tcgcacggcg cgaacgatgg tcagaaaggc
     3721 attggtctgg ttatgttggt attgattggc gtcgcgccag caggcttcgt ggtgaacatg
     3781 aatgccactg gctacgaaat cacccgtacc cgtgatgcca tcaacaacgt cgaagcttac
     3841 tttgagcagc atcctgcgct gctcaaacag gctaccggtg ctgatcagtt agtaccggct
     3901 ccggaagctg gcgcaacgca acctgcggag ttccactgcc atccgtcgaa taccattaac
     3961 gcgctcaacc gcctgaaagg tatgttgacc accgatgtgg aaagctacga caagctgtcg
     4021 cttgatcaac gtagccagat gcgccgcatt atgctgtgcg tttctgacac tatcgacaaa
     4081 gtggtgaaga tgcctggcgt gagtgctgac gatcagcgcc tgttgaagaa actgaagtcc
     4141 gacatgctta gcaccatcga gtatgcaccg gtgtggatca tcatggcggt cgcgctggcg
     4201 ttaggtatcg gtacgatgat tggctggcgc cgtgtggcaa cgactatcgg tgagaaaatc
     4261 ggtaagaaag gcatgaccta cgctcagggg atgtctgccc agatgacggc ggcagtgtct
     4321 atcggcctgg cgagttatac cgggatgccg gtttccacta ctcacgtact ctcctcttct
     4381 gtcgcgggga cgatggtggt agatggcggc ggcttacagc gtaaaaccgt aaccagtatt
     4441 ctgatggcct gggtgtttac ccttccggct gcggtactgc tttccggcgg gctgtactgg
     4501 ctctccttgc agttcctgta atcgtacgca ccaaaacgag cgggtcagct ggcccgcttc
     4561 agattgtgac atagtgcgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct
     4621 acaaaaacat gcaaattcaa tagattgcag agattatgta ggcctgataa gcgtagcgca
     4681 tcaggcaatt ttgcgtttgt aatcagtctc gagcgggtca gtttactggc ccgctttttt
     4741 tatgcgttat tattgctgaa gtgcttaatg ccaaatcatc aatgcaatca ggctgaccac
     4801 caccagacca cacaatgcgc tggtcagaat aaactgccga cgcacccgct cacagcggcg
     4861 aataaattca tcatcgtgat gatcgcgata acgttgggca tagatatacc aaacgagacg
     4921 cacctgtttg ttgggttggc catgtgaggt aaaaaagccc cctccatcaa catattgata
     4981 gagcaatgga tcgcagttac gcagtaccac taacaacgcg cgtagtgatg agaaatagcg
     5041 cgccatgtta acaatgcaaa cgacacataa agcccaaaat aatgcgacgg tgcttatcat
     5101 acctcctccc cggcgacctg cccgcggagt tccaccccgg ggctaccgct cccgatacgc
     5161 tgccaatcag ttaacaccag gtcctggaga aaccgctttt gtggtgacca acatccgagc
     5221 ggctctatag atagtgtagg agatcaggtt gttttttttc cagaaggtta accactatca
     5281 atatattcat gtcgaaaatt tgtttatcta acgggtaagc aaggcggatt gacggatcat
     5341 ccgggtcgct ataaggtaag gatggtctta acactgaatc tttacggctg ggttagcccc
     5401 gcgcacgtag ttcgcaggac gcgggtgacg taacggcaca agaaacgcta gctggccagt
     5461 catcgacaac tttatggaag gagtaacact atggcttata aacacattct catcgcggtc
     5521 gatctctccc cggaaagcaa agttctggta gagaaagcag tctctatggc tcgcccctac
     5581 aatgcgaaag tttctctgat ccacgtagat gtaaactact ctgacctata caccgggctt
     5641 attgatgtga atctgggtga tatgcagaaa cgcatctctg aagagacaca tcatgcgctg
     5701 accgagcttt ccactaatgc aggctaccca atcactgaaa ccctgagcgg cagcggcgac
     5761 ctgggccagg ttctggtcga tgcaatcaag aaatacgata tggatctggt ggtttgtggt
     5821 caccaccagg acttctggag caaactaatg tcttccgcac gtcagctgat caacaccgtt
     5881 cacgttgata tgctgattgt tccgctgcgc gacgaagaag aataatcatc cccccacgac
     5941 gtgttcctga acgcccgcat atgcgggcgt tttgcttttt ggcgcgcctt gttacctgat
     6001 gcagcgtaaa ccccttatct ggcctacgat ctgcgtacgc aatcaaaatc tcctgccaat
     6061 acaacattta acactatcat attttccatc attagtgtga tcagctggtt attttctgtt
     6121 gtaatagtgt attaatctat tcaccgcatc aatattaaga atctctgaca gatgtaaact
     6181 ttttcgcgcg ttatccctta cgcgttcata cttttcagga tggtattgga aggttaataa
     6241 atatgaatac aacaacaccc atggggatgc tgcagcaacc tcgcccattt ttcatgatct
     6301 tttttgtcga gttatgggag cgattcggct actacggcgt gcagggcgta ctggcggttt
     6361 tcttcgttaa acagcttgga ttctcgcaag agcaggcttt tgtcactttt ggtgcttttg
     6421 ctgcgctggt ctatggcctc atttccattg gcggctatgt cggcgaccac ctgctgggga
     6481 ccaaacgcac cattgttctc ggagcacttg tgctggcgat tggctacttc atgaccggca
     6541 tgtcgctact taagcctgac ctgattttca tcgccctggg gactatcgct gtcggtaacg
     6601 gcctgtttaa agctaaccca gccagcttgc tttcgaagtg ctatccgccg aaagatccgc
     6661 ggcttgatgg cgcattcacc ctgttctata tgtcgatcaa tatcggctcg ttgatagcgt
     6721 tatcgctggc ccctgtgatc gctgatagat acggttattc agtcacctac aacctgtgcg
     6781 gggcggggtt aattatcgca ttactggttt acatcgcctg tcgtggaatg gtgaaagaca
     6841 ttggttctga acccgacttc aagccgatga gcttcagcaa actgttgtac gtattacttg
     6901 gcagcgtggt gatgatcttc gtatgtgcat ggctgatgca caacgtagaa gtcgccaatc
     6961 tggtgctgat tgttctctcc atcgtcgtca ccatcatctt ctttcgtcag gcattcaagc
     7021 tggataaaac cgggcgcaat aaaatgtttg tcgcctttgt cctgatgctc gaagcggtgg
     7081 tgttttacat tctctacgcc cagatgccaa cgtcgctgaa cttctttgcc atcaacaacg
     7141 tgcatcatga aattctcggc ttttccataa acccggtcag cttccaggcg cttaacccgt
     7201 tctgggtggt actcgccagc ccaatactgg caggcattta cacgcatctg ggtaacaaag
     7261 gcaaagacct ctcgatgccg atgaaattta ctctcggcat gtttatgtgc tcactgggct
     7321 ttttgacggc ggcagctgcg ggaatgtggt ttgcggatgc acaagggctg acatcgccat
     7381 ggtttatcgt gctggtgtat ttattccaga gcctgggtga attgtttatt agcgcccttg
     7441 gcctggcgat gattgctgcc ctggtgccgc agcatttgat gggctttatt ctcgggatgt
     7501 ggttcctgac gcaggctgcc gcattcttgc tgggcggcta tgtggcaaca tttaccgcag
     7561 tgccggacaa cattaccgat ccgcttgaga cgttacccgt ctataccaac gtgtttggta
     7621 agattggcct ggttacgctg ggcgttgcag tagtgatgct gttgatggtg ccgtggctga
     7681 aacgcatgat tgcgacgccg gaaagccatt aattattctt gcagaaagca gggtagcgtt
     7741 atcgctaccc tgctttactt ttacacgggc gtacctgcat agatatcaaa gcgatgccct
     7801 ttagtcacaa ccgcgtttgg cgtggcgaca ttcgctagcg gtggcgcgta gtccgggcgc
     7861 tttactacca cgcgtttggt cgccagcaag cgtgcaggct ccagcaatcc atcggcatca
     7921 agatccggcc ccaccagcga ctgaaagaca cgcatctctt ttttcaccag cgcgcttttc
     7981 tgcttatgcg ggaacatcgg gtcgagatag accacctgcg ggcgtggggt aatatcggtt
     8041 agcgccgtca ggctggaggc gtgaattaac tgcaaacgct cctgcaacca gccgccgatt
     8101 tccgcatccg cataaccacg cgccaggccg tcgtcgagca gcgcggcaac aactggatta
     8161 cgctccagca tccgcacgcg gcaaccgact gaagccagca caaaggcatc gcgccccagt
     8221 cctgcagtgg catccaccac atccggcaaa taatcgcctt taatgcccac cgctttcgcc
     8281 accgcctcac cgcgaccacc gccgaatttg cgtcggtgcg ccatcgctcc gccaacaaaa
     8341 tcaacaaaga tgccgccaag ttttggctca tcacgcttgc gcaattccag atgttccggc
     8401 gttaacacca gcgccatcag gttgtcttca tcgtgctcca gcccccagcg ggccgccaga
     8461 acagataagg cgccgtctcc ggcgcctgtt tcatcaatta agcagatttt cactgaatga
     8521 tcagccctta atgccgtaat gctccagcat cgcatccagc tgcggttcac gaccacggaa
     8581 gcgtttgaac agatccatcg gctcttctga accgccacgg ctcagaatgt tgtcgaggaa
     8641 cgactgcccg gtttcacggt tgaaaatgcc ctcttcctca aagcgcgaga aagcatctgc
     8701 cgccagtacg tcagcccaca ggtagctgta gtaacctgcc gcataaccac cggcgaaaat
     8761 atggctgaaa gcgtgcggga aacggcccca ggacggagat ggcaccacgg caaccagttt
     8821 cttgatttct gccagagttt cgaggatttt tgccccctga tccgggcgga actcggcatg
     8881 aaggcggaaa tcaaacaggc cgaactccag ctgacgcaga ataaacagcg ccgcctggta
     8941 gttcttcgcc gccagcattt tatccagcaa ctctttcggc agcggttcgc cggtttcata
     9001 gtgaccagag ataaacgcca gcgcctccgg ctcccagcac cagttttcca taaactgact
     9061 cggcagttcg accgcatccc acggcacacc gctgataccg gaaacaccag cggtttcgat
     9121 gcgggtcagc atatggtgca ggccgtgacc gaactcgtgg aacagggtga tcacttcgtc
     9181 gtgagtgaac agcgccggtt taccatttac cgggcggttg aagttacaag tcaaatacgc
     9241 gaccggtttt tgcagagaac catcagcttt acgcatctgg cctacgcagt catccatcca
     9301 cgccccgccg cgcttgtttt cacgggcata cagatcgagg tagaagctgc cgcgcagttc
     9361 gttattttcg tcatacagtt cgaagaaacg tacatccgga tgccagacat caacctcttt
     9421 acgctcttta gcggtgatgc cgtaaatacg tttcaccact tcaaacaggc cgttaaccgc
     9481 tttgttttcc gggaagtacg gacgcagctg ctcgtcgctg atgctgtaga ggtgctgttt
     9541 ctgtttttcg ctgtagtaag cgatatccca cggctgcaac tcatcgacac caaattcggc
     9601 tttagcgaag gcacgcagtt gctccagctc tttttcgcct tgcggacgcg cgcgttttgc
     9661 cagatcggtt aagaaatcca gcacctgctg cgggttttct gccattttag tggcaaggga
     9721 tttaaaggcg tagttttcaa agcccagcag ttgcgccagt tcgtgacgca gcgcgaggat
     9781 ctcttccatc accttgctgt tatcccactt accggcgttc gggccttgat cggaggcgcg
     9841 ggtgctgtaa gcgcgataca tctcttcgcg cagagcctgg ttgtcgcagt aggtcattac
     9901 cggcaggtag cttgggatat ccagcgtcag caggtagcct tccagctctt tcgcttcggc
     9961 ctgggctttt gccgcagcca gcgcgctttc tggcatcccc gccagccccg cttcgtcggt
    10021 aacgagtttg gtccagccca tcgtcgcatc gaggacgttg ttgctgtact ggttgcccag
    10081 ttcagaaaga cgggtagcaa tttcgccgta acgctgctgt ttctctttcg gcagacctat
    10141 gccagagagt tcaaaatcac gcagggcgtt atcaaccgct ttcttctgcg ccgtgttcag
    10201 cgtggcgtaa tgatcgccat cgcgcaggtc gcggtacgct ttatacagcc cttcatgttg
    10261 ccctacccag gtgctgtatt ccgacagcag cggcagggtt tgctcgtagg cttcacgcag
    10321 ttccgggcta tttttcaccg agttcaggtg gctgaccggg gagaagatac gccccaacac
    10381 atcgtccact tccgccaacg gctggcagag attttcccag gtgtacggtg ccccttgcgc
    10441 tactacgcgc gccacatttt cgcggcaatc gttcagcgcc tgagtcacgg ctggaacgac
    10501 atgttccggg agaattttag aaaacggagg caattcaaag ggagtcagta acggattcgt
    10561 cattcgcgca gtcctggtta aagaggttaa ggaagcgctc aacaggcgct ttacataatg
    10621 tgtgtagcat ggggttaagt gtagtgaatt tcaatgagaa acgttacgct ttcgcggcgg
    10681 cggcctcttt tcggtatact gtcctgatac gcttttgtgc gccccgaata cgggccgatt
    10741 tttacttacc ggaacacctt tacccatgct cagttatcgc cacagctttc acgctggcaa
    10801 ccacgccgac gtccttaaac ataccgttca gagcctgatc atcgagtcgc tgaaagagaa
    10861 agataaaccg tttctctatc tcgataccca cgcaggggcc gggcgttatc agttaggcag
    10921 cgaacatgcc gagcgtaccg gcgaatatct cgaaggtatc gcccgtatct ggcagcagga
    10981 cgatttgccc gcagaactgg aggcgtacat caatgtggta aaacacttca accgtagcgg
    11041 tcagttgcgt tactaccccg ggtcgccgtt gattgctcgc cagctactgc gcgaacaaga
    11101 cagcctgcaa ctgaccgaac tgcacccgag cgattacccg ttgctgcgtt ctgaatttca
    11161 gaaagacagc cgtgcgcgcg tcgaaaaagc cgacggtttc cagcagctta aggccaaact
    11221 tccgccggtt tcccgccgtg gtttaatcct tatcgacccg ccgtatgaaa tgaaaaccga
    11281 ctatcaggcg gtggtcagcg ggatagcaga aggttacaaa cgtttcgcca ctggtactta
    11341 cgcactgtgg tatccggtgg tgctgcgtca gcaaattaag cgcatgatcc acgatctgga
    11401 agcgaccggt attcgcaaaa ttctgcaaat tgaactggcg gtactgccag acagcgatcg
    11461 ccgtggcatg accgcttccg gcatgattgt gattaacccg ccgtggaaac tggaacagca
    11521 gatgaataac gtgctgccgt ggctgcacag caaactggtt ccggcaggca ccgggcacgc
    11581 caccgtaagc tggatcgtgc cggagtaatt gcagccattg ctggcaccta ttacgtctcg
    11641 cgctacaatc gcggtaatca acgataagga cactctgtca tgactaaaca ctatgattac
    11701 atcgccatcg gcggcggcag cggcggtatc gcctccatca accgcgcggc tatgtacggc
    11761 cagaaatgcg cactgattga agccaaagag ctgggcggca cctgcgtgaa cgtcggctgc
    11821 gtgccgaaaa aagtgatgtg gcacgcggcg caaatccgtg aagcgatcca tatgtacggc
    11881 cctgattatg gtttcgatac caccatcaac aagttcaact gggaaacgtt gatcgccagc
    11941 cgtaccgcct atatcgaccg tattcatact tcctatgaaa acgtgctcgg taaaaataac
    12001 gttgatgtaa tcaaaggctt tgcccgcttc gttgatgcca aaacgctgga ggtaaacggc
    12061 gaaaccatca cggccgatca tattctgatc gccacaggcg gtcgtccgag ccacccggat
    12121 attccgggcg tggaatacgg tattgattct gatggcttct tcgcccttcc tgctttgcca
    12181 gagcgcgtgg cggttgttgg cgcgggttac atcgccgttg agctggcggg cgtgattaac
    12241 ggcctcggcg cgaaaacgca tctgtttgtg cgtaaacatg cgccgctgcg cagcttcgac
    12301 ccgatgattt ccgaaacgct ggtcgaagtg atgaacgccg aaggcccgca gctgcacacc
    12361 aacgccatcc cgaaagcggt agtgaaaaat gccgatggta gcctgacgct ggagctggaa
    12421 gatggtcgca gtgaaacggt ggattgcctg atttgggcga ttggtcgcga gcctgccaat
    12481 gacaacatca acctagaagc cgctggcgtt aaaactaacg aaaaaggcta tatcgtcgtc
    12541 gataaatatc aaaacaccaa tattgaaggt atttacgcgg tgggcgataa cacgggtgca
    12601 gtggaactga caccggtagc agttgcagcg ggtcgccgtc tctctgaacg cctgtttaat
    12661 aacaagccgg atgagcatct ggattacagc aacattccga ccgtggtctt cagccatccg
    12721 ccgattggta ctgttggttt aacggaaccg caggcgcgcg agcagtacgg cgacgatcag
    12781 gtgaaagtgt ataaatcctc tttcaccgcg atgtataccg ccgtcaccac tcaccgccag
    12841 ccgtgccgca tgaagctggt gtgcgttggg ccagaagaga agattgtcgg tattcacggc
    12901 attggctttg gtatggacga aatgttgcag ggcttcgcgg tggcgctgaa gatgggcgca
    12961 accaaaaaag acttcgacaa taccgtcgcc attcacccaa cggcggcaga agagttcgtg
    13021 accatgcgct aaatgttaaa gggctaagag tagtgtgctc ttagccctta attacgtttc
    13081 cgctatcagt tcagaagctg aagcagaaag cggatcagtt ccagcagcgc aattaacgct
    13141 ccaagaacga tgattgcttt atcaatcacc cgttttctcc atgcgatgga gtgagaatgc
    13201 atccgcttac tcatccactg cctgtcacgg cgcatgtctc attgttagat gacgactatc
    13261 tcactccggc cagagcatca gttaacggca ccacccgtac ttctgaccag gactttgaaa
    13321 gcgtttatgc gcattaccag agtgaaaatg cctcagagct aactggataa tcatacagta
    13381 cattcaggtt ataaaaccag catgtccttg caatagtttc agtatggtat tagcattgat
    13441 gcgttagatg atgactatct cactccagcc agagccacca actcagggct gaagagtaaa
    13501 aaaaccgacg caaagtcggt ttttttacat ccggattcgg acaaggctta atatgacgat
    13561 gacccagtga aagtatataa atcgtcattg cgatatatac cgaagtgccc cctccgccag
    13621 ctgaagaaat cgctaattct tgcaatgtta gccactggct aatagtattg aaccgttaga
    13681 tgtgggcatt ctcactccag ccagagccac caactcaggc tgaagagtaa aaaaccgacg
    13741 caaagtcggt ttttttacgt cctgattcaa accgcctttc aaatgaatag ccaactcaaa
    13801 attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata tgtttttgac
    13861 ttatccgctt cgaagagaga cactacctgc cacaatcagg agcgcaatat gtcatttctg
    13921 ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat cgttttactg
    13981 ctcagcgagc tgggagagtt atgcgtctgc gatctttgta ctgccctcga tcagtcgcag
    14041 cccaagatct cccgccacct ggcattgctt cgtgaaagcg ggctattgct ggaccgcaag
    14101 caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc ggcgaaaatt
    14161 attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg caacctggct
    14221 cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag ctaaacacat
    14281 atgaattttc agatgtgttt tatccgggag gcattatgtt actggcaggc gctatctttg
    14341 tcctgaccat cgtattggtt atctggcagc cgaaaggttt aggcatcggc tggagtgcaa
    14401 cgctcggcgc agtactggcg ttagttacgg gcgtggttca tccgggtgat attccggtgg
    14461 tgtggaatat cgtctggaac gcgacggctg cgtttatcgc cgtcattatc atcagcctgc
    14521 tgctggatga gtccggcttt tttgaatggg cggcgctgca cgtctcacgc tggggtaatg
    14581 gtcgtggtcg cttgctgttt acctggattg tcctgctcgg tgctgccgtt gccgccctgt
    14641 ttgccaatga tggcgcggcg cttattttga cgccgattgt catcgccatg ctgctggctt
    14701 taggattcag taaaggcacg acgctggcgt tcgtgatggc ggccggattc attgccgata
    14761 ccgccagcct gccgcttatt gtctccaacc tggtgaatat cgtttccgct gatttctttg
    14821 gcctcggctt tcgcgaatac gcctcggtga tggtgccggt ggatatcgcc gcgattgttg
    14881 ccacgctggt gatgctacat ctctattttc gcaaagatat tccgcagaac tacgatatgg
    14941 cgctgctgaa atctcccgca gaagcgatca aagatcctgc tacgttcaaa actggctggg
    15001 ttgttttact gcttctgctg gtgggatttt tcgtcctgga accgctcggc attccggtga
    15061 gcgccattgc agctgtgggc gcgctgatat tatttgtcgt cgctaaacgc ggtcatgcga
    15121 ttaatacggg taaagtcctg cgcggtgccc cctggcagat tgtcatcttc tcgctcggca
    15181 tgtatctggt ggtttatggc ctgcgcaatg ccggattaac ggaatatctt tctggcgtac
    15241 tcaacgtgct ggcggataac ggcctgtggg ccgcgacgct cggcaccgga ttcctcaccg
    15301 ccttcctctc ttctattatg aacaatatgc cgacggtact ggttggcgcg ttgtccattg
    15361 atggcagcac ggcatctggc gttatcaaag aagcgatggt ttatgccaat gtgattggct
    15421 gcgatttggg accgaaaatt accccaattg gtagcctggc tacgctactc tggctgcacg
    15481 tactttcgca gaagaatatg actatcagct ggggatatta cttccgtaca gggattatca
    15541 tgaccctgcc tgtgctgttt gtgacgctgg ctgcgctggc gctacgtctc tctttcactt
    15601 tgtaatgaga tactgatatg agcaacatta ccatttatca caacccggcc tgcggcacgt
    15661 cgcgtaatac gctggagatg atccgcaaca gcggcacaga accgaccatt atccattatc
    15721 tggaaactcc gccaacgcgc gatgaactgg tcaaactcat tgccgatatg gggatttccg
    15781 tacgcgcgct gctgcgtaaa aacgtcgaac cgtatgagga gctgggcctt gcggaagata
    15841 aatttactga cgatcggtta atcgacttta tgcttcagca cccgattctg attaatcgcc
    15901 cgattgtggt gacgccgctg ggaactcgcc tgtgccgccc ttcagaagtg gtgctggaaa
    15961 ttctgccaga tgcgcaaaaa ggcgcattct ccaaggaaga tggcgagaaa gtggttgatg
    16021 aagcgggtaa gcgcctgaaa taaagcggcg atatccactg ccgatagttg gtaaagtgcg
    16081 ctttgtttat gtcggatgcg acgctaacgc gtcttatccg acctacaaaa ccgtgcaaat
    16141 tcaatatatg gcatgaaata tgtagagcgt agcgcatcag gcaattttgc tatttacgca
    16201 gcactcctcg cgtaagaatt caattatcgc ttccagcatt gcgtattgcg aaacacacat
    16261 cagcgtccga ctctcacggt tttgtttgac cagtcctacc gataccagtg ctgatatatg
    16321 gtggctgagt gtggagccag gtatcccaag ctgcttttgc aactcaccga ccggcaaccc
    16381 ttgttccccg gctttgacaa gatgtttaaa tatgaacaaa cgggtcgggt gacctagctc
    16441 tttcagcgct ttagcgactt cttccaactg catatgacac ccctctgtct ggttgatatt
    16501 tcgataatac cagaaatatc ttgatctgaa tttcatttcg attattctgg aaatatagat
    16561 ttatccaatg aggttacgta tgagtagttg gcttgcgatg ctgcaagatg ccgcagagat
    16621 gtttgtgttt ctcgccgtcg agctttcgtt gctgtttata gtgattagtg ccggtgtcag
    16681 cctgataaga caaaaggtgc cagaccataa aatccagcag atgatggggg ccagaaaagg
    16741 gagaggctat ctcctggctg ctctgttggg agccgttacc ccgttctgta gttgctcgac
    16801 aatacccatg ttacgtggat tgttgtcagc gaaagccggg t
//
LOCUS       DAZPLM010000080        16594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPLM010000080 DAZPLM010000000
VERSION     DAZPLM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.080"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            160..312
                     /gene="mokC"
                     /locus_tag="ACHVC7_002221"
     CDS             160..312
                     /gene="mokC"
                     /locus_tag="ACHVC7_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR2116263.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(416..1546)
                     /gene="dnaJ"
                     /locus_tag="ACHVC7_002222"
     CDS             complement(416..1546)
                     /gene="dnaJ"
                     /locus_tag="ACHVC7_002222"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR2116264.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1635..3551)
                     /gene="dnaK"
                     /locus_tag="ACHVC7_002223"
     CDS             complement(1635..3551)
                     /gene="dnaK"
                     /locus_tag="ACHVC7_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR2116265.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3928..4332
                     /locus_tag="ACHVC7_002224"
     CDS             3928..4332
                     /locus_tag="ACHVC7_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR2116266.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4358..5071
                     /gene="msyB"
                     /locus_tag="ACHVC7_002225"
     CDS             4358..5071
                     /gene="msyB"
                     /locus_tag="ACHVC7_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR2116267.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5220..5786
                     /gene="satP"
                     /locus_tag="ACHVC7_002226"
     CDS             5220..5786
                     /gene="satP"
                     /locus_tag="ACHVC7_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR2116268.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5821..6408)
                     /gene="mog"
                     /locus_tag="ACHVC7_002227"
     CDS             complement(5821..6408)
                     /gene="mog"
                     /locus_tag="ACHVC7_002227"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR2116269.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6523..7476)
                     /gene="tal"
                     /locus_tag="ACHVC7_002228"
     CDS             complement(6523..7476)
                     /gene="tal"
                     /locus_tag="ACHVC7_002228"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR2116270.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7755..9184
                     /locus_tag="ACHVC7_002229"
                     /pseudo
     CDS             7755..9184
                     /locus_tag="ACHVC7_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9254..10030
                     /gene="yaaA"
                     /locus_tag="ACHVC7_002230"
     CDS             9254..10030
                     /gene="yaaA"
                     /locus_tag="ACHVC7_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR2116271.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10183..10479)
                     /locus_tag="ACHVC7_002231"
     CDS             complement(10183..10479)
                     /locus_tag="ACHVC7_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR2116272.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10693..11979)
                     /gene="thrC"
                     /locus_tag="ACHVC7_002232"
     CDS             complement(10693..11979)
                     /gene="thrC"
                     /locus_tag="ACHVC7_002232"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR2116273.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11980..12912)
                     /gene="thrB"
                     /locus_tag="ACHVC7_002233"
     CDS             complement(11980..12912)
                     /gene="thrB"
                     /locus_tag="ACHVC7_002233"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR2116274.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12914..15376)
                     /gene="thrA"
                     /locus_tag="ACHVC7_002234"
     CDS             complement(12914..15376)
                     /gene="thrA"
                     /locus_tag="ACHVC7_002234"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR2116275.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15403..15522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15458..15523)
                     /gene="thrL"
                     /locus_tag="ACHVC7_002235"
     CDS             complement(15458..15523)
                     /gene="thrL"
                     /locus_tag="ACHVC7_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR2116276.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15737..16423)
                     /locus_tag="ACHVC7_002236"
     CDS             complement(15737..16423)
                     /locus_tag="ACHVC7_002236"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR2116277.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3847 a         4409 c         4111 g         4227 t
ORIGIN      
        1 aatagcggcg ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa
       61 aagccccgag cgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga
      121 gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg
      181 attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa
      241 gacctctgtg aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac
      301 gaatccgagt aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat
      361 cttcaacgca ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc
      421 gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt
      481 tgtgctcacc ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct
      541 tctcgttcag gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc
      601 caccgcggac agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt
      661 cgccaggcac tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca
      721 gcgccgccat agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga
      781 aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct
      841 cgcccgcttc accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga
      901 ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg
      961 gatctttgat cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaga
     1021 atccctggcg catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg
     1081 tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga
     1141 tctctttggt cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat
     1201 cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt
     1261 caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt
     1321 gctcaaacgc agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa
     1381 cttcataagc ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac
     1441 ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg
     1501 ctgttttgga aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc
     1561 ccctagatga atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc
     1621 cgtttatagg gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg
     1681 tcatctttcg cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc
     1741 tgctgggcga tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg
     1801 atagcggctt tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata
     1861 gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg
     1921 tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct
     1981 tcggcgttag cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa
     2041 gaagccttga tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc
     2101 aagataccgt cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc
     2161 gggttgatac catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt
     2221 tcaccctgca gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc
     2281 tggctgtgct tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc
     2341 atggtttcga tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca
     2401 ccagtcagaa caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg
     2461 tctttacgcg gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga
     2521 gtctgaccac ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc
     2581 agtgcaactt tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt
     2641 ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac
     2701 ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct
     2761 tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg
     2821 aattcttcaa ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta
     2881 tcaccgttgg tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata
     2941 atagaaatat cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta
     3001 cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact
     3061 tccagaccag cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat
     3121 gccggtacgg tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc
     3181 ttcatttttt tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact
     3241 tcgacccatg cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca
     3301 cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg
     3361 ttttgcgggt tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc
     3421 tgggtatagg caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga
     3481 ggagtggtgc catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt
     3541 attttaccca tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta
     3601 ataggggcga aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact
     3661 gcggttgact actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt
     3721 ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat
     3781 tttattcata taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag
     3841 aaaagattac gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct
     3901 ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg
     3961 gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc
     4021 attgcaatgc cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt
     4081 gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt
     4141 tatttcaaag ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa
     4201 gggcaaacca ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca
     4261 aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc
     4321 ggcgacgact aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga
     4381 ttcagatctg gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt
     4441 gctcacccat aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact
     4501 gtttaaatcg atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg
     4561 agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt
     4621 gtatcgggcc attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat
     4681 gtctacgttt gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa
     4741 tatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct
     4801 ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt
     4861 gctttccagc caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca
     4921 tggtttgctg cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa
     4981 agcggtcagc ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct
     5041 gcgccggatg gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt
     5101 cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac
     5161 actactgtta ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta
     5221 tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca
     5281 ccattctgct taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca
     5341 tgggcatttt ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag
     5401 gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg
     5461 cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg
     5521 tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg
     5581 cacgcgttct gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg
     5641 gtaacattgc cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg
     5701 gtgccagtgc aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg
     5761 ttctgccgat tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt
     5821 ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc
     5881 cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc
     5941 gtgtaccaca acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt
     6001 cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga
     6061 aaggatcgca gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg
     6121 catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc
     6181 agttccgccc gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt
     6241 ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt
     6301 tagcgccgat gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct
     6361 ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc
     6421 gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca
     6481 gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat
     6541 cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc
     6601 tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg
     6661 acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc
     6721 cagctctttc agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat
     6781 ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta
     6841 gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt
     6901 cttatcagta ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa
     6961 cacgcctgct tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca
     7021 gttgatgcct tctttttcca gctgttctgc agcacggata ccctgccagg tagaagccag
     7081 tttgatcaga atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt
     7141 tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat
     7201 acggcccgga accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc
     7261 gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc
     7321 aatcaactta cgatattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt
     7381 ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt
     7441 ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct
     7501 tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg
     7561 ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc
     7621 gcccgccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta
     7681 gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa
     7741 taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg
     7801 taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc
     7861 agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag
     7921 gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca
     7981 acctggccgg cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt
     8041 gggttgccgc gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt
     8101 ataaagaacg tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg
     8161 ggctggggat gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca
     8221 taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt
     8281 tcccccgctg gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg
     8341 tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt
     8401 gctgaccagc atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc
     8461 tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt
     8521 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
     8581 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
     8641 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
     8701 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
     8761 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
     8821 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
     8881 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
     8941 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
     9001 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
     9061 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
     9121 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
     9181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
     9241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
     9301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
     9361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
     9421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
     9481 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     9541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     9601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     9661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     9721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     9781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     9841 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     9901 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     9961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
    10021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    10081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
    10141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
    10201 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
    10261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
    10321 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
    10381 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
    10441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
    10501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
    10561 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
    10621 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
    10681 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
    10741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
    10801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
    10861 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
    10921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
    10981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
    11041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
    11101 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
    11161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
    11221 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
    11281 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
    11341 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
    11401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
    11461 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
    11521 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
    11581 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
    11641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
    11701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
    11761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
    11821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
    11881 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
    11941 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
    12001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
    12061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
    12121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
    12181 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
    12241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
    12301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
    12361 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
    12421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
    12481 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
    12541 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
    12601 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
    12661 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
    12721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
    12781 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
    12841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
    12901 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
    12961 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
    13021 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
    13081 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
    13141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
    13201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
    13261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
    13321 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
    13381 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
    13441 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
    13501 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
    13561 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
    13621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
    13681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
    13741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
    13801 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
    13861 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
    13921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
    13981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
    14041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
    14101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
    14161 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
    14221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
    14281 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
    14341 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
    14401 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
    14461 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
    14521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
    14581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
    14641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
    14701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
    14761 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
    14821 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
    14881 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
    14941 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
    15001 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
    15061 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
    15121 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
    15181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
    15241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
    15301 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
    15361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
    15421 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
    15481 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
    15541 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
    15601 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
    15661 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
    15721 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
    15781 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
    15841 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
    15901 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
    15961 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
    16021 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
    16081 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
    16141 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
    16201 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
    16261 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
    16321 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
    16381 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
    16441 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
    16501 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
    16561 tgactgtact aacggtttaa ttgttaaaaa atgc
//
LOCUS       DAZPLM010000081        16572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPLM010000081 DAZPLM010000000
VERSION     DAZPLM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.081"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHVC7_002237"
     CDS             complement(<1..170)
                     /locus_tag="ACHVC7_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR2116278.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHVC7_002238"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHVC7_002238"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR2116279.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHVC7_002239"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHVC7_002239"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR2116280.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHVC7_002240"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHVC7_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR2116281.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHVC7_002241"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHVC7_002241"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR2116282.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHVC7_002242"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHVC7_002242"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR2116283.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHVC7_002243"
     CDS             4288..5151
                     /locus_tag="ACHVC7_002243"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR2116284.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHVC7_002244"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHVC7_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR2116285.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHVC7_002245"
     CDS             6489..7106
                     /locus_tag="ACHVC7_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR2116286.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHVC7_002246"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHVC7_002246"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR2116287.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLAHKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHVC7_002247"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHVC7_002247"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR2116288.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHVC7_002248"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHVC7_002248"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR2116289.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHVC7_002249"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHVC7_002249"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR2116290.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHVC7_002250"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHVC7_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR2116291.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHVC7_002251"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHVC7_002251"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR2116292.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHVC7_002252"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHVC7_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR2116293.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
BASE COUNT         4099 a         4501 c         4198 g         3774 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttatgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacttgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc ca
//
LOCUS       DAZPLM010000082        16402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPLM010000082 DAZPLM010000000
VERSION     DAZPLM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.082"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHVC7_002253"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHVC7_002253"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR2116294.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHVC7_002254"
     CDS             complement(2380..2820)
                     /locus_tag="ACHVC7_002254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR2116295.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHVC7_002255"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHVC7_002255"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR2116296.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHVC7_002256"
     CDS             3538..4236
                     /locus_tag="ACHVC7_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR2116297.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHVC7_002257"
     CDS             4465..5673
                     /locus_tag="ACHVC7_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2116298.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHVC7_002258"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHVC7_002258"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR2116299.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHVC7_002259"
     tRNA            7780..7856
                     /locus_tag="ACHVC7_002259"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHVC7_002260"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHVC7_002260"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHVC7_002261"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHVC7_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR2116300.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHVC7_002262"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHVC7_002262"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR2116301.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHVC7_002263"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHVC7_002263"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR2116302.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHVC7_002264"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHVC7_002264"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR2116303.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14612
                     /gene="dppF"
                     /locus_tag="ACHVC7_002265"
                     /pseudo
     CDS             13606..14612
                     /gene="dppF"
                     /locus_tag="ACHVC7_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
     gene            complement(14642..15913)
                     /locus_tag="ACHVC7_002266"
     CDS             complement(14642..15913)
                     /locus_tag="ACHVC7_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR2116304.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4096 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccccggac gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa
    14461 gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    14521 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta gcttgttttg
    14581 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    14641 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtac agcagaatca
    14701 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    14761 gaatgaggca cgacacaata ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    14821 atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag atcgccagag
    14881 tcagcacgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    14941 tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    15001 agatattaag caccgtagag gtgatatgaa tgatatgccc agggatcacc tgcgcggcca
    15061 gcgccagtgc tgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    15121 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    15181 gggtacgcag cgcgagccgg gttgccagta ctttatccgc ttcccgttta cgataggcaa
    15241 tattcattgg gttaagtacc tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa
    15301 gcaagacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    15361 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    15421 gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc agtacggcga
    15481 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    15541 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    15601 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    15661 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    15721 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    15781 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    15841 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    15901 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    15961 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    16021 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    16081 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    16141 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    16201 ggcaagccga aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    16261 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gtacctgaca acataacggg
    16321 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    16381 gcggctatat gacgctcgca ga
//
LOCUS       DAZPLM010000083        16199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPLM010000083 DAZPLM010000000
VERSION     DAZPLM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.083"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHVC7_002267"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHVC7_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR2116305.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHVC7_002268"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHVC7_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR2116306.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHVC7_002269"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHVC7_002269"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR2116307.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHVC7_002270"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHVC7_002270"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR2116308.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHVC7_002271"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHVC7_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHVC7_002272"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHVC7_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR2116309.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHVC7_002273"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHVC7_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR2116310.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHVC7_002274"
     CDS             7164..7604
                     /locus_tag="ACHVC7_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR2116311.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHVC7_002275"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHVC7_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR2116312.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHVC7_002276"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHVC7_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR2116313.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHVC7_002277"
     CDS             8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHVC7_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR2116314.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHVC7_002278"
     CDS             10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHVC7_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR2116315.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHVC7_002279"
     CDS             11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHVC7_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR2116316.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHVC7_002280"
     CDS             12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHVC7_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR2116317.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13149..13784
                     /gene="nth"
                     /locus_tag="ACHVC7_002281"
     CDS             13149..13784
                     /gene="nth"
                     /locus_tag="ACHVC7_002281"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR2116318.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHVC7_002282"
     CDS             14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHVC7_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR2116319.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16003..16122
                     /locus_tag="ACHVC7_002283"
                     /pseudo
     CDS             16003..16122
                     /locus_tag="ACHVC7_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
BASE COUNT         3756 a         4122 c         4137 g         4184 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca gcaacaggct
    10561 aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg ccgtcgaagc cgctattgcc
    10621 cgtgccaaag cgcgcaagct ggaacagcaa cagactaacg cagaaccaga agaacaggtt
    10681 gatccgcgca aagcggcagt tgccgcggct attgcccgcg ctcaggccaa aaaagccgcc
    10741 cagcagaagg ttgtaaacga ggactaaatg gtattcagaa tagctagctc cccttatacc
    10801 cataaccagc gccagacatc gcgcattatg ctgttggtgt tgctcgcagc cgtgccagga
    10861 atcgcagcgc aactgtggtt ttttggttgg ggtactctcg ttcagatcct gttggcatcg
    10921 gttagtactc tgttagccga agctctcgta ctcaaactac gcaagcagtc ggtagccgca
    10981 acgttgaaag ataactcagc attgctgaca ggcttattgc tggcggtaag tattcccccc
    11041 ctcgcgccat ggtggatggt cgtgctgggt acggtgtttg cggtgatcat cgctaaacag
    11101 ttgtatggcg gtctggggca aaacccgttt aatccggcaa tgattggtta tgtggtctta
    11161 ctgatctcct tcccggtgca gatgaccagc tggttaccgc cacatgaaat tgcggtcaac
    11221 atccctggtt ttatcgacgc catacaggtt attttcagcg ggcataccgc cagtggtggt
    11281 gatatgaaca cactacgctt aggtattgat ggcattagtc aggcgacacc gctggataca
    11341 tttaaaacct ctgtccgtgc cggtcattcg gttgaacaga ttatgcaata tccgatctac
    11401 agcggtattc tggcgggcgc tggttggcaa tgggtaaatc tcgcctggct ggctggcggc
    11461 ctgtggttgc tatggcagaa agcgattcgc tggcatattc ccctcagctt cttagtaacg
    11521 ctggcgttat gcgcaacgtt gggctggttg ttctcaccag aaacactggc agcaccgcaa
    11581 attcatctgc tgtctggtgc gaccatgctc ggcgcattct ttattttgac tgacccggtt
    11641 accgcttcta cgaccaatcg tggtcgtctt attttcggcg cgcttgcggg cttattagtc
    11701 tggttgatcc gcagtttcgg cggctatcct gacggcgtgg cttttgccgt cctgctggcg
    11761 aacatcacgg ttcctctgat cgattactac acgcgtccgc gcgtctacgg ccatcgcaaa
    11821 gggtaaacca tgctgaaaac tatccgaaaa cacggcatta cgttagcgct atttgcagcg
    11881 ggttcaacag ggttaactgc ggccatcaac cagatgacca aaacgacgat tgctgaacag
    11941 gccagtctgc aacaaaaggc attatttgat caggtgctgc cagccgaacg ctataacaat
    12001 acgctggcac agagttgcta tctggtaact gcgccagagt taggtaaagg tgagcatcgg
    12061 gtttacatcg ccaaacagga tgacaaaccg gtagccgccg ttctggaagc aaccgcgcca
    12121 gatggctatt ccggtgcgat tcagctgctg gtgggagccg attttaacgg cacggtactt
    12181 ggcacgcgcg tgacagagca ccacgaaacg ccagggcttg gcgataaaat cgaactgcgc
    12241 ctttctgact ggatcaccca ttttgcgggt aaaaaaatca gtggtgcgga tgatgcgcac
    12301 tgggcggtga agaaagatgg tggtgatttc gaccagttca ccggcgcgac gattactccc
    12361 cgcgcggtgg ttaatgcggt aaaacgcgcc ggattgtacg ctcagacgtt accggcacaa
    12421 ctttctcaac ttcctgcctg tggagaataa agcgtgagcg aaattaaaga cgttattgtt
    12481 caggggttgt ggaaaaacaa ctctgcgctg gtccagttgc tcggcctttg tcctctgttg
    12541 gcggtcacgt ccactgccac taacgctctg ggtttaggac ttgcgactac gctggtactg
    12601 acgctgacca acctgaccat ttcgacgctg cgtcactgga cgccagccga gatccgcatt
    12661 cccatttacg tgatgatcat cgcctcggtg gtcagcgctg tacagatgct gatcaacgcc
    12721 tacgcctttg gcctgtatca atcattaggg atttttattc cgctgattgt cactaactgt
    12781 atcgttgtgg gccgcgctga agccttcgcc gccaaaaaag gtccggcgct ttcggcactg
    12841 gacggctttt caattggtat gggcgcaacc tgcgccatgt tcgtgctggg ttcactacgc
    12901 gaaattatcg gcaatggcac attgtttgac ggtgcagatg cgctgttagg tagctgggca
    12961 aaagtattac gcgtggagat tttccacacc gactcccctt tcctgctggc gatgctgcca
    13021 ccaggtgcat ttattggcct gggactgatg ctggcaggaa aatacctgat tgatgaaaga
    13081 atgaaaaagc gccgtactga agcagctgcc gaacgtgcat tgccaaacgg tgaaacaggg
    13141 aatgtctgat gaacaaagca aaacgcctgg agatcctcac tcgcctgcgt gagaacaatc
    13201 ctcatcccac caccgagctt aatttcagtt cgccttttga attgctgatt gccgtactgc
    13261 tttccgctca ggcgaccgat gtcagtgtta ataaggcgac ggcgaaactt tacccggtgg
    13321 cgaatacgcc tgcagcgatg cttgaactgg gcgttgaagg ggttaaaacc tatatcaaaa
    13381 cgattgggct ttataacagc aaagcagaaa atatcatcaa aacctgccgt atcttactgg
    13441 agcagcataa tggcgaggtt ccggaagatc gtgctgcgct tgaagccctg cccggcgtag
    13501 gtcgtaaaac agccaacgtc gtattaaaca ctgcattcgg ctggccgact attgctgtcg
    13561 acacgcacat tttccgcgtt tgtaatcgta ctcaatttgc gccggggaaa aacgtcgaac
    13621 aggtagaaga aaagctactg aaagtggttc cggcagagtt taaagtcgac tgccaccatt
    13681 ggttgatcct gcacgggcgt tatacctgca ttgcccgcaa gccccgctgt ggctcttgta
    13741 ttattgaaga tctttgtgaa tacaaagaga aagttgacat ctgaagaaaa ggggtaacac
    13801 cgattacccc attgataacc tttctttatc ctcttttaaa acatcttttt aacgtgaatg
    13861 atgccattgc ttagcgttat catcaggtaa tccgtttgcg gataaccaaa aatgcaggtt
    13921 aattgttttt ttaatagcga aattttctat tcattcgtga tcaagatcac gatgctgtcg
    13981 caatgtaaaa acattgcaag aatcaggtta aaataattta aatttacaca cgcaacaaat
    14041 attgacctac aaaacattac actggctatt tttcagaaac tggactatct cactagtaaa
    14101 aacgccaaat agcagaacat atcgccgtta agccatcacc ccccctctca gtgcagtgaa
    14161 aaaatctgcc attacgtttt ttgaaaaatt taacgctgga taacatttcc cggaatggtt
    14221 gaattccccg cctcagttat atgtaacaga ttattacaaa ggacttgtct gaaagtgcaa
    14281 gatagtgaac attacctgcc gtttcccctc ccactataac aattgcgcgt atgtttttta
    14341 gacataacgc gagaaagccc ccccgttaat atgggatgta aaaaaagagg taaaagtgtc
    14401 cactgcaaac caaaaaccaa ctgaaagcgt cagtttgaac gctttcaaac aaccgaaggc
    14461 gttctatctc atcttctcga ttgagttatg ggaacgtttt ggttattacg gcctacaagg
    14521 aattatggct gtttacctgg ttaaacaact gggtatgtct gaagcggatt caatcaccct
    14581 tttctcttcc tttagtgccc tggtttatgg tctggtcgct atcggcggct ggttaggtga
    14641 caaggtactg ggtactaaac gcgtaattat gctcggcgct attgtgctgg cgattggtta
    14701 tgctctggtt gcctggtctg gtcacgacgc cggtatcgtt tatatgggta tggcggctat
    14761 tgcggtcggt aacggcctgt ttaaagctaa cccatcttct ctgctttcta catgctatga
    14821 gaaaaacgac ccgcgtctgg acggtgcatt caccatgtac tacatgtccg tcaacatcgg
    14881 ctctttcttc tctatgattg ctacgccgtg gctggccgcg aaatacggct ggagtgttgc
    14941 gtttgcgttg agcgttgtag gcctgctgat cactatcgtt aacttcgcct tctgccaacg
    15001 ctgggttaaa cagtacggtt caaaaccaga cttcgagcct atcaactacc gtaacctgct
    15061 gctgaccatt attggtgttg tggcactgat cgctatcgcc acctggctgc tgcacaatca
    15121 ggaagttgcg cgtatggcgc tgggcgttgt tgccttcggt atcgtggtta tcttcggtaa
    15181 agaagccttc gcgatgaaag gtgctgcgcg tcgtaaaatg atcgttgcct tcatcctgat
    15241 gctcgaagcc attatcttct tcgtgctgta cagccagatg ccaacgtcac tgaacttctt
    15301 tgcgattcgt aacgttgagc actccattct gggtctggcc gtagaacctg agcagtatca
    15361 ggcactgaac ccgttctgga tcatcatcgg tagtccgatt ctggccgcta tctataacaa
    15421 gatgggcgat accctgccga tgccaaccaa gtttgcaatc ggcatggtga tgtgttctgg
    15481 tgcgttcctg attctgccgc tgggtgcgaa attcgcgtct gacgctggta tcgtgtctgt
    15541 aagctggctg gtcgcaagct atggcctgca gagcatcggg gaactgatga tctctggtct
    15601 gggtctggca atggttgcac aactcgttcc gcagcgtctg atgggcttca ttatgggtag
    15661 ctggttcctg accactgccg gtgcaaacct gattggtggt tatgttgcgg gtatgatggc
    15721 tgtgccggat aacgttaccg acccgctgat gtcactggaa gtttatggtc gcgtattctt
    15781 gcagattggt gtcgctactg ccattattgc agtactgatg ctgctgaccg cgcctaaact
    15841 gcaccgtatg acgcaggatg acgctgcaga caaagcggcg aaagctgctg tagcgtaaaa
    15901 ttcagggaaa ctcttttaca agccgctaac ttttcgttag cggctttttt ttgttcagca
    15961 acgcactacc atactttaaa ccacagacaa aaggagttac cgatgaaatt gttctacaaa
    16021 ccgggtgcct gctctctcgc ttcccatatc accctgcgtg agagcggaaa ggattttacc
    16081 ctcgtcagtg tggatttaat gaaaaaacgt ctcgaaaacg gtgtaatgcc gatcagttaa
    16141 ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact tgtttccgg
//
LOCUS       DAZPLM010000084        16049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPLM010000084 DAZPLM010000000
VERSION     DAZPLM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.084"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(37..1860)
                     /gene="typA"
                     /locus_tag="ACHVC7_002284"
     CDS             complement(37..1860)
                     /gene="typA"
                     /locus_tag="ACHVC7_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR2116320.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2233..3642
                     /gene="glnA"
                     /locus_tag="ACHVC7_002285"
     CDS             2233..3642
                     /gene="glnA"
                     /locus_tag="ACHVC7_002285"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR2116321.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3816..4865
                     /gene="glnL"
                     /locus_tag="ACHVC7_002286"
     CDS             3816..4865
                     /gene="glnL"
                     /locus_tag="ACHVC7_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR2116322.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4877..6286
                     /gene="glnG"
                     /locus_tag="ACHVC7_002287"
     CDS             4877..6286
                     /gene="glnG"
                     /locus_tag="ACHVC7_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR2116323.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6398..6508
                     /locus_tag="ACHVC7_002288"
     CDS             6398..6508
                     /locus_tag="ACHVC7_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR2116324.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6698..8071)
                     /gene="hemN"
                     /locus_tag="ACHVC7_002289"
     CDS             complement(6698..8071)
                     /gene="hemN"
                     /locus_tag="ACHVC7_002289"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR2116325.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8260..8769)
                     /gene="yihI"
                     /locus_tag="ACHVC7_002290"
     CDS             complement(8260..8769)
                     /gene="yihI"
                     /locus_tag="ACHVC7_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR2116326.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8888..9054)
                     /locus_tag="ACHVC7_002291"
                     /pseudo
     CDS             complement(8888..9054)
                     /locus_tag="ACHVC7_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9351..9983
                     /gene="yihA"
                     /locus_tag="ACHVC7_002292"
     CDS             9351..9983
                     /gene="yihA"
                     /locus_tag="ACHVC7_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR2116327.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10365..13151)
                     /gene="polA"
                     /locus_tag="ACHVC7_002293"
     CDS             complement(10365..13151)
                     /gene="polA"
                     /locus_tag="ACHVC7_002293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR2116328.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13515..14447
                     /locus_tag="ACHVC7_002294"
     CDS             13515..14447
                     /locus_tag="ACHVC7_002294"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR2116329.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14488..15918)
                     /locus_tag="ACHVC7_002295"
     CDS             complement(14488..15918)
                     /locus_tag="ACHVC7_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR2116330.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4098 c         3942 g         4206 t
ORIGIN      
        1 cagcgatggc aggtttttta taactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt
       61 ggcgcgacga cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt
      121 cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat
      181 aggcggaacc agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt
      241 accggtcagg cagtttacag tcaggtcgtt agagcggctg tgaataccga taatctgacc
      301 ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cggtcctgca gaccgaacag
      361 tgcgaacgcg accgctttac cctgaccgtt agagatcagt acgccgttct gacgctgacc
      421 cacttcaccc ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc
      481 ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta
      541 gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc
      601 gcccagcgcc tgcattacag aaccctgatg ctgttcttca acgtccagag tcacgttttc
      661 atacggctct tgtttacgac cgtcgatttc acggaagatt acttttggac gggataccgc
      721 cagttcgaaa ccttcacgac gcatgttttc gatcagaaca gacaggtgca gttcgccacg
      781 accagaaacg cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg
      841 taccagttct ttgttcagac gatccaggat ctgacgagac gttacgaact taccttcttt
      901 accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga
      961 gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc
     1021 gccaaggccc gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc
     1081 cagaccgagg tggcccagca ctttaccgac tttcgcgttg cgggttttgc cttcgctatc
     1141 gatgatagtg acctgctggt tcggcttcac tttaccgcgc ttgatgcggc caatgccgat
     1201 aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag
     1261 gtcaacgtcc ggcgcaggaa cgtggtcaac aatcgcctgg tacagcgggg tcatgtcttc
     1321 cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg
     1381 gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac
     1441 aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag
     1501 gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc
     1561 gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa
     1621 gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt
     1681 gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg
     1741 ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccag
     1801 ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac
     1861 aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa
     1921 acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca
     1981 cggagcataa aaagggttat ccaaaggtca ttgcaccaac atggtgctta atgtttccat
     2041 tgaagcacta tattggtgca acattcacat cgtggtgcag cccttttgca cgatggtgcg
     2101 catgataacg ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt
     2161 acggcgacac ggccaaaata attgcagatt tcgttaccac gacgaccatg accaatccag
     2221 gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag
     2281 tttgttgatt tgcgcttcac cgatactaaa ggtaaagaac agcacgtcac tatccctgct
     2341 catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc
     2401 ggctggaaag gcattaacga gtccgacatg gtgctgatgc cagacgcatc caccgcagtg
     2461 attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc
     2521 accctgcaag gctatgaccg tgacccgcgc tccattgcga agcgcgccga agattacctg
     2581 cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc
     2641 gatgacatcc gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa
     2701 ggcgcatgga actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg
     2761 aaaggcggtt acttcccggt tccaccggta gactcggctc aggatattcg ttctgaaatg
     2821 tgtctggtga tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact
     2881 gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt
     2941 cagatctaca aatatgttgt gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt
     3001 atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct
     3061 aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg
     3121 tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc
     3181 accaactctt ataagcgtct ggtcccgggc tatgaagcac cggtaatgct ggcttactct
     3241 gcgcgtaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt
     3301 atcgaagtac gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg
     3361 atggccggtc ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac
     3421 ctgtatgacc tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa
     3481 gaagcactga acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcact
     3541 gacgaagcaa ttgatgcgta catcgctcta cgtcgcgaag aagatgaccg cgtgcgtatg
     3601 actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac
     3661 ccggcgcaac aacgccggga tttagttgcc gtggaaactt tcagcccatc tctgcatggg
     3721 cttttttctc cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc
     3781 actaaaatgg tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg
     3841 ctgggcagat cctcaactcg ctgattaaca gtattttgtt aattgatgac aatctggcga
     3901 tccattacgc caaccccgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg
     3961 gtacaccgtt accggaactg ttgagctact tctcattaaa tatcgagctg atgcaagaaa
     4021 gtctggaggc ggggcaaggt tttaccgata acgaagtgac gctggtcatc gacgggcgct
     4081 cgcatatcct ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg
     4141 ctccgatgga taaccagcgc cgcttaagtc aggaacagct acagcacgcc cagcaggttg
     4201 ctgcccgtga tttagtgcgc ggcctggcac atgagattaa aaatccgctt ggaggtttac
     4261 gtggcgcggc gcagctgctc agcaaagcgt tacctgaccc atcactactc gaatatacca
     4321 aagtgattat cgaacaggcg gaccggctgc gaaatctggt cgaccgtctg ttggggccgc
     4381 agctgcccgg tacgcgcgtt accgaaagta ttcacaaagt agctgaacgc gtggtgacgc
     4441 tggtgtcgat ggaactgccg gacaacgtgc ggttgattcg tgattacgac cccagcctgc
     4501 cggaactggc gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg
     4561 cgctacaggc gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc
     4621 aactgacctt acacggcgag cgctaccggc tggcggcgcg gattgatgtg gaagataacg
     4681 ggccgggcat tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg
     4741 aaggtggcac cgggcttggc ttatccatcg cccgtaattt gattgatcag cattcaggca
     4801 aaattgaatt taccagttgg ccaggtcata ccgagttctc ggtttacctg cctatcagga
     4861 aataaaggtg acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat
     4921 ccgttgggtg cttgaacgtg cgctcgctgg agcgggttta acctgtacga catttgagaa
     4981 cggcgcggaa gtactggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat
     5041 ccgtatgccg ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat
     5101 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca
     5161 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt
     5221 tgagcgcgct atcagtcatt accaggaaca gcagcagccg cgtaatattc agcttaacgg
     5281 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg
     5341 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga
     5401 actggtcgct catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct
     5461 gaatatggca gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg
     5521 cgcgtttact ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac
     5581 attattcctc gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt
     5641 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg
     5701 tattatcgct gccactcacc agaatctcga acaacgagtg caggaaggta agttccgtga
     5761 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg
     5821 ggaagatatt ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt
     5881 agaagcgaag ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg
     5941 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga
     6001 agtgttgatt caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc
     6061 gcaaatgcaa ccggacagct gggcaacgct tcttgcgcag tgggcagaca gagcgctgcg
     6121 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac
     6181 gaccgcgttg cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg
     6241 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt
     6301 gtaaagattg attattgagc gcaaattgct ggtattttac gctttactgt tccgataagt
     6361 tcagtatgat cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct
     6421 ggtatcgagt ggcgcagttg acagccacac accgcaaact gccgttgctg ccgtgctgtg
     6481 tgccgcaatg attgggctgt ttagttgagt ttcagattgc tgatgaagtg ctctttgttc
     6541 atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcaatatttt caacaggttt
     6601 tgtaggcctg ataagcgtag cgcatcaggc aatcttgttc tcagcacatt gatggcgctt
     6661 tcgttttcta ctttgtaaac gaagcgccat tcactattta aatcacccgt gagaactgct
     6721 gcatccgcgc tttctggcgc agataggtat caaagcacat gcaaatgttg cggatcagca
     6781 agcgaccttt cgccgtcacc tgtattccct tctcatccac atccaccagc ccatcttttg
     6841 ctaacggggc gagcagcttg agatcttccg caaagtaatc agcgaagtgc aaatcccact
     6901 gtttctcaat aggggcgtaa tccagacgga agttgcagat gagcgactta atcacatcgc
     6961 ggcgaataca gtcatcacgc gttagcgcaa taccacgcca cagcgcattg ccttgttcat
     7021 ccacttgctg atagtactgc ttcaactctt tctggttctg cgcgtagcag tcgccaatca
     7081 tgctgatggc ggaaacgccc atccccagca gatcggtatc gccctgagtg gtgtagccct
     7141 ggaagttacg atgcagcacg ccttcacgct gggccaccgc cagctcgtca tccggacggg
     7201 caaagtgatc cataccgata aactgatagc ccgattgcgt caggaaggcg atggtttcct
     7261 gcaggatatc gagtttttgc tgcggactcg gcaggtcagc atctttgatt ttgcgctgag
     7321 cagcaaaaat ggtcggcaga tgcgcgtagt taaagacact cagacgatcg gggttcagct
     7381 ccgccacacg tttcagggta aaggcgaaac tctccggcgt ctgtttcggc aggccgtaaa
     7441 tcaggtcgat gttggtggag gtaaaaccaa tctcacgcgc atggttaagc agtgcaaaga
     7501 tgaactcttc atcctgctcg cggttaacca gacgttgcac ttctttgttg aagtcctgca
     7561 cgcccatgct caggcgatta aagccttcgg cgcgtaaatg atcgagtaca tccagttcga
     7621 tttcccgcgg atcgacttcg atcgaaatct ccgcatcggc attgaactgg aagttttcgc
     7681 gcagcagctt catcaggcgg ctgatttgcg ctttattcag atacgtcggc gttccgccgc
     7741 cccagtgcaa ttggctgacg tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt
     7801 gctccagcgc gtccagatac tgatcggcct tgtgctgctg gcgagtaaca atcttattgc
     7861 aaccgcagaa gtaacaaagc ttatggcaga acgggatatg tacgtagaga gataatggac
     7921 gctcaggata gcgcgccacg gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact
     7981 ccagcgcggt cgggtacgag gtgtatcgtg gcccggaata gttatatttc tggatcaggg
     8041 ccagatccca gtcgatttgc tgtacagaca tgctcactcc ttccgatggc gtctctggcg
     8101 gctacggcgt accggcggat gcccgtttcg ggtcatcagc cggttgcgca gccacttttg
     8161 tcgccgcgac aaccgctgta gtttaacgaa taaccacacc agataacata taaccggaag
     8221 ggttatcagc agaacgggaa ggcccacggt ggcaatccgt tagttacccc gcagcagacg
     8281 catcatgtct tcttgcttct cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc
     8341 gagtttctgc atcaactcat caatacgatc cagtttagca tccacccagg attgctcttc
     8401 ggcgctcagg gtttcgcctg cttccagacg ttccagcagc gcatccagac gctcatccgt
     8461 ttccagtaat tccaactccg cctgcggtga aagcataggt ttctcactct tcggtttgtg
     8521 ctgtttggtg actttttcag ccacgcccaa tggaataggg gttttactgc caatacgtgg
     8581 atcttttggt gcgttctggc ctttgctgcc tgacgtggtg tttccgcccg ctgcgcggct
     8641 gcccggcgca tgaccacgac gttttttctg acgcttgcga tcacgagctt cctgatccag
     8701 ctcctcgcgt gtttttcgac gcgcttttgc atgacctttg ctgcgtgagt ttgaagatga
     8761 tggtttcata atggttcgtc ttttagccgt tttattcagt atagatttgc ggcggaatct
     8821 aacagaaagc aagcaaagaa aaaaggcgac agattactct gtcgcctttt ttcctgactc
     8881 ataaccctta acgggtctta caatccttgc aggccgccaa cacaccctgt ttttccttta
     8941 gccggaacaa tccgtgttga ttccatttcc gtttaattac gtctccggac gtttgtcttc
     9001 ctgacaatcc tctgtcttcg cctcctggcg ctcctgaccc tcatcctgag tcattgttcc
     9061 tgttagcgtc ctcgctctat gggtgctact ttacgccttt gctttaaaca aacaatcaac
     9121 cgccatcagt atttgcacat tttcagagtt ttatataaaa gcaattcact gatttatata
     9181 gcttatttga catgcatcgc agaattttct ctgctatttc gcataaagac tatcggcaat
     9241 gtctcacaaa gcgtatggct tttacttaca acgctcacgc cgaaaaatcc gccttttatt
     9301 atggttcagg tataatcgcc acaaagcttt gactgacgga gacgaccgct ttgactaatt
     9361 tgaattatca acagacgcat tttgtgatga gtgcgcctga tattcgccac ctaccttccg
     9421 ataccggaat tgaagtggct tttgcaggcc gttccaacgc aggtaaatcc agcgcgctga
     9481 acacgctgac taaccagaaa agcctggctc gtacctcaaa aaccccaggg cgcacccagc
     9541 ttatcaacct gtttgaagtg gctgacggca agcgtctggt tgacttgcct gggtacggtt
     9601 atgcggaagt cccggaagag atgaagcgca aatggcagcg tgcgctcggc gaatacctcg
     9661 aaaaacgtca gagcctgcaa ggtctggtgg tgctaatgga tattcgccat ccgctgaaag
     9721 atttggatca gcagatgatt gagtgggcgg tagacagcaa tatcgccgtt ctggtgctgc
     9781 tgaccaaagc ggacaaactg gcaagcggcg cacgtaaagc gcaattgaat atggtgcgtg
     9841 aagctgtact ggcgtttaac ggtgatgtgc aggttgaaac gttttcttcg ttgaagaaac
     9901 aaggcgtgga caagctgcgg cagaaactgg atacctggtt tagcgagatg cagcctgtag
     9961 aagaaacgca ggacggcgaa taattttctt gccttaatgc ttgtgccgga tgtggcgtat
    10021 ccggcccgta aattcaatag attacaattc taccgttggc ctgataaaca tagcgcatca
    10081 ggcattacgg atcttttctt tcgcccaata aaaaacgccc cagtcattac tgactggggc
    10141 ggctaaaata ttcagccaaa tccgattacg tgaagtaaaa ggtctgaaag atagaacatc
    10201 ttacctctgt accctacgcg actaacttta ctcttttttg ttcagttcag aaagcacttt
    10261 ttgtaatttt ttttcagttg ttgcatagga atttctaata gccatcacaa aacgtgacag
    10321 cttatgttgc ttacttacga aaaaagggca tgttcaggcg aatcttagtg cgcctgatcc
    10381 cagttttcgc cactccccac ttccaccagc aacggcacat ccagacgggt acagttttcc
    10441 atcagttgat gaatctgctt cgcgacggca tcaacatcat ctttatgaac ttcaaatacc
    10501 agttcatcgt gtacctgcat gatcatacgt acacgcggtt gctcagcctg taaccacgca
    10561 tcaacggcaa tcatcgcccg tttgataatg tcggcggcgg ttccctgcat tggcgcgtta
    10621 atggctgcac gttcagccgc tgcacgacga gcaccattgc tggatttgat atccggcaga
    10681 tacagacggc gtccgtccag cgtttcaacg tagccctgct ctttcgcctg agcacgggtg
    10741 cgttccatat actccagcac gccagggtag cgttcgaagt aaaggtccat gtacttctgc
    10801 gcttctttac gtggaatgtt caattgccgc gccagaccga aagcactcat gccataaatc
    10861 agaccaaagt tgatcgcttt cgcgctacgg cgttgctcgc tggtgacggt ttccagtggc
    10921 aaaccaaaca cttctgccgc cgttgcccgg tggatatctt ttccttccgc gaatgcggtc
    10981 agcaagcctt tgtcacgcga aagatgcgcc ataatgcgca gttcaatctg cgagtagtcc
    11041 gctgagacaa tcacataatc ctctggcgca ataaacgcct ggcggatacg acgaccttct
    11101 tcgttacgca ccggaatgtt ttgcaggtta ggatcggttg acgataaacg tcccgttgca
    11161 gttactgcct ggtgataaga ggtatgcaca cgcccggttt tcgggttgat catcagcggc
    11221 agcttgtcgg tgtaggtcga tttcagcttc gccagaccac gatactccag aatcactttt
    11281 ggcaacggat agtccagcgc cagttcttcc agtacctctt ccgacgttga cggcgcgcca
    11341 cccggcgttt tcttcagcgg tttaatgccc tgtttttcaa acagaatggt ttgtaactgc
    11401 ttggtggaag aaaggttaaa ttcctcacct gcaatttcat gcgctttctt ttccagctca
    11461 gccagacgaa gggtgagctc ttcagaatga ttgtgcagca ctttcggatc gatcttcaca
    11521 ccgttacgtt caatgcgtga aagcaccggc accagcggca tttcgatatt ctcgaagacg
    11581 ttcaacggcc ctttgtgttt ttgcagatct ggccacattt tcagatgcaa ctgcaaggtg
    11641 acatccgcat cttcggcggc gtaacgtccg gcctcttcga gggcaatctg gttaaaggtc
    11701 agttgatttt tgcctttacc cgcaatctct tcaaaagtga tagttttgtg cttcaaccaa
    11761 cgttccgcga ggctgtccat atcgtgacgc ccggcaacgc tattgagaat gtaggactcc
    11821 agcatggtat caaacgcaat cccacgcagc tcaatgccgt aattcgccag aataccgcga
    11881 tcgtatttca ggttttgccc gaccttcagc gccttttcat cttccagcag cggttttagc
    11941 aactcgagtg cacgctcgcg agagatttga tcgggcgcat caagataatc atgagcaacc
    12001 ggaatatatg ccgctacgcc tggctcgata gcaaaagaaa gcccgaccag gttagcagag
    12061 atgttatcaa ggctgtcggt ttcggtatca aatgcaaata ccggcgcttt ttccagcttc
    12121 gcaatccacg ctttcagtgt ttcttcatca aggatggtga cgtagttgtc ataagaaatc
    12181 accgttgccg tcacttctgg cgcttcgtct gcaacactgg tttcctgcgg cttcgcggct
    12241 ggttttgccc ctttggcctg taaccatttg cccgcttcga catcagcagt ccagcgtttg
    12301 aactcatact ttttgaacag ccccaacaac tcttccgctg ccggttgctg cacttccagt
    12361 tgttcacagg tcagctccag ttcaacgtcg gttttaatcg tcgccagctg gtatgagaga
    12421 taagcaactt ctttgttttg ctcgagcttc gctgccattg ttttcgcgcc acggaagctc
    12481 aacccagcaa ttttttctgg ctcggcatac agcgtatcca gcccgccaag accttgcagc
    12541 aatgcctgcg cggttttttc accgacgccc ggtacgccag gaatgttatc ggaggagtca
    12601 cccatcagcg ccaggaaatc gatgatcagt tctggcggca cgccgtactt attcaccacc
    12661 tcttccggtc cgaggatggt attcgtcatg gtgttgataa gcgtaatatt tggcgtcacc
    12721 agctgcgcca tatctttatc gccagtgctg atcagcaccg gacgcccggc tttttcggct
    12781 tcgcgcgcca gagtaccgat aacgtcgtcc gcttctacgc cagaaaccgc cagcagcggc
    12841 agtcccatcg ctttaaccat cgcgtgcagg ggttcgattt gtgctcgcag atcgtccggc
    12901 attggcgggc gatgtgattt gtaatgttca aacagttcat cacgaaaggt ttttcccttg
    12961 gcgtcaaaga ccaccgctgc atgcgtcggt ttatattgca tgatcagact gcgcagcatg
    13021 ttgaggacac catacatcgc accggtcggc tcgcctgcgc tgttagtcag cgggggaaac
    13081 gcgtgatatg cgcgataaag ataagatgaa ccatctacaa ggataagtgg attttggggg
    13141 atctgaacca taatgtccgt gcctgtttat cagattatgg gtaaaggatg ccacagacag
    13201 gatgaaaaca tgagtttttc accgcttttc gtgaaaatct tttgtagatc ttctggatcg
    13261 ctcgcaaatc gtcatgtgga taactttgtg cataaattat ttacatcgct tttaatttat
    13321 tatcatgctt tttacagcat gatgtttgtc tttgtttttc atgcagttaa aaataaagcc
    13381 atccctaata attgagataa tgggattttt tctaatgtgg atagatatgg attatttttc
    13441 tccttaagga tcatccgtta tttgggtcgt tttctgctaa aatctgcgcc ttgcgcttct
    13501 ttagcgcact gtctatggct aaccttttga ataaattcat tatgacgaga atactcgctg
    13561 cgataaccct tttgctgagt atcgtattga ccattttggt cactattttt tgttccgtcc
    13621 cgatcatcat tgccgggatt gtaaaactat tgctacctgt gccagtcatt tggcgaaagg
    13681 tttcgcgttt ttgtgatttt atgatgtatt gctggtgtga aggtctggcg gtattactgc
    13741 atctgaaccc acacctgcaa tgggaagttc acgggctgga agggttaagt aagaagaact
    13801 ggtatctgct tatttgtaac caccgtagtt gggcagatat tgtcgtactg tgcgtgctgt
    13861 ttcgtaagca cattccgatg aataaatatt tcctcaagca acagctggcc tgggtgccat
    13921 ttcttggcct ggcgtgctgg gcgctggata tgccgtttat gaagcgttat tcccgcgctt
    13981 atttattacg tcatccggag cgacgtggta aagatgttga aaccactcgg cgttcttgtg
    14041 aaaagtttcg cctgcatccc accaccattg tcaatttcgt tgaaggctcc cgtttcacgc
    14101 aagaaaaaca tcagcaaacc cactccactt ttcaaaactt gttgccacca aaggcggcag
    14161 gcattgcgat ggcgctaaat gtactgggta aacaattcga taaactgctt aatgttacgc
    14221 tgtgttatcc ggacaataac cgtcagccgt tcttcgatat gttaagcggt aaactgacgc
    14281 ggattgtcgt acatgtggat ttacagccta ttgccgatga gttacatggc gactacatca
    14341 atgacaaaag ctttaagcgc cattttcagc aatggctgaa ctcattgtgg caggaaaaag
    14401 accggcttct gacgtctctc atgtcatcac agcgtcagaa taagtaagtt ttactcgcct
    14461 ctgaatgagc agaggcgagt gagtatttta atgaacaaaa cgtccggcac gagacataaa
    14521 ttcttcttcg ctcatctcct gtccgttaaa cacaacttta cccggtgtat aacgcagctg
    14581 cagcgaggcg gtattgttgt caatcgtgat taactgaaac atctgaccca atgtcatcat
    14641 cccgctgatt tgtttatcag cctgcttttg cgctttttcc gcatccattc cttcagataa
    14701 attaagctgt tttgcggttt ctgtgacgac attaagcggt aacttcacat caaaattgag
    14761 caatttgata tctttgtttg tggatgatga agatttggct gggtcggcaa tactgatatc
    14821 cagattggca tttagttcgc cgagtgcgtt cttccatctt accggttgtt taatggtcgg
    14881 ctcacttttt tgtaataacg gtaaatactc tttgaacaat gcagcattca cttcttgcag
    14941 agcaacttca tcgtttgcta actcagggtg tacagcaagc tgcttctgca tagcaatgtt
    15001 atactggatg ataaattggc gcactgctga gggatcaata gattcagcaa tcaatgacca
    15061 ctcaccacta ccgagttgct gattcccttt tgttaactta tcaagttcgt aggtcagatt
    15121 gacattgata taatctttgt ctttaacgcg atccagcatg gtttttgcaa cgaaagcatc
    15181 gatttgggca acgtcttccc cgtgattgat aatgttaatc tgattaaatt tcgactcgct
    15241 ttcaccaacc ggaaatttct tttcttctaa tcgcgccaga gaatcaatgg taaaactttt
    15301 tgccgttact ttgttcattg gtgataattg cagaacaata tcttcgactt tgccttcaat
    15361 atccaggtta gacaatgaac taccatcacc attaaaagta aactggccct caccaaaaga
    15421 aaactttcca tactctgcag gggcaacatc taatgtcgta gagaatgagc caccaaacgc
    15481 gaatcgcaat gtcgcttcca cgggcgattt atttttcgcg gcgataaata gcggttgtgt
    15541 cagttcatta ttcactaagt tcacttttgc cgctgccagt gctgggatga cattaccatg
    15601 cattaacatt gtgatgggca acggaccgtg ctcaacatcc acatcaaata caactttctg
    15661 gcctggcttg atattgagat cgggtgcacc gttatcgaat gtcatttgca tttgaaaacg
    15721 agaattaaaa aaacctttgt caaaattttt ataacttaac gtcatatcgt aggcatgttc
    15781 acctttcttt ttagcatcgt taaaatcttt aataaacttt tcgacaccag gctgaatttg
    15841 cgtacctgtg taccatgtgc caccacccca gattacggct aacgcaacaa taacacctgt
    15901 agctgactta cgtatcatat aaatattccc tgataaaaca gcaaaacttt aaatatccgc
    15961 ttatgctgaa ataatgtcca gcggcaggac gcattatcag aatgtattta tatttattaa
    16021 ataataaaaa aaaccgtgaa tattcacgg
//
LOCUS       DAZPLM010000085        16004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPLM010000085 DAZPLM010000000
VERSION     DAZPLM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.085"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(87..239)
                     /locus_tag="ACHVC7_002296"
     CDS             complement(87..239)
                     /locus_tag="ACHVC7_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2116331.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(504..1238)
                     /gene="cysH"
                     /locus_tag="ACHVC7_002297"
     CDS             complement(504..1238)
                     /gene="cysH"
                     /locus_tag="ACHVC7_002297"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR2116332.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1313..3025)
                     /gene="cysI"
                     /locus_tag="ACHVC7_002298"
     CDS             complement(1313..3025)
                     /gene="cysI"
                     /locus_tag="ACHVC7_002298"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR2116333.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3025..4824)
                     /gene="cysJ"
                     /locus_tag="ACHVC7_002299"
     CDS             complement(3025..4824)
                     /gene="cysJ"
                     /locus_tag="ACHVC7_002299"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR2116334.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5143..5505
                     /gene="queD"
                     /locus_tag="ACHVC7_002300"
     CDS             5143..5505
                     /gene="queD"
                     /locus_tag="ACHVC7_002300"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR2116335.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5583..6854
                     /locus_tag="ACHVC7_002301"
     CDS             5583..6854
                     /locus_tag="ACHVC7_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2116336.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6845..7105
                     /locus_tag="ACHVC7_002302"
     CDS             6845..7105
                     /locus_tag="ACHVC7_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR2116337.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7122..7697
                     /locus_tag="ACHVC7_002303"
     CDS             7122..7697
                     /locus_tag="ACHVC7_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR2116338.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7845..8705)
                     /locus_tag="ACHVC7_002304"
     CDS             complement(7845..8705)
                     /locus_tag="ACHVC7_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR2116339.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8702..9481)
                     /locus_tag="ACHVC7_002305"
     CDS             complement(8702..9481)
                     /locus_tag="ACHVC7_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR2116340.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9459..10409)
                     /locus_tag="ACHVC7_002306"
                     /pseudo
     CDS             complement(9459..10409)
                     /locus_tag="ACHVC7_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10396..11829)
                     /locus_tag="ACHVC7_002307"
     CDS             complement(10396..11829)
                     /locus_tag="ACHVC7_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR2116341.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11899..12715)
                     /locus_tag="ACHVC7_002308"
                     /pseudo
     CDS             complement(11899..12715)
                     /locus_tag="ACHVC7_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13033..14310
                     /locus_tag="ACHVC7_002309"
     CDS             13033..14310
                     /locus_tag="ACHVC7_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR2116342.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14337..15823
                     /locus_tag="ACHVC7_002310"
                     /pseudo
     CDS             14337..15823
                     /locus_tag="ACHVC7_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3765 a         4222 c         4035 g         3982 t
ORIGIN      
        1 ttcacataaa taatataaat taaaaaaggg tgcaggagac caaaccatca caattcagta
       61 cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag aactgcctta
      121 aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac catcagcgtg
      181 aatcccagta ctgtaaaaca cagcactatg attgccacaa gggcatattt tgtcagcatg
      241 tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga acgctggcct
      301 caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg aatgcttaac
      361 gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc agcggcgttc
      421 acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag
      481 gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct ttttaagcca
      541 aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt atgggtatca
      601 ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg tttttgcagg
      661 tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa tacgccacgc
      721 tgaattgcca gcaccggtaa attggcgcga ctgccggatt gttcacggcg caggccagca
      781 aaccaggttt gcgcgttaag ttctttcaga gcccggttca tcggttcgac tttgttgatg
      841 tcattgtact tttcaatgcc ttcaacgccc tgctcccaca gttttccgta gcgtgcttcc
      901 tgccaggctg cgctttcggt agcacggtac actttcaggt tgagcttgag tttgtccgtt
      961 aactcgtcaa taaagcggta ggtttccggg aacaagtaac ccgtatcggt gaggatcacc
     1021 ggaatatccg ggcgaatttg attcaccaga tgcaggctca ccgccgcctg aatgccaaag
     1081 ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggctac gcggccttca
     1141 gcgtccagtt tttccagttc ggcgttagtt tccgccagcg ccagaatacg atctaccttc
     1201 ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact gtttgccttg
     1261 cctgatgcga cgctcacgcg ttcttatcag gcctacaaga ccgggctgat ggttaatccc
     1321 acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc
     1381 cgaagccttc acccgcttcg cgctctttcg cccagcgccc tatcagttca tcaagcgacg
     1441 ccaggatttc cggctcggtg atgttttctt tatacatccg tgggatacgt gtcccaatgc
     1501 ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt acccaccagg cccacttccg
     1561 ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac acgcatcacg atatgctcat
     1621 cgctgacacc atgtttcgcc attaaattat cgatgttgtc gataaaagac ggcaggaaac
     1681 gctctgcttc cgccatcgcc agcgggcaag tcgggaatga cacgcaggcc atcgagtttt
     1741 cacgctgcgg cgtgacggca ttcattaacc cgctctcttt ggcgatcttc tcgatcttcg
     1801 ctttctcgct ttccggtaca ccggtgatga tcagattctg gttcgccgta atgcggaaat
     1861 cgcctttgtg gatcttcgcg atctccagca ggccggtttt cagcggacgc cccggataat
     1921 caaggatgcg accattttcg ataaacagcg tcaggtgcca gttatcatca atgcccttaa
     1981 cccagccaat acgatcgcct cgtccggtga actcatatgg acggatcggt tcaaatttga
     2041 tccccgcgcg acgctccact tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt
     2101 atttggtttt ggcattttta cgatcggttc ggttacccca gtcacgctga gttgtcacga
     2161 cggcttcggc caccgccagc gtatgctcca gcggcagata gccaaactcg ctcgccgtgc
     2221 gggcgtaggt tttcttgttg ccgtgttcga tagaaagccc accgcccacc agcaggttga
     2281 agcctaccag cttgccgttt tcggcgattg ccacgaagtt catgtcgttg gcatgcagat
     2341 cgatatcgtt ctgcggcggg atcactaccg tggttttgaa cttacgcggc aggtaggtct
     2401 gaccaaggat cggttcttca tcagtagtgg cgactttttc ctggtcgagc cagatctccg
     2461 catacgcgcg ggtacgaggc aacagatgct cagaaatctt cttcgcccac tcgtacgctt
     2521 ccgcgtgcag ctgcgactcg taagggttcg aggtgcagag tacgttacgg ttcatgtcgt
     2581 tagctgtcgc cagcgcatca agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt
     2641 tctttttcag aatgccgtgg aactgaaacg tctggcggtt ggtcaggcga atgctgccat
     2701 agatggtgtt ttcaccggca aatttgtcga tcgcctgcca ctgtttagtg gtaatcaccc
     2761 cacccggcag acgacagcga agcagcatcg cgtggcgcgg ctccagcttc tgttcagcac
     2821 gttcggcacg gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg atcagcagga
     2881 agttgtcgcc cttaaagccg ccggtcagac cgtcgtttaa atcttccgca atggtgccgc
     2941 gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt cagttttcct tcgaccacta
     3001 aaggccctgg atgtttttcg ctcattagta gacatctcgc tgataacggc gctctacgcg
     3061 cagctcactt aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt cggcaatcac
     3121 ttccagaagt gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc agacataaat
     3181 gtgggcaccg tcattgatcc agcgccacag ctccgcgccc tgttcgcgca gtttgtcttg
     3241 tacataaact ttttcttttt ggtcgcgcga ccaggcaaga tcgatacgcg tcagcacgcc
     3301 ctctttgacg tagcgctgcc actccacctg gtagaggaaa tcttccgtaa agtgcgggtt
     3361 gccaaagaac agccagtttt tacccggcgc ttcgtcggcg gcgcgttgct gcataaaggc
     3421 acggaacggc gcgatgccgg taccggggcc aatcataatc actggcgttt gcgggttagc
     3481 gggcaggcgg aaattgtcgt tatgttcgat aaatacgcgg acttcgccct cttcttccac
     3541 gcgatccgcg aggaagctgg aggcaccacc ggcacgggcg cggccttcca cgtcgtaacg
     3601 caccacacca acggtgacgt gtacttcgtt ctcgacttcc gcctgcgagg aggcgatgga
     3661 atacaggcgc ggggtcagcg ggcgcagcag gttaatcagc gcttcggcat ccagttgcgc
     3721 cggagagaaa cgcaccatgt cgacaatcgg cgtggtcgcg gcgtaatgct gtaacttcgc
     3781 tttatcgccc accagcggca gcagcgtttc gctgcgggta agcgtggcat agttctcaac
     3841 aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt tcagaggcaa
     3901 cgttttgccc tcgacggtga caggttcatc gcctttcagc cacagcagtt cgacaagttc
     3961 tttcaccagt gccgggtcgt tctgatacca gacacccagc gcgtcacccg gctggtaacg
     4021 caggcctgaa tcgcctaagt caatttcgat atgacgaacg tctttttcag agttacgccc
     4081 ggtaattttc tggttaaccg aaaggctcgc caccagcggc gcgtctttgc tgtacgggct
     4141 ggtgtggatt tcatttaccg cgccagtagc gacggattgc gaaggtgccg cgacaggcgc
     4201 acgcgattta agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag cctggtattc
     4261 aacatcggca tcgacacggt cgagcaggcg ttcaccaccc agttccgcca gcttgctgtc
     4321 gaaatctttc ccggactggc agaaaaattc ataagagcta tcgccgaggc taaacacggc
     4381 aaacgcggtg ttttccagct ttggcgcttt tttggagaac aggaacttat gcagcgcgac
     4441 ggcttcttcc ggcggttccc cttccccttg cgttgacgtc actacgatga gcagtttttc
     4501 gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt tcagttttgc
     4561 tgctaataaa tcatcgcgta atgcttcagc aacccggcgc gcattgccgg tttgcgaggc
     4621 ggagataata gttatacccg gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc
     4681 aggctgctga ttgagtacgc cccagaaata gccagaaacc caggcaagct gggtaggcgt
     4741 taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc
     4801 ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc
     4861 gccctgtcag cgcccataaa acagaagaga agataaggtt aacggggtga acggtgtgga
     4921 ttaaagacgg gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct
     4981 ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa tgaatttagc caatcattaa
     5041 gataaatcag cgattttgcg caacaaagtc gttttagata atgcgaaaaa acagcctttc
     5101 cggtactcta cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt
     5161 aaagatttca ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt
     5221 ggtcgcctgc acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg
     5281 catacgggct ggattatcga tttcgctgaa ctgaaagcgg cgtttaagcc aacttatgag
     5341 cgcctcgatc accattatct caatgatatt ccaggtctgg aaaacccaac cagcgaagtt
     5401 ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg
     5461 gtaaaagaaa cctgcaccgc agggtgtatc tatcgcggcg aatgataaga gtgtgtcggc
     5521 ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg ttctggatat ctggggcatg
     5581 acatggaaga cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg
     5641 cgttacgctg cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg
     5701 gcagcaaaaa tctttccggc gggcggttat atacccatgc actcgcggaa ctcctcccgc
     5761 aatttcatct gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa
     5821 cgcccgatgg cgtaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat
     5881 tacgtgcacg gttcgatccg tggctggttg ccgaagccga aaaagaaggt gtcgaatgca
     5941 tccccggagc gacggtggat gcactgtatg aagaaaacgg cagagtctgt ggcgttattt
     6001 gtggtgacga tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc
     6061 tggctgaacg tcacgggtta gtgactcgtc ctgctggcga agcgatggcg ttggggatca
     6121 aagaagtgct gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaataacg
     6181 aaggcgcagc gttgctgttc agcggcggaa tctgtgatga cttacccggc ggcgcatttc
     6241 tttatactaa tcaacaaacg ctctcgttag ggattgtttg cccgctctct tcccttacgc
     6301 aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa agcgcatccg gcagtgcgcc
     6361 cgcttatcaa aaacacggaa tcactggagt atggtgcgca tctggagcca gaaggtggct
     6421 tgcacagtat gccggtgcaa tacgccggta acggctggct gctggtgggc gatgcgttgc
     6481 gcagttgcgt caataccgga atttccgtgc gcggcatgga tatggcgctg actggcgcac
     6541 aggcggcggc acaaacgcta ataagcgcct gccagcaccg cgagccgcaa aatctgtttg
     6601 cgctttatca tcacaacgta gagcgcagcc tgctatggga tgttctacaa cgttatcagc
     6661 atgttccggc gcttttgcaa cgccctggct ggtatcgggc gtggcctgcg ttaatgcagg
     6721 atatttcccg cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttat
     6781 tctggcgtca tttacgtcgt catggcctgt ggcatctggc gggcgatgtt atcaggagtc
     6841 tgcgatgtct gtagcccata atctctggcg cgttgctgat gcgccgcaca ttgttccggc
     6901 tgactccgtt gagcgtcaga tggtacaacg gttgattaac gcctgtccgg caggtctttt
     6961 ttcgctcaca ccggaaggtg acttacgcgt tgattatcac ggctgcctgg agtgtggcac
     7021 ctgccgtttg ctgtgcgacg aatccacact acaacagtgg cgctatccgc cttccggatt
     7081 cggcatcacc taccgctttg gataaataat aaggataatt tatgcccctc ttacacctac
     7141 tccgccagaa tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggctatcg
     7201 attctgaatg ccagtttatt tccgtgttgt acggcaatat ctgcaccatc agtaatatcg
     7261 ttaaaaagat taagaacgcc gggaaatatg cttttattca tgttgatttg ctggaaggcg
     7321 cgtcaaataa agaagtcgtt attcagtttt tgaaactggt gaccgaggcg gacggcatta
     7381 tcagtaccaa agcctcaatg ttgaaagcgg caagagcgga aggttttttc tgtattcatc
     7441 gcctgtttat tgttgattca atttcgtttc acaacattga taagcaagtt gcgcaatcga
     7501 atccggattg tattgagatc ctgccaggct gtatgcccaa agtgctgggc tgggtgacag
     7561 agaaaatccg ccaaccgctg attgccggtg ggctggtgtg cgatgaagaa gatgcgcgta
     7621 atgcgattaa cgcgggtgtc gtggcgcttt ccaccacgaa taccggggtc tggacgttag
     7681 cgaaaaaatt actttgacgg gataatcgta accaattgaa tttggtttga tttgtaggcc
     7741 gcacgccaca tccgacattc agcgcctgat gcgacgcttg acgcgtctta tcaggcctac
     7801 aagtccccag gccagaaccg taggcaggat catccggcaa cacgttattg gcaatctgcg
     7861 tgaatgtttg tcaccagtgc ctccagcacc actttccagt catcaaccac gccaacatct
     7921 gcctgtgaaa ataccgcagc gctggcgtca tgattaatcg ccaccacaaa cttgctattg
     7981 cgtacgcccg ccattaacgc tgctgcgcca gaagcgccaa caacaataca cacctcaggt
     8041 gccagcagat gtccggaaat accaatcact ttttcagcat caacgccacc gttcatcact
     8101 cgcgcccgac tgtagcccac ttcggctccc agcttttctg ccagcatagc gatttcctgg
     8161 ttatctgcct ctccgccctg ccccaccacc agcacccgtc tggcctcagc taatgggtcg
     8221 cgagtaacat ttttcagatc ctctgtgcta accagccagt ccggaagcgc acccgggaca
     8281 atgttcagtt gttgcattcc agagggcaac gtggcgtttt tcgctgctcc cgcctgacgc
     8341 gccagggaaa ggcatagcgg gcgtttttct gtttgcagcg tcgccgttag cgcatttccc
     8401 cagtgggatt tgcgcacgct tacggttgga atatctaacg aagtcacctg gcagatgctt
     8461 gcaccatgta aacgccaggc cagccgggtc gacagttcat cgccaaacgt tcccggcgga
     8521 aagagaacaa cgtccgcagg cgttcgctgc cactgttcca ccaacgcatc cagcacctgt
     8581 tccgccacta caggctgagg ttcaatctgc cagtgcgcca gggtacaacc gctgaaatcc
     8641 tgcgccgcca gccagcttgc tattgcggca ttctcctgat ttattgtcac aattgcgata
     8701 ttcatggctg catcctttgg cgtaaatagt cctgccatag cttttgagct ttttctgcta
     8761 ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc agttgcagac
     8821 actgcatcgc tggcatttcg gcagcaacag ttttacggat aatttctgct ttccccgcag
     8881 ccattcgctg gcgcattccc ggtactggca aggccacttc accgcactga cgcacggcaa
     8941 tcaccgcagg caagcgaacc cggcaacaac gcaacccatg ttcagtacgc tgttcgaggg
     9001 tgataaacag cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc cagcccagca
     9061 tttccgccag caaaaatggc gtttgcccat tctgcccttc gctgctttgg cagccggtaa
     9121 ttatcagatc cagcggattc tgatgctgcc attcggcaat atggcgagcg acaaattccg
     9181 gcgcaaagcg cagatctgcc gccgtctcca gcaatacagc ttcctcaaac ccaagagcca
     9241 tgagatagcg cagccagtgc aatgcccgtt catcccccat gctcaacgcg gttaaagaca
     9301 tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc agcggcctgt tcatcagcac
     9361 cgagtaaact tcgcagtagc gaaatatccg gtccgctttt accctgagcc gccgcctgcc
     9421 actctttttc cgccagcatt ccggcatccg gttcggcttt aaacgctaac agaatgttca
     9481 ttcgcacctc ctacatttcc cgccgccacc agcgggaggg ctttagtttc tggtgcccat
     9541 agccaggtca caacaaaatc aaccaacaac actgtcgcca ggagcaataa ggtgacttgc
     9601 attccccact gcgccagcac ccacggcagc aggccagtac ttaccgccgc gccaagtcga
     9661 ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa actttccgca
     9721 ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg tgctgaagag aacaaaaagc
     9781 agcagcgtta atgaactgcc ggaaggcaaa caggccatca ctaccagcgt tgccgccagc
     9841 agcaaaaaac ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag aactaatccc
     9901 agcagcgcgc ccacaattaa caacgcatta agcatcaggc tggcagtcag cgcatcttcc
     9961 agaccaatag tctgggcgat agttggcagc caggtataaa tcacaaacca tgggattacg
    10021 aggcagacaa agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga agagaacaag
    10081 gttttgatgt gtttatgggt cgccgttacc acttcatcgc ccagtaaaac atggggacca
    10141 aaatagcgat gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag tagccagcgt
    10201 ggtgattctg gcgttcccca gcgtaataac gtaatcaaca acgcgggcag agctgccgat
    10261 gccagtagcc agcgccaggc ctccgggttt tcggaaataa agtgatgtcc ggcaatactt
    10321 gccagcacat agcctacggt ccacaccacg ctgaatgcgc ccagcaaaat accgcgatgg
    10381 cggcgcgggg aaaattcagc cagcaaggtt aatgccatta ggatcgaact gctttttcag
    10441 cccttccagc aacgcccacg cgctgccgtg ttccagtttg ctccagtgaa cgcgatgttt
    10501 accgataccg tggtggtgca ccatcgaacc gccgaggcga atggtttctt cacagatgat
    10561 cttgttgagc ggattgtggt acttgtcgat ttcctcttcc ggcttacagt caacgacgtt
    10621 gtaatcgtag acgaagtaca tgttggtgcc gttctgatag ctatgagagg aatgaccgcc
    10681 cagcatggtg atgtcgtcgg cgtgcgggaa ctcagtacga atacggttaa taacgctttc
    10741 gtagatttcg tggatgcagc tccagcagcc ggacacttca gtggtaaagc ccatgttgcc
    10801 ggttttgagg atctgcacac gttcggcagc cactttatcc ggtccccagt tcaggttgtt
    10861 aaaccaggtt tcgatcagct tgctgtccac gcgctggcat tgcgggtagc gggcaacgat
    10921 ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg ttaccttcag ccataaagat
    10981 cagcacgcat tttccgtctg caaaatgggt gaagtgttgg gtgccatctt cagcgtcata
    11041 caaacgagcg atcgacggac gatacccttc caccatgact tcacgcagga tgttgaagtc
    11101 ggttttcatg tcttccagga tatagccgta gaagaggttg ttttccgggg tgaatttaaa
    11161 gattttcact gttacttcag tgatatagca aagtgcacct tcgttaccga tgatgatgtg
    11221 gcgaatgtcc gggccagccg cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc
    11281 agccaatact gcttccagac caacgaccat atcttcgatt gcgccgtaga gtgtggagaa
    11341 ctgcccgata ctgcgggttg ctaccaagcc gcccatctgc gccagcggct ttgactgcgg
    11401 agaatgcccc gtggtgtaac ctttttcacg caacgcattt tccagcacct ccagcggaac
    11461 gccacattgc gccgtcgcct gcatattctc aatatcaata ttaatgattt gattcatggc
    11521 ggagccgtca agcaccaccg agttttccac aacggtttcc agcccacctt cagtggcgga
    11581 agcgccggta cgcggcacac cgttaatttt gtgcgcattc ataaaattca gcacacggga
    11641 tacttgctct gtggaaccga gttttacgac cgctgccgga atcggcaaag tataaatgcc
    11701 atgaatatcc ggaaatttac gaaaacggtc aatactgttc ttctttaata ctgtttcatc
    11761 ggtaattacg cgatctgcac caacaatttc ctttagttgg tcgacaatcg ctgcgcgaga
    11821 taaagacata gtaattcctt cctgataata aaaatggaat aatcattaat aagagaaaac
    11881 agaaaccacc gttatggatt agcgcactaa ataaccacca ttgaccacta ataaatgccc
    11941 attgacataa tttgaagccg gacttgcgag gaatacagct gcgcccatta aatcctgagt
    12001 atcgccccaa cggtttgccg gaatatgatc aagaacgcgc tgattggttt ctggattact
    12061 gcgtgtcgcc agcgtaatat ctgttgcata atagccagga gcaataccat ttacctgaat
    12121 attatattga cctagttcat cacaataagc tttggtgaat ccggcaagag catgtttagt
    12181 ggcagaatat gcaggtgacc attgtccacc taagtaagag aacaatgaac agatattaat
    12241 aattttgccg cttttctgcg ggatcataat ttttgcagct tcatagctta actcgaatgc
    12301 ggcggtcagg ttcacatcaa tcatcggatc ccagtcggca cgaccgaagt ccagcacctt
    12361 attcagctta caaataccgg cattgtttac cagaatatca actgtaccga aacgctcaca
    12421 gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg atatccacct gcatgaagtc
    12481 cacctcaaca ccctgttttt caaacatttc ctttgtttcg ccgttatctt tcaatcattt
    12541 cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg ccagctttgg
    12601 ccaacgccat ggcaaatgcc tggcctaaac cgctattccc accggtaaca attgcggttt
    12661 tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta
    12721 gctccaattt ccgtaaggca aaaaaaagaa aggtaacttc ccccgcgaga gggaagttac
    12781 ctttctcatt atctctggta actggagata atttttagca taacggcata actgctaatg
    12841 tgatcgtaat cacagtgtga tattcgtatt gtcaaatagt tttaaaaagc agaagcgtga
    12901 ttactctcac acaacacctc tgctttccat gttatttctt attgcaatta ctagagacct
    12961 gagaaaagta atccacctga aagggcggct tacttttctc ttttttttgt ctattacaaa
    13021 tgggaatacg atatgcaaca caactcatat cgccgttgga taaccctcgc gataattagt
    13081 tttagcggcg gcgttagttt tgacctggct tatttacgtt atatttatca aattcccatg
    13141 gcgaaattta tgggattcag caataccgag ataggtttaa taatgagtac ctttggtatt
    13201 gcggctatta ttctttatgc ccccagcggc gttattgccg ataaattttc acaccgcaaa
    13261 atgattactt ccgcgatgat cattaccgga ttactgggtc tgttaatggc aacgtatcca
    13321 ccgctgtggg taatgctctg tattcaggtc gcctttgcga taacgacgat tttaatgctg
    13381 tggtcggtgt cgattaaagc cgcctcgttg ctgggcgatc atagtgagca agggaaaatt
    13441 atgggctgga tggaagggct gcgcggcgtc ggtgtaatgt cgctggcggt gtttaccatg
    13501 tgggtctttt ctcgctttgc accggatgac agcgccagcc tgaaaacagt cattatcatc
    13561 tacagtgtgg tttacatctt gttggggatt ctgtgctggt tttttgttag cgataacaac
    13621 aacctgcgca gtgccaataa cgaagaaaaa cagtcattcc agcttagcga catccttgcc
    13681 gttttgcgta tcagcaccac ctggtattgc agcatggtga tttttggtgt cttcacaatc
    13741 tacgccattc tgagttactc caccaactat ctgaccgaaa tgtacggcat gtcgctggtg
    13801 gcagcgagct acatggggat tgtgatcaac aaaatcttcc gcgcgctgtg cggcccactt
    13861 ggaggcatta tcaccaccta cagtaaagtg aaatccccta cccgcgtgat tcaaatcctt
    13921 tccatcatcg gtttgctggc gttgacagcc ctgctcgtca cgaactctaa cccccaatcg
    13981 gtcgcgatgg ggattggcct gattttactg ctgggattca cctgttacgc ctcacgcggg
    14041 ctgtactggg cctgccccgg cgaagcgaga acaccgtctt acattatggg caccacggta
    14101 ggtatttgtt cggtgattgg attcctgccg gatgtcttcg tttacccgat tatcggccac
    14161 tggcaagaca ccctgcccgc tgcagaagcc taccgcaata tgtggctgat gggcatggcg
    14221 gcgctcggca tggtgattgt ctttaccttt ttgctgttcc aaaaaattcg tactgctgat
    14281 agcgcccccg caatggctag cagcaagtaa gccaggaaaa ttacaggaga aaaatcatgt
    14341 cgaaaaaata catcataggg attgatggcg gaagtcagag cacaaaagtg gtgatgtacg
    14401 atctggaagg taatgtggtt tgcgaaggta aaggcttatt acagccgatg cacacgcctg
    14461 atgccgatac tgcagaacat cctgacgacg atttatgggc atcattatgt tttgcaggtc
    14521 acgatttgat gagccagttt gccgggaata aagaagatat tgttggcatc ggcctgggct
    14581 ccatccgttg ctgccgtgcg ttattgaaag ccgacggcac gccagctgcg ccgttgatta
    14641 gctggcagga tgcacgcgtt acacgccctt acgaacatac gaatcctgac gtggcgtatg
    14701 taacctcttt ttcgggttat ctgacgcatc gcttaaccgg cgaatttaaa gataatatcg
    14761 ccaactattt tggtcagtgg ccggtggatt ataagacctg ggcatggagc gaagatgccg
    14821 cagtcatgga gaagtttaat atcccccgtc atatgctgtt tgatgtgcaa atgccaggca
    14881 ctgttcttgg gcatatcaca cctcaagccg cgctggcgac gcatttcccg gcaggactgc
    14941 cggttgtttg taccaccagt gataaaccgg tagaagctct gggagccgga ttactggatg
    15001 atgaaactgc ggtaatttct cttggcactt acatcgcatt gatgatgaac ggcaaagcct
    15061 tgccgaaaga tccggtggcg tactggccga ttatgtcttc tattccgcaa acattgctgt
    15121 atgaaggtta cggtattcgc aaaggcatgt ggacggtgag ctggctgcgc gacatgttag
    15181 gcgagtcgtt aattcaggat gccagggcgc aggatctttc accggaagat ttactcaaca
    15241 agaaagcctc tagtgtgcca cctggctgta atgggctgat gacggtgctg gactggctga
    15301 ccaatccgtg ggaaccgtac aaacgcggga ttatgatcgg ctttgattcc agcatggatt
    15361 acgcatggat atatcgttcg atattggaaa gcgtggcgct gacgctgaag aacaattacg
    15421 acaatatgtg taatgaaatg aatcactttg cgaaacatgt aatcattact ggcggcggtt
    15481 cgaacagcga tctgtttatg cagatttttg ccgacgtgtt caaccttccg gcacgccgta
    15541 acgccattaa cggttgtgca agcctggggg cagcgattaa tacaggggta ggtctggggc
    15601 tatacccgga ttacgcaacg gctgtcgata aaatggttcg cgtgaaagat atctttatac
    15661 cgattgagag caatgccaaa cgctacgacg cgatgaataa aggcattttc aaagacctaa
    15721 ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt gatgcatggg gaattgggga
    15781 attggggaat gcggattcga tccagagctg gtcgaatgcg tgaataaggt aggccgggcg
    15841 gaaaactgcc cggcctgaac atacctgaat ggttatcccc gctggcgcgg ggaacataag
    15901 ttcgacaact taaaaaatga taacaagtcc ggtttatccc cgctgatgca gggaacataa
    15961 taaaaacttt aactagttcg gtttatccct gtttttacaa ggaa
//
LOCUS       DAZPLM010000086        15961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPLM010000086 DAZPLM010000000
VERSION     DAZPLM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.086"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHVC7_002311"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHVC7_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR2116343.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHVC7_002312"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHVC7_002312"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR2116344.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHVC7_002313"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHVC7_002313"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR2116345.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHVC7_002314"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHVC7_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHVC7_002315"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHVC7_002315"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR2116346.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHVC7_002316"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHVC7_002316"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR2116347.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHVC7_002317"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHVC7_002317"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR2116348.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHVC7_002318"
     CDS             8229..8939
                     /locus_tag="ACHVC7_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR2116349.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHVC7_002319"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHVC7_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR2116350.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHVC7_002320"
     CDS             9636..10343
                     /locus_tag="ACHVC7_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR2116351.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHVC7_002321"
     CDS             10343..10891
                     /locus_tag="ACHVC7_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR2116352.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHVC7_002322"
     CDS             10901..12067
                     /locus_tag="ACHVC7_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2116353.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHVC7_002323"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHVC7_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHVC7_002324"
     CDS             13576..14229
                     /locus_tag="ACHVC7_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR2116354.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHVC7_002325"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHVC7_002325"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR2116355.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="ACHVC7_002326"
     CDS             complement(15612..>15961)
                     /locus_tag="ACHVC7_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR2116356.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3699 a         4344 c         4164 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAZPLM010000087        15767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPLM010000087 DAZPLM010000000
VERSION     DAZPLM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.087"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHVC7_002327"
     CDS             complement(468..1019)
                     /locus_tag="ACHVC7_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2116357.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1654..2001
                     /locus_tag="ACHVC7_002328"
                     /pseudo
     CDS             1654..2001
                     /locus_tag="ACHVC7_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            2054..2449
                     /locus_tag="ACHVC7_002329"
     CDS             2054..2449
                     /locus_tag="ACHVC7_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR2116358.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2490..3233
                     /gene="cmoA"
                     /locus_tag="ACHVC7_002330"
     CDS             2490..3233
                     /gene="cmoA"
                     /locus_tag="ACHVC7_002330"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR2116359.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3230..4201
                     /gene="cmoB"
                     /locus_tag="ACHVC7_002331"
     CDS             3230..4201
                     /gene="cmoB"
                     /locus_tag="ACHVC7_002331"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR2116360.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4366..6795)
                     /gene="torZ"
                     /locus_tag="ACHVC7_002332"
     CDS             complement(4366..6795)
                     /gene="torZ"
                     /locus_tag="ACHVC7_002332"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR2116361.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6820..7920)
                     /locus_tag="ACHVC7_002333"
     CDS             complement(6820..7920)
                     /locus_tag="ACHVC7_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR2116362.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8308..9054)
                     /gene="cutC"
                     /locus_tag="ACHVC7_002334"
     CDS             complement(8308..9054)
                     /gene="cutC"
                     /locus_tag="ACHVC7_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR2116363.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9068..9634)
                     /locus_tag="ACHVC7_002335"
     CDS             complement(9068..9634)
                     /locus_tag="ACHVC7_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2116364.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9850..11586
                     /gene="argS"
                     /locus_tag="ACHVC7_002336"
     CDS             9850..11586
                     /gene="argS"
                     /locus_tag="ACHVC7_002336"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR2116365.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(11628..11672)
                     /gene="yecV"
                     /locus_tag="ACHVC7_002337"
     CDS             complement(11628..11672)
                     /gene="yecV"
                     /locus_tag="ACHVC7_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR2116366.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            11763..12251
                     /locus_tag="ACHVC7_002338"
     CDS             11763..12251
                     /locus_tag="ACHVC7_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR2116367.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(12371..12763)
                     /gene="flhe"
                     /locus_tag="ACHVC7_002339"
     CDS             complement(12371..12763)
                     /gene="flhe"
                     /locus_tag="ACHVC7_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR2116368.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(12763..14841)
                     /gene="flhA"
                     /locus_tag="ACHVC7_002340"
     CDS             complement(12763..14841)
                     /gene="flhA"
                     /locus_tag="ACHVC7_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR2116369.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(14834..>15767)
                     /gene="flhB"
                     /locus_tag="ACHVC7_002341"
     CDS             complement(14834..>15767)
                     /gene="flhB"
                     /locus_tag="ACHVC7_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR2116370.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         3916 a         4111 c         3707 g         4033 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgatacagt ttaccctgaa aagagacttc acagattctt tatatatttc aataagttat
     1441 gctaaaaaga gactataccg atactgcata gaatttgtgt aggattgcat agatacttgg
     1501 aaaaacaccg ttaaattgca tagtcattca cctgatattg tcctgttttt acaggtagtt
     1561 gattcggatg ttctaggaat catcctcata gttgattcgg ttagttgggc ttgtaccaac
     1621 ccctaatcaa cctcatagtg tatttagacc ttgatgattt tagacagccg ccctgttcat
     1681 gcagcacgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg
     1741 gtacatgctg tactgacggc gacaaatccg ctgattcgtt ttatcggtag tgatgatatg
     1801 acgcaaaacc gggaattatt tcaggtctgg ttacagaaat tagcgcagtg gcatcagacc
     1861 actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga actggtacat
     1921 accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca
     1981 cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt
     2041 aaagggagtt tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa
     2101 gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg
     2161 ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat
     2221 cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat
     2281 ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct
     2341 gtttcgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct
     2401 ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg
     2461 ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc
     2521 ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata
     2581 tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt atgttagccg
     2641 agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga
     2701 cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact
     2761 ccccggcgat gattgaacgc tgcagtcgtc atattgacgc ctataaagcc cctacgccag
     2821 tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc
     2881 tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt
     2941 atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg
     3001 ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca
     3061 gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg
     3121 tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc
     3181 agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt
     3241 tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc
     3301 cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa
     3361 cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac
     3421 cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg
     3481 gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga
     3541 atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg
     3601 caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg
     3661 cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt
     3721 gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct
     3781 tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc
     3841 gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct
     3901 ggaaacgctg gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc
     3961 gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa
     4021 gtgtggtttt gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg
     4081 acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag
     4141 taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta
     4201 aaggtctggt attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc
     4261 taattcaatc tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt
     4321 acgtttgcat aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc
     4381 aggcttatca aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag
     4441 cgcagagtta ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac
     4501 gttcgcactg ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac
     4561 gcataccacg ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg
     4621 tttgttccag acacgcacca gatcgccatt cgcaatacca aatcgagcag catcttcggt
     4681 gtgaatagtg atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt
     4741 aagctgactg tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc
     4801 ggcggtaccc ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata
     4861 gccaaatttt tccagcgttt tggagtaaat ctcaattttg ccacttggcg tacccagcgc
     4921 atttttcacc ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct
     4981 gcggcgcatt tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac
     5041 gcgttgcgca cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc
     5101 atctttacct tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc
     5161 aaacacatca aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct tcatcggcac
     5221 aatatgctgg ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt
     5281 gatcggtaat acgatatctg cgtgtttagc agctgcggtc cagtagcatt cagaaacgac
     5341 gatcatctcc ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa
     5401 gttaccgcca cccgcccacc agatcatttt gatattcgga tacgtttgct ctttaccgtt
     5461 atgctgatat ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc
     5521 cctcattccg ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc
     5581 cggcagcacg ccgccaacac gtgtcgggtt gccaccattg gagtaatgat aagagaaacc
     5641 aaaaccgccg cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca
     5701 gtgtttctgt tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg
     5761 attcgccgcc atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc
     5821 cgccgcccag gccgcgctct tcggcgtatt atcgctctta cctctcagat actcttcaaa
     5881 ctgcggatac ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat
     5941 cagggtatgc gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca
     6001 ggtggcatta tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa tggcaatcac
     6061 tggtttgcca gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct
     6121 ccaggcaatt tttaaggtat tcagcgggtt catcccccac agcaccacaa cctggctgtt
     6181 ttccagaatc agcggccagg aggtctgctg ttcatacacc tctacagaac cgaccacatg
     6241 cggcatgatc acctgtgccg ccccggttga ataatcgccg ctatgcccgg aataaccgcc
     6301 cgccaggttc atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg
     6361 ccagccgtat gatccggcaa aaatagccga tggtccgtta gctttgcgaa tgcgatcgtg
     6421 ttgctcatga atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc
     6481 acgacctttc gtcggttgca gtggattatc gagatagctt tttctcacca tcggatgctg
     6541 aatacgcgtc gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat tcggtatggt
     6601 tttcgccagc gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc
     6661 tccccaacgt cccgcggtga ggattttacc gtccttctct tctgcccatg cggggagcgg
     6721 tgctgccgat gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg
     6781 tcttgttaat gtcataactt cctccctgat caacgaggat cactgtttct cggtaatatc
     6841 tttggcgttg tactggaaat accgcgttaa aatgtccagt tcgttttcgc tcatgctggt
     6901 tcgtgccccc attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc
     6961 aataggggca tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag
     7021 cggtttacgg tccgctaatc cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca
     7081 ttgattgccg tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc
     7141 ttcacttaat gtggctagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact
     7201 gccctgcatt tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac
     7261 ggtgagatcg gtaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg
     7321 ataaatatgt gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc
     7381 gttgtcatcc atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca
     7441 ggtttcgccg cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc
     7501 aatatccatg gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa
     7561 ttctttccat actgtttcgg ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc
     7621 tattttgccg ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa
     7681 ataatccatc cctgactttg gaatatggca atctgcacat tccgcacgta tccctttctg
     7741 gttcgaaaag tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga
     7801 aaggcaaaat gctgtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc
     7861 gccaccaacc acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgcat
     7921 agattcctct aaataaattc cagaacaccg aaatcattgt ataattaaat acacaacgaa
     7981 ttaaccgctt gcataactcg gcacaacact gcctgaaata atcgataaag aatatgattt
     8041 attacaatgt aatcattaat tgctaaggaa taacccagtt gctatttaga atatatattt
     8101 atcagtttta gcaatttaaa tcccataatc aatgtgaata taaaaaatat tattagcaca
     8161 acgctatgtt cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc
     8221 tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata
     8281 ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt
     8341 catttcagca accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc
     8401 tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc
     8461 ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac
     8521 tcctgctccg gccataatga ttggagcatc acgatgggca ataagttcca taatttttga
     8581 taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc
     8641 cgcaagatta ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt
     8701 cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc
     8761 gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac
     8821 atcctcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg
     8881 aattatcgga tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga
     8941 cggcgttaag cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg
     9001 ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc
     9061 tccttaatta cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact
     9121 taatagttgt tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac
     9181 cttttggtga actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca
     9241 atgccagcgc acgggcattc agcgtttctg gatcacccag cagaacgatt tcaatatgtt
     9301 cccaaccttc gtgtgggtaa cgtttttccc cgggccacgg tagctccaca atagaaaact
     9361 gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga
     9421 tcatattttc tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgttcagcag
     9481 tggcgttttg atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc
     9541 ccagacggag ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat
     9601 cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag
     9661 cgttaattta ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc
     9721 aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta
     9781 tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa
     9841 ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg
     9901 gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcatt cagcaaaagt tcagttcggc
     9961 gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatgac accgcgacaa
    10021 ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc
    10081 gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag
    10141 caggcgctgg cgtccgatcg tctcggtgtt tctacgccag aaaaacagac cattgtggtt
    10201 gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg ctctaccatt
    10261 attggtgacg cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac
    10321 cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag
    10381 caggaaaacg ccggtgatga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
    10441 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
    10501 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
    10561 acgcagaacc agatcaccta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
    10621 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
    10681 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
    10741 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
    10801 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
    10861 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
    10921 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
    10981 ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggccgatct gctggatgaa
    11041 gccctggaac gtgcacgccg tctggtggca gaaaagaacc cggatatgcc agctgacgag
    11101 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
    11161 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
    11221 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa ggcagaaatt
    11281 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
    11341 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
    11401 catgtgatgt gtgcttacct gtacgatctg gctggtctgt tctctggctt ctacgagcac
    11461 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    11521 ctgacagcga agacgctaaa gctgggtctg gatacgctgg gtattgagac agtagagcgt
    11581 atgtaatcga tttttcgtga gagtaaagcc tgatcagggt tacctgatca ggctttttta
    11641 ttgccatcta aatgtattct gaaaatggac atgccattgt tttctgactg ttggataaga
    11701 ggccagaagc gtaatatccg gccccaggga aacgataacg gttgaattta aggaataccg
    11761 cagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    11821 atacggttat agaaaacgac catgacattt cagccatcaa agattgttgg caaaaaaatt
    11881 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    11941 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    12001 gcacaggtgt aaaaaaacct gataccgaac aacttaactt gcttaaaaaa tcacagcgag
    12061 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gacgttcagg
    12121 atttaagtga cccttattct gaatcagaat ggctctcatg tatgatcata cagaccaata
    12181 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    12241 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaataat
    12301 tgcaacaaga catcgagcct ttttcactga gttattaaac atactcgcga gcgcgtaatt
    12361 tttttgtcct tcagcgataa ttcacaatca cttcattacg ttgtaccttt agcggtggaa
    12421 ttaaccgccc accgcctggc acttcccaga taaatcgcaa cggttctgct gccgctatac
    12481 cggaaaaggc cacggtggtt ccgctctgcc cctctaattc gacacaacga gattgcgaac
    12541 acaagcgaac ccgcagtcct gaaggtgtcg ggccgataag ctgataacgc cacgctacca
    12601 gcgtcatcaa tcctgaagca ggttgtcgcg tagaaagagg cgcagacgac atcgactcac
    12661 cgcgatgatt tagcgtaatt cctacactac ttgcctgcca catcccctcc ccggcggctt
    12721 gcaccagcag cggaaacaat aatattgcta ataaggttct cattatttgc cgccaattgt
    12781 cgccgtcatg cggatatgtc ggttatcaga cagttccaga ttcgacagga ccactaactg
    12841 cggcaagctg cggcgcagga agcgagacaa taatggtcgc agcgcgtggt tcaccaacaa
    12901 tactggcggc gcgcccagca tctcctgacg ggatagcgct tcctgagttt gcgccagtaa
    12961 acgatccgcc agccctggct ccagtcctcc cccgccctgc agcgcctgta gtaacaaacg
    13021 ttccagcggt gtatcgaggc caataacatg gacttcatct ttgccaggaa accactgctg
    13081 ggtaatcgcc cgtcccaacg ccacgcgcac gacggcggtt aattcatgtg gatcgctttg
    13141 gatgggcgca tgttccgcca gcgtttcgag aatggtgcgc atatcgcgaa tcggtacttt
    13201 ttcatcgagg agattttgca gcactttatg cagtgtggtg agcgtgacga cgccaggaac
    13261 gagatcttcc gtcagctttg gcatctcctg ggcgacgcga tccaacagct gttgcgcctc
    13321 ctgacgacca aacagctctg cggcatgctg gctaatgagg tggttaagat gcgttgctac
    13381 caccgtgctg gcctcaacca ctgtgtaccc ctgaatctgc gcctgttctt ttagcgcact
    13441 ttcaatccag atagcattca ggccaaatgc cggatcgacg gtcgcctcac caggtaacgt
    13501 cccggcagcg gttccagggt taatcgccag ccagcgcccc ggataagcat caccactgcc
    13561 aatctccacg cctttcatca aaatgcgata gcgggcaggt tgcagatcca tattgtcgcg
    13621 aatgtgcacc actggcggca gaaatcccat ctcctgggca aatttcttgc ggatactgcg
    13681 tatacggccc aacaactcac catcctgctg gaaatcgacc atcgggatca gtcgataacc
    13741 cacttccatt cccagagaat cttccagttg tacatcgttc cacgtcgctt cgacaacggt
    13801 attattctct gccattttta ccggtttggg ttcggcaggc gctttttgtt cgcgtccgcg
    13861 tatccaccag gccagcccga gcaatccggc agtgaacagc aaaaatacca ggttcggcat
    13921 tccaggcacc aggccgagta aaccgagcac ggcggcgctt aacaacataa cgcttgggtt
    13981 actgaaaagc tgattcacca tctgctcgcc aacatcctga tcggtgctga cacgcgtaac
    14041 gatgaccccc gcggcggtag aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc
    14101 aatggtcaat agcgtataac tttccgccgc gtgtcccatg ctcatgccat gttgcagcac
    14161 gccgaccagc aacccgccga caacgttaat gaccatgatg aggatcccgg cgatggcatc
    14221 gccgcgaaca aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac
    14281 ttcggagcgg cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc
    14341 aatcgccatc tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgcgat
    14401 acgcccggca cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac
    14461 gataccgata gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc
    14521 tgccgccgcc gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag
    14581 tgccagacgc aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt
    14641 gcgctgggta aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa
    14701 caacaggtcg agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat
    14761 caaaatcggt ccggcaagga tctgccattg tgtcgatttc aggtttgcgg gcaggcgcag
    14821 catcgcggcc agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca
    14881 ccggaagatg agtaggttgt acagggcgct gtccaccagc cagacgccag cgtttcagtt
    14941 gccagaccca ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac
    15001 caatctccgc atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt
    15061 tattttcagc gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca
    15121 ctttcggtgc gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt
    15181 tattgacaat gacatcagct ttcggcacat cggccatcat ccgacgccgt gcagcagctc
    15241 gctgcatctg acggatccgc cctttaacat gagggtcacc ttcgctttgt ttgaactcat
    15301 cacgaatatc ctgccgcgac atacgcagct ttttcaggtg gctgaagatt tggaaaaaga
    15361 cgtcaaatcc caccattgga atgacaccaa gcaccaccag cagtgcgcat agcccaacca
    15421 gatccatcgc attacccatg gcggtagtcg gagactcggc cattaagcgc atcatctgcg
    15481 gccagtgatg ccagagataa aaccccgtca cgctgccaac caggatggtt ttcaaaattg
    15541 ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg tttaatgccc ggtagcgggt
    15601 tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa taccagcccc cccagcatga
    15661 ccggagaaat gagcgccacc agcaccacgc cgctaatcag cggcagcagc gccagcatgg
    15721 cttctctgat cagcagaata atctgcccga ggatcagatt cgggtca
//
LOCUS       DAZPLM010000088        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPLM010000088 DAZPLM010000000
VERSION     DAZPLM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.088"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHVC7_002342"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHVC7_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR2116371.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHVC7_002343"
     CDS             567..650
                     /locus_tag="ACHVC7_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116372.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHVC7_002344"
     CDS             750..1571
                     /locus_tag="ACHVC7_002344"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR2116373.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHVC7_002345"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHVC7_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR2116374.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHVC7_002346"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHVC7_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR2116375.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHVC7_002347"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHVC7_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR2116376.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHVC7_002348"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHVC7_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR2116377.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHVC7_002349"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHVC7_002349"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR2116378.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHVC7_002350"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHVC7_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR2116379.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHVC7_002351"
     CDS             8347..9729
                     /locus_tag="ACHVC7_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2116380.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHVC7_002352"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHVC7_002352"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR2116381.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHVC7_002353"
     CDS             complement(10485..10820)
                     /locus_tag="ACHVC7_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR2116382.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHVC7_002354"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHVC7_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR2116383.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHVC7_002355"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHVC7_002355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR2116384.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHVC7_002356"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHVC7_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR2116385.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHVC7_002357"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHVC7_002357"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR2116386.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEQGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3793 g         3886 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc ctgctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPLM010000089        15485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPLM010000089 DAZPLM010000000
VERSION     DAZPLM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.089"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..192)
                     /locus_tag="ACHVC7_002358"
     CDS             complement(<1..192)
                     /locus_tag="ACHVC7_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116387.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDD"
     gene            complement(435..1484)
                     /gene="yahK"
                     /locus_tag="ACHVC7_002359"
     CDS             complement(435..1484)
                     /gene="yahK"
                     /locus_tag="ACHVC7_002359"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR2116388.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1571..1645)
                     /locus_tag="ACHVC7_002360"
                     /pseudo
     CDS             complement(1571..1645)
                     /locus_tag="ACHVC7_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1861..3243)
                     /locus_tag="ACHVC7_002361"
     CDS             complement(1861..3243)
                     /locus_tag="ACHVC7_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR2116389.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(3253..4203)
                     /locus_tag="ACHVC7_002362"
     CDS             complement(3253..4203)
                     /locus_tag="ACHVC7_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR2116390.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(4346..5764)
                     /locus_tag="ACHVC7_002363"
     CDS             complement(4346..5764)
                     /locus_tag="ACHVC7_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR2116391.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5764..7311)
                     /gene="fdrA"
                     /locus_tag="ACHVC7_002364"
     CDS             complement(5764..7311)
                     /gene="fdrA"
                     /locus_tag="ACHVC7_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR2116392.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7301..8164)
                     /locus_tag="ACHVC7_002365"
     CDS             complement(7301..8164)
                     /locus_tag="ACHVC7_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR2116393.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8204..8809)
                     /locus_tag="ACHVC7_002366"
     CDS             complement(8204..8809)
                     /locus_tag="ACHVC7_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR2116394.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9067..9564
                     /locus_tag="ACHVC7_002367"
     CDS             9067..9564
                     /locus_tag="ACHVC7_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR2116395.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9656..10588
                     /locus_tag="ACHVC7_002368"
     CDS             9656..10588
                     /locus_tag="ACHVC7_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2116396.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10630..11718)
                     /locus_tag="ACHVC7_002369"
     CDS             complement(10630..11718)
                     /locus_tag="ACHVC7_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR2116397.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(11860..>15485)
                     /gene="ehaA"
                     /locus_tag="ACHVC7_002370"
     CDS             complement(11860..>15485)
                     /gene="ehaA"
                     /locus_tag="ACHVC7_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR2116398.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3541 a         4223 c         3699 g         4022 t
ORIGIN      
        1 gtcatcgact aaatcatcac gacaatcata aggataatca taatattgtt taatcaaaac
       61 cttgaatata tcgacacagg aaaccaggga gcttttgata tcctctggta actgattgtt
      121 tttaaccgta ttaagtatgc tttgttgcgt atatggcatc aaattattgt gcggagcttt
      181 taatgatatc atcgcgatct ctcttaaata aaaataaacg tcgcagataa taaagcgctg
      241 attaaaccag gcactatcag aaatcgctca taactaacgt gagaagtatt gtgtactgga
      301 gggagttaat ttaacaattt gaatttacgt tataggtttt actgctactt tgatattatt
      361 tcgctctcct taactaaaaa gttaagtgga gagcgaagtt aatatgttaa accacagcgt
      421 atttattaat tttttcaatc tgttagtgtg cgattatcga taacaaaacg atatttcaca
      481 tcaccacgca gcattcgctc ataggcttca ttaatttgat cggcccgaat catctctata
      541 tcagccacga tgccatgttc ggcgcaaaaa tcgagcatct cctgagtttc tggaatgccg
      601 ccaatcatag aatcggctat cgcacggcgt ttcatgatca ggttgaaaac ttccggcgat
      661 ttatgcggtg tcgcaggcgc accaaccagc gtcatggtgc catcacgctt cagcaaggtg
      721 gtaaaatcgt cgagattatg tggcgcagct actgtattca aaatgaaatc gaaactcttc
      781 agatgagccg ccatctcatc ggcattgcgt gagttaacaa cttcatcggc ccccagggct
      841 ttttccgctt cgcgttttgc ctcagaagtg gtaaatgcca ccacatgtgc ccccatcgcg
      901 tgggccagct taatccccat atgtcccaga ccgccgatgc cgaccacgcc cactttttta
      961 cccggcccgg cctgccagtg acgtagcggc gagtacgtgg tgatccctgc acacaacaaa
     1021 ggagccaccg ccgccagctg ctcttgcggg tgacgaatac gcagaacata tcgctcatga
     1081 acgacgatct gttgtgagta gccgcccaga gtatggcccg gttcgtccgg cgtcggcgag
     1141 ttataggtgc cgatcatgtg atcacagtag ttttccagcc cgtcctcaca ctcttcgcaa
     1201 tgtttacaac tgtcgacaat gcagccgaca ccgaccagat cgcccggcgc atgtttttct
     1261 acctgatcac caacggctac cacacgcccc acaatttcat gacccggtac gcaggggtaa
     1321 accgtccccg cccactcgga acggacctgg tggagatcgg aatggcagac gccacagtaa
     1381 gcgatttcga ttttgacatc attcggtccc ggttcacgcc gtgtgatatc catcggttca
     1441 agtggttgtt tagcggaata tgcaccaaca gctttgatct tcattgtgtt tactcctgat
     1501 tagctatgtg tatttggcga cgcctgatat gtcatcgcgg caaaacgcgt ccattgaata
     1561 tagccaatat ttaaaccacc cgatccagcc ccaacatgcc gcgaacctga atgaaacaga
     1621 gcagcaacgt tccccataag gcgagcgcct ataagtgtga tatttgctat ccgtaaaaga
     1681 ctaaaatcgg caaaactgat gatcttttca cgtagacaaa tagttatcta cacaacgtca
     1741 ataagggcat attcctcaaa gataatgtcc atttctgccg acactatcat attgaggtcg
     1801 ccccactccc tggggcttaa atctcttaca attcaccgtt gcaggcgttt caaatgcgca
     1861 tcaacctgcc acactccccc acaccagttg ccctttatgg aacgttgcgg ttctcggcga
     1921 gatacgcgcc accgcctcgg cggaacagga ggcgtccacc agcacaaagc tggcatcatc
     1981 ctgcgctttt ggccatacac gctcgccttt ttcattcagc ggcaatacat cgccagtggc
     2041 tagaaacagc gaacgggaga ggttctgttc gttaggacga atatagagct gcgcgtagag
     2101 attggctttt tccagcatgt cgcccagacc ataaggcgac cagtggtcga taacgctgtc
     2161 agtgccagtc atcactttta cgcctttgtc gtgcaactgt ttgagcggca tatgcagcgt
     2221 gccaatcggc accgtcgagg cgatagaaat ttgctgcgcc gccatccgat tcgccagcgc
     2281 atcgacctgt tgttcgttga gcgtagccag cgcaaaggcg tggctgatgg tcagcttacc
     2341 tttcagttgc ggcgttttct ctaccgtttc aaccatataa ttgatggctg ccacgcccga
     2401 cggagtggtt tcgtgcaggt gaatatcgac acctttgtcg tagtccagcg caatctgaaa
     2461 catggtgtcg agggattttt ccatcgcgcc atcgacactg gtcgggtcca gcccgccgac
     2521 gtaatgcgca cccgcctgca tcgcttcgcg cattaagggt tccgatttcg acagcagcaa
     2581 accgtgctgc gggaaggcga cgatttcaca ctcgaagccc gcctgacgcc gcgccagcac
     2641 cgcctgcaaa ttttgcagat tttttaggcc ggaaaccggt tcgatattgc agtggctgcg
     2701 ggcaatggtg gtgcctttcg actgcaatag atcgatcagt ttttccgccc gctcctgcgt
     2761 atacggttgt aactccggca gcattttctg cttgagtttg atcatgtcct ggatagtggt
     2821 gcctgccgga cgattgagcg agcgccacgg cccgccgtag aaggttttgt cgagatgaat
     2881 atgcatgtcg cgagtggtgg gtagcatcaa cttaccgcca gcgtcatagt gcggcagcgt
     2941 ggcgtccgga tgctgcttgt tctcgcgcag ggcgacaatt ttgccgtcct gaatctccac
     3001 ggtctggcgc gccgtgcggg tctgcaccgc cacaccgttt tcgtagtcaa aaccggtttc
     3061 cagcagcacg ttatcgagat agtaatgcgg gtcagtgatt ttcatggtgt tgttcgcttc
     3121 gcagtttagg gtgccagcta ccgccgcatg ggcgagcggc acataggtac caaacagcgc
     3181 ggcggcggtg accatcttac cgctctggct cagaaattca cggcggctat tgctttcttt
     3241 catcctacgt ccttacgttt taataatatg ggtgcccgtt tcaccgcgca gcgccgcagg
     3301 caggcattcc ggcgtggtga taatcacttc tttgccgccc tgctccagga acgtcaggct
     3361 ggcgatgatt tttggcaaca tgctgccggg agaaaaatgc ccttcctgca tatagcgagt
     3421 catggtggca atatccaccc gatccagcgc ctgctgctgc ggtttaccaa agtggataca
     3481 gactttttcg acgtcggtgg tgatcaccag aatgtcggcg tgaatttcac gggccagcag
     3541 cgcggtagag agatctttgt caataaccgc gtccacgctt tggtaatccc ccgcttcagt
     3601 acgcactacc ggaattccac cgccgcccgc gccaattacg acaaaacctt gttggatcag
     3661 cgccttaatg gcgggtgctt cgacaatacg tttcggttcc ggcgaggcga ccacgcgacg
     3721 atagccccgt ccggcatctt caacaaaaca ccagtcaggg tttgcctttt gtagtttgtc
     3781 acgctggctt tcactgaaga atgcaccaat gggcttggtg ggatgggcaa aacctggatc
     3841 gtttttatcc acttccacct gagttaccac ggtgacggct tttttctcgc cgtgacgcgc
     3901 cagtcggtta ttcagcgcct gttggatcag atagccgatg ccgccctgcg tatctgccac
     3961 acagttcgcc agcggcgtta agggcagccc ttcgcgctcg tgggcaatct ccgcacggcg
     4021 taaatccagc ccgacctgcg gcccgttacc gtgggtcagc acaatgtcgt aatcggaagc
     4081 cagcatttcc agcaccgtat cggccacggc tttcaccgcc tccgcctgat gctcaatcga
     4141 ctggctggcg ttatctttga taatgctgtt gccaccaatg gcaacgacca caagctcttt
     4201 catgttgtta tgtccgctac agagggtgat gaggttatga ccaacgccag actgcctgat
     4261 gcgctacgct tatcaggcct acaattctcg ggccatccgt aggccggata aggcgttcac
     4321 gccgcatccg gcataaacat gtaagtcaga cgccgtaacg ttcgcaccag ccgagaatgg
     4381 ctttctcgaa gcaagcgaat ggtggatgca caatgcccgc gccaatcatg ccgacgcccg
     4441 catctttatg ggcgatggcg gtgttgatca ccggcagaat gccgctgcta cccacgcggg
     4501 tgatgtcgat tgccgacggc acgcccataa aaccgagcag cggaatggtg acgttggggt
     4561 tttcaccgag agtgatttca cgcatctgac gggagaaatc gatagcctct tccaccgtgc
     4621 cgcccaccag cgcgacgatt gcaggcgcgg tcgccatagc aaatccacca ataccgtagg
     4681 tttcggtgat ggcgctgtcg ccgatatcca gccccgaatc ttccggctta taaccggcaa
     4741 acatcgggcc gatcacctgc tgcgccgggc cggtaaacca ttgccccggc aggccgctga
     4801 cccgcaggcc gaactcgacg ccgttacgcg ccatggtggt gaccacggtg ctgtattcga
     4861 tgccgtgcgc cgcatccatc gccgctttac acatcgccat ccacgtcggg ccggagaagt
     4921 agtcgctgct ggcgacaaac tcgaacactt cgcgctgttg ctcgacggaa tagcctgcct
     4981 gaatgatccc cggcgtcagt gcctgaatca gcagcgtcgt cccggcgtta ttgcggttat
     5041 ggcactcatc gcccatatgc agcgcctgcg ccagcattaa gcgcagatcg atctcaccga
     5101 taattttcat cgcgtcgcgc agcattggcc cctgcacatc gcgcatccag ttgaggcggt
     5161 cgatcacgct ctgatcgttg gcacccatac gcaaaatctt cgccatctgc tcgctcatat
     5221 tggtgtaagc gatgttgccg taggttttgt ttttcacgat gtgcataaac atcgaggccg
     5281 aggtaacgcc cgccatcgat cccacacagt cgtgttcgtg gcacggcgag aaggtgatct
     5341 ccccggaggc cgccagttca gccgcctcgt cgagatcttt cgccagtcct tcgaacacca
     5401 gcgctccggt gaccgcgcct ttcatcgcgc cgcacatttt ttcccaggtg atcggcggcc
     5461 cggcgtgaag aatggttttc ggcgtcatgc ccggcaccac gttaatcgcc tgatcaaaac
     5521 caatcagcac cggatgcgac tggataatgc gctccagcgc ctgctggtta gcggcggcga
     5581 ttttgtctgc cagcggcgaa tcggcaatgt tatccagcgc ctgcaccacc tgcatattgc
     5641 cctgccccgg cggcgtccag tcgagttgag tcacttctac atgctgcttt ttcaggtcat
     5701 cgctaaacat ggcgatgccg acgttaataa cgttcaatgg ttggctaaac agtgactggc
     5761 tcatcaggct tcctcccctt tgcagataaa ttcacgcgcc agcaatccgg tattggtgct
     5821 gctgctcgcc agaatcactc cggcatcaag cagcatctgg ctttgttgtt ctaacgatgg
     5881 cgtatcaaga tcggtaccca gcacataggc gagaatgatc aactcgcgtc cttcggcagc
     5941 ggcgatcgct ttcgcttctt tgatcgcctc gatggtggag ccgaccggat cttcatgcga
     6001 tccaaatccg agcacaaaat ccatcacgat caccgccact tctggatcgc gcgcctcttc
     6061 gatcaagcga ctgatgcggt tggtggggtc aatcattgga tgcggcttgc cgttcgtgaa
     6121 gtcgtcatcg ccaaagtcga ggaaggtgtg tttgatgctg cggttgatat cttgcaggcg
     6181 gaattccgga tcgggctgaa tgttgctgta gacatcgcca tgtttttcca tcaccgcgaa
     6241 catggtttcg tcgcacagcg tgccgccgca gaacaggcca cgaatgtatt tctgctgcgg
     6301 ttgcagacgc gcacgcacat cagcaatcaa cggctggtta agcgtatgca ggtcgagatt
     6361 ttcctgtttc acgccggaga gcatcaccgc ttttagcgcc gcctctttgc tgccgcgggc
     6421 aaactgtagc ccctgctcat ccaccggcgt ttcaacgcga ccgaggaagc agaccaccac
     6481 cggcttgcgg caggcacgtg cgcgttccag cactttgcgg gccaccgcag gcgcgggagg
     6541 ttttgaaatc agtgcgataa tttcagtttg cggatcgttt tccagcatcc cgatggcgtc
     6601 gagcatcatc aggccgccga ttttctcgct caggtcgcgc ccgccggtgc caatcagttg
     6661 tgaaacaccg ccaccaaatt catgaatgcg gacgctcagc tcctggctgc cggtgccgga
     6721 tgcgccaaca ataccgatat tgccgcgacg cacggcgtta ccaaaacaga gcgccgcgcc
     6781 gttaataatc gccgtgccgc agtccggccc catcatcagc agcccttttt cgtgggccaa
     6841 ctgcttgagc gccagttcat cttcaactga gacgttatcg gaaaacagca tcacgttgag
     6901 atcgttttgc agcgcctgac gcgcttcgcg agcggcaaac agaccgttga ccgaaatcac
     6961 cgccaggtta ctttccggga tatgcttttt ggcgctggca atagtggcgt aacgcgcctc
     7021 gtgcgagccg ctttgcgctt tggtgttgaa cagttcttca atctccacca gcaactgctc
     7081 gttgtccgca cccgatttac cattgatgac aatcatcagg tcgccgtttt tcgcctgttc
     7141 cagcgtcggc gtcagcagtc ctaaattttt cagcacacct ttgttcattt cggtcgccat
     7201 cgccacaaat gcctgctcga cgccgtcgag tttatttgca cgcgtggaga tagacatcag
     7261 cgagacagaa tcaaaatagg tattcggttt aatgactatt ttaactgaca taattccctc
     7321 cgtaggtttg gctcagcgca caaccatcgg ccatgccata cagcatgtcg cagccggaac
     7381 tggagccaat atgtttaatt ttttctattg cctgagttga attcccaggg acgccataaa
     7441 taatgttgtg aataaagcga tgaatatttt cccggcagcg ttgttgtaat gcggcttcca
     7501 gcgttatggc acttaataac gtggtgttat ttctgccgcg tagcagaccg cgataaaatt
     7561 cctctttgta tttttctgcc ggatgcccgg gaataaataa aataagcgcc agacctgtta
     7621 aataatcatc ggctgatggc gtaaggccaa tccctaaccc aatcatactg gcgaccgctg
     7681 cggcgatatt ttgcttatcg tttactgtct gaataactgc ccgtcgttta atatgtaatt
     7741 ctttatttaa cgcctgataa aacggattat cgccgcgata attaaacagg gtttcatttt
     7801 cccccaggtg ctgatgaata atgtcgtgcc actgtagcca ggcgataagg tgaaaattca
     7861 cagcggtcat ttgcaaggtt ggggacagcc aacgccgaca acgactcatc tctatatgaa
     7921 gatgttgacc aaccgtaata ccttgatcgt caaactgaac cttatcacca tgccggaaca
     7981 gatcatcaaa gtgagtgagt gccaaccgac aactgtttgg cgcattgtcg tactcttcac
     8041 aaagcagcgt cagcaactgc tggcgagccg ggagtgcgat atttaccgct ctggcaaaga
     8101 cctgttcaac ctgtccttgc ccccgctgcg ccagaaaatc cgcatccgca gttagcgccc
     8161 acacacaccg tttttctggt gtgttgatag ccagcctccc ggtttatgca cctgcggcaa
     8221 tcagtaactg cgcaatctct tcaaagcccc gttcccgcgc cagttccagt ggcgttttgc
     8281 catatttatc ggtcagatgc gggctggcac cgtgttccag caataactgc acaatcgcct
     8341 gctgtttaat accaccatca ttaagcacaa tcgcttccag tagcggcgtc cagcctacat
     8401 ggttggtctg gttaacgtta atctccgtat gcgccaaaag ctcttttaca atacttaaat
     8461 ggcctttttc acaggcaggc gtcaggccga caccgccaaa acgggttacg caattaagat
     8521 cgggtttagc cggtaaaata attcgtagta gcgttaaatc atcgttcaga cagctaatta
     8581 aaaaaggatt taaacaggta tgatcttgct tattaatatc cgctccggca tcaattaatg
     8641 cctgaacgca agcatattgc tgatataaac ttgccagcgt aattgccgtt ttcccctgac
     8701 gatcgcaggt attaatatcg acacccagcg caaggcaggt ttttacttta tcgatatcac
     8761 cctgttgtgc agccaataaa tagtcggcag gtagattttt tatactcatt cacattcccc
     8821 tggtttttgt ggctgttttc gatgaattga gaatagcaac gataatctga taaaagaacc
     8881 cgcttaaatt taattcgccg tatctcgcct ttttgttgaa taatattcaa caatcttttc
     8941 gcagtgtgcg gtagtgcaat atttgtttct ttgacccgat accctttcac tcaatcaggt
     9001 tttatttctc tatagtctgc acatgtaaga gtagctttcg tccatgtcta accagagggg
     9061 aacaagatga acggactcac ggcaacgggt gtcaccgtcg gtatttgtgc agggctttgg
     9121 cagttagtgt cttcacatgt cgggctctct caaggatggg aattattagg aaccattggc
     9181 ttcgtcgcct tttgcagttt ttatgccgca ggcggcggta aatcggggtt tatcagaagt
     9241 ctggcggtta attattccgg gatggtgtgg gcgtttttcg ccgcgctgac tgctggttgg
     9301 ctggcctctg taagtggact gtccgcgttc tgggcgagcg taattactac cgtacccttc
     9361 tcggcggtag tggtctggca agggcgtttc tggttactgt cctttattcc cggtggcttt
     9421 ctcggcatga cgctgttttt tgccagcggg atgaactgga cggtgacgtt actgggtttt
     9481 ctggcgggta actgcgtggg gataatttca gaatatggtg gacaaaaatt aagcgaagcg
     9541 acgactaaac gcgacgggta ttaaaagtta ttcaaaaatg attatctatc tttaaccgat
     9601 acctctacag tcatagctgg agaaattcat agtagataat ggcgagaatg gtttaatgaa
     9661 ctcaattttt accgaagaga atctgttggc atttactacc gctgcgcgtt ttggcagctt
     9721 cagtaaagcc gcagaagagt tgggtttaac cacttccgcc attagctaca ccatcaagcg
     9781 tatggagacg gggctggatg tggtgctgtt cactcgcagt acccgcagca ttgagttaac
     9841 ggagtccggg cgttattttt tccgcaaagc taccgacctg ctgaatgatt tttatgccat
     9901 caaacgcagt atcgatacta tttcgcaggg catagaagcg cgtgtgcgca tttgtattaa
     9961 tcagctgctt tatacgccca aacacaccgc acgcttgtta caggtgctga aaaagcagtt
    10021 tcctacctgc cagattaccg tcaccaccga agtgtataac ggcgtctggg atgcgattat
    10081 taataatcag gccaatatcg ccatcggcgc acccgataca ctactggacg gcggcggtat
    10141 tgattacact gaaattggcg cgattcgctg ggcttttgct atcgccccgg atcacccgtt
    10201 agcctttgtg ccggaaccca tcgccgaaag ccaactgcgt ctctacccta atattatggt
    10261 ggaggacacc gcgcatacga ttaataaaaa agtgggctgg ttgctgcacg ggcaggagtc
    10321 aattctggtg ccagatttca acaccaaatg tcagtgccag attttaggtg aaggaattgg
    10381 ttttttgccg gattacatgg tccgtgaggc gatgacgcaa tccctgctgg ttacccgaca
    10441 aatccataat ccgcgccagg attcgcgcat gttactggcg actcagcatt ctgctacggg
    10501 tcaggtgacg cagtggatta aaaaacaatt tgcgccaaat ggcatattaa cggggatcta
    10561 tcaggattta ctgcatcggg agaattaatc gctgccaacc tcccgatgcg ctgtgaatag
    10621 cggtttacat caaccacctg ctttcattac ccattcagag ataaatttat tacccggtac
    10681 tggcggagag tacaaatagc cctgcaaaaa gtgaacgcct ttaccgatca ttaaatccgc
    10741 ctgctcctgg gtttctaccc cttccgccac gatactcaga ccaggcttac gcgctagttc
    10801 gacaatattg tccacaatat gaccggagat ttcgtcgaca ctcgccattt gcacaaatga
    10861 cttatcgatc ttaataaaat cgaccgggaa cgcctgcaag taacgatagg tcgcataacc
    10921 cgtaccaaag tcatccagcg caaaggtaat gttgtgctga tgaaggctgt caaatatcgc
    10981 tctggcttct ggcgttaccg gaatagggtt acgttccgtt agctcgagaa ccagcttgat
    11041 tttatcgtta cctaatttct taaccaggtt cagacactct ttttcaaatc ccgctgccaa
    11101 aaaacaaccc gccgagacgt tgatgccaat atggaaattg tccggcagca aatgttttac
    11161 cggcatcaga atatccgcag tctgtttcat cagttggcgg gtcattatga caataagacc
    11221 ggatgactcc gccagaggaa taaactgatc cggtgggata attcccgttt gtggatgttc
    11281 ccagcggaca agtacctcac agcccgtcag taccccagtc tgcgcgcaga aaaccggttg
    11341 gatccacggt ttgaattcgt ggttttccaa cgccagactg atggcttcag gcgtgacgat
    11401 atggtgacaa cggttatcag ggatataaaa attattttta tttcccgtgc cactgatgac
    11461 ttgcggatga tattgaaaaa agatatcccg ccagaaggta atatcactct ggatgcctag
    11521 tttttcatac agctgcttct tttgatgtga aattgtcttt gcactgcgat ttctgtactg
    11581 tgagatttct gtaaccgaca ttccctccat tagcagcttg agtaaaagcc gttctttttc
    11641 ggacacgata ccaggcaacg tcaaattcac cgttgccccg aactcttcca aaaaaacact
    11701 caaatcatgc gaagccattg aacacacctt gattaaatct tgtgcgccag ctaaatctgc
    11761 gagtcttcac tctgactgat aaagctggct gcaagaaaat gacacagaga gccgaattat
    11821 ctcccgttcg gctctctctc cttttactca ttattcagat cagaattgcc acttaatgcc
    11881 aaccattgca gaggtgtcat tatatcccct atcggcaacc tgaacgccca cattccccca
    11941 gacattcagg ttggcattta gctgtccttc caccccggtt tttatctcag caatatttcg
    12001 ggctccatcc tgagtgacgg acacgccatc catactggtg ctgaaatcct tactgttatg
    12061 cagccagttc acttctacaa acggctggaa ctcgcgggat ttaccgtcat ccattttatg
    12121 gtggctcttc agccaggttt ttacgccaag tcgggtttga acattgccat caccgttgct
    12181 atgaacgagg gttccgttgc tttcgcggtg cttatcggct ttgactccca tccaggtaac
    12241 ctgtgcttgc ggctgaacat accattcatt acgcgttccc tggctgccat taaattcagc
    12301 taatttgtgt ttgtatccag cttccagtga agcggtaaat ccttttgatt tataggattc
    12361 actttgtaag tcatcccctt tcactgtgtt atcaaaccag ctgtactgcg cccaactgtc
    12421 gagatacgcg ccattacgcg actcgtcatc ggcataccag gtggcataga ggccggtgct
    12481 atatccgttc acactcgctt ttgcacgata accggttcgc gaggaaatgg ttttgctgtc
    12541 gctgttgcca tatcccgcca tgaccccaac atgccagctg tcgctgccgt tttggctcca
    12601 ctgcgcgaca tcgcctccca gttgcagaac atagcgattg ctttgggttt tcagctggcc
    12661 gctgccatca cgccatttat tatgaccacc ttcatggcgc atccacatag tggtttgttt
    12721 ctgctcaccc gtcaccatgt cggtatagta cgtattaccc agacgctcat gcagacgcgt
    12781 ggtgaacatt gtattcgctg ctgcaaggtt cgcaatgtag ctacccgcct ccggtcgcag
    12841 gtcattatcc acattcagat ccggacccgg cgtaggtgtc gggttagggt tcggctctgg
    12901 attcgggttt ggctccggat tcggcatcgg gtctggctcc ggctgcagtt caggagaatc
    12961 actgccgctg gtcagatacc agttaccact atttgcccct tgtccacgcg cgagagtgta
    13021 gtcatacgcc cccgcaacga tacgaccagc ctgaacaaat tcgccctcag acttaccgtc
    13081 tacatggata agttcgatac cgttaagggt tttcgcacct gtaccgccag cgttattcac
    13141 cgtaacggca gtcgtgccgc tagtatcgcc ctcgacaacc aatttatcgg ttactgagtc
    13201 atcgccattc agtaccgtat tcattactag ttgaccgtta ttaccgtggt agttcccctt
    13261 caccactaac acattgccag cttgctgtcc tgacattccg gtcatcagcg taccgttgtt
    13321 ggtcagattg ccgcccaggg tgtaattcac agacgcagcc gctctttttt cagcgctatt
    13381 attcaacgcc agggtaccgt tgttctcaac gtttgcgcta cccaatccct gcgcatgact
    13441 taccaggaca ttagccgaat tagcaatgtt cacattcgct gtcaggttgc tgttatcgcc
    13501 cgcgatgtcc agcgtgccgc cctgaatatt cagctctccg ctccccgtta acgaaccggt
    13561 tgaagtgccc ccgttggtaa ccgtcagact gccgccattt aagctcagca gtgaatcggc
    13621 gctgccattg agcgtgccca cggtctggct gtgaccattc atatccagtt cagtctctgc
    13681 cgccagacgc agttcatggg tattacccaa cacgttatcg ttattcaaca aaagcgtccc
    13741 actgcgcaga tcggtaacac cggtatagtc gttgtctttg ttagaaagcg atacggtctg
    13801 acccttctgg ctgctgaatg ccaggtcacc actgccggta atctttgcgc tgagatcggc
    13861 ggcattctcg cttttaccgt tagagctcag caccagagca tcgctgtcgg tagcgtgtaa
    13921 atcaagctgg gtcaggccgt aaccgatata cagaccgtca ttgtttaccc cactcgtcag
    13981 acgatagtcg taagtgccga cggccgcttt ttgcccattc tgctgaatat cacgttgggc
    14041 actgctggag aggatttgcc catttttatc ctgcaattgc agattgcccg catcgcccag
    14101 aacttccgca ccttgcgcat caaccagctt aatggcgctg ttaccatcat cgacttcagt
    14161 gagcgataac gcagagtcaa tatcacgaga tactgagcgg acaacgtcac tgtcagaaac
    14221 ctgaatcaca ccctcgccgc gcaaatccag cgttttacta acgttgaccg tcccttctgt
    14281 catctgtgca cctgctgtca gggcaccaaa atcaacggtt ccgccggaga atcccaaagt
    14341 gctaatggag tgagcgccct tacccactgt taccaggctt ccctgacccg cagtgatccc
    14401 gcctctctgc agaaactcag cattcttttc caggttaaat gtcgtgttct ggaacgccag
    14461 tgaaccctgg aaccagtctg tataagcaaa ctcatcgaag gagaattcgt gattccccat
    14521 gtttatttca acaatacctc taccttgaag atcgttatca aatacccaat ctttattggc
    14581 attgatttgc agtttgccat caacatccag gtccgcacgg ccgatgttct tgtcttcaga
    14641 aataaccgcg acagcaccag aagcgacatc aatatatccg ctgaatgcat agttattgcc
    14701 ggtcagcgcc actgtcccac tgtcgatact tattgtaccg ttgccggaaa tcttattggc
    14761 aaaggtcatg ctatcgtcag ccttcaggtg taatttcccg cggttgttga tttctgcggt
    14821 acctatattt tgggtgatcc ctaaagcgcc attttgctca attttgactt taccgctaaa
    14881 ggcggagtta tccccggtta agtaagtcgt accattgagc gcattgatat agcccaggcc
    14941 ctcgctgtca ctgacaatat ccgcggaaat agtcgcgttt ttatccgtgt ggtttaaggt
    15001 gattttcttc tcggatgtct ttgcccacac aaactcaatt ttttcggcat caataatccc
    15061 tgcagccttc gctgcgcttc cttcctgtgc acctaacgct aactcagagt tggtgcttaa
    15121 actaatagtc ggcgcagaga ttttaccacc ttccgaaaca gcaagtgttg tatcggagtt
    15181 ttgatcataa taacccaggc taattttctc actggcatta acttcaccgc cgttattaac
    15241 tgacaggtac tgattaccac cgtaactgcc gtaaacgccc ataaagaagg ttttagaatt
    15301 tacctggctg ccttcaccac tgacatttcc ctgcaaatag gtcagacggc tggtgccctt
    15361 aacatggatt ttgccgccgt tggagacatt aaaatagccg cgatcgtgtc tcatgcctga
    15421 gtttgactca tacaaatcat cagcatataa ctcgctgcca gcattggaga cattcaggaa
    15481 ccccg
//
LOCUS       DAZPLM010000090        15430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPLM010000090 DAZPLM010000000
VERSION     DAZPLM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.090"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHVC7_002371"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHVC7_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR2116399.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHVC7_002372"
     CDS             1598..4654
                     /locus_tag="ACHVC7_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR2116400.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHVC7_002373"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHVC7_002373"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR2116401.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHVC7_002374"
     CDS             5161..5349
                     /locus_tag="ACHVC7_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR2116402.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHVC7_002375"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHVC7_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR2116403.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHVC7_002376"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHVC7_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR2116404.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHVC7_002377"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHVC7_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR2116405.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHVC7_002378"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHVC7_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR2116406.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHVC7_002379"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHVC7_002379"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR2116407.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHVC7_002380"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHVC7_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR2116408.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHVC7_002381"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHVC7_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR2116409.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHVC7_002382"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHVC7_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR2116410.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHVC7_002383"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHVC7_002383"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR2116411.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHVC7_002384"
     CDS             complement(14591..14785)
                     /locus_tag="ACHVC7_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116412.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHVC7_002385"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHVC7_002385"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR2116413.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3895 a         3736 c         4087 g         3712 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg
//
LOCUS       DAZPLM010000091        15406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPLM010000091 DAZPLM010000000
VERSION     DAZPLM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.091"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHVC7_002386"
     CDS             <1..453
                     /locus_tag="ACHVC7_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2116414.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHVC7_002387"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHVC7_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHVC7_002388"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHVC7_002388"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR2116415.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHVC7_002389"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHVC7_002389"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR2116416.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHVC7_002390"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHVC7_002390"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2116417.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHVC7_002391"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHVC7_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR2116418.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHVC7_002392"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHVC7_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR2116419.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHVC7_002393"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHVC7_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR2116420.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHVC7_002394"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHVC7_002394"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR2116421.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHVC7_002395"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHVC7_002395"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR2116422.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHVC7_002396"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHVC7_002396"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR2116423.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHVC7_002397"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHVC7_002397"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR2116424.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHVC7_002398"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHVC7_002398"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR2116425.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHVC7_002399"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHVC7_002399"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR2116426.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHVC7_002400"
     CDS             13055..13435
                     /locus_tag="ACHVC7_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR2116427.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHVC7_002401"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHVC7_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR2116428.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHVC7_002402"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHVC7_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR2116429.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHVC7_002403"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHVC7_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR2116430.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4042 c         3851 g         3597 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taatggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZPLM010000092        15338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPLM010000092 DAZPLM010000000
VERSION     DAZPLM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.092"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHVC7_002404"
     CDS             <1..1015
                     /locus_tag="ACHVC7_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2116431.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHVC7_002405"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHVC7_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR2116432.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHVC7_002406"
     CDS             1797..1904
                     /locus_tag="ACHVC7_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR2116433.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHVC7_002407"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHVC7_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR2116434.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHVC7_002408"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHVC7_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR2116435.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHVC7_002409"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHVC7_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR2116436.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHVC7_002410"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHVC7_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR2116437.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHVC7_002411"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHVC7_002411"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR2116438.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHVC7_002412"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHVC7_002412"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR2116439.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIGSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHVC7_002413"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHVC7_002413"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR2116440.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHVC7_002414"
     CDS             complement(11226..11813)
                     /locus_tag="ACHVC7_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR2116441.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHVC7_002415"
     CDS             11983..12561
                     /locus_tag="ACHVC7_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2116442.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHVC7_002416"
     CDS             complement(12691..13452)
                     /locus_tag="ACHVC7_002416"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2116443.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHVC7_002417"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHVC7_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR2116444.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHVC7_002418"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHVC7_002418"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR2116445.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3744 a         4176 c         3837 g         3581 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag ataatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcggca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataa
//
LOCUS       DAZPLM010000093        15169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPLM010000093 DAZPLM010000000
VERSION     DAZPLM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.093"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHVC7_002419"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHVC7_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR2116446.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHVC7_002420"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHVC7_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR2116447.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHVC7_002421"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHVC7_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR2116448.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHVC7_002422"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHVC7_002422"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR2116449.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAECGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHVC7_002423"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHVC7_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR2116450.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHVC7_002424"
     CDS             complement(8214..9107)
                     /locus_tag="ACHVC7_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR2116451.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHVC7_002425"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHVC7_002425"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR2116452.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHVC7_002426"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHVC7_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR2116453.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHVC7_002427"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHVC7_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR2116454.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHVC7_002428"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHVC7_002428"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR2116455.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHVC7_002429"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHVC7_002429"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR2116456.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="ACHVC7_002430"
     CDS             complement(14275..>15169)
                     /locus_tag="ACHVC7_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR2116457.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3739 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgca ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAZPLM010000094        15165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPLM010000094 DAZPLM010000000
VERSION     DAZPLM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.094"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..862
                     /locus_tag="ACHVC7_002431"
     CDS             <1..862
                     /locus_tag="ACHVC7_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR2116458.1"
                     /translation="SIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLL
                     IALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLEL
                     TDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDF
                     LPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKTREA
                     TPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQ
                     STSKTAPQAK"
     gene            complement(1065..1418)
                     /locus_tag="ACHVC7_002432"
     CDS             complement(1065..1418)
                     /locus_tag="ACHVC7_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR2116459.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1556..3202)
                     /gene="groL"
                     /locus_tag="ACHVC7_002433"
     CDS             complement(1556..3202)
                     /gene="groL"
                     /locus_tag="ACHVC7_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR2116460.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3246..3539)
                     /locus_tag="ACHVC7_002434"
     CDS             complement(3246..3539)
                     /locus_tag="ACHVC7_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR2116461.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3815..5071
                     /gene="yjeH"
                     /locus_tag="ACHVC7_002435"
     CDS             3815..5071
                     /gene="yjeH"
                     /locus_tag="ACHVC7_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR2116462.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5093..5563)
                     /locus_tag="ACHVC7_002436"
     CDS             complement(5093..5563)
                     /locus_tag="ACHVC7_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR2116463.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5900..7336
                     /gene="aspA"
                     /locus_tag="ACHVC7_002437"
     CDS             5900..7336
                     /gene="aspA"
                     /locus_tag="ACHVC7_002437"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR2116464.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7454..8755
                     /gene="dcuA"
                     /locus_tag="ACHVC7_002438"
     CDS             7454..8755
                     /gene="dcuA"
                     /locus_tag="ACHVC7_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR2116465.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8871..9209
                     /gene="cutA"
                     /locus_tag="ACHVC7_002439"
     CDS             8871..9209
                     /gene="cutA"
                     /locus_tag="ACHVC7_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR2116466.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9185..10882
                     /gene="dsbD"
                     /locus_tag="ACHVC7_002440"
     CDS             9185..10882
                     /gene="dsbD"
                     /locus_tag="ACHVC7_002440"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR2116467.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10919..11494
                     /locus_tag="ACHVC7_002441"
     CDS             10919..11494
                     /locus_tag="ACHVC7_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2116468.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11601..11676
                     /locus_tag="ACHVC7_002442"
     tRNA            11601..11676
                     /locus_tag="ACHVC7_002442"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11634..11636,aa:Phe,seq:gaa)
     gene            11919..12033
                     /locus_tag="ACHVC7_002443"
                     /pseudo
     CDS             11919..12033
                     /locus_tag="ACHVC7_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12293..12592
                     /locus_tag="ACHVC7_002444"
     CDS             12293..12592
                     /locus_tag="ACHVC7_002444"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR2116469.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12583..12708)
                     /gene="laoB"
                     /locus_tag="ACHVC7_002445"
     CDS             complement(12583..12708)
                     /gene="laoB"
                     /locus_tag="ACHVC7_002445"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR2116470.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12775..13830
                     /gene="cadC"
                     /locus_tag="ACHVC7_002446"
     CDS             12775..13830
                     /gene="cadC"
                     /locus_tag="ACHVC7_002446"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR2116471.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14195..>15165
                     /gene="cadB"
                     /locus_tag="ACHVC7_002447"
     CDS             14195..>15165
                     /gene="cadB"
                     /locus_tag="ACHVC7_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR2116472.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3733 a         3777 c         3636 g         4019 t
ORIGIN      
        1 aagcatcaag ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc
       61 gctgaaaatt aaagaacctc gcaataaaga atcgttgttc tttatgtctg tcatggaact
      121 gcgcgacctg ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tgaatgctgc
      181 tgcacgcctt cagtatgagc aagctcgcga taaagtcatt aagaaaatgg cagaaaacat
      241 cccagaaatt ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct
      301 ggaacttacc gataatcaag gcgaaaatct taccttcgta ttaacgctac atgacggtag
      361 caaatgtgag ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca
      421 tgcaattaat aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt
      481 cctgccgtta tacgacgttg actgccagga caatggcaat ctggaatatg acacctattc
      541 ccaaccggaa tggaaacata acctgttcga ccattatctg gcggtgcttt accgctttaa
      601 agatgaaagt ggcaaagaac aatttagcgg cacagtggtg aaaacgcgtg aagcaacgcc
      661 gggtaaagaa atagaggcga tcactcgccg aatgcttgat ttcagcccac gactgaaaaa
      721 actcgcgggt gtgccatgtc aggtctatgt tcgcaccgta gcggcgaata acgcccaacc
      781 gctaacccag gatcaatgct tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa
      841 aactgcacca caggcgaaat aatctcatct ttgctaatgc gaggctggag ccatccagcc
      901 tgaaatgact gataacgtga tcgttggtca tgccggatgc gacgtaaacg ccttatccgg
      961 cctacaaatt ctcaagcatt caatcagttg caatgcctgc tgtaggcctg ataagcgaag
     1021 cgcatcaggc aataatacgt ttgtcaccca gcctctggat cacatcagtt cgggcactta
     1081 taaacctgac cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac
     1141 gggctgctga tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg
     1201 tttgctgcgc cgcgcataga accgccctct tctccgtgtt gcccggaaag ccagttgctt
     1261 tgcttacctg tcgcagtacc aatcagctgg cactctgcgc ctggctgctc gtccacaatg
     1321 cgtacactct gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc
     1381 gcggcaccga caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag
     1441 ctggacgcca ctcgcgtcgt ccgtgtctga atcttatact agaaagatga ccaaaagaaa
     1501 aacccccaga catttctgcc cgggggtttg tttatttctg cgaggtgcag ggcaattaca
     1561 tcatgccgcc catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat
     1621 catttttcgg caggtcggta accatgcact cggtggtgat catcaggcca gccacagaag
     1681 ccgcgtactg cagagcagaa cgggttactt tggttgggtc caggataccc atgtcgatca
     1741 tgttgccgta ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg
     1801 tgttagcaac aacagacggt tcttcgccgc agttcaggac gatctgacgc agcggagctt
     1861 ccattgcacg cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt
     1921 cagccagttt agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt
     1981 ctaccgcagc acgggtcgcg tgcagggcgt cttcaacgcg tgcttttttc tctttcattt
     2041 caacttcggt agcagcacct actttgataa ctgcaacgcc gcctgccagt ttcgctacgc
     2101 gttcctgcag tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct
     2161 gagcaacacg gccctggatt gcagcttctt cacccacgcc atcgatgata gtggtggtgt
     2221 ctttgttgat cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagtt
     2281 ccataccgat ctcttcagag atcacggtac cgccagtcag ggttgcgata tcctgcagca
     2341 tagctttacg acgatcgccg aagcccggag ctttaactgc agcaactttc acgatgccac
     2401 gcatggtgtt aacaaccaga gttgccagcg cttcgccttc tacatcttca gcgatgatca
     2461 gcagcggttt gcctgctttg gcaacggctt ccagaaccgg cagcatttcg cggatgttgg
     2521 agattttctt gtcagccagc aggatgaacg ggctttccag ttctactgcg ccagtttccg
     2581 gcttgttgat gaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt
     2641 ccagttcgtc ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt
     2701 tgtccatcgc ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac
     2761 caacctgagc aatcgcttta gagtcagagc acggtacgga cagcgctttc agttcttcaa
     2821 ctgcagcggt aacggctttg tcgataccac gtttcaggtc catcgggttc atgcccgcag
     2881 caacagcttt cagaccctca gtgatgatag cctgagccag tacagttgca gtggtggtac
     2941 cgtcgcctgc agcgtcgttc gctttagagg caacttcttt caccatctgc gcacccatgt
     3001 tttcgaactt gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg
     3061 tcggtgcacc gaaagattta tccagaacta cgttacggcc tttcggaccg agggtaactt
     3121 tcactgcatc tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga
     3181 attttacgtc tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc
     3241 gaggattacg cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca
     3301 ttgtcgatct tctcagattt cacaccgtag ccatcgttga aaataacgat gtcgccaact
     3361 ttcacatcca gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc
     3421 acttcgccgc gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat
     3481 ttagtttcaa cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt
     3541 gataactctc ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt
     3601 tcccggctgg tgaccagaga aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa
     3661 aaaaaattct gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc
     3721 aatttcttca tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt
     3781 tgcccaaaat ttgggcgcaa tcggatatca acccatgagt ggactcaaac aagaactggg
     3841 gctggcccag ggcattggcc tgctatcgac gtcattatta ggcaccggcg tgtttgccgt
     3901 tcctgcgtta gctgcacagg tagcaggcaa taacagcctg tgggcgtggc ccgttttgat
     3961 tatcttagtg ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc
     4021 aggcggtgtc gcgcacttcg tcggtatggc gtttggttcg cggcttgagc gagtcaccgg
     4081 ctggttgttt ttatcggtca ttcccgtggg tttgcctgcc gcgctacaaa ttgccgccgg
     4141 gttcggccag gcaatgtttg gctggcatag ctggcaactg ttgttggcag aactcggtac
     4201 gctggcgctg gtgtggtata tcggtactcg cggtgccagt tccagtgcta atctacaaac
     4261 cgttattgcc ggacttatcg tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa
     4321 acctgcgaat atccccttcc cggcacctgg taatatcgaa cttaccgggt tatttgctgc
     4381 gttatcagtg atgttctggt gttttgtcgg tctggaagca tttgcccatc ttgcctcgga
     4441 atttaaaaat ccagagcgtg attttcctcg cgcattgatg attggtctgc tgctggcagg
     4501 attagtctac tggggctgta cggtagtcgt cttacacttc gacgcctatg gtgaacaaat
     4561 ggccgcggca gcatcgcttc caaaaattgt agtgcaactg ttcggtgtag gagcgttatg
     4621 gattgcctgc gtaattggct atctgacctg ctttgccagt ctcaacattt atatacagag
     4681 cttcgcccgc ctggtctggt cgcaggcgca acataatcct gaccactacc tggcacgcct
     4741 ctcttctcgc catatcccga ataatgccct caatgcggtg ctcggctgct gcgtggtgag
     4801 cactttggtg attcatgctt tagagatcaa tctggacgct cttattattt atgccaatgg
     4861 catctttatt atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggacg
     4921 ttatcgacta ctggcggtgg ttggcgggct gttatgcgtt ctgttactgg caatggtcgg
     4981 ctggaaaagt ctctatgcgc tgattatgct ggcggggtta tggctgtttc tgccaaaacg
     5041 aaaaacgccg gaaaatggca taaccacata atccggcgtt tcgacattaa tcctagcgat
     5101 cgtctttatg atcaaggcgg tcgcggtcat catcctttcg ctggtactca ccatcaaaag
     5161 tattaccgcc accggtcccg gcgctaaaac cgccgcctgg catgcgagaa aagcgcaaat
     5221 gtggcatcaa cttcactgtc agatgctttt gcaccggcgg caataaaagc agaagaccga
     5281 ggaagtcggt gaaaaagccc ggtaataaaa gcagcaaacc ggcaataatc agcgaaacac
     5341 ttttaatcat ctccgccgca gggttttcac ccgccgccat tttttgctgc atcagcacga
     5401 aattcttaaa gccctggtta cgtaccagtg acataccgat aactgacgtg aatataacca
     5461 gcacgagggt cagcaatacc cccaatacat gggcaacctg aataaagatt gaaatctcaa
     5521 tatagacata aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata
     5581 gcagccggtt aaacccggct acctgaatgg gttgcgaatc gcgttcagct tatattgtgg
     5641 tcattagcaa aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg
     5701 atctatttca caaattaata attaaggggt aaaaaccgac acttaaagtg atccagatta
     5761 cggtagaaat cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg
     5821 tcggtcgaaa aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag
     5881 cttgaaaaag aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac
     5941 cagggaagtt ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt
     6001 ctatatcagc aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt
     6061 taaaaaagcc gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa
     6121 tgccatcatt gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc
     6181 ggtagacgtc taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct
     6241 ggccaatatc ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc
     6301 gaacgaccat gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat
     6361 cgcagtttac tcttccctga ttaagctggt agatgcgatt aaccaactgc gtgaaggctt
     6421 tgaacgtaaa gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga
     6481 cgcagtaccg atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga
     6541 agtgaaaaac atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc
     6601 aatcggtact ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc
     6661 tgaagtcact ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctctgactg
     6721 cggcgcttat gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat
     6781 ctgtaacgac ctgcgcttgc tctcttctgg tccacgtgcc ggcctgaacg agatcaacct
     6841 gccggaactg caggcgggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga
     6901 agtggttaac caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc
     6961 agaagcaggt cagctgcagt tgaacgttat ggagccggtc attggccagg ctatgttcga
     7021 atccgttcac attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat
     7081 cactgctaac aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta
     7141 cctgaacccg ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac
     7201 cggtaagagt gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga
     7261 cgatattttc tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga
     7321 tgaaagcgaa cagtaatcgt acagggtagt acaaataaag aaggcacgtc agatgacgtg
     7381 ccttttttct tgtgagcagt aacttaaaaa taacaatcta atatcaactt gttaaaaaac
     7441 aaggaaggct aatatgctag ttgtagaact catcatagtt ttgctggcga tcttcttggg
     7501 cgccagattg gggggaatag gtattggttt tgcaggcgga ttgggggtgc tggttcttgc
     7561 cgctattggc gttaaacccg gtaacatccc gttcgatgtc atttccatta tcatggcggt
     7621 tatcgccgct atttctgcca tgcaggttgc tggcggtctg gactatctgg ttcatcagac
     7681 agaaaagctg ctgcgccgta acccgaaata catcacaatc ctcgcaccga tcgtgaccta
     7741 tttcctgaca atctttgctg gtactggcaa catctctctg gcgacactgc cagttatcgc
     7801 tgaagttgcg aaggaacaag gcgttaaacc ttgccgtccg ctgtctactg cagtggtatc
     7861 cgcgcagatt gcgatcaccg catcgccaat ctcagcggca gtggtttaca tgtcttccgt
     7921 gatggaaggt catggcatca gctacctcca tctgctctcc gtggtcatcc cgtccaccct
     7981 gctggcggtt ctggtgatgt ccttcctggt cactatgctg ttcaactcca aactctctga
     8041 cgatccgatt tatcgcaagc gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca
     8101 gattgaaatc aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg
     8161 cgtggttatc tatgcaatca tcaacagccc aagcatgggt ctggttgaaa aaccgctgat
     8221 gaacaccacc aacgcaatcc tgatcatcat gctcagcgtt gcaactctga ccaccgttat
     8281 ctgtaaagtc gataccgaca acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc
     8341 ctgtatttgt atcctgggtg ttgcgtggct gggcgatact ttcgtttcca acaacatcga
     8401 ctggatcaaa gataccgctg gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat
     8461 cttcttcttt gcttctgctc tgctgtactc tcaggctgca accgcaaaag cactgatgcc
     8521 gatggctctg gcactgaacg tttctccgct gaccgctgtt gcttctttcg ctgcggtgtc
     8581 tggtctgttc attctgccga cctatccgac gctggttgct gcggtacaga tggatgacac
     8641 gggtacaacc cgtatcggta aattcgtctt caaccatccg ttcttcatcc cgggtactct
     8701 gggtgttgcc ctggccgttt gcttcggctt cgtgctgggt agcttcatgc tgtaatgacc
     8761 aatcgcgggg cgttcacgcc ccgctttctt tcccgccgac taacatcctt cccccgtccg
     8821 ttgtatagtg acctctctct tgcggttcca tctgttcttg cgaggtgttt atgcttgatg
     8881 aaaaaagttc gaataccgcg tctgtcgtgg tgctatgtac cgcaccggat gaagcgacag
     8941 cccaggattt agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc
     9001 ccggcgctac ctctctctat tactgggaag gtaagctgga gcaagaatac gaagtgcaga
     9061 tgattttaaa aactaccgta tctcaccagc aggcactgct ggaatgcctg aagtctcatc
     9121 atccatatca aaccccggaa cttctggttt tacctgttac acacggagac acagattacc
     9181 tctcatggct caacgcatct ttacgctgat cctgctactt tgcagcactt ccgtttttgc
     9241 cggattattc gacgcgccgg gacgttcaca ttttgtcccc gcggatcaag cctttgcttt
     9301 tgattttcag caaaaccaac atgacctgaa tctgacctgg cagatcaaag acggttacta
     9361 cctctaccgt aaacagatcc gcattacgcc ggaacacgcg aaaattgccg acgtgcagct
     9421 gccgcaaggc gtctggcatg aagatgagtt ttacggcaaa agcgagattt accgcgatcg
     9481 gctgacgctt cccgtaacca tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta
     9541 ccagggctgt gctgatgccg gtttctgtta tccgccagaa accaaaaccg ttccgttaag
     9601 cgaagtggtc gccaacaacg cagcgtcaca gcctgtgtct gttccgcagc aagagcagca
     9661 caccgcgcaa ttgccctttt ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt
     9721 tacgccatgc gtgctgccaa tgtacccact gatttctggc atcgtgctgg gtggtaaaca
     9781 gcggctctcc actgccagag cattgttgct gacctttatt tatgtgcagg ggatggcact
     9841 gacttacacg gcgctgggtc tggtagttgc cgccgcaggg ttacagttcc aggcggcgct
     9901 acagcaccca tacgtgctca ttggcctcgc catcgtcttt accttgctgg cgatgtcaat
     9961 gtttggcttg tttactctac aactcccctc ttcgctgcaa acacatctca cattgatgag
    10021 caatcgccaa cagggcggct cacctggcgg tgtgtttgtt atgggggcga ttgccggact
    10081 gatctgttca ccatgcacca ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag
    10141 cgggaacatg tggctgggcg gcggcatgct ttatctctat gcgttgggca tgggcctgcc
    10201 gctgatgcta attaccgtct ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga
    10261 acaagtcaaa accgcgtttg gttttgtgat cctcgcactg ccgatcttcc tgctggagcg
    10321 agtgattggt gatatatggg gattacgctt gtggtcggcg ctgggtgtcg cattctttgg
    10381 ctgggccttt atcaccagcc tacaggccaa acgcggctgg atgcgcgtgg tgcaaataat
    10441 cctgctggca gcggcattgg ttagcgtgcg cccacttcag gattgggcat ttggtgcgac
    10501 gcataccgcg caaactcaga cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt
    10561 aaatcaggcg ctcgttgaag ccaaaggcaa accggtgatg ttagatcttt atgccgactg
    10621 gtgcgtcgcc tgtaaaaagt ttgagaaata caccttcagc gacccgcagg tgcaaaaagc
    10681 gttagcagac acggtcttac ttcaggccaa cgtcactgct aacgacgcac aagatgtggc
    10741 gctgttaaag catcttaatg tccttggact accgacaatt ctcttttttg acggacaagg
    10801 ccaggagcat ccacaagcac gcgtcacggg ctttatggat gctgaaacct tcagcgcaca
    10861 tttgcgcgat cgccaaccgt gaacgacact tgcagtggga aagacggagg aaaatagcgt
    10921 gcaacgtgaa gatgtactgg gagaagccct gaaattatta gaattacaag ggattgccaa
    10981 caccacgctg gagatggttg ctgaacgtgt ggattatcca ctggacgagc tacgccgctt
    11041 ctggccagac aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc aacagataga
    11101 tgtctggcgt cgtcagctga tgcttgacga aacgcaaacc gccgaacaaa agctgctggc
    11161 acgttatcag gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct gtctatttat
    11221 cgctgcctgt acgttttatc ccgaccctgg ccaccctatt catcaactgg ccgatcagca
    11281 aaaaagcgcg gcctacgatt tcacccacga actgttaacc acgctggaag ttgacgatcc
    11341 ggcgatggta gcaaagcaga tggaactggt gctggaaggc tgtttaagcc gaatgctggt
    11401 gaatcgtagc caggcggatg tcgacaccgc acatcggctg gcggaagata tcctgcgctt
    11461 cgcccgctgc cgccagggtg gtgcactgac ctgacagaaa cacagaaaag aagcgatttg
    11521 ccgcaatctt aagcagttga atcgctttta ctgaaattag gttgacgaga tgtgcagatt
    11581 acggtttaat gcgccccgtt gcccggatag ctcagtcggt agagcagggg attgaaaatc
    11641 cccgtgtcct tggttcgatt ccgagtccgg gcaccaaatt catataaacg gacctccacg
    11701 gaggtccgtt tttcgtttca ggacgccacg atttaagcgt tctgcctcca aatcaattct
    11761 accgaactca accagattct ccccacatca ccagcatttt gcgggtatat cccaattcgg
    11821 gaaaatttgt tcctgagcga tagcactgac tgacgtgaaa tgtcgtgcgg ccccgtgatg
    11881 ttgttgaacg tcaaatgacg tcatcaggag cgtaacgcat ccataaagca caacaccggt
    11941 ctgaacggcg actgatgaga ttttctgaat gagaacaaag agaaatgtat cagtccgctt
    12001 gctcatgcaa agactaacaa tccattaaaa tagtaagcgc tccggacaat tttccatgga
    12061 ttattttcta cacatttttc tttggcaaag atgatgaatt ttgatggtaa ggaatattac
    12121 ttctggttct cagtaaaatc ctttcgtaat actatgtaat caagaagttt atggctggta
    12181 aaaataacgt cttgcattca ccaataacat gtaaataaac ccatctattg atggtaaaaa
    12241 taggtcatgg caattatcat tgcaccattc ccttttcgaa tgagtttcta ttatgcaaca
    12301 acctgtagtt cgcgttggcg aatggcttgt tactccgtcc ataaaccaaa ttagccgcaa
    12361 tgggcgtcaa cttacccttg agccgagatt aattgatctt ctggttttct ttgctcaaca
    12421 cagtggcgaa gtacttagca gggatgaact tatcgataat gtctggaaga gaagtattgt
    12481 caccaatcac gttgtgacgc agagtatctc agaactacgt aagtcattaa aagataatga
    12541 tgaagatagt cctgtctata tcgctactgt accaaagcgc ggctataaat gaatggtgcc
    12601 ggttatctgg tacagcgaag aagagggaga ggaaataatg ctatcttcgc ctccccctat
    12661 accagaggcg gttcctgaca caaattctcc ctcccacagt cttaccattc aaaacaccgc
    12721 aacgccacct gaacaatccc cagttaaaag caaacgattc actacctttt gggtatggtt
    12781 tttttcctgt tgtcgttagg tatctgtgta gcactggtag cgttttcaac tcttgataca
    12841 cgtcttccta tgagcaaatc gcgtattttg ctcaatccac gcgatattga cattaatatg
    12901 gtaaataaaa gttgtaacag ctggagttcc ccgtatcagc tctcttacgc gataggcgtg
    12961 ggtgatttgg tggcgacatc acttaacacc ttctccacct ttatggtgca tgacaaaatc
    13021 aactacaaca ttgatgaacc gagcagttcc ggtaaaacat tatctattgc ctttgttaat
    13081 cagcgccaat accgtgctca acaatgcttt atgtcggtaa aattagtaga caatgcagat
    13141 ggttcaacca tgctggataa acgttatgtc atcactaacg gtaatcagct ggcgattcaa
    13201 aatgatttac tggagagttt atcaaaagcg ttaaaccaac cgtggccaca acgaatgcag
    13261 gagacgctcc agcaaatttt gcctcatcgt ggtgcgttat taactaattt ttatcaggct
    13321 catgattatt tactgcatgg cgatgacaaa tcattgaatc gtgccagtga attattaggt
    13381 gagattgttc aatcatcccc agaatttacc tacgcgagag cagaaaaagc attagttgat
    13441 atcgtgcgcc attctcaaca ccctttagat gaaaaacaat tagcagcact gaacacagaa
    13501 atagacaaca ttgttacact gccggaattg aacaacctgt ccattatata tcaaataaaa
    13561 gcggtcagtg ctctggtaaa aggtaaaata gatgagtctt accaggcgat aaatactggc
    13621 attgatcttg aaatgtcctg gctaaattat gtgttgcttg gcaaggttta tgaaatgaag
    13681 gggatgaacc gggaagcagc tgatgcatat ctcactgcct ttaatttacg cccaggggca
    13741 aacacccttt actggattga aaatggtatt ttccagactt ctgttcctta tgttgtacct
    13801 tatctcgaca aatttctcgc ttcagaataa gtaactccgg gttgatttat gctctggaat
    13861 atttgttgtt gagtttttgt atgttcccat tggtataata tgttgcggca atttatttgc
    13921 cgcataattt ttattacata aatttaacca gagaatgtca tgcaatccat tgtaaacatt
    13981 gaatgtttat cttttcatga tatcaacttg cgatcctgat gtgttaataa aaaacctcaa
    14041 gttctcactt acagaaactt ttgtgttatt tcacctaatc tttaggatta atcctttttt
    14101 cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat agttatacat catgacccgg
    14161 actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga agatcgggct
    14221 atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg cattattacc
    14281 tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct ctattattgg
    14341 tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc aacaaggtgg
    14401 cccaattgct tatgccggag aaatttctcc tgcatttggt tttcagacag gtgttcttta
    14461 ttaccatgct aactggattg gtaacctagc gattggtatt accgctgtat cttatctttc
    14521 caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta ttgctatcgt
    14581 ctgggtattt acctttgtaa atatgctcgg cggtacctgg gtaagccgtt taaccactat
    14641 tggtctggtg ctggttctta ttcctgtggt gatgactgct attgttggct ggcattggtt
    14701 tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg gtcatgcgat
    14761 cattaaaagt attctgctct acctgtgggc cttcgtgggt gttgagtccg cagcagtaag
    14821 tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc tgggtactgg
    14881 tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt atccgtcttc
    14941 tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct tcaactatcc tcggtaactg
    15001 ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc tgggctcctg
    15061 gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact tcccgaaagt
    15121 ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgct
//
LOCUS       DAZPLM010000095        14922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPLM010000095 DAZPLM010000000
VERSION     DAZPLM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.095"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            115..357
                     /gene="yjbE"
                     /locus_tag="ACHVC7_002448"
     CDS             115..357
                     /gene="yjbE"
                     /locus_tag="ACHVC7_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR2116473.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            471..1109
                     /locus_tag="ACHVC7_002449"
     CDS             471..1109
                     /locus_tag="ACHVC7_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR2116474.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1106..1843
                     /locus_tag="ACHVC7_002450"
     CDS             1106..1843
                     /locus_tag="ACHVC7_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR2116475.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1843..3939
                     /locus_tag="ACHVC7_002451"
     CDS             1843..3939
                     /locus_tag="ACHVC7_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR2116476.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3986..4264)
                     /locus_tag="ACHVC7_002452"
     CDS             complement(3986..4264)
                     /locus_tag="ACHVC7_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116477.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4265..4408
                     /locus_tag="ACHVC7_002453"
     CDS             4265..4408
                     /locus_tag="ACHVC7_002453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116478.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4534..4944
                     /gene="psiE"
                     /locus_tag="ACHVC7_002454"
     CDS             4534..4944
                     /gene="psiE"
                     /locus_tag="ACHVC7_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR2116479.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHVC7_002455"
     CDS             complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHVC7_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR2116480.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6835..7725)
                     /gene="malG"
                     /locus_tag="ACHVC7_002456"
     CDS             complement(6835..7725)
                     /gene="malG"
                     /locus_tag="ACHVC7_002456"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR2116481.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7740..9284)
                     /gene="malF"
                     /locus_tag="ACHVC7_002457"
     CDS             complement(7740..9284)
                     /gene="malF"
                     /locus_tag="ACHVC7_002457"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR2116482.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9438..10628)
                     /gene="malE"
                     /locus_tag="ACHVC7_002458"
     CDS             complement(9438..10628)
                     /gene="malE"
                     /locus_tag="ACHVC7_002458"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR2116483.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10993..12108
                     /gene="malK"
                     /locus_tag="ACHVC7_002459"
     CDS             10993..12108
                     /gene="malK"
                     /locus_tag="ACHVC7_002459"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR2116484.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12180..13520
                     /gene="lamB"
                     /locus_tag="ACHVC7_002460"
     CDS             12180..13520
                     /gene="lamB"
                     /locus_tag="ACHVC7_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR2116485.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13672..14592
                     /gene="malM"
                     /locus_tag="ACHVC7_002461"
     CDS             13672..14592
                     /gene="malM"
                     /locus_tag="ACHVC7_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR2116486.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14821..>14922
                     /locus_tag="ACHVC7_002462"
     CDS             14821..>14922
                     /locus_tag="ACHVC7_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116487.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNF"
BASE COUNT         3708 a         3832 c         3917 g         3465 t
ORIGIN      
        1 aaacaccatc gctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
       61 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      121 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgt gtgggctgca
      181 cctgtacagg tgggcgaagc ggcaggggcg gcagcaacgt cggtttcggc ggggagttcc
      241 tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc ggcaaccggt
      301 ggcggtgatg gttctaatac cgggaccaca acaaccacaa ccaccagtac ccagtaataa
      361 agtatgtatc cccaaaataa ttcgagtcat tgcatctgtg gcttgaaata tgaagggatt
      421 aaccataacc acactccggt gtggttattc tgcccctctg gagaagagtc gtgaagcgac
      481 ctgcactcat tcttatctgc ctgttattac aggcctgttc agccacgact aaagagctgg
      541 gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg gacgatgata
      601 ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg caattgtttg
      661 tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc
      721 ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag
      781 tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat
      841 ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag
      901 tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc ctggttcgcg
      961 tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca
     1021 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa
     1081 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt tgcttttgag
     1141 tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca atggcagcag
     1201 tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtagggc aacctcgact
     1261 ggcaaacagc tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
     1321 gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcaccga
     1381 cgatgccgcc gccattaacg ccttacgcca gcaaatccag gcattggaag tgacaggcag
     1441 gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt
     1501 gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt tgttcgggct
     1561 tatcagccat cctggcaatc agccattcac tccaggtcgc gacgtggcga gctatctctc
     1621 tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtcgttt acccggacgg
     1681 acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg
     1741 cagcattatt tatgttggcc tcgcagactc cgtctggagt gagacgcctg atgcccttaa
     1801 cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg
     1861 cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata tcctgcgccc
     1921 attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt
     1981 atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac
     2041 tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgttacac cgacgtgcgc
     2101 acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc
     2161 ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt
     2221 gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc
     2281 tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat
     2341 gtgaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa
     2401 caagcgggat ctatcgacgg cagtcagatg ttccacggcc cggcctcact gtttggcggc
     2461 gtggaatacc agacgccctg gcaaccgctg cgcctgaaac tggagtatga aggtaataat
     2521 tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt cggggcgatt
     2581 tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg
     2641 tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcctacat cgataacgcc
     2701 cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcactcggt ggtggcgaat
     2761 cagttaacgt tgctgaaata caatgccgga cttgccgatc cgcagatcca ggcgaaaggc
     2821 gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc
     2881 cgtgctaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat ccgcattacg
     2941 gaaaatcgcc ttaacatgcc gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat
     3001 catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcaaaaacg cgtcgagcca
     3061 gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccat
     3121 atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg
     3181 ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tgctgaccat cggcagcctg
     3241 tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccgcc acaggactcg
     3301 cacttaccgc gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgc ctatgtgaat
     3361 aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctatgga
     3421 ggttatctcg aaaccatgtt tggcggtgcg ggggctgaag tgttgtatcg tccgctggac
     3481 agcaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca
     3541 aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcctactgg
     3601 acgccatctt tcgcccagga cgtgttagtg aaagccagcg tcgggcagta tctggcaggg
     3661 gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt ggtgggtggc
     3721 tatgccacga tcacgaatgt ttcaaaagag gagtacggcg aaggggactt caccaaaggc
     3781 gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg
     3841 attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg
     3901 tatgatatga ccagcgaccg tagcgtcaat ttccgctaag tcatgggaaa ggtgccagtt
     3961 ttcgcaatca ctggcaccct cattcttaat ggcctgttga gccatcagga acgcagttca
     4021 cccccggttt gcgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata
     4081 tcggttgcag gtctggttgc ctgagcgact gggccatcat tgggctggag acattgcttt
     4141 gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga agaacagaga
     4201 ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt
     4261 tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc
     4321 tgaatgggtg atttttgaat taccggcttg ggtgcggttt gtcttgccgg atgcgccgcc
     4381 aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg cgcgctccaa
     4441 atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt ttgcgttata
     4501 caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg tccgcgcgtg
     4561 gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg cctcggcctg
     4621 attttggttg tcttcctcgg caaagaaacg gtgcatctgg cagatgtgct gttcgcgcca
     4681 gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct ctatttcgaa
     4741 tttatcgcgc tgattgtgaa gtactttcag tccggttttc acttcccgct acgttacttt
     4801 gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa atcgccactg
     4861 gatgtgttga tctactcggc tgcgatcctg ttactggtga tcactctgtg gctgtgtaat
     4921 tcgaaacggc tgaagcggga gtaaaaagaa agcacgccga actggcgcgg cgtgctggac
     4981 aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc cagagggctt
     5041 ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata aatagtgctg
     5101 ccagaacgcc catacacccg taaatccagt aggagaaacc gttgtggaaa tgggccacca
     5161 gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc gccagccact
     5221 gggccgccac cgcgattgcc agcgctttac cacgaatagc attcgggaag atttccgaca
     5281 gcagtaccca gcataccgga ccccaggaca tggcaaaggc agcaacatag aacagcatcg
     5341 acagtagcgc cacaataccc ggtgcctgag tgtaaaacgc ggtaccgagg ctaaacatac
     5401 cgattgccat tccgagtgcg ccgataattt gcagtggctt acgaccaaat ttatccaccg
     5461 tcataattgc cagaacggtg aaggtgaggt tgataactcc gacaataatg gtctgcaaca
     5521 gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag tacagcacca
     5581 cattgatgcc gacaaattgc tggaagatgg agagcataac gccgatgaca atcacgccca
     5641 cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag tgtttaattt
     5701 cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata ctttccgcct
     5761 gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta tacagcagca
     5821 ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgccag ccgtcagtat
     5881 tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa actaaaagtt
     5941 gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata tgagctggag
     6001 ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca ataccgccaa
     6061 taatacgata aataacaaat tccgggacat aacctgccag ataaataggt actgtgttgt
     6121 ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca gaaataaaaa
     6181 acaggacagc agcaatctta agtgaatcac gacggccgaa gcggttactg caataaccac
     6241 cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat cctaacaggg
     6301 agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg agtgactcaa
     6361 cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat gtagcgacta
     6421 aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc tgccttagac
     6481 cattctgatc ttttatcagt gcattgatgt tggaattgtc aggcagttaa ggcaattatc
     6541 attttttaca atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga taattatcaa
     6601 aaattgatga aaacaatact attgccgtga ctcagagcac gaaagagaat tatcgtaagt
     6661 gggaaaacaa ataacgtaaa aataataagc tctatgatgg aaatattaac cggcgaacga
     6721 ttcagattgc agacgaaaga aaaaaaggcg ctccgtggag cgccgaataa cagtcacaag
     6781 ttgggataac gtaagttgag ggtgcagcgg cataacattg gcagaacaac atctttaacc
     6841 tttcacacca cctgccgtca ggccgttgac cagccagcgt tgagccagca agaagacgat
     6901 ggtgatcggt aatgcagaca tcacggcagc ggcggcaaag tcaccccaca ggtagttttg
     6961 cgggttgagg tattgctgca tccccacggc cagggtgtag ctgtttacgt cacgcagtaa
     7021 cagcgacgcg accggaactt cagtaatggc agcgataaac gacaggatga ataccaccgc
     7081 cagaatcggt actgacagcg gcaacaggac aaggcggaag gcctgccacg gtgtcgcacc
     7141 atccagcgca gccgcttctt ccagcgaact atcgatggtt tcgaaatagc ctttgatggt
     7201 ccagacatgc agcgcaatcc cacccagata cgcgaaaatt acgccgccgt gagtattcag
     7261 gccaatgaat ggaatgtact cacccagacg atcaaacaac gcatacaacg cgaccagtga
     7321 aagtactgcc gggaacatct ggaaaatcag cattcctttc agcagcgtcg ctttgcctgg
     7381 aaagcgcata cgggcgaaag cgtaggcgca ggtggtagag agcgcgacaa tgccaatcgc
     7441 ggaaatcccg gcgaccttta ccgagttcca tagccacagc agtaccggga atggcggtgg
     7501 cgtaatgcga ccatcagcct gttccacgct aaaacctaaa gccagtttcc agtgatccca
     7561 ggagatttgc tccgggatca ggctgccgat cgcaaagttc ccctggcgca gtgagatagc
     7621 gacgaccatc agcagcggga acataatcgc cgcgataaaa agtagcagta gcaggtgagt
     7681 aataaacaaa cgtgcttttt gcgatttcgg ttggaccatt gccatttttg ttatctccct
     7741 taatcaaact tcattcgcgt ggctttcagg ttcactatcg ccagcgcacc caccagcagg
     7801 aagagcagcg tggcaattgc tgcagccaga ccgaagtcct gacccccgcc gccttcaaaa
     7861 gcgatacggt aggtgtagtt aacaagcagg tcggtataac cggctggcgt ggtcgtgcca
     7921 agacgatccg ggccgccgtt ggttaacagt tgaatcagca cgaagttgtt aaagttaaag
     7981 gcgaagctgg cgatcatcag cggcgtcagc ggtttaatca gcagcggcag cgtaatctta
     8041 aagaagttct ggaacggacc tgcgccatcc attgctgagg cttcatacaa atcgtccgga
     8101 atcgctttca gcaagcccat gcagaggatc atcatgtacg gataacccag ccaggtattg
     8161 acgataatta gcatcgtgcg ggcggtggtc ggatcgctga accaggcggg cttcacgcca
     8221 aacagcgcgc tcaacatcat gttgatttca ccgaagctct ggttaaacaa ccctttgaaa
     8281 atcaagattg aaatgaatga tggcaccgcg tagggcagaa tcagcagaac gcgatagacc
     8341 gctttgccgc gcaatgcttc ccactgcacc agacatgcca gaaccatgcc gaccgccacc
     8401 gttaaaaaga cagtgatcag cgagaacacc acggtccaga cgaaaatggc gaggaacggt
     8461 ttctgaatgc cttcgtcggt gaagacgcgg gtaaagtttt tccagccagt agtcacggtg
     8521 taaccggggc ttagcttttc atcaccccag ttgccgtcgg cggtaatgga ctggtaaaag
     8581 ccaatttggt tattcggacg atatttcacg ccgctctgat tattcgtcaa cgtgccgtca
     8641 ccgtcgagtg tgtagagcgg ctgcgtgcca gaaaactggc gcagggagct catcatcact
     8701 ttgttgccat ccggcagaat ggcggtaatg tcactcagcg cctgacgatt ctgggtaatc
     8761 acgcgtagat tcgcgcgttc gccttcgggc tgggcggtcg tttctttcag ttgcagtttt
     8821 tgttcgccgc caaatttaaa cgcgtcggag aggtaatttt tgccgatttc gccgtcactg
     8881 agcgccagtt gccactcatc gcccgccggg taaagaccaa agttataggt tttgcctgct
     8941 tgccaggagc gatctaacaa cacttcctgc gcacattcaa aagtcagctg gttagtgctg
     9001 ctgtagttgg tgaaggcaat ggcgatggtg cagaccagag ggaagaggac gaataatccc
     9061 attccagcca ttcccgggta aacatagcgc caggcgtagg ctttacgatt ggcgaaaata
     9121 tacagccccg ctgaactcaa tatcagcgtg gtaatggcga acaggtattc cccttgtgcg
     9181 tacattaaaa caacaaggta acccaccagc aggccgagca gacctagcac tgaccatttc
     9241 agcgcgtcgc tttgccacca atgtttcttt ttaatgacat ccatggggtt cttcctcatt
     9301 ccaggacgga taaggctttc acgccttatc cgacaacaac tgcctgatgc gacactgatg
     9361 cgtcttatca ggcctacatg cgtttcagtt ttgtaggccg gacaaggcgt tcacgccgca
     9421 tccggcattt cacagcatta cttggtgata cgagtctgcg cgtctttcag ggcttcatcg
     9481 acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg
     9541 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat ggtggcggca
     9601 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgccactgc acccagcggt
     9661 ttgtctttat tgaccgcttc cagaccttcg tcagtcagca gatagttttc gaggaactct
     9721 ttcgccagct ctttgttcgg actggcggcg ttaataccgg cgctcagcac gccaacgaac
     9781 ggtttagatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg
     9841 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta
     9901 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaataagg
     9961 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacggccac gtctttaata
    10021 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag
    10081 gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct
    10141 ttatccagcg ccgggatctc ttcccaggtt tttggtgggt tcggcagcag atctttgtta
    10201 taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt
    10261 acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg ggtgatttca
    10321 gccaacaggc cagattgagc gtagccacca aaacggtcgt gcgcccagaa gataatgtca
    10381 gggccatcgc cagttgccgc aacctgaggg aatttctctt ccagtttatc cggatgctca
    10441 acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc gagaccgtta
    10501 tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt ggcgagagcc
    10561 gaggcggaaa acatcatcgt cgttaatgcg gataatgcga ggatgcgtgc acctgttttt
    10621 attttcataa tctatggtcc ttgttggtga agtgctcgtg aaaacaccta aacggactct
    10681 agtttcttta tacggcaacc tctttccatc ctccttgccc ctacgcccca ccgttgcttt
    10741 gtgtgatctc tgttacagaa ttggcggtaa tgtggagatg cgcacataaa atcgccacga
    10801 tttttgcaag caacatcacg aaattcctta catgacctcg gtttagttca cagaagccgt
    10861 gttctcatcc tcccgcctcc tcccccataa aaaagccagg gggtggagga tttaagccat
    10921 ctcctgatga cgcatagtca gcccatcatg aatgttgctg tcgatgacag gttgttacaa
    10981 agggagaagg gcatggcgag cgtacagctg caaaatgtaa cgaaagcctg gggcgaggtc
    11041 gtggtatcga aagatatcaa tctcgatatc catgaaggtg aattcgtggt gtttgtcgga
    11101 ccgtctggct gcggtaaatc gactttactg cgcatgattg ccgggcttga gacgatcacc
    11161 agcggcgacc tgttcatcgg tgagaaacgg atgaatgaca ctccgccagc agaacgcggc
    11221 gttggtatgg tgtttcagtc ttacgcgctc tatccccacc tgtcagtagc agaaaacatg
    11281 tcatttggcc tgaaactggc tggcgcaaaa aaagaggtga ttaaccaacg cgtcaaccag
    11341 gtggcggaag tgctgcaact ggcgcatttg ctggatcgca aaccgaaagc gctctccggt
    11401 ggtcagcgtc agcgtgtggc gattggccgt acgctggtgg ccgagccaag cgtatttttg
    11461 ctcgatgaac cgctctccaa cctcgatgct gcactgcgtg tgcaaatgcg tatcgaaatc
    11521 tcccgtctgc ataaacgcct gggccgcaca atgatttacg tcacccacga tcaggtcgaa
    11581 gcgatgacgc tggccgacaa aatcgtggtg ctggacgccg gtcgcgtggc gcaggttggg
    11641 aaaccgctgg agctgtacca ctatccggca gaccgttttg tcgccggatt tatcggttcg
    11701 ccaaagatga acttcctgcc ggtaaaagtg accgccaccg caatcgatca agtgcaggtg
    11761 gagctgccga tgccaaatcg tcagcaagtc tggctgccag ttgaaagccg tgatgtccag
    11821 gttggagcca atatgtcgct gggtattcgc ccggaacatc tactgccgag tgatatcgct
    11881 gacgtcatcc ttgagggtga agttcaggtc gtcgagcaac tcggcaacga aacccaaatc
    11941 catatccaga tcccttccat tcgtcaaaac ctggtgtacc gccagaacga cgtggtgttg
    12001 gtagaagaag gtgccacatt cgctatcggc ctgccgccag agcgttgcca tctgttccgt
    12061 gaggatggca ctgcatgtcg tcgactgcat aaggagccgg gcgtttaagc accccacaaa
    12121 acacacaaag cctgtcacag gtgatgtgaa aaaagaaaag caatgactca ggagatagaa
    12181 tgatgattac tctgcgcaaa cttcctctgg cggttgccgt cgcagcgggc gtaatgtctg
    12241 ctcaggcaat ggctgttgat ttccacggct atgcacgttc cggtattggc tggacaggta
    12301 gcggcggtga acaacagtgt ttccagacta ccggtgctca aagtaaatac cgtcttggca
    12361 acgaatgtga aacttatgct gaattaaaat tgggtcagga agtgtggaaa gagggcgata
    12421 agagcttcta tttcgacact aacgtggcct attccgtcgc gcaacagaat gactgggaag
    12481 ctaccgatcc ggccttccgt gaagcaaacg tgcagggtaa aaacctgatc gaatggctgc
    12541 caggttccac catctgggca ggtaagcgct tctaccaacg tcatgacgtt catatgatcg
    12601 acttctacta ctgggatatt tctggtcctg gtgccggtct ggaaaacatc gatgttggct
    12661 tcggtaaact ctctctggca gcaacccgct cctctgaagc tggtggttct tcctcttttg
    12721 ccagcaacaa tatttatgac tataccaacg aaaccgcgaa cgacgttttc gatgtgcgtt
    12781 tagcgcagat ggaaatcaac ccgggcggca cattagaact gggtgtcgac tacggtcgtg
    12841 ccaacctgcg tgataactat cgtctggttg atggcgcatc gaaagacggc tggttattca
    12901 ctgctgaaca tactcagagt gtcctgaagg gctttaacaa gtttgttgtt cagtacgcta
    12961 ctgactcgat gacctcgcag ggtaaaggtc tgtcgcaggg ttctggcgtc gcgtttgata
    13021 acgaaaaatt tgcctacaat atcaacaaca acggtcacat gctgcgtatc ctcgaccacg
    13081 gtgcgatctc catgggcgac aactgggaca tgatgtacgt gggtatgtac caggatatca
    13141 actgggataa cgacaacggc accaagtggt ggaccgtcgg tattcgcccg atgtacaagt
    13201 ggacgccaat catgagcacc gtgatggaaa tcggctacga caacgtcgaa tcccagcgca
    13261 ccggcgacaa gaacaatcag tacaaaatta ccctcgcaca acaatggcag gctggcgaca
    13321 gcatctggtc acgcccggct attcgtgtct tcgcaaccta cgccaagtgg gatgagaaat
    13381 ggggttacga ctacaacggc gatagcaagg ttaacccgaa ctacggcaaa gccgttcctg
    13441 ctgatttcaa cggcggcagc ttcggtcgtg gcgacagcga cgagtggacc ttcggtgccc
    13501 agatggaaat ctggtggtaa tagcaaaacc tgggccggat aaggcgttta cgccgcatcc
    13561 ggcataaaaa caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt
    13621 agcgcgctat tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg
    13681 aataaaagtc tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatt
    13741 agccttgccg atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct
    13801 gccgcgctgc aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa
    13861 ctggcgaccg gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac
    13921 agcgtcccgg caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa
    13981 accagcgttt ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc
    14041 ttccccagca gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa
    14101 ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc
    14161 acggaaaaag atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaaa
    14221 ggcgtcggta actcgatccc ggatattccc gatccggttg ctcgtcatac cagcgatggc
    14281 ttactgaaac tgaaagtcaa aacgaactcc agctccagcg tgttggtagg accactattt
    14341 ggttcttccg ctccagctcc ggttacggta ggcaacacgg cggcaccagc tgtggctgca
    14401 cctgctccgg tgccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt
    14461 aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt
    14521 gatgaagctg aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa
    14581 ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg
    14641 cacctttttt tatgcttcct tcctgggata tgagcgattt tttatagtac ttcacttctt
    14701 cttcactaag aatgtccatt atctcaatgc cttatcagag attcttttcc tttcgccggt
    14761 agtgtctgga cgctcaggct acttttccag gttattttat ttctgtatta cagaggtttt
    14821 atgataggtc atatcacaaa ttctggcggc aataactctt tgatgaaaca tgatgtggtt
    14881 caaggaaata atatagtaga tcttgattta ctacgtaatt tc
//
LOCUS       DAZPLM010000096        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPLM010000096 DAZPLM010000000
VERSION     DAZPLM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.096"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHVC7_002463"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHVC7_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR2116488.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHVC7_002464"
     CDS             complement(1556..1879)
                     /locus_tag="ACHVC7_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR2116489.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHVC7_002465"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHVC7_002465"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR2116490.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHVC7_002466"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHVC7_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR2116491.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHVC7_002467"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHVC7_002467"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR2116492.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHVC7_002468"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHVC7_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR2116493.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHVC7_002469"
     CDS             7479..8516
                     /locus_tag="ACHVC7_002469"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR2116494.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHVC7_002470"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHVC7_002470"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR2116495.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHVC7_002471"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHVC7_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR2116496.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHVC7_002472"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHVC7_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR2116497.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHVC7_002473"
     CDS             10687..11568
                     /locus_tag="ACHVC7_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2116498.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHVC7_002474"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHVC7_002474"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR2116499.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHVC7_002475"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHVC7_002475"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR2116500.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHVC7_002476"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHVC7_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR2116501.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPLM010000097        14554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPLM010000097 DAZPLM010000000
VERSION     DAZPLM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.097"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            33..389
                     /locus_tag="ACHVC7_002477"
     CDS             33..389
                     /locus_tag="ACHVC7_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2116502.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(440..1332)
                     /gene="yhaJ"
                     /locus_tag="ACHVC7_002478"
                     /pseudo
     CDS             complement(440..1332)
                     /gene="yhaJ"
                     /locus_tag="ACHVC7_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1437..2138
                     /locus_tag="ACHVC7_002479"
     CDS             1437..2138
                     /locus_tag="ACHVC7_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR2116503.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2161..2325
                     /gene="yhaL"
                     /locus_tag="ACHVC7_002480"
     CDS             2161..2325
                     /gene="yhaL"
                     /locus_tag="ACHVC7_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR2116504.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2459..3769)
                     /gene="cyuA"
                     /locus_tag="ACHVC7_002481"
     CDS             complement(2459..3769)
                     /gene="cyuA"
                     /locus_tag="ACHVC7_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR2116505.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3797..5128)
                     /locus_tag="ACHVC7_002482"
     CDS             complement(3797..5128)
                     /locus_tag="ACHVC7_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2116506.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5403..6767)
                     /gene="tdcG"
                     /locus_tag="ACHVC7_002483"
     CDS             complement(5403..6767)
                     /gene="tdcG"
                     /locus_tag="ACHVC7_002483"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR2116507.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6839..7228)
                     /locus_tag="ACHVC7_002484"
     CDS             complement(6839..7228)
                     /locus_tag="ACHVC7_002484"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR2116508.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7242..9536)
                     /gene="tdcE"
                     /locus_tag="ACHVC7_002485"
     CDS             complement(7242..9536)
                     /gene="tdcE"
                     /locus_tag="ACHVC7_002485"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR2116509.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9570..10778)
                     /gene="tdcD"
                     /locus_tag="ACHVC7_002486"
     CDS             complement(9570..10778)
                     /gene="tdcD"
                     /locus_tag="ACHVC7_002486"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR2116510.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10804..12135)
                     /gene="tdcC"
                     /locus_tag="ACHVC7_002487"
     CDS             complement(10804..12135)
                     /gene="tdcC"
                     /locus_tag="ACHVC7_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR2116511.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12157..13146)
                     /gene="tdcB"
                     /locus_tag="ACHVC7_002488"
     CDS             complement(12157..13146)
                     /gene="tdcB"
                     /locus_tag="ACHVC7_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR2116512.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13245..14183)
                     /gene="tdcA"
                     /locus_tag="ACHVC7_002489"
     CDS             complement(13245..14183)
                     /gene="tdcA"
                     /locus_tag="ACHVC7_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR2116513.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3788 a         3877 c         3379 g         3510 t
ORIGIN      
        1 ctttcgtcac ttttaattaa agggatgttt ttatgcagtg gtatttatcc gtattaaaaa
       61 attatgttgg tttctctggt cgcgcacgtc gtaaagagta ctggatgttt actctgatta
      121 acgccatcgt cggcgctatt atcaatgtca ttcaattgat tttaggtctg gagcttccat
      181 atctgtctat gctttatttg ctggcaacct tccttcccgt ccttgcgctt gctatccgtc
      241 gcttacacga taccgaccgt tcaggtgcct gggccttgct cttttttgtt ccgtttattg
      301 gctggctcgt gcttctggtt tttttctgca cagaaggtac ttctggcagc aatcgttacg
      361 gaaacgatcc gaagtttggt tcaaattaat cttagaattg gggcgatatt tcgccccttt
      421 ttattaacaa taataaatat tattttccgc taaaaagttt gggaatttcc cgcagacacc
      481 aggatttcgc ttcccccata ctgtcacgac gccaggccat aataatatcg atctcactgg
      541 tcgattccgg gctgacgaca cgcaaccgcc cttccgcaat atctttttcg accatcggat
      601 acggcatcgt cgccacgcca agccccgcca gtaatgcctg acgtttatct tcaatcgtgc
      661 tcaccgttaa gcgcggctgt ttgtccagca gttgtacggt caacaccggg cgctcacgag
      721 cggtatccgc taccgcaatt ccacgatatt tcacgcgcgt cacttcagat aacggttccg
      781 gctcctgatg aatcgggtga tcaggcgcgg caacgtagac gttcattaac gtatagagct
      841 tgcgcgagtt gatctccgac gaggaacgaa aatgcatatc cggcgcgata acaatatccg
      901 cccgcccctg ctccagtcgt tcctacgccc ccgccagcac ttcggtgatg attgccagtt
      961 gggtattggc ttttgccgcc agtttgtcga ttaacgggaa aaaggcaggt gtcggtacca
     1021 gcgcttcggt cacaatggtg agatgcgttt cccaaccgcg cgcgagggct tccgcatcgg
     1081 tagtcagttt atctgcggct tccagcaaaa cgcgccctcg ctccagcaac atcctcccga
     1141 cattggtgaa tttggtgcga tggcccgagc ggtcaaacag caccacatcc agctcttctt
     1201 ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg tcccagctca tccgccgccg
     1261 ccgcaaaact gccccggcga tcgcatccat aacccgtagt gcttccagcg ttaatgccct
     1321 ttctttggcc atttcgttct cattcaggaa atttgaacat accgggcaga atatctggct
     1381 aacaatgtag cgtccagccc cttaccataa aaggaagtaa agagaggtca agaattatga
     1441 ttactacccg aactgccagg cagtgtggac aagcagacta cggatggttg caggcccggt
     1501 atactttttc ctttggacac tacttcgacc cgaaattgtt aggctatgcc tccctgcgtg
     1561 tgcttaacca ggaagtgctg gccccaggtg ccgcctttca gccgcgaacc tatcccaaag
     1621 tcgatatttt aaatgtgatt ctggatggag aagcagagta tcgcgacagc gaaggcaatc
     1681 atgttcaggc cagcgccggt gaggcgttgc tgctctctac ccagccgggt gtcagctaca
     1741 gcgaacacaa tctcagcaaa gacaaaccgt taacacgaat gcagctttgg ctggacgcct
     1801 gcccgcagcg agagaatccg ctgattcaaa aactggcgct taatatgggc aagcagcaat
     1861 taatcgcctc gccagagggt acgatgggaa gcctgcaatt acgccagcaa gtgtggctgc
     1921 accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt
     1981 atttgcaatc gattcacggg aaatttcatg cgcttacgca tcatgaagag aaagcggcgc
     2041 tgacctgcgg tgatggggcg tttattcgtg acgaggctaa cattacgctg gttgccgatt
     2101 ccccactgcg cgctttgctg atagatttgc ctgtctagtt gtttttacag ggagatgatg
     2161 atgagtaaaa aatcggccaa aaagcgccag ccagtgaagc ccgtggtggc gaaagaacct
     2221 gctcgcaccg ccaaaaattt tggctatgaa gagatgttga gcgagctgga agccatcgtc
     2281 gcggatgctg aaacgcgttt agccgaggat gaagctaccg cgtaaagaca ggttgccgga
     2341 tgcggcgaaa acgccttatc cggcctacaa aatccaacaa cattaacgaa ttaaacaacc
     2401 cgtaggccgg ataaggtgcg ccagcatcgc atccggcaca atactcaaaa cctggcgttt
     2461 atctggcctt gctcgccata atctcgataa tctgccgatc cgtttgctgc atcgaatggc
     2521 ttgctaacgc acacaggttg gcaatcgact gctcaacatc atgcgccacg atcccttcat
     2581 tgccggtcac ggcggtatca tccagcgcca ttaacaccgc tttccacgca gccgaagcac
     2641 tggtcgaaac cttcatcgcg cagctgttcg acgcaccatc gcaaatcatg ccgctgacat
     2701 cgccgatcat actgctgatc gccatcgaga tagtttcata acgcccatcc accagccatg
     2761 ccatcccggc ggcggccccc attgctgcgg tcgttgcggc acaaagtgca gacaaacgcg
     2821 gtaactggtt atggatgtaa attgcgctca aatgagaaag catcagcgca cgcgccagtc
     2881 gttcatcatc cgctccgaag tgttctgcta caaccaccac aggcattgtt gcggtgatcc
     2941 cctggttacc cgagccggag ttactcatag ccggaagcgt agcgccgccc atacgcgcat
     3001 cggatgccgc gctggtacga atcacaatgg atgaagagag atctttcgcc agcaaaccgc
     3061 gctcgcactg tttttccagc gtcgcgccaa tatgcagccc ccacttaccg ctcaaacctt
     3121 cctgcgataa cgcacaattt agcttcgcgg aatcgagaat aaagcggatc gccgcaaacg
     3181 ggacttcatt gacgaacttc aggatctcag ccagcgtcgt tctggaaagc actgtcagcg
     3241 gagactcttg ctcgccctct gccacacacg cctgctgggt aaacaccaca ccattgtgcg
     3301 tctcaatatg cacaatgttg gtatgcccgc cgacgatggt gacacacgcc cacttctcac
     3361 cgttccagac tttggcgcgt gagaagagga tttcattgca aggttcctgg atcttaacgg
     3421 agactttccc cgccgccagc agtgctttgg catcggcaat tgcctgcgca gttgcgtctt
     3481 tcagcacttc cagcccggcg ttggcatttc cacctaacgc ccccagcgcc gccgcaatcg
     3541 gcagccccac cattcccgtg ccgggaacgg tgacgcccag accgttcttc atcagatttg
     3601 gcgaaaccca ggcttctaca cgttcaaccg gaccttccag ttctgccgca gcaaccgccg
     3661 ccgccagcgc cagtgaaatc ggttcagtac atcccagcgc cggttttact tcctcctgaa
     3721 cggcgaggat gtaacgctgc cataacggat ttaaagtcga atcaaacata ttaaaaacct
     3781 taaaatttca ggtaaatcaa gaaaatgcca ggaacggaga aacacaaagc aacaaaccag
     3841 tgacgataat caggtacaga gacatccctt tgtatttgtg caatgccggt actttgtaaa
     3901 ccagccacgc cgggatcagg caccctacca tgccgaaaat cgggctacag atagaggtga
     3961 agctcaacac cggtgcgttc agtacgatag cgctccaggc cagcaaaatg gcgaaaatca
     4021 tgatgccgcg ctgaacaaga ttttcgttaa tcttctcggc aggcatcttg cgacgcagga
     4081 tgttcattac gatcccttgc gttgcttcgc gaaagcctaa atagacgcca aagaatgcag
     4141 tcatgacagc aaagatattg agaatgacgc tgaccacttt cacccacgct gcgccgtcac
     4201 cgctaataaa ctgtgcggca atcgccagcg cggaaatatt ctgctcgtag gcttttaccg
     4261 cttcgtcatg ccccatcgcc agcgtgaacg acacggcgta gaaaaagacg gtgacaaaca
     4321 aaatgccaaa cgcgatattc attgcccgca atgctttatg ccgcgccact tcaatagatt
     4381 tttcccgcga gcgataagag atcaccatcg ggcttaacgt ctggataaac aaaatcgacg
     4441 tcagggtaaa cggcagcgta ataatggcgt ttttcaccag cagccccagc ggcggtagcg
     4501 aaccgacgtt atacagatgc cacattccca ccatcgacac gcccagcgcc gcgaccacca
     4561 gcagcttggt cagcaccatg ccggtcgaaa ttttgaataa caatttctcg ccgcgtgaag
     4621 agatcgccac cagaatgcaa atcagtacca gaccataaaa gggactgtct gacagcaacc
     4681 cttccgtcac gccgaaggta tgcagatagg aagcactatc gttggtgatg gcggtggaat
     4741 agacgaacat ccaaatcacc agcattacga aatagaacgc acctaacagg atgccccagt
     4801 ttttacctaa ataaccgcta atgacactcg ggtaatcttt acattctggt gattctgcca
     4861 gcgtattaat aaacaaccgc tgaaacagat acattgccgg gtaaccaatc accgatgaga
     4921 gcaaaaatac ccacaatccc atcaaaccga cctgcaccgg gagaaaaaca atccccgcgc
     4981 caatcgccat cccgatactc ataatcaccc agccggtgtc agtactgtcg aatttgatcg
     5041 cttctcgcca ctcgctctca ctcattccag cacgacccgc cggggtcgaa gcgtctgcaa
     5101 tgacgccttt attcgatgca atttccataa tttctcgctc aatattttgt agggcttatt
     5161 atttttttcc gagccgcatc aaggcgatat gcggtctacg tttttgcagg cgagttatta
     5221 gaataaagaa atgatacgcg cgaggtcaga gaaaatcttc acaatcaaga ctttgaaata
     5281 acggagatgg ataagaattt tctactaaat taatcgcaag agaatattca ttctctattt
     5341 gcggcagatc acaaaaaaaa ggtgcacatt tgtgcaccca aggatgaaag ctgacagcaa
     5401 tgtcagccgc agaccacttt aatggccagt cctccgcgtg atgtttcgcg gtatttatcg
     5461 ttcatatctt tgccggtttc atacatcgtc tcgatcactt tatcgagtga aacacgcggt
     5521 gccgaggtgc ggcgcatcgc catccacgcg gcgtttactg ctttcacggc attaatggca
     5581 ttacgttcaa tgcacgggat ttgtacctgt ccggcaaccg gatcgcaggt cagcccaagg
     5641 ttatgctcca tcgcgatttc cgccgcattg catacctgcg ccggactgcc gcccagtagt
     5701 tcagttaacc ctgccgccgc cattgaacag gccacgccaa tctccccctg acagccgacc
     5761 tccgcgccag agatggaggc gttcatttta tacagcgcgc caatagcccc cgctgccaga
     5821 aaatagcggg caattgaccg ctcgtttacc ggacgacgga acttatcgta ataagccagc
     5881 actgccggaa taatgccgca cgcaccgtta gtcggtgccg ttaccacgcg cccgccagct
     5941 gcgttttctt cactaaccgc cagcgcgtac atgttgatcc agtcgatgac attcatcgga
     6001 tcgttagaga tgttatcgct agaaaccagc tgacgacgca gcgctacggc acgacgcggc
     6061 acattgagcg gaccaggcag cacgccttca gtgttcatcc cgcgttcaat accgtcatgc
     6121 atcacttgcc agatacgggc aaatcctgcg tcaatttccg ctttgctgcg cagcgccagc
     6181 tcgttgtgca tcatcaggcc agatatagac aggccgttgt aatcacacat tttcagcagt
     6241 tcacctgctg agtggaaatc gtaaggtacg gacgtttcga catcgtgcga caggccgaag
     6301 tgttcttctt cgacaataaa cccgccgccg acagagtaat aggttttact taatagcgct
     6361 tcctgtccct tccaggcagt gatccgcatt ccgttctcat ggcgaggcaa catttcggga
     6421 tggaagataa tgttctttgc tacaggaaaa tcaacaatat gcgcaccaga tgccactggc
     6481 agccgcccgc tgcgcgttac taactctata aatgcaggga tctcatcaat gacaacatcc
     6541 tgcggactgt ttcctgccag tcccatgata atggcgacat ccgtggcatg gcctttgccc
     6601 gtcagtgaca acgacccata cagatcgacc acaatatggc tcgtcgcggt taataagccg
     6661 ctactttcca gccgatcaat aaaacttttt ccggcattca ttggccccac ggtatgcgaa
     6721 ctggagggac caatcccaat tttgaaaata tcgaatgcac taatcatatc cacaccctcg
     6781 gattgccgtt cagtgaagtg gagcggaacg accttgcgac cgtcccgctc acgaggcttt
     6841 acgcactacg tactgcgata gcttcaattt ccagcttcac atctttcggc aaacgcgcaa
     6901 cctgcacaca gctccgggtc gggtaggtcg cctgatgctc atcgaagaac tgcttataga
     6961 cttcattgat ggtggcaaaa tcattcagat cggtgatgaa cacggtcatc ttgatgatat
     7021 cgcccacgct cagcccggca gcaaccacga tcgctttgac gttttcgagg cttaaacgcg
     7081 cctgatcttg cacatcagcc gggatctcac cggtctgcgg gcaaaccgga atttgcccgg
     7141 aggtgaagac catgctgcct aaatcaacgc cctgaacata agggccgatt gcgcctgggg
     7201 cacgttgcgt ttcgataatc tttttcatac atcctccggc gtcagagcgc ctgggtaaaa
     7261 gtacgtgaaa taacatcctg ttgctgttca cgggtcagtg cgttgaagcg cacggcgtag
     7321 ccagagacac ggattgtcag gttaggatat ttttccgggt gctcgatggc atccagcagc
     7381 atttcccgat tcattacgtt gacgttgagg tgttgaccgc cttcgacatc cgcttcgtgg
     7441 tggaaatacc catccagcag gccgacaagg ttggttttac gtactggatc ttctttgccc
     7501 agcgccgcag gaacgattga gaaggtgtac gagatcccat ctttggcgta ggtgaacggc
     7561 agtttcgcca ccgacgtcaa tgaggccacg gcacctttgc ggtcacgacc atgcatcggg
     7621 ttagcgcccg gcgcgaacgg tgttccggcg cgacgaccgt ccggcgtatt accggttttc
     7681 tggccgtaca ccacgttaga agtgatagtc agaatcgact gggtagggac ggcgttgcga
     7741 taggttggca gcgctttaat tttcttcata aagcgttcaa ccaggtcgca ggcaatgctg
     7801 tctacgcgct cgtcgttgtt gccgtactgc ggatattcac cgtcgatttc aaagtccacc
     7861 gccaggccgt tttcgtcacg gattggtttc acgcgggcat atttgatggc agacagggag
     7921 tccgtcgcca ccgacaggcc cgcgatgccg catgccatag tgcgatagac atcacgatcg
     7981 tgcagcgcca tcagcgaagc ttcgtagctg tacttgtcgt gcatgtagtg aatgatattc
     8041 agcgcgctga tgtactgcac cgccagccag tccatgaagt gatcgaggct gtccatcact
     8101 ttgtcgtaat ccagcacgtc gtccatcagc ggtgctgttt tcggcccgac ctgaatcttc
     8161 agcttctcgt ccaccccgcc gttaattgcg tacagcagtg ttttcgccag gttagcgcgt
     8221 gcaccaaaga actgcatttg cttaccaatc accattgggc tgacgcaaca agcaattgcg
     8281 taatcgtcgc tgttgaagtc agtacgcatc agatcgtcgt tttcatactg caaggaagag
     8341 gtgacgatcg acacctgcgc ggcatatttt ttgaaggcaa tcggtaattc ttccgaccaa
     8401 agaatggtca ggttaggttc cggtgccggc cccatagtgt gcagggtgtg caaatagcgg
     8461 aaggagtttt tggtcaccag cgtacgaccg tccagcccca tcccgccgat cacttccgtc
     8521 gcccagattg ggtcgccgga gaacagcgaa tcaaattccg gcgtacgcaa gaagcgcacc
     8581 atacggatct tcataatgaa gtgatcgatc agttcctgtg cctgctgctc attgagtacg
     8641 cctgctttaa agtcgcgctc aatgtagata tcgaggaacg acgccgtgcg ccccagcgac
     8701 atcgcgccgc cgttctgtga tttcactgcc gccagataag cgaagtagag ccactgcacc
     8761 gcttcctgcg cattctgcgc cgggcgagag atatcaaagc catatttcgc cgccatttcc
     8821 tgaatctgca acagcgcatg acgatgctct gccagctcct cacgcagacg gatggtggct
     8881 tccagatctt cgcctttttc cagacgggac tggagatcgg caaattgcag ttcgcgttca
     8941 cgcaccagat aactgatgcc atacagcgct acgcggcgat agtcaccgat aatgcgccca
     9001 cggccatagc catctggtaa accggtcagc acgccagatt tacggcagcg cagcatatcc
     9061 ggtgagtaaa catcaaatac gccctggtta tgggttttac gcagatcggt aaacagatat
     9121 tcaaattcac tgtccatttc acggccatag gcgtggaatg aacttttaat catattaatg
     9181 ccaccgaacg gatgcagcgc gcgctttaat ggcgcatccg tttgcagacc aacaattttt
     9241 tccagcggct ggttaatata tcccgcatca tgagcggtaa ttgtggtggc aatattggta
     9301 tcgaaatcaa ccggcgcgtg ggttgcgttt tcgatacgga tgccttccat tactttttcc
     9361 cacaattccg tggtggcagg cgtcgcttcg gcgaggaaag attcatcgcc ttcatacggt
     9421 gtatagttat gttgaataaa atcgcggaca ttaatttcgt ttttccagtc cgtaccttta
     9481 aagccaagcc atgcgtcggc gtacagctta tcgctggtat caatatctat cttcatgaaa
     9541 aataatctct ctacaatact tcaactaaat tatgcaaatt ctgcgggcgc gttaactttg
     9601 cctaaatgaa tggcatccaa agcaatcatt ttttcttcgt tagtcggaat aacggcacaa
     9661 atgacacgcg cattttcact ggaaacaatt cgctctccaa aagagttaga gcgattattc
     9721 atttctgtat caatcactac gcctaataca gccaaatgtt ccatgaccag acgacgaatt
     9781 aaacttgaat tctctcctat tccgccggtg aatataattc catccaggcg acgtaatgaa
     9841 gctgcgtgtc cggcaatatg acgggcaatt cggtgaacaa aggttttaat tgccagttgc
     9901 gcgcgttcgt gaccttcatg ccaggctttt tccagaacac gtaaatccga agaaagaccg
     9961 gaaataccta ataatcccga ctctttattc actacgcgtt ccaggtcacc caggctctgg
    10021 ttggtttggc tggcgaccca ggacatcgca ccaaagtcga catcgccact gcgggttccc
    10081 atcatcaagc cttccagcgg cgtcattccc attgaagtat caacactctg accgttgcga
    10141 accgcgcaga ttgacgcgcc attgccaaga tgcgccacaa ccaggccgga gtcatcttcc
    10201 gccagattca gcagagaatg tgcgcgctgg gaaacatagc ggtgcgacgt gccgtggaaa
    10261 ccataacggc gtacacctaa ctcttcataa tatttccacg gcaggccgta taaataagct
    10321 tccggagcca tcgtctggtg gaaactggta tcaaataccg ccacctgagt tacgcccgga
    10381 aataattgct gcgccgattc aataccactt aaattggcgt aattatgcag gggtgccagt
    10441 ggagaaacgc gacggatatt atcaatgact tcatcggtaa taatggcgga ctcggtaaaa
    10501 atactgccgc cgtgagcgat gcggtggcca attaaggcca cactgtcatt taaattccgt
    10561 ttttccagtt caaatgcaat tgccttcaat gcaccttcgt agctgtggtg agccagcggt
    10621 gctggctctc ccccatttac ggataagaat gcattttccg agttaatacc gtcggcaata
    10681 cctgacatta atacttcaca gtcgctggca tcgagcacgg aaaacttaat cgaagacgaa
    10741 ccacagttaa taaccaaaac aaccggaaat tcattcatct cttttctcat cctgagttac
    10801 ggattaaaac agtttgtata cgatgttcag gatggtcagc agaccaatca cggtaacaaa
    10861 cacgttatcc agacgaccac ggtatttcgc cagagacggc gctttacgga tggcatacat
    10921 cggcaacagg cacagcaggg atgcgataat cggtgcgccc atggcttcaa tcaggtcgag
    10981 gatgttcggg ttggcgtagg caacaaccca ggtggagccc atgatgaaga tcatgctgat
    11041 agtgttcagt ttacccagcg acactttagt tttgtcgcct ttataaccaa acttcaggac
    11101 caggccattc agaccttcca gcgttcccag atagtgaccg aagaaagatt tgaagatagc
    11161 cacgagtgcg atgatggaag ccgcatattc cagtgtaatc gcgaacgttg ttttggtacc
    11221 ggtcatggac gcaaagtggt tagccagata agaaagcact ggaatattct gcgctttggc
    11281 ttccgccatg ttggccggag acagcgtaaa taggcagcta aaggcaaaga acatcaccac
    11341 tgcaaccatc agcatgctgg cacgagaaat gatttgggaa catttacgtt cggtgaagtc
    11401 gcgaccgaag tctttctcat actcttcacg tttagaaacc acgaaggaag agacgattgg
    11461 cgagaaatta aaggagaaaa ccatgatgga aatccccagc cagacggtga tcaggatacc
    11521 gtcatgaccg gttaacgaca gcgaaccgag gtcaacctgg tcgataactg cagagttcca
    11581 gtaagggatc agcgacagag aaatcagcac caggctggcg ataaacggcc ataccaggta
    11641 gctcatcact ttaaccatca gatccttacc aaaccagatg acgaaagcca tcagcagcag
    11701 caggaacagc gccacaaagc cgcgattcag cggtgcaaag ccgagctggt tttcccagaa
    11761 cgtcataaag gtattggtaa tagtaacgcc ataaatccac agcagtgggc aaatcgcgaa
    11821 gaagtacagg aacgtgataa ccacgccgcc agttttacca aaatgctctt ccaccgtttc
    11881 cgtaatgttg ccggaagggt tagagccaga aagacataga cgcgccagcg cccggtggca
    11941 ataaaatgcg atggggtatg ccaataccaa catcagaaga atcgggatca gtccgccaaa
    12001 acctgcgcgg atagggaaga acagcacccc ggcgccgatt gccgtaccaa acaagcctaa
    12061 cgtccatgtg gtatctgatt tacgccagga cgattgtttt gtctggctgg atacaatgct
    12121 atctgaagta ctcatatcct atcctcaacg aattaattaa gcgtcaacga aaccggtgat
    12181 ttgagagacg cgagaaagat cgatattgcc gccggaaata atactgacgg tttttctgtt
    12241 ttgaatatat tggtctaatt taccgcttaa taatgcagca catgccagag cgcctgcgcc
    12301 ttcggtgacg actttattgc gctgaattaa ggcaatcata ctgtttctga tttcgtcttc
    12361 gctgaccagc acgatgtcat cgactaattc acgaacgatt tcgtaagtta aattacccgg
    12421 gcgggagaca tcacaaccat ccgccagggt gccggtagtt cggtgcgtgg ttatttctcc
    12481 ggagtggaaa gaagccgcca tgccgtgaac gttttcagac tgtacgccaa taacacgaat
    12541 ggtcgggtta atagatttaa ttgccaccgc aataccagca attaaaccgc caccaccaat
    12601 tggcacaatc acgttatcga catcatagag atcttccata atttccagac caatcgttcc
    12661 ctggccagca atcactttcg gatcatcgta aggtgggata aaaatacggc cttccatttc
    12721 gacaatttcg ctcactttag cgatagtgtc gttgaagtta tcaccatgca gaacgacttc
    12781 tgcggagtag tcgcacgttg ccgctacttt ggattttggc gcaccttttg gcatcaccac
    12841 tttaccgtcg atacccagca tcgcgcagga gagcgaaacc ccttgcgcat ggttgcccgc
    12901 agaacaggcc accacgcctt tgcgtttttc cgcatcggtc agtgaactta atttattaaa
    12961 tgcgccacga attttaaatg aacccgtacg ctgcatattt tcaaacttca ggaatatttc
    13021 acctttgcaa cgttcactaa aatagttgga gcgaggcatg cctgttttat aaattcgccc
    13081 agccagtcgt tgtttcgctt caataatatc atcaatagca atcggcagat cgtatgtaat
    13141 atgcattata aaacctcttc gcctgaatta aatatgtagg taaccgtaac cgacacctgc
    13201 aaaacagaca ggtggattat ttatgtttaa ataaaaacaa ataactaacc aacttctatt
    13261 aattgccttc gtctacaccc attataagat gaatactctt tggctaattc caccaaaacc
    13321 gatgctgctt ttttaatacg ataatttttc gaccataccg cggcatattg tgccacaggt
    13381 aatgtttctt caaccggaat agtaataaat tgattagaac caaaaggtga cgtcatatca
    13441 caaggaatta cagttaagaa atcagcattg agaacaagat tataaattgt cacgactgag
    13501 tcggttttaa cgatgttttc aatactgatg ccatttcttt gtaacgtagt aagcagttcg
    13561 ctgtagtacc ccatattagt ttgtggcaac acccactgtt cgttcttcaa cgactccagc
    13621 gtggtggtgc cggtgcatgt tcgggactta ctggctacca gcacaaactc ggactcgaac
    13681 agcggctcaa catgtaaatc ctgaagcttc atttctgcac ttaacgtacc aatcgcaaaa
    13741 tccagtcgac cgtcgcggat tgccggtaag aacgaagaca gttgcgcttc atacatagaa
    13801 acctgcgctt tcgggaacac ctctttgaac ttgttgatca tccctgacat aaaagtaaaa
    13861 ccaatcaatg aaggaaaacc aaatgagact tccaccaccg cctcagaaga cataccgctt
    13921 atctcattaa ccatattttt catttcacgg gtaatggatt cggaacggga gagtaacaat
    13981 tgaccggcag gtgttaatgt tacaccggta tttttccgca ccactaattc cacaccaaaa
    14041 taatcttcaa tatcgttaat gattttactg acggccggtt gagttaaccc taattctttt
    14101 gcagccgagc cgatagagcc acttctaatg acttcctgaa agactaccag gtgctgcgtt
    14161 ttcggaagaa gaatagtgct cataacgacc tacgttaatt acctcattga cggcatgaag
    14221 tgtatcaaaa tgaaatgaac aggatatgtg cgaccactca caaattaact ttcaatactt
    14281 tccagggtat cgtcattaca attaaatacc tttaaatatc aacaagttaa agcataaaaa
    14341 tcagcataaa ccctgatttt tgtcaaaaaa aatgacgggg ataaccatat tttatggcga
    14401 taacatcatt cgttatgtgg ttaatacaaa aaaggggctg agaccatatt tcaagcaatt
    14461 accggataat taccaggcaa aatttgagtt aaatttaatg agcaagttct caaattttat
    14521 tataaataaa ccattttcag cgataaatac tgcg
//
LOCUS       DAZPLM010000098        14443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPLM010000098 DAZPLM010000000
VERSION     DAZPLM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.098"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHVC7_002490"
     CDS             <1..161
                     /locus_tag="ACHVC7_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR2116514.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHVC7_002491"
     CDS             complement(199..1260)
                     /locus_tag="ACHVC7_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR2116515.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHVC7_002492"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHVC7_002492"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR2116516.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHVC7_002493"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHVC7_002493"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR2116517.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHVC7_002494"
     CDS             complement(3454..4155)
                     /locus_tag="ACHVC7_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR2116518.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHVC7_002495"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHVC7_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR2116519.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHVC7_002496"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHVC7_002496"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR2116520.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHVC7_002497"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHVC7_002497"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR2116521.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHVC7_002498"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHVC7_002498"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR2116522.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHVC7_002499"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHVC7_002499"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR2116523.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHVC7_002500"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHVC7_002500"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR2116524.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHVC7_002501"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHVC7_002501"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR2116525.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3810 a         3695 c         3605 g         3333 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caa
//
LOCUS       DAZPLM010000099        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPLM010000099 DAZPLM010000000
VERSION     DAZPLM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.099"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHVC7_002502"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHVC7_002502"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR2116526.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHVC7_002503"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHVC7_002503"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR2116527.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHVC7_002504"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHVC7_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR2116528.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHVC7_002505"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHVC7_002505"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR2116529.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHVC7_002506"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHVC7_002506"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR2116530.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHVC7_002507"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHVC7_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR2116531.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHVC7_002508"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHVC7_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR2116532.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHVC7_002509"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHVC7_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR2116533.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHVC7_002510"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHVC7_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR2116534.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHVC7_002511"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHVC7_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR2116535.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPLM010000100        14159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPLM010000100 DAZPLM010000000
VERSION     DAZPLM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.100"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHVC7_002512"
     CDS             complement(<1..228)
                     /locus_tag="ACHVC7_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR2116536.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHVC7_002513"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHVC7_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            1187..1486
                     /locus_tag="ACHVC7_002514"
     CDS             1187..1486
                     /locus_tag="ACHVC7_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116537.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="ACHVC7_002515"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="ACHVC7_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR2116538.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /gene="yigI"
                     /locus_tag="ACHVC7_002516"
     CDS             complement(2472..2939)
                     /gene="yigI"
                     /locus_tag="ACHVC7_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR2116539.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="ACHVC7_002517"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="ACHVC7_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR2116540.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="ACHVC7_002518"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="ACHVC7_002518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR2116541.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="ACHVC7_002519"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="ACHVC7_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR2116542.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="ACHVC7_002520"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="ACHVC7_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR2116543.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="ACHVC7_002521"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="ACHVC7_002521"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR2116544.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="ACHVC7_002522"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="ACHVC7_002522"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR2116545.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="ACHVC7_002523"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="ACHVC7_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR2116546.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="ACHVC7_002524"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="ACHVC7_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR2116547.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="ACHVC7_002525"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="ACHVC7_002525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR2116548.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13674..13796)
                     /locus_tag="ACHVC7_002526"
     CDS             complement(13674..13796)
                     /locus_tag="ACHVC7_002526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116549.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(13837..14082)
                     /locus_tag="ACHVC7_002527"
     CDS             complement(13837..14082)
                     /locus_tag="ACHVC7_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR2116550.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3427 c         3689 g         3597 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgac
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAZPLM010000101        14046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPLM010000101 DAZPLM010000000
VERSION     DAZPLM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.101"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..467)
                     /locus_tag="ACHVC7_002528"
     CDS             complement(<1..467)
                     /locus_tag="ACHVC7_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116551.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIR"
     gene            797..1138
                     /gene="mobC"
                     /locus_tag="ACHVC7_002529"
     CDS             797..1138
                     /gene="mobC"
                     /locus_tag="ACHVC7_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFR2116552.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            1135..5346
                     /locus_tag="ACHVC7_002530"
     CDS             1135..5346
                     /locus_tag="ACHVC7_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR2116553.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPLQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTYHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQATEQTQATEQAQATEQTQATEQAQATEQAQATEQAQATS
                     SYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVAS
                     FDNENDAKEFCNIVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDL
                     NEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEG
                     ALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHG
                     LNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMI
                     SGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYRLAVAGKHGLERGDAVARVDVTE
                     QQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGL
                     NAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERH
                     EIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDAL
                     GKQFVQAREDHQESQQQRDDSQDMH"
     gene            complement(5389..5829)
                     /locus_tag="ACHVC7_002531"
     CDS             complement(5389..5829)
                     /locus_tag="ACHVC7_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116554.1"
                     /translation="MKEYEKTALIYSLKESNTLVLRIILISILVVGVISGAIYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAI
                     ITISFITMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(6074..6517)
                     /locus_tag="ACHVC7_002532"
     CDS             complement(6074..6517)
                     /locus_tag="ACHVC7_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116555.1"
                     /translation="MFFLYETYNFFYYLIKLIVIQPQYICVYMIFFFFNAGIAYSITN
                     DIEDQVCRWLLFVSMLHALMIPPAVIMPLIMPPKEILQETEKRQELHESIPKTCKLKA
                     LDAQQGGLFGVDKDEWVFPDNKSFYLPEKCRPENRITELAMMKEG"
     gene            complement(6663..6869)
                     /gene="hha"
                     /locus_tag="ACHVC7_002533"
     CDS             complement(6663..6869)
                     /gene="hha"
                     /locus_tag="ACHVC7_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR2116556.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNCLPPSELESFNAAAD
                     HRLAELTMNKLYDKVPPSVWKYVY"
     gene            complement(6988..7326)
                     /locus_tag="ACHVC7_002534"
     CDS             complement(6988..7326)
                     /locus_tag="ACHVC7_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116557.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTTVSSVANSFGIVS
                     SLAKSIGISFPTGNVIETCFFGMFSLLLAAFSFVSTMGLANAVPPIKNVFPLLFFVLV
                     LITNYNIYFR"
     gene            complement(7801..9957)
                     /locus_tag="ACHVC7_002535"
     CDS             complement(7801..9957)
                     /locus_tag="ACHVC7_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFR2116558.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAEKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            complement(9969..10556)
                     /locus_tag="ACHVC7_002536"
     CDS             complement(9969..10556)
                     /locus_tag="ACHVC7_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFR2116559.1"
                     /translation="MYLHIVPKLFHRMANKCTLKSISIPELDLIIDGESLSVGRPWPN
                     KCLWVGMRKSRKAVNGLLLHTDKKLRWFTTRYTWDIEDMGLIHHQVNTYIEDNEFDMV
                     SQEILLNGSFDKWSDRVHSAYEGKPPAIIQPKMESFLKRPGEDSHDMWEEFEWGNFLI
                     SREESILLHTIQSERLTTDFSLFKRQPSIESALVI"
     gene            complement(10583..10912)
                     /locus_tag="ACHVC7_002537"
     CDS             complement(10583..10912)
                     /locus_tag="ACHVC7_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116560.1"
                     /translation="MVTIIIGVSRVIDYKMGGVSVDIPPFPEWNSGHYIEIPGEGIVF
                     TGEKDDDGKRCYSGVIESHYSTDDWAFILSEYKNWLNDKERKFSYLFVLCNGELVYHE
                     VNKSELL"
     gene            complement(10930..11178)
                     /locus_tag="ACHVC7_002538"
     CDS             complement(10930..11178)
                     /locus_tag="ACHVC7_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116561.1"
                     /translation="MHSSFGLPYPAGHWFYSLQDLLDNPVFMVSFFVFWVATGQFLLT
                     TAHRKFNISETVEMVIIALLMILMTLSFYLCAILKASF"
     gene            complement(11214..11618)
                     /locus_tag="ACHVC7_002539"
     CDS             complement(11214..11618)
                     /locus_tag="ACHVC7_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR2116562.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            complement(11634..12169)
                     /locus_tag="ACHVC7_002540"
                     /pseudo
     CDS             complement(11634..12169)
                     /locus_tag="ACHVC7_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(12199..12852)
                     /locus_tag="ACHVC7_002541"
     CDS             complement(12199..12852)
                     /locus_tag="ACHVC7_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116563.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSNINNIKEIYSNDDLNCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLKIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            complement(12864..13988)
                     /locus_tag="ACHVC7_002542"
     CDS             complement(12864..13988)
                     /locus_tag="ACHVC7_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR2116564.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
BASE COUNT         4178 a         2766 c         2949 g         4153 t
ORIGIN      
        1 cgtatataat ttccaagagt attgctaact tgtacaaagt tagcacaatt ccaagatttt
       61 attgtttcta tagatacatt gttatctata gcttgttgag ctatctttgc attgttgaag
      121 caaactggca aatatgcatt aagtatatca ttatcttgct tcgtttttct atttttgtcc
      181 tcaatggtta ttgctttttg atacgttttc tctttatttg aaagaagatg agaaacatct
      241 tttttctcgg ccctttcttt tcgtatgcga tgcaatatgt tttcaaacgt ctttaacgtt
      301 agatcaaaac cttcatcatt aagagcatta acaatatcaa tgtttcttat gccttgttgc
      361 tgcgcttctt ctatttcagc aaaacgctct cttattcttc cagttttact tcgcttgacc
      421 ttctgagcta agagcgaacc aagtagttta ttttctccat tgttcatttg tcagtctcca
      481 ggacagtgaa atcatgatga gcaattctat catgaacgtc aagcaatttt tgctatgtat
      541 atgctatgtg tttgctatgt atgtgctatg tcattggttt ttatggtgtg tttttgctat
      601 gtattagata tgtttatggt gtgcatttga tgtgtgaatg ctatggaatc aaataatttt
      661 ctcaaacaca ggcacgatac cgattgtaca caagaccgtt ccggtcatgt tacaatcgtg
      721 tgccaaaggg ggatgcccct ttgaaacccc gaacgacgca gccgttctta tgcggttcta
      781 cgaaccttga ggacatatga agaaaaaaac caacaaaaac gttcatgtaa cattcagact
      841 taccgaagaa gaatatgctc cgttcgatag ggctataaaa gagcttaata ttagtaagtc
      901 agaatttttc aggctgctta ctattggtaa aataaacaca tatgcatctg ataaacgtaa
      961 catcccagaa tacaaacgtt gtctttctca gttgagttgg gcaggaaaca acataaatca
     1021 aatagcgcac cgattaaatt cagatcattt aaaaggtatt atatcagaat cgctttataa
     1081 aaaggtttta aatggactaa tcggtattcg tgatcgcctt caggagatag ctaaatgatt
     1141 gtcagatacg gtggtggtaa tgatggtatc gttgattatc tgataaatgg tcgcaaagca
     1201 gaacgccagt acacgcgtga tgaacttgat catcgtgtcg ttcttgatgg tgatttacag
     1261 actacagata agattattga ttctattgaa aacaaaagtc aggaacgtta tttgcacatt
     1321 actctgtctt ttcatgaaag tcatgtgtcg aatgaagtgt taaaggctat tgttgatgat
     1381 tataaaaagt tattgatgaa tgcttatcat cctgatgaat actctttcta tgcggaagct
     1441 catttgccta aaattcgtca tatccaggat aatagcaccg gagaacttgt tgaaagaaaa
     1501 ccacatattc atattgtaat tcctaaagta aatttgatta ctgaaaagtt cctgaatcca
     1561 gtgggagatg ttaccaaagg gcatacaatt gaacagcttg atgctataca ggaatttatt
     1621 aataataaat ataatcttga tagccctaaa gactatccgc gtaaagatgc ggattacgga
     1681 aaaattataa gtagagttaa gggagatctt tacaaagagc atcactctga attgaaaggt
     1741 gagttgcttt cgcgtataga gaacgagaaa attgaaaatt actctgtatt caaagatatt
     1801 gttgcagagt atggtgaact tcgtataaga aatgctggta aaaccaatga gtatctggct
     1861 gtaaaattgc cgggagataa aaaatacaca aacttaaaaa gtcctctttt tcgtcagaat
     1921 tacattgaga caaggacatt aacacttgag aaaccaacac acaaagagat agaaaagaga
     1981 cttaatacct ggttaaataa aacaagccaa gagattaagc atatttttaa ccaggcggag
     2041 aaaacaaggg aactttacaa gacattaagc cctttacaac aaattgattt cctgcaagag
     2101 aggataaaag aatatgactc aagagaaaaa cttaacgaaa gaaattctca gcaaacgtca
     2161 ggacgagagg gaggttacaa gtcgtgtcct aaaaagtttg ccagaatccg tcaatctgaa
     2221 gcaacagtcg gattgtcacg tatgccccaa cgcggtatgg tttacggaat caatggattc
     2281 acaagacccg actctgtcag tgtattgtca gatatttcgc agcgtgatct ggcagagcaa
     2341 ttaccgcaaa gagaacatcc tggtcaggat gtgcgacggg attatgatag acaatttaca
     2401 gagtcaggaa taaaaagtct tgaacgctct tcttttttgt gtgaaacgat gtttcagact
     2461 ctgaatgagg cggcagagaa aaacgaaatt acaacgatgg cagaaattcg cagaaatatt
     2521 gatcctgtca ggtttctgtc ttctgccgcc gagcgtttta atattattcc agcgcagcat
     2581 aaaatcagaa cagcaaagga cggttccccc cggttttctg tggggaatcg taatatgaac
     2641 gcatctgatt ttctgacaaa gcatattaat cttgcctgga aagatgcgaa atcctttctg
     2701 cttgaagttt attcacagca gttagaaaac acgccatata cgcgctatcc aacctacaga
     2761 cgccttacgt atcatgaagc ccgtgagcgt cttaactcac tgaatttatc ggaaaaaaca
     2821 ttacgaaata ctatcaggtt tgagcgtggc aagctgtata atgatctgcg agagatgaga
     2881 cgcgagttga agttaatacc acgtgaacag cgtgatattg ccgtgggtgt gattgtttac
     2941 aaaaaactaa ccacacttga gcgtctttct gaactcgaca cagaggggcg acacattatt
     3001 cgtcaatatc atgctgactg gcataaggat aaagatgaaa tgaaagccct tgaacgtctc
     3061 aaaagctatc tcaacttcga tgaaatcaac gccatttctg ctgacgaacc cgaactttcg
     3121 cttcagaaag cggtggattc ccagcgccgc ttagaagagg cgaaaaaggt taactccaaa
     3181 ctaaaagatc ttgtgatgga taagcaggat tccaggattg tttatcgcga tcaggaatcg
     3241 gaaaagcctg tctttaccga caaagggaac tttgtcgttg cgggtaaaaa tccctcaaaa
     3301 gaagagatcg gaataatgct tgagtattcc agggaaaagt ttggtggtgt actcaaactt
     3361 accggctctg aagatttcaa aaaaatgtgc gctgaagttg ccgcagagca ggacatgaag
     3421 attattttac gcccggagca gtatcaacag atgatgctgg aattaaaagc agaacttcag
     3481 ggtaataagt ttgagcaggt ggaaacacag gaaaatagcc aggagtccga atccagaata
     3541 gagaaaggtg atgcattgaa ggaacaggcc accgaacagg cacaggccac agaacagact
     3601 caggccacag agcaggcgca ggccacagag cagacgcagg ccacagagca ggcgcaggcc
     3661 acagagcaag cacaggccac agagcaagca caggccacat cttcatatga tcctggtgtt
     3721 attaccaggg caaataccct ggattcacag atgcttagta aaggcacaaa tggtgaattt
     3781 ggctatttga aatctctgga tagcgatgaa aatgagattt gggaagtgct ggggcatgta
     3841 cctggagaca gcgatgatat ctttagtgtt gccagttttg ataatgaaaa cgatgcaaaa
     3901 gaattttgca atattgttaa tgaattagga attgacagaa cacaggctct gattcaggag
     3961 cagcttacta ctcaacacga tcaggctact gcgcaggtgc ataataaaca ggaaatttat
     4021 tgtataaact ttagtcgctt ccatgatttg aatgaaggta tcgtctttca ttctaaagat
     4081 gcggctattc agtgttatga ggaaagcaaa tcttctgcaa tagaaaaata tgaaagcaat
     4141 tatttaaatg gtgatggttt tgatactgtt gtgcttatgt ctaaaactgt ctcaaccgat
     4201 gaattatcct cttatccaga gggggcatta gtaacgtttg ataggccatt tgaaataatt
     4261 gccaattcat atgaagaata ccgtacccca gtgtatgcgg tttctttttc gaaggatgag
     4321 tttagtgaag atattaagac ttttgagtca ttgcacgacg catctgaata taaaaataag
     4381 atgcttcagg agcacgggct taaccaggat gatatcttaa tcacacctgt aacaagagaa
     4441 gaaatcgcct ttaaaggaat taaggacgct gttaatgacg ctaatatggc agtgatggaa
     4501 caggcggggg attcgtccag agaatcacca gaagagatac tcgccagtat ttcagctaac
     4561 gagcatatga tctccgggct tgaaaacttc ctggttaaag accgttctca gttcagttcc
     4621 tgcaatgggg atatcgttgt ggaggcggaa attaccaggg gggaaggtgg tctttatcgc
     4681 cttgcggtcg ctggtaagca tggcttagag cgtggtgatg cagttgccag agtggatgtt
     4741 acggagcagc aatttgccgc gatcactgga aaaacacctt ctgaagtttt aacgggggac
     4801 caaacatcgg ctcgtgttcc ggtaattaca ggtatccatt tcagtacaag ggctattgaa
     4861 aatgtgaata aactggaaca acagaaggat tatgtctatt tctcaacaca tgaaggtctg
     4921 aatgctgaaa taaaagattt cagctctctc aaggatgcta ttgagtgggg ccgggttgaa
     4981 tgcgagctac atgatctcaa taagcgcgat actgtcattt atcgcgtcga gtctgaacat
     5041 atttctcagg ggattgacgc tgtaatgaaa aatgctgaac gcgtggagcg tcatgaaatt
     5101 gagaaagcac agggacggga ttgcacgccg gaagacggca agattcttga ggcaattgat
     5161 cgctttgagg acaagttcag aggggaagga ctgaagttcg aaagggagaa ggccgaatcc
     5221 gatttgctta atcacggatt cactcgtgaa atggcagaag atgctcttgg aaaacaattt
     5281 gtccaggcca gagaagatca tcaggaatcg cagcagcagc gtgacgattc acaggacatg
     5341 cactaattaa gaaaaaagcc cggttttcgg gctttttaag aaaaaaagtc aaccccaaac
     5401 agaaataata gctacaaaaa tggacagtaa taatgcaata aaagtaatgt tgagccatat
     5461 gttggacgag tcttttaaga ctaatatggc tgttaatgcc attgtaatga aactaatggt
     5521 aataatggct gcaaataaat attgtacaat gttttttatt gtacttggtt gctccccaat
     5581 tctttctctg taatttgcat caacattaga tatccattga agtaatagag caataaaaca
     5641 gcctactccg caaaaggtga attgcacaga cgttgggttt gaatcaaaca acgagatcgc
     5701 taagttatat aagtatatcg cacctgaaat caccccgaca actaaaatag agattagtat
     5761 aatccgaaga actaatgtat tgctttcctt taaggaatat attaaagccg ttttttcgta
     5821 ttctttcatg atttaatccg gtctataagt ataagaacca acaaaacttt ctcaacctca
     5881 acgttaacat caagacacct cacttcataa atgatcgcct cagtatgtca aacagaaaaa
     5941 ggcaaaaagc actggcggac agacacggta gtgtttcaaa tggatttttt gacaaaatgc
     6001 cgtagccagc gaaaaaaaga ctaaacagaa gggggataca aagaacaaac aatttgaata
     6061 tcttcataga tctttatcct tctttcatca tggctagttc cgtaatcctg ttttctggac
     6121 ggcacttttc aggcagatag aatgatttat tgtcagggaa tacccattca tctttatcaa
     6181 cgccgaataa accaccttgt tgagcatcca gtgcctttaa tttgcatgtt ttgggtattg
     6241 attcatgtag ttcctgcctt ttctctgttt cctgaagtat ttcctttggt ggcatgataa
     6301 gtggcatgat aactgcgggg ggaatcatca gagcatgtaa catacttacg aataaaagcc
     6361 atcgacatac ttgatcctcg atatcattgg tgatgctata tgctatccct gcattgaaaa
     6421 aaaagaatat catatagaca caaatatatt gtggctgaat gacaattaac tttatcaggt
     6481 agtagaagaa attgtaggtc tcgtagagga aaaacattca taccacctat aacactatta
     6541 agatagcctg ccagatatcc atttcagaca ggctatatac agggttcaat tcattctatg
     6601 aaaggtgtat agggattgct gatgtttgtg tcgatcaaaa atctaatatt aatagatgaa
     6661 aactaatata catatttcca gacggatgga gggactttat cgtagagttt attcattgtg
     6721 agttccgcaa gcctgtgatc ggcggcggca ttaaacgact caagctcaga aggggggagg
     6781 cagttttggt ttttttcaat aatcttttca agtgtgtcga gtgttgagca acgtcttaac
     6841 tgaaagagcc agtcttgttt tgttttcatt gtacaattcc tgaagaacat ccttgcccta
     6901 taatagctac aacgatgttt gatttctact gctgggttta ttgacgggag aacgggcaga
     6961 agaaaagccg ctgccgcgcc cccgttctta tcggaagtaa atattataat tagtaattaa
     7021 gacaagaacg aaaaacaaaa gcggaaacac atttttaatg ggggggactg cgttcgccag
     7081 ccccattgta cttacaaaac tgaaagcagc cagtaatagt gaaaacatac cgaaaaaaca
     7141 cgtctcaata acattgcccg tgggaaaaga tattccgata gatttagcca gggaagacac
     7201 tattccgaaa gagttagcca cggaagacac ggtagttaaa acgacaaaga aattgttaac
     7261 agggaaaaca gagtcgtttc tggcaattgt gctatcttta tcctttttta aaataataaa
     7321 tttcatttat ccatccccct aaagcaacgt ctaataatct atgttatgcg catcaatgag
     7381 tgcacgtaga tgttctaact ctttctcttg catctggcat agatgataga gattatcaca
     7441 tttcgtgcga tattcagagc attcagaaag aagactggct gttctcaaat gtaacatcag
     7501 gattatcact aaaaaaacta atacactaac agcgaaaagc aacaaaccat tcaccataaa
     7561 atctgtactc tccacgccga gaaacgaaac atgagaatta tcatcaaaat aaacggatat
     7621 ttcaaaggat aacgccctcc agagaaaagg agggctgtag aatcatttct cgatgagaaa
     7681 ctcatcaagg gaacgacctc ctgccagtgc ttcagcaatc gggtttggcg ctcgcccacg
     7741 accagacttg gctattaact gaagcaattc ctgacctgaa aagccatttc tgctttgctt
     7801 tcacttctta gcccttgccg gaaaacccag cttccctgtt ttcttatcct caaggacaag
     7861 aaccgcatca tacgttccac cgcttttctt tttaaatccc ttaatcaaat cggtttttcc
     7921 tgatttaata agtttttcgg cctgtgcctg ggtgattttc ttgccagaaa actcattcca
     7981 gattttaaat tcacatcctg tacaggaata gctttttggc ctgataacaa tctgtttacc
     8041 acaggaaggg caaggagagg acaggcgttg tgactgacca gacggcgcgc tgggggaaac
     8101 ttttatttca gcactgttcg cattactgat catcggtatc agatcattgt acagttcgtc
     8161 aacgaactgt tcgatggtca tttctccgtt ttcaatgagt gtctgttttt ccgcccataa
     8221 tgccgtcata tctggattaa cagctatatc cggcagagca tcaataagcg catacccggt
     8281 atctgtcgga ataagttttc ctttttccag ggtgatatag ttccgttttt tcagtgtttc
     8341 caatatggaa gcccgcgtag ctggcgtacc aataccgcca tgttcgtctt ttttatcccg
     8401 atctttatcc ttcagtaact ttttaatcac tggatcagtg acaaaatctg cgacacgaac
     8461 aagcgcggca agtaaagtgg cttcggtgaa taatggcggt ggcgttgttt tcttctcatt
     8521 cacaaccact tcttttgtcg tcactgtttc tcctgtgcga attttacaga gaagatcaaa
     8581 agcggaatca tcattttcga cttctgcttc atcgtccccg gcattttcca caccaagaaa
     8641 cgcttcaaat ccgctgtctg tagttttcct ggcacgggca taaaacgact catcaccaca
     8701 ctgaatggcg acggatactt cctgatatgc cttttcaggc atgaactgga caagatagtg
     8761 ttgtgcaatt gccaggtaaa cgttgcgttc gtcggtgctc agtgcgttta cgtccggtac
     8821 gctaacggtt gggatgatgg ctgtatgcgc tgttactttt gcgctgttaa atgccttgct
     8881 tttacgtgtc gtatcaatat ccattggctg atcaaacacc gacttcaggg catcgattat
     8941 ttgtggtgct tcgctgaatt gttcgtctga aagatatgaa caatctgaac ggttgtaagt
     9001 aattgctttg tatttttcgc gtagctgctg tgtgatatcc agcgtttttt gagcagtcat
     9061 tttgaatttt ttgttcatgt actgctgaag tcgtaccagg ttaaagggca acggtgcagc
     9121 cgttttttta tcatctgttg ctgccgcttc aactgtggct ggttttcctg caagtgatgt
     9181 tgctgttcca ttcgcccagg ttttatcaag caatttgcgg tctgtcagcg gtgcgaactc
     9241 accaggtttc cagttcgccc tgataacatc agcgccgcgt tgaaaatgtc cggtcatggt
     9301 gtagtagaag ctggatttat gatttttatt ggcacgggtg cgatttacaa tcaggccaag
     9361 cacgggtgtc tggacccgtc cgacagacaa aacaccttta tagcctttag tttttgccgg
     9421 gattgtgtaa gcacgcgtca tggataaacc gtatatcgcg tcggctacag aacgcgccag
     9481 agccttgaga tagagtcctc tgaagtcacg gttatttttg ggatttgcca gcgccttttt
     9541 cactgccgga agggtgttgt cgttgatcag aacgcgctta acgggttttg tgttgcctgc
     9601 atattccagg acctcatcga caagtagctg tccttcgtca tcaggatcgc cagcatgaat
     9661 aatttcggtc acatcagcac gcctgataag ctcaataatc gttttaacct gtttttcggc
     9721 gctttctaca ggctggtatt ttacgggata aagacgcaaa gggagtgttt caatcttcca
     9781 ctctttgtat tcaggattat agttttcggg gggctgtgat tcgataatat gaccaaaaca
     9841 gttagtcaca atagtattat cactttcaaa ccagccatca tgacgggtaa aattgccccc
     9901 aagcgcctta acaatatcat ttgctactgc gggtttttct gcgataaaaa gtttcatctt
     9961 attatccttt aaataactaa tgcactttca atagatggtt gtcttttgaa taaagagaaa
    10021 tcggtggtta atcgttcaga ctggatcgtg tgaagcaaga tgctttcttc tcttgatatg
    10081 aggaagttac cccactcgaa ctcctcccac atatcatgac tgtcttcacc tggtcttttt
    10141 aaaaatgatt ccattttggg ctggattatt gctggaggct taccttcata agctgaatgc
    10201 accctgtcac tccacttatc aaaagaacca tttaagagaa tttcctgtga taccatatca
    10261 aattcattat cttcaatata agtattaacc tgatgatgaa ttaatcccat atcttcaata
    10321 tcccaggtat atctggtagt gaaccatctt agttttttat ctgtatgcaa aagaagtcca
    10381 ttaactgcct ttctgctttt ccgcattcca acccacaaac atttgttagg ccacggcctc
    10441 cctacagaca atgattcgcc atcaattatt aaatcaagtt caggtatgga gatggatttt
    10501 agagtgcatt tgttcgccat gcgatgaaaa agtttaggga caatatgtag gtacatatct
    10561 atttccttta tctttatcgt agtcaaagaa gttctgattt gttgacctca tggtaaacaa
    10621 gttcaccatt gcataagaca aacagataag aaaactttct ttccttgtca ttcagccagt
    10681 ttttgtactc agaaagaata aacgcccagt catcggtgct gtaatgagat tcgatgacac
    10741 cagagtaaca ccgtttgccg tcatcatctt tttcaccagt gaatacaata ccctctcctg
    10801 gaatttctat ataatgacca ctgttccatt cagggaatgg aggaatatca actgacactc
    10861 cccccatttt ataatcaata actctcgata caccaattat tatcgtcacc attgatttta
    10921 atctctaatt taaaaagaag cctttaatat tgcacaaaga taaaaagaaa gagtcattag
    10981 tatcattaat aaagcgatga taaccatctc aactgtttca gatatattaa attttctgtg
    11041 tgcagttgtt aataaaaatt gacccgttgc cacccagaaa acaaagaaag aaaccataaa
    11101 tacaggattg tcgagtaagt cctgtaaaga ataaaaccag tgaccagcag gatatggcaa
    11161 accaaaagaa gaatgcattt ctttccccca ttatgatttt gaagtattca gcattagtat
    11221 tcatcttcat aaggtagcca atcgtaatcg tttgagttcc agtcaattgg aagaaatggc
    11281 tctgtttgtt tatggcagga gaggcaaaca taaatatttt tctctttatg aaaaatcatc
    11341 gacttacatt tatcacagtc gcaccaaccc tcgactggtt cagggcattc aggtaattct
    11401 gtctcctgat gtttaccata tttttcccat tcctcggcat cctgataacc tccccatact
    11461 gcttgtgtaa aactatcgat atagtcacct gactcatttt tatcaaaatt aaatttatat
    11521 acttcgccat ttagttcaaa tgacatatca aggtcaggta gtaattgctt ttcgaagcgt
    11581 tgttgtaact ctttcttttc tttgggtgat aaagccataa aaacctctat cagttagtta
    11641 tgtttgtatc gccattccca cggtggaacc ggatttgcat cggcccacaa accgcgtttt
    11701 tgttcctgcg cttcccgttg cagagccggt aatgactcat ccacgttgta acgttcatat
    11761 acccatgcag cgccagactg aaccataaag cggctggcat ccgtgccgtt cgtcgtgaac
    11821 acatgaccga taatgcgtcc gtagcggtct ttatgtgaat aagagactgt cacctgttta
    11881 cctgcaacca gagttttaag ctgactagtt gaccagcgtc caaaaggttg tttcttttca
    11941 ggggcatcga tgtttatcaa tcggactcgg atcggaactt catacacaac aatttttgcg
    12001 ggtagtgttt taatttctat ggtgtcgcca tcgagaacgc gaataacttt cccctgaaga
    12061 attttctggt taaatgtata aggtattcca cctgcatttg cagactgaaa agcggtaagc
    12121 agataaataa atattgttaa taagattttc ataaaagatt aacgttaatt tgcctgtgca
    12181 atggatagag cgttattctt attttcttcc tgccgcttca tcgaaaaatg atgattttcc
    12241 tactgctgcg gcatcatact gaaatgaata tccaaaatca tcgtcatgct caaaacctgt
    12301 attttctcca ttttttagtc tggaaagaat aatcttaagt tgactgatta tatcttccct
    12361 ttcactatga cgagtgcctt gtaagagaat agttacttgt tgcaaaggat atgcatgttc
    12421 aatccatgcc tggctaggtc cagagtgcgt tgtatataaa tttttttgtg ccgaaatatt
    12481 attcagaaaa cagtttaagt catcattgga ataaatttct ttaatattgt tgatgttgga
    12541 tgttagtttt acaagttgga atggtggaga atttttgtct tcattgttgt ctcctttatc
    12601 gtattctgga aacacaatta acaaaggtgc accattagtt ctcttcataa aacgggttac
    12661 tagccgccca atatatttat tggacatatg ctgttcaatg tccaggacag catattcctc
    12721 tgctgggaac attttgtttt tttcgatgga tttaattttt ctggtaagat gaatgtttct
    12781 gataagcaaa cctactatca ggaagtaaat aaatatccag aataaaaaat cactctgtag
    12841 ttgttctatc ataagtgaat cctctaattt gttaatggac aaatcattac ccgttcatca
    12901 atgtttgtag gaagttcgcc aggagtaggg acgcgttctc ctcgctgggc ggcttgtgcc
    12961 agtgttcgta gcagcaacac gctggcatga aatatggcct gttcttttgt ggctgcgctt
    13021 gtttccaggt cgaaatcact cagagtcaca acgacttgcc cgtttctgtt ctcgaccgtt
    13081 gccgggtagg gaacaaaata gggagcgatt ttgctggttt gcttgcgtcg ggcatcgagt
    13141 ttactgacaa gttgataagc ccgaaggtgt gtgtatcgtt tcaacatgtt cagggagcga
    13201 tggcctgaaa tagcggcaac ctcaatgacg ttcaatgtac caagttcgaa aaatcggctg
    13261 atagcctcat gacggaggtc atgaaaatgc aagttttcta tcttcagttc ctgaagtgct
    13321 gttcgccagg cacttttgaa tcccgaagag gtataagaaa aaatattacc gttaagctgt
    13381 gtcggcagca tttgcaggta attacgggct ttcctggata gcggaacatc gcgtggagca
    13441 ccgtttttgg ttgttggaag atgagctacc ccatgttgca gatcgacgtg ttcccaacgc
    13501 aatgacaaga tttcaccttg tcgcattgct gtttccagcg ccagatgaaa aatgacgtaa
    13561 agagcctgat ttttttcttt gaaataccgc gaaagtcggc gttcttctcc tgaagtcagg
    13621 cgacgatcgc gcccgctact gattttgggt tttctgacca gttcgacagg attcatgcgg
    13681 catgttcccc attccacacg ggcgatattg aataatgatg acaacagggc aagttcaaga
    13741 cggacggtgt ttccggttat ttgacgcccc gttcgtgggt taatttgcgc caaccgttga
    13801 tcccgataat tggcaatatc aacagtggta atgtcatcca tatagcgttc agcaataggg
    13861 tgacgtttaa tcacatttac gcgataaaac tcctgtagat ggcctttttt gtggatggat
    13921 actgtcttca ggtatttgtc cagcgcccgg ctgattgtca ttttgcgaat tttgattttc
    13981 tgaaacatat cccctccaga caaaaaaggg gggaaggtag agattcttgc ttcgaaatgc
    14041 aatatc
//
LOCUS       DAZPLM010000102        13973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPLM010000102 DAZPLM010000000
VERSION     DAZPLM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.102"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..230)
                     /locus_tag="ACHVC7_002543"
     CDS             complement(<1..230)
                     /locus_tag="ACHVC7_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116565.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAV"
     gene            265..990
                     /locus_tag="ACHVC7_002544"
     CDS             265..990
                     /locus_tag="ACHVC7_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR2116566.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1483..2229
                     /gene="mipA"
                     /locus_tag="ACHVC7_002545"
     CDS             1483..2229
                     /gene="mipA"
                     /locus_tag="ACHVC7_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR2116567.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2319..3173
                     /gene="yeaE"
                     /locus_tag="ACHVC7_002546"
     CDS             2319..3173
                     /gene="yeaE"
                     /locus_tag="ACHVC7_002546"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR2116568.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3224..4108)
                     /locus_tag="ACHVC7_002547"
     CDS             complement(3224..4108)
                     /locus_tag="ACHVC7_002547"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR2116569.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4192..5187)
                     /gene="gapA"
                     /locus_tag="ACHVC7_002548"
     CDS             complement(4192..5187)
                     /gene="gapA"
                     /locus_tag="ACHVC7_002548"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR2116570.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5529..5942
                     /gene="msrB"
                     /locus_tag="ACHVC7_002549"
     CDS             5529..5942
                     /gene="msrB"
                     /locus_tag="ACHVC7_002549"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR2116571.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5984..6256
                     /locus_tag="ACHVC7_002550"
     CDS             5984..6256
                     /locus_tag="ACHVC7_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR2116572.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6417..7058)
                     /gene="pncA"
                     /locus_tag="ACHVC7_002551"
     CDS             complement(6417..7058)
                     /gene="pncA"
                     /locus_tag="ACHVC7_002551"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR2116573.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7069..8085)
                     /gene="ansA"
                     /locus_tag="ACHVC7_002552"
     CDS             complement(7069..8085)
                     /gene="ansA"
                     /locus_tag="ACHVC7_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR2116574.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8252..10108)
                     /gene="sppA"
                     /locus_tag="ACHVC7_002553"
     CDS             complement(8252..10108)
                     /gene="sppA"
                     /locus_tag="ACHVC7_002553"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR2116575.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10269..10820
                     /locus_tag="ACHVC7_002554"
     CDS             10269..10820
                     /locus_tag="ACHVC7_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR2116576.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10937..11980
                     /gene="selD"
                     /locus_tag="ACHVC7_002555"
     CDS             10937..11980
                     /gene="selD"
                     /locus_tag="ACHVC7_002555"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR2116577.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGHIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11985..13946
                     /gene="topB"
                     /locus_tag="ACHVC7_002556"
     CDS             11985..13946
                     /gene="topB"
                     /locus_tag="ACHVC7_002556"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR2116578.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3584 a         3370 c         3632 g         3387 t
ORIGIN      
        1 acagcggaga tagcaatacc gaaaaccatc cacatatcta tagggctttt atttgtcgat
       61 ttgaccgtag tgtacttttt gactaagtcc gtacctgata gcactgttaa aattaatgat
      121 aatatagcga gggcagtatt tacattaaaa tttagcttag cgaataaaag caacattagt
      181 aaaataaaaa taagtaacgc tatgattaaa aatattcctt tcttgtccat aaataccccg
      241 tcgtattttt aaaaaaggta tcttatggca aggataagta aaaaggaatc tgctaatcac
      301 aataaagtga tggaattaat acattcagat aagctcctaa cacaagaaga tagggaattc
      361 atattcacca attatagggg ggatgggata ggggctacag gtgccttctt cacacctgaa
      421 attttagcat gggattttgt acttgatgct gactgtactg gtgactgtat tgaactatgt
      481 gctggtatcg gtcgcctcag ctactaccag tacttaagga ataaaccaac gcatattaca
      541 tgcgtggaac tcaatcctga gtacgtaatg atcgggaaga gggtactacc agaggcagaa
      601 tggatcactg gtaacgcact acagtactca ccagaccgtt tctatgatgt tgcctatggt
      661 aatccaccct ttgggaagat cagtacctca aaagcgtata caggccgtta taagggtagt
      721 gaatttgaat acaaggtgat tgaacatgca agtacctttg cgtcctatgg tgcatggata
      781 gtaccgcaag gttccgcagg ctttgtctac agtggggtac attgttatga gcgtagagaa
      841 tcatgtaagt accagaagtt tgtgaaggat actggctatg agctacatcc aggtgtaggc
      901 atcgatacaa gtatctacag ggatgaatgg aatggtacta aggtgatatg cgaatcggtg
      961 ttagtagatg atgacggtac aaatctgtaa ctaaattagt taagaaatca taaagatagt
     1021 agtacttgca aatgttccca ttgatgaagt tatgcgtcct ttcgtttttt agcacaggtt
     1081 aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac gacgtctctt
     1141 gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt gcccggtgat
     1201 acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg ctttcttaat
     1261 ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa agacaaggct
     1321 cgccagtaac gctaaattca tttggctgta agcgcggtgt catccgcgtc aggaaaatta
     1381 aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa
     1441 actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa actcaaactt
     1501 ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt
     1561 tccctgggcg caggcgtcgg tgtcgttgaa cacccatata aagattacga taccgatgtt
     1621 tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg cttaggtggt
     1681 ggttactacc tgtggaatga cgcaacggac aaactttcaa ttaccgctta ttggtcgccg
     1741 ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga tgaccgtaag
     1801 agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta cctgcgtacc
     1861 accctggctg gcgataccct ggataacagc aacggcattg tctgggatat ggcctggttg
     1921 tatcgttaca ccaacggcgg cctgacagtg accccgggta tcggtgtgca gtggaacagc
     1981 gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg
     2041 cgtggctata acccgaacga cagctggagc ccttacctgg agctgagcgc cagctacaac
     2101 ttcctcggcg actggagtgt ttacggtacc gcgcgctata cccgtctgtc tgatgaagtt
     2161 actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg gatcacctac
     2221 aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc gtcacgccct
     2281 gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa atgattcaat
     2341 ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg ggcgaagatg
     2401 ccagtcagcg caaaacagaa gttgctgcac tacgcgcggg cattgagctc ggtttaaccc
     2461 tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt ggggaagcat
     2521 taaccggtct gcgtgataac gtctttctcg tctctaaagt ctatccgtgg aatgctggcg
     2581 ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact gattatctcg
     2641 atctctactt attacacagg tctggcagtt tcgcttttga agagactgtt gcagcgatgg
     2701 aaaaattgat cgcccaggga aaaatccgcc gctggggcgt ttctaacctt gattatgctg
     2761 atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat caggtgcttt
     2821 accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag caacagcaga
     2881 tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat ggactgttaa
     2941 aaaacgcggt agtcaacgaa attgcacatg ctcacaatat cagctcggca caagtattgt
     3001 tggcgtgggt aatcagccat cagggtgtga tggcgattcc aaaagcggcc acgattgccc
     3061 atgttcaaca gaatgcggct gtgcttgagg tcgaactttc gtcagcggaa ttaaccatgc
     3121 tggataaagc gtatccggca ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat
     3181 acgtgccgga tgcatcgcac ccggcacaca acattaacgc aaattaacgt ttcgcaacgc
     3241 gaatggattg cgccagatgt gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt
     3301 aagccgtttc tacacaaaca aatgttttgt agccatcatc cggcatatcg cccatgctaa
     3361 ttgaaagcgc cggtcccggg ttccagccta caacgttcag atgatgctgg tggcctacgg
     3421 cgataatacg attcagcgct tcatcattaa tcacgctgca atcttgcgga ttcagataca
     3481 cgcgatcggt acggtcaggg aaggtctgaa taccgtcggt cagtacatct tctttcgcgt
     3541 cattcacttt atcaatgaag cgatcgccca gcccactgac gcttacctta gcgatatcac
     3601 ccacgttaaa gtaggtatgc agggcagagg tggtttcaaa ttcgccatgt gattcaagat
     3661 cgatttcaca ggttttaccc acgcggaaat gcgctaacag cgtaaagtcg tgcggacaga
     3721 attttttcgt ctcttcgctt tgcgtcaatt caaaagtcag cgctacgcca tcagcatctt
     3781 cattatgtga tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt
     3841 gttgtgccgc cggaccaaac cacggccagc aaaccggtac gccaccgcga atagcgacgc
     3901 catttttgaa cggtgtgttg ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc
     3961 acgagagaag gtgtgcgccc tgtaatgcaa aagaggcttt tacttgggga tgatcgacca
     4021 caatgaggtc cagttcatcc agtttacgac gggagaggac aggggagatt tgttcgatga
     4081 ccggaagggc aaaaattttc ttaatcatga cgcagtcctt taacttcatt ttatcaggta
     4141 aaaaaaagag cgaccgaagt cgctcttttt agatcacagt ttcatctcaa cttatttgga
     4201 gatgtgagcg atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga
     4261 taccagtttc acgaagttgt cgttcagagc gataccagct ttagcatcga acacggaagt
     4321 gcaaacttcg ccgttgaaat cggtagatac tacgtcatct tcggtgtagc ccagaacgcc
     4381 tttcatttcg ccttcagcag cggctttaac ggcagctttg atctgctcgt aagttgcagc
     4441 tttttccaga cgaacggtca ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc
     4501 cataccagtc agtttgccat tcagttctgg cagtacttta cctacagctt tagcagcacc
     4561 ggtagaggac gggatgatgt tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga
     4621 cgggccatca acggttttct gagtagcggt agtagcgtga acggtggtca tcagaccttc
     4681 gatgatgccg aagttatcgt tgataacttt agccagcgga gccaggcagt tggtggtgca
     4741 ggaagcgttg gaaacgatgt cttggccagc atatttgtcg aagttagcgc ctttaacgaa
     4801 catcggagtg ttgtctttag acggaccagt cataaccact ttcttcgcac cagcggtgat
     4861 gtgtttacga gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga caacgtcaac
     4921 accaacttcg tcccatttca ggttagccgg atcacgttca gcggtaacac ggattttttt
     4981 accgttaacg atcagatgac cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt
     5041 ggagtcatat ttcagcatgt atgccatgta atcagcgtct aacaggtcgt tgattgcaac
     5101 gatctcgatg tcagaacgtt tctgagcagc acggaaaaca atgcgaccga tacggccaaa
     5161 accgttgata cctactttga tagtcatata ttccaccagc tatttgttag tgaataaaag
     5221 gttgcctgta aaattacaaa aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc
     5281 gacaaatcaa tcctgtgcct aagcattacg cgactgactc gcctcactct tcctttgggc
     5341 ttgaaaccac atggggtcgg cgccccgaat tttaaagggc aattacgata aaaatgtgat
     5401 tttcatcacg atttaggtgc agccatttcg tcgagatcaa gtttaggaca aaattttgcc
     5461 agttggtgtt aatgttttgt tagaatcagt cacgtaatgt gagcacgatt aaagtgagat
     5521 gtgagcaaat ggctaataaa ccttcggcag aagaactgaa aaaaaatttg tccgagatgc
     5581 agttttacgt gacgcagaat catgggacag agccgccatt tacgggtcgt ttactgcata
     5641 acaagcgtga cggcgtatat cactgtttga tctgcgatgc cccgctgttt cattcccaaa
     5701 ccaagtatga ttccggctgt ggttggccca gtttctacga accggtgagt gaagaatcca
     5761 ttcgttatat caaagacttg tcacatggaa tgcagcgcat agaaattcgt tgcggtaact
     5821 gtgatgccca tctggggcat gtcttccctg acgggccgca gccaacgggc gaacgttatt
     5881 gtgttaactc tgcttcttta cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt
     5941 gaaaaaacga ttcagcaact tattccacag gagcggaaaa attatgaatc ttgatgacat
     6001 tatcaacagc atgacgcctg aggtatacca gcgtttgtcg accgccgttg aactggggaa
     6061 atggcccgat ggcgttgcgt tgaccgagga acaaaaagcg aactgcctgc aactggtgat
     6121 gctgtggcaa gctcgccata ataccgaagc acagcatatg acaattgaca caaatggtca
     6181 gatggtcatg aagagcaaac agcagttaaa agaagatttt ggtatctcag caaagcctat
     6241 tgctatgttt aagtgaatga gttatctagt atttaagcct gttattaagg gagggcaaat
     6301 atacttgtta ggacgtcgtg ttttagctgc aagtaggtta aatgcaacac tgacaagtat
     6361 tatctggcct acaatgtcat tgaggaaatg taggccagag atgaattcaa ctaatattac
     6421 ccctgtgtct cttcccagtc tgccagcgta tatagcgttg ccccagctgc tgacatctcc
     6481 ataaacgcgt gcgcactgtc ctggggctgg atattcacgc cacgacaacc atcggtaatc
     6541 acatttacct tataacctaa ctgtaacgcg tccagcacgg taaacttcac gcaatagtca
     6601 gtagccaggc ccataacgat caattcatcg atttcatgat cgcgtaacca gtcatcgagc
     6661 gaggttttct gccgacggcc gttatcaaaa aaggcactgt aactgtcaac taaaggattt
     6721 tcgcctttat ggaacaccgc tgcgatcgct ttttggttca gtaacggatg taattgtgcg
     6781 ccttcactgt tctgcacaca gtgatctggc cagaaggttt gtggcaaacc gtcgagttgg
     6841 cctggcgtat aaggctctac accgtgctga ctggcaaaac tgccgtgatt cgccgggtgc
     6901 cagtcctgac tggcgataac cgcttcaccg cgcgactggc accagtcaat caggcggtta
     6961 gcgacatcca ccgtactgtc accttccggc acggcgagcg cgccaccagc acagaaatca
     7021 ttttgtaaat cgaccagtaa cagggcgcga gggggcatta cagtctcctt aatcatccgg
     7081 cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga atggtttcag tatccagttc
     7141 ctggctcagc aggtaatgca gtttggttag cgtggcttct acagtcatat ctgcaccgcc
     7201 aataacgccg gcatgggcga gggcgttacc ggtggcataa ccacccatat tcactttacc
     7261 ggacatacat tgtgtcaggt tgaccaccac aataccgcga tcgctggctt cttgtaattc
     7321 ctgcaggaag gctttgtttt gtggcgcatt acccacgcca taggagcgca gaatcaatgc
     7381 tttcaccggt tggcgcagaa aattgcgcac gacgtcagca gaaatccctg gataaatcgt
     7441 cactacgcca attggttgtg gcgtgattgg atgaacgatt aattcccctt caccgtgcgg
     7501 ggcgggtggc gtattcaaac gacgaatatg gataccagct tccagtaacg gaggaaggtt
     7561 tggagaggca aacgcatcaa aaccatcggc atgggctttg gtagtgcggt tgccgcgata
     7621 caatcggtta ttgaaaaaga gcgttacttc gttgatcgga taattcgccg caacgtacaa
     7681 cgcattcagc agattaattt gtccgtcaga gcgtaactca gccagcggga tttgtgaccc
     7741 tgtcacaatg accggtttac cgagattctc gagcatgaac gacagcgcag aggcagtata
     7801 cgccatcgtg tcggtgccgt gcaggatgac aaaaccatca tagtcgtcat agtgcgcttt
     7861 aatatcttca gcaatatgct gccagtcttc tggcgtcata tcagaagaat ccatcagcgg
     7921 cgtatattca tgaatggtga aatctggcat ctccgggcga tggaattccg gcatcagcgc
     7981 cagttggcgt tgtagatgac ctgacaccgg aatataaccc tgctcggaac gctgcatccc
     8041 gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt tgcatgatat tgatatgaat
     8101 gatatcgaaa gagcaaaagt atatacgtga ggcaggagga gcgcacctga tactatgcgg
     8161 ataaggaaat atggggcgag acagcgggaa gaggattcgt cgacgtaaaa aataatgtgg
     8221 ccgacgatct gtcggccact cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa
     8281 acgcataacg gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg
     8341 tagaggctac cgaggcaagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg
     8401 cccggacaga accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt
     8461 agtattccag atgccactgt ttcacttttg ccagctctgc tgctttggcg accgcatcat
     8521 cgaaatcccc gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt
     8581 ggccctgggc gattttatca atctgctccg gcgtcgaatg acgcgcatca gcaaccagcg
     8641 tgataaagcg tttatagcca ttctcaatgc ttaattgcat catctgctgc gcttccggcg
     8701 gcagtgccct ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa
     8761 caccaatcga atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac
     8821 cggtcagggt gctggggtta gccacaatgt aattagctgg cgtggaaatc cagtaaccac
     8881 cagatgccgc catgccgccc atcgatacaa ccacaggctt acccgctgcc cgggctgctg
     8941 ccagttcagc gcgaatcact tcagacgcgg taacgctgcc gcctgggcta ttaacacgca
     9001 ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat
     9061 caccgccaac attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga
     9121 caccgatgct gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg
     9181 cgcgataatt tttatcagtc ttactccagc cgaactcttt ggttagagct ttttcgattt
     9241 cggcactcga tgccagtgca tcgaccagct tgttttccag tgcatatttc gcggtatcgc
     9301 caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag
     9361 ggatctgccg gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc
     9421 ggctgtcagc ttcgcgggct gccggtgaca tgtcatcacg aataaacggt tcaacggcag
     9481 atttatacgt acccacgcgg aacacatggg tggaaacttt cagcttatcc agcaacgatt
     9541 tgtagtacag accgttggta gcaaaaccgt gcaaatccac cacgccttgc ggtgacagcc
     9601 aaattttatt ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg
     9661 cataaaccgg tttcccgctg tcacgaaact ctttcagggc tttgccgatg tactgcatag
     9721 acggctggtc gccgcctgcg aagtttttca gatccatcac gataccggtg atattgcggt
     9781 cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgcagacgat
     9841 cggaactggc accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtcgggtt
     9901 tatcgacgat cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg
     9961 aatcaccacc actgacctgc atccagatcc ccacaccaac cagtacgagg aaaataaaga
    10021 acaggttaag taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc
    10081 cggcaataaa tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtcac
    10141 aggcgcaata gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg
    10201 actgtgctgt aacaaaatcc actctcgtgt taattttgtg aataaatatc acgacaggag
    10261 ttaatcaaat ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg
    10321 aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg
    10381 accataagtc catgcaaccg tggcattttt ttgtgattga aggggaaggg cgcgagcgtt
    10441 tcagcgccgt actggaacag ggggcgattg ctgccggtag tgatgacaaa gctatcgaca
    10501 aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac ggtggtggcg aaatgcgaag
    10561 aaaatcataa agtcccgcga tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga
    10621 tgcaaatggc agccgttgcc cagggatttg gcggcatctg gcgcagtggc gcattaactg
    10681 aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc
    10741 tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc
    10801 cgtttgtaac ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca
    10861 ggaattcaga cactctcact tatcacttca cggaatgagg gttaccatac ccggattgca
    10921 ttgacaggag atgtccatga gcgagaactc gattcgtttg acccaataca gccacggagc
    10981 tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc
    11041 gaagtttgtt gatccgaatt tgcttgtggg taatgagacc cgcgacgatg cggcggtgta
    11101 cgatctgggc aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga
    11161 taatcctttc gattttggcc acattgcggc gactaacgcc atcagcgata tcttcgcgat
    11221 ggggggcaaa ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc
    11281 ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc
    11341 gctggctggc ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg
    11401 gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt
    11461 cctgacgaaa ccgctgggga tcggcgttct taccacggct gagaaaaaat cactgctgaa
    11521 accagaacat cagggactag cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc
    11581 ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt acgggttttg gtctgctggg
    11641 ccatttgagc gaaatgtgtc agggcgcagg tgtgcaggcg cgcgttgact atgacgcgat
    11701 cccaaaattg ccgggggttg aagagtacat taagttgggc gcagtgcctg gcggcaccga
    11761 acgtaacttt gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgattt
    11821 gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa
    11881 tgaggtcaaa gctacagccg ccgagtttgg cattgaactg acggcaattg gcgaactggt
    11941 gccagcgcgc ggcggtcgtg ccatggttga gattcgttaa ttcaatgcgg ttgtttattg
    12001 ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct gcccaaaccg caccggaaag
    12061 gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc
    12121 ttgagcaggc gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt
    12181 tgccgattgt cccggaaaag tggcaattac agccccgacc ctccgtgacc aaacaactta
    12241 acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt tcacgccggg gacccggatc
    12301 gtgaagggca actgctggtg gatgaagtgc tggactatct gcaactggca ccggaaaagc
    12361 gccagcaggt gcagcgttgc ttgataaacg acctgaaccc gcaggcggtt gagcgggcgc
    12421 tcgaccgtct tcggtccaac agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag
    12481 cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc gtataccatt ctcggtcgca
    12541 atgccggtta tcagggcgta ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg
    12601 tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag
    12661 cacatatcgt gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt
    12721 gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg
    12781 ttaaccgcat tagtggtcaa ccagcaattg tcaccagcta taacgataaa cgggaatcag
    12841 aatccgcgcc gctgcctttt tcgctttcgg cgttgcagat tgaagcggca aaacgctttg
    12901 gtctgagcgc gcagaacgtg cttgatatct gccagaagct gtacgaaacg cacaagctaa
    12961 tcacttatcc gcgttccgat tgtcgctatt tgccagaaga acattttgcc ggacgccacg
    13021 cggtgatgaa tgccatcagc gttcatgcac cagatctgtt gctgcagcca gtggtagatc
    13081 cagatatacg caaccgctgt tgggatgaca aaaaggtcga tgcacaccac gccatcatcc
    13141 cgaccgcacg gagttcagcc atcaacctga cggagaacga agcgaaggtc tataacctga
    13201 ttgcccgtca gtacttgatg cagttctgcc cggatgtggt gttccgcaag tgtgttatcg
    13261 aactggacat tgccaaaggc aaatttgtcg ctaaagcgcg ttttcttgct gaagcaggct
    13321 ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa cgacggtacg ccattgcctg
    13381 tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc
    13441 agccgccgcg ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct
    13501 ttgtgcagga caaagatctg aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag
    13561 caacccgtgc cgggattatt gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc
    13621 gctatatcca ctctaccgac gccggaaaag cgttattcca ttcgctgcca gaaatggcga
    13681 cgcgaccgga catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt
    13741 gtcgctatca ggactttatg cagccgctgg tggggacgct atatcagctt attgatcaag
    13801 ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg
    13861 ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg
    13921 aagtcggaag cggggcgata gcgtaagcga gtgaatcttt cgtgctattc gag
//
LOCUS       DAZPLM010000103        13962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPLM010000103 DAZPLM010000000
VERSION     DAZPLM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.103"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            73..2214
                     /gene="ldcC"
                     /locus_tag="ACHVC7_002557"
     CDS             73..2214
                     /gene="ldcC"
                     /locus_tag="ACHVC7_002557"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR2116579.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2271..2660
                     /locus_tag="ACHVC7_002558"
     CDS             2271..2660
                     /locus_tag="ACHVC7_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2116580.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2725..4023
                     /gene="tilS"
                     /locus_tag="ACHVC7_002559"
     CDS             2725..4023
                     /gene="tilS"
                     /locus_tag="ACHVC7_002559"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR2116581.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHVC7_002560"
     CDS             complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHVC7_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR2116582.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4319..4519)
                     /locus_tag="ACHVC7_002561"
     CDS             complement(4319..4519)
                     /locus_tag="ACHVC7_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR2116583.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4685..5230
                     /locus_tag="ACHVC7_002562"
     CDS             4685..5230
                     /locus_tag="ACHVC7_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR2116584.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5227..5588
                     /gene="arfB"
                     /locus_tag="ACHVC7_002563"
                     /pseudo
     CDS             5227..5588
                     /gene="arfB"
                     /locus_tag="ACHVC7_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHVC7_002564"
                     /gene_synonym="cutF"
     CDS             5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHVC7_002564"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR2116585.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6527..7351)
                     /locus_tag="ACHVC7_002565"
     CDS             complement(6527..7351)
                     /locus_tag="ACHVC7_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR2116586.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHVC7_002566"
     CDS             complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHVC7_002566"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR2116587.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHVC7_002567"
     CDS             complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHVC7_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR2116588.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHVC7_002568"
     CDS             complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHVC7_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR2116589.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHVC7_002569"
     CDS             complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHVC7_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR2116590.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11314..11967)
                     /locus_tag="ACHVC7_002570"
     CDS             complement(11314..11967)
                     /locus_tag="ACHVC7_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR2116591.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHVC7_002571"
     CDS             complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHVC7_002571"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR2116592.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHVC7_002572"
     CDS             13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHVC7_002572"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR2116593.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3389 a         3535 c         3712 g         3326 t
ORIGIN      
        1 cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc
       61 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
      121 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
      181 caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg tggcgtgatt
      241 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
      301 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
      361 cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt
      421 atgcgtcagt acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg
      481 tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
      541 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
      601 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
      661 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
      721 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
      781 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
      841 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
      901 cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
      961 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
     1021 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
     1081 acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga
     1141 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
     1201 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
     1261 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
     1321 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
     1381 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
     1441 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
     1501 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg
     1561 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
     1621 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
     1681 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
     1741 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
     1801 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
     1861 attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg
     1921 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
     1981 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
     2041 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
     2101 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
     2161 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
     2221 agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
     2281 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
     2341 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
     2401 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
     2461 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
     2521 atgatatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
     2581 gtgtcgatcc atacacgcaa aaacgcttca cgttctttaa cgatccggac gggctgccgt
     2641 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
     2701 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
     2761 cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
     2821 gtgcagtggc ggacggaaaa tccgggcgtc actctacgcg ctatccatgt gcatcatggt
     2881 ttaagtgcta atgccgatgc ctgggttagg cattgcgaga acatctgcca acagtggcag
     2941 gtgccgctgg tggtcgagcg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag
     3001 gcgcggcagg cgcgttatca ggcatttgct cgtaccctgt tacccggtga agtgctggtc
     3061 accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc
     3121 ggccctgccg ggctttcggc tatgggggaa gtttcggaat ttgccggaac acggcttatc
     3181 cgcccgttgc ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta
     3241 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
     3301 cgtgtagtgc cgttattgca gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc
     3361 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
     3421 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
     3481 cgtcgcgcgg cgattatccg ccgctggctg gcagggcaga atgcaccgat gccttcccgc
     3541 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
     3601 ttacgttttg gcgcgtttga aatacggcgc tatcagtcgc aactgtggtg gattaaatcc
     3661 gtcactgggc aaagcgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
     3721 ccggcggggc tgggaagtgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
     3781 gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgtaac
     3841 ggcggacgta agctaaagaa aatctggcaa gagctgggcg tgccgccgtg gctacgtgac
     3901 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
     3961 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
     4021 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc
     4081 gcttaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgcag
     4141 ctcgcgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
     4201 atcactggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
     4261 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgcatc
     4321 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
     4381 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
     4441 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
     4501 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
     4561 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
     4621 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
     4681 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
     4741 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
     4801 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
     4861 tggtttgtgt gccgattatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
     4921 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
     4981 cgcaaaagtg gcgctgttta cctataatag ccgggcggcg caaatctggt ggcagcaaaa
     5041 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
     5101 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
     5161 cgtgatctgg ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca
     5221 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
     5281 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggctattcat
     5341 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
     5401 gccagccatc atttgatcag catgatggcg taattgtcat taaggcacag gaataccgca
     5461 gtcaggaact gaaccgcgaa gcggcgctgg cccggctggt ggcagtgatt aaagatttaa
     5521 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
     5581 tggcatagaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
     5641 gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
     5701 agcctcttta ctctgatggg atgtaataat cgggccgaag tcgatacgct ttctccggcg
     5761 caggctgccg aactaaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgccgat
     5821 tgcgaaggga tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
     5881 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
     5941 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
     6001 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat
     6061 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
     6121 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
     6181 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
     6241 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
     6301 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
     6361 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct
     6421 tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata
     6481 aggcgttcac gacgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc
     6541 caacaaagcg acccgccttg atataaatcc ctggcttcag gtgcccaaca tactgtaact
     6601 gcgtttctgg cttaaacgca gagacatcgc cagtacgcat atgcatcaga tcggcagggc
     6661 taatccagcc tgattgcgcc agcgtaagcg gatgcccggc tttggcaaat gcatcagtga
     6721 caaattccga acaaaaccac gactttttgt ctccttcgcc cacactgctt aactgcgctt
     6781 tcgccaggcc gctgacgcac tgttggcgaa aatcctcgga gaacggattc agtgagcaca
     6841 tctggcgagt caccataaag ggaataaatt cgacaatgcc gcgatagtta taactgctat
     6901 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
     6961 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acgatctgga
     7021 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag atagattgca acgtgactca
     7081 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
     7141 gcaaatcgcc gggtttgaga tcaggtaccg ttatttcttt tattgattgt tcggtgaaag
     7201 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
     7261 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
     7321 gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaagggccct
     7381 gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg
     7441 tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg
     7501 ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca
     7561 gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca
     7621 cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc
     7681 atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat gccgcgttcg
     7741 tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc
     7801 atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag
     7861 tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta
     7921 ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg
     7981 gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat gttagcacca
     8041 gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt
     8101 accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc ttcggtcgcg
     8161 aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga
     8221 tcaccgcgca ccagcagcgc aaccagcggg aagctgctgc cttcaaccgc tttaaccagc
     8281 agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt
     8341 ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc
     8401 ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc
     8461 acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata
     8521 gaaccggtgt cggcttgcac ggcgcggaaa tccagcccca tgcggctgaa gattttgctg
     8581 taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag
     8641 taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg
     8701 tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc
     8761 tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc
     8821 tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct
     8881 tgccacaaat cggctggctg aaccaccggc atcgacacct cgattgcacc ggcgttgttc
     8941 atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag
     9001 gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg
     9061 atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc
     9121 atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa
     9181 agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg
     9241 gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat
     9301 gtagccagac ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgcgggt
     9361 cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca
     9421 aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt
     9481 gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat
     9541 ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt
     9601 tatggcaaac aacctctttc agctctacca gcgacatacc aattgggtta gggcggaaag
     9661 tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag
     9721 tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga
     9781 acgcttccag gccgcgaacg gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc
     9841 cgttggcgct ttttaccaga ccaggctggc gcggaacggc gaatttttct ttatagggcg
     9901 agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc
     9961 agagccgata catacagcct gacgatagca gcctagcgta ccgctggtga cttcgcagct
    10021 atgcagtaat accgcattgg ctttcatttt agaggcgttg atttgcatcc gcttacgcgc
    10081 ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc
    10141 accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg
    10201 cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg
    10261 aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa
    10321 acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa
    10381 tcaggagctt gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt
    10441 gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc
    10501 tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc
    10561 tttgttatct tcacgcgtta cgatcaggtt cacgtacggg gagtctttat cttcgacaaa
    10621 gataccgtct ttcgctggag tcaggccaat ctggctggca taggtggtat tgataactgc
    10681 cagagcgatt tgcgcgtcgt ccagagatcg aggcagctgc ggtgcttcca gctcaacaat
    10741 tttcagattt tttgggttct caacaacatc aagaacggtc ggcagcaggc caacgccatc
    10801 tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg
    10861 atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt
    10921 ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg
    10981 atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc
    11041 tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag
    11101 gccatatttg tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac
    11161 aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc
    11221 cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta
    11281 ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat
    11341 gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt
    11401 catcaccgtc gcgttgtagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc
    11461 ggcaccgact gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc
    11521 cgcattcacc agacccggca acgcttccgg taacagcact ttacggacga tctgcattgg
    11581 cgtggcaccc attgcgcggg aagcttcaat taacccggtt gggatctcca gcagagcgtt
    11641 ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa ttgctgcctg
    11701 caaaccaatc gatgtaccga caataacgcg ggtaaacgga atcatccata ccagcaagat
    11761 aatgaacggg atggaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc
    11821 gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat
    11881 cacaaagcca aaaaatccgg atacgaaggt cattgccagc gtttcccata cgccacgaac
    11941 cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct
    12001 gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga
    12061 tgccgaattt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt
    12121 tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg gtaaactcca
    12181 gacgcagcat cggcacgcag tcagtaaatg gctccgcttg cagacgttcc tggtaatctt
    12241 ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat
    12301 gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca
    12361 cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac
    12421 ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca
    12481 gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa
    12541 tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc
    12601 caagaccaac caatgacagc aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt
    12661 tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa
    12721 aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg
    12781 tggtcaattc ttggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta
    12841 cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc
    12901 cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga
    12961 acactttggt gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt
    13021 cgtggcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc
    13081 cagacgtcta aatcaatcag gtttatgcga agagcacttt cttgcaggtc gaaacatgcg
    13141 atactagcgt cccatgcctt attaaggagc tataaaaggt ggcgaagagc gtacccgcaa
    13201 tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca
    13261 actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg
    13321 cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt
    13381 ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg
    13441 gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc caggtctgcg
    13501 attgccgcaa accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata
    13561 tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg gttgcggcga
    13621 acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg
    13681 cggcagattg ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa
    13741 aaccggcgca atgattaaaa gatgagcggt tgaaataaaa atgcattttt ccgcttgtct
    13801 tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca
    13861 caaacagccg gttcggttga agagaaaaaa tcctgaaatt cagggttgac tctgaaagag
    13921 gaaagcgtaa tatacgccac ctcgcgacag tgagctgaaa gc
//
LOCUS       DAZPLM010000104        13855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPLM010000104 DAZPLM010000000
VERSION     DAZPLM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.104"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHVC7_002573"
                     /pseudo
     CDS             complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHVC7_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHVC7_002574"
     CDS             1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHVC7_002574"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR2116594.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHVC7_002575"
     CDS             1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHVC7_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR2116595.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHVC7_002576"
     CDS             complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHVC7_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR2116596.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4179..5417
                     /locus_tag="ACHVC7_002577"
     CDS             4179..5417
                     /locus_tag="ACHVC7_002577"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR2116597.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHVC7_002578"
     CDS             complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHVC7_002578"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR2116598.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHVC7_002579"
     CDS             complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHVC7_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR2116599.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHVC7_002580"
     CDS             complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHVC7_002580"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR2116600.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7208..8671)
                     /gene="citT"
                     /locus_tag="ACHVC7_002581"
     CDS             complement(7208..8671)
                     /gene="citT"
                     /locus_tag="ACHVC7_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR2116601.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8722..9600)
                     /gene="citG"
                     /locus_tag="ACHVC7_002582"
     CDS             complement(8722..9600)
                     /gene="citG"
                     /locus_tag="ACHVC7_002582"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR2116602.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9575..10126)
                     /gene="citX"
                     /locus_tag="ACHVC7_002583"
     CDS             complement(9575..10126)
                     /gene="citX"
                     /locus_tag="ACHVC7_002583"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR2116603.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10130..11662)
                     /gene="citF"
                     /locus_tag="ACHVC7_002584"
     CDS             complement(10130..11662)
                     /gene="citF"
                     /locus_tag="ACHVC7_002584"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR2116604.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11673..12581)
                     /gene="citE"
                     /locus_tag="ACHVC7_002585"
     CDS             complement(11673..12581)
                     /gene="citE"
                     /locus_tag="ACHVC7_002585"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR2116605.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12578..12874)
                     /gene="citD"
                     /locus_tag="ACHVC7_002586"
     CDS             complement(12578..12874)
                     /gene="citD"
                     /locus_tag="ACHVC7_002586"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR2116606.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12889..>13855)
                     /gene="citC"
                     /locus_tag="ACHVC7_002587"
     CDS             complement(12889..>13855)
                     /gene="citC"
                     /locus_tag="ACHVC7_002587"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR2116607.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3507 c         3688 g         3259 t
ORIGIN      
        1 ataatgatat taagcgtttt ctgatcgggc aaccccacgg cctgttgcag cgtattttcc
       61 ttactcatgt aatagatagc cggcgtgaca tttgccccca gatcgtccat cagtttctca
      121 ttgtcactta acactttcat ttgctctgta cttacgtttg caggcacgtt tagcttaagc
      181 ttgccaccag aggcttcata ttgttgccag gtttttgcgg gatctttgga ggcaagaatt
      241 gccgctgctg tcgccgggct ttctggcttg ataaccccaa ccaacaatgt tcttaattgc
      301 actttgccag aatctaccca cgggcgcgcc tgctgccaga actgtttaca atatgggcag
      361 aacggatcgg cgaagacgta gacaatgacc ggcgcatctt ttttaccgtc gaggatccag
      421 tgggattgtt ccatccgttg ccacatttcg cgtccggctg gtgcgtaaat ttctttttcg
      481 ataagtgtgt tactcaggtt ttcacctttc tcgttgtaca tgtaaccaga gatagcgtgc
      541 ttaccatcgg gagtcaggta ggtagatggt gacgcccata tcctgatact ttccgagata
      601 acctttcatt cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat
      661 cgcttttact ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa
      721 aagtatcttt tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc
      781 ataacctatc actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg
      841 gtgttgtaat ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc
      901 gctgaagata ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac
      961 tttttatcgc cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa
     1021 aacttatcga tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac
     1081 atctcatcga aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt
     1141 ttaaaaacca ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag
     1201 gccgctggag cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac
     1261 tgggtgacgt tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag
     1321 tatctaccga tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta
     1381 aaatcaaata tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca
     1441 tgcgtgaaga tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca
     1501 tccaggcaat cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta
     1561 aaatcaaagc agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga
     1621 aagaaggtga agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct
     1681 tcgtctttcg cgctaccaac tccggcgcga aatacttcgg aaattcacct aattcttcgg
     1741 gtgctgcggc acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt
     1801 agaggccgct tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat
     1861 gaaaactcaa ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac
     1921 gctggatgac agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact
     1981 gtcagacaaa gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct
     2041 gatcaccaac ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga
     2101 gttcacctcg ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca
     2161 gtctctgctg gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc
     2221 gctctcttgc cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa
     2281 cccgcgcatc aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg
     2341 caacgtgatg ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat
     2401 gacgttgact gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga
     2461 gctgaacaag cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc
     2521 ggcagcaatt tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg
     2581 tggtcagatc ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg
     2641 gcagaagctg gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga
     2701 cagccagagc gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga
     2761 aacagcttct ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg
     2821 gcgcaacatg aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc
     2881 gcactgcgac ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc
     2941 cggcgtggaa gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt
     3001 tgcgccagaa atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt
     3061 cgacattatt ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg
     3121 tctggaatat cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt
     3181 cgtgcagatt ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg
     3241 catgggtgag attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc
     3301 gggtgactgt acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa
     3361 agcctctttg agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag
     3421 attcacctgc aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa
     3481 aagccctgcc gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga
     3541 ccagtacagg cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg
     3601 tcgaaatcga tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca
     3661 actcattgac ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat
     3721 cgatggtgaa gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct
     3781 cttcaaaacg acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta
     3841 gtacgtgaag tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag
     3901 ctttgtcgct caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa
     3961 ccctttctcc ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga
     4021 tatttgtctg gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac
     4081 accaggaatt ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt
     4141 aaagactgac tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg
     4201 gcccacatca cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata
     4261 ttattctgcg tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca
     4321 aaatacccca ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg
     4381 aaaccggaaa ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca
     4441 ttgcttgtgg cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca
     4501 atgcgggtaa aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg
     4561 gttatagtca cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta
     4621 aagggaatgt ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc
     4681 tttctgatat tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt
     4741 caagcgttgc agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt
     4801 tgctcggtgc ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg
     4861 ctgaccgcta cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa
     4921 ttattgaaca aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg
     4981 aagcgaaagg cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg
     5041 gtaaagcgtt gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg
     5101 gcgtctacgc tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga
     5161 cgtttaaaat gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg
     5221 agaaaggatt actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg
     5281 cccggggata tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac
     5341 ccggtgcaca aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc
     5401 cggggggaac aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata
     5461 atcgccgcca taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc
     5521 acaaacgaaa cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct
     5581 gattttgcgt aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca
     5641 gatgggatta aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt
     5701 caaggtgggt tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc
     5761 ccagcagtgc ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt
     5821 ttgccggata caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa
     5881 cccggctgtt cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc
     5941 gatccaactc tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca
     6001 gcagaatatc gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt
     6061 ttactccatg tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc
     6121 accgtcaggc ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa
     6181 gtgatttgcg tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc
     6241 gtcacggttt atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact
     6301 ttatcaatca caaaggtttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt
     6361 gcagaaagcg gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac
     6421 gcagggttac cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg
     6481 gcaaaggcgg cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat
     6541 tttccggctt cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg
     6601 tccggatcaa aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa
     6661 cgtccgccag ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg
     6721 gtttccggcg atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga
     6781 gtagcgcaac cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta
     6841 ggcaatcctg gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt
     6901 tgtaggcgac attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg
     6961 gtttgccagg agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac
     7021 gctacggcgt tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc
     7081 cagaatgctt tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc
     7141 ataaaaaagc ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc
     7201 gaacgtatta gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt
     7261 aaatcacccc gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc
     7321 cgtaaataat caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag
     7381 acagcaccag caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg
     7441 ccagaataac cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg
     7501 caaagtagaa caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt
     7561 gcgtactcat ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca
     7621 tcacaaccag agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat
     7681 ctttccaagg cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag
     7741 tagcattaat gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa
     7801 tcagtgtcca ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg
     7861 ttgccacttc ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacggcg
     7921 caatgataag caagataacc ccaaccggca ggaagcagag gaaccactgc aaccagctaa
     7981 tctggatacc ggcaattttg ctgacgaact ccagacccag cacgtttggt gccgcaccgg
     8041 tgacaaacat ggacgaactc agactggtac taatgaccat catccacatc aaatagccgc
     8101 caatacgacg cgcggacgga tcgttgggga atgatttaaa cagcggcggc aggtttttaa
     8161 tgaccggaaa aaccgtaccc ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca
     8221 gaatgtcgat aatgacaatc gcataaccca acgtcagcgt gcgtttgccc atgaatttca
     8281 ccaggaaaag ggcaatgcga cgacctaacc cggaaacttc ataccctaat gcaaaaataa
     8341 atgcgccaaa taccagccat accgtggtgc tggaaaaacc agccagaccc catttcagcg
     8401 cctgtttttg cgcattaaac gctgggtcag ctaattcttt ggcatcaaag agcaggtaat
     8461 tactgccaat aacgcaaata gtaaccgcaa taaaactgat cgctgttgcc ggaattggct
     8521 cgaggatcat gccgacaatc attgccacaa acacagcgaa gtaatgccat gcctgtggtg
     8581 gcataccgtc ggggacaggg ataagaaaca tgacacccat caccaccagt ggggccaata
     8641 gtttccatat attatctttt gctaaagaca tacgggttct ccgaaaatta atatttccaa
     8701 atttatcaag tgcttaaata attaaatctg tgctaaaaac caggtaagga tcagcaggtc
     8761 ggcactgccg cctggactga gatttcgttc gatacactcc ctgtcgaact gccggagata
     8821 atcgagatcg gcgggggttc gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg
     8881 tagccagcgc aggcccccct cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc
     8941 catcagtagg agcaaggtat cgagcaatgc cagttcagga tctaacccct gatccagcag
     9001 agtgaggtaa tgcggcaagg cgtgattgat caccagtgga taacccgctt cggcttcacc
     9061 gcgtgcgccg gtaaggccaa gctgctggta caaccgttga cctgccgtca gttgtgaatt
     9121 attggtacgc agttctcgat cggtcaggcc acggcagaaa cttgccgccg tagaacaaac
     9181 ggttgttggc gttaccggtt ggttgagttg aagcaaacgg ccaattgccg cacatagtag
     9241 ccctaaagaa aaaatgctgc ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc
     9301 accttcgcaa gccataccaa ttgggcgtaa tccgtggagt accgcttctg gtgctatttc
     9361 cgcactacag gcaccaaatt caatgaaacg gggtagccag ccctgaatcg ccagcgcgct
     9421 gcggtggaaa tcttccagcg ccatatcttt atgtgcaccg cagttaatcc gatccacgag
     9481 gcctggtttc ggtgacagat tgacttcagt cagcatggcg cgccagccca gcagggcgta
     9541 ctcatcgatc aatgacgtcg caagctttgt ggttttagtt gacgttgcag gcatcgacat
     9601 cgttcagcag tgcctccatg cggttgagta aatcggtcag ttgatgggtt tttccacgcg
     9661 cgcagacggc tgcgctttgc tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc
     9721 ggcgggagag aatttcgcct tcgggcgtca ggacatcgat atcccataac cgcccgagag
     9781 gatgactatg ttcaagctca atggtggcga gcttgaggtc gcgagccggg gcggcaatgc
     9841 tcaacatgcc ctccggcccg ctggcggaaa ccagtgcagc ctgctcctga atttgccagc
     9901 cctgttttgc ggctaaggca cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga
     9961 cctcgctgtc tttaatcggc ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat
    10021 ggcgcttgag ccagacgtgt tgccgtgctt gcctttcatc ccggctgacg agcagctcgg
    10081 gaattgacac cgcatggtgg ctggcgagtt caggaagcag gtgcatggct tattccttca
    10141 cctgatgcac aacatcgatc accgagccat cgcggtaacg cacaacggca acgacgcggt
    10201 cggtgaattc aatcggctgt ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact
    10261 caatggaaac cactttaatg cccgcttcct gcagacgttc tgccagttcc ggacgtgccg
    10321 ggttaactgc gataccgtgg tctgtgacca gaatatcgac actggagcct ggggtgatgc
    10381 aggtcagcac gttatccacc agagtcggaa tacgaccgcg taccagcggc gcgacgatga
    10441 tggaaagcgc agaggcaatc gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc
    10501 cgtcagagcc ggtcagcacg ttaacgttga actgggtgtc aatttccagc gcgctcagta
    10561 ccaccacgtc gagacgatca accgatgcgc ctttcgaacc ccagttagcg tactggttgg
    10621 cgctgatttc gatgtgattg gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc
    10681 tctgcacatc cagcagtttg cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg
    10741 taataccgcc gagggcgaag tcggcgcgaa tatcgcggct acgcattttg tcttccagga
    10801 aacgggttac cgccagcgat gcgccgccgg tgccggtttg catggagaaa ccttctttga
    10861 agtagccaga gttgacaatc acatccgcag cgctacgggc aataagcagt tcgcgcgggt
    10921 tagtggtcat acgggtcgcg ccagcgccga tttttgcagc atcgccaacg cggtcaactt
    10981 tgacgatcaa atcaacctga tcttgctcaa tgcttgccgg attatgcgga taaggcagca
    11041 gttcttcggt aagcatcaca acctgttttg cgttgtcggc atcaaccatt gcatagccga
    11101 gggagccgca gcaggcttta ccggtgtaac cgttggcatt accgaattca tcacaggacg
    11161 ggacgccgag gaaagccacg tcgatattca gttcgccgct ctgtaccaga tgcacacgtc
    11221 cgccgtgaga gtggatttgc accggttccg caagcagacc acgggaaatt tcttctgcca
    11281 gtggaccgcg caggccggag gtataaatgc gggtaaccac gccctggcga atgtgttcta
    11341 ccagtggcgc atggcaatca ctcagggagc tggacgccag ggtcaggttt ttaaagccca
    11401 tcttagcgat gacgtccatc accatattga cggtcaggtc accgccacgg aaagcgtgat
    11461 ggaaggaaac cgtcatgccg tcctgtaaac cagagcgacg aatcgcttct tcctggttgg
    11521 cgcacagttt gcgatcgcgc gctttttcag cctggtaggt ttgctttggt gagttctgga
    11581 aagcggcaag atcgcattca gcgcgacgat tccaggccgc tacccgttct tgtcgttgag
    11641 attgttcaat tttctgcgtc attttgattg ccttattctt cgcggatgcc ggaaagttct
    11701 gcacgggaga gcaccagacg ggcacgatcg ataaccggac cgtccaccat cttgccgttc
    11761 agggaaacca cgccgaggcc ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg
    11821 gcgtgatcca cttctttctg ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc
    11881 gggttgatca gcgatttgcc gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg
    11941 aatccggctt cgttgttagc gtcggaatag acggtatcga acgcctgaat acccgcagag
    12001 cgcgcggcct gcaaaatgga acagcgtgcg aacagcagtt cagttccttc cggggagcgt
    12061 tctgtacgca ggttgcgcac atagtcttct gcaccgaggg cgataccgat caaacgctcg
    12121 gaagcgtgag cgatttccac tgcgcgggta atccccagcg gagattcaat cgccgccagc
    12181 aggccggtgc tgccgggttc acgaccacag gctttttcga tacgcaggat ctctttttcg
    12241 atatccagaa catcctgagc ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg
    12301 cgaacgacgg cttccaggtc gttgacaccc cattcggaat ccagcgcgtt gacacgcaca
    12361 atggtttcaa tatcgcgata cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg
    12421 gtgtcttttt cacgcaatgc tacggagtct tcgaggtcaa acatcagggc atcagccggg
    12481 tagatgaagg agttgctgac catcgcggca ttggcaccag gcacaaacaa catgctgcgg
    12541 cgggtgcgag ttttacgttg ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca
    12601 gagccgggat accgctggcg cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt
    12661 ccagtgcgcc tttgtcatcg acattcagct gtacgccgcg cacgttgtag cgggcgagaa
    12721 cgtccagaat ggtggtgcga attgcatcgc caaactgttt ctcaacgctg ctattgattt
    12781 gcaggtcgat atcctgcgta tcgagtgggg cgatgcgtat catcacatcc ccagactcaa
    12841 gggtgcctgc aacggcgggc tggtttattt tcatttttca cctgtttctc atgcgggggt
    12901 cttttgacga gctgccgcgt cctggcggga gtgctcaagc aggttctgca aataatgcag
    12961 cgtgactgca gggaccagcg gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg
    13021 tacccgggaa gcggatatcg gcatctcctg gtaacgcagc cgctcaattt caaccagttc
    13081 gatgggcggt gcggagatag tcggcgtttc cagccagtag cgcatatcct ggttgtactg
    13141 ggcggtaacg cgacaaaagg gttcagtacc gacaaagcgg tgagttacac ccagcgcggg
    13201 agcgaggtac tgacggaaaa tcttcagatc aatttcggtg taacaatggt taatgacgct
    13261 ctgttcttta atgaagtagc aagggaacgt agcgcgggag atgatgtatt cggagccgcg
    13321 atgcacagtc aggcgtggaa tatcggcggt gccttttaac accagatcca accggtcttc
    13381 ataggggaag cgtgaagaat cttctttgac taaaaacaga tgcaaccagt cgcactgtgc
    13441 cgcagcttgt tgaatcagat aacggtgacc attcgtgaag ggattggcgt tcatcacaat
    13501 gcagccaatc ttgttccctg gatgacgaaa ttttttcagc gattcggcat agcgtttcag
    13561 ccgcgtggcg ctgttttcca tcagcaccat cacgccgggt acgctggtca gcgtggaaaa
    13621 accgcactgg cggaacagcg cctcgtattc ggttttggta taaataaaca gatgcgtgct
    13681 gtgccgctca taggcgaggt ttatcaattc agtggctaat gtcagcgcca gtccttcacc
    13741 gcgaacggat tcactgatag caacgcattt aataatattt ccggcaattc caccgcacgt
    13801 gataagcttt tcatcgcggg ttacggtaat aaatacttcg actgtggtgt caacg
//
LOCUS       DAZPLM010000105        13738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPLM010000105 DAZPLM010000000
VERSION     DAZPLM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.105"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /gene="yojO"
                     /locus_tag="ACHVC7_002588"
     CDS             complement(<1..119)
                     /gene="yojO"
                     /locus_tag="ACHVC7_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR2116608.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAI"
     gene            228..722
                     /gene="napF"
                     /locus_tag="ACHVC7_002589"
     CDS             228..722
                     /gene="napF"
                     /locus_tag="ACHVC7_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR2116609.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            712..975
                     /gene="napD"
                     /locus_tag="ACHVC7_002590"
     CDS             712..975
                     /gene="napD"
                     /locus_tag="ACHVC7_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR2116610.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            972..3458
                     /gene="napA"
                     /locus_tag="ACHVC7_002591"
     CDS             972..3458
                     /gene="napA"
                     /locus_tag="ACHVC7_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR2116611.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3465..4160
                     /gene="napG"
                     /locus_tag="ACHVC7_002592"
     CDS             3465..4160
                     /gene="napG"
                     /locus_tag="ACHVC7_002592"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR2116612.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4147..5010
                     /gene="napH"
                     /locus_tag="ACHVC7_002593"
     CDS             4147..5010
                     /gene="napH"
                     /locus_tag="ACHVC7_002593"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR2116613.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5007..5456
                     /gene="napB"
                     /locus_tag="ACHVC7_002594"
     CDS             5007..5456
                     /gene="napB"
                     /locus_tag="ACHVC7_002594"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR2116614.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5466..6068
                     /gene="napC"
                     /locus_tag="ACHVC7_002595"
     CDS             5466..6068
                     /gene="napC"
                     /locus_tag="ACHVC7_002595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR2116615.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6081..6704
                     /gene="ccmA"
                     /locus_tag="ACHVC7_002596"
     CDS             6081..6704
                     /gene="ccmA"
                     /locus_tag="ACHVC7_002596"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR2116616.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6701..7363
                     /gene="ccmB"
                     /locus_tag="ACHVC7_002597"
     CDS             6701..7363
                     /gene="ccmB"
                     /locus_tag="ACHVC7_002597"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR2116617.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7405..8142
                     /gene="ccmC"
                     /locus_tag="ACHVC7_002598"
     CDS             7405..8142
                     /gene="ccmC"
                     /locus_tag="ACHVC7_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR2116618.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8139..8348
                     /gene="ccmD"
                     /locus_tag="ACHVC7_002599"
     CDS             8139..8348
                     /gene="ccmD"
                     /locus_tag="ACHVC7_002599"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR2116619.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8345..8824
                     /gene="ccmE"
                     /locus_tag="ACHVC7_002600"
     CDS             8345..8824
                     /gene="ccmE"
                     /locus_tag="ACHVC7_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR2116620.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8821..10764
                     /gene="ccmF"
                     /locus_tag="ACHVC7_002601"
     CDS             8821..10764
                     /gene="ccmF"
                     /locus_tag="ACHVC7_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR2116621.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10761..11318
                     /gene="dsbE"
                     /locus_tag="ACHVC7_002602"
     CDS             10761..11318
                     /gene="dsbE"
                     /locus_tag="ACHVC7_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR2116622.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11315..12367
                     /gene="ccmH"
                     /locus_tag="ACHVC7_002603"
     CDS             11315..12367
                     /gene="ccmH"
                     /locus_tag="ACHVC7_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR2116623.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12402..13049)
                     /gene="narP"
                     /locus_tag="ACHVC7_002604"
     CDS             complement(12402..13049)
                     /gene="narP"
                     /locus_tag="ACHVC7_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR2116624.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13369..>13738
                     /locus_tag="ACHVC7_002605"
     CDS             13369..>13738
                     /locus_tag="ACHVC7_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR2116625.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2935 a         3461 c         4035 g         3307 t
ORIGIN      
        1 ttaatcgcta tataaatatg tttataacca tttgaaatgt gagcaaaagc ccgtttttgc
       61 cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg ggtatgcatc
      121 tttgacacat cctttaatat cttagcggct ataaaaatgg cttattaatt atgcggttta
      181 tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg aagattgatg
      241 catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt atccgtccgc
      301 cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac gcctgtatta
      361 atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc gttaatttca
      421 aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc ttgccccgaa tcgctatttt
      481 ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg tgcctggcgt
      541 atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca attatctttc
      601 gcccgacgct ctccgggatc taccagccgc aacttaatag ccaactttgc aacggatgtg
      661 gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc catgcacact
      721 aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc agacatcagc
      781 acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc gagcggtcag
      841 ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat tgagtcagta
      901 cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga agagcaaggt
      961 gaggaaacac catgaaactc agtcgtcgta gctttatgaa agctaacgcc gttgcggccg
     1021 ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg cgccgttgtt ggtcagcagg
     1081 aagccattaa atgggataaa gcgccgtgcc gtttctgcgg tactggttgc ggcgttctgg
     1141 tcggaacgca gcaggggcgt gtggtggcct gtcagggcga cccggacgca ccggttaacc
     1201 gtggcctgaa ctgcattaag ggctatttcc tgcccaagat catgtacggt aaagaccgtt
     1261 tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga caaagaaggc gaatttaccc
     1321 caatcacctg ggatcaggcc ttcgatgtga tggaagagaa attcaaaacc gccctgaaag
     1381 aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca gtggactatc tgggaaggtt
     1441 atgccgcgtc caagctgttt aaagcgggct tccgttcgaa caacatcgac ccgaacgcgc
     1501 gtcactgtat ggcgtcggca gtagttggct ttatgcgtac ctttggtatg gatgagccga
     1561 tgggctgcta tgacgacatc gagcaggctg acgcgtttgt gctgtggggc gcaaacatgg
     1621 cggagatgca cccgatcctc tggtcgcgca tcactaaccg tcgtctctct aatcagaacg
     1681 tcaccgtggc ggtgctttct acctaccagc atcgtagctt cgagctggcg gataacggca
     1741 tcatctttac gccacagtct gacctggtga tcctgaacta catcgccaac tatatcattc
     1801 aaaacaatgc gataaatcag gacttcttca gcaaacacgt taacctgcgc aaaggggcga
     1861 cggacatcgg ctacggttta cgtccgaccc atccgctgga aaaagcagcg aagaatccag
     1921 gttctgacgc ctccgaaccg atgagctttg aagattacaa agccttcgtt gccgagtata
     1981 cgctggaaaa aaccgctgaa atgaccggcg tgccgaaaga ccagttagaa caactggcgc
     2041 agctgtatgc cgatccgaac aagaaagtca tctcctactg gacgatgggc ttcaaccagc
     2101 atactcgtgg cgtgtgggct aacaacctgg tctacaacct gcacctgctg accggcaaaa
     2161 tttcccagcc gggttgcggt ccgttctccc tgaccgggca accttctgcg tgtggtactg
     2221 cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga catggtggtg accaacgaga
     2281 aacaccgtga tatctgcgag aagaagtgga atatcccgag cggcaccatt ccggcgaaaa
     2341 tcggtctgca cgcggtggca caagaccgtg cgctgaaaga cggcaaactg aatgtttact
     2401 ggaccatgtg taccaacaac atgcaggccg ggccgaacat caatgaagag cgtatgccgg
     2461 gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc gtatccgaca gtcagtgcgc
     2521 tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga gaaagagggt gcttacggta
     2581 acgccgaacg ccgtactcag ttctggcgtc agcaggtaca ggcaccgggc gaagcgaaat
     2641 cggatctctg gcagttagtc cagttctccc gccgcttcaa aactgaagaa gtgtggccgg
     2701 aagagctgct agcgaagaaa ccggaactgc gtggcaaaac gctgtacgaa gtgctttatg
     2761 ccacaccgga agtgagcaaa ttcccggtat ccgaactggc ggaagatcag ctgaacgatg
     2821 aatcccgcga gctgggcttc tatctgcaaa aagggctgtt cgaagagtac gcatggtttg
     2881 gtcgcggtca cggtcacgat ctcgcgccgt tcgatgacta ccacaaagcg cgcggtctgc
     2941 gctggccggt ggtgaacggt aaagaaacgc agtggcgtta cagcgaaggt aacgacccgt
     3001 acgtgaaagc gggcgaaggc tataagttct acggtaaacc ggatggtaaa gcggtgatct
     3061 tcgcgctgcc gttcgaaccg gcggcggaag caccggatga agagtacgac ctgtggctct
     3121 ctaccggacg cgttctggag cactggcaca ctggcagtat gactcgccgt gtgccggaac
     3181 tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc gctggatgcg aaagcgcgcg
     3241 atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg tggcgaagtg atctcgattg
     3301 ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt gtacatgccg ttcttcgacg
     3361 ccgcacagct ggttaacaaa ctaacgctgg acgcgaccga tccgctctcg aaagagacgg
     3421 acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc cattatgtcc cggtcagcga
     3481 aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt tcgcacagca ggcgggctgg
     3541 ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc acgcgcatct ggcgtgcggt
     3601 tgcgcccgcc cggagccata aacgagaacg cctttgccag tgcctgtgtg cgttgcggtc
     3661 agtgtgttca ggcttgccct tacgacacct taaaactggc gacgctggct tctggcctgt
     3721 cggcgggcac gccatatttt gtcgcacggg atattccttg cgaaatgtgt gaggacattc
     3781 cgtgcgccaa agtgtgcccg agtggtgcgc tggatcgtga gatcgaatcg atcgacgacg
     3841 cgcggatggg gctggcggtg ctggtggacc aggaaaactg tctcaacttt caggggctgc
     3901 gctgcgatgt ttgttatcgc gaatgcccga aaattgatga ggccatcacc ctggagctgg
     3961 agcgcaacac gcgtaccggt aagcacgccc gctttctgcc gacggttcac agcgacgcct
     4021 gtaccggctg cggtaagtgc gaaaaagtgt gcgtgctgga acaaccggca atcaaggtgt
     4081 taccgctgtc actggcgaaa ggggagttag gtcaccatta ccgcttcggc tggctggagg
     4141 ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg cgctggtgaa aaaaggctgg
     4201 tggcgcagtc accgttggct ggtgttacgt cgcctttgtc agttcttcgt gctggggatg
     4261 tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg gcaactacag ctgtagcctg
     4321 ttattcgaca ccgttccgct gaccgatccg ctgatgacgc tgcaaagtct tgccagtggt
     4381 catctgcctg ccacggtggc gttgaccggt gcggtgataa tcactgtgct ctacgccctg
     4441 gcaggaaagc gattattttg cagctgggtc tgcccgctga acccgattac cgacttagcg
     4501 aactggttac gcaggcgctt tgacctcaat cagtctgcga cgatccctcg ccacattcgc
     4561 tacgtgctgt tggtggtcat tctgctggga tcagccttaa ccggcacgct catctgggaa
     4621 tggatcaacc ccgtttccct gatggggcgt agcctggtaa tgggcttcgg cagcggcgcg
     4681 ctgcttattc tcgcgctgtt tttatttgat ctactggtcg ttgaacacgg ctggtgcggg
     4741 cacatttgtc cggttggcgc gctgtatggc gtgctgggta gcaaaggtgt gattaccgtt
     4801 gcggccagcg atcggcagaa atgtaaccgc tgtatggatt gttttcatgt ttgcccggaa
     4861 ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc cggtgcaggt caccagccgc
     4921 gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg aggatgtatt tacaataact
     4981 acacgatgga gttcgggagc gaaatcatga aaagccatga cctgaagaaa gcgctgtgtc
     5041 aatggacggc gatgctggcc ctggtggtaa gcggcgcggt ttgggcggct aatggcgtcg
     5101 attttagcca gtcgccggaa gtatccggga cgcaggaagg ggccattcgg atgccaaaag
     5161 agcaggatcg gatgccgctg aactatgtga atcagccgcc aatgatcccg catagcgttg
     5221 aaggttatca ggtaacgacc aataccaacc gctgcttgca gtgccacggt gtcgaaagct
     5281 atcgcaccac tggcgcgccg cgtatcagtc ctactcactt tatggacagc gacggcaaag
     5341 tgggcgcgga agtggctccg cgtcgttatt tctgtctgca atgtcacgta ccgcaggccg
     5401 ataccgcgcc aatcgtgggg aataccttta ccccatcaaa aggttacggg aaataagagg
     5461 ttattatggg aaattctgac cgtaagcctg gtctgattaa gcgcctgtgg aaatggtggc
     5521 gtacccccag ccgtctggcg ctgggaacgc tgctgttgat cggttttgtc ggcggcatcg
     5581 tcttctgggg cggctttaac accggaatgg aaaaagccaa taccgaagag ttctgcatta
     5641 gctgccacga aatgcgtaac acggtgtatc aggaatacat ggattccgtg cactacaaca
     5701 accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt tccgcacgag tttgtgccga
     5761 agatgatacg caagctcaaa gcaagtaaag agctgtatgg taaaattttt ggcgttattg
     5821 acacgccgca gaaatttgaa gctcatcgtc tgacaatggc acagaatgag tggcggcgca
     5881 tgaaggacaa taactcgcag gagtgccgta actgtcacaa cttcgagtat atggatacaa
     5941 ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc ggtgaaagat gggcaaacct
     6001 gtattgattg ccataaaggg atagcgcaca agctgcccga tatgcgtgaa gtcgagccag
     6061 gtttttaaca gggttattgc gtgggtatgc ttgaagccag agagttactt tgtgagcggg
     6121 atgaacgaac cttatttagt ggcttgtcat ttacgctgaa cgcaggagag tgggtacaaa
     6181 tcaccggtag caacggcgcg gggaagacaa cgcttctccg tttgctgacg gggttgtctc
     6241 gccctgacgc aggcgaggtt ctctggcaag ggcagccctt gcatcaggta cgcgacagct
     6301 accatcaaaa cctgttatgg ataggccatc agccggggat caaaacccgg ctgacggcgt
     6361 tagaaaatct gcacttttat catcgcgatg gcgataccgc acaatgtctg gaagccctgg
     6421 cgcaggccgg gcttgccgga ttcgacgata ttccggtaaa tcaactttct gccgggcaac
     6481 aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc cacgttatgg atcctcgacg
     6541 agccttttac cgcgattgac gttaacggcg tcgatcgtct gacccagcgt atggcgcagc
     6601 atacggagca gggggggatt gtgattctga ctacccacca gccgctcaac gttgctgaaa
     6661 gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc atgatgttct ggcgcatttt
     6721 ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa atcgccaacc cgctgtggtt
     6781 cttcctgatt gtaattaccc tttttccgct cagtatcggt ccggagccgc aactgctggc
     6841 gcgtattgca ccgggcatta tctgggttgc tgcgctgctt tcatccttgc tggcgctgga
     6901 acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa caattgatgt tgttgccgtt
     6961 acccttgccc gccgttgtgc tggcgaaggt gatggcgcac tggatggtaa ccggtctgcc
     7021 gttactcatc ctttcgccac tggtagcaat gctactggga atggatgttt atggctggca
     7081 agtgatggcg ctgacgctgc tgctgggaac gcctacgctt ggctttctcg gtgcaccggg
     7141 tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg ctcagcatac tggtgttacc
     7201 gctgactatc ccattactca tctttgccac cgccgcgatg gacgcggctt ccatgcattt
     7261 gcccgttgac gggtatctgg caattttagg cgcgttgctg gcaggcaccg cgacattaag
     7321 tccttttgcg acggcggcag cgttacgaat cagcattcaa taacgctgga ttactgattt
     7381 ttgttcgagt ctggtatcga aactatgtgg aaaacactgc atcaactggc gatcccacca
     7441 cggctgtatc aaatctgtgg ctggtttata ccgtggctgg caattgccag tgtggtcgtg
     7501 cttaccgtcg gctggatctg gggattcggc tttgctccgg ctgattatca gcagggaaat
     7561 agctaccgca ttatctacct gcatgtgcct gcggcgatct ggtcgatggg catttatgca
     7621 tcaatggcag tggcagcgtt tattggcctt gtctggcaga tgaaaatggc caacctggcg
     7681 gtggcggcga tggcccccat tggtgccgtg tttaccttta ttgccctggt taccggctct
     7741 gcatggggaa aaccgatgtg gggcacctgg tgggtatggg atgcacgtct gacttctgaa
     7801 ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt ggcacgcctt cgacgaccgc
     7861 cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg gcgtggtgaa tctgccgatt
     7921 attcattact ccgtggagtg gtggaacacc ctgcatcagg gatcaacgcg gatgcagcaa
     7981 agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga tttttggctt cctgctcctg
     8041 tctgccacgc tgacgctgat gcggatgcgt aatttgattt tgctgatgga aaaacgccgt
     8101 ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat gacccctgca tttgcttcct
     8161 ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt ctggctggcg gtggtgatga
     8221 ccgttattcc gctggtggtt ttggtcgtgc actcggtgat gcaacatcgc gcaattctgc
     8281 gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg tgctgcgcaa cagcaggagg
     8341 ctgcatgaat attcgccgta aaaaccgctt gtggattgcc tgtgccgtgt tggcagggct
     8401 ggcgctgact atcggtctgg tgctatatgc gctgcgctcg aatatcgatc tcttttatac
     8461 gccgggggaa attctctacg gcaagcgtga aactcagcaa atgccggaag tcggtcagcg
     8521 tctgcgcgtt ggcgggatgg tgatgctggg tagtgtgcag cgcgatccca attcgctgaa
     8581 agtgaccttc actatttacg atgctgaagg ctcagtggat gtctcttacg aaggcatttt
     8641 gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag ggcgagctgg aaaaaggcaa
     8701 tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa aactacacgc cgccagaagt
     8761 tgagaaagcg atggaagcta accaccgtcg cccggcgagt gtttataagg acccagcatc
     8821 atgatgccgg aaattggaaa cgggctgctg tgtctggcgt tagggattgc gctgctgctg
     8881 tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc gcatgatcgc gtcttcccgc
     8941 ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat ttctggtgct ggtcaatgct
     9001 ttcgtggtca acgactttac cgtcacctat gttgccagct actccaatac ccagcttccg
     9061 gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag gctcgctact gctgtgggtg
     9121 ctgctgatga gcggctggac ctttgcggtg gcgattttta gtcagcgtat tccgctggat
     9181 attgtggccc gcgtactggc gataatgggg atggtcagcg tcggcttttt gctgttcatt
     9241 ctctttacct ctaacccatt ctcacgcacg ctgccgaact tcccgattga agggcgcgat
     9301 cttaacccgc tgttacagga cccggggctg atcttccatc cgcctctgct ttatatgggg
     9361 tacgtgggtt tctcggtggc gtttgctttt gccattgctt ctttgctgag cgggcgtctg
     9421 gacagcactt atgcgcgttt tactcgcccg tggacgctgg cggcgtggat tttcctgacg
     9481 ctcggcattg tgctcggttc cgcatgggcc tattacgaac tcggctgggg gggctggtgg
     9541 ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc tggtggggac tgcgctgatg
     9601 cactcactgg cggtcactga acaacgcgcc agcttcaaag cgtggacatt actgctggca
     9661 atcagtgcct tctcgttgtg tctgttgggg actttcctcg tgcgttccgg cgtgctggta
     9721 tcggtacacg cgtttgcgtc tgatccggcg cgcggtatgt ttatcctcgc ctttatggtg
     9781 ctggtgattg gaggttcgct gctgctgttt gccgcgcgtg gacacaaagt tcgctcacgc
     9841 gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag cgaacaacgt tttgctggtc
     9901 gccgcgatgc tggtggtgtt gctggggacg ctgctgccgc tggtgcacaa gcaactggga
     9961 ctgggcagta tttcgattgg cgaaccgttc ttcaacacca tgtttacctg gctgatggtg
    10021 ccgtttgcgc tactgctggg tgtcggtcct ctggtgcgct gggggcgcga tcgcccacgt
    10081 aaaatccgca atttattgat tatcgccttc atctcaacgc tggtgctgtc tctgctgttg
    10141 ccgtggctgt ttgagagcaa agttgtggcg atgacggtgc tcggcctggc aatggcctgc
    10201 tggattgcgg tgctggcaat tgcggaagct gcgctgcgta tttcacgcgg cacgaaaacc
    10261 accttcagtt attgggggat ggtggctgct cacctggggc tggcagtgac aattgttggc
    10321 attgctttta gccagaacta tagcgttgag cgtgatgtgc gcatgaagtc cggcgatagc
    10381 gtcgatattc atgaatatcg cttcaccttc cgtgatgtca aagaggtgac tggcccgaac
    10441 tggcgtggcg gtgtggcgac tatcggcgta acgcgagagg gtaaaccgga aacagtgctg
    10501 tatgcggaaa aacgttatta caacactgcc gggtcgatga tgaccgaagc ggcgattgac
    10561 ggtggcatca cgcgtgacct gtacgccgcg ctcggtgaag agctggaaaa cggcgcgtgg
    10621 gctgtgcgtc tttactacaa accatttgtt cgctggattt gggcgggcgg gctgatgatg
    10681 gcgttgggcg gactgctgtg tctgtttgat cctcgctatc gtaagcgcgt gagtccgcaa
    10741 aaaactgcgc cggaggctgt atgaagcgca aagtattgtt aattccgttg attatcttcc
    10801 tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa tgccgaaggg gatgatccga
    10861 ccaatctgga atcggcgctc attggcaagc cagtgccgaa gtttcgcctc gaatcactgg
    10921 acaatccggg gcagttttat caggcggatg tgctgactca gggcaaacca gtgctgctta
    10981 acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca tcaatatctg aatcagcttt
    11041 ctgcgcaggg catccgcgta gtcggcatga actataaaga cgatcgccag aaggcaatca
    11101 gctggctgaa agagctgggc aatccttacg cgctaagcct gtttgatggc gatggcatgt
    11161 tagggctgga tctcggtgtc tatggcgcgc cagaaacgtt ccttattgac ggcaacggca
    11221 tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt ctgggaagaa gagatcaagc
    11281 cgctgtggga gaaatacagt aaggaggccg cacaatgagg tttttattgg gcatgctgat
    11341 gctgatgatc tccggctcag cgctggcgac catcgacgtg ttgcagttta aagatgaagc
    11401 acaggaacaa cagttccgtc agctcactga agaactgcgc tgcccgaaat gccagaacaa
    11461 cagcattgcc gattccaact cgatgattgc caccgacctg cgccagaaag tgtatgaact
    11521 gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat atggtggcgc gttacggcaa
    11581 cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg ctgctgtggg tgctgccagt
    11641 agtagctatt ggcattggcg gttgggtcat atacgcccgc tcgcggcgtc gggtacgcgt
    11701 ggtgccggaa gcgtttcctg aacaaagcgt gccggaaggt aagcgtgccg gatatgtcgc
    11761 ttatttgccg ggcattgtgg tggcgttaat tgtggctggc gtcagctact accagaccgg
    11821 aaattatcaa caggtgaaaa tctggcagca ggccacggca caggctccgg cgttgctgga
    11881 cagggcgctg gatccgaaag ctgatccgct caacgaagaa gagatgtcgc gccttgcgct
    11941 ggggatgcgt actcaactgc aaaaaaatcc gggagatata gacggctgga ttatgttggg
    12001 ccgcgttggc atggcgctgg gtaacgccag tatcgccacc gatgcatacg ctactgcgta
    12061 tcgcctcgat ccgaaaaaca gtgatgccgc actgggttat gctgaagcgt tgacacgttc
    12121 atctgatccc aacgacaacc gcctcggcgg cgaactgcta cgtcagctgg tgagaacgga
    12181 ccatagcaat atccgtgtgc taagcatgta tgcgtttaat gcctttgagc agcagcgatt
    12241 tggcgaagcc gttgccgcgt gggagatgat gttgaaactc ttacctgcca acgatactcg
    12301 ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa catttgtcgc cgcaggagag
    12361 taaataaaaa gaggcccgat ggtgatgcca tcgggctatt tttattgtgc cccgcgttgt
    12421 tgcaggaaca gaatggtggc cgccacgcgt gagcggacat tgagtttacg cagcagattt
    12481 cgaatatgaa cttttactgt ctgctcggaa atattcaaca cagaggcaat ctgtttattt
    12541 gacagcccct gtgccagctc gtgcagaaca tccagctcgc gctccgtcag cacgctgaag
    12601 ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt actgattgac gcgttcgcta
    12661 aagactttgc tgcctttcgc tccggcacga atcgcttcca gcaacacttc cgggtcgctg
    12721 tctttcagca gataaccgtc tgcgcctgcg tctatcagcg caaagacatc gctggaggca
    12781 tcggatacgg tcaggataat aatttgcgcg gtaacgccat ccctgcgcaa ggcattgagg
    12841 gtatccaggc cactcatacc tttcatattg agatccagca agatcacgtc gatatccagt
    12901 ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt cggcgaccac ttcaaagcca
    12961 ggatcaagct ccagtaactg acgaacaccg cgtcgcataa gtggatgatc atccacaatc
    13021 atcacctgaa aaggcgttgc ttcaggcata gtagtctcct gaggttttat tagaatcatt
    13081 atgtttgagc gtagcgttgg taacgctacc aggcgcgtga atgttacccc attttatgta
    13141 tggacatgta gtgaaaacta caaccttgcc gttcgctttg gcatcatttt cattataaag
    13201 acggaatttt gcatcagctt ctcatcaaga aaaacaacta aaaaatcaat cacaatactt
    13261 gtgcatttct ttaaacagtt tttaaaaact gactccgggt atggagctat gggtattttc
    13321 tgtacccaat gcttttaaca gcaattaatt tcataggatg aaagctcaat gcatcaatct
    13381 ggttctgttt ctctttgtcg ttccgcaata tctgttctgg tggctacagc gttatattca
    13441 cccatagcat tggcatcaac agttgagtat ggtgagacag ttgatggtgt tggcctggaa
    13501 aaagatatcc agctggttta tgggaccgcc aataatacga aaatcaatcc tggcggagaa
    13561 cagcatataa aagaatttgg tgtaagtagt aatactgaaa ttaacggcgg gtatcagtac
    13621 attgaaatga atggcactgc agaatactca gtattaaatg atggttatca aattgttcaa
    13681 atgggtggcg cggtaaacca gactacgctc aataatggag tgctacaggt ttatggcg
//
LOCUS       DAZPLM010000106        13524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPLM010000106 DAZPLM010000000
VERSION     DAZPLM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.106"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHVC7_002606"
     CDS             complement(<1..81)
                     /locus_tag="ACHVC7_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR2116626.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHVC7_002607"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHVC7_002607"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR2116627.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHVC7_002608"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHVC7_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR2116628.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHVC7_002609"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHVC7_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR2116629.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHVC7_002610"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHVC7_002610"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR2116630.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHVC7_002611"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHVC7_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR2116631.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHVC7_002612"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHVC7_002612"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR2116632.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHVC7_002613"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHVC7_002613"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR2116633.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHVC7_002614"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHVC7_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHVC7_002615"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHVC7_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR2116634.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHVC7_002616"
     CDS             complement(12387..12626)
                     /locus_tag="ACHVC7_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR2116635.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHVC7_002617"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHVC7_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR2116636.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHVC7_002618"
     CDS             13003..13290
                     /locus_tag="ACHVC7_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR2116637.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3523 a         3322 c         3479 g         3200 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtt
//
LOCUS       DAZPLM010000107        13449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPLM010000107 DAZPLM010000000
VERSION     DAZPLM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.107"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1710
                     /gene="adeD"
                     /locus_tag="ACHVC7_002619"
     CDS             <1..1710
                     /gene="adeD"
                     /locus_tag="ACHVC7_002619"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR2116638.1"
                     /translation="LLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVI
                     DVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEKFAFTTLE
                     VTE"
     gene            complement(1755..3146)
                     /gene="uhpT"
                     /locus_tag="ACHVC7_002620"
     CDS             complement(1755..3146)
                     /gene="uhpT"
                     /locus_tag="ACHVC7_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR2116639.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3284..4603)
                     /gene="uhpC"
                     /locus_tag="ACHVC7_002621"
     CDS             complement(3284..4603)
                     /gene="uhpC"
                     /locus_tag="ACHVC7_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR2116640.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4613..6115)
                     /gene="uhpB"
                     /locus_tag="ACHVC7_002622"
     CDS             complement(4613..6115)
                     /gene="uhpB"
                     /locus_tag="ACHVC7_002622"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR2116641.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6115..6705)
                     /gene="uhpA"
                     /locus_tag="ACHVC7_002623"
     CDS             complement(6115..6705)
                     /gene="uhpA"
                     /locus_tag="ACHVC7_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR2116642.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6780..7070)
                     /gene="ilvN"
                     /locus_tag="ACHVC7_002624"
     CDS             complement(6780..7070)
                     /gene="ilvN"
                     /locus_tag="ACHVC7_002624"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR2116643.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7074..8762)
                     /gene="ilvB"
                     /locus_tag="ACHVC7_002625"
     CDS             complement(7074..8762)
                     /gene="ilvB"
                     /locus_tag="ACHVC7_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR2116644.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8868..8966)
                     /gene="ivbL"
                     /locus_tag="ACHVC7_002626"
     CDS             complement(8868..8966)
                     /gene="ivbL"
                     /locus_tag="ACHVC7_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR2116645.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9531..9620
                     /gene="tisB"
                     /locus_tag="ACHVC7_002627"
     CDS             9531..9620
                     /gene="tisB"
                     /locus_tag="ACHVC7_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR2116646.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9744..9818)
                     /gene="ysdE"
                     /locus_tag="ACHVC7_002628"
     CDS             complement(9744..9818)
                     /gene="ysdE"
                     /locus_tag="ACHVC7_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR2116647.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9900..11084
                     /gene="emrD"
                     /locus_tag="ACHVC7_002629"
     CDS             9900..11084
                     /gene="emrD"
                     /locus_tag="ACHVC7_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR2116648.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11092..11589)
                     /locus_tag="ACHVC7_002630"
     CDS             complement(11092..11589)
                     /locus_tag="ACHVC7_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR2116649.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11586..11948)
                     /locus_tag="ACHVC7_002631"
     CDS             complement(11586..11948)
                     /locus_tag="ACHVC7_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR2116650.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11938..12285)
                     /locus_tag="ACHVC7_002632"
     CDS             complement(11938..12285)
                     /locus_tag="ACHVC7_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR2116651.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12394..12843
                     /gene="yidI"
                     /locus_tag="ACHVC7_002633"
     CDS             12394..12843
                     /gene="yidI"
                     /locus_tag="ACHVC7_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR2116652.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12890..13048)
                     /locus_tag="ACHVC7_002634"
                     /pseudo
     CDS             complement(12890..13048)
                     /locus_tag="ACHVC7_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13092..>13449)
                     /locus_tag="ACHVC7_002635"
     CDS             complement(13092..>13449)
                     /locus_tag="ACHVC7_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2116653.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3158 a         3762 c         3525 g         3004 t
ORIGIN      
        1 ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa tgtctctatt
       61 ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt
      121 gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga tgctcgcggc
      181 gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg
      241 ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct
      301 catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg ctgtgccgaa
      361 caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc
      421 gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag
      481 gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg
      541 ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt
      601 ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga aagttatcag
      661 ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc
      721 gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc
      781 atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc
      841 ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc
      901 agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggcaag
      961 caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg
     1021 aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa
     1081 tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc
     1141 ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt
     1201 acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgtgcttt
     1261 attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt gcttggcggc
     1321 tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca taatattgtg
     1381 gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc
     1441 ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt gcccattgcc
     1501 ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc cttgaaagcc
     1561 gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc gtttctttct
     1621 ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt cgagaagttt
     1681 gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg ccgggcaaaa
     1741 gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg
     1801 cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatca
     1861 agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc
     1921 attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata
     1981 ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac
     2041 aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg
     2101 ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc
     2161 accaggccac ggcgaccgtt cgctaggtca gagagccagc cccacagcag cgtaccgacc
     2221 agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt
     2281 tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa
     2341 atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc
     2401 tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg
     2461 aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg
     2521 ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatgacatgg
     2581 ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc accgccaagg
     2641 ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg
     2701 gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag
     2761 aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc
     2821 atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt tttgccgtcg
     2881 gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc caggccgatc
     2941 atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg cgcgatgtta
     3001 aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc
     3061 atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg
     3121 cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca
     3181 tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa
     3241 atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcgcgcggtg
     3301 tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa
     3361 ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc caaccagcaa
     3421 gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt
     3481 tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag acaaaaaaac
     3541 caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacaggg
     3601 agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct cggttgccgt
     3661 taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca cccagttcaa
     3721 acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca
     3781 aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa
     3841 gcagccagat atacggattc agcaacacat atttggtgag gatctcttta cgcgtcagcc
     3901 ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca ccgaccgccg
     3961 gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt cccacgacga
     4021 tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt gcagccgctg
     4081 ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc
     4141 aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac
     4201 cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat agcgacgtcg
     4261 agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc ataaaataac
     4321 gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca taggtaatgt
     4381 aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt
     4441 ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa cccagccaga
     4501 tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca tatttatcag
     4561 ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa ccttagacat
     4621 agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc
     4681 cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac
     4741 ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca tcctgctgcc
     4801 agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc agcccttcct
     4861 ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca tcgattcgcc
     4921 attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc agtgagcgga
     4981 tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc aacaaacggc
     5041 gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc tgcttcacgc
     5101 tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata gcagtgatgg
     5161 tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc tcttcggttt
     5221 ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc gactggttaa
     5281 gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt tgcaccagca
     5341 gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg
     5401 cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc agcgcgataa
     5461 tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga
     5521 gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg cgccagttga
     5581 ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc
     5641 agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat
     5701 tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta
     5761 acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca
     5821 gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa tgggttaaac
     5881 cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat acgggccagt
     5941 atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg
     6001 ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac catgcggcag
     6061 agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca
     6121 gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt tttccatcag
     6181 attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg caatctcttt
     6241 caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg ttagcgggtc
     6301 ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc cacccgtggc
     6361 aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa gaaagccgcg
     6421 tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg agagcataat
     6481 cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat cgggcatgga
     6541 gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc
     6601 cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc
     6661 ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg
     6721 ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt
     6781 tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt acgctgcact
     6841 ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca
     6901 ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct
     6961 tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg
     7021 ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat ggcttattcc
     7081 tccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca
     7141 tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg caatgcagcc
     7201 tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc
     7261 ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc
     7321 tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc
     7381 tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag
     7441 aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca
     7501 aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa
     7561 gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg
     7621 acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg
     7681 attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt
     7741 tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc
     7801 acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg
     7861 gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc
     7921 acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc gtgcatcccc
     7981 agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat taaagtcatg
     8041 gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc attgatcaca
     8101 ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc tacgtcacga
     8161 atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg ctgagcttca
     8221 atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg gccagggcgg
     8281 cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg
     8341 accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac ttcctggaag
     8401 gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag cgggatggag
     8461 tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc gctacaggcc
     8521 atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa gcccgcgccc
     8581 tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca
     8641 ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg ttccaggaaa
     8701 tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc cgaacttgcc
     8761 atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg
     8821 cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg
     8881 ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt tggtagtagt
     8941 tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt
     9001 tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag
     9061 gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg agcgaggttt
     9121 cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac acagaaagta
     9181 tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt agtgcatagt
     9241 tgagtatcga tcacagtttg cgttttgtcc aaatattact gtttatttat acagtaaact
     9301 tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc cggcgcacta
     9361 agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt
     9421 atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct ggcaacaggc
     9481 agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg
     9541 tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg cttgatgctg
     9601 ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg ggtaaccgct
     9661 ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta tcttatccgc
     9721 cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct gccgctaaac
     9781 aactccccat tcctggcgta tatctggcta acattcatca atgtgataga ttcctctccc
     9841 gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca tccgtgataa
     9901 tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg gccgtcggtc
     9961 agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc
    10021 gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt gtctcacagc
    10081 tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc gtcggaatgt
    10141 ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg gtattgattg
    10201 ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc
    10261 cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta aacatgggga
    10321 ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg atgtggaact
    10381 ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc agtatggccc
    10441 gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt accagttata
    10501 aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt ggcggtctgg
    10561 ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg ttagggctga
    10621 gcagtatgac ggtcagtatt ttgtttattc tgccgattcc ggcagcgttt tttggcgcat
    10681 ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg gttatctgct
    10741 gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg aatgtctgga
    10801 cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt cctctggcga
    10861 ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg ctggtcggcg
    10921 gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg
    10981 gtcagggcag cctggggttg ttgatgacct taatgggatt gttgatcgtg ctgtgctggc
    11041 taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac gtcaccgcag
    11101 catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca tcacccgcat
    11161 atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc tgtctggcgt
    11221 ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt cacagcgcgg
    11281 ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct caatcgagat
    11341 acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt tcccccacag
    11401 ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa gatagggaat
    11461 gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg caggttgcag
    11521 agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa tgacctgact
    11581 tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt tacagcaaac
    11641 agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat
    11701 tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta gtgccagagg
    11761 atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc cgcctggtgc
    11821 cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt acgaaaccac
    11881 gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc tttgcggcta
    11941 tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata ttaagctaat
    12001 gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt tttcattgcg
    12061 taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca acaacaacgc
    12121 cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa gcccaacccc
    12181 cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct cattagccag
    12241 cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg ctcctttgca
    12301 atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc ccgtttcctg
    12361 atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca aaataaaatc
    12421 agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat ccacttttca
    12481 ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga caaaacggct
    12541 gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac cgtggagaaa
    12601 aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact ggccattgct
    12661 gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg cattcgcggc
    12721 gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg tatcggcatc
    12781 gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg ttcgctggtt
    12841 taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt cagaacttct
    12901 gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag tcacgtacca
    12961 ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc ggccgtgcat
    13021 ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa ggacgtggac
    13081 ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
    13141 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    13201 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    13261 gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct tgtgtacgct
    13321 ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag ctggcggcgc
    13381 atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg
    13441 cgccccggc
//
LOCUS       DAZPLM010000108        13398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPLM010000108 DAZPLM010000000
VERSION     DAZPLM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.108"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(495..1586)
                     /gene="ychF"
                     /locus_tag="ACHVC7_002636"
     CDS             complement(495..1586)
                     /gene="ychF"
                     /locus_tag="ACHVC7_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR2116654.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1703..2287)
                     /gene="pth"
                     /locus_tag="ACHVC7_002637"
     CDS             complement(1703..2287)
                     /gene="pth"
                     /locus_tag="ACHVC7_002637"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR2116655.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2564..2842
                     /gene="ychH"
                     /locus_tag="ACHVC7_002638"
     CDS             2564..2842
                     /gene="ychH"
                     /locus_tag="ACHVC7_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR2116656.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2897..4576)
                     /gene="dauA"
                     /locus_tag="ACHVC7_002639"
     CDS             complement(2897..4576)
                     /gene="dauA"
                     /locus_tag="ACHVC7_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR2116657.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4700..5647)
                     /gene="prs"
                     /locus_tag="ACHVC7_002640"
     CDS             complement(4700..5647)
                     /gene="prs"
                     /locus_tag="ACHVC7_002640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR2116658.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5798..6649)
                     /gene="ispE"
                     /locus_tag="ACHVC7_002641"
     CDS             complement(5798..6649)
                     /gene="ispE"
                     /locus_tag="ACHVC7_002641"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR2116659.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6649..7272)
                     /gene="lolB"
                     /locus_tag="ACHVC7_002642"
     CDS             complement(6649..7272)
                     /gene="lolB"
                     /locus_tag="ACHVC7_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR2116660.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7486..8742
                     /gene="hemA"
                     /locus_tag="ACHVC7_002643"
     CDS             7486..8742
                     /gene="hemA"
                     /locus_tag="ACHVC7_002643"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR2116661.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8784..9866
                     /gene="prfA"
                     /locus_tag="ACHVC7_002644"
     CDS             8784..9866
                     /gene="prfA"
                     /locus_tag="ACHVC7_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR2116662.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9866..10699
                     /gene="prmC"
                     /locus_tag="ACHVC7_002645"
     CDS             9866..10699
                     /gene="prmC"
                     /locus_tag="ACHVC7_002645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR2116663.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10696..11088
                     /gene="sirB2"
                     /locus_tag="ACHVC7_002646"
     CDS             10696..11088
                     /gene="sirB2"
                     /locus_tag="ACHVC7_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR2116664.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11092..11901
                     /gene="sirB1"
                     /locus_tag="ACHVC7_002647"
     CDS             11092..11901
                     /gene="sirB1"
                     /locus_tag="ACHVC7_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR2116665.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11937..12791
                     /gene="kdsA"
                     /locus_tag="ACHVC7_002648"
     CDS             11937..12791
                     /gene="kdsA"
                     /locus_tag="ACHVC7_002648"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR2116666.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12940..13047)
                     /locus_tag="ACHVC7_002649"
     CDS             complement(12940..13047)
                     /locus_tag="ACHVC7_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2116667.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3341 a         3362 c         3456 g         3239 t
ORIGIN      
        1 tatctgcata acaaaaacaa tatgcgccat ttttgcatta ctaataagaa aaagcaaaaa
       61 acatcgatat tcatttttgg tcaatgggtt ttctggccta tcgttgttat tgtgctactg
      121 gttatcattt tcatcttgcc aggatcgcac gtaatcacgt tattcgggga ctttatctgc
      181 gggatattac ccttcaggta agtccttttc tcgccagcat attggagcgt gatcaatatt
      241 gatcagctgt gaagggggtg atgtgtagtt gaacaaaaac ttacgttata tcactactaa
      301 tgagatatcc gtcatctgac actgatgtct ttaattattc tcgtggttca ctgtaggcaa
      361 taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat gaaaaatcca
      421 cgcaattgcg tggattttat atacttttgc gctcttcatg agatttagcg aaacctcatg
      481 agacaataaa ttaattagac gttgaaaagg aagttcatca catcgccatc tttaacgatg
      541 taatctttac cttctgcacg cattttgcct gcttctttcg cgccttgttc acctttgtaa
      601 gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc aaaatcagta
      661 tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca tgcacgcact
      721 tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc gacacggatc
      781 acacggttca ggcccggttc ttccagccca agctcctgca taaactcgtc acgttcttcg
      841 tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaaccac aacagaacct
      901 tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa accgtcttcg
      961 ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta acgaatagcc
     1021 gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc cagctggggc
     1081 aggcattttt ctaggaccgc cagctcagct ttcgcgtctt tatcgccacc tttggctttc
     1141 ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc cagttcggtg
     1201 ttgataactt caatatcgtc agccgggtta actttgccgg aaacgtgaat gatgttgtca
     1261 ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt ggtcaggaac
     1321 tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat atcgacaaat
     1381 tccatggtcg tgggaagcgt acgttgcggt tttacgattt cagccagttg atccaggcga
     1441 gggtcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg aaagttggcc
     1501 gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac gttgggcaaa
     1561 ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta ataatcaatc
     1621 tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca cggcactcgg
     1681 caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg ttcgttgctt
     1741 tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg atggcttcat
     1801 caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca actttatttt
     1861 tatcgccagg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta ttacccaatt
     1921 tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaat ttaaatttgg
     1981 cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc ggattaatgc
     2041 ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat gtagtcggga
     2101 ctaacaggcg gacatcttcg cctccaagag tgactcgcga agtataacca aagaatttag
     2161 cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac caggcaccag
     2221 cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca atcaatttaa
     2281 tcgtcacgtt ttttgtcctg agtgtgtaca taactggcgc gtagtttact ggttgcggcc
     2341 ccgcttgaca aaaaactgcg tatcaaatgc agataacgta ataattgcct gagtggacta
     2401 ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa
     2461 ttgtgatcac gcccgcacat aacccacagg gtgttgtcta tactttacac ataaggaaga
     2521 ggggtattcc ctgttacaac ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc
     2581 ttcgttactc ggtaacgtgc tcatggggtt ggggctggtg gtaatggtgg tcggcgtggg
     2641 gtattcaatc ctcaaccagt taccacagtt taatatgccc cagtatttcg cacatggtgc
     2701 agtgctaagt attttcgtcg gtgccattct ctggctggcg ggtgcccgtg ttggcgggca
     2761 tgaacaggtg tgcgaccgtt actggtgggt tcgtcactat gacaaacgtt gccgccgtag
     2821 cgataatcgc cgtcatagct aacaaataat gcagtttgct gaccagtcag tttgcgctga
     2881 ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg acgattcggg aagaacgcga
     2941 ggcgtcccgg gatcggttga atgcctgcgc gagccatagt gcgcagtggc tggaattcca
     3001 cgttgcacac gcgcagttca catccttcgg gcagacgctt cacaaaacgc tggaacgcat
     3061 caagaccacc agcatcaagt accggaacgg catcccactt cagaatcaca atccgtttgc
     3121 cttcaagacg tgactccagg tccgtgaaca agccttcagc agcagcaaaa aacagcgggc
     3181 caataacgcg cagtaccaga acatcgtctg gaacatctac gactaccggt gccaggcgag
     3241 tcatacgtgc gatacgacgc ataaacagca gcgatgccag cacgatcccc acgctgatgg
     3301 caataaccat atcaaacagc acggtcagcg acatgcacag cagcatgaca atgatgtcat
     3361 ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc ttcactcatg ttccacgcca
     3421 ccatcaacag cagggctgcc atagcggaaa gcggcagcca ggagagcagc ggtgccagta
     3481 ccagcagggc aagaataacc agaatagagt ggatcaccgc cgagataggg gaagttgccc
     3541 cggcacggac gttagcggca gaacgcgcga tggcagctgt agcggtaata ccaccaaaga
     3601 acggagcgat aatattcccc agtccctgtc caaccagttc gctgttcgcc ttgtgtttcg
     3661 tcccggtcat accatccagt accacggcgc agagcagaga ttcgattgcg ccgagcattg
     3721 ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc gtgaattctg
     3781 aattaggcag atcccacggc agcaccagtt gcggcagcag ttgcggaata ccgttaccct
     3841 gagaaccatc ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga
     3901 gcaggttaac aatccccatc accgcgcaac cagccagcaa ggccggaagg tgaccgggta
     3961 aacgaatgcc cagacgcggc caaaaaacaa gaatacctag cgtcacaatg ccaatggcag
     4021 catcacccac attaatggtc ggcagcgcca taaataatgc gccgactttt tgtagataat
     4081 gttccgggac atgggccatt tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg
     4141 tgatcccgat acccgaggtg aaacctaagg tgacggaaac cggaatatac tcaatcaggc
     4201 gaccaaagcg tgccagaccc atcagaatca aaaagatccc cgacagcaag gtcgcaacca
     4261 gcagtcctgc cagtccaaac tgttgcgaca cgggatagag aattaccaca aatgccgcag
     4321 tcggaccgga aacgctaaag cgtgacccac ccgtcagagc aatgacaatc cccgcaacag
     4381 ctgcggtata taaaccgtac tggggtgcca caccactacc aatagccaac gccatcgcca
     4441 gcgggatagc aataatcccg acggttatcc cggcaatcag gtcacgggta aaccgtgcgg
     4501 cagtatattt ttctttccag caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg
     4561 aaaatatttt gttcacaata atgtttcatc cgtgagcgca tcatctgtca actaaatggc
     4621 aggtgaagga ggcataggtc atacaaatgg atattacaga caaaaaaacc cgccgcagcg
     4681 ggtctttgag cgggttcgat tagtgttcga acatggcaga gatcgattct tcgttgctga
     4741 tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg ttcggcagtg
     4801 atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca attacagagt
     4861 tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac gcaaatacac
     4921 gtttagcacc acgttctttc agagcttcag cagctttaca cagcgtaccg ccagtgtcga
     4981 tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata tgcatcacct
     5041 gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc catatcggta tcgttcagca
     5101 gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc acaattgggt
     5161 tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat acgttatcaa
     5221 ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc agcacacggt
     5281 caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt acacgagcgg
     5341 aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg gtgatacgac
     5401 ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg ttgtcgttag
     5461 taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt tcgttgattt
     5521 gtacactgac ttcgccgtcg ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca
     5581 ggcggttggc aatacgttgt gctagttccg gggtggcgtt accagcaaaa agcttcatat
     5641 caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt
     5701 gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca acgtctggaa
     5761 caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct ctgtgcaatg
     5821 gggaaagatt aacacctttc gccacaaagc cattgagcca ttccggggct tgctctagca
     5881 cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct gtcccagtca
     5941 ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt
     6001 ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt tctattgacc
     6061 tttttggcgt attgcgcggg agttcaggat ctttaaaaat cactggtgtc ggaatactta
     6121 caccagggtg cgccaccaga taccacttct ctggcggatc caccggcgtt agtatttcac
     6181 caacgccttc ggcaaacgcg gcatgccccc gaacaaagac aggaacatct gcgcccagcg
     6241 tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga tgatttaatg
     6301 ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc atcggcaaac
     6361 gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag
     6421 ttttcatcaa caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa
     6481 cgggcgttaa cagacgaata tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt
     6541 aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa
     6601 tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt atttcactat
     6661 ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca tattggctgg
     6721 catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt ttttgccatt
     6781 ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc
     6841 acccggtaaa cctaaaatcc attggcgcaa gctgttgagc ggaattggca ttccggtcaa
     6901 tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat tgtcgactaa
     6961 ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg ggttagtgag
     7021 cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt acactttttg
     7081 ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat tgcgcacgtc
     7141 ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt
     7201 aacggaacag gcagtgagca caagagcagc cagcggtagc aggcggataa gacgaaaatc
     7261 gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac ccttaatgct
     7321 agcgttaccg tccgctatcg tctatgttca agttgtctta attgccagaa tctaacggct
     7381 ttcggcaatt actccaaaag ggggcgctct cttttattga tcttacgcat cctgtatgat
     7441 gcaagcagac taaccatatc aacgttggta ttatttcccg cagacatgac ccttttagca
     7501 ctcggtatca accataaaac ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg
     7561 gataagctcg atcaggcgct tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg
     7621 gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg ttgaagagca ggataacctg
     7681 caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga agatctgcgt
     7741 aaaagcctct actggcatca ggataacgac gcggttagcc atttaatgcg tgttgccagc
     7801 ggcctggatt cactggttct tggggagccg cagatcctcg gtcaggttaa aaaagcgttt
     7861 gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct
     7921 ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg gtgccagtgc tgtgtctgtc
     7981 gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac ggtcacagtg
     8041 ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa
     8101 gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaattct ggcagatgaa
     8161 gttggcgcgg aagtgattgc cctgagtgag atcgacgaac gtctgcgcga agccgatatc
     8221 atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt ggagcgcgca
     8281 ttaaaaagtc gtcgtaacca accaatgctg ttggtggata ttgccgttcc gcgcgatgtt
     8341 gagccggaag ttggcaaact ggcgaatgct tatctttata gtgtggacga tctgcaaagc
     8401 atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc
     8461 gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt
     8521 cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt taaccgccaa agcgttagcg
     8581 gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg gaaactgact
     8641 aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga cggggataac
     8701 gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat cattttcttt
     8761 ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg gaagccctgc
     8821 atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc gccgaccagg
     8881 aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta
     8941 ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc gatgatcctg
     9001 aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg
     9061 aacagcaatt acaggttctg ttactgccaa aagatcctga tgacgaacgt aacgccttcc
     9121 tcgaagtccg agccggaacc ggcggcgacg aagcggcgct gttcgcgggc gatctgttcc
     9181 gtatgtacag ccgttatgcc gaagcccgcc gctggcgggt agaaatcatg agcgccagcg
     9241 agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg
     9301 gtcgtctgaa atttgaatcc ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc
     9361 agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg cctgacgcag
     9421 aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg tcaggggcgg
     9481 gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg ccgaccggga
     9541 ttgttgttga atgtcaggac gaacgttcac aacataaaaa caaagctaaa gcactttctg
     9601 tacttggtgc tcgtatccac gctgcagaaa tggcaaaacg gcaacaggcc gaagcgtcta
     9661 cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact tacaacttcc
     9721 cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgtctg gatgaagtga
     9781 tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag gccgaccaac
     9841 tggcggcgtt gtccgagcag gaataatgga atatcaatac tggttacgtg aagcaataag
     9901 ccaacttcag gcgagcgaaa gcccacgccg tgacgctgaa atcttattgg agtatgtcac
     9961 tggcaggggg cggactttta ttctggcctt tggtgaaacg cagctgactg acgaacaatg
    10021 tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg ctcatttaac
    10081 cggggtgcga gaattctggt cgttgccgtt atttgtttcg ccagcgacct taattccgcg
    10141 cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac aaccttgccg
    10201 tattctcgat ctcgggacgg gtaccggggc gattgcgctt gcgctggcta gcgagcgccc
    10261 ggactgcgaa attatcgctg tagatcgtat gcctgatgct gtctccctgg cacaacgtaa
    10321 tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt ttagcgcgct
    10381 agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg agcaggaccc
    10441 acatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg cggcagacag
    10501 tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg tatccggcgg
    10561 ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacaag catttatcct
    10621 cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc gcgtaacgct
    10681 cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct tattagtatc
    10741 gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa gcatccacag
    10801 gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacggtgtt attgttaagc
    10861 ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc acagtggctg
    10921 actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc actcgattat
    10981 cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg
    11041 tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt catgagatcg
    11101 ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa
    11161 gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg tctcgttagt
    11221 ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagctgga aaaactgatc
    11281 gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gcgtttatcg tctttccgat
    11341 gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc attaggtgcg
    11401 gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat tttccctacg
    11461 cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa tccttttaac
    11521 ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat cagcccgtcg
    11581 gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg
    11641 ttggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc gttacgcacc
    11701 agcgaagctt tattacaatt caacccggaa gatccctatg aaattcgcga tcgcgggttg
    11761 atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa
    11821 cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa catcgcgcat
    11881 aaacatattg tgctgcatta attaatcgac attttactca agattaaggc gatcctatga
    11941 aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgtcg ttcgtactgt
    12001 ttggtggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc gagcactacg
    12061 taaccgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt gacaaagcca
    12121 accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg aaaatcttcc
    12181 aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg
    12241 cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga
    12301 ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt
    12361 ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa
    12421 aagtgattct ttgcgatcgc ggtgctaact tcggctatga caacctggtt gtcgatatgc
    12481 tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc
    12541 acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg
    12601 ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt gaagcgcatc
    12661 cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct aaactggaac
    12721 cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa gaactggata
    12781 ccagcaagta atcttttttg cttgaaaaat aaagtcttag cgttctgcgt taagactttt
    12841 ttcatgggtg ccagatacaa aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc
    12901 gcatattgcc atcacggcag cctgacgccc gttttcacct tacttccggt tacgccacca
    12961 gccgacaatc gctgcggtaa taattcccgc caggatcggt gctgccaggt cgtgccagaa
    13021 aatcatggca aactgcgcga gcgtcatata gccgccttgt tgtaatgaca acattttgcg
    13081 gctattcttg aattgttctg gttcaagatt agcccccgtt ctgttgtcag gttaaacctc
    13141 tcaacgtgcg ggggttttct ctttccagca accaatgcca ccagggataa agcccccgca
    13201 acattgcgcc tcaccggata acgccggctt gatgtggata ctacgtctca attcatcttc
    13261 acttcatccc tgaaatgttt gcaatgaaga gtgcattccg gtttttcaac agctgttaca
    13321 gtcgtttcat gagtgctctg gatgatgctt ccagctcggg ttgccaatat ttacttgtgg
    13381 aggagataaa gacaaaaa
//
LOCUS       DAZPLM010000109        13372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPLM010000109 DAZPLM010000000
VERSION     DAZPLM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.109"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHVC7_002650"
     CDS             complement(<1..312)
                     /locus_tag="ACHVC7_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR2116668.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHVC7_002651"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHVC7_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR2116669.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHVC7_002652"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHVC7_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR2116670.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHVC7_002653"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHVC7_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR2116671.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHVC7_002654"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHVC7_002654"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR2116672.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHVC7_002655"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHVC7_002655"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR2116673.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHVC7_002656"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHVC7_002656"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR2116674.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHVC7_002657"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHVC7_002657"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR2116675.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHVC7_002658"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHVC7_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR2116676.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHVC7_002659"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHVC7_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR2116677.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHVC7_002660"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHVC7_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR2116678.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHVC7_002661"
     CDS             complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHVC7_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR2116679.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3519 a         3405 c         3014 g         3434 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gc
//
LOCUS       DAZPLM010000110        13262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPLM010000110 DAZPLM010000000
VERSION     DAZPLM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.110"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHVC7_002662"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHVC7_002662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR2116680.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHVC7_002663"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHVC7_002663"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR2116681.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHVC7_002664"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHVC7_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR2116682.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHVC7_002665"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHVC7_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR2116683.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHVC7_002666"
     CDS             complement(4036..5568)
                     /locus_tag="ACHVC7_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR2116684.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHVC7_002667"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHVC7_002667"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR2116685.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHVC7_002668"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHVC7_002668"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR2116686.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHVC7_002669"
     CDS             complement(8663..10399)
                     /locus_tag="ACHVC7_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR2116687.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHVC7_002670"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHVC7_002670"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR2116688.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHVC7_002671"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHVC7_002671"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR2116689.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHVC7_002672"
     CDS             complement(12121..12855)
                     /locus_tag="ACHVC7_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR2116690.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13262
                     /locus_tag="ACHVC7_002673"
     CDS             13056..>13262
                     /locus_tag="ACHVC7_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR2116691.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3367 a         3373 c         3488 g         3034 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aatcagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 tg
//
LOCUS       DAZPLM010000111        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPLM010000111 DAZPLM010000000
VERSION     DAZPLM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.111"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHVC7_002674"
     CDS             <1..191
                     /locus_tag="ACHVC7_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116692.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHVC7_002675"
     CDS             191..1393
                     /locus_tag="ACHVC7_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR2116693.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHVC7_002676"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHVC7_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR2116694.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHVC7_002677"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHVC7_002677"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR2116695.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHVC7_002678"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHVC7_002678"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR2116696.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHVC7_002679"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHVC7_002679"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR2116697.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHVC7_002680"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHVC7_002680"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR2116698.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHVC7_002681"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHVC7_002681"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR2116699.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHVC7_002682"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHVC7_002682"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR2116700.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPLM010000112        12938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPLM010000112 DAZPLM010000000
VERSION     DAZPLM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.112"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..324
                     /locus_tag="ACHVC7_002683"
     CDS             <1..324
                     /locus_tag="ACHVC7_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFR2116701.1"
                     /translation="IEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKD
                     PESKAPKYIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVE
                     EMMSH"
     gene            667..1197
                     /locus_tag="ACHVC7_002684"
                     /pseudo
     CDS             667..1197
                     /locus_tag="ACHVC7_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1767..1856)
                     /locus_tag="ACHVC7_002685"
     tRNA            complement(1767..1856)
                     /locus_tag="ACHVC7_002685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1820..1822),aa:Ser,seq:cga)
     gene            1950..2747
                     /gene="mtfA"
                     /locus_tag="ACHVC7_002686"
     CDS             1950..2747
                     /gene="mtfA"
                     /locus_tag="ACHVC7_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR2116702.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2848..2923
                     /locus_tag="ACHVC7_002687"
     tRNA            2848..2923
                     /locus_tag="ACHVC7_002687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2881..2883,aa:Asn,seq:gtt)
     gene            3237..10302
                     /gene="yeeJ"
                     /locus_tag="ACHVC7_002688"
                     /pseudo
     CDS             3237..10302
                     /gene="yeeJ"
                     /locus_tag="ACHVC7_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(10564..11616)
                     /locus_tag="ACHVC7_002689"
     CDS             complement(10564..11616)
                     /locus_tag="ACHVC7_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR2116703.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11931..>12938
                     /gene="shiA"
                     /locus_tag="ACHVC7_002690"
     CDS             11931..>12938
                     /gene="shiA"
                     /locus_tag="ACHVC7_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR2116704.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         3605 a         3048 c         2991 g         3294 t
ORIGIN      
        1 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
       61 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
      121 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
      181 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
      241 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
      301 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
      361 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
      421 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
      481 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
      541 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
      601 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
      661 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
      721 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
      781 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
      841 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
      901 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
      961 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     1021 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     1081 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     1141 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     1201 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     1261 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     1321 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     1381 tatgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     1441 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     1501 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     1561 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     1621 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     1681 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     1741 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     1801 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     1861 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgtcc gtgcaatttt
     1921 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     1981 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     2041 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     2101 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     2161 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     2221 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     2281 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     2341 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     2401 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     2461 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     2521 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     2581 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     2641 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     2701 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     2761 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     2821 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     2881 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     2941 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     3001 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     3061 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     3121 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     3181 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     3241 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     3301 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     3361 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     3421 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     3481 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     3541 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     3601 gtgaaaaaaa attaaccccg ccgccgggta atagcagtga caacctcgag caacagatag
     3661 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     3721 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     3781 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     3841 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     3901 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     3961 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     4021 actcccgcgc cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     4081 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     4141 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     4201 gcggtaaact ggtctatgaa cagtattatg gcgatgaagt ggccctgttc gataaagacg
     4261 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     4321 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     4381 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     4441 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     4501 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     4561 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     4621 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     4681 atattctggt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     4741 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     4801 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     4861 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     4921 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     4981 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     5041 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
     5101 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
     5161 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
     5221 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
     5281 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
     5341 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
     5401 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
     5461 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
     5521 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
     5581 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
     5641 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
     5701 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
     5761 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
     5821 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
     5881 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
     5941 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
     6001 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
     6061 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
     6121 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
     6181 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
     6241 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtcg ggctaacagc
     6301 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
     6361 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
     6421 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
     6481 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
     6541 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
     6601 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
     6661 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
     6721 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
     6781 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
     6841 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
     6901 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
     6961 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
     7021 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
     7081 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
     7141 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
     7201 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
     7261 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
     7321 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
     7381 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
     7441 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
     7501 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
     7561 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
     7621 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
     7681 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
     7741 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
     7801 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
     7861 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
     7921 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
     7981 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
     8041 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
     8101 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
     8161 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
     8221 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaggg cgcgaccatg
     8281 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
     8341 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
     8401 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
     8461 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
     8521 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
     8581 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
     8641 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
     8701 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
     8761 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
     8821 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
     8881 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
     8941 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
     9001 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
     9061 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
     9121 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
     9181 gaggatggca gtggtaacct gatcgaaggt ctcactgtgt actttgcctt aaaaagcggc
     9241 tctgccacat taacgtcatt aacagcggtg accgatcaaa acggaatcgc gacaacaagc
     9301 gtgaaaggag cgatgacagg tagcgtcacg gtaagcgcag tcacgaccgc tggtggaatg
     9361 caaacagtag atataacgct ggtggcaggc ccagcagacg cctcaaagtc agtccttaag
     9421 aacaatcggt catcattgaa aggagacttt accgatagtg ctgagctaca tcttgttctg
     9481 cacgatatat caggcaatcc gatcaaagtt tctgaagggc tggaatttgt acagtcaggt
     9541 accaacgtgc cctatgtgca agttagtgca attgactaca gtaaaaattt ctcaggcgag
     9601 tacaaagcca ctgttacagg aggcggagag ggtatcacaa cgctgatccc tgtattgaat
     9661 ggtgttcatc aagctggtct gagtaccaca atacaattca ctcgcgcaga agacaaaata
     9721 atgagcggta cagtatcagt caatggtact gacctaccga caactacatt cccttcgcag
     9781 gggttcaccg gggcgtatta tcagttgaat aatgacaact ttgccccagg aaaaacggcg
     9841 gctgattatg agttttcaag ctctgcctcc tgggtcgatg ttgatgctac cggtaaagtg
     9901 acatttaaaa atgtcggcag caattcggaa aggattacgg cgacaccaaa atcaggaggc
     9961 cctagctatg tatacgaaat ccgtgtgaag agttggtggg tgaacgccgg cgaggctttc
    10021 atgatataca gccttgctga aaatttttgc agcagcaatg gctacacgct ccccagagca
    10081 aactatttaa accacagtag ttcccgaggc atcgggtcac tgtacagtga atggggagat
    10141 atggggcatt acacgactga agctggtttt caatcaaata tgtattggtc atctagtccc
    10201 gcaaactcaa gcgaacaata cgtagtttcc ctggcaacag gtgatcaaag cgtatttgaa
    10261 aagcttgggt ttgcttatgc gacatgttat aaaaacctct gattttccgt tataatttct
    10321 taaagagtgc tccattcgga gctctctttt ttacattccc tctatagagc ttgcccttct
    10381 ctcacagaaa atgaataaat aaaaatgcgg caccgccaga attgcgttcg atgtgactat
    10441 gatcgtatcc ggtgagtact gctttgattc aaaggctggt tttctgtgat gattgaccag
    10501 cctcagagta ttgatgacac caccgttcat aacagatcgg cattactatg cataagtact
    10561 gtctcatatt ctcgccaaaa cactttttaa tgcctttgcg aattcatcca gacttatatt
    10621 gtcaattttt tgcccaacga cgactgactc cgagacatcg gccattgcgg gccatcgcgt
    10681 tttgtaatca gcataaattg caacaacagg ggtattatag gcacttgcca tatgtaatat
    10741 tgaggtgtca ggagttattg cgatataagc atcttttatg attgctgctg aacgtttaat
    10801 cgagggtggt aaggataaac ggtagacatt attataacaa tccactaatg ccctggcttt
    10861 atcttcacct ttgggtccat gaacgatcac tattggtata tctgtttcac tttgaatttt
    10921 tgctattaga ttttctgcaa tcgataatga aaatgtacgt tcctgcgagc taccttctaa
    10981 attgaacgca atgtaagatc ctaacgatcg catttcctcg cgcacctcat cgagtacatc
    11041 ctcacttagt ggcaattcat atattggcct gactgctgga aaacccgcct cacgcatcat
    11101 aaatgcccat gtcataggta cgggagcccg gagtttctga tccatacggg acgcgttctt
    11161 gcacaaaggg gagtagcaat tcatggttat accaacaacc tgaaaattcg tttttgcttt
    11221 caactgactg ataaataaca tcgttttcag gttcttttta cgcatcccct caatacaaag
    11281 atccggcgta ccgtattgct gtgttatgtt ctttgctaaa tcttttattt cttttaatgt
    11341 tgcgtgatcc tgcatagtca ttgtgactaa tgttaattta gtctgttcaa gtttaagcgc
    11401 attaaagact tctaaattaa ttgtcgacgt tacaattaaa agatgcttaa ttttatgcaa
    11461 ttcaagcgcc cgaataacag gaaagatggc catagcatcg ccaatctgat cgggaatatg
    11521 gatgacaaca aagtctgttt tttcaatatt gaaattataa gctttataat cgtagtaact
    11581 aaatgcaata cgtctcaaca atgatgctaa aaacatacct aacctcgcct ccctactggt
    11641 tataatgcaa tgcagtctat cagactcatc agggtgccat tttgtgcata tgcggacttt
    11701 tatgtttcat atctctaacc tgtgggtcct ctgcttaatc cttaaacaac accagcaact
    11761 cctgcgcttt catcttccat cgaatttttc atgttgccgc taatcagcca taaaatcatt
    11821 tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg
    11881 agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca
    11941 cgctcatctc cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg
    12001 cgttaggcag cttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatca
    12061 ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc
    12121 tcgccgcatt tgctaccttt ggcgtcggat tccttttccg tccgctcggc ggtgtcattt
    12181 tcggtcactt tggcgaccga ctgggacgta agcgcatgtt aatgctgacc gtctggatga
    12241 tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg
    12301 cacctatttt gctggtgaca ctgcgtgtca ttcagggatt tgccgtcggc ggcgaatggg
    12361 gaggcgcggc gttgctttcc gttgaaagtg caccgaaaaa taaaaaagcc ttttacagta
    12421 gcggtgtaca agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga
    12481 tcagtatgat gacgactgac gaacagtttt taagctgggg ctggcgcatt cctttcctgt
    12541 ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg
    12601 aatttgaaca acagcaacat tatcaagctg ccgcgaaaaa acgcatcccg gttatcgaag
    12661 cgctgttacg acatcccggt gctttcctga agattattgc gctacgactg tgcgaattgc
    12721 tgacgatgta catcgttact gcctttgcac ttaattattc aacccagaat atggggctac
    12781 cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc
    12841 cctgttttgc ctggcttgcc gatcgttttg gtcgccgtag ggtttatatc acaggtgcgt
    12901 taatcggaac gttgagcgca tttcctttct ttatggcg
//
LOCUS       DAZPLM010000113        12917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPLM010000113 DAZPLM010000000
VERSION     DAZPLM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.113"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            7..324
                     /locus_tag="ACHVC7_002691"
     CDS             7..324
                     /locus_tag="ACHVC7_002691"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR2116705.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            393..722
                     /gene="yjdP"
                     /locus_tag="ACHVC7_002692"
     CDS             393..722
                     /gene="yjdP"
                     /locus_tag="ACHVC7_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR2116706.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(869..1627)
                     /gene="phnP"
                     /locus_tag="ACHVC7_002693"
     CDS             complement(869..1627)
                     /gene="phnP"
                     /locus_tag="ACHVC7_002693"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR2116707.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1629..2063)
                     /gene="phnO"
                     /locus_tag="ACHVC7_002694"
     CDS             complement(1629..2063)
                     /gene="phnO"
                     /locus_tag="ACHVC7_002694"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR2116708.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2050..2607)
                     /gene="phnN"
                     /locus_tag="ACHVC7_002695"
     CDS             complement(2050..2607)
                     /gene="phnN"
                     /locus_tag="ACHVC7_002695"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR2116709.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2607..3742)
                     /gene="phnM"
                     /locus_tag="ACHVC7_002696"
                     /pseudo
     CDS             complement(2607..3742)
                     /gene="phnM"
                     /locus_tag="ACHVC7_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
     gene            complement(3739..4419)
                     /gene="phnL"
                     /locus_tag="ACHVC7_002697"
     CDS             complement(3739..4419)
                     /gene="phnL"
                     /locus_tag="ACHVC7_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR2116710.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(4530..5288)
                     /gene="phnK"
                     /locus_tag="ACHVC7_002698"
     CDS             complement(4530..5288)
                     /gene="phnK"
                     /locus_tag="ACHVC7_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR2116711.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5285..6130)
                     /gene="phnJ"
                     /locus_tag="ACHVC7_002699"
     CDS             complement(5285..6130)
                     /gene="phnJ"
                     /locus_tag="ACHVC7_002699"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR2116712.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6123..7187)
                     /gene="phnI"
                     /locus_tag="ACHVC7_002700"
     CDS             complement(6123..7187)
                     /gene="phnI"
                     /locus_tag="ACHVC7_002700"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR2116713.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7187..7771)
                     /gene="phnH"
                     /locus_tag="ACHVC7_002701"
     CDS             complement(7187..7771)
                     /gene="phnH"
                     /locus_tag="ACHVC7_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR2116714.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7768..8220)
                     /gene="phnG"
                     /locus_tag="ACHVC7_002702"
     CDS             complement(7768..8220)
                     /gene="phnG"
                     /locus_tag="ACHVC7_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR2116715.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8221..8946)
                     /gene="phnF"
                     /locus_tag="ACHVC7_002703"
     CDS             complement(8221..8946)
                     /gene="phnF"
                     /locus_tag="ACHVC7_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR2116716.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8967..9746)
                     /gene="phnE"
                     /locus_tag="ACHVC7_002704"
     CDS             complement(8967..9746)
                     /gene="phnE"
                     /locus_tag="ACHVC7_002704"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR2116717.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9872..10888)
                     /gene="phnD"
                     /locus_tag="ACHVC7_002705"
     CDS             complement(9872..10888)
                     /gene="phnD"
                     /locus_tag="ACHVC7_002705"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2116718.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10913..11701)
                     /gene="phnC"
                     /locus_tag="ACHVC7_002706"
     CDS             complement(10913..11701)
                     /gene="phnC"
                     /locus_tag="ACHVC7_002706"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR2116719.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11834..12277)
                     /gene="yjdN"
                     /locus_tag="ACHVC7_002707"
                     /pseudo
     CDS             complement(11834..12277)
                     /gene="yjdN"
                     /locus_tag="ACHVC7_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(12435..12770)
                     /locus_tag="ACHVC7_002708"
     CDS             complement(12435..12770)
                     /locus_tag="ACHVC7_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR2116720.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2560 a         3652 c         3897 g         2808 t
ORIGIN      
        1 ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg gagcaatggg cggtcatgtc
       61 gctggcaccg caggattctg cgttgctgtg ccagcctttt attcatggtc aatgagtgag
      121 gtgattgata aagacaccag gtcgtcagag cgcactgatt atccgcatta tgccagtgaa
      181 gttgcactgg cttttggttc acgagtggtt ggcctcgaac tggcaaaaat gattgtggat
      241 gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac aacgcgtgga ggcgattacg
      301 gcaatagagc agcggagaaa ttgagattca tccactactt gcatggatga gtaatgacta
      361 atgtggatag agtttctttt tgaggttggc taatgaaacg ctttccgcta tttcttttat
      421 tcaccctgct cacgctgtcc accgttccgg cccaggctga tattatcgac gatactattg
      481 gtaatattca gcaggcgatt aatgacgctt ataaccctga ccgtgggcga gactatgaag
      541 attcgcgcga cgacggctgg cagcgtgaag tgagcgacga tcggcgcaga caatatgacg
      601 accgccgccg ccagtttgaa gatcgccgca ggcagctgga cgatcgccag cgccagcttg
      661 atcaggagcg tcggcaactg gaggatgaag agcggagaat ggaagatgag tatgggcgat
      721 gaggttgggt atggagcagg catcgcccct caccccaatg gggagaggga gaaaacgagc
      781 gcaatattca atatcgagca caatcggtcc cctcgcccct ctggggagag ggttagggtg
      841 agggggccag cgcgcacatt ccctctcatc acgccacccc aatctccatc ccatcaaacc
      901 ccacctcaaa ccctgacggt agtgcgtttt ccatcagcca cgcatcaaac tggtggctga
      961 tatgggtcag aatcacccgt ggcgagcgga taacctgatt cagcgcaagc acggtattta
     1021 aatcacagtg attacgcggt gcatccgcgc gcggcgggtg actgcaatcc atcaccatta
     1081 cctgcggctg attattgcgt aaaaatttca gcgttttttc cggcaagcca gcggtgtcag
     1141 acagccacgc cacccggctg tgcgccgttt ccagtaaata gccgaaagtg agttttgagt
     1201 ggttaagcgg cagcggcgtg acctgcaacc cctgcaaatc aaacaccaca aacggttcca
     1261 ccgtgtggct gaaatcaagc agcccgggat gtttaaacag atcgtcgcag ccctgttcat
     1321 ccggcgggcc gtagatcggg atcggatcgc ccacgcccca gcgcagcgga aacagcccct
     1381 ggacgtgatc catatgataa tgcgtcagca aaaactgctg gaacgatccg ggagaccagc
     1441 gatcggtgag atcgtgcagc ccggcgtcga tcagggtgat cgcctcgtta aacttcacta
     1501 cgccgctgca cggttggcgg cgatactgcg gcgagcgccg cgctcggtcg caggccgcgc
     1561 actcgcagcc ccatgccgga acgccctgtg cgccgccggt gccggtgagc gtgagggtca
     1621 gactcatgtt acagcgcctt ggtgaagcgg aagtgactct gctcgtagcc ttctcgcaga
     1681 tagaaacggt gcgcgtcgtg gcgcttcacg ttggtcgaaa gctcggtcat ttcggccccg
     1741 gcctggcggg cttcttcttc tgcccacgcc agcaacttgc taccgacgtt caggccgcgc
     1801 gcctgcggca tcactaccag ttcctgaatt tcgccgatcc agttgacatg atgcagatga
     1861 aactgcaaat gcaggccgat catgccgaca acctcgccat ccagcagcgc cagatgatag
     1921 cgcatgttcg ggtcgcgcag attggcgcta aagcccacgc gaaatgcgtg gtggtcaaac
     1981 tccgcctgtt ttagctcaca aatcagcgcg taaaccgcgt cggtatcata ctgcgtggcc
     2041 gggcgaagct cacaagcagg catggtgttt ctccttctga tgaatcagcg tcagcagcgt
     2101 gtcgaccgac tggcgcaggc tgccgtcatt gttgagcgta tggcaatcct gtggagtata
     2161 gcgggcggcg cgcgccaggc gggcgttaat ttcactggcg ttttcacggc cacggttttc
     2221 caggcgctgg cggaggatct ccggcgaaac ctgtaaacag acgggcagca gcgccgattg
     2281 atagcgcgcc cgcgcctgcg gcagatgggc gcgtgagccg ttgaccagca cgtcgaatcc
     2341 ggcgtgcagc cagaggtcga tctcgacgcc gacgccataa tacagaccgt tagcgtgcca
     2401 gcttaaggcc agtaggttct gccccgcgcg ggtaaaaaac tcctgctcgc tcagagcgat
     2461 atggttttcg cttccggcgc tggcgtcgcg cgtgatgtag cgatgcgcca ccagtagctg
     2521 agtttgttcc cgcaggcgga gttccgccag caggctatct ttcccggagc cggacggccc
     2581 cattaaccaa atcagttttc ccatcatcag aacacccttt taccctgacg ccagacgtgg
     2641 tcgatatgaa tgtgattgcc cttgcgatgc gccagcacca agtccgcgcg tttgccctcg
     2701 ccaatcaccc cgcgatcctg gagattaagc gcctgcgctg gatttttagt caccagcttc
     2761 accgcctgcg gcagcgtaaa gcggttgctc tcgtcatcgg cgacgcgaaa tgccgcatcg
     2821 agcaggctgg cggggtagta gtcggaagag aggatatcca gcaggccaag ctgcgccagt
     2881 tcactggctg ccacgttgcc ggagtgcgag ccgccgcgca caatattcgg cgcgcccatc
     2941 agcacgttca tgccatgctt gcgcgaggct tccgccgctt cgaacgtggt gggaaattcg
     3001 gcgatcacgc tgccaagctg gtgagattca gcaacgtggg cgtgggtgcg tcatcgtggc
     3061 tggcaagcgc aatttttcgc gcgcggcaca gggcggcgat ggattcgcga ttcggctgcg
     3121 accagcgtgc ggcgagcgcc agttgctctt cttcgtactg ctgcatctgc gcatcagtga
     3181 gggagtattt gccctgataa tattcgcgat acttctcgcg attggcgaac tggcgctggc
     3241 ccggcgagtg gtccatcagc gacaccagcg tcaccggctc gcgctgcacc agtttttcaa
     3301 acagcggcag cgtggtgtga tgcggcagtt cgcagcgcag atgcagacgg tgctcggcgc
     3361 ggttgacgcc gcgtttctgc gtctcttcga tggcgttgat catcttctcc agattcacca
     3421 gccgatcgcc gccgtcgcgc acgtcgccaa ttgccacggc atccagtacg gtggtgatgc
     3481 cgctcgccac catcagcgcg tcgtggctgc tcatcgccga gtgggcaggc cagtcaactt
     3541 tcgggcgcgg ggtgaagaat ttatccagat tatcggtatg cagctcaatc agccccggca
     3601 gcagccagcc gccttcgccg tccatcgcct ccggcaggcg gctctggctt tcggcaaagg
     3661 cgcggatttc gccgtcctgc acctccagtg aaccgtgtac cacttcgttt tccagcacca
     3721 atttgacgtt attgataatc atgaagaggc ttccattggg tgcaggcggt cggcaacgtc
     3781 attacgtacg gcttcgtcgt ggaagatgcc gacgatggcc gcgccacggg ctttggcttc
     3841 gtgaatcagc tcgactacgg cggcgctgtt tttggcgtcc agcgaggcgg taggttcgtc
     3901 gagcagcaga atggggtagt cgacgataaa gccgcgggcg atgttgacgc gctgctgttc
     3961 gccgccggag aaggtcgatg gcgccagatg ccacaggcgt tccggcacgt tcaggcgggt
     4021 gagcagacgc gcggctttag cggcgcaggc ttcacgcggt acgccggtat cgagtagcgg
     4081 ctgcatcacc acttccagcg ccgaaatacg cgggatgacg cgcagaaact ggctcaccca
     4141 gccaacggtg gttttgcgga tctccaccac tttgcgcgct ggcgcggtga ccagatcgac
     4201 ccattcgtca ccgtgtttga tctggatttg cccttcgtcg ggcagatagt tggcgtacag
     4261 cgagcgtagc agggttgatt ttccgctgcc ggaatggccg tggagcacca cgcattcgcc
     4321 cgcgttgacg atgagcgagg cgcggttgag gacgggcagg cgcacgccgt tttgctggtg
     4381 caggatgaag gttttactga cgttttgtac gttaatcatt tttcgcctcg tggttcgggt
     4441 gtcggatgcg acgctgacgc gtcttatccg acctacgggg agcgcatttg taggccggat
     4501 aaggcgttta cgccgcatcc ggcaccggct caattctgca aaaccgatga caccagcagc
     4561 tgtgtatacg gatgatgcgg gtcgtcgagc acgcggtcgg ttaacccact ctccaccact
     4621 tgcccctgct tcatcaccag caaacggtcc gccagcaggc gggcgacgcc taaatcgtgg
     4681 gtgacaatca ctaccgcgag gttcagctcc accaccagtc cgcgcagcag gtcgagcagg
     4741 cgggcctgca ccgacacatc cagcccgccg gtcggttcat ccataaacac cagcttcgga
     4801 tgcgtcacca ggttgcgggc aatctgcaaa cgctgctgca taccgccgga aaaggtggtc
     4861 ggcaggtcgt cgatacggtt ggcgggaatc tccacctctt ccagccactt ctgcgcggta
     4921 gcgcgaatat cgccgtaatg tcgcgccccg gtcgccatca gccgctcgcc gatattgccg
     4981 cctgccgaca cctggcggcg caggccgtcg agcggatgct gatgcaccac gccccattcg
     5041 gtgcgcagca ggcgacggcg gtcggcctcg ctcattgcat agagggaacg gttttcatag
     5101 tgaatctccc cctgctgcgg cgtcagacgc gcggagatcg acttcagcag cgtggtcttc
     5161 ccggagccgg attccccgac aatgcccagc acttcccccg gccataaatc aaaagagaca
     5221 tcgctaaagc ctttgcccgg cgcgtaaagg tgggtcaggt tattgaccga aagtaacggt
     5281 tgattcattg gcttttcgcc tcgctctgtt ggcggcaata atcggtatcg gagcagacaa
     5341 acatgcggtt tccggcgtca tccagcacca cttcatcaag atagctgtgg gtcgatccgc
     5401 agatggcgca tggctcatcc cactgctgaa cggtgaacgg gtgatcgtcg aaatcgagac
     5461 tttccacgcg ggtaaacggc ggcaccgcat agatgcgctt ctcgcgccct gcgccgaaca
     5521 gctgcagggc gggcatcatg tccattttcg ggttatcgaa tttcgggatc ggcgacgggt
     5581 ccatcacgta gcgcccgttc accttcaccg gataagcata ggtggtggcg atatgaccga
     5641 agcgggcgat atcttcatac agtttcacct gcatcacgcc gtactcctcc agcgcgtgca
     5701 tagtgcgggt ctccgtttcg cgcggctcga taaagcgcag cggttccggg attggcacct
     5761 ggaagataat gatctgatct tcggtcagcg gcgtttcggg gatgcggtga cgcgtctgga
     5821 taagcgtcgc atcgtccgta cattcagtgg tgtttacccc ggtcacgcgc ttaaagaagt
     5881 tgcgaatcga cacggcgttg gtggtgtcat ccgcaccctg gtcaatcacc ttcaacacgt
     5941 cgctttcgcc aatcacgctg gcggtgagct ggataccacc agttccccag ccgtacggca
     6001 tcggcatctc gcgcccgcca aacggtacct gataaccggg gatcgccacc gcttttaaga
     6061 tggcgcggcg gatcatgcgt ttggtctgct cgtcgaggta ggcaaagttg tagccgctca
     6121 gattagccat ggttctgctc ctgttgcaga cgtttgagta gctccagttc ggcctggaaa
     6181 tcgacgtagt gcgggagttt gaggtgcgag acaaagcctg cggcttcgac gttgtcggca
     6241 tgcgccagca cgaactcttc atcctgcgcc gggcctgtcg cgtgctcgcc gtattccggg
     6301 gcttgcaacg cacggtcaac cagcgccatt gccatcgctt tgcgctcgct catgccgaat
     6361 accagcccgt agccgcgcgt gaagtgcggc ggctcatccg gcgggtcgat aaaaccgttg
     6421 accatttcac actcggtcat cagcagttcg ccgacgttta ccgcaaatcc cagctcttcc
     6481 ggcacaatcg acacgtcgat gtaaccactg cggatctcgc ccgcgaacgg gtggttgcgc
     6541 ccgtaaccgc gctgggtgga gtaagccagc gccagcaaat agccttcgtc gccgcgcatt
     6601 aactgctgca aacgggaaga acgtgaacag gggtaaaccg gcggcgtgcg ggtgatgtca
     6661 tccggctgtg ctccgctatc ctcttcaaac ttcgccagcc cctgacgcgc cagcaggctg
     6721 aaaacgtgcg gcgacggctg ttgttcgctg tcggcggtgg tcagcgtcgg cgcttcgccg
     6781 tttgccagca gagtgaaatc gagcaggcga tgggtgtagt cgtaggttgg gccaagcagc
     6841 tggccgccgg gaatatcttt ataaaccgct gaaatacgcc gttcgagacg catctccgtg
     6901 gtgtcgagcg gctcgcttac cgccagtttc gtcagcgtgg tgcggtaggc gcgtagcagg
     6961 aaaatcgctt cgacgttatc gccgctggcc tgtttcagcg ccagcgccgc cagttcgcgg
     7021 tcggcaatgc cgccttctgt catcacgcga tccaccgcga ggttaagctg ctgttcaatc
     7081 tgggcgacgc tcagttcggg caaatcggta tcgccccggc gtcggctctc ttgcagggcg
     7141 tgggcggcgt cgatcgcctt ttcgccccct ttcacggcaa cgtacatcag cacacctcca
     7201 catgagtggt tcgcgggata gccagcaggc gctcgccgca ggtcaggatt aggtcgatgc
     7261 cgagcgggaa cgggtgcggg cgctcggtga gttcgtgcag aatgcactct ggcagctgcg
     7321 gagcgatcat tcgttcttcg gcaatgcccg cgccggtgag acgcaacatg cgtccgccgc
     7381 tcaggctggc gacctgtaaa atcagcgtcg cgcccgcttc cggcgcaacg gcggtgtcgg
     7441 tggaaagggc gttgagctgt tcgctggaaa tcgcctcatc cgtcaccgcg aaggtcgcct
     7501 gttccggctg gctgaccagc ggcgcgttgg tatgaaaacg caggctctga ttgacgatat
     7561 cgttacttaa tggcgtggca agccacaccg gcgtgtcgtt atcggccagc gtcagcagca
     7621 cgctggtggt ggcgatattc agcggctgcc agccgcgttt gagctgatac agggcgacaa
     7681 tcacgcccgg ctcgctcatg gcctttaaca ggcgacgaaa actgtgctgg gcatcctgca
     7741 cgggaagcat aaaagcggtt tccagggtca tgcgttgtct ccgcgaacca tcgtaaagaa
     7801 gtcgacccgg ctggcgttca cttcggcctg gcgtgcggca atgcgtgcca tacggtcagc
     7861 gtccagcggg gcaataaggg tttctgataa gttttgaaag tggcgagatt gctgcatcag
     7921 cgcatcaatc agcgcgcagc gttcggcgtg ctgtttatca cgccccagca cccagctgta
     7981 gccgagggtg ccgtcagtca ggcgtacggc ggcacgggtc agcgtggcgt cgccggcaaa
     8041 aaagcgttcg ccggtaccgc ccattcgcgc ctgaatctgc accaggccag tttcggcagt
     8101 gcggatcact tcatagtcgg cggtgatgtt cagcgcgttc aggcgggctg ccagttcagc
     8161 cggttggctg tgcgccagta cggacatcca gtgctggcgg gtcgcggtat ctgcgtgcat
     8221 tcagtgctcc atagtgaatt caatcatgtc ggcgcgcgtc aggctgacgg agtactccgc
     8281 cgggctgctt tcaccgtcac ggtggttaag ggtgcgcacg cacagcagcg gcgacatatt
     8341 cgggatttca aggcgctggc actctttggc ctgggcgcgg cgggcgctga tccgcgtctg
     8401 gctgcggcgc agcgcaattc cggtttgctc gcgcagaaaa tcgtgcagcg aaccgctgtc
     8461 gaaacgttgc agtgtcggcc agagggtgag gtccgcaaag tagtggtcga ttaaacagag
     8521 cgcgacgccg ttgacgcgac gcagggtgcg caaatggata acgttctcac cctcggtaat
     8581 ccccagcgcg tcagcaacgt gaccggacgc ggggcgcaat accgacagca gtttttcgct
     8641 ggtgggatgg ctgccctggt ccagcaggtt ctggctaaaa cgcgcctggg cgttgagcgg
     8701 gtaatcgaac gggcgcatca gcaccagcac gccgatgccc tgacggcgct gtacccagcc
     8761 tttttccacc agttggtcga tggcgcggcg cagggtgtgg cggtttacct caaagcgcgc
     8821 cgccagttgc tgctcggcgg gaagatagtc gccgcagcga tagtgttgac gaagctcctg
     8881 ctcaagtttt gcggctatct cctgatagcg tgttgggtag ctggtcggat gtgtagacaa
     8941 gtgcatagat atcaatgcct cacttatcag ataaagtgct tacgcaaccg ttgagagagg
     9001 aaatccagca ggctgaccgt gacgatgata agcaccatca gggcgcaggt ttgttggaac
     9061 tggaaaccgc gaatcgcttc ccacagggtg acgccgatcc cgcctgcacc gaccatgccg
     9121 acgacggtcg ccgagcggac gttggattcg aagcgataga gggagtagga gatcagcagc
     9181 ggcattacct gtggcagcac gccgtaaagg atctcttcca gcttgttggc gccggtggcg
     9241 cgaatgcctt ccaccgggcc gggttcaatc gcttccaccg cttcggaaag cagtttcgag
     9301 agcacgccgg tggtgtgaat aaatagcgcc agcacgccag cgaacggtcc gagaccgacg
     9361 gcgaccacga acagcatggc gaagaccatt tcgttaatgg cgcggcaggc gtccatcagg
     9421 cggcgaacgg gctggtaaac ccaccacggc accaggtttt cggcgctcat caggccgaag
     9481 gggatggaga gaaccactgc cagcgcggtg ccccagacgg cgatttgcag cgtgacggcc
     9541 atttcggtga ggtaatcctg ccactggctg aaatcgggcg ggaagaagtc ggcggcgaag
     9601 gtcgccatgt tgccgccgtc tttgatcagc gtaagcgggg ccatttccgc gccctgccac
     9661 gagacgacca acacggcgag aacaatggcc cagctcagaa gcgagaacca gctgcgcttg
     9721 ggtggggcga tggtgatggt ttgcatgttt ggctccgttt aatgttttct ccctctccct
     9781 gtgggagagg gccggggtga gggcatcagc gtgcgcattt ccccctcacc ctaaccctct
     9841 ccctcaaggg agaggggacc gatcgagcac attactgcac cgctttactc accgaactca
     9901 tcgcgcttaa cgcgttgttc aggcggtcca ggtcatccag ctgcgcctga atctcggtgg
     9961 ttttcgccag cttgtcctgc tcattcagtc cgttattgct ttttacgccc tgaatctctt
    10021 taaacagtgc gagctggcga atcggcacca gttgcaggtc gctggaggcg cgaaacggtg
    10081 cccagcccag gcgttccagc accgctttct cttccggcgt ttttccgtag ttcataaaga
    10141 agtcgtagat cttgtctttg gtggtttcgg agagattctt gcgccagacg atcggatcgc
    10201 ctgggatcag cggcgacttc cagatcacct tcagttcttt cagcttctct ggcgcggagg
    10261 ttttcagctt gtcgaggttt tcggtgttgt tggtggcaac atccacctgc ttgttggcga
    10321 cggccagcgc gttggtttca tgcccggcgt tgacggtgcg cttaaagtcg ctggcggaga
    10381 tattgttttt ggcaaagacg tagtaaccgg ggacgaggaa gccagaggtg gagttaggat
    10441 cgccattgcc gaaggtgaga tctttccgct tcgccagcag atcgttcagg ttgttgatcg
    10501 gactatcttt gttgacgatc aacacgctcc agtaacctgg cgatccatcc gccgcgaccg
    10561 tctgggcgaa gacctggccg ttggcgcgat ccaccgcttc cattgccgac aggttgccgt
    10621 accaggcgat atccacttta ttgaagcgca ttccctggat aatgcctgcg tagtctgggg
    10681 caaagaaggc gttcaccttc acgcccagct tcttctccat atcctgtaag aatggcgtcc
    10741 attgcggttt caggttttgc tgtgattccg ttgaaataat gccgaaattc agcgcctttt
    10801 cctgctcttc ggcgtgtgcc gggcttaaca gggtgctgag gctgaacatg ctggtgaagg
    10861 ccagcgaggc aattatctta gcgttcattc gttttcctca atgatgggga tgtcaggcag
    10921 ctttcgcgtt ctcttcgatg cgattaatgc tgcggtagag atggtcaaaa cgttcgttat
    10981 caaactgttg gctgctgccg tcgtagaaaa cgtgcccctg gcgcagggcg acgatgcgtt
    11041 cgcagtagcg cagggcgtaa tccacctgat gcagcgtgac gaccacggtg atgccgtcgt
    11101 tctgattgat gtcgcgcagg gtgtccatca cgatgccggc ggattccggg tccagcgagg
    11161 cgatgggttc atcggccaga atcaccttcg cctgctgcat cagcgcgcgg gcaatcgcca
    11221 cacgctgctg ctgtccgccg gagagggtgg aaacgcgttg atgggcaaaa tgcaccatgc
    11281 caacgcgggt cagcgcctgt aacgcgcgtt gtttctgctc gcgggtaaac cagctaaaac
    11341 aggtgcgcca gaacggcgtg ctgccgagtg cgccaatcag cacgttctcc agtacgctca
    11401 ggcggttcac caggttgaat tgttggaaga tgtagccggt gttggcgcgg cttttgcgga
    11461 tatcgcgcgc cagacggcct tcgcgctgga ctgtgcggcc cagcagctcg atatggctgc
    11521 cggcggattt atcgccggta atcaaaccgc ttaagtgacg taaaagggtg gatttgccgg
    11581 aacccgacgg cccaagcaga gccaccattt caccgtgatg aatgttcaga tcaaccgcat
    11641 gcagcgcctg atgctgattg aaggttttgg cgagcttctc gacacggata atcgtttgca
    11701 tgatgcagcc tccctaaaaa agtggctcca tcgtggcgga ttattgtgac gatttggtta
    11761 attaaaagtg actaacagat gaagagttaa cgggaattcg atgacagtgc gggagggcgg
    11821 gcctcccggc gggttattgc gttggttgtt gtttgacgac attaatcatc cacggtacgc
    11881 caaatttatc ggtgactttg ccaaagccat gcgcctagaa agtttcctgc caggccattt
    11941 cgatttttcc gttagcggca agattgtcaa accagcgttt tccttcttcg acctgttgcg
    12001 aatcgagcac cagcgtaaag ccggagtagc tggcttttcc tgacggcatg gcatcgctca
    12061 tcatgatgtc gcttccggca atgcgcacat tggcgtgggc gatggcggta tcgggaaatt
    12121 gcattccgga agggcagttc tcggcgctgt cctgcgctga ttttggcatt tcgccgaagc
    12181 tgattttata gagcagttca gcgcccaacg tacgttgata ataagcaatt gcgtcggcac
    12241 agttaccggc aaaagagagg tagggactta acggcatgat ggtgacctca gttaagagaa
    12301 gccaattaag tgtagttcga aatttataca gatgagaggc tgcctgataa gacgcgcaag
    12361 cgtcgcatca ggcagtcggc atcgttgccg gatgcggcgc gagcgcctta tccggcttac
    12421 cgatcacaat acaatcagtt ctttttcaca aactcagatt tcagtttcat cggaccaaaa
    12481 ccgtcgattt tgcaatcgat gttatggtcg ccttcaacca ggcggatgtt tttcactttg
    12541 gtgccaattt tcagcatcga agagctacct ttcaccttca gatctttaat gatggtaacg
    12601 ctgtcgccgt cagccagcag attgccgtta gcatctttaa cgatcagctc gtcgctttcc
    12661 tgtgcaggtt ctgcgtcgtt ccattcgtag gcacattccg ggcagatgta catgccgtta
    12721 tcttcgtaag tgtattcgga gttgcatttt gggcagtgtg gtaatgacat gtggatttcc
    12781 tcaaaagtca gcagggcaaa agcgccaaaa aacggcagat tgccgaaaaa ggccgcaatt
    12841 atacacaaaa tccctgagtt tgtcgggggt actgctgatt ttagacgacg atcgttttca
    12901 tttgaaagga aacgcaa
//
LOCUS       DAZPLM010000114        12876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPLM010000114 DAZPLM010000000
VERSION     DAZPLM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.114"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..890
                     /gene="yhfZ"
                     /locus_tag="ACHVC7_002709"
     CDS             <1..890
                     /gene="yhfZ"
                     /locus_tag="ACHVC7_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR2116721.1"
                     /translation="KKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAAL
                     KTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGLK
                     AQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALELGPHTY
                     VGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVKG
                     DVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVDK
                     HALLAHQQRVVSGEQEPSY"
     gene            907..1269
                     /locus_tag="ACHVC7_002710"
     CDS             907..1269
                     /locus_tag="ACHVC7_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR2116722.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1353..2516
                     /locus_tag="ACHVC7_002711"
                     /pseudo
     CDS             1353..2516
                     /locus_tag="ACHVC7_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            2516..3742
                     /locus_tag="ACHVC7_002712"
                     /pseudo
     CDS             2516..3742
                     /locus_tag="ACHVC7_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            3739..4617
                     /locus_tag="ACHVC7_002713"
     CDS             3739..4617
                     /locus_tag="ACHVC7_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR2116723.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            4628..4981
                     /locus_tag="ACHVC7_002714"
     CDS             4628..4981
                     /locus_tag="ACHVC7_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR2116724.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            4993..6297
                     /locus_tag="ACHVC7_002715"
     CDS             4993..6297
                     /locus_tag="ACHVC7_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR2116725.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6309..7394
                     /locus_tag="ACHVC7_002716"
     CDS             6309..7394
                     /locus_tag="ACHVC7_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR2116726.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(7546..8277)
                     /gene="frlR"
                     /locus_tag="ACHVC7_002717"
                     /gene_synonym="yhfR"
     CDS             complement(7546..8277)
                     /gene="frlR"
                     /locus_tag="ACHVC7_002717"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR2116727.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(8377..9162)
                     /gene="frlD"
                     /locus_tag="ACHVC7_002718"
     CDS             complement(8377..9162)
                     /gene="frlD"
                     /locus_tag="ACHVC7_002718"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR2116728.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9159..9990)
                     /gene="frlC"
                     /locus_tag="ACHVC7_002719"
                     /pseudo
     CDS             complement(9159..9990)
                     /gene="frlC"
                     /locus_tag="ACHVC7_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(10040..11062)
                     /gene="frlB"
                     /locus_tag="ACHVC7_002720"
     CDS             complement(10040..11062)
                     /gene="frlB"
                     /locus_tag="ACHVC7_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR2116729.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11083..12420)
                     /gene="frlA"
                     /locus_tag="ACHVC7_002721"
     CDS             complement(11083..12420)
                     /gene="frlA"
                     /locus_tag="ACHVC7_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR2116730.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(12715..>12876)
                     /locus_tag="ACHVC7_002722"
     CDS             complement(12715..>12876)
                     /locus_tag="ACHVC7_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR2116731.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         2969 a         3228 c         3643 g         3036 t
ORIGIN      
        1 tcaaaaaaga aggcgtcgtc atcacgacgc tggcccgtta tttgttgggt gaaaaatgcg
       61 gtaatcgact gaaaaccata gatgagctgg caactgaatg ccgttcatcg gttggcctga
      121 cgcaggccgc gttgaaaacg ttggaatcaa gcggagcgat acggattgaa cgccgtgggc
      181 gcaatggcag ttatctggtc gagatggata acaaagcatt gctgagtcat gtggatatca
      241 acaacgtggt atgtgcgatg cctttgccct atacccgctt gtacgaaggt ttagcgagcg
      301 ggctgaaagc tcagtttgat ggcattcctt tttactatgc gcatatgcgc ggcgcggata
      361 ttcgcgtgga gtgtttactt aacggcgtgt atgacatggc ggtggtttcc cgactggcgg
      421 cggaaagtta tctcacgcaa aaagggttat gcctcgcgct ggagttgggg ccgcacacct
      481 acgttggcga gcaccagttg atttgccgta aaggcgagtc cgcaaacgtg aagcgcgtgg
      541 ggctggataa ccgttcggcg gatcagaaaa tcatgaccga tgtctttttt ggcgatagtg
      601 atgtggaacg tgtcgatctc tcttatcacg agagtttaca acgcattgtt aaaggcgatg
      661 tcgatgcggt tatctggaac gtggtggcgg aaaacgaact gaccatgctg ggattagagg
      721 cgacgccgct cacagacgat ccgcgatttt tacaggccac cgaagccgtg gtcctgacgc
      781 gagtcgatga ttacccaatg caacaactgc tgcgtgccgt tgtagataaa cacgccctgc
      841 tcgcccatca acaacgggta gtgagtgggg aacaggaacc gagttattaa acgaaaggca
      901 tctgatatgg aaaccagact caacctgctt tgcgaggcag gcgttattga taaggacatc
      961 tgcaaaggca tgatgcaggt cgtcaacgta ctggaaacag agtgccatct gccggtgcgc
     1021 agtgagcaag gaacgatggc gatgacacat atggcgagtg cactgatgcg cagtcgccgt
     1081 ggtgaagaaa tagagccgct ggataacgaa ttgctggcag agctggcgca atccagccac
     1141 tggcaagccg ttgtgcaatt gcatcaggtg ttgttgaagg aattcgcact ggaagttaac
     1201 ccgtgtgaag aaggctattt gctggccaac ctttatggat tatggatggc tgctaacgaa
     1261 gaggtttgaa gctcccgaca aatccacgtc gcacaaccta aatattcaaa aaaatgaata
     1321 tttaattcaa ggaacagaaa cgaggcaaca agatgtttgt agaagcattg aaacgccaga
     1381 acccggcgct gatttccgcc gcactaagtt tgtggcagca gggcaagatc gccccggaca
     1441 gctgggtgat cgacgtggat caggtactgg aaaacggtaa gcgactgatt gagacggcgc
     1501 ggctttacgg cattgaactg tatctgatga ccaagcagtt tggccgtaac ccatggctgg
     1561 cggaaaaatt gctggcatta ggttacagcg gcattgtggc ggtggattac aaagaggcgc
     1621 gagtcatgcg ccgcgctggt ttgtcggtgg cacatcaggg gcatctggta caaatccctt
     1681 gtcatcaggt tgctgacgcc gttgaacagg gcaccgacgt catcaccgtg tttactctcg
     1741 acaaagcgcg ggaagtttct gcggcagcgg tgaaggccgg acgtgtgcag tttgtgctgc
     1801 tcaaagttta tagcgacgat gattttcttt atccggggca ggagagcggt tttgtacagc
     1861 attcgctgca cgaggtggtt gctgaaatta agaaactgcc ggggctgcat ttagccggac
     1921 ttacccattt cccttgcctg ctttgggatg aggctgccgg aaaagttttg ccgacaccga
     1981 atcttcacac gctgatacag gcacgggatc aactggcgaa atctggtatt gcacttgagc
     2041 aactgaacgc gccttcagcg accagctgca cttcgctgcc attactggcg taatacggtg
     2101 tgactcatgc cgaacccggt catgcgctga cgggcactat tccggcaaac cagcagggcg
     2161 atcagtctga acgtatcgcg atgctctggt tgagtgaaat ctcccatcat ttccgtggcg
     2221 acagctactg ctacggcggc ggttactacc gtcgtggtca tgcgcaacat gcgttggtgt
     2281 ttacgccaga aaatcaaaag attactgaaa ccaatctcaa aactgtggat gacagcagta
     2341 tcgactacac cctgtcgctg gcaggcgagt ttccggtaag cagtgcagtg gtgctctgtt
     2401 tccgcacgca gatttttgtc acccgtagcg atgtggtgct ggtgtccggt attcaccgtg
     2461 gcgaaccgaa aatagttggt cgttatgaca gtcttggaaa ctctctgggg gcgtaatggc
     2521 gcgatttgtg gtgttagtga ttgatagctt tggcgtaggg gcaatgaaag atgtcacgct
     2581 ggtgcgtccg caagatgcgg gagcgaatac atgtggtcac atcctgagcc agttgccgca
     2641 tttgcagcta ccaacgctgg agaagctggg gctaatcaac gcattgggtt atgcgccagg
     2701 cgatatgcag ccgtcagatt ccgcaacctg gggcgtggca gagctgcaac atgaaggtgg
     2761 cgataccttt atggggcatc aggaaatttt aggcacgcgc ccgttaccgc cgctgcggat
     2821 gccttttcgc gatgtgattg accgtgttga gcaggcatta gtatccgccg gttggcaggt
     2881 ggagcgccgt ggcgatgaac tgtaatttct gtgggtcaat caggcggttg cgattggcga
     2941 taatctcgag gcggatttag gccaagtcta taacattacc gccaatctct ctgtgatctc
     3001 ttttgacgac gcaatcaaaa ttggtcgtat cgtgcgtgag caggtacagg tcggtcgggt
     3061 cattacattt ggtggcctgt taaccgacag tcaacgcatt ctcgatgccg cagaaagcaa
     3121 agaagggcgc tttattggta tcaatgcgcc gcgttctggc gcttatgaca acggcttcca
     3181 ggtcgtgcat atgggctacg gtgtcgataa aaaagtgcag gtgccgcaaa aactgtatga
     3241 ggcgggcgtg ccaaccgtgc tggtgggtaa ggtggcagat atcgtcagca atccttatgg
     3301 cgtgagctgg caaaacctgg tggatagcca gcggattatg gatatcaccc tcgacgaatt
     3361 taacacccat ccgacggcgt ttatttgcac caatatccag gaaaccgacc tcgctggtca
     3421 tgcagaagac gtcgcacgtt atgccgaacg tttgcaggtc gttgaccgta accttgcccg
     3481 gcttgttgat gcgatgcagc cagatgattg cctggtcgtg atggcggatc acggcaacga
     3541 tccgaccatt ggtcacagcc accatacccg cgaagtggtg ccgctgctgg tttatcagca
     3601 aggcctggct gctacgcagc tcggtgtgcg caccacgctt tctgatgtgg gagctaccgt
     3661 gtgtgaattt ttccgtgcgc caccgccaca aaatggtcgc tcttttcttt cctccctccg
     3721 gtttgcagga gacaccctat gagttttgat ccgacgggtt acaccctggc ccatgagcat
     3781 ctgcatattg atctctccgg ctttaaaaac aacgtggact gccgccttga tcagtatgcg
     3841 ttcatttgcc aggagatgaa cgacctgatg acccggggtg tacgtaatgt gattgagatg
     3901 accaaccgtt acatggggcg caatgcgcaa tttatgctcg gtgtaatgcg cgaaaccggg
     3961 atcaacgtgg tggcctgtac tggttattac caggatgcgt ttttcccgga acatgtggtg
     4021 acccgcagcg tgcaggaact ggcgcaggag atggtcgatg aaattgaaca gggtatcgat
     4081 ggcacggagc tgaaagccgg gatcatcgcg gagatcggct ccagcgaagg aaagattacg
     4141 ccgctggaag agaaggtatt tattgccgct gcgctggcgc ataaccagac cggacgcccg
     4201 atctccacgc atacgtcgtt cagcaccatg gggctggaac aactggctct gttgcaagcc
     4261 cacggagttg atctttcgcg cgtcaccgtt ggtcactgcg atctgaaaga caacctcgac
     4321 aacattttga agatgatcga tctcggcgcg tacgtgcagt tcgacaccat tggtaagaac
     4381 agttactacc cggacgaaaa gcgcattgcg atgctccacg cgctacgtga ccgtgggttg
     4441 ctgaaccgcg tcatgctgtc gatggatatt acgcgccgct cccatttaaa agccaatggt
     4501 ggttatggct atgactattt attgaccacc tttattccgc aattgcgcca gtcaggattc
     4561 agtcaggccg atgtggatgt gatgttacgt gaaactccct ctcaattttt ccaataagga
     4621 cagactcatg aaaaagattg gcgttgcagg cttacagcgt gagcagatta aaaaaaccat
     4681 tgaagcgacg gctcctggca gttttgaagt tttcattcac aacgacatgg aagcggcaat
     4741 gaaagtgaaa tccgggcaac tggattacta catcggtgcg tgtaataccg gtgcgggcgc
     4801 ggcattgtcg attgccatcg cggtgattgg ctataacaaa agttgcacca ttgccaaacc
     4861 aggcattaaa gcgaaagacg agcatatcgc caaaatgatc gctgaaggaa aagtggcgtt
     4921 tggcctttcc gttgagcacg tcgaacacgc gattccgatg ctgattaacc atctgaaata
     4981 aaaggcacga ctatggatct gtatattcag attatcgtgg tggcgtgcct gacgggtatg
     5041 acatcgcttc tggcgcatcg ctcggcggct gtttttcatg acggtatccg cccgatcctg
     5101 ccgcaactga ttgaaggcta tatgaaccgt cgcgaggcgg ggagtatcgc ttttggtctg
     5161 agcattggtt ttgtggcctc ggtggggatc tcttttaccc tgaaaaccgg gctgctcaac
     5221 gcatggttac tctttcttcc taccgatatc ctcggcgtac tggcgataaa cagcctgatg
     5281 gcgtttggtc ttggcgctat ctggggcgtg ttgatcctta cttgcctgtt gccagtaaac
     5341 cagctgctga ccgcgctgcc ggtggatgta ttaggtagcc tcggggaatt aagctcgccg
     5401 gtggtttctg cttttgcact gttcccgctg gtggcgattt tctaccagtt tggctggaag
     5461 caaagtctgg tcgccgccgt ggttgttctg atgacccgtg tggtagtcgt gcgctatttc
     5521 ccacatctta accctgaatc catcgaaatc tttattggca tggtgatgct gctggggatc
     5581 gcgataactc acgacctgcg tcatcgtgat gaaaatgata ttgatgccag cgggctttcg
     5641 gtgtttgaag aacgcacgtc gcggattatc aaaaacttac cctatatcgc catcgtggga
     5701 gcattgattg ccgccgttgc cagcatgaag attttcgccg gcagtgaagt gtcgatcttc
     5761 actctggaga aagcgtactc cgcaggcgta acgccggaac aatcgcaaac gctgatcaac
     5821 caggcggcac tggcagaatt tatgcgcggg ctgggttttg taccgttgat tgccaccacc
     5881 gccttagcaa cgggtgtgta tgcagttgcg ggctttacct ttgtttatgc ggtgggctat
     5941 ctctcgccga atccgatagt tgcagcggta ttaggcgcag tggttatttc ggcggaagtc
     6001 ttgctgcttc gttcgatcgg caaatggctg ggacgctacc cgtcggtgcg taatgcgtcg
     6061 gataacatcc gtaacgccat gaatatgctg atggaagtgg cgctgctggt cggttcgatt
     6121 ttcgcagcaa ttaagatggc gggttatacc ggattctcta tcgcggttgc catttacttc
     6181 ctcaacgaat ccctgggccg tccggtacag aaaatggcgg caccggtcgt ggcagtaatg
     6241 atcaccggta ttctgctgaa tgttctttac tggcttggcc tgttcgttcc ggcttaagga
     6301 aggcacctat gaagacgttt cctctgcaaa gtctgacgat tattgaggcg cagcaaaagc
     6361 agtttgcgct ggtggatagc atttgtcgcc atttccccgg cagcgagttt cttactggcg
     6421 gtgatttagg cttaacgcca ggactgaatc aaccgcgcgt tacccagcgt gtggaacagg
     6481 tgctggctga tgcatttcac gcacaggctg cggcgctggt gcagggtgcg ggtactggcg
     6541 cgattcgtgc ggcgttggcg gctttgctca aaccggggca gcgtcttctg gtacatgatg
     6601 cgcctgttta cccgacgaca caggtcatta ttgagcagat ggggctgacg cttattactg
     6661 ctgatttcaa tgacctgtcg gcactgaagc aggtcgtcga cgagcaacta ccggatgcgg
     6721 cgctggtgca gcatacgcgc cagcagccgc aggacggcta cattctggca gatgtgctgg
     6781 caacattgcg ctcggctggc gttcctgcat taaccgatga caactatgcg gtgatgaaag
     6841 tggcgcgaat tggctgcgaa tgcggggcaa atgtctcgac tttttcctgc ttcaagctgt
     6901 ttgggccgga gggtgttggc gcggtggtag gcgatgccga tgttattagt cggattcgct
     6961 ccacgctcta ttccggcggc agccagattc agggcgctca ggcgctggaa gtcttgtgtg
     7021 ggctggtgtt tgcgccagtg atgcacgcgg tgcaggcggg ggtatctgaa cggttgctgg
     7081 ctttgcttaa cggtggtgcg gtgccggaag tgaaaagcgc ggtgattgct aatgcgcagt
     7141 cgaaggtgtt gattgtcgag tttcatcagc cgattgccgc cagagtgctg gaagaggcgc
     7201 aaaagcgcgg tgccttgcct tacccggtgg gtgcagagtc gaaatatgaa atcccgccgc
     7261 tcttttatcg cctttccgga acgtttcgcc aggcgaatcc acaatcagaa cattgtgcga
     7321 ttcgcattaa cccgaatcgc aacggtgaag agacggtgct gcggattttg cgtgagagta
     7381 ttgccagtat ttaatgctgg atagcaccgt caagctaaat tccgtactga acggtcccct
     7441 cgcccctttg gggagagggt tagggtgagg ggaacgtacg ggatgtaaaa ttcaacgcgc
     7501 cagcatcgcc ggatgcgatg ctggcgcatc ttatccggcc tacggttaat gtcttttatt
     7561 atcaatcgtt aaggtgatgc gattggcgcg gcagaagagt tcggagacgt gcaccggttt
     7621 gtcattctga tcaaaggcga ttttgctgat gcgaaacaac ggttcgccca gttcgcattt
     7681 cagccatttt gcctgcgggc gggtggcggc aaagatatcg atggtctttt tgtcgctgac
     7741 cactcgcgtg tcaaaacgct cctgaaataa ctgataggtg gagcttcctt cgacgtaaat
     7801 ctcatcaaag tcaggataac gcgacagcgg gatccatgaa ctatcaataa acagcggctc
     7861 tttatcgaga tacatcaccc ggcagagatg gaacacttcg ctgtttccgg ggatgttcag
     7921 cttttcgcaa aacggcgcgg cgctgacccg ttcctgttcg atcactttct ctttcgtcgc
     7981 cttgccttgt gagacgccaa aatcggtaaa accactgaca gtaagcaggg cgttttcaac
     8041 tttctggctt tgtacaaagg tgcctttgcc ctgccagcgg atcagtacgc cgtctgccac
     8101 taagtcgctg atagctttgc gaatagtaat gcggctgacg ttatattgcg tacaaagctc
     8161 gttttcggta gggatctgtt gcccggcctg gtaaaccccc tgcgcgatat catccagcag
     8221 ccgctggcgg acggtagcgt agaggagttg atgagagtag cggtccgtag ctgacataat
     8281 cgaccctgaa ataaagaatg aattacacgg attatagggt cgttttccgg caagtaacag
     8341 cacgttggcg cggagatgct cacgccaacg ttatacctac caggcaccgt gatactgaat
     8401 ggttttcgcc gcgcacgccg ttccctgcgc catcgcctgc ggtaatgtca tccccgcaga
     8461 ccagccgcaa aggaatccgg caatgaacga atctccggca cccatggtgt cgataaccgt
     8521 caccggttca ggagcctgac gccagaactg cgcgccatcc caggcaatgc tgccgttttc
     8581 acccagcgtg acaatcactg ttcctgcgcc acgggcaaca atcgctttca tcttcagacg
     8641 cagcgcttcg tcttcttgcg gtgcggaggc aaaggcaaaa tcgagatgcg gcaccagtgt
     8701 ctgccagagc gggctgtccc acttgtcgga gaagtcgaaa gcggtaagtt tgcccgcagc
     8761 gtgcagctgt gggaatgcgt cttccgcatg tccccagatt gccgcgtgca caatgtcata
     8821 ctgcgccagc caggcgtaat cctcttcact cagggcaaag tcggccatca cgccttcggt
     8881 gtagtcgcca aaaacgcgat cattgtcgtg cagttccacc tgagtttgtg cggtaacgcc
     8941 gtgtttcgta tggacatggc tgatatcgac gcccatgcgg gcgagatcct gcttcagctt
     9001 tgtgccgtag tcatcgtcac ccaccaaggt aatgcatccc ggctgtatgc cgtagcgagt
     9061 gcagtacacc gccacattga ccgcattacc gccagaaaac gctttattca gttgcgggta
     9121 gatatcgacg cagttatcgc cgattgtcgc cagggttttc atctctcatc ctccggcagc
     9181 agcgcgcgaa agcgttccag cgcctggcgg gcatagagtc tgggctcgtt catatacatc
     9241 gtcaccagct ccaccgtaca gtaaccctca tagccccgct caataatatc gcgcatcagt
     9301 tcccgcagcg gcattttgcc ttctccagga atgtaatgcg tgtcgctggc cccgtcgctg
     9361 tcgacaatat gcagatgacg ataatttatc gcccagtttg tcgaaataac tcatcaccgg
     9421 ttccgcctgg acatacggcg cgcaaatgtc gaccatgctg aacaggcgcg gcgaaggcac
     9481 cagcgccagc gcatgaagca catcattagc attacacacg acgttcgatt catacggcgt
     9541 taacggttcg aggattagat ccatgccgat gttttccgcg tactcacaca gttcgcttag
     9601 gttctctgcc agccgtcccc agataacatt aggtggcgtg agatagcctg cgtgggccgc
     9661 ggaaatcagc gtataacccg cgttcatctc ttttgccata tccatcgcca gcttgatcat
     9721 gtcgaggctt tcgcgacgca tatgttcatc gcccagcatc atgttatacg gatagccgtt
     9781 ggtttctggc gtatagccga taatcggcat ctgatacgtc tgcgccagcg ccttgatttg
     9841 tttgatgccg cccgctttta agtccggcgc gaacgcgtgc gggcgaccgc cccaaatttc
     9901 gatgccgtcg taacccagct cgcttgcatc acgaaatgcg tgttcaatag gcagccgctg
     9961 gtggccgcag gtaaacatac ctgttttcat cgggtaactc cttgcgtaaa tccggcccgt
    10021 tgccgggccg ggagagggat taatattcca ccagaccacc gtaatagcgg cgttcatccg
    10081 ggttgtgatc tttgtaaata gacaggtagt agcagagcca ctccattggc acgaacatca
    10141 ggaacggtgc cagccacggg tgcagccctt gcgaaatttc ggcgtaatcg atgacgatca
    10201 cgttgtcagt acgctgttta acaaagttaa tggcgcgttc ggtggtgtgg cgactttcat
    10261 cattgccgag caggaacagg aacggaacgc ccggttcgac aatctccagc gggccatggc
    10321 ggaactctcc gctctcaatc acgcagccgt gcgtccaggt aaattccatc agcgttacaa
    10381 tgccttcttt gtaacccagc ggacgcagcg gacccgcagc aacggtataa atcatcggcc
    10441 actggctggc cagttcacca agctggcggc ctttttcttc ccaggtgcgt accagatgac
    10501 cgagcgcatt cggcaacggc ttgagatcgt ttttgatctt gccgatttcc gcgttcggcg
    10561 cgaggcgggt gatcatctcc agcaccacgc tgtagcagag cagcaggtga atttcccaga
    10621 tacagtcggc ctgataatca atgctaaatt ccgccgccga ggtaatcggg ctgtccgcgc
    10681 gtttggtgaa cgccgcagtg agtgcgccgc aggcccggcc cagctccagc gctttgatta
    10741 cctcttccgt tttaccgtag tcagaaacgc caatcaccgc gcaacgatcg tcgaggcgat
    10801 acggggtgtt atcgcagaac tcccagccgg aaatggcgta gacctgaaga tcggaaaagc
    10861 gatccgccag atgtttcgcc gtttgcgccg cgttgagtgg cgagccgcag gcaacgaaat
    10921 aaatacgatc aatgtcgcgc ttcaccatct cttccacgat ggcgtgcacc agcggaacgt
    10981 catggctgag aactttttcc acttcctgaa ccatattttc ggtgaccaga aagtccacgg
    11041 tgcttttatc aatatccaac attttatttt ctccagataa cattacgaca aagcgttgag
    11101 ctggcggcta cgcttcgccc agaaggcgta agcaggcaga ccagtagcaa taacgatgac
    11161 ggcgcagata aggccgggaa tcggtgccca gacaaaggtt gaggcgacca gaatgaggct
    11221 tgacgcaatg gcgagggtgg tcatcagccc gaaagccgga gtacgccaca gcggtttgta
    11281 atcgtcgcgt ttacgacacc agatgatgga gccgaaggtg agggtatttt tgaaacacat
    11341 caccagggtg aaataaccca gcaggctggt gagatcggaa acgaagatga agaagatccc
    11401 cagcgcccct tgcaggatga tggagacatc cggcgtgttg tatttcggat gcacatggcc
    11461 gaagcattta aagaacaggt tgtctttcgc catcgcgtat tccagccgcg gctggtacat
    11521 cacgcagctg gaaagcgaac caagaatgac gatcatcgcc gtgatggcaa caaagatccc
    11581 agcggtgctg ccgagtgcgg ggatccaggt cagggcgtcg gaaatcggcg tttcagaatt
    11641 ggcgagtttg tcgaagggca tcaggccgga aatcaccagc gccagcaggg tgtagagcac
    11701 cagaaccagc agacaggaac caatcagcgc tcgtggcatg gtttttccgg ggtttttaat
    11761 ttcgccggtc atataacaga tagaggccat gccggtatac gaccaactgg tggcagagat
    11821 ccccgccagc agcgccataa agctgcccgt tgcgccaatc gcagtggtgg taggggcggc
    11881 aaaattctcc gctttaaacc agaagatccc aaggccaatg acgatagtga acgggataat
    11941 tttggcgatg gtaattagcg tctgaaacgc tgcgccgcct tcaacggagc gcaggtgtag
    12001 caacataaag gcgataatta atccggcggc gataaattta ccgagcaacg gatcgatagg
    12061 cgttaaaaag ccaagattgc tgacaatcgc cagcgccata atcgacaatg acggtgcatc
    12121 gttggcccag aagctggccc agccggagag gaaagccagc ggtcggcttc cggcattttt
    12181 cagataaaca taatctgcgc cattttccgg ataagcggtg gatagttccg catagacgca
    12241 catttgcggg atcacaatta acccgccaat gacaaacgcg agcaccgtaa gccacggcgt
    12301 gcccgctgct tttgccactt cacccacaga tacaaaaata ccggagccga cggttgtccc
    12361 gacggcgatt gcaagaacgg cccaaaatcc gagcttgcgt tggagttcct ggcttcccat
    12421 agcgatacct ttttcttgtc caatacgttg taggggagag tttgcagtca tttaaaaggt
    12481 aacatgacaa tgcatgatga atataacaac atatgatgtt atgtaatgtg gaggaagatc
    12541 acaaaatttc gcacaggatc gcgctgtggc taatggatgt agttatcaaa ttgaatttaa
    12601 agtgaaaata tttttacggg cgggggcaag aaggacatag aaacaaatac gccctcggaa
    12661 aatccagagg gcgtcgggca attaaaccgg tgttagccga tttctgtcag agacttactg
    12721 tgcagtagga ccgcaaccgc caatgatttt agaaatagaa attgccgggt gcagcaggta
    12781 gtcgtaagag cagttcttat cacggttttc aatatgacca gtacatgcag ttaacgtagc
    12841 cagtactgca cctaccagtg caactttaat aaattt
//
LOCUS       DAZPLM010000115        12710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPLM010000115 DAZPLM010000000
VERSION     DAZPLM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.115"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..1889)
                     /gene="espL1"
                     /locus_tag="ACHVC7_002723"
                     /pseudo
     CDS             complement(1..1889)
                     /gene="espL1"
                     /locus_tag="ACHVC7_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2414..4342
                     /gene="thrS"
                     /locus_tag="ACHVC7_002724"
     CDS             2414..4342
                     /gene="thrS"
                     /locus_tag="ACHVC7_002724"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR2116732.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4346..4888
                     /gene="infC"
                     /locus_tag="ACHVC7_002725"
     CDS             4346..4888
                     /gene="infC"
                     /locus_tag="ACHVC7_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR2116733.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4985..5182
                     /gene="rpmI"
                     /locus_tag="ACHVC7_002726"
     CDS             4985..5182
                     /gene="rpmI"
                     /locus_tag="ACHVC7_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR2116734.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5235..5591
                     /gene="rplT"
                     /locus_tag="ACHVC7_002727"
     CDS             5235..5591
                     /gene="rplT"
                     /locus_tag="ACHVC7_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR2116735.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5708..5832
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5714..5758
                     /gene="pheM"
                     /locus_tag="ACHVC7_002728"
     CDS             5714..5758
                     /gene="pheM"
                     /locus_tag="ACHVC7_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR2116736.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6041..7024
                     /gene="pheS"
                     /locus_tag="ACHVC7_002729"
     CDS             6041..7024
                     /gene="pheS"
                     /locus_tag="ACHVC7_002729"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR2116737.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7039..9426
                     /gene="pheT"
                     /locus_tag="ACHVC7_002730"
     CDS             7039..9426
                     /gene="pheT"
                     /locus_tag="ACHVC7_002730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR2116738.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9431..9730
                     /gene="ihfA"
                     /locus_tag="ACHVC7_002731"
     CDS             9431..9730
                     /gene="ihfA"
                     /locus_tag="ACHVC7_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR2116739.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9831..10811
                     /gene="btuC"
                     /locus_tag="ACHVC7_002732"
     CDS             9831..10811
                     /gene="btuC"
                     /locus_tag="ACHVC7_002732"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR2116740.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10874..11425
                     /gene="btuE"
                     /locus_tag="ACHVC7_002733"
     CDS             10874..11425
                     /gene="btuE"
                     /locus_tag="ACHVC7_002733"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR2116741.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11425..12174
                     /gene="btuD"
                     /locus_tag="ACHVC7_002734"
     CDS             11425..12174
                     /gene="btuD"
                     /locus_tag="ACHVC7_002734"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR2116742.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12251..>12710
                     /locus_tag="ACHVC7_002735"
     CDS             12251..>12710
                     /locus_tag="ACHVC7_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR2116743.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         3258 a         2891 c         3344 g         3217 t
ORIGIN      
        1 cgtctttatt tcatcagcac taaaactcag gctttggctg atgacattta tagcagcata
       61 atatgtttct acagttttat aatgcttatg atgaatggct atatgaacag ctgacatgtt
      121 gtcatgattt ttagcggcca atagtgtaaa taactgatgt tgactaaaag aatgtttttt
      181 gcaaaagcac ccagcgtcga tatataagat aacacgacgt cttcattacc cttgctcatg
      241 gcgatgtata atgcaggggt tccctgtgct gtagctcctt ttaataaatc catgatgtta
      301 gctttgctca acttgtattt aaaggcgatg ccattaattt tagagaggaa ccgtgtgaca
      361 cacaaagggt gattattttc cattgcggcg tataatcctg gctcaccatc atgtattttc
      421 atggctaaaa tactcatcac catttttttg tttaaaagtt cttgtgctat tggagtggtt
      481 aatgcattca gaaagatatc gattatttca tcgaatccat acaacatgga gatatagagt
      541 ccaggtgttt cgttagcact tttagtttgc agtaatttaa ccagattatc ttcatgaatg
      601 atatgattgt ataccagtga ctgaatttct tgcataaata tttttacgtt gtcagtttgt
      661 ccgttcatca tagccagaaa taaagcaggt gtaccaacgc tattctgcgc agttattatt
      721 tcaatgattt ctttttcagg gagtatgggc aactgtttga atagtgtaat aagctattga
      781 gtctggcctg tggccattat taacaacatt gccgatggat gtataggggc aaatggcatt
      841 cgtgtcgtga tacccgctaa aatagcaact ggaactccct caggtaatag aggaagaata
      901 gcaattgcac agttgcgaac atacctatca gcccaggtct gagcatcggg agctgaactg
      961 atgaacttat caagcgagag aaagccccgt gctgccctgt gggtgcctct gacggcaacg
     1021 ttggtatcgt tcgggtcgta aacagacaca acataattta attgtccata cactgactgc
     1081 tttattcgta gccttactga caaagtatga ttgtctatat ataaaatcgc ggacatatga
     1141 ctgacattgt ttgccatcat tcgacaaaag aaaatatcta aagcgaagcc aaaatcctta
     1201 ctcgcgataa cgcgactccc tgcaggagga tggcgaagta atgctctgaa ctccgcgtcc
     1261 cggactaagg ggacatgctg ctgtatggat tcaacggagg agtagtgttt aatcaggttg
     1321 actttgcctt ttccatttct caatgaatca agggcgtatt gcaatgacag atggcgacaa
     1381 aaaatagcgt cagaaggaga ttgcgaaaaa aatgcactac cattgagatt tactgttggt
     1441 tgtatagttt tttctctatg gattttttta gacagtttca tacaatggtt acgatcttcg
     1501 gcagttaact gttcctgttc aatataatcc agtatgcttc gcaaatggtt agccttaata
     1561 ggcgtggttt ccagaaataa gggaatgatt tcttttttat agtgtaatgt aatgagtata
     1621 ttttctgcat ggatgggtga agtggcaaag ttaatgataa aaagggcagt ggaataacaa
     1681 ttttgtgaaa tttttcgaca gtttgcggta aattcctgag taacacagta tgacagcatc
     1741 gaacttaagt cagaggtatc aagtagggtg atgagttggc gcgcaaattg attaataggc
     1801 tctgatgaag gcgttggtat gtcgctatcg atgtgcaatc tatgaggaga cttataatgg
     1861 gttctgataa tgatatcgtt ttgactcata atctattcgc cttatatatg tctggaggtt
     1921 ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgctataag
     1981 aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctca tgagcgttta
     2041 aaatactacc gccaaatcac gcaacgaact ttgctggatt cagtttatgc tgctgtaaat
     2101 ccgctcgcag taaacctttc agacgcaccg tgatgttatc agtggttctt taagcgtttt
     2161 gctggtgtac tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa
     2221 tctccctttt tttagtgtta acatcgttca accgggtgga aggtgagagg ttaatgtctt
     2281 tcatttgtgt acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg
     2341 aactgttaat aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa
     2401 ttaaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca
     2461 cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat
     2521 cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca
     2581 actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc
     2641 gcacctgtta gggcatgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc
     2701 ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga
     2761 tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa
     2821 gaagaaagtc agctggcacg aagcgcgtga aactttcgcc aaccgtgggg agagctacaa
     2881 agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga
     2941 agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt
     3001 caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca
     3061 acgtatttac ggtacggcgt gggcagacaa aaaagcactt aacgcttacc tgcagcgcct
     3121 ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat
     3181 gcaggaagaa gcgccgggta tggtattctg gcacaacgac ggctggacca tcttccgtga
     3241 actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc
     3301 gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc
     3361 aatgttcacc acatcttctg agaaccgtga atactgcatt aagccgatga actgcccggg
     3421 tcacgtacaa attttcaacc aggggctgaa gtcttatcgc gatctgccgc tgcgtatggc
     3481 cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgcgt
     3541 gcgtggattt acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga
     3601 agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat
     3661 cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg
     3721 tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac atcccgtttg aatatcaact
     3781 gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg
     3841 tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc
     3901 ctatgtgggc gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattctggg
     3961 gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg
     4021 gcttgcgcca gttcaggttg ttatcatgaa tattaccgat tcacagtctg aatacgttaa
     4081 cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga
     4141 gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata tgctggtctg
     4201 tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct
     4261 gggaagcatg gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag
     4321 tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc
     4381 taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga
     4441 gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga
     4501 cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa
     4561 attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca
     4621 ggttaaggaa attaaattcc gtcctggtac agatgaaggc gactatcagg taaaactccg
     4681 cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg
     4741 tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt
     4801 gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat
     4861 ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg tggagccttc
     4921 gggcttcaca ggctttgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat
     4981 taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa
     5041 aggtggtttt aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa
     5101 acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat
     5161 cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac tttttaatta gaatatagat
     5221 acaggagagc acatatggct cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga
     5281 aaattttgaa acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct
     5341 tccaggctgt tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc
     5401 agttccgtca actgtggatt gcgcgtatca acgcagcagc acgtcagaac ggtatttctt
     5461 acagcaaatt catcaatggc ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg
     5521 ctgatatcgc agtattcgac aaagtagcgt tcaccgctct ggttgaaaaa gcgaaagcag
     5581 ctctggcata agccagttga aagagggagc tagtctccct cttttcgttt caacgccatc
     5641 aaaacattga cttttatcgc cgtagccttt tcaataaagg tcttttgaag agtaccaaaa
     5701 ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt ttttacttta gcacctgaat
     5761 ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga
     5821 ggcttttttt gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc gtaattgtcg
     5881 cagccagtct ggacacggcc agcgcggaga aaccggagcg tatttaagta cgtgagaatt
     5941 tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt
     6001 ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact
     6061 ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt
     6121 gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga
     6181 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt
     6241 tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gcagcgctga atgcgcgtct
     6301 ggcggcggaa acgattgatg tctccctgct aggtcgtcgc attgaaaacg gcggtctgca
     6361 tccggttacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt
     6421 ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg
     6481 tcaccacccg gcgcgcgctg accacgacac tttctggttt gacactaccc gcctgctgcg
     6541 tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat
     6601 catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca
     6661 tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga aaggcactct
     6721 gcacgatttc ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta
     6781 cttcccgttc accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct
     6841 ggaagtgctg ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc
     6901 ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta
     6961 cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa
     7021 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg
     7081 gcgattgata gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt
     7141 gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg
     7201 cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg
     7261 ctggacatcg tctgtggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc
     7321 attggtgctg ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg
     7381 tctgaaggga tgctgtgctc cttctctgaa ctgggaattt ctgacgatca cagcggcatt
     7441 atcgaactgc ctgcagatgc gccgattggc actgacatcc gcgaatacct gaaactcgat
     7501 gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg actgcttagg tatcattggt
     7561 gttgcgcgtg acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg
     7621 gttggtgcga ccatcgacga cacgttgccg attacagtcg aagcgcctga agcctgcccg
     7681 cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg
     7741 aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac
     7801 tatgtactgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc
     7861 ggcattgtgg tgcggatggc aaaagagggc gaaacgctgg tgctgcttga tggcactgaa
     7921 gcgaagctga atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc
     7981 ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa
     8041 tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact
     8101 gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa
     8161 cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc
     8221 accaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag caaactggat
     8281 cgcctgatcg gccatcatat tgctgatgaa caggtaactg acattctgcg tcgtctcggc
     8341 tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg gcgtttcgat
     8401 atggagattg aagaagatct ggttgaagaa gtcgcgcgtg tttacggcta caacaacatc
     8461 ccggatgagc cggtacaggc aagcctgatt atgggtactc accgtgaagc tgacctgtcg
     8521 ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc
     8581 ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca
     8641 agcccgatct ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg cctgctggca
     8701 accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg
     8761 cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt
     8821 gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc
     8881 tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt
     8941 gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg
     9001 aaaggggaac gtattggttt tgttggagtt gttcatcctg aactggaacg taaactggat
     9061 cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg
     9121 cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc
     9181 gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag
     9241 gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag
     9301 agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt
     9361 gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg
     9421 gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg
     9481 gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc
     9541 tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa
     9601 gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg
     9661 cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa
     9721 agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc
     9781 gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac
     9841 ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc
     9901 tgctggcgct tctcttaagc ctttgcgccg gtgaacaatg gatttcgcca ggtgactggt
     9961 ttactcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat
    10021 tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc
    10081 ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg
    10141 cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg
    10201 gcgcgcttat catcacttta atactcttac gtttcgcccg tcgtcatctt tcgaccagtc
    10261 ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg acgtgggcta
    10321 tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg
    10381 gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga
    10441 tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac
    10501 tgggtttacc cctgtggttc tggcgcaatg tgctggtggc agcgaccggc tggatggttg
    10561 gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc
    10621 ttcggttgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg
    10681 cgagcgcagt gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc
    10741 ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag
    10801 caggacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca
    10861 cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga
    10921 agtgaccacg ctggagaagt acgccggtaa tgtgctgttg attgtcaatg tcgcctcaaa
    10981 gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct gggccgatcg
    11041 aggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga
    11101 tgaagagatt aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa
    11161 gattgaagtt aacggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc
    11221 gaccgcagtc gcgccggaag agagcggatt ctatgcccgt atggtcagca aaggtcgtgc
    11281 accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg gcagggacgg
    11341 attggtcatc cagcgttttt ccccggatat gacgccggaa gaccccattg tgatggaaag
    11401 cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa
    11461 tctacccgcc tggggccact ttctggcgag gttcgggctg gggagatcct gcacctggtg
    11521 gggccgaatg gcgcgggtaa gagtacctta ctggcacgaa tggccggaat gaccagcggt
    11581 aagggaagta ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg
    11641 ctgcatcgcg cctatctttc acaacagcag acgccgccgt ttgcaatgcc ggtctggcac
    11701 tatctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg
    11761 gcgctggctc ttgatgacaa actcggacgt agcaccaatc aactttccgg cggtgaatgg
    11821 caacgcgtac gtcttgctgc ggtggtgctg caaatcacac cacaagccaa tcccgcaggc
    11881 caattgctgc ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta
    11941 gacaaaattc tgagcgctct gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat
    12001 ctcaaccaca cattgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg
    12061 gccagtggac gcagagaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg
    12121 aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat
    12181 tagtatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaacagagga
    12241 accgtacgga atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag
    12301 ccaccataaa gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg
    12361 tttgaacgac cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg
    12421 gcgcggtgtg gactgttcgg gatttgtggt tgtaacgatg cgcgatcgtt tcgatttgca
    12481 gctgccccga gaaaccaaag aacaagcctc tatcggcacg caaattgata aagacgagtt
    12541 gctgcctggt gacctggtct ttttcaaaac gggttccgga caaaatggtt tgcatgtagg
    12601 tatttacgat accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc
    12661 ctcacttgat aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa
//
LOCUS       DAZPLM010000116        12636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPLM010000116 DAZPLM010000000
VERSION     DAZPLM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.116"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(116..1399)
                     /gene="gltA"
                     /locus_tag="ACHVC7_002736"
     CDS             complement(116..1399)
                     /gene="gltA"
                     /locus_tag="ACHVC7_002736"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR2116744.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1605..1712)
                     /locus_tag="ACHVC7_002737"
     CDS             complement(1605..1712)
                     /locus_tag="ACHVC7_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116745.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2093..2497
                     /gene="sdhC"
                     /locus_tag="ACHVC7_002738"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2093..2497
                     /gene="sdhC"
                     /locus_tag="ACHVC7_002738"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR2116746.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2491..2838
                     /gene="sdhD"
                     /locus_tag="ACHVC7_002739"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2491..2838
                     /gene="sdhD"
                     /locus_tag="ACHVC7_002739"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR2116747.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2838..4604
                     /gene="sdhA"
                     /locus_tag="ACHVC7_002740"
     CDS             2838..4604
                     /gene="sdhA"
                     /locus_tag="ACHVC7_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR2116748.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4620..5336
                     /gene="sdhB"
                     /locus_tag="ACHVC7_002741"
     CDS             4620..5336
                     /gene="sdhB"
                     /locus_tag="ACHVC7_002741"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR2116749.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5637..8438
                     /gene="sucA"
                     /locus_tag="ACHVC7_002742"
     CDS             5637..8438
                     /gene="sucA"
                     /locus_tag="ACHVC7_002742"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR2116750.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8453..9670
                     /gene="odhB"
                     /locus_tag="ACHVC7_002743"
     CDS             8453..9670
                     /gene="odhB"
                     /locus_tag="ACHVC7_002743"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR2116751.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9764..10930
                     /gene="sucC"
                     /locus_tag="ACHVC7_002744"
     CDS             9764..10930
                     /gene="sucC"
                     /locus_tag="ACHVC7_002744"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR2116752.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     CELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10930..11799
                     /gene="sucD"
                     /locus_tag="ACHVC7_002745"
     CDS             10930..11799
                     /gene="sucD"
                     /locus_tag="ACHVC7_002745"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR2116753.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11903..12625)
                     /gene="mngR"
                     /locus_tag="ACHVC7_002746"
     CDS             complement(11903..12625)
                     /gene="mngR"
                     /locus_tag="ACHVC7_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR2116754.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3030 a         3215 c         3436 g         2955 t
ORIGIN      
        1 tatatgaatg tccgatagtg gattttattt ttacaatttt ttgtgtttgg gcatataaaa
       61 attaacccgc catttgaacg gcgggttaaa atatttacaa cttagcaatc aaccattaac
      121 gcttgatatc gcttttaaag tcgcgttttt catatcctgt atacagctga cgcggacggg
      181 caatcttcat accgtcactg tgcatttcgc tccagtgggc gatccagcca acggtacgtg
      241 ccattgcgaa aatgacggtg aacatggaag acggaatacc catcgctttc aggatgatac
      301 cagagtagaa atcgacgttc gggtacagtt tcttctcgat aaagtacggg tcgttcagcg
      361 cgatgttttc cagctccata gccacttcca gcaggtcatc tttggtgccc agctctttca
      421 gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc
      481 ggtgaccgaa gcccatcagg cggaaagaat catttttatc tttcgcacga cgaacaaatt
      541 ccggaatgtg tttaacggag ctaatttctt ccagcatttt cagcgccgct tcgttagcac
      601 caccgtgcgc aggtccccac agtgaagcaa tacctgctgc gatacaggca aacgggttcg
      661 cacccgaaga gccagcggta cgcacggtgg aggtagaggc gttctgttca tggtcagcgt
      721 gcaggatcag aatacggtcc atagcacgtt ccagaatcgg attaacttca tacggttcgc
      781 acggcgtgga gaacatcata ttcaggaagt taccggcata ggagagatcg ttgcgcggat
      841 aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc ggccatggtc ggcattttcg
      901 acagcaggcg gaacgcggca atttcacggt gacgaggatt gttaacatcc agcgagtcgt
      961 gatagaacgc cgccagcgcg ccggtaatac cacacatgac tgccattgga tgcgagtcgc
     1021 gacggaaagc atggaacaga cgggtaatct gctcgtggat catggtatga cgggtcaccg
     1081 tagttttaaa ttcgtcatac tgttcctgag tcggtttttc accattcagc aggatgtaac
     1141 aaacttccag gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca
     1201 aaataccttc atcaccatca ataaaagtaa ttttagattc gcaggatgcg gttgaagtga
     1261 agcctgggtc aaaggtgaac acaccttttg aaccgagagt acggatatca ataacatctt
     1321 gacccagcgt gcctttcagc acatccagtt caacagctgt gtccccgttg agggtgagtt
     1381 ttgcttttgt atcagccatt taaggtctcc ttagcgcctt attgcgtaag actgccggaa
     1441 cttaaatttg ccttcgcaca tcaacctggc tttacccgtt tgttatttgg ctcgccgctc
     1501 tgtgaaagag gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa aggatccatt
     1561 gatgacgaat aaatggcgaa tcaagtactt agcaatccga attattaaac ttgtctacca
     1621 ctaataactg tcccgaatga attggtcaat acttccacac tgttacataa gttaatctta
     1681 ggtgaaatac cgacttcata acttttacgc attatatgct tttcctggca atgtttgtaa
     1741 caactttgtt gaatgattgt caaattagat gattaaaaat taaataaatg ttgttatcgt
     1801 gacctggatc actgttcagg ataaaacccg acaaactata tgtaggttaa ttgtaatgat
     1861 tttgtgaaca gcctatactg ccgccaggtc tccggaacac cctgcaatcc cgagccaccc
     1921 agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata
     1981 gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg aaatcccgac gtctccaggt
     2041 aacagaaagt taacctctgt gcccgtagtc cccagggaat aataagaaca gcatgtgggc
     2101 gttattcatg ataagaaatg tgaaaaaaca aagacctgtt aatctggacc tacagaccat
     2161 ccggttcccc atcacggcga tagcgtccat tctccatcgc gtttccggtg tgatcacctt
     2221 tgttgcagtg ggcatcctgc tgtggcttct gggtaccagc ctctcttccc ctgaaggttt
     2281 cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct
     2341 taccgctctg gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg attttggcta
     2401 tctggaagaa acattcgaag cgggtaaacg ctccgccaaa atctcctttg ttattactgt
     2461 cgtgctttca cttctcgcag gagtcctcgt atggtaagca acgcctccgc attaggacgc
     2521 aatggcgtac atgatttcat cctcgttcgc gctaccgcta tcgtcctgac gctctacatc
     2581 atttatatgg tcggtttttt cgccaccagt ggcgaactga catatgaagt ctggatcggt
     2641 ttcttcgcct ctgcgttcac caaagtgttc accctgctgg cgctgttttc tatcttgatt
     2701 catgcctgga tcggcatgtg gcaggtgttg accgactacg ttaaaccgct ggcattgcgc
     2761 ctgatgctgc aactggtgat tgtcgttgca ctggtggttt acgtgattta tggattcgtt
     2821 gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt gtgattggtg
     2881 ccggtggcgc aggtatgcgc gcggcgctgc aaatttccca gagcggccag acctgtgcgc
     2941 tgctctctaa agtcttcccg acccgttccc ataccgtttc tgcgcaaggc ggcattaccg
     3001 ttgcgctggg taatacccat gaagataact gggaatggca tatgtacgac accgtaaaag
     3061 ggtcggacta tatcggtgac caggacgcga ttgaatatat gtgtaaaacc gggccggaag
     3121 cgattctgga actcgaacac atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct
     3181 atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg
     3241 cggcggcggc tgaccgtacc ggtcacgcac tgttgcacac gctttatcag cagaacctga
     3301 aaaaccacac caccattttc tccgagtggt atgcgctgga tctggtgaaa aaccaggatg
     3361 gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag
     3421 cccgcgctac cgtgctggcg actggcgggg cagggcgtat ttatcagtcc accactaacg
     3481 cccacattaa cactggcgac ggtgtcggca tggctatccg cgcaggcgta ccggtgcagg
     3541 atatggaaat gtggcaattc cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg
     3601 aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc tttatggagc
     3661 gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga
     3721 ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac
     3781 tcgatcacct gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg gagctttccc
     3841 gtacctttgc tcacgtcgat ccggtgaaag agccgattcc ggttatccca acctgtcact
     3901 acatgatggg cggtattccg accaaagtga ccggtcaggc gctgacggtg aacgagaaag
     3961 gcgaagatgt ggttgttccg gggctgtttg ccgttggtga aatcgcttgt gtatcggtac
     4021 acggcgctaa ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt ggtcgtgcgg
     4081 caggtctgca tctgcaagag tctatcgccg aacagggcgc attgcgcgat gccagcgagt
     4141 ctgatgttga agcatctctg gatcgtctga accgctggaa caataatcgt aacggtgaag
     4201 atccggtggc gattcgtaaa gcgctgcaag aatgtatgca gcataacttc tcggtcttcc
     4261 gtgaaggtga tgcgatggcg aaagggcttg agcagttgaa agtgatccgc gagcgtctga
     4321 aaaatgcccg tctggatgac acttccagcg agttcaacac ccagcgcgtt gagtgcctgg
     4381 aactggataa cctgatggaa acggcgtatg caacggctgt ttctgccaac ttccgtaccg
     4441 aaagccgtgg cgcacatagc cgcttcgact tcccggatcg tgatgatgaa aactggctgt
     4501 gccactccct gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac
     4561 cgaaactgcg cccggcattc ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa
     4621 tgagactcga gttttcaatt tatcgctata acccggatgt tgatgatgct ccgcgtatgc
     4681 aggattacac cctggaagcg gaagaaggtc gcgacatgat gctgctggat gcgcttatcc
     4741 agctgaaaga gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg
     4801 gttccgacgg tctgaacatg aacggcaaga atggtctggc ctgtattacc ccgatttcgg
     4861 cactcaacca gccgggcaag aagattgtga ttcgcccgct gccaggttta ccggtgatcc
     4921 gcgatttggt ggtagacatg ggacaattct atgcgcaata tgagaaaatt aagccttacc
     4981 tgttgaataa tggacaaaat ccgccagctc gcgagcattt acagatgcca gagcagcgcg
     5041 aaaaactcga cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt
     5101 ctttctggtg gaatcccgat aagtttatcg gcccggcagg cttgttagcg gcatatcgtt
     5161 tcctgatcga tagccgtgat accgagactg acagccgcct cgacggtttg agcgatgcat
     5221 tcagcgtatt ccgctgtcac agcatcatga actgcgtcag tgtatgtccg aaggggctga
     5281 acccgacgcg cgccatcggc catattaagt cgatgttgtt gcaacgtaat gcgtaaaccg
     5341 taggcctgat aagacgcgca agcgtcgcat caggcaacca gtgccggatg cggcgtgaac
     5401 gccttatccg gcctacaagt cattacccgt aggcctgata agcgcagcgc atcaggcgta
     5461 acaaagaaat gcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt
     5521 tccttcgcga gccactacat agacaagagc tcgcaagtga accccggcac gcacatcact
     5581 gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc
     5641 agaacagcgc tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct
     5701 ggatagaaca gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc
     5761 gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa
     5821 cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg
     5881 accctgacac caatgtgaag caggttaaag tcctgcagct cattaacgca taccgcttcc
     5941 gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg
     6001 atctggatcc gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg
     6061 gttcatttgc cagcggcaaa gaaaacatga aactcggcga gctgctggaa gccctcaagc
     6121 aaacctactg tggcccgatt ggtgccgagt atatgcacat taccagcacc gaagaaaaac
     6181 gctggatcca acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac
     6241 gcttcttaag cgaactgacc gccgctgaag gccttgaacg ttacctcggc gcaaaattcc
     6301 ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga
     6361 tgatccgcca cgctggcaac agcggcaccc gcgaagtggt tcttgggatg gcgcaccgtg
     6421 gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt
     6481 ttgccggtaa acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct
     6541 cgtctgactt ccagaccgat ggcggcctgg tgcacctggc gctggcgttt aacccgtctc
     6601 accttgagat tgtaagcccg gtagttatcg gttctgttcg tgcccgtctg gacagacttg
     6661 atgagccgag cagcaacaaa gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg
     6721 ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg
     6781 gtacggtacg tatcgttatc aacaaccagg ttggtttcac cacctctaat ccactggatg
     6841 ctcgttctac accgtactgt actgatatcg gtaagatggt tcaggcaccg attttccacg
     6901 ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta
     6961 acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg
     7021 aagccgacga gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga
     7081 caccgcgcaa aatctacgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg
     7141 ccaccgagat ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag
     7201 agtggcgtcc gatgaacatg cactctttca cctggtcgcc gtacctcaac cacgaatggg
     7261 acgaagagta cccgaacaaa gttgagatga agcgcctgca ggagctggcg aaacgcatca
     7321 gcacggtgcc ggaagcagtt gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc
     7381 aggcgatggc tgccggtgag aaactgttcg actggggcgg tgcggaaaac ctcgcttacg
     7441 ccacgctggt tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta
     7501 ccttcttcca ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgt
     7561 tgcaacacat ccataacggg cagggcgcgt tccgcgtctg ggactccgta ctgtctgaag
     7621 aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga accacgcact ctgaccatct
     7681 gggaagcgca gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct
     7741 cttctggcga acagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt
     7801 acgaagggca ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg
     7861 ctgagcaaaa catgcaggtt tgcgttccgt ctaccccggc acaggtttac cacatgctgc
     7921 gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtagt gatgtcgccg aaatccctgc
     7981 tgcgtcatcc gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag
     8041 ccatcggtga aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg
     8101 gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca
     8161 ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc
     8221 agtttgctca cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat
     8281 ggtactgcag ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg
     8341 caggccgcac ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc
     8401 aacaagatct ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag
     8461 cgtagatatt ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg
     8521 gcataaaaaa cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga
     8581 caaagtggta ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga
     8641 tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc
     8701 cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca
     8761 ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc
     8821 tgaacacaat ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgactcg
     8881 tgaagatgtg gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc
     8941 tgctccggcg gcgcaaccgg ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg
     9001 cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac
     9061 cacgttcaac gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc
     9121 gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt
     9181 tgaagccctg aaacgttacc cggaagtgaa tgcttctatc gacggtgatg acgtggttta
     9241 ccacaactat ttcgacgtca gcatggcggt ttccacgccg cgcggcctgg tgacgccggt
     9301 actgcgtgat gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc
     9361 agttaaaggc cgtgacggca agctgacggt tgaagatctg accggtggta acttcaccat
     9421 caccaacggt ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag
     9481 cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga
     9541 gatcctgccg atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga
     9601 atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa gatccgacgc gtctgctgct
     9661 ggacgtgtag tagtttaagc ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc
     9721 tacggtttaa aagataacga ttactgaagg atggacagaa cacatgaact tacatgaata
     9781 tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt atgcctgtac
     9841 tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa
     9901 atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa
     9961 agaagacatc cgtgcttttg cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac
    10021 agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg atatcgctaa
    10081 agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct ttatggcctc
    10141 caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa
    10201 agttgcgctt gatccgctga ctggcccgat gccgtatcag ggatgcgagc tggcgttcaa
    10261 actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg gcctggcgac
    10321 cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca
    10381 gggcgatctg atttgcctcg acggcaaact gggcgctgac ggcaacgcac tgttccgcca
    10441 gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc
    10501 acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc
    10561 aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac cggctaactt
    10621 ccttgacgtt ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca aaatcatcct
    10681 ctctgacgac aaagtgaaag ccgttctggt taacatcttc ggcggtatcg ttcgttgcga
    10741 cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt
    10801 ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg acagcggcct
    10861 gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg ccgcagtgga
    10921 ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca gggctttact
    10981 ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa aatggttggc
    11041 ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt caacaccgtg
    11101 cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc agcaccgttc
    11161 tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat caccatcact
    11221 gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga agccggcgtt
    11281 cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc
    11341 cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc cggtacactg
    11401 acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac ctgtgttggt
    11461 atcggcggtg acccgatccc aggctctaac tttatcgaca tcctcgaaat gttcgaaaaa
    11521 gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa
    11581 gcggcagcgt acatcaaaga gcacgttacc aagccagttg taggttacat cgctggtgtg
    11641 actgcgccga aaggcaaacg tatgggccac gcgggtgcga tcattgctgg tgggaaaggg
    11701 actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg
    11761 gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc
    11821 cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat ggttggccat
    11881 cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ctgcgctatc agggtaaatt
    11941 tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca tcaactaaat
    12001 atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc agacggctca
    12061 tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata accatcttct
    12121 tcacttcttc aataaagtga tatttcgaat tttccatgac ctgccaggtg agatccggga
    12181 acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg cgatagcgca
    12241 cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat
    12301 cgtcagccgg aatcacttca aatatcagga tttcactgtg tgtatcgacg tgacggtccg
    12361 acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc tcttctttga
    12421 cgtaagtccc gctgccctga atgctttcga ggatctgctg ctcgactagc tggcgcaaag
    12481 cctgacgcac cgtaacccgg ctgacgccaa actctgtttg tagcgctgat tcagtgggta
    12541 acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga tcggcaatct
    12601 gccggtataa gggcttgtgt cccattttta gtatct
//
LOCUS       DAZPLM010000117        12545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPLM010000117 DAZPLM010000000
VERSION     DAZPLM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.117"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHVC7_002747"
     CDS             complement(<1..330)
                     /locus_tag="ACHVC7_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR2116755.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHVC7_002748"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHVC7_002748"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR2116756.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHVC7_002749"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHVC7_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR2116757.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHVC7_002750"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHVC7_002750"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR2116758.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHVC7_002751"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHVC7_002751"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR2116759.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHVC7_002752"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHVC7_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR2116760.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHVC7_002753"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHVC7_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR2116761.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHVC7_002754"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHVC7_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR2116762.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHVC7_002755"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHVC7_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR2116763.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHVC7_002756"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHVC7_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR2116764.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHVC7_002757"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHVC7_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR2116765.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHVC7_002758"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHVC7_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR2116766.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHVC7_002759"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHVC7_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR2116767.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2884 a         3491 c         3059 g         3111 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagggcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgta
//
LOCUS       DAZPLM010000118        12504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPLM010000118 DAZPLM010000000
VERSION     DAZPLM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.118"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHVC7_002760"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHVC7_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR2116768.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHVC7_002761"
     CDS             433..621
                     /locus_tag="ACHVC7_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR2116769.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHVC7_002762"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHVC7_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR2116770.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHVC7_002763"
     CDS             816..989
                     /locus_tag="ACHVC7_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR2116771.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHVC7_002764"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHVC7_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHVC7_002765"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHVC7_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR2116772.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHVC7_002766"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHVC7_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR2116773.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHVC7_002767"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHVC7_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR2116774.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHVC7_002768"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHVC7_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR2116775.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHVC7_002769"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHVC7_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHVC7_002770"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHVC7_002770"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR2116776.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHVC7_002771"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHVC7_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR2116777.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHVC7_002772"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHVC7_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR2116778.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3016 a         3205 c         3273 g         3010 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgtggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc atta
//
LOCUS       DAZPLM010000119        12439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPLM010000119 DAZPLM010000000
VERSION     DAZPLM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.119"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHVC7_002773"
     CDS             complement(<1..70)
                     /locus_tag="ACHVC7_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116779.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="ACHVC7_002774"
     CDS             689..1459
                     /locus_tag="ACHVC7_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR2116780.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="ACHVC7_002775"
     CDS             1450..1923
                     /locus_tag="ACHVC7_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR2116781.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHVC7_002776"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHVC7_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR2116782.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHVC7_002777"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHVC7_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR2116783.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHVC7_002778"
     CDS             3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHVC7_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR2116784.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="ACHVC7_002779"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="ACHVC7_002779"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR2116785.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHVC7_002780"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHVC7_002780"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR2116786.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="ACHVC7_002781"
     CDS             7698..8612
                     /locus_tag="ACHVC7_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR2116787.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10188
                     /locus_tag="ACHVC7_002782"
     CDS             8827..10188
                     /locus_tag="ACHVC7_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2116788.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHVC7_002783"
                     /pseudo
     CDS             complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHVC7_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12011..>12439)
                     /gene="hpaR"
                     /locus_tag="ACHVC7_002784"
     CDS             complement(12011..>12439)
                     /gene="hpaR"
                     /locus_tag="ACHVC7_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR2116789.1"
                     /translation="IALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDFHD
                     LAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEE
                     AYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3061 a         3268 c         2992 g         3118 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
     9961 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
    10021 gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc
    10081 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
    10141 tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc
    10201 cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc
    10261 gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga
    10321 tgtttcacgc tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc
    10381 ctgctcttcc agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg
    10441 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10501 atcagaagca gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat
    10561 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10621 cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt
    10681 acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc
    10741 aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat
    10801 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10861 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg
    10921 gcgcgcattt tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt
    10981 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc
    11041 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    11101 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    11161 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    11221 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    11281 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    11341 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    11401 ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    11461 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11521 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11581 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11641 accgctacca atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11701 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt
    11761 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga
    11821 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11881 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11941 aaaactttat gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga
    12001 tactaaaaag ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa
    12061 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    12121 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    12181 tggttagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    12241 cgtcgcgttc catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    12301 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    12361 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc
    12421 gcgcctggag caacgcaat
//
LOCUS       DAZPLM010000120        12080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPLM010000120 DAZPLM010000000
VERSION     DAZPLM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.120"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHVC7_002785"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHVC7_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR2116790.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHVC7_002786"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHVC7_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR2116791.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHVC7_002787"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHVC7_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR2116792.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHVC7_002788"
     CDS             complement(3668..3898)
                     /locus_tag="ACHVC7_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR2116793.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHVC7_002789"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHVC7_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHVC7_002790"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHVC7_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR2116794.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHVC7_002791"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHVC7_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHVC7_002792"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHVC7_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR2116795.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHVC7_002793"
     CDS             7629..8642
                     /locus_tag="ACHVC7_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2116796.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHVC7_002794"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHVC7_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHVC7_002795"
     CDS             9533..10327
                     /locus_tag="ACHVC7_002795"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2116797.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHVC7_002796"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHVC7_002796"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR2116798.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="ACHVC7_002797"
     CDS             11983..>12080
                     /locus_tag="ACHVC7_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR2116799.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2946 a         2913 c         3223 g         2998 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaatc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAZPLM010000121        11986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPLM010000121 DAZPLM010000000
VERSION     DAZPLM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.121"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..358
                     /locus_tag="ACHVC7_002798"
                     /pseudo
     CDS             1..358
                     /locus_tag="ACHVC7_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            681..2069
                     /gene="narU"
                     /locus_tag="ACHVC7_002799"
     CDS             681..2069
                     /gene="narU"
                     /locus_tag="ACHVC7_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR2116800.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2151..5891
                     /gene="narZ"
                     /locus_tag="ACHVC7_002800"
     CDS             2151..5891
                     /gene="narZ"
                     /locus_tag="ACHVC7_002800"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR2116801.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5888..7432
                     /gene="narH"
                     /locus_tag="ACHVC7_002801"
     CDS             5888..7432
                     /gene="narH"
                     /locus_tag="ACHVC7_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR2116802.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7432..8127
                     /gene="narW"
                     /locus_tag="ACHVC7_002802"
     CDS             7432..8127
                     /gene="narW"
                     /locus_tag="ACHVC7_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR2116803.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8124..8804
                     /gene="narI"
                     /locus_tag="ACHVC7_002803"
     CDS             8124..8804
                     /gene="narI"
                     /locus_tag="ACHVC7_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR2116804.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYACFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8883..9776
                     /locus_tag="ACHVC7_002804"
     CDS             8883..9776
                     /locus_tag="ACHVC7_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR2116805.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9872..10717)
                     /gene="nhoA"
                     /locus_tag="ACHVC7_002805"
     CDS             complement(9872..10717)
                     /gene="nhoA"
                     /locus_tag="ACHVC7_002805"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR2116806.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10890..11459
                     /locus_tag="ACHVC7_002806"
     CDS             10890..11459
                     /locus_tag="ACHVC7_002806"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR2116807.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11463..11690)
                     /gene="pptA"
                     /locus_tag="ACHVC7_002807"
     CDS             complement(11463..11690)
                     /gene="pptA"
                     /locus_tag="ACHVC7_002807"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR2116808.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11790..>11986)
                     /locus_tag="ACHVC7_002808"
     CDS             complement(11790..>11986)
                     /locus_tag="ACHVC7_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2116809.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMGRNYLASVLTGSGLS"
BASE COUNT         2849 a         3080 c         3118 g         2939 t
ORIGIN      
        1 gggaagaaca attgccctac tatgcagctc atgttattta atttgttttc cccaacactt
       61 aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga
      121 atagatgtgg ataacgaatt gtttcactat cctattaaaa atagcatata ccccatataa
      181 tatacacact tacaagacag aggaagttgt aaaccagagg aatataaaag ttaaaaacat
      241 gaccttagat gaaataaaca atacttactg taataacgat tattacaatc aggcaataag
      301 agaggaaccg atagaccttc tggacagatc gttttcctcc agttcatggc ctttttagtg
      361 aataaatcat cactaattct ataagtgtct ggtcttgttg ataatatagt taatatgtaa
      421 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaaccccgt
      481 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      541 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      601 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      661 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      721 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      781 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      841 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      901 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      961 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     1021 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
     1081 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
     1141 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     1201 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1261 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1321 catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1381 atatcgccag ttcacgcgcc tcaattgacg accagctccc tgtcttacaa cgcctgcatc
     1441 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1501 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1561 ttggcccatt tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1621 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1681 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1741 ggctgtttct gactgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1801 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1861 aagaagccat caccgaaacg gcggcggctc tgggctttat ctcagccatt ggcgcagtgg
     1921 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg
     1981 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     2041 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc
     2101 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     2161 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     2221 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2281 aaatcgtgcg ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg
     2341 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2401 accagcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatctct
     2461 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgtg
     2521 aagctctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc
     2581 aaaagtgctt aagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2641 aggaacttaa ccaactgatt gccgccgcta acgtctggac catcaaaatc tatggtccgg
     2701 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtgtcatac gctgccggaa
     2761 cgcgttatct gtcgctgtta ggcggcacct gtttaagctt ctacgactgg tattgcgacc
     2821 tgccgcccgc ctcgccgatg acctggggcg aacaaaccga cgtgccggaa tctgccgact
     2881 ggtataactc cagttacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg
     2941 acgcccactt ctttaccgaa gtacgttaca aaggcaccaa aactatcgcc attacccctg
     3001 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     3061 gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca
     3121 gcgactactt tatcaactac tgccgccgct atagcgacat gccgatgctg gtaatgctgg
     3181 aacctcgcga cgatggtagc tacgtacccg ggcgcatgat ccgcgcgtct gacctggtag
     3241 atggactagg cgaaagcaat aatccgcagt ggaaaaccgt ggcggttaat accgcaggtg
     3301 aattggtagt gccgaatggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3361 tggaatccat tgccgccggt aaggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3421 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3481 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3541 atggcaacac ctgtccggtg gtcagcgttt atgatttggt gctggcgaat tacggcctcg
     3601 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3661 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3721 aatttgccga taccgcccat aaaacgcatg gacgctcaat gattatcctc ggcgctggcg
     3781 tcaaccactg gtatcacatg gatatgaact accgtgggat gatcaatatg ctgatcttct
     3841 gcggttgtgt cgggcaaagc ggcggcggct gggcgcacta tgtcggtcag gaaaaactgc
     3901 gcccacaaac cggctggttg ccgctggcct ttgcgctcga ctggaaccgc ccaccgcgcc
     3961 agatgaacag tacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     4021 ctgctcagga gttactttca ccgcttgccg atgccagtaa gtacagcggt catctgattg
     4081 atttcaacgt tcgcgccgaa cgcatgggct ggctaccttc tgcgccgcag ctggggcgta
     4141 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     4201 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4261 atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc
     4321 acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4381 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4441 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4501 tcgttctgcc caccgccacc tggtacgaaa aagacgatat gaacacctcg gatatgcatc
     4561 cgtttattca tccgctttct gcggcggtcg atccggcgtg ggaatcacgc agcgactggg
     4621 aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag
     4681 aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt
     4741 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga
     4801 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4861 cattgatgga caaacttggc aatggtggta aagggatttc gtggaatacc caggatgaaa
     4921 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4981 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     5041 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     5101 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     5161 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgtc tcctataatg
     5221 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5281 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5341 ctatcgacac ccgtagcgtc agtgagatgc gccagatccc gccaaacggc ttcccggaaa
     5401 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5461 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaaccgatg
     5521 cccgagaact gaccattgtc gataacgact gggtggaagt ctttaacgcc aacggcgcgc
     5581 tgacggctcg tgcggtggtc agccaacgtg taccgccggg tatgaccatg atgtatcacg
     5641 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5701 ataactcggt cacccgtgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5761 tggcctgggg ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga
     5821 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5881 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgaca aatgtatcgg
     5941 ttgccatacc tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta
     6001 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     6061 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatac gcccgcgtct
     6121 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagattga
     6181 tgattactac gaacctttca ccttcgacta cgaacatttg catagcgcac cggaaggcaa
     6241 acatatccct actgctcgcc cgcgttcgct gattgacggt aagcggatgg acaaagtgat
     6301 ctgggggcca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     6361 caacttcgag gccatgcaaa aggagatgta cgggcagttt aaaaacacct tcatgatgta
     6421 cctgccgcgc ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct gcccaagcgg
     6481 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     6541 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     6601 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     6661 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     6721 cattgaggaa gcggcgagca ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt
     6781 gttcctcgat ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag gtattccaca
     6841 aaacgtgatt gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg actggaaact
     6901 ggcgctaccg ctgcaccctg aatatcgcac cctgccaatg gtctggtacg ttcctccgct
     6961 gtcgccgatt cagtcctacg cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc
     7021 cgccatcgaa agcctgcgta ttccggtgca atatctcgcc aatatgttga gtgccggcga
     7081 taccggtccg gtactgcggg cgctgaaacg gatgatggcg atgcgccact atatgcgttc
     7141 acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc
     7201 ccaggtcgaa gaaatgtatc gctatctcgc cattgccaac tatgaagatc gttttgttat
     7261 cccgaccagc catcgggaaa tggcgggcga tgccttcgca gaacgcaacg gctgcggttt
     7321 tacctttggc gacggttgcc acggttcgga cagtaaattc aacctgttca acagtagccg
     7381 tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc
     7441 ctcaaagtga tcggcctgtt gatggagtat ccggatgagc tgttgtggga atgcaaggag
     7501 gacgcgctgg cgttgatccg ccgcgacgcg ccgatgctta cggatttcac ccacaacctg
     7561 cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg aagtgtttga ccgcgggcgc
     7621 accacgtcgc tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg cggccaggca
     7681 atggtggacc tgctggcgga gtatgaaaag gtcggcctgc agctggattg tcgggaactg
     7741 cccgattatc taccgctgta tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa
     7801 gaaggattgc tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc
     7861 gaggcaccgt ggtacgcgtt gtttgatgct ctgctgcaac tggcgggaag tatactttca
     7921 agtgacagtg tcacaaaaca agtaaacagc gaagagcgcg atgacacccg ccaggcgctt
     7981 gatgcagtgt gggaagagga acaggtgaag tttattgaag ataacgccac ggcgtgtgac
     8041 agctcgccgc ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc gcagtatgtc
     8101 gacatcagtg cgggaggtgg gaaatgattc agtatctgaa cgtctttttt tacgatatct
     8161 acccgtacat ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac gactacgggc
     8221 agtacacctg gcgcgcctcc tcaagtcaaa tgctcgataa acgcgggatg gtgatatggt
     8281 cgaatctgtt ccatatcggc attttgggga ttttcttcgg gcacttgttc ggcatgttaa
     8341 cgccgcactg gatgtacgcg tgttttttgc cagtggcagc gaaacaactc atggcgatgg
     8401 tgctcggcgg tatttgcggt gtgttgacgt tgattggcgg cgcaggactg ctgtggcgca
     8461 gtctgacaaa tcagcgcgtg cgtgccactt ccactacgcc ggatatcatc atcatgagca
     8521 ttttgctgat ccagtgtctg ttggggctaa gtaccatacc gttttcagca cagtatcctg
     8581 atggtagcga aatgatgaag ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg
     8641 gatcgtcaga gatgctcaac ggcgtagcgt tcgtcttccg cctgcatctg gtgttgggaa
     8701 tgaccatctt cctgctcttc ccgttcaccc gactggtgca tgtgtggagc gcgccgtttg
     8761 agtactttac tcgtcgatat caaattgtgc gttcgcggcg ataatttcac cctggccgga
     8821 tagcccttcg ctctccggcc acttgcaggc tatgatagtt ctccagtcac aggagaacgt
     8881 taatgaaacc gcaggtgtac cacgtcgatg cttttacctc tcaaccattt cgcggcaatt
     8941 ctgccggtgt tgttttcccc gccgataatc ttagcgaagc acaaatgcag cttatcgccc
     9001 gcgagttagg ccattcggaa actgcttttc tgctgcacag cgacgacagc gatgtgcgca
     9061 tccgctactt tacgccaacg gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc
     9121 actatgtacg tgcgaaggtg ttaggtttag gaaattgcac gatctggcaa acatcgctgg
     9181 caggaaaaca tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt tcgctggaac
     9241 aaggtacgcc gggctttgaa ctgccgctgg agggtgaagt acgcgcggcg attatcaacg
     9301 cattgcatct tactgaagac gatattctgc cgggtttgcc gatccaggtc gcgacgaccg
     9361 ggcattctaa agtgatgatc ccattgaagc cggaagtgga tatcgacgct ctttcgcctg
     9421 accttaatgc gctgaccgct atcagtaaac agattggctg caatggtttc ttcccgttcc
     9481 agatccgtcc aggcaaaaac gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg
     9541 tggaagatcc ggtgactggc aatgccaatg gcccgatggg tgcatggttg gtacatcaca
     9601 acgtattgcc ccacgatggc aacgtgttgt gtgttaaagg ccatcagggg cgcgcattgg
     9661 ggcgtgacgg catgattgaa gtgacggtga caattcgtga taaccaaccg gaaaaagtca
     9721 ccatttcagg cacagcggtg attttgtttc atgccgaatg ggcaattgaa atctgaaatc
     9781 gttgcctgat gcgcttcgcc tgtcaggtct acaaccgggc taatgtatgc cggataaggc
     9841 gtttacgccg catccggcaa cctgacataa attattttcc cgcctccgga tgcgtatcaa
     9901 acgccgccat caccagcgct aactcatcca cagtaaagcc atgtttcgca tcatccacgc
     9961 ccagaccaaa ctgttcctgc atcacggcat ataacgacgc cacatccggt agatttcgct
    10021 gctccaccgc gtgcccattt tcataatggg taaaatgaaa attggtcagt gtcagcttgc
    10081 cgccgtccgg caaatggcgg cacatcagca aatgatggcg aaaatgcgac tgcggccagt
    10141 gcgccgacca gaaattgccc atcacataat cgctttgttg ctgctcgcag agatcgaagc
    10201 ggtacatcgg ctgccaatgc tgatgatgat taaactgcaa cacccaatca tcaccctcct
    10261 gcaacaaccg atactctccg tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg
    10321 gcgcggttag cgtctgccca ccgaaaccga catcagcaat ccatttttcc ccttccagtt
    10381 ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat aacactacgc
    10441 gccctaacaa gctgcgaacg ttaaacccca gctcgcgtaa cacccgctca aacacgccat
    10501 tctgctcaaa acagtaaccg ccacgacggg ctatcaccag tttctcttcc agcgattgat
    10561 catcaagctg tatttccctc ggcagcaaaa cgtcaaggtt ttcaaacgga atggtgcaat
    10621 tgtgtttcag gtgcaatgca cgaagcgtat caatattgac cgcagcagct cccgaccagt
    10681 taatacgggc aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt
    10741 ctcaacttat aacctggaat agacgccgca ccggctattt tgtgcgatcg cggacatgca
    10801 tcacgtttgc aaatcgccaa tcccgttaat ttcgttactt attgcgttac ggactacgct
    10861 taaaaaaacc ttcttcgcga ggcgatacca tgagccgatt catccccata gaattacacc
    10921 atgccagccg tctgttaaat catggcccaa ctgtcttgat caccagtttc gatgagcaat
    10981 cccagcggcg taacattatg gctgcagcct ggtcaatgcc ggtggagttt gaacctccac
    11041 gcgtggcaat tgtggtagat aaatcgacat ggaccaggga gttgattgaa cgtaacggta
    11101 agtttggcat cgttatcccg ggcgttgcag caactaactg gacgtgggcg gtgggaagtg
    11161 tgtcggtgcg tgaagaagat aaatttaatt gctatggcat tccggtggtg aaaggtccgg
    11221 tgcttggttt gccacttgtt gaagagaaat gcctggcgtg gatggagtgc cgcctgctac
    11281 ctgcgacttc tgcgcaacaa aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg
    11341 cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt
    11401 tgcatcattt aggtgctggc acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag
    11461 cattacatgc tgtaaccggg tttctttatc aaagcctcca tttggggctc gatttcggca
    11521 tcccagatag cttgccaaga ttctggttga atctgctgta gagcaatgct tatcgagcta
    11581 tctttactgt tcagatggcg aataataacg tcggtaatat ctgcagcaag tgctgctttt
    11641 tgttgttcgt ccagttcacg cggaaagcat ttaatgtcga tgtgcggcat gattacgctc
    11701 cttttgaaag atgtcagtaa aattaacaca cattgtgaat gtcatgtgtc cggtaaagcg
    11761 tttatacgac aataatgccc agggcaagat tacgaaagcc cgctccccgt aaggactgac
    11821 gccaggtagt ttctgcccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat
    11881 accttatcat tcgtcaaaat attaatagca atgtgccgta tccctgaaaa taattctgct
    11941 gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgt
//
LOCUS       DAZPLM010000122        11946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPLM010000122 DAZPLM010000000
VERSION     DAZPLM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.122"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHVC7_002809"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHVC7_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR2116810.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHVC7_002810"
     CDS             complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHVC7_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR2116811.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLM"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHVC7_002811"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHVC7_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR2116812.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHVC7_002812"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHVC7_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR2116813.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHVC7_002813"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHVC7_002813"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR2116814.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHVC7_002814"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHVC7_002814"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR2116815.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHVC7_002815"
     CDS             complement(6574..7338)
                     /locus_tag="ACHVC7_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2116816.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHVC7_002816"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHVC7_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR2116817.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHVC7_002817"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHVC7_002817"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR2116818.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHVC7_002818"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHVC7_002818"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR2116819.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         2797 a         3347 c         3166 g         2636 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctacatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcg
//
LOCUS       DAZPLM010000123        11911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPLM010000123 DAZPLM010000000
VERSION     DAZPLM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.123"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            68..1177
                     /gene="livK"
                     /locus_tag="ACHVC7_002819"
     CDS             68..1177
                     /gene="livK"
                     /locus_tag="ACHVC7_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR2116820.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1225..2151
                     /gene="livH"
                     /locus_tag="ACHVC7_002820"
     CDS             1225..2151
                     /gene="livH"
                     /locus_tag="ACHVC7_002820"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR2116821.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2148..3425
                     /gene="livM"
                     /locus_tag="ACHVC7_002821"
     CDS             2148..3425
                     /gene="livM"
                     /locus_tag="ACHVC7_002821"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR2116822.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3422..4189
                     /gene="livG"
                     /locus_tag="ACHVC7_002822"
     CDS             3422..4189
                     /gene="livG"
                     /locus_tag="ACHVC7_002822"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR2116823.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4191..4904
                     /gene="livF"
                     /locus_tag="ACHVC7_002823"
     CDS             4191..4904
                     /gene="livF"
                     /locus_tag="ACHVC7_002823"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR2116824.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5188..6252
                     /gene="ugpB"
                     /locus_tag="ACHVC7_002824"
                     /pseudo
     CDS             5188..6252
                     /gene="ugpB"
                     /locus_tag="ACHVC7_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6350..7237
                     /gene="ugpA"
                     /locus_tag="ACHVC7_002825"
     CDS             6350..7237
                     /gene="ugpA"
                     /locus_tag="ACHVC7_002825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR2116825.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7234..8079
                     /gene="ugpE"
                     /locus_tag="ACHVC7_002826"
     CDS             7234..8079
                     /gene="ugpE"
                     /locus_tag="ACHVC7_002826"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR2116826.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8081..9151
                     /gene="ugpC"
                     /locus_tag="ACHVC7_002827"
     CDS             8081..9151
                     /gene="ugpC"
                     /locus_tag="ACHVC7_002827"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR2116827.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9148..9891
                     /gene="ugpQ"
                     /locus_tag="ACHVC7_002828"
     CDS             9148..9891
                     /gene="ugpQ"
                     /locus_tag="ACHVC7_002828"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR2116828.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(9878..10318)
                     /locus_tag="ACHVC7_002829"
     CDS             complement(9878..10318)
                     /locus_tag="ACHVC7_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR2116829.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10438..>11911
                     /gene="ggt"
                     /locus_tag="ACHVC7_002830"
     CDS             10438..>11911
                     /gene="ggt"
                     /locus_tag="ACHVC7_002830"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR2116830.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         2569 a         3068 c         3452 g         2822 t
ORIGIN      
        1 agaacagtct gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt
       61 tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc
      121 acacaccgct atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat
      181 tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc
      241 caaaggggga attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc
      301 gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg
      361 tcatctgtgt tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct
      421 gatgatctcg ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat
      481 gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac
      541 ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc
      601 gcgttcggtg caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat
      661 taccgccggg gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga
      721 cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc
      781 cgttggcctg aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa
      841 tattgctggc gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga
      901 tccggcaaac cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc
      961 ttatgtctgg atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg
     1021 cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat
     1081 tgggccgctg aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca
     1141 gtggcacgcc gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc
     1201 gggcgggttt agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg
     1261 tttaacggcg tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt
     1321 tacggcatta tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat
     1381 gtctccttta tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg
     1441 gtggcggcgg gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa
     1501 cgggtagcct atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc
     1561 ggcatgtcca tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg
     1621 ctgcccagtc tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct
     1681 attaccacca tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg
     1741 attttcattc gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa
     1801 atggcgagcc tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc
     1861 gcggcgatgg cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc
     1921 tatatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc
     1981 agcattccag gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct
     2041 gcctatctga gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg
     2101 ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc
     2161 atattgcaat ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg
     2221 gcgtgcaact ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt
     2281 ggcagtgggt gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt
     2341 tccagaaagg gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct
     2401 ccacggtgaa gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt
     2461 ttatggtgtc acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg
     2521 gtctcgggct gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt
     2581 tttacgccat cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct
     2641 ggacctgttt gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc
     2701 cggtgttgcg tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg
     2761 tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga
     2821 tcccgaaacc gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg
     2881 acacgttcag taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt
     2941 atctggtggc gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga
     3001 tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg
     3061 gcttaagccc gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt
     3121 ttgccggaac gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg
     3181 ctgaatcggc gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg
     3241 tgattctggc ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat
     3301 acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct
     3361 tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg
     3421 catgagtcag ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc
     3481 ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa
     3541 cggtgccgga aaaactacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg
     3601 caccatttta ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat
     3661 gggcgtggtg cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa
     3721 cctgctggtg gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac
     3781 gccttctttc cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg
     3841 cattggtttg ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg
     3901 ccgtctggag attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc
     3961 ggcggcaggt cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg
     4021 caatcatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat
     4081 ttcagaccga atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca
     4141 aatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag
     4201 tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg
     4261 tgagcctgca tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga
     4321 aaaccacctt gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt
     4381 ttgatgataa agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga
     4441 ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg
     4501 gcggcttttt tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt
     4561 tcccacgtct gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac
     4621 aaatgctggc gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc
     4681 catcgcttgg tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc
     4741 gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg
     4801 cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac
     4861 tactggcgaa tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat
     4921 aaggagtcaa aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg
     4981 cttatcaggc ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga
     5041 taaggcgttt acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga
     5101 ccttctattt ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac
     5161 cgccttgtca tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg
     5221 atggcatcga aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc
     5281 gatgagtcgc agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac
     5341 ttactctccc agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc
     5401 aagaaagcgg ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct
     5461 tccgcaaaac tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg
     5521 atccaactgg aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc
     5581 tttgacggca cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc
     5641 atgctcgaag agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc
     5701 accgagaagt tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct
     5761 aatattcgtg agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat
     5821 gcgaaagatg caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc
     5881 aaaaataaag aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa
     5941 aacgctgccg agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac
     6001 ctgacccgtg agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag
     6061 atgctgaata agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag
     6121 atccgcgtga ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag
     6181 caggcactgg ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg
     6241 acgaagtctt aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg
     6301 taggccggat aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc
     6361 ccgtccggtg ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat
     6421 caccgttatc ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt
     6481 cgatccgttt ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca
     6541 tgacagctac tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt
     6601 cagcggtttg ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag
     6661 ccgtttctat caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt
     6721 attgtggatc ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt
     6781 cggctacgac tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc
     6841 ctcggtgtgg aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat
     6901 tccccgttcg ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt
     6961 taagattgct ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt
     7021 gtacgccttc ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca
     7081 ggccaccact acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc
     7141 ttcgtctgcc gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca
     7201 gttccgctat gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg
     7261 atattcagcc ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg
     7321 gcgtttgtcg cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc
     7381 cccggcacac atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat
     7441 agcgcgccgt tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc
     7501 ggcaaaatta ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta
     7561 cgtaacctct tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc
     7621 ttcccgacgg tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg
     7681 ctgccgctga tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta
     7741 ccggatgaat tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc
     7801 gacatcgttt ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc
     7861 tacggctgga atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc
     7921 accgtggcag ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg
     7981 gtaatggcgg cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag
     8041 cgtgccttcg tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca
     8101 ggcagtaacc aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt
     8161 ggcggatggc gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct
     8221 gcgcatggtt gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg
     8281 cgtgaccgaa atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct
     8341 ttatccgcat atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg
     8401 caagcagcaa attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct
     8461 gcttaagcgt cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg
     8521 cgcgattgtg cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc
     8581 caagctgcgc gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac
     8641 ttcactctac gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt
     8701 gatgaacggt ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc
     8761 cagcctgttt gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt
     8821 gaataatgaa ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg
     8881 ctaccgtcag tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct
     8941 aagctcgcag gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc
     9001 agataacctg gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca
     9061 ggagcgcccg acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct
     9121 ttttgatggt gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct
     9181 catcgtggcg gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca
     9241 aaatacggtc ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc
     9301 ctgctccatg acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg
     9361 aactggcagg atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt
     9421 gagccgctgc cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg
     9481 aatatcgaaa tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg
     9541 gcggcacgcc aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat
     9601 gctttagaag ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag
     9661 tggcgcgacg actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat
     9721 cataagttac tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg
     9781 gtttataccg tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc
     9841 atctgtaccg atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg
     9901 gaatgtcagg ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca
     9961 gcatatgctg ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt
    10021 tggtgttggg cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca
    10081 cccgctgaga attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg
    10141 ttttcagttg ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt
    10201 gcatccgctg ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa
    10261 tgggctgtgc aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg
    10321 cgtatcctcc tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa
    10381 ttcagagtta tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg
    10441 ataaaaccga cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt
    10501 agcgccgccg ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc
    10561 cacccggtac gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg
    10621 ggggtggata tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac
    10681 gcgctggcgg taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc
    10741 cgctcgaaaa atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg
    10801 acccgcgata tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg
    10861 catctggctt ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac
    10921 ggcaccatgc cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt
    10981 atcgttaacg acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat
    11041 cacgaaaaca gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg
    11101 ctggtgcagg cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa
    11161 ttctataaag gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg
    11221 atcactaaag aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat
    11281 tatcgcgggt atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta
    11341 caaatcctca atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat
    11401 gcgatgcaaa tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt
    11461 ggcgacccgg attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa
    11521 tccattgccg atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc
    11581 aagcttgcgc cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt
    11641 aacgcggtgg cggtgaccta tacgctgaac accaccttcg gtatgggcat tgtcgcgggc
    11701 gagagcggta ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg
    11761 aacatttacg ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg
    11821 tcatcgatgt caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt
    11881 cctggcggga gtcggattat taccaccgtg c
//
LOCUS       DAZPLM010000124        11842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPLM010000124 DAZPLM010000000
VERSION     DAZPLM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.124"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(83..346)
                     /gene="cedA"
                     /locus_tag="ACHVC7_002831"
     CDS             complement(83..346)
                     /gene="cedA"
                     /locus_tag="ACHVC7_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR2116831.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            529..2790
                     /gene="katE"
                     /locus_tag="ACHVC7_002832"
     CDS             529..2790
                     /gene="katE"
                     /locus_tag="ACHVC7_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR2116832.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3048..3806)
                     /gene="chbG"
                     /locus_tag="ACHVC7_002833"
     CDS             complement(3048..3806)
                     /gene="chbG"
                     /locus_tag="ACHVC7_002833"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR2116833.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3819..5171)
                     /gene="celF"
                     /locus_tag="ACHVC7_002834"
                     /gene_synonym="chbF"
     CDS             complement(3819..5171)
                     /gene="celF"
                     /locus_tag="ACHVC7_002834"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR2116834.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5276..6118)
                     /gene="chbR"
                     /locus_tag="ACHVC7_002835"
     CDS             complement(5276..6118)
                     /gene="chbR"
                     /locus_tag="ACHVC7_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR2116835.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6126..6476)
                     /gene="chbA"
                     /locus_tag="ACHVC7_002836"
     CDS             complement(6126..6476)
                     /gene="chbA"
                     /locus_tag="ACHVC7_002836"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR2116836.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6527..7885)
                     /gene="chbC"
                     /locus_tag="ACHVC7_002837"
     CDS             complement(6527..7885)
                     /gene="chbC"
                     /locus_tag="ACHVC7_002837"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR2116837.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(7970..8290)
                     /gene="chbB"
                     /locus_tag="ACHVC7_002838"
     CDS             complement(7970..8290)
                     /gene="chbB"
                     /locus_tag="ACHVC7_002838"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR2116838.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8589..8927)
                     /gene="osmE"
                     /locus_tag="ACHVC7_002839"
     CDS             complement(8589..8927)
                     /gene="osmE"
                     /locus_tag="ACHVC7_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR2116839.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9129..9956
                     /gene="nadE"
                     /locus_tag="ACHVC7_002840"
     CDS             9129..9956
                     /gene="nadE"
                     /locus_tag="ACHVC7_002840"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR2116840.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10186..11073
                     /gene="cho"
                     /locus_tag="ACHVC7_002841"
     CDS             10186..11073
                     /gene="cho"
                     /locus_tag="ACHVC7_002841"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR2116841.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11033..11608)
                     /gene="ves"
                     /locus_tag="ACHVC7_002842"
     CDS             complement(11033..11608)
                     /gene="ves"
                     /locus_tag="ACHVC7_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR2116842.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3133 a         3040 c         2705 g         2964 t
ORIGIN      
        1 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcatgt aactcccggc
       61 aaaaccggga agtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
      121 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
      181 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
      241 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
      301 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
      361 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
      421 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
      481 tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat
      541 aacgaaaaga acctacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
      601 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
      661 ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa
      721 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
      781 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
      841 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
      901 cgtattgttc atgcacgcgg atcagcagct cacggttatt tccagccata taaaagctta
      961 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
     1021 aggttctcta ccgttcaggg tggcgctggc tctgcagata ccgtgcgtga tatccgtggt
     1081 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
     1141 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
     1201 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
     1261 ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
     1321 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
     1381 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
     1441 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
     1501 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
     1561 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
     1621 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
     1681 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
     1741 gatccgctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
     1801 gggccgaatt tccatgagat tccgattaac cgtccgacct gcccttacca caacttccag
     1861 cgtgatggca tgcatcgtat ggggatcgac actaacccgg cgaattacga accgaactcg
     1921 atcaacgata actggccgcg cgaaacaccg ccgggaccga aacgcggcgg ttttgaatca
     1981 tatcaggaac gcgtggaagg aaataaagtt cgtgagcgca gtccatcgtt tggcgaatat
     2041 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
     2101 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
     2161 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
     2221 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
     2281 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg
     2341 attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccctgaag
     2401 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
     2461 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
     2521 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactac
     2581 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
     2641 aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc tgacagcgct
     2701 gacggtagtt ttatggatga actgctaacg ctgatggcag cacaccgcgt gtggtcacgc
     2761 attcctaaga ttgacaaaat tcctgcctga tgggaggcgg cgcaattgcg ccgcctcaaa
     2821 tgatttacat agtgcgcttt gtttatgccg gatgcggcgt gaacgcctta tccggcctac
     2881 aaaactgtgc aaattcaata tattgcagga aacacgtagg cctgataagc gaagcgcatc
     2941 aggcagtttt gcgtttgtca gcagtctcaa gcggcgcagt tacgccgcct ttgtaggaat
     3001 taatcgccgg atgcaaggtt cacgccgcat ctggcaaaca tcctcactta cacatcccga
     3061 taactcccca accgataacc acgctgagcg atagcacctt tcaacgacgc tgatgtcaac
     3121 acatccagct ccgttaagcg tgggaaacag taagcactct gacggatagt attatcgata
     3181 aacgccggat gacacatcac ctccagcgaa cgatcacccc gatggcttgc gtcatccagc
     3241 acttgcagga acagtgcctc gctaatctct tcaccataaa acgcgctact gaatccctga
     3301 gaactgcgca gattagccgg taaatcacca gagttagata gcggctgacg gtcgatgcgc
     3361 aacgcaatcc cctcttcagc cgcaaacctg gcaacaatcg ggaaaatctg cgggaacata
     3421 tgcacatgat gatggctatc aagatgcgta ggtttgcgtc caaatagctc aatgaaacgc
     3481 aaatactgac tggcaagctc ctgagtaatt tcttccagcg gtaaagcatc ttcttctgcc
     3541 aactgccaga tccattttcc cagcacacca tcgcgggtta accccggcat agctgtcagt
     3601 ggcttgccca tagtaaggac aaagtacatc cctatggcca gactcggttc atcacgactc
     3661 aactgcaccg catggtcaat agcctgccca ttcaccagcg ccgtcgtcga tgtgacaatc
     3721 ccattgcgac aggcctcgat aatgccgtag ttctgacctt tgcttaagcc aaaatcatcg
     3781 gcattaacaa tcagtaagcg ttccataatc agcctcggtt aatgtgcttt tttaagctct
     3841 gcgatgcagt cggcaaaatt tggcagccat ttctcgtgcg ccagaatcat ctcgcgtgcc
     3901 agcagctcag catcgcgatc ggaatgcacc aacggactaa ggtttagcgc cagtaacaca
     3961 tcgttaaatt ctccgctaag tgcggcgttg ctggcagcaa tctcgaagcc tttaatggtg
     4021 tgaatcagcc ccatcacttt atcatcgaaa tgcgtaatgc gcggatgtgg cgtcgcgcca
     4081 tcgcgcccca gcttacaggt catttctacc gcccagtctg ccggaatatt atcaatatgc
     4141 ccatgatgcg ggatattaac gtaatgttca gcttgcttgt cgttgtagat agcgttgatc
     4201 acttcgcacg ctgcatcaga gtaataagcc ccaccgcgct gttccagttc tttcggctta
     4261 actttcaact ccgggttttt atacagctca aaaagttgtt tctcgacttt ctgtactacc
     4321 tgcgctcgtg cgccgccttt gtagtattcg cccatttcaa tagccagcat ctctttttgc
     4381 ttgaagtaat aaagcaaata agaacacggc aacagattca gagaacgaat taagccttcg
     4441 ctaaatggca gatcgaaaat atttttaacg ccagatgctt ttaactgccc ggatgccaca
     4501 ccatcaagca attcggcaaa gcgcgacttg ccatttacca gcacatcctt aatgaacacc
     4561 atatggttga ggccgaacaa atcgatagat aaatcatcgc tgtctttcag catcagaaca
     4621 tcgtgaataa acatcttcat gccgatcgga atattacaca cgccgataaa gcgtttaaat
     4681 ccggtatgac gataaacggc ttcagtaacc attcccgccg ggttagtgaa gttaatcacc
     4741 catgcattcg gacaaagttc ttcgacatct tttacgatgt caaaaatcac cggaatggta
     4801 cgcagacctt taaacagacc gcccgcgccg ttggtttcct ggccaagata accatgactt
     4861 aatggaatac gttcatccag ttcgcgcgcc ggtaattggc ctacgcgcag ttgggtagta
     4921 acgaaatcag catctttcaa tgcttcgcgg cgatccagcg ttttataaag cttcatcggg
     4981 acgccagcgt tatcaatcat ccgttggcag agttcaaaaa tgatatccag tttctcttta
     5041 ccatcttcga catccaccag ccataattcg ctgaccggca attcgtgata acgcttaata
     5101 aatccttcca gtaactccgg ggtatagctg ctcccgccac caatagtgac gacttttaat
     5161 ttctggctca taatttctcc cttcagtaca gaatactgat atctggcata tctgcccccc
     5221 cggacataaa taatccagca acaggacaga tatgtgaatt gtcaggtata acgacttact
     5281 gattaaattc agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa
     5341 acgttttaat aaacaaactc gggctactat aaccggcctc aaacgcaata tccgtcacgg
     5401 aatagttggt catttccagt tgttttttgg caaaattaat acggatctca ttaataatct
     5461 gcattggtgt tttgccataa tatcgttgag tcgctcgcgt caaatattcc tgtgatttgg
     5521 ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct
     5581 tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct tcgcggtaat
     5641 gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt
     5701 cgcggaaatt caatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc
     5761 ggtatacctg cgaagcgaca aagcaataag gcaacaacgg caggtaatgc tgctcaaaaa
     5821 agcgtttact gatcccaacg ttcaatatac gcgtggcacc aaactcataa aaactttgat
     5881 ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt
     5941 taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag tcgtgctgat
     6001 gcagcccgct gatactctca gttttgttat agataaacac atggaagttt ttgccattaa
     6061 acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg
     6121 actccttatg ccttcagttt ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca
     6181 agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta
     6241 ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc
     6301 atccgtgact gatccatcat ggcttttgct gcagcaaaat cgccctgctt cgcctgtttc
     6361 agtgccgcat acgccaggct gcgcgcttgt ccggagttga tgataagccc catcaccact
     6421 tcttccagct cttcagcttc cgtttgcgta tcgggaatgt tatcgagatc catcatacat
     6481 cgttcctctc tattcttacc ggcacgatta cccgtaccgg catcgattaa aatttcaggg
     6541 cgttagcgat atcttcttcg cgctcttctt tatcaatcgc gttttgtgct ttgttagcca
     6601 ccacaacaaa gggcagataa attaacgttg cgataccaag gttgaagagt gcaaccagca
     6661 atgcggcgac gctaccgttg gtgttaaaga aggctcccag accggttggc atggtccacg
     6721 gtgcaatatt ggtcaccgga ggaatgatgc ccatgtagta cgctgcgagg gtgattgccg
     6781 ccagaatcgg ttgtaccagt acaaacggga taaacatcac cgggttcatg ataattggca
     6841 gaccaaacag aatcggttcg ttaatctgga agatgccgga cggcagcgcc agttttgcca
     6901 cctgacgata atcagcacgg cgagaggcga taaagatagc caggatcagg cctaaagtcg
     6961 caccactgcc cccaaggaaa ataaaggagt ccagcatcgg cttggcccag atatggaagg
     7021 tcttaccggc tgccagcgcc gcttcgacgg aaccatattg ctgataggtc gcgatatttt
     7081 ccagcgccca cggcgtcata atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa
     7141 taccgaagaa ccagagcagt ggaacaaaga tcacataggc ccagcccacc acgctaccca
     7201 acgatgccag tggggttgag atggtatcca taatgatctg atggaagttg gtgccccagg
     7261 tattcaacgc ccaggcaata atccccatca cggaaagaat aataaagccg ggaattaatg
     7321 ccgagaagga acgcgatact gaagcaggta cgctatcggg caatttaatg acccaattgc
     7381 ggcggacaat aaaggtaaac atttctgcca ccaccaggcc aataataatc ccggagatga
     7441 tattcgctcc acctaaccag tttgcgccaa ccacataggc ctcaccgaca ctatatgggg
     7501 tgacggtcat aaatgctgca acggataaca acccagccgc cagcgcatcg actttacgct
     7561 cttctgccag cgccatgcca ataaagaacg gtgccattaa agacattatc cctaatgttc
     7621 cgttatatac gttgccgcca atacctttca ggccattaag tgtttcaatg gttgaggcgt
     7681 cgaggcgaat acctaaggaa taaaaaaacg ccccctcccc aaagcttaga aaaacgttgt
     7741 taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata aagccatttt
     7801 tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt
     7861 caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt
     7921 gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat taatttgctg
     7981 cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg actttgccat
     8041 aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg atttcgggca
     8101 acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct ttttcaccag
     8161 ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct gcctgtgcgc
     8221 gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt
     8281 tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact ccgcaagcct
     8341 taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa
     8401 aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa ttgtttctcc
     8461 ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa gataattaag
     8521 aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agctttcaga
     8581 cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg
     8641 gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca
     8701 cgttgaccca ggatgtaggt ctggcaagta ccacgagcat ggatcatgct cacttcagac
     8761 gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc
     8821 accacaggct gtacaaactg gtctttagta cgatcataag ccgtacaacc cgccagcatg
     8881 gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctctt
     8941 gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag cccgcgaacg
     9001 aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt
     9061 cgcttgtcgt ttcagtggca acgggttagc tttaaggaag ttttgtcttt tctgtctgga
     9121 ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta
     9181 atgctgaaga ggaaattcgt cgtagtatcg attttctgaa aagctacctg caaacttatc
     9241 cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggaa
     9301 agctgtgcca gatggcgatt aatgagctgc gcctggaaac cggcaacgaa tcactgcaat
     9361 ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc caggatgcca
     9421 ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcca
     9481 gcgaacaggc attgcgggaa gcagacattg aactgagcga ttttgtccgt ggcaatgaaa
     9541 aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg
     9601 tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg
     9661 gtacagacat taatccgctg tatcgtctca acaaacgtca gggtaaacag ttactggcgg
     9721 cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg gaagatgatc
     9781 gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc
     9841 tggaagggaa aaacgtacct gaacaggtcg ccagaacaat agagaactgg tatctgaaaa
     9901 ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa aagtaataat
     9961 ttgcacataa tggtttgtgc gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg
    10021 ccttattcga cctacaaaac tatgcaaatt caatatattg caggagtcga ataggcctga
    10081 caggcgtagc acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt tttaatcgct
    10141 ccccgcctgt tacactggat agataaccag cattcggagt caacagtggt acggcgttta
    10201 acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacgt
    10261 tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac
    10321 accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta
    10381 cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg gatctgtacc
    10441 gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg
    10501 tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc
    10561 gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct gtttggcctg
    10621 tttgccaata ggcgcgcagc tttgcaagca ttgcagacca tcgctgatga acaaaaactt
    10681 tgttatggcc tgctgggact ggaaccgtta agtcgcggtc gtgcatgttt tcgttcagcg
    10741 ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc
    10801 ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg tgcggtggca
    10861 ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg
    10921 ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc cgggtttgat
    10981 cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa
    11041 cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg
    11101 gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg tcggtagtga
    11161 gcaatttatc gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc
    11221 caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgtg
    11281 tcatgatgtt gaaatccatc gacatctgcc cttccgtcag tttcgctttt accacctggt
    11341 ccgctgcaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagcgg tctgcgcttt
    11401 caaggagcat ctcgccgcct tccagcaacg tcactatcct ttccatgccg ggaaacaaag
    11461 aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg
    11521 ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc atttcgccac agattcaccg
    11581 acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat
    11641 cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag
    11701 gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta
    11761 ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca
    11821 gttgcaggca ttttaccttt tg
//
LOCUS       DAZPLM010000125        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPLM010000125 DAZPLM010000000
VERSION     DAZPLM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.125"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHVC7_002843"
     CDS             complement(<1..611)
                     /locus_tag="ACHVC7_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR2116843.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHVC7_002844"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHVC7_002844"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR2116844.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHVC7_002845"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHVC7_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR2116845.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHVC7_002846"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHVC7_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR2116846.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHVC7_002847"
     CDS             complement(5085..6716)
                     /locus_tag="ACHVC7_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR2116847.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHVC7_002848"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHVC7_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR2116848.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHVC7_002849"
     CDS             7139..7411
                     /locus_tag="ACHVC7_002849"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2116849.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHVC7_002850"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHVC7_002850"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR2116850.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHVC7_002851"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHVC7_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR2116851.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHVC7_002852"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHVC7_002852"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR2116852.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHVC7_002853"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHVC7_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR2116853.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHVC7_002854"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHVC7_002854"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR2116854.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtaa aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPLM010000126        11764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPLM010000126 DAZPLM010000000
VERSION     DAZPLM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.126"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..291)
                     /locus_tag="ACHVC7_002855"
     CDS             complement(<1..291)
                     /locus_tag="ACHVC7_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR2116855.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWF"
     gene            complement(359..3810)
                     /locus_tag="ACHVC7_002856"
                     /pseudo
     CDS             complement(359..3810)
                     /locus_tag="ACHVC7_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(4154..4834)
                     /locus_tag="ACHVC7_002857"
     CDS             complement(4154..4834)
                     /locus_tag="ACHVC7_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR2116856.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(4732..5475)
                     /locus_tag="ACHVC7_002858"
     CDS             complement(4732..5475)
                     /locus_tag="ACHVC7_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2116857.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(5486..6184)
                     /locus_tag="ACHVC7_002859"
     CDS             complement(5486..6184)
                     /locus_tag="ACHVC7_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR2116858.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(6184..6513)
                     /locus_tag="ACHVC7_002860"
     CDS             complement(6184..6513)
                     /locus_tag="ACHVC7_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2116859.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(6510..9083)
                     /locus_tag="ACHVC7_002861"
     CDS             complement(6510..9083)
                     /locus_tag="ACHVC7_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2116860.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(9064..9477)
                     /locus_tag="ACHVC7_002862"
     CDS             complement(9064..9477)
                     /locus_tag="ACHVC7_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR2116861.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(9504..9935)
                     /locus_tag="ACHVC7_002863"
     CDS             complement(9504..9935)
                     /locus_tag="ACHVC7_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR2116862.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(9949..10701)
                     /locus_tag="ACHVC7_002864"
     CDS             complement(9949..10701)
                     /locus_tag="ACHVC7_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2116863.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(10709..11104)
                     /locus_tag="ACHVC7_002865"
     CDS             complement(10709..11104)
                     /locus_tag="ACHVC7_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR2116864.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(11101..11676)
                     /locus_tag="ACHVC7_002866"
     CDS             complement(11101..11676)
                     /locus_tag="ACHVC7_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2116865.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(11691..>11764)
                     /locus_tag="ACHVC7_002867"
     CDS             complement(11691..>11764)
                     /locus_tag="ACHVC7_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR2116866.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2406 a         3732 c         2879 g         2747 t
ORIGIN      
        1 gaaccagcgg ttacgcacgg aatcttcatg ccagcgggta tcgctgtagt gcgttttttg
       61 ctcatcctca gcattggcat aactgaagga cgtaatcagc cccagcgcgt ccgtaaactc
      121 ataacggtat ttcacgttaa tcccgttcag attatcgctg ccgggagcgt tcgtacgggc
      181 atgaagatac cccgcgctca gtgtggactg atgttcagac gcccatgcag gcgcaccgga
      241 tacggacaga cagatggctg cggacaaaat ggctgcacaa actttacgca taattacctc
      301 tcgcttttct gcaataaaaa aggcgccatt tctggcgccc gtatatgggt tataaaattc
      361 agctgatact gatgcctgcg gtggctttct tcatcaccac aaccagcaaa tcgctgatac
      421 ttgctgtggg ataccagtta ttcaccagcc atgctgacac cgaaaactcc agcgtcatgt
      481 ggccgcgacc agctggcata tcaataacac cactgtaaat cagcgtatta tccagcgcgg
      541 tacggttata aatttcagca ccgtttttcc gtactatcag gcggcatgac gaataaatat
      601 cgttattctc ccgctcatgt ttagcgccgc tgaatgccac cgccggaata acaatttgcc
      661 tgtcaaaagg ctgatcgtca taaatcctga cggtaatggt ccctgatggc caccgctccg
      721 gtgcccggga gtcccgcgga aaagccttac ccactgtttt gactatatcg ccttcaatct
      781 ggttggctga cagtttcccc ttaatctgac agttctcatt aattgtgaca ttgttgagcg
      841 tcccggcgtt cgcattcaca ctgccactga tatctgcatt tttagcggtc agttttccgt
      901 ccggtgtcag ggaaaatgcc ggaggattac cgccgctggt aatggtggga gccgtcaggc
      961 gtttcaggaa cacgtcgttc atgaatatct gatcgccctg accaacaaac atcggctttg
     1021 tgttgccatt cgcaggatta atcatcgcaa tcctgtctgc cgccagcagc acctgactct
     1081 gcattcctgc tggcgtattc tcaataccgg caccgatacc cgcaatataa aggcgtccgt
     1141 ccttcatctg ttgcagcttc acagcccaca tgctgttcag attattattt gtatcaacct
     1201 gaaccttctg tatctgctgg attgccgcac tctggtcttt cagtttctta ttgacagtct
     1261 gcgtgatttc attgctgaca tccgtaatgg acgtcctgat ttcagccagg tcaggcgcaa
     1321 gctgaccgtt atcaatctgc gtccacagct cctgagccag atgtgttttc cctatctctc
     1381 ctttgaaaaa atccaagtaa ccttccgcat catcgctcgc ccgaccgaca gcctccacga
     1441 atgccgattt gccaacggtg ttcacactgc gaacgtaaaa ataataatca tggcccggtt
     1501 tgatattgat actggcggct atccagtaca gcgccgtgcc aagatagcgg gcgctggttt
     1561 caacctgcct gatatccgca atccgctttt ccgagaacca gaactcaaac tgtaccgtcg
     1621 ggtcataaac ggcaagatgc ggcgttgcgg ttatctgaaa atagcccggc gtcagctcaa
     1681 tccgcgacgg tgctgccggt gcggcaatcc ggaaggtggt ggtggcaggt tcaccctgct
     1741 ggccatagct gtttatcgcc cgcaccgtca gggtgtattc cccgagcggc aggccgctga
     1801 aacggtgctc cgtgtctgcg gtgatggcgg tggtcaccag tctggcatcc gttcccttac
     1861 cactggtcag gcgcagactg aagcgcacac ccttcaccac ccgcggcgtg tcccatttcg
     1921 cctgcgccag atactggccg tcaggtgctg cactgccggt gggatgacgc tgttcaggga
     1981 acctgactgc ggctcaaagc gggcaccgtt atccacgatg gcttcttttt ccagtacgtg
     2041 ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc cggacggaga cacagcggaa
     2101 caggagacgg cgcagtgacg gcagggagag tccccacacc ccgtatgtct ccacaccatc
     2161 aggcagggta ctgacctgta tccggtccgg cgcggggtgt tcggtgatgt ccacactcac
     2221 cggcttaccg ctgccgttaa tcaggttcac cgtggcggca ccggtctccg gaagtgtcac
     2281 ttcacggtcc agcgtcagag tgcgggtggc agcatcaatg gacaggacac gtccgccggt
     2341 cagggtcccg gcatagtcgt tatcacagat ttcaatgatg tcaccgggtg tgtgccgcag
     2401 cccctgagac ccgagcgtga aatccaccgt ctgcgtttcc agcagttcgg tctttatcac
     2461 ccacagtccg gcacggtggg cctgaccgcg gctggtacag ccgaacgcgt ccatcttcag
     2521 caggttgcgt ccgtagcgca gtatggcttc cgggtcttcc accagttccg tggaggtctg
     2581 ccagccgttc tgcgggtcgg tataattcac ctccaccgcc gtgtgccggt ccttcagggc
     2641 actgaagctg tagcggaaac ccacgccgtt atcatccacc accacatcgc tgttggtgta
     2701 cggccacacc acatccgacg ggcggtcctg aacaaacgtc agcgtctggc cgttccatac
     2761 cggcatacag cgcatcgccg agcagaaatc actgagcacg tcccatgcct tacgctgttg
     2821 tgccaggtac gcattgaagg tcatccgcgg ctcggtctcc ccgaaactat ccgggaccgt
     2881 ctggtcgcag tactgcccga tggcatacag cgcccacttg tccacatccg ccgcccccag
     2941 acgttttccc atgccgtagc gcgggtgagt cagcatgtcc cacagacacc aggccggatt
     3001 gttgctgtat gccggtttca ggctaccgtc ccagatacca ctgtacgtgc gtttttccgg
     3061 gtcatagttt gacggcacct ggatgatgcg accgcggata tggtagttca ccgtcatctg
     3121 ctggccgccg aactgctccg catccacctg cagccccaca atggccgtgt tcgggtagca
     3181 ctgtttcaca tcgatgattt cggtgtatga cgaccacagc gtcttattct gcagctggtc
     3241 cgtggtgctg tccgctgtct cccggaccat ccggatgtta aaaggacggg gaggcagatt
     3301 atccagaatc accgacgcca gaaactgcga ggtggtcttg ccgttaatgg tgacatcctt
     3361 ttctgtcacc cagttaccgt tacgctgtaa ctgaatcagc aggcggacgg atgtcgggtt
     3421 tcggtcaccc tttgacgtgg tctgcaccag tgactgcacc ccgaaggtaa cccgcaggcg
     3481 gtcaatgttc gcggacgtaa tggtgcgcgt caccggtttt gccttcgtca cttccacgcc
     3541 cagtccggtt tcagcaccgg aggactcaaa gccttccggt ggtgtctgct cctgctcccc
     3601 ggcgcgtcag accgccgtca caccgtgtat cacgggatta ccgtccgtgt ccgtcagcgg
     3661 ggttttgttc accaggatac tctgcagtcc cttcaccgga ccttctatca gtccctcacc
     3721 aatcgcatca atcacgctca tcatctgcgt ggatttgaga ttatccttcg cctcacgagg
     3781 cgtgtgtgcc ttaccgccac cttttcccat acagccttcc cctgaataaa ttaaccgcca
     3841 cttgccattc cgtacagaag tcggatatcc ttcgcccgaa aagcatgaaa cacatttctg
     3901 ccatgctaaa gagaaacccc ggtatcagca gataccgggg ttttctttca tgcccaccga
     3961 taatcctgtt ggttaaaacc ggtaatggca taaaaattct gaatatcttc acattttcac
     4021 aaactgactg tggcgcgtat aatttctctg cgttaatttt tttgtcgtga tataagaata
     4081 attccttaca cttaatcttc gtaactctcc cgcagttcct gtccgcgatc actgcgggat
     4141 ttttttattc tttttacccc tgccgcccga taaccacgac ctttccgccc ccgccttcat
     4201 cacgggtgct gatgtcctgg gatatacggc gggagccaac cagcatttcc ccgtaaggca
     4261 ccggcatcgg gttcccctgg gcaatcatgt tatccagtga ggaaaagtac gtgttctgtc
     4321 tgccgttatc cgttgcgcgg taatccggtg tttttgcctt cggggccagc atctgggcca
     4381 caccgcccag aatcatgctg gctccaagtg aaaacagcat cgtggtggca gaaaaaccac
     4441 cggctgccag ggctgaaccc cataacgcca ttgatgcccc ggcagtgaag aaagagccca
     4501 cgatggctgc cgcccccagc acaatctgca gtccaccctt tccggccccg gccagtcgcg
     4561 gcacaatgtg gatgaccgtt ccctcaccca gctgttcgtg aagacgggcg tacaccgcct
     4621 ccggtgccgt gtcataaccg gcaatacgta tctggtacca gccttcgttc atctgacggc
     4681 gaaagcccgg catctgcatc gacagggcgc gaatggcttc cgctgccgtg ttcacataca
     4741 ggctgaggcg gcggccaaat cgttgtaaat ccccgtgaag gcagatgcgt gccagtggcg
     4801 gtgacgccag actgaatgcg ttcgtcgttg ccatttttcg gaatacctct cccgtttact
     4861 cagttgttca ggcagatggt gaagcagctc accgttgccg cagtaaatgg cggcatggtt
     4921 cggtaccgaa gcaccaaagc agcacagcag aatatcgccc gcctgtgcag aggacagggg
     4981 cacccggtaa aagccggtga ccgccatatt gtccaggtaa aggttctgac cgttgcgcca
     5041 ccagtcatcc tcgcgatgaa aatccggcat ttcagtcccc gccagatgat aagcatcccg
     5101 gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc cgtcctgtca gatgtggcac
     5161 acagcggaat ttgtgaatgt caccccggca gaccaaccac cagggcagtg cgctttttat
     5221 ctgcaaccgc cggtcagcct cgctcagcca gggcagccca ccgggatgac tgtggaccag
     5281 tgccacaatc tccccctgca tctctgcccg cagccagtct tccggtgcga tacgaaaata
     5341 cgcctccggc tctgcggaaa tattcacaca agggatatac cgctccccct ccggcgtgct
     5401 tatcacgaag ccgcacgact ccgcaggcgc acaccgccgg gcatgtgcca gaatcgctga
     5461 ttcagtctgt gtcataaacc gggatttact gcgaaagttt attaatggaa aggaaaccgc
     5521 caaaattgcc gacattcctg cgcagttcac acccgcgcat gcacttgctg catctgtcct
     5581 tacggatatc cgtggtgggg ttgtcgaact catccgccac cgcaggaccg ttatacccgc
     5641 attcatctcc ccggtaatcc cacatacagg tgttcgccag catgatgcga ccgggaaaca
     5701 gcgccccgtc cgtctcggtc ggtgtagcca gcacaaacga ggccgtcatg gctgtcagct
     5761 gcgacatctg ctccaccacc cagcggtcac tcagctcctg ctccgggtcc gcctccggat
     5821 tgcccgcaac gaaattcacc gcatccagaa aacgggcata cacccggcgg cggaccaccg
     5881 tggcccccac cagactctgc aggtcttccg ccatcccggt gaccagaccg aacagattgg
     5941 acaccgtcag cgacggtctg gcactgctgc cccggccgtt catctcaaag ccgctgccgt
     6001 caatcgggta tgcctcatac ttacgcccct gccaggtgac cggctcccct ttttcattca
     6061 gctcattaca gaaaaaatac cgctcaccac cctgtaccgt caggtcgatt tcccagagta
     6121 ccacccgcgg tgactgctct gacttaaccg actcgttcag actttcttcg cgaatatcct
     6181 gcatcagttc accacctgct taaactccgc gctgaactca acgcgcaaca tcccgacccg
     6241 cgcagaccac ccggcacagg tcacctttat ctgccggtat gcatagggtg gcttccacaa
     6301 aaatgccttc cagccaccgt gctctgccag gaacgcttcc agatgccggg cctcctcccg
     6361 ggtcacggaa agcgtcaccc tgtatgtttt caggtcagca ttcagccctg ccgccatacg
     6421 ctgtgagtac ccgtcaccaa aacgcacttc acgcaccgat ggctgcgagt tcacctccat
     6481 atccggcttc actttccagc gaaaggtttt catccaccgc tccctgataa cataccgcca
     6541 tcacgcaact gcagccggag ttcatcctgt gcccccttgc gggccatctc atacaccgct
     6601 ttcatcagct gcggccctgc ccgcccgttg gggccgtcgt tctgaatcac cacgtgattg
     6661 ttctgattaa aattaatgcc ttccgcccgc cgcatctgcg ccggacttcc ggcaccgccg
     6721 acataaccac cttccgcata gccccgcatc aggcggtaca gattgccgac accaatccgg
     6781 ctggtcgcct ccttcgtgaa gacaaactcc ccgcgatgaa caatccccgc aggttcatat
     6841 ttaccccccg tccccgtaaa tcccccggtc gcgaaatgga agttcgccgc cgcagcctga
     6901 atggccgtcc ccgtggaggc agacgcacca ccaccgaaag caccgccaat ggcgctgccg
     6961 atactcccga ctatccccac catcgcctgc ttcagaaaaa tctctgtcag catggacagc
     7021 acagaacggg tgaaaccacg ccagttctgt tcgctgccga tcagcatcgc tgccatattc
     7081 tgtgcaatac cgtcaaaggt ctgcgtggcc gcgtttttaa cctgcgaaaa actgtccgtc
     7141 gcactttccg cccactcgcc ccagccggac ttcatcccgg ccatccagct tccacgaagc
     7201 tgctcctccg cagaccaggt gttcttcagt gcagatgtgg ccttcgccag cgcagccgga
     7261 ttatcaccgt acacctcacg aaggcgctgc tcttccgact cccgctgcgc ctgacggtcg
     7321 gtgagtccgc gggcttttgc gctgattgcc gcctgcttcg cgctctgctg ctgttcaaac
     7381 cgcgccgcct gctgtgccag ctcattcagc cgtttctggt gttcaatctt gtcgcccagc
     7441 tcagccagct ggcgtttgta ctccagcgtt tctttctcat gggccagcag ggatttttcc
     7501 tgctcagata actgccgttt cgtggcggcc tctttcagga ccacatactg attttccgct
     7561 ttccataaat cacggcgctg ctggctgatt ttttcattcg caccgctgtg cttctccagc
     7621 gtcctgagct cagtttcaag cgccagcagg gctgcatgcg cctggtcttc ctggcgctca
     7681 ccggcagaca ccttcacacc ggacggcttt ttctgcgtcg actcataatc cttttttgcc
     7741 gacgccatca gcgtgttgta atccgcctgc aggattttcc cgtctttcag ggccttattc
     7801 agctcttcct gccgggcggt atatttctcc agtggcgtca gcaggcgctc atacgccttc
     7861 tgcgcctctc cggtatactt cagctgtgac gactcacgct cagccctgtc ccttgccgcc
     7921 agttcaccgg ctttttccat atccgactgc agcgttgccg ctgccagacc cagacgggca
     7981 ttttcccggt catcccatgc gccctgaagg ttggcccgga aagaggaggt tttaccgcgg
     8041 cgctggctcc gactctggta ccactgccat tttttatccg cctcatcaaa tgccttctgc
     8101 gcactggcga gcatatccgc tgaggattcc ggacgaccga tatccagaat ggcatcccac
     8161 atcgatttga atgccttccc tgttttatcc gcccaggtct ccagtgttcc catgttttct
     8221 ttcaggcgac gggtctgctc atcaaagcct ttcgtggcga tatcgttcgc cgcctgcaat
     8281 gccccggcct cgtctccgga acgctgcagc tgtgcaacat acgcaatctg ctctgccgtc
     8341 acgttacgga actggcgcgc catcgccatc agtcccgacg tcgggtcagt ggtcagcttc
     8401 ccgaaggctt cagcgacttt atccacctcc acaccggatg cagacgcaaa acgcgcgaca
     8461 ctctggttga tggcatcaaa ctgttcacca ccacgcacac cggcattcac cagggctgcc
     8521 agtgactctc tcgcctggtt aaacgtcagc cctgctgcct gcccggctct tgagagagtc
     8581 agcatacgat cggcagtcag tccggactga ttaccggaaa gaaccagggt tttattaaac
     8641 gctgaaagcg tggaatctcc ctggtaccag gcgtacacca gtgcacctgt cgccaccgcc
     8701 agcgaggtga ccccgaccat cggcagggtg atcgcatcgg caagccccct gaacatgggg
     8761 atcatcccgc cgaaggagtc cttcacctga ccgccctgtt gcagcaggat cagccaggga
     8821 ttctgaccac cggcaagctg cgtggcgata tccgtaaact gtgcgggcag ggttcgcatg
     8881 gccgctttat actgcccgac ggaaatcccg gctttttgtg cagccagcgc ctggcggctc
     8941 aggccctgtt caacagcact ggcggttttt ctggcgtcgg tatccagacc tgaaaaatga
     9001 cgccttaccc ggctcatctg ctcatcgaaa cggacagcat ccagactcag gtcaataaca
     9061 agatcaccaa ccggctggga catatctcac acctcccgga atccccgctg aagccatcat
     9121 taatgcggca tcatccacca tgacatccgc cacatccgca gacgataaaa tatcgcgccc
     9181 tccgtcccca ccgaaccgga cgcctccggc aagtcctgcc gctttctgca tcagcatttt
     9241 gtcctcatcc ggcctctcca cctgctcttc ctcatgccgg gggacaagca gactgaaatc
     9301 agagggatgc atatccggat cgcaaaaaaa caggctgagt acagcgtacg tcagcccgga
     9361 aaaatgcata tccagctggg tatcctgaaa ataatgcgtg cggtaaaaac ggcgccagtc
     9421 ggcatattcg gtggatgtca tcccggcaag catggcgcgc cagtcgggtc tccccatctc
     9481 acgcgccagt ctgagggcaa agttcagctc gccgtcgaag actttcccgc agaaaaatca
     9541 tcatcagtca gcgtgttatt tttcgccact tcagtaatat cagtatccgg acgaacagct
     9601 tcgatcatcc cggacaggca caacaccacg tcttccgccc gggcaatggc atcggcaggc
     9661 caggtggtga gcacttcctg ctctatcttc atcacggcct cattcattga cggtgactgc
     9721 gttttctgtg gatggttatg ccacagggac atcgccacca gaaacgcgcc ggttctgacg
     9781 agatcttcca cgcttacctg caggttgccg ctggattctg cctgttctgc acgccgtttc
     9841 aggagggcaa gatgctcgat acgctgcagc gcagacaatt cggaaagcgt gacagacaca
     9901 ccgttatatt caaattgttc tgttttcaga aacatgtatt acctccgttt accctgcagc
     9961 gcccgcttca gtaacggtga cttcagccac tgcggcgaac tgaccatttc cgctcaccac
    10021 agggatctgc accttacctg tcgccacgcc gtttaccgta attgtcatat ctttcacact
    10081 aatggtggct ttcgacggat cggcggaaac cgctctgaac gtcttgtcgg ttgcactttc
    10141 cggctcaaaa gaaaccgtca gggtggttgt tttccctttt gccaccgtac cggatgtcgg
    10201 cgtcacctta atcgcactga ccggcgtaat tttgctgcgt tcttccgcta cagaaggttt
    10261 acccacgtta gtgactttca ccgtgcgggt gatcacttct ttcgccgtca cggccttacc
    10321 gatactgctg acccagccac gaaacacatc caccgtgcca ttcggaaaac ggattttata
    10381 ggcccggaca tcgccgcttt caaaccagcc tataagccct ttctggcctt cctctcccgg
    10441 tttccaggcc agcgtaaaac tggtatcacc tgcagatttc tgcccctgcc cggtcgcgct
    10501 ccagtccgcg tcttcatcat ccaggtagtt atcatcatag gattcagccg tcatctcgcc
    10561 cggcgtcaga tccttcacct tagccagtcg ctgccagtca tcgtctgaca acgggtttgc
    10621 ataagcatca gccttgccgt tgtaaaccca cagagtggta ccggcacctt ttaccggctc
    10681 caggggattt ggtgttgcca tatcgtcctc acatctcgta tgtaatggaa taagtcagat
    10741 ccgcagagct ccataacgcc atatcgtcat cacgacgata ctcatagccc tgcgtaacca
    10801 tcgtggtaat cagtcctgcc agtgcaggga tcgcagtcat cgccggataa atccggcttt
    10861 ccatccactg atcgagctcc gaatcaggta cctgtgccgg taaaaacacc tcaatatgca
    10921 gtgtggcccg ccaggtatct gcatccagct cttcaccggt atactctgca tccgtcagat
    10981 aaaccgcgat cgcaggaaaa tcctcttcgt caaaaacaac ggggcgacca tcaaacagcg
    11041 tcgccccgtg ttcatgctgc tcgagtgcat ccagcactgc ggcacgaatg tcagtgtgtt
    11101 tcatcgtttt atcgcaatcc tcagttgttg tttcagcgcg tatgccagtt ctttaggcag
    11161 gcgttcacgc cggatacggt caacattctc atcaaatgcc tgtttcagtg gggccgccat
    11221 cgggattttc accacctgaa tgggaaggcg attacgcttt ttccttccct tgtcgtcatt
    11281 gccctcctca tatctggcct ggggaagacg ttgcataaca tgccagcgcc cattatttaa
    11341 tcgctggata aatgcccgct gataacgatg ctgaccggct ttgagtatgc tgttcggacg
    11401 acgccccagc attctgatcc ccagcttaat cacagggaga tcaccgcggt taacgataat
    11461 tctggcattc ggatttctga ccgtcgcccg tttcagtctg gaccgttcct ttaccagttt
    11521 ccgtctcacc ctggtttccc gggcaacctg tgacgaagac tgattaatcg ccgttgtggc
    11581 cacgcggtta atggtcattg cagaagcagc cggaatggcg tttttacgaa cccggttcag
    11641 attgtcaatc gcctgatcaa gcccttttat cgccataatt caccctgcgt ttatcgtcgc
    11701 cggttaactg cgggtggttg cccacggttg agccagagat aacagctgcc cccgtcatcc
    11761 ggag
//
LOCUS       DAZPLM010000127        11749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPLM010000127 DAZPLM010000000
VERSION     DAZPLM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.127"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..544
                     /locus_tag="ACHVC7_002868"
     CDS             <1..544
                     /locus_tag="ACHVC7_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2116867.1"
                     /translation="AALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDIDKE
                     VELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFDSSAKTTGA
                     DPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAWMEQIDQ
                     ATPVTPGAVTANATYVLDYK"
     gene            700..2732
                     /locus_tag="ACHVC7_002869"
                     /pseudo
     CDS             700..2732
                     /locus_tag="ACHVC7_002869"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2709..3053
                     /locus_tag="ACHVC7_002870"
     CDS             2709..3053
                     /locus_tag="ACHVC7_002870"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR2116868.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3068..3799
                     /locus_tag="ACHVC7_002871"
     CDS             3068..3799
                     /locus_tag="ACHVC7_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2116869.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3796..4857
                     /locus_tag="ACHVC7_002872"
     CDS             3796..4857
                     /locus_tag="ACHVC7_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2116870.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5009..6055
                     /locus_tag="ACHVC7_002873"
     CDS             5009..6055
                     /locus_tag="ACHVC7_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR2116871.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6052..6843)
                     /gene="nei"
                     /locus_tag="ACHVC7_002874"
     CDS             complement(6052..6843)
                     /gene="nei"
                     /locus_tag="ACHVC7_002874"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR2116872.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6879..7613)
                     /gene="pxpA"
                     /locus_tag="ACHVC7_002875"
     CDS             complement(6879..7613)
                     /gene="pxpA"
                     /locus_tag="ACHVC7_002875"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR2116873.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7603..8535)
                     /gene="pxpC"
                     /locus_tag="ACHVC7_002876"
     CDS             complement(7603..8535)
                     /gene="pxpC"
                     /locus_tag="ACHVC7_002876"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR2116874.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8529..9185)
                     /gene="pxpB"
                     /locus_tag="ACHVC7_002877"
     CDS             complement(8529..9185)
                     /gene="pxpB"
                     /locus_tag="ACHVC7_002877"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR2116875.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9208..9951)
                     /gene="ybgI"
                     /locus_tag="ACHVC7_002878"
     CDS             complement(9208..9951)
                     /gene="ybgI"
                     /locus_tag="ACHVC7_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR2116876.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10222..11703
                     /gene="dtpD"
                     /locus_tag="ACHVC7_002879"
     CDS             10222..11703
                     /gene="dtpD"
                     /locus_tag="ACHVC7_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR2116877.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2800 a         3150 c         2889 g         2910 t
ORIGIN      
        1 ggccgcactg gccgtatctc tgctgttcac agcacctgtt tatgcggctg atgaaggatc
       61 cggtgaaatt cactttaaag gtgaagttat tgaagcaccg tgtgaaatac atcaggatga
      121 tattgataaa gaggttgaac tcggtcaggt gaccaccagc cacattaatc agtcacatca
      181 cagcgatgcc gttgctgtcg acctgcgctt agtcaactgt gatctggaaa actccagcaa
      241 cggttccggt ggcaagattt ctaaagttgc agtcaccttt gatagctcag cgaaaaccac
      301 cggcgcagat ccgattctca acaacaccag cacgggtgaa gccaccggcg tcggcgtacg
      361 tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc gctactccag atatcgacct
      421 ggctccgacc tccagcgaac aaacgctgaa tttctttgcc tggatggaac aaattgatca
      481 ggctacacct gtaacgccag gcgcagttac ggcgaatgca acctacgtgc tggattataa
      541 ataagtatta ctttggcggc gctatcgccg cctttcataa aatatttatc aggtatggac
      601 accgtgaata tttatcgact ctcttttgta tcctgcctgg tcgtggcgat gccttgcgca
      661 ttggcggtcg aattcaacct taatgttctc gataaatcga tgcgcgaccg cattgatatt
      721 tcattattaa aggaaaaagg agtcattgct cccggtgagt attttgttag cgttgcggta
      781 aataataacc aaatcagtaa cgggcaaaag attaactggc acaaaaatga cgataaaacc
      841 attccgtgca tcaatgattt actggtcgat aaatttggct taaaacctga agtccgtcag
      901 tcgttaccat tgataaatca gtgcgtcgat tttagctccc gacctgaaat gctcttcaat
      961 ttcgatcaag ccaatcagca actgaatatt agtattccgc aagcctggct ggcgtggcac
     1021 tcagaaaact ggcccccacc ctccacatgg aaagaaggtg tcgccggtat cctgatggat
     1081 tacaacttgt ttgccagcag ctaccgccca caggacggca gcagcagcac taacctgaac
     1141 gcctacggta ccaccggaat taacgccggg gcatggcgct tacgtagtga ttatcagttg
     1201 aatcagactg atagcgatga taaccatgaa cagtcaggcg gaatatcgcg cacctatctt
     1261 tttcgtccat taccgcaatt aggctctaaa ttaaccctcg gcgaaacgga ttttagttcc
     1321 aatattttcg acggtttttc ttataccggc gcggcactgg caagtgacga gcgaatgttg
     1381 ccatgggaac tacgcggcta cgccccacaa attagcggta ttgcacagac caatgccacg
     1441 gtgacgatca gtcaatcagg ccgcgtcatt taccagaaaa aagtcccacc aggcccattt
     1501 atcattgacg accttaatca gtctgttcag ggcacactgg atgtcaaagt gacggaagaa
     1561 gatggtcggg tgaacaattt ccaggtttcg gcagcatcga cgcccttcct gactcgtcag
     1621 ggacaggttc gctataaact ggccgcgggt cagccacggc cctccatgtc acatcaaact
     1681 gaaaatgaaa ccttttttag caatgaagtt tcctggggga tgctgtcaaa cacctcgctg
     1741 tacggcggcc tgctgctttc tggtgatgac taccattctg ccgcaatggg tattgggcaa
     1801 aatatgctgt ggcttggtgc gctgtcgttt gatgtcacgt gggccagtag ccattttgat
     1861 actcagcagg acgagcgggg cttaagctac cgttttaatt acagcaaaca agtggatgcc
     1921 actaacagca cgatttccct cgccgcttat cgtttctccg atcgtcattt tcacagctac
     1981 gccaactatc tggatcacaa atacaacgac agcgatgcgc aggacgaaaa acagacgatc
     2041 agcttattgt gggccaaccg attaccccac taaacctcaa tctttacgcc aacctgctac
     2101 atcaaacctg gtggaatgca gacgcctcca cgaccgccaa catcacggca ggttttaatg
     2161 ttgatattgg tgactggaga gatatctcta tttcgacgtc attcaataca acccattacg
     2221 aagataaaga tcacgacaac cagatttacc tgtcgatttc gctccccttc ggtaacggtg
     2281 gtcgggttgg ctatgacatg caaaacagta gccacagcac cacacaccgc atgtcgtgga
     2341 acgatacgct ggatgaacgt aatagctggg gcatgtctgc cggactgcaa tccgaccgtc
     2401 ctgacaatgg agcccaggtg agcggtaact atcagcacct gagttcagcg ggtgagtggg
     2461 atatttctgg tacctatgcc gccaatgatt acagttccgt cagcagcagc tggagcggtt
     2521 ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac
     2581 gcctgatggt cagcaccgat ggcgtggcag atattccggt tcagggcaat ctcgactaca
     2641 ccaaccattt tggcattgcg gtggtgccgt tgatttccag ttaccagcct tccaccgtgg
     2701 cggtgaacat gaatgactta cccgacggcg taacagttgc agaaaacgtt atcaaagaaa
     2761 cgtggattga aggcgcgata ggttacaaat cactggcttc ccgttccggt aaagacgtta
     2821 acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct cggagcggat atccgccagg
     2881 atgacagcgg cattagcgtg gggatggttg gcgaggaagg acatgcctgg ttaagcggag
     2941 tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag ccagcattgc tcgctccatc
     3001 ttcctgaaca tatggaagac accgcaaatc gcctgatttt accttgtcat taattaagga
     3061 aatgaaaatg acatttatga aaggactacc ccttttgttg ttggttgcca gtctgtgcag
     3121 ccacgctgca ctacaacccg atcgcactcg tattgtgttt aacgctaatg ataaagctac
     3181 cagtctgcgg gtggataatc gtagcgataa actcccctac ctcgcttatt cctggctcga
     3241 aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga tacaacgtct
     3301 ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa gcctcgacca caaaattacc
     3361 gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa
     3421 aaacagcgac catgccgttc tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg
     3481 accggctgca ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca
     3541 gcagggcaac caactgacac tgaaaaatcc taccgcgtat tatctgacca ttgcctacct
     3601 gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg cgccttttag
     3661 tacggtcaac acaaacaccg gaaattatag cggcagccag ttttatctcg gttatatgga
     3721 tgattacggc gcgttacgca tgacgacgct caactgcagc ggacaatgcc atttacaagc
     3781 cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg tctgttattt
     3841 ctgccgttga agtctgcgct tgccctgaac tgctactttg gttcatccgg tggctcggta
     3901 gaaaaatcag aagcgattca accttttgct gtaccaggca atgccaaact cggcgataag
     3961 atttgggaat ctgacgatat taaaattccc gtctattgtg acaacaatac caacggcaat
     4021 tttgaaagtg agcatgttta tgcctgggta accccctacc cgggggtgca ggatcgctat
     4081 tatcaactcg gcgtaactta caatggcgtt gattatgacg ccaaccaggg aaaaagccgc
     4141 atcgacacta accagtgtat tgacagtaag aacattgaca tttatacccc tgagcagatt
     4201 atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg
     4261 cgtacctttc tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc gccgcatgat
     4321 tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac
     4381 gaagacccta ctgcccaaaa cctgaaatat catattactg gtctggaaaa cattcgcgtg
     4441 ctggattgca gcgtcaattt ttccatttca ccggaaacac aggtgattga ttttggtaaa
     4501 tttaatttgc tggatattcg tcgccacaca atgtcgaaaa cattcagtat taagacgaca
     4561 aaatcacaaa atgaccaatg caccgacgga tttaaagtca gttcctcctt ttataccgaa
     4621 gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg
     4681 ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc
     4741 agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc
     4801 cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt
     4861 gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcccc
     4921 ttttccttga ttgctgttat tttttcctgg cccggctttc tttatgctgt atcgatacac
     4981 caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt
     5041 gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact
     5101 tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg
     5161 ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg
     5221 ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac
     5281 gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa
     5341 taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga
     5401 ttacggcgca gatattcgcc tggttgcatt tatgcaatat ctgcgagtgc tgttcgtcgc
     5461 gggggccgct gttctggtca cccgcatgat gctgggtgat aacgctgaag cggttaacca
     5521 gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt
     5581 cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat
     5641 gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct
     5701 gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat
     5761 cttactgcgg gcactacgcc cgctaccgca aatcctgctg tcgatttttg ctctgctggc
     5821 tatttgtgcg ggtatggcgt gggggctgac ccggtttatg cacattgatt ttatgactgc
     5881 ctacctcgcc accagtcccg gcgggcttga taccgtggcg gtcatcgccg cagggagcaa
     5941 tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac
     6001 ggggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg
     6061 gcagccaggg caccagtaaa acgggcgaga tgacagcgtg gttttctcaa tgatgctgcc
     6121 acagcgttcg cacggttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg
     6181 atgcttattc tcatccacct gccccctcgt agcgtaggaa aatcgaggaa tatccagtaa
     6241 cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt
     6301 caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc
     6361 ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc
     6421 ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa
     6481 cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag
     6541 aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc
     6601 gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc
     6661 gttggaaaaa tgagttaaca acgccttacc acgcgtttcc acatgggtaa cgtgttgacc
     6721 gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg
     6781 tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg
     6841 catggtgata tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc
     6901 ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca
     6961 tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg
     7021 ccgtgttgca ccatctctag cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg
     7081 cctcgctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact
     7141 tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc
     7201 ccaacgagaa tcaatgccgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt
     7261 tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg
     7321 ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa
     7381 acggtttctg gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc
     7441 gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatgatt
     7501 tgcgcatcgc ctgcatgaaa tccacaggca atattggcag aggaaaccag cgttaatagc
     7561 tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag
     7621 ccgccacact aattgttcga aataacgttg ctgatcttgc cgcgctttca gtgcctcttc
     7681 cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat
     7741 atcagcctca atgatacagg caatacgcgg gtaaccatcg gtggtctgtg cgtcgttcat
     7801 caacacaatc ggctgcccgt tatgtggcac ctgcaccacg cccggtaaca aaccgtgaga
     7861 taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg
     7921 gttactttgc gagctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc
     7981 gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt
     8041 aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc
     8101 cttcagtaaa cggccttcca gcccgccaat ccccactttg agatcggtgc tgcatgaccc
     8161 cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca tcccgtgctg
     8221 cgggcgttta agcgttaaac gctggcctgc tttcatcggc aatcgccagc cggtccagac
     8281 ggcattatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt
     8341 ttcgaactca accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt
     8401 acccaccagc aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat
     8461 tcccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca tgcccgcacg
     8521 aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt
     8581 aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac
     8641 tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca
     8701 gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc
     8761 aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg
     8821 gaggagtgca attcaacaac ctgtttttcg ctaaacccgc aatgcgccgc gaccaccgcc
     8881 aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt
     8941 tccagggcct cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc
     9001 gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca
     9061 ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc
     9121 accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt
     9181 tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac
     9241 atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac cacgttcagt
     9301 ggcatggtga cctgcagcat aaaaatgcaa cccctgctcg cgggctgaat gaatggtctg
     9361 ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa
     9421 actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc
     9481 acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg
     9541 cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat
     9601 ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtacgt caagcggcaa
     9661 atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat
     9721 tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc
     9781 cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat
     9841 tttttgcacc gtctctttgc cttctacctg caaaccattc ggcgcgtaat cactaatcgc
     9901 cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc
     9961 ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc
    10021 aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat
    10081 cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc
    10141 tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt
    10201 gcagtcaatt gtggaattat tatgaataaa cacgcatcac aaccgcgcac tatttactat
    10261 gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt
    10321 ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc
    10381 tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc
    10441 ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg
    10501 ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgattat cgtttgtggc
    10561 tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat
    10621 ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc
    10681 gcacctatcg cctgtggtta cgcccaggaa gagtacagct gggcgatggg ctttggcctg
    10741 gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcaca
    10801 catacccgcg gcgttaataa aaaagtactg cgtgcaacaa actttctcct gcctaactgg
    10861 ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtact gttctggaaa
    10921 gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa
    10981 atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat tgtgacgctc
    11041 accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc aattagcctt
    11101 tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac tgctatgttc
    11161 cagtcgatta atgccttcgc agttatgcta tgcggtgtgt tcctggcgtg ggtggttaaa
    11221 gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct tggccttggc
    11281 ctgatgagcg ccggattctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt
    11341 cactcttctc tgccactgat ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt
    11401 atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta
    11461 ttaaccggca tctacatgct gctttctggc gcgatcgcga actatctggc gggcgtgatt
    11521 gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat
    11581 gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta
    11641 ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca
    11701 taataggcca gcaccgatgc ctgatgcgac gcttgacgcg tcttatcag
//
LOCUS       DAZPLM010000128        11631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPLM010000128 DAZPLM010000000
VERSION     DAZPLM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.128"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            577..2145
                     /gene="cydA"
                     /locus_tag="ACHVC7_002880"
     CDS             577..2145
                     /gene="cydA"
                     /locus_tag="ACHVC7_002880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR2116878.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2161..3300
                     /gene="cydB"
                     /locus_tag="ACHVC7_002881"
     CDS             2161..3300
                     /gene="cydB"
                     /locus_tag="ACHVC7_002881"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR2116879.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3315..3428
                     /gene="cydX"
                     /locus_tag="ACHVC7_002882"
     CDS             3315..3428
                     /gene="cydX"
                     /locus_tag="ACHVC7_002882"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR2116880.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3428..3721
                     /gene="ybgE"
                     /locus_tag="ACHVC7_002883"
     CDS             3428..3721
                     /gene="ybgE"
                     /locus_tag="ACHVC7_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR2116881.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3871..4275
                     /gene="ybgC"
                     /locus_tag="ACHVC7_002884"
     CDS             3871..4275
                     /gene="ybgC"
                     /locus_tag="ACHVC7_002884"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR2116882.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4272..4964
                     /gene="tolQ"
                     /locus_tag="ACHVC7_002885"
     CDS             4272..4964
                     /gene="tolQ"
                     /locus_tag="ACHVC7_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR2116883.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4968..5396
                     /gene="tolR"
                     /locus_tag="ACHVC7_002886"
     CDS             4968..5396
                     /gene="tolR"
                     /locus_tag="ACHVC7_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR2116884.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5461..6693
                     /gene="tolA"
                     /locus_tag="ACHVC7_002887"
     CDS             5461..6693
                     /gene="tolA"
                     /locus_tag="ACHVC7_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR2116885.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6826..8118
                     /gene="tolB"
                     /locus_tag="ACHVC7_002888"
     CDS             6826..8118
                     /gene="tolB"
                     /locus_tag="ACHVC7_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR2116886.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8153..8674
                     /gene="pal"
                     /locus_tag="ACHVC7_002889"
     CDS             8153..8674
                     /gene="pal"
                     /locus_tag="ACHVC7_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR2116887.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8684..9475
                     /gene="cpoB"
                     /locus_tag="ACHVC7_002890"
     CDS             8684..9475
                     /gene="cpoB"
                     /locus_tag="ACHVC7_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR2116888.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9640..9715
                     /locus_tag="ACHVC7_002891"
     tRNA            9640..9715
                     /locus_tag="ACHVC7_002891"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9673..9675,aa:Lys,seq:ttt)
     gene            9865..9940
                     /locus_tag="ACHVC7_002892"
     tRNA            9865..9940
                     /locus_tag="ACHVC7_002892"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9898..9900,aa:Val,seq:tac)
     gene            9944..10019
                     /locus_tag="ACHVC7_002893"
     tRNA            9944..10019
                     /locus_tag="ACHVC7_002893"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9977..9979,aa:Lys,seq:ttt)
     gene            10166..10241
                     /locus_tag="ACHVC7_002894"
     tRNA            10166..10241
                     /locus_tag="ACHVC7_002894"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10199..10201,aa:Lys,seq:ttt)
     gene            10275..10350
                     /locus_tag="ACHVC7_002895"
     tRNA            10275..10350
                     /locus_tag="ACHVC7_002895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10308..10310,aa:Lys,seq:ttt)
     gene            10375..10506
                     /locus_tag="ACHVC7_002896"
     ncRNA           10375..10506
                     /ncRNA_class="other"
                     /locus_tag="ACHVC7_002896"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10755..>11631
                     /gene="nadA"
                     /locus_tag="ACHVC7_002897"
     CDS             10755..>11631
                     /gene="nadA"
                     /locus_tag="ACHVC7_002897"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR2116889.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2893 a         2780 c         3089 g         2869 t
ORIGIN      
        1 taatatcggc ggggtggatt tatgccttta ttagtaatcc tgaaactctg cgtcgtatta
       61 gccagtgacc aaaaaaagaa ttaaggtcaa ccgtgctgtt tttgcttcgt ctctttttat
      121 ctttaaattg ccaaccgaaa ctaatttcag ccttataact cacacatttt aaacataaat
      181 gtcactaaag ttaccttatt gaaacatgat taacataatt tgtaggaatt gatatttatc
      241 aatgtataag tcttggaaat tggcatcaaa aagagataaa ttgttctcga tcaaattggc
      301 tgaaaggcgg taatttagct ataaattgat caccgtcgaa aaatgcaaat ttgcttcaac
      361 aaaaacctgt ttattgtaag gattttgcgg cgtaatatat acgtgggatc aatttgagtt
      421 tttattaaca tgtttgcaac ctttctttac gccgtttttg tgtgcattca catggtatga
      481 tgaaagtgtt caaacaaatt tctattgggg catgcgtgtg accctttcta acggggttca
      541 ctctcggagt cttcatgcga tgagcaagga gtcatgatgt tagatatagt cgaactgtcg
      601 cgcttacagt ttgccttgac cgcgatgtac cacttccttt ttgtgccact gacgctcggt
      661 atggcgttcc tgctggccat tatggaaacg gtctacgtcc tctccggcaa acagatttat
      721 aaagatatga ccaagttctg gggcaagttg tttggtatca acttcgctct gggtgtggct
      781 accggtctga ccatggagtt ccagttcggg actaactggt cttactattc ccactatgta
      841 ggggatatct tcggtgcgcc gctggcaatc gaaggtctga tggccttctt cctcgaatcc
      901 acctttgtag gtctgttctt cttcggttgg gatcgtctgg gtaaagttca gcatatgtgt
      961 gtcacctggc tggtggcgct cggttcaaac ctgtccgcac tgtggattct ggttgcgaac
     1021 ggctggatgc aaaacccaat cgcgtccgat ttcaactttg aaactatgcg tatggagatg
     1081 gtgagcttct ccgagctggt gcttaacccg gttgctcagg tgaaattcgt tcacactgta
     1141 gcgtctggtt atgtgactgg cgcgatgttc atcctcggta tcagcgcatg gtatatgctg
     1201 aaaggtcgtg acttcgcctt cgctaaacgc tcctttgcta tcgctgccag cttcggtatg
     1261 gctgctgttc tgtctgttat tgttctgggt gatgaatccg gctacgaaat gggcgacgtg
     1321 cagaaaacca aactggctgc tattgaagcc gagtgggaaa cgcaacctgc gcctgctgcc
     1381 tttactctgt tcggcattcc tgatcaggaa gaggagacga acaaatttgc gattcagatc
     1441 ccttacgcac tgggcatcat tgcaacgcgt tccgtggata ccccggttat cggcctgaaa
     1501 gagctgatgg tgcagcatga agaacgcatt cgtaacggga tgaaggcgta ctctctgctc
     1561 gaacaactgc gttctggttc taccgaccag gcggttcgtg accagttcaa tagcatgaag
     1621 aaagacctcg gttacggtct gctgctgaaa cgctatacgc caaacgtggc tgatgcgact
     1681 gaagcgcaga ttcaacaggc aaccaaagac tccatcccgc gtgtagcgcc gctgtacttt
     1741 gcgttccgta tcatggtggc gtgtggcttc ctgcttctgg caatcatcgc gctctctttc
     1801 tggagtgtca tccgcaaccg cattggcgag aaaaaatggc ttctgcgcgc cgcgctgtac
     1861 ggtattccgc tgccgtggat tgctgtagaa gcgggctggt tcgtggctga atatggccgc
     1921 caaccgtggg ctatcggtga agtgctgccg acagctgtgg cgaactcgtc actgaccgca
     1981 ggcgatctca tcttctcaat ggtgctgatt tgcggcctgt ataccctgtt cctggtggca
     2041 gaattgttct taatgttcaa gtttgcacgc ctcggcccaa gcagcctgaa aaccggtcgc
     2101 tatcactttg agcagtcttc cacgactact cagccggcac gctaagacag gagtcgtcaa
     2161 atgatcgatt atgaagtatt gcgttttatc tggtggctgc tggttggcgt tctgctgatt
     2221 ggttttgcag tcactgacgg tttcgacatg ggggtgggca tgctcacccg tttcctcggt
     2281 cgtaacgaca ccgagcgtcg aattatgatt aactccattg caccacactg ggacggtaac
     2341 caggtttggc tgatcaccgc gggcggcgca ctctttgctg cctggccgat ggtctatgcc
     2401 gctgcgttct ccggcttcta tgtggcgatg atcctcgtgc tggcgtcttt gttcttccgt
     2461 ccggtcggtt ttgactaccg ctccaagatt gaagaaaccc gctggcgtaa catgtgggac
     2521 tggggcatct tcattggtag tttcgttccg ccgctggtaa ttggtgtagc gttcggtaac
     2581 ctgttgcagg gcgtaccgtt caacgttgat gaatatctgc gtctgtacta caccggtaac
     2641 ttcttccagt tgcttaaccc gttcggcctg ctggcaggcg tggtgagcgt agggatgatc
     2701 attactcagg gcgcaaccta tctgcaaatg cgtaccgtgg gcgaactgca cctgcgtacc
     2761 cgtgcaacgg ctcaggtggc tgcgctggtg acactggtct gtttcgcact ggctggcgta
     2821 tgggtgatgt acggtatcga tggttatgtc gtgaaatcga caatggacca ttacgcagcc
     2881 tctaacccac tgaataaaga agtggttcgt gaagctggcg catggctggt taacttcaac
     2941 aacacgccaa ttctgtgggc tattccggca ctgggtgtgg ttcttccgct gctgaccatc
     3001 ctgactgcac gtatggataa agccgcgtgg gcgtttgtgt tctcctccct gacgctggcc
     3061 tgcatcatcc tgacagccgg tatcgcaatg ttcccgtttg tgatgccgtc cagcaccatg
     3121 atgaacgcaa gtctgacaat gtgggatgca acttccagcc agctgacgct taacgtcatg
     3181 acctgggttg cggtggttct ggtaccgatc attctgctct acaccgcttg gtgttactgg
     3241 aaaatgttcg gtcgtatcac caaagaagat attgaacgta acacccactc tctgtactaa
     3301 gtaaggagct aaaaatgtgg tatttcgcat ggattctggg aacgcttctt gcctgttcgt
     3361 ttggggtaat caccgcgctg gcgcttgaac acgtcgaatc aggcaaagcc ggtcaagaag
     3421 acatctgatg agtaagatta tcgcgacctt gtatgcggta atggacaagc gccccctgcg
     3481 ggcgctttcc ttcgtgatgg cgcttctgtt agcaggatgt atgttttggg acccatctcg
     3541 ttttgccgcg aagaccagtg aactggaaat ctggcatggt ttattgctga tgtgggccgt
     3601 ctgtgctggt gtgattcacg gcgtgggctt tcgtccgcag aaggttcttt ggcaagggat
     3661 tttttgccca ttgcttgccg atattgttct cattgtcggg ctgattttct tcttctttta
     3721 aatcagaatt ctctttaaaa aattatgggc cgctccaggc ccataaattt ttacgctccc
     3781 ttaacttgcc ctcattccca aacctcaatc gcgcgcgtat agtagcagcg tttaaaagct
     3841 ctaacttttg ttgcattacc gggatgtaaa gtgaatacaa cgctgtttcg atggccggtt
     3901 cgcgtctact atgaagatac cgatgccggt ggtgtggtgt accacgccag ttacgtcgct
     3961 ttttatgaaa gagcacgcac agagatgctg cgtcatcatc acttcagtca gcaggcgctg
     4021 atggctgaac gcgttgcctt tgtggtacgt aaaatgacgg tggaatatta cgcacctgcg
     4081 cggctcgacg atatgctcga aatacagact gaaataacat caatgcgtgg cacctctttg
     4141 gttttcacgc aacgtattgt caacgccgag aatactttgc tgaatgaagc agaggttctg
     4201 gttgtttgcg ttgacccact caaaatgaag cctcgtgcgc ttcccaagtc tattgtcgcg
     4261 gagtttaagc agtgactgac atgaatatcc ttgatttgtt cctgaaggct agccttctgg
     4321 ttaaacttat catgttgatt ttgattggtt tttcaatcgc atcctgggcc attattatcc
     4381 agcggacccg tattcttaac gcagcggcgc gcgaagccga agcgtttgaa gataaattct
     4441 ggtctggaat cgaactctct cgcctctatc aagagagtca ggggaaacgg gataatctga
     4501 ctggttcgga acaaatattt tacagcgggt tcaaagagtt tgtgcgcctg catcgtgcca
     4561 atagccatgc gccggaagcc gtagtggaag gggcgtcgcg tgctatgcgt atctccatga
     4621 accgtgaact tgaaaatctg gaaacgcaca ttccgttcct cggtacggtt ggctccatca
     4681 gcccgtatat tggtctgttt ggtacggtct gggggatcat gcacgccttt atcgccctcg
     4741 gggcggtaaa acaagcaaca ctgcaaatgg ttgcgcccgg tatcgcagaa gcgttgattg
     4801 cgactgcaat tggtctgttc gccgcaattc cggcggtaat ggcttataac cgcctcaacc
     4861 agcgcgtaaa caaactggaa ctgaattacg acaactttat ggaagagttt accgcgattc
     4921 tgcaccgcca ggcgtttacc gttagcgaga gcaacaaggg gtaagccatg gccagagcgc
     4981 gtggacgagg tcgtcgcgat ctcaagtccg aaatcaacat tgtaccgttg ctggacgtac
     5041 tgctggtgct gttgctgatc tttatggcga cagcgcccat catcacccag agcgtggagg
     5101 tcgatctgcc agacgctact gaatcacagg cggtgagcag taacgataat ccgccagtga
     5161 ttgttgaagt gtctggtatt ggtcagtaca ccgtggtggt tgagaaagat cgcctggagc
     5221 gtttaccacc agagcaggtg gtggcggaag tgtccagccg tttcaaggcc aacccgaaaa
     5281 cggtctttct gatcggtggc gcaaaagatg tgccttacga tgaaataatt aaagcactga
     5341 acttgttaca tagtgcgggt gtgaaatcgg ttggtttaat gacgcagcct atctaaacat
     5401 ctgcgtttcc cttgcttgaa agagagcggg taacaggcga acagtttttg gaaaccgaga
     5461 gtgtcaaagg caaccgaaca aaacgacaag ctcaagcggg cgataattat ttcagcagtg
     5521 ctgcatgtca tcttatttgc ggcgctgctc tggagttcgt tcgatgagaa tatagaagct
     5581 tcagccggag gcggcggtgg ttcgtccatc gacgctgtca tggttgattc aggtgcggta
     5641 gttgagcagt acaaacgcat gcaaagccag gaatcaagcg cgaagcgttc tgatgagcag
     5701 cgcaagataa aggaacagca ggctgctgaa gaactgcgtg agaaacaagc ggctgaacag
     5761 gaacgcctga agcaacttga gaaagagcgg ttagctgctc aggaacagaa aaagcaggct
     5821 gaagaagccg caaaacaggc cgagttaaag cagaagcaag cggaagaggc ggcagcgaaa
     5881 gcggcggcag atgctaaagc gaaggccgaa gcggatgcta aagctgcgga agaagcagcg
     5941 aagaaagcgg cggcagacgc gaagaaaaaa gcagaagcag aagccgccaa agccgcagcc
     6001 gaagcgcaga aaaaagccga ggcagccgct gcggcactga agaagaaagc ggaagcggca
     6061 gaagctgctg cagctgaagc aagaaagaaa gcggcagcag agaaagctgc agccgacaaa
     6121 aaagcagcag aaaaagcggc tgctgataag gcagcagctg ataagaaagc agcggcagaa
     6181 aaagccgccg cagacaaaaa agcggcagcg gcaaaagcag cggcagaaaa agccgccgca
     6241 gacaaaaaag cggcagcggc aaaagcagca gctgaaaaag ccgctgcagc aaaagctgcc
     6301 gcagaggcag atgatatttt cggtgagcta agctctggta agaatgcacc gaaaacgggg
     6361 ggaggggcga aagggaacaa tgcttcgcct gccgggagtg gtaatactaa aaacaatggc
     6421 gcatcagggg ccgatatcaa taactatgcc gggcagatta aatctgctat cgaaagtaag
     6481 ttctatgacg catcgtccta tgcaggcaaa acctgtacgc tgcgcataaa actggcaccc
     6541 gatggtatgt tactggatat caaacctgaa ggtggcgatc ccgcactttg tcaggctgcg
     6601 ttggcagcag ctaaacttgc gaagatcccg aaaccaccaa gccaggcagt atatgaagtg
     6661 ttcaaaaacg cgccattgga cttcaaaccg taatcgcgat gttgactgtt ccgacggtca
     6721 acatcaggca ccggttgcca cggggttctg gtagttttgt gtattttagt ttgttaacat
     6781 tctgctaaat tatcgtgggc catcggtcca gataagggag atatgatgaa gcaggcatta
     6841 cgagtagcat ttggttttct catactgtgg gcatcagttc tgcatgctga agtccgcatt
     6901 gtgatcgaca gcggtgtaga ttccggtcgt cctattggtg ttgttccttt ccagtgggcg
     6961 ggccctggtg cggcacctga agatattggc ggcatcgttg ctgccgactt gcgtaacagc
     7021 ggtaaattta atccgttaga tcgcgctcgt ctgccacagc agccgggtag tgcgcaggaa
     7081 gtacaaccag ctgcatggtc cgcgctgggt attgacgctg tagttgtcgg tcaggtcact
     7141 ccgaatccgg atggttctta caatgttgct tatcaacttg ttgacactgg cggcgcaccg
     7201 ggtactgtac ttgctcagaa ctcgtacaaa gtgaacaagc agtggctgcg ctatgctggt
     7261 cataccgcca gtgatgaagt gtttgaaaaa ctgaccggca ttaaaggtgc gttccgtacc
     7321 cgtattgcct acgttgttca gaccaacggc ggtcagttcc cgtatgaact gcgcgtatct
     7381 gactatgacg gttacaacca gtttgtcgtt caccgttcac cacagccgct gatgtccccg
     7441 gcgtggtcac cagacggttc taaactggct tatgtgacct tcgaaagcgg tcgttccgcg
     7501 ctagttatcc aaacgctggc aaacggcgct gtacgtcagg tggcttcatt cccgcgtcac
     7561 aacggtgcgc ctgcattctc gccagacggc agcaaactgg cattcgcctt gtcgaaaacc
     7621 ggtagcctga acctgtacgt aatggatttg gcttctggtc agatccgcca ggtgactgat
     7681 ggtcgcagta acaataccga accgacctgg ttcccggaca gccagaacct ggcatttact
     7741 tctgaccagg ccggtcgtcc acaggtttat aaagtgaata tcaacggcgg tgcgccacaa
     7801 cgtattacct gggaaggttc gcagaaccag gatgcggatg tcagcagcga cggtaaattt
     7861 atggtaatgg tcagctccaa cggtgggcag cagcacattg ccaaacaaga tctggcaacg
     7921 ggaggcgtac aagttctgtc gtccacgttc ctggatgaaa cgccaagtct ggcacctaac
     7981 ggcactatgg taatctacag ctcttctcag gggatgggat ccgtgctgaa tttggtttct
     8041 acagatgggc gtttcaaagc gcgtcttccg gcaactgatg gacaggtcaa attccctgcc
     8101 tggtcgccgt atctgtgata ataattaatt gaatagtaaa ggaatcattg aaatgcaact
     8161 gaacaaagtg ctgaaagggc tgatgattgc tctgcctgtt atggcaattg cggcatgttc
     8221 ttccaacaag aacgccagca atgacggcag cgaaggcatg ctgggtgccg gcactggtat
     8281 ggatgcgaac ggcggcaacg gcaacatgtc ttccgaagag caggctcgtc tgcaaatgca
     8341 acagctgcag cagaacaaca tcgtttactt cgatctggac aagtacgata tccgttctga
     8401 cttcgctcaa atgctggatg cacatgcaaa cttcctgcgt agcaacccgt cttacaaagt
     8461 caccgtagaa ggtcacgcgg acgaacgtgg tactccggaa tacaacatct ccctgggtga
     8521 acgtcgtgcg aacgccgtta agatgtacct gcagggtaaa ggcgtttctg cagaccagat
     8581 ctccatcgtt tcttacggta aagaaaaacc tgcagtactg ggtcatgacg aagcggcata
     8641 ctccaaaaac cgtcgtgcgg tactggttta ctaagagaat tgcatgagca gtaacttcag
     8701 acatcaacta ttgagtctgt cgttactggt tggtatagcg gccccctggg ccgcttttgc
     8761 tcaggcacca atcagtagtg tcggctcagg ctcggtcgaa gaccgcgtca ctcaacttga
     8821 gcgtatttct aatgctcaca gccagctttt aacccaactc cagcaacaac tttctgataa
     8881 tcaatccgat attgattccc tgcgtggtca gattcaggaa aatcagtatc aactgaatca
     8941 ggtcgtggag cggcaaaagc agatcctgtt gcagatcgac agcctcagca gcggtggtgc
     9001 agcggcgcaa tcaaccagca gcgatcaaag cggtgcggcg gcatcaacga cgccgacagc
     9061 tgatgctggt actgcgaatg ctggcgcgcc ggtgaaaagc ggcgatgcaa acacggatta
     9121 caatgcagct attgcgctgg tgcaggataa atcccgccag gatgacgcaa tggtggcatt
     9181 tcagaatttc atcaaaaatt accctgattc aacttacctg ccaaacgcca attattggct
     9241 gggtcagtta aactacaaca agggtaaaaa agatgatgcg gcgtactatt ttgcttcggt
     9301 agtgaaaaac tatccgaagt caccaaaggc tgctgatgcg atgtttaaag tcggcgtcat
     9361 catgcaggac aaaggtgaca ccgcaaaagc gaaagccgta taccagcagg ttatcagtaa
     9421 ataccctggt actgatggcg ctaaacaggc acaaaaacgt ctgaacgcga tgtaatgcat
     9481 aacacacgac cagaagtcgc attatttctg gtcgtgtcgt gcgaatcata agcagttgag
     9541 tgatctgcat cgaaattttt gttgcgctca agtctgaaat cagtaatata tgccgccgtt
     9601 gccacgggat atcaaacaaa ccgaaagcaa cgaaaaagtg ggtcgttagc tcagttggta
     9661 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccagtttt
     9721 agcatcgaag acagatgtta agcgtgtagg ataacgttgc gtcagcaacg gcccgtaggg
     9781 cgagcgaagc gagtcatcct gcacgaccca ccactaatta cggtgggttc ggtggaagta
     9841 gtttgtagta tccagcgcag tatcgggtga ttagctcagc tgggagagca cctcccttac
     9901 aaggaggggg tcggcggttc gatcccgtca tcacccacca ctcgggtcgt tagctcagtt
     9961 ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa
    10021 ttttaacatc aaactcagat gttaagcgtg aaggataacg ttgcgccagc aacggcccgt
    10081 agggcaagcg aagcgagtca tcctgcacga cccaccaatc ttaaagattg gccccgagta
    10141 gaaatctttc aggtaacacc cgtatgggtc gttagctcag ttggtagagc agttgacttt
    10201 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc atcctgaatg attaaagcag
    10261 cataatcccg caaggggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
    10321 tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt
    10381 tgctatctga gatattcaaa gattcgaacc tgcagcaggt tcgagttgag cgcagcgaaa
    10441 caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat cctgcacgac
    10501 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct attcaggcat cctcaatttc
    10561 actttgtaaa cctgatgaca tcgtcagagc ttactgtgca agcaactcta cgtcggtgga
    10621 attaggcgta attcgagtga cttttcccca ccattcgact atcttgttta gcatataaaa
    10681 caaattacgc cgatagtagc gaatattacg ctaatgtcgg ttttaacgtt aagcctgtaa
    10741 aacgagatgg taagatgagc gtgatgtttg atccagacac ggcgatttat cctttccccc
    10801 cgaagccgac gccgttaagc attgatgaaa aagcgtatta ccgcgagaag ataaaacgtc
    10861 tgctaaaaga acgtaatgcg gtgatggttg cccactacta taccgacccc gaaattcaac
    10921 aactggcaga agaaaccggt ggctgtattt ctgattctct ggaaatggcg cgcttcggtg
    10981 caaagcatcc cgcttctacg ttgttagtcg ctggggtgag atttatggga gaaaccgcca
    11041 aaattctcag tccggaaaaa acaattctga tgctgacact tcaggctgaa tgttcactgg
    11101 atctcggctg ccctgttgaa gaatttaacg cattttgcga tgcccatccc gatcatactg
    11161 tcgtcgtcta cgccaacact tctgctgcgg taaaagcgcg cgcagattgg gtggtaactt
    11221 caagcattgc cgtcgaactt attgatcatc tggatagttt tggtgaaaaa atcatctggg
    11281 cacccgacaa acatctgggg cgttacgtgc aaaaacagac gggcgcagac attttatgct
    11341 ggcagggtgc ctgtattgtg catgatgaat ttaagactca ggcgttaacc cgcttgcaag
    11401 aagaataccc ggatgctgcc atactggtgc atccagaatc accacaagct attgtcgata
    11461 tggcggatgc ggtcggttcc accagtcaac tgatcgctgc tgcgaaaaca ttgccacatc
    11521 agcggcttat cgtggcaacc gatcggggca ttttctacaa aatgcagcag gcggtgccag
    11581 ataaagagtt actggaagca ccaaccgcag gtgagggtgc aacctgccgc a
//
LOCUS       DAZPLM010000129        11469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPLM010000129 DAZPLM010000000
VERSION     DAZPLM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.129"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..611
                     /locus_tag="ACHVC7_002898"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHVC7_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /gene="prfH"
                     /locus_tag="ACHVC7_002899"
     CDS             608..1222
                     /gene="prfH"
                     /locus_tag="ACHVC7_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR2116890.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHVC7_002900"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHVC7_002900"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR2116891.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="ACHVC7_002901"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="ACHVC7_002901"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR2116892.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="ACHVC7_002902"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="ACHVC7_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR2116893.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="ACHVC7_002903"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="ACHVC7_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR2116894.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHVC7_002904"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHVC7_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR2116895.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="ACHVC7_002905"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="ACHVC7_002905"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR2116896.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="ACHVC7_002906"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="ACHVC7_002906"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR2116897.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="ACHVC7_002907"
     tRNA            9115..9190
                     /locus_tag="ACHVC7_002907"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="ACHVC7_002908"
     CDS             9796..9954
                     /locus_tag="ACHVC7_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116898.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2971 a         2670 c         2893 g         2935 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga ttaaaatata aacagataat tatgtatctt attgatttat
    10201 aaggttattt ttaagtaaag gcaaataaaa aataaatagc cataaatagc tataaataca
    10261 tcttaatacc tatatccctt cccttatggc tagattgcga gaggtgctta acgcgatttt
    10321 cagcgccccc cgatctggaa aatccagaat ccccaaatat cagcaaggaa ttttcttgcg
    10381 taatcaacat cgtttttacc tattaaatca ttacacagaa agcctttctg taatgtctct
    10441 cactaaactt tcactaaaca cgagaatgat cggttttatg tcggatcaga tgctagaaac
    10501 agcaccacgt ctcacaaggg ctgtaagcga tgaaaccagt gtttatgcgg gtgcaggtca
    10561 aaatataggc caaaatccac ttaacataat aaatgttata tgtgctaagg aacacatata
    10621 acgccttgaa ttgatgtatc aggggaaaag tgatggattt tttacgtagg agcacgaaag
    10681 ggcttctatt gacttttgta tgctatgaaa tataattaaa gttttttgtt tttattgtgt
    10741 gatgtggtgg gttacacagg ggaatagatt aaatggtgtt ctgtttatac aggtttattt
    10801 gttagatcaa caatttggca tttctaaaca gatgtttgaa cgttgccaaa tgtcttgaca
    10861 acacaagaaa agggcttgct atgccaggca ttacacgaaa taaggctata aaaacaaaat
    10921 aaattttaaa atttgcacaa acgagaaact cgttcagaaa caagcagaac aatttttagc
    10981 caacaatttc tgatggtgct ctgtaagccg cattacacaa ggctttgcga aagtgatgta
    11041 aaagtgaaga aagtaagaat tgaactttta agctactttt cattggattt tttagtaggg
    11101 aattacacag ggaatagaga gcattattct atgatttaca cagggaatat agggggatta
    11161 tggcacacac atagaaagac attacacaga gtgctatgtg acaaatggat gtaacgagag
    11221 gttacattca tttatctgaa atgctctctg taagaaagtt ggtagtaacg atatgttaca
    11281 tagactaatt attgaatgta acgatattac acatcggtag taacgatacg ttacatcgga
    11341 agatgattgg atgtaacggt agctcacagt gaactagggg taggtcatag tgaactaccc
    11401 ccacagaatt ctgttacccc tatcaagtct gataccccta tcaaaactga tacccgataa
    11461 gtataaata
//
LOCUS       DAZPLM010000130        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPLM010000130 DAZPLM010000000
VERSION     DAZPLM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.130"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHVC7_002909"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHVC7_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR2116899.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHVC7_002910"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHVC7_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR2116900.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHVC7_002911"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHVC7_002911"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR2116901.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHVC7_002912"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHVC7_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR2116902.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHVC7_002913"
     CDS             complement(3928..5214)
                     /locus_tag="ACHVC7_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR2116903.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHVC7_002914"
     CDS             complement(5235..6026)
                     /locus_tag="ACHVC7_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR2116904.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHVC7_002915"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHVC7_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR2116905.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHVC7_002916"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHVC7_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR2116906.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHVC7_002917"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHVC7_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR2116907.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHVC7_002918"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHVC7_002918"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR2116908.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHVC7_002919"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHVC7_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR2116909.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHVC7_002920"
     CDS             complement(9882..10364)
                     /locus_tag="ACHVC7_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR2116910.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHVC7_002921"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHVC7_002921"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR2116911.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPLM010000131        11408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPLM010000131 DAZPLM010000000
VERSION     DAZPLM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.131"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHVC7_002922"
     CDS             460..1386
                     /locus_tag="ACHVC7_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR2116912.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHVC7_002923"
     CDS             complement(1336..2529)
                     /locus_tag="ACHVC7_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2116913.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHVC7_002924"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHVC7_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR2116914.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHVC7_002925"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHVC7_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR2116915.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHVC7_002926"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHVC7_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR2116916.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHVC7_002927"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHVC7_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR2116917.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHVC7_002928"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHVC7_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR2116918.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHVC7_002929"
     CDS             complement(11165..11308)
                     /locus_tag="ACHVC7_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2116919.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2880 a         2877 c         2934 g         2717 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataa
//
LOCUS       DAZPLM010000132        11297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPLM010000132 DAZPLM010000000
VERSION     DAZPLM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.132"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            90..165
                     /locus_tag="ACHVC7_002930"
     tRNA            90..165
                     /locus_tag="ACHVC7_002930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Val,seq:tac)
     gene            170..245
                     /locus_tag="ACHVC7_002931"
     tRNA            170..245
                     /locus_tag="ACHVC7_002931"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:203..205,aa:Lys,seq:ttt)
     gene            276..401
                     /locus_tag="ACHVC7_002932"
     ncRNA           276..401
                     /ncRNA_class="other"
                     /locus_tag="ACHVC7_002932"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            521..862
                     /locus_tag="ACHVC7_002933"
     CDS             521..862
                     /locus_tag="ACHVC7_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR2116920.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(853..1779)
                     /locus_tag="ACHVC7_002934"
     CDS             complement(853..1779)
                     /locus_tag="ACHVC7_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2116921.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1869..2867
                     /locus_tag="ACHVC7_002935"
     CDS             1869..2867
                     /locus_tag="ACHVC7_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR2116922.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2864..3082)
                     /locus_tag="ACHVC7_002936"
     CDS             complement(2864..3082)
                     /locus_tag="ACHVC7_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR2116923.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(3084..5099)
                     /gene="ligA"
                     /locus_tag="ACHVC7_002937"
     CDS             complement(3084..5099)
                     /gene="ligA"
                     /locus_tag="ACHVC7_002937"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR2116924.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(5170..6156)
                     /gene="zipA"
                     /locus_tag="ACHVC7_002938"
     CDS             complement(5170..6156)
                     /gene="zipA"
                     /locus_tag="ACHVC7_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR2116925.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6386..7147
                     /gene="cysZ"
                     /locus_tag="ACHVC7_002939"
     CDS             6386..7147
                     /gene="cysZ"
                     /locus_tag="ACHVC7_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR2116926.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7332..8303
                     /gene="cysK"
                     /locus_tag="ACHVC7_002940"
     CDS             7332..8303
                     /gene="cysK"
                     /locus_tag="ACHVC7_002940"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR2116927.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8687..8944
                     /gene="ptsH"
                     /locus_tag="ACHVC7_002941"
     CDS             8687..8944
                     /gene="ptsH"
                     /locus_tag="ACHVC7_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR2116928.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8989..10716
                     /gene="ptsI"
                     /locus_tag="ACHVC7_002942"
     CDS             8989..10716
                     /gene="ptsI"
                     /locus_tag="ACHVC7_002942"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR2116929.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10757..11266
                     /gene="crr"
                     /locus_tag="ACHVC7_002943"
     CDS             10757..11266
                     /gene="crr"
                     /locus_tag="ACHVC7_002943"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR2116930.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2750 a         2916 c         2809 g         2822 t
ORIGIN      
        1 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccaatttctc gccagctaaa
       61 tttcttgtaa aaatgtgaag taccgaagtg ggtgattagc tcagctggga gagcacctcc
      121 cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc
      181 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
      241 caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac tcaaagattc
      301 gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg cggcgacggc
      361 ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgtaaaaa agcgccctaa
      421 aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag ttccttgttc
      481 ttctgccgat attacctcgt acagtgacga ggactgaaca atggccacta tctcttcaac
      541 ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta gcgatgttgc
      601 ttcaaaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa ttaaagagat
      661 tgtagacact agcggcagtg ctgaagacaa acaaaaacag gccgaattga ttcaacagca
      721 aatcacccta ctggaaacac aactggcgca gttgcagaga cagcaggcag aaaaggcgca
      781 ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg aaaatacgac
      841 tcatatcgat atctatatct gatacaacgg agccccttcc caaccagcca ttagcgcatt
      901 gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg aacggttctt
      961 cctgcgcacc agcatcaact ggcgttcaac aaccggcgta atgcgcttta ccaccagtgg
     1021 gctaccttca ggcaatggta gcgccagtgc gggaagaata ctgatgccga tgcctgcggc
     1081 taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg cctgaatacc
     1141 attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat cctgcaacac
     1201 caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca aagcgctatc
     1261 gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca gatcgcccac
     1321 tgggcctgga tcgatgacga tgcaaaaatc gacatctccc tggcgaatac tttccatcac
     1381 ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct ggatagcggc gatggctttc
     1441 agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga cccgaacttt
     1501 tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa gttcatccag
     1561 cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca cttcacgcgt
     1621 ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac tgtgacttac
     1681 tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct cctgcgctac
     1741 tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc atgaatggat
     1801 gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat cgatcatctg
     1861 gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg atcacggtgg
     1921 tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt gaaaatctga
     1981 ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga
     2041 ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc accttcgtgc
     2101 tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc
     2161 tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct gcaatcgcct
     2221 tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca gcatccagcc
     2281 tgctggggat tttcctttca ccattgctag ttggtctggt gatgaatgtt cacggtgcag
     2341 ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg tttgtgttgg
     2401 ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa tggattgcga
     2461 aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa gccgtcgtta
     2521 atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg gtcagctgcg
     2581 ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg ggcttcaata
     2641 aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc
     2701 cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg cccctgatga
     2761 ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac aaacgccaga
     2821 ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc ggacgcttca
     2881 ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt tgcattagct
     2941 caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga tactcctccg
     3001 gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg cattatcgta ttggctattt
     3061 caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc cgcttcgtcg
     3121 atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc ttcacccgct
     3181 atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt
     3241 cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac cacggtttta
     3301 ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg ccagtgaaca
     3361 ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa gaagttgtga
     3421 acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc aatcgaagcg
     3481 gcttccagca cttccagcgt gccgaaatat gccgccagac ctgctgcggt ggcctcgccg
     3541 acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt cgctttttcc
     3601 agcgcgttaa ccacgttttg tgccgatttt ggccccatac gctccagtcc ggtcagtttg
     3661 cctgcggtga gtttgaacag atctgccgga gtgtggacat attctttttc aaccagctga
     3721 tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc
     3781 agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc aaccgcttca
     3841 ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa tacaacctca
     3901 cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat cacgtcgcca
     3961 gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat ttcatccgca
     4021 ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc cagacgcgca
     4081 acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc
     4141 tgctcctgcg ccgggaattt aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca
     4201 agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat atcaaagccc
     4261 agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc cgccgattca
     4321 caaagcgtta cccgatcgct gaccggcaac ccccactttt taaattgcag taaacggcca
     4381 agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca gaaaaaagtg
     4441 agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc tgccgcatta
     4501 cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac
     4561 cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat attctctccg
     4621 tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc ttccccggtg
     4681 gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag aatactgacg
     4741 gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt tttcagacgg
     4801 tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt atccagtgac
     4861 agcattggta cttcatggcg tatctggctg aaagccgcca gcggcgcagc acctacacgt
     4921 tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc gcgcagttcg
     4981 cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac atgataaaga
     5041 tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat tgattccata
     5101 tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag ttaatttgcc
     5161 ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt actcgcgcaa
     5221 tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca cttcatcggc
     5281 aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac cgtaagacgg
     5341 tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag gatcaaaggt
     5401 tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat ccgggctaag
     5461 atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag
     5521 aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga taatcaccgc
     5581 ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct caggctgtgg
     5641 tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac
     5701 aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct gttcaggctg
     5761 cggctgatag gcaggctgct gcatcggctg cgctggacgc ggagcatgtt gcggcggtac
     5821 ctgcgcttca ggcggctgct ggatcggttg acgcggctgc gcagacgcat aaggcggttg
     5881 gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag ccggggcatg
     5941 attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat cctcgtcata
     6001 agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga acatagaaga
     6061 tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg cgatcgcgcc
     6121 aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg ttctaacacc
     6181 ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc aagtgcaagt
     6241 gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct gttttttcga
     6301 acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt ttcctgggat
     6361 tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc cacgcagcgg
     6421 tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc ggcgtttcgt
     6481 tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt ggctctttac
     6541 acagctcgat gtctggatcc cgactctcat gagttacgtt ccggactggc tacaatggct
     6601 gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg gctatttctt
     6661 ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg aacaactgga
     6721 agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca tgaaagatgt
     6781 gccgcgaatc atgaaacgcg aatggcaaaa atttgcctgg tatctgccgc gcgcaattgt
     6841 attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg tactgtggtt
     6901 cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg ataaccacaa
     6961 agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca atatgcagtt
     7021 tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca tcatgcccgt
     7081 tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac acgcgatgtg
     7141 gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg tggcttatgc
     7201 cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata tgctaaatcc
     7261 ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt tcatacagtt
     7321 aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt cacacgccgc
     7381 tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa tctcgtaacc
     7441 ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc gaaaagcgcg
     7501 gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc gggattgcac
     7561 tggcctatgt tgctgccgct cgcggttaca aactcaccct gaccatgcca gaaaccatga
     7621 gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctagtgctg acggaaggtg
     7681 ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc aatccagaga
     7741 aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac gaaaaaacca
     7801 ccggtccgga gatctgggaa gataccgacg gtcaggttga tgtatttatt gctggcgttg
     7861 ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa ggcaagaccg
     7921 atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag gcgctggcag
     7981 gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt tttatcccgg
     8041 ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa gaagccattt
     8101 ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct tctggagcag
     8161 ctgttgccgc ggcgttgaaa ctacaagaag atgaaagctt taccaataag aatattgtgg
     8221 ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc gatctcttca
     8281 ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa agcacctttt
     8341 taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga ttcagcctgt
     8401 cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg ttacaggaaa
     8461 agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttaacgg cataatgttt
     8521 aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat tgcgctcttc
     8581 gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga accgccaggc
     8641 tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt tccagcaaga
     8701 agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt ttgtaaaaga
     8761 agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg ccagcgcgaa
     8821 aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga ctatctccgc
     8881 agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg cggaactcga
     8941 gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat gatttcaggc
     9001 attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga agacgaaatt
     9061 gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga acgttttctg
     9121 agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc tggtgaaacg
     9181 ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga agatgaggag
     9241 ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga cgcagctgct
     9301 catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga atacctgaaa
     9361 gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat cctgggcctg
     9421 aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc tgacctgacg
     9481 ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac cgacgcgggt
     9541 ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc tatcgtgggt
     9601 accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga tgccgtaaat
     9661 aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc tgttcaggag
     9721 caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc tattacgctg
     9781 gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt tgaaggtgca
     9841 gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt catggaccgc
     9901 gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc tgaagcgtgt
     9961 ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga gctgccatac
    10021 atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg tatcgcgatg
    10081 gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc ggctttcggt
    10141 aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc actgcgcaaa
    10201 gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga cgagtcaatt
    10261 gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca tttagccaaa
    10321 gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct ggcagttgac
    10381 cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt gctgaacttg
    10441 atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat gtgtggtgag
    10501 cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga cgaattctct
    10561 atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa cttcgaagat
    10621 gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt aatgacgctg
    10681 gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg cccaatttac
    10741 tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt ccgacgacaa
    10801 gaaggatacc ggaactattg agatcattgc tccgctctct ggcgagatcg tcaatatcga
    10861 agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg ctatcaaacc
    10921 aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct ttgaaaccaa
    10981 tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact tcggtatcga
    11041 caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc agcgcgtgaa
    11101 agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag ccaagtctac
    11161 cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca aactgtccgg
    11221 tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct tgccgcagtg
    11281 aaaaatggcg cccatcg
//
LOCUS       DAZPLM010000133        11296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPLM010000133 DAZPLM010000000
VERSION     DAZPLM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.133"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHVC7_002944"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHVC7_002944"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR2116931.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHVC7_002945"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHVC7_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR2116932.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHVC7_002946"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHVC7_002946"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR2116933.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHVC7_002947"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHVC7_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR2116934.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHVC7_002948"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHVC7_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR2116935.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHVC7_002949"
     CDS             complement(6652..8160)
                     /locus_tag="ACHVC7_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR2116936.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHVC7_002950"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHVC7_002950"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR2116937.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHVC7_002951"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHVC7_002951"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR2116938.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2651 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg cccttc
//
LOCUS       DAZPLM010000134        11287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPLM010000134 DAZPLM010000000
VERSION     DAZPLM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.134"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHVC7_002952"
     CDS             79..213
                     /locus_tag="ACHVC7_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR2116939.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHVC7_002953"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHVC7_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR2116940.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHVC7_002954"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHVC7_002954"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR2116941.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHVC7_002955"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHVC7_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR2116942.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHVC7_002956"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHVC7_002956"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR2116943.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAVLSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHVC7_002957"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHVC7_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR2116944.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHVC7_002958"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHVC7_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2116945.1"
                     /translation="MKLNIRAQTAQNQHNNSPIALVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHVC7_002959"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHVC7_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR2116946.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHVC7_002960"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHVC7_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR2116947.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHVC7_002961"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHVC7_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR2116948.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHVC7_002962"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHVC7_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR2116949.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHVC7_002963"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHVC7_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR2116950.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHVC7_002964"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHVC7_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2761 a         2777 c         2808 g         2941 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 caccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ctcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtga
//
LOCUS       DAZPLM010000135        11244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPLM010000135 DAZPLM010000000
VERSION     DAZPLM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.135"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHVC7_002965"
     CDS             <1..311
                     /locus_tag="ACHVC7_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR2116951.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            510..791
                     /gene="ppiC"
                     /locus_tag="ACHVC7_002966"
     CDS             510..791
                     /gene="ppiC"
                     /locus_tag="ACHVC7_002966"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR2116952.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHVC7_002967"
     CDS             complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHVC7_002967"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR2116953.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHVC7_002968"
     CDS             2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHVC7_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR2116954.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHVC7_002969"
     CDS             complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHVC7_002969"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR2116955.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHVC7_002970"
     CDS             complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHVC7_002970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR2116956.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHVC7_002971"
     CDS             complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHVC7_002971"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR2116957.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHVC7_002972"
     CDS             complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHVC7_002972"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR2116958.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHVC7_002973"
     CDS             complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHVC7_002973"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR2116959.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHVC7_002974"
     CDS             complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHVC7_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR2116960.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHVC7_002975"
     CDS             complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHVC7_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR2116961.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10356..>11244
                     /locus_tag="ACHVC7_002976"
     CDS             10356..>11244
                     /locus_tag="ACHVC7_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR2116962.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2649 t
ORIGIN      
        1 ttgtcgtgct caacggaaaa attaatggca acgcatgtat cgtcgttcca ttatcaacca
       61 ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga aatagccagt aatgttatca
      121 atgacctgca attctttgat cagcagattc gttgggccaa agccgaccta gtacaacaag
      181 tcagtcgaaa ccgattaaac agagcaagga cttacagagg gtatcttaat caatgtttgc
      241 cacatgagtt ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc
      301 tcataaattg acaataaaat aaccatcccc gataatgact ttgtagccca tagaggccct
      361 aagttttctc cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg
      421 taccagcccc tccctgacta acaattacgt tagcatctgc ggatcatgct aaaatcgccg
      481 cctgacaatt attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
      541 ttgtaaaaga agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt
      601 tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag
      661 gtgaattccg ccagggtcag atggttccgg cgtttgataa agttgtgttc tcttgtccgg
      721 tactggagcc aaccggcccg ctgcacaccc agttcggtta ccacatcatt aaggtgctgt
      781 accgtaacta atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc
      841 tgtgggagag ggtcggggtg agggcatcag cgcgcactta acccgcaact gcaatacgtt
      901 tcatatccgt catatagccg cgcagtttct tacctacctg ctcaatcgca tggcagcgaa
      961 tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc accttccgga atagctttac
     1021 ccagatcgcc cggttgcagc tctgccataa acggtttcag cagcggcaca caagcgtaag
     1081 agaacagata gttaccgtac tcggcggtat cagagataac cacgttcatt tcgtacagac
     1141 gcttacgggc gatggtgttg gcaatcagcg gcagttcgtg cagtgattca tagtaagcag
     1201 attcttcgat gatgccggaa tcaaccatgg tttcaaacgc cagctcaacg cccgctttca
     1261 ccatcgcgat catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat
     1321 actgcggcgc ggtttcgaag gcggttttgc cggtctcttc acgccaggtc agcagtttct
     1381 tatcatcgtt ggcccagtcc gccatcatac cggaggagaa ttcgccggag atgatgtcgt
     1441 ccatatgttt ctggaacagg ggtgccatga tctctttcag ctgttcagac agcgcgtaag
     1501 cacgcagttt cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca
     1561 gcgcttcggt gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg
     1621 tgccctcttc caccagcttg tcgaagcaca gcagagagcc agcctgcaac ataccgcaca
     1681 ggatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca
     1741 cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc atgccttcgc
     1801 ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt
     1861 actcttcacg tacttcggtg ccagggcatt tcggcgcaac catgactacg gtaatgtctt
     1921 tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc
     1981 cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tctggcgtca
     2041 ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt
     2101 tttcggtcgc tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt
     2161 aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac
     2221 agccgacgat aaccactttt ttaccctgaa ggtagctcgc gccgtcggcg aattcgtcgc
     2281 gccccataaa gcgacatttg cccagctgtg ccagctgctg gcgcaggttc agtgtattga
     2341 agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg
     2401 cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt
     2461 cacattgcaa tttctgcaac gtcaacatcg agggctgcgc cattggattt acgcgatctg
     2521 aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac
     2581 gttagcccat ccacgctctc acggcagatt cagcgcctgg aagaagatct tggtcagccg
     2641 ctatttgtgc gcgataaccg cacggtaacg ctgacggaag cgggcgaaga gctgcgcgtt
     2701 ttcgcccagc aaacgctgtt gcagtatcag caattgcgcc acaccatcga tcagcaaggg
     2761 ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta cagccatctg
     2821 ccgccgattc tggatcgctt ccgcgcggaa cacccgtcgg tggagatcaa actcaccact
     2881 ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc
     2941 gggaaaccgg agacgctacc cggcgctgtg gcgttttcga tgctggagaa tctggcagta
     3001 gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc aggtatctgt cgaaaagccc
     3061 gactggtcaa cggtgccgtt tattatggcc gatcaggggc cggtacgccg ccgcattgaa
     3121 ctgtggtttc gacgcaataa aatcagcaac ccgatgattt acgccacggt tggcgggcat
     3181 gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtggtg
     3241 ctggaaaaca gcccggagcc tgtacgcaac cgggtaatga ttttagatcg cagcgatgag
     3301 aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt
     3361 gaggcgttct ggaggatttt gccgaaccac aaatgacgtt gtcgcgcggg taggcctgat
     3421 aagcgaagcg ctatcaggca tttttcccta acccgccaaa aagaacctga acgccggatt
     3481 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3541 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3601 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3661 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3721 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3781 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3841 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3901 gaccgatcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3961 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     4021 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccaggcgtt cgccgcgaat
     4081 gttgtgctga gcgatatatt ttttcattcc cgccagcgcc aacgcgccag agggttccgc
     4141 cactgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4201 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcgc cgatgcgttt
     4261 taccgcgaca ccttcggcga acagtcccac gcgcggcaga tcaaccggat gacccgcatc
     4321 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4381 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4441 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4501 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4561 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4621 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4681 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4741 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4801 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4861 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgccg cctcgtaaac
     4921 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg
     4981 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     5041 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5101 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5161 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5221 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5281 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5341 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5401 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5461 accgcgtcgt cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca
     5521 tcgacgcccg ctgttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5581 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5641 tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtagtacg aatgcccgca
     5701 gggcctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt
     5761 tccagagttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
     5821 gcgcgatcca gttcaccgag aataccgata acaccaccag cacggtgaac atcttccata
     5881 tggtattttt gggtgctcgg cgcaactttg cataactgcg ggactttacg cgaaagtttg
     5941 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     6001 accgtgttgg tagagccgcc catcgcgata tccagcgtca tggcattttc aaacgccgct
     6061 ttgctagcaa tattgcgcgg cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc
     6121 aattcaacaa tgcgtttacc ggcattgagg aacaactgct tacggtccgc gtgggttgcc
     6181 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6241 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6301 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6361 agcttgatga tctgctcgga cagtttggtt ttcccggctt ccatcgggcc gccggaaaca
     6421 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6481 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6541 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catagcaatc
     6601 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6661 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6721 gagttcacaa ccgcgataat cggcttgcca aaatcggcat cggtcattcc ggtggcgcgc
     6781 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6841 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtaagt gccgtcccat
     6901 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6961 gaagaggccg aagaaggctt gctgaatgca tttggtaacc gggccgcaac ggccttcacc
     7021 cacctgaata ccgtctacac tgcgcactgg cgtgatttct gccgccgtac cggacataaa
     7081 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7141 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7201 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7261 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7321 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7381 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7441 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7501 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7561 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7621 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7681 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7741 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7801 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7861 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7921 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7981 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     8041 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc tggattgaag cgagccgata
     8101 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8161 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8221 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8281 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8341 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8401 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8461 cacattcatc atgaaagagc cgtcaccgga gatacagaca acggtatcgt tcggtcgcgc
     8521 gacttgtgcg ccaaccgccg ccggtaaacc aaaacccatg gtacctaagc cgctggaggt
     8581 gatgaaattt tccgggcgag tgtgggcgat gtgctgcgca gcccacatct ggtgctgccc
     8641 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8701 cggcgcgtag atagcgtcac cgggatggtc gtaacgccag gaatgttcat cacgcagctg
     8761 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc
     8821 atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8881 gatatccata tggataacac tggcgtgtgg tgcgaaagta ttcagtttgc cggtcacccg
     8941 gtcatcaaaa cgtgcgccca cggcgatcag caggtcacac tcctgcaccg cgaagtttgc
     9001 cgctttgatg ccgtgcatcc ccagcatgcc cagatagtac ggataatctg cttctactgc
     9061 gcccagccct ttcagcgtac aggtggcagg catttttgtg gcagcgagaa attcacgcaa
     9121 agccggaact gcctgcgcca tacccacgcc accgccaacg tacagcatcg gtttttgcgc
     9181 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9241 aacggtggtg aaccacggtt ccaggtcacc gctggctaac tggatatctt ttgggatatc
     9301 gaccagaacc ggaccaggac gacctgagca ggcaacgtcg aatgcttcag ccatgatgcg
     9361 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9421 taggacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggaca cttgaccggt
     9481 gatggcaaca acagggatgg aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9541 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9601 gatagccgcc attgccgcac cctgctcatg tcggcatagc aagtgctcca cgccgccgtc
     9661 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9721 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
     9781 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9841 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9901 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9961 cgctaatcac gactaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    10021 cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac aagatatttt
    10081 tgggcctatt tttatgatag ctttaataat ttcaatagaa gttgttgttt tttaaaaaga
    10141 aaatagtgag tgaaaaaaat tcaatttcag tataaatgtt gttcccttcc tcgtagttca
    10201 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    10261 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    10321 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    10381 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    10441 tacccggctt aacgatggtg ggcttaccag aaacaactgt aaaagaagct cgcgatcgcg
    10501 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    10561 ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    10621 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
    10681 aactggctct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag
    10741 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    10801 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    10861 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    10921 atctcagtga tgttgtcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    10981 gcaggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    11041 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    11101 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    11161 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    11221 aaatttcgtt ggcgcataac ggcg
//
LOCUS       DAZPLM010000136        11216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPLM010000136 DAZPLM010000000
VERSION     DAZPLM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.136"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHVC7_002977"
     CDS             53..352
                     /locus_tag="ACHVC7_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR2116963.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHVC7_002978"
     CDS             complement(472..651)
                     /locus_tag="ACHVC7_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR2116964.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHVC7_002979"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHVC7_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR2116965.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHVC7_002980"
     CDS             2090..2668
                     /locus_tag="ACHVC7_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR2116966.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHVC7_002981"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHVC7_002981"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR2116967.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHVC7_002982"
     CDS             4347..5945
                     /locus_tag="ACHVC7_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR2116968.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHVC7_002983"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHVC7_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR2116969.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHVC7_002984"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHVC7_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR2116970.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHVC7_002985"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHVC7_002985"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR2116971.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHVC7_002986"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHVC7_002986"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR2116972.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHVC7_002987"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHVC7_002987"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR2116973.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHVC7_002988"
     CDS             10721..11179
                     /locus_tag="ACHVC7_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR2116974.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2756 a         2823 c         2829 g         2808 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca actcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcaatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tatttt
//
LOCUS       DAZPLM010000137        11085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPLM010000137 DAZPLM010000000
VERSION     DAZPLM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.137"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..393
                     /locus_tag="ACHVC7_002989"
     CDS             <1..393
                     /locus_tag="ACHVC7_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR2116975.1"
                     /translation="KPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVA
                     ADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQK
                     AIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            604..927
                     /locus_tag="ACHVC7_002990"
     CDS             604..927
                     /locus_tag="ACHVC7_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR2116976.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            924..1754
                     /gene="amiD"
                     /locus_tag="ACHVC7_002991"
     CDS             924..1754
                     /gene="amiD"
                     /locus_tag="ACHVC7_002991"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR2116977.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1751..2764)
                     /locus_tag="ACHVC7_002992"
     CDS             complement(1751..2764)
                     /locus_tag="ACHVC7_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR2116978.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2863..4293)
                     /locus_tag="ACHVC7_002993"
     CDS             complement(2863..4293)
                     /locus_tag="ACHVC7_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR2116979.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4304..5305)
                     /gene="ltaE"
                     /locus_tag="ACHVC7_002994"
     CDS             complement(4304..5305)
                     /gene="ltaE"
                     /locus_tag="ACHVC7_002994"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR2116980.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5342..7060)
                     /gene="poxB"
                     /locus_tag="ACHVC7_002995"
     CDS             complement(5342..7060)
                     /gene="poxB"
                     /locus_tag="ACHVC7_002995"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR2116981.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7193..8161)
                     /gene="hcr"
                     /locus_tag="ACHVC7_002996"
     CDS             complement(7193..8161)
                     /gene="hcr"
                     /locus_tag="ACHVC7_002996"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR2116982.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8173..9825)
                     /gene="hcp"
                     /locus_tag="ACHVC7_002997"
                     /gene_synonym="priS"
     CDS             complement(8173..9825)
                     /gene="hcp"
                     /locus_tag="ACHVC7_002997"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR2116983.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9969..10868)
                     /gene="lysO"
                     /locus_tag="ACHVC7_002998"
     CDS             complement(9969..10868)
                     /gene="lysO"
                     /locus_tag="ACHVC7_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR2116984.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2698 a         2920 c         2927 g         2540 t
ORIGIN      
        1 aaaccatggt tgccagtaaa atggacaaaa gagttaataa atgattattt gtatggctgt
       61 ttactatctc tgtactctat ctgtgcaagg gatatatata atatgagcgc cgggaataac
      121 gtgaaggttg ctgcggatgc tttccttgaa attattttct ctcttaagaa taaatattgc
      181 attaaactat taagcgctca ggaacgggcg tttatttacg aatttgcaag aatgatcttc
      241 gcttatatta atgacaaaag cattgagatc ttattacttt cttgttttgc ggcgtctgac
      301 caaaaagcaa tccagaggta tatacctcag tcacaagatg gggaagactt taggagtcat
      361 ttacaatata agttgcctct ttccgctcat taatgttgtt ggtgcaacat aataagacag
      421 gtaagcggta ccctataatg aaaccaatat cgggttatgc aacggataat cgtaaagact
      481 taagtgggtg tttttgacac aatgagatga tttcctaaac tgatcaaaac cttcttccca
      541 acatggcata tcagttcgat aatgctttag agatcgaagg cttaacaaac aaggagatcg
      601 atcatgcaat tttctacaac cccaactctg gaaggccaga ccatcgttga atattgcggt
      661 gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgccggtatc
      721 cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag
      781 atcgcctttg aggaattagg ctcccaggcg cgggcgctgg gggccgatgc cgtcgtcggt
      841 attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc
      901 ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt
      961 tattggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata
     1021 cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg
     1081 cagatgattt tgatagctcg ctggcgacac tgaccgataa gcaggtcagc tcgcattatc
     1141 tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg
     1201 aacaagaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg
     1261 acacctctat tggcattgag ctggaaaacc gtggctggca aaaatcggcc ggagtgaaat
     1321 attttgcccc gtttgaaccg acacagattc aggcgcttat tccactggcg aaagatatta
     1381 ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc
     1441 gcaaagacga tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg
     1501 cctggccgga tgcgcagcgg gttaactttt accttgccgg gcgcgcgccg cacacaccgg
     1561 tagataccgc gtcattgctg gagcttttgg cgcgctacgg ttatgacgtt aaacctgata
     1621 tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt
     1681 tatataacgg cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat
     1741 acgggcagga ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga
     1801 taccttcatc tagggtgatg atcggctgat aacctaactc ttcctgcgca cgcgtaatat
     1861 ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc ggctcttttg
     1921 ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtagggga
     1981 cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca
     2041 gtgtgcgatg ctcgccgttg gtgatattgt acacacgccc ggaaggtagc ttatcgcagg
     2101 cttcctggct tgccagccac attgcgtgca cggtattttc atagtaggtc atatcgacca
     2161 gcgcactgcc gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac
     2221 ggggaataaa gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc
     2281 gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc
     2341 gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatatcg cgatggtggt
     2401 gataatcgaa gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga
     2461 cagcccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg
     2521 tcccccaggg tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca
     2581 ctttagcttg tgatgaaacc agctcggtca gatccgccgg aacaaactct gcgcccattt
     2641 tttccagcaa tttgcccatt gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct
     2701 ggcataaaaa ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct
     2761 tcatatcaat ccactgttgt tgagaaaatg acgtgcgcat tcttccgtga tttcccccat
     2821 gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc
     2881 tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat
     2941 caataaccag taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat
     3001 agtacgatag tcgcctttat cttccaggct aaaacacagt cgtcccagcc ccgacgcttt
     3061 catgccaaat aacaacgtaa gttgtttttc cggttcaacg acaatcactt tccagctatc
     3121 caccgcatcg ccagtctgta aatattcgcg ctccgggcgg cctttcgcca gcttatgacc
     3181 gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc
     3241 tttaccgccg atttggttca ctacctgcca taaagcagca aggctggcgg acgttttaac
     3301 ggtaaacccc gcctgtttgg caaaataacc gtactccggt cgccagcggg caaaggcctg
     3361 agcgtcgtag ccccagtcgc tggagttgac cagtttttcc tcctctttca acgtgctacg
     3421 taccgcgtca tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc
     3481 cgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga
     3541 agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa
     3601 ccagcggcgc ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac
     3661 ctctggtccg gcggcttcga agatgcggtg ttcgctggcc ggatgatcta acaacgccac
     3721 cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg
     3781 cggcgttaac actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc
     3841 tgcgccaacg ataattccgg cccgaagttc ggtcacaggt acattcgctt cacgaagaat
     3901 gtccgccgta gcctgacgag cacgcagatg atccgactgc tcatgtggcg gggcctgcaa
     3961 cgaactgaga aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag
     4021 agccacctgg cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa
     4081 atagacggta tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac
     4141 tttatggcaa ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc
     4201 cgccaggatc tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc
     4261 actggcaccg agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg
     4321 cgcgccagtg ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca
     4381 ggcggacaat cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg
     4441 cggcagcatt ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat
     4501 ccgcgcctgc ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca
     4561 accgtgcgac gttatttttc agggcataca tcccggcggc agccagaatc ccggactgac
     4621 gcatcccgcc accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac
     4681 cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat
     4741 cacaatattg cgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc
     4801 gcgcaccgtc aacatgcagc gccagattgc gcttgcgggt aaattcccat gcttctttca
     4861 ggtattcacg cggcagcact ttgccgttgt gggtgttttc tagactgagt aatttggtgc
     4921 gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc
     4981 cgtcggcagc cgcgtcgatg ggttgcggtt gaatactgcc cagcaccgct gcgccaccgg
     5041 cttcaaacag ataattatgc gcggcctgac cgacaatata ctcttcgccg cgttcgcagt
     5101 gactgagcag agcgaccagg ttagcctggg tgccggtcgg cagaaaaatg gcggcttctt
     5161 taccggaaag ctctgcggcg tagtcctgca gagcattaac ggtagggtcg tctccgtaaa
     5221 cgtcgtcccc aaccggggcg gccatcattg cttcgagcat ggcgcggctc ggtcgggtaa
     5281 cggtatcact gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt
     5341 tttaccttag ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgatga
     5401 ttgcgcgcaa catatacaag ctaaatcctt tggcctgttc gagtttgatc tgcggtggaa
     5461 tggctaactc ttctttggcg accaccacat ccaccagcac cggaccgtcg atggagaagg
     5521 cgcgttgcag ggcttcatca acttcagacg ctttttctac acggataccc gtaatgccgc
     5581 acgcttcggc aatgcgggca aagtttgtgt cgtgtagttc ggtgccgtca gtcaaatagc
     5641 caccagcttt catctccatc gccacaaagc ccagcacgct gttgttaaag acgacaattt
     5701 tcactggcag tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc
     5761 catcgccgca catggcgacc acctgacgtt ctggctctgt cgcctgcgca cccagcgcct
     5821 gcggcatggc gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt
     5881 tcatttttag ataacgtgcc gcccacaccg ttggcgtacc aacgtcacag gtgaaaatag
     5941 cgtcatcggc ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct
     6001 cgctcggttt agctaaatcg tccagccctt tgcgggcgtc gcggtaatct tccagcgctt
     6061 tatccagaaa cttgcgatcg gctttttctt ccaccaatgg aagcaatgca cgcagagtcg
     6121 acttgatatc gccgaccagt gccatatcca ccttgctgtg agcgccgatg ctggctgggt
     6181 tgatatcaat ctgaatgatt ttggcatcgg tcgggtagaa ggcgcggtag ggaaattgcg
     6241 tgccgagtag cactaacgtg tcggcgttca tcatggtatg gaaacctgac gagaagccga
     6301 ttaacccggt cattccaaca tcatacggat tatcgtattc gacatgttct ttaccgcgca
     6361 gggcatgaac aataggcgct ttaattttcc cggcaaactc aactaactct ttatgcgccc
     6421 ccgcgcagcc gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca
     6481 gtttgcgtaa ctcttcttct tccggcgtca cgactggttg tggcgcatga taccagtgcg
     6541 tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc tggtaacacg acaaccgaaa
     6601 cgccacggtt aagcaccgct ttgcgcatgg caatcgccag tacttgtggg atctgctccg
     6661 ggctggaaac cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt
     6721 cctggaaata gccgctgcca atttcgctgg agggaatatg agcggcaatc gccagtaccg
     6781 gaacgtgatt gcggtggcaa tcgaacaggc cgttgattaa gtgcaggttg ccggggccgc
     6841 acgatccggc gcagaccgcc agttctccgc taagttgtgc ttcagcgcca gcggcaaagg
     6901 ccgccacttc ttcgtggcgg gtggacatcc actcgatggt gcccatgcga ttaagactgt
     6961 cactaagacc gttcagagag tcgcctgtga ctccccagat gcgtttcacc cctgccgatt
     7021 cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg
     7081 tgataacggt aacaagttta gttcatctga cggaggggga agggatggga gagaaaggag
     7141 gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg ggcataaggc gattatgcga
     7201 gaaccaaatc cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg
     7261 cgtcggtcag cgtcattgta ctgctcaccg tatattcacc ggaaaccact tttgtcttac
     7321 agcagccgca aacacccgca cggcaggcag cgacaaccgg aacgttattg ctttccagcg
     7381 cctccagtag cgtggtgcca accggggcgt aaaattctcg tgccggttgc agtttggtga
     7441 atttcagacc gctggtcgcc gcttctgcta ctggggtgaa gaatttctct ttaaagaaac
     7501 gcgtcacgcc gagcgctttc acttcctgct ctacccaatc catatacgga gccgggccgc
     7561 aggtcatcac ggtacgtgaa gctaagtcag gtacacctgc cagcagttcg cgagtgagac
     7621 gaccagcgat aaagccttcg gtaacgttat tttctgccac cagcgttacc ggatagttac
     7681 gccactcatc ggcgaaaata acatcctgcg gcgtacgcac gttgtagatc acccgcacat
     7741 cggcctgtgg acggttcttc gcaagccagc gacgcatcga cataatcggc gtgacgccgc
     7801 agcctgccgc cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca
     7861 tcgcgtccga aagccagaga taatcaccgc gttttacatc gcgcgtcagc cactgggagc
     7921 cgacaccgtc atcaatccgc cgcacggtca gggtgatata ttcactcacg cctggcgtgg
     7981 aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg cacgctgacc agtgcatatt
     8041 gcccggcgcg atatgggtag taatcgtggc aaatcaggga aatcgtccac acatccggcg
     8101 tttcttgcgt aatgtgatga acctgcatcc gccacgggca ttgattcgtt ggcatcgtca
     8161 tcgacaaact ccttacgcgc tcaacagttg cttcatgtct tcttcaacag tggtgataga
     8221 acgcaggccg aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc
     8281 agtcgggccg gtgacgatat ttttcacgcc cagagaaagc agcgtcagca gaatgacgat
     8341 cgctttctgt tcaaaccagg agagcaccag cgacagcggc agatcgttca caccacagcc
     8401 cagtttctct gccagagtga cagccagaat aatcgctgag taagcatcgt tacattgacc
     8461 tgcatctacc agacgcggca gaccttcgat atcgccaaac tcaagtttgt taaagcgata
     8521 tttaccacag gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt
     8581 gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca agcaggaaga tatgacgcag
     8641 tttttcacgg ctcaccagat caatcagcgt atcagcagcg ccaagcagcg tctggcgacc
     8701 aaaacccacg gtgataaggt gcggaatttc gctgtacggg aagcctgcca tctgttgcgc
     8761 ctgggtgata accgcagaga aatcatcacc gtccagatga cgcacgccag gccagccaac
     8821 aatgctgcgg gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt
     8881 cgaggtcatc acgatggggc cagggaaacg agcgaactcc acttgctgat tctgccagcc
     8941 gctgccgtag ttaccgacca gatgcttgaa tttacgcagc tccgggtagc catgcgcagg
     9001 cagcatttcg ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt
     9061 gtagagatct ttgagatcgt gaccggaaat cagaatgcat ttacccgccg tcgctttgac
     9121 gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct
     9181 catcactttg aagttcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc
     9241 ggcaggccag gtccccagcc acgccatgat tttatggtac tgggcataaa tatcgttgtc
     9301 gtattgaccg agaacatgcg cgtgttccat ataggccgcc gcacctttca ggccatacag
     9361 gcacagcaga cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt
     9421 aaattctgct gcctgacgtt gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc
     9481 catcgggtta tcgacgcgcg cgttggcatc tacagccagg cattgtgctt tcagcgcctc
     9541 gcgcaaggca atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt
     9601 ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc
     9661 gtattcacac gcttttaccg cccaggcaga aagcccttgc agcgccgcga tgagtaaatc
     9721 ctgaaggtca gaagtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt
     9781 tcctgccgga gtacggatag tttgttcaca ttgcacacaa aacatgatca caccttttaa
     9841 agttatattt aatatacatg tttaaggtta agacgcttaa cgcggggata aaagggattt
     9901 ttcatgcaac tttaagggag attgatttag cgcaattttg gcggcaggga tctaccgcca
     9961 gagaggtatt acgcagagaa aaaggcgatg aggatcggca ctaacaggct aagaataaaa
    10021 ccgtgaacaa ttgccgccgg gaccatatcc agcccgccag tacgttgaag aacgggcagg
    10081 gtgaaatcca ttgatgtggc accgcataag cccagtgcag tagagcggct gcggcgaatc
    10141 agcccaggga tcaacataat agcaatcagt tcacgggcca gatcattaaa aaacgccgcg
    10201 ctcccgatta ccggaccaaa agattcggtc aataaaatac cggaaagaga ataccagccg
    10261 aaaccggagg ccattgccag cgcggtattg atggggagat caagaataaa ggcgttaatt
    10321 aaaccaccaa ttaatgaact gacaaccacc accacggcga caatcattcc ccggcgatta
    10381 aggacaatct gctttaaggt catgccatta ttgcgcaact gaataccaac gaggaaaagt
    10441 agcaaaatta acgtgtattc actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc
    10501 agaccaatgg caaaaccaat cactactacg ccgcacagtt ttagcgactc cagcgccatc
    10561 gcaatacgcg acgggagttt ttcttgctga tggtggttgc gccacggcag gcctcgctcc
    10621 agccacatca gggcggcaat attacacagt aaaataacgg taatactgac ggcagaataa
    10681 tgcagaatcg ccaacaggtt actggcgagg ttatcgagaa acgccagact gatacccata
    10741 aaaaagagaa taaggtaaac catccagctt aatagctgat taataacttt taacgcagct
    10801 tgttggcgaa gcggaatgag gtaacctaca atcaggggaa ccagaatgat taacagccca
    10861 gaaaacatga aaacccagtc cttgcaaaga tgaagtcgaa atgcgcgatg acacactact
    10921 gaaagcggaa ggacgagtaa agttgcaatt aaaaggaaat gttatgcata aggagcagta
    10981 gagtattcgt tttcatttaa agatattctt gcgctttaat tacaaactgc accgatgttg
    11041 gtggcgtcaa aatcgccgag gcgttccctg aaggccgggg cagcc
//
LOCUS       DAZPLM010000138        11056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPLM010000138 DAZPLM010000000
VERSION     DAZPLM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.138"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHVC7_002999"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHVC7_002999"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR2116985.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHVC7_003000"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHVC7_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR2116986.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNVQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHVC7_003001"
     CDS             complement(3743..4162)
                     /locus_tag="ACHVC7_003001"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR2116987.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHVC7_003002"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHVC7_003002"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR2116988.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHVC7_003003"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHVC7_003003"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR2116989.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHVC7_003004"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHVC7_003004"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR2116990.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHVC7_003005"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHVC7_003005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR2116991.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHVC7_003006"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHVC7_003006"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR2116992.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHVC7_003007"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHVC7_003007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR2116993.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHVC7_003008"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHVC7_003008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR2116994.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHVC7_003009"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHVC7_003009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR2116995.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2646 a         3012 c         2732 g         2666 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgaaca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggtt
//
LOCUS       DAZPLM010000139        10832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPLM010000139 DAZPLM010000000
VERSION     DAZPLM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.139"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHVC7_003010"
     CDS             complement(<1..277)
                     /locus_tag="ACHVC7_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2116996.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="ACHVC7_003011"
     CDS             complement(444..1184)
                     /locus_tag="ACHVC7_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR2116997.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHVC7_003012"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHVC7_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR2116998.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHVC7_003013"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHVC7_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR2116999.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHVC7_003014"
     CDS             complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHVC7_003014"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR2117000.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHVC7_003015"
     CDS             complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHVC7_003015"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR2117001.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHVC7_003016"
     CDS             complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHVC7_003016"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR2117002.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7000..7431
                     /locus_tag="ACHVC7_003017"
     CDS             7000..7431
                     /locus_tag="ACHVC7_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR2117003.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7454..8032
                     /locus_tag="ACHVC7_003018"
     CDS             7454..8032
                     /locus_tag="ACHVC7_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR2117004.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8033..8740
                     /locus_tag="ACHVC7_003019"
     CDS             8033..8740
                     /locus_tag="ACHVC7_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR2117005.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8728..9405
                     /locus_tag="ACHVC7_003020"
     CDS             8728..9405
                     /locus_tag="ACHVC7_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2117006.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9399..10076
                     /locus_tag="ACHVC7_003021"
     CDS             9399..10076
                     /locus_tag="ACHVC7_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2117007.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10048..10761)
                     /locus_tag="ACHVC7_003022"
     CDS             complement(10048..10761)
                     /locus_tag="ACHVC7_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR2117008.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10758..>10832)
                     /locus_tag="ACHVC7_003023"
     CDS             complement(10758..>10832)
                     /locus_tag="ACHVC7_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2117009.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2878 c         2699 g         2675 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     3061 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
     3121 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     3181 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac
     3241 tgcccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     3301 cattgggtat cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt
     3361 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     3421 ttgtgtttct tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     3481 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     3541 ctgattttgc tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     3601 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     3661 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
     3721 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     3781 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     3841 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     3901 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     3961 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     4021 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     4081 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     4141 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
     4201 cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     4261 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
     4321 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
     4381 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
     4441 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
     4501 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
     4561 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
     4621 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
     4681 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
     4741 agatttcagg gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
     4801 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
     4861 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
     4921 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
     4981 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
     5041 cgcgtccagt tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat
     5101 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
     5161 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
     5221 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
     5281 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
     5341 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
     5401 tttcgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
     5461 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
     5521 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
     5581 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
     5641 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
     5701 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
     5761 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
     5821 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
     5881 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
     5941 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
     6001 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
     6061 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
     6121 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
     6181 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga
     6241 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
     6301 cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
     6361 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
     6421 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
     6481 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
     6541 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
     6601 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
     6661 tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
     6721 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
     6781 gtggaataaa aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt
     6841 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
     6901 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
     6961 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
     7021 tattcttccc ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc
     7081 cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc
     7141 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac
     7201 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
     7261 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
     7321 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa
     7381 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
     7441 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
     7501 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
     7561 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
     7621 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
     7681 tgcacccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
     7741 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
     7801 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
     7861 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
     7921 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
     7981 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
     8041 atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca
     8101 gaccattttg ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac
     8161 gcggcggcgg gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct
     8221 ggtacacggt ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg
     8281 gacgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
     8341 gatgcagtac gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc
     8401 aggcgtcgct tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt
     8461 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
     8521 gcgtttacgt gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct
     8581 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
     8641 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
     8701 gatgctaaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
     8761 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
     8821 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
     8881 tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt
     8941 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
     9001 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
     9061 ctgtgtcttg ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag
     9121 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg
     9181 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt
     9241 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
     9301 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
     9361 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
     9421 cttatcgctg gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac
     9481 aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
     9541 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
     9601 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
     9661 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
     9721 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
     9781 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
     9841 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
     9901 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
     9961 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    10021 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    10081 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    10141 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    10201 ccccaccaga cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca
    10261 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    10321 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    10381 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    10441 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    10501 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    10561 cacgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    10621 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    10681 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    10741 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    10801 agcgccgttt tgaccattcc gctaaaacgc gc
//
LOCUS       DAZPLM010000140        10810 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPLM010000140 DAZPLM010000000
VERSION     DAZPLM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.140"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..694)
                     /locus_tag="ACHVC7_003024"
     CDS             complement(<1..694)
                     /locus_tag="ACHVC7_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2117010.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRA"
     gene            950..1105
                     /gene="rseD"
                     /locus_tag="ACHVC7_003025"
     CDS             950..1105
                     /gene="rseD"
                     /locus_tag="ACHVC7_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR2117011.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1102..1677
                     /gene="rpoE"
                     /locus_tag="ACHVC7_003026"
     CDS             1102..1677
                     /gene="rpoE"
                     /locus_tag="ACHVC7_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR2117012.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1710..2360
                     /gene="rseA"
                     /locus_tag="ACHVC7_003027"
     CDS             1710..2360
                     /gene="rseA"
                     /locus_tag="ACHVC7_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR2117013.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2360..3316
                     /gene="rseB"
                     /locus_tag="ACHVC7_003028"
     CDS             2360..3316
                     /gene="rseB"
                     /locus_tag="ACHVC7_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR2117014.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3313..3792
                     /gene="rseC"
                     /locus_tag="ACHVC7_003029"
     CDS             3313..3792
                     /gene="rseC"
                     /locus_tag="ACHVC7_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR2117015.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3990..5789
                     /gene="lepA"
                     /locus_tag="ACHVC7_003030"
     CDS             3990..5789
                     /gene="lepA"
                     /locus_tag="ACHVC7_003030"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR2117016.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5805..6779
                     /gene="lepB"
                     /locus_tag="ACHVC7_003031"
     CDS             5805..6779
                     /gene="lepB"
                     /locus_tag="ACHVC7_003031"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR2117017.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7052..7732
                     /gene="rnc"
                     /locus_tag="ACHVC7_003032"
     CDS             7052..7732
                     /gene="rnc"
                     /locus_tag="ACHVC7_003032"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR2117018.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7729..8634
                     /gene="era"
                     /locus_tag="ACHVC7_003033"
     CDS             7729..8634
                     /gene="era"
                     /locus_tag="ACHVC7_003033"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR2117019.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8646..9374
                     /gene="recO"
                     /locus_tag="ACHVC7_003034"
     CDS             8646..9374
                     /gene="recO"
                     /locus_tag="ACHVC7_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR2117020.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9386..10117
                     /gene="pdxJ"
                     /locus_tag="ACHVC7_003035"
     CDS             9386..10117
                     /gene="pdxJ"
                     /locus_tag="ACHVC7_003035"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR2117021.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10117..10497
                     /gene="acpS"
                     /locus_tag="ACHVC7_003036"
     CDS             10117..10497
                     /gene="acpS"
                     /locus_tag="ACHVC7_003036"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR2117022.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10719..10799
                     /gene="shoB"
                     /locus_tag="ACHVC7_003037"
     CDS             10719..10799
                     /gene="shoB"
                     /locus_tag="ACHVC7_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR2117023.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2715 a         2660 c         2877 g         2558 t
ORIGIN      
        1 ccgcgcgcca cgccatagca atgccatcgc cagaagaaat atccggattg gtggtgtact
       61 gatacacctt cgacgcaccg ccggttgcca gtaccactgc ttttgcgtgg caggtttcca
      121 ccgtttcttt atttcggttc catacccacg cgcctacaac ccgtcgcgtg cccggcaggc
      181 caattttgtc agagacaatc agatcaactg cgttgctgcg ctccagcaca cgaatattcg
      241 gatggttctg cgccttgctc accagcgtgg attgtacttc tctaccggtg gcgtcggcgg
      301 catgaagaat acgacggtga ctatgtccac cttcacgggt cagatggtag ctttcttcgc
      361 cattcggttg aacgtgggta tcaaacaaaa ccccctggtc aattaaccat tgcacacagg
      421 aacgagcatt gctggcgaca aattcaactg catggcgatc gcaaatacca gccccggcaa
      481 tcaatgtgtc ttccacatgc gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac
      541 cgccctgggc ataaaatgtt gaaccttccg ttaccgggcc tttacttaga acgatgacct
      601 gatgctggtc agccaggcgt agcgccagtg aaagtccggc tgcgccgcta ccaataatca
      661 acacgtcaca tgaatgttca gggagagtat tcattttctt tgtttaattt actaaacatt
      721 gtttggtcag catagcatca tgttgtgcga ataaacacct gctattttaa tatttgttac
      781 agtggctaaa cacgttgacg cagggcggcg agaaaaagag aagttactgg ctggtggagg
      841 attaggtggt gaaataaaaa ggccgttggg ttactcttca ggcagttaaa tgggcatttc
      901 tacacagata atgcgatgtt cagattctgt agacttataa tgatagataa tgatccgtct
      961 acagcatgac aaacaaaaac agatgcgtta cggaacttta caaaaacgag acactctaac
     1021 cctttgcttg ctcaaattgc agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg
     1081 tttggggaga ctttacctcg gatgagcgag cagttaacgg accaggtcct ggttgaacgg
     1141 gtccagaagg gagatcagaa agcctttaac ttactggtag tgcgctatca gcataaagtg
     1201 gcgagtctgg tttcccgcta tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct
     1261 tttattaaag cctatcgtgc gctggattcg ttccggggag atagcgcttt ttatacatgg
     1321 ctgtatcgga ttgctgtaaa tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca
     1381 ccttccagtg atgtggatgc cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa
     1441 gaaatttcga accctgagaa cttaatgttg tcagaagaac tgagacagat agttttccga
     1501 actattgagt ccctcccgga agatttgcgc atggcaataa ccttgcggga gctggatggc
     1561 ctgagctatg aagagatagc cgctatcatg gattgtccgg taggtacggt gcgttcacgt
     1621 atcttccgag cgagggaagc tattgataac aaagttcaac cgcttatcag gcgttgacga
     1681 tagcgggata ctggataagg gtattaggca tgcagaaaga acaactttcc gctttaatgg
     1741 atggcgaaac gctggatagt gagctgctta acgaactggc tcataaccca gaaatgcaga
     1801 aaacctggga aagctatcac ttaatccgtg attcaatgcg gggtgatact cccgaggtgc
     1861 tccatttcga tatctcttca cgcgtgatgg ccgccattga agaagagcca gtacgtcaac
     1921 cggcgacatt gatcccggaa gcccagcctg cgccgcatca atggcacaaa atgccattct
     1981 ggcagaaagt acgtccgtgg gcggcacagc ttacccaaat gggcgtagca gcatgcgtat
     2041 cgcttgcagt tatcgttggc gtccagcact ataatggaca atctgaaacg tcccagcagc
     2101 ccgaaacgcc ggtatttaat acactgccga tgatgggtaa agccagcccg gtaagcctgg
     2161 gagtaccttc tgaagcgacc gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc
     2221 gtcgcattaa tgcaatgttg caggattacg aactgcaacg ccgactccac tctgaacagc
     2281 ttcagtttga gcaggcgcaa acccagcaag ccgctgtaca ggtgccagga cttcaaactt
     2341 taggaacgca atcgcagtaa tgaagcaact ttggtttgcc atgtcattag tgacaggtag
     2401 cctgttattc tctgctaacg cctcggccac tcccgcgtcc ggggcgttat tacagcagat
     2461 gaacctggcc agtcagtcac tgaattacga gctgtcattc atcagcatca ataaacaggg
     2521 tgttgagtct ctgcgttatc gacatgcacg cctcgataac cgtcctcttg cacaattgtt
     2581 gcaaatggat ggcccgcgcc gggaagtggt acagcgcggc aatgaaatca gctattttga
     2641 accgggactt gaaccgttca cgcttaatgg cgattacatt gttgattctc tgccatcgct
     2701 tatctatacc gatttcaaac gcctttctcc ttactacgac tttatctccg tcgggcgcac
     2761 gcgtattgct gatcgtcttt gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta
     2821 cagctacatc gtgtggatgg acactgaatc gaaattaccg atgagggttg atctccttga
     2881 tcgcgatggt gaaacgctgg aacaatttcg cgtgatttct tttaacgtca atcaggatat
     2941 cagcagcagt atgcagacgc tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt
     3001 aggtgaaaaa gctaaattca gctggacgcc aacctggttg ccacagggtt ttagcgaagt
     3061 ttccagtagt cgacgtccgc taccgacgat ggacaacatg cctatcgaat cacgtctcta
     3121 ttccgacgga ttattcagct tctcggtaaa cgttaaccgc gctacgccat cgagcaccga
     3181 tcagatgttg cgcaccggac gcagaaccgt cagtacaagc gtacgtgata acgccgaaat
     3241 caccattgtc ggtgaactgc cgccgcaaac ggcgaaacgc attgccgaga atattaagtt
     3301 cggggcagcg caatgatcaa agagtgggct accgtcgtct cctggcaaaa cgggcaggcg
     3361 ctggtcagtt gtgatgttaa agcctcatgc agcagctgcg cttcacgcgc cggttgcggt
     3421 agccgcgtgt tgaataaact tggcccgcaa accacgcata ccattgtcgt accctgtgat
     3481 gaaccgttag tgccggggca aaaagtggaa ttggggatcg ccgaaggcag cctgcttagc
     3541 tccgcattac tggtttatat gtcgccgctg gtgggattat tccttatcgc ttcgctattt
     3601 cagctactct ttgcttctga cgttgcagca ttatgtggtg cgatccttgg cggtattggc
     3661 gggtttctga ttgcgcgcgg ttactcgcgt aagtttgctg cccgggcaga atggcaaccg
     3721 atcatattaa gcgtggcatt gccgcctgac cttgtgcgat ttgaaacatc ttcggaagac
     3781 gcgagccagt gatttcactg gctttgttgc attttgcata acaaatgcga ggacgtttcc
     3841 agataccaca atccatacgg tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc
     3901 ggcgtttcta ttaatacaga cgttaagctc agaacagcga cttctaaaag cctggttaac
     3961 cgggcattaa ggcacaataa tcatacttta tgaagaatat acgtaacttt tcgatcatag
     4021 ctcacattga ccacggtaaa tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc
     4081 tgtctgaccg tgaaatggag gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg
     4141 gcattaccat caaagcgcaa agcgtgacgc tggactacaa agcgtctgac ggcgaaacct
     4201 atcagcttaa ctttatcgac accccgggcc acgtagactt ctcctatgaa gtttcccgtt
     4261 cgctggctgc ctgtgaaggt gcattgctgg tggtcgacgc cgggcagggc gtggaagcgc
     4321 aaaccctggc aaactgctac accgccatgg aaatggatct cgaagtggtg ccagtactga
     4381 acaagattga cctgccggca gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg
     4441 tcggcatcga cgccactgac gcggtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg
     4501 acgttctcga acgtctggtg cgcgacattc cgccgccgga aggcgatccg gaaggcccgt
     4561 tgcaggcact aattatcgac tcatggttcg acaactacct gggcgttgtt tcacttatcc
     4621 gtattaaaaa cggcaccctg cgtaagggcg acaaagtgaa agtcatgagt accgggcaga
     4681 cctataacgc cgaccgtctg ggcatcttca cgccgaaaca ggttgaccgc actgaactga
     4741 aatgtggcga agtaggctgg ctggtatgtg cgattaaaga tatccacggc gctccagtcg
     4801 gcgatacctt aacgctggcg cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag
     4861 tcaaaccgca ggtatacgcc ggtctgttcc cggtaagttc cgacgactat gaagccttcc
     4921 gtgacgcgct gggtaaactc agcctgaatg atgcctcact gttctatgag ccggaaagct
     4981 ccagcgcgct gggctttggt ttccgctgcg gcttccttgg cctgctgcac atggagatca
     5041 tccaggaacg tctggaacgt gaatacgatc tggatctgat caccactgcg ccgaccgtag
     5101 tgtatgaagt tgaaaccacg tcaagggaag ttatctacgt cgacagccca tccaagctgc
     5161 ctgcggtaaa taacatttac gaactgcgcg agccgattgc agagtgtcac atgctgctgc
     5221 cgcaggcata tctcggcaac gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca
     5281 atatggttta ccacggtaat caggtggcgc tgacgtacga gatcccgatg gcggaagtgg
     5341 tgctcgactt cttcgatcgc ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca
     5401 acttcaaacg cttccaggcg tccgacatgg tacgtgtaga cgtattaatc aacggtgaac
     5461 gtgttgatgc gttggcgctg atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt
     5521 tggtggagaa gatgaaagat ctgatcccac gccagcagtt tgatatcgcc attcaggcag
     5581 cgattggtac gcacattatt gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg
     5641 ctaaatgtta tggcggcgat atcagccgta agaaaaagct gctgcagaag cagaaagaag
     5701 gtaagaaacg catgaagcag atcggtaacg tcgagctgcc gcaggaagcg ttcctcgcca
     5761 ttctgcacgt cggcaaagac aacaaataac ccttaggagt tggcatggcg aatatgtttg
     5821 ccctgattct ggtgattgcc acactggtga cgggcatttt atggtgcgtg gataaattct
     5881 ttttcgcacc taaacggcgg gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac
     5941 tggataaagc aacgttgaaa aaggttgcac cgaagcctgg ctggctggaa accggagctt
     6001 ctgtttttcc ggtgctggct atcgtattga ttgtacgttc gtttatttat gaaccgttcc
     6061 agatcccgtc aggttcgatg atgccgactc tgttaatcgg tgattttatt ctggtagaga
     6121 agtttgctta tggcattaaa gatcctatct accagaaaac gctgatcgaa accggtcatc
     6181 cgaaacgcgg cgatatcgtg gtctttaaat atccggaaga tccaaagctt gattacatca
     6241 agcgcgcggt gggtttaccg ggcgataaag tcacttacga tccggtctca aaagagctga
     6301 cgattcaacc gggatgcagt tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact
     6361 caaacgtgga accgagcgat ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga
     6421 ccagcggatt ctttgaagtg ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg
     6481 agcgtaaaga gacactgggt gatgtgacgc accgaattct gacagtgccg attgcgcagg
     6541 accaggtggg gatgtattac cagcagccag ggcaacaact ggcaacctgg attgttccgc
     6601 cgggacaata cttcatgatg ggcgacaacc gcgacaacag cgcggacagc cgttactggg
     6661 gctttgtgcc tgaagcgaat ctggtcggtc gggcaacggc tatctggatg agtttcgata
     6721 agcaagaagg cgaatggccg acaggtgtgc gcttaagtcg cattggcggc atccattaat
     6781 agcccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt tatgagataa
     6841 atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc cgaagtttaa
     6901 ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat ataccggtaa
     6961 actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt tcgtgtgctg
     7021 aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta atcggcttca
     7081 acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat taactcatcg
     7141 tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta ttctgagcta
     7201 cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg atatgagccg
     7261 gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg aatttgagtt
     7321 aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc gtcgtgagtc
     7381 aattctcgcc gataccgtcg aagcattaat tggtggcgta ttcctcgaca gtgatattca
     7441 aaccgtcgag aaattaatcc tcaactggta tcaaactcgt ttggacgaaa ttagcccagg
     7501 cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc gccatctgcc
     7561 gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg aatttactat
     7621 ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa gccgtcgtaa
     7681 ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat gagcatcgat
     7741 aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa atccacattg
     7801 ttgaacaaac tgctggggca gaagatctcc atcacctccc gcaaagcgca aacaactcgt
     7861 caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt tgatacccct
     7921 ggcctgcata tggaagaaaa acgcgctatt aaccgcctga tgaacaaagc ggcgagcagc
     7981 tctattggcg acgttgagct ggtgattttt gtcgttgaag gcacccgctg gacgccggac
     8041 gacgaaatgg tgctcaacaa actgcgcgat ggcaaagcgc cggtgatcct cgcggtgaac
     8101 aaagtggata acgtgcagga aaaagccgat ctgctgccgc acttacagtt cctggcaagt
     8161 cagatgaact tcctcgatat cgtgccgatc tctgctgaaa ccgggctgaa tgtcgacact
     8221 atcgcagcaa tcgtgcgtaa gcatctgcct gaagcgaccc atcacttccc ggaagattac
     8281 atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa actgatgcgt
     8341 ttcctcggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt cgtctctaac
     8401 gaacgcggtg gttatgacat caatggtttg attctcgttg agcgtgaagg gcagaagaag
     8461 atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc gcgtaaagac
     8521 atgcaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt gaaatccggt
     8581 tgggccgacg acgaacgcgc actgcgcagt ctcggttacg ttgacgatct ttaagagtca
     8641 ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg agcgaaacca
     8701 gcctgatgct ggacgtcttc acggaggaat cgggtcgcgt gcgtctggtt gccaaaggcg
     8761 cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct ctcttgctac
     8821 gtttcggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc tcgctggcgc
     8881 tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt ctctcccgcg
     8941 tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac tgcattcagt
     9001 ctcttgcagg tgccactggt acgccagaac ccgcgctgcg ccgctttgaa ctggcacttc
     9061 tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc gagccggtag
     9121 atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc gtcgttatcg
     9181 acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa tttcctgacg
     9241 cagacacact acgcgccgcg aaacgcttta cccgcatggc gcttaagccg tatcttggcg
     9301 gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga acggtgaaaa
     9361 cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca acattgacca
     9421 tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc aggcggcgtt
     9481 tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag atcgccgcca
     9541 cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa tgaatctgga
     9601 gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac atttttgctg
     9661 cctggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg ttgcagggca
     9721 gcgtgaaaaa atgcgcgatg cctgcaaacg tctgacagat gccgggattc agatttctct
     9781 ctttatcgac gccgatgaag agcagatcaa agctgcggca gaggttggcg caccgtttat
     9841 cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg cgcaagagct
     9901 ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag ttaacgccgg
     9961 acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga tgcatgaact
    10021 gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag atgcggtggc
    10081 agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg tttaggcacg
    10141 gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga gcgcctggca
    10201 cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca gccggtgcgt
    10261 tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg caccgggatc
    10321 cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg caaaccacgg
    10381 ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt aaatatgcat
    10441 gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga aagttaaaat
    10501 aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg gttttacttt
    10561 ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat cagtgtactt
    10621 atcttttaca agcaacttgc aatctttagc ataaaaactc aagcctttac gaagaaagca
    10681 atactgaacg gctgcattcc ttgtttaagg aaaaacgaat gactgattgc cgatacctga
    10741 ttaaacgggt catcaaaatc atcattgctg ttttacagct gatccttctg ttcttataac
    10801 acaaggaaac
//
LOCUS       DAZPLM010000141        10687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPLM010000141 DAZPLM010000000
VERSION     DAZPLM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.141"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHVC7_003038"
     CDS             complement(<1..784)
                     /locus_tag="ACHVC7_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR2117024.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHVC7_003039"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHVC7_003039"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR2117025.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHVC7_003040"
     CDS             1860..2747
                     /locus_tag="ACHVC7_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR2117026.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHVC7_003041"
     CDS             2780..3790
                     /locus_tag="ACHVC7_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR2117027.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHVC7_003042"
     CDS             3860..5293
                     /locus_tag="ACHVC7_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2117028.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHVC7_003043"
     CDS             5392..6741
                     /locus_tag="ACHVC7_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2117029.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHVC7_003044"
     CDS             6731..7618
                     /locus_tag="ACHVC7_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR2117030.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHVC7_003045"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHVC7_003045"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR2117031.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHVC7_003046"
     CDS             8352..9224
                     /locus_tag="ACHVC7_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR2117032.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHVC7_003047"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHVC7_003047"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR2117033.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHVC7_003048"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHVC7_003048"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR2117034.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2531 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZPLM010000142        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPLM010000142 DAZPLM010000000
VERSION     DAZPLM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.142"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHVC7_003049"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHVC7_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR2117035.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHVC7_003050"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHVC7_003050"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR2117036.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHVC7_003051"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHVC7_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR2117037.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHVC7_003052"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHVC7_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR2117038.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHVC7_003053"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHVC7_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHVC7_003054"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHVC7_003054"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR2117039.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHVC7_003055"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHVC7_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR2117040.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHVC7_003056"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHVC7_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR2117041.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLVVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHVC7_003057"
     CDS             complement(9457..10149)
                     /locus_tag="ACHVC7_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR2117042.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2557 a         2522 c         2762 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccacc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZPLM010000143        10443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPLM010000143 DAZPLM010000000
VERSION     DAZPLM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.143"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            109..684
                     /gene="ssuE"
                     /locus_tag="ACHVC7_003058"
     CDS             109..684
                     /gene="ssuE"
                     /locus_tag="ACHVC7_003058"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR2117043.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            677..1636
                     /gene="ssuA"
                     /locus_tag="ACHVC7_003059"
     CDS             677..1636
                     /gene="ssuA"
                     /locus_tag="ACHVC7_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR2117044.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1633..2780
                     /gene="ssuD"
                     /locus_tag="ACHVC7_003060"
                     /pseudo
     CDS             1633..2780
                     /gene="ssuD"
                     /locus_tag="ACHVC7_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2792..3583
                     /gene="ssuC"
                     /locus_tag="ACHVC7_003061"
     CDS             2792..3583
                     /gene="ssuC"
                     /locus_tag="ACHVC7_003061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR2117045.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3580..4347
                     /gene="ssuB"
                     /locus_tag="ACHVC7_003062"
     CDS             3580..4347
                     /gene="ssuB"
                     /locus_tag="ACHVC7_003062"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR2117046.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4554..7166)
                     /gene="pepN"
                     /locus_tag="ACHVC7_003063"
     CDS             complement(4554..7166)
                     /gene="pepN"
                     /locus_tag="ACHVC7_003063"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR2117047.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7432..8634
                     /gene="pncB"
                     /locus_tag="ACHVC7_003064"
     CDS             7432..8634
                     /gene="pncB"
                     /locus_tag="ACHVC7_003064"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR2117048.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8804..10204
                     /gene="asnS"
                     /locus_tag="ACHVC7_003065"
     CDS             8804..10204
                     /gene="asnS"
                     /locus_tag="ACHVC7_003065"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR2117049.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2353 a         2690 c         2822 g         2578 t
ORIGIN      
        1 aaaaagatac acaaaattta tatttggcat tttcttgtct ctccgacaga ctgaccgaat
       61 tgttatcaat gttaacaaaa aaagaacaat tggttataag gagagagtat gcgtgtcatc
      121 accctggcgg gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg
      181 gaaaaactaa atggcctgga tgtagaggtt tatcactgga atctgcaaaa cttcgccccg
      241 gaagatctgc tttacgctcg tttcgatagt ccggcactca agaccttcac cgaacagctg
      301 caacaggctg atggactgat agtcgctaca cctgtgtata aagccgccta ttccggcgcg
      361 ctgaaaaccc tgctcgacct gctgccagag cgcgctttgc aaggcaaagt ggtgctaccg
      421 ctggcgacgg gcggtacggt ggctcatctg ctggcggtcg attatgccct taagccagtt
      481 ttaagtgcga tgaaagccca ggagctcctg cacggcgtgt ttgccgatga ctcacaagtt
      541 attgattacc atcacaaacc ccagttcacg ccaaatctgc aaacccgtct tgataccgcg
      601 ctagaaactt tctggcaggc attgcaccgc cgcgatgttc aggttcctga ccttctgtct
      661 ctgcgaggta atgtccatgc gtaacatcat taaactggcg ctggcgggat tgcttagcgt
      721 ctctactttt gcggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa
      781 aggcagtatt ggtatggtat tggcaaaaag ccaccagtta ctggaaaaac gctatccgca
      841 atcaaaaatc tcctgggtgg agttccccgc gggtccgcaa atgttggaag cgttaaacgt
      901 tggcagtatt gatctcggca gtaccgggga tattccgcca atctttgccc aggctgccgg
      961 ggctgatttg atatatgtgg gcgtcgagcc accgaagccc aaagccgaag tgattctggt
     1021 ggcagaaaac agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag ttgcctttca
     1081 gaaaggttcc agttcacaca accttttact gcgtgcactg cgtcaggccg gacttaagtt
     1141 tactgacatc cagcccactt atctgacgcc agctgatgcc cgcgccgcgt tccagcaagg
     1201 taacgttgac gcctgggcta tctgggatcc ctactactcc gctgcgttat tacagggcgg
     1261 cgtgcgggtg ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc
     1321 tcgcccctat gcagaaaaaa acggcgcttt tattcagggc gtactggcaa cctttagtga
     1381 ggccgatgcg ttaacccgca gccagcgcga gcaaagcatc gctttactgg caaaaacgat
     1441 gggcttaccg gcaccggtga ttgcctcgta tctggatcat cgtcctccca ccaccatcaa
     1501 accggtgagc gctgaagttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa
     1561 ccgtctggtg tcgaaaaaag ttgatattcg ccagcgcatc tggcagccca ctcacctgga
     1621 aggaaaacaa ttatgagtct gaatatgttc tggtttttac cgacccatgg tgacgggcat
     1681 tatcttggaa cggaagaagg ttcacgcccg gttgatcacg gctatctgca acaaattgcg
     1741 caagcggcgg atcgtctggg ctataccggt gtgctgatcc caacggggcg atcgtgtgaa
     1801 gatgcgtggc tggtggcggc gtcgatgatc ccggtgacgc agcggctgaa gtttcttgtc
     1861 gccctgcgtc ccagcgtaac ctcacctacc gttgccgctc gccaggccgc cacgcttgac
     1921 cgtctctcaa atggtcgtgc gttgtttaat ctggtcacag gcagcgatcc acaagagttg
     1981 gcaggcgacg gcgtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc
     2041 caggtctggc ggggcgctta ttgctggggg aaagcgtcga tttcaacggt aaacatattc
     2101 atgtgcgggg agcaaaactg ctcttcccgc cgattcaaca gccgtatcct ccgctttact
     2161 ttggcggatc gtcagatgtc gcccaggagc tggcggcaga acaagttgat ctctacctca
     2221 cctggggcga accgccagag ctggtaaaag agaagatcga acaagtgcgg gcgaaagctg
     2281 ccgcgcatgg acgcaaaatt cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta
     2341 acgacgaagc gtggcaggcc gccgagcggt taatctcgca tcttgatgat gaaactatcg
     2401 ccaaagcaca ggccgcattc gcccggacgg attccgtagg acaacagcga atggcggcgc
     2461 tacataacgg caagcgcgac aatctggaga tcagccccaa tttatgggcg ggcgttggct
     2521 tagtgcgcgg cggtgccgga acggcgctgg tgggcgatgg tcctacggtc gccgcgcgaa
     2581 tcaacgaata cgccgcgctt ggcatcgaca gctttgtgct ttcgggctac ccgcatctgg
     2641 aagaagcgta tcgggttggc gagttgctgt tcccacatct ggatgtcgcc attccggaaa
     2701 ttccccagcc gcagccgctg aatccgcaag gcgaagcggt ggcgaatgat tttatccccc
     2761 gtaaagtcgc gcaaagctaa ggaacacgat gatggcaacg ccagtgaaga agtggttatt
     2821 gcgcgttgcc ccctggtttt taccggtggg catcgtggcg gtgtggcaac tggcctcctc
     2881 ggttggctgg ctgtcgacgc gtattttgcc ttcaccggaa ggagtggtga cggcgttctg
     2941 gacgctctcc gccagcggcg aattgtggca gcatctggcg atcagttcct ggcgggcgct
     3001 gattggcttt tcaattggcg gatcgctggg cttgattctg ggactgatta gcgggctgtc
     3061 acgctgggga gaacggctgc tggatacctc aattcagatg ttgcgcaacg taccgcatct
     3121 ggcgctgatc ccgctggtga ttttgtggtt tggcattgat gaatccgcaa aaatctttct
     3181 ggtggcgctc ggcacgctat tccccattta tatcaacacc tggcatggca tccgtaatat
     3241 cgatcgcggt ctggtggaaa tggcgcgtag ctatggatta tccggcatac cgctgtttat
     3301 ccatgtgatc ctgcctggtg ccctgccctc aattatggtc ggcgtgcgtt ttgcgttggg
     3361 cctgatgtgg ctgacgctga ttgttgccga aaccatttct gccaattcag gcattggtta
     3421 tctggcgatg aatgcgcggg agtttttgca aacggacgtg gtggtagtcg ctattattct
     3481 ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag ctgttagagc gcctctggtt
     3541 gcgctggaac ccggcttatc atttgaagga ggccacggta tgaatactgc tcgtctgaac
     3601 cagggcacac cattgttgct caatgcagta agcaaacatt acgcggaaaa tatcgtcctg
     3661 aaccaactgg atttacatat tccggcaggt cagtttgtgg cggtggtggg ccgcagcggt
     3721 ggtggcaaaa gtaccctgct gcgcctgctg gcaggtctgg aaacgccaac cgcaggcgat
     3781 gtgttagcgg gcaccacacc gctggctgag attcaggaag acacgcgaat gatgtttcag
     3841 gatgcgcgtc tgctgccgtg gaaatcggtg attgataacg ttgggttagg ccttaaaggc
     3901 cagtggcgcg atgccgcgcg tcgggcgttg gctgcagtag gactggagaa tcgcgccggg
     3961 gaatggcctg ccgcactttc tggcgggcag aaacagcgtg tggcgctggc aagagcgttg
     4021 attcatcgac cgggtttatt gttgcttgat gaaccgctcg gggcactgga cgccttaacg
     4081 cgactcgaga tgcaggattt gattgtgtca ctttggcagc agcacggctt taccgtactg
     4141 ttggtgacgc atgatgtgag cgaagcggta gcaatggctg accgggtgtt gttaattgaa
     4201 gagggaaaaa ttggtctgga tttgacagtg gatatccccc gtccgcgtcg attagggtcg
     4261 gtgcgattag cggagctgga agcggaagta ttgcaacggg taatgcggcg tgggcattct
     4321 gaacaactga ttcggcgtca tggttaatat gccggatgtg gcgcacaaaa ccagcatttt
     4381 caacggactt gtaggcctga taagacacgt caagcatcgc atcaggcagt tgggcacggc
     4441 tgccggatgc ggcgtgaacg ccttatccag cctacaaaac cagcaatttc aacggagttg
     4501 taggcctgat aagacgcgtc aagcgtcgca tcaggcattt tggctcaaaa gtatcaagcc
     4561 agtgctttgg tgatcttctc gtacagatcg ccagagagat tttccagccc tttcaactgt
     4621 tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat aacgtttcag gcgaatcagc
     4681 ggttcaatca gacgtgaagc tacctgcggg ttacggctgt tgaggtcggt aagcatttcc
     4741 accaggaact ggtaaccgct gccatcttcg gcatggaacg ctgccgggtt gctgcccgca
     4801 aacgcaccaa tcaacgaacg aatacggttc gggttgctca tggtaaatga gcgatgctgc
     4861 aacagaccac gcaccgtctc cagcacattc gccgccgggc tggtggcttg caggataaac
     4921 catttatcca tcaccagacc gtcctgatgc cacttatcgt cgtactcctg catcagcgcg
     4981 tcacggcaag gcagctgtgc agcaaccgcc gcagaaagcg ctgccagcgc atcggtcata
     5041 ttgtttgctt catggtattg cttgctcacc agcacgtcag ccagatgcgt ttcaccaaaa
     5101 gcaaggaagc gcaggcaggc atttcgcaga gtgcgttttg caatatcttc atgctcaaca
     5161 cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatccgc cagttcagtc
     5221 gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc gatgatatcg
     5281 aacagttcag ccatttcatt gacagaaggc agcgtcagga tttctgccgc cagcgccgga
     5341 tcaatcttct catcgagcag caccgcgcgg aaagcgtcag ccacatgtac cggcagagac
     5401 agcggctgcc cttgctgatg acgggcgacg ttcagcttga tgtaggttgc cagcaaactt
     5461 tgcgccgcat cccaacggga gaaatcatta cgcgcatgac gcatcaggaa ggtcagttgc
     5521 tgatcactcc acttatattc cagtttcact ggcgcagaga attcgcacag cagcgcaggc
     5581 accggctgga agtagacatt atcaaaaaca aaggtctgtt ccgcctgggt gacgttcagc
     5641 acggaattca ccggatgacc gcctttctgc aacgggatca ctttgccttc gttgtcatac
     5701 aattcgatgg caaacggaat atgcagcggc tgtttttctg cctgatccgg cgtggctggc
     5761 gtgcgctggc tgatggtcag ggtgtactgc tcagtttccg gattgtagtc gtctttgacg
     5821 gtcacaatcg gcgtaccgga ctggctgtac caacggcgga aatgggagag atcaacattc
     5881 gacgcatctt ccatcgcctg cacaaagtcg tcacaggtcg ctgcgctacc atcatgacgc
     5941 tcgaaataga gttgcatccc tttctggaag ttttcttcgc ccagcagggt gtggatcata
     6001 cggatcactt ccgcgccctt ctcgtaaacg gtcagggtgt agaagttgtt catctcaatg
     6061 accatgtccg ggcggattgg gtgcgccatc gggctggcgt cttccgcaaa ctgcaatccg
     6121 cgcatggtgc gcacattatt gatgcggtta actgcgcggg aaccaaggtc agagctgaat
     6181 tcctgatcgc ggaagacggt taaaccttct ttcaggctga gctggaacca gtcgcggcag
     6241 gtcactcggt taccggtcca gttatggaaa tattcatggc cgataacgcg ttcaatatcg
     6301 aggtaatctt tgtcggtggc ggtgtcggtg cgggccagca catatttgga gttaaagata
     6361 ttcagaccct tattctccat tgcgcccata ttgaagaaat ccaccgcgac gatcatatag
     6421 atgtcgaggt catactccag gccgaagcgt tcttcatccc atttcattga gtttttcagc
     6481 gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat cgacgtacag ctccagcgcc
     6541 acttcgcgac cagaacgcgt ggtaaaggtg tcgcgcagta catcaaagtc gcctgccacc
     6601 agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc actgtaccca atggcgtccg
     6661 ttttccagtt caccttgcgc aacgcggtta ccgttggaaa gcaggaaggg atatttggtt
     6721 ttatcggcaa taattttggt ggtaaaacgc gccagcacgt ccgggcggtc gagataatac
     6781 gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa gcgcatcgcc tgactgataa
     6841 agcccttcca gcgcggtatt cgccgccggg cttatttcat taacgatctt aagcgtaaaa
     6901 tgctccggca aattactgat gaccagtgcg ccctcttctt ctttccaggc ggtccacggc
     6961 tcatcattaa tatgaacaga aaccagtttg aggtcttcgc cgttgagacg aagtggagca
     7021 tctgatgcac catgacggac agcctggctg accgcggtaa cgaccgtctt ttgcgcgtcg
     7081 aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg gcgcacgata atcgtgacgg
     7141 tatttggctt gtggctgttg agtcataaat aacctttagc atcttttata gagtctggtg
     7201 ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat cttcatcttt tcaggtacaa
     7261 acgcctttat tgctacattt ttataacata caccgcgtaa tgccatcgac cagaaaggtg
     7321 gcatatggtg tgatcggggt tcaataaatt gcgaaacaag gtatactcca gcagttcctg
     7381 aagatgttta ttgtactaaa cgctcctgta cgaggacgct actgcgcacc tatgacacaa
     7441 ttcgcttctc ctgttctgca ctcgttgctg gatacagatg cttataagtt gcatatgcag
     7501 caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg cggagtttcg ttgccgaggt
     7561 gacgatctgc tgggtattta tgccgatgct attcgtgaac agattcaggc gatgcagcac
     7621 ctgcgcctgc aggatgatga atatcagtgg ctctctgccc tgcctttctt taaggccgac
     7681 tatcttaact ggttacgcga gttccgcttt aacccggaac aagtcaccgt gtccaacgat
     7741 aatggcaagc tggatattcg cttaagcggt ccgtggcgtg aagtcatcct ctgggaagtt
     7801 cctttgctgg cggttatcag tgaaatggta catcgctatc gctcaccgca ggccgacgtt
     7861 gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact tctcggcgtt aaccgccggt
     7921 cttgatatgt cgcgcttcca tctgatggat tttggcaccc gtcgccgttt ttctcgcgaa
     7981 gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct ggttcgtggg caccagcaac
     8041 tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa cacaggcaca cgaatggttc
     8101 caggcacatc agcaaatcag cccggatcta gccaacagcc agcgagctgc acttgctgcc
     8161 tggctggaag agtatcccga ccaacttggc attgcattaa ccgactgcat cactatggat
     8221 gctttcctgc gtgatttcgg tgtcgagttc gctagtcggt atcagggcct gcgtcatgac
     8281 tctggcgacc cggttgaatg gggtgaaaaa gccattgcac attatgaaaa gctgggaatt
     8341 gatccacaga gtaaaacgct ggttttctct gacaatctgg atttacgcaa agcggttgag
     8401 ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg gtattgggac tcgcctgacc
     8461 tgcgatatcc cccaggtaaa acccctgaat attgtgatta agttggtaga gtgtaacggt
     8521 aaaccggtgg cgaaactttc tgacagccct ggcaaaacta tctgccatga taaagcgttt
     8581 gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta aaaaagccag ttaatatcat
     8641 cagggagcta atcggctccc tttttttacc tttaattccg aaatctttcg ctgcatttgc
     8701 gaattctgct tgtctgattg cagatgccag gtaacatagg tatccccccc atttacgggt
     8761 ggacaagtgt ttattttttc cgactattaa cagagagaat attatgagcg ttgtgcctgt
     8821 agccgacgta ctccagggcc gcgtagccgt tgacagcgaa gtcaccgtac gcggatgggt
     8881 acgtacccgc cgagattcaa aagctggcat ctccttcctc gccgtttatg acggttcctg
     8941 ctttgatcct gtacaggctg tcatcaataa ttctctgccc aattacaatg aagacgtcct
     9001 gcgtctgacc accggctgct cggtcattgt gacgggtaaa gtcgtggcat cgccgggcca
     9061 ggggcaacaa tttgaaattc aggccagcaa ggttgaagtt gctggttggg ttgaagatcc
     9121 agacacttac ccgatggcgg caaaacgcca cagcattgag tatctgcgtg aagtcgctca
     9181 cctgcgtccg cgcacaaacc tgattggtgc cgtcgcgcgc gttcgccata cgctggcgca
     9241 ggcgctgcat cgcttcttta acgagcaggg attcttctgg gtttcaacgc cactgattac
     9301 cgcatctgat accgaaggtg caggcgaaat gttccgcgtt tctacgctgg atctggaaaa
     9361 cctgccgcgt aacgatcagg gcaaagtgga tttcgacaaa gacttctttg gtaaagagtc
     9421 tttcctgacc gtatctggcc agttgaacgg cgaaacctac gcttgcgcat tatccaaaat
     9481 ttataccttc ggcccgactt tccgtgctga aaactccaac accagccgtc acctggcgga
     9541 attctggatg ctggagccgg aagtggcgtt tgctaacctg aacgatatcg cgggtctggc
     9601 tgaagccatg ctgaaatatg tcttcaaagc ggttctcgaa gaacgcgcag acgacatgaa
     9661 attcttcgct gaacgcgtag ataaagatgc cgtttcacgt ctggaacgct tcattgaggc
     9721 cgatttcgcg caggtggatt acaccgacgc agtgaccatt ctcgaaaact gcggcaggaa
     9781 gtttgaaaac ccggtttact ggggcgtcga tctctcttct gagcatgagc gttatctggc
     9841 ggaagaacac tttaaagcac cggtagtggt taaaaactat ccgaaagata ttaaagcgtt
     9901 ctatatgcgc ctcaacgaag acggtaaaac cgttgcggct atggacgttc tggctccggg
     9961 catcggtgag atcattggtg gctcccagcg tgaagaacgt ctggacgttc tggacgagcg
    10021 tatgctggaa atgggcctga ataaagaaga ttactggtgg tatcgcgatc tgcgtcgcta
    10081 cggtactgtt ccgcattcag gtttcggtct tggttttgaa cgcctgattg cttacgtaac
    10141 tggcgtgcaa aacgtgcgtg atgtgattcc gttcccacgt actccgcgca acgccagctt
    10201 ctgattttct tcctcaaaga gccagcgcct gctggctctt ttttatcctt tctgtcaagt
    10261 tatctgtttg ttaagtcaag caatctattt gcaaccccgc cataacgatc atcctgttac
    10321 ggaatattac attgcaacat ttacgcacaa aaaataaccc gcattcttat tgcggattag
    10381 ttttttctta gctgatagca caattttcat actatttttt ggcattctgg atgtctgaaa
    10441 gag
//
LOCUS       DAZPLM010000144        10388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPLM010000144 DAZPLM010000000
VERSION     DAZPLM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.144"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            163..1557
                     /gene="galP"
                     /locus_tag="ACHVC7_003066"
     CDS             163..1557
                     /gene="galP"
                     /locus_tag="ACHVC7_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR2117050.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1634..2131
                     /locus_tag="ACHVC7_003067"
     CDS             1634..2131
                     /locus_tag="ACHVC7_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR2117051.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2226..2933
                     /gene="endA"
                     /locus_tag="ACHVC7_003068"
     CDS             2226..2933
                     /gene="endA"
                     /locus_tag="ACHVC7_003068"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR2117052.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3013..3744
                     /gene="rsmE"
                     /locus_tag="ACHVC7_003069"
     CDS             3013..3744
                     /gene="rsmE"
                     /locus_tag="ACHVC7_003069"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR2117053.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3757..4707
                     /gene="gshB"
                     /locus_tag="ACHVC7_003070"
     CDS             3757..4707
                     /gene="gshB"
                     /locus_tag="ACHVC7_003070"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR2117054.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4816..5379
                     /locus_tag="ACHVC7_003071"
     CDS             4816..5379
                     /locus_tag="ACHVC7_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR2117055.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5379..5795
                     /gene="ruvX"
                     /locus_tag="ACHVC7_003072"
     CDS             5379..5795
                     /gene="ruvX"
                     /locus_tag="ACHVC7_003072"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR2117056.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5970..6950)
                     /locus_tag="ACHVC7_003073"
     CDS             complement(5970..6950)
                     /locus_tag="ACHVC7_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR2117057.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            6968..7672
                     /gene="yggS"
                     /locus_tag="ACHVC7_003074"
     CDS             6968..7672
                     /gene="yggS"
                     /locus_tag="ACHVC7_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR2117058.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7690..8256
                     /gene="yggT"
                     /locus_tag="ACHVC7_003075"
     CDS             7690..8256
                     /gene="yggT"
                     /locus_tag="ACHVC7_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR2117059.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8253..8543
                     /gene="yggU"
                     /locus_tag="ACHVC7_003076"
     CDS             8253..8543
                     /gene="yggU"
                     /locus_tag="ACHVC7_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR2117060.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8551..9144
                     /locus_tag="ACHVC7_003077"
     CDS             8551..9144
                     /locus_tag="ACHVC7_003077"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR2117061.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9137..10273
                     /gene="hemW"
                     /locus_tag="ACHVC7_003078"
     CDS             9137..10273
                     /gene="hemW"
                     /locus_tag="ACHVC7_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR2117062.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDNALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2441 a         2728 c         2743 g         2476 t
ORIGIN      
        1 ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac taagataaat
       61 gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc
      121 tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa
      181 caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga
      241 ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa
      301 ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca
      361 gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg
      421 atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa
      481 gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca
      541 ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat
      601 cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac
      661 accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt
      721 ggtgtcttct tcctgccaga cagcccacgt tggtttgccg ccaaacgccg ttttgttgat
      781 gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat
      841 gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc
      901 aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg
      961 atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc
     1021 acagagcaaa tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc
     1081 gcaatcggcc ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg
     1141 atggctgctg gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg
     1201 gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc
     1261 ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc
     1321 atcacctgct ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg
     1381 accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta
     1441 ctgtttatcc tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat
     1501 attgaacgta acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct
     1561 cctcaggctt cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct
     1621 atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg
     1681 cggcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc
     1741 aaaactctct tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga
     1801 aatccgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt
     1861 accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg
     1921 caaagagtgg aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca
     1981 gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca
     2041 tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt
     2101 tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat
     2161 ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt
     2221 aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc
     2281 cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc
     2341 acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag
     2401 gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc
     2461 gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc
     2521 aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata
     2581 tgcataacct gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca
     2641 gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag
     2701 aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata
     2761 tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga
     2821 acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc
     2881 aaggcaatca taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac
     2941 tagcgggatt ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc
     3001 ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct
     3061 cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg
     3121 gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc
     3181 gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct
     3241 ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc
     3301 cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc
     3361 gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt
     3421 gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg
     3481 gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt
     3541 aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa
     3601 ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg
     3661 ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa
     3721 gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg
     3781 gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca
     3841 cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc
     3901 gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg
     3961 ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac
     4021 ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa
     4081 gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc
     4141 gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa
     4201 gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg
     4261 tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact
     4321 gagcatggca ctcgctactg catggcacaa aattacctgc cagccattaa agatggcgac
     4381 aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag
     4441 gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg
     4501 gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt
     4561 tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc
     4621 tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc
     4681 atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc
     4741 tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa
     4801 acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag
     4861 gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg
     4921 gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg
     4981 aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc
     5041 ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc
     5101 agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc
     5161 ggcaccgata aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa
     5221 ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat
     5281 attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg
     5341 gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct
     5401 tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc
     5461 gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt
     5521 tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca
     5581 ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg
     5641 gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt
     5701 ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta
     5761 ttattctcga aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag
     5821 tcgttaccgc catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg
     5881 attgtaggtc tgataagacg cgcaaacgtc tcatcagacg tttattgcct ttgtaggccc
     5941 gataagcttg cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga
     6001 aaactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac
     6061 tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca
     6121 aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt
     6181 aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc
     6241 agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg
     6301 gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa
     6361 atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc
     6421 aaaccaattt cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga
     6481 tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc
     6541 agcgtggtag atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc
     6601 ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa
     6661 ggtaacagcc gtaacgccag cgaaatgcca tgccgttgcg caaatgcgct gccacgcaat
     6721 cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc
     6781 cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc ctcaaacggc
     6841 gcagcttcca ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga
     6901 tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc
     6961 tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc
     7021 tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa
     7081 acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta
     7141 cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga
     7201 atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga
     7261 ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc
     7321 agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc
     7381 cgggattcaa ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt
     7441 acgtctgcgc gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga
     7501 agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga
     7561 cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat
     7621 ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa
     7681 aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg
     7741 gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca
     7801 cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca
     7861 atgggcccca ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc
     7921 atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt
     7981 ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc
     8041 tgggtaagcc agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg
     8101 ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc
     8161 ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga
     8221 aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg
     8281 gtctggtttt acggctctat attcagccga aagccagccg tgattctatt gtcggtttac
     8341 atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc
     8401 atctggtgaa gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa
     8461 aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag
     8521 aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat
     8581 gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc
     8641 caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg
     8701 attctgaaag cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct
     8761 ggtctggcgg tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt
     8821 gaagacgcga ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg
     8881 gacgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat
     8941 ccgactccgc tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc
     9001 actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc
     9061 gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg
     9121 ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg
     9181 gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc
     9241 gcacgacgat tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca
     9301 gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc
     9361 ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga
     9421 aattactatg gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg
     9481 tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg
     9541 acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct
     9601 ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga
     9661 ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca
     9721 actgaccatc gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgataa
     9781 cgcgctgtgg gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca
     9841 atatgaaact tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg
     9901 gcgatttggt gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga
     9961 tgggcgcatt ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta
    10021 tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg
    10081 cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga
    10141 agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc
    10201 ggattactgg cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt
    10261 cctcgctgag taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt
    10321 aattaacttc ggcaaaatat atttcttact gaataaacat cgatgatatt tattagatat
    10381 atctagtg
//
LOCUS       DAZPLM010000145        10291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPLM010000145 DAZPLM010000000
VERSION     DAZPLM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.145"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            49..1731
                     /locus_tag="ACHVC7_003079"
     CDS             49..1731
                     /locus_tag="ACHVC7_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR2117063.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1783..2940
                     /locus_tag="ACHVC7_003080"
     CDS             1783..2940
                     /locus_tag="ACHVC7_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR2117064.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2906..3028)
                     /locus_tag="ACHVC7_003081"
     CDS             complement(2906..3028)
                     /locus_tag="ACHVC7_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117065.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            3237..4922
                     /locus_tag="ACHVC7_003082"
     CDS             3237..4922
                     /locus_tag="ACHVC7_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR2117066.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            4960..7332
                     /locus_tag="ACHVC7_003083"
     CDS             4960..7332
                     /locus_tag="ACHVC7_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR2117067.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7389..10172
                     /locus_tag="ACHVC7_003084"
     CDS             7389..10172
                     /locus_tag="ACHVC7_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR2117068.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3039 a         2267 c         2244 g         2741 t
ORIGIN      
        1 cttcgtaaaa ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca
       61 ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca
      121 tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc
      181 tcagatttta cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc
      241 atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca
      301 atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct
      361 gcacaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac
      421 ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct
      481 cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa
      541 actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg
      601 cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat
      661 gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac
      721 tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat
      781 cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc
      841 gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct
      901 ccgcacctac caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt
      961 agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc
     1021 attctcgaac aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc
     1081 gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag
     1141 agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt
     1201 caacccggta attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat
     1261 gcagccgata tcagcattcc aaaagacctt aagctggatg gtgtttcctt gctgccctgg
     1321 ttgcaagata agaaacaagg cgcgccacat aaaaatctga cctggataac ctcttattct
     1381 cactggtttg atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat
     1441 cagtcagacg attacccgca taaccccaac actgaggact taagccaatt ctcttatacg
     1501 gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac
     1561 aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa
     1621 gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta
     1681 aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt
     1741 catgcggcgg atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag
     1801 ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg
     1861 cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat
     1921 atcgaagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct
     1981 ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt
     2041 atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc
     2101 aaagaacatg aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt
     2161 tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt
     2221 ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat
     2281 taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc
     2341 gaattgctag aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc
     2401 cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt
     2461 atgcaatggg ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc
     2521 agccgttttt tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat
     2581 ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac
     2641 aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg
     2701 caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc
     2761 ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa
     2821 ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct
     2881 aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa
     2941 taatgctctc ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga
     3001 cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac
     3061 gtggtatgcg tcagggcatt agcaataccc ttgattatca atagcataac cataaccata
     3121 accactgaaa attaaaaatt tctatatatt tcatattgtt aaatgatact taaataaatt
     3181 tcatcagtaa atcattgcca tagataatca ttggatggca caatcaattt tcgctaatga
     3241 taaccattcc cattacgttt tatatgttga tagcaaagta tctctgtctg ctgaagccct
     3301 tttggctgcg taagaataat aaaacttcag tgttgttaat cattattatc ctcgcaatga
     3361 tccttggcgt ggttaaaatt caggtctggt taaacgactg gaataatgat tttttcaatg
     3421 cgctaagcca gaaagaaacc gataagcttt ggcagcttgt tctttggttc cctgccctgc
     3481 tggggatctt tgtattaata tccgtaaaca aaacatggtt aattaaacta ctgactattc
     3541 gctggcgcga atggttaaca gattactacc ttaatcgctg gtttgcagat aaaaactact
     3601 acttcacgca gatctacggc gagcataaaa atacagataa ccctgaccaa cgtatcgctg
     3661 aagacattct cttactgatc agcaaaacgc tcagtctttc atttggcttc atccagtcac
     3721 ttagcatgct gatcaccttt accgttattc tttggcaaag cgcgggtacg ctctcattca
     3781 ctgttggtgg aacagaatgg aatatccagg ggtatatggt ctataccgtc gtgctcattg
     3841 tgatcggtgg aactttattt acccataagg tgggtaaacg tattcgtccg cttaatgtgg
     3901 aaaaacaacg tagcgaagcg acatttcgaa ctaatcttgt gcagcataat aagcagacag
     3961 aactgattgc gctaagcaat gctgagtctc tacaacgcca ggagttgcgc gacaattttc
     4021 ataccattaa agagaactgg catcgtttaa tgaatcgtca gcggtggctt gattactggc
     4081 aaaatattta ttcgcgctcg cttagcgttc ttccctactt tctgttattg ccgcagttta
     4141 ttagcgggca gatcaatctg ggcggactga tgaaatcgcg ccaggcattt atgctagtat
     4201 cgaacaattt aagctggttt atttataaat atgacgaact tgctgaactg gctgcggtta
     4261 tcgatcgctt gtatgagttc catcaactca ctgaacagcg ccctacgaat aagcctaaaa
     4321 attgccaaca tgcggtacaa gtggctaatg cgagtattcg tacgcctgat aataagatca
     4381 tattagagaa cttgaacttt catgtttcgc caggcaaatg gctattactg aaaggctact
     4441 ctggtgcggg aaaaaccaca ctgcttaaaa cattatccca ctgctggccg tggtttaaag
     4501 gtgatatttc ttctcctgct gacagttggt atgtgtcaca aacaccgtta atcaaaagcg
     4561 gcttactgaa agagattatt tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga
     4621 gcgaagtact gcatcaggtt ggtctgggga aattggctgc gcgtattcat gaccacgatc
     4681 gctggggaga tattctttcc agcggcgaaa aacaacgtat cgccctggca cgattaattt
     4741 tacgacgtcc gaaatggata tttcttgacg aaactacctc tcatcttgag gaacaagagg
     4801 ctatacgctt actgcgttta gtgcgtgaaa aactacccac aagcggcgtc attatggtta
     4861 cacatcaacc cggtgtctgg aacctggctg atgatatttg tgacattagc acggttttat
     4921 aacaacaata aaagccctgc tgtacggaga tataaataaa tgaagcgagt tcttattcct
     4981 ggcgtcattt tatgtggcgc tgatgtggcg caggccgtcg atgacaaaaa catgtacatg
     5041 catttttttg aagagatgac ggtctatgct cctgtccctg tacccgtaaa cggcaacacg
     5101 cattacacca gtgaaagcat cgagcgttta ccgaccggga atggcaatat cagcgatctg
     5161 ctgagaacca accctgcggt acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga
     5221 gatattcgcc ctgagaaaat ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg
     5281 attgacggta ttagtgcaac taataacctg aacccagcga atgagtccga tgccagtagt
     5341 gcaaccaata ttagcgggat gtcacagggg tattatcttg atgtcagctt actggacaat
     5401 gtgacgcttt atgacagttt tgtgccggtt gaatttggtc gcttcaatgg cggggtaatt
     5461 gatgcaaaga tcaaacgctt caacgctgat gatagcaagg tgaaattggg ttatcgcact
     5521 acgcgttcgg actggttaac atcgcatatc gatgagaata acaagagcgc atttaatcaa
     5581 ggctcttcag gaagtactta ttactcccca gattttaaaa agaactttta taccttgtcg
     5641 tttaatcagg aactcgctga taacttcggc gttaccgccg gtttatcgcg ccgccagtct
     5701 gatatcaccc gcgcggatta tgtttcgaat gacggcattg tcaccggtcg ggcacagtat
     5761 aaaaacgtta tcgatactgc attgagcaaa tttacctggt ttgccagcga ccgctttacc
     5821 cacgatttaa ccttaaaata taccggctcc agccgtgatt ataataccag caccttcccg
     5881 cagtctgatc gcgaaatggg taataaatcc tatggtctgg catgggatat ggatacgcaa
     5941 ctcgcatggg ccaaactacg taccaccgtt ggttgggatc atattagtga ttatacccgt
     6001 cacgatcatg acatctggta caccgaactt tcatgtacat atggtgatat tacagggcgt
     6061 tgtacctgtg gcggattagg acacatttcc caggctgtag ataattacac cttcaaaaca
     6121 cgcctggact ggcaaaaatt cgccgtgggt gatgtttcgc atcaacccta cttcggcgcg
     6181 gaatacatct attccgatgc atggactgaa cgccataacc agtctgaatc ctatgtgatt
     6241 aatgctgcag gaaagaaaac taaccatacc atttaccata aaggtaaagg cagcctggga
     6301 attgacaact acacgctgta tatggcggag cacattagct ggcggaatgt gtcgttaatg
     6361 cccggtgtgc gttatgacta tgacaactat ctgtcaaacc acaatatctc cccgcgcttt
     6421 atgacggaat gggatatttt tgctgatcaa acctcaatga ttaccgccgg ttataaccgt
     6481 tactatggcg ggaatattct tgatatggga ttacgtgata tccgcaatag ctggacggaa
     6541 tcggtatcag gtaataaaac tctgacgcgt tatcaggatt tgaaaacgcc ttataacgat
     6601 gaactggcaa tgggattgca gcagaaaatc ggtaagaacg ttattgcgcg tgcaaactat
     6661 gtttaccgtg aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag cgcgactaaa
     6721 accaccatta ctgaatataa caacgatggc aaaaccaaaa cgcattcgtt cagcctcagt
     6781 tttgaactgg ccgaacccct gcatatccgc caagtagata ttaacccaca aattgtcttt
     6841 agctatatca agagcaaggg caacttgtcg ttaaacaatg gttatgagga gagcaatacc
     6901 ggtgataacc aggtggttta taacggtaat ctggtctctt acgatagtgt tccagtggca
     6961 gattttaata acccattaaa gatctcctta aacatggatt tcacgcatca accgagcggg
     7021 ttagtgtggg cgaatacgct ggcctggcaa gaagcgcgta aagctcgcat tatccttggt
     7081 aagacaaatg cgcaatacat cagcgaatat tcagattaca agcagtatgt tgacgaaaaa
     7141 ctggatagca gcctgacctg ggacacccgc ttgtcctgga cgccacaatt tctgaaacaa
     7201 caaaacctga cgatcagtgc cgatattctc aatgtactgg atagcaaaac cgctgttgat
     7261 acaacgaaca ccggtgtggc gacctacgcc agtggccgta ctttctggct tgatgtcagc
     7321 atgaaatttt aatagttcgg attttactta accgggtaaa ttcgcccggt tttcgcatgg
     7381 agataattat gagaaacctc tgtttcttac tgacgttagt ggcaactctg ttgctccccg
     7441 ggcggctgat tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg caactggaca
     7501 atggcttgcg atatatgatt tatccgcatg ctcaaccaaa ggatcaggta aatttatggc
     7561 tgcaaattca taccggttca ttgcaggaag aagacaatga gcgcggcgtg gctcattttg
     7621 tagaacatat gatgtttaac ggcacaaaaa catggccggg taataaagtc atcgaaacat
     7681 ttgagtcaat gggcctgcgt ttcggtcgcg atgttaatgc ctataccagc tatgacgaaa
     7741 cgctgtatca ggtgagtctg ccgactacgc agaaacaaaa tctgcaacaa gtgatggcaa
     7801 tcttcagtga atggagtaat gccgcaacct ttgaaatact cgaagtagac gctgaacgcg
     7861 gcgtaattac tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc
     7921 gccgcccttt cctgctggca aatacccgta atttagaccg tgaacctatc ggcctgatgg
     7981 atactgtcgc cacggtcaca ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac
     8041 caaataatat gacctttatc gtggtcggcg atatcgacag taaagaagcg ctggcgctga
     8101 taaaggataa tttaagtaag cttccggcta acaaagcagc tgaaaatcgc gtctggccga
     8161 caaaagccga aaaccacctg cgctttaata tcattaatga taaagaaaac cgagtgaacg
     8221 gcatcgcact ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa agctttgtcg
     8281 aacaagctga atggagcatg ttagttcagc tgttcaatca acgtctgcag gaacgcatac
     8341 agtcgggcga gttgaagact atttctggcg gcactgcgcg cagcgttaaa attgcacccg
     8401 attatcagtc gctgtttttc cgtgtaaatg cacgagacga taatatgcag gatgctgcaa
     8461 atgcattaat ggcagagttg gcaaccattg atcagcatgg tttttctgct gaagaactcg
     8521 atgatgtcaa atctacccgc ctcacctggc tgaaaaatgc ggttgatcag caagctgaac
     8581 gtgatttacg tatgctgacc agtcgcctgg catccagctc attaaataat acgccgttct
     8641 tgtcgccgga agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa
     8701 gtctggcgga aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg
     8761 taaacaatga gcttgccgcc aaaaaagcat tgtctcttgc ggctatcctg gcgctggaaa
     8821 aagagtacgc caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa
     8881 cagtagacgc tgacccacag gcggaaatta gcagcaaaga aacgctggct gagaatctga
     8941 catcattaac actttccaat ggtgccaggg ttattctggc aaaatccgcg ggtgaagagc
     9001 aaaagctaca aattactgcc gtatctaata aaggcgattt aagtttccct gcgcagcaaa
     9061 aatcacttat cgcgctggca aataaagcag taagcggaag cggcgttggt gagctctcct
     9121 cttccagcct gaaacgctgg agtgcggaaa attcggtaac catgagcagt aaagtcagtg
     9181 gcatgaatac gttgctctcc gttagcgcgc ggactaataa ccctgaacct ggttttcagt
     9241 tgattaacca gcgaatcacc cacagcacga ttaacgacaa tatttgggca tcgctacaaa
     9301 atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc
     9361 agatgtatga gacgcgctat gctgatgacc gcacgaaatt actgcaagaa aatcagattg
     9421 tacagtttac tgccgcagat gcgctggctg ccgatcgcca gttgttttca tctccagcgg
     9481 atatcacgtt tgtcattgtc ggtaatgtct cagaagacaa actcgtggcg ttaattacgc
     9541 gttacttagg atcaatcaaa cactctgatt cgccattagc cgcaggtaaa ccattaactc
     9601 gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa tgaacctgtg gcacaggttt
     9661 cacagtggaa gcgttatgat tcccggacac ctgttaatct ggcgacgcgt atggcgctcg
     9721 atgcttttaa cgtcgcactg gcaaaagatc tacgcgttaa tattcgtgaa caggcatctg
     9781 gagcatacag cgtttcttct cgcctctcgg ttgatcctca ggccaaagat atcagtcatt
     9841 tgctggcttt tacttgtcaa ccagaacgac atgatgaact gttaacgtta gcgaatgaag
     9901 tgatggttaa gcgtctggct aaagggatca gtgagcaaga actgaatgaa taccagcaaa
     9961 acgttcagcg cagcctcgat atccaacagc gtagcgttca acaattagcg aacactattg
    10021 taaatagtct tattcaatat gacgatcctg cagcatggac tgagcaggag caattgttga
    10081 aacaaatgac ggtagagaat gttaacactg ccgttaaaca atatctttct catccggtaa
    10141 atacttatac tggagtatta ttgccaaaat aataacagcc ccgtcaacac atcgttggcg
    10201 gggattttag caatattcgc catttttatg taataatttt ataaatgcgt tcaaaataat
    10261 aaccaggtac taatagtgat attttaaggt c
//
LOCUS       DAZPLM010000146        10097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPLM010000146 DAZPLM010000000
VERSION     DAZPLM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.146"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHVC7_003085"
     CDS             <1..807
                     /locus_tag="ACHVC7_003085"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR2117069.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="ACHVC7_003086"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="ACHVC7_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR2117070.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="ACHVC7_003087"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="ACHVC7_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR2117071.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHVC7_003088"
     CDS             complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHVC7_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR2117072.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHVC7_003089"
     CDS             complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHVC7_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR2117073.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="ACHVC7_003090"
     CDS             7815..8093
                     /locus_tag="ACHVC7_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117074.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHVC7_003091"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHVC7_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR2117075.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHVC7_003092"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHVC7_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR2117076.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2689 a         2370 c         2353 g         2685 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttc tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtat
//
LOCUS       DAZPLM010000147        10029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPLM010000147 DAZPLM010000000
VERSION     DAZPLM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.147"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(56..1113)
                     /locus_tag="ACHVC7_003093"
                     /pseudo
     CDS             complement(56..1113)
                     /locus_tag="ACHVC7_003093"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(1204..1899)
                     /gene="rsuA"
                     /locus_tag="ACHVC7_003094"
     CDS             complement(1204..1899)
                     /gene="rsuA"
                     /locus_tag="ACHVC7_003094"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR2117077.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2049..3809
                     /locus_tag="ACHVC7_003095"
     CDS             2049..3809
                     /locus_tag="ACHVC7_003095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR2117078.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            3934..4218
                     /gene="rplY"
                     /locus_tag="ACHVC7_003096"
     CDS             3934..4218
                     /gene="rplY"
                     /locus_tag="ACHVC7_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR2117079.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4357..5364)
                     /gene="yejK"
                     /locus_tag="ACHVC7_003097"
     CDS             complement(4357..5364)
                     /gene="yejK"
                     /locus_tag="ACHVC7_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR2117080.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5546..5773
                     /locus_tag="ACHVC7_003098"
     CDS             5546..5773
                     /locus_tag="ACHVC7_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR2117081.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5793..7553
                     /gene="yejM"
                     /locus_tag="ACHVC7_003099"
     CDS             5793..7553
                     /gene="yejM"
                     /locus_tag="ACHVC7_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR2117082.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7628..7704
                     /locus_tag="ACHVC7_003100"
     tRNA            7628..7704
                     /locus_tag="ACHVC7_003100"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7662..7664,aa:Pro,seq:ggg)
     gene            complement(7807..10029)
                     /gene="yejO"
                     /locus_tag="ACHVC7_003101"
                     /pseudo
     CDS             complement(7807..10029)
                     /gene="yejO"
                     /locus_tag="ACHVC7_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         2493 a         2711 c         2414 g         2411 t
ORIGIN      
        1 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
       61 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
      121 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
      181 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
      241 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
      301 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
      361 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
      421 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
      481 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
      541 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
      601 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
      661 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
      721 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
      781 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
      841 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
      901 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
      961 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
     1021 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
     1081 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
     1141 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
     1201 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
     1261 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
     1321 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
     1381 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
     1441 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
     1501 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
     1561 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
     1621 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
     1681 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
     1741 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
     1801 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
     1861 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
     1921 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
     1981 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
     2041 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
     2101 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
     2161 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
     2221 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
     2281 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
     2341 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
     2401 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
     2461 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
     2521 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
     2581 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
     2641 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
     2701 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
     2761 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
     2821 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
     2881 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
     2941 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
     3001 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
     3061 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
     3121 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
     3181 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
     3241 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
     3301 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
     3361 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
     3421 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
     3481 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
     3541 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
     3601 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
     3661 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
     3721 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
     3781 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
     3841 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
     3901 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
     3961 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
     4021 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
     4081 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
     4141 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
     4201 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt
     4261 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
     4321 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
     4381 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
     4441 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
     4501 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
     4561 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
     4621 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
     4681 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
     4741 gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgtgttca atccttcgct
     4801 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
     4861 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
     4921 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
     4981 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
     5041 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
     5101 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
     5161 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
     5221 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
     5281 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
     5341 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
     5401 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
     5461 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
     5521 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
     5581 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
     5641 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
     5701 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
     5761 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
     5821 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
     5881 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
     5941 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
     6001 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
     6061 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
     6121 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
     6181 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
     6241 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
     6301 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
     6361 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
     6421 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
     6481 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
     6541 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
     6601 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
     6661 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
     6721 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
     6781 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
     6841 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
     6901 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
     6961 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
     7021 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
     7081 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
     7141 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
     7201 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
     7261 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
     7321 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
     7381 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
     7441 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
     7501 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
     7561 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
     7621 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
     7681 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
     7741 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
     7801 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
     7861 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
     7921 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
     7981 aactttcaca tggttagaat cggcctattc ctgacgcacg gcggctttca gccagggttc
     8041 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
     8101 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
     8161 atctgtggta aagccggtaa aggcgagata ggggctaaca ctccacaatc cgtcaaccca
     8221 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
     8281 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
     8341 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
     8401 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
     8461 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
     8521 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
     8581 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
     8641 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
     8701 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
     8761 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
     8821 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
     8881 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
     8941 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
     9001 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
     9061 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
     9121 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
     9181 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
     9241 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
     9301 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
     9361 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
     9421 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
     9481 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
     9541 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
     9601 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
     9661 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
     9721 ccgccactat ccacagtggt attataagcg aagtcatttt cttcaactcg caaacttccg
     9781 ccgttttcca acagcatatt gttagcaata ccattcttga tttcgaactg accgagacgg
     9841 tttgttccga atacctccat gacccgcgtg gttgttttaa tagcaccgcc tgctttctga
     9901 tctaccgcaa atgctaatcc cccgtcttta acctccagta atcctccctt ttcgatagcg
     9961 gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat cgtgggatca
    10021 ttcgccgcg
//
LOCUS       DAZPLM010000148         9955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPLM010000148 DAZPLM010000000
VERSION     DAZPLM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.148"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..859
                     /locus_tag="ACHVC7_003102"
     CDS             <1..859
                     /locus_tag="ACHVC7_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2117083.1"
                     /translation="AVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRS
                     VLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLS
                     LFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMI
                     PHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAM
                     TIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNF
                     RIHLLNALR"
     gene            994..2214
                     /gene="mlc"
                     /locus_tag="ACHVC7_003103"
                     /gene_synonym="dgsA"
     CDS             994..2214
                     /gene="mlc"
                     /locus_tag="ACHVC7_003103"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR2117084.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2339..3034
                     /gene="bioD"
                     /locus_tag="ACHVC7_003104"
     CDS             2339..3034
                     /gene="bioD"
                     /locus_tag="ACHVC7_003104"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR2117085.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(2987..4279)
                     /gene="clcB"
                     /locus_tag="ACHVC7_003105"
                     /pseudo
     CDS             complement(2987..4279)
                     /gene="clcB"
                     /locus_tag="ACHVC7_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(4438..5052)
                     /gene="dmsD"
                     /locus_tag="ACHVC7_003106"
     CDS             complement(4438..5052)
                     /gene="dmsD"
                     /locus_tag="ACHVC7_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR2117086.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5095..5949)
                     /gene="ynfH"
                     /locus_tag="ACHVC7_003107"
     CDS             complement(5095..5949)
                     /gene="ynfH"
                     /locus_tag="ACHVC7_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR2117087.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5951..6568)
                     /gene="ynfG"
                     /locus_tag="ACHVC7_003108"
     CDS             complement(5951..6568)
                     /gene="ynfG"
                     /locus_tag="ACHVC7_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR2117088.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(6579..9002)
                     /gene="ynfF"
                     /locus_tag="ACHVC7_003109"
     CDS             complement(6579..9002)
                     /gene="ynfF"
                     /locus_tag="ACHVC7_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR2117089.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSYTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9063..>9955)
                     /locus_tag="ACHVC7_003110"
     CDS             complement(9063..>9955)
                     /locus_tag="ACHVC7_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR2117090.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2442 a         2546 c         2587 g         2380 t
ORIGIN      
        1 tgctgttgcg gaagagctgc atttcgggcg cgccgctgcc cgcctgaata tttcgcaacc
       61 accgctaagt cagcagattc aggcactgga gcaacaaatt ggtgcccgac tgctggcacg
      121 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
      181 aatcctgtct atggtagatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc
      241 gggggagttg cgcattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
      301 gttatcgctg tttcgccgtg attatcccga tgtccattta caaacccgcg aaatgaacac
      361 tcgtgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
      421 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
      481 cccgcacgat catcccctgg caaataaccc gaatgtaacg ctggctgaac tggcgaaaga
      541 acccttcgtc ttttttgatc cgcacgtcgg gacagggctg tatgacgata ttctcgggct
      601 gatgcgacgt taccatttga cgcccgtcat cactcaggag gtgggcgagg caatgaccat
      661 catcggtctg gtttccgccg gtctgggtgt ttcaattttg cctgcttcat ttaaacgtgt
      721 tcagctcaac gaaatgcgct gggtgccgat tgctgaagag gatgcggttt ctgaaatgtg
      781 gttggtctgg ccgaaacatc atgaacaaag tccagcttcg cgtaactttc gtattcatct
      841 gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc ccgaaaaatg tgctgttaat
      901 cacatgccta agtaaaaatt tgacgacacg tattgaagtg cttcaccata gcctacagat
      961 tatttcggag cgcgaaaata tagggagtat gcggtggttg ctgaaaacca gcttgggcac
     1021 attgatcaaa taaagcagac caacgcgggc gcggtttatc gcctgattga tcagcttggt
     1081 ccagtctcgc gtatcgatct ttcccgtctg gcgcagctgg ctcctgccag tatcactaaa
     1141 attgtccgtg agatgctcga agcacacctg gtgcaagagc tggaaatcaa agaagcgggg
     1201 aaccgtggcc gtccggcggt ggggctggtg gttgaaactg aagcctggca ctatctttct
     1261 ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg atctgagcag caaactggtg
     1321 gtggaagagt cgcaggaact ggcgttaaaa gatgacttgc cattgctgga tcgtatcatt
     1381 tcccatatcg atcagttttt tatccgccac cagaaaaaac ttgagcgtct aacttcgatt
     1441 gccataaccc tgccgggaat tattgatacg gaaaatggta ttgtacatcg catgccgttc
     1501 tacgaggatg taaaagagat gccgctcggc gaggcgctgg agcagcatac cggcgtaccg
     1561 gtttatattc agcatgatat cagcgcatgg acgatggcag aggccttgtt tggtgcctca
     1621 cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca acgtgggggc gggcgtcatt
     1681 accgatggtc atctgctaca cgcaggcagc agtagtctcg tggaaatagg ccacacacag
     1741 gtcgacccat atggtaaacg ctgttattgc gggaaccacg gctgcctcga aaccatcgcc
     1801 agtgtggaca gtattcttga gctggcacag ctgcgtctca atcaatccat gagctcgatg
     1861 ttacatgggc aaccgttaac cgtggactca ttgtgtcagg cggcattgcg cggcgatcta
     1921 ctggcaaaag acatcattac cggggtgggc gcgcatgtcg ggcgcattct tgccatcatg
     1981 gtgaatttat ttaacccaca aaaaatactg attggctcac cgttaagtaa agcggcagat
     2041 atcctcttcc ctgtcatctc ggacagcata cgtcagcagg cccttcctgc gtatagtcag
     2101 cacattagcg ttgagagtac tcagttttct aaccagggca cgatggcagg cgctgcgctg
     2161 gtaaaagacg cgatgtataa cggttctttg ttgattcgtc tgttgcaggg ttaacatttt
     2221 ttaactgttc taccaaaatt tgcgctatct caatttgggc caggaaagca taacttagac
     2281 tttcaaggtt aattattttc ctagtttata tttgtgaagc ataacggtgg agttagtgat
     2341 gctggagcgt ttctttatta ccggtacaga cacttctgta gggaaaacgg tggtttcccg
     2401 cgcattgcta caagcgttag cctcccaggg aaaaacagtt gcgggatata aacccgtagc
     2461 gaaggggagc aaagagacac ccgaagggct gcgtaataaa gatgccctgg tgttgcagag
     2521 tgtttcaacc atcgaactgc cttatgaagc agttaatcct atcgcgttaa gcgaagaaga
     2581 aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc atttcaaacg gcctggcaaa
     2641 cctgaccgat aaagtcgatc atgtcgtggt agaagggact ggcggctggc gcagtctgat
     2701 gaatgatttg cgtccactct ctgaatgggt ggtgcaggaa cagttgccgg tgttgatggt
     2761 tgtcggtatt caggaaggtt gcattaacca tgcactgcta acagctcagg cgattgccaa
     2821 cgacgggctg ccgctcattg gctgggtggc taaccgaatc aacccaggac tggcgcatta
     2881 tgcggaagtc attgatgtgc tgggtaaaaa acttccggca ccgctcattg gtgaactgcc
     2941 ttatctgccg cgcgccgaac agcgagaact ggggcaatac atccgcttag ctatgctgcg
     3001 cagtgtgctg gcggtagata gagtcacggt gtaacgtccg cgaaattacc gacgcaatga
     3061 cgcaggcaat caataaaccg gggagtagct gatactcccc ggtcatttca catatcatca
     3121 acgtcgacat aatcggcgcg tgcgtggttg ccgccaacag tgtcgccatc ccggtcaatc
     3181 cgagtaaaag cgtaatttct tcgccatcag ggaaccataa tcccaggcta cgaccataca
     3241 acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa gacaccaccg ggtgcgccgg
     3301 aaccgctact cgccagcacg gcacacagtt tacaaaggaa gatcccggca atgatcatta
     3361 acagtggtgg ggcggttaaa aaggattgta cggtgctata gccgttgccc cacactgcag
     3421 gtgtgaacag ggaaagcaga cccacgatca acccgcctag tgccagttgc tagggcggcg
     3481 caagtttgag actcacaaat ccacgatgac aggcgttcat taacgttaac aacagtggtc
     3541 cacacagacc tgccagcaca cctgtactga taatcagcgc atagtcacta gcctgaaccg
     3601 ttactgagag ttgtacgctg tagagtaacg cgtcgctatg attaataaga ttgctaacca
     3661 gcaacgccac gacggcggaa ataatcaccg ggccgagaga ggccaacatc atagtgccaa
     3721 acagcacttc ggctataaat aaactgccag caagcggggc gcgataggcc gcagccattc
     3781 ccgccgcggc cccacaggcg atccataatt tccattcctg gcgtggcgta aaacgttggg
     3841 caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc accttcgcga ccaattgcac
     3901 tgccgctggt tactaccagc agcgaggcaa gcgatttaac caggcttgct gcgtaatcga
     3961 actgtccatc ggtttgcaac gcttccatgt aatcggtcgg cgcatgaggg cgttgttggg
     4021 taaatttctg ccagcccatc agcaacaaac ccgccgccag tccgccgagc gccggagtta
     4081 gcagccgtcg ccagggggaa aggtttgtcg ctgcattgac cagactgccg gagtcattat
     4141 tgaggaacaa ccactccagt agcagcatcg catgacgaaa cccggcaacg gcaaaggccg
     4201 cgagaatacc gacgactgtt gcgataagca gacggcggaa catagtgcgc agatccggat
     4261 agatgtgtag atgatgcatg agcagaaatg ggcaatgaaa cacgggcgac tattttgcgg
     4321 caaaagaggc caaccgcaaa agcagtgcgt gctttaagag ataaaaaaac gtgacattgt
     4381 aacttattat tgccgaatcg cgcgtaatcg tcaccatccg gcaatattac ggtgatccta
     4441 tcgaaagagt ggtttaaccg cgacaggaat caacagttgt gattgccatt gcgccagcgt
     4501 taagcgagcc agttcaccca gtgcacggta gaaagggtgt tctgcttttt cgataaaaac
     4561 atcaagaaaa cgtgttgacc acggaaaaag gtgccatgcc agcaattctt cgcattctgt
     4621 ctggcgacca ttctccgcca accacgcagc catcaacagc agtgaaccaa aatgatcttc
     4681 cggttcgttt tgcttcattt caaactgaat gcctttctcg cgcatccact gacgaagtgc
     4741 caatgttgaa tcgccaaaca gcacagattc gcgatccagc cagaccgatc cccacggcgg
     4801 agacggcagt gcccacgggc cgacaaacaa acgctgccag gcctgggcgt gagtctcttc
     4861 acactgtgtc tgaaaagccg tcaccagcgg cgctaatgac gcttctggta gaggccactg
     4921 agtttcccag ccgtcactgg tgagtaccgc aaccagaggt gcggcttccg cactctctgg
     4981 agcgtaataa aacagcgcgc ccaacacgcg cgccgcgaca gaaaaattat cttgctgtga
     5041 aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc cccgcaccaa tttgttaacc
     5101 tgcaatagcc attcctactg tcatgtgcag gccataaaag agcacacgcc cgatcatctc
     5161 accaacaagt atgaggatta gccccagaac aagtccggca acgtgaggtt cacggcgacg
     5221 aatgagcgga caaagccagc atccaagacc tgcgcataac aataccacac gccagacctg
     5281 taaggaggcg taatctggta ccagtgcgct ggcttgttgc acggatgagt gaatagacgc
     5341 cagagacaaa ccttgcagca caatcacccc ggcacaagcg atcagcgcga gaacgctaat
     5401 gatggcaaat ggtgtggtat taaatgtaac gcgggctgcc cgcagaattg cggcagcaag
     5461 tattggaccg ctcaacaata ccgtcaggaa gaaagccagc gtagtgtaac cgttatgcca
     5521 ggttggcaca gtgtcgattt gatacacgca ggtcatcatc cagacgaaaa tgacgccgag
     5581 cgccatgctg acaagtaacc agagtttccc caacgcttgc ggcattttac cgatgaccgc
     5641 caccagccac catagtccgc caacggcaaa aaatatggaa ccggcagcaa tctcgttact
     5701 cagaccagaa gctccgattc gattcagcga gttaaacgcc cgcagtggcg atcccaggtg
     5761 catgacagag gccataaacc cgatgcccat caacagccag aggaaaaaca tgccacggac
     5821 aatacgctga cggtctgcgt catttttggc ggcgaaccag ccaattccgc taacaatcag
     5881 cgcgcctact acgcattgac ccaatacggt aaagataacc agtggccact catgccatcc
     5941 atttcccatt ttacacttcc tccggattag ccagataacc agtggtatcg ctggtcgggc
     6001 ggctgttggc gttaggtttg ataacgatat tgggttttgt gaaatgcgcg cgcggcagtg
     6061 gcgcgacggc agccagagtg ccgtgcttct ggcgcagctc ttcaattgga ccgaactcaa
     6121 gtgcacgcag cgggcaggat tcgacacata tgggttgttt cccctcggcg acgcgcgaat
     6181 aacaaccatc gcacttcgtc atgtgccctt tttcagcatt gtactgtggc gcgccgtacg
     6241 gacaagccat gtggcagtag cggcagccga tacaaacatc ttcatcaacc accacaaagc
     6301 catcttcacg cttatgcatt gcgccgctgg ggcagacttt tgtacatgcg gggtcatcgc
     6361 aatggttaca ggagatggag agataataag cgaacacgtt ctggtgccag acgccattat
     6421 cttcctgcca gtcgccgcca gcatattcat aaatacggcg gaaactgact tccgggccaa
     6481 gatctttaaa atctttgcac gccagttcgc aggttttaca gccagtacag cggctggaat
     6541 cgataaaaaa tccatattgt gtggtcacgg gctactcctt aaaccttttc gatctggaca
     6601 agattgctgt gcgacgggtt tccctttgcc agcggtgaag ggcggtgaga ggtcagaata
     6661 ttgatactgc cgccgtgatc gacccggtca ccaaacatat ccgctttaag ccaagcacct
     6721 tgcccgatgg cggtaacgcc aggcagaata cgcggagtca cttttgcggc aatcagcatt
     6781 tctccattat tgttaaatac ccgcacggta tcgccatgac ggataccgcg tgcctgagca
     6841 tcaatggggt tgatccacac ctcttgtggg caggcctgct gtaacacatc aatattgccg
     6901 tagctggagt gggtacgcgc tttgtaatgg aagcccgtta actgcagtgg ataggttttc
     6961 cgcaggggat cgtcccagcc atcaaaatct ggggtatacg caggaagggg atgaataact
     7021 tcatcttttt tcaattccca ggtatccgca atcttcgcca gtcgttcaga ataaatttcg
     7081 attttccccg aaggtgtttt caacgggttt gcctgtggat cttcacggaa tgcgcggaaa
     7141 gcgacgtagt gttcttccgg gcattttttc ttaaagatcc cggtcgtttt catctcctcg
     7201 tagtcgggca tctcagggtt acgttccttc gttttcgcat ggagatattt gatccattca
     7261 tgctgactgc gaccttcagt aaaggtttga taaacgtctg gtcctaagcg tttggcgact
     7321 tcactcagca tccagtagat gggtttgcgt tcaaattttg ctgaggttgc gggttgggcg
     7381 aggatcacat agcccatatt ccctgcagat tcatgagaga taaggtcttc ttgctctgtt
     7441 ggcatcaggt cgggcaacag gatatcgcaa tacttagccg aggccgtcat gaagtggtca
     7501 atgccaacaa tcatctcgca cttgctgtca tcctgaagca cctcatgggt gtgattgatg
     7561 tcgccatgtt gattgatcaa tgtgttactg gcgtagcacc ataaaaactt gatggggaca
     7621 tccagttttt cttttccacg aacaccatca cgggtcgcgg tcatttccgt accatggtcg
     7681 atggcatcag tccatgtaaa gacggaaatc tgcgttttaa caggattctc gagcatcggg
     7741 aaccattcta cccccagatc ccagctacct tcgcgtacgc ctgagttgcc gccgtttatg
     7801 ccgacgttac cggtgagaac ggaaagcatg gcaatagcgc gggatgtttg ttcgccgttg
     7861 gagtgtcgtt gtggccccca accctgacaa atataagcag gttttgctga accgatctct
     7921 cgtgccaact ggataatttt ttctgccggg atgctggtga tttttgctgc ccattccggc
     7981 gttttagcta tgccgtcagg cccttcgccc agaatatagg ctttataatg cgcgttacgt
     8041 ggtgcgttgg cgggcagcgt tttttcatcg taaccaacac aatatttgtc gagaaatggc
     8101 tgatcgacca tgttttcagt aatcagtacc caggcaatcg cacaggccag tgcgccatcg
     8161 gtgccagggc gaatgggcag ccattcatct tcacgcccgg cagctgtgtc gttataacgt
     8221 ggatcgatga cgatcatgcg tgcgtttgaa cgttcgcggg cttgctcgac gtagtaagtg
     8281 acaccaccac cgctcatccg cgtttctgcc gggttatttc cgaacataac gaccagtttc
     8341 gtattggcga tatcatccgg gctgttgcca tcattggcac cgaacatata actcattgcg
     8401 gcactgatct gtgcggtact gtagctgcca tagcgactga gaaaaccacc gcaagagttc
     8461 atcagacggt acgggacgtt tgagttggtg atgtttccgc catctacacc tgttccgtac
     8521 aggacatgta cagcttcatt gccgtaatct tttaggatcc gccgaagatt atcactgatg
     8581 gtatccaggg cttcgtccca acttatccgt tcaaatttac cttcaccgcg cttgccgacg
     8641 cgcttcatgg gatatttcaa cctatcagga tgattcatcc gtcggcggat agagcgcccg
     8701 cgtaaacacg cgcgaacctg atgattaccg tagacgtcgt cacctgtcgt atcatactcc
     8761 acccagtaca cggtgtcatc tttcacatgc aaacgtaaca gacagcggct cccgcagtta
     8821 acggtgtagg aactccagac cgctttctct tctaccggag cctctgccgc ccgaaccatt
     8881 tgggaaaatg gcagagtgaa agcactgctt gccagcgcaa ggctgccaag tgcggaggtt
     8941 ttcatcagac ttctacggct gatttcagcc ttcatgagcg cctctgtggt atggattttc
     9001 atcattactc acttattgct tttcaaacaa aatgtcatgc cagaatttat ggttgtagtg
     9061 ggttatattt tttcgatctc gaccagatta gtgtgctgcg ggtttccctt cgccagtggt
     9121 gaagggcgca gagtggttag cgtattcaca cagccgccat ggtcgatttt atcgccagac
     9181 atattggcct cgtgccaggc tccctggccc atagcgctaa ccccagggag aatgcgtggt
     9241 gttactttgg ctggtagccg aacttcgcca cgatggttaa acacccgcac catatcgccg
     9301 gtggcaatcc cacgtttctg tgcatctata gggttgatcc acacttcctg acggcaggct
     9361 gccttcagga catcaatgtt gccgtaggtc gagtgagtac gggatttgta atggaaacca
     9421 aacagttgca gtgggaaggt actacgttca ggggagtccc agccttcaaa ggttgaggca
     9481 taaactggca gtgggcttat cacttcatct ttttccagtt cccaggtacg ggcaatttcc
     9541 gccagcttgc tggaataaat ttcaatctta ccggaaggcg ttttaagtgg atttgcctcg
     9601 gggtcgtcac gaaatgcttt gtaggcgaca aaatgaccat tgggatcttt acgcttatag
     9661 atacccattt ttttcagttc gtcgtaagac ggtaacgccg gatctttggc aagcattttg
     9721 gcgtacagat gttgtaacca ttgttcctgc gtgcgacctt cagtgaactt ttgatagacg
     9781 tcaggtccaa gacgtttcgc gacttcactc aggatccagt aaatcggttt gcgttcgaat
     9841 ttttcgctgg tgacaggctg gaggaaaatg agatatccca tgttaccggc gtagtcgtta
     9901 ggaataatat cttcctgctc aacggtcatc aggtctggca gcagaatgtc ggcat
//
LOCUS       DAZPLM010000149         9770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPLM010000149 DAZPLM010000000
VERSION     DAZPLM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.149"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHVC7_003111"
     CDS             <1..347
                     /locus_tag="ACHVC7_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR2117091.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHVC7_003112"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHVC7_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR2117092.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHVC7_003113"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHVC7_003113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2117093.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHVC7_003114"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHVC7_003114"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR2117094.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHVC7_003115"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHVC7_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR2117095.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHVC7_003116"
     CDS             complement(4344..5309)
                     /locus_tag="ACHVC7_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2117096.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHVC7_003117"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHVC7_003117"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR2117097.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHVC7_003118"
     CDS             6366..6746
                     /locus_tag="ACHVC7_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR2117098.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHVC7_003119"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHVC7_003119"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR2117099.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHVC7_003120"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHVC7_003120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR2117100.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2252 a         2473 c         2548 g         2497 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt
//
LOCUS       DAZPLM010000150         9761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPLM010000150 DAZPLM010000000
VERSION     DAZPLM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.150"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            433..2064
                     /gene="oppA"
                     /locus_tag="ACHVC7_003121"
     CDS             433..2064
                     /gene="oppA"
                     /locus_tag="ACHVC7_003121"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR2117101.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2150..3070
                     /gene="oppB"
                     /locus_tag="ACHVC7_003122"
     CDS             2150..3070
                     /gene="oppB"
                     /locus_tag="ACHVC7_003122"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR2117102.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3085..3993
                     /gene="oppC"
                     /locus_tag="ACHVC7_003123"
     CDS             3085..3993
                     /gene="oppC"
                     /locus_tag="ACHVC7_003123"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR2117103.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4005..5018
                     /gene="oppD"
                     /locus_tag="ACHVC7_003124"
     CDS             4005..5018
                     /gene="oppD"
                     /locus_tag="ACHVC7_003124"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR2117104.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5015..6019
                     /gene="oppF"
                     /locus_tag="ACHVC7_003125"
     CDS             5015..6019
                     /gene="oppF"
                     /locus_tag="ACHVC7_003125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR2117105.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTFKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6072..6401)
                     /locus_tag="ACHVC7_003126"
     CDS             complement(6072..6401)
                     /locus_tag="ACHVC7_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR2117106.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6436..7896)
                     /gene="cls"
                     /locus_tag="ACHVC7_003127"
     CDS             complement(6436..7896)
                     /gene="cls"
                     /locus_tag="ACHVC7_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR2117107.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8039..8212
                     /locus_tag="ACHVC7_003128"
     CDS             8039..8212
                     /locus_tag="ACHVC7_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR2117108.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8267..9520)
                     /gene="kch"
                     /locus_tag="ACHVC7_003129"
     CDS             complement(8267..9520)
                     /gene="kch"
                     /locus_tag="ACHVC7_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR2117109.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2559 a         2277 c         2424 g         2501 t
ORIGIN      
        1 gaaaagatag acatgcttag ccaatctcta tttgattgaa ttgaaagatg tttgttaagg
       61 catggatgca agctatagat tctgatacgg tcaataaaag agaattgctt aacaattttg
      121 caaaatgtat tggcgagtaa gaaccgcatt tggtactttc cgggcaaccg ccagacgatt
      181 ctttattggt aatgagaata attaacaatt aaagagcgtc gcgaaagaat aatgtgtctc
      241 gacaggggag acacagtacg aatcgacata aggtgatcgt ctgaatcacc agaataaata
      301 aagtcggtga tagtaatacg taacgataaa gtaacctgac agcagaaagt ctccgagcct
      361 gtgcagggtc ccaatccggg attacacatg ctggttaata ccagtaatta taatgaggga
      421 gtccaaaaaa caatgaccaa catcaccaag agaagtttag tagcagctgg cgttctggct
      481 gcgctaatgg cagggaatgt cgcgctggca gctgatgtac ccgcaggcgt cacactggcg
      541 gaaaaacaaa cactggtacg taacaatggt tcagaagttc agtcattaga tccgcacaaa
      601 attgaaggtg ttccggagtc taatatcagc cgagacctgt ttgaaggctt actggtcagc
      661 gatcttgacg gtcatccagc acctggcgtc gctgaatcct gggataataa agacgcgaaa
      721 gtctggacct tccatttgcg taaagatgcg aaatggtctg atagcacgcc agtcacagca
      781 caagactttg tgtatagctg gcaacgttct gttgatccga acactgcttc tccgtatgcc
      841 agttatctgc aatatgggca tatcgccggt attgatgaaa ttcttgaagg gaaaaaaccg
      901 attaccgatc tcggcgtgaa agctattgat gatcacacat tagaagtcac cttaagtgaa
      961 cccgttccgt acttctataa attacttgtt cacccatcaa cttcaccggt gccaaaagcc
     1021 gctatcgaga aattcggcga aaaatggacc cagcctggta atatcgtcac caacggtgcc
     1081 tataccttaa aagattgggt cgtaaacgaa cgaatcgttc ttgaacgcag cccgacctac
     1141 tggaacaacg cgaaaaccgt tattaaccag gtaacctatt tgcctattgc ttctgaagtt
     1201 accgatgtca accgctaccg tagtggtgaa atcgacatga cgtataacaa catgccgatc
     1261 gaattgttcc agaagctgaa aaaagagatc ccggacgaag ttcacgttga tccatacctg
     1321 tgcacttact attacgaaat taacaaccag aaaccgccat tcaacgatgt gcgtgtgcgt
     1381 accgcactga aactaggtat ggaccgcgat atcattgtta ataaagtgaa agcgcagggc
     1441 gacatgcccg cctatggtta cactccaccg tatactgatg gcgcaaaatt gactcagcca
     1501 gaatggtttg gctggagcca ggaaaaacgt aacgaagaag cgaaaaaact gctggctgaa
     1561 gcgggttata ccgcagacaa accgttgacc atcaacctgc tgtataacac ctctgatctg
     1621 cataaaaagc tggcgattgc tgcctcttca ttgtggaaga aaaacattgg tgtaaacgtc
     1681 aaactggtta accaggagtg gaaaacgttc ctcgacaccc gtcaccaggg cacttttgat
     1741 gtggcccgtg caggctggtg tgcagactac aacgaaccaa cttccttcct gaacaccatg
     1801 ctgtcgaaca gctcgatgaa taccgcgcat tataagagcc cggcctttga cagcattatg
     1861 gcggaaacgc tgaaagtgac tgacgaggcg cagcgcacag ctctgtacac taaagcagaa
     1921 caacagctgg ataaggattc ggccattgtt cctgtttatt actacgtgaa tgcgcgtctg
     1981 gtgaaaccgt gggttggtgg ctataccggc aaagatccgc tggataatac ctacacccgg
     2041 aatatgtaca ttgtgaagca ctaatggcaa tacgtggggc aggagtgtcc tgctccacgg
     2101 tgtctgattt ttatcgcatt acagaaggca caggccagaa ggtagggcaa tgttaaaatt
     2161 tattctacgt cgctgtctgg aagcgattcc gacgctattt attcttatta ctatttcgtt
     2221 ctttatgatg cgcctcgcgc cgggaagccc ttttaccggc gaacgtactt taccgccgga
     2281 agtgatggcc aatatcgaag cgaaatatca tcttaatgat ccgatcatga cacagtattt
     2341 cagctacctg aaacaactgg cgcacggtga tttcggtcca tcgtttaaat ataaagatta
     2401 ttcggtcaat gacctggttg catccagttt tcccgtttct gccaaactgg gagccgccgc
     2461 atttttcctt gcggtaatac tgggtgttag tgctggcgtt attgtcgcat taaaacaaaa
     2521 caccaaatgg gactataccg tgatggggct ggcaatgacc ggggttgtta tccccagttt
     2581 tgtggttgcg ccattattag tcatgatatt tgcgatcatt ttgcattggc tgccgggcgg
     2641 tggctggaat ggtggggcgc ttaaattcat gatattgccg atggtggcgt tgtcactcgc
     2701 ttatatcgcc agtattgcgc gtattacccg aggctctatg attgaagtat tacactccaa
     2761 ctttattcgt actgcccggg cgaaagggtt acctatgcgg cggatcattt tacgccacgc
     2821 attaaaacct gctctgttac ccgtgctctc ttatatgggc cccgcatttg tcggcattat
     2881 taccggttct atggttatcg aaaccattta tggtttgccg gggattggac aactgttcgt
     2941 taatggcgca ttgaaccgtg actattcctt agtgttaagc ctgaccatcc tggttggtgc
     3001 tttaaccatt ttgtttaatg ccattgtcga tgtgctatat gcggttatcg acccgaaaat
     3061 ccgttactga tactggagct cgcgatgatg ttaagtaaga aaaacagcga gacgctggaa
     3121 aatttcagtg aaaagctgga ggtcgaaggg cgcagcttgt ggcaggacgc acgtcgacgt
     3181 tttatgcata accgtgcggc ggttgccagt ctgatagtgc tggtgctgat cgcgttattt
     3241 gtaatcctgg caccgatgct ttcgcagttt gcctatgacg atactgactg ggcgatgatg
     3301 tccagcgccc cggatatgga gtccggtcac tactttggta ctgactcatc cggtcgcgac
     3361 ctgcttgtgc gcgttgcgat tggcgggcgt atctcactca tggtcggtgt tgctgcggca
     3421 ctggtggcag tggtcgtggg gacactttac ggttcgcttt ccggttatct gggcggtaaa
     3481 gtggattcgg taatgatgcg tctgctggaa atcctcaact ccttcccatt catgttcttc
     3541 gtcattttgc ttgtgacctt tttcggtcaa aacatcctgc tgattttcgt ggcgattggc
     3601 atggtttcct ggctggatat ggctcgtatt gtgcgtggac aaaccctgag tctgaagcgc
     3661 aaagagttta ttgaggcggc acaagttggc ggtgtatcga catcgggcat tgttattcgc
     3721 cacattgtgc cgaatgtact cggtgtggtg gtggtctacg catcacttct ggtgcccagc
     3781 atgatcctct ttgaatcttt ccttagcttc ctggggttgg gtacgcaaga gccgttaagc
     3841 agctggggtg cattgctgag tgatggcgcg aactcgatgg aagtctctcc atggttattg
     3901 ttgttcccag cgggattcct cgtggtgacg ctgttttgtt tcaactttat cggcgatggc
     3961 ttgcgtgatg ctctcgaccc gaaagatcgt taaggagtgc agccatgagc gtaattgaaa
     4021 ctgcaactgt gccgctcgca caacaacagg ctgacgcact gctgaacgtg aaagatttgc
     4081 gtgtcacctt tagtaccccg gacggcgatg tcacggcggt caatgatttg aatttttctc
     4141 tacgtgccgg agaaacgctg ggcattgtag gtgagtctgg ttcgggtaaa tcgcaaactg
     4201 catttgcgtt gatgggcctg ttggctgcca acggacgtat tggcggatcg gcaaccttca
     4261 atgggcgtga aatcctcaat ttgccagagc gtgaactcaa taaactgcgc gctgaacaaa
     4321 tctcaatgat ttttcaggac ccaatgactt cgttgaatcc ctatatgcgc gtcggtgagc
     4381 agttgatgga agtgctgatg ctgcataaga acatgagcaa agctgaggcg tttgaagagt
     4441 cggtgcggat gctcgatgcg gtaaaaatgc cggaagcacg taaacgcatg aaaatgtacc
     4501 cgcacgaatt ttctggtggc atgcgtcagc gagttatgat tgcgatggca ttgctatgtc
     4561 gacctaagct gctgattgcg gatgaaccca ctactgcgct ggacgtcacc gtacaggcgc
     4621 agatcatgac gctattaaat gaactgaagc gggaatttaa taccgccatc attatgatta
     4681 cccacgatct tggcgtggtg gcggggatct gcgacaaagt gctggtaatg tacgccgggc
     4741 gcacgatgga atatggcaac gcgcgcgatg tcttttatca acccgttcat ccttattcta
     4801 tcggtttgct caacgcggtg ccgcgtctcg atgcggaagg tgaaacaatg ttgaccatcc
     4861 ctggtaatcc gccaaacctg ctgcgattac cgaaaggttg cccgttccag ccacgttgtc
     4921 cgcatgcgat ggaaatttgt agtagcgcac cgccgctgga agagtttacg cctggccgtc
     4981 tgcgtgcttg ctttaaaccg gtggaggaac tgttatgaat gctgtaaccg aaggaagaaa
     5041 agtcctcctc gaaatcgccg atctgaaagt gcactttgaa atcaaagatg gcaaacagtg
     5101 gttctggcaa ccgccgaaaa ctttcaaagc cgtcgatggt gtcacgcttc gcctgtatga
     5161 aggggaaaca ttaggtgtgg taggggaatc gggatgcggt aagtccacct ttgctcgcgc
     5221 catcattggc ctggttaaag cgaccgacgg tcatgttgcc tggttaggta aagagttgct
     5281 gggcatgaag cccgatgaat ggcgtgccgt tcgcagtgat attcagatga ttttccagga
     5341 tccgttggca tcgctaaacc cgcgtatgac catcggcgag atcatcgctg aaccactgcg
     5401 tacttatcat ccgaaaatgt cacgccagga agttcgcgag cgcgtgaagg cgatgatgct
     5461 gaaagtcggg ttattgccta acctgattaa ccgctatccg catgagttct ccggtgggca
     5521 gtgccagcgt atcgggattg ctcgtgctct tattcttgaa ccgaagctga ttatctgcga
     5581 tgagccgatt tcggcgctgg acgtgtcaat tcaggcgcag gtggtcaacc tgctccagca
     5641 gctgcaacgt gagatgggat tgtcattaat ttttatcgct catgacctgg ccgtggtaaa
     5701 acacatttcc gatcgtgtgt tggtgatgta tctcggccat gcggtagaac tggggaccta
     5761 tgatgaggtc taccacaatc cactacatcc ttacaccagg gcattgatgt cggcagtccc
     5821 catacctgat ccggatctgg agaagaacaa aaccatccag ttactggaag gggaattacc
     5881 gtcgccgatc aacccgcctt ccggttgtgt tttccgtacc tgttgcccga ttgccggtcc
     5941 ggagtgcgcc aaaacacgtc ctgtgctgga agggagtttc agacacgccg tttcttgcct
     6001 gaaagtcgat ccgctttaaa cacaataagg gctgacagtt gtcagccctt tttcacgccg
     6061 aaagcgatta tttattcccg ccagatgata tggcaaagtt tgtgatcttt ttcgcggcat
     6121 aacagaatgc gggcgaaaac atcgttgatt tcaccatcct cactgtccgc cagaccaatc
     6181 accacttcgg caaaaaagtc cgggttcaaa tcgaaatcaa catgttcctg ccagtcttcc
     6241 gccggatcga ataactccgc gccgccgcgc tcttcaaact gaagattgaa cagcagaaca
     6301 tcagctgggt cgagattgtc ggcagccagt tcgagaaaaa tatcgtaggc ctgctcaagc
     6361 gtttcatctt cagtcaggcg attgtttaga tccatatcca tagtcactac ctgtttaacc
     6421 tctgttggcg acgttttaca gcaacgggct gaagaagtaa aacagtcgct cggcgacacg
     6481 ttgccataat ggacgtttta gccataaacg ggcatcgagc agacgtgaac gcgaaatata
     6541 atcgtcctga acggcggcaa ggtctgcacc aaaaccttta tcgtcgattg ccagggtaat
     6601 ctcgaaattc agccacagac tacgcatatc aaggttaacg gtgccaacca gacttagttc
     6661 gccatcgacc agcacgctct tggtatgcag taacccgcct tcaaactgat aaattttaac
     6721 cccagcagca agcagttccg taaagaatgc gcgactggcc cagccgacca gcatcgagtc
     6781 attttttcgc ggaaggataa tactgacatc caccccgcgc tgcgccgccg tgcaaatcgc
     6841 atgaagtaaa tcatcgcttg gcacaaagta gggcgttgtc atgatcaaat attcacgcgc
     6901 cgaataagcc gcagtcaata atgcctggtg aatgagatct tccggaaagc cggggccaga
     6961 agcaattgtg tgaatggtgt gaccgctggc ctgttcaaac ggcataatat tgacatctgg
     7021 tggtggcggc agaatacgtt ttccggtttc aatctcccag tcgcaggaat aaataatccc
     7081 catcgcggtg gcgatggggc cttccatacg cgccatcaga tcaatccatt gccctacgcc
     7141 cgcatcttgt ttgaagtagc gaggatcgac catattcatg ctgccggtgt acgcgatgta
     7201 attatcgatc atgatcatct tgcgatgttg gcgcaggtcc atacggcgta aaaacacacg
     7261 catcagattg acctttaagg cttcgaccac ttcaataccg gcattacgca ttagctcggg
     7321 ccacgggctg cggaaaaaag ccacactccc ggcggagtcg agcatcaatc ggcaatgaat
     7381 accacgccgt gcagccgcca ttaatgattc agccacctgg tccgccatgc cgccgggctg
     7441 ccagatataa aacaccatct caatattatg gcgcgcgagc tggatgtcgc ggattaacgc
     7501 ctgcatcaca tcatctgact cggtcatcag ttgtagctga ttccctttga ccccagcgat
     7561 cccctgacga cgctcgcaaa gcttgaataa tggcgcagcg acactgctat tttcttcggc
     7621 gaagatatgc ttacaggctt taaggtcgtt aagccatttt gcggtggaag gccacatcgc
     7681 tctggcgcgc tcagcgcggc gtttgcctaa atggagctcg ccaacggcaa gataggcaat
     7741 aattccgact aacggcagaa tgtaaataat caacagccag gccatcgcgg agggaactgc
     7801 gcgtcgtttc attagaatgc gtaaagttac gcctgcaatg agcaaccagt atcccagaat
     7861 ggccaaccaa ctcaccaacg tataaacggt tgtcatagat tagaaatcct tttgaaagcg
     7921 cattgttatg agtttacgca tatggattca tctggcaaat aaaaacgcgg gaaaagcact
     7981 ggtcagcgtc gggggtgggg gtataatgac cattctgtta ttgcatagag tagttaacat
     8041 gaagcggagt agaacggaag tggggcgctg gcgcatgcag cgtcaggcta gccgacgtaa
     8101 atcgcgttgg cttgaggggc aatcgcgccg aaatatgcgt atccacagca tcaggaagtg
     8161 cattctaaac aaacagcgta actcgttatt gtttgcgatc tacaatatct aaatatactg
     8221 ggcaccattg cggtgcccaa taagtttact ctttcgtctt taaactctat ttttgcactg
     8281 attctttact gtcagctttc gtttcttgtt catcgttatc gctgaaaatg ccatgaccgg
     8341 agttcaacaa cattgaaacg agcatatcat tattaatctc ttcaccattt aaaactcgcg
     8401 ccagaatttc gctgccaaac agttgcggtg agagaatgat atccggatgt accatcttaa
     8461 tcttattcag gtttttgcta tcactgacgg cgagaactgt tttgacatca ctgctcatat
     8521 cttttgccga gagtacaaca aacgcgttat ctgcatcgtt atcactcagc gccagaatgg
     8581 cccggcaacg atcgattccc gctttcttta atactgaact gtcattactg tcaccgggga
     8641 taacatcagc gttatcgcct aagcgttgct caagttgctt gatatcatct tcaggcaagt
     8701 tgctgataac cgttacgttt tgtccgcgtt gattcagttg caggatcgta ttgatggcga
     8761 gaatcgaatg tccgcaaaca ataaaatgat ctttacgatg cattgtatga ttgtttcctt
     8821 ttacaagttt gttgaatccc ccgcggataa gcggaccaaa aattgaggtc atagatgtgg
     8881 caaaaacggt aatgccggaa ataatgaccg aaatagtgaa caatcgtgct gattcagaaa
     8941 cagggacaat atcgccatag ccgacggttg acatggtttc tatcgaaaaa tagaacgcgg
     9001 tcatcaaact ttctattcgc ggattaaaac cttcgcttaa ataaagcgca ccgtaggttg
     9061 agtaaaacag taatgtcgtg aaactaataa atgcaaaaat tgtcccggct gcggcgctac
     9121 tgtgggagaa gtctttgcgc agtatcagca aaaacaccag cgtaaaaatg caaaatccaa
     9181 tactaaattt cagccaggga taaaaatgca gggtgtaaat tagcgctatc aacaacaaaa
     9241 tgatactgat tgcccatgcg agctttgccc ggaacagtaa gccaatggag ttcaggacaa
     9301 gaaagacgcc gagcataaat agtggggcgt ttgccagcaa ggagagatcc aactctgaaa
     9361 atgctttgat atggaaaata tcaagcaaat tgaccgacat accatagatt gttttaaaaa
     9421 taagcaatcc atttaaaaag acggccagcg cgagaatgtc gtgccgaagg gtcacccata
     9481 aatttgttgc tgtttgtttg aatgtagccc agtgactcac tgatacctct gtaaccacaa
     9541 ttttgtttaa ttatcttagc gtgcacattt tctgaacata catgcagcgc gtgaatgtgt
     9601 taaacaatag tagatgactt tatccgcagt aatcaaagga tttgagcgag caactgtacc
     9661 tcaggaaagg tcatttttcc tgaatatgct cacatcatat aaagaaatac agataaagtt
     9721 attatctgct tgtggtagtg aatgcactga ccggctataa g
//
LOCUS       DAZPLM010000151         9704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPLM010000151 DAZPLM010000000
VERSION     DAZPLM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.151"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHVC7_003130"
     CDS             complement(<1..143)
                     /locus_tag="ACHVC7_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117110.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            221..1315
                     /gene="ddlA"
                     /locus_tag="ACHVC7_003131"
     CDS             221..1315
                     /gene="ddlA"
                     /locus_tag="ACHVC7_003131"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR2117111.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1339..1551)
                     /locus_tag="ACHVC7_003132"
     CDS             complement(1339..1551)
                     /locus_tag="ACHVC7_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR2117112.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1811..2119
                     /locus_tag="ACHVC7_003133"
     CDS             1811..2119
                     /locus_tag="ACHVC7_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR2117113.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHVC7_003134"
     CDS             complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHVC7_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR2117114.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHVC7_003135"
     CDS             complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHVC7_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR2117115.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4857..6014
                     /gene="ampH"
                     /locus_tag="ACHVC7_003136"
     CDS             4857..6014
                     /gene="ampH"
                     /locus_tag="ACHVC7_003136"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR2117116.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHVC7_003137"
     CDS             complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHVC7_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR2117117.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6726..9614)
                     /locus_tag="ACHVC7_003138"
     CDS             complement(6726..9614)
                     /locus_tag="ACHVC7_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR2117118.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNNKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2491 a         2403 c         2339 g         2471 t
ORIGIN      
        1 gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc gttcatcatt
       61 tttgtgatct cgcgcacatc tttgagataa cgcactttct tcaccagtac gttgcacgct
      121 ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt tttgtcgtgg
      181 taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg
      241 aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat
      301 tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg
      361 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat
      421 tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg agcatcagct
      481 tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc cgattgtcca
      541 cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt
      601 tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca ccaaacgtct
      661 gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg ctaatcgtca
      721 caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc
      781 taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc
      841 cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg
      901 tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct gtggcgagat
      961 cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag atggcgcgaa
     1021 agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg cgattgccgt
     1081 tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc
     1141 agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat
     1201 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat
     1261 tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt
     1321 taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac gacgaataat
     1381 caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca
     1441 accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata taaggccaat
     1501 ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca tggcagctcc
     1561 gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg tagacaacat
     1621 tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtaactcac aacgaagatt
     1681 cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga caatctaaat
     1741 gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc
     1801 ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa
     1861 tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc
     1921 tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gtttaatgca
     1981 ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg
     2041 gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat ggctgcactg
     2101 gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc cttatctggc
     2161 atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc
     2221 gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc
     2281 aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg
     2341 tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg
     2401 ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt
     2461 tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta
     2521 ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca
     2581 aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta agtaccaaac
     2641 cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat tttccacgga
     2701 taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc
     2761 accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat cagatcgtca
     2821 aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg
     2881 ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat gtgcatccgt
     2941 tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct
     3001 gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc
     3061 ggtgccagcc cctgactttc aaaggtgatg gcgagatctt tcgcccaggc atcgcggtct
     3121 ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc tttttcgccc
     3181 tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac cgccaacaat
     3241 gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg
     3301 gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag
     3361 atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga
     3421 acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca
     3481 attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg
     3541 gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg tacggcgcta
     3601 aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc
     3661 tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc
     3721 cctaccactg ccagccatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga
     3781 ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag cgttaccagc
     3841 accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc
     3901 agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc ttcgatatga
     3961 cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacaca
     4021 tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag cgcaatcccc
     4081 agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc
     4141 gcggtttgaa tcaaatcata gaacggtgca taccaggcgt tgacggcgac cccgacttcc
     4201 accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa
     4261 cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac aatgtagtaa
     4321 gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg
     4381 gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa gataacggca
     4441 atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa aaaagactta
     4501 aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg
     4561 accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag
     4621 ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag attgtttaca
     4681 taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg
     4741 tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca
     4801 tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga
     4861 aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg
     4921 cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata
     4981 ttttttacgg cagcggtgca acggggatgg cgctggttgt tatcgacggt aaccagcgcg
     5041 tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg
     5101 tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg aaattgctcg
     5161 accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg
     5221 tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat accagcgccc
     5281 tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg
     5341 agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc agtcaggcgg
     5401 cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg tccggcaagc
     5461 cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct
     5521 acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt
     5581 gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga
     5641 tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc
     5701 gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc atggatgtcc
     5761 ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc
     5821 cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg gcgatgatcc
     5881 cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg cgctttaaaa
     5941 atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa ccgttggtta
     6001 tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta
     6061 ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc
     6121 gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca
     6181 ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt atgcccaatg
     6241 agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct
     6301 cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag atgatatccc
     6361 gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc
     6421 agccccataa tataaggggc ctgggtaata ccgataagta cgttttcttc tctacgcaga
     6481 gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact ggaaaatata
     6541 acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc
     6601 ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg
     6661 cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct tcggagtgaa
     6721 gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc tacgtcacca
     6781 ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct gaactgagta
     6841 cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc accgttcaca
     6901 tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg agtcagagcc
     6961 tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata acgcatgctg
     7021 tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc accagactgg
     7081 aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag tttgaagtcg
     7141 taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc aacgtaagta
     7201 ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa gctaccatca
     7261 acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg gctgtcttga
     7321 tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc cgcaccgacg
     7381 atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat gccgttaaca
     7441 tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa gtagcttacc
     7501 catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt accaacggtg
     7561 tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc catgttagcg
     7621 tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc agcctgatag
     7681 gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt tttggtatca
     7741 tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc agtggagttg
     7801 tcgatacgaa ctttgttgtt accggttgca gctacaacgt tgtctgcaga gctgttgtca
     7861 acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc atcagagttc
     7921 atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg atcgttgacg
     7981 aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc gtaaacatta
     8041 ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc aaatacgctg
     8101 gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac ggtaatggta
     8161 ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa gttaacggtt
     8221 gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc cattgaagaa
     8281 ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac gttaacctga
     8341 gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc ccaatcagaa
     8401 ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt atcaacacga
     8461 ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc aacgtcgtag
     8521 aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac ccatttggtg
     8581 ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca accgccatga
     8641 gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt agcgttccag
     8701 gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac gttgttgacc
     8761 atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc agcaggagat
     8821 gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg atctgattgt
     8881 tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat gttgttgtcc
     8941 cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac caaagtaata
     9001 cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag agtcacagcg
     9061 gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt accgtctgca
     9121 tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt agaattagcg
     9181 atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc gctaccgttg
     9241 gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg aacaattgag
     9301 ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag ataataagta
     9361 gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc atcagctgcg
     9421 tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt gctgtcaccc
     9481 caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt agctgcatta
     9541 gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac aagtttcttt
     9601 ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc atcaattatc
     9661 aatgcataga aaacatatta tgtttcgcgc taacgattat gctg
//
LOCUS       DAZPLM010000152         9676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPLM010000152 DAZPLM010000000
VERSION     DAZPLM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.152"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1712
                     /locus_tag="ACHVC7_003139"
     CDS             <1..1712
                     /locus_tag="ACHVC7_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR2117119.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1713..3362
                     /locus_tag="ACHVC7_003140"
     CDS             1713..3362
                     /locus_tag="ACHVC7_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR2117120.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3367..4143
                     /locus_tag="ACHVC7_003141"
     CDS             3367..4143
                     /locus_tag="ACHVC7_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR2117121.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="ACHVC7_003142"
     CDS             complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="ACHVC7_003142"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR2117122.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5432..6754)
                     /locus_tag="ACHVC7_003143"
     CDS             complement(5432..6754)
                     /locus_tag="ACHVC7_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR2117123.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="ACHVC7_003144"
     CDS             complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="ACHVC7_003144"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR2117124.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="ACHVC7_003145"
     CDS             complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="ACHVC7_003145"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR2117125.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="ACHVC7_003146"
     CDS             complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="ACHVC7_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR2117126.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2485 c         2605 g         2232 t
ORIGIN      
        1 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga
       61 acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca
      121 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag cagaaaaatg
      181 tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggctagc aatatccggc
      241 tacaaagtag cgaaacgccg caggagattt tccgcaacaa tcttgatgcg ttagtcgatg
      301 gaccatgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
      361 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
      421 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
      481 atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg caggccagcc
      541 aggcgctcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac agttatgccg
      601 agcaggcgga taacatgccg ttattgcatc gggcgctggt gctggcgtgg gcacaggaga
      661 tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
      721 ctaaagatga agatttctct gaggaagaca tccgcgatat caatgacagc ctgatcctcg
      781 atacaccgga atctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
      841 aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg tgggataaag
      901 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg
      961 ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact attgagcgcg
     1021 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
     1081 cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaagac tggcgttggg
     1141 ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
     1201 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
     1261 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
     1321 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
     1381 agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg ggagccgacg
     1441 ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa cagcaggggc
     1501 aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
     1561 aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg
     1621 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt gttgcggcag
     1681 ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc gtctggctat
     1741 tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg
     1801 gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
     1861 tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt tacgcctggc
     1921 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
     1981 tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta cgttcctgtg
     2041 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
     2101 aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca gaaacctcgg
     2161 taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac aaagctcagg
     2221 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta
     2281 gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa atccgccagg
     2341 gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc agtggcacca
     2401 gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca gtgccgatgc
     2461 aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa
     2521 tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg cgctatcgtg
     2581 tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg accttactga
     2641 gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac gttgccagag
     2701 ttctggatcg cgaggcgaaa agtacgccac ctgaggcagc gaaagccatg accgtggcga
     2761 tacgtacttt tttacagcaa aacgccaatc gggaaggtga ttgcctgacc atccccgaca
     2821 gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga acgatgaccg
     2881 cttggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc agtcgcgcta
     2941 ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg gagcgttacg
     3001 accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga gctccacgct
     3061 caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc
     3121 ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt
     3181 tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt cgtaacttct
     3241 ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc tttgatggtt
     3301 atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta ttaatggact
     3361 aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc tgtcgctcac
     3421 agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca tccggcagag
     3481 ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt caacatggca
     3541 gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc tgcggcgggt
     3601 aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag aattgaatcc
     3661 gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt gcaggtcatc
     3721 agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgtcggg tataggaacg
     3781 attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga tctcgatctc
     3841 catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct gaaaaacagt
     3901 ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc catgccagca
     3961 ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag cgaaacggaa
     4021 ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga gaaacaggaa
     4081 acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag ttttgactgg
     4141 tgattaatgg ggttaccttc gccgtcgatg ttcaagaaaa cgcccgataa ctttcgctat
     4201 cgggtgtttt tattgattaa ttcaaccgtt caatcaccat cgcaattccc tggccgccac
     4261 caatgcacag tgttgccagc cccagcgttt tatcgcgtgc ctgcatggca tgtaatagtg
     4321 tgaccagaat acgagcacca ctggcaccga taggatgccc gagcgcgatg gccccgccgt
     4381 tgacattcac tttctcagaa tcaaagccca gggttttccc aacggcgagg aactgtgcag
     4441 caaatgcttc attagcttcg atgagatcaa tgtctgccag ttgcagcccc gccagttgta
     4501 acgctttttg cgtggcaggt actggcccca tacccatcaa tgcgggaggc acgccaccgc
     4561 tggcataact tttaatgcga gccagggggg taaggcctgc tgccagcgcc gcagattctt
     4621 ccataatcac cagagcggca gcaccgtcgt taataccaga cgcgttccca gcggtgactg
     4681 ttcctgcttt atcgaaggcc gggcgcaatg cacctaacgc ttcagccgtt gaattcgctt
     4741 tcgggaattc gtcttgactg aagacgaagg ttttcttccg agtgacaaca tttaccggga
     4801 cgatttcggc tgtaaaagca ccggactcaa ttgcggctgc cgctttacgc tgtgaatgta
     4861 gcgccagttc atcctgcatt tcacgggtaa ttccgtactc tttagccacg ttttcggcgg
     4921 taatccccat atgataacca tgggtggcgc acatcaggcc atcgcgcagg attacgtcat
     4981 aaacctgtcc gtctccaaga cgataaccag agcgtgcttt tgcatcgagt aagtagggcg
     5041 ctaaactcat attttccata ccccccgcca caatgctctt cgcctgacct gcctgaattg
     5101 cctgggcggc aagcgccaca cttttaagac ccgaaccaca cactttattg accgtgaatc
     5161 cgcacaccgt ttctgccagc ccgcttttta acagtgcctg acgtgccgga ttttgcccca
     5221 acccggcttg caacacgtta cccataatca cttcatcaat gtgtagtgaa tcgatttttg
     5281 cacgttcaat ggcggcttta attactgtcg cccccaggtc gatggcgctg gtggaagcga
     5341 gtgaaccgtt aaaactaccg atagcagtac gtaccgcact gacgatgaca caatttttca
     5401 ttttatattc cttctgtctg tagggttgcc gtcagaacag cgttaaacca atgacgaaaa
     5461 tgacgccgga gaagagcagg gcagtgatgc aatagcccat gatgtcgcgg acaccgagtc
     5521 cggcgattgc cagtgctggc agcgcccaga atggttgttc catgttcatc cattgctcgc
     5581 cgtaggcgat cgccattacc gatttaccga gatcagcgcc cagcgcctgg gctgcgggta
     5641 tcacgaaagg tccctgaata acccagtgac cgccgccaga cggaacggcg aagttaatca
     5701 gtgcagaact aaaaaaggtc attaccggga aggtgtcttt gttcgcaaca ttgatgaaga
     5761 attcggtaat gagtccaccc agaccagaat gctccatcat cagttggatc ccagcgtaga
     5821 aggggaattg caccagaata ccggcagtac tgcgtgctgc cgcgctgata gcacgcatat
     5881 aagccattgg cgttttatgt agcaacagac ccgcaatcat aaacatcagg ttgacggtat
     5941 tgatggtgat gttgaagcca tgttcgctga agtacatcgc caggtaagcg ataccgagtg
     6001 cgccgatgat caacgtcaga atgcggcttt cttccagtcg ctcggatggt ggggcatctt
     6061 tcggtagctg cttttgaaaa tcagcctctt ccatgagtag ttccggatcg atactcacca
     6121 cgtcagacgg ttttggcatc atcatgcggg tgataaatgg catcaccaca atcaacgtca
     6181 cagtgatgaa aatgttaaaa ccactgaaca gagtatcgcc caccgggatc agcccggcga
     6241 tatgctcaac cgggttgccc ggtgttgcag ccaacagagg cattgatcca gagaagccgc
     6301 caccccaggt gagaaaacca atgtaggcgc aggcaatgag caacggataa tcagaaccgg
     6361 ggactcgccg ggcgacttca cgggcaaaca ttgcgccgac aaccaaacca aatccccagt
     6421 tgatgacaca agcgactgaa ccgaagaaag tgaccagcat gacgccctgt acgggcgtct
     6481 ttgcggcgga ggcggcagta cgcagcaaac ttttcaccgg agcagagctg gcaagggcat
     6541 gaccggtaac gatgataagc gccatctgca taccaaacgc cagcaagttc cagaaaccgt
     6601 caccccacat tttcaccatg ctgatcggcg tttgtggtgt taaccaaagc gcgatcacga
     6661 atgttagcaa tgtcagcaac atggcaaaga tcagtggatc gggaagccat cggctgacaa
     6721 aacgcgtcat aaaacgcgat atgcgaccaa tcataaatca ccccgttgcg tattcagatc
     6781 ggcggcgact tcaaaccgag cttctgtttt ggcacgcacg gtggctaaat cacacccgtc
     6841 ggcaatttcg gtgagccaca ttttgccgtc aataaaacga aagacagcca gttcagtaac
     6901 cagcatatgc accgcatgtt gcgcagtgag tggcatggtg cagctgcgca aaatttttgc
     6961 tgaaccatct ttggcgcaat gttccatggc gatgatcact ttgcgcgacc cggtcaccag
     7021 atccatcgcg ccacccatac cgggcaccat tttcccaggc actacccagt tcgcgaggtt
     7081 tgcttcttcg tctacttgca aaccgccgag cacgcaggca tcaatatgac cgccacggat
     7141 tagcgcaaat gacatggcgc tatcaaacat ggctgcaccg ggtaaaacac cgcacggttg
     7201 cccgccagcg ttcaccagat ctggatgcgc tgtcgtgacc gggcctaaac cgaggaagcc
     7261 gttttccgat tgcagagtga tatgaatacc ctccggtaaa taattggcga ccattgtggg
     7321 taaaccgatc cctaagttaa cgatgtcacc atcacgaagc tcttgcgcca cacggcgcgc
     7381 aatacgttgt ttcgcatcca ttatttgctc tcctgtgaaa cgatgatgtg gtcgataacg
     7441 gcaccagggg tgacaatatg gtcaggttgc agctcgccgg tttcgaccag ttcatctggc
     7501 tctaccagcg tgatatcagc cgcaagggct atcagggggt taaagttgcg ggcgctaagt
     7561 tgataggtca ggttgccaag tgtgtcgcaa cgatgagcgc gaattagcgc caggtcggcg
     7621 cgcagtgggc gttcgagcag ccaggtttta ccgtcgagtg tcagtgtctg tttgccttcc
     7681 tctacgacgg tgccgacacc cgttggggtg agaaaaccac caagtccagc tccaccacag
     7741 cgaatttgct cgattagcgt accttgcggc accagaacga cgtccatctc accagatatc
     7801 atgcgccgac ctgtttccgg gttggtgccg atatgtgaag caatcacttt gcggactcga
     7861 ccattgacga tgagcggacc gatgccggta tcaacaaacg cggtatcatt ggctatcaat
     7921 gtcaggtcgc gaacaccaga ttccagtaat gcttcaacca ggcgggatgg agtgccaatc
     7981 cccataaatc cgcccaccat gatggtcatg ccgtcacgaa agaagccggt ggcgtcttgt
     8041 aatgtcatca attttgtttt cattttttat ccctcttgca tacaccgttg tgggtacagt
     8101 caggcaatag caaggggagt gccagaatga gattattgga tttagttatt taactaattg
     8161 aatatgaagg gataaattgg atggggattt ataaggagaa tgagcggcta tgcagaaaat
     8221 tgcgcactgt gcaaatttct gcatagcaag ttttggtgtt atacatccgc cggatcgata
     8281 ccgtattcct ggagtttata catcaatgca cggcgactga tgcccagcat taaagcagtg
     8341 cgggttcggt ttccttcttg ttgttccagc acttccataa tgatgcgttt ttcgacgcgt
     8401 ttaatttcct cttttaaatt acgctcaccg acagaggctg tttttacctc gccagcatta
     8461 cagactggct gacgaatctg tggcggaaga tcctcagaaa aaatgatcgg gcctgaattc
     8521 atcacgacgg cgcgttcaat aacgttagaa agctctcgaa tatttcccgg ccatgaccag
     8581 gcggtaagca gtgacattgc catcggatcg atgtcgataa tatcgcgctg attctcacta
     8641 ctgaattttt gcaaaaagtg attagctaac agggaaatat cttcccggcg atcgcgcaga
     8701 ggcggcagta ttaaatgaat aacgttaagg cgataaaaga gatcttcacg gaaggtgcct
     8761 tcttttacca ttgcctgcaa gtcacggttg gtggcagcaa tgatgcggat atcaactttt
     8821 atggtctgat ggccgccaat ccgttcaaat tcccgttcct gtagaatgcg tagtaatttg
     8881 gcttgtagta ccagcggcat ttcgccaatt tcgtcgagga gcagagtacc ttcgttggct
     8941 cgttcaaata atccctgacg caaggtttgt gcaccagtaa atgcaccttt ttcatgacca
     9001 aacagttcac tttcgagcaa cgattccggc agcgccgcgc agttgacttt aatgaacggc
     9061 ccctttgccc gccgcgaatt gtagtgaatc gctctggcaa tcaactcttt cccggtgccg
     9121 ctttcaccgc taatcaagac gctggcctga gaaagggcaa ttttggcggt gtctttgcag
     9181 atgtccatca tcgccgggct gttggtgaga atgtgccccc attgccagct ggcgctcagt
     9241 gcctggtgca gatgacggat ctcttttttc attgactgga gttgtaaagc gcgctgaacg
     9301 attaaattca actcatcgag atcaaacggt ttaataacat agtcgaaggc tccgcagcgt
     9361 agcgcttcga cggcggtttc cacttccgca taggccgtca tcagaataac gggtgtccgg
     9421 gtctcatggc tgcgcatctc ctttagtgcc ttgatgccgt ccatctctgg catgcggata
     9481 tccatcaaca ccacatcagg gtgaatatcg gcaaacaggt gtaatgctgt gcgtccgttg
     9541 ttcgcacaat gtgtttcgaa tccttgtagt gcaaaagcgg tgctcagcat acggcgaaca
     9601 ttatcttcat catccacaat aaggatgcga ttaatagcag tcatacagtc tgatttccct
     9661 gcgggttgat cggtaa
//
LOCUS       DAZPLM010000153         9649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPLM010000153 DAZPLM010000000
VERSION     DAZPLM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.153"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHVC7_003147"
     CDS             complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHVC7_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR2117127.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHVC7_003148"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHVC7_003148"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR2117128.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="ACHVC7_003149"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="ACHVC7_003149"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR2117129.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="ACHVC7_003150"
     CDS             complement(5701..7017)
                     /locus_tag="ACHVC7_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR2117130.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="ACHVC7_003151"
     CDS             complement(7007..8764)
                     /locus_tag="ACHVC7_003151"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR2117131.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9649)
                     /locus_tag="ACHVC7_003152"
     CDS             complement(8780..>9649)
                     /locus_tag="ACHVC7_003152"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR2117132.1"
                     /translation="FALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVI
                     SGPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIP
                     LAGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALL
                     VLFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLL
                     TPLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFT
                     APVFFYFIRYCCY"
BASE COUNT         2449 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcgaa
//
LOCUS       DAZPLM010000154         9621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPLM010000154 DAZPLM010000000
VERSION     DAZPLM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.154"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHVC7_003153"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHVC7_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHVC7_003154"
     CDS             2192..3328
                     /locus_tag="ACHVC7_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR2117133.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHVC7_003155"
     CDS             3325..5325
                     /locus_tag="ACHVC7_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR2117134.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="ACHVC7_003156"
     CDS             5450..5911
                     /locus_tag="ACHVC7_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR2117135.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="ACHVC7_003157"
     CDS             complement(5952..6422)
                     /locus_tag="ACHVC7_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR2117136.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHVC7_003158"
                     /gene_synonym="yehT"
     CDS             complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHVC7_003158"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR2117137.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHVC7_003159"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHVC7_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR2117138.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="ACHVC7_003160"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="ACHVC7_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9385..>9621
                     /locus_tag="ACHVC7_003161"
     CDS             9385..>9621
                     /locus_tag="ACHVC7_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR2117139.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2354 a         2532 c         2468 g         2267 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgacacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg ctgacgagtg g
//
LOCUS       DAZPLM010000155         9584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPLM010000155 DAZPLM010000000
VERSION     DAZPLM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.155"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHVC7_003162"
     CDS             complement(<1..163)
                     /locus_tag="ACHVC7_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR2117140.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHVC7_003163"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHVC7_003163"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR2117141.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHVC7_003164"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHVC7_003164"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR2117142.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHVC7_003165"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHVC7_003165"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR2117143.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHVC7_003166"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHVC7_003166"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR2117144.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHVC7_003167"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHVC7_003167"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR2117145.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHVC7_003168"
     CDS             5058..6191
                     /locus_tag="ACHVC7_003168"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR2117146.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHVC7_003169"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHVC7_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR2117147.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHVC7_003170"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHVC7_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR2117148.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHVC7_003171"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHVC7_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR2117149.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHVC7_003172"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHVC7_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR2117150.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHVC7_003173"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHVC7_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR2117151.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHVC7_003174"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHVC7_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR2117152.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2430 a         2460 c         2461 g         2233 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atat
//
LOCUS       DAZPLM010000156         9500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPLM010000156 DAZPLM010000000
VERSION     DAZPLM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.156"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHVC7_003175"
     CDS             <1..108
                     /locus_tag="ACHVC7_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2117153.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHVC7_003176"
     CDS             complement(149..1231)
                     /locus_tag="ACHVC7_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR2117154.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHVC7_003177"
     CDS             complement(1228..3060)
                     /locus_tag="ACHVC7_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR2117155.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHVC7_003178"
     CDS             complement(3275..3631)
                     /locus_tag="ACHVC7_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117156.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHVC7_003179"
     CDS             complement(3689..4111)
                     /locus_tag="ACHVC7_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2117157.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHVC7_003180"
     CDS             complement(4127..4852)
                     /locus_tag="ACHVC7_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR2117158.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHVC7_003181"
     CDS             complement(4874..5620)
                     /locus_tag="ACHVC7_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2117159.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHVC7_003182"
     CDS             complement(5627..6691)
                     /locus_tag="ACHVC7_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR2117160.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHVC7_003183"
     CDS             complement(6763..7188)
                     /locus_tag="ACHVC7_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR2117161.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHVC7_003184"
     CDS             complement(7185..7448)
                     /locus_tag="ACHVC7_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2117162.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHVC7_003185"
     CDS             7560..8060
                     /locus_tag="ACHVC7_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2117163.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHVC7_003186"
     CDS             8080..8274
                     /locus_tag="ACHVC7_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR2117164.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHVC7_003187"
     CDS             8274..8564
                     /locus_tag="ACHVC7_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2117165.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8843..8995
                     /locus_tag="ACHVC7_003188"
     CDS             8843..8995
                     /locus_tag="ACHVC7_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2117166.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9007..9372
                     /locus_tag="ACHVC7_003189"
     CDS             9007..9372
                     /locus_tag="ACHVC7_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117167.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2442 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaata atgatgctat tgtttcaggc gtcaacccac cccgccccac agaatgcagg
     8701 gcaatacttc gagttaccag acagtggtca ggggttaagt agccagcccg aggcgtaaga
     8761 acatgacggc agggttcaat ctttaactat gcagcaggtt ttttgttccg ccaccccggc
     8821 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacaacg aatctctggt
     8881 gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc atgacgtata ccaaaagcaa
     8941 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     9001 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     9061 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     9121 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     9181 gagaccaaga ccgtaagttc accaatattc tcacttgagc caagcgtatt atcaggtgaa
     9241 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat tcgccgtcgc
     9301 ggtttggatg gaagttatga tcatgacgtt ctgcaagttc tcaggacaat ggtgacttca
     9361 ctggggattt gatccctgcg tcctctaagc ttttgatata gcggtcctcc aagaagcggt
     9421 aaattttgtc gaaatcatct tttccctctg gtttattcag aggattacag gacccgtaag
     9481 tagcggcata catttcaacc
//
LOCUS       DAZPLM010000157         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPLM010000157 DAZPLM010000000
VERSION     DAZPLM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.157"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHVC7_003190"
     CDS             122..952
                     /locus_tag="ACHVC7_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR2117168.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHVC7_003191"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHVC7_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR2117169.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHVC7_003192"
     CDS             complement(2081..3541)
                     /locus_tag="ACHVC7_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR2117170.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHVC7_003193"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHVC7_003193"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR2117171.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHVC7_003194"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHVC7_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR2117172.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHVC7_003195"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHVC7_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR2117173.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHVC7_003196"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHVC7_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR2117174.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHVC7_003197"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHVC7_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR2117175.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHVC7_003198"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHVC7_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR2117176.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZPLM010000158         9402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPLM010000158 DAZPLM010000000
VERSION     DAZPLM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.158"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHVC7_003199"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHVC7_003199"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR2117177.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHVC7_003200"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHVC7_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR2117178.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHVC7_003201"
     CDS             1394..1915
                     /locus_tag="ACHVC7_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2117179.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHVC7_003202"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHVC7_003202"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR2117180.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHVC7_003203"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHVC7_003203"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR2117181.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHVC7_003204"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHVC7_003204"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR2117182.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHVC7_003205"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHVC7_003205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR2117183.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHVC7_003206"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHVC7_003206"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR2117184.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHVC7_003207"
     CDS             complement(6557..7450)
                     /locus_tag="ACHVC7_003207"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR2117185.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHVC7_003208"
     CDS             complement(7447..8100)
                     /locus_tag="ACHVC7_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR2117186.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHVC7_003209"
     CDS             complement(8111..9370)
                     /locus_tag="ACHVC7_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR2117187.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2493 g         2151 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgctg
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZPLM010000159         9250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPLM010000159 DAZPLM010000000
VERSION     DAZPLM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.159"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHVC7_003210"
     CDS             complement(<1..906)
                     /locus_tag="ACHVC7_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR2117188.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHVC7_003211"
     CDS             complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHVC7_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR2117189.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHVC7_003212"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHVC7_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR2117190.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHVC7_003213"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHVC7_003213"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR2117191.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHVC7_003214"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHVC7_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR2117192.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHVC7_003215"
                     /pseudo
     CDS             complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHVC7_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(6288..6392)
                     /locus_tag="ACHVC7_003216"
                     /pseudo
     CDS             complement(6288..6392)
                     /locus_tag="ACHVC7_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6387..6629
                     /locus_tag="ACHVC7_003217"
                     /pseudo
     CDS             6387..6629
                     /locus_tag="ACHVC7_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="ACHVC7_003218"
     CDS             complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="ACHVC7_003218"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR2117193.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="ACHVC7_003219"
     CDS             complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="ACHVC7_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR2117194.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
BASE COUNT         2418 a         2427 c         2114 g         2291 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga cagatttcgt
     6541 tgttggctaa agaaaagccg gtagaaggca gtccactgtt tttactacaa atgtgcatgg
     6601 aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg aacgccatcc
     6661 tgcagtagca ttactcttta ttaaggaatt ttactgcctt atcagggaaa tcagtaaaca
     6721 gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca tcagtagtgt
     6781 attcaggcag tttatccgac cgcacggtat aaggatgcac taccagttta ttctgctgag
     6841 catcttgcac catgccagtg agtttgatat tacccggctg cgatgtttcc tcaatcaaca
     6901 tatggtaatc cggcccgata ccgtcggcat attccgcgac ttgcttcatg gcacctggct
     6961 taaacatcca gtcatagctg taattaaccc agcttccgtc cggctgtttc tgctgcgttt
     7021 cattccagtc ggtataggca atcagctgta ccagattgag atccatgccc attttaggtt
     7081 ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa taaactttat
     7141 cgtccttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc gcaatatcct
     7201 tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg atatttttcc
     7261 cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg tgcccccgga
     7321 agtcggactt acccattggg aaacgccccg gataagtctg tactttttta ccgttttcaa
     7381 tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa tctatcgcgt
     7441 agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca gtaacacgat
     7501 cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc aaatcctgtt
     7561 ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta tgctccggca
     7621 aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc gccgccattg
     7681 cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt ttcagcttca
     7741 atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg acgtttttcg
     7801 ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat cattaccata
     7861 aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct cgccgccacc
     7921 gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc cgcttttttc
     7981 ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat caggaagccg
     8041 ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat ccagtaaacg
     8101 atagtcgcga tggtcaccag tgtcataaag aaaacgccgg ttgccccacg gttgccacgg
     8161 aagactttat ccgacatcca gccgcacagc agagtgcccg gaatacctgc atattcataa
     8221 aggaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt cagataagtc
     8281 ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc gatggcgata
     8341 taccacagca gtttgttcgg cagtacgtac tgcatgaaga tttgcttcgc cgtcagctcc
     8401 tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc gatcggcggc
     8461 aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc caccagaatg
     8521 gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc catccccagc
     8581 aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca cactgacaca
     8641 atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca cggcggccac
     8701 cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc aatgctcgac
     8761 gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat caaacctgcg
     8821 ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa tttcgaaaat
     8881 ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga gaatccctgc
     8941 tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta agccgcatag
     9001 ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg atcgatctcc
     9061 gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat tgatagcctc
     9121 cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac ctgccgtttc
     9181 ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc cgcacacatt
     9241 attaaataag
//
LOCUS       DAZPLM010000160         9212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPLM010000160 DAZPLM010000000
VERSION     DAZPLM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.160"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHVC7_003220"
     CDS             73..732
                     /locus_tag="ACHVC7_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR2117195.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHVC7_003221"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHVC7_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR2117196.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHVC7_003222"
     CDS             complement(1860..2570)
                     /locus_tag="ACHVC7_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR2117197.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHVC7_003223"
     CDS             3004..3294
                     /locus_tag="ACHVC7_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR2117198.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHVC7_003224"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHVC7_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR2117199.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHVC7_003225"
     CDS             3927..3998
                     /locus_tag="ACHVC7_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117200.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHVC7_003226"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHVC7_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR2117201.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHVC7_003227"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHVC7_003227"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR2117202.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHVC7_003228"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHVC7_003228"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR2117203.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="ACHVC7_003229"
     CDS             complement(7538..7837)
                     /locus_tag="ACHVC7_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR2117204.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="ACHVC7_003230"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="ACHVC7_003230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR2117205.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="ACHVC7_003231"
     CDS             complement(9049..>9212)
                     /locus_tag="ACHVC7_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR2117206.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAZPLM010000161         9073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPLM010000161 DAZPLM010000000
VERSION     DAZPLM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.161"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHVC7_003232"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHVC7_003232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR2117207.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHVC7_003233"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHVC7_003233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR2117208.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHVC7_003234"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHVC7_003234"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR2117209.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHVC7_003235"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHVC7_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR2117210.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHVC7_003236"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHVC7_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR2117211.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHVC7_003237"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHVC7_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR2117212.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHVC7_003238"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHVC7_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR2117213.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHVC7_003239"
     CDS             8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHVC7_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR2117214.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8344..9041
                     /locus_tag="ACHVC7_003240"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="ACHVC7_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2510 c         2585 g         1990 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaa
//
LOCUS       DAZPLM010000162         9030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPLM010000162 DAZPLM010000000
VERSION     DAZPLM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.162"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            174..602
                     /gene="uspC"
                     /locus_tag="ACHVC7_003241"
     CDS             174..602
                     /gene="uspC"
                     /locus_tag="ACHVC7_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR2117215.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(609..2033)
                     /gene="otsA"
                     /locus_tag="ACHVC7_003242"
     CDS             complement(609..2033)
                     /gene="otsA"
                     /locus_tag="ACHVC7_003242"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR2117216.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2008..2808)
                     /gene="otsB"
                     /locus_tag="ACHVC7_003243"
     CDS             complement(2008..2808)
                     /gene="otsB"
                     /locus_tag="ACHVC7_003243"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR2117217.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2975..3961)
                     /gene="araH"
                     /locus_tag="ACHVC7_003244"
     CDS             complement(2975..3961)
                     /gene="araH"
                     /locus_tag="ACHVC7_003244"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR2117218.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITILATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3976..5490)
                     /gene="araG"
                     /locus_tag="ACHVC7_003245"
     CDS             complement(3976..5490)
                     /gene="araG"
                     /locus_tag="ACHVC7_003245"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR2117219.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5560..6549)
                     /gene="araF"
                     /locus_tag="ACHVC7_003246"
     CDS             complement(5560..6549)
                     /gene="araF"
                     /locus_tag="ACHVC7_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR2117220.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7346..7849
                     /locus_tag="ACHVC7_003247"
     CDS             7346..7849
                     /locus_tag="ACHVC7_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR2117221.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7928..8179)
                     /gene="yecJ"
                     /locus_tag="ACHVC7_003248"
     CDS             complement(7928..8179)
                     /gene="yecJ"
                     /locus_tag="ACHVC7_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR2117222.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8294..8380)
                     /gene="azuC"
                     /locus_tag="ACHVC7_003249"
     CDS             complement(8294..8380)
                     /gene="azuC"
                     /locus_tag="ACHVC7_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR2117223.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8643..8966
                     /locus_tag="ACHVC7_003250"
     CDS             8643..8966
                     /locus_tag="ACHVC7_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR2117224.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2334 a         2278 c         2057 g         2361 t
ORIGIN      
        1 tattttctta taattacatt tgaaattata tgatcaccgg gagtgattga ttaattgtct
       61 ttactaataa tcagactaat atttacctgt ttgaccgagt tgggattgcg tcgtttctcc
      121 attaggagta aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct
      181 atagcaatat tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag
      241 cggtatctat cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc
      301 cggaaatgta caatcaatta gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag
      361 aagaaacgca aagctttctt gataagttaa ttcaggatgc ggggtatccc gttgacaaga
      421 cgtttattgc ctacggtgag ttaagcgaac atattctgga agtatgtcac aaacatcatt
      481 tcgatttggt gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg
      541 cgaaaagagt gattgcctca agtgaggtcg atgtgctgtt agttccactt acgggagatt
      601 aaccgctcct acgcaagctt tggaaaggta gcaactttat cgcgctgctg gctttccgcg
      661 cttcgcggaa ctatctgctt taggtcgcta atgaagcact cctgccagtg gttaatatcg
      721 tttttcacga taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata
      781 gtcaatgcac gatccagcgc agctgcaact tcgtcacgat cgtaggggtt aacaattaac
      841 gccgacgtta actcgtttgc cgctcccgca aattgcgaaa gaacaagaac gcccgggttg
      901 gctgggtcct gagcagcaac atactctttt gctaccaggt tcatcccgtc acgcagtggc
      961 gtcactaagc ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc
     1021 tgattcaaat aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca
     1081 gcttcatttt cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt
     1141 ggtgcaatct gggtataacg aattttacca tgatgctgcg gatatttttc cagcaacgct
     1201 tcataggcga gaaaacgctc tggcaaacct ttggaataat ccagccgttc gacagaaaag
     1261 atattttgta cgtttttcag ttccgcttta agttgcgcca gttttggcgg cagtggcccg
     1321 gcagcctgtt tggctatttc tttcggttca atgccgatcg ggtagacttc tgttcgaaat
     1381 gctttgcccc aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa
     1441 agacaatcca ggaacgccag acgatcgttt tctgtctgga aacccagcaa atcataatca
     1501 caaagctgtt caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg
     1561 aaaggaatat gcagaaagaa accaatgcga ttattcactc cccgtttgcg taattcatgc
     1621 gcaaatggca acaggtgata atcgtggatc cagataatgt catcgtcttg caacagcggc
     1681 agtaatttat ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc aggacgctga
     1741 aattgcacca gatcgagccg ataatgaaaa gcgggccaga gaacggcatt ggagaattgg
     1801 ttgtagtatt cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt aatgttacct
     1861 tttttcacct tttttagcgg ctgatcctca ttccctgttt caccactcca gccaaaccac
     1921 agtccgcctg cggctttcag tgcccccagt atgccaacgg caaggccacc ggcactggcg
     1981 gcgtgctcgt ctggtggtgc aatccggtta gatactacga ctaaacgact catagtcatc
     2041 actcctgtta ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa gccagctcca
     2101 gacatccggc acacccgcca gtcgccatga tgcctgagtt gcacctgtgc caatttttac
     2161 tgacattccg cccagtcggt taacgactgc gaagccagat tcatcggtta aatcatcgcc
     2221 cagaaatacg ggcgttcgcc cgataaaggg agcttcctgc ataaaagctg caattgcctc
     2281 acctttactg gtacctctcg gtttgatctc gacaacacac tttccctgct gtaacgccat
     2341 ttgtggccag atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg
     2401 agcctgacga taatgcagcg caaaagccat ccctttcgcc tccagctccg cgccgggata
     2461 ctgagcgatg actgtatgca gttgcacgct aatatcacgc gcaatcgcat ccggcagatg
     2521 aacgatatgt gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg
     2581 gaagcgataa ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc
     2641 caatgcacca tcacttgcgg ttgccagtag ctgtagtcct tgcagaatat tgtcaggcac
     2701 gacgacctga tcgggatgcg gtttgatttc cgccagcgtt ccatcaagat caaaaaacca
     2761 ggcatatttc gcggatagtt caggggtttc ggttaacggt tctgtcaccc ggctctcctc
     2821 cttctctttt cttattgttt tgcgcagaac gcgctttaca gacatgtaag tatagacagt
     2881 gtgacggggg tcgccatttg caatgaagaa cagccaaccg ttgtggcggc tggctgtggt
     2941 gggaaaaaac gttaaattgt tgtggaaaaa agcatcagac agtgcgtttc gctttttgct
     3001 tgtaacggtc gaagatcacc gctgccagca ggattaagcc gcgaaccacg tactgcgcga
     3061 aaggagaaat attaagcagg ttcatggcgt tttccacggt gcctaaaatt aagatacccg
     3121 ccaccacata tgagattttt ccgatgccac ctttcagaga aacgccacct aaaacgcagg
     3181 cggagataac aataagctca taaccaatcg acgtcattgg ctgcccacta gtcatacgtg
     3241 aagccagaat aattccggct atcgcagata ccaggccaga gagaacaaag ataataattt
     3301 tggtgcgaac aaccggtaca cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca
     3361 gggtgttacg accaaaggtg gttttattca gcaacaaacc aaagataatc agacacgcga
     3421 cggtgagcca gattggcgca ggcagaccga accagttagc gtaaccaagg gcaaagaagc
     3481 tttcatcttc gataccgacc gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa
     3541 tctgcatcgt tgccagtatt gtgatcagag catttatttt cagtttggcg ataacaaagc
     3601 cattgaccag gccacagaga atgccaagca gcaaccccgc tgccacgcca atccacaggc
     3661 tttcagtcag gttgataacc accgccgtgg tgacacccgc acaggcaatt acggaggcga
     3721 cagaaaggtc aaagtcaccg gaagcaaggc agaacaacat gccacacgcc accatccccg
     3781 acatggaaat tgccaggccc aaccctttca tattaatgaa ggtggcaaaa tttgggacaa
     3841 aaatggcaca ggcgataaag agcaccgcaa acaccaccag catgccgtac tgatcccaaa
     3901 tacgcccgaa gctgaatgac gacttaggtg cgccagaacc cgatgtagaa acagaagaca
     3961 tcatactctc cttactcagg caactgcctg gctgacttta ggcatcgcaa ggctcagtgc
     4021 ctgacgctca tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac
     4081 cacaatccgg tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc
     4141 cacaccctgc gccgccagcg cataaattac gttatatatt tcgtgcttag caccaacatc
     4201 aatgctgcgc gtaggttcat ccagcaaaat gaccttcatc tcttccgata accagcggcc
     4261 cagaatggct ttttgctgat ttccgcctga gagattcatg atcagttgct cagcgcccgg
     4321 cgttttgatg ttgagcgaac gaatgtgctg atcggcattg ttttcttccc aaccgttgtt
     4381 gattacacaa ccgccgagca catgtttgcg tctggcactg atgttgatat tgtcgcgaac
     4441 ggagtgcacg ggaataatgc cttccgcttt gcgatcttcc gggcagagca tcatgcctgc
     4501 ggcaatggcg tggctcggtt tacgaatatc gatcggctgt tggtcgatat aaacctgacc
     4561 ggcggtgatt tgcgtcccgc caaacaagcc tttcattaat tcgctacgcc ccgcccctac
     4621 cagaccaaac agcccaacaa tttcaccact gcgaaccgcc agacttattg gcgtacgcac
     4681 gcctggtgct ttcacagcat caagacgcag gcgctcctcg ccataactac gaggttgcca
     4741 gccgtagata tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac
     4801 ctgctgcata tcggtaaagg ttttgacata acgtccatct ttaaagacag tgatggcatc
     4861 gctgagggca aatatttctt ccatacggtg agaaacgtat aagatcaccc gcccctcttt
     4921 tcgcagttca cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt
     4981 tggctcatca aaggcgataa ttttagcgtt acgcgccagc gccttggcga tttcaaccat
     5041 ctgccactga ccaatggaga gatatttcag cggcgtgtcc gggtcaatat ccataccaag
     5101 atgtttaagt tgtaaacccg cctcataatt cagcaatgag cgattcacaa tgccgccttt
     5161 atgcggcagc tggccgagat agatgttttc cgcgacggtc atttccggca cgagatgcag
     5221 ttcctggtaa ataatcgcca cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat
     5281 ttcctgccca ttaatcacta cagaacccgt ggttggcgca tagttgccgc tgaggatttt
     5341 taagagagtt gattttcctg cgccattttc acccatcaac gcatgaacct gaccggcata
     5401 gcagtcaaaa ctaatatccg tcagcgcctt aacgccggga aacgttttac cgatgccgcg
     5461 aaatgagaga tacggggtag actgttgcat aacgtctccg tgaatcactg gtcgttcaca
     5521 ccccctctgc atcatgcaga ggggaatttt tccggcaaat tacttaccgc ctaaaccttt
     5581 tttctccagt tcttctttaa agttgtcacg cgtgatcagc accacgtcgg taacttcggt
     5641 aaattttggc ggttcaacgt cttttgctac ccagttgtaa agcatttcgc tggatttata
     5701 gccatgtacg tccgggcttg gcagcaggga accgtagaag ccggttgcct gtgctttaga
     5761 cagttcgctc aacgcatcca caccgttaat gccaatgccg atgatatcgg cagctttaaa
     5821 gccctgacct tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca taccgacgat
     5881 cagccaatgt ttaacttccg gatgttgaac cagcattgag ttggcagcgt caaatgcccc
     5941 cgggatgtcg ttagatttgg taggtacctg ataaatttgt ttttccggga atccggccgc
     6001 tttcagcgca tccatagaac ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt
     6061 aatcgccatc accgcgcttt ctttgacatc ccagccacgt ttctgcatct ctttatacag
     6121 ttcctgaccc tgacgttcgc caattttagt cgccgccatc atcaccagcg gaacggtatc
     6181 cattggctta cctttggcgt taacaaactg gtcatccacg gcaatgactt tcatatcgta
     6241 gccacgcgct ttcgcgacga tggcagagcc gagtttgggg tccggagtac aaataacgaa
     6301 accttttgcg ccactggcag ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg
     6361 cacggcaatc ttaataacct caaaccctaa atccttcccg gctttatcgg caaacttcca
     6421 ttcggtctgg aaccacggct cttccggttg cttcaccaga aaaccgagct tcaggttctc
     6481 cgccatagcg gattgtgaca taacggctgc cagaccaatg gctgccaggg ctttagtaaa
     6541 tttgtgcatg gttctctcca gctttagtgt cgttttgtgt agggcaaaaa cgaatgactt
     6601 tcgttaaatt aatcggaaaa caaagcatta ccttttatct aaaagataag tgaccgtgtt
     6661 gacatagttt tagcgagaaa ttaattctcc ataggagagc aatatcacat cgcagaatta
     6721 cagtgagaac gtgcataaat ttagcgggaa aagacataag ggaaagccaa tttgtcagac
     6781 aaattgtcga atgcacagca gattaatcca taagattagc ctggaaatcc ttgttgtctt
     6841 tggtacccat gcgggatgtc ttctttttaa ccagtcaata ggccgcatta cctggcgttg
     6901 agtttttgaa atggtgtaat aaccgcaact caaagatgtg gaaaatgcac gtcattcatt
     6961 tcgccattaa ttatcactgt gctcattaat taacagaaca cgtataatga gagccatctc
     7021 gcaaaaatga aaaaacgttt tataaaatca tcacttcatc atgaattcaa attcattgat
     7081 taatatcaac aagatacaaa aagcactatc attaaaattc attgcagtta cattgatttc
     7141 atcaatgaaa tgtaaaaata tataaacttg atgatttaag cattttctta tacccgttca
     7201 gacgttattc ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg
     7261 cagaaaggaa ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc
     7321 acttctggat ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc
     7381 tcaaatgaac cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc
     7441 tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac
     7501 ccaaatgatg cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc
     7561 cattgatgtt cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga
     7621 agaatacgag caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa
     7681 tgatgattca accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggtct
     7741 gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt
     7801 gcagaccgac caacatgttc tgaatgtcgt gtcacaccag ctgcattgat catcatcggc
     7861 gctaatgcat tgcgccgatg aaggttttga gaaaccgctg cctcatctgt ttgaagcagc
     7921 ggttttttta atgggattca ccctgtgggg taaacttgag ttcaataagc gcgatggctt
     7981 tttggattgc ccgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat
     8041 tcttcagctg actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat
     8101 ccagaatttg tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg
     8161 cattcagcgg ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg
     8221 gcttcacatc ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg
     8281 agcaggtttt ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat
     8341 agttattgaa catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg
     8401 ttatacaggt gttattgtct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt
     8461 tatgtgatac aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca
     8521 gcccccctat ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc
     8581 ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga
     8641 ccatgagact tctgattctg actctttcat taataacgct ggcggggtgt acggttactc
     8701 gccaagccca tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc
     8761 aggctttttt gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg
     8821 catgccagca ggaaggctat acccacgcgg tcccctttgg tcagccagta ggcaactgta
     8881 gcctttttgc gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc
     8941 attccgcctt ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta
     9001 aagaaatagc ggtaatgcgt tttattccca
//
LOCUS       DAZPLM010000163         8909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPLM010000163 DAZPLM010000000
VERSION     DAZPLM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.163"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1584
                     /locus_tag="ACHVC7_003251"
     CDS             <1..1584
                     /locus_tag="ACHVC7_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR2117225.1"
                     /translation="FKKINANELAAERDAQTATEYAKKIRNNPVIQDSTKQWINTTPI
                     IEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIPVRISADVWTYLNGTGTGSTQ
                     QMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELNISGLPALLQTVSSRLGISWK
                     YDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSSSGSTGSTSSGGMTGDASNTQ
                     TTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTVTDTPDVLDSVQEIVNKRNNE
                     MSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLSLGGSFGDAAENVITSGVSIV
                     DGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPMQIANQQGFIESVTTDSTANV
                     GSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSLSDKPTFEVFESGGSKAQTPN
                     VDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITPSFFGLGGGVNSEDGDTILVV
                     LITPNVLNSGSSAVIKNVN"
     gene            1623..2945
                     /gene="pilO2"
                     /locus_tag="ACHVC7_003252"
     CDS             1623..2945
                     /gene="pilO2"
                     /locus_tag="ACHVC7_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR2117226.1"
                     /translation="MEVTLSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTF
                     ARKNDAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAI
                     CELGDLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPDPGWKLYQPESWDISQ
                     ALPSLTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKE
                     AAAEAARIRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLA
                     ECSTSGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSF
                     DVSPDPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYE
                     LMIQTSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            2929..3423
                     /gene="pilP"
                     /locus_tag="ACHVC7_003253"
     CDS             2929..3423
                     /gene="pilP"
                     /locus_tag="ACHVC7_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR2117227.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            3448..4986
                     /locus_tag="ACHVC7_003254"
     CDS             3448..4986
                     /locus_tag="ACHVC7_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR2117228.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVQVYIADNSKGQRAVQ
                     AYIGTLNVKYPGQVNITSTTLDVIASRYQDSEKRSDSNSSQHLNSNQEKVISYLAKAN
                     KLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            4977..6086
                     /locus_tag="ACHVC7_003255"
     CDS             4977..6086
                     /locus_tag="ACHVC7_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR2117229.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            6131..6688
                     /locus_tag="ACHVC7_003256"
     CDS             6131..6688
                     /locus_tag="ACHVC7_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR2117230.1"
                     /translation="MSSINILNMRSVFSSLSAHRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEASVPKKNCMAMVNALRSSSAISKINN
                     TSTSTVDAATVCSSDSNTLTFSTDS"
     gene            6755..7237
                     /locus_tag="ACHVC7_003257"
     CDS             6755..7237
                     /locus_tag="ACHVC7_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR2117231.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            7241..7876
                     /locus_tag="ACHVC7_003258"
     CDS             7241..7876
                     /locus_tag="ACHVC7_003258"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR2117232.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            7889..>8909
                     /gene="pilV"
                     /locus_tag="ACHVC7_003259"
     CDS             7889..>8909
                     /gene="pilV"
                     /locus_tag="ACHVC7_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR2117233.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGAIL"
BASE COUNT         2639 a         2005 c         1929 g         2336 t
ORIGIN      
        1 ttcaaaaaaa tcaatgctaa tgaacttgct gccgaaagag acgcacaaac agcgactgaa
       61 tacgcgaaaa aaattcgtaa taacccggtt atccaggatt caacaaaaca atggattaat
      121 acaacgccaa ttattgaaaa gcaggtaaag caatcagcgc cgccctgccc gattgtgatt
      181 aatactcgtt cagatattga gctacgccag atcgcacaac gcatcacaca aacctgtcat
      241 atcccggtac gaattagcgc cgatgtctgg acctatctga acggtacagg cactggctca
      301 acacaacaaa tgacaggctc aattccagca cctgatgcaa atggaatggt tcctctggcg
      361 tcagtaggca gtcagccagt aaaagtctct tctggcggta catcactccc tgaactgaat
      421 atctccgggc ttcctgccct actgcaaacc gtatcttcac ggctgggaat atcctggaaa
      481 tacgataaag gtcgtatcac attcttctat ctggaaacgc gctcattccc gattacatat
      541 atggacagca atgtggcata caactcaaaa gttgtgagcg gcacaatgtc ttccagtggc
      601 agtaccggct ctacaagttc tggcggtatg actggcgacg catcaaatac gcaaactaca
      661 accgtagaaa tgaaatcatc gctgtacaac gacctgaaaa gcgaagtttc ctcaatgctg
      721 acaccaggta cagggcgcat gtatctgtcc acaggttcac tgacagtaac agataccccg
      781 gatgtactgg acagcgttca ggagattgtg aacaagcgca ataatgaaat gagtcgtcag
      841 gttgtgctga acgttgaaat cctgagtatc aagaaatcaa gccaggaaca ggcgggaatc
      901 gactggaacg cagtatttaa tgatggtcat cttggtctga gtcttggtgg ctcatttggt
      961 gatgccgccg agaatgttat taccagtggt gtatccatcg tggacggtaa gctggctggc
     1021 tcaaaagcat ttctgaaagc cctgtcgtca caaggcagcg tcagtgtcgt aacgcaaaac
     1081 tccgctgtga ctaaaaacct gacgcctgtc cctatgcaaa ttgccaatca gcagggcttc
     1141 attgaaagtg tcacaaccga ttctaccgcc aatgtgggat catccacgtc actgaatgcg
     1201 gcaacaataa cgaccggatt caatatgact ctgcttccat acattcagcc ggattcacaa
     1261 aacctgcaat tgctgttctc aatgagtctg tctgacaaac ctacttttga ggtgttcgaa
     1321 agtggcggca gtaaagcaca aacaccgaat gtggatttga aaacaattaa tcagactatt
     1381 gacctgaaat caggccagac agtcattctg agtggattcc agcaatcaaa ccggaaaacc
     1441 agtaagcaag gtgtaataac cccctccttc tttggacttg ggggtggcgt taacagcgaa
     1501 gacggcgaca cgattctggt cgttttgatt acaccaaatg tgcttaattc cggctccagt
     1561 gcagtaataa aaaatgtgaa ctaattaact gaaaactccc tgaaaaaaca cagggagttt
     1621 ttttggaggt cactttgtct gagaataatc agaataaccg gaatttcact tcggttatta
     1681 aaaacaagcg tgcttttttc tccggtctgg actggaaaac gcttccttca gaagaaaaga
     1741 atgcgcgtac attcgcaaga aaaaacgacg ctgaatattt tctctcctgt cagtatcagg
     1801 attcagaaaa tgaaaccaaa acgatggtgg catttatcag aaaagaggat cttcccacag
     1861 gggcatcctc attctggtca ctggcactga tgataaaacc cctcatcgaa cctgacggat
     1921 acgctatctg cgaactcggt gatctttatg gctttgtttc ctgcgtcaat aacgttctcg
     1981 tcaacgatgt tgtaggtaac aaaagtcaga tcatgagtgc tctgacaaca tttcttgagt
     2041 ttaatgaaac accagacccc ggctggaaac tataccagcc tgaatcatgg gatatcagcc
     2101 aggcactccc ctcactcact ctgtctgcgt tgattgacgt aaaaaaaccg ccgaaagagg
     2161 cggcgtttac tcgcgtatcc cgaaaacgtc agttcatgat ttatggcgga agtgccattc
     2221 tggccatact gctctggaac gggatcacca tgtaccagga ataccgggaa aaagaagctg
     2281 cggcagaagc tgcacgtatt cgtctggcaa aagaaatggc agacaaacag gcaatacaga
     2341 ttgccccacc gtggcaacat ttacctgaaa taaaaccatt tattgataaa tgcattgata
     2401 aatgggatgc actgccctta tcgatcgccg gatggcgttt tgaccttgct gaatgcagca
     2461 catccggtaa tgacgggctg ttacgcacat catataagga actgagcggc gttacggtcg
     2521 aggacttcag cacacggata cgagaaatat tccaggggac aaccacagca acttttgtat
     2581 tgccagaagg cagcgccggg ggattttctc ttcctgtcag ttttgacgtt tcgcctgatc
     2641 caattacccc ggacacattg ccacaggcaa cagatattca ggaacgcctg acaacatttg
     2701 cacaaaaaat gaggttaaaa ctcacctggc aggaaattga aaacacaaaa acagatgaag
     2761 aaggccgtcc aataattctc ccgtggaacg agtatgaatt aatgattcag accagcaccc
     2821 cgccttcaat acttttcgct aactttcatg aacctgccgt tcgcttccag tatgcaggaa
     2881 taaaactgga agaaggaaga ctgaattatg agattaaagg tgcattctat gttaaaaata
     2941 attaaaccaa cgattatttc tgtctgtttt ttcagttcac ttgcttacgc aacagaatct
     3001 gaaagcacac caaaagacga aataaaatat tcatctccga ccattttaca ggtagagcag
     3061 attcaggctg aaacggtaat ttacgaagca aagctggcgc gtcagaaggc tgtcagtgaa
     3121 ttgcgtaatc ttgatttgaa tggtgtagcg tccgcaacgc cagcaataac gccctcttcc
     3181 gggtattctt ctcttgcaga taccgatata ccagcgcaac gtgtagccgc taaatcactt
     3241 cgtgttattg aaatatttgg tacaccacaa caaatgtcag cacgaatttc tctcctggac
     3301 gggtcagtaa ctgatgtcaa ggtcgggcaa agtattcctg gtacttctta taaggtggca
     3361 tcaatcactg gtaatggcgt ctggattcag aatggtacag agaaacgcca acttattctt
     3421 taatgctatt gaaagagacg acacataatg tttttttctg acgcagacta tcccggaaaa
     3481 gatatttctg atgttgtata cgtcagtata tctgaaaaag acgataaacc tgttcaggtt
     3541 tatatcgctg ataacagtaa aggacagcgt gctgttcagg cttatattgg cactctgaat
     3601 gttaaatatc ccggacaggt aaatataacc tcgacaactc ttgatgttat cgcatcccgc
     3661 taccaggaca gcgaaaaaag gtctgattca aactcatcac aacacttaaa cagcaaccag
     3721 gaaaaggtca ttagctacct ggcgaaagca aacaaactgg gaagttccga tttgcacatc
     3781 acacctggtc gggacggctc cgagttcact tatgtcgaag cacgcgtgca tggggaactg
     3841 gaaattctgg atgtcatccc gcgtaaagaa gggctggaat tgctgggtgc tgcctattct
     3901 ggaatgagtg atgttatcaa aggtacgcag tttgaccctg caataccaca ggatgcacgt
     3961 atcgcagaga attttctgaa gcctgtaaat ctttttgggg cgcgctattc gcattatccg
     4021 tgcgtgggtg gtgtgtacgc ggtcttccgc ctgattaaag atgacagcga ggatattccc
     4081 actttcgaag aacttggcta catgccgcaa caaatccaga caatcaggcg aatgttacaa
     4141 cgcccggaag gaattatcgt tttatccggc cctacagggt ccggtaagtc aacgactctg
     4201 agaacggctt cagaagccta tctgagcacg tttggtttca accataacga taatatgcgt
     4261 ctcccccgaa aacgcctttt caccattgaa tcaccgccgg agggccgcat tcccggcgca
     4321 atacagacgg cagtcaggga ttccgtcgat ggctgggttg acgcgattaa atccgctatg
     4381 cgacttgatc cagacgcgat actgaacggc gaaattcgtg atcacgcatc ggcattagca
     4441 gcaatcaaag cctcgatgac aggccacctt ctgttgacga ccttacacgc caacgatgcc
     4501 ctgaatatta ttgagcgcct tgaaatggag aatattcagg ccaggctgat tgcagatcct
     4561 cttctgttta tcggtctgct gagtcaacgg ctggtacaaa aactctgtcc ttactgtaag
     4621 cgcacttatg ctgaaatggc agacaaactg agtacagaag aacggaaact cattgaggaa
     4681 aattgcctgc cggaacaggt ccggttgcgc aatcttgacg gttgcgagca ctgttataaa
     4741 ggcgtaacgg gaaggacggt tatagcagaa gtcgtctcac ccgacgcacg tttttttcag
     4801 ttatatcgtc agtccgggcg tctggaagca aaatcatact ggcactatga acttggagga
     4861 attacccgta atcagcactt attacacctg attaatcatg gtctggtaga tccgctgtcc
     4921 gcacacttca tatcccccat tgacgaagat aaatactcaa tgctcccgga ggggacatgg
     4981 cgataaaaga tatatttgag aaactcaatt cgatagaaat tgatttctct ggaatatccc
     5041 actggctggc aaaatacaca ttcaggtatt ccagaataag tttatatgaa gatttatctg
     5101 gaatgttaaa tgatggcata aatgtaaagc gtgctcttac acacctgatt gatgtagaaa
     5161 cagactacgg aaagaaaacg gggtcttctg caacctacta tatttgttgt gagtgcctta
     5221 atgcactgaa taacacaggg gcaatatctt cagcattaaa aaaatatata aaggaggatg
     5281 aatatcaatt aatatccagc ggtgaaattc gcggcgacct tgccggggga ttagttcagg
     5341 ccataaaaat tgtcagaagt cgttctgaaa tgacaatgac tcttgttatg tcgatgattt
     5401 atccatccgt actggttttt ggttgtctgg caaacatgta tatggtccac gataccataa
     5461 cacctgtttt cctttctctc gcaccgaaag aacgctggac aagttctatg cgattactga
     5521 cagatacgtc tggtttcctg atagaaaacg gaacgtatat tctctgcttc tgcatcgtac
     5581 tgtttttcct gatcagatat tcgcttgcac gttatacagg accgggccgt cagtatcttg
     5641 attacattcc cccctggtca ctttataaaa catttaatgg tgtaagtttt ctcttcaaca
     5701 tggcctccat gttaagtatt gatattcctg ttgcaaaagc actggaaagg ctggagaaaa
     5761 acagcactca aaacagatgg ctactcgaac gaataaaaga aatcaggaga tatgttttac
     5821 ttggacagtc acttgcaatg gcaatgaaaa gttgcggtta tgacttccca tcgaaattat
     5881 gcattaacaa actattactt cattctgaaa atgctgacaa tgcattgatg atgtctaact
     5941 atgctgataa atggctggag gaagcaaaag gaaaagtcaa atcaacagga gtctggataa
     6001 ctgtagtatc cgcattaatt gttttcgcat ttattgtaat gatgattact gctctctacg
     6061 atgtcagtaa cgtcctgcaa cactaatccc taatgttttt aacctctcaa taaataagga
     6121 attatttatt atgtcttcta ttaatatttt aaatatgcgt tctgtttttt cttctctttc
     6181 tgcacaccgt aaaaaagaac aggacaaagg ggcaacactg atggaagtgt tattggttgt
     6241 tggtgttatt gttgtacttg cagcaagtgc atataaactc tattcaatgg tgcaatccaa
     6301 cattcaatca tccaatgaac aaaacaatgt cctgacagtg attgccaaca tgaaatcact
     6361 gaaattccag gggcgttata ctgacagtaa ctatattaag actctctacg cgcaaggatt
     6421 actgccatcc gatatgatcg cagatacaac tggcgcaagc gcgaaaaacc cgtggggcgg
     6481 ctccgttaca atcacaacct cttcggataa atactccttc aatgttgttg aggccagcgt
     6541 accgaagaaa aactgcatgg caatggtaaa cgccctgcgt tcttccagcg caatctccaa
     6601 gattaacaac acttccacat caactgtcga tgcggcaact gtttgctcat ctgacagcaa
     6661 tacgctgacc ttcagcactg attcctgatt tttcatgtgc atcggataag cgccttcggg
     6721 cgcttatttt ttccatgttt acaggaggag tattgtgcgt tctgttcttt atatcctgct
     6781 atcatgtatt tcatcattaa tcttttcatt gcaacccgca tattcagcga caacgacccg
     6841 atttgatgcc tgctttgcac aagccgggca acgttatctg attgatcccc tgcttctgaa
     6901 aagcattgcc actgtagaaa gttcactcat accagaccgg ataaatcaca acaaagaaaa
     6961 aggaaaaata ttaagcaccg attacggact gatgcaaatt aattcatcac acatccccaa
     7021 cctgaaaaaa cttggcgtaa taaaagacaa atcagagctt attgataacc cctgtctcaa
     7081 tatacaaatt ggcgcgtgga ttctggcaac acattttcag aaatgtggca taaactggag
     7141 ttgtcttggc agttataacg ctggtttcaa agaaagtaac gaacagaaaa gaatcaaata
     7201 cgcacgctat gtctacaata aatacatggt aaggtaactt atgctaaaca gcatatttaa
     7261 tacggcgata ctttgctgtg caaatatcgt ctgtcaatgc tacgcctaca atgaagtaaa
     7321 attccgttta aataaactta atgtatctta taaaaccggc acagagaaat attactgtct
     7381 gatactgaca acgcttgccg ttctgtatct ttcattaaac cagctatcac tgattcaatc
     7441 cataatagtg attgttttct atgctttcct tacattaatg gcctgtatag accttctttc
     7501 atttctacta ccacgccttt ataccgtgac atttatcttc tccggcctgt tatatcagac
     7561 atggaataac aatatattgt ctggtttgtt ttgtgcaatg ctgatgttct tcattatgct
     7621 gtttgtaaga ctttactttg catataaaaa cggaacggaa agtttcggca tgggagatgt
     7681 gctacttatt gcaggaactg gcgtatggtt tccaacgcct gagattgcct gttcaattgt
     7741 atttattgcc gttatcggcg gaattatttt cttcactctt ggcggattaa acaagcaaaa
     7801 aaaatatatc ccattcggtc cgttcctttg tggcggaatg tttgtttact cactcgtacc
     7861 gggaatatta ttctgaggat taagagcaat gaaaaagaca gataaaggtg tatctctcct
     7921 tgaggtatta cttgtcatcg gcataatggt gatggttatc cctaaagttt atgaaaacat
     7981 tgaaaaccat ctgaataatg ttcgctggca gaatgcagca gaacacgcaa atacctataa
     8041 caccgctgtt cgtaattatg ttgctgacaa tgcatcaaca ctacttgctg gctctttacc
     8101 caaaacaatc acgccagcaa cactcattca gaaaggctat ctgaaatctg gtttttctga
     8161 atccaatttt ggacaaagct acattaccgg tattgcaaag aacagcaaaa catcccgtct
     8221 ggaagccctg acctgtagta atggcggtca gtccctgtca gaagccggaa tgcgctcggt
     8281 agcaagcatg attgaagggc ttggcggcta catcaacagc agcaagcagg cgattggcgc
     8341 gggtggcggc tggtctgaca cgccttcaaa ctatgggctg aactgcgcga ccgggcatat
     8401 tgcaatggca cttgttggcg ctgatttgca ggaaagcgac cgtttgtatc gttactccat
     8461 aactaatcgt cccgacctta acaggatgca tacggcgatt gatatgaaca gcaacaatct
     8521 gaacaacgtc ggcactttaa acggcaacgc cgccgcgctc tccggcgata tcagcgcacg
     8581 taacggtacg ttctctggcg caatttcagg aaatacagcc acaaccaacg gtgatatcac
     8641 ttcgaataat ggctggctgg tcacaaaaaa cagcaaaggc tggatgaaca gcacctatgg
     8701 tggcggctgg tatatgtccg attcgtcctg gttgcgcagc gtgaataata aagggattta
     8761 cactggtggc caggtaaaag gtggaactgt acgcgctgat ggacgcctct atacaggcga
     8821 gtatttacag cttgaaaaaa cagcaacggc tggcgcttcc tgctccccta acgggcttgt
     8881 aggccgcgac agtacagggg cgatacttt
//
LOCUS       DAZPLM010000164         8841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPLM010000164 DAZPLM010000000
VERSION     DAZPLM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.164"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            124..1522
                     /gene="ulaA"
                     /locus_tag="ACHVC7_003260"
                     /pseudo
     CDS             124..1522
                     /gene="ulaA"
                     /locus_tag="ACHVC7_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHVC7_003261"
     CDS             1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHVC7_003261"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR2117234.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1853..2314
                     /gene="ulaC"
                     /locus_tag="ACHVC7_003262"
     CDS             1853..2314
                     /gene="ulaC"
                     /locus_tag="ACHVC7_003262"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR2117235.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2328..2978
                     /gene="ulaD"
                     /locus_tag="ACHVC7_003263"
     CDS             2328..2978
                     /gene="ulaD"
                     /locus_tag="ACHVC7_003263"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR2117236.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2988..3842
                     /gene="ulaE"
                     /locus_tag="ACHVC7_003264"
     CDS             2988..3842
                     /gene="ulaE"
                     /locus_tag="ACHVC7_003264"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR2117237.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3842..4528
                     /gene="ulaF"
                     /locus_tag="ACHVC7_003265"
     CDS             3842..4528
                     /gene="ulaF"
                     /locus_tag="ACHVC7_003265"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR2117238.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4625..5176
                     /locus_tag="ACHVC7_003266"
     CDS             4625..5176
                     /locus_tag="ACHVC7_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR2117239.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5251..5526)
                     /gene="yjfY"
                     /locus_tag="ACHVC7_003267"
     CDS             complement(5251..5526)
                     /gene="yjfY"
                     /locus_tag="ACHVC7_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR2117240.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5853..6248
                     /gene="rpsF"
                     /locus_tag="ACHVC7_003268"
     CDS             5853..6248
                     /gene="rpsF"
                     /locus_tag="ACHVC7_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR2117241.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6255..6569
                     /gene="priB"
                     /locus_tag="ACHVC7_003269"
     CDS             6255..6569
                     /gene="priB"
                     /locus_tag="ACHVC7_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR2117242.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6574..6801
                     /gene="rpsR"
                     /locus_tag="ACHVC7_003270"
     CDS             6574..6801
                     /gene="rpsR"
                     /locus_tag="ACHVC7_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR2117243.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6843..7292
                     /gene="rplI"
                     /locus_tag="ACHVC7_003271"
     CDS             6843..7292
                     /gene="rplI"
                     /locus_tag="ACHVC7_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR2117244.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7404..8375)
                     /locus_tag="ACHVC7_003272"
     CDS             complement(7404..8375)
                     /locus_tag="ACHVC7_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR2117245.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8428..>8841)
                     /locus_tag="ACHVC7_003273"
     CDS             complement(8428..>8841)
                     /locus_tag="ACHVC7_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR2117246.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2130 a         2162 c         2350 g         2199 t
ORIGIN      
        1 tttgcgggtc gcgtcacatt taatcataaa taatcttgtt gtgattactt ttgaaaatta
       61 gagtgagtgc acaacattcc gggtgtgcgg aatacccggt tacctcttct tcaggagatc
      121 gttatggaga tcctctacaa catctttacc gtgtttttta accaggtcat gaccaatgcc
      181 ccgttgttgc tgggtattgt gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc
      241 gttattatta aaggcacgat taaaaccata attggtttca tgttgttgca ggcagggtcc
      301 ggcatcctca ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt
      361 aaacggcgca atttccgata cttacgcgtc gatgatggca accatcgacc gcatgggcga
      421 tgcctatagc tgggtaggtt acgcggtgtt gttagcgctg gcgctgaaca tctgttacgt
      481 gctgctgcgt cgcattaccg gtattcgtac catcatgctg actggtcata tcatgttcca
      541 gcaggcgggg ctgattgccg ttacgttgtt tattttcggc tactccatgt ggacaaccat
      601 tatctgcacg gcgattctgg tttcgctcta ctggggcatt acctccaaca tgatgtacaa
      661 gccgactcag gaagtgacgg acggctgcgg tttctccatc ggtcaccagc aacagtttgc
      721 atcatggact gcctataaag ttgcgccgtt cctcggcaaa aaagaggaaa gcgttgaaga
      781 cctcaaactg ccaggctggc tgaatatttt ccacgacaac atcgtctcca cggcgattgt
      841 catgaccatc ttctttggtg ccattctgct ctccttcggt atcgacaccg tgcaggcgat
      901 ggcaggcaaa gtgcactgga cggtctatat cctgcaaacc ggtttttcct tcgcggtggc
      961 gatcttcatc atcactcagg gtgtgcgcat gtttgtggcg gaactctctg aagcgtttaa
     1021 cggtatctcc cagcgcctga tccctggtgc ggttctggcg attgactgtg cggctattta
     1081 tagcttcgcg ccgaacgccg tggtctgggg ctttatgtgg ggcaccatcg gtcagctgat
     1141 tgcggttggc atcctggtcg cctgcggttc ctcgatcctg attatcccag gctttatccc
     1201 gatgttcttc tccaacgcca ccatcggcgt gttcgctaac cacttcggcg gctggcgtgc
     1261 ggcgctgaag atttgtctgg tgatggggat gatcgaaatc tttggttgcg tctgggcggt
     1321 gaaacttacc ggtatgagtg cctggatggg gatggcggac tggtcgattc tggcaccgcc
     1381 gatgatgcag ggcttcttct ccatcggtat cgcctttatg gccgtcatca ttgtaattgc
     1441 actggcttat atgttcttcg ctggccgcgc actgcgcgca gaagaagatg cagaaaaaca
     1501 actggcagaa cagtctgctt aataaggagt tttgattatg accgtacgta ttctggctgt
     1561 gtgtggcaac ggacaaggca gttccatgat catgaggatg aaagtggacc agtttttaac
     1621 ccaatcaaac attgaccaca cggtaaatag ctgcgcggtt ggcgaataca aaagcgagtt
     1681 gagcggcgcg gatatcatca tcgcttctac gcacattgcg ggcgaaatca ccgtgaccgg
     1741 caacaaatac gtggttggcg tgcgcaacat gctctctcct gccgactttg gcccgaaact
     1801 gctggaagtg atcaaagcgc atttcccgca ggatgtgaag taaggacgcg ccatgaaatt
     1861 acgtgattcg ctggcggaga ataaatccat ccgcctgcag gctgaagctg agacatggca
     1921 ggacgcagta aaaatcggcg ttgacctgct ggtggcggat gttgtcgagc cgcgttacta
     1981 ccaggcgatt ctggatgcgg ttgaacagca cggtccgtac tttgtgctcg caccaggcct
     2041 ggcaatgccg cacggtcgcc cggaagaggg cgttaagaaa accggttttg cgctggtgac
     2101 gctgaaaaag ccgctggagt tcaaccacga agataacgac ccggtggata tcctcatcac
     2161 catggcggcg gtcgatgcca acacgcacca ggaagtgggc atcatgcaga tcgtcaacct
     2221 gtttgaagat gaagaaaatt tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact
     2281 ggatctcatt gaccgcacca acgcggcagc ttaagaagga attgaagatg tcattaccga
     2341 tgttgcaagt cgcgctggac aaccagacta tggatagcgc ctacgaaacc actcgcctga
     2401 ttgccgaaga agtcgacatt atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc
     2461 gtgcggttcg cgatctgaaa gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa
     2521 ttgccgatgc gggcaaaatt ctttctcgta tgtgcttcga agccaacgct gactgggtga
     2581 ccgtgatttg ttgtgcggat atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt
     2641 ttaacggcga cgtgcagatc gaactgaccg gttactggac ctgggaacag gcgcaacagt
     2701 ggcgcgacgc aggtattcag caggtggttt atcaccgcag ccgtgacgcg caggccgctg
     2761 gcgtggcgtg gggtgaagcc gatatcactg cgatcaaacg tctttccgat atgggcttca
     2821 aagtcaccgt caccggtggc ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa
     2881 ttcatgtgtt tatcgccggt cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc
     2941 gtcagttcaa acgttccatc gccgaactgt ggggctaagg agcggatatg ttgtccaaac
     3001 aaatcccgct tggcatctat gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc
     3061 tgcgactggc aaaaacgttg ggcttcgatt ttgtcgagat gtcggtagat gaaactgacg
     3121 agcgcctgtc gcgcctcgac tggagccgcg agcagcgtct ggcgctggtg aatgcgattg
     3181 ttgaaaccgg cgtgcgtgtg ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg
     3241 gcagcgaaga tgacgcggtg cgggcgcagg ggctggagat tatgcgtaaa gccatccagt
     3301 tcgcccagga cgttggcatt cgcgtgatcc agctggcggg ctatgacgtt tactatcagg
     3361 aagccaataa cgaaacgcgt cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg
     3421 cgagccgcgc gcaggtgacg ctggcgatgg agatcatgga ttatccgttg atgaactcca
     3481 tcagcaaggc gctgggttac gcgcactatc tcaacaaccc gtggttccag ctctacccgg
     3541 atatcggcaa cctgtcggcg tgggacaacg atgtgcagat ggagttgcag gccggaatcg
     3601 ggcatatcgt cgcggtgcat gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat
     3661 ttggcgaagg tgtggtggat ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt
     3721 gcgggccgta cctgattgag atgtggagcg aaacggcgga agacccggcg gcagaagtgg
     3781 tgaaagcgcg tgattgggtg aaagcgcgca tggccaaagc aggcacggtg gaggcggcat
     3841 aatgcaaaag ctaaaacagc aggtgtttga agccaacatg gatctgccgc gctatgggct
     3901 ggtgaccttt acctggggca acgtcagcgc tatcgaccgc gaacgcgggc tggtggtgat
     3961 caagcccagc ggcgttgcct acgaaaccat gaaagcagac gatatggtgg tggtcgatat
     4021 gagcggcaag gtggtggaag gggagtatcg cccgtcttcc gacaccgcga cgcatctcga
     4081 actctaccgt cgttacccgt cgcttggcgg cattgtccat acccactcca ctcatgctac
     4141 tgcatgggcg caggcggggc tggcgatccc ggcgttaggc actacgcacg ccgactactt
     4201 ctttggcgac atcccgtgta cgcgcggatt aagcgaagaa gaggtgcagg gcgagtatga
     4261 actgaacacc ggcaaagtga ttatcgaaac gctgggtgac gccgagccgc tgcatacgcc
     4321 ggggattgtg gtgtatcagc acggtccgtt cgcctggggg aaagatgccc acgacgcggt
     4381 gcataacgcg gtggtgatgg aagaagtggc gaaaatggcg tggattgcgc gcagtattaa
     4441 cccacaactg aatcacatcg acagcttcct catgaataaa cacttcatgc gtaagcacgg
     4501 tcctaacgcc tattacgggc agaagtaagc agtgcgcccc ggaattatta cctcctgcgg
     4561 gatgtaattg gtctgattcc ggggccgctt cagttaaaaa atcgtataaa caacaggtgt
     4621 ttttatggct atcaatggat tgtttgtcag aacggatccg caacgtcgcc gctatggcgt
     4681 tgcgttactt gtgggtgtac tttcaggaat tatttcagca tttgttaaat ggggagcgga
     4741 agtccccttg ccaccgcgca cattttccgg tggtcgcgat gaatttaacc cgccgtatat
     4801 ctttctacgg gattatttag gtatcgaccc aacccaaact gtgtatacat tctcggaaca
     4861 tattatcaac ccggtgatgg tgacgcatat catcttctca ctggtttttg cgatagggta
     4921 ttgtattgtc gcggaaattt tcccgaaagt gaaattatgg caggggatat tagcagggct
     4981 tattgtgaca gtggcagtcc acggtatatt ttgcccggcg ctgaatttaa cgccgccgct
     5041 cactcaattg ccttttgacg aatatgcctc tgaaatcctg gggcatattt tctggttctg
     5101 gataatagaa attatgcgcc gcgacttacg taatcgcatt actcatgagc cagatccgga
     5161 agtcgcaatc cggtgaataa ccgcataaaa aagccccctg cgaagggggc aaagcaaact
     5221 atggcaatgt ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat
     5281 attgccgtta ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccctgcgc
     5341 atctgtctct tcatttaatg cctgacgcgc ttcgctttca gtggcgaaat tatgattgat
     5401 ataaatcacg cctaagcttt gcacatcatc catgtttctg gcctggtggt tattaatttc
     5461 aatggctgcc catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact
     5521 gaacatagat acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga
     5581 tttaatttta atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa
     5641 aaaacaccat gatgaagtgt gatgaactcc aaatcagcgg tttagaggtt aattgcgaaa
     5701 ggggagattt atttcggctc tgcccttgag tttagcgggg catacaagta ctataacgcg
     5761 tcatttttca gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac
     5821 aggaggctga ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg
     5881 tccatcctga ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca
     5941 ctggtgcaga aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc
     6001 cgatcaacaa actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag
     6061 tgatcgatga gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta
     6121 tgcgtaccaa gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc
     6181 gtgagcgtcg cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg
     6241 aagagtaatt tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc
     6301 ttcgaaaggt cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg
     6361 tgcaggagga agccggtttt caccggcagg cgtggtgcca aatgcccgtt attgttagcg
     6421 gacacgaaaa ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg
     6481 ggttcatttc atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc
     6541 agattgaatt gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag
     6601 ttctgccgtt tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg
     6661 aaaaactaca tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca
     6721 aaataccagc gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg
     6781 tacactgatc gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg
     6841 taatgcaagt tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa
     6901 acgttaaagc gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta
     6961 ccaagaaaaa cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg
     7021 aagttctggc agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca
     7081 tcgcgtctaa agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg
     7141 ctgacgctgt aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg
     7201 gcgttctgcg taccactggc gagcacgaag tgagcttcca ggttcacagc gaagtattcg
     7261 cgaaagtgat cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc
     7321 cgaccagtgg tcggcgtttt gcttttgtag cgagcacttg cagatatcga taagggccac
     7381 aataagcagc cctacgaaga taattactta gctgcctgaa tatcagccag cattggggca
     7441 agggctgggt tgtccttagc gaagtttgcg tacattggct gtactttctc gataaacgga
     7501 gctttatcta cctggtagac tttcacgccc attttatcca gttttgcaaa ttcttgtttc
     7561 tcttcctcgc tccacaagtc tttcatgtag ctcattgatt ctttagctgc ttttaatata
     7621 actgcctgat tttccgggct aagtttatca aacgcagcat tgctcatcag aaccacatcg
     7681 ggaaccatag tgtgttcgtc aaggctgaag gattttgcca cttcaccgtg acgcatggtg
     7741 gtcagcgcca tcacgctgtt ttctgccata tcgaccacac cctgctgtaa ggctgtatac
     7801 aactcgccgt aatccagtgg cgtcgggtta ccgcccatga ctttgatcat ttcaactgcg
     7861 ccagggcttg gctggacacg gactttcatg cctttcagat cgtctggttt cagtactggt
     7921 ttattgccat agaaactgcg ggcgcctcca tcataaaaag tcagcccgaa ataacctttg
     7981 cttttcgttg agtcgaggat ttttctcccg atatcgccct gcatgacctg ataataatga
     8041 tcacgatcgc ggaacaaata aggcaggcta aataatttat aggaattttc aaatgcttcc
     8101 atttctgccg cattggtttt taccagtgga atagcgccag aacgaatcag ctccattgtt
     8161 tctcgttgag tacccaatgt tccatttgga taaatacgaa tagtaatatc accgttagag
     8221 tactctttgt tctttttcgc aaagaactcc atcgctttat gaacaggatg agacttatcc
     8281 tgattatgac tcagttttaa tgtcactgcc caggactggc ttgcggataa cataagaaca
     8341 gcaccgataa tgccagcgga tacttttatt ttcatagatg ttccttttct aataaatatg
     8401 gcggcaggtg ccgccagtga atgcgcatca gtagcccagt gcttttggca atgccatact
     8461 gatttgcgga atataggtca ccattgccag caccaatacc atcacggcat agaatggcag
     8521 cataggtttg ataattttat taatcggtaa tttagataca ctacaaccga caaataacac
     8581 actgcctaca ggcggagtac aaataccgat acacatgttc agtaccatga caataccaaa
     8641 gtgaatcggg tccatgccca gttcggtgac aacaggtaaa aatatcggcg taaaaatcag
     8701 taccgccggt gtcatatcca taaaagtgcc gataatcaac aggataatat tgataataag
     8761 caggattatt attgggttgt cagaaatagt gttaagcaaa tcgagaatca ggaagggaac
     8821 gtcagcgttt gacattgccc a
//
LOCUS       DAZPLM010000165         8797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPLM010000165 DAZPLM010000000
VERSION     DAZPLM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.165"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(7..1344)
                     /gene="adiC"
                     /locus_tag="ACHVC7_003274"
     CDS             complement(7..1344)
                     /gene="adiC"
                     /locus_tag="ACHVC7_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR2117247.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(1481..2242)
                     /gene="adiY"
                     /locus_tag="ACHVC7_003275"
     CDS             complement(1481..2242)
                     /gene="adiY"
                     /locus_tag="ACHVC7_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR2117248.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2567..4834)
                     /gene="adiA"
                     /locus_tag="ACHVC7_003276"
     CDS             complement(2567..4834)
                     /gene="adiA"
                     /locus_tag="ACHVC7_003276"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR2117249.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(5033..5941)
                     /gene="melR"
                     /locus_tag="ACHVC7_003277"
     CDS             complement(5033..5941)
                     /gene="melR"
                     /locus_tag="ACHVC7_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR2117250.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6224..7579
                     /gene="melA"
                     /locus_tag="ACHVC7_003278"
     CDS             6224..7579
                     /gene="melA"
                     /locus_tag="ACHVC7_003278"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR2117251.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7682..8533
                     /gene="melB"
                     /locus_tag="ACHVC7_003279"
                     /pseudo
     CDS             7682..8533
                     /gene="melB"
                     /locus_tag="ACHVC7_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463164.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
     gene            8627..>8797
                     /locus_tag="ACHVC7_003280"
     CDS             8627..>8797
                     /locus_tag="ACHVC7_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2117252.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGS"
BASE COUNT         2193 a         2082 c         2262 g         2260 t
ORIGIN      
        1 ggggaattaa tctttgctta ttggtgcatc taagggatac gggtttttat gtagccggtt
       61 gtaattcagc gcatacatgg cggtgatgac catcagggtg acaaatgacc acataacctc
      121 tttcgccccg gaccccacca cggcccagat gcagtagagg aaggcaatgg tagtaacagc
      181 cagatatgcc gggcgtgctt taccaaagtg accgtggccg agcagcagta acgccgcaca
      241 ggtgtaaaga tatggcacca gtgtaaagat gaccgacacg gaagatacca gaccgaactc
      301 tttggttgcg tttggcgaaa tgctactgag ctggaagatg gtcatcaaaa taccgacgat
      361 aatcagcccc gccactggcg tacccgcttt atttacacgg gcaaaaatcg gtgggaacag
      421 tccgtcatcg gcagcggctt tcgccgtttg acccgccagc aacgtccagc cgcccagtga
      481 acctaagcaa cccgcagctg cgcagaagga aacaatggcc ccagcggtgt cacccaacgc
      541 catccgtgcg gcatcaccga atggcgaggc agaaacgcgc agtgcggcat tagggatcat
      601 ccccataatc gcggtggtgg agagcacata gcaaacggcg gcaatcaata ccccaccaat
      661 ggtggcgata gggacattgc gtttcgggtt tttcaccaca cctgcggcaa cggaggcact
      721 ttccacaccg atgaacgacc acagcgtaac gttaagggta ctttgaatcg caccgaaggt
      781 gcccaggccg ctgacgttcc atgccgccat ataggtttca ccacggaacc agaaccagcc
      841 aaatacggca atcccgacga tggggatcag cgccagcacg gtggcaacgg cctgcacacg
      901 ggtgatcatt ttcggaccga caatgttcag caggacgaag atccacagca ccacgacgca
      961 ggtgatggtt aataccaatg gatctttcag aatcgggaag aagtaactta aatatcctac
     1021 gccaatgacc accatagcga tattgccgat ccagcaggcc agccagtaga ggacgttggt
     1081 ttgataaccg agaaacgggc caaagcagcg gcgggcgtaa gcgtaagaac caccaggact
     1141 tggatcgagg aacgacattt tggcgtatac catcgagagc cccagcgcac cgataatcgt
     1201 caccaaccat ccataaatgg caatcccgcc agtggaggcc aggtttgcag gtaacagaaa
     1261 aacacctgac cccataatat tccccgacac catcagggtg acggggatta agcccacttt
     1321 gtgagcatca gcatccgaag acataattaa actcctgcga aggcgagctt cgtgacaata
     1381 aattacgtca tatcatacgc ctgcattcga tagttgaatt attatcgacc gggttatcgc
     1441 tgatgccgtt attaatatag ctcttcgtta aggttaaaaa tcaggcgaca gttcgttctc
     1501 tgtgctgact aacataatgc agcggcgtca taccgtagaa gtctttaaag acagaaataa
     1561 agtacgacgt actgttgtag ccgcaggact gtgatacctg agagatattt ttaccgtcca
     1621 tcattaattc atttacggca taacgcatgc ggcaggtggt gattatttgg ctataactgg
     1681 tgttttcgct tttcagcttt ttctttaaca gacttgggct aagacataaa caactggcta
     1741 ccatactaag attccagtct ttgtgaatat cgctttcaat aatttgataa acgctgtcac
     1801 ttacacaatt acgtaacata tacatcatta atgaaaggaa ttttttactg tcgagaaaac
     1861 gggatagcac cgtgaataat aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg
     1921 gcatcatgct gtgttgcgcg gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga
     1981 gtatgggcgt agcgctacgt tgccatactg gtatttgcga gagatcttta ttcagaaacc
     2041 ggaggtaatc tttgatgatg tcgtgactaa tatgagcaac caaagtgttg ttaagagagg
     2101 agacgtcgat aatattattt tcacaattta ataacgccat atggttagct ttaaggctaa
     2161 taggttcttt cccattcacc cttatccaga catctttttc agtcaataaa acaatacaag
     2221 gttggtcgct gcaaatcctc atacatgtac tcctgagtgc gaataaaaga ccagttctcg
     2281 ctaaagcaaa gcgataccgg gaaatggtta tataattgta tgatgaatat aaacatgcat
     2341 aaaaataacg agttttacta atattacaaa tttttatagt aatttctcat gtacatattt
     2401 ttttacagga taaataacag gcaaaaaaaa gcgcggggtg gggccgcgca aaaagtatga
     2461 catttttgtt tgcagcaatt tttatgaaaa cgttgccata ctcaaaaggc ctaaggccag
     2521 gcggcgtaat gttatttaaa caattacgcc ttcagcggaa tagtggttac gctttcacac
     2581 acataacgtg gtaaataccg tcaataattt cagtcccttc agtttcgtgt tcaaatctag
     2641 ggaaatggtg gtcccaggat tgcagcgagc gtaaataact tacttgcgga ctgtttttat
     2701 cgccgaagtt ttcaccagac agcagcatcg ggattcctgg cggatatggg ataactgagt
     2761 ttgccgcgat gcgtcctggc agattttcaa tggataccag ttcgacattg ttgtcgacaa
     2821 tcgcgttgta cgcttcacgc ggggtgattt ccgccaccga caggccggaa taggcttcgt
     2881 tcaaccgtgc gccagggttg ttttctttca gccaggcaaa catggtgtca cccagatcgt
     2941 gaatccccat gttcgcgtaa gtgtcaggat attgttcaac aagttccggc atcacctgcg
     3001 ccagcggtgt gttggcgtca tagtggcgtt tgaaggagca aagggtgtta accagagttc
     3061 cccatttccc acgggttacg cccatagaga acaggaacat aatttggaag tcagtggtgc
     3121 gggtaggcac aatgccgtgg cgaccaagcc aggcagtgac cagcgccgcc ggaacaccgg
     3181 tttcttccag ttcaccatct tcacccattc ccggagcaag gatgctgact ttaatcgggt
     3241 cgagcatact ccagttatcc ggaatatctt tgaagccgtg ccagctttcg cccggatgca
     3301 ttacccagca gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca aagtcatagg
     3361 ttttgccggt ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag aaccagctac
     3421 cgtcagcggt gaactcttta tacagccgcg ccatcgcctg acggaaatca accgcttcgt
     3481 cgatcacttc ctgcgtcagt gacaggccgc tgttgccgtc catcatcgac accgccacgt
     3541 cgttggatgc gcagatggca tacagcgggg aggtggtggc atgcatcatg taggcctggt
     3601 tgaagcggga gaagttaatc gccccacgac cttcacgtac atgaatataa gaagcctgtg
     3661 aaagcgcatt cagcagtttg tgggtggagt gggtggcgaa aacggtagga ccgttgtgat
     3721 cgccaggttc gccgcgcatg gcatagtgat cggcatagat cgggttgaaa cgtgcatagc
     3781 cgtaccaggc ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga tcctgcgctt
     3841 ctttagcgtt ataacacacg ccgtcatagg tgcagttggt caccacgcag taagacggtt
     3901 tttgcccggc tttgtcttta gtcagcgggc tttcactgat tttcttctgc aaggtttcag
     3961 gttgcatttc ctgcggatag attggcccga taatgccgta gcggttgcgg cttggcacca
     4021 tgtagaccgg tttcgcgcct gtcagcatca agccttgttc gatggattta tggcagttac
     4081 ggtcaacgac cacgacatcg ttatcggtca tgcaagcctg catgatggtg cggttagagc
     4141 cggaagtacc gacgactacc gaccaggagc gatcggcacc aaatacgcgt gcggcatatt
     4201 tttcgctttc gccaaatgcg ccagtatggt caagcaaaga accgagggaa gttcgttcga
     4261 tgcccatatc ggtgcggaac agattttcac catagtagtc atggtagaaa cgtccggcgg
     4321 gtgttttggt aaaaccaacg ccgccctggt ggcctggcgc tgcccaggaa tattcatgga
     4381 tgtcgctata tttcatcagc gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg
     4441 tcatcgcggc aacagcgcgt ccggcgataa agtcggcggt atcttccaga atccaggcga
     4501 attcatcgac aagctccagc aggtcgcgat ccattgcggc gagggctttt tcccgatcgc
     4561 ccaacaggaa gaccggcacg ttttgttggc gctcatgaag cttaccgatc aattgtctga
     4621 cgttttgatg ttcgtccgga tgttccattt gatagctgaa catcaggcag tcaatggctt
     4681 cgtttgaaga gagaatggca aaaccatcat caaaggaggt ggatttaatc acggtaacat
     4741 tttgctggct taaagcatct gccagacgct caacggcgtt accgacccag gtgtcttgat
     4801 ggagaaactc gctttcaaca attaatactt tcatcattgc ttacccggtt atgaaggaat
     4861 cttcgttgcg ggcaagtatc ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac
     4921 atttttgaaa tatttatttt tataaacaat gcgttatgtg attattttaa attattttca
     4981 ctgcttatta tcgacggcta aactattttt ttggctgata ctgatatcgt ctttagccgg
     5041 gaaacgtctg gcggcgctgt tggctaagtt tgcggtattg ttgcggcgac atgccgacat
     5101 atttgccgaa cgtgctgtaa aaacgactac ttgaacgaaa gcctgccgtc agggcaatat
     5161 cgagaatact tttatcggta tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg
     5221 taatgtactg tttcatcgtc aattgcatga cccgctggaa tatccccatt gcatagttgg
     5281 cgttaagttt gacgtgctca gccacatcgt tgatggtcag cgcctgatca tagttttcgg
     5341 caataaagcc cagcatctgg ctaacataaa attgcgcatg gcgcgagacg ctgtttttgt
     5401 gtgtgcgcga ggttttattg accagaatcg gttcccagcc agagaggcta aatcgcttga
     5461 gcatcaggcc aatttcatca atggcgagct ggcgaatttg ctcgttcgga ctgtttaatt
     5521 cctgctgcca gcggcgcact tcaaacgggc taagttgctg tgtggccagt gatttgatca
     5581 ccatgccgtg agtgacgtgg ttaatcaggt ctttatccag cggccaggag agaaacagat
     5641 gcatcggcag attaaaaatc gccatgctct gacaggttcc ggtatctgtt agttggtgcg
     5701 gtgtacaggc ccagaacagc gtgatattac cctgattgat attcactttt tcattgttga
     5761 tcaggtattc cacatcgcca tcgaaaggca cattcacttc gacttgacca tgccagtggc
     5821 tggtgggcat gatatgcggt gcgcgaaact caatctccat ccgctggtat tccgaataca
     5881 gcgacagcgg gctgcgggtc tgtttttcgt cgctgctgca cataaacgta tctgtattca
     5941 tggatggctc tctttcctgg aatatcagaa ttatggcagg agtgagggag gatgactgcg
     6001 agtgggagca cggttttcac cctcttccca gagaggcgag gggagcgtcc gagcatcatg
     6061 aggccgaaaa ctctgctttt caggtaactt attaccataa actcagattt actgctgctt
     6121 cacgcaggat ctgagtttat gggaatgctc aacctggaag ccggaggttt tctgcagatt
     6181 cgcctgccat gatgaagtta ttcaagcaag ccaggagatc tgcatgatga ctgcacccaa
     6241 aattacattt atcggcgctg gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt
     6301 ccatcgcgag gcgctgaaaa cggcgcatat tgccctgatg gacattgatc ccacccgcct
     6361 ggaagagtcg catattgtgg tgcgtaagct gatggattca gcaggggcca gcggcaaaat
     6421 cacctgccac acccaacaga aagaagcctt acaggatgcc gattttgtcg tggtggcatt
     6481 tcagattggc ggttatgaac cttgcacggt gactgatttc gaggtctgta agcggcatgg
     6541 tctggaacaa accattgccg atacgttggg gccgggcggt attatgcgcg cgctacgtac
     6601 cattccgcat ctgtggcaaa tttgcgagga catgacggaa gtctgccccg atgccaccat
     6661 gctcaactac gttaacccaa tggcgatgaa tacctgggcg atgtatgccc gctatccgca
     6721 tatcaaacag gtagggctgt gccattcggt gcagggaacg gcggaagagc tggcgcgtga
     6781 cctcaatatc gatccgtcca cgctgcgtta ccgttgcgct ggtatcaacc atatggcgtt
     6841 ttacctggaa ctggagcgta aaaccgccga cggcagttac gtgaatctct acccggaact
     6901 gctggcggct tatgaagcag ggcaggcacc gaagccaaat attcatggta atactcgctg
     6961 ccagaatatt gtgcgctacg aaatgttcaa aaagctgggc tacttcgtta cggaatcgtc
     7021 agaacatttt gctgaataca cgccgtggtt tattaagcca ggtcgtgagg atttgattga
     7081 gcgttataaa gtaccgctgg atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg
     7141 gcataaagag ctggaggagt ataaaaacgc ctcccggatt gatattaaac cgtcacgaga
     7201 atatgccagc acaatcatga acgctatctg gactggcgag ccgagtgtga tttacggcaa
     7261 cgtccgtaac gatggtttga ttgataacct gccacaagga tgttgcgtgg aagtagcctg
     7321 tctggttgat gctaatggca ttcagccgac taaagtcggt acgctacctt cgcatctggc
     7381 cgccctgatg caaaccaaca tcaatgtaca gacgctgctg accgaagcta ttcttacgga
     7441 aaatcgcgac cgtgtttacc acgccgcgat gatggacccg catactgccg ccgtgctggg
     7501 cattgacgaa atatatgctc ttgttgacga cctgattgcc gcccacggcg actggctgcc
     7561 aggctggttg caccgttaaa acgcgactaa acgctactgc gccgggggat ttattccggc
     7621 gcacacctct gacgatacca ataacagaag gcgggcgttg gtaacagcga cccgataccc
     7681 tatgagcatt tcaatgacta caaaactcag ttatggattt ggagcgttcg ggaaggattt
     7741 tgcgatcggc attgtgtata tgtacctcat gtattactac accgatgtcg ttgggctgtc
     7801 tgtgggtttg gtcggtactt tgtttctggt ggcgaggatc tgggatgcta ttaacgatcc
     7861 gattatggga tggattgtaa atgctacgcg atcgcgatga ggtaagttca aaccctggat
     7921 cctgatcggt acgttggcaa actctgtaat cttatttctc ctctttagtg cgcatctgtt
     7981 tgaaggtact actcagattg tctttgtttg cgtgacctac atcctctggg gcatgactta
     8041 caccattatg gatattccct tctggtcgct ggttccaacc atcacgctcg ataaacgtga
     8101 gcgtgaacaa ctggttcctt atccgcgttt ttttgccagt ctggcgggct ttgttacggc
     8161 aggtgtgacg ctaccatttg ttaattatgt cggcggtggc gatcggggat ttggctttca
     8221 gatgttcaca ctggtactga tcgccttttt tattgtttca accatcatca ctctgcgcaa
     8281 tgtgcatgaa gtcttttcgt cagacaatca accgtctgct gaaggaagcc atctgacact
     8341 taaagccatc gttgcgctaa tttataaaaa cgatcagctt tcatgcctct tgggtatggc
     8401 tcttgcttat aatgtagcca gcaacattat taccggattt gctatctatt atttctcata
     8461 tgttatcggt gatgcggatt tgttccccta ttatctgtcg tatgcgggag ctgctaacct
     8521 ggtgacgtta gtatttagac tggccccctg aatctccaga caaccagtat cacttaaata
     8581 agtgatagtc ttaatactag tttttagact agtcattgga gtacagatga ttgatgtctt
     8641 agggccggag aaacgcagac ggcgtaccac acaggaaaag atcgcaattg ttcagcagag
     8701 ctttgaaccg gggatgacgg tctccctcgt tgcccggcaa catggtgtag cagccagcca
     8761 gttatttctc tggcgtaagc aataccagga aggaagt
//
LOCUS       DAZPLM010000166         8792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPLM010000166 DAZPLM010000000
VERSION     DAZPLM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.166"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHVC7_003281"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHVC7_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR2117253.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHVC7_003282"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHVC7_003282"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR2117254.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHVC7_003283"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHVC7_003283"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR2117255.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHVC7_003284"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHVC7_003284"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR2117256.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHVC7_003285"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHVC7_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR2117257.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHVC7_003286"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHVC7_003286"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR2117258.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHVC7_003287"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHVC7_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR2117259.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2101 a         2199 c         2380 g         2112 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa tt
//
LOCUS       DAZPLM010000167         8726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPLM010000167 DAZPLM010000000
VERSION     DAZPLM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.167"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHVC7_003288"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHVC7_003288"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR2117260.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHVC7_003289"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHVC7_003289"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR2117261.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHVC7_003290"
     CDS             complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHVC7_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR2117262.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHVC7_003291"
     CDS             complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHVC7_003291"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR2117263.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2137 a         2026 c         2231 g         2332 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaacgt cactgaagag
     4921 agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc agatttcttc
     4981 tggctactga tggttttaat actgcggttc agctttttag cgatctcggt caccaggaag
     5041 ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt gtcaccgtaa
     5101 ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa tttcttccct
     5161 ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg caccttgttt cagcacgatc
     5221 ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt catagtcaga
     5281 acaatgatcg acaggcttgg gaaatggcgc ttgatgtact tgattaaggt aatgccatcg
     5341 ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc cagtttcggc
     5401 aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac ccactcaatt
     5461 tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc aataattacg
     5521 ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     5581 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     5641 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     5701 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     5761 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     5821 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     5881 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     5941 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     6001 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     6061 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     6121 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     6181 cgggccagcg gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     6241 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     6301 cgggtccgcc tttccataac gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     6361 atccatttca tgaatactta cgccttctcc ggtgtccaga atgcggaatg tcaggcggtc
     6421 ttcagaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     6481 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     6541 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     6601 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     6661 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     6721 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     6781 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     6841 gccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     6901 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     6961 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     7021 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     7081 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     7141 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     7201 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     7261 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     7321 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     7381 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt ctttctcatt
     7441 atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     7501 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     7561 ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     7621 gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacattt ctgcacgacg
     7681 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     7741 cagggttgag atcagtacca gactgttatc ctgaccattc aggtaataca tcgaccacgg
     7801 tacattttct gcaccccaca atgtatccag ataagtggac atccgctgag tcatctcaag
     7861 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgtg gtttctccag
     7921 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     7981 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     8041 ttggattgcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     8101 ggcgctttgt accattaccc ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     8161 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     8221 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     8281 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     8341 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     8401 gcccaacgaa attaattgca aataaacgaa gattgctgga aattatgctg atgttaatta
     8461 tttgtgaaat agttgacaag agttatagtt tttctgtggt agcacagaat aatgaaagtg
     8521 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg agtaaacaga
     8581 cattcagaaa tgaatgacgg taataaataa agttaatgat gatagcggga gatattctag
     8641 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
     8701 gattttaacc ttgaattatt attgct
//
LOCUS       DAZPLM010000168         8707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPLM010000168 DAZPLM010000000
VERSION     DAZPLM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.168"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHVC7_003292"
     CDS             complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHVC7_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR2117264.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            626..844
                     /locus_tag="ACHVC7_003293"
     CDS             626..844
                     /locus_tag="ACHVC7_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR2117265.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            852..1793
                     /gene="ribF"
                     /locus_tag="ACHVC7_003294"
     CDS             852..1793
                     /gene="ribF"
                     /locus_tag="ACHVC7_003294"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR2117266.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1836..4652
                     /gene="ileS"
                     /locus_tag="ACHVC7_003295"
     CDS             1836..4652
                     /gene="ileS"
                     /locus_tag="ACHVC7_003295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR2117267.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4652..5146
                     /gene="lspA"
                     /locus_tag="ACHVC7_003296"
     CDS             4652..5146
                     /gene="lspA"
                     /locus_tag="ACHVC7_003296"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR2117268.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHVC7_003297"
     CDS             5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHVC7_003297"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2117269.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5722..6672
                     /gene="ispH"
                     /locus_tag="ACHVC7_003298"
                     /gene_synonym="lytB"
     CDS             5722..6672
                     /gene="ispH"
                     /locus_tag="ACHVC7_003298"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR2117270.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6738..7652
                     /gene="rihC"
                     /locus_tag="ACHVC7_003299"
     CDS             6738..7652
                     /gene="rihC"
                     /locus_tag="ACHVC7_003299"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR2117271.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7819..8640
                     /gene="dapB"
                     /locus_tag="ACHVC7_003300"
     CDS             7819..8640
                     /gene="dapB"
                     /locus_tag="ACHVC7_003300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR2117272.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1992 a         2166 c         2468 g         2081 t
ORIGIN      
        1 atgtgtatca caatatccac attctttgta ggggagtctg gagattgagt agatattctt
       61 gttcagaatg tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg
      121 taacggcatg tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct
      181 ctcacgcatc ataaaagcag caggcataaa aaaaacccgc ttgcgcgggc tttttcacaa
      241 agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat
      301 gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt
      361 gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata
      421 ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt
      481 cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg
      541 tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat
      601 ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg
      661 attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg
      721 tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt
      781 actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt
      841 ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag
      901 ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac
      961 agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac
     1021 ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg
     1081 aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca
     1141 ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc
     1201 gcgtaaaatt tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt
     1261 tcttgttatt acagaaagct ggcatggaat acggcttcga tatcactagt acgcaaactt
     1321 tttgcgaagg tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca
     1381 atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc
     1441 acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc
     1501 aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt
     1561 tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg
     1621 aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc
     1681 gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg
     1741 cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt
     1801 atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga
     1861 atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc
     1921 tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa
     1981 aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact
     2041 cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact
     2101 cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag
     2161 aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat
     2221 acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg
     2281 actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc
     2341 tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg
     2401 ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt
     2461 ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa
     2521 gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg
     2581 cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc
     2641 aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg
     2701 attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc
     2761 cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttacccta gatgccggta
     2821 ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat
     2881 acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc
     2941 cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg
     3001 aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc
     3061 acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag
     3121 gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc
     3181 aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca
     3241 cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta
     3301 ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt
     3361 gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg
     3421 acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc
     3481 cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt
     3541 ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg
     3601 tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca
     3661 ataccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg
     3721 tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg
     3781 ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt
     3841 ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg
     3901 taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc
     3961 acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg
     4021 acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc
     4081 agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct
     4141 tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg
     4201 gtgagtggta cgaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct
     4261 gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg
     4321 acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt
     4381 cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta
     4441 ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc
     4501 tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc
     4561 aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg
     4621 ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct
     4681 acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat
     4741 cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta
     4801 tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg
     4861 gttctttgcc ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc
     4921 gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg
     4981 caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg
     5041 cgactggcac ttcgccacct tcaaccttgc cgatactgcc atctgtgtcg gtgcggcact
     5101 gattgtgctg gaaggttttt tgccttctag agcgaaaaaa caataataaa ccctgccgga
     5161 tgcgatgctg acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag
     5221 gcgtttacgc cgcatccggc aaaaatcctc aaatataaga gcaaacctgc atgtctgaat
     5281 ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca
     5341 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc
     5401 tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct
     5461 cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc
     5521 gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa
     5581 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg
     5641 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5701 atccggcatt ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc
     5761 ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata
     5821 tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg
     5881 gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca
     5941 cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagcc gcgatttgac ggtgtttgat
     6001 gccacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc
     6061 gaagagtcga ttctcatcgg ccacgccggg catccggaag tggaaggtac aatgggccag
     6121 tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg
     6181 acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac
     6241 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6301 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6361 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6421 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa
     6481 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6541 ctggtgcaaa atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg
     6601 gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6661 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca
     6721 ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg
     6781 atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca
     6841 ccgtcgcggg taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt
     6901 tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc
     6961 gtgatgcagc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7021 accgaaagcc aatcgggata ccagcgtttc tggcgattcg tgatgccctg atgcgtgcac
     7081 cagagcctgt taccctggtg gccattggcc cgttaaccaa tattgcgctg ttactttcac
     7141 aatgcccgga atgtaaaccg tatattcgtc gtctggtgat catgggcggt tcttctggtc
     7201 gcggcaactg tacaccaaat gccgagttta atatcgctgc cgatccagaa gcggctgcct
     7261 gtgtcttccg tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa
     7321 tattaacccc tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgcttc
     7381 atgcactgtt tagccactac cgtagcggca gtatgcaaag cggtctgcga atgcacgatc
     7441 tctgcgccat cgcctggctg gtgcgcccgg acttattcac cctcaagccg tgttttgtcg
     7501 cggtagaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7561 tgggcaaacc agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt
     7621 gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca
     7681 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7741 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7801 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7861 gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc
     7921 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     7981 gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat
     8041 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8101 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt
     8161 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8221 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa
     8281 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8341 ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8401 gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt
     8461 gacatcgttg gtgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8521 cataaggcgt ccagccgtat gacatttgct aacggcgcgg taagatcggc tttgtggttg
     8581 agtggtaagg aaaatggtct ttttgatatg cgagatgtac ttaatctcaa taatttgtaa
     8641 ccacaaaata tttgttatgg tgcaaaaata acacatttaa tttattgatt ataaagggct
     8701 ttaattt
//
LOCUS       DAZPLM010000169         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPLM010000169 DAZPLM010000000
VERSION     DAZPLM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.169"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHVC7_003301"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHVC7_003301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR2117273.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHVC7_003302"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHVC7_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR2117274.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHVC7_003303"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHVC7_003303"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR2117275.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHVC7_003304"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHVC7_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR2117276.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHVC7_003305"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHVC7_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR2117277.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHVC7_003306"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHVC7_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR2117278.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHVC7_003307"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHVC7_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR2117279.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHVC7_003308"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHVC7_003308"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR2117280.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHVC7_003309"
     CDS             6614..7768
                     /locus_tag="ACHVC7_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR2117281.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHVC7_003310"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHVC7_003310"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR2117282.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZPLM010000170         8651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPLM010000170 DAZPLM010000000
VERSION     DAZPLM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.170"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            94..1737
                     /gene="eptA"
                     /locus_tag="ACHVC7_003311"
     CDS             94..1737
                     /gene="eptA"
                     /locus_tag="ACHVC7_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR2117283.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            1734..2402
                     /gene="basR"
                     /locus_tag="ACHVC7_003312"
     CDS             1734..2402
                     /gene="basR"
                     /locus_tag="ACHVC7_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR2117284.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHVC7_003313"
     CDS             2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHVC7_003313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR2117285.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHVC7_003314"
     CDS             complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHVC7_003314"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR2117286.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHVC7_003315"
     CDS             complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHVC7_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR2117287.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5446..6312)
                     /locus_tag="ACHVC7_003316"
     CDS             complement(5446..6312)
                     /locus_tag="ACHVC7_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR2117288.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHVC7_003317"
     CDS             complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHVC7_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR2117289.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2204 a         2302 c         2129 g         2016 t
ORIGIN      
        1 tccagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt gctttgcgag
       61 catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact aaaaagcccc
      121 tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat ctgcctgaat
      181 attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg attcgctgca taacgtactg
      241 gttttcttgt cgatgccggt cgtcgctttc agcgtgatta atattgtcct gacactaagc
      301 tctttcttat ggcttaatcg accactggcc tgcctgttta ttctggttgg cgcggctgca
      361 caatatttca taatgactta cggcatcgtc atcgaccgct cgatgattgc caatattatt
      421 gataccactc cagcagaaag ttatgcgctg atgacaccgc aaatgttatt aacgctggga
      481 ttcagcggcg tgcttgctgc gctgattgcc tgctggctaa aaatcaaacc tgccacctcg
      541 cgtctgcgca gtgttctttt ccgtggagcc aatattctgg tttctgtact gctgattttg
      601 ctggtcgccg cactgtttta taaagactac gcctcgttgt tccgcaataa caaagagctg
      661 gtgaaatcct taagcccctc taacagcatt gttgccagct ggtcatggta ctcccatcag
      721 cgactggcaa atctaccgct ggtgcgaatt ggtgaagacg cccaccgcaa cccgttaatg
      781 cagaacgaaa aacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag
      841 aacttctccc tcaacggcta cccgcgtgaa actaacccgc ggctggcgaa agataacgtg
      901 gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt accgtgcatg
      961 ttctcggata tgccgcgtga gcattacaaa gaagagctgg cacagcacca ggaaggcgtg
     1021 ctggatatca ttcagcaagc gggcatcaac gtgctgtgga atgacaacga tggcggctgt
     1081 aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct gcctggtcag
     1141 tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga gtacatcaat
     1201 aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg tccgacctat
     1261 tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac caatgagatc
     1321 cagacctgta ccaaagagca actggtgaac acttacgaca acacgctggt ttacgtcgac
     1381 tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt taccaccagc
     1441 ctggtttatc tttctgacca cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt
     1501 ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc cgatgctgct gtggctgtcg
     1561 gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg
     1621 caacactatt cacaagacaa tttattctcc acgctattgg gattaactgg cgttgagacg
     1681 aagtattacc aggctgcgga tgatgttctg caaacttgca ggagagtgag tgaatgaaaa
     1741 ttctgattgt tgaagacgat acgctgttat tgcagggact gattctggcg gcgcaaaccg
     1801 aaggctacac gtgcgatggc gtaacaaccg cgcggatggc ggaacaaagc cttgaggccg
     1861 gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga ctgcattttc
     1921 tcgcccgtat ccggcagaaa aaatacaccc tgccggtact gatcctcacc gctcgcgata
     1981 cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg gtgaagcctt
     2041 ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat aataatcagg
     2101 gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt caggtatgga
     2161 tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca cggttaatgc
     2221 tcaaagcagg cagtccggta catcgggaaa ttctctacaa cgacatctat aactgggaca
     2281 atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac aaagtgggca
     2341 aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat gaggaaaact
     2401 aattgaatct gatgcgtttt ctgcgccgac caatatcgct gcgccaacgg ctgatattga
     2461 ccatcggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa
     2521 gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac aacgatcgcc
     2581 acatcatgcg tgagatccgc gaggcggtcg ccagcctgat tgtccccggc gtctttatgg
     2641 tcagcctgac gctatttatc tgctatcagg cggtacgccg catcacccgc ccgctggcgg
     2701 agctgcaaaa agagctggaa gcgcgcaccg ccgacaactt aacgcccatt gccattcaca
     2761 gcgccaccct cgaaatcgaa gcggtggttt cggcgttgaa cgatctggtc agtcgcctga
     2821 cgagcacgct ggataacgaa aggttattta ccgctgacgt cgcgcacgaa ctgcgaacgc
     2881 cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac attgatgtag
     2941 caccgttagt ggcacggctt gatcagatga tggagagcgt ctcccagctg ctgcaactgg
     3001 cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg ctggaagatg
     3061 tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag caaaccctgc
     3121 tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt
     3181 tattgcgaaa cctggtagaa aacgcccatc gttacagccc acaaggcagc aacattatga
     3241 ttaagctgca agaagatggc ggagcggtca tggccgttga agatgaagga ccgggtattg
     3301 atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc cgttatggcg
     3361 ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac gggcagtttt
     3421 tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg aagaaagatc
     3481 agtacgtagc aaaccagata taaagaaagc tgctgaccac cagcacactg aacacggtag
     3541 ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg gaggagagta
     3601 tgcgcgtcag agattaagtg aaccttaaga gttcaattgg cctgataaga cagcgtcaca
     3661 tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac
     3721 gtttcgcctg caaatcggca atctcgtggt caatatcatc gattttctgc tcgatattat
     3781 cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac
     3841 ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa ccaatcaccg
     3901 ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg accagccagg
     3961 ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc gccgccagcg
     4021 cgctgtaacg gatatgcgtc gggaacatcg ctggcaaggt agaagccata acgcccgtaa
     4081 agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg atgacgttac
     4141 tgttaatcag aataaacgcc gggatcgcca acacaaacag ggcaacacta ccaagtagca
     4201 caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg acaaacagca
     4261 taccgatcat aatggcgata ataatcagca ccccgtggtc ttcggagtaa tgcaggttat
     4321 gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg gtggcaatta
     4381 ccagaccaat acatgtcaac aggctgcgcc agtatttagt ggcaatctct ttaaacgaga
     4441 ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatca acatgctgct
     4501 ggaacgccgg agtttcttcc agcgcatggc gcaggtaaag cccgataatc cctaacggca
     4561 gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg
     4621 tcgaaattaa caccaccacg cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca
     4681 gccagctgcc cataaagcca cgtttacggt caggggagta ttccgcaaca aatatcgacg
     4741 ccccggtata ttcaccgccg accgagaaac cttgtgccat cttacagatc aacagcagaa
     4801 tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag aacgtactga
     4861 tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta tcgcccaaca
     4921 taccaaagaa gagcccgcca agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg
     4981 cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct aatgcgtaag
     5041 caacaaaacc ataaacacca aaatcgaacc attccattgc attacccagt gatgctgcgg
     5101 taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt
     5161 ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc
     5221 agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta
     5281 acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca aaccagttga
     5341 cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt tgtgatcaac
     5401 ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttacg ccaaatttac
     5461 agagaagatt tcctcgacca ttgcccccac caaacgctgc gctgaacaga gtcgtggcat
     5521 ccccagcgcg actgtttgcc agcgttgggt cactgaccag caaacgggct gtttatctgc
     5581 cgtggaccac tttcccagcc aacttaaaaa atcagcatgc tcccctcccg aagaggcaaa
     5641 ccactgatgg taatagtgac gcgtggtcgg tgccgcattg atactctctg ccagatgttg
     5701 tgtcgctttc tcgcgcaaat cacccagtaa caacaccacc gcttgcgggc ttcccagacg
     5761 gcaggcatcg ccaaatgcac cccagcgcaa ctcctccaga acgataaaac agcgcccagg
     5821 caacgaccag gcgttatacg ccccagcacg ccagcgggta aaaatttgct cgctctgatc
     5881 acctgcctgc accagtaggc ctcgttgcgt taacgccttt tccttccagc gacaacgggc
     5941 tgaaaaccac tcatacaagg cggcaatttg ctgggggagc tccgggaaag cagagtgctg
     6001 gcggtgatcc attttttgca gcagcgccag atgctgcgcg atctgcgtca gcgccagatg
     6061 accgggatcc agcatcgacg ccagtttttc caccattgtc agtcgcaggc taagcggcgc
     6121 atttaacatg ccgccgttcg tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat
     6181 cagtcgttca cgaaactgct gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc
     6241 cagatccact aaaaagcggg gataaaccga ctccagcacg cgaccggggc cgtccagtaa
     6301 cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg
     6361 gcgtcggtgt aaatccagcc gtgagtcatc acagtctgct gcaattgctg ctgcatcagc
     6421 ctgaccaccg attcattttg ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat
     6481 tccgtcaaac atgaagcgaa ctcagcgcaa aaagtattca tgcctgccgt aacagattca
     6541 tcgacctgct ctgccagaac tttagccatt tgttggcaat aaagatcgac ttctgcgctt
     6601 aaagctcgtt gcaacacact gtaatcaacc gtctctgtcg gggatttctc atttccccgt
     6661 ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg
     6721 ctctgctcgt ccgttgcatc ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg
     6781 acgtggggaa ctactccgtg aaaaacagga aaatgaaacc caggacgaaa ccctgactcg
     6841 ctcaattcca ttttgacttg ttgctcaatg ggccgaataa catcggttaa cacccggcaa
     6901 agggtggatt caagctcggc aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc
     6961 gtctgtaaca gtgtctcaca gcaggtacga atctcgctta acggctccga atcatcctga
     7021 aacaaggcgg ctaactgcgc attcagcgca tcttgctgtt gacgcagaaa gtggttggcg
     7081 gaggtcaggg ccagctcgat ttcatgttta atctcgccgc tcacctgcgc ctgattgagt
     7141 tgcaatagct gcaaactttc ttcgacctga tggatatttt gccgcaattg ttcacaagcg
     7201 acgtttaacc cgtgcgcacg aaaatccagg tattcccgcg cctgctgcgc gtaattcaac
     7261 agtttatgcg cagcagatcg cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc
     7321 aacgcctgaa ttggctgggc gaacagcgaa tcttcccaca actgatcggc agcatgacga
     7381 atatgttcga ggtccgccag atcggcatga cgccagcgcc tgccgagcgc ggcatgggca
     7441 aaatcttcca cccagcgttg ttgctctggc gctggtaact taccgttgtt ggctaactca
     7501 tggcgcgctc gatttgccaa gtagccccac atcgacgaca ccggaaatat ctgctgtggc
     7561 gtaatacagc ctttcatcag cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca
     7621 ctgttacgat cctgttgatc gaacttattg accagcacat acagtggcac cgattgcccc
     7681 accgccaaaa tcgcctcacg gacctcttca tcggagatcg atttcagttg cgtataatcc
     7741 agcaccgcca gtaccgccga ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc
     7801 ggttgcccgg cttcatttgg cccgggggta tccagtaacg tcaactgacc gggataactc
     7861 tccagccccg ccagatggac aaactccact tcaatcacgg gaatatgctc aatggcggcg
     7921 taagcagaaa aaggaaaatc gacgtccagc gccttcgcca gtcgcactaa atcattcaaa
     7981 cttttcagac aatgaaaaat aggctgggca cccagataat atttttcgaa agcgacgcca
     8041 ttttcgatcc gctgcataag cgcacgcata tctttatcta tttccagcac atcggtcaga
     8101 tgcttaatat cgcaatcacg caggcgctgt tgtaattgtt gaattaaaca atcgattggc
     8161 gcgacatgtg aaaaatgcag taccggttcc ttttgcccgg gcgtatggcg aataagcgtc
     8221 ggcagcgcag tcattgggcg attacgatta ggcagaacct ccgtaccaac aatggcatta
     8281 atggtggttg atttccctgc tttcatagta ccgacaatcg ccagcaccat ttccagtcgg
     8341 gaaattttac tcaactcatt attcagcatc gcgtgacgtt cggcgatatt aggctgactc
     8401 cagggtaaag ccagttgtgg cgcgtcgtct ccgggtacag agagaggcat tttttccagt
     8461 aactgcaact gttggcgaga aagctgtaac aggcgttcag cctcctgact taactcatac
     8521 agggtctgtg tgtacataga aaattcttcc ttaaagcaaa ttttgttact tttatttagc
     8581 cagattgttt ttgagttctg tttttggctt ttataattac tgcaagaaat aattttatat
     8641 ttagtgtgtt g
//
LOCUS       DAZPLM010000171         8632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPLM010000171 DAZPLM010000000
VERSION     DAZPLM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.171"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHVC7_003318"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHVC7_003318"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR2117290.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHVC7_003319"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHVC7_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR2117291.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHVC7_003320"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHVC7_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR2117292.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHVC7_003321"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHVC7_003321"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR2117293.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHVC7_003322"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHVC7_003322"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR2117294.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHVC7_003323"
     CDS             complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHVC7_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR2117295.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHVC7_003324"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHVC7_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR2117296.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaat agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAZPLM010000172         8595 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPLM010000172 DAZPLM010000000
VERSION     DAZPLM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.172"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHVC7_003325"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHVC7_003325"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR2117297.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHVC7_003326"
     CDS             complement(1672..2667)
                     /locus_tag="ACHVC7_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2117298.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHVC7_003327"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHVC7_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHVC7_003328"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHVC7_003328"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR2117299.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHVC7_003329"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHVC7_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR2117300.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHVC7_003330"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHVC7_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR2117301.1"
                     /translation="MEQINSNKKQSSRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHVC7_003331"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHVC7_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR2117302.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..>8595
                     /locus_tag="ACHVC7_003332"
     CDS             8306..>8595
                     /locus_tag="ACHVC7_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFR2117303.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNI"
BASE COUNT         2428 a         1968 c         1937 g         2262 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgctag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaa
//
LOCUS       DAZPLM010000173         8584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPLM010000173 DAZPLM010000000
VERSION     DAZPLM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.173"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            285..1262
                     /gene="glaH"
                     /locus_tag="ACHVC7_003333"
     CDS             285..1262
                     /gene="glaH"
                     /locus_tag="ACHVC7_003333"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR2117304.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1282..2550
                     /gene="lhgO"
                     /locus_tag="ACHVC7_003334"
     CDS             1282..2550
                     /gene="lhgO"
                     /locus_tag="ACHVC7_003334"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR2117305.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2573..4021
                     /gene="gabD"
                     /locus_tag="ACHVC7_003335"
     CDS             2573..4021
                     /gene="gabD"
                     /locus_tag="ACHVC7_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2117306.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4035..5315
                     /gene="gabT"
                     /locus_tag="ACHVC7_003336"
     CDS             4035..5315
                     /gene="gabT"
                     /locus_tag="ACHVC7_003336"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR2117307.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5626..7026
                     /gene="gabP"
                     /locus_tag="ACHVC7_003337"
     CDS             5626..7026
                     /gene="gabP"
                     /locus_tag="ACHVC7_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR2117308.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7047..7709
                     /gene="csiR"
                     /locus_tag="ACHVC7_003338"
     CDS             7047..7709
                     /gene="csiR"
                     /locus_tag="ACHVC7_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR2117309.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7710..8159)
                     /gene="kbp"
                     /locus_tag="ACHVC7_003339"
     CDS             complement(7710..8159)
                     /gene="kbp"
                     /locus_tag="ACHVC7_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR2117310.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8243..8401)
                     /locus_tag="ACHVC7_003340"
     CDS             complement(8243..8401)
                     /locus_tag="ACHVC7_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR2117311.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1942 a         2231 c         2381 g         2030 t
ORIGIN      
        1 actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga taatctgttc
       61 tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc tcactttgcc
      121 ttaatactct acgttaaatg ttactaattt gttgcttttg atcacaataa gaaaacaata
      181 tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg ctacgaaatg
      241 agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat gcactgaccg
      301 ccgtacaaaa taacgctgtc gattcaggcc aggactatag cggattcact ctcatcccgt
      361 cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc aaacagtttc
      421 tcgagcaggt tgccgaatgg cccgtacagg cgctggagta caaatcgttt ctgcgttttc
      481 gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg ctgttgaaga
      541 ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc gatgatgtgg
      601 cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt ggtcgctcaa
      661 atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat gtcgataact
      721 cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac ggcacgtatg
      781 tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac atgcagggtg
      841 gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat ttccgccatc
      901 cactggcgcg tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc agcaaagatg
      961 ttttccatcc agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc tatatcgacc
     1021 agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt tccgatgcca
     1081 ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc ctgttgataa
     1141 acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat ctgcgccgtg
     1201 aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag acgcatcagt
     1261 aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg catcatcggc
     1321 atgtcgaccg ccatgcaact gattgatgtt tatccggatg cccgcattgc gttgctggaa
     1381 aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat ccatgccggg
     1441 gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcggggaa ccgcgccacc
     1501 aaagcctttt gcgatcaaaa cggcattcgc tatgacaact gcggcaagat gctggtcgcc
     1561 acgtctcagc tcgaaatgga acggatgcgc gccttatggg aacgcacagc ggcgaacggt
     1621 atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa tatcaccggg
     1681 cttggtggca tttttgtgcc gtccagcggc attgtcagct accgcgaagt cacagcggca
     1741 atggcaaaaa tcttccaggc cagaggcggc gagattattt ataacgccga agtcagcgcc
     1801 ctcagtgagc ataaaaacgg cgtggtgata cgcacccgtc agggcggtga atatgaagca
     1861 tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat gctcggcctc
     1921 gaaccgggct ttatcatctg cccgttccgt ggtgagtatt tccgccttgc gccggagcat
     1981 aaccagattg ttaaccacct gatttacccc attcccgacc ccgcgatgcc atttttgggc
     2041 gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc ggtgctggct
     2101 ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gtgacacgct ggagattttg
     2161 agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact gggcgagatg
     2221 aaaaactcgc tgtgcaaaag cggctacctg cggctggtgc aaaagtattg tccccggctt
     2281 tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt atcgccggac
     2341 ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca cacctgcaat
     2401 gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt cagcaaggta
     2461 caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc acgtagtgtg
     2521 gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa caatgaaact
     2581 taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc tggacgccaa
     2641 caatggcggg atcatcgacg tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc
     2701 gaaaatgggc gctgatgaaa cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc
     2761 ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg cgcaactggt tcaatttgat
     2821 gatggagcat caggacgatt tagcgcgcct gatgaccctc gaacagggca aaccactggc
     2881 cgaagcgaaa ggcgaaatca gctacgctgc ctcttttatt gagtggtttg ccgaagaagg
     2941 caaacgtatt tatggcgata ccattcccgg tcatcaggcc gataagcgcc tgattgttat
     3001 caagcagccg attggcgtta ccgcagctat cacgccgtgg aacttcccgg cggcgatgat
     3061 tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga agcccgccag
     3121 tcagacaccg ttctctgcgc tggcgctggc ggagctggcg attcgcgcgg gcattccggc
     3181 tggcgtgttt aacgtagtca ccggttcggc aggcgcggtc ggtaatgaac tgaccagcaa
     3241 cccgctggta cgcaaactgt cgtttaccgg ttcgaccgaa attggccgcc agttaatgga
     3301 acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat
     3361 cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct cgaaattccg
     3421 caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga
     3481 ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg cacatcggcg acgggctgga
     3541 taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca
     3601 tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag cggatgaacg
     3661 cggcggcaac ttcttccagc cgaccattct ggtggacgtg ccggccaacg ccaaagtgtc
     3721 gaaagaagag acgttcggcc ccctcgcccc gctgttccgc tttaaagatg aagccgatgt
     3781 gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc tatttctacg cccgtgattt
     3841 aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc atcgtcggca tcaataccgg
     3901 cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa gcctcgggtc tgggtcgtga
     3961 aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca tcggtcttta
     4021 actggagaat gcgaatgaac agcaataaag agttaatgca gcgccgcagt caggcgattc
     4081 cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt
     4141 gggacgttga aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg ctcaataccg
     4201 ggcacctgca tccgaaggtg gtggccgcag tggaagcgca gttgaaaaaa ctgtcgcaca
     4261 cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt atgaatcaga
     4321 aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc gaagcggtgg
     4381 aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg
     4441 gcgcgtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg aatccgtact
     4501 ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc
     4561 acggcataag cgaggatgac gctatcgcca gcatccaccg gatcttcaaa aatgatgccg
     4621 cgccggaaga tatcgccgcc atcgtgattg agccggttca gggcgaaggc ggtttctacg
     4681 ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg tgacgagcac gggatcatgc
     4741 tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc
     4801 agatgggcgt ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc
     4861 tggcgggcgt caccgggcgc gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg
     4921 gcacctatgc cggtaacccg attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg
     4981 agcatgaaaa tctgctgcaa aaagccaacg atctggggca gaagctaaaa gacggattgc
     5041 tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga gcgatgattg
     5101 ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga
     5161 tcgtggcacg cgcccgcgat aagggcctga ttcttctctc ctgcggcccg tattacaacg
     5221 tgctgcgcat ccttgtaccg ctcaccattg aagacgctca gatccgtcag ggtctggaga
     5281 tcatcagcca gtgttttgct gaggcgaagc agtagctatg tccgtttgta agagttaacc
     5341 gttgctaagg gaatgataac gatgcagtaa aaaataataa cgatgaggcg aacgacaaag
     5401 ttaaaaacga ctgcggacag tcccctcgcc cctctggaga gagggttagg gtgaggggta
     5461 aaacacgcgg ctaaaccctc ccctggccct ctccctaagg gagagggaga aaaccctccc
     5521 gctttcgttt ttaagatttt gttgaccgtc tcaattaaac gcgtgacccg gcgcgggaaa
     5581 tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg gcaatcatcg
     5641 caaccacatg agttaggcgg cgggctgaaa tcacgccacg tcaccatgtt gtctattgcc
     5701 ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga agcgggcccg
     5761 gcggtattac tggcctatct gttcgccggg ctactggtgg ttatgattat gcggatgttg
     5821 gcggaaatgg cggttgccac gcccgatacc ggttcgtttt ccacctatgc cgataaagcc
     5881 attggtcgct gggcgggcta taccatcggc tggttgtact ggtggttttg ggtactggtt
     5941 atcccgctgg aagccaatat cgccgctatg atcctgcact cgtgggttcc aggcattccc
     6001 atctggttat tttccctcgt cattaccctt gccttaactg gcagtaattt attaagcgtt
     6061 aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc tatcctggcc
     6121 tttattttcc ttggtgcagt cgcaattagc ggtttttacc cttatgccga agtgagcggg
     6181 atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc ggtattaagc
     6241 gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat tgccgccgcg
     6301 gaatccgaca cgccggaaaa acatattgtc cgcgccacca actcggttat ctggcgtatt
     6361 tctatcttct atttgtgctc tatttttgtc gtcgtggcat taataccgtg gaatatgccg
     6421 gggctgaaag ccgttggatc ttatcgctcg gttctggaat tgctcaatat tccccatgcg
     6481 aaattaatca tggactgtgt gatattactt tccgtaacca gttgcctgaa ctcggccctg
     6541 tataccgcct caaggatgct ctactcctta agccgtcgcg gtgatgctcc cgcggtaatg
     6601 ggtaaaatca accgcagtaa aaccccgtac gtggcggtgt tactctccac cggcgcggca
     6661 ttcttaacgg tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt tctgatcgac
     6721 agctccggcg ctatcgccct gctggtttat ttagtcatcg ccgtttcaca gttgcggatg
     6781 cgcaaaattc tgcgagcgga aggaagcgaa attcgcttgc gaatgtggct ttatccctgg
     6841 ctcacctggc tggtcatcgg ctttattacc tttgtgttgg tagtgatgct attccgtccg
     6901 gcgcaacagt tagaagtgat ctccaccggc ttattagcga tagggattat ctgtaccgtg
     6961 ccaattatgg ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt tcataatacg
     7021 cgctgataat gatcaggagt cacaccatga ccattacgtc tctggatggc tatcgctggc
     7081 tgaagaacga tattattcgc ggtaattttc aaccggatga aaaattacgg atgagtttgc
     7141 tgacatcgcg ttatgcactt ggcgttgggc cgttgcggga agcactttct caactggtgg
     7201 cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct atgtcagagc
     7261 aggagctgct cgatattttc gatgcccgcg ccaatatgga agcgatgtta gtgagtctgg
     7321 cgattgcccg cggtggcgat gagtgggagg cagacgttct cgcaaaagcg catctgctca
     7381 gtaagctgga agcctgtgac gccagcgaga aaatgcttga tgagtgggat ctgcgtcatc
     7441 aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg caaatgcgtg
     7501 aacggttgtt tgatctggcg gcgcgctatc gatttatctg gctgcggcga acggtgcttt
     7561 cggtagaaat gctggaggat aaacacgatc agcaccagac cctgactgcg gcggtactgg
     7621 cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg ccaattccca
     7681 ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa cacttgcccc
     7741 ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat tttattgtac
     7801 agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc agacttaacg
     7861 gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc aacactggca
     7921 ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg actcaggccg
     7981 tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc agcatcaggt
     8041 ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga
     8101 cctgtaaccg cgtcccagag tttttctccg gcatctttca caaaattgaa cagacccata
     8161 gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa
     8221 cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac
     8281 cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa ccgaaccctt
     8341 taccgagcag cacgccgagc ggcggtagaa tgatggtgat gacgattctc cagaaaccca
     8401 tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc tcttaagcgt
     8461 agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt tacgctgctg
     8521 aggctggtat atgttgcatt tttagtttag agcttactta atttagaaaa tacttaaata
     8581 aata
//
LOCUS       DAZPLM010000174         8572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPLM010000174 DAZPLM010000000
VERSION     DAZPLM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.174"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            185..1507
                     /gene="dcuC"
                     /locus_tag="ACHVC7_003341"
     CDS             185..1507
                     /gene="dcuC"
                     /locus_tag="ACHVC7_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR2117312.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1550..2047)
                     /gene="sspB"
                     /locus_tag="ACHVC7_003342"
     CDS             complement(1550..2047)
                     /gene="sspB"
                     /locus_tag="ACHVC7_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR2117313.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2053..2691)
                     /gene="sspA"
                     /locus_tag="ACHVC7_003343"
     CDS             complement(2053..2691)
                     /gene="sspA"
                     /locus_tag="ACHVC7_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR2117314.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3086..3478)
                     /gene="rpsI"
                     /locus_tag="ACHVC7_003344"
     CDS             complement(3086..3478)
                     /gene="rpsI"
                     /locus_tag="ACHVC7_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR2117315.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3494..3922)
                     /gene="rplM"
                     /locus_tag="ACHVC7_003345"
     CDS             complement(3494..3922)
                     /gene="rplM"
                     /locus_tag="ACHVC7_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR2117316.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4141..5268)
                     /gene="zapE"
                     /locus_tag="ACHVC7_003346"
     CDS             complement(4141..5268)
                     /gene="zapE"
                     /locus_tag="ACHVC7_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR2117317.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5462..5860
                     /gene="zapG"
                     /locus_tag="ACHVC7_003347"
     CDS             5462..5860
                     /gene="zapG"
                     /locus_tag="ACHVC7_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR2117318.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6014..7381
                     /gene="degQ"
                     /locus_tag="ACHVC7_003348"
     CDS             6014..7381
                     /gene="degQ"
                     /locus_tag="ACHVC7_003348"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR2117319.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            7471..8538
                     /gene="degS"
                     /locus_tag="ACHVC7_003349"
     CDS             7471..8538
                     /gene="degS"
                     /locus_tag="ACHVC7_003349"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR2117320.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2083 a         2166 c         2191 g         2132 t
ORIGIN      
        1 tgaatattat gaatgtgttc ttacaaaata atcataagcg catatttttt aatgaaaaat
       61 cacatcacct acaattaaaa acacgacatc cgcaccataa atagacttgc aaaaaatata
      121 acatcattgt tttcaatctg ccgtttatgg gattgaccgt tttcttttga cacggagttc
      181 aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct atttgatcct
      241 gaaaaactac aaacctcagg tggtgctggc tgccgcaggt gtcttcctga tgatgtgcgg
      301 agtctggtta gggttcggtg gtgtactcga tcccgccaaa agcagcggct acttgatcgt
      361 cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat tggggctgtc
      421 gattatggcg gtgggcggtt atgcccgcta catggagcgc ataggagcca gtcgcgcgat
      481 ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg ccgtatatta ttctgtcggc
      541 aacttacgtc atcggccaaa tcatggcgca gtttattacc agcgcctccg gtctgggtat
      601 gttgctgatg gtcaccttat ttccgacgct ggtgagtctg ggagtaagtc gcctctctgc
      661 ggtggcggtt atcgcaacca cgatgtccat tgagtggggg attctggaaa cgaactccat
      721 ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac ttcttccact accagcttcc
      781 ggtcgcctct tgcgtcatta tctcggtggc gatctcccac tttttcgtgc aacgcgcttt
      841 tgacaaaaaa gataaaaata tcaatcacga acaggcagag caaaaaactc tcgataatgt
      901 cccgccgctc tattacgcca ttttacctgt gatgccgtta atcctgatgc tcggctcgct
      961 gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat ctggtggtgg tgatgttact
     1021 aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag cataacttgc gcgaaacaat
     1081 ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgccaacg tggtaacgct
     1141 ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg ttgatgcggt
     1201 tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta ttatggcgct
     1261 agtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta tgtcatttgc
     1321 cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa tgattatgcc
     1381 gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg attactgcgg tggtgaagcg
     1441 gacagcgatc cccatggcag tcggtttcgt ggtgaatatg attgccacaa tcacgctatt
     1501 ttattaagtc attaaaaaga caaaacaggc cgcctgggcc tgttttgtat tacttcacaa
     1561 cacgtaatgc cggtcgacca ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt
     1621 catcatcgtg atctggcttg tcgccatcaa taaccgacat aacggtttcg ttgtctgccg
     1681 atgcctcttc atcattcatg atgctggtat cttcatcgta ggcagcttca ggctcaaaca
     1741 tcgtgcctgc gccattttca cgggcgtaga tagccagcac ggcagccagc ggcacagaaa
     1801 cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac ctcatcattc gccagttcca
     1861 gattgccgac agcacgcggc gcaatgttga gtacgatttg cccgtcacgc gcatattcca
     1921 taggaacctg cacgccaggg agcgtcacat ccaccaccag gtgcggcgtg agctggttat
     1981 ccagcaacca ctcatagaat gcacgcagca gatagggacg acgtggtgtt agctgtgaca
     2041 aatccataca gattaactcc ggcccagacg catttcacgt tctgcttcag ttaaagaagc
     2101 aaggaaagag tcacgctcaa agacgcgggt catatagcct ttcagctctt tcgcacccgg
     2161 gccgctgagc tcgataccca gttgcggcag acgccacagc agcggagcaa gatagcaatc
     2221 gaccaggctg aactcatcgc tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc
     2281 cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct tcagaagctg aaccgttgat
     2341 gatggtgttc atcagcgtgt accagtcttt ttcgatgcga tgcatgtaca gacggctttc
     2401 accgcgagct accgggtaaa caggcatcag tggcggatgc gggaaacgct catccagata
     2461 ttccataatg atgcgagatt cccacagggt cagctcacga tccaccaggg tcggaacgct
     2521 ctgattcggg ttgaggtcaa tcagatcctg aggcggattg tccttttcca cgtgttcgat
     2581 ctcgaaactt acacctttct cagccagcac aatgcggacc tgatggctat agatgtcagt
     2641 aggaccggaa aacagcgtca ttaccgaacg tttgttggca gcgacagcca tgaaaacctc
     2701 caggtatagt cagaattttt actgctacca gccaccaggt ggccagtcag aagttgtgtt
     2761 acccaataag gaacgactct ctttgttcga aaatcaaaca aaaaatgagc aatacccgac
     2821 atttgggcag aaaattggat gatagtttac cagattttgc gaccattgtg gtgagtggat
     2881 gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat agttgactta gacccttatt
     2941 ggcgatgtgg tttttgtttt gcctgtcttt cagacggcag caaaaagcaa cttcccattt
     3001 tttacgctga ttcagaattt agccataaaa aaacccgccg aagcgggttt tttcgtaaat
     3061 tgttttctgc cggagcagaa gccaattaac gtttggagaa ctgcggacga cgacgtgctt
     3121 tacgcagacc gactttctta cgttcaacct gacgagcgtc acgagtaacg aagccagctt
     3181 tacgcagttc agaacgcagg gactcgtcgt attccatcag agcgcgggtg ataccgtgac
     3241 ggatcgcacc agcctgacca gagataccac cacctttaac ggtgatgtac aggtccagtt
     3301 tctcaaccat gtcgaccagt tccagcggct gacgaactac catgcgggca gtttcacgac
     3361 cgaagtactg ttccagagaa cgttggttga ttacgatttt accgttgccc ggtttgatga
     3421 aaacgcgagc tgcggaattt ttgcggcgac cagtgccgta gtattgattt tcagccattg
     3481 cctataatcc cgattagatg tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt
     3541 tacccgcgta aactttcagt ttacggaaca tagcacgacc cagcgggcct tttggcaaca
     3601 tgcctttaac cgcgatttca atcacacgct caggacggcg agcaatcatc tcttcaaagc
     3661 tcacttgttt gataccaccg atgtggccgg tgtggtgata gtacactttg tcagtacgct
     3721 tgttgccggt tacagcaact ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta
     3781 cgtgcggagt gtattccgct ttgtgcttac cgcgtaggcg acgagccagt tcagtagcca
     3841 gacggcccag agttttaccg gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg
     3901 gtttagctgt aaaagttttc attaaaagct tacccaataa atagttacac gttggtgaac
     3961 acccaaacgt cttcaattgt tgaggttcac acgacaaagt ccggcaaacc taccccttcg
     4021 aatagcctat gccagcacac aaaaagtttt gggaaaaaaa ctttcttgta acgtggggtc
     4081 gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt gtgacaggtt
     4141 ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt tgcatctctt gcagacgtga
     4201 caggcaacgc tggaactcaa acttcagccg atcgccctga taaatttcat acagcggcac
     4261 ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa
     4321 gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca acatgaccgt
     4381 atgaaagaga cgtgagagcg caatatagtc atgctgactg cgggcgtcga cgcacagcgt
     4441 agtaaaagag accgccagcg tctggttctc gacgcccatt gtcgctaatg gccgatggtt
     4501 gatttctaac gtcggtgaat tttctcgttt cccccccgcc agcgccaacc atagtttatc
     4561 catttgcgcc cgggtttcat cgtggagtgg cgaaagccac agatgcgcct gagtgagtgt
     4621 acgcagtcga taatcaacac cagcgtccac gttcattaca tcacaatgct gtttaatggc
     4681 atcgattgca ggcagaaaac gcgcacgttg caagccattt cgataaagtt cgtccggcgg
     4741 aatatttgac gtcgctacca gggtaatacc gcgggcgaac aaggctttca tcagaccgcc
     4801 aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg tcaaaacaga gcacgtcagt
     4861 ttcggcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct gtaaggcagt
     4921 tagctcttca tgcacccgca gcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc
     4981 cggcaggctt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta caccgcccca
     5041 catatataag ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc acagcttacc
     5101 gacccgcgcc attagcccac tcgtcctggg ggctggtggc gtgctattga tgagttcctg
     5161 ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc
     5221 ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga tcttattgtt
     5281 attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc cgttctacac
     5341 tacgcgatat gcagtcggga ttccacttct gtggaattaa cggttatagt ggcataatca
     5401 gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac gggagatgtt
     5461 catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg gtgctgtggc
     5521 catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg aactggaaaa
     5581 gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg cccgcagcgc
     5641 ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca tggcgaaaag
     5701 ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta atcgtctggc
     5761 agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact attctgaagg
     5821 cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc
     5881 attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc tcattgttag
     5941 ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc attcaggtac
     6001 gagagcagga ataatgaaaa aacaacccca gctgttgagt gcattagcgt taagtgtcgg
     6061 gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg ttgccgatca
     6121 ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt
     6181 acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca aaaagttttt
     6241 tggtgatgat ttaccggagc aacctgcaca acccttcgaa ggtttaggct ccggtgtcat
     6301 catcaacgcc agtaaaggct atgtgctgac caacaaccat gtgattaatc aggcacagaa
     6361 aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg gtagcgatga
     6421 ccagagcgat atcgccctgt tacaaattca aaacccgagc aaattaacgc aaatcgctat
     6481 tgccgactcc gataaattgc gcgtcggtga ttttgccgta gcggtcggta acccatttgg
     6541 ccttgggcaa accgccacct ctggcattgt ttccgcatta ggacgcagcg ggttgaatct
     6601 tgaaggtctg gaaaacttta tccagacaga tgcttccatt aaccgcggta actccggcgg
     6661 tgcactgtta aaccttaacg gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg
     6721 cggcgggagc gtcgggattg gatttgccat ccccagtaat atggcgcgaa cactggcgca
     6781 gcagcttatc gactttggtg aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat
     6841 gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga
     6901 agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa gcgggcgata ttattaccag
     6961 cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg cgaccaccga
     7021 gccgggcaca aaagtgaaac ttggcctgct gcgtaacggc aaaccactgg aagtagaagt
     7081 gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcgcgc cagcgctgga
     7141 aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc aaaggtatta agatcgatga
     7201 agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg tgatcattgg
     7261 cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg cggcaaaatc
     7321 ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta
     7381 atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg ttaactcatg gtatgctgct
     7441 gccgttccct tttttaatga cgcctccatc atgtttgtga agctcttacg ttccgttgcg
     7501 attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg cagccttaac
     7561 ccgctttcca ctccgcaatt tgacagtacc gatgagacgc ctgccagcta taatctggcg
     7621 gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc gtggtttgaa caccaactct
     7681 cacaaccagc ttgagatccg caccctggga tccggtgtaa tcatggatca acgcggttat
     7741 atcatcacca ataaacacgt catcaacgac gccgatcaga tcatcgtcgc cttacaggat
     7801 ggtcgtgtat ttgaagcatt gctggtggga tctgactctc taaccgatct ggcggtactt
     7861 aaaattaatg ccactggcgg tttacctacc attccaatca atgcacgtcg cgtaccgcat
     7921 attggcgacg tggtactggc gatcggtaac ccgtacaacc tcgggcagac cattacccag
     7981 gggattatta gtgccacggg tcgaattggt ctgaacccga ccgggcggca aaacttcctg
     8041 caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa ctcgctgggc
     8101 gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga aacgccggaa
     8161 ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa ttatggataa gctgatccgc
     8221 gatggtcgcg tgatccgcgg ctacattggc attggcggac gtgagatcgc accgctgcac
     8281 gcacagggcg gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac
     8341 ggcccggcgg cgaatgcggg tattcaggtc aacgatctga ttatttcggt ggataacaaa
     8401 ccggccatct ctgctctgga gacgatggat caggtggcgg aaattcgccc tggttcggtg
     8461 atcccggttg tagtgatgcg tgatgataag cagttaacgc tgcaggtcac cattcaggaa
     8521 tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa ccggagtctg tg
//
LOCUS       DAZPLM010000175         8556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPLM010000175 DAZPLM010000000
VERSION     DAZPLM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.175"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(229..2220)
                     /gene="tkt"
                     /locus_tag="ACHVC7_003350"
     CDS             complement(229..2220)
                     /gene="tkt"
                     /locus_tag="ACHVC7_003350"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR2117321.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2498..3256
                     /gene="loiP"
                     /locus_tag="ACHVC7_003351"
                     /gene_synonym="yggG"
     CDS             2498..3256
                     /gene="loiP"
                     /locus_tag="ACHVC7_003351"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR2117322.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3399..4319)
                     /gene="speB"
                     /locus_tag="ACHVC7_003352"
     CDS             complement(3399..4319)
                     /gene="speB"
                     /locus_tag="ACHVC7_003352"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR2117323.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4457..6433)
                     /gene="speA"
                     /locus_tag="ACHVC7_003353"
     CDS             complement(4457..6433)
                     /gene="speA"
                     /locus_tag="ACHVC7_003353"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR2117324.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6442..6573)
                     /gene="yqgB"
                     /locus_tag="ACHVC7_003354"
     CDS             complement(6442..6573)
                     /gene="yqgB"
                     /locus_tag="ACHVC7_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR2117325.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6667..6810)
                     /gene="yqgG"
                     /locus_tag="ACHVC7_003355"
     CDS             complement(6667..6810)
                     /gene="yqgG"
                     /locus_tag="ACHVC7_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR2117326.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6921..7172)
                     /gene="yqgD"
                     /locus_tag="ACHVC7_003356"
     CDS             complement(6921..7172)
                     /gene="yqgD"
                     /locus_tag="ACHVC7_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR2117327.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7228..8382
                     /gene="metK"
                     /locus_tag="ACHVC7_003357"
     CDS             7228..8382
                     /gene="metK"
                     /locus_tag="ACHVC7_003357"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR2117328.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2090 a         2301 c         2194 g         1971 t
ORIGIN      
        1 aaaccggtgt tttgaaaaca gtgaattatt aatttcttat ataacattga gttatagata
       61 taacaacaaa tgtcaatacg catatcgtga tgcatattac ataattgata tttattgcat
      121 ataaatattt gtgatctaca acacgcttta tctattgctt gtccgcaaac ggacatatca
      181 aggtaataaa aaaggtcgcc gaagcgacct ttttacccga aatgctaatt acagcagttc
      241 ttttgctttc gcaacgacgt tgtcgacggt gaagccgaac tcttcgaaca gcaactctgc
      301 cggagctgac tcaccgaagg tggtcatacc gatgatagcg ccattcaggc caacatactt
      361 gtaccagtag tcagcaatac ccgcttctac cgcaacgcgt gcagtaaccg ctttcggcag
      421 tacggattca cggtaagccg catcctgctt gtcgaatgca tcggtagacg gcatggaaac
      481 cacgcgcgcc ttcacgcctt cggcagtcag tttttcgtag gcggcaacag ccagttcaac
      541 ttccgaaccg gtcgcgatga agatcagttc cggctgacca gcacagtctt tcagcacata
      601 accaccgcgc gcgatgtttg ccagttgctc ttcagtacgt tcctgctgcg ccagattctg
      661 acgggagagg ataagcgcgg tcggaccgtc ctgacgctca acaccgtatt tccacgcgac
      721 cgcggattca acctggtcac acggacgcca tgtagacatg ttcggggtca cgcgcagaga
      781 agcgacctgc tcaaccggct ggtgagtcgg gccgtcttcg cccagaccga tggagtcgtg
      841 ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacag cgttacgtgc
      901 gtattcgaca aacatcagga aggtggaggt gtacggcagg aagccaccgt gcagggagat
      961 accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt agttacccgc
     1021 agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg gtgccaggtc
     1081 agcggagccg ccgaggaatt ccggcaacag cgggccgaac gcttcaatag cattctgaga
     1141 cgctttacgg ctggcgattt tcgccggatt agcctgcagt ttagcgataa actctttcgc
     1201 tttggcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt cagccgcttc
     1261 ctgcggataa gctttcgcgt aagccgcaaa cttctcattc catgcagatt ctttcgcctg
     1321 gcctgcttct ttcgcatccc actgcgcata gatttcagac gggatttcga acggtgcgta
     1381 tttccagccc agctgttcgc gggtcagagc gatttctgca tcacccagcg gcgcaccgtg
     1441 ggagtcgtga gtaccggctt tgttcgggga accgaaaccg atgatggttt tgcacatcag
     1501 cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact gcgcgtttga tggatgctgc
     1561 gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa gcttcgaaac gcattgcggt
     1621 gtcgtcggtg aaccagcctt caacgtgacc atcaatagag ataccgttgt catcgtagaa
     1681 cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt gggagatgcc
     1741 ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacaa tgtcgtggcc
     1801 cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga ctgcgttggc
     1861 aataccctga cccagcggac cggtggtggt ttccacacca gcggtataac ctacttccgg
     1921 gtggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca tcggcagatc
     1981 gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt tggacagcac
     2041 gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga aatcacgcca
     2101 caggacttcg gcaatgtcag ccatacccat aggggcaccc gggtgaccgg atttggcttt
     2161 ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac gtgaggacat
     2221 tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac agcgcgtttt
     2281 gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg tacatggaac
     2341 atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa tctactcgcc
     2401 cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca aacggcgcat
     2461 ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg ccttattggt
     2521 agcaatgagc gtggcaacgg tactgactgg ttgccagaat atggactcca acggactgct
     2581 ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg tgaaaaccct
     2641 gagcgatcag gcatgtcagg agatggacag caaggcgacg attgcgccag ccaatagcga
     2701 atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc aacaatatca acggtcagcc
     2761 ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg ctaacggctg
     2821 tatccgcgtc tatagcgggc tgatggatat gatgacagat aacgaagtcg aagcggtgat
     2881 cggtcacgag atggggcacg tggcgttagg ccatgtgaag aaagggatgc aggtggcgct
     2941 tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc gggattgtcg gcagtttatc
     3001 tcaatcacaa cttggtgatc tgggcgagaa attagtcaat tcgcaattct cccagcgcca
     3061 ggaagcagaa gccgatgatt attcttacga tcttctgcgc caacgcggca tcagcccggc
     3121 aggtcttgcc accagctttg aaaaactggc gaaactggaa gaaggccgcc aaagctcaat
     3181 gtttgacgac catccggcat ccgccgaacg cgcccagcat attcgcgatc gcatgagcgc
     3241 ggatgggatt aagtaaagcc tggtggtgtc gaacgtaggt cagataaggc gttcacgccg
     3301 catctgacat caatggcaca tttgttaccc tgtgcgcatt gccggatgcg atgctgactg
     3361 catcttatcc ggcctacggg tggcatcgca tctggtgctt actcgccctt tttcgccgct
     3421 tggatataca gcatctccag cgccagcgtc gccgcagcca gagcggtgat ttccgactga
     3481 tcgtatgccg gagccacttc cactacgtcc atcccaacaa tgttgagatc tttcaggccg
     3541 cgtaccagtt taatagcgcg atcggaggtc aggccgccaa tcactggcgt accggtgcct
     3601 ggtgcaaaag caggatccag gcagtcgata tcaaaagtca ggtaaaccgg catatcaccc
     3661 acaatctgtt tcacttgggc gataacgtca tccacgctgc gatcgttcac ctggcaggcg
     3721 tccagcacgg taaagccgtt gtctttatca aactcggtac gaataccaat ctgcacggaa
     3781 tgattcgggt cgatcagacc ttctttcggc gcggtataga acatagtgcc gtggtcaaat
     3841 tcacaaccgt tcgcataggt atcggtgtgg gcgtcaaagt gtaccagcgc cattttgccg
     3901 aaatgcttcg catgagcacg cagcagcggc agcgtaacaa agtggtcacc accgaaggtg
     3961 agcatacgct taccggcagc cagcagcttc tcggcgtgcg cctgcagctt ttcgctcatc
     4021 tcacgggcat cgccaaaggc atataccaga tcgccgcagt ccacgacgtt cagacgctca
     4081 cgcatgtcga aattccacgg gaagcggttg tgttcccagg ccagattcgt cgaaacctga
     4141 cggatcgctg ccggaccgtg gcgaccaccc gcacgaccag aagtggccat atcgaacggc
     4201 acgccagtaa tcacccagtc tgcatcgctg tcatacggct ggaagttcat cggcaggcgt
     4261 aaaaaaccaa aggcattgga aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg
     4321 gatggactcc tattacaaag tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga
     4381 ggaagggttg gatttgtcac aataaattgt ggcggattat caccgctaaa ttaagcggat
     4441 tcaagtaaca caggacttac tcatcttcaa gataagtata accgtacaaa cctgcctcga
     4501 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcgc
     4561 ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg tccgccacgg
     4621 tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg aacacgtcaa
     4681 ccgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga tatgcgccga
     4741 ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
     4801 tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca caggtaatat
     4861 ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggc agaaccggga
     4921 acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa ttgacgtaca
     4981 ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca cggttttgcg
     5041 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
     5101 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc agatccatct
     5161 ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc atttcctgcc
     5221 aggtttccca catgctttgc agcgcgcgcg gcgcatcttc tgcgggcgcg gtcggcaccg
     5281 tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg tgcgcagtca
     5341 ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct tcacacgcat
     5401 cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt acggagcagt
     5461 cagactgcga acgggtgcct tcatagtcca cgcccagacc gccgccgacg tcgaagcact
     5521 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcccg
     5581 tggcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt agttgcaggc
     5641 tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga gtcgcagcca
     5701 ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc tgcgaagcca
     5761 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     5821 tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc ttctcgccaa
     5881 ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg acgctacggg
     5941 tcatgccagc atgtgccagt actgccatca actcggcttt ggaaccggct tccagaccca
     6001 gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg gtgctggtta actttgatcg
     6061 gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt ttgaacgcgg
     6121 cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     6181 gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     6241 cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag tagttattgc
     6301 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     6361 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     6421 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacgactat
     6481 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgaatgctg
     6541 acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     6601 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     6661 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     6721 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac cagaacggtg
     6781 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     6841 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     6901 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     6961 aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc cagccacgtt
     7021 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     7081 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     7141 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     7201 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     7261 tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt agacgcgatc
     7321 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     7381 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     7441 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     7501 gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga ccgtgccgat
     7561 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac taatgaaacc
     7621 gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     7681 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     7741 cagtatgacg acggcaaaat cgttggtatc gatgctgtcg tgctttccac tcagcactct
     7801 gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccgatt
     7861 ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttt
     7921 gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaattat cgttgatacc
     7981 tacggcggca tggcacgtca cggtggcgga gcattctctg gtaaagatcc atcaaaagtg
     8041 gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc tgctggcctg
     8101 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacctccatt
     8161 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     8221 gagttcttcg acctgcgccc atacggtctg attcagatgc tggatctgct gcacccgatc
     8281 tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc
     8341 gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt cacctgcttt
     8401 caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat aggcattcac
     8461 gccgcatccg gcaaaaagct gcccgcacaa taacatcatt cttcctcaac acgttttatc
     8521 gcaaattatc atcactactg aaaccgatta caccaa
//
LOCUS       DAZPLM010000176         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPLM010000176 DAZPLM010000000
VERSION     DAZPLM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.176"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(112..1776)
                     /gene="glnS"
                     /locus_tag="ACHVC7_003358"
     CDS             complement(112..1776)
                     /gene="glnS"
                     /locus_tag="ACHVC7_003358"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR2117329.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2035..3981)
                     /gene="nagE"
                     /locus_tag="ACHVC7_003359"
     CDS             complement(2035..3981)
                     /gene="nagE"
                     /locus_tag="ACHVC7_003359"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR2117330.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4314..5114
                     /gene="nagB"
                     /locus_tag="ACHVC7_003360"
     CDS             4314..5114
                     /gene="nagB"
                     /locus_tag="ACHVC7_003360"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR2117331.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5174..6322
                     /gene="nagA"
                     /locus_tag="ACHVC7_003361"
     CDS             5174..6322
                     /gene="nagA"
                     /locus_tag="ACHVC7_003361"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR2117332.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6331..7551
                     /gene="nagC"
                     /locus_tag="ACHVC7_003362"
                     /gene_synonym="nagR"
     CDS             6331..7551
                     /gene="nagC"
                     /locus_tag="ACHVC7_003362"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR2117333.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7599..8351
                     /gene="nagD"
                     /locus_tag="ACHVC7_003363"
     CDS             7599..8351
                     /gene="nagD"
                     /locus_tag="ACHVC7_003363"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR2117334.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8402..8533)
                     /locus_tag="ACHVC7_003364"
                     /pseudo
     CDS             complement(8402..8533)
                     /locus_tag="ACHVC7_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2080 a         2252 c         2178 g         2023 t
ORIGIN      
        1 catgtaggcc tgataagcgt agtgcatcag gcaatttagc gtttgaaact gtttgtctgt
       61 tccttgccgg atgcgacgta aacgccccat ccggcatagc gaaacttaaa attactcgcc
      121 tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgccgt
      181 agaatggcgg ctatcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc
      241 aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt
      301 aatcaccgac aggaaatcat ccgcagcacc agggttcggc acgctgaaca gacggtcgta
      361 cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac
      421 tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt
      481 ggtgatattg ccttcggcat ctttctccac gcgttctgcc ttaatgacgt aagcattacg
      541 cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa
      601 atcggcgcga tcgatccaaa tctcaccgct aaacggcacc tggcggctac ccatttccgg
      661 tttgttcgga tggttcggca tggtaaccat ttcgccttcg ccctgatagt tttcgataac
      721 cagtttcacc ggatcgataa ccgccattgc gcgcggggcg ttttcgttga gatcttcacg
      781 gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg
      841 tttgcagaac tcacgaatag aagcagcagt gtaaccacga cgacgcagac cggaaatggt
      901 cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt
      961 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg
     1021 aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt cctggaactc
     1081 aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc
     1141 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
     1201 gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag
     1261 gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaggttctc
     1321 ttcaacgctg cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg tgccgcggta
     1381 ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
     1441 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
     1501 gtgaaaacct aaccactcta cgtcgttttt gatcgactca acatactcga tatcttcttt
     1561 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
     1621 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
     1681 gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg
     1741 gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
     1801 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttagcgaaaa
     1861 tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
     1921 tccaccgctt ttgccagtcc cgcgtcattg ttggatgcgc gtcttatcca accaggactg
     1981 agcttttgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcataaag cagattactt
     2041 tttgatttca tacagcggtg tttgacccgc cacaacattg ccctgagctt taatgatcaa
     2101 gccactgaaa tcgtcgatat tgctgcaaac caccgggcta atcatcgaac gggcgttagc
     2161 gttcaggtaa tccagatcca tttccagaat cggttgccct gcgctcacct gcgcgccctc
     2221 ttccaccaga cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac ccatgtggac
     2281 gacaatctcc gcgccttttt cggtttctag gcagaacgcg tggttggtgt tgaagatttt
     2341 cacgattgtc cctgcggctg gtgatacgac gattttatct gtcggtttca ccgccacacc
     2401 gtcacccacc gctttgctgg cgaatgcttc gtcagaaacc tgatccagtg ccacgacatc
     2461 accggtaatc ggcgatacca gctccgcgat agataccgcg tttggtacag cctgcggttt
     2521 tgctacagga gcggcagttg ccggagttgc ttcagctgac gcagctgcta ccgggccacg
     2581 ggcaacgact ttcttcatcg catcgccaat ggattctgct ttcgcgccaa caatcacctg
     2641 aatggtctgt ttgttcagtt ttactacacc agaagcaccc agacgtttac acatcgcatc
     2701 gttgacgcga gctgagtcag ccacggtaag gcgcagacgg gtaatacagg cgtcaatcgc
     2761 tttcaggttg tcagtaccgc caaccgcagc aatatagttg gttgccagtt gagtcagacc
     2821 ttcttcagta ttgctgttag cctcttcagt tacgatctcg tcttctttat cttcacgacc
     2881 cggcgttttc aggttgaaca tgcggataac caaactgaac accacgaagt agatagcgaa
     2941 gaagacaaca cccatcacca gcagcatcca gacgttctgg ctggcggccg gcaggttata
     3001 catcaacgcg tagtcgatag cccccgcaga gaaagagaag ccagcatgga tacccagcag
     3061 cgttgccaca aacaggctga taccggtcag cagtgcgtgc aggaggtaca gcagcggagc
     3121 aaggaacatg aacaggaatt ccagcggctc agtcacaccg gtcaggaacg cagtaacagc
     3181 aacagaaagc agcatcccgc caaccatcgg acgacgctct ttcggtgctg cgaagtacat
     3241 cgccagcgcc gcacccggca gaccgaacat catgatcggg aagaagccgg acatgaacat
     3301 ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg ccgtggaaaa ccgtacccgc
     3361 cgcgttggtg aattcaccaa tctggaacca ggcgatggtg ttcagcacct gatgcagacc
     3421 ggttgggatc agcagacggt tgatgaaacc aaagataccg gaacccagcg cgcctgcaga
     3481 aacgatccac tcgccgcctg catggatagc gtgctgtact ggcggccaga cgtaaccaaa
     3541 aatggccgcc agcaccaggc agaagaagcc ggtggcaatc ggcacaaagc gtttgccgcc
     3601 gaagaagctc aggaagtccg gcagtttaat atcggaccaa cggttatagg ctgcgccacc
     3661 aaccagaccg gtaatgatac ccgccagtac acccatgtta atttctgggt tgatggtcac
     3721 catcgctttg gttaacacaa agtaacctac cgcacccgcc agcgccgccg cacctgcgct
     3781 gtctttcgac cagctggatg ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat
     3841 cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa tctggctgac cgaatcgcag
     3901 caacagtgcc gccaccggca gcaccgcgat agggagctgt aacgccctac cgagtcgctg
     3961 gaaaaaacct aaaatattca tcttattccc cctacgagaa ccctatttgg ctcgtttaaa
     4021 gccgtatttt tattttgctg caaattgtac tgccgatgtt ctgtaatcag attgttagat
     4081 catctgctac agagtgtgtg aaaatttaat tcgtatcgca aattaaacgc gtgccttttg
     4141 tgagttttgt caccaaatat cgttatcatc actccctttt actggctaaa gcagaaaact
     4201 tattttatca ttcaaaaaat caggtcggat tgacgcctgt ctgcgcaaat ccaggttacg
     4261 cttaaagatg cctaatccgc caacggctta cattttactt attgaggtga ataatgagac
     4321 tgatccccct aactaccgct gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc
     4381 gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt tctgggcctg ccgactggcg
     4441 gcacgccgat gaccacctat aaagcgttag tcgaaatgca taaagcaggc caggtcagct
     4501 ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa gagcatccgg
     4561 aaagctacta cagctttatg caccgtaatt tcttcgatca cgttgatatt ccagcagaaa
     4621 acatcaacct tctcaacggc aacgccccgg atatcgacgc cgaatgccgc cagtatgaag
     4681 aaaaaatccg ttcttacgga aaaattcatc tgtttatggg cggtgtaggt aacgacggtc
     4741 atattgcatt taacgaaccg gcgtcttctc tggcttctcg tactcgtatc aaaaccctga
     4801 ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa cgatgtcaat caggtgccaa
     4861 aatatgccct gactgtcggt gttggtacat tgctggatgc cgaagaagtg atgattctgg
     4921 tgctgggtag ccagaaagca ctggcactgc aggctgcagt tgaaggttgc gtgaaccaca
     4981 tgtggaccat cagctgtctg caactgcatc caaaagcgat catggtgtgc gatgaacctt
     5041 ccaccatgga gctgaaagtt aagactttaa gatatttcaa tgaattagaa gcagaaaata
     5101 tcaaaggtct gtaattgtta tccctgccct ggctccttgc tcagggcaat attttttaaa
     5161 atcggggggc agaatgtatg cattaaccca gggccggatc tttaccggcc acgaatttct
     5221 tgatgaccac gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga
     5281 actgccgcca gagatcgaac aacgttcact gaacggggcc attctctccc ccggttttat
     5341 cgatgtgcag ttaaacggct gcggcggcgt acagtttaac gacaccgctg aagcggtcag
     5401 cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa tcaggctgta ctaactatct
     5461 gccgacgctt atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga
     5521 gtacctggca agacatccga atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa
     5581 tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt
     5641 cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg accctggcac cggaaatggt
     5701 tcctgcggaa gtcatcagca aactggcaaa tgccgggatt gtggtttctg ccggtcactc
     5761 caacgcgacg ttgaaagaag cgaaagccgg tttccgcgcg gggattacct ttgccaccca
     5821 tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct
     5881 cgacgaagct gacatttatt gcggtattat cgctgatggc ctgcatgttg attacgccaa
     5941 cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt ctggttaccg acgccaccgc
     6001 gccagcaggt gccaacattg aacagttcat ttttgcgggt aaaacaatat actaccgtaa
     6061 cggactttgt gtggatgaga acggtacgtt aagcggttca tccttaacca tgattgaagg
     6121 cgtgcgtaat ctggtcgaac attgcggtat cgcactggat gaagtgctgc gtatggcgac
     6181 gctctatccg gcgcgtgcga ttggcgttga gaaacgtctc ggcacactcg ccgcaggtaa
     6241 agtagccaac ctgacctcat tcacacctga ttttaaaatc accaagacca tcgttaacgg
     6301 taacgaggtc gtaactcaat aagagaaagt atgacaccag gcggacaagc tcagataggt
     6361 aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat tgaccagtac
     6421 gggccaatct cgcggattca gattgccgag caaagccagc ttgcccccgc cagcgtaacc
     6481 aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc
     6541 accgggggcc gccgcgctat ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc
     6601 gtacggcttg gtcgtcacga cgccaccatc accctgtttg atctcagcag caaagtgctg
     6661 gcagaagaac attacccgct gccggaacgt acccagcaaa cgctggaaca tgccctgttg
     6721 aatgccattg ctcagtttat tgatagctac cagcgcaaac tgcgcgagct gatcgcgatt
     6781 tcggtgatcc tgccagggct tgttgacccg gacagcggca aaattcatta catgccgcat
     6841 attcaggtag aaaactgggg gctggtagaa gctctggaag aacgttttaa agtgacctgt
     6901 ttcgttggtc acgatatccg tagtctggcg ctggcggagc actacttcgg tgcaagtcag
     6961 gattgcgaag actccattct ggtgcgtgtc catcgcggaa ccggggccgg gattatctct
     7021 aacgggcgca tttttattgg ccgcaacggt aacgtcggtg aaattggcca tattcaggtc
     7081 gaaccgctgg gtgaacgctg ccactgcggc aactttggct gcctggaaac tatcgccgcc
     7141 aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc agggctacca gagccgcgtg
     7201 ccgctggacg actgcaccat caaaactatc tgcaaagccg cgaacaaagg cgatagtctg
     7261 gcgtcggaag taattgagta tgtcggtcgt catctgggta aaaccatcgc cattgctatc
     7321 aacttattta atccgcaaaa aattgttatt gccggtgaaa tcaccgaagc cgataaagtg
     7381 ctgctccctg ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt tcgtactaat
     7441 ctgccggtgg tacgttctga gctggatcac cgctcggcaa tcggcgcttt tgcgctggta
     7501 aaacgcgcca tgctcaacgg tattttgctc cagcatttgc tggaaaatta atgtgctttt
     7561 atagtggcgc ttattgttgt caatattctg ggtagtccat gaccattaaa aatgtaattt
     7621 gcgatatcga cggcgtgctg atgcacgata acgtcgccgt accgggtgca gcggaatttt
     7681 tgcacgggat tatggataaa ggcctgccgc tggtgttgct gaccaactat ccttcgcaga
     7741 ccgggcaaga tctggcgaac cgctttgcca ctgccggtgt cgatgtgccg gatagcgtgt
     7801 tttatacctc tgcgatggcg accgccgatt tcctgcgtcg ccaggaaggc aagaaagcat
     7861 atgtggtggg cgaaggcgca ctgattcatg aactgtacaa agccggtttc actattaccg
     7921 atgtgaaccc tgatttcgtg attgttggcg aaacgcgttc ctacaactgg gacatgatgc
     7981 ataaagcggc ctatttcgtc gctaacggtg cacgctttat cgccaccaac ccggacaccc
     8041 acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc agggattgag aaaatctccg
     8101 ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat catccgcgca gcattaaaca
     8161 aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga taacttgcgc accgacattc
     8221 tggcgggctt ccaggcaggt ctggaaacga ttctggtgct ttctggtgtt tcatcgctcg
     8281 acgatatcga cagtatgccg ttccgcccaa gctggattta cccgtcagtc gctgaaatcg
     8341 acgttatctg aataccaccc acgtctgcgg acgtgggtgg ctctgatgac aaacgccaaa
     8401 ctgcctgatg cgctacgctt atcaggccta cgcagctcct gcaatatatt gaattttcat
     8461 gcttttgtag gccggataag gcgttcacgc cgcatccggc atgaacaaat cgcactttat
     8521 cagcaatttg gcc
//
LOCUS       DAZPLM010000177         8431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPLM010000177 DAZPLM010000000
VERSION     DAZPLM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.177"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..253
                     /locus_tag="ACHVC7_003365"
     CDS             <1..253
                     /locus_tag="ACHVC7_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR2117335.1"
                     /translation="ATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHI
                     AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(295..1065)
                     /gene="yafV"
                     /locus_tag="ACHVC7_003366"
     CDS             complement(295..1065)
                     /gene="yafV"
                     /locus_tag="ACHVC7_003366"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR2117336.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1246..1692
                     /gene="ivy"
                     /locus_tag="ACHVC7_003367"
     CDS             1246..1692
                     /gene="ivy"
                     /locus_tag="ACHVC7_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR2117337.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1735..4179)
                     /gene="fadE"
                     /locus_tag="ACHVC7_003368"
     CDS             complement(1735..4179)
                     /gene="fadE"
                     /locus_tag="ACHVC7_003368"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR2117338.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4419..4997
                     /gene="lpcA"
                     /locus_tag="ACHVC7_003369"
     CDS             4419..4997
                     /gene="lpcA"
                     /locus_tag="ACHVC7_003369"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR2117339.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5203..5970
                     /locus_tag="ACHVC7_003370"
     CDS             5203..5970
                     /locus_tag="ACHVC7_003370"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR2117340.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5941..6681)
                     /gene="dpaA"
                     /locus_tag="ACHVC7_003371"
     CDS             complement(5941..6681)
                     /gene="dpaA"
                     /locus_tag="ACHVC7_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR2117341.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6837..7115)
                     /gene="yafQ"
                     /locus_tag="ACHVC7_003372"
     CDS             complement(6837..7115)
                     /gene="yafQ"
                     /locus_tag="ACHVC7_003372"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR2117342.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7118..7378)
                     /gene="dinJ"
                     /locus_tag="ACHVC7_003373"
     CDS             complement(7118..7378)
                     /gene="dinJ"
                     /locus_tag="ACHVC7_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR2117343.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7594..8337
                     /locus_tag="ACHVC7_003374"
     CDS             7594..8337
                     /locus_tag="ACHVC7_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2117344.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         2066 a         2225 c         2135 g         2005 t
ORIGIN      
        1 cgccacagca atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt
       61 ggtaatgaat gaagacgact gcaaaataag aagaggaaat gcagcagaat tattttcagg
      121 gatacggcac atcgctatta atattttgac gaatgataag gtattcaagg cagggttaag
      181 acgtaagatg cgaaaagcag ctatggacag aaactatctg gcgtcagtcc ttgcggagag
      241 cgggctttcg tagtcttacc ccgactctcc cccagcctta aacacaaccc ccactcaccg
      301 caacctaaac tcatccgcat cctgccatgc cggaaacttt tctctatatt cccgcaatgc
      361 catcatcgac agctccgcat caatgcgcgt tgcctgatgc gcgtcggcag tagcgataat
      421 ctcgccttgt ggattaatca cccggctgtc accgcgataa tggcagccgt tgccatcgct
      481 gccgacgcga ttgcatcccg ccacatacgc ctgattctca atcgcgcggg ccgtcagcaa
      541 tgcctgccag tgcagagagc gcggagcagg ccagttggca acgtagatgg cgaggtcata
      601 atcgttgaga ttgcgcgacc acaccggaaa acgtaagtcg tagcacacca acggcaaaat
      661 acgccagccg cgccattcca caatcactcg cgcattgccc gctttataat gtagatgctc
      721 atctgccatg cggaacagat gacgcttatc ataaaaatgt accgtgccgc ccggctcaac
      781 cagcaaaaag cggttaaccg aaccagactc cgtttgtaat gcaacactgc ctgcaatcag
      841 cgcattgcac tgctgcgcct tagctgtcat ccagtccact acgtcatctt gtgctagcga
      901 cgaagctgcc gcttccatgg caaagccgct ggtaaacatc ttcggtagaa cgatcacatc
      961 gcgcccggta ataccttcca gttgacgatc aaaatgacgc aggttggcag ggccatccat
     1021 ccacaccagt ggttgctgca aaagcgtaat cttcaaacca ggcacggtgt acaactcctt
     1081 tatgcgaagg gttttataac tttaacacct tatcaggcag ttgccttagc gcagaataaa
     1141 ttgataacaa atgctgatat tggaaatatc tgatttgcaa attatcgtgt tatcgccagg
     1201 ctttagaagg ttaataacat gggcaggata agctcgggag gaatgatgtt taagacaata
     1261 acgacagtcg ccgctctggt catcgccacc agtgcaatgg cgcaggatga tttaaccatt
     1321 agcagccttg caaagggcga aaccaccaaa gctgcattta atcagatggt acaagggcat
     1381 aagctgcctg cctgggtgat gaaaggcggt acttatactc ccgcacaaac cgtaacgttg
     1441 ggagatgata cgtatcaggt gatgagcgcg tgcaaaccgc atgactgtgg ctcgcaacgt
     1501 atcgctgtga tgtggtccga gaaatctaat cagatgacgg ggctgttctc gactattgat
     1561 gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga acgatgcgct ttcgattgat
     1621 ggtaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg aaaaccatcc ggatggcttt
     1681 aattttaaat aattagcgga taaagaaacg gagcctttcg gctccgttat tcaattacgc
     1741 ggcttcaact ttccgcactt tctccggcaa ctttaccggc ttcgtcgcca gctcttccgg
     1801 atcaaagtca tcaacgttaa tactgcgcag acggctttct tcagctttca ccagaatagc
     1861 ggcttcatct ttatcaatca gccccttcgc cagcgcgttg tgcgccagtt catccagacg
     1921 ggtaaacggc aggtttttac ccagctcttt acagatccgc tgatgaattg ggtcggcggc
     1981 aatcacatcc accagcgcct cttccagcaa gccaaccgga ttatgctcgc tcggcgtcag
     2041 gtactgaccg cgaccaatgc gggaacgggt ggcgttcggc acttgtaaaa tcttcgccac
     2101 tttatgatcc agcttgtcag aaggtgccag ataatgacgt ccggtcggga agatcaccac
     2161 attcagcagc ccggcaacca cgcggttcgg gaagttttgc agtaaatcat ccatcgcctg
     2221 ttcagcctga tacagcgcat cttgtacgcc ccagtgcacc agcggcaggt cggcttcatt
     2281 acggccttcg tcgtcataac gcttcagcac ggcagaggcg aggtagagct ggcttaaaat
     2341 atcccccaga cgggccgaga tgcgctcgcg acgtttcagg ctgccgtcca gcactgccat
     2401 cgagacatca gaaagcaggg cgaggttggc gctcaggcgg ttcaggtgct gatagtagcg
     2461 tttagtggca tcgccggttg gcgtgctgct ggttaaaccg cgcgtcaggc ccagccagaa
     2521 gctgcgaact ttgttgctac cgacgtgacc gatatgtttg aacaacagtt tatcgaacgc
     2581 gttgacgtca ttgttcttcg ccgcttccat ctcttccagc acgtacggat ggcaacgaat
     2641 cgctccttgt ccgaagatca tcatgctgcg ggtcagaatg ttagcccctt caacggtgat
     2701 ggcaatcggt gcgccctggt aagcacgcgc caggaagttg ctttgcccga gcataatgcc
     2761 tttgccacca gtgatatcca tcgcatcaat aatcgactgc tgcccgcggt gggtacagtg
     2821 atacttaacg atagccgaca gcacggcagg tttttcgccg agcataatgc cgtaggtaat
     2881 cagcgatgcc gcagcatcca tcacgtaggc attaccggca atacgcgcca gcggctcttc
     2941 aatcccttcc atcttaccga tagagatttt gaactgacgg cgaatgtgag catacgcgcc
     3001 cgttgccagc gctaccgatt tcacgccgcc ggttgagttg gaaggcaggg tgatgccgcg
     3061 gcctaccgag aggcactcca ccagcatccg ccagccttgc ccggccattt tcggcccacc
     3121 gatgatgtaa tcgatcggta cgaagacatc tttaccgcgc gtcggtccgt tctggaacgg
     3181 tacgttcagc gggaagtggc gacgaccaat ttccacgccc ggcgtggtgg tcgggatcag
     3241 cgcacaggta atacctaaat cttctgcgcc acccagcagc ttttctgggt cggagagttt
     3301 aaacgccagc ccaagcacag tcgcaatcgg tgccagcgta atatagcgtt tgttccaggt
     3361 cagacgcatc cccagcacct gctggccctg ccattcgccc atgcagacaa tcccggtgtc
     3421 cggaatcgcg cccgcatcgg aacccgcttc cgggcttgtc agtgcaaagc aagggatctc
     3481 ctgaccacgc gccagacgcg gcagatagtg atctttctgc tcgtcagtgc cgtaatgttg
     3541 cagcagctcg cccgggccta atgagttcgg cacgccgacg gtaatcgcca ggatcccgct
     3601 cacgccggag agtttttgca gcacgcgaga ctgggcataa gctgagaact ccagtccgcc
     3661 gtactctttt ttgataatca tcgcgaagaa gcgatgctct ttaaggtacg cccacaactc
     3721 cggcggcaga tccgccagct catgagtaat ctggaaatca ttcgccatcc ggcaggcttc
     3781 ttctaccggg ccgtcgagaa acgcttgctc ttcggcggtc aggcgcggct gcggatagtt
     3841 atgcagcttt ttccagtccg gcttgccctg gaacaagtcg ccctcccacc aggtggtgcc
     3901 cgcatcaatc gcttctttct cagtgcgcga catcggcggc atcaccttac ggaaaccgcg
     3961 aaataccggc gcggaaatca tcgacttacg cataggcgca aagttaaatg gcacgaggat
     4021 aatggccaga ggcaccagta cccacgctga ccacagacca gcaacgccga gggcggctgt
     4081 ccaggcgagc aaaatcagac tgctgataaa taagctcacg cggtgataga acaacgcgcc
     4141 gagcaggaca accgtagcga gaatactcaa aatcatcata acgaaaagcc ccttacttgt
     4201 aggaggtctg accacttgtg atgatatggt tgtagtggat gtaaaaacat ttagcaatgt
     4261 gtttacaata taattacaac aaagctcaca ttgttgctgt ttttatccgc acttcaggtc
     4321 aaaaagtcct ggtcatagca cctgcccgta cttctcgctt ttggcggtat ccggtacact
     4381 gcattttgtc tattacattt atgctgaagg atatcctcat gtaccaggat cttattcgta
     4441 acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt aaaagatgac gccaatattc
     4501 acgccattca gcgcgcggcg gtcctgttag cagacagctt taaagccggt ggcaaagtgc
     4561 tttcctgcgg caacggcggt tcccattgcg acgctatgca ctttgccgaa gagttgaccg
     4621 gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc tatttctgac gttagtcata
     4681 tttcctgcgt cggtaatgat ttcggtttca atgatatttt ctcccgctac gttgaagcgg
     4741 taggtcgcga aggcgatgta ctgctgggga tctccacctc cggtaactct gcaaacgtga
     4801 tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt gatcaccctg accggtaaag
     4861 acggcggtaa aatggctggc acggcggata tcgaaattcg cgttccgcac tttggttatg
     4921 ccgaccgcat tcaggagatt cacattaaag tgatccatat cctgatccag ttaattgaaa
     4981 aagagatggt taagtaagtc tggcgtaggc cggataaggc gtttacgccg catccggcac
     5041 ttgtgcgctg atgcctgatg cgacgctgat gcgtcttatc atgcctacaa atctgtacgc
     5101 gaaccgtagg ccgaataaga cgtttacgcc gcatccggcc tgaaaattct taaatcaatc
     5161 ttcgccgggg gccatgcgct cccgctgttg tggaggttac ccatgtgcga attgctcggg
     5221 atgagcgcca acgtccctac cgatatctgc tttagtttca ccggtctggt gcagcgtggt
     5281 ggtggaaccg ggccacataa agatggctgg ggcattacct tttacgaagg taaaggctgt
     5341 cgcacattta aagatccaca acccagcttt aattccccca tcgccaaact tgtccaggac
     5401 tacccgataa aatcctgttc ggtggtggct catattcgcc aggccaatcg gggcgaggtg
     5461 gcgctggaaa atactcaccc gtttacccgt gagttatggg ggcgtaactg gacttatgcc
     5521 cataacggac aactgacggg ctacaaatca ctggaaaccg gcaacttccg cccggtcggt
     5581 gaaaccgata gcgaaaaagc cttctgctgg ctcctgcata aattaacgca gcgttacccg
     5641 cgcacaccgg gcaacatggc ggcagtgttt aaatatatcg cctcactggc ggatgaactg
     5701 cggcagaagg gcgttttcaa catgctgctt tctgacgggc gctatgtgat ggcgtattgc
     5761 tcgactaatt tacactggat cacccgccgc gcgccgtttg gcgtggcaac gctgctggat
     5821 caggatgtgg aaatcgactt cagctcgcag accacaccga atgatgtggt cacggtgatt
     5881 gcaacacagc cgctgacggg caatgaaacc tggcaaaaga ttatgccagg cgaatggcgc
     5941 ttattttgcc tcggggagcg tgtagtttga tgccagttgt ggctgcacaa cttcgtggct
     6001 taacggcttg ctgaccacgt aacgaccatt gaccacagaa acggtcggtg gcttacgggt
     6061 ttgctcaaag tagtcgtaac ccggcttcag ttgctcccag aagtccttaa agttggaata
     6121 tttatggcgc ttcatattgg cgtcggtcat gcggaacggg taaatactca cttgcacgct
     6181 cggctgacca aacaccagcg caccagtaac gaactggaag atctcatcaa taccctgatt
     6241 ggtcattgcg tagcagccga tggaaacaca atcgccgtgg atcatcaggt atttcccttc
     6301 ataaccatgc gcacggtcat aggcattggg gaaaccaata ttaatcgctt tgtagtaacg
     6361 gctgtctggt tttaactgat tacgctggac gctataaaac ccttccgggc ttttgaaatc
     6421 gccctgacgc tgttttggcc ctaagccgcc ggaatattta cagattttat aactgtcgag
     6481 cagctgatat tgctcgccca ttttgacgta gagatcgagc gtacgttctt ccttgaagat
     6541 ctggatgtag acaggggatc ccatcaactg ctgcttatac tctttgctca ctggcgtggt
     6601 cgagctactg ctgcccagca aaccggcaaa cgaaacgcac gggatcaaca acatcgcaag
     6661 aattaatgcg attttacgca tactgcttat tccttgataa aacggttaca cacgccagga
     6721 cggcaaaata gatcccaaat cggaatagtc tggatttgga aggctcacat tatcaccaaa
     6781 agagttttac gcaagcctgt cggcgcaggg tttacaaatt tcatcggcag cgtgctttac
     6841 ccaaagagcg ccgcgtgagt tccagttctc tcaaatcgta aaagtttatc ggtaagtttg
     6901 taaatcagga tccagtccgg ttcgacatga gcatcgcgat aacctttcca tgaaccttgc
     6961 agcgggtggt ctttataaac agctggaagc ggtaaagcat tattgataag aagcgtcata
     7021 agatatttca atttattcat atccttatga cgcttttgtg caagttttac atcctttgaa
     7081 aattgtcccg agtattcaat atccctttga atcatattta aattcctaat ttatcaaata
     7141 aatcatcagc gtctttggcc ttatgaacat caacgccagc ttcgctgttt ttgattgatt
     7201 gaatggttaa ttgattaggc tcgcgtaaat caaacggcaa tgccttttca cgcgcgacct
     7261 ttgtgagggt tatgcgaacc aggtcagaga tggtcagccc catcccggcc agtacgtccg
     7321 ccgcctgatt cttcagatct tcatcgattc gggcgcgaac aaacgcgtta gcagccattg
     7381 ggggatctcc tgtgctgtat atttattcag cttgaattgt agctcaaatg agcaacagcg
     7441 acaatccatg agtaatttaa taaacggatg tttttacata ataaactgaa atatatttat
     7501 taattttata ataatggttt attaagtttg ttcaggtacg ggccaggata cactgcaaca
     7561 tcaatgcatg accagccatt attgagaatg tctttgccgt cgattccctc ttttgtgttg
     7621 tcgggattac tgttgctttg cttgccgttt tcttcatttg cctgcgccac cacatcacat
     7681 atctctttca gctacgccgc ccgccagcgg atgcaaaacc gtgcgcgttt attaaaacag
     7741 taccaaaccc atctgaaaaa acaggccagc tacattgtgg aaggcaatgc ccaaagcaga
     7801 agggcgctgc gccagcacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
     7861 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
     7921 agcgatcacc tgcataacat caccgaagtg gcgattcacc gtctggagca gcagcttggc
     7981 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
     8041 ttttatgcct acaacaagat cctcgaggcg aagctcccgc gtacggccaa tgagatgtat
     8101 cactaccgtc gggcaaccat tgtggcgaat aacgacctgc gccggggaga tttgctgttt
     8161 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
     8221 tttattgagt caccgcgcac cggggaaacc attcgggtaa gtcggttagc tgaacctttc
     8281 tggcaggatc attttttggg cgcgcggcgg atattgacgg aagagacgat tttgtaggac
     8341 ggataaggcg tttacgccgc atccggcggt ggtgcgcaga tgcctgatgc gacgctgacg
     8401 cgtcttatca tgcctacaaa tctgtgcgtt t
//
LOCUS       DAZPLM010000178         8412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPLM010000178 DAZPLM010000000
VERSION     DAZPLM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.178"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHVC7_003375"
     CDS             <1..189
                     /locus_tag="ACHVC7_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117345.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            412..909
                     /gene="ubiC"
                     /locus_tag="ACHVC7_003376"
     CDS             412..909
                     /gene="ubiC"
                     /locus_tag="ACHVC7_003376"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR2117346.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            922..1794
                     /gene="ubiA"
                     /locus_tag="ACHVC7_003377"
     CDS             922..1794
                     /gene="ubiA"
                     /locus_tag="ACHVC7_003377"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR2117347.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHVC7_003378"
     CDS             complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHVC7_003378"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR2117348.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHVC7_003379"
     CDS             4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHVC7_003379"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR2117349.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5021..5629
                     /gene="lexA"
                     /locus_tag="ACHVC7_003380"
     CDS             5021..5629
                     /gene="lexA"
                     /locus_tag="ACHVC7_003380"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR2117350.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5702..7027
                     /gene="dinF"
                     /locus_tag="ACHVC7_003381"
     CDS             5702..7027
                     /gene="dinF"
                     /locus_tag="ACHVC7_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR2117351.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7143..7352
                     /locus_tag="ACHVC7_003382"
     CDS             7143..7352
                     /locus_tag="ACHVC7_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR2117352.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHVC7_003383"
     CDS             complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHVC7_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR2117353.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8227..>8412
                     /gene="yjbL"
                     /locus_tag="ACHVC7_003384"
     CDS             8227..>8412
                     /gene="yjbL"
                     /locus_tag="ACHVC7_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR2117354.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIA"
BASE COUNT         1949 a         2022 c         2275 g         2166 t
ORIGIN      
        1 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
       61 cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt
      121 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata
      181 ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg
      241 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
      301 atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
      361 aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac
      421 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg
      481 caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga
      541 aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa
      601 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
      661 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg
      721 gagctggcgt tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg
      781 acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc
      841 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
      901 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
      961 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     1021 tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc
     1081 gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag
     1141 tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag
     1201 aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg
     1261 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg
     1321 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     1381 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     1441 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac
     1501 gatgatgtga agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg
     1561 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     1621 ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa
     1681 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     1741 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     1801 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     1861 gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt
     1921 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     1981 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     2041 ccggttcggc gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     2101 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     2161 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     2221 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     2281 gctgtagggg gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     2341 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     2401 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     2461 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     2521 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca
     2581 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     2641 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     2701 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     2761 gctgacgtga tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt
     2821 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     2881 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     2941 aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct
     3001 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     3061 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     3121 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     3181 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     3241 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     3301 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     3361 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     3421 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     3481 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     3541 gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     3601 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     3661 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     3721 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     3781 agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat
     3841 ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     3901 acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc
     3961 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     4021 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt
     4081 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     4141 gcgtgccgtc gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg
     4201 cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     4261 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     4321 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     4381 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     4441 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     4501 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     4561 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     4621 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     4681 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     4741 atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     4801 taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt
     4861 atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     4921 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     4981 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     5041 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     5101 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     5161 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     5221 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     5281 gcttctggcg cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     5341 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     5401 tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     5461 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact
     5521 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     5581 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     5641 gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     5701 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     5761 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     5821 tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     5881 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     5941 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg
     6001 ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg
     6061 tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc
     6121 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     6181 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt
     6241 gatggggctg catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc
     6301 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     6361 cgaaatgtcg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     6421 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc
     6481 gcgactgggg agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac
     6541 cgcctatgcg ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg
     6601 tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat
     6661 cgtagcgtta ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     6721 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     6781 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     6841 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     6901 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     6961 cgggctttct ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc
     7021 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     7081 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     7141 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     7201 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     7261 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     7321 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     7381 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     7441 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     7501 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     7561 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg
     7621 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     7681 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     7741 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     7801 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     7861 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     7921 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     7981 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta
     8041 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     8101 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     8161 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     8221 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     8281 aatcaattcg ataatgaaga tgcaatcaaa gataaattag ataatagagg ttcaatcaaa
     8341 ggtaaattaa ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat
     8401 ataattattg ca
//
LOCUS       DAZPLM010000179         8408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPLM010000179 DAZPLM010000000
VERSION     DAZPLM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.179"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /gene="yneK"
                     /locus_tag="ACHVC7_003385"
     CDS             complement(<1..171)
                     /gene="yneK"
                     /locus_tag="ACHVC7_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR2117355.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATK"
     gene            complement(248..1129)
                     /locus_tag="ACHVC7_003386"
     CDS             complement(248..1129)
                     /locus_tag="ACHVC7_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2117356.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            1230..2618
                     /gene="sad"
                     /locus_tag="ACHVC7_003387"
     CDS             1230..2618
                     /gene="sad"
                     /locus_tag="ACHVC7_003387"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR2117357.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2682..3608
                     /gene="glsB"
                     /locus_tag="ACHVC7_003388"
     CDS             2682..3608
                     /gene="glsB"
                     /locus_tag="ACHVC7_003388"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR2117358.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3608..3967
                     /locus_tag="ACHVC7_003389"
     CDS             3608..3967
                     /locus_tag="ACHVC7_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR2117359.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4106..5056
                     /locus_tag="ACHVC7_003390"
     CDS             4106..5056
                     /locus_tag="ACHVC7_003390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR2117360.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            5283..6731
                     /gene="uxaB"
                     /locus_tag="ACHVC7_003391"
     CDS             5283..6731
                     /gene="uxaB"
                     /locus_tag="ACHVC7_003391"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR2117361.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            6791..7000
                     /locus_tag="ACHVC7_003392"
                     /pseudo
     CDS             6791..7000
                     /locus_tag="ACHVC7_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
     gene            7185..7286
                     /locus_tag="ACHVC7_003393"
                     /pseudo
     CDS             7185..7286
                     /locus_tag="ACHVC7_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            7647..8184
                     /locus_tag="ACHVC7_003394"
                     /pseudo
     CDS             7647..8184
                     /locus_tag="ACHVC7_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            8226..>8408
                     /locus_tag="ACHVC7_003395"
     CDS             8226..>8408
                     /locus_tag="ACHVC7_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117362.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
BASE COUNT         2097 a         1930 c         2170 g         2211 t
ORIGIN      
        1 ttttgtggct gagttgatgt ttaaagacgt gcgccactct tcatcacata ttattgtact
       61 tagcgcatca ataaagtcct ttaaaacttc ttttacctgt ggtgcaatat tacgaacggg
      121 aaaatcatca ggaagagata tgtaattgct gatgcttact ggcgtaacca tatttgcccc
      181 atatagtcat aagtcatcgg aacatcacta tatagagcat attagccgca agaactatca
      241 aaatagttca ttggtaactc tgttttgccg agtcagacac atccagtagt tgaataaatg
      301 cctcaagcgg acgcgttttc gcaccgcgac gccagaccag ccaggttgtt aaccaacgcc
      361 attgctcagc taacggccac gcttcaacct ggtgatgccc cggcatactt tccagcatag
      421 agcgtggaat aagcgcaatg cctgctcctg cgaccacaca ggccaacatc ccgtgatacg
      481 actccatctc gtgaatagtt cccggagcgg caccgtcagc atgaaaccag ctctcgaagt
      541 ggcgacgata cgaacaattg gtgcggaagg cataaatgtt actgccgtta acctgactgg
      601 cgcggattac tggcgcatga ccttgtggcg tgacgatcat cagctcctcg cggtataccg
      661 gtatcccgtc aatggcagta tggttaatgg gcccatcaat aaatgccgca ttcagttttc
      721 cctccagcac tccttccagc atagtgccgg aagggccggt ggaaagtgaa aactgaattt
      781 tgggataacg atggttgaat tccgccagcg tggccggaat gcgcactgct gcggtgcttt
      841 ccagcgaacc aagagaaaat aaaccttgcg gctcatcgcc agcgacaacg ctccgcgctt
      901 catccactaa cgcgagaatt tgttggctat agcgtaaaaa gttatgcccg gcaggagaga
      961 gacgtaaacg ctgattctcg cgaataaaca gatctacccc cagttctgtt tccagctggc
     1021 gtagacgggt agtgagattc gacggtacgc gatgcacttt tgctgcagcc tgggtaatgc
     1081 tgccagcttc ggcaaccgcg ttgaacatct ccagttgggt cagatccata acattctcga
     1141 ttcgtgaata agtggcttaa tattattcat tttaaagcaa gagtaaatct gcgtatcttc
     1201 ataccatgac tcataaagga gataccccga tgaccattac tccggcaact catgcaattt
     1261 caataaatcc tgccacgggt gaacaacttt ctgtgctgcc gtgggctggc gctaacgata
     1321 tcgaaaacgc acttgagctg gcggcagcag gctttcgcga ctggcgcgag acaaatatag
     1381 attatcgtgc tgaaaaaatg cgtgatatcg gtaaggctct gcgcgcccgt agcgaagaaa
     1441 tggcgcaaat gatcacccgc gaaatgggca aaccaatcaa ccaggcgcgc gctgaagtgg
     1501 cgaaatcggc gaatttgtgt gactggtatg cagaacatgg tccggcaatg ctgaaggcgg
     1561 aacctacact ggtggaaaat cagcaggcgg ttattgagta tcgaccgttg gggacgattc
     1621 tggcgattat gccgtggaat tttccgttat ggcaggtgat gcgtggcgct gttcccatca
     1681 ttcttgcagg taacggctac ttacttaaac atgcgccgaa tgtgatggga tgtgctcagc
     1741 tcattgccca ggtgtttaaa gatgcgggaa tcccgcaagg cgtatatggc tggctgaatg
     1801 ccgacaacga cggtgtcagt cagatgatta aagactcgcg cattgctgct gtcacggtga
     1861 ccggaagtgt tcgtgctgga gcggctattg gcgcacaggc tggagcggca ctgaaaaaat
     1921 gcgtactgga actgggcggt tcggatccgt ttattgtgct taacgatgcc gatctggaac
     1981 tggcggttaa agcggcggta gccggacgtt atcagaatac cggacaggta tgtgcagcgg
     2041 caaaacgctt tattatcgaa gagggaattg cttctgcatt taccgaacgt tttgtggcag
     2101 ctgcggcagc cttgaaaatg ggcgatcccc gtgacgaaga gaacactctc ggaccaatgg
     2161 ctcgttttga tttacgtgat gagctgcatc atcaggtaga gaaaaccctg gcgcagggtg
     2221 cgcgtttgtt actgggcggg gaaaagatgg ctggggcagg taactactat ccgccaacgg
     2281 ttctggcgaa tgttacccca gaaatgaccg cgtttcggga agaaatgttt ggccccgttg
     2341 cggcaatcac cgttgcgaaa gatgcagaac atgcgctgga actggctaat gatagtgagt
     2401 tcggcctttc agcgaccatt tttaccaccg acgaaacaca ggccagacag atggcggcac
     2461 gtctggaatg cggtggggtg tttatcaatg gttattgtgc cagcgacgcg cgagtggcct
     2521 ttggtggcgt gaaaaagagt ggctttggtc gtgagctttc ccatttcggc ttacacgaat
     2581 tctgtaatat ccagacggtg tggaaagacc ggatctgacg cgacatattc agctctgata
     2641 tactcgcagg tcttttcaga cctgcggtcc aggagtagaa agtggcagtc gccatggata
     2701 atgcaatttt agaaaacatc ttgcggcaag tgcggccgct cattggtcag ggtaaagtcg
     2761 cggattatat tccggcgctg gctacagtag acggttcccg attgggcatt gctatctgta
     2821 ccgttgacgg acagcttttt caggccggag acgcgcaaga acgtttttcc attcagtcta
     2881 tttccaaagt gctgagtctc gttgtcgcca tgcgtcatta ctccgaagag gaaatctggc
     2941 aacgcgtcgg caaagatccg tctggatcac cgttcaattc tttagtgcaa ctggaaatgg
     3001 aacagggtat tccgcgtaat ccgtttatta atgctggtgc gctggtggtc tgcgatatgt
     3061 tgcaagggcg attaagcgca ccacggcaac gtatgctgga agtcgtgcgc ggcttaagcg
     3121 gtgtgtctga tatttcatac gatacggtgg tagcgcgttc cgaatttgaa cattccgcgc
     3181 gaaatgcggc tatcgcctgg ctgatgaagt cgtttggcaa tttccatcat gacgtgacaa
     3241 ccgttctgca aaactacttt cattactgcg ctctgaaaat gagctgtgta gagctggctc
     3301 ggacgtttgt ctttctggct aatcagggga aagctattca tattgatgaa cctgtagtga
     3361 cgccaatgca ggcgcggcaa attaacgcgc tgatggcgac cagtggtatg taccagaacg
     3421 cgggggagtt tgcctggcgg gtgggactac cggcgaaatc tggcgttggt ggcggtattg
     3481 tggcgattgt tccgcatgaa atggccatcg cagtctggag tccggaactg gatgatgcag
     3541 gtaactcgct tgcgggtatc gccgtccttg aacaattgac gaaacagtta gggcgttcgg
     3601 tttattaatg cagtctctcg atccactctt cgcgcgttta tcccgttcaa aatttcgctc
     3661 tcgctttcgt ctgggcatga aagagcgtca gtattgcctg gagaaaggcg cgccagtcat
     3721 cgaacaacat gcggcggatt ttgtcgctaa acgtcttgct ccggcattac cggctaatga
     3781 cggtaagcaa acccccatgc gcgggcatcc ggtgtttatc gctcagcacg ccaccgcgac
     3841 ctgctgtcgc ggttgtctgg ctaagtggca taacattccg cagggtgttt cgttaagcga
     3901 agaacaacaa cgttatatcg tggcggtaat ctatcactgg ctggttgtac agatgaatca
     3961 gccatgagaa ttgcggaaaa cgataaaata gcattcccag ggtaccttta tttgccgccg
     4021 ctgttagcga acatgctatc ctttatgaga atttacttat tacgttgaga taattgccac
     4081 ccgcaattgt atggacagat aattaatgca tgtacacccc atttcaactt tccgtttgtt
     4141 ccaggaaggt catctgctac gtaatagcat cgctattttt gcgctaacca cgctgttcta
     4201 ttttattggt gcagagttac ggctggttca cgaactttct cttttctggc cgctgaatgg
     4261 cgtaatggcg ggggggtttg cccgctatgt ctggcttaat cgactgcatt actatgcgat
     4321 cagttatgtg gcgatgctgg tttatgatgc cataaccacc gaatggggac tggtttcact
     4381 ggttatcaat ttctccaata tgatgtttat tgttaccgtc gccttactgg tcgtgcggga
     4441 taagcgtctg gggaaaaata agtatgagcc agtaagtgcc ttacggctat ttaattactg
     4501 tctgattgcc gcattattat gcgctattgt cggggcgatt ggttcggtca gtattgatag
     4561 tctggatttc tggcctttgc ttgcggactg gttcagtgag caattctcaa cgggcgtgtt
     4621 gatcgtgcct ctgacggtca tgctgcttga tatcgactat ttcaaaagca ttaacgataa
     4681 ctatggacat gaatgtggcg ataaagtgtt aagcgtgttt gcccggcata ttcagaagat
     4741 tgtcggtgat aaggggctgg tggcgcgcat gggcggcgaa gaatttgctg ttgcagtgcc
     4801 gtcggtgaat cctgtcgatg gtctgctaat ggcggaaaaa atccgtaaag gcgttgaact
     4861 gcaaccattc acctggcaac aaaaaacgct ttatctcacg gtaagtattg gcgtcggtag
     4921 tggtcgcgca tcgtaccgaa cgctgaccga tgattttaat aaattgatgg tcgaagccga
     4981 tacgtgtctg tatcgctcga agaaagatgg gcgcaaccgt accagcacta tgcgttacgg
     5041 tgaggaagtt gtctgacatc tttattcatt ccgcgcgatt tgaatgcaat gtgttctacc
     5101 actttatgat ccaggaaaag gcgtatttcc tcgattaaac catgatccgc gccacacttt
     5161 tctcataaaa ctgctcatta atcatcagga gttgatcaaa acaggggttc ggcgtattaa
     5221 agtttgctaa cctgtcgtac caattcaaac aggttgtatg actaatcaga agggaaccca
     5281 ttgtgaaaac actaaatcgt cgcgattttc ccggtgcaca gtatccagaa cgtatcattc
     5341 agtttggtga aggtaacttc ctgcgcgcct ttgttgactg gcaaatcgat ctcctgaatg
     5401 agcacaccga tctgaattct ggcgtggtcg ttgttcgtcc gattgaaact tcattcccgc
     5461 cgtcactgag cacgcaggat ggtctgtata ccaccattat ccgtggcctg aatgagaagg
     5521 gggaagcggt cagcgacgcg cgtctgattc gctcggttaa tcgtgaaatc agcgtctaca
     5581 gtgaatacga tgaattcctg aaactggcgc acaacccgga aatgcgcttt gttttctcta
     5641 acaccaccga agcggggatc agctaccatg cgggcgataa attcgatgac gcgccagcga
     5701 taagctatcc ggcaaaactg acccgtctgc tgttcgaacg ctttagccat ttcaacggtg
     5761 cgctggataa aggttggatc atcatcccgt gtgagttgat cgactacaat ggcgacgcgt
     5821 tgcgtgaact ggtgctgcgc tatgcgcaag agtgggcact gccagaagca tttattcaat
     5881 ggctggatca ggctaacagc ttctgttcta cgctggtgga ccgtatcgtt accggttatc
     5941 cgcgcgatga agtggctaaa ctggaagaag aactgggtta tcacgatggt ttcctcgaca
     6001 ccgctgaaca cttttacctg tttgtgattc aggggccgaa atccttagcg actgagctgc
     6061 gcctggacaa atatccgctc aacgtgctga ttgttgacga tattaaaccg tataaagaac
     6121 gcaaagtggc aatcctcaac ggtgcgcaca ccgcgctggt gccggtggcg ttccaggcag
     6181 ggctggatac cgtaggtgaa gcgatgaacg atgctgaaat ttgcgcattc gttgaaaaag
     6241 ccatctacga agaaattatt ccggtactgg atttgcctcg tgatgaactg gaatctttcg
     6301 ccagtgcagt caccgggcgt ttccgtaacc cgtataagca tcagctgctg tctatcgcgc
     6361 tcaacggtat gaccaagttc cgcacccgca tcctgccgca gctgctggca gggcagaagg
     6421 caaacggcac acttccggcg cgccttactt tcgcattagc ggcattgatt gcgttctatc
     6481 gtggtgagcg taacggagaa acatatccgg tacaggacga tgcacactgg ctggaacgtt
     6541 atcagcaact ctggagccag catcgtgacc atgttatcgg tacccaggaa ctggtagcga
     6601 ttgtgttggc agaaaaagac cactgggagc aggacctgac gcaagtgcct ggtctggtcg
     6661 agcaggttgc taatgacctg gatgcgattc ttgaaaaagg gatgcgcgaa gctgtacgtc
     6721 cgttgtgcta ataacaccgt tacttctgaa cccggttttg accgggtttc ttatttcact
     6781 atattagtta caacattttt tggcgacagc aaaggtgctg agattcccgg aagtgcagat
     6841 gcatgaaatc ctaagtgact ttgccagaat gattccggca gcactggata acgtgaagac
     6901 ttcattaccg acagattttc cggagaacgt ggtgacggca gttgaaacca atgtgttgag
     6961 gttgcatgga cggttaagcc gagaatacgg tagtaagtga gatatggggt ttggtcgttg
     7021 ttaatgagca atcatgaccg gcccaacgac tggattctcc cttggtttat gtttgtgcgc
     7081 ataatgtata ttatgttaaa tcatgtctga gtgatgcgaa gtgcttttct ggtagtcgtt
     7141 attcgttcag attaggtgtc cttgtgattt aaagatgtta tcgtggcacg aatatactgc
     7201 cattccaggc tcgttatatt gctctcgggc agtccgtagc tggtactgca aacgctgatg
     7261 cgaccttcaa agttcaatat ctataattca atcttaaggt ggctatacgt catctgaaat
     7321 tgaaacaggg tgtgtgtggc atggctacgc acacccgagc aattcaccaa tgtgaatctc
     7381 attattaatg aggagtggtt tgtatttgga gcgctatgaa aaatcacata atatctaaac
     7441 tatcaataaa caacagtagt taaacttact gttatttaaa atatttactt tgtagcaagg
     7501 ttttcctatt aaaagagaac caacatttat tacaaacgca ttaagtctgg aaacccggcg
     7561 actcgccaat gtattagcaa cattaatcga ttgtctgatg taacggtttt cccgcttatc
     7621 ctcttaacac atggagaatg catcagatgc aaactacgcg taccccctac tctatttcct
     7681 ttatggcgac agtgttactt ttattgctct ttgcttgcca tagcaccgtt gccaacgcag
     7741 ctgtcgcact ggaatacgct gcggataatc aacgctacta atcatcagtt accaggcgac
     7801 cgtgaaagcc tgttctgggt gaatgttaaa gctattcctg ctatggaaaa agaccagaag
     7861 aatgagaata cattacagct ggcaattatt agccgcataa aaatgtttta tcgccccacg
     7921 aacctggcaa tggcacctga agaagcgcct gcaatgcttc ggtttcgtcg tagcggtagc
     7981 aaactcactc ttattaatcc aacgccctat ttcattaccg taacaaatat gaaagcagga
     8041 aatagcaatc tcccaaacac catggtgccg ccaaaaggtg aagtatcggt ggatatcccg
     8101 catgctgcaa ccggggatat cagttttcag acgatcaatg attacggtgc attaactccg
     8161 cgtattaagg caaccatgca gtaagccagt gacctaatta ataataaact caatcggaat
     8221 taataatgac agcctttcgt gccgcattta aggcatatcg tatgcatcag gtgttaatac
     8281 tgcctcgctt tgcacggctt accattgccc ttggcttagc aactgcggta tttcccgttg
     8341 acgcagagta ttattttaat ccacggtttt taagcaatga tcttgctgaa tctgtcgatt
     8401 tatccgcc
//
LOCUS       DAZPLM010000180         8396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPLM010000180 DAZPLM010000000
VERSION     DAZPLM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.180"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            112..723
                     /gene="ruvA"
                     /locus_tag="ACHVC7_003396"
     CDS             112..723
                     /gene="ruvA"
                     /locus_tag="ACHVC7_003396"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR2117363.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            732..1742
                     /gene="ruvB"
                     /locus_tag="ACHVC7_003397"
     CDS             732..1742
                     /gene="ruvB"
                     /locus_tag="ACHVC7_003397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR2117364.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHVC7_003398"
     CDS             complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHVC7_003398"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR2117365.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHVC7_003399"
     CDS             complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHVC7_003399"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR2117366.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3505..4437
                     /gene="znuA"
                     /locus_tag="ACHVC7_003400"
     CDS             3505..4437
                     /gene="znuA"
                     /locus_tag="ACHVC7_003400"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR2117367.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4453..5775
                     /gene="mepM"
                     /locus_tag="ACHVC7_003401"
     CDS             4453..5775
                     /gene="mepM"
                     /locus_tag="ACHVC7_003401"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR2117368.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHVC7_003402"
                     /gene_synonym="msbB"
     CDS             5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHVC7_003402"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR2117369.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6954..8396)
                     /locus_tag="ACHVC7_003403"
                     /pseudo
     CDS             complement(6954..8396)
                     /locus_tag="ACHVC7_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2254 a         1982 c         2085 g         2075 t
ORIGIN      
        1 ttttcagttc atcgagacac ctcgcaagtt ttcttcatcc ttcgctggat atctatccag
       61 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
      121 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
      181 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
      241 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
      301 aacaataaac aagagcgcac gttgtttaaa gagttaatca aaaccaacgg cgtcggcccg
      361 aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgaa
      421 cgtgaagaag tgggagcact ggtgaaactg ccgggcattg gtaaaaaaac cgccgagcgt
      481 ttaattgtcg aaatgaaaga ccgatttaaa ggtttgcacg gcgatctctt tacgccagcc
      541 gccgatctgg tgctcacgtc gcctgccagc ccggcaaccg acgatgctga acaggaagcg
      601 gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat ggtgagcaaa
      661 atcgctcgca ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
      721 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
      781 atggggcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
      841 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
      901 atctgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
      961 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatc
     1021 tggcagcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat gagatccacc
     1081 gtctatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac caactggata
     1141 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     1201 tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac cgttttggta
     1261 ttgtgcaacg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg
     1321 cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct cgtcgcgctc
     1381 gcggtacgcc acgtattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     1441 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gctggatatg ttgaatgtcg
     1501 atgctgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc gataagttct
     1561 ttggtgggcc ggtaggtctg gataacctgg cggcagccat tggcgaagaa cgtgaaacca
     1621 ttgaggatgt gctggagcct tatctgattc agcaaggctt tttgcagcgt acaccgcgtg
     1681 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     1741 aagtcggatt ggtcgctcgc accgcatccg acattggcag tgtgcctgat gcgacgctat
     1801 tcgcgtctta tccggcctac cagagatagc gaaccgtagg tcggataagg cgctcgcgcc
     1861 gcatccgaca aatgtgttca gcgacagatt agctggcctg ctttttcatc atactgataa
     1921 taaataacag tgcagcacat agcactaccg acggacccgc cggcgtatcg taaaacgcgg
     1981 aaaaggttaa accgccagtt actgccacca tccccaccaa aacagcgaca ccagccatct
     2041 gttccggcgt acgggcaaag cgacgcgcag tagctgcagg aataatcagc aacgaagtaa
     2101 taatcaacgc accgacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca
     2161 acaacaattt cacgcgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg
     2221 tcatcgacag caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa
     2281 tcgcaataga gatgagatct tctggcgtca ctgccagcaa atcaccgaac aggtaagcca
     2341 tcaaatcaac acgaatatta gacatcagac taacgaccac caggcccagc gacagggcac
     2401 tgtgcgccat aatccctaat aacgtgtcga tcgccagctg tggacgcttc tccagccata
     2461 ccagaccgcc cgccagcagc agcgtaacgg caatcaccgc atagaatgga ttcacgtcca
     2521 gcaacaaacc aaacgcgaca ccaagtaatg aggcatgagc cagcgtatca ccgaaataag
     2581 acatacgacg ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc
     2641 cggctaacca accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac
     2701 aattcgtccc tgtaaatcgt gacgatggtt atgatgatgg cgatagatac ccagttgttc
     2761 agcaccacga ggaccaaaca ttgaaataaa ctccggatgc agggaaacaa cttccggtgt
     2821 gccggaacaa caaatgtggt gattcaggca cagcacttca tcggtttttg ccatcaccag
     2881 atgcagatcg tgagaaacca ttaaaacgcc acaatccagt tcacgacgca gttggtcaat
     2941 aaggtcatat aacgccacct ggccatttac atccacgcct tgagtgggtt catccagcac
     3001 taataattgc gggcgattta acaatgctcg cgctaacagt acacgctgcg tttcgccacc
     3061 agagagcttt tgcatcggtg cgttaatcag gtgcccggcc tggacacgtt tcagtgcagg
     3121 caaaatatct tctttatgtg taccagggcg taagcgtaaa aaacggttta cggtcagtgg
     3181 caacgtggtg tcgagataca gcttctgcgg tacatagccg atgcgcagtt ttccgttgcg
     3241 cttgataacc ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt
     3301 acctgcgcca tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac
     3361 atcagagagg acgcggcgtt ggccaaaaga aaccgagaca ttttccaagg aaaccagact
     3421 tgtcatgtta attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata
     3481 ttcattacga tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta
     3541 tccgccgctc tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt
     3601 aaacccgttg ggttcatcgc ttctgccatt gctgatgggg taacagaaac agaggtttta
     3661 cttcctgacg gcgcttcaga acatgattat tcactgcgcc catcggatgt aaaacgctta
     3721 cagaacgcgg acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg
     3781 gtaagcaaat taccaggagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg
     3841 ctactgatga aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac
     3901 gaagatcacc atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg
     3961 gctacagcgg ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa
     4021 cttgacgcca acctgaagga ttttgaggca caattagcct caaccgaaac gcaggttggt
     4081 aacgagctcg cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat
     4141 ttcgaaaaac agttcggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa
     4201 cctggcgcgc agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc
     4261 gtttttgctg agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc
     4321 gttcgtatgg gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat
     4381 tcagaattcc tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga
     4441 ggaagtgatt acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg
     4501 cgaccacacc gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg
     4561 cggccctatg tttatcaccg cgatgccacg ccaattgtca aaaccattga gctggaacag
     4621 aacgaaattc gttcgctctt acctgaagcc agtgagccga ttgatcaagc tgcacaagaa
     4681 gatgaagcca ttccccagga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat
     4741 gaatatgttg tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat
     4801 atgggtgata tcacccaact ggctgcggcc gacaaagaat tgcgtaacct gaaaatcggt
     4861 caacaactct cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg
     4921 tctcgtcgtg aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc
     4981 gaaatgcagc aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg ggggagcttt
     5041 gttgccagcg ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc
     5101 atgcagtggc aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtgttaatg
     5161 tctcgagaaa tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt
     5221 tcagaaggta aagattatta cgctatccgc gctgaagatg gcaaattcta cgatcgtaac
     5281 ggtactggtc tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg
     5341 tctaacttta acccgcgtcg tactaatccg gtgaccggtc gcgttgcagc acacagaggt
     5401 gttgatttcg ccatgccgca gggtacgcca gtgctttcag tgggtgacgg tgaagtggtg
     5461 gttgccaaac gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac
     5521 accacgcgtt atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt
     5581 ggcgaccgta tcgcgctttc cggtaatacc ggacgttcaa ccgggccgca tctgcactat
     5641 gaagtatgga taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtaccgaa
     5701 gggctgaccg gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag
     5761 ctacggtttg attaattaac atccattcgc agccggtacg cagtcagtat cggctttttt
     5821 tatttggtgc ggggcaagtt gcgccgctac actatcacca gattgatttt tgccttatcc
     5881 gaaactggaa aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata
     5941 aatcctttcg ccacccgcgc tactggggag catggctggg tgtagcagcg atggcgggta
     6001 tcgctttaac gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg
     6061 gacgactggg aaaaagctca cgccgtcgtg cgttaatcaa tctgtcgctc tgctttccag
     6121 aacgtagtga agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag
     6181 cgatggcaat gatggctgag ttggcaatac gcggaccgga gaaaattcag ccgcgcgttg
     6241 actggcaagg gctggagatc atcgaagaga tgaggcgtaa taacgagaaa gttatctttc
     6301 tggtgccgca cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga
     6361 aaatggcagc gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg
     6421 tgcgtcgtcg ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc
     6481 agtcggtacg tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc
     6541 acagcgaatt tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt
     6601 tgatgaaagt gtgccgtgcg cgcgttgttc cgctgtttcc gatttatgat ggcaagacgc
     6661 accgtctgac gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata
     6721 cgattgcgcg gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat
     6781 acacctggat attaaaattg ctgaaaactc gcaaaccggg cgaaatccaa ccgtataagc
     6841 gtaaagatct ttatcctatc aaataaaaaa agcctctcaa attattgaga ggctgtactc
     6901 gataaaaaat ctcaatgagt gttcccacta ttttgagttt ttaggttagt acgctattga
     6961 gttaatcgta gcgtgataat ttcaaaaggt ttgaactcaa gattgcaata ttgattgcaa
     7021 taaaaactgt catctaactt attttcttga agatcgacat attgaacgta gtgataatta
     7081 catactcacg aaagcatggc tgaaatttct aatccatgac tctcatataa tcttacgatt
     7141 ataccgtcgc cgtcttcagc taattttatt gtttcgatga taacgctgtt agtatcgata
     7201 gcaaaaaatg atagtgaagg gggtaattca cccgggtgcg gtttgacagg gacgacctgt
     7261 aagggatgat tcattatata ggcttgatta ataacaccac ccacacggta atctccatga
     7321 tgaggaaata aggagaatat gaatttttgt ttaccaatat ctgcaaaatt attaatcagg
     7381 aaattatcat aattgtctgt cttaacaact tccccaggtt tatgctgcga acgcagcaaa
     7441 gttaattcaa gcgtctggtc tttgacacga tagccatatt tgttttttgc tattaaagct
     7501 atgccagttt ctcgatcaga aaggtcgacc cagtgatgtg ctggtatttc atctttggca
     7561 aaagcgaacg aatcatcgct atgcgttggt ctgtcaatta caccgaactg aatttgacat
     7621 tgtgcaagag atgtttgtac tgacaccggg aattttactt tgagagaaac attaggttcg
     7681 tgccaatcaa taaacgtttg gacatcaacg cgtggactat cctgtgtcag ggtaaaggct
     7741 aaacgaagat aagaggaatt gtagtgataa atcagcgtca tcgtagtttt aggcccgctg
     7801 atcacagttg tcattttttc agcgagtaaa acatatgatt ccccatcacg ataatttgaa
     7861 gcaaaatccc aggccgcgaa aaaacctgca tcttcgtggt atgcacggat ctcactgtgc
     7921 atattttcag taatgtattc tttaccatat cttttatcat atagcgaaat taaatcaccg
     7981 ctttctgaaa aaagaagttt aatgtaatta ttttcaatac tttgagattc tgccttcaga
     8041 ccatttacga tcttattctt tggaacaatc acagcataac cataagagtt tactcgtgct
     8101 ttgagccagt tgttgtttat attgatccat tcgttacgag caaaacaggt ggtattaaac
     8161 aaaatgtgcg gatctttata agaagaagtg tcaattttac ttaataaagt agactgcgca
     8221 tcagaaataa ttttctcggt ttttgcttct agtttaagat attccttttc agtttcatga
     8281 taaactcttg aaatacatga gccgggaagg atgtcatgga attgcaaggt cagcatcgct
     8341 ttccatattt catcaaactc actgcgaaga attgatgtca tattattagt aatagt
//
LOCUS       DAZPLM010000181         8372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPLM010000181 DAZPLM010000000
VERSION     DAZPLM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.181"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(109..185)
                     /locus_tag="ACHVC7_003404"
     tRNA            complement(109..185)
                     /locus_tag="ACHVC7_003404"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Asp,seq:gtc)
     gene            complement(318..1049)
                     /gene="dnaQ"
                     /locus_tag="ACHVC7_003405"
     CDS             complement(318..1049)
                     /gene="dnaQ"
                     /locus_tag="ACHVC7_003405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR2117370.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1114..1581
                     /gene="rnhA"
                     /locus_tag="ACHVC7_003406"
     CDS             1114..1581
                     /gene="rnhA"
                     /locus_tag="ACHVC7_003406"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR2117371.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1578..2300)
                     /locus_tag="ACHVC7_003407"
     CDS             complement(1578..2300)
                     /locus_tag="ACHVC7_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2117372.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2334..3089
                     /gene="gloB"
                     /locus_tag="ACHVC7_003408"
     CDS             2334..3089
                     /gene="gloB"
                     /locus_tag="ACHVC7_003408"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR2117373.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3161..4519
                     /gene="mltD"
                     /locus_tag="ACHVC7_003409"
     CDS             3161..4519
                     /gene="mltD"
                     /locus_tag="ACHVC7_003409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR2117374.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4568..5191)
                     /locus_tag="ACHVC7_003410"
     CDS             complement(4568..5191)
                     /locus_tag="ACHVC7_003410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2117375.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5195..5995)
                     /locus_tag="ACHVC7_003411"
     CDS             complement(5195..5995)
                     /locus_tag="ACHVC7_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR2117376.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6236..7150
                     /gene="yafC"
                     /locus_tag="ACHVC7_003412"
     CDS             6236..7150
                     /gene="yafC"
                     /locus_tag="ACHVC7_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR2117377.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7147..7950)
                     /gene="dkgB"
                     /locus_tag="ACHVC7_003413"
     CDS             complement(7147..7950)
                     /gene="dkgB"
                     /locus_tag="ACHVC7_003413"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR2117378.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8113..8189)
                     /locus_tag="ACHVC7_003414"
     tRNA            complement(8113..8189)
                     /locus_tag="ACHVC7_003414"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8153..8155),aa:Asp,seq:gtc)
BASE COUNT         2233 a         2020 c         2024 g         2095 t
ORIGIN      
        1 ttacagcata acaaaaccat agaccgaaga aatagcggga tttaaatgca aaaaaaccgc
       61 aaaattaaaa atcttgcggt tctctgaact cattttcatg agtgaatttg gcggaacgga
      121 cgggactcga acccgcgccc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
      181 gctccgcgtt gtgttccgtt gggaacgagg cgaatattac ggattgcctc acccttcgtc
      241 aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat cgcccaagcc gctattttta
      301 gcgcctttta caggtattta tgcccgccag aggcagcttc cgcccttctt ctccaccaga
      361 tcaagacggg cttcatgagc tgcaagctct tcatctgtcg caaaaacaac gcgtaactta
      421 cttgcctgac gtacaatgcg ctgaattgtt gcttcacctt gttgctgctg tgtctctcct
      481 tccatcgcaa aagccatcga cgtttgacca ccggtcatcg ccagataaac ttccgcaagg
      541 atctgtgcat cgagtaatgc cccgtgtagc gttcgtttac tgttatctat ttcgtagcgg
      601 gcacataacg catcgaggct gttgcgctta ccaggaaaca ttttcctcgc caccgcaagg
      661 ctatcggtga ccttacagaa ggtattggtc ttcggaatat cgcgcttaag caacgaaaac
      721 tcgtagtcca taaagccgat atcgaacgct gcgttatgga tcaccaactc cgcgccgcgg
      781 atatagtcca tgaactcatc ggctacttcg gcaaacgtgg gcttatcgag caaaaattca
      841 tcggcaatac catgtacgcc aaaggcttcc ggatccacca gccgatcggg tttgagataa
      901 acatggaagt tattgcccgt caggcgacgg ttcaccactt caacggcacc aatctcaatg
      961 atcttgtggc cttcatagtg cgcaccaatc tggttcatac cggtggtttc ggtatcgaga
     1021 acgatctggc gtgtaattgc agtgctcata gcggtcattt atgtcagact tatcgtttta
     1081 cagttcgatt caattacagg aagtctacca gagatgctta aacaggtaga aattttcacc
     1141 gatggttcgt gtctgggcaa tccaggacct gggggttacg gcgctatttt acgctatcgc
     1201 ggacgcgaga aaacctttag cgcaggctac acccgcacca ccaacaaccg tatggagttg
     1261 atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt gcgaagtcat tttgagtacc
     1321 gacagccagt atgtccgcca gggtatcacc cagtggatcc ataactggaa aaaacgtggc
     1381 tggaaaaccg cagacaaaaa accagtaaaa aatgtcgatc tctggcaacg tcttgatgct
     1441 gccctagggc aacatcaaat caaatgggaa tgggttaaag gccatgccgg acacccggaa
     1501 aacgaacgct gtgatgaact ggctcgtgct gcggcaatga atcccacact ggaagatacg
     1561 ggctaccaag tcgaagttta agcttgtggt ttacgacatt gccgggtggc tccaaccgcc
     1621 tggcgaattc gtggcttgtt tttactctgt ttcatcgggt ttagcgttaa aggaatagtc
     1681 cgtttccggg caacaataag ttgtaagcaa ccaagcgcag gaatatgcgc attcaatagt
     1741 tttcctccgt gtttgttcca cgggagaacg tggaaacggc tggcgtgcag cacttcaaaa
     1801 ttcaacaaag agagccagtc cagttgccgc atcagagtaa acatccggct gttatagggc
     1861 gaggtttttc gcaataccgg cacaagtttg cgtaatccca ttaaactaat gggattgaag
     1921 ccactaatca ccagccagcc atcatcaatc aatacccgat cggtttcacg caataaacga
     1981 tgcggatcgg tgcaccacgg caatgtatgt gccagtagac aaacatcaac ggatttatcg
     2041 gcaaaaggaa gatgaagtgg atccgcctgc acctggacgg gcattccttg cgcagaaaca
     2101 ttcacttgat gagaaaccgc gcacgcttcg caattgattt ctgcgcttaa attgccaatc
     2161 ttaagcagat gaaaaccata cattttagtg aaccacgggt tgagctggcg ctccagcgcc
     2221 ttgcgataaa gctctcccca gggcaaatcg ccccagcgat caggagccac gacagtttga
     2281 gggactcttg ccggtttcat cacaaccttc cgtttcacac taagaggtaa tctatgaatc
     2341 ttaacagtat tcccgccttt gatgacaatt acatctgggt tttgaatgat gaagcaggtc
     2401 gctgcctgat tgtcgatccc ggagacgcag agccagtatt aaacgccatt gccgccaata
     2461 actggcaacc ggaggccata tttctcaccc accatcatca cgatcacgtt ggcggcgtaa
     2521 aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg tccacaagag acacaagata
     2581 agggaacaac acaggtagtc aaagatggcg aaactgcctt cgttttgggg catgaattta
     2641 gtgtaattgc cacaccgggt cacactttag gacatatctg ttacttcagt aaaccttatc
     2701 tattttgcgg cgacacgctg ttttctggtg ggtgtggtag gttgtttgaa gggacagcat
     2761 cacaaatgta tcaatcactt aataagttaa gtgcgttacc tgacgataca ttggtatgtt
     2821 gtgctcatga atatacgtta tcaaatatga agtttgcttt gagtattctt ccgcacgatt
     2881 tgtccataaa tgattattat cgtaaagtta aggagttacg ggcaaaaaat caaataacac
     2941 tacccgtaat tctgaaaaat gagcggcaaa ttaatgtttt tttaagaacg gaagatattg
     3001 atttaattaa tgtaattaat gaagaaacat tattgcaaca acctgaagag cgttttgcat
     3061 ggttaaggtc aaagaaagat aggttctgat aaaactttct tgtcatcggc tccgttcgcc
     3121 gttatgatcg gtcgtctttt aagcaactat tgacacacac atgaaggcaa aagcgatatt
     3181 actcgcctct gtcctgctcg tgggttgcca gagtaccggc aacgttcaac agcacgcaca
     3241 gagcctttct gcagctggtc aaggggaagc agcaaagttt acaagtcagg cacgatggat
     3301 ggacgatggg acgtctatcg cgccagatgg tgacttgtgg gctttcattg gcgacgagct
     3361 aaagatggga attccggaaa atgaccggat tcgcgaacag aaacagaaat atttacgcaa
     3421 taagagctat ctccacgatg taactttacg ggcagagccg tatatgtact ggatagcagg
     3481 gcaagttaaa aaacgtaaca tgcctatgga actggtacta ctacccatag tggagagcgc
     3541 ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc atctggcaga tcattccgag
     3601 cacggggcgc aattatggtt tgaaacagac ccgcaattat gacgcgcgtc gcgatgttgt
     3661 tgcttcaaca actgccgcgc tgaatatgat gcagcgtctg aacaaaatgt ttgacggcga
     3721 ctggcttctg accgtagcgg cttataacag cggcgaaggt cgggtcatga aggcaattaa
     3781 aacgaacaaa gcgcgtggga aatccacgga cttctggtcg ttaccgttgc cgcaggaaac
     3841 gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt ctcaaaaaca gcaagcgtta
     3901 tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg gcgcgtgtgc acctgagtag
     3961 cccggttgaa atggcgaagg ttgcagatat ggcggggatt tccgtcagca agctgaagac
     4021 attcaacgct ggcgtgaaag gctccacgct gggcgcaagt gggccgcagt acgtgatggt
     4081 gccaaagaag catgcagatc aactgcgtga atctctcgct tcaggcgaaa ttgctgctgt
     4141 acagtcgacg ctggttgccg acaatacgcc gcttaacagc cgtgtttaca ccgtacgctc
     4201 tggcgacacg ctttcaagca tcgcttcacg tctcggcgta agcaccaaag atttgcagca
     4261 gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa agtttgacga ttggtgcagg
     4321 cagtagcgca cagcggttgg caaacaacag cgatagcatt acgtatcgtg tgcgcaaagg
     4381 cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac atcaaagatg tgatgcgctg
     4441 gaacagcgac actgcgaatc tgcaaccagg cgataagctg acgttgtttg tgaaaaacaa
     4501 caacatgcca gattcctgac aaaccagata ataaaaaagg caccgattcc cccggtgcct
     4561 tttttattta tgccgcttta tgtgcttcta ccatgatgat atcactggtg aaagagccat
     4621 cagtctgcaa ggaaaaatac gttctcacct ctgtcgatgc gctctgttgg taaatgcgaa
     4681 tagcgtctac taacgcttct ggcgtacgca ttctcacgac ccatgaagaa aattccagcg
     4741 gtaacttatc tgtaattaaa ttatcaacta tcagattggc ttcattgatt aacgtcaacc
     4801 acgcaccgct ggcgtagttt cgtacgtgag aggtatcgcg taatgcttct accgcctgta
     4861 accagatgtc gcgcactggg tgacccggag acattacatc catcacaatc agcctaccgc
     4921 caggtttcaa tatcctattc acttctcgca gtgctgcacc aacatcatgc cagtgatggg
     4981 cagaataacg gctgataaca atatcaaatg cgttatcggc aaatggcaga cttgcggcat
     5041 atccctggcg ggtggtgata tttttcagtt gccgggcttc ggcagcttgt gccacgacat
     5101 ccagcatgtg ggcagataag tcatacgcca ccaccgcgct cacgttttgc gcagcgccaa
     5161 acgttggtct gccctgcggc agacctgaca taccttattt atcaggcttg ccgggactga
     5221 attcaacgag tagcggattg tgatcggaag cgcgcgtaac cagtacagaa gcttcactga
     5281 cgttcagacc acggtagaaa acaaaatcga gcgggcgacc aaacgcccga cggcgctgat
     5341 catcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat aacgcgttca
     5401 tccttctacg gctccaggca ttgaaatctc ccgccataat gaccgggccg ctgtggtgag
     5461 ctatctgatc accaatagga agtaactgct tactatagac atccacgccc agactgaagt
     5521 tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga tagaccgtca
     5581 ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg cagcaataca
     5641 ctggatgtgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagc acgaaagcgg
     5701 gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg taccaactct ggcgttgttt
     5761 gcgcttcctg caataacacc agatgtgcgt ctttgccgta attctttaat accgacaacc
     5821 attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc tcttcggtac
     5881 ttaacggttc tccgggtggt aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga
     5941 tcctctccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt cgcacttttg
     6001 actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac tgttataggg
     6061 attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag tgcttacacc
     6121 ggtcagaaat ttgctcatta ttgagaacat taaatcggat gacatatcac gcatcctgat
     6181 ggtttaatga caaataaatt caaaatatct catctatttt gcaacaggag caaaaatgaa
     6241 agccacgtcg gaagaactcg ccatttttgt ttcggtcgtc gaaagcggca gctttagccg
     6301 ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcggtga aaaagctgga
     6361 aatgaaactt ggcgttagcc tgcttaatcg gaccacgcgg caacttagcc tgacggaaga
     6421 aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcag cggcagaatc
     6481 agaaattatg gagacgcgta atacaccgcg tggactgtta cggatcgatg ccgcaactcc
     6541 agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct atccggaagt
     6601 cactttatcg ctagtctcct ccgaaacgat tattaatttg atcgaaagaa aagtggatgt
     6661 cgcgatacgc gctggtacgt taacggactc cagcttacgt gccaggccgt tatttaacag
     6721 ttatcgaaaa attatcgcct cccccgatta tatttcccgc tacgggaagc cagaaacgat
     6781 cgacgattta aagcaacatg tttgcctggg attcactgaa cccgcttccc tcaatacctg
     6841 gccgatagcc tgtagtgatg gacaattaca tgaggtgaag tacggtttgt catccaatag
     6901 tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt tgtccgacta
     6961 catgatcgac aaagaaatcg ctcgcggaga attggtggag ttaatggcag ataaagtgtt
     7021 gccagtggaa atgccattca gtgccgtcta ttacagcgac cgtgcggtaa gtacgcgcat
     7081 ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag gagctgtcag
     7141 agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt cgttgcaatc
     7201 cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg ccttaagatt
     7261 actttccagg tttttacgtt tagtagaaga aggaattact gagtaacctt cccccatagc
     7321 ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcaatac gagcaataac
     7381 ctcatctttc agtgccttac cataagccag cgtcatatag gaagtgatat gaataccgtg
     7441 ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt caatctggtt
     7501 ggtagcgatg ttttcagcgc caacagcagc aatcgctttt tccatcaacg ggatcgtgaa
     7561 gttggaaata ccgatctcac gtgtcagacc ttgttttttg gcctccagca gcgcctgcat
     7621 aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg tcagatcaac
     7681 ataatcggta cgcaattttt gcaggctctc ttttagactc gggatcaatt tgtctttgct
     7741 gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc
     7801 tgcaatcgcc tgacctactg cggcttcgtt atcatagatt tgtgcagtat caattgcgcg
     7861 ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt tgagacggaa
     7921 agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta
     7981 actgaggtga ttatgggctt cagactgttg cggaaaaaga gcataaaatg cagaggattt
     8041 ttgcgattct ggcaataata gatatacaga cgaaaaaaaa cctcgagcgt taactcaagg
     8101 cttcttaata agtggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
     8161 ggtattctaa ccgactgaac taccgctcca ccgaattctt ttacaaccac cggttttatg
     8221 accggcttac tgcttaattt gatgcctggc agtttatgaa tcacttcgtg attcaccctt
     8281 cgggccgttg cttcgcaacg ttcaaaagct ttcgcttttg tcctactctc ttatcgagta
     8341 gtgaactgcc tcaaaggtca gtgccattct tc
//
LOCUS       DAZPLM010000182         8231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPLM010000182 DAZPLM010000000
VERSION     DAZPLM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.182"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            10..732
                     /gene="sdiA"
                     /locus_tag="ACHVC7_003415"
     CDS             10..732
                     /gene="sdiA"
                     /locus_tag="ACHVC7_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR2117379.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(799..1023)
                     /gene="yecF"
                     /locus_tag="ACHVC7_003416"
     CDS             complement(799..1023)
                     /gene="yecF"
                     /locus_tag="ACHVC7_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR2117380.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1010..1186)
                     /gene="yecU"
                     /locus_tag="ACHVC7_003417"
     CDS             complement(1010..1186)
                     /gene="yecU"
                     /locus_tag="ACHVC7_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR2117381.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            1482..2138
                     /gene="uvrY"
                     /locus_tag="ACHVC7_003418"
     CDS             1482..2138
                     /gene="uvrY"
                     /locus_tag="ACHVC7_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR2117382.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            2135..3967
                     /gene="uvrC"
                     /locus_tag="ACHVC7_003419"
     CDS             2135..3967
                     /gene="uvrC"
                     /locus_tag="ACHVC7_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR2117383.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            4024..4572
                     /gene="pgsA"
                     /locus_tag="ACHVC7_003420"
     CDS             4024..4572
                     /gene="pgsA"
                     /locus_tag="ACHVC7_003420"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR2117384.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            4724..4799
                     /locus_tag="ACHVC7_003421"
     tRNA            4724..4799
                     /locus_tag="ACHVC7_003421"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4757..4759,aa:Gly,seq:gcc)
     gene            4854..4927
                     /locus_tag="ACHVC7_003422"
     tRNA            4854..4927
                     /locus_tag="ACHVC7_003422"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4886..4888,aa:Cys,seq:gca)
     gene            4940..5026
                     /locus_tag="ACHVC7_003423"
     tRNA            4940..5026
                     /locus_tag="ACHVC7_003423"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4974..4976,aa:Leu,seq:taa)
     gene            5221..5886
                     /gene="yecA"
                     /locus_tag="ACHVC7_003424"
     CDS             5221..5886
                     /gene="yecA"
                     /locus_tag="ACHVC7_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR2117385.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5948..7159)
                     /gene="tyrP"
                     /locus_tag="ACHVC7_003425"
     CDS             complement(5948..7159)
                     /gene="tyrP"
                     /locus_tag="ACHVC7_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR2117386.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            7350..7589
                     /locus_tag="ACHVC7_003426"
     CDS             7350..7589
                     /locus_tag="ACHVC7_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR2117387.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            complement(7627..8124)
                     /gene="ftnA"
                     /locus_tag="ACHVC7_003427"
     CDS             complement(7627..8124)
                     /gene="ftnA"
                     /locus_tag="ACHVC7_003427"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR2117388.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
BASE COUNT         2170 a         1919 c         2091 g         2051 t
ORIGIN      
        1 cggtttacta tgcaggatac ggattttttc agctggcgtc gcacgatgct gttgcgtttt
       61 cagaggatgg aggccgcaga agaggtctac catgaaatag agcttcaggc tcagcagctg
      121 gagtacgatt actattcgtt atgtgttcgc cacccggtac cattcacgcg acctaaagtg
      181 gctttctaca ccaattaccc tgagtcgtgg gttagttatt atcaggcaaa aaattttctc
      241 gcaattgatc ctgtgctgaa ccctgaaaac tttagtcagg gccatttaat gtggaatgat
      301 gacttattca gcgaagcaca gccattatgg gaagccgcgc acgcacatgg tttacgccgc
      361 ggtgtcactc agtatttaat gctgccaaat cgggcgctgg gctttttgtc cttttcccgt
      421 tgcagcacgc gcgaaatacc cattcttagt gatgaactgc aattaaaaat gcagttactg
      481 gtgcgcgaaa gtctgatggc tctgatgcgt ttaaatgatg aaatagtgat gacgccagag
      541 atgaatttca gcaagcgcga aaaagaaatt ctgaaatgga cggcggaagg gaagacatca
      601 gcagagatag cgatgatttt gtcaatctct gagaatacgg tcaatttcca tcagaaaaac
      661 atgcagaaaa aaatcaatgc accaaataag acccaggtgg cctgttacgc ggccgctact
      721 ggcttaattt gatcgctttt ctttctgcgt gccagatgca aaaatcggct gaaaggcacg
      781 ctctcagccg gttttatatt actgacggta ggcttgttta atttgcttaa ccgttttgga
      841 aaatactgca gcctgggttt cgtcttcgat cagcgcaagc tgtttttcca tctttaacat
      901 cacgcggccc gcgtcagctt gtcccatcgc ctgtagcatc agcgtcagca tcgctttcag
      961 gcaggagact tcatttgcca gttcttgatt attctcggca gtagaaaaat caggcgtact
     1021 catttatttt cctcgtcatg ttgcaatgaa aatttgcggt gaaaaatgtt aaggcggcgg
     1081 agtataccat aagctttgct aaaaatagca gtggttgttt tttgagcgtg acgtcaacaa
     1141 tgctattaaa tgcgttaata taatgaccaa taaatatttt tatcatgaat gttttttgcc
     1201 cgtattgttc gggttaatta atgttacata ttcagcgggc tgattttcat ttttgctgaa
     1261 taaagtcaat ttttgtcaca tttcatcgcc gggcttactg tgaaacgatc ccggaagccg
     1321 ttggtgacgg gcgtgaccat aactgtggac aatcgaattt acaaaaacga gagaaaaatc
     1381 gaatacccac catttttaac gtttcaaagt tgcaataaaa accgctaata tacgaatgac
     1441 taactatcag tagcgttatc cctatttctg gagatattcc tttgatcaac gttctacttg
     1501 ttgatgacca cgaactggtg cgcgcaggga tacgacgcat tctggaagat ataaagggta
     1561 taaaagtcgt cggtgaggca tcgtgcggtg aagacgccgt taagtggtgc cggacaaatg
     1621 ccgttgacgt ggtgctaatg gacatgagta tgccgggcat tggcggtctt gaggcgacgc
     1681 gtaaaatcgc gcgttccaca gctgatgtca aaatcatcat gcttaccgtc catacagaaa
     1741 accctttacc agcgaaagtc atgcaggccg gtgctgcggg ctacctcagc aaaggcgcgg
     1801 ctccgcagga agtcgtgagt gcgattcgtt ctgtctattc agggcagcgt tacattgctt
     1861 ctgatatcgc tcaacaaatg gcgttaagcc agatcgaacc agaaaaaaca gaaagcccat
     1921 ttgccagttt gtctgaacgt gaattgcaga ttatgctgat gatcaccaag ggccagaagg
     1981 tcaatgagat ctcagaacag ctcaatctca gtccgaaaac ggtgaacagc taccgctatc
     2041 gtatgttcag taaactaaac attcatggcg atgttgagct gactcacctg gcaattcgcc
     2101 atggtctgtg taatgcggag acattatcaa gtcagtgagt gatcagtttg acgcaaaagc
     2161 gtttttaaaa accgtaacca gccagccagg cgtttatcgc atgtacgatg ctggtggtac
     2221 ggttatctat gtcggcaaag cgaaagacct gaaaaaacgg ctttccagct atttccgcag
     2281 caacctcgct tcgcgcaaaa ccgaagcgct ggtcgcccag atccagcaaa ttgatgtaac
     2341 ggttacgcat acagaaaccg aagcgctgtt gctggaacac aactacatca aactctatca
     2401 gccgcgttac aacgttttgc tacgcgatga taaatcttat ccttttatct tcctgagtgg
     2461 cgatacccac ccgcgtctgg cgatgcatcg tggtgcgaag catgccaaag gtgaatattt
     2521 cggcccgttc ccgaatggct atgccgtacg tgaaacactg gcgctactgc aaaagatttt
     2581 ccccattcgc cagtgcgaaa atagtgttta tcgcaatcgc tcgcgtccgt gtctgcaata
     2641 tcagataggg cgctgtctgg gaccgtgcgt tgaaggactg gtgagtgaag aagaatacgc
     2701 tcagcaggtt gagtatgtgc gcctgttttt gtctggcaaa gatgatcagg tgcttacgca
     2761 actcattagt cgtatggaaa ctgccagcca gaatctggag tttgaagaag ctgcacgtat
     2821 tcgcgaccaa attcaggcgg tgcgacgcgt caccgaaaaa caattcgttt ccaataccgg
     2881 cgacgacctc gacgttattg gtgtggcgtt cgatgcgggc atggcttgtg tccacgtatt
     2941 gttcattcgt cagggcaaag tgctcggcag ccgcagctat ttcccgaaag tgcctggcgg
     3001 tacggaactg agcgaggtgg tagaaacctt cgtaggccag ttctatttac aaggcagcca
     3061 gatgcgcacc ttaccgggtg agatcctgct cgattttaat cttagcgata aaacgctgct
     3121 cgccgattcc ctttcagaac tggcgggacg caagattaat gttcaaacca aacctcgcgg
     3181 cgatagggcg cgttatctga aactcgcgcg caccaatgcg gcgacggcct taaccagcaa
     3241 actttcgcag caatctaccg ttcaccagcg actgaccgcg cttgccagcg tgttgaaatt
     3301 gccggaagtg aagcggatgg agtgctttga catcagccat accatgggcg aacaaaccgt
     3361 cgcttcctgt gtggtgtttg atgctaacgg cccgctgcgt gcggagtatc ggcgctataa
     3421 cattacaggc atcacgccgg gcgatgatta tgcggcgatg aatcaggtgc tgcgtcggcg
     3481 ttatggtaaa gccattgacg acagtaagat cccggatgtg atccttatcg acggcggcaa
     3541 aggccagctt gcgcaggcga aaaatgtctt cgccgaactg gatgtctcat gggataaaaa
     3601 tcatccgctg ctacttggcg ttgccaaagg agcagatcgt aaggctgggc tggaaacgct
     3661 gttctttgag ccggaaggtg agggattcag tttgccgcca gattctcccg cgctgcatgt
     3721 tatccagcat attcgcgatg aatcacatga tcacgcgatt ggcgggcacc gtaaaaaacg
     3781 ggcgaaggtc aaaaatacca gttccctgga aaccattgaa ggcgtcgggc caaaacgtcg
     3841 gcaaatgttg ttgaaatata tgggcggttt gcaaggttta cgtaacgcca gcgtcgagga
     3901 aattgcaaaa gtgccgggta tttcgcaagg tctggcagaa aagatcttct ggtcgttgaa
     3961 acattgatgt ctctgtagca acataggggt aatcttactg acaacagata gttacccgtc
     4021 attatgcaat ttaatatccc tacgttgctt acactgttcc gtgtcatcct tatcccattc
     4081 tttgtattgg tcttttatct gcctgtcacc tggtcgccgt ttgccgccgc gctcattttc
     4141 tgcgtcgcgg cggtgactga ctggttcgat ggttttctgg cacgccgctg gaaccagagt
     4201 acccggtttg gtgctttcct tgaccctgta gcagataaag ttctcgtggc tatcgccatg
     4261 gtgctggtaa ccgagcatta ccacagctgg tgggtgacct taccggcagc aacgatgatc
     4321 gcccgtgaaa ttattatttc tgcgctacgc gaatggatgg cggagttggg taaacgcagt
     4381 agcgtggctg tctcctggat tgggaaagtg aaaaccactg cccagatggt ggcgttggca
     4441 tggctgctgt ggcgtccgaa catttgggtt gagtacgccg gtattgcact tttctttgtg
     4501 gctgcggtac tgactctgtg gtcaatgttg caatatttga gcgctgcgcg tgcagatttg
     4561 cttgatcagt gatcgtttcg gcgtaatttt cagcaaacga tcaaaagtgg tgaaaaatat
     4621 cgttgactca tcgcgccagg taagtagaat gcaacgcatc gaacggcggc actgattgcc
     4681 agacgataat aaaatcaagt gattaactga ttgcttgatg aatgcgggaa tagctcagtt
     4741 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccag
     4801 tttaaaagac atcggcgtca agcggatgtc tggctgaaag gcctgaagaa tttggcgcgt
     4861 taacaaagcg gttatgtagc ggattgcaaa tccgtctagt ccggttcgac tccggaacgc
     4921 gcctccactt tcttcccgag cccggatggt ggaatcggta gacacaaggg atttaaaatc
     4981 cctcggcgtt cgcgctgtgc gggttcaagt cccgctccgg gtaccatggg aaagataaga
     5041 ataaaatcaa agcaataagc agtgtcgtga aaccaccttc gggtggtttt ttgtgcctgc
     5101 aacttgtcgt tacaccctcc ttaattttta atcaccggca aagccactca aaaagtatct
     5161 ctgatacgga ccggcatgta agataggtgc tggcgagttg agatccacaa ggaaaagcgt
     5221 atgaaaacgg gaccgttaaa cgaaagtgag ttggaatggc tggacgatat tctgaccaaa
     5281 tacaacacta accacgccat ccttgatgtg gcggagctgg acggtttatt gacggcggtg
     5341 ttgagttctc cgcaagagat tgaaccggaa cagtggctgg ttgccgtgtg gggtggggct
     5401 gactatgtgc cgcgctgggc gtcagagaaa gagatgacgc gctttatgaa cctggctttt
     5461 caacatatgg ccgataccgc agagcgtctg aacgaattcc cggagcagtt tgagccgtta
     5521 ttcggcttgc gagaagttga tggcagcgag ctgacgattg ttgaggagtg gtgctttggc
     5581 tatatgcggg gcgtggcact ttctgactgg tcaacgttgc ccgattcgtt aaaaccagcg
     5641 ctggaggcga ttgcgctgca cggtactgag gaaaacttcg agcgggtaga aaagatgtcg
     5701 ccagaagcgt ttgaagagag cgtagatgcc attcgactgg cggcgcttga tctacatgcg
     5761 tactggatgg cgcatccgca ggaaaaagct gttcagcaac cgatcaaagc agaagagaaa
     5821 ccggggcgta acgatccttg cccgtgcggt agtggtaaga aatttaagca gtgctgcctg
     5881 cattaagagg ccaaattggg tgtcagcgtt aattctggca cccaataagg aatttgaggc
     5941 tatctgatca ccccacttct ggtaacaacc ctgccgcaat caaaaattgc acgccaatca
     6001 cagcaatacc acagagaaac accaccacca gcgccggacg accacctttg acccggtaac
     6061 ccgcctgagg attgtgcttt ctgctttgcc aggtcaacag cgaagggata atcaatgcca
     6121 gtaccgccag cgccacaccg gcgtaaccca gcgccatcac aaatcctcgt ggataaaaca
     6181 gtgcaaacgc caacggcggc agaaaggtaa ttgcaccagt ttgcaaccgt ccaccaacgg
     6241 tatttgaacg ctgaaataaa tcagccagat aatcaaataa gcctaacgca acgccgagaa
     6301 atgacgtggc gagggctaaa tcagcaaata aatgcactgc cagctcaaca tgcggagagg
     6361 ccaccatttc gcgtaacgcc tgtaacagcc cgtttaatcc agcatgatta gccagcaatc
     6421 ccataaaggt tgttgaatca atgctgccaa gcgttgccac ctgccagaaa atatatgcca
     6481 ccagggggat cgcactaccg gttataaaca cccagcgtag cttacgaacg ttgccatcca
     6541 tatagctgac aatactcggc acgctaccgt gaaaaccaaa tgaggtaaaa atcaccggga
     6601 ttgctgacag agccagcccc tgttgcaacg gcagggttaa aagattcact ttgtgaatat
     6661 gcggtagcag caataccagc atcaccacca gaaaaataat tttggcgctg aacagaaaac
     6721 ggttaaataa atcgaccagc gatgttccaa cacaaaccac gccaccggca acaaaagtga
     6781 acaacagcac gccagcggtt gccgacattg aaatacctgt ccagtcgctg atgctggagg
     6841 ccaacaattc accgacaccg ctgatgtatg ccgcagtcag agcatacatt aagaacatca
     6901 tactgaagcc cgtcagccat tgaccgtagc gtcccagata gcgttttgcc agcgtgccca
     6961 gaccggtatc tgccggaaca tgctggtaca cctccagcag taatagcgcc gtgtagcaca
     7021 tcaacgccca aagcccaatc aacaagatta acgtaacgct aaaaccaaca ccggccgcag
     7081 ccagcggcat tgccagcatg cctgcgccaa ttgtggttcc cgccacgata aaaacacttc
     7141 ccagggttct gtttttcacg ctttcttctg tcctgacgat ctttatgagt gatatctgcg
     7201 gcgcaggtta aggcataacg gctgcttcgt caaaccgacg ttacatatag tgtaaatata
     7261 aatgtacaat aaaaagcatt cagtcctgcg ctggcgcaaa gtcttcataa gcggcaatcg
     7321 ctacgctagg ctttttttga ggaggaacga tggactctat tcacggtcat gaagtgttaa
     7381 atatgatgat tgaatcaggc gagcaatata cgcatgccag tctggaagct gcgattaaag
     7441 cgcgttttgg tgaacaggca cgttttcaca cctgctcggc agaagggatg acagcgggag
     7501 agctggtagc gtttctggca gcaaaaggca aatttatacc ttcgaaagac ggtttttcga
     7561 ccgatcagag taagatttgc cgtcactgat ttgaaacggc ggcagtaaac ctgccgccgg
     7621 agagcattag ttttgtgtgt cgagggtaga gagttctttg tcgataaaat acagaccttc
     7681 gccgcttttg cctgccaggc ttaatttatc aataatcgat ttgaacagtt tctcttcttc
     7741 atgctgctca gaaacatacc actgcaggaa attaaatgtt gggtagtcct gattggtcat
     7801 tgcagcatga gccagttcgt taattttctg ggtgattaat tgttcgtgtt tataggtttc
     7861 ctggaataat tcatcaagtg aggaatattc agcaaacgga gattcaacgg tattaatacg
     7921 cggtaaattg ccggtatcag tcaggtaatc aaacagacgc tgcatatgcg tcatctcttc
     7981 ctgggcgtga cggcgcagga acgcggcagc accttcgaag gtatgatagc tgcaccaggc
     8041 gctcatttgc tgataaagca gtgaagagta cagttccagg ttcatctgct cattaagttt
     8101 ttcaatcatt tctggtttca gcatgatagt gctccacaaa ggttatattt gttgctgtgg
     8161 cgtcactata atttgttatt ttattatttg caaaaggtaa aatataaact tcaattatta
     8221 aaaatacgaa t
//
LOCUS       DAZPLM010000183         8215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPLM010000183 DAZPLM010000000
VERSION     DAZPLM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.183"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHVC7_003428"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHVC7_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR2117389.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHVC7_003429"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHVC7_003429"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR2117390.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHVC7_003430"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHVC7_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHVC7_003431"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHVC7_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR2117391.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHVC7_003432"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHVC7_003432"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR2117392.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHVC7_003433"
     CDS             complement(6490..6846)
                     /locus_tag="ACHVC7_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR2117393.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHVC7_003434"
     CDS             complement(6850..7266)
                     /locus_tag="ACHVC7_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR2117394.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHVC7_003435"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHVC7_003435"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR2117395.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2041 a         2087 c         2047 g         2040 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagca
//
LOCUS       DAZPLM010000184         8195 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPLM010000184 DAZPLM010000000
VERSION     DAZPLM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.184"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHVC7_003436"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHVC7_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR2117396.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHVC7_003437"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHVC7_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR2117397.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHVC7_003438"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHVC7_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR2117398.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHVC7_003439"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHVC7_003439"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR2117399.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHVC7_003440"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHVC7_003440"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR2117400.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHVC7_003441"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHVC7_003441"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR2117401.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHVC7_003442"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHVC7_003442"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR2117402.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHVC7_003443"
     CDS             5315..6439
                     /locus_tag="ACHVC7_003443"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR2117403.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHVC7_003444"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHVC7_003444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR2117404.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>8195
                     /gene="waaO"
                     /locus_tag="ACHVC7_003445"
                     /gene_synonym="rfaI"
     CDS             7245..>8195
                     /gene="waaO"
                     /locus_tag="ACHVC7_003445"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR2117405.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFK"
BASE COUNT         2106 a         1887 c         2071 g         2131 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt ttaaa
//
LOCUS       DAZPLM010000185         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPLM010000185 DAZPLM010000000
VERSION     DAZPLM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.185"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHVC7_003446"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHVC7_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR2117406.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHVC7_003447"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHVC7_003447"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR2117407.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHVC7_003448"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHVC7_003448"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR2117408.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHVC7_003449"
     CDS             6679..>8182
                     /locus_tag="ACHVC7_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR2117409.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPLM010000186         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPLM010000186 DAZPLM010000000
VERSION     DAZPLM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.186"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHVC7_003450"
     CDS             <1..254
                     /locus_tag="ACHVC7_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR2117410.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHVC7_003451"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHVC7_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR2117411.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHVC7_003452"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHVC7_003452"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR2117412.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHVC7_003453"
     CDS             complement(2905..3180)
                     /locus_tag="ACHVC7_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR2117413.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHVC7_003454"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHVC7_003454"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR2117414.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHVC7_003455"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHVC7_003455"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR2117415.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHVC7_003456"
     CDS             5140..5466
                     /locus_tag="ACHVC7_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR2117416.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHVC7_003457"
     CDS             5650..6369
                     /locus_tag="ACHVC7_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR2117417.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHVC7_003458"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHVC7_003458"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR2117418.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHVC7_003459"
     CDS             7708..>8157
                     /locus_tag="ACHVC7_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR2117419.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZPLM010000187         8136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPLM010000187 DAZPLM010000000
VERSION     DAZPLM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.187"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHVC7_003460"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHVC7_003460"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR2117420.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="ACHVC7_003461"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="ACHVC7_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR2117421.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHVC7_003462"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHVC7_003462"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR2117422.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="ACHVC7_003463"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="ACHVC7_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR2117423.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="ACHVC7_003464"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="ACHVC7_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR2117424.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHVC7_003465"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHVC7_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR2117425.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHVC7_003466"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHVC7_003466"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR2117426.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2036 a         2085 c         2083 g         1932 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct tctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccccaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgttctcg
     3061 cgaacagcag ctggccatac accacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccaactga
     3241 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtacagggt tccacaccag aatatcgccg ttcagacccg catgacccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc gctcagcttg
     3421 ccgccaatcc cgacaaggaa taccgcgcca agatctttcg ctatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
     3541 tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacaa
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgctga agtcactgaa gcctattaca caacaggcca ctacagcatc
     4501 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccataatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggtagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
     6001 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
     6061 gcggtggtcg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
     6181 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccatcgatcg aagacaaagt catgcgcttc gccgacagaa
     6421 atcagcatca gatcttcctt gaaccggaag ggctgacctc taacgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatccac
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggtc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgagccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagctct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatcaa
//
LOCUS       DAZPLM010000188         8106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPLM010000188 DAZPLM010000000
VERSION     DAZPLM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.188"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            11..757
                     /locus_tag="ACHVC7_003467"
                     /pseudo
     CDS             11..757
                     /locus_tag="ACHVC7_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            1120..2157
                     /gene="dusA"
                     /locus_tag="ACHVC7_003468"
     CDS             1120..2157
                     /gene="dusA"
                     /locus_tag="ACHVC7_003468"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR2117427.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2291..2533
                     /gene="pspG"
                     /locus_tag="ACHVC7_003469"
     CDS             2291..2533
                     /gene="pspG"
                     /locus_tag="ACHVC7_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR2117428.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2699..3682)
                     /locus_tag="ACHVC7_003470"
     CDS             complement(2699..3682)
                     /locus_tag="ACHVC7_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR2117429.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3765..5180
                     /gene="dnaB"
                     /locus_tag="ACHVC7_003471"
     CDS             3765..5180
                     /gene="dnaB"
                     /locus_tag="ACHVC7_003471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR2117430.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5233..6312
                     /gene="alr"
                     /locus_tag="ACHVC7_003472"
     CDS             5233..6312
                     /gene="alr"
                     /locus_tag="ACHVC7_003472"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR2117431.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6565..7758
                     /gene="tyrB"
                     /locus_tag="ACHVC7_003473"
     CDS             6565..7758
                     /gene="tyrB"
                     /locus_tag="ACHVC7_003473"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR2117432.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1992 a         1905 c         2082 g         2127 t
ORIGIN      
        1 atgcacgtaa aaaaatgtgt ttttaattga gcaaataaat gatctcaaaa tgtgggtcaa
       61 taagtatatt gacgattgca ctgatgagga tttaaacgat cgtgacttta ttgcatcagt
      121 tgttgaccgg gctatttttc atttcgcgat taatagtata tgtaatcctg gggataataa
      181 agatgcgact cccattgaac aatgtacttt tgatgtagaa actaagaatg gccttccctc
      241 cacggttcag ctattttatg aggaatctaa ggataatgaa cctttagcga atatacattt
      301 tcaagcaata ggttctggtt ttttaacgtt tgttaatgcc tgccaggaac atgatgacaa
      361 cagcttaaaa ttatttgctt cgctgttaat ttcactttca tattctagtg cctacgcaga
      421 tttatcagaa acagtgtata ttaatgaaaa taatgagagc tacctgaaag ctcagtttga
      481 aaaattatct caacgtgata tgaagaagta cctgggagag atgaagcgtc tggctgatgg
      541 gggagaaatg aattttgatg gctatctgga taagatgtca catctggtga atgaaggaac
      601 gctcgatcct gatattttaa gcaaaatgcg agatgctgca ccacaattaa ttagcttcgc
      661 gaagtcgttt gacccaacct caaaggaaga gattaaaata cttacagaca cttctaaatt
      721 aatttatgat ttgttcgggg ttaaatcgga gaaataatat gtgaagttct tcgatagtat
      781 ggaaggcatt atataaaagg acccaatatt tattgggttc ttttttctct atcaatgcta
      841 ttagcaggga gatatatcac cagagtttaa tgtgtgattt tttatttatc gtcgaacctg
      901 gattgtttat cattggcctt aacaaagtta acggctaata agattatttc catcacttcg
      961 tgagagcttc atgccttgag aggatctcaa ttttcttttg caatgagaca ggcgcttcct
     1021 gttgttatgg tatagtaccc cgctattgag cctcctgaat agtgatgctg aataacataa
     1081 ccccatgata tatcgataaa ataatctcta catttgaaaa tgcacggtaa ttctgaaatg
     1141 caaaaaatca accaaaccag cgcaatgcct gaaaaaactg acgttcactg gagtggtcgg
     1201 tttagcgttg caccaatgct cgactggacg gacagacatt gccgctattt cttgcgtctg
     1261 ctttcccgca atacgttgct gtataccgaa atggtgacca caggggcgat tattcacggt
     1321 aaaggtgatt acctggcgta cagtgaagaa gaacatccgg tagcgttgca actcggcggt
     1381 agcgatccgg cggcgctggc acagtgtgcg aagctggcag aagcgcgtgg atatgatgag
     1441 atcaacctga atgtcggctg cccgtctgac cgggtgcaga acggcatgtt tggtgcgtgt
     1501 ctgatgggta atgcgcagct ggttgccgac tgcgtgaaag cgatgcgcga tgtggtgtcg
     1561 attccagtga cggtgaaaac gcgtattggc atcgatgacc aggacagcta tgaatttctc
     1621 tgcgatttca tcaacaccgt ttccggcaaa ggcgagtgtg agatgtttat catccacgca
     1681 cgtaaagcct ggctttcggg gttaagcccg aaagaaaacc gtgaaatccc gccgctcgat
     1741 tatccgcgtg tgtatcaact gaagcgtgac tttccgcatc tgacaatgtc gattaacggt
     1801 ggtatcaagt cgctggaaga ggccaaagca cacctgcaac atatggatgg cgtgatggtc
     1861 gggcgcgagg cgtatcagaa tccgggtatt ctggcggcgg tagaccggga gatctttggt
     1921 tcctcggata ccgatgccga tccggtggcg gtagtgcgcg ccatgtatcc gtacattgag
     1981 cgtgaactca gccaggggac gtatctcggc catattaccc ggcatatgtt gggcttgttc
     2041 cagggtattc ctggcgcgcg gcagtggcgg cgttatttaa gtgaaaatgc ccataaagcg
     2101 ggtgcagaca ttaatgtgct ggaacacgcg ctcaaactgg tggcggataa gcgttaactt
     2161 ttcaccaaaa agtagtcaaa ttcaccacgc cctgcgcacc gtcgcggggc gttttgctgt
     2221 taaatcaata gattattttt ggcatgattc ttgtaatgcc agcaagagat ttcatatttg
     2281 ggagagcatc atgctggaac tactttttgt gattggcttt tttgtcatgc tgatggtcac
     2341 cggcgtttcg ttgctgggca ttatcgccgc gctggttgtg gcgacggcca ttatgttcct
     2401 cggcggtatg ctggcattga tgattaagtt gctgccgtgg ttactactgg cgattgcggt
     2461 ggtgtgggtt attaaggcga ttaaagcacc aaaagtgccg aaatatcagc gttatgaccg
     2521 ctggcgttac taagggattg tgcggatgat cacaacctaa ggttttatcc ttagaacaaa
     2581 ataggaattg ataatcaaat ctgtcactat tgcgcctcta acagattcat cgtgctgtac
     2641 cctacataca gccgaactat aaaaagaaag ggcttcccag gtggaagccc tatttctttt
     2701 atggaatcaa caggctggaa ccttgcgtcg cccggctttc cagaatctca tgcgcacgct
     2761 gcgcatcctt cagcggatat ttctgctgct cggcgacatc gaccttaatc acaccgctgg
     2821 caatcaaaga gaacagttca ttactggcct cggttaattc ctcccgcgtg gtgatatagc
     2881 cttgcaggga agggcgtgtc acatacaacg agcctttttg attgagaatg cctaagttca
     2941 caccggtaac cgcacctgat gagttgccaa aactgaccat taagccgcgg cgttgcaggc
     3001 aatccagcga ccgttcccag gtgtctctgc ccacggaatc gtacaccacg cgcactttct
     3061 taccgccggt gatctctttt aaccgctcga ccagatcctc ttcacgatag ttaataacct
     3121 gccacgcgcc cgcttttagc gcgctctgcg ctttttgcgc ggttcctacg gtgccgataa
     3181 gtttcgcgcc cagggctttt gcccactggc aggcaattaa gccaacgccg ccagccgctg
     3241 cgtggaacag gaactgttca tcgggtttaa tttcataggt tttgcgcagc agataataaa
     3301 ccgttaagcc tttcaggaag gatgccgcag cttgctcaaa agaaattgcc gcaggcagaa
     3361 tcgccgcttt atccgcatta atgttatgca cagagctgta agcgcctaac gccgactgcg
     3421 catagactac acgatcgcct gccttaatat gctttacacc actgccgact ttactcacga
     3481 tgcctgccgc ttcggtacct aatccgctgg gtagtgatgg cggcgggtaa aggccgctgc
     3541 ggatatacgt gtcgataaaa ttgatgccga tggctttatt ttcgacctgg atttcattct
     3601 ctgccggatc ggcaggagtg aactctacgg cttgaagtac ttccgggcca ccgtgcttgt
     3661 gaaattcaat tcgtgttgcc atgtgtcctc cagaacgtat cgtcagggtc tgcttcatat
     3721 ggtaaagttt cgacccattc tttatctcgg taactccatt cactatggca ggaaataaac
     3781 ccttcaacaa acagcaggct gaaccccgcg aacgcgatcc acaagttgcc gggctgaaag
     3841 tgcctccgca ctcgatcgaa gcggagcagt cggtgttggg cggtttaatg ctggataacg
     3901 aacgctggga cgatgtagcc gagcgtgtgg tggcagacga tttttacacc cgcccacacc
     3961 gccatatctt tactgaaatg gcgcgtttgc aggaaagcgg tagtcctatc gatctgatta
     4021 cccttgcgga atcgctggaa cgccaggggc aactcgatag cgtcggtggt tttgcttatc
     4081 tggcagagct gtcaaaaaat acgccaagtg cggcgaacat cagtgcttat gctgacatcg
     4141 tgcgtgaacg tgccgttgtc cgtgagatga tctcggttgc gaatgagatt gctgaagccg
     4201 gttttgatcc gcaggggcgt accagcgaag atctgctgga ccttgctgaa tcccgcgtct
     4261 ttaaaattgc cgaaagtcgt gcaaacaaag acgaagggcc gaagaacatc gccgatgtgc
     4321 tcgacgcaac cgtggcgcgt attgagcagt tgtttcagca gccacacgat ggcgttaccg
     4381 gagtaaacac cggttatgac gatctcaaca aaaaaaccgc tggcttgcag ccgtcggatt
     4441 tgatcatcgt cgccgcgcgt ccgtcgatgg gtaaaacaac atttgcgatg aacctcgtcg
     4501 aaaacgcggc gatgttgcag gataaaccag tacttatctt ctcgctggag atgccttcag
     4561 aacagattat gatgcgttct ctggcgtcgc tgtcgcgcgt tgaccagact aaaatccgta
     4621 ccgggcagct cgatgatgaa gactgggcgc gcatttccgg caccatgggt attttgctcg
     4681 aaaaacgcaa tatctatatc gatgactcct ccggcctgac gccaacggaa gtgcgttccc
     4741 gcgcacgccg tattgcccgt gaacacggcg gcatcgggct tatcatgatc gactacctgc
     4801 aactgatgcg cgtaccggcg ctttccgata accgtacgct ggaaattgca gaaatctctc
     4861 gctcgctgaa agcactggcg aaagaactga acgtgccggt ggtggcgctg tcccagttga
     4921 accgttctct ggaacaacgt gccgacaaac gcccggtcaa ctccgacctg cgtgaatctg
     4981 gctctatcga gcaggatgcg gacttgatta tgtttatcta tcgtgatgag gtgtatcacg
     5041 aaaacagtga tttaaaaggc atcgcggaaa ttattatcgg taaacaacgt aacggcccaa
     5101 tcgggacggt acgcctgacc tttaacggtc aatggtcgcg cttcgacaac tatgcggggc
     5161 cgcagtacga cgacgaataa taattatttt atgaattagg taattaaagc aaacacttat
     5221 caaggaacac aaatgcaagc ggcaactgtt gtgattaacc gccgcgctct gcgacacaac
     5281 ctgcaacgtc ttcgtgaact ggcccctgcc agtaaaatgg ttgcggtggt gaaagcgaac
     5341 gcttatggtc acggtcttct tgagaccgcg cgaacgctcc ccgatgctga cgcctttggc
     5401 gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg gaatcaccaa acctgtactg
     5461 ttactcgaag gcttttttga tgccagagat ctgccgacga tttctgcgca acattttcat
     5521 accgccgtgc ataacgaaga acagctggct gcgctggaag aggctagcct ggacgagccg
     5581 gttaccgtct ggatgaaact cgataccggt atgcaccgtc tgggcgtaag gccggaacag
     5641 gctgaggcgt tttatcatcg cctgacacag tgcaaaaacg ttcgtcagcc ggtgaatatc
     5701 gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg caaccgagaa acaactcgct
     5761 atctttaata ccttttgcga aggcaaacct ggtcaacgtt ccattgccgc gtcgggtggc
     5821 attctgctgt ggccacaatc gcattttgac tgggtgcgcc cgggcatcat tctttatggc
     5881 gtctcgccgc tggaagatcg ctccatcggt gccgattttg gctgtcagcc agtgatgtca
     5941 ctaacctcca gcctgattgc cgtgcgtgag cataaagccg gagagcctgt tggttatggt
     6001 ggaacctggg taagcgaacg tgatacccgt cttggcgtag tcgcgatggg ctatggcgat
     6061 ggttatccgc gcgccgctcc atccggtacg ccagtgctgg tgaacggtcg cgaagtaccg
     6121 attgtcggac gcgtggcgat ggatatgatc tgcgtagact taggtccaca ggcgcaggac
     6181 aaagccgggg atccggtcat tttatggggc gaaggtttgc ccgtagaacg tatcgctgaa
     6241 atgacaaaag tgagcgctta cgaacttatt acgcgcctga cttcaagggt cgcgatgaaa
     6301 tacgtggatt aatcgttctg taatatttga ttgtctgtgc cggatgcggc gtgaatgcct
     6361 tatccggcca ataaaatcct aaaaattcaa taagttgatg ttctttcatg ctcttataaa
     6421 ggtcgtgcct ctggcggatg ttcgtttgtc atgagtctca ctctgttgct aattgccgtt
     6481 cgctcctgaa catccactcg atcttcgcct tcttccggtt tattgtgttt taaccacctg
     6541 cccgtaaacc tggagaacca tcgcgtgttt caaaaagttg acgcctacgc tggcgacccg
     6601 attcttacgc ttatggagcg ttttaaagaa gaccctcgca gcgacaaagt gaatttaagt
     6661 atcggtctgt actacaacga agacggaatt attccacaac tgaaagccgt ggcggaggcg
     6721 gaagcgcgcc tgaatgcgca gccccatggc gcttcgcttt atttaccgat ggaagggctt
     6781 aacagctatc gccatgccat tgcgccgctg ctgtttggtg cggaccatcc ggtactgcaa
     6841 caacagcgcg tagcaaccat tcaaaccctt ggcggctcag gggcattgaa agtgggcgcg
     6901 gatttcctga aacgctactt cccggaatca ggcgtctggg tcagcgatcc tacctgggaa
     6961 aaccacgtag caatattcgc cggggctgga ttcgaagtga gtacttaccc ctggtatgac
     7021 gaagcgacta acagcgtgcg ctttaatgac ctgttggcga cgctgaaaac attacctgcc
     7081 cgcagtattg tgttgctgca tccatgttgc cacaacccaa cgggtgccga tctcactaat
     7141 gatcagtggg atgcggtgat tgaaattctc aaagcccgcg agcttatccc attcctcgat
     7201 attgcctatc aaggatttag tgccggtatg gaagaggatg cctacgctat tcgcgccatt
     7261 gccagcgctg gattacccgc tctggtgagc aattcgttct cgaaaatttt ctccctttac
     7321 ggcgagcgcg tcggcggact ttctgttctg tgtgaagatg ccgaagccgc tggccgcgta
     7381 ctggggcaat tgaaagcaac agttcgccgc aactactcca gcccgccgaa ttttggtgcg
     7441 caggtggtgg ctgcagtgct gaatgacgag gcattgaaag ccagctggct ggcggaagta
     7501 gaagagatgc gtactcgcat tctggcaatg cgtcaggaat tggtgaaggt attaagcaca
     7561 gagatgccag aacgcaattt cgattatctg cttaatcagc gcggcatgtt cagttatacc
     7621 ggtttaagtg ccgctcaggt tgaccgacta cgtgaagaat ttggtgtcta tctcatcgcc
     7681 agcggtcgca tgtgtgtcgc cgggttaaat acggcaaatg tacaacgtgt ggcaaaggcg
     7741 tttgctgcgg tgatgtaatg caggaaagca ggctggagtt acccagcctg cagtgaaatt
     7801 aaactgtcgt cgctttcact atttctttgt agatgatttt tttgatgccg ttgttctacg
     7861 tgagagataa taaaacgttg tcagttcttt tattgttaag tttatcccaa ttatctggaa
     7921 ttcctttccc tattttttcg tgtggagtaa taatttctgc acatctaacc attattaaat
     7981 ttaataataa gtatcgggtt tgttacgata gaatatttct atatcctcaa gaatatactt
     8041 tgccttattg cctgtgactt tctgagattc aattatagta tcgttaaatt ctaatgttaa
     8101 agagaa
//
LOCUS       DAZPLM010000189         7974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPLM010000189 DAZPLM010000000
VERSION     DAZPLM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.189"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHVC7_003474"
     CDS             <1..643
                     /locus_tag="ACHVC7_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2117433.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHVC7_003475"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHVC7_003475"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR2117434.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHVC7_003476"
     CDS             2474..2986
                     /locus_tag="ACHVC7_003476"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR2117435.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHVC7_003477"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHVC7_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR2117436.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHVC7_003478"
     CDS             5579..5782
                     /locus_tag="ACHVC7_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR2117437.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHVC7_003479"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHVC7_003479"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR2117438.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..7723)
                     /locus_tag="ACHVC7_003480"
                     /pseudo
     CDS             complement(6827..7723)
                     /locus_tag="ACHVC7_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT         1834 a         2173 c         2097 g         1870 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatctgc cccgttactt tattcgtacc
     7741 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
     7801 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
     7861 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
     7921 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaa
//
LOCUS       DAZPLM010000190         7965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPLM010000190 DAZPLM010000000
VERSION     DAZPLM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.190"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHVC7_003481"
     CDS             complement(152..487)
                     /locus_tag="ACHVC7_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR2117439.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHVC7_003482"
     CDS             complement(491..835)
                     /locus_tag="ACHVC7_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR2117440.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHVC7_003483"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHVC7_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR2117441.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHVC7_003484"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHVC7_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR2117442.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHVC7_003485"
     CDS             1257..1376
                     /locus_tag="ACHVC7_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR2117443.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHVC7_003486"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHVC7_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR2117444.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHVC7_003487"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHVC7_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR2117445.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHVC7_003488"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHVC7_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR2117446.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHVC7_003489"
     CDS             4176..4544
                     /locus_tag="ACHVC7_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR2117447.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHVC7_003490"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHVC7_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR2117448.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHVC7_003491"
     CDS             5295..5588
                     /locus_tag="ACHVC7_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR2117449.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHVC7_003492"
     CDS             5660..6319
                     /locus_tag="ACHVC7_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR2117450.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHVC7_003493"
     CDS             6411..6857
                     /locus_tag="ACHVC7_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR2117451.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7965)
                     /gene="hlyE"
                     /locus_tag="ACHVC7_003494"
     CDS             complement(7064..>7965)
                     /gene="hlyE"
                     /locus_tag="ACHVC7_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR2117452.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFF
                     TTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKK
                     MINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         2049 a         1789 c         1836 g         2291 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacga
//
LOCUS       DAZPLM010000191         7943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPLM010000191 DAZPLM010000000
VERSION     DAZPLM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.191"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHVC7_003495"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHVC7_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR2117453.1"
                     /translation="MCEGYVEKPPYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHVC7_003496"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHVC7_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR2117454.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHVC7_003497"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHVC7_003497"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR2117455.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHVC7_003498"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHVC7_003498"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR2117456.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(3836..5053)
                     /gene="caiB"
                     /locus_tag="ACHVC7_003499"
                     /pseudo
     CDS             complement(3836..5053)
                     /gene="caiB"
                     /locus_tag="ACHVC7_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            complement(5182..6324)
                     /gene="caiA"
                     /locus_tag="ACHVC7_003500"
     CDS             complement(5182..6324)
                     /gene="caiA"
                     /locus_tag="ACHVC7_003500"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR2117457.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(6355..7869)
                     /gene="caiT"
                     /locus_tag="ACHVC7_003501"
     CDS             complement(6355..7869)
                     /gene="caiT"
                     /locus_tag="ACHVC7_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR2117458.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
BASE COUNT         1902 a         2143 c         1980 g         1918 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc accctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg ccaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagacct ttgctgacca actcctgaat gtcgttttcg ctgtagccga
     3901 tatttttcaa aatggcagcc gtgtccatgc catgtgaggg cattccgcgc cagatttgtc
     3961 cggggttatt tttgaatttc ggcatgatgt tcggcccttt gcaggtgcga ccatccatcg
     4021 tttgccactg agtgattgat tcacgggcaa catactgtgg attgctttcc agttccggta
     4081 cggtcagtac tttggcacag gcgatattca gttcggcaaa gcgttcttta acttctgcga
     4141 tggtatgtgc cgccagccag gcatcgagtt tttcttctac cagtgggccg taagggcatt
     4201 cgatacggtg gataagctga gtgccttccg ggatttccgg cgtaccgagt agatgtgcga
     4261 ggccaatatc tttaaagcac tcttcaattt gggtaatgcc caccagttcc atcacgatgt
     4321 agccgtcggc gcatttatac agaccgcaac cggcgtagta gggatcttta cctttgctca
     4381 tgcgcgggca catttcgccg ccgttgaagt aatccatcat gaagtactgg cccatacgca
     4441 gcatcacttc atacatggcg atgtcgatac tttcgccttt accggtttca cgcactttat
     4501 gcagtgctgc cagcgccgcc gtggtggcgg tcaggccaga aaagtaatcg gcggtatacg
     4561 ggaaggcagg cattggctgg tcaacatcac cgttctgaat caggtaacca ctgaaggcct
     4621 gggcgatggt gttataggcc ggaagattgg tgtactcctc ggtgccgtac tgaccaaaac
     4681 cggacaggtg agcgataacc agtttcgggt tgtgctgcca cagtacttca tcggtaatgc
     4741 cacgacgggc aaaggccgga cctttgctgg cttcgatgaa gatatcggtg gtttccatta
     4801 atttcagaaa cgcttcgcgg ccttcatctt tgaaaatatt taacgacagc gcgtgcaaat
     4861 tgcggcggga gagctgcggg tagttcggtt gaacgcgaat ggtgtcggcc taggcgacgt
     4921 tctcgatcca gataacttcc gcaccccatt ctgcgaacat ttgcccggcg aacggcccgg
     4981 cgatttcgat accggagaag acaacgcgca atccggccaa cggcccgaat ttcggcatgg
     5041 gtagatgatc cattattagc tcctgaaaaa tttatgtagc gcatgactgc cggatgcggc
     5101 gtaaacgctt tatccggcct acattcgtgc tcccgtaggc ctgataagac gcattagcgt
     5161 cgcatcaggc agcgcacgga cttagcggta ttgcttcagc accgcgcgac ccagcgtcag
     5221 gatctgcatt tcgtcagatc ccccggagac gcggtctaca cgcagatcac gccagaagcg
     5281 gctgatgcgg tggttgcccg caatcccgac accgcccagc acctgcattg cgctatccac
     5341 aacttcaaat gccgcattgg cgcagaagta tttgcacatc gctgcatcgc cagaggtgat
     5401 ggtgccgttg tctgctttcc acgctgcttc atacagcatg tttttcatgg agtttaattt
     5461 gatcgccatg tgggcgaatt tttcctgaat caactggaaa cgaccaatag cctcgccaaa
     5521 ctgcacgcgc tgattggcgt agcgcgccgc atcttcaaaa gcgcacatcg ccgtaccgta
     5581 gttggtgagg gctaccagga aacgttcatg gtcgaactct tctttgacgc ggttaaagcc
     5641 gttaccttcc cgaccgaaca tgtctttctc gtccagttcc acgtcgtcaa aggtgatttc
     5701 acagcagcta tccatacgca gaccgagctt ttcaagtttg gtcactttga tgcccggttt
     5761 gctcatatca acaaaccatt cggtgtagac aggtttgtcc ggagaagccc cgtcgcgcgc
     5821 catcaccacg atgtacgggg tgtaggcgct gctggtaata aaacacttac taccattaag
     5881 ataaatctta ccatttctac gggtataagt cgttttcagg ctacccacgt cggagcccgc
     5941 gcccggttcg gtaatcgctg agttccacat ctgcttaccg gtgccgcgga aagccataat
     6001 tttgtcgatc tgctcttgtg tgccttcgcg caggaaggtg ttgaacccgc ccggcaactg
     6061 gtacagcaca taggttggtg cccccagacg tcccagctcc atccacacgg cggcgagagt
     6121 aacaaacccc gcgtccagac caccgtgctc ttcagggatc agcagactgt cgatacccat
     6181 atccgccagt gctttgacaa aacgttccgg gtagacgctg tcacggtcgc actcggcaaa
     6241 ataggcctcc cagttttcgc tggccatcag ttcgcggata ccggcgacaa acagttcctg
     6301 ctcatcattt aaattaaaat ccatctttca acctcttgat attttggggg ttaattaatc
     6361 tttccagttc tgtttcgcgt ctttaataaa ggagagcgtc accataatgt tgacgaagaa
     6421 cagcgggcat cctccggcga taatggcggt ttgaatcggt ttcaggccgc cgagcgccag
     6481 cagaacaata ccgataatgc caaccagaat tgaccaaccg atacgcacca gcagaggtgg
     6541 ttcttcacca tcgcgtactt cgcggcaagt ggacatcgcc agggtataag agcaggcgtt
     6601 aaccagcgta acggtggcaa taaagcagag gatgaagaag ccccacatgg tggcggtgct
     6661 gagtggcaga gcggcccagg tttcaatgat ggcgcgcgcc acaccgtact gttcgatcag
     6721 atttggaatg ttgatgatgt ttttatctat caacagcaga gtgttactac cgagtacagt
     6781 ccacaggatc caggttgacg ctgtcagccc cagcaccatg ccgaagcaca gttcacgcac
     6841 agtacgacca cgggagatgc gggcgaggaa gatactcatc tggatagcat aaatcaccca
     6901 ccatgcccag tagaacacgg tccagccctg cgggaagccg cctttagcga tgggatcggt
     6961 atagaacaac atgcgcggca gatacatcag caacatcccc accgaatcgg tgaagtagtt
     7021 catgatgaag ctggcaccgc tgacaatgaa cacccaaccc agcatcagga agctcaggta
     7081 actacgcacg tcactggcga tacgtacccc tttttgcaga ccgcaagcga cgcaaatggc
     7141 gttgaggata atccagcagg taatgatgat agcgtccagt tgcagggtat gcggaatgcc
     7201 aaacaaccat tgcatacact cggtcaccag cggcgtggca aggcccagac tggtacccat
     7261 cgcgaagatc aaggcgacga gatagaagtt gtcgacgata gtgccgaaca accctttggc
     7321 gtgtttttca cctaccagcg gcaccagtgt cgagctgggg cgaatcactt ccattttgcg
     7381 gacaaagaag aagtaagcga aggcgactga aaggaagctg taagtggccc acggcagagg
     7441 tccccagtgg aacaagctgt aagccagccc caactctttc gcccctgtcg agttcggttc
     7501 taagccaaac ggcggggtgg agatgtagta gtagatctca atcgatcccc agaacagtac
     7561 ggcagcagac gtacaggagg cgaacatcat aaagatccaa ctggcggtac taaattcagg
     7621 tggttcgtta cctaaacgct ttttggcata cgggccaaac accagccaga accaaccgaa
     7681 aagcatcacc accatatacc attcaaatgc ccatccccat acattggtga cgtaactgaa
     7741 tacagcatta ataacgacat tcgctgcatc cagatctctg actgtaagcc aacaaagtat
     7801 gccgacgatt attaacggcg gaaagaaaac cttcggttct attcccgttt ttctcttttc
     7861 attcttcatg agttaattcc actgtgaaaa cgaatattta ttttgcgttc ccgtttgttt
     7921 tatttttgtt aacatttgat ata
//
LOCUS       DAZPLM010000192         7936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPLM010000192 DAZPLM010000000
VERSION     DAZPLM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.192"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            98..187
                     /gene="kdpF"
                     /locus_tag="ACHVC7_003502"
     CDS             98..187
                     /gene="kdpF"
                     /locus_tag="ACHVC7_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR2117459.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            187..1860
                     /gene="kdpA"
                     /locus_tag="ACHVC7_003503"
     CDS             187..1860
                     /gene="kdpA"
                     /locus_tag="ACHVC7_003503"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR2117460.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1883..3931
                     /gene="kdpB"
                     /locus_tag="ACHVC7_003504"
     CDS             1883..3931
                     /gene="kdpB"
                     /locus_tag="ACHVC7_003504"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR2117461.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3940..4512
                     /gene="kdpC"
                     /locus_tag="ACHVC7_003505"
     CDS             3940..4512
                     /gene="kdpC"
                     /locus_tag="ACHVC7_003505"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR2117462.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4505..7189
                     /gene="kdpD"
                     /locus_tag="ACHVC7_003506"
     CDS             4505..7189
                     /gene="kdpD"
                     /locus_tag="ACHVC7_003506"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR2117463.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7186..7863
                     /gene="kdpE"
                     /locus_tag="ACHVC7_003507"
     CDS             7186..7863
                     /gene="kdpE"
                     /locus_tag="ACHVC7_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR2117464.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1618 a         2085 c         2354 g         1879 t
ORIGIN      
        1 tatacttttt ttacaccccg cccgcagatt tttgcgaaat ctttgcagcc agaattctac
       61 tcttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg tgatagccgg
      121 cgtgttgctg gtgtttttat tactgggtta tttggtttat gccctgatca atgcggaggc
      181 gttctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg
      241 ctggcgcgtc ctttaggcag cgggctggcg cggctgatta atgacattcc tcttccaggt
      301 acagcgggcg ttgaacgtat actctttcgt ttacctggcg tctctgacca tgagatgaac
      361 tggaagcaat atctttgtgc cattctcggc ctgaacatgc tggggctggc ggtgctgttt
      421 tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg
      481 tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg gcaatcttat
      541 agcggtgaaa ccacgttgag ctatttcagc cagatggcag gcttaacggt acaaaacttt
      601 ctttctgccg ccagcgggat tgcggtgatt tttgccttca tccgtgcgtt tactcgccag
      661 agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgcta
      721 gtccctgtgg cgttgttgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt
      781 ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc catgggacca
      841 gtagcttctc aggaagcgat caagatgctc ggtactaacg gcggtggctt ctttaatgcc
      901 aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg
      961 atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtgacggg cgatcgccgc
     1021 caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtt
     1081 atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga cagcagcatc
     1141 aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg
     1201 actacggcgg cgtcctgtgg cgcggtgatt gcgatgcatg attcgtttac cgctctcggc
     1261 ggcatggtgc cgatgtggct aatgcaaatt ggtgaagtgg tgttcggcgg tgtcggttct
     1321 ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt
     1381 ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgattgca
     1441 ctggcgattc tggttactcc aacactggta ctgatgggcg cggcgttggc gatgatgacc
     1501 gacgccggac gtagcgccat gcttaaccct ggcccgcatg gttttagcga agtgctgtac
     1561 gccgtgtcgt ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct
     1621 ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc
     1681 ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc
     1741 acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt
     1801 ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga
     1861 tgatattgag cgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact
     1921 tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa
     1981 tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat
     2041 ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg
     2101 ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca
     2161 ggccaacagt cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa
     2221 atatggcgct gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg acatcgtgct
     2281 ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt
     2341 cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt
     2401 tgcctccgtc accggcggca cgcgtattct ttctgactgg ctggtgattg agtgtagcgt
     2461 taaccccggt gagacatttc tggatcggat gatcgcgatg gtggaaggcg cacagcgacg
     2521 caaaacaccg aacgagattg ccctgactat cctgctgatt gctctgacca tcgtcttttt
     2581 actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgccg tgagtgtaac
     2641 ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc
     2701 gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca ccagcggacg
     2761 tgcagttgaa gcggcaggtg acgttgacgt tctgctactg gataaaaccg gcaccatcac
     2821 actcggtaac cgtcaggcgt cggagtttat ccccgcgcag ggcgtggatg aaaaaacgct
     2881 ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggtc gcagtattgt
     2941 gatcctcgcc aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac
     3001 ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat
     3061 ccgtaaaggt tctgtcgatg ccattcgccg ccatattgag gctaacggcg gtcacttccc
     3121 tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggagccacgc cgctggtggt
     3181 ggtggaaggt tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat
     3241 taaagagcgc ttcgcccagc tgcgcaaaat gggcattaaa acggtgatga ttaccggcga
     3301 taaccgtctg actgccgccg cgatagctgc ggaagcgggt gtcgatgatt ttctcgccga
     3361 agcaacaccg gaggccaagc tggcattgat ccgtcagtat caggcggaag gtcgtttggt
     3421 agcgatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag atgtcgcggt
     3481 ggcgatgaac tccggcaccc aggcggcgaa agaggcgggc aatatggtgg atctcgactc
     3541 taacccgacc aagttgatcg aggtggtgca cattggcaaa cagatgctga tgacccgtgg
     3601 ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccggc
     3661 ggcgttcgcg gcaacatatc cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc
     3721 cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct ttttgattcc
     3781 tctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa
     3841 cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga
     3901 tttactgctg accgtttgcg gtctggtgtg aggtttacca tgagtggatt acgtccggca
     3961 ttatcgacat ttctctttct gttattgatt actggcggcg tttacccgct gctgaccacc
     4021 gcactggggc aatggtggtt tccctggcag gccaacggtt cgttgatccg tgaaggtgat
     4081 acggtgcgtg gctcggcatt aatcgggcag aattttaccg gcaacggcta ttttcatggt
     4141 cgcccgtcag caacggctga aatgccctat aatccacagg cttctggcgg gagcaatctg
     4201 gcggtcagta accctgagct ggataaacta atagccgcac gcgttgctgc attacgggcc
     4261 gctaacccgg atgccagcgc gagcattccg gttgaactgg tgacggcatc ggcaagcggg
     4321 ctggacaata atatcacccc gcaagcggcg gcctggcaga tcccacgcat cgcgaaagcg
     4381 cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca acaaccgctg
     4441 gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc gctggataag
     4501 cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc
     4561 gccgcatcgg gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg
     4621 ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt
     4681 ggtagaaacc cacgggcgaa aagataccgc cgcaatgctg gaagggctgg ctgttctgcc
     4741 gccaaaacgc ctggcgtacc gtgggcggca catctgcgag tttgacctcg atgccgccct
     4801 cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc
     4861 tcgtcaccct aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt
     4921 cactaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg
     4981 aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggttct
     5041 ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc
     5101 cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg
     5161 cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg
     5221 gcatcctagc gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcggcca
     5281 taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggtag
     5341 cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg
     5401 tcgtgcaatt ctcagcgcct tacgtctggc gcaggaactg ggcgcggaga cggcaacact
     5461 ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata atctcggcaa
     5521 gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg
     5581 actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc
     5641 ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat
     5701 tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg ccatgcagtg
     5761 gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt
     5821 ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg tggtgagttt
     5881 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     5941 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg
     6001 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6061 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6121 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     6181 gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc agtggagttt
     6241 tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat
     6301 tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc cggggaatct
     6361 gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc
     6421 caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga tggcaagcga
     6481 acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac gcacgccgct
     6541 tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcacc
     6601 ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa
     6661 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     6721 aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc
     6781 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga
     6841 acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat
     6901 tggtatcgat gcccactttg agggcgaaaa tctacaactg gatgtctggg ataacggccc
     6961 cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga
     7021 gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg tggatgtaca
     7081 cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact
     7141 tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac aaacgttctg
     7201 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg
     7261 atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc aacccgtaag
     7321 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     7381 gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc
     7441 gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt
     7501 ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc
     7561 gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg
     7621 ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt gctgctcaac
     7681 aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg
     7741 gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa actggaacag
     7801 gatccggccc ggcctcgcca cttcattact gaaaccggta ttggatatcg gtttatgctt
     7861 tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa acctgcgccg
     7921 aatattatta tttggc
//
LOCUS       DAZPLM010000193         7880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPLM010000193 DAZPLM010000000
VERSION     DAZPLM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.193"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHVC7_003508"
     CDS             <1..365
                     /locus_tag="ACHVC7_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR2117465.1"
                     /translation="ITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERL
                     GERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFL
                     LRKDIMRRLTQDVCEEIE"
     gene            complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHVC7_003509"
     CDS             complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHVC7_003509"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR2117466.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHVC7_003510"
     CDS             3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHVC7_003510"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR2117467.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4123..5169
                     /gene="aroH"
                     /locus_tag="ACHVC7_003511"
     CDS             4123..5169
                     /gene="aroH"
                     /locus_tag="ACHVC7_003511"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR2117468.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5301..5492
                     /gene="hemP"
                     /locus_tag="ACHVC7_003512"
     CDS             5301..5492
                     /gene="hemP"
                     /locus_tag="ACHVC7_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR2117469.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHVC7_003513"
     CDS             complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHVC7_003513"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR2117470.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHVC7_003514"
     CDS             complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHVC7_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR2117471.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2016 a         2071 c         1873 g         1920 t
ORIGIN      
        1 aaattaccgg acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg
       61 aagtagaaga tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc
      121 ccgatgaacg tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt
      181 cattatcgct ggaagaggtg gtggcttttt ttagccgtaa acgggtcgca aaatataaat
      241 atcctgaaca tatcgtggta atcgaaaaac taccgcgtac tgcctcaggt aaaatacaaa
      301 agtttttgtt aagaaaagat attatgcggc ggttaactca ggatgtctgt gaagagattg
      361 aataagtttc atcttcgggg atcacataac cccggcgact gaacgccgcc ggggatttat
      421 tttatttctt cagttcagcc aagcttaacc aggtttgcac cacggtgtcc gggttcagag
      481 acaggctatc gatcccctct tccatcaacc atgcggcaaa gtcttcgtgg tcggacggac
      541 cctgaccgca aatcccgaca tatttgccct gtttcttcgc ggcacggata gccatcgaca
      601 gcagtgcttt caccgcatcg ttgcgctcat cgaacaattc agacaccacg ccggagtcac
      661 ggtccagacc gagcgccagc tgcgtcatat cgtttgagcc aattgagaag ccgtcgaaat
      721 attcgaggaa ctgctcggcc agcaaggcgt tggacgggat ttcacacatc atgatgattt
      781 tcagcccgtt ctcaccacgc ttcagcccct gacgcgccag ttcctcaacc accgctttcg
      841 cctgatctac ggtacgcacg aacgggatca tgatctcaac gttggtcagc cccatgtcgt
      901 tgcgcacacg tttcactgct tcgcactcca gcgcgaagca gtcgcggaag ctgtcggaaa
      961 catagcgtcc cgcgccacgg aagccgagca tcgggttctc ttcatctggc tcgtaacgct
     1021 caccaccgac caggttggca tattcgttcg atttaaaatc agagagacgg acaatgacgc
     1081 gcttcggata aaacgcggca cccagcgtcg cgatcccttc agtcagacga ccaacgtaaa
     1141 attcacgcgg agaatcaaaa cctttcatca tctcgcggat ttcattttgc aactgcggtt
     1201 cctgatcgtc aaactcaagc agtgcgcgtg ggtggacgcc aatcatacgg ttgatgataa
     1261 attccagacg cgcaaggccc acgccttcgt tcggcaggca ggcgaagtcg aaagcacggt
     1321 ccgggttacc gacgttcatc atcactttca acggcagatc cggcatcatt tctacgctgg
     1381 agcttttcac gctaaattcc agcaactccg catagacgta accggtatca ccttcggcac
     1441 aagaaacagt gacgttctca ccgtctttca tccgttctgt tgcatcacca cagcccacta
     1501 ccgccggaat gcccagttca cgagcgatga tcgccgcgtg acaggtacga ccgccacggt
     1561 tggtgacgat ggcagatgct ttcttcatga tcggttccca gtccgggtcg gtcatgtcag
     1621 tgaccagcac gtcaccaggt tcgatgcggt tcatttcgct gatctcatgg atgactttca
     1681 ccggacccgc gccgatgcga tgaccgatag cacggccttc ggcgataatc ttaccctgtg
     1741 aatgcagcgt ataacgctcc atgacctgac cgcgtgagcg cacggtttcc ggacgcgcct
     1801 gcacaatgaa cagtttgcca gtgtggccat ctttcgccca ctcaatatcc atcgggcgac
     1861 cgtagtgttt ctcaatttgt acggcctgtt ttgccagttc ctgcacttct tcgttggtca
     1921 gcgagaagat gtcacgctgt tcctgcggta cgtcttcgat tttaacctgc ttgccgtgct
     1981 cctgggtcgg cgcgtaaacc atgcggattt ttttcgaccc catggtgcga cgcacgatag
     2041 ccgggcgatt cgccgccagt gtcggtttat ggacgtaaaa ctcatccggg ttaaccgcac
     2101 cctgcacgac catttcccca aggccccatg cggaagtgat aaacaccacc tggtcaaagc
     2161 cggattcggt atcaatggag aacatcacgc cagaagatgc gaggtcggag cgcaccatcc
     2221 gctgaacacc ggcagagagc gccacgccac ggtgatcgta accctggtgc acacgataag
     2281 agatggcgcg atcgttaaac agagaagcaa atacatgctt cactgccacg agaacggcgt
     2341 caaaaccctg aacgttgagg aaggtttcct gctgaccggc aaaagaagcg tccggcatat
     2401 cttctgcggt ggcggaggag cgcaccgcaa aagaggcgtt ttcgtcatcg gcggaaagct
     2461 gtgcataggc ttcgcggatg gcgttttcca gctcaggctg gaagggagtg tcgataatcc
     2521 actggcggat ttgcgcgccc gctttcgcaa gctgagtaac atcgtcaata tccgttttat
     2581 ccagcagttc ataaatgcgc tggtttacgc cgctttggtc cagaaactgg ttaaacgcgt
     2641 cggcggttgt ggcgaaacca ttcggaacgg aaacacccat tccggaaagg ttagtaatca
     2701 tttcacccag ggaggcattt ttgcccccaa ccctgtctac atcattcatg ccgagttggt
     2761 tataccaaag caccagcggt gacgagccat tgttggacat cgaacaatcc ttttgtgata
     2821 aatgaacggt ttgagaaaca catttctgcg catttatctt tgcatattta accggatgaa
     2881 aaaaacggtg aatcgttcaa gcaaatatat ttttttactt tttaagactg atcccagcgt
     2941 tgcgcaaatc tgctctcttc gacaatttcc acataaacca tcggtataaa cgaaaccata
     3001 aaaatgaaat gctgttttca taaaaaataa aattgaaggt tcattttata aaccagacat
     3061 aacgtcctac gcttctgtgc gtttttaatt tatgctttca tagaattatg tctgcatcac
     3121 gggaagaaca aaatggataa tgctgttgat cgccacgttt tttatatttc tgatggtacg
     3181 gcaataactg cggaggtatt aggacacgca gtaatgtcac aatttcccgt cactatcagc
     3241 agcattacgc tgccgtttgt cgaaaatgag agccgtgcac gggcagtgaa ggatcagatt
     3301 gacgcaattt atcaccagac aggtgtgcgc ccgctggtct tctactccat cgtgttgccg
     3361 gagattcgcg ccatcatctt gcaaagtgaa ggcttttgcc aggatatcgt tcaggcgctg
     3421 gttgccccgc tacaacaaga gatgaaactg gatccaacgc cgattgctca tcgtacccat
     3481 ggccttaacc ctaataatct caataaatat gatgcgcgca ttgcggcgat tgattacacc
     3541 ctcgcccacg atgacggcat ttcgttgcgc aatctggatc aggctcaggt gatcctgctc
     3601 ggtgtttctc gctgtggtaa aacccccacc agtctttatc tggcaatgca atttggtatc
     3661 cgcgcggcaa actacccctt tattgccgac gatatggata atctggtgct acccgcgtcg
     3721 ctcaaaccgc ttcagcataa attgttcggc ctgactatcg acccggaacg tctggcggcg
     3781 attcgcgagg aacgtcggga gaacagtcgc tatgcctcgc ttcgtcagtg caggatggaa
     3841 gtcgcggaag tggaagcgtt gtaccgtaaa aatcagatcc cgtggattaa cagtaccaat
     3901 tattcggtag aagagattgc caccaagatc ctcgatatca tgggccttag tcgccgaatg
     3961 tactagagaa ctagtgcatt agcttatttt tttgttatga tgctaaccac ccggcgaggt
     4021 gtgacacacc tcgcacttga aatcagcagc gattggttta tcgtgatgcg catcacttcc
     4081 cggcagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     4141 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     4201 gtaacgcctg gcgccgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactc
     4261 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     4321 gctgcaatgg agtacgcccc ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     4381 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaaggacta
     4441 atctccgatc cagatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     4501 aaattacttt tacaggtaaa tgagctgggc gtcccaaccg cgactgagtt cctcgatatg
     4561 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc acgtactacc
     4621 gaaagtcaga tccaccgtga aatggcttcg gcactctcct gtccggtagg ttttaaaaat
     4681 ggtaccgatg gcaatacgcg gattgctgtg gatgctatcc gcgcagcccg cgccagccat
     4741 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     4801 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     4861 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     4921 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     4981 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgctcgaa
     5041 ggaacgcaaa aaatcgtcgg cagtcagccg ctcacttacg gtcaatccat taccgacccg
     5101 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     5161 cgcttctgaa tgcgtgccca ttcctgacgg aatgggcatt tctgcgcaac ttgttgtctt
     5221 ctcaacaaat tactgcttgc tctggcctgc cataatattg ataataagaa tcattgttat
     5281 atcaattatt attaatcttt atgcgttata cggatagcag aaaactcacg cctgaaacgg
     5341 acgccaatca caaggccgct tccccgcagc ccatccggcg aattaccagc cagacactgt
     5401 taggtccgga tggcaaactg attatcgatc atgacgggca agaatatctg ctccgtaaaa
     5461 ctcaggctgg caagctgctg ttaactaagt agcctttaac tcgagcagct gacttccagc
     5521 cgtttacccc agtcaggtgg acgactgaca tagtcatcat ctctatcgct gaaaggattt
     5581 cgcaacgccc catgcaggcg gtgcaattcc gtcatatcac ccttttctgc cgcttcaatc
     5641 gcccgttgcg ccaaccagtt gcgcaatacc agagcggggt taacgctttg catcaactgc
     5701 tgacgctcac tatcgctaac ctcgtcttgt tgcaaacgcc cccgataacg ggcaaaccag
     5761 ccatcaaatg ccgcacgatc aataaactca tcacgtagcg gtgacgccgt gctgtgctgc
     5821 tcggtcagac tcagcatgcg gaatgtgcgg gtataatcgc tgcgctctcg cgccatcaga
     5881 ctgaataatt cattcagtag cgcgttatcc tctttttgct ccgtgatgaa gcccagtttc
     5941 tgccgcatcc gttgtccata atgcgtcaac aaaacctgct gatagctgtc cagggcctca
     6001 ttcagggcat ctacggcaac aaatggtgac aatgtctgcg ccagacgctg taaattccac
     6061 aacgcgacag caggttgatt atcaaaacta taacgccctt gatgatccga gtgattacaa
     6121 ataaaaccgg gttcgtaatc atcaagaaaa ccaaacggcc cgtaatcaag cgtcagtccc
     6181 agcagcgaca tgttgtcggt attcatcacc ccatgtgcaa agccgaccgt ctgccattgg
     6241 gcaattaacg acgcggtacg tgcgacaaca tcgctaaacc agagacggta tttgtcctca
     6301 tcatcttcaa gatgtgacca gtaatgacga atagcaaagt cagctaactg acgaaccttt
     6361 tccgactcgc ggcggtagta aaaatgttcg aaatgaccaa agcgcagatg acttggtgcc
     6421 acacgcatca gcatcgcgcc tggctccgcc gtttcccgat acactggcga atcgctggtg
     6481 acgatactta acgcgcgggt cgtcggaatg cccagataat gcatcgcctc actggcgaga
     6541 ctttctcgta tcgtcgaacg taaaaccgcc cgtccatcac ccattcgcga ataaggcgtc
     6601 aggccagcac ctttcagatg ccagtccatt gtagtgccat cagcaagcag ttgttcgccg
     6661 agtaaaatgc cgcgcccatc acccagttgg cccgcccaga cgccgaactg atgaccactg
     6721 taaacctgcg ccagtggtga cataccaggc agtagggctt cgccgcccca gacacctgcg
     6781 ccatttttaa acagcgacga tggaatactc agcgtgttag ccagttcggt attatgccaa
     6841 atcagccggg cattatttaa aggcgtaggg gaaagtgctg tataggtttc tggcaattca
     6901 tcgcgccagc gggtaacaaa agacagggtc atagatcctc ctgtttgata gtgtagacgg
     6961 ttactctcgt cttaaacacc agcaaacaga agggttatcg ctgaaccaac gtcgttatct
     7021 gatttatggg aacggcaggc cacaggcatc cctggagagc atggaaatcg aagggggtaa
     7081 tctgtgccaa tatttctgcc gtatcaatgc ctcccgcaat gatgcaattg caacaagggg
     7141 aaatttgcgc ctggatcgca cgaataaacg gttcgaagga gcggtgcgtg atttgttgct
     7201 gaataaaact tttatccagc atgacgcggg tgaaaagacc atcaaaaacc gctttcattg
     7261 tgctattgcc tgctcccaaa ttacctaaca caaggggata cacttgcgat aaggataaaa
     7321 gaccgcgatt atcttttcct tcattgagat gcgggtaatt ctcattaatc aacagttcaa
     7381 taaacggata ttttaatagt tcgcctgcgt aattatccct ctctaataat aatgtagcca
     7441 cttgtggcgt taaattaagc caggcaaaga gtttatgttg aataaaaaaa tgttggcatg
     7501 attttagtaa ttccaactgc tctgaaaata attgccaatg ttgctcctcg gtaagttgcg
     7561 ctataacccg actcgtcggg atacgcaccg tgccatcttc actggagaaa tgggtaatta
     7621 actcaacgcc gacaaggact tgctggttat ctcttatcgg caggaaatag caatcagaat
     7681 gataaagatt ctccaaaaaa atcttcatgg taaccgtccc tctcgaagga tgtttttcag
     7741 tatccggcta aaagggatga ggccataaga cataacaatt acagaaggag taactttcat
     7801 ttgttccatg ttaaccactt tttcaggggt ccttttttta gattatcctg attataaacg
     7861 aataatccat ttacggaatt
//
LOCUS       DAZPLM010000194         7824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPLM010000194 DAZPLM010000000
VERSION     DAZPLM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.194"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..904)
                     /gene="nac"
                     /locus_tag="ACHVC7_003515"
     CDS             complement(<1..904)
                     /gene="nac"
                     /locus_tag="ACHVC7_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR2117472.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1231..1306
                     /locus_tag="ACHVC7_003516"
     tRNA            1231..1306
                     /locus_tag="ACHVC7_003516"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1264..1266,aa:Asn,seq:gtt)
     gene            complement(1362..2297)
                     /gene="ldtA"
                     /locus_tag="ACHVC7_003517"
     CDS             complement(1362..2297)
                     /gene="ldtA"
                     /locus_tag="ACHVC7_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2117473.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHVC7_003518"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHVC7_003518"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR2117474.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHVC7_003519"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHVC7_003519"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR2117475.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHVC7_003520"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHVC7_003520"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR2117476.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHVC7_003521"
     CDS             complement(4869..5306)
                     /locus_tag="ACHVC7_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR2117477.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHVC7_003522"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHVC7_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHVC7_003523"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHVC7_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR2117478.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2192 a         2077 c         1666 g         1889 t
ORIGIN      
        1 gccactgcct tttttccatc actggggaac taatcactga cattaacaac tctttcactg
       61 cctgtgcctg tggcgataag ttcgctctgg cgggtaaatt taatgacaaa gagagactca
      121 tggaaggagt ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcgg
      181 ccgattcggg taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa
      241 tagactcaat ttcaccaatg acttttgccg tgaatcgccg taaggaaaaa gcctcatcaa
      301 cacgaagtct aatagcactg taatcactgg ggagaaagag gttcattttc gcaatagcat
      361 tcacatcaac gctttgcccc gggcaatctt gagttcctac cagaaaaaga tcttctttca
      421 gcaaagcctg actggataca ccagccacag gggaatgctc ataaatcacc gccatatcga
      481 gttggtgatt tatcaatttt tcgttaagca ctgcaccact attttcatga agatagataa
      541 cgatctccgg aaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag
      601 cggttcctgg tgcaaagcca atcgagactt gccccgataa tgcctgacca acgttatgca
      661 ccgccagttg ggcctgttca cactgacgta aaatggcccg cgcatgggta tagagaattt
      721 ttccggcgtc tgttggcgta acgccccgct ttgtacggat caaaagttgt tgatttaact
      781 caccttccag tgtggcaacc tgctggctga gcgctggttg tgcgatatgc aatacttcag
      841 cagcctgggt caggctacca atatctacaa tttttacgaa gtatttcagg cgtctgaagt
      901 tcatgttgcc tccggttttt aagaatcggc ccaagtgccg ccattactta caaccagatt
      961 gcaagatgct tgccagtttt attttggcgt tgatgtacaa gctaaccaac tgtcaaataa
     1021 gagattatga tagattcgtc atttgctcct ttaatcagct gtcgcgttcc cctgccctat
     1081 aaaaggaggg tatgcaccac gatggttcat tacccaataa gattgaaagc tcaccacttt
     1141 gttgaaattg acagcaaaca aacaaaaaaa tgcatttcac cctttgacat caccatgcac
     1201 tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg
     1261 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc
     1321 tgctttctag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     1381 ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt tgcgcgattt
     1441 acatcaactt cttgcccgct gataaacgcc cgcaaagatg gggttaccgg caatggcact
     1501 tttcggtcag actcatattc tgcacgattg cgcgacaacg gctcatgaac ttccagccag
     1561 ttcgagccat ctggttcagt ggtgtatttt actggctggt cgataatttg cacacgcgtc
     1621 ccaacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     1681 tgacttaccc ggagcccaat accaaaattg gcattggtac catggatggc atacaacctg
     1741 ccaatataaa tcgcgtacag ccccatggga ttatcggggc ccgcaggaac aaatgcgggc
     1801 aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt ccaggttgga
     1861 gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg
     1921 ccgataccaa taggaaagac ttccacagta ttactgtctg gtgggtagta ataaagacgc
     1981 atctcagcga cgttaaccac aatcccttta cgaacagtgg cgggcaaaat cagttgctgc
     2041 ggaatggtga gttgcgagcc agacttcggc aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaa
//
LOCUS       DAZPLM010000195         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPLM010000195 DAZPLM010000000
VERSION     DAZPLM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.195"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHVC7_003524"
     CDS             complement(<1..1780)
                     /locus_tag="ACHVC7_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR2117479.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHVC7_003525"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHVC7_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR2117480.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHVC7_003526"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHVC7_003526"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR2117481.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHVC7_003527"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHVC7_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR2117482.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHVC7_003528"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHVC7_003528"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR2117483.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHVC7_003529"
     CDS             complement(5968..7005)
                     /locus_tag="ACHVC7_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR2117484.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHVC7_003530"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHVC7_003530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR2117485.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZPLM010000196         7687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPLM010000196 DAZPLM010000000
VERSION     DAZPLM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.196"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            150..653
                     /gene="dps"
                     /locus_tag="ACHVC7_003531"
                     /gene_synonym="pexB"
     CDS             150..653
                     /gene="dps"
                     /locus_tag="ACHVC7_003531"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR2117486.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1057..1803
                     /gene="glnH"
                     /locus_tag="ACHVC7_003532"
     CDS             1057..1803
                     /gene="glnH"
                     /locus_tag="ACHVC7_003532"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR2117487.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1942..2601
                     /gene="glnP"
                     /locus_tag="ACHVC7_003533"
     CDS             1942..2601
                     /gene="glnP"
                     /locus_tag="ACHVC7_003533"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR2117488.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2598..3320
                     /gene="glnQ"
                     /locus_tag="ACHVC7_003534"
     CDS             2598..3320
                     /gene="glnQ"
                     /locus_tag="ACHVC7_003534"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR2117489.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3437..5662
                     /gene="ybiO"
                     /locus_tag="ACHVC7_003535"
     CDS             3437..5662
                     /gene="ybiO"
                     /locus_tag="ACHVC7_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR2117490.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5659..6585)
                     /gene="rlmF"
                     /locus_tag="ACHVC7_003536"
     CDS             complement(5659..6585)
                     /gene="rlmF"
                     /locus_tag="ACHVC7_003536"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR2117491.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6861..7121
                     /gene="mcbA"
                     /locus_tag="ACHVC7_003537"
     CDS             6861..7121
                     /gene="mcbA"
                     /locus_tag="ACHVC7_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR2117492.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7386..>7687
                     /locus_tag="ACHVC7_003538"
     CDS             7386..>7687
                     /locus_tag="ACHVC7_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR2117493.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPGV"
BASE COUNT         1858 a         1967 c         1935 g         1927 t
ORIGIN      
        1 tctaactcat tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga
       61 taggaacagc cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact
      121 gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag
      181 cgaccaatct gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag
      241 agttgctgaa tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc
      301 actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca
      361 ccgcactgat cgatcatctg gataccatgg cagaacgtgc agtgcagctg ggcggtgtag
      421 ctctggggac cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca
      481 tccacaacgt tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg
      541 acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg
      601 cgtctcgcga cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc
      661 gctgatggtg cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg
      721 ttttgcagcc cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta
      781 agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca
      841 ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc
      901 ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat
      961 ctcgaaaaat caaggagttg caaaactggc acgatttttt catatatgtg aatgtcacgc
     1021 aggggatcgt cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca
     1081 ctggctgcac tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt
     1141 gtcgcgacgg ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc
     1201 tttgacgttg atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag
     1261 ccgatggatt tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg
     1321 gcgggcatta ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac
     1381 aaaagcggcc tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat
     1441 ctcgacggga aagtggttgc tgttaagagc ggtactggct ccgttgatta cgcgaaagca
     1501 aacatcaaaa ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg
     1561 ggcaccaacc gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa
     1621 accgccggta acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt
     1681 attgcgttcc cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc
     1741 ctgcgcgaga acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa
     1801 taataacgct acacctgtaa agcgcactgg cagttccctc tcccctctgg ggagaggatt
     1861 agggtgaggg gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca
     1921 ccacggtaac aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg
     1981 cttctgattg aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt
     2041 ctggtaatcg gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac
     2101 gtcgcgctgg tctttattga ggtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt
     2161 atttatttcg ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg
     2221 gtggtcacca tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg
     2281 ctgtctattc acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa
     2341 accattcgct acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac
     2401 cagtggatca tcagcattaa agacacctcg ctgtttattg tgatcggcgt ggcggaactg
     2461 acccgtcagg ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc
     2521 gtggcggtgt tctatctgat tattaccctg gtgctaagct ttattttgcg tcgtctggaa
     2581 agaaggatga aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc
     2641 aggtgcttca caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc
     2701 cgtccggttc cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct
     2761 ccggcgatct gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga
     2821 ttcgccagga agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc
     2881 tggaaaacgt catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa
     2941 aactggcgcg tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt
     3001 ccgaactttc tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc
     3061 cgaaaatgat gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag
     3121 tgctgaaggt tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg
     3181 aaatcggttt tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg
     3241 cggaagatgg cgatccgcag gtattgatca agaacccgcc gagccagcgc ttgcaggaat
     3301 ttttgcagca cgtctcttaa cttctctgcc tccctttcac ccgaaaggga ggcacaccag
     3361 attcctctca tttaaaatcg cccctccccc agcatctata cttatctttt tgctctgttt
     3421 tctcactgga ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc
     3481 tgcccacgcg gtatccatac ccggcgttac aaccacaacg acaacggact caacgactga
     3541 accggccccg gaaccggata tcgaacaaaa aaaagcggcc tatggcgcac tggcggatgt
     3601 gctggataac gacacctcgc gtaaagaatt gatcgaccag ttgcgtactg tcgccgcgac
     3661 gccccctgcg gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt
     3721 gctgcaaaag gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg
     3781 gcaactttat cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag
     3841 caatgcgctg agccattttt caatgttagc ggtattagtg tttggttttt actggctgat
     3901 tcgcctgtgc gcactgccgc tgtatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg
     3961 tgagcgcagc aactggttgc agcttccggc gatgattatc ggggcgttta ttatcgacct
     4021 gctgttactg gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg
     4081 cagtcgcacc atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt
     4141 tttcaaagcc gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac
     4201 gattcaggac gagagcgccc gttactggag tcgtcgcctg agctggttaa gcagtttgat
     4261 aggttatggc ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg
     4321 tgcgctggcg aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt
     4381 tcgtaataaa aaagagatta cccagcattt gctcaacttt gcggagcatt cgctggcctt
     4441 tttcagcctg tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt
     4501 tatcgtgctg tttttctttt cgttgttcga tccgggcaac agcctgaaat ttatgatggg
     4561 tgcaacggtg cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc
     4621 ccgctggctg gcaaaaacca tcactctctc gccacatact cagcgtaact atccagagtt
     4681 gcaaaaacgg ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt
     4741 ctgcgtggcg gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct
     4801 gcaaaacggc gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac ttattctttt
     4861 tttctcggcg gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga
     4921 tattcatggc cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa
     4981 cgcgctggcg gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt
     5041 caatatcgcg ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc
     5101 gcaaacgctg gtgaaagata tcattaccgg ggtgtttatt cagtttgaaa acggcatgaa
     5161 cactggagat ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg
     5221 ctccgtgggc gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac
     5281 cacctttgct aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg
     5341 ccatgaagat gctgataaag ccaatcaggc actgaaagat gcggtagcgg aattaatgga
     5401 aaacgaagaa attcgcgggc tgattattgg tgaaccgaat tttgccggga ttgtcggctt
     5461 aagcaatacc gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac
     5521 ggtacgcttt gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc
     5581 gccagtgcag acttatcagg tgctgcctac tccgggcgcg accccggctg aaccgttacc
     5641 gccgggggag ccaacgcttt aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt
     5701 cataaaggtc caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac
     5761 caccttcacc gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg
     5821 agataccagc gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat
     5881 ctttttgata aaggcgactt caccaccttc acaccacaac tcctgttgct gaccaccaaa
     5941 gttcagtgca tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg
     6001 cgctgcagcg gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt
     6061 tttatggatg atgccgttaa aaattgcccc actctctttt tgccgacgca gacgaatggc
     6121 gcggttaagc cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct
     6181 ggtttcacta ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca
     6241 gttcgcacca acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc
     6301 agccagtaaa tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag
     6361 aaaaccgtcg gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt
     6421 gagcgccttc accgccagcg gattggcaaa gtctacgctt tgctccccgg cgggtgtaag
     6481 cgtgaggaat tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct
     6541 gtgatgacgg ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa
     6601 gcagcggcgt aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt
     6661 ccccaaaacc ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc
     6721 atgtcattaa tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag cacatctctg
     6781 tcaatttttt gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt
     6841 gtatttatga ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga
     6901 gcggaatctc tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc
     6961 tacggcccgc cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata
     7021 aactggcaga aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg
     7081 gaaatgacca gatgttcggt actgcaacca tctacaaata accccaccaa cgtaactgca
     7141 ttcgcctctg gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaactca
     7201 atgtaaataa atgtatttct ttttcgcgca atgggtgata gaaaatcgcg ccaagtgata
     7261 atgcttatca aaattattat cacttgcacg aacactatca cgggattaac agtggcatcg
     7321 catccgcaga gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct
     7381 gcaaaatgga aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg
     7441 ccggtctttg tattggcatc acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta
     7501 acgcggatga cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat
     7561 ctgccgatcc gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt
     7621 ctgaacaagt gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgccc
     7681 ccggcgt
//
LOCUS       DAZPLM010000197         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPLM010000197 DAZPLM010000000
VERSION     DAZPLM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.197"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHVC7_003539"
     CDS             171..701
                     /locus_tag="ACHVC7_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR2117494.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHVC7_003540"
     CDS             837..1502
                     /locus_tag="ACHVC7_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117495.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHVC7_003541"
     CDS             1824..2447
                     /locus_tag="ACHVC7_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR2117496.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHVC7_003542"
     CDS             2532..2750
                     /locus_tag="ACHVC7_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR2117497.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHVC7_003543"
     CDS             2728..3798
                     /locus_tag="ACHVC7_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2117498.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHVC7_003544"
     CDS             3933..5216
                     /locus_tag="ACHVC7_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR2117499.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHVC7_003545"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHVC7_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR2117500.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZPLM010000198         7604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPLM010000198 DAZPLM010000000
VERSION     DAZPLM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.198"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            93..1382
                     /gene="hpaG"
                     /locus_tag="ACHVC7_003546"
     CDS             93..1382
                     /gene="hpaG"
                     /locus_tag="ACHVC7_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR2117501.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1379..2845
                     /gene="hpaE"
                     /locus_tag="ACHVC7_003547"
     CDS             1379..2845
                     /gene="hpaE"
                     /locus_tag="ACHVC7_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2117502.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2847..3698
                     /gene="hpaD"
                     /locus_tag="ACHVC7_003548"
     CDS             2847..3698
                     /gene="hpaD"
                     /locus_tag="ACHVC7_003548"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR2117503.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3708..4088
                     /locus_tag="ACHVC7_003549"
     CDS             3708..4088
                     /locus_tag="ACHVC7_003549"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR2117504.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4199..5002
                     /gene="hpaH"
                     /locus_tag="ACHVC7_003550"
     CDS             4199..5002
                     /gene="hpaH"
                     /locus_tag="ACHVC7_003550"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR2117505.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5013..5801
                     /gene="hpaI"
                     /locus_tag="ACHVC7_003551"
     CDS             5013..5801
                     /gene="hpaI"
                     /locus_tag="ACHVC7_003551"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR2117506.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5976..7352
                     /gene="hpaX"
                     /locus_tag="ACHVC7_003552"
     CDS             5976..7352
                     /gene="hpaX"
                     /locus_tag="ACHVC7_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR2117507.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7362..>7604
                     /locus_tag="ACHVC7_003553"
     CDS             7362..>7604
                     /locus_tag="ACHVC7_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR2117508.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1748 a         2133 c         2152 g         1571 t
ORIGIN      
        1 tacatcactt acttttgcca aactacgcaa tcgggaataa aagcaatcat taacatatta
       61 atgattaaga tgatcgaatc cgaggagtgg taatgaaagg cactatcttc gccgtagcgt
      121 tgaaccatcg cagccaactt gatgcatggc aggaagcgtt ccagcaatcc ccctacaaag
      181 ccccgcctaa aactgcggtc tggtttatta aaccgcgcaa tacggtgatt ggttgcggtg
      241 aaccgattcc ctttccacag ggtgaaaagg tactgagcgg tgcgactgtt gcgctgattg
      301 tgggaaaaac ggcgacgaaa gtacgtgaag aagatgcggc agagtacatc gccggatatg
      361 cgctggctaa cgacgtcagc ctgccggaag agagctttta ccgcccggca atcaaagcaa
      421 aatgtcgtga tggattctgc cccattggcg aaaccgtggc tctcagcaat gtcgataatc
      481 tgaccatcta taccgagatc aacgggcgtc ctgccgatca ctggaatacc gccgatttac
      541 aacgtaacgc cgcgcagttg ctgagcgccc tgagcgaatt tgccacgctg aatccaggcg
      601 acgccattct gctcggcacg ccacaggcgc gcgtggaaat acagccaggc gatcgcgttc
      661 gtgttctcgc agaaggtttc ccgccgctgg aaaatccggt agtggacgaa cgtgaagtga
      721 ccacgcacaa gagcttccca acgcagccac acccgcacgg cacgctgttt gccctcggcc
      781 tgaactacgc cgaccacgcc agcgaactgg aatttaagcc accggaagaa ccgctggtgt
      841 tcctgaaagc gccaaatacc ctcactggcg ataaccagac ctccgtgcgt ccaaacaata
      901 ttgaatacat gcactatgaa gcggagctgg tggtagttat tggcaaacag gcgcgtaacg
      961 tcagcgaagc cgatgccatg gattatgtcg cgggctacac cgtgtgtaac gactacgcca
     1021 ttcgtgacta tctggaaaac tactaccgcc ctaacctgcg ggtaaaaagc cgcgacggac
     1081 tgacgccgat gctttcaacc atcgtgccga aagaggcgat cccggacccg cataatctga
     1141 cccttcgcac cttcgtcaac ggcgagttac gccagcaagg caccaccgcc gatctgatct
     1201 tcagcgtgcc cttcctgatc gcctacttaa gtgaatttat gaccctgaat ccgggcgaca
     1261 tgatcgccac cggcacaccc aaaggcttat ctgacgtggt gcctggcgat gaagtagtgg
     1321 tggaagtaga aggcgtgggc cgcctggtga accgaattgt gagtgaggat acagcgaaat
     1381 gaaaaaagta aatcattgga tcaacggcaa aaatgttgca ggtaacgact acttccagac
     1441 caccaatccg gcaacgggtg aagtgctggc ggatgtggcc tctggcggtg aagcggagat
     1501 caatcaggcg gtagcggcag cgaaagaggc gttcccgaaa tgggccaatc tgccgatgaa
     1561 agagcgtgcg cgcctgatgc gccgtctggg cgatctgatc gaccagaacg tgccagagat
     1621 cgccgcgatg gaaaccgcgg acaccggcct gccgatccat cagaccaaaa atgtgttgat
     1681 cccacgcgct tcccacaact ttgaattttt cgcggaagtc tgccagcaga tgaacggcaa
     1741 gacctatccg gttgacgaca agatgctcaa ctacacgctg gtgcagccgg tgggcgtttg
     1801 tgcgctggta tcgccgtgga acgtaccgtt tatgaccgcc acatggaagg tcgcgccgtg
     1861 tctggcgctg ggcaataccg cggtactgaa aatgtcggaa ctctccccgc tgaccgctga
     1921 cagcctgggt gagctggcgc tggaagccga tattccggca ggcgtgctga acgtggtaca
     1981 gggctacggc gcaaccgcag gggatgcgct ggttcgtcat catgacgtac gtgccgtgtc
     2041 gttcaccggc ggtacggcca ccgggcgcaa catcatgaaa aacgccgggc tgaaaaaata
     2101 ctccatggaa ctgggcggta aatcgccggt gctgattttt gaagatgccg atattgaacg
     2161 cgcgctggac gccgccctgt tcaccatctt ctcgatcaac ggcgaacgct gcaccgccgg
     2221 ttcgcgcatc tttattcagc aaagcatcta cccggaattc gttaaacgct ttgccgaacg
     2281 cgccaaccgt ctgcgcgtgg gcgatccgaa cgatccgaat acccaggttg gcgcgcttat
     2341 tagccagcag cactgggaaa aagtctccgg ctatatccgt ctcggcattg aagaaggcgc
     2401 aaccctgctg gcgggcggcc cggataaacc gtccgacctg cctgcgcacc tgaaaggcgg
     2461 caacttcctg cgcccaaccg tgctggcaga cgttgataac cgtatgcgag tcgcccagga
     2521 agagattttc gggccggtcg cctgcctgct gccgtttaaa gacgaagccg aaggcttacg
     2581 tctggcaaac gacgtggagt acggcctcgc gtcgtacatc tggacacagg atgtcagcaa
     2641 agtgttacgc ctggcgcgtg gcattgaagc tggcatggtg ttcgtcaaca cccagaacgt
     2701 gcgtgacctg cgccagccat ttggcggcgt aaaagcctcc ggcaccgggc gtgaaggcgg
     2761 tgagtacagc ttcgaagtgt tcgcggaaat gaagaacgtc tgcatttcca tgggcgacca
     2821 tccaattccg aaatggggag tctgatatgg gtaagttagc gttagccgcc aagatcacgc
     2881 acgtaccgtc gatgtatcac tctgaactgc cagggaaaaa ccacggttgc cgccagggcg
     2941 cgatcgacgg gcataaagag atcagcaagc gttgccggga aatgggcgtc gataccatta
     3001 tcgttttcga tacccactgg ctggtcaaca gtgcttatca catcaactgt gcagaccatt
     3061 ttgaaggcgt ctacaccagc aacgagctgc cgcattttat ccgcgatatg acctacaact
     3121 acgagggcaa cccggagttg gggcagctta ttgccgatga agccttaaag ctcggcgtgc
     3181 gggcaaaagc gcacaacatt cccagcctga aactggaata cggcacgctg gtgccgatgc
     3241 gctacatgaa tgaagacaag cacttcaaag tggtctccat ttcggctttc tgcacggttc
     3301 acgattttgc cgacagccgc aagctgggcg aagcgattct gaaagcgatc gaacagtacg
     3361 acggcaccgt ggcggtcctt gccagcggtt cgttatcgca ccgctttatt gacgatcagc
     3421 gtgcggaaga agggatgaac agctacaccc gcgagttcga ccgccagatg gacgagcgcg
     3481 tggtgaagtt gtggcgcgaa ggccagttca aagagttctg caatatgctg ccggagtacg
     3541 ccgactactg ctacggcgaa ggcaatatgc acgacacggt gatgctgctg gggatgctcg
     3601 gctgggataa atacgacggc aaggtggagt ttatcaccga gctattccca agctctggca
     3661 ccggtcaggt taacgctgtt ttcccgcttc ccgcgtaagg acgttttatg ccgcacttta
     3721 tcgttaaatg cagtgacaac atccgcgaag aagccgacct gccggggttg ttcgccaaag
     3781 tgaatccgac gctggcagcc acgggtattt ttccgctggc gggtattcgc agccgcgtgc
     3841 attgggtcga tacctggcag atggccgacg ggcagcgtga ttatgccttc gtgcatatga
     3901 cgttgaaaat cggcgcaggt cgcagcctgg aaagccgcca gcaggcgggt gaaatgctgt
     3961 ttgaactgat taaaacgcac ttcgccgccc tgatggagag ccgcctgctg gcgttgtcgt
     4021 ttgagattga agagctgcat ccgacgctga attttaaaca aaacaacgtg cacgcattgt
     4081 ttaagtagcg cgcagattgc ccggtggcgc tgcgcttacc gggcctacaa aaccccaaac
     4141 cgtacaccgt aggccggata aggcgcagcc gcatccggca atgccacagg atatcgctat
     4201 gttcgataaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg
     4261 cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag acgcttacgc
     4321 ggtgcagcgt gaatgggttc gcctgaaaat cgccgaaggt cgcacgctga aaggccacaa
     4381 aatcggcctg acctcgaaag cgatgcaggc aagctcgcag atcagcgaac cggattacgg
     4441 cgcgctgctg gatgacatgt tcttccacga tggcagcgat atcccgaccg atcgctttat
     4501 cgtgccgcgc attgaagtgg agctggcttt cgtgctggca aaaccgctgc gtgggccaaa
     4561 ctgcacgctg ttcgacgtct acaacgccac ggactacgtg atcccggcgc tggagctgat
     4621 cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgcaaag tgttcgacac
     4681 catttctgat aacgccgcca acgccggggt gatcctcggc ggtcgtccga tcaaaccaga
     4741 cgaactggat ctgcgttgga tctccgccct gatgtatcgc aatggcgtga ttgaagaaac
     4801 cggcgtcgcc gctggcgtgc tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa
     4861 actcgcaccg tatgacgtac aactggaagc cgggcaaatc attctcggtg gttcgttcac
     4921 ccgcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca acatgggttc
     4981 cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagctttaaa gcggcgctga
     5041 aagcaggccg tccgcagatc ggattatggc tggggctgag tagcagctac agcgcagagt
     5101 tactggccgg agcaggattc gactggttgt tgatcgacgg tgagcacacg ccgaataacg
     5161 tgcaaaccgt gctcacccag ctacaggcga ttgcgcccta ccccagccag ccggtagtac
     5221 gtccgtcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtcggc acacaaacct
     5281 tgctggtgcc gatggtacaa aacgctgacg aagcccgtga agcggtacgc gccacccgtt
     5341 atccccccgc cggtattcgc ggtgtgggca gtgcgctggc ccgcgcctcg cgctggaatc
     5401 gtattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa
     5461 cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggcgtc gacggcgtgt
     5521 ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg cagcacccgg
     5581 aagtacaggc cgccattgag caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg
     5641 ggatcctgat cgccaatgag caactggcaa aacgctatct ggaactgggc gcgctgtttg
     5701 tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc cgaagcgctg gcagcacggt
     5761 ttggtgcgca ggccaccgcc gtgaagcccg gcgtgtatta atgccggatg cgctgcgctt
     5821 atccggccta cactcgcacc aaaccgtagg ccggataagg cgtttacgcc gcatccggca
     5881 aaaagccgta ccaaatcgcg gaccggataa ggcgttacgc cgcatccgcc ataaaccttg
     5941 tcgtacccta caaaaatccc attagaggaa gaaaaatgag cgacacctca cctgccatac
     6001 cggagagtat cgatccggcg aatcagcata aagcgctgac tgccggacaa caggcggtta
     6061 ttaagaagct atttcgccgc ctgagcgtct ttctgttcgt gctgtttatc ttctcgttcc
     6121 ttgatcgcat caacatcggc tttgccggac tcacgatggg acgcgacctc ggtctgagcg
     6181 ccaccatgtt tggcctcgct accaccctgt tctatgccgc ttatgtcatc ttcggcattc
     6241 ccagcaacat tatgctgagt attgtcggtg cgcggcgctg gatcgccacc atcatggtgc
     6301 tctggggcat cgcctctact gccaccatgt ttgccactgg ccccaccagc ttgtacgtac
     6361 tgcgtatact ggttggcatt accgaagccg gctttctgcc tggtattctg ctgtatttaa
     6421 ccttctggtt tccggcctat ttccgcgccc gtgccaacgc cttgtttatg gtagcaatgc
     6481 cggtaacgac agcgttgggc tcgatcgttt ccggctacat tttgtcgctg gatggcgtca
     6541 tggcattaaa aggctggcag tggctgtttt tgctggaagg cttcccgtcg gtattactcg
     6601 gcgtcatggt gtggttctgg cttgatgact caccggacaa agctaagtgg ctgacgaaag
     6661 aagacaaaaa atgcctgcaa gagatgatgg ataacgatcg tctgacgctg gttcagccag
     6721 agggagccat cagccaccac gccatgcaac aacgcagtat gtggcgggag atcttcactc
     6781 cggtggtgat gatgtatacc ctggcgtatt tctgcctgac caacacactt agtgcgatca
     6841 gcatctggac accgcagatc ctgcaaagtt ttaatcaggg cagcagtaat atcaccatcg
     6901 gcatgctggc cgccgtaccg cagatttgta ccattctcgg gatggtctac tggagccgtc
     6961 actcagatcg ccgccaggaa cgaaggcatc acaccgccct tccttatttg ttcgctgccg
     7021 caggttggtt actggcttcg gcaactgatc acaacatgat ccagatgctg gggatcatta
     7081 tggcttcgac cggatcattc agcgcaatgg cgattttctg gacaacaccg gatcagtcca
     7141 tcagcctgcg ggcacgagcg atcggtattg cggtgatcaa cgccactggc aacattggtt
     7201 cagcgttaag tccgtttatg atcggctggt tgaaagattt gaccggcagc tttaacagtg
     7261 gattgtggtt tgttgccgcg ctgctggtga ttggtgcggg gattatctgg gcgattccaa
     7321 tgcagtcctc ccgtccgcga gcgaccctgt aaggaacgac gatgtgtgac cgtcagattg
     7381 ccaatattga tatcagcaaa gagtacgatg aaagcctggg cacggacgat gtgcattatc
     7441 agtcgttcgc ccgcatggcg gcattttttg gccgccatat gctgccacat cgccacgaac
     7501 agtactttca gatgcatttc ctcaatagcg gacagattga gctacagctt gacgatcatc
     7561 gctactcggt ggaagcgccc ctgtttgtcc tgacgccgcc gtca
//
LOCUS       DAZPLM010000199         7568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPLM010000199 DAZPLM010000000
VERSION     DAZPLM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.199"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(38..414)
                     /gene="rnpB"
                     /locus_tag="ACHVC7_003554"
     ncRNA           complement(38..414)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHVC7_003554"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHVC7_003555"
     CDS             complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHVC7_003555"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR2117509.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHVC7_003556"
     CDS             complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHVC7_003556"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR2117510.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHVC7_003557"
     CDS             complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHVC7_003557"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR2117511.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHVC7_003558"
     CDS             complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHVC7_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR2117512.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5104..6675
                     /gene="garD"
                     /locus_tag="ACHVC7_003559"
     CDS             5104..6675
                     /gene="garD"
                     /locus_tag="ACHVC7_003559"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR2117513.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6824..7236
                     /locus_tag="ACHVC7_003560"
                     /pseudo
     CDS             6824..7236
                     /locus_tag="ACHVC7_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            7236..>7568
                     /gene="yhaV"
                     /locus_tag="ACHVC7_003561"
     CDS             7236..>7568
                     /gene="yhaV"
                     /locus_tag="ACHVC7_003561"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR2117514.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWM"
BASE COUNT         1910 a         2029 c         1844 g         1785 t
ORIGIN      
        1 aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg
       61 ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct
      121 acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag
      181 tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc
      241 ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg
      301 tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc
      361 gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg
      421 cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc
      481 gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc
      541 aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg atgtacaacg
      601 ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc
      661 acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca
      721 ccggtgatca ccagcgtaca atcgtgaata tgttcttcca gattcagcgc cgtagtgacg
      781 atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccataccg
      841 cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca
      901 tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt
      961 tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa
     1021 gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc
     1081 agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc
     1141 gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg
     1201 ataataatgt ttgttgcact gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt
     1261 gaagtggtta cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc
     1321 gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc
     1381 tcgcccagcg gccctgtaac ccaggcgtga cgttcagccc cctgggtggc tgcaatcatc
     1441 gcttccaccg ttccttcacc accgtcggca accggaacag aaacatactg tgcatcagga
     1501 aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct
     1561 ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg
     1621 agggcgtttc tcttattcgg cctagattcc aggcccggat tgcaatacac catccgggca
     1681 cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg
     1741 ctatgatccg ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca
     1801 gctgtgagca gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc
     1861 ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac catcggcgct
     1921 ttggcatcca gcacggtact gcccgccagc ccaccgcgaa ttgcctgata aaccaggtcc
     1981 gggttaacgc ccgctttggt tgccagcgtt aacgcttctg acatcgcagc aatattcagc
     2041 gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc
     2101 accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg
     2161 ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga
     2221 gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc
     2281 ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca
     2341 ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg
     2401 tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc agcaatcacg
     2461 tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg
     2521 tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata
     2581 tcaaccatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag
     2641 tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg ccccattcca
     2701 gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcgc
     2761 tggcacggtt aaaaatatgc tgaattgctt tttgtacatc cggatgcgag gcattgccga
     2821 gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acaccttcgg
     2881 tagcggcaat agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag
     2941 taatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata ttggcgcggt
     3001 gagaaacgga gacgccgcga atgccttccg gcgggtaacg agttgatgcc accgcctgct
     3061 ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa
     3121 gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc
     3181 ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca tgttcgccat
     3241 ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga
     3301 gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg
     3361 ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa
     3421 tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa
     3481 ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga
     3541 caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt
     3601 tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag
     3661 ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg
     3721 tggaaaccag caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac
     3781 cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca
     3841 gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca
     3901 gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga
     3961 aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac
     4021 cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag
     4081 agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact
     4141 tgatccacag cgccgtcagc acaaaaccaa tcccccccat aacggtgaag acgtgctccc
     4201 agccccaagc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt
     4261 gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga
     4321 caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata aagcgcataa
     4381 agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg
     4441 tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa
     4501 gcaaccagcc gccggggatt tgcatcagca agtaggccca gccaaaagca gagaagatgt
     4561 aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca
     4621 gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat
     4681 agctggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct
     4741 cgggtactta tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat
     4801 gccgttacga caatgaactc gggaattagt ataagcagcg ggagaataat aatcattgtg
     4861 caaatgctaa tttaatcagt actatttaaa tattattttg agcatatgca cataaggttg
     4921 cgcgctaaag cacagatttg cgctttacct taccgggcgg cactgcaatc cctgaaatga
     4981 ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt
     5041 catttatttt taaatgagac cagatcctca ttttaataac ccctggctgg agaatattgc
     5101 acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac
     5161 gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt
     5221 ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac
     5281 attccagcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgcgca
     5341 atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta
     5401 cacacgctgc cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc
     5461 tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc
     5521 accagcgtcc actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat
     5581 ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt
     5641 ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg
     5701 ctgaacccta actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag
     5761 cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc
     5821 attgtcagtt tgcaggatga aaagcatgtc ggctttcagt ccatggtcga ggatattttg
     5881 caggtcgccg aacgccatct acaaaaactg aatcaacggc agcgagaaac ctgcccggct
     5941 tcagaactgg tcgttggtgt gcagtgcggt ggcagcgatg cgttttctgg tgtaacggca
     6001 aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt
     6061 tcagaagtaa cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa
     6121 gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga
     6181 aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaatgtg
     6241 gtggagaagg cactcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg
     6301 tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat
     6361 tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tacaagtgtt tacgaccggc
     6421 cgtggtacgc cgtacggcct gatggcggta cccgtaatta aaatggcaac ccgcaccgag
     6481 ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc taccggcgaa
     6541 gagagcattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg
     6601 aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcggt gtttaacccg
     6661 gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt
     6721 ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc
     6781 ttataatttg agcagaaaac aggctgtaaa aggacaatga atcatgcccg ctaatgctcg
     6841 ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc
     6901 cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat
     6961 tctgcctggt gggcaagtat ttatgtgccg actgggagat gaacaggagg atcatactat
     7021 gaatgcattt ttgcgttttc tggatacaga tatccagaac aacccgcaaa aaactcgtcc
     7081 attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca ttcaacattc
     7141 aacattcaac attcaacatt caacattcaa caaggaaaga aacttgtcgc tggcatggac
     7201 gtcaacattg atgatgagat tggcgacgac gaataatgga ttttccacaa agggttaatg
     7261 gttgggcgct atatgctcat ccctgttttc aggaaaccta cgacgcttta gttgccgaag
     7321 tcgagacatt aaagggaaaa gatcctgaaa attatcagag aaaagccgcc acaaagttat
     7381 tggcggtagt ccataaagtg attgaggagc atatcacggt caatccatca tcaccggcat
     7441 tccgtcatgg caagtcgtta ggctctggga aaaataaaga ctggtcacgg gtaaaatttg
     7501 gtgcaggtcg ttatcgtctc ttctttcgtt atagtgaaaa agagaaagtc atcattctgg
     7561 gatggatg
//
LOCUS       DAZPLM010000200         7520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPLM010000200 DAZPLM010000000
VERSION     DAZPLM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.200"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHVC7_003562"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHVC7_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR2117515.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHVC7_003563"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHVC7_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR2117516.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHVC7_003564"
     CDS             1086..1676
                     /locus_tag="ACHVC7_003564"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR2117517.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHVC7_003565"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHVC7_003565"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR2117518.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHVC7_003566"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHVC7_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR2117519.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHVC7_003567"
     CDS             complement(3788..3961)
                     /locus_tag="ACHVC7_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR2117520.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHVC7_003568"
     CDS             4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHVC7_003568"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR2117521.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4951..5178
                     /locus_tag="ACHVC7_003569"
     CDS             4951..5178
                     /locus_tag="ACHVC7_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR2117522.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHVC7_003570"
     CDS             complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHVC7_003570"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR2117523.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6751..7520)
                     /locus_tag="ACHVC7_003571"
                     /pseudo
     CDS             complement(6751..7520)
                     /locus_tag="ACHVC7_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1744 a         1891 c         1944 g         1941 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac cttgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatccggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
     4381 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt
     4441 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
     4501 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
     4561 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
     4621 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
     4681 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
     4741 ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
     4801 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
     4861 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
     4921 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
     4981 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
     5041 gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag cgatgaagtg
     5101 ttagtcaaag cctttgctaa ggccgataaa gattcgcttt tttggggcga acagaccatc
     5161 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt ttctgttatt
     5221 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
     5281 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
     5341 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
     5401 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
     5461 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
     5521 agtcgtgccc aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt
     5581 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
     5641 ggctgtcctg gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga
     5701 tttctcccca ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat
     5761 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg
     5821 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
     5881 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
     5941 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
     6001 tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat
     6061 cacacccggg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
     6121 tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct
     6181 gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg
     6241 tggtgagttg cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca
     6301 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
     6361 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta
     6421 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
     6481 atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct
     6541 tgttgaacgc ctgtttttct atatatatct tcaaaaacag gcaaggtttg acgcaaacta
     6601 tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa
     6661 tatcagcggc gttactaata cagaggcaat agtaccaata ataagagaag tgaggctaat
     6721 aacaatggtt tttagcacat gcctttgtca ttaaattgca taacgagtta aaaaatcaat
     6781 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
     6841 gtaaacgtca taatgattgc cagtgtttat taaataccca tactcaaggt cgtcatctgt
     6901 tgaaaaggat tcattcccta aggagattga tgccacattg ctgtaaacaa taaataatga
     6961 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcattaacct tctgctctgg
     7021 gtcggtgtct aaaacaaggc gtgccttgtt atcattaaat ataaattcgg gtaaatgcag
     7081 tatgcatttt ttgtcattca agcaactatc aataaattct tttttaaaag acaaaaaact
     7141 ccatgataaa aaaccaccct gcccctgaat tttgttgtca gattgtagat taacgccaaa
     7201 taacgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag cctcgatcgt
     7261 ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct tatggccgcc
     7321 cagcgcccat gaatactcat gttcttttaa aaccagtatc taaatcagat attcccggcg
     7381 tatttatctg ttctggctgg atactcatat tttcatcatc taaacaatac tcagaaaaaa
     7441 tatcatcaca cgcagtaatg ttttcaatat attccataaa gccataccaa taataacctt
     7501 taaaatctgt gatagactct
//
LOCUS       DAZPLM010000201         7492 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPLM010000201 DAZPLM010000000
VERSION     DAZPLM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.201"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            376..618
                     /gene="yiiF"
                     /locus_tag="ACHVC7_003572"
     CDS             376..618
                     /gene="yiiF"
                     /locus_tag="ACHVC7_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR2117524.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(935..1864)
                     /gene="fdhE"
                     /locus_tag="ACHVC7_003573"
     CDS             complement(935..1864)
                     /gene="fdhE"
                     /locus_tag="ACHVC7_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR2117525.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1861..2496)
                     /gene="fdoI"
                     /locus_tag="ACHVC7_003574"
     CDS             complement(1861..2496)
                     /gene="fdoI"
                     /locus_tag="ACHVC7_003574"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR2117526.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2493..3395)
                     /gene="fdoH"
                     /locus_tag="ACHVC7_003575"
     CDS             complement(2493..3395)
                     /gene="fdoH"
                     /locus_tag="ACHVC7_003575"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR2117527.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3408..6458)
                     /gene="fdnG"
                     /locus_tag="ACHVC7_003576"
     CDS             complement(3408..6458)
                     /gene="fdnG"
                     /locus_tag="ACHVC7_003576"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5871..5873),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR2117528.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6652..7485
                     /gene="fdhD"
                     /locus_tag="ACHVC7_003577"
     CDS             6652..7485
                     /gene="fdhD"
                     /locus_tag="ACHVC7_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR2117529.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1702 a         1979 c         1996 g         1815 t
ORIGIN      
        1 ttcccgaact gttaccaaca aaattataac gatgagcaag taaagaccgg ttggtggcag
       61 tactgaccgg tctgtgatga aagattaatt tcaccttaaa tcctggtgct gttaggatgc
      121 tgacgttttt cggctacacc attcagatta tgcaaataaa tatccctgaa tataatatat
      181 tcatagcacc cataactcat cctgggcgat aactaaacca tttttgagta ataaatgaaa
      241 caactgcaac gctcttatta tttctggaaa acgcctcgca aaaataaaaa atgatgcgta
      301 aatgagcttt ctttaaagat tcggcaaggg tacttttctc tccatagatt caaaaatgat
      361 attggcgaga tatttatgaa ctctctggca ggtatagata tgggcagaat tttactcgat
      421 ttatcgaatg aggtgattaa gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt
      481 gcggacctat taagggaagc ggtagatcaa tacctgataa atcaatcgca aacagcaaga
      541 accagtgttc ctggcatctg gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag
      601 ctgcgcgagg aatggtaatg ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg
      661 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaagtcat gcaaattcaa
      721 tatattgaag tttattggta ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt
      781 catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag gcaaacagac
      841 aattgcggca tgatcttgaa atacgagaga tggtgtcgtc acaccctatg agctgtaatc
      901 cgggcgacct gcgccacccg gaaaacgtca gagattactc tccttcaccc ggaaacagga
      961 acgggttaat ggaactacgg gcgtagcctt cttgctccat tcgcgcgtcc agtaccagag
     1021 aggcgaggtc atctgccacg gcttcaactt tcggttcttt ttcctgataa agaatcttca
     1081 ggtaagtgcc gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg
     1141 accagtaatg cagtttgccg ctctgctcgc agttgctgca ttttacgcgc actacgtgcc
     1201 attcggtttc acacaggttg cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca
     1261 ccatgctgga caccggcata gagccacata ccgggcaata ttgacgttgt tcgccgtatt
     1321 cagcgcgggc tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagcgcag
     1381 cccagataaa cggcgcttta tcgctgctga cggacgagaa atcagaggca aacagcgcgc
     1441 tggccatatc ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg
     1501 ccgggccgct catttcaggt ttcagctcag caattagcgc catcagcagc ttttgccagt
     1561 gcttatcacg cggcagaacg tgaatatcca gcgggggctt gccctgtgcg ctggcttctt
     1621 taatgcgcgc ggtcagatcc atctccagcg gatggtcgta cagcaccact tcctgggcgt
     1681 gggcgataag cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc
     1741 gcagacgctc ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa
     1801 tcatatccgc cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac
     1861 tcattcagct ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt
     1921 cttcgcccag gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca
     1981 aagggcggcg tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg
     2041 cagcattaac gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca
     2101 gataatcacg ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca
     2161 tttctgaccg aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg
     2221 aatattcttc gcccaaaaga tatcatcccg attgattagg ttgtgatgcc agtaacggaa
     2281 aaacatgatg atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc
     2341 cagctgcggt gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag
     2401 cccgctcacc gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg
     2461 cgcggtgtag cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg
     2521 tgcagattat tctcttcctc atccgcacgg ttcggaccga cacccacgta gtggaagata
     2581 ctggccgcaa aggtagccgc aaagccaaca gccgcgagcg gtttccagat gcctttccag
     2641 aatttcacgg tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg
     2701 tcagcatggt gcagcacgta catgacgtgt gtaccaccga cgcctgccgg atcgtacaga
     2761 cccgcattgt cgtaaccgcg ggttttcagc tcagcaacgc gctcgctcgc cagcgttttc
     2821 atcgactctt tcgtaccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt
     2881 tcttgcccaa ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc
     2941 gggttgaggc gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac
     3001 tgctcggact ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg
     3061 cacgctttca ggcagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat
     3121 tccagtttgt cgttctgctc cacttccgag aagcgcatca ccgtccacga tttggcgctt
     3181 aaatcattgg ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc
     3241 cactctgaac acgccacctg acaggcttta cagccgatac aggtggtgac gtcgatgagt
     3301 ttcgccactt cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg
     3361 gaacgacgaa tgatatcttg cgattgataa gccatatgtc gtctccgtta caccttttcc
     3421 acattcacaa ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc
     3481 gttaacgtat tggcaataaa gcctttttta gctacgcctt catagcccca gtgaatcgga
     3541 ataccgatgg tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc
     3601 accgctttgg ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca
     3661 atgccaagtt tattcgccag cgactccccg atttccacaa actgctctgg ttgcaaaatc
     3721 gcgttcaaca gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt
     3781 ccgacatacg ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagattcgc
     3841 gcagccgggt tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac
     3901 ggctcgtagt gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc
     3961 atgccttcct gctgcatgat aaacggcccg acgccgctgc ccggaggcgc tgcgctgtag
     4021 tccggaatat cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga
     4081 tcccacgggt taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc
     4141 ggccatgccc atgcccagcc cagcgtgtta ccgaggccag acggatcggc gttatcacga
     4201 cgcgccatct ggttgccttc cggcgtccag ctaccggcga aaatccagca gccacaggag
     4261 gttgtaccgt catcgcgcaa ttgggcgaac gagctaagtt gttggccttt cttgacgata
     4321 accgcaccgg ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgcc
     4381 acttcttccg acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctga
     4441 tccgggtttg cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag
     4501 aggatctcgc catcagtcag cgcaatccct ggggcgtccg cacctttcca gtgccactgc
     4561 aaccagcgac ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga
     4621 cggaacactt cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc
     4681 cagaagttag aggtttcagt gttcagcggg tcgatggtga cgaggaactt cagtttcgac
     4741 agacaaccga tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag
     4801 atatagccat tgaccttgcc ctctttcatc atctcgaagt attgcaggac gtcgtagcct
     4861 ttatcccact tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc tttatcacca
     4921 aagaaggcct tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttacctgg
     4981 ccttccagca gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc
     5041 tcgcttggca gcgtcatgta acctggcagg ctctgcgaca gcagccccag gtccgtcagc
     5101 ccctgaatat tggagtgacc gcgcagggcg ttaacgccgc cgcctgccat ccccatattg
     5161 ccgagcagca gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc
     5221 gtccagccga gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg
     5281 tattcgcaga ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct
     5341 ggcgtgtagc gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc
     5401 gtggtatcgc gtttggcgaa gccgttttcg tccagttcgt aagtccagga ggatttatcg
     5461 tacttgcgtt tttccgcgtc atagccggtg aacaggccat cttcaaagcc gtaatcctca
     5521 cgcacgatca ggctggcgtt ggtataggct tcggtgtatt cgcggttgaa tttttcattg
     5581 ttcagcaggt acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg
     5641 gcatagtagt cagccaccgc cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc
     5701 gcgccattgt gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg
     5761 gcgttaccgc ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc
     5821 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc
     5881 gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg
     5941 gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc
     6001 acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg
     6061 atgcgatcaa acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg
     6121 tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc
     6181 ggacaaagtg caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat
     6241 gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa
     6301 caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc
     6361 gcgagtgcta cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca
     6421 gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat
     6481 tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt
     6541 gcggaagggg ccgcaaaggt ccagaattgg ctcaatttcc ctccatccag gaagggtttg
     6601 taacagaata ccataatgtt ggtgtgtgtg ttcttatctg gttaagagaa agtgaaaaaa
     6661 acacagcgaa aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta
     6721 tggcggcgtg atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt
     6781 gcgctggtct acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag
     6841 tactttgcgc tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc
     6901 ggcatggatg tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc
     6961 cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta
     7021 tgcggcgtgg agcaacttaa tgacatcgga aaaccggtgc agccgctacc gttcacccag
     7081 acgtttgatc tcaacaaact ggatgatgca ttacgtcatc tcaacgattt tcagccagtg
     7141 gggcaactga ctggttgtac tcacgccgct gcctggatgt tgccatctgg cgaactggtc
     7201 ggcgggcatg aagacgtggg tcgccatgtg gcgctggaca aactgttagg ccgccggtca
     7261 caagaagggg aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa
     7321 atggtgcaaa agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc
     7381 acgcttgctg tagaagtggc tgagcgctgt aatctgacac tggtaggttt ttgtaaaccg
     7441 ggtcgggcaa cggtttatac ccatccgcag cgtttgagca attaattaaa at
//
LOCUS       DAZPLM010000202         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPLM010000202 DAZPLM010000000
VERSION     DAZPLM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.202"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHVC7_003578"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHVC7_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR2117530.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHVC7_003579"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHVC7_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2117531.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHVC7_003580"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHVC7_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR2117532.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6941..>7424
                     /locus_tag="ACHVC7_003581"
     CDS             6941..>7424
                     /locus_tag="ACHVC7_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR2117533.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1775 a         2057 c         1942 g         1650 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccaag
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt
     6781 tatcagatat tttagataac gtaatattaa catattaata acattaactt tttaaaaact
     6841 ggcatccgct ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc
     6901 ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt
     6961 acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt
     7021 tgaactgaat aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc
     7081 gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc
     7141 gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc
     7201 tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat
     7261 ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca
     7321 gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata
     7381 tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gatt
//
LOCUS       DAZPLM010000203         7365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPLM010000203 DAZPLM010000000
VERSION     DAZPLM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.203"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            188..1252
                     /gene="ulaG"
                     /locus_tag="ACHVC7_003582"
     CDS             188..1252
                     /gene="ulaG"
                     /locus_tag="ACHVC7_003582"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR2117534.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1360..2115
                     /gene="ulaR"
                     /locus_tag="ACHVC7_003583"
     CDS             1360..2115
                     /gene="ulaR"
                     /locus_tag="ACHVC7_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR2117535.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2112..2861)
                     /gene="yjfP"
                     /locus_tag="ACHVC7_003584"
     CDS             complement(2112..2861)
                     /gene="yjfP"
                     /locus_tag="ACHVC7_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2117536.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3043..3372
                     /gene="bsmA"
                     /locus_tag="ACHVC7_003585"
     CDS             3043..3372
                     /gene="bsmA"
                     /locus_tag="ACHVC7_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR2117537.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3521..3796
                     /gene="yjfN"
                     /locus_tag="ACHVC7_003586"
     CDS             3521..3796
                     /gene="yjfN"
                     /locus_tag="ACHVC7_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR2117538.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3913..5538)
                     /locus_tag="ACHVC7_003587"
     CDS             complement(3913..5538)
                     /locus_tag="ACHVC7_003587"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR2117539.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5622..6785)
                     /locus_tag="ACHVC7_003588"
     CDS             complement(5622..6785)
                     /locus_tag="ACHVC7_003588"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR2117540.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6788..>7365)
                     /locus_tag="ACHVC7_003589"
     CDS             complement(6788..>7365)
                     /locus_tag="ACHVC7_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR2117541.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1926 a         1963 c         1798 g         1678 t
ORIGIN      
        1 tttatttaaa ttcatttagt taatttaact acacattcca ttaaatcttt ccacactcaa
       61 aatcaacaac aacttccctg gcaaaaagtc aaaaaatggt cattgccttc ctgaaaaaat
      121 agtgtcagga ttaatcaaaa ccaatcacat attgattcga attgaactat catgaaggta
      181 aatggcgatg agtaaagtga aaagtatcac ccgagaatcc tggatcctga gcactttccc
      241 ggagtggggt agctggctga atgaagaaat tgaacaagag caggtcgccc ctggcacatt
      301 tgcgatgtgg tggctaggct gcaccgggat ctggttgaaa tcagaaggtg gcaccaacgt
      361 ttgcgttgat ttctggtgcg gcactggcaa acaaagtcac ggtaacccgt taatgaaaca
      421 gggtcaccag atgcagcgca tggctggcgt gaaaaaactg cagccaaacc tacgcaccac
      481 cccgtttgtt cttgatccgt ttgcgattcg ccagatcgac gcggtactgg cgactcacga
      541 tcacaacgat catatcgacg ttaacgtcgc tgctgccgtg atgcagaact gtgctgatga
      601 cgtaccattc attggaccga aaacctgcgt tgatttgtgg attggctggg gcgtaccgaa
      661 agagcgttgc atcgtggtca aaccgggcga tgttgtaaaa gtgaaagaca ttgaaattca
      721 tgcgcttgac gctttcgacc gtactgcact gatcaccctg cctgccgatc aaaaagcggc
      781 tggcgtactg ccagatggca tggacgatcg cgcagtaaac tacctgttca aaacccctgg
      841 cggttcccta tatcacagtg gcgattccca ctactctaac tattatgcga agcacggtaa
      901 cgaacatcag atcgatgtgg cgttaggatc gtatggtgag aacccgcgcg gtattaccga
      961 caaaatgacc agcgccgata tgctgcgtat gggtgaagcc ctgaatgcga aagtggttat
     1021 cccgttccac cacgacatct ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct
     1081 gtgggagatg aaaaaagatc gtctgaagta tggcttcaag ccgtttatct ggcaggttgg
     1141 cggcaaattt acctggccgc tggataaaga caacttcgag taccactatc cgcgcggttt
     1201 cgatgattgc ttcactattg aaccggatct gccgttcaag tcattcctgt aatctgtcgt
     1261 ttataccgga tagcgtggag aagttcgcgc tatccggcgt agtatttcct agctatttca
     1321 aatatcatct aaaaaaatca aattttatcg gaatagctca tgactgaagc acaaagacat
     1381 caaatcctcc tggaaatgct cgcacaattg ggctttgtga ccgttgagaa agtcgttgag
     1441 cgtctgggaa tttcgcctgc cactgcgcga cgcgatatca ataaacttga cgaacgcggc
     1501 aaactgaaaa aggtgcgcaa tggcgcagaa gctattaccc aacagcgccc gcgctggacg
     1561 ccgatgaatc tgcatcaggc gcagaatcac gatgaaaaag tacgtattgc taaagcggcc
     1621 tcgcagctgg ttaatccggg cgaaagcgta gtcatcaact gcggctccac cgcgtttctg
     1681 cttgggcggg aaatgtgtgg caagccagtg caaatcatca ctaattatct accgctggca
     1741 aattacctga tcgatcaaga acatgacagc gtgatcatta tgggtggaca gtacaacaaa
     1801 agtcagtcca tcactttaag cccgcagggc agcgaaaaca gtctctatgc cgggcactgg
     1861 atgtttacca gcggaaaagg gctgaccgca gaagggttgt ataaaaccga tatgctgaca
     1921 gcaatggcag agcagaagat gctgagcgtg gtagggaaac tggtggtact ggttgatagc
     1981 agtaagattg gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat cgatatgctt
     2041 atcaccggca aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca aggggtcagc
     2101 attctgcgtg tttaaagatg ctggcggaaa aatgtcacgg cagcatctaa cgcctcaggc
     2161 gtaatgcggt gacgcacgcc tggctgccat gaacaggtta gcagtttatc ccgtcccgtc
     2221 tcgcttaagg cctgctgcaa acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg
     2281 ccatgccaca gcagtagagg tctgtcccca agttgttcca ggtggtttgt cgcttcccac
     2341 tctgccagcg gcgcgataat gttattgaat tcattctgct gtgctgccgt ttcaggtatc
     2401 agcggtggaa acagtgaacg ggcgagcgat gtaaaatagc ccgatcccat catgctggcg
     2461 gtacatttca ccgtggggtg gcgagcggta attcccagtg ccgtcatcgc gcccatcgac
     2521 gcgccaccga ctgccagacg gtcatcaagc agccagtttt cttcggctat tgccgcacgt
     2581 aaagtagtga attcctgcat actttgtagc aagatttgcc agaattgatt taaccgccgc
     2641 gcttcgtcgc cactaaaacg gctaccgtga tcgggcgcat ccggcatgat cacccgcaaa
     2701 ccagcctgcg ccagcgcaac ggcaaaatag ctatacacca ggctggatga agtaaagccg
     2761 tgataaaaaa ttacgcacgg taacggggta tctttttgcc cgacaggata agcatgaaga
     2821 acgggaatat ctgccagctc gcgtgattct atttcaatca tctgtctctc ctttttgtct
     2881 ggtatgacat gaagaaatcc ttagcacaag tttctgctgg cggtattagt tcataatgtt
     2941 gagatgtggg ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt
     3001 cgggaacatt cccccaaagt taaattaata aggcactaca ctatggttag caggaaacgt
     3061 aatagcgtta tttaccggtt tgccagttta ttattggtgt tgatgttaag tgcctgtagc
     3121 gcactgcaag gtacgccaca gccagcaccg ccagtaacgg accatccgca agagattcgc
     3181 cgcgaccaga cgcaagggtt acagcgaata ggtagcgtaa gcacgatggt tcggggttct
     3241 ccggatgacg cattagcaga aatcagagca aaagctgtcg ctgcaaaagc tgattattac
     3301 gttgtcgtca tggtggacga aaccatcgtg acaggacagt ggtattcaca agccattttg
     3361 taccgtaaat aaatcagaca acttacatag tcagctttac attgctttgc gtccctatgc
     3421 gtttacctgt gcacaataaa ttaccggctg gaagcctggg cgtttattac cgcgatggaa
     3481 tgccctgcaa catgtgggga aacgaaaaat ggagctgacg atgaaacaat tacttgcctc
     3541 accctcgctg caattagtga cttatcctgc gagcgccacg gcacagtctg cagaattcgc
     3601 cagtgctgac tgcgtgacgg gcctgaatga aataggtcag atctcagtta gcaatatttc
     3661 aggggatccg caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc
     3721 atggtaccgc attattacca tgtacgaaga ccagcagcct gataactggc gcgtacaggc
     3781 gatactgtat gcatgagtgt catctacagc aaatgaacaa tgccggatgc ggcgcgagcg
     3841 ccttgtccgg cctacaattt tcacatattt cattgagtta atcgaaacca gcgtcgcatc
     3901 agtcgtatac gtttacaaac acactccccc cgtcgcccgc agcaataaat cattctggat
     3961 ctgctctgac aaccgtacgc cgccgcgcgt atctaacatc acctgacacc acgcctgcgc
     4021 cattggcgga gaagcatatt tcaacatttg cgcaccgcag cccagcagga atagctgatg
     4081 agtaatctct cgccccagtt cttcagctgg cttacgcagc tgctgctgta aacgacgaac
     4141 cgcgcgatca aaatagcgat cctgcccttt cacttccaca aatgcttccg acaataagtc
     4201 gtatacgccc gcttgcttat tgagaacgcg caacacatcc aggcacataa tattgccgga
     4261 accttcccaa atactgttta ccggcatctc ccggtaaagc cgcggcagct cgctctcctc
     4321 gcaataacca atgccgccca gcacctccat cgcttcggcc acaaacggca tacctcgttt
     4381 gcagatcaca aattttgccg caggcgtaaa taaacgcgcc cacagggctt ctttggcatc
     4441 ggcgcgccgg tcccacgctc gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg
     4501 aagtgccatg cgacttaaga catgacgcat aaggggctgt tggatcaatg gattaccaaa
     4561 aacatggcgt tgatgtgcat gataaatcgc cagcgaaaac gcacggcgca tcatggcatg
     4621 gctacccagg gcgcaatcaa aacgcgtcat cccccccatt ttcaggatca gacgaattcc
     4681 ttccccttcc tgccccaata accagccaat ggcatcctga aactccactt cacaactggc
     4741 gttagagcga ttacccagct tatctttcag ccgttcgagg cgaatcgcgt tgcgttgccc
     4801 gtcaggcaaa aagcgcggca caaaaaaaca ggacagaccg cccgtggtct gcgccagcac
     4861 cagatgcgca tcgctttgcg gaaccgagaa aaaccattta tgccccacca gccgataaga
     4921 gccatcttcc aaacgctctg cacgggtggt gttgctcatg acatcggaac caccctgctt
     4981 ttccgtcatt cccatgccaa tcaacaaacc gcgtttttgc ccacctggca ataagtgaga
     5041 atcgtagcga tcgctcagca gcggcgtggt ccagtcctga aacggcgtgg gtaacatctg
     5101 taacaacaat ggcgtggcgg caaaggtcat cgttattgga cataacgacc cagcctcaac
     5161 ctgcgcatgt aacataaaac gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc
     5221 ttcccaggca agattgtgca cccgattggt acataacacc tgcatcagca ggtgccatgc
     5281 gggatggaaa cgaacatcgt ccagacgccg cccctgggca tcgtagcgca acagttcagg
     5341 cgggtttaca ttcgccagcc gccccagttc aagggattca gccgttccta actgctgacc
     5401 aatactggct agaaaatcgc tatcccagcc agcaccttca cgcgttaccg cttcgcaaag
     5461 cgcgccatca gacaggtata agttgctgtt atttaatggt ataggttgat taaaaacggt
     5521 gtgagtttgc cagtgcactg tgtctccctc catcaatggc agtcaccatt agtatggcca
     5581 ctgccatgga ttcatgacag aaagagattg ctaaaacatt cttatccagc aatgtaatgc
     5641 ggaacgaaac gtgaggtgtc tttggtgatc agtgtgttat cttcacggat ccccattccg
     5701 cacgcttcat catcgacaat ccagctatcg atgagtgtgt agctatcgcc aaatcgcggc
     5761 agaggttgaa acgcctggta gatcatcggt tcatcggcgt aatcaccatc agcgtggtca
     5821 acgacgttat tctgaccgtc gaaaatggtg acgtttccgc cttcgcgcga gtagatcggt
     5881 ttacgcacat agctttcgcc agcggcaatc tgcgattttt caccctcaaa ccaggatgca
     5941 agaaggttag gatgaccagg gaagaagcgc caaagcagcg gcattagccc tttattactc
     6001 aagatacttt tccataacgg ctccacccat tgctcgcgac gcttgcacag cagcggaccg
     6061 ttatcgtcac gcatcatcca ctccagcgga tacagcttaa atgcacgctg gatgacatta
     6121 tcatcaagat cggtcagtac accgccgacg cccaaaccga gatcttcaat gtagataaac
     6181 cgcgactcct gccctgcctg ctgggcgcag tcctgcaagt acagcacggt agtcctgtct
     6241 tcatcggtgt cctgacagca gcagaaataa aacggctccc ggctataaag ctcgctaaag
     6301 cgtgaaatca ggcgttcctg aatggcattg tactgatcgg catcacgcgg aataatgccg
     6361 ctgcgccggg catcctccag ccacaaccac tggaaataag ccgactcata caacgaagtc
     6421 ggcgtatcgg cgttgtactc cagcagcttc accggcgcat tgccacacca ggcaaaatcc
     6481 atccggccat acagcgaagg atcgctggcg tgccagcttt cggcgatcac atcccagtac
     6541 aacggcggga tcgccagttg cgtcaggatc tcttcgtctt taaccgcgcg atccaccacc
     6601 tcaaggcaca tctgatgcag ttccgcagtt ggtttttcga tctgctcttc aatctggcgc
     6661 agagtaaaac ggtaagcccg actctcgtcc caatagattt cattgtcgat gatatgaaag
     6721 tcgaaaccgt tgtcagcggc aatttggtcc agatcccgcc gcacaggaac gttgtgtctc
     6781 agcatgatta gcctccccaa tgcccacggg cgctggaaga acgaccatag ccgccgcgcg
     6841 aaacggttga cgcttttttg gtggtaaagc cgcctgaaga gtaagactct tttttgccag
     6901 aaccggagcg ccaggagtaa tcaccggaag tgttgcgcca gacggggcgg gaagcaaatg
     6961 aggatccgcc gctgttatag acgaacggct catcgcgatc tttacgaata acccggctta
     7021 acaaaaatcc agaaacgacc gggatccaac tctgttcaac attgtcgtag tagcaatttt
     7081 cgtacttaga ctgacagttc tgctgagtca tattcttcgg aacatcggca taaaatgccg
     7141 ttttggcgtt gttccagcca cgcgcgcaaa tgtcggcatt attaccgtca tcaatacaat
     7201 cctgcactgt cgcgtaaaac gttccgtcgc cgtcgttatc gacatcgcta ctatcgccac
     7261 aacctttcaa tacgaaaaaa gcggcaccgc ccattagcgc caacgttaag tatttttgtg
     7321 cgtagcgatt gcgacgcggt tcaaaaggat tattcggtct accaa
//
LOCUS       DAZPLM010000204         7354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPLM010000204 DAZPLM010000000
VERSION     DAZPLM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.204"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            86..421
                     /locus_tag="ACHVC7_003590"
                     /pseudo
     CDS             86..421
                     /locus_tag="ACHVC7_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            418..873
                     /locus_tag="ACHVC7_003591"
     CDS             418..873
                     /locus_tag="ACHVC7_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117542.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHVC7_003592"
     CDS             complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHVC7_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR2117543.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1987..2895
                     /gene="mak"
                     /locus_tag="ACHVC7_003593"
     CDS             1987..2895
                     /gene="mak"
                     /locus_tag="ACHVC7_003593"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR2117544.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHVC7_003594"
     CDS             complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHVC7_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR2117545.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4348..>7354)
                     /gene="sbcC"
                     /locus_tag="ACHVC7_003595"
     CDS             complement(4348..>7354)
                     /gene="sbcC"
                     /locus_tag="ACHVC7_003595"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR2117546.1"
                     /translation="LDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGE
                     AYRAFWSQNRARNQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFT
                     RSMLLSQGQFAAFLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQ
                     ASGVTLLTPEQVQSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQ
                     ALQQALAEEEKAQPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQS
                     TMALRASIRHHAAKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDRE
                     HLRQWQQQLTHAEQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQ
                     QKRLAQLMVTIQNVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQ
                     RAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLD
                     ALTKQLQRDENEAQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQL
                     RLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATR
                     QQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLA
                     LHSQQQTLQQQDVLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLK
                     QNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTS
                     QGEIRQQLKQDADNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLT
                     LDNLVHLANQQLTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSL
                     ALALALSDLVSHKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEA
                     MKERIPVQIKVKKINGLGYSKLESTFAVK"
BASE COUNT         1698 a         1907 c         1906 g         1843 t
ORIGIN      
        1 tcctttgctg cgattttttc aatttccgat atattcataa ttcatcaagg ttgatataaa
       61 tatcagtgaa gatctccagg tattttttgc ggaactggct acgataaaag ataaatcaga
      121 tgatgaatgg tggcgtgcat tgctgcaaag tcgcaatgat catttgcgcc aaacggcatt
      181 acgcaacgca cataccccgg cgtcgttgtt aacgacattg actgagtctc aggatcggtc
      241 gctggctatt aataatccac agctggctgc cgatgtgaaa acggcgtggt taaaagagga
      301 tctatcatta ctcttatttg tcgaacaacc cgatctttcg cagttacgtg atttagtgaa
      361 aaccggggca acgcggaaaa ttcgcagtga agcgcgtcac cggcttgagg aaaaacaatg
      421 acacaacgtc catggagcaa gttacagcgg gagatctatg acttactgac gccaacgatc
      481 aacctgcaaa ttcactgtac ccgttatctc atgcgcagcc aaaatggtgg aagtaccgat
      541 ttaccgcgtt attggatcac gttggataaa aacgtgatct gggattaccc gaaagacttt
      601 attgcaggta acgctggagt gcgtaatttt cacggtgaaa cttgctggta tccttacctg
      661 acggatattt gttcaatttc tgatttattg cgtgaatata ttgatacccc aaaagcggag
      721 ctgttaacaa aacaatttac cagcgataaa tgggggttag tgaatattct gcgtgctgct
      781 gacaggcgaa tagggatgag gcgattagat cagttacggc gtaaaacgca taatattgct
      841 gcgctaaaaa ttattgctcg ccgtagcgaa taattatatg cctggtgtgg cttcgtacgc
      901 cggataagac gcggcaggcg tcgcatccgg cattaaagga aaatcagcaa ttaacgttgt
      961 gcttcgccac ctaatccttc aatcaggttt tgaattaacg ctgccagttc accagtcatc
     1021 aggatgaaat cggcatcaaa acgcagggcg aaatcttcac ggtcgatatc ttcgttttga
     1081 tcgcgcagct cgtcgcagaa cttaagacgc ttgagcgaac catcgtcgca catcacaaac
     1141 tgaatgcgct gctgccagtc gagcgccagt ttagtcacca cttttccggc ttcaatgtga
     1201 ttggtgatct cttcgctggt cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc
     1261 agcaacgatt tcagctcggc ttcatcaagc agctggaagc cctgtgccgc actaccggag
     1321 cgaacccatt cggtcagcgt cagttcaatc gggttttcca tgctcaatgg tacaaccggt
     1381 aacgacccca ggcttttacg cagtaatgcc agcgtatctt ccgctttttt ggcactggcg
     1441 cagtccacca taatcaaacc gttaaccgtg tcgatccaca tcattgtctg gctaaaacgg
     1501 ctgaaagcac gcggcagcag agagtgcagc acttcgtctt tcagcgaatc tttttcggtt
     1561 ttcttcagct tacgcgcctg ttccgcttcc agtttggcga ttttcgcttc cagcgcctgt
     1621 ttaatcaccg gagacgggag gattttttct tctttgcgcg cgcagataac aatttgacca
     1681 ttggcaacgt gcgttaacgc atcgctgtgc gatcccatcg gaggaaccca gcccatcttc
     1741 gccatgtcct ggctgccgca tggggtaaat gccatcgagg ctagctgttt ttccatctct
     1801 tctgcacgca gcgaaatctc gcggctaaga cggtaaacca ttaaattttt gaaccacagc
     1861 atgataattt ccacggcctt gtcgttaaat ttagcgggca tgataacgaa ttgtcggcgg
     1921 ccttgcattg ccaatccggt tgtccgtctc tacgctattg atattgaaaa aaataaggag
     1981 agtacagtgc gtataggtat cgatttaggc ggcaccaaaa ctgaagtgat tgcactgggc
     2041 gatgcagggg agcagttgta ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag
     2101 actattgaaa cgatcgccac gttggttgat atggcggagc aggcgacagg gcagcgcgga
     2161 acggtaggta tgggcattcc aggttcgatt tcgccctaca ccggtgtggt gaagaatgcc
     2221 aattcaacct ggctcaacgg tcagccattc gataaagact taagcgcgag gttgcagcgg
     2281 gaagtgcggc tggcaaatga tgccaactgt ctggcggttt cagaagcagt agatggcgcg
     2341 gcagcgggag cgcagacggt atttgccgtg attatcggca cgggatgcgg cgcgggcgtg
     2401 gcattaaatg ggcgggcgca tatcggcggc aatggcacgg caggcgagtg gggacacaat
     2461 ccgctaccgt ggatggatga agacgagctg cgttatcgcg aggaagtccc ttgttattgc
     2521 ggtaaacaag gttgtattga aacctttatt tcgggcacgg gattcgcgac ggattatcgt
     2581 cgtttgagcg gacatgcgct gaaaggcagt gaaattatcc gcctggttga agaaagcgat
     2641 ccggtagcgg aactggcatt gcgtcgctac gagctgcggc tggcaaaatc gctggcacat
     2701 gtcgtgaata ttctcgatcc ggatgtgatt gtcctggggg gcgggatgag caatgtagac
     2761 cgtttatatc aaacggttgg gcagttgatt aaacaatttg tcttcggcgg cgaatgtgaa
     2821 acgccggtgc gtaaggcgaa gcacggtgat tccagcggcg tacgcggcgc tgcgtggtta
     2881 tggccacaag agtaaaaaac gtaggcaatt ggcgcatcat gcctgatgcg acgcttgccg
     2941 cgtcttatca ggcctacaaa atgtgccaga accgcgtagg gcggataagg cgttcacgcc
     3001 gcatccggca ataagcaacg cgcaccgtga cagtaatcta ccccagtggt ttcgccagca
     3061 ccggactatc cgccgcttgc tggcgcttat agcgaccata cagcagcaac gatgacatcg
     3121 cagcaaacga aagcagggcg gcaggcagcg ccacgtaatt atatgccagc cccagcgtca
     3181 gcatcatacc tccgcaatat gcgccgacgg cgctaccgag gttaaacgct atttgcccac
     3241 ctgcggcacc taataactct ccgcctttgg cgttttgcag taacaatatt tgcagaggtg
     3301 ctgaaagggc aaataatccc gcgcaacaaa taaaagcaaa aataagcgac gttgttttca
     3361 tgccgccgca gaaaaagagc atcagcagtg ccagtacaat gataaagtca gtcactgctg
     3421 caatgcgcag tggtgaataa cgtcctgaaa tcctgccact tagcatattt cccagcacca
     3481 tccctagccc aactaacatc ataataaagg tcatcgccgt ttccgaaaaa acggaaataa
     3541 acatcatgta tggctttacg tagctgaacc aggcaaagac acctgcgttg ccaaacatcg
     3601 tggcggcgaa aattaaccac ggggccgggc tgcgcaaaaa gtgaaattgt tcgcgcagat
     3661 ttcctttcgc ctcgtcgcga atatctggca cccaaaaata gaccgatgcc atcaccgcaa
     3721 tattaaaaac agcgatcaat aaaaaggtgt aacgccagct aaattcctga cttaaatacg
     3781 ttcccagcgg aatgcccagc aaattggcga ctgtcatccc ggaaaccatc cccgccacgg
     3841 cggcggtgac ttttccgggt ttgataattt ttgataacac gatcgcgccg acgccaaaaa
     3901 atgcgccatg cggaaagccg gaaaccaggc gaccaatggc aagcatcagg taagacgaag
     3961 agagcgtgaa catggcgttg ccaatgacgc acaacgccac cagaaacaac aagatatgtt
     4021 tgagtgagta gcggctggaa aagagtgcga tgattggcgc accgaccacc acccccagtg
     4081 cataatacga gatcatatgc ccggcagcag gaatcgaaat tcctacgtta tgcgccagct
     4141 ccgtgagcac gcccataatg ccaaattcgg ccatccccaa accaaacgtg cccagagcca
     4201 aagataaaat gacttttttc ataccaccat cacaaccacc agaacggcta ccaatcaccg
     4261 tagccaataa agtgatagct tgcaggataa cgaccagcaa ggccaagaga taattcgccc
     4321 ctctgtattc attatcctgc tgaatagtta tttcactgca aacgtacttt ccagtttgct
     4381 gtagcccagg ccgttgatct tcttcacttt gatctgcaca ggaatacgct ctttcatcgc
     4441 ttcaacgtgg ctaatcacac cgatggtttt gccactggcg ttcagggcat ccagcgcatc
     4501 aagggcggta tccagcgttt cgctatccag cgtgccaaaa ccttcatcaa ggaacagcga
     4561 gtcaatacgt gttttatggc tgaccagatc cgaaagcgcc agcgccagcg ccagactaac
     4621 gaggaaactt tcgccgccgg aaagggtacg ggtatcgcgt actgcatctg cctgccaggt
     4681 atcaacaacc tcgacttcca gcgcctcgct ggctttgcgc tgtaacagat agcgcccgtg
     4741 cagccgggta agttgctgat tagcgagatg gactaaatta tccagcgtca gcccctgggc
     4801 aaacttgcgg aatttatcgc cctctttgga acctattagc gaattcagat atccccagtc
     4861 ctcaacctgc tgcgtcattt gagcaatttg ctgcagtaag gtttgttgtt gctgacggtt
     4921 atctgcatcc tgcttcagct gctggcgaat ctcgccttga ctcgtggtgt tttcacgcaa
     4981 cttttggtga gtttgcgcta actcttgctg aatctgctcc accgtcacag tgagagccaa
     5041 cccgtcaggt cggtgttgtt gatgctgtgc cagcgtttct gctgtctgtg tgaccagcgt
     5101 ttgcgcctga cggcgctggt tttccagatt ctgcttgagc tgttccagct gcgttagtgt
     5161 ttgttcatcc attagcgccg caaggaacgc ctgctgatcg tcaaagacgc tggcctgtag
     5221 cgcggtgtca aactgcgcct gggctttttg cagactttgc gccgccagaa catcctgttg
     5281 ctgtaacgtc tgctgctggc tgtgtaatgc gagacattgt tcatgaacct gccgccagtt
     5341 atccagcgcc acagtttctt cgctgtgcgg gagatcatca ctttgcggca acgtttccag
     5401 aatcggcgtc agctgctgaa tacggttttg cagcgcggtt aattcgttct ggcgttgctg
     5461 ccagctctgc gcttcttgct gacgtgtcgc caaccagctc tcttcttcat cttcctgtgg
     5521 caatgtcagt gcataacccg tcaatgccgt taagagctgc tgctggcgtt gttcaatttg
     5581 ctgttgatac tggataattt gctgattatg cgcggcaatc tgcccttgta attcatgtcg
     5641 ttggctgagt aaccgcagct ggcgttcgtg ctcatcttgt gcatccagcc acggttgaat
     5701 atcgtcctgt ggctgcaagg tgatattgag gctggtcgtg acggcttgcc attgttgagt
     5761 aagtgcttgc tcatcttgtc ggaggctttg cgcttcgttt tcatcgcgct gaagctgctt
     5821 cgttaatgca tccagttgcc cgcgcagcgc cgcaccttct tcgccgagct ttttaacttc
     5881 gttttccagc gtcagtaatc gagactgatt aacaccaggc tccagcgcct gatacgcctc
     5941 gaccgccggg tggctggtgg aaccacaaag tggacaaggt tgacccgcct gtaactgtgc
     6001 acgttgagct tccagcgttt tgatgcgcgc ttcctgctcg caaatggttt tcacatcggc
     6061 aagttgctgc gtcttttctt tataacgctg gcgcatttcg ttaagtgcga cattacgttg
     6121 cgtctgttcc agagtgacat tctggatagt gaccattaac tgcgccagac gtttttgttg
     6181 gggaacaatc tgcccatgca gcgcgaccag gcgctgacgc agtgggcgtt gctcggaatg
     6241 ttgcgccagg gcgctagcaa cttcatcggc ggttaacgtc aacgtgatcg ccgcaagcgc
     6301 attaagtttt tgctcagcat gggttaactg ttgctgccat tgccgcagat gctcgcgatc
     6361 gctggtttgt tgggagaact gcgcacgcca acccgccagt tcgttgttcc actgacggaa
     6421 gcggtcgtgt tcctgtaacc aggtattcag gctttgttgc tgctgctgta attctgctga
     6481 ctgcttcgcc gcgtggtggc gaatgctcgc gcgaagcgcc attgtgctct gtaagcgagt
     6541 atttacttct tcaatctgct ggcgaatatg cgccagcgcc gcgctgtgtt ctgcgatgcg
     6601 ttcccagtgt ggacgaagat ttcgtgccgg ttgtgccaga ctaagcgccg ccagttgagg
     6661 ttgcgctttt tcttcttcgg ctaacgcctg ttgcaaggcc tgctgacggc ggctggcttc
     6721 ttgctgcaat tcgtcctgac gcgttaacca gtttagcgat tgttgttctt gctgctgcgc
     6781 ggtaattaac tgtttttctt cgtcagtaag tacctgcaaa ctcgctgtca gcgattgcac
     6841 ttgttccggc gtgagcaacg tgacgccgct ggcctgcgct tgcagcttct ccagctctgt
     6901 gcgggccgat ttgtgctgct caaaaaccat cgccgagatt tgcccgtaga tttcagtgcc
     6961 ggttaactcc tcgagcaatt ccgcgcgttc tttgggtttg gcattcagga aggcagcaaa
     7021 ttgcccctgc gaaagcagca tcgaacgagt gaagcgcccg taatccagcc cggttaacgt
     7081 cgctgtcagt tccagcttat ctttcacttt gtcggcgaga attttgccgt cggcgcagcg
     7141 cgccagctct acgcgtggca cctgcaaatt accgtcgggt tggttacgcg cccgattctg
     7201 gctccagaat gcacggtacg cttcaccttt cacttcaaac tccacctccg ccagacattc
     7261 ggcggtatcg cgggtcatga gatcattttg cgattgtgaa acgttagaga gacgcggagt
     7321 ttcgtgatac agcgccagac aaatggcgtc cagc
//
LOCUS       DAZPLM010000205         7319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPLM010000205 DAZPLM010000000
VERSION     DAZPLM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.205"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            164..1465
                     /locus_tag="ACHVC7_003596"
     CDS             164..1465
                     /locus_tag="ACHVC7_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2117547.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            1732..3387
                     /gene="opgD"
                     /locus_tag="ACHVC7_003597"
                     /gene_synonym="mdoD"
     CDS             1732..3387
                     /gene="opgD"
                     /locus_tag="ACHVC7_003597"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR2117548.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3527..3751
                     /locus_tag="ACHVC7_003598"
     CDS             3527..3751
                     /locus_tag="ACHVC7_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR2117549.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3814..4350
                     /gene="rimL"
                     /locus_tag="ACHVC7_003599"
     CDS             3814..4350
                     /gene="rimL"
                     /locus_tag="ACHVC7_003599"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR2117550.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4345..5325)
                     /gene="ydcK"
                     /locus_tag="ACHVC7_003600"
     CDS             complement(4345..5325)
                     /gene="ydcK"
                     /locus_tag="ACHVC7_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR2117551.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5449..6441
                     /gene="tehA"
                     /locus_tag="ACHVC7_003601"
     CDS             5449..6441
                     /gene="tehA"
                     /locus_tag="ACHVC7_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR2117552.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6438..7031
                     /gene="tehB"
                     /locus_tag="ACHVC7_003602"
     CDS             6438..7031
                     /gene="tehB"
                     /locus_tag="ACHVC7_003602"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR2117553.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         1852 a         1713 c         1826 g         1928 t
ORIGIN      
        1 gataacttga ttgcaaaata ttattaacaa ttaaagcaat tatgttacag caaaatggat
       61 aatattgatg ttttcgcggc gagatcacag tttgtaaatt cttcccgcca gagtgaatgc
      121 ggttacctac actccagatt actgaccact ggaggcagac actatggcga acagcatcac
      181 ggcggatgag attcgggaac agttttcgca ggcaatgtca gccatgtacc agcaagaagt
      241 tccgcaatat ggcacgctgc tggaactggt agctgatgtg aatctggctg tgctggaaaa
      301 caatcctcaa ctgcacgaaa aaatggtaaa tgcagacgag ctggcgcgac tgaatgttga
      361 acgtcatggg gcgattcgcg ttgggactgc acaagagctt gctactcttc ggcggatgtt
      421 tgccattatg gggatgtacc cggtgagcta ttacgatctc tcgcaggcag gggtgccggt
      481 acattcgaca gcatttcggc ccattgatga tgcttctctg gcgcgtaatc ccttccgcgt
      541 ttttacctcc ttactccgcc ttgagcttat cgagaacgaa attttgcgcc agaaagcggc
      601 ggagattctg cgtcagcgcg atatcttcac cccacgttgt cgacaactgt tagaggaata
      661 tgagcagcag ggcggtttta acgaaacaca ggcacaggag tttgtgcagg aagccctgga
      721 aacgtttcgc tggcaccagt cagcaacggt agatgaagaa acctatcgcg ccttgcacaa
      781 cgaacatcgg ttgattgctg atgtggtctg ttttcctgga tgccatatca accacctgac
      841 gccacgtacg ctggatattg accgggtgca gtcgatgatg cctgaatgcg gaattgaacc
      901 caaaattctc atcgaggggc cgccgcgccg cgaggtaccg attttactac gccagaccag
      961 ctttaaagca ctggaagaga cggtgttgtt tgcggggcag aaacagggca cgcataccgc
     1021 gcgctttggt gaaattgagc agcgtggcgt ggcattaacg ccgaaagggc gacaactgta
     1081 tgatgatctt ctgcgtaacg ctggaaccgg gcaggataat ctcactcacc aaatgcattt
     1141 acaggaaacc ttccgcactt ttcctgacag tgagttttta atgcgtcagc aagggttggc
     1201 atggttccgg taccgtctga cgccttcggg tgaggcgcat cgtcaggcga ttcatcctgg
     1261 agacgatcca cagcccttaa ttgaacgtgg ttgggtagtg gcgcaaccca tcacctatga
     1321 agatttcttg cccgttagcg cggcggggat cttccagtca aatctgggta atgaaacgca
     1381 gacacgcagt cacggtaatg ccagtcgcga agcatttgag caggcgttgg gttgtccggt
     1441 tttggatgag ttccagcttt actaggaagc ggaagaacgc agtaaacgtc gctgtggttt
     1501 gctttaaatt ctgaccatcc gcctttgcaa aaatttgcct gattttacaa acgaatcagg
     1561 ctcatcccat cgacataaaa aaaatgccga tttatgcata ttctctcagt tcaacaattg
     1621 gattattaat aaatattgtc tagagtgagc ggtcataaat aagcactttc ttgccgctga
     1681 aaacgaccag cgcgggacca ttcacaacac cagaaggact cactttcagg tatggatcgt
     1741 agacgattta ttaaaggttc aatggctatg gccgccgtgt gcggtaccag cggcattgct
     1801 tctctttttt ctcaggcggc attcgcggca gattctgata ttgccgacgg gcaaacccag
     1861 cgttttgact tctccattct acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt
     1921 ggtgcgcctc gtccgttacc tgacacgctg gcgacaatga cgccgcaggc ttataacagt
     1981 attcaatacg acgccgaaaa atcgctctgg cataacgttg agaaccgtca actggacgct
     2041 cagttcttcc atatgggaat gggattccgt cgccgcgttc gtatgttttc tgtagatcca
     2101 gcaacacatc tggcgcgtga aattcacttt cgcccggagt tgttcaaata caacgatgca
     2161 ggtgttgata ccaaacaatt agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg
     2221 tttaaagccc ccgaactggc gcgccgtgat gtagtatcat ttctcggcgc gagttatttc
     2281 cgcgccgttg atgatacata tcaatacggt ttgtcggccc gcggcctggc gatcgacact
     2341 tacaccgaca gtaaagaaga gttccccgac tttaccgcct tctggtttga tacggtaaaa
     2401 ccgggggcaa ctacctttac cgtttatgcg ttgctcgata gcgccagtat tactggtgcc
     2461 tataagttca ctatccattg tgagaaaagt caggtgatta tggatgtgga aaatcacctg
     2521 tatgcgcgca aagacattaa acagctgggc attgcgccga tgaccagtat gttcagctgc
     2581 ggtactaatg aacgtcggat gtgcgacacc attcatccgc aaattcatga ctctgatcgt
     2641 ctgtccatgt ggcggggcaa cggcgagtgg atttgccgtc cgctgaacaa tccgcaaaaa
     2701 ttgcagttca atgcttacac cgacaacaac ccgaaagggt ttggtttatt gcaactggat
     2761 cgtgacttct cccattatca ggacattatg ggctggtata acaaacgccc aagtttgtgg
     2821 gtggaaccgc gtaacaagtg gggtaagggc accatcggcc tgatggaaat cccaacaacg
     2881 ggcgaaacgc tggataacat tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt
     2941 gatgagtttg cattccagta tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca
     3001 ttagcgcgcg ttatggcgac gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca
     3061 ggtgaacact atcccgaaaa atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat
     3121 ctgaaagctg ccgcgccaaa aggcattgag ccggtgatta cgctttccag tggggaagcg
     3181 aagcaaatcg aaattctcta tattgaaccc atcgatggtt atcgtattca gtttgactgg
     3241 tatccgactt cggactctac tgatccggtc gatatgcgga tgtatctacg ttgtcagggg
     3301 gacgctatca gtgaaacatg gctgtatcag tatttcccgc cagcgccgga taaacgtcag
     3361 tatgttgacg accgcgtgat gagttaatcg ttttttcttc ggcaccttct tcgggaggtg
     3421 ccgtctggtt aaacacgatc ccgctcgcat ttttccctaa gttaaatgag taatctgatg
     3481 gtgtgtattt cagatacacc ttgtcagcca ctaacaggga gtgcgtatgt ttccagaata
     3541 ccgagattta atatcccgtc tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa
     3601 acacaataaa cttgatcatg aaattgccag aaaggaaggt tccgacggtc gagggtacaa
     3661 tgcggaagtg gtccgcatga aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat
     3721 cctgcagcag gagagcgtca aagaggtgta aactttccta agccgccagc caggcggctt
     3781 ttttaacaac agcatggatt gactggagat aagatgactg aaacgataaa agtaagcgaa
     3841 tcacttgaat tacatgctgt tgcggaaagt cacgtcacac ctctttatca gttaatctgt
     3901 aaaaataaaa cctggttaca gcagtcgcta aactggccgc agttcgttca gagtgaagag
     3961 gacacgcgaa aaacggtgca gggtaatgtg atgttgcatc aacgcggcta tgccaaaatg
     4021 ttcatgattt tcaaagaaga tgaacttatc ggcgttatct cgtttaatcg tattgaacca
     4081 ctgaataaaa ctgctgaaat cggctactgg ctggacgaat ctcatcaggg gcaggggatc
     4141 atttctcagg cgctgcaggc attgattcat cattacgctc agtctggtga acttagacgc
     4201 ttcgtgatca aatgtcgggt ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt
     4261 tttatccttg aaggttgcct gaaacaggct gagttcctga atgatgccta tgatgatgtg
     4321 aacttatacg cgcgtattat cgattcataa taaccctgcc agcggcgttc gcgtaatgcg
     4381 ctcctcgccg ttaatgactt ttggaccacg gacatgaacc gtgtcgccat caaaggcttc
     4441 aacgacggcg tggtcggtta actccacatg gttttcaatt atcaccgcac ctgaaatacg
     4501 gctctcacct tgtattacaa cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc
     4561 atttccgcca atcaagacat gatgttttaa cacacaatta ccttccacaa tggcatattc
     4621 cgccacctgc gaactgtaat gaatcgtcgg aatggcatct tcttctatgc cagctttcac
     4681 ctgcgcgtga ccgtagactt tagcgcaatc gcatagccag acgttgttct cttcattacc
     4741 ttcaacactg gcaaaatcaa aaacttcggc gcgatgttca ataaaagcat accggacgac
     4801 ggcatcgcca taaatttgtg cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc
     4861 ataaatttgg agcaggagtt gatggtcggg cgttaagcct tgtgcggcga cgatcataga
     4921 atgttgatca attaaggcgt gaccaaatat tctacattgc ccgtaaacta atgagtcacg
     4981 aatggtgaca ctgtcactta tatatgcacc ctggctgatt tcactattat cgatccagac
     5041 gttatccgtt gcgtaaacct ctccccacag cacgctggtg ccggtgatac gcgtattacc
     5101 agaaatcacc gttccgccga acgcaatagc attctggtcg taaatccagc aatttccttc
     5161 ctgtgctaac accgtttcgc gatcgatcca gccgccagcg gttcctgcta tcacatcgtt
     5221 aaaatcgcta atggcgataa tctgccgtaa taacacactt tttttagtgc catcttcctg
     5281 ataactaaag gctcgctgtt cttcacttag acgatatttg cgcatagcgt tttctcacag
     5341 gtgacttact ataaccgtag caaattctgc ggctctggct atgctcgaga aaattccata
     5401 aaatgcattt caaatatact ttataaatta aacaaaatga gtaagaagat gcagagcgat
     5461 aaagtgctca atttgccggc aggctacttt ggtattgtgt tggggacgat agggatggga
     5521 tttgcctggc gctatgccag ccaggtttgg caggtcagcc actggttagg ggatgggctg
     5581 gtgattctgg cgatgatcat ctggggatta ttaactagcg catttattac ccgactcata
     5641 cgctttccgc atagcgtgct ggcggaagtt cgccatccag tgctgagcag ttttgtgagt
     5701 ttgtttccgg caacgacgat gctggtggcg attggttttg ttccgtggtt tcgcccactg
     5761 gcggtgtgcc tgttcagttt tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact
     5821 gcgggattat ggcgcggatc tcaccctgaa gaagctacca cgcctggact gtatctgccg
     5881 acagttgcca acaactttat cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc
     5941 ggtctggtgt ttttaggcgc aggcgttttc tcatggctaa gtcttgaacc ggtgatcttg
     6001 cagcgtctgc gcagttcggg agaattaccc acggcactgc ggacatcact cggcattcag
     6061 ctcgctcctg cgctggtggc ctgtagtgcc tggttgagcg tcaacggcgg cgagggtgat
     6121 acgctggcga aaatgctttt tggttatgga ctgctgcaac tgctgtttat gctacgtctg
     6181 atgccatggt atctctccca gccatttaat gcttcattct ggagtttctc gttcggcgta
     6241 tctgcactgg caaccaccgg tttgcatctg gggagtggca gcgaaaatgg atttttccat
     6301 acgctggcgg taccgctgtt tatctttacc aattttatta ttgcaatact gctcatccgt
     6361 acttttgcgc ttctgatgca gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg
     6421 aaagcagagg acaaagaatg atcattcgtg acgaaaacta ttttactgat aaatatgaat
     6481 taacccgcac acactctgaa gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc
     6541 tggatctggg ctgtggcaat ggtcgtaaca gtctttacct ggcagccaat ggttatgatg
     6601 ttgacgcatg ggataaaaat gccatgagta tcgccaatgt agagcgcatt aaatccattg
     6661 aaaatctgga taatttacac acccgagtcg ttgatctgaa taacctcaca tttgatagac
     6721 agtacgattt tattctttcg actgtggtac tgatgttcct tgaggctaaa accatcccag
     6781 ggctgattgc caatatgcaa cgttgcacta aacctggtgg ttacaacctg attgtggcgg
     6841 cgatggatac cgctgattat ccatgtaccg tcggcttccc gtttgccttc aaagagggag
     6901 aattacgtcg atattacgaa ggctgggaga gggtgaaata caatgaagac gtcggcgagc
     6961 tgcaccgcac cgacgccaac ggtaatcgta ttaaactgcg tttcgccacg atgctggcac
     7021 gtaaaaaatg acccgctaag aataaggcgc gtgaaaattc cccacgctga gatgatttac
     7081 tgttcttctt ttcggtaagc atatttttta tcgaagggat gtgaaattaa tcacagtagt
     7141 cgaagttttt tagcagctta acttactgaa atttaactac tgatgattga cttagcccct
     7201 ttttcggcat tgactatgtc gtctgaaaag gggctgaaaa atttattttc accaacactt
     7261 tttttgccac aacacgaagc ggcgcttttt gctataactt aaaaagtaat ataatcatc
//
LOCUS       DAZPLM010000206         7147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPLM010000206 DAZPLM010000000
VERSION     DAZPLM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.206"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(596..1081)
                     /gene="sixA"
                     /locus_tag="ACHVC7_003603"
     CDS             complement(596..1081)
                     /gene="sixA"
                     /locus_tag="ACHVC7_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR2117554.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1284..3428)
                     /gene="fadJ"
                     /locus_tag="ACHVC7_003604"
     CDS             complement(1284..3428)
                     /gene="fadJ"
                     /locus_tag="ACHVC7_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR2117555.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3428..4738)
                     /gene="fadI"
                     /locus_tag="ACHVC7_003605"
     CDS             complement(3428..4738)
                     /gene="fadI"
                     /locus_tag="ACHVC7_003605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR2117556.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4918..5202)
                     /locus_tag="ACHVC7_003606"
     CDS             complement(4918..5202)
                     /locus_tag="ACHVC7_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR2117557.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5574..6914
                     /gene="fadL"
                     /locus_tag="ACHVC7_003607"
     CDS             5574..6914
                     /gene="fadL"
                     /locus_tag="ACHVC7_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR2117558.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1832 a         1871 c         1675 g         1769 t
ORIGIN      
        1 aatacggcat gcgatccaaa aagaacaggt cgtagaaaat tgtattcgtt ggtgtggggc
       61 cgcatagtga tgtttccaca cccctgatgc aatttaattt tactaaagag taaatgtaga
      121 tttaattaat atattgacag aagaaaggtt atcttaagga catccttaaa aaatacggaa
      181 taagtattta gaaaaaatag aaagtatatc aataatgaat atcaacagct taaaaaacaa
      241 acaatgaaca tataatgcga ttggcattta tccacttttg acaatataat atataaacac
      301 tatagccaga atttaatcaa gtttcaatta catcctgcct gattattcaa ctctattttc
      361 tctattatct gtattaataa atgacaatga gtgtcaaaca gacattattc atttcaacat
      421 gtaactttgg gcaaaatcaa gatacgcgca gaagaataat ttccgaatga aagtagaaag
      481 tgttttattt atagatgggg agagtcttcg atagtagtga gattgacaga tatccgctgc
      541 ctgatgagca attatgccca atgaatctac ctcattgggc ataatttgga actcatcaga
      601 tagcttttgc catcttcaga ttacacgggc tcatctgcca gttgaatgtg ccgtttccgc
      661 tttcatcaag cgttacgctg gcaatggccg aggtggtaaa cataggcggc gtttcgcccg
      721 gacataactc ggcaaccaga tacccgacta acggtaagtg agagatcacc agtaccgaag
      781 caacaccttc attggtcaac gcctgcaaat aggcactgac cagaccaaca tcgccgcagg
      841 gcgttaactc cggcagaact tctgcactgg aaggcaggtt cagacaatcc cctacttctt
      901 ccagtgtttg ctcggctcgc aggaacgggc tcaccagaac gcgttcgatt tccacttttt
      961 gacctttcag ccagttcgcc atcaggcgag attcgtcaca accattagtg gtcagaggac
     1021 gaaccgaatc actggcggca tcaagggctg cgtcgccgtg acgcatgata aaaacttgca
     1081 tattgcaccg cttttgttaa ccagattcgt ccgttttctt tcatctgaaa ccagagaaag
     1141 aaaacggtgg ccggcattgt gccttatcca ttcaccgaat gaaacgctgt tttttacctc
     1201 aatggcgtaa gtatagtcaa tatgcgttta cattttgacc aacactccgc cattcagcgc
     1261 ggattcatat agctttgacc ttcttattga aggtcagttg cagttgtttt ccaaaaactt
     1321 tccccacgcg cgcccatctc gaccaaacgc tcgcaagggg taaaacggga accatactgc
     1381 gtggccagtc gttgcattat tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg
     1441 aacggtccac cgagaaatgg cggaaaacca atgccaaata ccgcgccaat atccccgtca
     1501 cgcacgctac ggataacctg ctcatcaaca caacgtactg cttcattcag catcaacatc
     1561 acacaccgtt cagcaacctg cggtgcggag agtcgcccct gcccttgtgc gccaatgagc
     1621 gggtaaatgg cgggatcgac ctgttttttg cttttacgcc ctttctgacc ataaagatag
     1681 aaaccccgac cattttttct gcctttgcga tcgtcgttca aaattgaaga aacaacattt
     1741 gcaggcgcgc taaaacgttc tccataagcg gcttccagta caggaataat tttagtcccg
     1801 gtgtcgattc ctacctcatc caaaagttgg attgggccta ccggaaaacc aaatttcact
     1861 agcgcggcat caatgtgctc tacccgttca ccttcggtca gcatgcggat agcttcatta
     1921 atgtaaggcg ctaagatgcg attgacgtaa aacccggctt tgtcacgcac gacaatgggc
     1981 gttttgccct gttttttcgc cagttttacc gtagtggcga tggtttgcgc cgatgtaccc
     2041 gcatgaggaa taatctctac cagcggcatt ttttccacag gactgaagaa atgcaggcca
     2101 ataacttgct caggtcgcgc ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg
     2161 ttcgaagcaa aaatggtatg agcggcgcaa ttttgctcca cttccgccac catctgttgt
     2221 tttaattcga gattttcaaa caccgcttca ataatcagat cgcgatgggc aaagccgcga
     2281 tagtccgtcg ttccggagat taatgccagc tgtttgtcac gttcgctggc tttgagatga
     2341 cgacggcgaa ctttgccctc cagctgatcc caactgtact tcagcgcatg atttatgccc
     2401 tgcgggttga tatctttaat tctgaccgga agccccgctt tacaagcagt gacataagca
     2461 ataccgccgc ccatcaagcc accacctaaa attcccacgc tgtttaatgg cgcaggcggt
     2521 gcatcactac cgggatcttt cttcacgtcc gtactggcaa aaaagatact acgcaaagct
     2581 tgcgattgcg gcgtcatcgc cagttcgcca aaggcgcgag cttcagcgtc atagccgctg
     2641 ctggtgccct gcgctaatcc cgtttcaaca acctccagga tgcgttctgt cgccggataa
     2701 ttgccttgag ttttgtgttc tgttttcttg ccgaccattt tgaacagcag cgcacgacct
     2761 aacggccccg ccagaatacg ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc
     2821 ttcgccagct caacagcggc ttccagcaaa atggagtgcg gaacaacgtc atccaccagc
     2881 cccagcttta atgcctgttt cgcccgaagt tgttttccgg tgagaatcat ctctaatgct
     2941 gtactgacgc ctatcagacg cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca
     3001 agttgtactt caggcaaacc gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg
     3061 cacgccagcg ccagctccag cccaccgccc aggcaagcgc catgaatagc cgcgataacc
     3121 ggaatgggca aagcatgaat ctccgccatc aactgttggc cctgccgcgc cagtgcttcc
     3181 gcttcttgcg ccgttttgca gttgccaatc atgttgatgt ctgctccggc gataaaattg
     3241 tccggtttcg cggagataaa caccacgccg cgcaactctt tgttttcacg gagttgctta
     3301 ataatggcgc gcacctgcga ggcaaattcc gccttcaggg tattcatttt ctcatccggt
     3361 acatcgatgg tgatgatagc aatgttgtcc agacgaacat taagagtaaa cgctgatgcc
     3421 atttccatta ttccgcctcc agaaccattg ccgcgccaag cccaccggca gcacaggcgg
     3481 taactaaacc aaatccaccg ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc
     3541 gcgcgccggt cgccgcgaag ggatgcccgt aagcaatcga accgccaagc acgttaaatt
     3601 tgctatcgtc cacttcgcca gtggcatgtg cacgccccag tacttcacga gcaaaacgtt
     3661 cactacccag caactgaata ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga
     3721 tcaatgtcag ttcgctcatc gtcaaaccgg cacgttccag cgccagcggt gttgaccagg
     3781 ctggaccgag caacatgtcc tgccagacat caatcgcagt aaatgcgtag ctgcgcagat
     3841 accccagcgg caccagccct aattctttcg cccgggattc agtcatcagg atcaccgctg
     3901 ccgcgccatc ggtcagcggc gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa
     3961 acgccgggcg cagctttgcg taatcggcaa gcgaggaatt accgcgaata ttgttgtctt
     4021 cgacaagcgg ttgtttataa ggagggataa aggcagtcat cacctcttct ttgagttttc
     4081 cgtctgacca tgcctgagcg gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt
     4141 ctcgggtgat gccgtaggtt ttcgccattt gttcggcggt gtcgcccatc cgcaagccag
     4201 tagaatattc tgctaccgca ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga
     4261 agagtttcag gcgctggctc atggttcgag ctttgttgac atcaaccagc acgcgcgcca
     4321 gttttttact gacgccaatt ggcaatacgg aagaggaatc tgccccaccg gcaatccccg
     4381 ctcggatagt tcccgccatc aggctttctg cgacgtttgc aaccgcctgg aaactggtag
     4441 cgcaagcgcg gctgacgctg taagcatcgg tatgcacatt cattccggta ccgagaacaa
     4501 tttcacgcgc aatgttgggg gcttcaggca tttgtacgac ctgaccaaag accagctgtt
     4561 caatcacttc agcggggatc tcgctgcgtg ccagcagttc gcctaccacc atcttcccta
     4621 aatcaaccgc gggaatgcca tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac
     4681 cgctaacaat ggcgatacga tcgccctggc gggtaaccag cggtaaaacc tgacccataa
     4741 cactcccctg taaaaaaata aataaagtgg tctgacctga tcatagtctt aactattttt
     4801 ttacatttag ccaagcggag aaaaaggaaa gtgggagcta tgacacagtg aagacaggag
     4861 aaagaaaaga aaacgcccct gccagcaact gacaggggcg aacgtacccg agaggaatta
     4921 acgcagaccc agctggaaga tcagcatttc agcttcgcag gcaaaagtga aatcgatatc
     4981 caggcgcaca ccgtcagact cttcagtgaa agtcggggag attttgcacg gttcggattc
     5041 cacgctacgg gctttttcag tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc
     5101 aaacacgcgg ctgtaagacg cggtgcagtc ggagttgtcc ataatggtgc caacatccat
     5161 acagcagcaa accggggttt catcagcact acatttactc atcgttgatt tcctctgtat
     5221 gtgcacccaa ggtgccagat aaacgttgtg gatattttac gcttccggaa agtgctgctc
     5281 cagttgttaa ttctgcaaaa tcggataagt gaccgaaatc acacttaaaa atgatctaaa
     5341 acaaaattca cccgaatccg cgagtgcgca acctccaaat tttgccagct ggatcgcgtt
     5401 tcttagatca tatttgaaaa aagatggaaa catacttgca gcattccagc tggtccgacc
     5461 tatactctcg ccactggtct gatttctaag atgtacctca gaccctacac ttcgcgctcc
     5521 tgttacagca cgtaacatag tttgtataaa aataaatcat tgaggttatg gtcatgagcc
     5581 agaaaaccct gtttacaaag tctgctctcg cagtcgcagt ggcacttatc tccacccagg
     5641 cctggtcggc aggctttcag ttaaacgaat tttcttcctc tggcctgggc cgggcttatt
     5701 caggggaagg cgcaattgcc gatgatgcag gtaacgttag ccgtaacccc gcattgatta
     5761 ccatgtttga ccgcccgaca ttttctgcgg gtgcggttta tattgacccg gatgtgaata
     5821 tcagcggaac gtctccgtct ggtcgtagcc tgaaagccga taacatcgcg cctacggcat
     5881 gggttccgaa catgcacttt gttgcaccga ttaacgacca atttggttgg ggcgcttcta
     5941 ttacctctaa ctatggtctg gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg
     6001 ggggtacaac tgaccttgaa accatgaacc tgaacttaag cggtgcgtat cgcttaaata
     6061 atgcatggag ctttggtctt ggtttcaacg ccgtctacgc tcgcgcgaaa attgaacgtt
     6121 tcgcaggcga tctggggcag ttggttgctg gtcagattat gcaatctcct gccgggcaaa
     6181 ctcctcaagg gcaagcattg gcagctaccg ccaacggtat cgacagtaat accaaaatcg
     6241 ctcatctgaa cggtaaccag tggggctttg gctggaacgc cggaatcctg tatgaactgg
     6301 ataaaaataa ccgctatgca ctgacctacc gttctgaagt gaaaattgac ttcaaaggta
     6361 actacagcag cgatcttaat cgtgcgttta ataactacgg tttgccaatt cctaccgcga
     6421 caggtggcgc aacgcaatcg ggttatctga cgctgaacct gcctgaaatg tgggaagtgt
     6481 caggttataa ccgtgttgat ccacagtggg cgattcacta tagcctggct tacatcagct
     6541 ggagtcagtt ccagcagctg aaagcgacct caaccagtgg cgacacgctg ttccagaaac
     6601 atgaaggctt taaagatgct taccgcatcg cgttgggtac cacttattac tacgatgata
     6661 actggacctt ccgtaccggt atcgcctttg atgacagccc agttccggct cagaatcgtt
     6721 ctatctccat tccggaccag gaccgtttct ggctgagtgc aggtacgact tacgcattta
     6781 ataaagatgc ttcagtcgac gttggtgttt cttatatgca cggtcagagc gtgaaaatta
     6841 acgaaggccc ataccagttc gagtctgaag gtaaagcctg gctgttcggt actaacttta
     6901 actacgcgtt ctgataacgc gttcgcctgg ataaagtcac ctgcatagca ggtgacttta
     6961 actcccccac ttcaccgaaa gtagtgtcct cattgcttac ctcctgagtt ttgcaaacag
     7021 cctgttggca gcttgccctt tcaaatcaat aagcggtatc cacagacaaa cctgaaggga
     7081 aaggcatatt ttcaggcgtt ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc
     7141 aggatac
//
LOCUS       DAZPLM010000207         7131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPLM010000207 DAZPLM010000000
VERSION     DAZPLM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.207"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHVC7_003608"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHVC7_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHVC7_003609"
     CDS             complement(4538..4999)
                     /locus_tag="ACHVC7_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR2117559.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6929)
                     /locus_tag="ACHVC7_003610"
                     /pseudo
     CDS             complement(5027..6929)
                     /locus_tag="ACHVC7_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1316 a         2187 c         1946 g         1682 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cctggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg t
//
LOCUS       DAZPLM010000208         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPLM010000208 DAZPLM010000000
VERSION     DAZPLM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.208"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHVC7_003611"
     CDS             <1..435
                     /locus_tag="ACHVC7_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR2117560.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHVC7_003612"
     CDS             518..1420
                     /locus_tag="ACHVC7_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR2117561.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHVC7_003613"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHVC7_003613"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR2117562.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHVC7_003614"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHVC7_003614"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR2117563.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHVC7_003615"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHVC7_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR2117564.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHVC7_003616"
     CDS             complement(5330..6037)
                     /locus_tag="ACHVC7_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR2117565.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHVC7_003617"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHVC7_003617"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR2117566.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHVC7_003618"
     CDS             complement(6895..7098)
                     /locus_tag="ACHVC7_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2117567.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1855 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZPLM010000209         7097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPLM010000209 DAZPLM010000000
VERSION     DAZPLM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.209"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..485
                     /locus_tag="ACHVC7_003619"
     CDS             <1..485
                     /locus_tag="ACHVC7_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR2117568.1"
                     /translation="LLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQS
                     LEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIY
                     ALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLDK
                     "
     gene            complement(555..1526)
                     /gene="rfaC"
                     /locus_tag="ACHVC7_003620"
     CDS             complement(555..1526)
                     /gene="rfaC"
                     /locus_tag="ACHVC7_003620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR2117569.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(1530..2576)
                     /gene="rfaF"
                     /locus_tag="ACHVC7_003621"
     CDS             complement(1530..2576)
                     /gene="rfaF"
                     /locus_tag="ACHVC7_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR2117570.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2586..3518)
                     /gene="rfaD"
                     /locus_tag="ACHVC7_003622"
     CDS             complement(2586..3518)
                     /gene="rfaD"
                     /locus_tag="ACHVC7_003622"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR2117571.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3732..4928
                     /gene="kbl"
                     /locus_tag="ACHVC7_003623"
     CDS             3732..4928
                     /gene="kbl"
                     /locus_tag="ACHVC7_003623"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR2117572.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4938..5963
                     /gene="tdh"
                     /locus_tag="ACHVC7_003624"
     CDS             4938..5963
                     /gene="tdh"
                     /locus_tag="ACHVC7_003624"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR2117573.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6202..>7097
                     /gene="waaH"
                     /locus_tag="ACHVC7_003625"
     CDS             6202..>7097
                     /gene="waaH"
                     /locus_tag="ACHVC7_003625"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR2117574.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1738 a         1728 c         1791 g         1840 t
ORIGIN      
        1 cattgcttat gagaatgaat gacttaaata acgatttagt taattattcg catgataaca
       61 ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt tggcttaaaa acatattctc
      121 caataggaca atcactggaa aaacgagcgg aaaaaataca tgaactagaa gaaaaagagc
      181 ctagattgag tggcgcttta ccctatatag attctcattt gcataacgat ctcatagata
      241 cgttatcaac gcgtggtatt cctggagttg tattaacaat tttagccttt tcagcaatac
      301 tcatatatgc cttaagaact gctaaagaac cttatatttt aatcttgctt ttttcactac
      361 tggtagtagg cctaagtgat gtaatactct tttctaaacc ggttccgact gctgtgtttg
      421 tcaccataat attgctttgt gcttatttta aagcacaatc agaccaatgt ttattagata
      481 aataatgaat atgtgaaata aaatcagcaa ggaggctgat tttatttcaa aattatctat
      541 taaggggttg taatttaaat catagcagcg ttttcttcaa tgaatcgctt aacagcattt
      601 gctgtcaatt gagacaactc attccccgga gccctacaaa ccatctgatt cttcccatat
      661 ccaccaatta atcccggatc ggttggtcca taaaccgtga tattgggtct atccagtgcc
      721 gccgttaaat ggcttaaccc cgtatccacc gacactacaa atttagcccc ggccagcacg
      781 cgggcaacgc cttccagact catcttcggc aatacttcaa cataagcaaa tccttccgcc
      841 agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg gaagtttaat ccgtattcct
      901 gaatcagcca gtaaaccaat caattctcgc cagtgttctt ccggccagtg tttatcatca
      961 cgggtcgtcg catgaagaaa tacggcatat tcgccagcat ctgtaggcag gttcgtcagg
     1021 aaatgctgtg caatagcata atcgccctgg gtttgcggtt tgctatagcc cagacttttg
     1081 gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt ttgcaatatg atgcttacga
     1141 ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc agtccatgcc atgctttacg
     1201 ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca gcccctgagc gtcgataact
     1261 gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt tgcgttccgc ttttatgggg
     1321 gccgagaacc aggctttacg ccagcgacgt attgccacag gaataactcg ctcaacggcg
     1381 gcgtgccagg aaggaatctg tgcgaaccct tcttccacca cccagtcaaa cttaatccct
     1441 gggattgcct gctgggcatc agtgagtgcg ggcaacgtat ggagaacatc gcccatcgac
     1501 gatgttttaa cgatcaaaac ccgcatccgt caggcttcct cttgtaacaa tagcgcgttg
     1561 agttcttcca gtacgcgctg gggagtaatg tcgattaagc tctggtgata accctccgca
     1621 gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc ggatcacgcg cgctttatgg
     1681 gatagcggcg gtgtgaagtc cgggctactc ggaccataca gggcaaccag cggacgattg
     1741 agcgccgccg caacgtgcat caggccagaa tcgttagtga caatggcttt acaggctgca
     1801 atcaggataa ccgcttgatc aagctgtgtt tcccccgcca ggttccgaca ccatgcctgc
     1861 tgctcggtat tcaaagcggc aagaatctca ttgcccgctt catgatcttt cgccgagcca
     1921 aacagaacca cctgataacc ttcatcaatc agctgctttg ccagttccgc atagtggtag
     1981 tgtggccagc gttttgccgg accaaactcc gcacccgggc aaaaaccaat catcggacgt
     2041 tctgatgaaa gcgaaaattg attacaggta tatgattttt caccttcgct cacctgcaat
     2101 tgcggccata acaacggttt tggtagatct tgtgcggtac gcatcacgcc tttgtcatag
     2161 gccagcgcga catagcgttc caccattagc ggccaggctt ctttatcgag cacgcgtaaa
     2221 tcgttgagta aaccgtagcg catctcgccg cgccagccgg tgcgatgagg aatacccgcg
     2281 aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga cgtaggcgcg gtcgtagcgc
     2341 ttttcacgca ggctatgacc cagtttgcgg cgttcgccga tttccagcgc tccgtgaccg
     2401 agaggcatag gaatagcttc gttaacttcc ggcatccgcg ataataatgg acggcaccat
     2461 gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg cctggagcgt gcgatagaga
     2521 ctttgcgaca tcatcatgtc gccaacccaa gacgggccga tcaccagtat tttcatgcag
     2581 agctcttatg cgtcgcgatt cagccaggcc atgtattccg ttacaccttc agcaacggtt
     2641 ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca gatctgcctg agtgaacgcc
     2701 tggtagcggc ctttcagctt atccgggaac ggaatgtatt cgatctggcc tttcttgtga
     2761 taagccagcg tagcatcagc tacagcctgg aaggattccg cacgaccagt accgaggttg
     2821 aagatgccgg aaacgccatt ttccaggaac cacagattca catcagccac gtcgcccaca
     2881 tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca gcttcggtga ttcaccgttg
     2941 ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc tgcctttatg gccttcacgc
     3001 ggtccataaa cgttgaaata gcggaagcca acaatctgcg agttagcttc aggcaggatt
     3061 tggcgaacat attcatcaaa caggaatttt gagtaaccgt agacgttcaa cggtttttcg
     3121 tactcgcggg attcaataaa gtcggaggtg cgtccgccgt aggtggctgc ggaagaggca
     3181 tacaggaacg ggatttcgcg ctccagacag tagtgcagca gctctttgga gtattgatag
     3241 ttgttatcca tcatatactt gccatcccac tcggtggtgg aagagcacgc gccttcgtgg
     3301 aaaatcgctt cgacatcgcc gaactcttcg ccagccataa tctggatcag gaagtcttcc
     3361 ttatccatat agtccgcgat atccagatcc accaggttca caaacttggt gccgtctttc
     3421 aggttgtcca ccaccagaat atcggtgatg cctttatcat tcagggcctt aacgatgttg
     3481 ctgccgataa agcccgcgcc gccggtaacg atgatcataa ctgtaacctt cgaattatgg
     3541 agtcagagac aatctcagac acgaatactt ctatcatatc actacacggc gttggcttca
     3601 gccattcgcc aaaagtgaga ttgatggacg ttatttatcc tgaattttgc agaagtgtta
     3661 acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg tgctggaatt tgccctgtct
     3721 ggagaatcgc aatgcgtgga gaattttatc agcagttaac caacgatctg gaaaccgcac
     3781 gggcggaagg gttgtttaaa gaagagcgca ttattacgtc tgcgcagcaa gcagatatca
     3841 ctgtggctga tggaagccac gtcattaact tttgtgccaa taactatctc gggctggcga
     3901 attatccgga tctgattgcg gcggcaaagg cgggaatgga ttctcacggt ttcggcatgg
     3961 cttcggtgcg ttttatttgc ggcactcagg acagccataa agagcttgaa caaaaactgg
     4021 cggcattcct ggggatggaa gatgcgattc tctactcttc ctgctttgat gctaacggtg
     4081 gcctgtttga aacgctgctg ggcgcggaag acgccattat ctccgacgca ctgaaccacg
     4141 cgtctattat tgatggtgtg cgtctgtgta aagctaaacg ctatcgctat gccaacaacg
     4201 atatgcagga gctggaagca cgtctgaaag aagcgcgtga agccggtgcg cgtcatgtgc
     4261 tgattgccac cgatggtgtg ttctcgatgg acggcgtgat tgccaacctg aagggcgttt
     4321 gcgatctcgc agataaatat gatgccctgg tgatggtaga cgactcccac gcggtcggtt
     4381 ttgtcggtga aaatggtcgt ggttcccatg aatactgcga tgtgatgggc cgggtcgata
     4441 ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc tggtggttat accgcggcgc
     4501 gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc gtacctgttt tccaactcgc
     4561 tggcaccggc cattgttgcc gcatccatca aagtactgga gatggtcgaa gcgggtagcg
     4621 agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg tgagcaaatg tcggcggcgg
     4681 gctttaccct ggcgggagcc gatcacgcca ttattccggt catgcttggt gatgcggtag
     4741 tggcgcagaa atttgcccgt gagctgcaaa aagagggcat ttacgtcacc ggtttcttct
     4801 atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc
     4861 ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta
     4921 ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa agcggaagag ggcatctgga
     4981 tgaccgacgt tcctgtaccg gaactcgggc ataacgatct gctgattaaa atccgtaaaa
     5041 cagccatctg cgggactgac gttcacatct ataactggga tgagtggtcg caaaaaacca
     5101 tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga agtggtaggt attggtcagg
     5161 aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga aggccatatc acctgcggtc
     5221 actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg caacacgatc ggcgtcggcg
     5281 ttaaccgccc gggctgcttc gccgaatatc tggtgattcc ggcgttcaac gccttcaaaa
     5341 ttcccgacaa tatctccgac gacctggctt ccatttttga tcccttcggt aacgccgtgc
     5401 ataccgcgct gtcgttcgat ctggttggcg aggatgtgct ggtctctggt gcaggcccga
     5461 ttggtattat ggcagcggcg gtggcgaaac acgttggtgc acgcaatgtg gtgatcactg
     5521 atgttaacga atatcgtctc gaactggcgc gcaaaatggg tatcacccgt gcggtcaacg
     5581 tcgctaaaga aaaccttaat gatgtgatgg cagaactggg catgaccgaa ggctttgatg
     5641 tcggtctgga aatgtccggt gcgccgccag cgtttcgtac catgctcgac accatgaacc
     5701 acggcggtcg cattgcgatg ctgggtattc caccgtccga tatgtctatc gactggacca
     5761 aagtaatctt taaaggcttg ttcattaaag gtatttacgg tcgtgagatg tttgaaacct
     5821 ggtacaagat ggcggcgctg attcagtcag gtctggatct ctcgccgatc attacccatc
     5881 gtttctctat cgatgatttc cagaagggct ttgacgctat gcgttcgggc cagtccggga
     5941 aagtaattct gagctgggat taacacgaac aagggctggt attccagccc ttttgcctga
     6001 ggataatccg ttaaatatgt aaaatcctgt cagtgtaata aagatatcgt aattgcgctg
     6061 atctcttata tagctgctct cattatctct ctaccctgaa gtgactctct cacctgtaaa
     6121 aataatatct cacaggctta atagtttctt aatacaaagc ctgtaaaacg tcaggataac
     6181 ttcagaggtc gtcggtaatt tatgatgaac agcaccaata aacttagtgt tattattccg
     6241 ttatataatg cgggcgatga tttccgcact tgtatggaat ctttaattac gcaaacctgg
     6301 actgctctgg aaatcattat tattaacgat ggttcaacgg ataattctgt tgaaatagca
     6361 aagcattacg cagaaaacta tccgcacgtt cgtttgttgc atcaggcgaa tgctggcgca
     6421 tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca aatatgtcgc ttttgtcgat
     6481 gctgacgatg aagtctatcc caccatgtac gaaacgctga tgaccatggc gttagaggac
     6541 gacctcgacg tggcgcagtg caacgctgac tggtgttttc gtgaaacggg agaaacctgg
     6601 aaatccatcc ccagcgatcg ccttcgctca accggcgtat taaccggccc ggactggctg
     6661 cggatggggc tttcttcgcg ccgttggact cacgttgtct ggatgggggt ttatcgccgc
     6721 gatgttattg ttaaaaataa cattaaattt attgccggat tacatcatca ggatattgtc
     6781 tggacaacag aatttatgtt taatgcgctg cgtgcgcgat ataccgagca atcgttatat
     6841 aaatattatc tgcataatac gtcagtgagt cggttgcata gacaagggaa caaaaacctt
     6901 aattatcaac gtcactatat taagattact cgtctgctag agaaattaaa tcgaaattat
     6961 gccgacaaaa ttacgattta tccggaattt catcagcaaa taacttacga agcattgcgt
     7021 gtttgccatg cggtgcgcaa agagccggat attattaccc gccaacggat gattgccgag
     7081 atatttactt ccggtat
//
LOCUS       DAZPLM010000210         6856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPLM010000210 DAZPLM010000000
VERSION     DAZPLM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.210"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHVC7_003626"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHVC7_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR2117575.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHVC7_003627"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHVC7_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR2117576.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHVC7_003628"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHVC7_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR2117577.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHVC7_003629"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHVC7_003629"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR2117578.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHVC7_003630"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHVC7_003630"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR2117579.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..6855)
                     /locus_tag="ACHVC7_003631"
     CDS             complement(4156..6855)
                     /locus_tag="ACHVC7_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFR2117580.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1661 a         1863 c         1624 g         1708 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa
     6841 aggatattta cgcata
//
LOCUS       DAZPLM010000211         6825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPLM010000211 DAZPLM010000000
VERSION     DAZPLM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.211"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHVC7_003632"
     CDS             complement(155..667)
                     /locus_tag="ACHVC7_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR2117581.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHVC7_003633"
     CDS             862..1626
                     /locus_tag="ACHVC7_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117582.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHVC7_003634"
     CDS             1577..2980
                     /locus_tag="ACHVC7_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR2117583.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHVC7_003635"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHVC7_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHVC7_003636"
     CDS             4337..5080
                     /locus_tag="ACHVC7_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR2117584.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHVC7_003637"
     CDS             5084..6304
                     /locus_tag="ACHVC7_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR2117585.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHVC7_003638"
     CDS             6308..6814
                     /locus_tag="ACHVC7_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117586.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1815 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacga
//
LOCUS       DAZPLM010000212         6773 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPLM010000212 DAZPLM010000000
VERSION     DAZPLM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.212"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..274)
                     /locus_tag="ACHVC7_003639"
     CDS             complement(<1..274)
                     /locus_tag="ACHVC7_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFR2117587.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFA"
     gene            complement(531..1166)
                     /gene="msrQ"
                     /locus_tag="ACHVC7_003640"
     CDS             complement(531..1166)
                     /gene="msrQ"
                     /locus_tag="ACHVC7_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR2117588.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(1167..2171)
                     /gene="msrP"
                     /locus_tag="ACHVC7_003641"
     CDS             complement(1167..2171)
                     /gene="msrP"
                     /locus_tag="ACHVC7_003641"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR2117589.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2280..2693)
                     /gene="hiuH"
                     /locus_tag="ACHVC7_003642"
     CDS             complement(2280..2693)
                     /gene="hiuH"
                     /locus_tag="ACHVC7_003642"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR2117590.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2826..3497
                     /gene="hprR"
                     /locus_tag="ACHVC7_003643"
     CDS             2826..3497
                     /gene="hprR"
                     /locus_tag="ACHVC7_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR2117591.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3497..4855
                     /gene="hprS"
                     /locus_tag="ACHVC7_003644"
     CDS             3497..4855
                     /gene="hprS"
                     /locus_tag="ACHVC7_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR2117592.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(4963..5814)
                     /gene="hchA"
                     /locus_tag="ACHVC7_003645"
     CDS             complement(4963..5814)
                     /gene="hchA"
                     /locus_tag="ACHVC7_003645"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR2117593.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            5961..6371
                     /locus_tag="ACHVC7_003646"
     CDS             5961..6371
                     /locus_tag="ACHVC7_003646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117594.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(6405..>6773)
                     /locus_tag="ACHVC7_003647"
     CDS             complement(6405..>6773)
                     /locus_tag="ACHVC7_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2117595.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT         1874 a         1410 c         1603 g         1886 t
ORIGIN      
        1 cagcaaatgt tttaatttta tctgcatccg ctttcttctg aaagacgggg tcaagtttgc
       61 cactttgcag taaaggataa acggattgcc agactccatc ccagtcactg agcgttcggt
      121 tttgtacatt ggcatcatca aaaacaccat tagcagcttt ttgttcgacc tctgttaagg
      181 gtttgccgtg tgagtgatga ccatgcgaaa aggcaggagc gctaacaata aagacaccta
      241 aagcaacagc cagtttgtga agacgaattg ccaaaacagt ttcctccaga gtcattgtat
      301 atgatgtgta atgttatatt gtaacatatg gcaaaatgca acaactctta ggagatgaga
      361 gcatagtttt ctcaggtgta cttatctatc ttattgaaat aaaatgaatt atgttgataa
      421 tttgaggttg atgacaaacg aaacataacc tgatgtgatc tgcgcaggcc ggacagggcg
      481 ctcgcgccac atccggcagg gataacgcgt actttgtcgg caatatgaag ttatacagat
      541 aatttgttgt gaacctgctt gcgtaaccgc ttaaaaaggg aacgcaactt cttataccgt
      601 aaggctaaaa gcagtacagc cagcccagcg tagatgagcg gctgcggtga aataatcttc
      661 actgaccaca gataatgtat tggggccagg atcgcgacaa gatagacgaa attatgcaac
      721 tgttgccagt gcttgccgag ttttcgctgc atcgcctggg ttgaagtaaa cgctaaagca
      781 agcaaaataa cccagctgat aattcctaac gttaaataag gccgggtaat taactccttg
      841 cctaataacg ccagattatt cacgcccagc tccagcaatg cgtaactggt taaatgcagt
      901 gtcgcccagg caaagcacca taagcctaac aggcggcgag tgcgtatcaa taacggctgt
      961 tttgcgtagc gtgccagagg tgtgattaac aaggtcgcca gcaaaaattt cagtgcagtg
     1021 cgaccggtaa aatgctgaat atctttcacc ggatcggcac ccagtccacc gtgattgatc
     1081 gcccagacca gccagagaaa cggcaacaat ccggcaagat gcaggctaac tttcagccat
     1141 gtcacctgtt ttgctgtcag acgcattcag aaattctccc gcaaatccag gccacgatac
     1201 agcgatgcca cctggtcggc gtaaccatta aacagtagcg ttggctggcg ctgaacatcg
     1261 aggatgccgc ctgaaccaat aaatcgttcg gtagcctgtg accagcgcgg gtgatcaaca
     1321 tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg ctgccagatt ccaggtggtt
     1381 ggcggacgct cgcgggtcag cttaatactg actatcgatt taatcccttt aaagccatat
     1441 ttccacggca caatcagtcg taccggcgcg ccattttgtg gcggtaacgc cttgccataa
     1501 acacccacgg tcatcagtgt gagcggatgc attgcttcgt cgagacgcaa tccttcgaca
     1561 taaggatatt tcagcccgcc gccgataaag cggtcctgct ggcctggcat ttgttccggt
     1621 gcataaattg tttcgaaagc gacatacttc gcattgctgg tgggttcggc aagcgccagc
     1681 aatttgtgca gcggaaaacc aatccacggc accaccatcg accatgcctc cacgcagcgc
     1741 atacgataaa tacgctcttc cagcgggaag cgacgggtta aatcatcgtg atcgagggtc
     1801 aatggttttg ccacttcgcc gctgattttc agtgtccatg gatcggtttt caggctacca
     1861 gcattagcgg cgggatcggc tttatccagc ccgaattcat agaagttgtt ataaccggag
     1921 actttatcta ctggcgtcag tggcagatta ttttgccagg cggtaggctt gctgaactcc
     1981 agcggttttc cggcgggggc gggcgggcga tcgttccctt taaaccagct aagcagatcg
     2041 gcatgcgcag cgtgaggcaa agaaagtgca gctgcgctga tgcccagtgc ttttaacacc
     2101 tgtcgacgct tcataaagaa taccgactcg gccgtaacat ctgattcttt taaaaattga
     2161 ttctttttca taagccatca cacttttttg tcgttagcca gcaagagccg tttactgtta
     2221 atctgtcagg gagtgtcctc cctaacagta gcagagatct gcttttacat atttatgcat
     2281 taactgccac gataggttga atacccatat tggctaagta ataaaggcac atgataatgc
     2341 tcgttcactt tattaatatg aaattcaacg gggatctcag ggaagaaact ttcaagattt
     2401 tgtttcttga aatagtcccc ggttttaaat acgacacggt aatcgcccgt agttgcagtt
     2461 tgctcgggcc acagtgcctt aattcgtcca tccttatctg ttttggcggt attaagttgt
     2521 aaccagccgt tgtccgcttt cttttcaaga gtgactgtca cgtcggcagc gggttttccg
     2581 gtttgctggt tcaaaatgtg cacgctaaga atgttttgtt gtgcggcata aaccaaagaa
     2641 ggtaatgaaa atgctgccgt tactacggag agtactaaat aacgctttaa catgtttata
     2701 tccttgtcat gtgaatgagt tttgaggata gcgaaaaagc ggccagggta accttacagc
     2761 agcattacaa ttttgtaatg aactaggctg tttctataac atatgattta tggcatatta
     2821 ttttcatgaa gattttactt attgaagata atcaaaggac ccaggagtgg gtaacgcagg
     2881 ggctttccga agcgggttat gtcattgatg ccgtttctga tggcagagat gggctttatc
     2941 ttgcgctgaa ggatgattat gcattgatca ttctggatat tatgcttccg ggtatggatg
     3001 gctggcagat cttacaaacg ttaagaacag caaagcaaac ccctgttatt tgccttactg
     3061 caagggattc tgtcgatgac agagtcagag ggctggacag tggagcaaat gattatctgg
     3121 taaaaccttt ttcattttct gagttgctgg caagggttcg ggcacaatta aggcaacatc
     3181 acgctttgaa ttcaacatta gaaatcagtg gcttaagaat ggactctgta agtcaaagtg
     3241 tgagcaggaa caatatcagt attacactga cgcgcaagga gtttcagtta ctttggctac
     3301 tggcctccag agctggcgaa attataccca gaacggttat tgcgagtgaa atttggggaa
     3361 tcaactttga tagtgatacc aatacggtgg atgtcgccat tcgcaggctc cgcgcaaaag
     3421 ttgatgatcc ttttcctgaa aagctaatcg ccacaatccg ggggatgggc tattcattcg
     3481 tagcggtaaa aaaataatga aaagattatc tataaccgtc cgtttaacct tgctttttat
     3541 attgctgctg tctgttgctg gcgctggaat tgtctggact ctctataatg gcctggcaag
     3601 tgagttgaaa tggcgcgatg atacaacact cattaaccgg acagcacaga tcaagcagtt
     3661 gttaattgat ggggtaaatc cagatacgtt acctgtatac tttaaccgga tgatggatgt
     3721 tagtcaggat atcttgatca ttcatggtga tggcatcaat aaaattgtta accggacaaa
     3781 tgtcagtgat gacatgttaa ataacatacc tgctagtgag acaatcagcg cagctggcat
     3841 ttacagaagc attattaatg atactgagat tgatgcttta cgaattaata ttgatgaagt
     3901 ttcgccatca ttaacggtta ctgtggctaa attggcttca gccagacata acatgcttga
     3961 acagtataaa attaatagca ttataatttg cattgtcgcc attgtacttt gctcagtatt
     4021 aagtccgctg ttaatcagaa cgggattacg agagatcaaa aagttgagtg gtgtaacgga
     4081 agcgctgaat tataacgata gccgggagcc tgttgaggtt agcgcattac cgagagaact
     4141 aaaacctctt gggcaggcgt tgaataaaat gcatcatgct ttagtcaaag attttgagcg
     4201 tctaagtcaa tttgctgacg atctcgctca tgaacttaga acgcccatta atgcattact
     4261 gggtcagaat caggttacgc tcagtcaaac cagaagtatc gctgaatatc aaaaaacaat
     4321 tgccggtaac attgaagagc tggaaaatat ttcgcggtta acagagaaca tactgtttct
     4381 tgcccgggca gataaaaaca atgttttggt gaaactggac tcgctttctc tcaataagga
     4441 agtcgaaaat ttgttggatt atcttgaata cctttcagac gagaaagaga tttgctttaa
     4501 ggtcgagtgc aatcagcaaa tctttgcgga taaaatttta ctgcaacgaa tgttatcgaa
     4561 tcttattgtt aatgccatta gatattcacc agaaaaatcg cgtattcata taaccagttt
     4621 tcttgatacc aacggctatc ttaatattga tgtcgccagt cctggaacga aaattcatga
     4681 gcctgaaaaa ctcttccgta gattttggcg gggagataat tcgcgtcatt ccgtaggtca
     4741 gggacttggt ctttctttag tcaaagcgat tgccgaatta catgggggaa gtgctacgta
     4801 tcactatctc aataagcata atgtgttccg gattatgtta ccgcaaagaa attaacatag
     4861 ggcttcagta cgccataaat caaccatgaa aaaacgcagc cacgtcgtga gtattaacgt
     4921 ggctgcgatt gattatgcgt ttacattcaa acgtaacagg gattaatccg cataagctgc
     4981 cagcatttcc tgcgccgcca gtttacccaa cgcattcgct gcgaaaggac tgtcgccggt
     5041 gagaacttta cggtccttat gtactcgccc ggtgatgtcg tcattaataa tgttcatgcc
     5101 cattttcttc agctcttcgc cgaagtacca ggtgagatga cccggcatat agcctatctc
     5161 tggcgtttgt ttgtctgcgg cgtctgggaa tgcgcaaatg gaataaccat tcagtgggtt
     5221 atcgccgtgg cgaagcgcca gaaaagccgc cgggccgtgg caaagggaga taacaaaacg
     5281 gtcatttttg attgcccact gcaaagcagc agctacgtcc tggctttctg gtaaaccaat
     5341 aagtgcgcca tgaccaccag gaacaaagat tgctgcatat tcgctatcag cgttgaggct
     5401 ggcgacaaca tccgcgagtt tcttcggatt gcggaacaac gatttatgct gctcaaagaa
     5461 tggcattact ttttcatctt tgtgcggcat agcccagtat tcaaacttgg tcatcagacc
     5521 ggaaatggtc gccacttcga attcgaaacc agcagcatgg agatgataca acggcagcaa
     5581 cgtttcaatc ggatggttac cggtcgagaa cagttttccg ttatcggttg gcaaataacg
     5641 ttcgtccgcg gcgatcacca gaattttatg cttaccgcga tacggttttg gatagtccac
     5701 gccatcaaga tcagagacgg gactggtata ttggctaagc gaatattctg aagggaagaa
     5761 tgcattatct tcagcaatat cgacctgcgg atttttactt gtttgaacag tcatagtgta
     5821 ttccttattg ttgcttagtt agggtagtca ctatatttgc gactgagctg gtcgctattt
     5881 ttaaccagtc tggaaaattg agtcaatata tcgaaagcgg tagtcggcgg gtgtaagtat
     5941 gtaatgatga tgctgcactg gtgttgaata ttattcacgc caaattttcc ttattgagca
     6001 acgtcgtcgc aagtggcctc aatgatttta gaccattatc aaaggtacgg ggcggcgggg
     6061 agagatttag tgctgtgaaa tattgtggta ctgatgaata tttatatcgc ggattggtag
     6121 agaaaagatt atttcatgca ctatttttgc aaatatttct atatgggaag cgtgtttgtt
     6181 acggattaat tcataaagaa atgcatttgg gtgttggttt gaagagtcgc tggttgatgt
     6241 tattgtgcca ggaggggcag ggtaaggtgc tgtatcgata tgcattggca attgcgtgtg
     6301 ccgcagatgg agatgaaatg ccaataaaaa agcctgaatt cgatgaggga cgaactcagg
     6361 ctagtgttta gttttacagt aacacgccgc agcacgttgg ctgattagaa ctggtagact
     6421 aagcccacac caacaacgtc atctgttgcg atgccattgt tgtcatagaa atcgtcatct
     6481 ttatccaaca agtttatttt atagtcaaca tatgttgaga agttcttatt gaaatagtag
     6541 gtcgctccaa tttcaacata gttgaccaga tcctgatcaa catactcttg atgaccattg
     6601 ttgttatagt cgttaccgcc gaggtctttg ccttttgaat aaacatatgc aagagatggg
     6661 cgcagaccaa aatcgaactg atactgtgct acagcttcaa agttctgggt tttatttgcg
     6721 atacccacat taccatatgg tgtcatattg cgggtttcag catacatcat tgc
//
LOCUS       DAZPLM010000213         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPLM010000213 DAZPLM010000000
VERSION     DAZPLM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.213"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHVC7_003648"
     CDS             complement(<1..1094)
                     /locus_tag="ACHVC7_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR2117596.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHVC7_003649"
     CDS             complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHVC7_003649"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR2117597.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHVC7_003650"
     CDS             complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHVC7_003650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR2117598.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4062..4970
                     /locus_tag="ACHVC7_003651"
     CDS             4062..4970
                     /locus_tag="ACHVC7_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2117599.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            5300..5863
                     /gene="smrA"
                     /locus_tag="ACHVC7_003652"
     CDS             5300..5863
                     /gene="smrA"
                     /locus_tag="ACHVC7_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR2117600.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQVKQENWERHAKRSR"
     gene            complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHVC7_003653"
     CDS             complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHVC7_003653"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR2117601.1"
                     /translation="FYARFFLTNSAPMLLIDPSRDGQIVDANLAALNFYGYNHETMCQ
                     KHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQTYAGPIEIYGDKL
                     MLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLL
                     IDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDT
                     ALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAKNDGRN
                     RVLAA"
BASE COUNT         1685 a         1715 c         1704 g         1626 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtgg catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag ccgccttctt cgccaggaca accataaaag
     2161 cgcactgtgc cgccttgccc atattgttcc agccatttct tgacggctat tgcagcggca
     2221 aaggcggcgg ttccccgcaa attgtgtccg caaccgtgac cattttcacc gggcgtcgcg
     2281 gatgtaggtt gcgcgcaacc agcttgctga cttaaacctg ccagggcgtc atattctccc
     2341 agcagggcga taaccggttt gccttgacca aacgaagcaa taaaggcatt tgggatattg
     2401 cctacgttgc gggtaacggt gaagcctgca gattccagag ccgaagccag atgctccgct
     2461 gaccagaact cttcaaaacg tgtttctgga tgatcccaga tttgatcggc aatatcggta
     2521 taacgttggc gatcggcttc aatcgcatcg tcgataaaac gatagatttc ctgcatcaga
     2581 tacctcgcgt ccagggaaaa ttgagcgcgg tgcgcgccag cgtttcgacg gcaatagcga
     2641 gaacctgctc gtcaaaatcg aatttttcgt tgtgatgacc tgccgccagc tgtgtgccaa
     2701 acaccacgta ggaggcttgc ccttgatgtt gctgcacgcg ggccatcatt aatgtggcat
     2761 cttcggaacc cgcaggcgct tcaacacgtt caatggcctg attgaccccc gcgacctgag
     2821 ccgcctgact ttgcaaccat gcgacccatt gcggcgaagg agaactggcg gtagctgcac
     2881 ccatcagacg agtttcaacg ccgacaccat acatggttgc tgcgccctga atcgcttgtt
     2941 gtgcacggtc aaaaacatat tgattaatga cgtcgctggc cccgcgtgtt tccactttca
     3001 gcaacgccga ggcaggaaca acgttacgac cgcttcctgc ctgcataacg cccacgttta
     3061 ctctggaagc tccttcgctg tgcggggcga ttgcatgcag tgcaagagtg gcttgtgctg
     3121 ccgccaacaa ggcattgtga ccgtcttctg gttttgcgcc tgcgtgagcg gcggtaccgg
     3181 tgaagtgcgc gtcaaatttg gtggttgcca taaaattatc actgccgcac accacggtgc
     3241 ccgcaggtac gccagtgcca atgtgcacgg cagtaaaata atcaacatca tctacgacac
     3301 ctgcatcgac catcgcccgc gcgccacgcg taccttcctc tgcaggctga aaaatcagtt
     3361 tgatgacgcc atgtagtccg gactcgaatt gtttaagggt atgcgccagc ccaagcccaa
     3421 tggcggtatg tccatcatga ccacaggcat gcatcattcc ggcgttacat gacgcaaaac
     3481 cgtcgcggta ggggcgatgg ctgacatcct gctcttcact gagatccagc gcgtccatat
     3541 cgacacggaa agccatcacc ggaccggggc gaccggtatc cagggtggcg acgataccag
     3601 tgaaaccacc ttcaaaaacc gcaatccatt gtgctagcgc accctgttga cgagcgcgct
     3661 cgaattcgcg ttgtagagtg agttcatcag gtagtcccat ccggctactt tcattaacta
     3721 cttcgcggcc cagcgccagt gaatagccga gctgctgcaa ttcttccgca acaagggtgg
     3781 cagtgcggaa ttccacccag ccagactctg catagtgatg aaaatcacgt cgccagtgcg
     3841 ataattttgg ggcaagcgaa ttaacaaatt gattcaaaga ctccataacc tttcccgtca
     3901 tcagtaaaaa gtgtgacccg gttcacgtag cgatagtttt tacttatcac taactgattt
     3961 ttcacagttt taaccgttca taaattaccc tgacacaatc atctgcatta aagtagatgc
     4021 cagtttcttt ggtctgataa ataacggtta tcggtggcgt catggctttt caggtaaaaa
     4081 ttcatcaaat tcgggctttt gttgaagtgg ctcgtcaggg cagcattcgc ggagcgagcc
     4141 gaatgttgaa tatgtcgcaa ccggcactga gtaaatctat tcaggagctg gaagaaggat
     4201 tagcggcgca actctttttt cgccgtagta aaggcgtgac gttaactgat gccggtgaaa
     4261 gtttttatca gcacgccagt cttattcttg aagagctgcg cgcagcccaa gaggatattc
     4321 gccaacgaca agggcaactg gcagggcaga ttaatatcgg catgggggcc agtatttccc
     4381 gcagtctgat gccagctgtc atatctcgtt ttcatcagca gcatccgcag gtaaaagtac
     4441 gcattatgga agggcaactg gtgtcgatga ttaatgaatt gcgtcaggga gaattggatt
     4501 tcaccatcaa tacctattat cagggaccgt acgaccacga atttactttt gagaaattac
     4561 tggaaaagca attcgcgatc ttttgccgcc cgggacaccc cgccattggt gcccgttcga
     4621 tcaaacagtt actggattac agctggacaa tgccgacgcc acacggcagt tactacaaac
     4681 agttgagtga attgcttgac gatcaggcgc aaacgccaca ggtcggtgta gtctgcgaga
     4741 cgttctcagc ctgtatcagt ctggtggcaa aaagcgattt tctcagcata ctgcctgaag
     4801 aaatgggctg cgatcccttg cacggacagg ggctggtgat gttgccggtt agcgaaattt
     4861 taccgaaagc ggcctattat ttgattcagc ggcgtgatag tcgccagaca ccactgaccg
     4921 cgtcattaat cacgcaattc cggcgagaat gcggctatct gcaaagttaa aactgcataa
     4981 aaaaatagag tctgtcgaca tccgccagac tctacagtac acacagcagt gcatccgcgt
     5041 cttaaatccg gcattgtctc ctctgcgccg gtgactgtgt tataacggtt ttaaccttta
     5101 gttgccaatt ttcttcagtg acagatttca cgaaaatata agttacatta ttaatatcgt
     5161 gaatgaataa tcatgcataa gcattttgct taaaatatcg gcaatatttg gaacttatta
     5221 ctggaatttt gggtaatacg ttgttggacc gacccggtct ggttatcata tcgcgctctt
     5281 aattgcggga ggatgtaaca tgaaccttga cgacaaatcg ctgtttcttg acgccatgga
     5341 agatgtccag ccgctgaaac gtgctaccga tgtccactgg catccaacgc gtaaccaacg
     5401 tgcgccgcag cgtatcgaca cgctgcagct tgataatttc ctcaccaccg gatttctcga
     5461 catcatccca ctaagtcagc cgctggagtt tcggcgggaa gggttgcaac atggggtgct
     5521 ggataagctg cgcagtggta aatatccgca acaggcgagc ctgaatcttt tgcgccagcc
     5581 ggtggaagag tgccgcaaaa tgatgttcag tttcattcaa caagccatgg ctgatggttt
     5641 gcgtaacgtg ctgattattc atggcaaagg acgggatgat aaatcgcatg ccaatattgt
     5701 ccgcagctat gtggcgcgct ggctgaccga atttgatgat gttcaggcat attgcactgc
     5761 gctaccgcat catggcggca gcggggcgtg ttacgtcgca ctacgtaaaa cggcgcaggt
     5821 gaagcaagaa aactgggagc gccacgctaa gcgcagtcgt tgatctcgag acgcatccgc
     5881 ggcttatgcc gccagcacgc ggttgcgtcc atcatttttc gcccgataca aagcatcatc
     5941 aacgcgttta aacagttcat cgatgctttc atttccttcg tgatgcgcca caccaatgct
     6001 gacggtaaag cgtggtaagc ccgaaatact cacttttgcc acgcttacgc ggatagtttc
     6061 agccagcgaa agcgcggtat ccagtggggt tcttggtagc aataagacaa actcttcgcc
     6121 tccccaacga aacaccaaat cgcctttgcg agcgcaactt tcgagggtgc gggcgagggc
     6181 gcataacacc tcatcacctt tagaatgccc atagagatcg ttaatgtgtt taaaacgatc
     6241 ggtgtcgatg agcaacaagc tgtaatcttg ggcgatggcg agatgctgca tttggcctgg
     6301 ctcggtaatg tgataaaact gtcgccgatt cagtaatccg gtcatcgcgt catggtgggc
     6361 agcatgttcc agctgctcct ccagccgttt ttgctcagta atatcatgta caatacataa
     6421 catgagcttg tcgccataaa tttcaatcgg cccggcgtag gtctgcacat gacgagtcga
     6481 accatccgcc agcttatgaa taaaattcag aggtttatga ccaccgggta aatgcgaaat
     6541 ttcatgcatg ataggcatga cgcgacgccc gagcatattt atttcccagg tatgtttctg
     6601 gcacatcgtt tcatggttat aaccatagaa attgagcgcg gcgaggttag catcgacgat
     6661 ttggccatct cgtgaggggt caatcaacaa cattggtgca gagttagtca gaaaaaagcg
     6721 cgcataaaaa
//
LOCUS       DAZPLM010000214         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPLM010000214 DAZPLM010000000
VERSION     DAZPLM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.214"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHVC7_003654"
     CDS             38..250
                     /locus_tag="ACHVC7_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117602.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHVC7_003655"
     CDS             283..501
                     /locus_tag="ACHVC7_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR2117603.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHVC7_003656"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHVC7_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHVC7_003657"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHVC7_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR2117604.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHVC7_003658"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHVC7_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHVC7_003659"
     CDS             1917..2660
                     /locus_tag="ACHVC7_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR2117605.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHVC7_003660"
     CDS             2690..3229
                     /locus_tag="ACHVC7_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR2117606.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHVC7_003661"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHVC7_003661"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR2117607.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHVC7_003662"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHVC7_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR2117608.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHVC7_003663"
     CDS             4582..6102
                     /locus_tag="ACHVC7_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2117609.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6730)
                     /locus_tag="ACHVC7_003664"
     CDS             complement(6135..>6730)
                     /locus_tag="ACHVC7_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR2117610.1"
                     /translation="RAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQ
                     IITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATGK
                     TATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVK
                     NGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1774 a         1538 c         1589 g         1829 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct
//
LOCUS       DAZPLM010000215         6691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPLM010000215 DAZPLM010000000
VERSION     DAZPLM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.215"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(76..345)
                     /locus_tag="ACHVC7_003665"
     CDS             complement(76..345)
                     /locus_tag="ACHVC7_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2117611.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            402..1070
                     /locus_tag="ACHVC7_003666"
     CDS             402..1070
                     /locus_tag="ACHVC7_003666"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2117612.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(1015..1152)
                     /locus_tag="ACHVC7_003667"
     CDS             complement(1015..1152)
                     /locus_tag="ACHVC7_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR2117613.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1125..1223)
                     /locus_tag="ACHVC7_003668"
                     /pseudo
     CDS             complement(1125..1223)
                     /locus_tag="ACHVC7_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1212..1451)
                     /locus_tag="ACHVC7_003669"
                     /pseudo
     CDS             complement(1212..1451)
                     /locus_tag="ACHVC7_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1550..2440
                     /locus_tag="ACHVC7_003670"
                     /pseudo
     CDS             1550..2440
                     /locus_tag="ACHVC7_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2443..2976
                     /locus_tag="ACHVC7_003671"
     CDS             2443..2976
                     /locus_tag="ACHVC7_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2117614.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(3005..3532)
                     /locus_tag="ACHVC7_003672"
     CDS             complement(3005..3532)
                     /locus_tag="ACHVC7_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2117615.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3534..6290)
                     /locus_tag="ACHVC7_003673"
     CDS             complement(3534..6290)
                     /locus_tag="ACHVC7_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2117616.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQET
                     VNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            complement(6442..>6691)
                     /locus_tag="ACHVC7_003674"
     CDS             complement(6442..>6691)
                     /locus_tag="ACHVC7_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR2117617.1"
                     /translation="GVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWG
                     AGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
BASE COUNT         1647 a         1655 c         1546 g         1843 t
ORIGIN      
        1 gctctgacaa cgatgttaac ccgcgttaaa tatcccacgt tggccagcat ctgtctcacg
       61 gaaagtcata ctgccttaca ttgtgtctgg ttcttccggc cactcaatat caggtgcagt
      121 tgatgtatca acacggttca gcaacacccg atacttcttc caggcttcca gcaacgagtt
      181 ttcttcctcc gttgcgatct ccagatctac agcatcctga agtggcgcaa tatgctcact
      241 gaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      301 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      361 tcatcgccat aattgattta attcacaaat aaaactataa tatggtgaaa ttaatgaaaa
      421 aaaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt tatgactggt
      481 gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa caccttcttc
      541 cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga actgggtttt
      601 accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac gaagctagcc
      661 tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt agccatgatg
      721 tttttgaacc ttatcccgcg gcgttacatg gtcaatttga ttccatttcc atgttttacc
      781 ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc aatgcggcgc
      841 aggccttaac tgatgatgga actctatacg gagccacaat tcttggcgat ggagttgtgc
      901 acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca
      961 caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag aatgttaaaa
     1021 ccagggttca aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg
     1081 cagcacgttc ttgcttaaga cgtgctgcgg cataatccca atgattactc cctgacaggg
     1141 ttcgtaggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga
     1201 tactttttct aggcttccac caccagcacg acaagatgcc gcatacagtg acccagtcag
     1261 tccagttttc agacaaccag tgcgtcacct ttttgaaggc gctttaaagc acgttttaat
     1321 ccaggtcggc ctgtccttgt tccgcttaat ttatcttcaa atatttgttc atatcctgca
     1381 caaacaagag cgtttcgttg caggtctgta ttctggtcat ttgttgatac ccttacatag
     1441 ccaatcagca cgctgaatct cccgtccaaa agcacaaatc atgccatgca ggccagaaac
     1501 cgccattatc taaaacctcg gtttacgaga actcggcaca agcggggaga aaataccgtt
     1561 actcagtaca gcgaatacct ggactaatcg acaaacattc agcggtggcc tttctggtga
     1621 actatccggc aatgcttcta cagctgcaaa attaaaaact gccaggaaaa taagcaatgt
     1681 ggcttttgat ggttcctccg atatcacatt aaaagcaagt catgttggtg cgtttgcctt
     1741 agggaaaaca ggaagcaccg ttgcgaatga taaagcagtt ggatggaact ggagtagcgg
     1801 agcctataac gcaactatta gtggtgcatc aacgttaatt attcattttt atatgggaga
     1861 aggaagttgt cctgcagctc agtttcggat taattataaa aatggcggta ttttttatcg
     1921 ttcagcccgt gatggttatg gttttgaagc cgactggtcc gaattttaca ccaccagaaa
     1981 accttcagca ggagatgttg gtgcactgcc gttatctggt ggtcaactga atggtgcact
     2041 gggtatcgga acatccagtg ctcttggcgg taattcgatt gtattgggtg ataatgacac
     2101 gggctttaaa caaaaaggcg atggtaatct ggatgtttat gctaataacg tccatgttat
     2161 gcgctttgtc tccggaagca ttcaaagtaa taaaaccata aatattacgg gacgtgttaa
     2221 tccctcggat tacggtaact ttgattcccg ctatgtgaga gatgtcagac ttggcacacg
     2281 agttgtttag accatgaaga aaggcgtgat gtatgaaaaa tcaggccatg taatcacggg
     2341 gcttggtatt atcggtgaag tcgatggtga tgatccggca gtattcagac caatacaaaa
     2401 gttaattaac ggaacatggt ataacgtatc gcaggtataa tcatgcagca tttaaaaaac
     2461 attgtcgcag gtaatccaaa aaccgttgaa caataccagc taacaaagaa ttttgatgtt
     2521 atctggttat ggtccgaaga tggaaaaaat tggcatgagg aattaaaaaa ctttcaggag
     2581 gatacaataa aactggctta cacagttgag ggaataatta ttgctataga caaggatgta
     2641 tcagcaatta atccagaagg tttaagtgtc gttgagttgc ctgatattac agcaaatcgc
     2701 cgggctgata tttcgggcaa ctggatgttc aaagatggtg cggtgataaa gcgaacttat
     2761 accgaggaag agcaaaggca acaagcggaa aatgaaaagc agagcctgct gcagctcgtc
     2821 agggataaaa cccagctgtg ggactctcag ctacagctgg gcatcatttc cgatgagaat
     2881 aaacaaaaat taaccgagtg gatgctctat gcgcagaagg ttgaatccac agacacctcc
     2941 agcctgccag taacgtttcc cgaacaacca gaatgagaga aggcccgcta tcgggcctta
     3001 atttttattc aggcttttgt ggccattcag gatttgccgt atccacacgg ctgaccagaa
     3061 cactgtagcg ttcccatgct tccagtcgtg tgcgttcctc gtctgttgcc atattcagcc
     3121 tgacagcgcg ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt
     3181 gtgattcggc ctgttgttgc agttcctgtt ctgtatattc cctctttact actttaccat
     3241 ccagaaacat ccattttccg ttattgtctg cgtgacgatt tgccgtaata tcaggaactt
     3301 caactatgct tagcccttca ggattaatag ttgaaacgtc tttactgaca gcaacgatga
     3361 tgttgttctc atcgtaagca atttttatgg tgtctggctg aaaattcttt tgttcctcat
     3421 accagttttt gccatcttcg caaaagagcc ataccacgcc cgctttttta gttagctggt
     3481 attgttccgg tgttttcgga ttcccggcct taatattttt caggtgcatc atcttaaaca
     3541 ctccccacgt tgtaccatat tcccccaatc aatttctgaa ttggccttct ggcaacagtg
     3601 tcgacaatat catccgcatt gctgttaatt gcggctgtta aaacataacc tgacgcatcg
     3661 ccaaagccgt agtcgcgcca tacgttttta tattcaatgc tgccaagacg aatatcccga
     3721 acgacatgat tatgcagcca tgtgcttaac cagttgtttt cccacactga accataaata
     3781 tcgccactag acgcaacttg cgcaccactt cccagcgcaa atcccccatc tgttttaaaa
     3841 atgaaatcac caccaccatc tgaaccgtta ttaatatgaa caccatcacc accgttatct
     3901 ttccacaaaa acatacgaat tcttccgtct tcgttggcga atgtaatgtg gtggcggtaa
     3961 ttacttttta atctaaaatt gctgccatta gattcaatat ctttataaag atgtaatcct
     4021 aactcatcaa tatgtccgat gcgcgaacca tttgaataga agctaataat accatctcca
     4081 tcttgcctaa atccggtgtc gttatctccc agaacaatgg agttttcacc cagtgcgtta
     4141 tcagtagctc caatagctag acctccttta attttggccc cgtggctgac agatatagca
     4201 cctgttctca gatttatagc gaatggcctt aatgggccga tattaccgtt ttcgccttgt
     4261 ccttcagccg tcggaatgaa atgaagaaaa tcttctgaac gacggaaaat tagaccgaag
     4321 gtatcattga aaattcgcag cgcattcacc gtgccaattt tcagttctcc ggtcatttta
     4381 tcgccggaac gctgaacggc gttaccagcc ttgtttaccg tttcctgtaa accgaggtat
     4441 tcgataacgg cagcaacggt cgatttcgca agaatatccc gcccgacttt tgtcagggtt
     4501 gccaggctgg cgacatcatt ccccgtaaaa tacggaaact tgcctgccgc agtagtaagt
     4561 ccggccagcg ccgtcagggt ggcatctttc ggttgcttac ccgcaagcgc gttagtcatg
     4621 gtggtcgcaa aattcgggtc gttgcccagc gccgcagcca gctcgttcag cgtgttcagt
     4681 gcatcaggtg acgaatctac aagggcggca atcgcagcca taacgaaagc cgtgcttgcg
     4741 atttgggtat tattcgttcc ctgtcgcgca gttggtgttg ttggcgttcc ggtcagtgca
     4801 ggactattta agggcgcttt cttgttcgtt tcacccatta ccgccttaac cgcttttggc
     4861 gttgccgcca gtgactcgga agtgctgttg gtcgcactgc tgagctgtac tatccccttt
     4921 ttcgtcgtgc tcgcatcctc cagcgccacg gcggatgcaa tatcctctgc ccgttttgct
     4981 gctgtctcgg cgcgcgttgc cgcggattca gcagcaactt tgctctgaga tgcagccgtc
     5041 gcactgcctg ccgcctctga tgctttcgtt gttgctgtcg tggcactacc tttcgctgct
     5101 gacgcttgtc tggtcgcctc atcttttgaa gcagacgcag atgatgccga tgacgccgct
     5161 gaactggctg acgatgcggc tgctgcctta gaggaagcag cattgtctgc tgaagtcttt
     5221 gcatttgttt cagaggtttt tgctgcagaa gcagacctcg ctgctgcagt ggcttgctca
     5281 gtggctttgc cagccttcgt tgtggctgtt gaagcggatg atgcggcgct ttctgccgat
     5341 tttccggcgg cggtggcact ggctgaggcc tgcccggcac ttgttgacgc ggcactggca
     5401 gataatgcag ccgctgtttt tgaacctgcc gcagctgagg cgctctgtcc cgctgctgtt
     5461 tcagaagact tagcgttcgt ctcggacgtt tttgccgcct tcgcggaatt tcctgccgcc
     5521 gttgccgagg aagctgcact actggcactt gatgatgcat tcgtttctga agatttcgct
     5581 gcctcttttg aggccgccgc accccgtgcc gaggtggcag cttctgacgc cttcgtggtc
     5641 gctgtggatg cagaagtggc tgccgatttt tgtgatgctg cggcattcgt ttctgacgtt
     5701 ttcgcggcac tggcgctggt agctgccgcg ctttttgatg actctgcagc agcacttttt
     5761 gctgcttcac gggcctttgt cgatgccgtt cctgcgctgg aagacgctga ctgagccgac
     5821 gacgcggcct gtccggctga cgtgctggcg gcacgtgctg aggctgcagc atcggttgca
     5881 tgagttgccg cctcgctggc tgattttttc gcggctgccg tattctgtgc aaccgcggag
     5941 gcgttacgtg acacctcttc caccatctgc tcaaagcggc gcagtgcctc cggtcggaca
     6001 tcatcctccg tcatggcacc gagaaaatca ttcagcgtac ctggtctgga accttcatag
     6061 acggtaatgg tcccggcatg tgaaggcgga aaaccttcaa ccagcagggt gacgctgtac
     6121 tgaccatgct caacatccat gctgtaacgt ccggcttcat ccggattttc agaggccacc
     6181 gtgttcacca ccatcgtgct gctggttcgt ctggccttca gcacaatggt gcagttctgt
     6241 actggttttc ctgtgccatc tttaagcacg ccagaaattt ttactgtcat acttttccac
     6301 caataaaaaa agcccgcagc agtgacgcca cgggcttcag gacagtgtaa ctttacgttt
     6361 cctcaaacgc agttcacccc ataaggtgga tgaacctgcg tatcataaca atatttacag
     6421 aagataaatc ggcgtctgtt gtcagatacg gtatccgata ccaacaataa atgcatccgt
     6481 tcgccagtca ccactaccgg aaccttcata agcaaggtca atggtcacgg attcggtcgg
     6541 gttaaactgc acgccagccc cccacgccag agacgtgttg ctgtggcgac cgtcatcact
     6601 tccggtcagc acatcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga
     6661 gaaagtcgac acacggctgt aagccacacc c
//
LOCUS       DAZPLM010000216         6668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPLM010000216 DAZPLM010000000
VERSION     DAZPLM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.216"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /locus_tag="ACHVC7_003675"
     CDS             complement(<1..262)
                     /locus_tag="ACHVC7_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2117618.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINIS"
     gene            complement(863..1111)
                     /locus_tag="ACHVC7_003676"
     CDS             complement(863..1111)
                     /locus_tag="ACHVC7_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR2117619.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1374..1469)
                     /locus_tag="ACHVC7_003677"
                     /pseudo
     CDS             complement(1374..1469)
                     /locus_tag="ACHVC7_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1466..2032)
                     /locus_tag="ACHVC7_003678"
     CDS             complement(1466..2032)
                     /locus_tag="ACHVC7_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR2117620.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2342..4114
                     /gene="aspS"
                     /locus_tag="ACHVC7_003679"
     CDS             2342..4114
                     /gene="aspS"
                     /locus_tag="ACHVC7_003679"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR2117621.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4232..4684
                     /gene="nudB"
                     /locus_tag="ACHVC7_003680"
     CDS             4232..4684
                     /gene="nudB"
                     /locus_tag="ACHVC7_003680"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR2117622.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4713..5453
                     /locus_tag="ACHVC7_003681"
     CDS             4713..5453
                     /locus_tag="ACHVC7_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2117623.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5488..6009
                     /gene="ruvC"
                     /locus_tag="ACHVC7_003682"
     CDS             5488..6009
                     /gene="ruvC"
                     /locus_tag="ACHVC7_003682"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR2117624.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6011..6613)
                     /locus_tag="ACHVC7_003683"
     CDS             complement(6011..6613)
                     /locus_tag="ACHVC7_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR2117625.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1677 a         1534 c         1754 g         1703 t
ORIGIN      
        1 tacttatatt aattgattta attcccgggg ggatttcagg taatgttgtc aggcctaatt
       61 cagacaagtc caggttcgtc tcttggtttt gtagacatga tactagtcgc tgaaaagcga
      121 tgtctcgttg ttcttcttgt atgcggttat ttttccattc agtccaatgg gttagataat
      181 tttcatatgc acggctagtg tcgattgaat aagtggagaa cgaatttgaa attaatctgt
      241 gattgttatt tgtcgggagc ataaatatca gggcgtattc tttatgttga gaggtgttgg
      301 attctttttt atctgctttg caatgttatt ggttcccttc agcagtagac aggaacttct
      361 ggaggaggcg gatatatagt ttgtgtaagc atattaactc catatgttat atattgaaga
      421 acttctgctt tacactatcc taaactggat cggtttaatg taaaaaaacg atactttttg
      481 ggggaggggg caatttttcc ttaaggtatg aaaaaaggag ctgaagctat ataatagcta
      541 ttaatgccac tggtatcagt gaatcatgta tataaaaaac tgtgttaagg aggctgtcca
      601 ttagcggttt tttattcttc tggaaccgcg gcatctttgc gtatcttttt gtcttctcac
      661 cgtctggtcg gtgttttgct gagactgcta atttcctgtc tttgctagtg ctgtactggt
      721 actgcccaat catgattggc gggggcgggc ggagttgaga ccgcagacac gtcgtatgca
      781 agaacgtgct gcggctggct ggtgaacttc cgatagtgcg agtattgaat gatttccagc
      841 cgttactgat tttacgtgct aattagtgaa caaaccactc gtcagcagac tcccaggtat
      901 ctttcagagt ttcctgaaca aaagttttag ctgaatcttt atcggcggtg cgcgtaacag
      961 aaaggccatc gttgctggtg gcttttacga tcacctctac atcgtcataa cgtttactga
     1021 tgcgtcgggt taattcttcc tttaacgcat ccacagcacc ggttggcatt ttagtcattt
     1081 tctctttggc tatgcagatt tcaatacgca taaaaatccc tctacactgt gtttttatac
     1141 agtattattt ttaactgtat ggataaacag tgtcaagagt gagggaaagt gattttttgg
     1201 ggcatgcatg gggcaaaaat atttgttgtg gggcattttg gggcatgatt gggacacttt
     1261 gttacatatg aactttgccg attttcatat ggcatggcaa tccaacgctt tgattattct
     1321 aaagaaaaca catgctcttg ggcgttcttt agtgattttt aaaaatcccg tatcacgcag
     1381 ttaaagtggc gggcatactc ttcaaggctg gtgatcccca accgcaccca tttaggatgc
     1441 gaccattgcg gtagaccgat gtaaatcata acgcgtgaag gatctcttcc acgctacgca
     1501 cacgggcgat gcgcgggtag atatgattaa tgctgttatt gtgttgctcg gcgctggcgg
     1561 cactacaggc gtcttcggca atcaccagat taaagccaag ttcccaggca ttgcgggcgg
     1621 tggattcaac accgatattg gtcgagatcc cacataacac tattgtatcg atacctcggc
     1681 ggcgcagttg caactccaga tccgtaccgt aaaacgcacc ccactgacgt ttgatgattt
     1741 cgatatcact gtcagttgca cctaatgcag ctggatgttg ccaccagttt tcgggcaaca
     1801 ctttagcggg ggagggggca tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc
     1861 agccaactcg caccagaaac acgggctgac cgctggcgcg aaatttcgcc gccagcttcc
     1921 cggcgcgatt aactacctca tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt
     1981 cttgtaaatc gatcaccacc aacgcagtgg ttttagcatt aagttcaagc atgctgactc
     2041 ctgaaaaaac aaaatcgtgc ctcacacctt aatgcgatta gccgatggcg gaacgacaaa
     2101 ttttgttaat ttttatgagt tcgggcaaca cctttatagc aaactggcgt agagtctgat
     2161 ttcaggcccg atataaagtg gtaacgaggc gttacagcgc gggccaggct cttacggtgc
     2221 ggcagaattt ccagtataat agccgccttt tttcattcag ttgtgacata cagctaacgc
     2281 tgcgacttgg tcacctgcgc agcagggggc atccgccggc agctaagtta agggatatct
     2341 catgcgtaca gaatattgtg gacagctccg tttgtcccac gtggggcagc aggtgactct
     2401 gtgtggttgg gtcaaccgtc gtcgtgatct tggtagcctg atcttcatcg atatgcgcga
     2461 ccgcgaaggt atcgtgcagg tatttttcga tccggatcgt gcggacgcgt taaagctggc
     2521 ctctgaactg cgtaatgagt tctgcattca ggttacgggc accgtacgtg cgcgtgacga
     2581 aaaaaatatt aaccgcgata tggcgaccgg cgaaattgaa gtgctggcgt cctcgctgac
     2641 tatcatcaac cgcgcagatg ttctgccgct tgactctaac cacgtcaaca ccgaagaagc
     2701 gcgtctgaaa taccgctacc tcgacctgcg tcgcccggaa atggcgcagc gcctgaaaac
     2761 ccgcgctaaa atcaccagcc tggtgcgccg ttttatggat gaccacggct tcctcgacat
     2821 cgaaactccg atgctgacca aagccacgcc ggaaggcgcg cgtgactact tggtgccttc
     2881 tcgtgtgcac aaaggtaaat tctacgcact gccgcaatcc ccacagttgt tcaaacagct
     2941 gctgatgatg tccggttttg accgttacta tcagatcgtt aaatgcttcc gtgacgaaga
     3001 cctgcgcgct gatcgtcagc ctgaatttac tcagatcgat gtggaaactt ctttcatgac
     3061 cgcgccgcaa gtgcgtgaag tgatggaagc gctggtgcgt catttgtggc tggaagtgaa
     3121 gggcgtggat ctgggcgatt tcccggtaat gacctttgcg gaagcagaac gccgttatgg
     3181 ttctgataaa ccggatctgc gtaacccgat ggaactgacc gacgttgccg atctgctgaa
     3241 atctgttgag tttgcagtat ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc
     3301 tctacgtgtt ccgggtggcg catcgctgac ccgtaagcag atcgacgaat acggtaactt
     3361 cgttaaaatc tacggcgcga aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg
     3421 tctggaaggt atcaacagcc cggtagcgaa gttccttaat gcagaaatca tcgaagccat
     3481 cctggatcgt actgccgcgc aagatggcga tatgattttc ttcggtgccg acaacaagaa
     3541 aattgttgcc gacgcgatgg gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac
     3601 cgacgaaagc aaatgggcac cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg
     3661 tgaaggcggc ctgacggcaa tgcaccatcc gttcacctca ccgaaagaca tgactgctgc
     3721 agaactgaaa gctgcaccgg aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg
     3781 ttacgaagtg ggcggtggtt cagtacgtat ccataatggt gatatgcagc agacggtgtt
     3841 tggtattctg ggtatcaacg aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc
     3901 cctgaaatac ggtactccgc cgcacgcagg tctggcattc ggtcttgacc gtctgaccat
     3961 gctgctgact ggcaccgaca atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc
     4021 agcgtgtctg atgactgaag caccgagctt tgctaacccg actgtactgg ctgagctgag
     4081 cattcaggtt gtgaagaagg ctgagaataa ctgatatgac tcaaatacac gaaatcattc
     4141 gcgttgcatc aaggcggcaa ctgagtgaac tcccatgagc atagataact atgtgaatgg
     4201 gatgagcgaa ggcagtcaac gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt
     4261 ttcgatctta gtggtcatct atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg
     4321 tgacgatccc gatttctggc agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc
     4381 gcaagctgcc atgcgcgaag taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact
     4441 gaccttaatt gactgtcagc gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg
     4501 ctatgcgccg ggcgtgacgc gtaatacgga atcatggttc tgtcttgcgc tcccgcacga
     4561 acgccaggta gtcttcactg aacatctggc ttacaagtgg cttgatgcgc ctgctgccgc
     4621 ggcgctcact aagtcctgga gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc
     4681 ctgaacaggc agggctatct ggagatattt ttatggcagg tcatagtaaa tgggccaaca
     4741 ccagacatcg taaagctgcg caggatgcta agcgcggtaa aatcttcact aaaatcattc
     4801 gtgagctggt aaccgcggct aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc
     4861 gtgcggcggt ggataaagca ctgtctaaca acatgacccg tgacacactg aaccgcgcaa
     4921 ttgcacgtgg tgtgggcggt gatgatgatg caaacatgga aaccatcatc tacgaaggtt
     4981 acggtcctgg cggcacggca atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg
     5041 ttgctgaagt gcgtcatgca tttagcaaat gtggcggtaa cctcggtact gatggttccg
     5101 ttgcctatct gttcagcaaa aaaggcgtga tctccttcga gaaaggcgat gaagacacca
     5161 ttatggaagc agcactggaa gcaggtgctg aagacgttgt gacctatgat gacggcgcga
     5221 ttgacgtcta cactgcatgg gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag
     5281 gtctgaaagc agacagcgcg gaagtttcca tgatcccgtc caccaaagct gatatggatg
     5341 cagaaaccgc accgaaactg atgcgtctga tcgatatgct ggaagattgc gacgacgtgc
     5401 aggaagtcta ccataacggt gaaatctctg atgaggttgc agcaactctc tgatggggcc
     5461 tgctaaacag caaaacggag acgcgtgatg gctattattc tcggcattga cccgggttcg
     5521 cgcgtgatcg gctatggtgt catccgccag gtaggcaggc aactgtccta cctgggtagc
     5581 ggatgcatac gcaccaaagt ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc
     5641 gtgacggaaa tcatcaccca gttccagcca gattatttcg ccattgaaca agtctttatg
     5701 gccaaaaatg ccgactcggc tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg
     5761 gcggtgaatc aggaattgcc ggtatttgaa tacgcggcac gtcaggtaaa gcaaacggtg
     5821 gtaggtattg gtagtgccga aaaaagccag gtgcagcata tggtccgcac cttgctgaaa
     5881 ctgcccgcta atccacaggc ggatgccgcc gatgcgttag ctatcgcgat tacccattgt
     5941 cacgtcagcc agaatgcaat gcagatgagc gaatcgcggc tgaacctggc gagagggcga
     6001 ctgcgttaag ttataccgcc tcggtcagtt caggctgagg cgtttcaact ccctccgcgt
     6061 gaatcaacac cagacccgga tgatgccaca aactggctgg cgtgacggtt ttacgctccc
     6121 acggaataga acctaagaac cagaatttcc agaaagtgag ttttgcattc ggattgctat
     6181 ttaacagttc tccaaacgtt tctatttcac ccaccggaat gcatagcgct tctttataaa
     6241 tatcaaaaac aaattttgaa cagaactggc gcgaagattc gtatttaaaa ccggtgtggt
     6301 aaagtttgcg tagccgggag ggaacctgtt caacaattcg ttgtttttgt tgttctgtca
     6361 gcccagcgtc taatcgcttt atagcatagc gttgattaga cgaacgttta ataaaacgag
     6421 atagcgtggt gatggttgaa aggggaaccc ggctttctgc aaccaggaag tcttcaccgt
     6481 tatgaccgat aatgatcccg acgtgattac tccagcaatt tgatgcagct gatatttgac
     6541 caaataaggc agcacctata catgtaaaga cgatatctcc aatttcatat tcagcagggt
     6601 aattaatatt cacgttgtca ttccattgaa atagatacga atatacgtaa tagcataaaa
     6661 aatttatt
//
LOCUS       DAZPLM010000217         6607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPLM010000217 DAZPLM010000000
VERSION     DAZPLM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.217"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..554
                     /gene="ykgH"
                     /locus_tag="ACHVC7_003684"
     CDS             <1..554
                     /gene="ykgH"
                     /locus_tag="ACHVC7_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR2117626.1"
                     /translation="LYVNKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVI
                     QRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLS
                     IVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(797..1492)
                     /locus_tag="ACHVC7_003685"
     CDS             complement(797..1492)
                     /locus_tag="ACHVC7_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR2117627.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1485..2912)
                     /locus_tag="ACHVC7_003686"
     CDS             complement(1485..2912)
                     /locus_tag="ACHVC7_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR2117628.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2923..3642)
                     /locus_tag="ACHVC7_003687"
     CDS             complement(2923..3642)
                     /locus_tag="ACHVC7_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR2117629.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHVC7_003688"
     CDS             complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHVC7_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR2117630.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5250..6575
                     /gene="rclA"
                     /locus_tag="ACHVC7_003689"
     CDS             5250..6575
                     /gene="rclA"
                     /locus_tag="ACHVC7_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR2117631.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1798 a         1631 c         1442 g         1736 t
ORIGIN      
        1 ttctgtatgt caataaagat aatataaaag tgagttacag cctaaaaata aaccagacaa
       61 caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag accacaatga
      121 ctgaagatgt tatacagcga attactacat tttttcacac cagcccagat gtcaagaaca
      181 gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa
      241 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggc
      301 aagttctcga tgagatacaa ataccttcag caattaacag tgagctttat acaaaaatga
      361 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
      421 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
      481 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
      541 actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
      601 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac tgtaaatatc
      661 ctggcattac taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt
      721 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc
      781 agatctcata tctctctcag caatcctcaa taatcagata caccgctttc accggaccat
      841 gaaccccgac gactttgata agctcaatat ccgccgttga gctgggacca ctgatgatgt
      901 taatacatga cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
      961 gcgctacacg cggcaggatt gtgcttttac gcaggataaa aagagaagat tccgggagca
     1021 ggctcaatga acgcccgcgc tcgacggcgg aaaaaagaac cacgcctccc gattcggtta
     1081 aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag atattctcgg
     1141 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc
     1201 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     1261 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     1321 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     1381 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     1441 ggcgacccag tgcctgagca acgttattca aaaattcgct ccgattatcc attctttttc
     1501 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     1561 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     1621 ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga
     1681 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt
     1741 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     1801 acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     1861 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     1921 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     1981 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     2041 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg
     2101 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     2161 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     2221 agcgtggtac acattcgcgc attaccttca ttggtcacta ggcataccga acctgtctct
     2281 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt
     2341 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     2401 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     2461 gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc aatcacctga
     2521 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     2581 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct
     2641 tctttggttt ttgcaaacca gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     2701 tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc
     2761 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     2821 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     2881 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     2941 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     3001 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac
     3061 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     3121 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     3181 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     3241 atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     3301 aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcggg
     3361 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     3421 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     3481 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     3541 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     3601 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     3661 tcatgcccat ttatgcccga tggttcccct ccttccgggg gatcaatcat caataaatat
     3721 ggcgttcttt tttaattggc tagcaacata ccacccctca tttgtgtgac aaatgataat
     3781 ctttcatcca aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt
     3841 tatggggaaa tcgacgttca tttttggcat aagaaatcca tttaaaaagg ctacatccct
     3901 tatacctatt cagagctact tattcagggg ttattgagat atacactgat ggtgtacaaa
     3961 aaaatcaccg acaataatca cgtatcaagt aatagtcggg gttttattta attgaagcaa
     4021 tttagtttgc atttttattt aaaaactgag ctagagtcca tcagaaaaaa atatataaag
     4081 cccaaaccac cgttggaaat agaaatagtt gaaaggctca caactcccgg aatgcggatt
     4141 atctttgccc tgatttctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga
     4201 tattctcccg gagtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg
     4261 gcatagccta ctgactcagc gatcaccaca acagggagca tttcccggga aaacatctgg
     4321 gccgctattt gtagacgcaa ctttgttaat acagccagcg gcgtggttcc ggaaacatca
     4381 cggaaaagct gggcaaaact tgcccgagac atgtgggcga tgctggccag cgattcgacg
     4441 gtccaggcgt gtcctggcat ttccagcatt tgctgtatta ccgcaccaag acgtggatgc
     4501 agaagcaaac tgagaatgtt tttctctgta ttaacctgtg caatccactc acgcaccgcg
     4561 agggtaaaga atgtagcgca gatctggcta cacagtgcat ccactcccgg catcgccgat
     4621 ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc agttatattc cacgctgtgg
     4681 ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta aaaaataacg cgccgaatgt
     4741 tgcaaccgaa gagtgccgca gacaatacag gtcgattcat tatcgacatg actcaggcga
     4801 tgagctgaat tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctctccc
     4861 gtcggcattt ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aaccgataat
     4921 tccccggcac catgttgaag ctgccagtca cttcctaaca cgcaattctt atcgatcgtt
     4981 ccttgtggag cgttaagcat caacaaacgg ctaagggcat ccatatcaga ctcctgagtg
     5041 gtttttgatt acaaacacca acaatcttag taaataacta atctaatacc atcaaatggt
     5101 taatcatgat ttttgttttg tgattctgat taagaaaaag aaaataccag acgataagac
     5161 aaaatatcaa gcgttgtgaa gaaatggtat ttgctctttt gcgtctatag tcatgatgtc
     5221 aaatgaacgt gtttcgacag gaaatcatca tgaataaata tcaggcagtt attattggtt
     5281 ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa agcaggttgg cgagtggctc
     5341 tcatcgaaca atcaaatgca atgtatggcg ggacctgtat taatatcggc tgcatcccaa
     5401 ccaaaacatt ggttcatgac gcacagcagc acacagattt tgtccgtgcc atacagcgta
     5461 aaaatgaagt ggttaatttt ttacgtaata agaattttca taatcttgcg gatatgccca
     5521 atatcgacgt gatcgacggc caggcggagt ttatcaataa tcatagcctg cgtgttcatc
     5581 ggcctgaggg aaatctggaa attcatggcg agaaaatttt tattaatacc ggtgcacaaa
     5641 ccgtggttcc gccaattcct ggaattacca ccacgcctgg tgtatatgac agcaccggat
     5701 tacttaatct aaaagaattg cctgggcatt taggtatttt gggcggcgga tatattggcg
     5761 ttgagttcgc ctctatgttc gctaattttg gcagcaaggt aaccatttta gaagcagctt
     5821 cgctgttttt gcctcgggaa gatcgggata ttgctgataa tatcgcgacg attttacgcg
     5881 atcagggcgt cgatattatc ctcaatgccc atgtggagcg aatcagtcac catgaaaatc
     5941 aagtgcaagt gcatagcgag cacgcccaac tggcggtgga tgcactgtta atagcttccg
     6001 gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc cggtatcgca gtaaacgagc
     6061 gcggggcaat tgtcgttgac aagcaattac ataccaccgc agacaatatt tgggcgatgg
     6121 gagatgttac cggcgggctg caatttactt atatttcact ggatgattac cgcattgtgc
     6181 gtgatgagtt gctgggagaa ggcagacgta gtactgatga tcggaaaaat gtgccttatt
     6241 ccgtattcat gacaccgcca ctgtccaggg ttggtatgac agaagaacag gccagagaga
     6301 gtggtgctga tattcaggtg gtgacattgc ctgtagctgc aattccgcgt gccagagtga
     6361 tgaatgatac tcgtggggta ttaaaagcga ttgttgataa taaaacccaa cgtatattag
     6421 gggcatcact gctgtgtgtt gactcccacg agatgatcaa tatagtgaaa atggtgatgg
     6481 atgccgggct gccttatagc atattacgcg atcagatatt tactcatccg tcgatgagcg
     6541 aatcactcaa tgatctattt tcattagtca aataaactca aaatcagacg ccagaacaaa
     6601 tattctg
//
LOCUS       DAZPLM010000218         6553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPLM010000218 DAZPLM010000000
VERSION     DAZPLM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.218"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHVC7_003690"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHVC7_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR2117632.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHVC7_003691"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHVC7_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR2117633.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHVC7_003692"
     CDS             2408..3157
                     /locus_tag="ACHVC7_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR2117634.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHVC7_003693"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHVC7_003693"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR2117635.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHVC7_003694"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHVC7_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR2117636.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHVC7_003695"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHVC7_003695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR2117637.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHVC7_003696"
     CDS             6034..6507
                     /locus_tag="ACHVC7_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR2117638.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1655 c         1659 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatgggac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAZPLM010000219         6499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPLM010000219 DAZPLM010000000
VERSION     DAZPLM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.219"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHVC7_003697"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHVC7_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHVC7_003698"
     CDS             complement(1751..2509)
                     /locus_tag="ACHVC7_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR2117639.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHVC7_003699"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHVC7_003699"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR2117640.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHVC7_003700"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHVC7_003700"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR2117641.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHVC7_003701"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHVC7_003701"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR2117642.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHVC7_003702"
     CDS             complement(5474..6079)
                     /locus_tag="ACHVC7_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117643.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="ACHVC7_003703"
     CDS             complement(6082..>6499)
                     /locus_tag="ACHVC7_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117644.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAZPLM010000220         6476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPLM010000220 DAZPLM010000000
VERSION     DAZPLM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.220"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            141..575
                     /gene="slyA"
                     /locus_tag="ACHVC7_003704"
     CDS             141..575
                     /gene="slyA"
                     /locus_tag="ACHVC7_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR2117645.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(622..1089)
                     /gene="slyB"
                     /locus_tag="ACHVC7_003705"
     CDS             complement(622..1089)
                     /gene="slyB"
                     /locus_tag="ACHVC7_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR2117646.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1363..2472
                     /gene="anmK"
                     /locus_tag="ACHVC7_003706"
     CDS             1363..2472
                     /gene="anmK"
                     /locus_tag="ACHVC7_003706"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR2117647.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2570..2899
                     /gene="mliC"
                     /locus_tag="ACHVC7_003707"
     CDS             2570..2899
                     /gene="mliC"
                     /locus_tag="ACHVC7_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR2117648.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2958..3614
                     /gene="pdxH"
                     /locus_tag="ACHVC7_003708"
     CDS             2958..3614
                     /gene="pdxH"
                     /locus_tag="ACHVC7_003708"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR2117649.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3743..5017
                     /gene="tyrS"
                     /locus_tag="ACHVC7_003709"
     CDS             3743..5017
                     /gene="tyrS"
                     /locus_tag="ACHVC7_003709"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR2117650.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5076..5939
                     /gene="pdxY"
                     /locus_tag="ACHVC7_003710"
     CDS             5076..5939
                     /gene="pdxY"
                     /locus_tag="ACHVC7_003710"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR2117651.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5983..>6476)
                     /gene="gstA"
                     /locus_tag="ACHVC7_003711"
     CDS             complement(5983..>6476)
                     /gene="gstA"
                     /locus_tag="ACHVC7_003711"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR2117652.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
BASE COUNT         1642 a         1601 c         1671 g         1562 t
ORIGIN      
        1 gaaaaattta cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg cagcaataca
       61 tttgttttag caatacaatt gctgcacact attctaaaag ccgcataata tcttagcaag
      121 ctaattataa ggagatgaaa ttggaatcgc cactaggttc tgatctggca cggttggtgc
      181 gcatatggcg tgctctgata gaccatcgcc tgaaaccgct ggagttaaca caaacccatt
      241 gggttacgtt acacaatatc catcagttac ctccagacca gtcgcaaatt caactggcaa
      301 aagcgattgg catcgagcag ccatcactgg tccgtactct ggaccaactg gaagaaaaag
      361 ggttaatttc gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg
      421 aaaaggcaga gccactgatc agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa
      481 tattacatgg catctccgca gaggaactgg agcaactgat tacgctcatc gcaaaacttg
      541 agcataatat cattgagtta caggccaaag ggtgaaatga agggggcgtg tggtcagtaa
      601 cctgaccaca cgcaaactta tttagcgcgg agaaacggtc acctgactgc cattgctggc
      661 cagtacgaca cgttggcccg gagagaaacg agtgttgcct tgtttctgta ccaccatgat
      721 ggtattacca tcgtctttac gaatttccag ctcgacaccc tgcgttttgt tcattgcact
      781 ctgtacgccc tgaccagcta cgccacctgc aacagcgcct gctgcagtag ccagagaacg
      841 cccggttccg ccaccaacag tattccccag gaaaccacca agaacagcac cgccaattgc
      901 accgataacg ttggaatcat caccgccctg aatctgtacc ggacgtacgt taacgatggt
      961 gccatagctg acattctgta cttgtttcgc ttcagaagcg gtataaacat cccctgacag
     1021 ggtgtcgtta ttaacacaac cgacaagaga cagacctacc attgaaacaa ccaatacgcg
     1081 tttaatcatt gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc
     1141 aaagtatatg gcatatgtga atccatggtt acatgttcta ccaaatatga gcaaatcata
     1201 atgaaagtag gatcaaccaa ttataaacaa aacattcatg atgattattg atttgaagat
     1261 aatgttttac taccaacggt aaaaatcggt cattccgcat ggtatccacg aaaagtttat
     1321 gtttgagtgg tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt
     1381 attggcgtta tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc gacaatagat
     1441 gaacaccggg tcgcacagct ggcaagcttg agctggccga tcccggtgtc tctgaaacag
     1501 gctgtactgg atatttgcca gggccagcag cttacacttt cgcagtttgg acagcttgat
     1561 actcaactcg ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg
     1621 caggcgagag atatagttgc gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg
     1681 ggcgtggcac cacacacttt acagattggc gataacaatc aaattgtggc acgcaccgga
     1741 attacggttg tcggtgattt tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg
     1801 ctggtacctg cgttccatca tgccttgctg gctcacccaa ccgagcgacg aatggtgctc
     1861 aatattggcg gcatcgccaa tctgtcactg ctcattcctg ggcagccggt tggcggctac
     1921 gataccggtc ctggtaacat gctgatggat gcctggatct ggcgtcaggc cggtaaacct
     1981 tacgataaag atgccgagtg ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat
     2041 atgctcagcg acccatattt ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt
     2101 aactacggct ggctggagcg ccatttacgc cattttccgg gtgttgatcc ccgtgatgtt
     2161 caggccacac tggcagaact caccgccgtg accatttctg aacaagtttt gttgagcggt
     2221 ggctgcgaac gattgatggt atgtggtgga ggtagtcgta acccgctact catggcgcgt
     2281 ctggcggcat tactgccagg cacagaagtc accaccaccg atgccgttgg cattagtggc
     2341 gatgacatgg aagcattggc attcgcctgg cttgcctggc ggacgctggc gggattacca
     2401 ggaaatctgc cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttccct
     2461 gctaacccgt gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttga
     2521 aacgttaggg agggcgtatt gccctccaga ccaggaaagt cttcgggaaa tgtctatgaa
     2581 aaaactgtta ctgatctgtt tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact
     2641 ggttgagcac atgcaaaccg ctacgctgga ataccagtgt gatgaaaaac cgttgacggt
     2701 caaactgaat aatccgcgcc aggaagtcag ttttgtttac gataatcaac tactgcatct
     2761 caaacagggc atttcagcgt ctggtgcacg ttacactgac ggaatctatg ttttctggtc
     2821 gaaaggcgat gaagcgactg tctataaacg cgaccgcatc gtattgaata actgtcagtt
     2881 acaaaatcca cagcgttgag atttttccag gggcggcgca caatagcgtc acccactgac
     2941 aatccgtaaa gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc
     3001 gtgaatacac caaaggcggg ttacgccgtc gcgatcttcc cgccgatcca ttaacccttt
     3061 ttgaacgttg gctctctcag gcgtgtgaag ccaaactggc agaccctacc gcgatggtgg
     3121 tcgctaccgt ggatgaacat gctcagcctt atcaacgcat cgttttactc aaacattacg
     3181 acgaaaaagg catggtgttt tacaccaacc tcggcagccg taaagcacat caaattgaaa
     3241 ataatccgcg cgttagcctg ctgttcccgt ggcataccct tgagcgccag gtgatggtga
     3301 tcggtaaagc ggaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc
     3361 gtgatagcca gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta
     3421 tccttgaaag taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc
     3481 cgagcttttg gggcggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg
     3541 agcatcgcct gcatgaccgc tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc
     3601 gtcttgcacc ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg
     3661 cactttattc tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt
     3721 cgttatatac atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg
     3781 ggggctggta gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc
     3841 gatcgcgctc tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt
     3901 tccattgtta tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg
     3961 cggcgcgacg ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac
     4021 cgaagaaact gttcaggagt gggtggacaa aatccgcaag caggttgccc cgttcctcga
     4081 tttcgactgt ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat
     4141 gaatgtgctg accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa
     4201 caaagaagcg gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt
     4261 ttcctacaac ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt
     4321 gctgcaaatt ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg
     4381 tcgtctgcat cagaatcagg tgtttggcct gaccgttccg ctgatcacca aagcagacgg
     4441 caccaaattt ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc
     4501 gtacaaattc taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa
     4561 gttcttcacc tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag
     4621 cggtaaagca ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg
     4681 tgaagaaggt ttgcaggcgg caaaacgtat taccgaatgc ctgttcagcg gttccttgag
     4741 tgcgctgagt gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat
     4801 ggaaaagggc gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg
     4861 tcaggcgcgt aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga
     4921 tcctgaatac ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg
     4981 cggcaaaaag aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt
     5041 gagaaatcac tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc
     5101 agtctcacgt tgtttatggt catgcgggta acagtgcggc agagtttccg atgcgccgcc
     5161 tgggcgcgaa cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca
     5221 aatggactgg ctgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg
     5281 ctattgataa attacacacc tgtgatgccg tgttaagtgg ctatctggga tcggcggagc
     5341 agggtgaaca tatcctcggt atcgtccgtc aggtgaaagc agcgaatccg caggcgaaat
     5401 atttttgcga tccggtaatg gggcatccgg aaaaaggctg tatcgttgca ccgggtgtcg
     5461 cagagtttca tgtgcggcac ggtttgcctg ccagcgatat cattgcgccg aatctggttg
     5521 agctggaaat actctgtgag catgcggtaa ataacgtcga agaagcggtt ctggcagcgc
     5581 gcgaactcat tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct
     5641 acagccgtga ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg catatcagcc
     5701 gtccactggt ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt
     5761 tactgctggt gaaactgctt cagggggcaa cgctgcagga ggtcctggaa catgtgaccg
     5821 ctgcagtcta cgaaatcatg gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg
     5881 tggctgctca ggatcgtatt gccaaaccag aacattactt cagcgcaaca aagctctgaa
     5941 atatcacatt tcggcccaca acgacctgtg ggccgatggc tattacttta agccttccgc
     6001 tgacagcgcg tcttgtactt ccggacgttc agccatacgt tgcataaatg ccgcaatgtg
     6061 ctctaaccct tccagattca gtttcaccgc gtatgcccag cgcagaaccg taaacagata
     6121 ggcatcagca attgtaaatc tttgcccgca gatccagtgc tcatccttca gtgcctcgtt
     6181 cacatattgc agcttcttat ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt
     6241 atctgggcga aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca
     6301 ttcgatggtt ttatagcggg aaatactgtt taccggtgcc agcaactggc ggtcggggac
     6361 gctgtcggca agatactgca taatcgctac gccttccgtc agcaaagtac cgtcatccag
     6421 cagcaatgct ggcacctgcc ccttagggtt aacggcaaag taatcgtcac cgtttt
//
LOCUS       DAZPLM010000221         6403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPLM010000221 DAZPLM010000000
VERSION     DAZPLM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.221"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..367
                     /gene="ydjO"
                     /locus_tag="ACHVC7_003712"
                     /pseudo
     CDS             1..367
                     /gene="ydjO"
                     /locus_tag="ACHVC7_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(371..1762)
                     /gene="tcyP"
                     /locus_tag="ACHVC7_003713"
     CDS             complement(371..1762)
                     /gene="tcyP"
                     /locus_tag="ACHVC7_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR2117653.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1895..2485)
                     /locus_tag="ACHVC7_003714"
     CDS             complement(1895..2485)
                     /locus_tag="ACHVC7_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2117654.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2648..3316)
                     /gene="hxpB"
                     /locus_tag="ACHVC7_003715"
     CDS             complement(2648..3316)
                     /gene="hxpB"
                     /locus_tag="ACHVC7_003715"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR2117655.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3463..3999
                     /locus_tag="ACHVC7_003716"
     CDS             3463..3999
                     /locus_tag="ACHVC7_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR2117656.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4040..4900)
                     /locus_tag="ACHVC7_003717"
     CDS             complement(4040..4900)
                     /locus_tag="ACHVC7_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR2117657.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5006..5296)
                     /gene="ghoS"
                     /locus_tag="ACHVC7_003718"
     CDS             complement(5006..5296)
                     /gene="ghoS"
                     /locus_tag="ACHVC7_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR2117658.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5397..6326)
                     /gene="pfkB"
                     /locus_tag="ACHVC7_003719"
     CDS             complement(5397..6326)
                     /gene="pfkB"
                     /locus_tag="ACHVC7_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR2117659.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1662 a         1598 c         1564 g         1579 t
ORIGIN      
        1 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
       61 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
      121 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa
      181 acgtctcggg tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg
      241 tattgataag ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat
      301 cgccgcgatg gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc
      361 agcataagaa ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat
      421 cggtttgctt cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta
      481 gcgccgtacg gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta
      541 ctggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac
      601 cggcaacgcc tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt
      661 ccagcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac
      721 cggcgcagcc gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt
      781 caggaacgcc cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg
      841 aacggctggt aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac
      901 tcacgccatt aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt
      961 aggacgcgac aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca
     1021 ctttggtcat tagagccaga acgccgtaag gggtcaactg catgacaagg cgaaccagtt
     1081 tcatcaccca gctttgtagg gtatcgatag cggctaagac gcgttcacct ttcggcgcat
     1141 catccttcag cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt accacgctga
     1201 taattgacgt cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca
     1261 agaccagctg cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt
     1321 tcagacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt
     1381 tggtgaccag cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg
     1441 tcagaaaact gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa
     1501 tagaggcgaa gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa
     1561 cgatgttaaa ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat
     1621 gcagggcaag gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac
     1681 tccactgttt atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt
     1741 tcgcaattaa tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta
     1801 gcagtcatag tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg
     1861 aattgcttat tccagaaagg tgatttttgc ctttttagta ttcaacctga tgcttaataa
     1921 gccgatgaaa ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt
     1981 tctcgggtaa tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac
     2041 gttccagttg gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg
     2101 gccagagcag gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac
     2161 ccagcaccat tccttgtagc gcatcagccg gaatgaacca gccttccgga accttaaggt
     2221 aaaaggttgc cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa
     2281 aagcgcgggc gatcggtttt gatatccatt ttaatcgctg cccaagaaac gactttgggt
     2341 gatcgatgtc cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac
     2401 cctgtgccag cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa
     2461 agagaaggtg accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat
     2521 aggattttta tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga
     2581 ttaatccgtc agttgaatgc aaattcagat aaagaaccag gggcagcgtg atcactgccc
     2641 ctggtcatta accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt
     2701 ttgctaatac aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca
     2761 tgcgggctgc tttagaagcg atcatgccat ttaccgagtc ttccagcgcc acgcaggtca
     2821 gtgggtcaac gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt
     2881 aaggcagttt ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca
     2941 acactttttc cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt
     3001 ctttgcataa cgcaacagct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca
     3061 gtgaaatggc acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg
     3121 gttgccgggc gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca
     3181 gctcgttacg acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat
     3241 cccataaagg ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct
     3301 gacgcggggt tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt
     3361 gcttatgatt aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca
     3421 ggtaaactaa gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct
     3481 ggacgcattg ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc
     3541 tccacattga tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt
     3601 aatgcggtca tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct
     3661 tttttgaatc tgttctggta taactccccg acccctaatt ttaatggtgg cgtgaacgtt
     3721 atgttctggg tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc
     3781 cggatgagtc gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa
     3841 aaagccaaag gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg
     3901 caccatacta ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct
     3961 gcaggttatg tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg
     4021 ccattatcct caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa
     4081 ccaaatgctg accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg
     4141 gttgacgttc aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt
     4201 gtggcggctg ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt
     4261 agcaggcagg gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata
     4321 aatcgccgtg caacagagaa ggctgcagtt gatgcgaggc cagacgctgc tgaatatgct
     4381 cgacgatggc gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc
     4441 agccaatccg ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg
     4501 gagttgtgga gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat
     4561 gtaaacgcgc aatttgctga ccaagaataa atgcgctatg cgcatccagt ggacgaggtg
     4621 ggagataatc catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag
     4681 gcacggtgac ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac
     4741 cgggaagcag ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat
     4801 gccatgcggc atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac
     4861 ctaactgctc gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc
     4921 tgccagatca taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac
     4981 agactgatta ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaagtcg
     5041 tgatggtgat ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc
     5101 taatttcttc ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga
     5161 tatttccctc atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca
     5221 actcgttaat ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa
     5281 gatcgccact ggccatagtt cacccctttg ttatcacatg acttttgagc aaagtcggag
     5341 aaacgcgttg ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag
     5401 cgggaaaggt aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga
     5461 ttgagtgtgg ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca
     5521 ttttctgcca gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc
     5581 tggcttttca ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct
     5641 ccttgtggac caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc
     5701 gcggctttgc ggacatcgtc cggctgggtg agttcgcgat tcaccagcgc actgagttct
     5761 ttttggttag gcttaaccaa ctcgatgtta ccaattgcca gtgctgcact taacgcttcg
     5821 ccagaactgt cgacgatgca gcggatccct tgtttttgcg aagcggaaat cagttgggtt
     5881 aatttttcca gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat
     5941 tcaatttcca gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct
     6001 ggcataacaa aacgatactg ctcaccgctt gcttccacat gtacatgtaa attctgccgg
     6061 gtccagtctt tggcttctac agtagcgacg gggacatttt catccgccaa cagtgaaacc
     6121 aggtgttcgc cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg
     6181 gcaatggcgc gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag
     6241 cgcagttttc cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca
     6301 agtgtcaacg tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac
     6361 cgaatcagcg gaaagtgaag aaattaacaa tatgatttat tgg
//
LOCUS       DAZPLM010000222         6360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPLM010000222 DAZPLM010000000
VERSION     DAZPLM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.222"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="ACHVC7_003720"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="ACHVC7_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR2117660.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHVC7_003721"
     CDS             complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHVC7_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR2117661.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="ACHVC7_003722"
     CDS             2736..3797
                     /locus_tag="ACHVC7_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR2117662.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHVC7_003723"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHVC7_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR2117663.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="ACHVC7_003724"
     CDS             5678..6295
                     /locus_tag="ACHVC7_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR2117664.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1715 a         1600 c         1519 g         1526 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctgggatat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgccag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcatacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 gggggctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatgttat ttattattca gtaactttat ccacaatgat gggcgtaatt
//
LOCUS       DAZPLM010000223         6316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPLM010000223 DAZPLM010000000
VERSION     DAZPLM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.223"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHVC7_003725"
     CDS             <1..411
                     /locus_tag="ACHVC7_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2117665.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            457..807
                     /gene="traA"
                     /locus_tag="ACHVC7_003726"
                     /pseudo
     CDS             457..807
                     /gene="traA"
                     /locus_tag="ACHVC7_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            822..1133
                     /gene="traL"
                     /locus_tag="ACHVC7_003727"
     CDS             822..1133
                     /gene="traL"
                     /locus_tag="ACHVC7_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR2117666.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1155..1337
                     /locus_tag="ACHVC7_003728"
                     /pseudo
     CDS             1155..1337
                     /locus_tag="ACHVC7_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            1339..2589
                     /locus_tag="ACHVC7_003729"
                     /pseudo
     CDS             1339..2589
                     /locus_tag="ACHVC7_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2586..2972
                     /gene="trbI"
                     /locus_tag="ACHVC7_003730"
     CDS             2586..2972
                     /gene="trbI"
                     /locus_tag="ACHVC7_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR2117667.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2969..3280
                     /locus_tag="ACHVC7_003731"
                     /pseudo
     CDS             2969..3280
                     /locus_tag="ACHVC7_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            3275..4273
                     /locus_tag="ACHVC7_003732"
                     /pseudo
     CDS             3275..4273
                     /locus_tag="ACHVC7_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4293..5040
                     /gene="traX"
                     /locus_tag="ACHVC7_003733"
                     /pseudo
     CDS             4293..5040
                     /gene="traX"
                     /locus_tag="ACHVC7_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            5095..5655
                     /locus_tag="ACHVC7_003734"
     CDS             5095..5655
                     /locus_tag="ACHVC7_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR2117668.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5785..5997
                     /locus_tag="ACHVC7_003735"
     CDS             5785..5997
                     /locus_tag="ACHVC7_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR2117669.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1501 a         1530 c         1927 g         1358 t
ORIGIN      
        1 gtggggtggc gggtctcatc gtccatggag acgacattcg tgctggatgc actggagcag
       61 gcgttatggg cccgtcgacc gtccggcacg gtccatcaca gtgataaagg ttctcagtat
      121 gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc aacaggaagt
      181 acaggcgact cgtatgacaa cgcgatggcg gagagcatca atggtcttta caaagcggag
      241 gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg aactggccac actcacgtgg
      301 gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc tccggcagaa
      361 gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga
      421 taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg tacttctgcg
      481 cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggtt
      541 cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc tatggctgct
      601 ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac cttcggtaag
      661 gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc agtcatgtac
      721 atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt atttattgca
      781 gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga gacgagaata
      841 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
      901 cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca acatcgaaat
      961 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
     1021 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
     1081 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
     1141 accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
     1201 cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc attcaggggg
     1261 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaatg
     1321 tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat tttcttccgg
     1381 ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc cggtaaaacc
     1441 gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc
     1501 gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata tctggacggt
     1561 gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc agcggagcgt
     1621 gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga agtgcatgaa
     1681 ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag ggcacgtatt
     1741 gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg
     1801 actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac tgccaacggc
     1861 acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg
     1921 gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc
     1981 ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa gaaactgaac
     2041 gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt
     2101 gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat cacacagaac
     2161 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
     2221 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
     2281 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
     2341 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
     2401 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
     2461 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
     2521 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
     2581 gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc agccactggt
     2641 ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc agttacggca
     2701 ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact gtggatgcat
     2761 tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc
     2821 gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac cacgcggtca
     2881 ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa atccagcagg
     2941 atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg ccctgctgat
     3001 atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa
     3061 ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc agtccggtga
     3121 gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc tgcggccccc
     3181 tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg acccgtccgt
     3241 cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg agtcagccta
     3301 cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc gtcagggctg
     3361 gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt ttgagccgaa
     3421 accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg agctgcggga
     3481 cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag acagtcctgc
     3541 acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc cggcgtttga
     3601 ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg acggaaacgg
     3661 gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc agttcgtggc
     3721 cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg atggcgtccg
     3781 gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg aaggccgtcc
     3841 gtggaatccc ggtgccatca ccgatggccg cgtgtggggg gatatcccgg acaacagcgt
     3901 ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac agcggcaggc
     3961 tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc gtaaaatggc
     4021 agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg aaattgccgg
     4081 gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg agagtgtgct
     4141 gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa atctgctgca
     4201 ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag agaaaacccc
     4261 gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg aacacgacac
     4321 gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg gtctggtcac
     4381 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccacatca
     4441 atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga gcctttccgc
     4501 tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg caaccggcca
     4561 ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg gcaggctttc
     4621 cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
     4681 gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc ctgttggggg
     4741 cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt cagccaccga
     4801 ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt
     4861 attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct tgtgatgacg
     4921 gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg
     4981 gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga
     5041 cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca cagcatgaca
     5101 gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac gcctgtccgc
     5161 agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag
     5221 ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca ggccagacag
     5281 gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac cctgaaaccc
     5341 tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg tatccgggac
     5401 gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa actgcgcagg
     5461 gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc
     5521 cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga agcgtatgcg
     5581 gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact tcaggccgtg
     5641 cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata aggcttaaaa
     5701 ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc acaataatac
     5761 tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg gaacggccag
     5821 gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag tggacgctgg
     5881 atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac agactggcag
     5941 cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag ggtgtaacga
     6001 aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat gagatttgct
     6061 ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagttttttc
     6121 cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt
     6181 gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat atggagaata
     6241 acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc caacgtggga
     6301 tatttaacgc gggtta
//
LOCUS       DAZPLM010000224         6272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPLM010000224 DAZPLM010000000
VERSION     DAZPLM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.224"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHVC7_003736"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHVC7_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2117670.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHVC7_003737"
     CDS             2219..2767
                     /locus_tag="ACHVC7_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR2117671.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHVC7_003738"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHVC7_003738"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR2117672.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHVC7_003739"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHVC7_003739"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR2117673.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHVC7_003740"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHVC7_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR2117674.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1556 a         1552 c         1597 g         1567 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta cc
//
LOCUS       DAZPLM010000225         6242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPLM010000225 DAZPLM010000000
VERSION     DAZPLM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.225"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            45..1505
                     /locus_tag="ACHVC7_003741"
     CDS             45..1505
                     /locus_tag="ACHVC7_003741"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR2117675.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHVC7_003742"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHVC7_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR2117676.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHVC7_003743"
                     /gene_synonym="ydfH"
     CDS             complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHVC7_003743"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR2117677.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHVC7_003744"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHVC7_003744"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR2117678.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="ACHVC7_003745"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="ACHVC7_003745"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR2117679.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="ACHVC7_003746"
     CDS             complement(5669..6187)
                     /locus_tag="ACHVC7_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR2117680.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1553 a         1489 c         1604 g         1596 t
ORIGIN      
        1 taacaaaaca tatagcttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcactgt tttttgtcca cgctattata tgtaaaggtg atttgctttt tctgtatgct
     6241 cg
//
LOCUS       DAZPLM010000226         6221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPLM010000226 DAZPLM010000000
VERSION     DAZPLM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.226"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(129..503)
                     /locus_tag="ACHVC7_003747"
     CDS             complement(129..503)
                     /locus_tag="ACHVC7_003747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117681.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(826..2760)
                     /locus_tag="ACHVC7_003748"
     CDS             complement(826..2760)
                     /locus_tag="ACHVC7_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2117682.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            2840..4774
                     /gene="yeaG"
                     /locus_tag="ACHVC7_003749"
     CDS             2840..4774
                     /gene="yeaG"
                     /locus_tag="ACHVC7_003749"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR2117683.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4887..6170
                     /locus_tag="ACHVC7_003750"
     CDS             4887..6170
                     /locus_tag="ACHVC7_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR2117684.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         1726 a         1466 c         1313 g         1716 t
ORIGIN      
        1 tgtaaagtac aaagcctact atctcgtaaa gtaatttagt aaaccattcc attttgaagt
       61 acccccattc acattcaact ttatagtatt tagtgaggga gtacttaaaa cgtaacaatt
      121 tggatgtgtt agctaataag ttttagtata ggtttcgaat aaatgacaag tccagtaact
      181 gaacattttg cttcttttat ctcttcaatt agttccattg atattggatg tttgaaagct
      241 tgagtacact cctgacagcc tgcctccgtt agcggaaact catctgttat aacttgaatt
      301 tcagaagaat acggcaaaaa aatgtactga actttaattt tgttggaaaa ttcgtacttg
      361 cataagctct catcttgaga atcattccgc tttgaaaaaa gccttaattg attctcttca
      421 ttcaatgaga tcccaatatc ataattctca gggaaaaatt gacggaattt ttcttttttt
      481 gctttatttt ctttcttacg cattttggca atcctctctt tagaattcat ctcatggttt
      541 catcgctcct attcgttagt gaatgacgct atggtactgg tttaacgttg aaaagtcaaa
      601 aaagttaatt ttacccttga ctattctaac acacgctgat ataaatgatt tgctgtattt
      661 tgcacggaaa tcttaaaccc atcgggtgcg tagttatatc caaaattcat aatccctctt
      721 ttattgctct atctacaatg ccgttagatt tcgacatcgt agatatattg ttcaaatatt
      781 aactcgcata attcattcta caagaccata aattctcatt agcttttaat ccatccaatc
      841 aatcatatct tcattaacaa tgatctcaaa tttttcacgc ttgagaagat taagatacaa
      901 tccaacatca tcaagtattt tggtacggct aatggcaagc gaccacatat cagtaccaga
      961 tgtaacggag gcaatgaaaa cggttttgcc ctcttcattg acagtgtaga cacgctcgcc
     1021 gcttgtttca gagattacga tctcatgtag tacttcaccc ccgccccgct tgttatcaat
     1081 cacaagcgta tgggatagga catcattgaa gtattccaac tgtacaaata tacgcttttc
     1141 aaaaacccag tacttaacag atttcagtac taatcttact tctttccgta actttgcacg
     1201 taactcgaca ttcaatggat caagtacttt attaatatcg aaatcaaata cagggatttc
     1261 tttgatgttc tttgaattta atatctcaga ttctatttct tcaattctat cctgtacctc
     1321 agttaaaccc gtcattaatg gtaacgaggc tttcttacct gctgccttac gttcattaat
     1381 tttaacctga taggtttctg cctctttctg taaagtactt aactcttctc gcaatgatgc
     1441 aagcaatgaa tctgaatccg acttaccttc catcatcaat ttattgaagt ctacattttt
     1501 gacaagctgt agaatgttct tttcaattct catgtactgg atagatttag catcgcagat
     1561 cttcttttct tccctgttac gacaaataac atacttagtt cgtacatgat tatagtgaat
     1621 tgcaatattg cccccacagt gaccgcattt cagcatcttc gggaagaggt tagtaatgta
     1681 atcatcctga gcaatggtaa tgtccttacg gatacgatta gctataccat tccgcgataa
     1741 cttctcatta gcccgttcaa aggtgaggtt atcgataatc ttcatggtaa ctactttctt
     1801 atcacgtagt accctcagta cattgacggt actccaccct ttcggcaagc gttttacaat
     1861 cgcccctgaa ccaaggcctg tctcataaag tttaaaaatc tctcggacaa cttctggttc
     1921 aagtaataga tcctctgctg gcttcttctt ctccaacata acgcgatcca tcattctatt
     1981 cgctgtcagg aacaactctt cgtcgatgat tttcgggtaa acgtttggta ctacctcacc
     2041 gttgtaacgt tcatgctcgc ccaacaaacg gcgatcacgt atcagcctgg acacagtgac
     2101 catctgccac tttctaccga tacgtttaac aatctcgcca gtactcaacc cttccctata
     2161 ccacatgaaa catttaacaa tcagttctgc ctgttccggt ataacttggt acttgttatc
     2221 tacatttctc aaccaattag gcatacggtt ggagattact tccccgttct gtactgcacg
     2281 tgtgattttg ttatcccatg ccaattttgc acgtaccgat ttcatcaatg actcattgtg
     2341 tgaacggtgc tgtatcagtt ccatgtgaat ttttgacata ggatcatcaa cccgtaacac
     2401 aaggtttgta gagatatcgt gaatctcaat cccactattc acgatgtact ggatagtact
     2461 ttcggcccag cttgatcgac gcgatagacg atccagcgat aaaaccacaa gtgcgtcacc
     2521 tacgccgtac ttgcggttgc ccacatcttg caaaaatatc ccaagctgag actctggaga
     2581 gatattggca tttttgaatg cggaaagccc cgcatcagta atgaaaatcc tttcgtcagt
     2641 gaacttatca gcatttttat ctaagtatgc agtcaatgat gatcgttggt cgtccaaacc
     2701 tttacccata tcaacttgct gaatgcttga tactcgctca taacaaatag gacggagcat
     2761 attttttatc ccgttgaaga attacgctaa aagtgctgat tcacaaatgt tcccagagta
     2821 tacaaaagga cggcatacca tgaatatatt cgatcactat cgccagcgat atgaagctgc
     2881 caaggacgaa gagttcacac tgcaggagtt tcttaccact tgtcggcaag atcgcagtgc
     2941 ttatgccaac gcggctgagc ggctattgat ggctatcggt gagcctgtca tggtcgatac
     3001 agcccaggaa cccagacttt ctcgactctt ttctaaccgg gtcattgcac gttatccggc
     3061 atttgaagag ttttacggca tggaagacgc gattgaacag attgtctctt atctgaaaca
     3121 cgcggctcag gggctggaag agaagaaaca aatcctgtat ctgctggggc ctgtgggtgg
     3181 gggtaaatca tcgcttgctg agcgactgaa atcattaatg cagctcgtac cgatttatgt
     3241 attgagcgcc aacggtgagc gtagcccggt caacgatcat ccgttctgtc ttttcaatcc
     3301 gcaggaagat gcgcagattc tggaaaaaga gtatggcatt cctcgccgct atctcggcac
     3361 catcatgtcg ccgtgggcgg caaaacgcct gcatgaattt ggtggcgata tcactaagtt
     3421 ccgggtagtg aaggtctggc cgtcaattct gcaacaaatt gctatcgcca aaacagaacc
     3481 cggtgatgag aacaaccagg acatctccgc gctggtaggg aaagtcgata ttcgtaaact
     3541 cgaacactac gcgcagaatg acccggacgc ctacggctat tccggtgcgc tgtgccgcgc
     3601 caatcagggg atcatggaat ttgtcgagat gtttaaagca ccgattaaag tgctgcatcc
     3661 gctgttaacc gctactcagg aaggtaacta caacgggacg gaaggtatct ccgccctgcc
     3721 gttcaacggg attattctcg cccactcgaa cgaatccgaa tgggtcactt tccgtaataa
     3781 caaaaacaac gaagccttcc tcgaccgtgt ttacatcgtg aaggtgccgt attgcttacg
     3841 catttccgaa gagatcaaaa tctacgagaa attgcttaat cacagtgaat tgactcacgc
     3901 cccatgcgcc cctggcacgc tggaaacact gtcacgtttt tccattcttt cgcgcctgaa
     3961 agagccagaa aactccagca tttattcaaa gatgcgggtt tatgatggcg aaagtctgaa
     4021 agacaccgat cccaaagcca agtcgtatca ggaatatcgt gactacgccg gtgtcgatga
     4081 agggatgaaa ggtctgtcga cgcgttttgc gtttaagatc ctctcccgcg tgttcaactt
     4141 cgatcatgta gaagtggcgg caaacccggt ccatctgttc tacgtcctag aacagcagat
     4201 cgagcgcgag cagttcccgc aagagcaggc agaacgctat ctggagttcc tgaaaggtta
     4261 tctgatcccg aaatatgccg agtttatcgg caaagagatc cagacggcct accttgaatc
     4321 ctattccgaa tatgggcaaa acattttcga ccgttatgtt acctacgcgg atttctggat
     4381 tcaggatcag gagtatcgcg atccggatac cgggcagctg tttgaccgcg agtctcttaa
     4441 cgccgagctg gagaaaatcg agaaaccggc ggggatcagt aatccaaaag atttccgcaa
     4501 cgagattgtt aacttcgtac tgcgcgccag agcgaataac agcggacgca atccgaactg
     4561 gaccagctat gaaaaactgc gcacggttat cgagaagaaa atgttctcca ataccgagga
     4621 gctgttgccg gttatctcgt ttaacgccaa aacgtcaacc gacgagcaga agaaacacga
     4681 cgactttgtc gaccgtatga tggaaaaagg ctacacccgt aaacaggtgc gtttactgtg
     4741 cgaatggtat ttgcgcgtac gtaaatcgtc ttaacaaccc tggcccggta cacatgctac
     4801 cgggcctata acgacagcga accgtgggcc agagaaatgg caacacaggc gtagcataca
     4861 gttggcaaat gtagtacggg gggcatatga ccgggtttat tgaccggcgt ctgaacggca
     4921 aaaacaaaag catggtgaat cgccagcgtt ttttacgccg ttataaagcg caaattaaac
     4981 agtcgatctc cgaggccatt aataagcgtt cggtgactga cgtcgacagc ggcgaatccg
     5041 tatccattcc cacggaagat attagcgaac cgatgtttca tcaggggcgt ggcggcctgc
     5101 gccaccgcgt acatccgggc aatgaccatt tcgtccagaa cgaccgaatt gaacgtcccc
     5161 agggtggcgg cggaggttcc ggcagtggtc agggccaggc cagccaggat ggtgaaggtc
     5221 aggatgaatt tgtctttcag atttcgaaag atgagtatct tgatctgctc tttgaagatt
     5281 tggccttacc gaatctgaaa caaaaccaac aacgccagct gaccgaatat aaaacgcatc
     5341 gggcgggtta taccgctaac ggcgttccag ccaatatcag cgttgtgcgt tccttgcaga
     5401 actcactggc gcgacgcaca gccatgacgg cgggcaagcg acgggaactt catgcgctgg
     5461 aagagaattt ggccatcatc agcaacagtg aacctgcgca actgctggaa gaggaacgtc
     5521 tgcgcaaaga aattgcagaa ttacgtgcca aaattgaacg cgtccctttt attgacacct
     5581 tcgatttacg ttacaagaac tacgagaagc agcccgatcc ctccagccag gcagtgatgt
     5641 tttgcctgat ggacgtttcc ggttcaatgg atcaatccac taaagatatg gctaagcgtt
     5701 tttatattct gctgtatctg ttcctcagca gaacgtataa gaacgtggaa gtcgtataca
     5761 tccgccatca tacccaggcg aaagaagtcg atgaacatga gtttttctac tcgcaggaaa
     5821 caggcggcac cattgtttcc agcgccctga aactgatgga tgaggtagtg aaagagcgtt
     5881 ataacccggc acagtggaat atttacgctg cacaagcatc ggacggcgat aactgggccg
     5941 atgactctcc gctttgccat gaaatcctgg cgaaaaaatt attacctgtt gttcgttatt
     6001 acagctatat cgaaattacc cgtcgtgcac atcagacatt gtggcgagaa tatgagcatc
     6061 tgcaatctac tttcgacaac tttgcgatgc agcacatccg cgaccaggat gatatttatc
     6121 cggtgttccg tgaactgttt cataaacaaa atgcaacagc taaagactaa aactatcagc
     6181 caggtcatta tcgcctggct gatttttagc ttactgtaaa t
//
LOCUS       DAZPLM010000227         6215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPLM010000227 DAZPLM010000000
VERSION     DAZPLM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.227"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHVC7_003751"
     CDS             complement(10..165)
                     /locus_tag="ACHVC7_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2117685.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHVC7_003752"
     CDS             complement(368..775)
                     /locus_tag="ACHVC7_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2117686.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHVC7_003753"
     CDS             852..1079
                     /locus_tag="ACHVC7_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR2117687.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHVC7_003754"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHVC7_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHVC7_003755"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHVC7_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..3114
                     /locus_tag="ACHVC7_003756"
     CDS             2368..3114
                     /locus_tag="ACHVC7_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2117688.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3129..3551
                     /locus_tag="ACHVC7_003757"
     CDS             3129..3551
                     /locus_tag="ACHVC7_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2117689.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3609..3965
                     /locus_tag="ACHVC7_003758"
     CDS             3609..3965
                     /locus_tag="ACHVC7_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117690.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4134..4799
                     /locus_tag="ACHVC7_003759"
     CDS             4134..4799
                     /locus_tag="ACHVC7_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR2117691.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5027..5182
                     /gene="hokD"
                     /locus_tag="ACHVC7_003760"
     CDS             5027..5182
                     /gene="hokD"
                     /locus_tag="ACHVC7_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR2117692.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5432..5719
                     /locus_tag="ACHVC7_003761"
     CDS             5432..5719
                     /locus_tag="ACHVC7_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117693.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5758..>6215
                     /locus_tag="ACHVC7_003762"
     CDS             5758..>6215
                     /locus_tag="ACHVC7_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2117694.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
BASE COUNT         1644 a         1349 c         1690 g         1532 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acattgctcg gcgagcgggt gatggatcgc atggtcatga acggcgggcg ctgggtgaat
     3061 tttaactggg agagctggcg tccgaatgtt agccattcga gggttgttaa gtagtttcag
     3121 gaggatttat ggcgaaacct tttactcccg aacagcggga agaactgaag acgcgaattg
     3181 tggaactcgt gcatcaggac ggtcgggtca cgattcggca gttgtcagat gaaacaggta
     3241 tcagtcgtgc gtctgtcggt cgcttatgca tagaactggt cgcaagtggt gatgtatata
     3301 attctggcta cggcttattc ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca
     3361 aaaaactctc gagagtaaag gtgaggaaac cggttgttgt tgatccggac cttatctggt
     3421 cattacctga cggagaaata cgccgctacg acaggcgcct gaatataatc tgtcgcgagt
     3481 gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcaggagg
     3541 cgatactgtg aatgaaatta gctatcaggc ttcaattacc gctggcattc gcatcaaagg
     3601 agaggagcat ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccggtaa
     3661 aagaggatga ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt
     3721 gccatcacgc ctctgtacgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg
     3781 gtgcggttgt ggagccgttt gactggatgc tctctgtggc aaaaagagaa accagactgg
     3841 cagatgatgt aaggctattg cgccaggagg aacaggaaag gcggagaaat atagaaaagc
     3901 tgatacagat tgagcgtaac gcgaaagcgc ggatacgcag ggcgacaaaa tccagaactg
     3961 aataattaaa tttagcactg ttaaaaattt aatccttaac cggagggatt tctgcaccct
     4021 caaatcatca ggagaccacc cgaaagggcg gggagcagtc acacatctgt ttccgatagc
     4081 cccgttctaa tgctacactc tttgatattt ttatgacccc aataaacata tttatgacag
     4141 ttgctgattt caaacggccc aaattggagc tcccaaacgg ggcaaacaaa ctactactgc
     4201 actcttgctg tgctccatgt tccggtgagg tgatggaggc gcttcaggcc tcgggaatcg
     4261 actacaccat ctttttctac aacccgaaca ttcatcctca gaaagagtat ttaattcgta
     4321 aggatgagaa tattcgcttt gctgaacaac acggcgtgcc gtttattgat gctgattacg
     4381 acacagacaa ctggtttgaa cgagccaaag gaatggaatg ggagcccgaa cgagggatcc
     4441 gttgcaccat gtgttttgac atgcgttttg agcggacagc gctgtacgcc gctgaaaatg
     4501 gtttcagtgt gatcagcagt tcactgggca tttcacgctg gaaaaatatg cagcaggtta
     4561 acgactgtgg gcggcgagcc gtcgcgcatt atccgggcat ggtgtactgg gattataact
     4621 ggcgcaagca gggcggctcg tcccgtatga ttgaaatcag caagcgcgaa aaattctatc
     4681 agcaggaata ttgtggctgt gtgtattctc tgcgcgatac caatctacac cgcaaatctc
     4741 agggacgccc tcttatcaaa attggtcaac tccactacgg taaagaagag aaggagtgat
     4801 tttatggggc acctttctga ttgatttcat attggcgagg taagtagaat gactgcgggt
     4861 gcttgaggct atctgcttca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
     4921 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccaatttca tgctttgcac
     4981 atgtaggtta gcctcttaca tgccgaaagg caaggagaag caggctatga agcagcaaaa
     5041 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     5101 gaggaaagac ctctgtgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcgt
     5161 agactacgaa tccaggaagt aagagtgacc gggcggggag ctgatcccat ccccgcccac
     5221 ctctgatgtg tcaggcatcc tcaacgcacc cgcacttaac ccgcccatcg ctgtgatctc
     5281 tcagcgtttc ggcgggtttt ttgttgttta tttccggtga atttgattcg cgcaccttcg
     5341 cagatagaat cgactcactt aagtagcgcg cagggagaag agggatggac cccgaacagg
     5401 ggagagctat ttatctggaa ggattctgaa gatgaaaatc gaagaattgc gtgaaatttt
     5461 tagtgaaaat ggcctctatg ctgtgcgcgt tgagaatgga gcgattgtca gccattgccg
     5521 cattagatgt ttgcaatctc aacaaaggaa gagcggtgct gtgttatttt atttttgtaa
     5581 tggacttctg acggacggtt ttattttgcg tgaggacgaa tttgtcacat cattacgggt
     5641 tttgaaagag attggtttta aggctggttt tctgcttttg ctgaagaata aactcatcta
     5701 caatcttgag caggattgaa ctcctgctgt gtaacaccgt gccaccggag aaagccgatg
     5761 gcacatatac aactggtcaa acaaacctct tccggattac ttctcccggc gacgccggag
     5821 agttgcgatt ttctgcatca aatcaaaata ggtgagtgga tacacgcaga ctttaagcgt
     5881 gtgtgtaact acgcattcca caagcgtttt ttcaaactcc tgcaactggg tttcgattac
     5941 tggactccgg tcggtggggc gatcacgcct cgcgaacgaa aactggtgtc cggtttcgtt
     6001 gattacctgt gtgaatcagt aggccgggaa catacgccag ctctgagcga agccgcagag
     6061 caatatctga atacagttgc gacacgcaga accagtgata cggcattgct aaagtcgttt
     6121 gaggctttcc gcgagtgggt agccattcag gccggatttt acaccgagca tttttatccg
     6181 gacggtagcc gtgggtgtcg ggcgaaatct atcgc
//
LOCUS       DAZPLM010000228         6208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPLM010000228 DAZPLM010000000
VERSION     DAZPLM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.228"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHVC7_003763"
     CDS             <1..331
                     /locus_tag="ACHVC7_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR2117695.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHVC7_003764"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHVC7_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR2117696.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHVC7_003765"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHVC7_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR2117697.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHVC7_003766"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHVC7_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR2117698.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHVC7_003767"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHVC7_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR2117699.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHVC7_003768"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHVC7_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR2117700.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..>6208)
                     /gene="ompN"
                     /locus_tag="ACHVC7_003769"
     CDS             complement(5142..>6208)
                     /gene="ompN"
                     /locus_tag="ACHVC7_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR2117701.1"
                     /translation="VYNKDGNKLDLYGKVDGLHYFSDNSAKDGDQSYARLGFKGETQI
                     NDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDYGRNYGVMYDIEGW
                     TDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQGNNEGASNGQ
                     EGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHTAAGGDKADAW
                     TAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQFDFGLRPAVSF
                     LMSKGRDLHAADGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYKINLLDEDDSF
                     YAANGISTDDIVALGLVYQF"
BASE COUNT         1683 a         1474 c         1508 g         1543 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaact
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaactt
//
LOCUS       DAZPLM010000229         6128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPLM010000229 DAZPLM010000000
VERSION     DAZPLM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.229"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            87..863
                     /gene="exuR"
                     /locus_tag="ACHVC7_003770"
     CDS             87..863
                     /gene="exuR"
                     /locus_tag="ACHVC7_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR2117702.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1103..1186
                     /gene="yqiM"
                     /locus_tag="ACHVC7_003771"
     CDS             1103..1186
                     /gene="yqiM"
                     /locus_tag="ACHVC7_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR2117703.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1208..1870
                     /gene="yqjA"
                     /locus_tag="ACHVC7_003772"
     CDS             1208..1870
                     /gene="yqjA"
                     /locus_tag="ACHVC7_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR2117704.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1874..2257
                     /gene="mzrA"
                     /locus_tag="ACHVC7_003773"
     CDS             1874..2257
                     /gene="mzrA"
                     /locus_tag="ACHVC7_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR2117705.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2404..2772
                     /gene="yqjC"
                     /locus_tag="ACHVC7_003774"
     CDS             2404..2772
                     /gene="yqjC"
                     /locus_tag="ACHVC7_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR2117706.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2810..3115
                     /locus_tag="ACHVC7_003775"
     CDS             2810..3115
                     /locus_tag="ACHVC7_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR2117707.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3118..3522
                     /locus_tag="ACHVC7_003776"
     CDS             3118..3522
                     /locus_tag="ACHVC7_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR2117708.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3512..3811
                     /locus_tag="ACHVC7_003777"
     CDS             3512..3811
                     /locus_tag="ACHVC7_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR2117709.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3997..4389
                     /locus_tag="ACHVC7_003778"
     CDS             3997..4389
                     /locus_tag="ACHVC7_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR2117710.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4459..5444
                     /gene="yqjG"
                     /locus_tag="ACHVC7_003779"
                     /pseudo
     CDS             4459..5444
                     /gene="yqjG"
                     /locus_tag="ACHVC7_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5737..6102
                     /locus_tag="ACHVC7_003780"
     CDS             5737..6102
                     /locus_tag="ACHVC7_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2117711.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1539 a         1510 c         1542 g         1537 t
ORIGIN      
        1 atcaccaaat ttatatatga ccaatggcat acccatgaat aaagtggtat gataacttcg
       61 tccgcattcc actccgggag acgcatatgg aaatcactga accacgccgt ttgtatcaac
      121 aacttgccgc tgacctgaaa gagcgcattg aacagggcgt ctatctggtg ggtgataaac
      181 tgcctgccga acgctttatt gccgatgaaa agaacgtcag ccgaacggta gttcgtgaag
      241 ccatcatcat gctggaagtt gaaggctatg tggaggtgcg taaaggttca ggtattcatg
      301 tggtttccaa ccagccgcgc catcagcagg cggctgacaa taatatggaa ttcgccaatt
      361 acggtccgtt tgagttgctt caggctcgcc agctcatcga aagtaatatt gccgagttcg
      421 cggcaactca ggtaacgaaa caagacatca tgaaactgat ggccattcag gaacaggcgc
      481 gcggcgaaca atgcttccgt gattccgagt gggatttaca gttccacatt caggtagccc
      541 tggcgacgca gaactccgcc ctggcggcca tcgttgaaaa aatgtggacc cagcgtagcc
      601 ataacccgta ctggaaaaaa ctacacgaac acattgattc ccgtaccgtt gataactggt
      661 gtgatgacca cgatcaaatt ctcaaggcgt taattcgcaa agatccccat gctgctaagc
      721 tggcaatgtg gcagcatctg gaaaacacca aaatcatgtt atttaacgaa accagcgacg
      781 atttcgagtt caatgccgac cgctatctat tcgccgaaaa cccggtagtg catctcgata
      841 cggccaccag tggcagtaaa tgaaactcct gtccgacagg cgctgcgacg cgcctgttcg
      901 cttcgtcggt aagcgaaaag tataaagtgt cagcctgtgt aaatcctctc gccaccctcc
      961 cctgcattca gcaaaatcag actccacgga catgtaattt tgataacgaa caacgttgac
     1021 ctttgttaca attagattca atttgaattt atgtttttga atgctttctt atctcacgat
     1081 ttaacaggga atagttcagg ctgtgttgat gtatcaaacc cgcagaacat accaaaacag
     1141 caataacatt gcggtagtgc atcttttaaa accagcgtgg cgttaaccga ttcaccagga
     1201 ataatgaatg gaacttttga cccaattgct gcaagccctg tgggcgcagg attttgaaac
     1261 cctggccaat ccatcgatga ttggcatgtt gtattttgtc ttgtttgtaa ttttgttcct
     1321 tgaaaacggc ttgcttccag cggccttttt accgggcgac agtttactgg tattggtcgg
     1381 cgtgttgatt gcgaaaggcg cgatgggtta cccgcaaacg attctgctgc tgaccgttgc
     1441 cgccagcctc ggctgctggg tcagctatat tcaggggcga tggctgggca atacccgcac
     1501 cgtacaaaac tggctatctc atttacccgc gcattatcat caacgcgcac accatctttt
     1561 tcataaacac ggtttatcgg cgctgttaat tggtcgcttt attgcgtttg tcagaacact
     1621 gctgccgacg attgccgggt tatcagggct gaataacgcg cgctttcagt ttttcaactg
     1681 gatgagcggt ctgctgtggg tattgatcct gacaactctg ggttacatgc tcggcaaaac
     1741 gccgatattt ttaaagtacg aggaccagct gatgtcatgc ctgatgctgc tcccggtggt
     1801 gctgctggtg tttggcctgg caggttctct ggtcgtgtta tggaaaaaga aatatggaaa
     1861 tcgggggtaa gggatgcaaa tacctcgcat gtcgcttcgc cagctagcct ggtccggcac
     1921 tgttttactt ctggtcggca cgctgttgct ggcctggtcc gcggttcgcc agcaagagtc
     1981 tacgctggcg attcgtgccg ttcatcaagg cacaacgatg ccagacggtt tttcgatctg
     2041 gcatcacctt gacgctcatg gcattccttt caaaagtatc acccccaaaa acgacactct
     2101 gttaattaca tttgactcca gcgaccagag cgccgccgca aaagcggtcc tcgacagaac
     2161 attgccccat ggctacatca ttgcgcagca ggacaataac agtcaggcta tgcagtggct
     2221 gacccggtta cgggataact ctcatcgctt cggataactt cctgttatcc gaaacatatc
     2281 actcactttg gtgatttcac cgtaactgtc tatgattaat gaggcggtgg ttaacactac
     2341 cgcttcgttg tctggacaat cgttcctttg taataggtcc gaatcacaat ggaaggttca
     2401 agaatgaaat accgcatcgc tttagctgtt tctctctttg ctcttagtgc cggtagttat
     2461 gccactaccc tgtgtcagga aaaggagcaa aatatcctta aggagatcag ctatgccgaa
     2521 aaacaccaaa accagaatcg tattgacggt ctgaataaag ccctgagtga agtccgggcc
     2581 aactgttcag atagccagct gcgtgccgat catcacaaga aaatcgcaaa gcagaaagat
     2641 gaggtggcgg aacgccagca agatttagcc gaggcgaagc aaaaaggcga tgccgataag
     2701 attgccaaac gcgaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg
     2761 cgcgactact aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga
     2821 acacactacg gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt
     2881 gcttagctca tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga
     2941 gcaggcactg aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca
     3001 aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt
     3061 gggaattggc gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg
     3121 gcggacactc atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt
     3181 gtttccatca tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa
     3241 gaggaaaaag ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct
     3301 gcatttggtc ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc
     3361 ctgaatgcga tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc
     3421 tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca
     3481 aacgatcggc aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg
     3541 taaggcgcaa ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg
     3601 tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg
     3661 ctcctgggcg ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat
     3721 gctggtccgc tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac
     3781 gaccctcaag cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct
     3841 tatccctcaa attttttgaa gatttttgac agttttcctt gctaacaatc atcattcacc
     3901 acgtttatga ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa
     3961 acaatcagca gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt
     4021 gtactggtag cgcgcatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc
     4081 accgcttact ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg
     4141 ccgctggtga ttctgcttga gttcggtggt ggtctggcaa tcctgttcgg tttcctgact
     4201 cgcaccacag ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc
     4261 aactttgctg aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga
     4321 ttcctgctgc tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa
     4381 aagtggtaag cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg
     4441 ctattggagg agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac
     4501 gataccaaat ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc
     4561 actgccgatg gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt
     4621 tatcatctct atatttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa
     4681 ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac
     4741 ggctggacct ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa
     4801 tttctgtatc agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc
     4861 gtactgtggg acaaaaagaa ccacaccatc gtcagcaacg aatcaacaga aatcatccgc
     4921 atgttcaata ccgcgtttga tgcgctgggc gcgaaagcgg gtgattacta cccaccagcc
     4981 ctgcaaacga aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg
     5041 tataaagctg gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt
     5101 gaatcgctgg cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag
     5161 ctaaccgaag ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg
     5221 acccacttca agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg
     5281 cgcgatatct accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat
     5341 cattacttcc gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg
     5401 caggatctcg atgaaccgca ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg
     5461 aagtgcctct gataattagc taagtgcatg ttgttcatat cggatgcggc gtgaacgcct
     5521 tatcaaacct acaaaacatt tatattcaat atgttgcaat gaccgcgcag gccagataag
     5581 cgtagcgcat ctggcgattt tgcgcttgtc atcagtctta gcgcgccttc acagcacttt
     5641 attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc
     5701 tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtaata
     5761 aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg
     5821 gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc
     5881 gctggtggcg aaggcatcct gacgacaatc tacggtattc tggtgttttt accgtggtgg
     5941 gcagttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgctattc
     6001 ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca
     6061 ggcgaaaacc gctttggacc tgatccaaaa ctggaacctt aaaacaaatc tgctcgccgg
     6121 atgtctga
//
LOCUS       DAZPLM010000230         6122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPLM010000230 DAZPLM010000000
VERSION     DAZPLM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.230"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHVC7_003781"
     CDS             <1..358
                     /locus_tag="ACHVC7_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2117712.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHVC7_003782"
     CDS             complement(398..1069)
                     /locus_tag="ACHVC7_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR2117713.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHVC7_003783"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHVC7_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR2117714.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHVC7_003784"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHVC7_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR2117715.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHVC7_003785"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHVC7_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR2117716.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHVC7_003786"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHVC7_003786"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR2117717.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHVC7_003787"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHVC7_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR2117718.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHVC7_003788"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHVC7_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR2117719.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6122)
                     /gene="fliD"
                     /locus_tag="ACHVC7_003789"
     CDS             complement(5600..>6122)
                     /gene="fliD"
                     /locus_tag="ACHVC7_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR2117720.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1489 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 at
//
LOCUS       DAZPLM010000231         6083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPLM010000231 DAZPLM010000000
VERSION     DAZPLM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.231"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(182..1852)
                     /gene="betA"
                     /locus_tag="ACHVC7_003790"
     CDS             complement(182..1852)
                     /gene="betA"
                     /locus_tag="ACHVC7_003790"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR2117721.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1866..3338)
                     /gene="betB"
                     /locus_tag="ACHVC7_003791"
     CDS             complement(1866..3338)
                     /gene="betB"
                     /locus_tag="ACHVC7_003791"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR2117722.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3352..3939)
                     /gene="betI"
                     /locus_tag="ACHVC7_003792"
     CDS             complement(3352..3939)
                     /gene="betI"
                     /locus_tag="ACHVC7_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR2117723.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4078..6030
                     /gene="betT"
                     /locus_tag="ACHVC7_003793"
                     /pseudo
     CDS             4078..6030
                     /gene="betT"
                     /locus_tag="ACHVC7_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1226 a         1693 c         1700 g         1464 t
ORIGIN      
        1 taaggttgta ttaatagtct gtatcaggaa tgttcggggt aaatatcagc aaaaaaccag
       61 catcatgaat actggatatg aagcatgaga gttacctcag tgtttatata aggattcggt
      121 ccccctctct ggaacggtaa ctctcaatct gatcggttcc tgcgttagtt cacatcacga
      181 ctcatttttt cgctctcacc ggcatcccat ttgccacaaa atatcccgcc gtgctcctcg
      241 gcagcgcttc ctgtccacga atcatatccg ctattttctc gccaatcata attgtcgtgg
      301 cgttcaaatt cccggtgata atctgcggca taattgacgc atccaccacg cgcaggcctt
      361 ccagcccatg cacgcggcct tcgccgtcaa ccacggacat ctcgtcgtaa cccattttgc
      421 aggtaccgca cggatggaag gcggtttcgg cgtgattacg cacgaactca tcgagctgct
      481 catccgtctg acattccgtg ccggggctga tttcgcggcc acgatactga tccagcgtcg
      541 gttgatgcat gatctcccgg gtgatgcgaa ttgcgtcgcg gaactcctgc cagtcctgct
      601 cgtgtgacat gtagttaaac agaattgccg gatgctggtg cgggtcgcgg gatttaatcc
      661 gcacatgccc acggcttggc gagcgcattg agccgacgtg gcactggaaa ccgtgctcct
      721 tcaccgcatt cgagccgtta tagttaatcg ctactggcag gaaatggtac tgaatattcg
      781 gccacgcaaa ttcctctcgg ctgcgaataa atccgcctgc ttcaaagtgg ttgctggcac
      841 caacgccagt gccgccaaac agccactccg caccgatttt cggctggttc caccactgca
      901 gggcagggta gagggaaacc ggttctttgc actcatattg cagatacatc tccagatgat
      961 cctgaagatt ttcgccgacg ccgggtaatt catgcaccag cggaatatca aactccgcca
     1021 gcagttcagc gttgccgacg ccggagcgtt gcaggatctg cggtgaggca atcgcgcctg
     1081 cacataacag cacttctttg ttggccgttg cgcgggttgg gatggtgctg tcgccttcca
     1141 gccattcgac gcccaccgcg cgtttgccgt caaaaatgat gtgatcggtc atagcgtgag
     1201 tacgaatggt caggttaggg cgcgatttgg cctgatcgag ataaccgcgc gcggtactgg
     1261 cgcgacggcc ctgcggcgtg acggtgcgat ccatcggacc aaaaccttcc tgctgataac
     1321 cgttgagatc gtccgtgcgc gggtagcccg cctgcacgcc cgcttcaatc atcgcttcaa
     1381 acagcggatt gacgccgggt ttggaggtag tgacgctcac cgggccatcg ccaccgtggt
     1441 agtcgttctc gcccacatcg cgagtctcgg ccttgcggta gtagggcagg cagtcgagat
     1501 aactccagtt ctccagaccg ggttcttgcg cccagttatc gagatccagc gcattgccac
     1561 ggatgtagca catgccgttg atcagcgacg atccacccag gcctttaccg cgtccgcact
     1621 ccatgcggcg gttattcata aacggttcag gctctgtttc ataggcccag ttgtagcgtt
     1681 tgccctgtag tgggaacgcc agcgctgcgg gcatctgggt gcggaagtca aagcgatagt
     1741 ccgggccgcc cgcttcaagc agcagcacgg aggtattcgg atcttcagtc agacgggtag
     1801 cgagaacgtt gccggctgag ccggcaccaa taatgatgta gtcaaattgc aaataaacct
     1861 cctggttaga atatggactg gaatttagcc atctcaacct ggatggactt cacctgggtg
     1921 taactctgga gcgtcatcac gccgttctcg cgaccaatgc cggagtgttt gtagccgcca
     1981 acgggcatct ctgccgggga ttcgccccag gtgttgatcc agcaaatacc cgcttccagc
     2041 tgatgaatga cgcgatgcgc gcggttcagg tccgccgtca cgatgcccgc cgccaggccg
     2101 tagtcggtat cattggcgcg gcgaatcact tcgtcctcag attcataagt gagaagcgac
     2161 atcaccggac cgaagatctc ttcgcgcacg atggtcattt cgtcgcggca atcggtgaac
     2221 acggtcggtg caacccatgc gccgttatcg aagccatcgc ctttcagtac atcgccgccg
     2281 cacagtacgc gcgcgccttc ctctttgcct ttggcgatat agcgcagcac gttatcgcga
     2341 tgcgggaagc tgaccagcgg gccgaagtta gtttgcggat cgaaaacgtc gcccgcgcga
     2401 atgcgctcaa cgcgcgccag aattttctgc tcaaatgcgg ctttgcattt cgccggaacg
     2461 aagacgcggg tgccattggt acacacctga ccggagctga agaagtttgc catcatggcg
     2521 atatcggcgg cgagatcgag atccgcatca tcgaaaacga tcagcggtga tttaccgccc
     2581 agttccatgg tcacttcttt cagggaagag gccgccgagt tagccatcac ttttttgccg
     2641 ctggcgacac cgccggtaaa tgacactttg gcaatgcccg gatgctcggt cagatattgc
     2701 ccggtctccg cgcccacccc cggcaacacg ttaaatacgc cgtccggcag gcccgcttcg
     2761 ctgtaaattt cagccagctt taacgcggta agcggggtga cttcgctcgg tttgaaaatc
     2821 attgcgttgc ctgccgccag cgccggggcg gatttccaca gggcaatctg aatcgggtag
     2881 ttccatgcgc caatccccgc caccacaccc agcggttcgc ggcgggtata aacaaatgac
     2941 gtctcacgca acgggatctg gctgccttcc agcgccggga tcagcccggc gtagtactcc
     3001 agcacgtccg caccggtaac gatatcgacg gttgaggttt ccgaatatgc ttttccggta
     3061 tcgagggttt ccagttttgc gagttcgtca ttgcgttcac gcagaatatc aacggcccga
     3121 cgcagaatac gcgagcgctc catagcggtc atcgccgccc agattttttg cccctgttgg
     3181 gcactcttca cggcgcgatc gacatcctca cgcccggcgg cctgcacggt cgccagcacg
     3241 ttaccgttgg ccgggttaat ggtctcgaag gtgcgaccgc tggtggcgga ggtataacca
     3301 ccatgtatat aaagctgctg ttctgccatt cgggacatca cgtctcctcg gttaatcggt
     3361 gggtaaatgc tgagtaataa agtgacgggt cagcgagtgt gcgagaggtt tatccagcgg
     3421 tttgccgctt agtgccgcgc gcagccataa tccgtcaatc agcgcggcaa ggccgtagcc
     3481 cgcttcctgt gcctgttcgc gcggcaattc gcggcgaaac tcgctcacca gattcgacag
     3541 caagcggcgg ctgctgacct gctgtaaacg atagagcatc ggctgatgca tactgctggc
     3601 ccagaacgcc agccaggctt tcatcgccgc actgctcacc tgagtttcat cgaagtttcc
     3661 ggcaacaatc gcctgtaatc gctgctctgc actgccctgc ggaagtgcat gtaatcgatt
     3721 caaaaccgcg tcacgcagct gactggtgat atcgcgcatg gttgcttcca gcagaccatt
     3781 tttgtccctg aaatagtggc tgatgatccc cgtagaaacg cctgcacggc gggcgatctg
     3841 cgcgatcgtt gcatcgtgca tgcccacttc atttattgct tccagtgtgg cgtcgatcag
     3901 ttgtctgcgc cggatcgact gcatccccaa tttgggcatt ttcgccactc cattcatcag
     3961 cgttgtttat ctattaaagc ggttattgat tggacgttca atataaaatg tgtcgtaatt
     4021 gttacgaatt tgattttaaa tggtaacaat aacagtgggg atactggatg acagaccttt
     4081 tccctgacaa cgatcctgtt tcgcgacttc tcggccctgt ggattggccg cacgctggac
     4141 tgggtttcta aaaccttcgg ttggtactat ctactggcgg caacgctcta tattgtcttt
     4201 gtggtctgta tcgcttgttc gcgttttggt tcggtgaagc tcgggccaga acaatccaaa
     4261 ccggaattca gcctgctgag ttgggcggcg atgctgtttg ctggcgggat cggtatcgac
     4321 ctgatgttct tctccgtagc cgaaccggta acgcagtata tgcagccgcc ggaaggcgcg
     4381 ggacagacga ttgaggccgc gcgtcaggcg atggtctgga cgctgtttca ctacggctta
     4441 accggctggt cgatgtatgc gctgatgggc atggcgctcg gatactttag ctatcgttat
     4501 aatttgccgc tcaccatccg ctcggcgctg tacccgatct tcggtaaacg gattaacggg
     4561 ccgataggtc actcagtgga tattgcagcg gtgatcggca ctatcttcgg tattgccact
     4621 acgctcggta tcggtgtggt gcagcttaac tatggcttga gcgtactgtt tgatattccc
     4681 gattcgatgg cggcaaaagc ggcactgatc gccttgtcgg tgataatcgc cacgatctct
     4741 gtcacctccg gtgtcgataa gggcattcgt gtgttatcgg aacttaacgt tgcgctggcg
     4801 ctgggattga tcctgttcgt attgtttatg ggcgacacct cgttcctgct taatgcgctg
     4861 gtgctgaacg ttggcgacta tgtgaatcgc tttatgggca tgacgctcaa cagttttgcc
     4921 ttcgaccgtc cggttgagtg gatgaataac tggacgctct tcttctgggc atggtgggtg
     4981 gcatggtcgc cgtttgtcgg cttgttcctg gcgcgtatct cgcgtgggcg taccattcgc
     5041 cagttcgtgc tgggcacgtt gattattccg tttaccttca cgctgttatg gctctcggta
     5101 ttcggcaata gcgcgctgta tgaaatcatc cacggcggcg cggcatttgc cgaggaagcg
     5161 atggtccatc cggagcgcgg cttctacagc ctgctggcgc agtatccggc gtttaccttt
     5221 agcgcctccg tcgccaccat tactggcctg ctgttttatg tgacctcggc ggactccggt
     5281 gcgctggtgc tggggaattt cacctcgcag cttaaagata tcaacagcga cgcccccggc
     5341 tggctgcgcg tcttctggtc ggtggcgatt ggtctgctga cgctcggcat gctgatgacc
     5401 aacgggatat ccgcgctgca aaacaccacg gtaatcatgg ggctgccgtt cagctttgtg
     5461 attttcttcg tgatggcggg gttgtataaa tctctgaagg tagaagatta ccgccgtgaa
     5521 agtgtcaacc gcgataccgc gccgcgaccg ctggggcttc aggatcgcct gagctggaaa
     5581 aaacgtctat cgcgcctgat gaattatccg ggcacgcgtt acactaaaca gatgatggag
     5641 acggtctgtt acccggcaat ggaagaagtg gcgcaggagc tgcggttgcg cggcgcgtac
     5701 gtggagctaa aaagcctgcc gccggaagag ggacaacagt tggggcatct ggatttgttg
     5761 gtgcatatgg gcgaagagca aaactttgtc tatcagattt ggccgcagca atattcggtg
     5821 ccgggcttta cctaccgcgc acgtatcggt aaatcgacct actaccgact ggaaaccttc
     5881 ctgttagaag gcagccaggg caacgacctg atggactaca gcaaagagca ggtgatcacc
     5941 gatattcttg accagtacga gcggcacctt aactttattc atctccatcg tgaagcgccg
     6001 ggccatagcg tgatgttccc ggacgcgtga ttgtttttgc ataaaaccgc gccatgtctg
     6061 catatggcgc gggaatcatt gac
//
LOCUS       DAZPLM010000232         6025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPLM010000232 DAZPLM010000000
VERSION     DAZPLM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.233"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(127..276)
                     /locus_tag="ACHVC7_003794"
     CDS             complement(127..276)
                     /locus_tag="ACHVC7_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117724.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            847..1005
                     /locus_tag="ACHVC7_003795"
     CDS             847..1005
                     /locus_tag="ACHVC7_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2117725.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="ACHVC7_003796"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="ACHVC7_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR2117726.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="ACHVC7_003797"
     CDS             2367..2669
                     /locus_tag="ACHVC7_003797"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117727.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="ACHVC7_003798"
     CDS             complement(2845..4338)
                     /locus_tag="ACHVC7_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR2117728.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="ACHVC7_003799"
     CDS             complement(4382..4771)
                     /locus_tag="ACHVC7_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117729.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="ACHVC7_003800"
     CDS             4989..5429
                     /locus_tag="ACHVC7_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR2117730.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1428 a         1663 c         1581 g         1353 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     5881 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     5941 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt
     6001 tcatcgcaac tgcatgaatt aaaag
//
LOCUS       DAZPLM010000233         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPLM010000233 DAZPLM010000000
VERSION     DAZPLM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.234"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHVC7_003801"
     tRNA            292..368
                     /locus_tag="ACHVC7_003801"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHVC7_003802"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHVC7_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR2117731.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHVC7_003803"
     CDS             complement(1357..2247)
                     /locus_tag="ACHVC7_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR2117732.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHVC7_003804"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHVC7_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR2117733.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHVC7_003805"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHVC7_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR2117734.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPLM010000234         5925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPLM010000234 DAZPLM010000000
VERSION     DAZPLM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.235"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1508
                     /gene="tssI"
                     /locus_tag="ACHVC7_003806"
     CDS             <1..1508
                     /gene="tssI"
                     /locus_tag="ACHVC7_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR2117735.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1576..5571
                     /locus_tag="ACHVC7_003807"
                     /pseudo
     CDS             1576..5571
                     /locus_tag="ACHVC7_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1415 a         1503 c         1919 g         1088 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcgttaacc
     1021 agatagagac ggtgggcagt aaccagataa ttaaagtggg ttctgttcag gtggaaacga
     1081 ttggacttgt tcgtgcgctg acggtgggcg tggcgtatca gacgacggta ggtggcatta
     1141 tgaacacctc ggtggcactg atgcagtcct cgcagatcgg tttgcataaa tcgttgaggg
     1201 tcgggctggg ttatgacgtc aaagtcggaa ataacgttac cttcaccgtt ggtaaaacga
     1261 aaaaggatga taccgggcag accgcgattt actccgccgg tgagcatctg gagctctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggacaaat ttttctcaac ggcacaaaaa
     1381 ttcatttgca gggtaaggag caggttaatg gtgactcact gttgattaac tggaactgtg
     1441 cagcctcgaa atccccaccg aagacccctg atgaaaagca ggatacgccg gatatgagag
     1501 agtactgatc ccgtacctgt tcggtattca gtaacaaacc agcattaaaa ataaacagaa
     1561 gaaggaattt gaatcatgag cggaaaacca gcggcgcgtc agggagatat gactcagtat
     1621 ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg gcgcgcccac cggcgtggcg
     1681 tgctcggtgt gtccgggcgg gatgacttcg ggcaacccgg taaatccgct gctgggggcg
     1741 aaggtgctgc ccggcgagac ggaccttgcg ctgcccggcc cgctgccgtt cattctctcc
     1801 cgcacctaca gcagctaccg gacgaagacg cctgcaccgg tgggcgtttt cggccccggc
     1861 tggaaagcgc cttctgatat ccgcttacag ctacgtgatg acggactgat actcaacgac
     1921 aacggcgggc ggagcattca ctttgagccg ctgctgccgg gggaggcggt gtacagccgc
     1981 agtgagtcaa tgtggctggt gcgcggtggt aaggcagcac agccggacgg ccatacgctg
     2041 gcgcggctgt ggggggcgct gcgccggata tccggttaag cccgcatctt tacctggcga
     2101 ccaacagcgc acaggggccg tggtggatac tggggggggc tgagcgggtg ccgggtgctg
     2161 aggacgtact gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggacgctg
     2221 acgtaccggc gtgaggccgc cggtgacctg gccggggaaa tcaccgacgt gacggacggt
     2281 gccgggcggg agttccgtct ggtgctgacc acgcaggcgc agcgtgcgga agaggcccgc
     2341 acctcttcgc tatcttcttc tgacagttcc cgccctctct cagcctcagc gttccccgac
     2401 acactgcctg gtaccgaata cggccccgac aggggtatcc gcctttcggc ggtgtggttg
     2461 atgcacgacc cggcataccc ggagagcctg cccgctgcgc cactggtgcg gtacacgtat
     2521 acggaagccg gtgaactgct ggcggtatat gaccgcagca atacgcaggt gcgcgctttc
     2581 acgtatgacg cgcagcaccc gggccggatg gtggcgcacc gttacgcggg aaggccggag
     2641 atgcgctacc gctacgacga tacggggcgg gtggtggagc aactgaaccc ggcagggtta
     2701 agctaccgct atctttatga gcaggaccgc atcaccgtca ccgacagcct gaaccggcgt
     2761 gaggtgctgc atacagaagg cggggccggg ctgaaacggg tggtgaaaaa agaactggcg
     2821 gacggcagcg tcacgcgcag cgggtatgac gcggcaggaa ggctcacggc gcagacggac
     2881 gcggcgggac ggcggacaga gtacggtctg aatgtggtgt ccggcgatat cacggacatc
     2941 accacaccgg acgggcggga gacgaaattt tactataacg acgggaacca gctgacggcg
     3001 gtggtgtacc cggacgggct ggagagccgc cgggaatatg atgaaccggg caggctggta
     3061 tcggagacat cgcgcagcgg ggagacagta cgctaccgct acgatgacgc gcacagtgag
     3121 ttaccggcga cgacaacgga tgcgacgggc agcacccggc agatgacgtg gagccgctac
     3181 gggcagttgc tggcgttcac tgactgctcg ggctaccaga cccgctatga atacgaccgc
     3241 ttcggccaga tgacggcggt tcaccgcgag gaaggtatca gcctttaccg ccactatgac
     3301 aaccgtggcc ggttaacctc ggtgaaagac gcacagggcc gtgaaacgca gtatgagtac
     3361 aacgccgcag gcgacctgac tgccgttatc accccggacg gcaaccggag cgagacacag
     3421 tacgatgcgt ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg cagtatggag
     3481 tacgatgctg ccggacgtgt catcagtctg actaacgaga acggcagtca cagcgacttc
     3541 agctacgatg cgctggaccg gctggtacag cagggcggct ttgacgggcg gacgcaacgt
     3601 tatcattatg acctgaccgg aaaactcacg cagagtgagg atgaggggct tgtcaccctc
     3661 tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag
     3721 cagtggcagt atgacggcca cggctggctg agagaaatca gccacctgag cgaaggccac
     3781 cgtgtcgctg tccactacgg ctacgacgat aagggccgcc tgacgggcga acggcagacg
     3841 gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca cgcctacaac
     3901 gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga gtggctgacc
     3961 tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt cgagtatacg
     4021 cgggacaggc tgcaccgtga gacggtgcgc accctcgcac cggacctgga catccgcatc
     4081 ccgtatgcca ccgacccggc gggaaaccgc ctgccggacc cggagctaca cccggacagc
     4141 acgctcacgg cgtggccgga taaccgcatc gcagaggatg cgcactatgt ttaccactat
     4201 gatgaatacg gcagactgac ggagaagacg gaccgcatcc cgacgggtgt gatacggacg
     4261 gacgacgagc ggacccacca ctacgacagc cagcaccgcc tggtgttcca cacgcggata
     4321 cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg
     4381 gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
     4441 ccggaggaga catggtacgg ctgggacggc gacaggctga cgacggtaca gaccgacacc
     4501 acacgtatcc agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca
     4561 gagaacggcg agcgggagaa agcgcagcgc cgcagcctgg cggagaagct gcagcaggaa
     4621 gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtgcggct gctggacaga
     4681 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggtgtg gcttgcgcag
     4741 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accagtgcga
     4801 aacgttcatc tttaccactg cgaccatcgg ggccagccgc tggcgctcat cagcgaagac
     4861 ggcgatacgg tgtggagcgg ggcgtatgat gagtggggca accagctaaa tgaggagaac
     4921 ccgcatcatg tgtatcagcc ataccgtctg ccggggcagc agtatgatga ggagtcgggg
     4981 ctgtactaca atcgtcaccg gtactatgat ccgttgcaga gacggtacat cacccaggac
     5041 cccattgggc tgaaaggtgg gtggaatctg tatcagtatc cgctgaatcc ggtgagcagg
     5101 atagatccgt tggggttatt ctcatgtgag agttgtagtc attttataga tgatataaat
     5161 aataaactgg ataaccttcc gtttaacccg acaaaaatgt ttacagctag tttaaatact
     5221 gctaattcag caaggctgta tgcaagtggc atgacaaaat tcggtataag tgttgctgcc
     5281 gatggaacga tcattgctgc cccagtcggt gcagcttcta tggctttagg ggcgtggaat
     5341 ataaaatctg cgacagctgc atataacaga gcaaatttac aattatcaga atcacttcag
     5401 gaaaatggta gagataggtg ttggagaaat ttattgggta ttttacctta tggtgagcag
     5461 tttgatgacc ctggtgaacc atcaatatta gacgtttatg gtgaaaagat tgagaatatc
     5521 aaagattctc caatgaagtt ttttgaagag cttgggacgc tggggttgta atgtgtattt
     5581 ttaatgatat ttttatgttt gtgtttattg ttagtagtat acttgtaacc gtgcattttg
     5641 tgtattcttt attttttaaa agtggaaagg taaaagataa ggtaaacaaa tggattaaag
     5701 taatgtgtga tttattttgg gggataggat agtggtgtga tatggataaa tataaattac
     5761 agtggctggc atatttcgac ctgacgccgg agtagataaa aaaacagaca ggattggagt
     5821 atagcaagcg tctgacaatc tctcgggaca gtagtatgat gaagaggcgg ggttgtacta
     5881 taaccgtaac cggtactacg atccgctaaa gggcaagtat atgac
//
LOCUS       DAZPLM010000235         5881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPLM010000235 DAZPLM010000000
VERSION     DAZPLM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.236"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            54..1043
                     /gene="ldhA"
                     /locus_tag="ACHVC7_003808"
     CDS             54..1043
                     /gene="ldhA"
                     /locus_tag="ACHVC7_003808"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR2117736.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1154..1576
                     /gene="hslJ"
                     /locus_tag="ACHVC7_003809"
     CDS             1154..1576
                     /gene="hslJ"
                     /locus_tag="ACHVC7_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR2117737.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1573..1839)
                     /locus_tag="ACHVC7_003810"
     CDS             complement(1573..1839)
                     /locus_tag="ACHVC7_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR2117738.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVALGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2113..5637
                     /gene="nifJ"
                     /locus_tag="ACHVC7_003811"
     CDS             2113..5637
                     /gene="nifJ"
                     /locus_tag="ACHVC7_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR2117739.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1495 a         1500 c         1492 g         1394 t
ORIGIN      
        1 gtaaaatatt tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac
       61 tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct
      121 ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca
      181 atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag
      241 agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg
      301 accttgacgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag
      361 aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg
      421 cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt
      481 atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc
      541 tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg
      601 aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc
      661 tgcactgccc gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga
      721 tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcag
      781 caattgaagc gctgaaaaat cagaaaattg ggtcgttggg tatggacgtc tatgagaacg
      841 aacgcgatct attctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc
      901 gcctgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag
      961 ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa aaaggcgaaa
     1021 cctgcccgaa cgaactggtt taatcttgcc gctcccctgc attccagggg agcttattca
     1081 gataatcccc aaagaccttt catcttctat tcttaaaata gccctgagtc agaaactgta
     1141 attgagaacc acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc tgatggcggg
     1201 atgtgtaagt aatgacaaaa ttgctgttac gccagaacag ctacagcatc atcgctttgt
     1261 gctggaaagc gtaaacggta agcccgtgac cagcgataaa aatccgccag aaatcagctt
     1321 tggtgaaaaa atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact
     1381 gtctaatggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc
     1441 gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga
     1501 tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc
     1561 ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg
     1621 tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga
     1681 aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa
     1741 acctagcgct acgtgcgccg cagtcgcctg ctgaacaggt tcactactac acgccgacaa
     1801 caataaagcg gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca
     1861 agccaaacga caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct
     1921 gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa
     1981 acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct
     2041 aaaattgaag gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg
     2101 taagggtgtc atatgattac tattgatggt aatggcgcgg ttgcttcggt ggcgtttcgc
     2161 accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct
     2221 gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccatg cgtggttgaa
     2281 atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt
     2341 tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacact gtacaaactg
     2401 gcaggcgaac tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca
     2461 ctctctattt ttggcgatca ttccgacgtc atggcggtgc gccagacggg ttgcgcgatg
     2521 ttgtgtgcgg caaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcgacg
     2581 ctgaaaagcc gcgtgccatt tattcatttc tttgatggtt tccgcacgtc ccacgaaatc
     2641 aacaaaattg tcccgctggc cgatgacacg attcttgatc tcatgccgca ggccgaaatt
     2701 gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgtgg tacgtccgcc
     2761 aatcctgaca cttatttcca gtctcgcgaa gccaccaacc cctggtacaa cgcagtctat
     2821 gaccatgttg aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg
     2881 tttgaatatt acgggcatcc gcaagctgaa cgggtgatta tcctgatggg ctcagccatt
     2941 ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt cggcgtgctg
     3001 aaagttcgcc tgtaccgccc cttctcagcc aaacacttgc tacaaactct accgggatcc
     3061 gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc
     3121 tatctggatg tgatgaccgc actggcagaa gcctttaata atggcaagcg cgaaactctg
     3181 ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta
     3241 ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt
     3301 tacgatgatg taaccaatct gtccctgccg ttgccggaaa acacccagcc aaactcggcg
     3361 aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac
     3421 aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac
     3481 tccaaaaaag cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt
     3541 tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat
     3601 aaatatcaga tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac
     3661 agcgcagatg aagtgtggtc gcgcttgccg caagaagttc aggccgtgtt aaaccagaaa
     3721 aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcaaatgtgg cctggcggcc
     3781 cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat
     3841 agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggccaggat
     3901 ctggtggaac gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg
     3961 ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct
     4021 gatttcgtga aaaccgtaac cgcagcgatg ctcgcagggc ttggtgatgc cctccccgtt
     4081 tcggcgctgc cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat
     4141 atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc
     4201 gcagcttgcc cacactcagc tattcgcgca aaagtggtac cacccgaagc gatggaaaac
     4261 gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat
     4321 gtcttgcagg tggctccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg
     4381 gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtcgcg cctggaacat
     4441 gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc
     4501 aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattcc
     4561 ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggc
     4621 gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc
     4681 tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt
     4741 gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc
     4801 gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg
     4861 aaatcagacg ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc
     4921 aacgatgttg ccgaagcaca tgaactgcta cgtgatgcag atgcactggt ggaaaaatca
     4981 atctggctga ttggtggtga tggctgggct tacgatatcg gctttggcgg tctggatcat
     5041 gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac
     5101 accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac
     5161 ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat
     5221 gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg
     5281 gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac
     5341 gatctggcac tcagccatga ccagatgcgc caactcacag ctaccggctt ctggccgcta
     5401 tatcgcttcg atcctcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc
     5461 ccgccgtcag aagcactgga agaaacgtta cttcacgagc aacgtttccg tcgtctgaat
     5521 tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa
     5581 cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg
     5641 ctctggataa ggattaccca attctaaaaa aaagcccgga cgactgttcg ggcttgtctt
     5701 tttatatgtt ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca
     5761 ataaaggcat ataacccgcg cagaataacg tatagaaaat taaaagtata atttttattt
     5821 tgtatgtatt atcttcgata cattcaccag acttattatc atttcagaag aattatttga
     5881 t
//
LOCUS       DAZPLM010000236         5865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPLM010000236 DAZPLM010000000
VERSION     DAZPLM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.237"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHVC7_003812"
     CDS             complement(<1..560)
                     /locus_tag="ACHVC7_003812"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR2117740.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHVC7_003813"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHVC7_003813"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR2117741.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHVC7_003814"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHVC7_003814"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR2117742.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHVC7_003815"
     CDS             complement(2371..3636)
                     /locus_tag="ACHVC7_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2117743.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHVC7_003816"
     CDS             complement(3863..5077)
                     /locus_tag="ACHVC7_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2117744.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHVC7_003817"
     CDS             complement(5177..5533)
                     /locus_tag="ACHVC7_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR2117745.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHVC7_003818"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHVC7_003818"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZPLM010000237         5839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPLM010000237 DAZPLM010000000
VERSION     DAZPLM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.238"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(39..401)
                     /locus_tag="ACHVC7_003819"
     CDS             complement(39..401)
                     /locus_tag="ACHVC7_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR2117746.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="ACHVC7_003820"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="ACHVC7_003820"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR2117747.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHVC7_003821"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHVC7_003821"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR2117748.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHVC7_003822"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHVC7_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR2117749.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHVC7_003823"
     CDS             complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHVC7_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR2117750.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="ACHVC7_003824"
     CDS             5322..5684
                     /locus_tag="ACHVC7_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR2117751.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1469 a         1444 c         1517 g         1409 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcattttta
//
LOCUS       DAZPLM010000238         5781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPLM010000238 DAZPLM010000000
VERSION     DAZPLM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.239"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHVC7_003825"
     CDS             <1..435
                     /locus_tag="ACHVC7_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR2117752.1"
                     /translation="TASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLR
                     EKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSG
                     EEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            446..1897
                     /locus_tag="ACHVC7_003826"
     CDS             446..1897
                     /locus_tag="ACHVC7_003826"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR2117753.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1887..2957
                     /locus_tag="ACHVC7_003827"
     CDS             1887..2957
                     /locus_tag="ACHVC7_003827"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR2117754.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            2957..4705
                     /locus_tag="ACHVC7_003828"
     CDS             2957..4705
                     /locus_tag="ACHVC7_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR2117755.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4755..>5781)
                     /locus_tag="ACHVC7_003829"
     CDS             complement(4755..>5781)
                     /locus_tag="ACHVC7_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR2117756.1"
                     /translation="LSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVA
                     KAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTG
                     KESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMD
                     KYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEE
                     REGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMV
                     DDYNSLR"
BASE COUNT         1331 a         1466 c         1494 g         1490 t
ORIGIN      
        1 actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt tctgaagcag
       61 ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt cctgcaaacc
      121 ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc cgtgccgcac
      181 ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc gcaggctatt
      241 gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg cgtgccgcaa
      301 agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat tatccaccag
      361 gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt gttaaatcaa
      421 accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg cgatcaccaa
      481 ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac agaaagcccg
      541 ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg aaaaccgctt
      601 atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc gtggcctgag
      661 cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca tctcccaggc
      721 gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc tttttgccgc
      781 agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga tgagccacct
      841 gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc tggtggcgct
      901 tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag gcgcgccgtc
      961 gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga tccccatcat
     1021 gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg cgtttctggt
     1081 ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg
     1141 cgcggtggtg ctggggctgg cgattggcta tttcgtcttc tggttccgca aagtgcgcct
     1201 tggcaaagca ttacaaccgc tgctcggttc aatgctgatc ccgttcgtta ctctgctggt
     1261 tttcggcgtg ctcacttact acgttatcgg gccggtgatg tccgacctca tgggcgggct
     1321 gctccacttc ctgaatacca ttcctccatc aatgaagttt gcggcagcgt ttctggtcgg
     1381 tgcgatgctg gcgttcgata tgggcggccc catcaataaa accgcctggt tcttctgctt
     1441 ctcactgctg gaaaaacaca tttatgactg gtacgccatc gtcggcgttg tcgcgttgat
     1501 gccgcccgtt gcggcgggtc ttgcgacgtt cattgcgccc aaactgttta cccgacagga
     1561 aaaagaggct gccagtagtg ccattgtggt cggtgccaca gtcgcaaccg aaccggctat
     1621 accttacgct ctggcagctc cgttgccgat gattaccgcc aatacgctgg cgggtggcat
     1681 taccggcgtg ctggtgatcg ctttcggaat caaacgtctg gcacctggtt tgggtatctt
     1741 tgacccgctg atcggcctga tgtcgccggt gggttcgttt tatctggtgc tagcgattgg
     1801 cctcgcgctg aacatttcat tcattatcgt cctgaaagga ttgtggttac gccgcaaagc
     1861 gaaagccgcg caacaggagc ttgtccatga acattgagtt actgcaacag ttgtgcgaag
     1921 ccagcgccgt cagcggcgat gaacaggaag ttcgcgacat tctgataaac acgctggaac
     1981 cttgcgttaa tgagatcacc tttgatggtc tgggcagctt tgttgcccgt aaggggaata
     2041 aaggtccaaa agttgccgtt gtcgggcata tggatgaagt cggctttatg gtcacccaca
     2101 tcgacgagag cggttttctg cgctttacca ccattggcgg ctggtggaat cagtcaatgc
     2161 tcaaccaccg ggtaaccata cgcacacaca agggatttaa aatccctggt gtgattggtt
     2221 ccgtcgcacc tcatgcgtta acggaaaagc aaaagcaaca gccgctgtca tttgatgaga
     2281 tgttcattga tattggcgcg aacagtcgcg atgaggtgga aaaacgcggt gtggcgatgg
     2341 gcgattttat tagcccggaa gccaattttg cctgctgggg cgaagataaa gtggtcggca
     2401 aggcgctgga taaccgcatc ggctgcgcaa tgatggctga actattgcag acggtgaata
     2461 atcccgaaat tacgctgtat ggcgtcggca gcgtggaaga agaagttggg ctacgcgggg
     2521 cacaaacctc ggcggaacac attaaaccgg atgtggtgat tgtgctggat accgctgtcg
     2581 caggtgatgt tccgggcatt gataacatta aatacccgct gaaactgggc caggggccgg
     2641 ggctgatgct gtttgacaag cgctacttcc ccaaccagaa actggtggcg gcgttaaaaa
     2701 gctgtgccgc acataacgat ttaccgctgc aattttccac catgaaaacc ggtgcgacgg
     2761 atggcgggcg ctacaacgtg atgggcggcg ggcgtccggt tgttgcgctg tgcctgccaa
     2821 cccgctatct gcacgctaac agcggcatga tttcaaaagc cgattacgat gctctgctca
     2881 cgttgatacg ggattttctg accaccttaa ccgcggagaa agtcaacgcg tttagccagt
     2941 tccgtcaggt ggattaatgc taaacgaacg ccagttaaag attgtcgatc tgctggagca
     3001 acagccgcgc acgcctggtg agctggcgca acagactggc gtttcaggca ggaccatcct
     3061 gcgtgatatt gactatctca actttaccct taacggcaaa gcccgcattt tcgccagtgg
     3121 cagtgcgggc tatcagctgg aaatcttcga gcgccgcagc ttttttcagt tgctgcaaaa
     3181 gcacgataac gacgatcggc tgctggcgct gttattactg aataccttta ctccccgtgc
     3241 gcaactcgcc tcggcgctta atttgccaga aacctgggta gcagagcgtc tgccccggtt
     3301 aaaacagcgt tatgaacgca cttgttgcct ggccagtcgc cccggtttgg gccatttcat
     3361 tgatgagaca gaagagaaac gcgttatctt gctggcgaac ttgctgcgca aagatccgtt
     3421 tttaattccg ctggcgggcg taacacgaga caattttcag catttatcca cggcctgcga
     3481 caaccaacac cgctggccgc tcatgcaggg tgattatctc tccagcctga ttctggcgat
     3541 ttacgccctg cgtaatcaac tgaccgatga gtggccgcaa tatcccggta acgagataaa
     3601 acaaatcgtt gaacagagcg gcatgtttct tggtgataac gctgtaagaa ccctgacagg
     3661 tttgatagag aaacagcatc agcaagcgca ggtaatttca gccgatcatg tgctggggtt
     3721 gctgcaaagg gtgccaggca tcgcgtcatt gaatattatt gatacgcagc tggttgagaa
     3781 tattaccggg catttattac gttgccttgc cgcaccggtg tggattgccg agcaccgcca
     3841 aagcagcatg aataacctga aagccgcctg gcctgcggcc tttgatatga gtctgcactt
     3901 tattacgcta ctgcgtgaac agctcgatat tccccttttc gatagcgatc tgatcggttt
     3961 gtattttgcc tgtgcgctgg aacggcatca aaacgaacgc cagccgatca ttttgctctc
     4021 ggaccagaac gcgattgcca ctattaatca gctcgccatt gagcgcgatg ttttacattg
     4081 tcgggtgatt attgcccgta gcttaagcga gcttattgcc attcgcgaag agattgagcc
     4141 gttattgatc attaacaaca gccattattt actggatgac gcggtaaata attacatcac
     4201 cgtaaaaaat atcattacgg ctgccgatat cgaacaaata aaacactttc tggcgacggc
     4261 atttattcgc caacagccgg aacgcttttt ctctgccccc ggaagttttc attattcgaa
     4321 tgtacgcggt gaaagctggc aacatattac ccggcaaatt tgtgcgcaat tagtggcaca
     4381 acaccatatt accgccgatg aagcgcaacg gatcatcgcc cgcgaaggcg aaggtgaaaa
     4441 cctgattgtt aatcgcctcg ccatcccaca ttgctggagc gaacaggagc gacgttttcg
     4501 tggatttttt attaccctcg cccaaccagt tgaggtgaat aacgaagtca ttaaccatgt
     4561 cttgatcgcc tgcgccgccg ccgatgcgcg tcatgagcta aaaatattta gctatctggc
     4621 aagcgtattg tgtcagcatc cggcagaagt tattgccggg ttaacaggat atgaggcatt
     4681 tatggagtta cttcacaagg ggtgaactgt gtcggatgcg ccgcatccga cactgcattt
     4741 atcatcaaaa acgtttaacg caggctatta tagtcatcaa ccatttcgtt gtactcgcgt
     4801 aacgctcgtg gaaatgagtc ttcgaccatc cagcttgtgt ttgcatcctg aagatgctct
     4861 ttatcccagt cagagtacgg gactttatct ctgacgcggc gaacatattt tttagtctcg
     4921 agttgatact ggtctgccga attaatgaac gagaaattca taccgccttt atccgcttct
     4981 ttggcctgcg ctaccagcgt ttccagttca gagactttct tcattgccgc atcaacatca
     5041 aacttctctt gcgaaatcag gttattaatt tgtttcgctg aaatcatgac tgccagagag
     5101 tagtagtgga atgttttccc ttctctttct tcaatatttt tcagttccgc aagctggcgc
     5161 ttatcattaa tttcctgaat cgccgcatga taagattctg ctacaggttc aaaggattcc
     5221 agatttttta agaatgtctg gtgcagcgtt ttccctttcg caaacgcatc gtctttataa
     5281 ttttcctggg tgtaatattt atccatttcg ttaatggtat tacccaacgc cacggcagca
     5341 tcaatataac tcaccgccac gccatcaata ggttgcagcg caggcgttaa cgccacagca
     5401 ctttttacgc ctttttcaca ctcagcgagg ttggattcac taatgccgta gatgccataa
     5461 acagtactct ctttaccggt aggcccctgt ttaaaatctt tcagccagtc agcataacgc
     5521 gccagactgt tctgtacagg gatctgtaac ttgttgtagc acttgatata gacatccatt
     5581 ttttcgtcga aaaccgcttg ttcatcgacc accggttgcg caggtgtttc aggtttagct
     5641 tctgcttttg caacgggagc ttcaggagct ggtgcggcag gttgactatt ggcaggcggg
     5701 ggcgtttctg tttcggcttt tttatcatca caacccgtca gaccgagggc catgatggcg
     5761 actataatag cggaagataa t
//
LOCUS       DAZPLM010000239         5693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPLM010000239 DAZPLM010000000
VERSION     DAZPLM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.240"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..828
                     /gene="yneK"
                     /locus_tag="ACHVC7_003830"
     CDS             <1..828
                     /gene="yneK"
                     /locus_tag="ACHVC7_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR2117757.1"
                     /translation="RHKENIEFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRV
                     FQTGVERVLFLFLNDFIEQFPMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLV
                     LNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGS
                     NKHVLPEVCICFLFLLAEQESPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASL
                     SDDKIIKHMARRTLELFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            978..2168
                     /gene="ydeA"
                     /locus_tag="ACHVC7_003831"
     CDS             978..2168
                     /gene="ydeA"
                     /locus_tag="ACHVC7_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR2117758.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2193..2858)
                     /gene="marC"
                     /locus_tag="ACHVC7_003832"
     CDS             complement(2193..2858)
                     /gene="marC"
                     /locus_tag="ACHVC7_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR2117759.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3070..3504
                     /gene="marR"
                     /locus_tag="ACHVC7_003833"
     CDS             3070..3504
                     /gene="marR"
                     /locus_tag="ACHVC7_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR2117760.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3524..3907
                     /gene="marA"
                     /locus_tag="ACHVC7_003834"
     CDS             3524..3907
                     /gene="marA"
                     /locus_tag="ACHVC7_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR2117761.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3939..4157
                     /gene="marB"
                     /locus_tag="ACHVC7_003835"
     CDS             3939..4157
                     /gene="marB"
                     /locus_tag="ACHVC7_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR2117762.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4214..5653)
                     /locus_tag="ACHVC7_003836"
     CDS             complement(4214..5653)
                     /locus_tag="ACHVC7_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR2117763.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     SRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1496 a         1402 c         1255 g         1540 t
ORIGIN      
        1 agacacaaag aaaatattga atttcaggta gtaataaatt taagccctat ttatttagat
       61 aattttcgcc atgatggaga aattaatatt ttttgcgctc ccaacccaaa gcctgtcact
      121 atggggcgcg tatttcagac cggcgtcgag cgtgtgctct ttctttttct gaatgatttt
      181 attgaacaat ttccaatgtt caaccctggc gttccgatca aaatagcgca tacaccacat
      241 attgaacccc tgcctccgga tcaccatacc gctgcagatt acttacgcca gtttgatttg
      301 cttgtcctga attttatctc tcgcggtaat tttgtcatac tcccccgatt atggaataac
      361 tctgaggttc acagatggtt cgtcaataaa gatcctaact tgatcaccgc cattctcgac
      421 ataacggaca gcgaattaaa agaggattta ttgcaaagcc tgatggattc attaggttct
      481 aacaaacatg tactacccga agtctgcatc tgctttttat tccttttagc agaacaagaa
      541 tctcctcatt ttcaggactt gtttttattt ttcgccaata tgttattgca ctatcaccaa
      601 tttatgaatc ccaatgaaag tgatttgaat gacgtgttga tgccagcatc attaagtgat
      661 gataaaatta tcaagcatat ggcgcgcagg accctcgaac tgtttgtaaa aaatgaaaca
      721 ccgccaaaag ttactcacga agacctggtg aaaaacaggc ctcgctcccc tgtcagacca
      781 cctatacccg caacctccaa aacgccagac ctccctgaac gtcattaaac cgtgatgtta
      841 ccgactctct gacgcggaac agaatcagcg tcagagaaac ggaaaacgcg atccagatca
      901 caaatacatt gtattcacat cattaaccgt tttaagatca tttcatcact ttttcgcaac
      961 tcacccgata atctgttatg acaacaaaca ctgtttcccg caaagtggcg tggctacggg
     1021 tcgttacgct ggcagtcgcc gccttcatct tcaacaccac cgaatttgtc cctgttggcc
     1081 tgctctctga cattgcgcaa agttttcaca tgcaaaccgc tcaggtcggc atcatgttga
     1141 ccatttacgc atgggtagta gcgctaatgt cattgccttt tatgttaatg accagtcagg
     1201 ttgaacggcg caaattactg atctgcctgt ttgtggtgtt tattgccagc cacgtactgt
     1261 cgtttttatc gtggagcttt accgttctgg tgatcagtcg cattggtgtg gcttttgcac
     1321 atgcgatttt ctggtcgatt acggcgtctc tggcgatccg tatggctccg gccgggaagc
     1381 gggcacaggc cttgagttta attgccaccg gtacagcact ggcgatggtc ttaggtttac
     1441 ctctcgggcg cattgtgggg cagtatttcg gttggcgaat gaccttcttc gcgattggta
     1501 ttggggcgct tatcaccctt ttgtgcctga ttaagttact tcccttactg cccagtgagc
     1561 attccggttc attgaaaagc ctcccgctat tgttccgccg cccggcattg atgagcattt
     1621 atttgttaac tgtggtggtt gtcaccgccc attacacggc atacagctat atcgagcctt
     1681 ttgtgcaaaa cattgcggga ttcagcgcca actttgccac ggcattactg ttattactcg
     1741 gtggtgcggg cattattggc agcgtgattt tcggtaaact gggtaatcag tatgcgtctg
     1801 cgttggtgag tacggcgatt gcgctgttgc tggtgtgtct ggcactgctg ctacctgcgg
     1861 cgaacagtga aatacacctc ggggtgctga gtattttctg ggggatcgcg atgatgatca
     1921 tcgggcttgg tatgcaggtt aaagtgctgg cgctggcacc agatgctacc gacgtcgcga
     1981 tggcgctatt ctccggcata tttaatattg gaatcggggc gggtgcgttg gtaggtaatc
     2041 aggtgagttt gcactggtca atgtcgatga ttggttatgt gggcgcggtg cctgcttttg
     2101 ccgcgttaat atggtcaatc attatatttc gccgctggcc agtgacactc gaagaacaga
     2161 cgcaatagtt gaaaggccca ttcgggcctt ttttaatggt acgttttaat gatttccagg
     2221 atgccgttaa taataaactg cacacccata cataccagca ggaatcccat cagacgggag
     2281 atcgcttcaa tgccaccctt gcccaccagc cgcataattg cgccggagct gcgtaggctt
     2341 ccccacaaaa taaccgccac caggaaaaag atcagcggcg gcgcaaccat cagtacccaa
     2401 tcagcgaagg ttgaactctg acgcactgtg gacgccgagc taataatcat cgctatggtt
     2461 cccggaccag cggtacttgg cattgctagc ggcacaaagg caatattggc actgggttca
     2521 tcttccagct cttccgactt gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga
     2581 aagagcatcc gaaaaccgat aaacgcgacg attaagccgc ctgcaattcg cagaccggga
     2641 atcgaaatgc caaatgtatc catcaccagt tgcccggcgt aatacgccac catcatgatg
     2701 gcaaatacgt acaccgaggc catcaacgac tgacgattac gttcggcact gttcatgttg
     2761 cctgccaggc caagaaatag cgcgacagtt gttaatgggt tagctaacgg cagcaacacc
     2821 accagcccca ggccaattgc tttaaacaaa tctaacattg gtggttgtta tcctgtgtat
     2881 ctgggttatc agcgaaaagt ataaggggta aacaaggata aagtgtcact ctttagctaa
     2941 ccttgcatcg cattgaccaa aacttgaacc gatttagcaa aacgtggcat cggtcaattc
     3001 attcatttga cttatacttg cctgggcaat attatcctct gcaactaatt acttgccagg
     3061 gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt gggtcgctta
     3121 atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc tccgctggat
     3181 attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg tattactccg
     3241 gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat gctggatcgc
     3301 ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg cggcgtactg
     3361 gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt agttggccag
     3421 gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac acttgagcat
     3481 ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac
     3541 tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg aatcgccact
     3601 gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc aacggatgtt
     3661 taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga tgacggaaat
     3721 cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac gatatggctt
     3781 cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc cgccgcataa
     3841 ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa atcattacaa
     3901 tagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt tcatccgcaa
     3961 tagcagctgc gcttattctc ttttccgcgc agggcgttgc ggaacaaacc acgcagccag
     4021 ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg
     4081 atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg ccctattata
     4141 atcaacacgc tatgtagtta ttgtcaggct ctgatcacag agcctgacat ctccgtaata
     4201 tcttcctcaa gtctcaaata ttttcgccat tgttggcgat gagatcccga taccagtaaa
     4261 acgacgcttt acgtatccgt tccagcgttc ccttcccttc gttgtcttta tcgacataaa
     4321 tcattccgta gcgctttttc atttcccctg ttccggcaga aaccaggtca atgcagcccc
     4381 acggggtgta gccaattaag tcaacaccat cttcaactac ggcttttttc atttcccgaa
     4441 tatgggaagc aaagtaatca atgcgatagt gatcgttcac cgtgccgtca gcttgtctct
     4501 ggtcaaccgc accaaatcca ttttcgacaa taaacagcgg caactggaaa tgatcccaga
     4561 accagttgag tgaataacgt agccctgccg gatcaatttg ccatccccat tcggatgtat
     4621 cgatataagg gttgctgacc aaatctcgtg attcaacata atccagttgc gggttatcgg
     4681 gagaaaattg tgtcgtaaaa gacatgtagt agctaaaacc gataaagtcg acacatccac
     4741 tggcaagaat cgcgttatct tctggtgtga tatcgaggtt gaatcctttc ctggcaaagt
     4801 aattcagcat atgttgcgga taatatccac gagcatgaac atcagtaaac cagtaacgac
     4861 gatgcatcgc tttcgtggcc atcatcatat cgttgggtgc acacgtcaga ggatagatgg
     4921 ggcacatggc gatcatacag ccaatgttaa attcaggatt gatcgatttt ccagtctgta
     4981 ccgccagggc actggcaact aactcgtaat gtaccgcctg gtacattatt tgttcgcgct
     5041 cattgatatc ttcctctggc gaatacaaga taccggaatt agtaaatgga cacaggcttt
     5101 cgctgaaatt cacctgatta ttgatttcgt taaacgtcat ccagtacttt actttttctt
     5161 tatagcgcgt gaagaccgtt gatgcgaagc ggataaaaaa gtcgatcagc ttacggtttc
     5221 gccagccacc atattttgtc accagatgat aaggcatctc aaaatgcgaa agcgtcacca
     5281 caggttccat tccttgcttc aggcattcat cgaacagatc atcataaaat tgtaaaccct
     5341 cttcattcgg ctcctgttcg tcaccttgcg gaaagattcg tgtccaggca atggaagttc
     5401 gaaagcattt gaatcccatc tcggcaaata actgaatatc tgttttatag cgatgataaa
     5461 aatcaattgc ttcatgattg ggataattaa gcccgtcgat aacgccttct gtcacttcac
     5521 gcggcacccc gtgagcgcca gcagtcatca catcagcgat actgatgcct tttcctcctt
     5581 cattccagcc accttccaac tgatgcgcgg ctaccgcgcc accccataaa aaaccttttt
     5641 taaatcctga catacacaac tccttaacta aaatagatta ccaccagact tcc
//
LOCUS       DAZPLM010000240         5675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPLM010000240 DAZPLM010000000
VERSION     DAZPLM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.241"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            12..635
                     /gene="nfeR"
                     /locus_tag="ACHVC7_003837"
                     /gene_synonym="yqjI"
     CDS             12..635
                     /gene="nfeR"
                     /locus_tag="ACHVC7_003837"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR2117764.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(742..2262)
                     /gene="aer"
                     /locus_tag="ACHVC7_003838"
     CDS             complement(742..2262)
                     /gene="aer"
                     /locus_tag="ACHVC7_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR2117765.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2680..4059
                     /gene="ygjG"
                     /locus_tag="ACHVC7_003839"
     CDS             2680..4059
                     /gene="ygjG"
                     /locus_tag="ACHVC7_003839"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR2117766.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4101..4433)
                     /locus_tag="ACHVC7_003840"
     CDS             complement(4101..4433)
                     /locus_tag="ACHVC7_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR2117767.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4652..5635
                     /gene="ebgR"
                     /locus_tag="ACHVC7_003841"
     CDS             4652..5635
                     /gene="ebgR"
                     /locus_tag="ACHVC7_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR2117768.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1374 a         1544 c         1434 g         1323 t
ORIGIN      
        1 aggaggctga tatgagccat catcacgaag ggtgttgtaa acatgaaggc cagccacgcc
       61 atgagggctg ctgcaaaggt gagaagtcag aacacgagca ctgcggacac ggtcaccagc
      121 atgaacacgg tcaatgctgc ggtggtcgcc acggtcgcgg cggcggtcgt cggcaacgtt
      181 tctttggtca cggtgaattg cgtctggtga ttctggatat tctctcgcgc gatgacagcc
      241 acggttacga attgattaaa gcgattgaga atctaaccca ggggaattac accccaagcc
      301 cgggcgtcat ctacccaacg ctggattttc tgcaggagca gtcgctgatt accatccgcg
      361 aagaggaagg tggtaagaag cagattgcgc tgaccgaaca aggcgcgcag tggctggaag
      421 aaaaccgcga acaggtggag atgattgaag aacgcatcaa agcgcgttgc gttggtgctg
      481 cgctgcgcca gaacccgcaa atgaagcggg cgctggataa ttttaaagcg gtgctggatt
      541 tacgcgtcaa ccagagcgat atcactgatg cacaaataaa aaagatcatt gcggtgatcg
      601 accacgccgc ttttgatatt acccaactgg attaatcacc gattgccgga tgcggcgtac
      661 cctacacacc ccgcaggcct gataagatgc gccagcatcg catcaggcat tgtgctccaa
      721 ccgccggatc cggcataccg attaatgcag caccgtcacc gcgtcttcca gtcggctggc
      781 gcggtgtttc accatcgccg acacctgcgc actctcttcc accagctcag catttttctg
      841 ggtgatcagg ttaagctcat ccactgcacg ggtcaggctg gaaagcccat cggcctgttc
      901 cagcgttgaa tggctaatct gggcgatcaa ctgggtgacg tttttcacct gtgccacaat
      961 atcttccatc gtccgtccgg cggcgtgtac ctgctgcgaa ccggattgca ccttatcagc
     1021 actggcatca atcagcttgc gaatatcgtt ggcagcatta gcgctgcggc tggctaaatg
     1081 acgcacttcc cctgccacca ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc
     1141 caccgccgca ttcagggcca gaatattggt ctgaaacgca atatcgttaa tcagcgaagt
     1201 aatggtgcca atgcgctggg tactgtcggc gatatcgtcc attgtcttga tcaccgtggt
     1261 catcgcctcc ccaccctgca ctgccgcatt actggcggtg atagaaagtt tatcggcagc
     1321 cgacgccgtg gcagagttct gtttcaccga cgccgccatt tggttcatgg tcgccaccgt
     1381 ttgctgaacg ttatcaactg tctgctgggt atgttcgttc agttcatcgg tgcctttcgc
     1441 cagcgtctca ctgccatttc tgacactgga cacctggctt gagacatcgt taattagcca
     1501 acggcacatc aggccaagct gccctaccgc gcgtaatgtc agccccagct catcgctgcg
     1561 attcaggtgc tcaacactat tacgctctcc ggtcgccacc ttcagtgcct gacgggcgac
     1621 attttctatc gggcgcacaa tctgccattc aaaacaagcg cttgccaaca ataccactaa
     1681 cacacagagg aaatacgtca ccaccggggc agcaacaaac caaagcatgg ccgccagcaa
     1741 gataaacatc agggtcatca ctccacgcgc ccgccagcga agcggtaatg aaggcagttt
     1801 acccagccag cctttacgca ccaccaggcc tttatgaata cgcttactgg tacgtccggc
     1861 gttcagcgct ttgtacagcg gctccaccgc cgcaatctct tcatccgtcg cccgggtacg
     1921 aatcgacata tagccactga tttttccctc gcgcaccatc ggtaccgcat tggcccgcac
     1981 ccaataatgg tcgccatttt tgcggcgatt tttcacgatg ccgctccagg gctccccttt
     2041 tttcagggtg aaccacatat ccgcaaacgc cgcttttggc atatccgggt gacgcaccat
     2101 gttgtgcggc tgcccttgta actcttgcaa ggtaaagccg ctcacctgca caaaagtgtc
     2161 attagcatga gtgatatagc tttgcagatc ggtagtggac atcagagtgg tatcgtccgc
     2221 cagcggggta ttttgctggg tgacatacgg atgagaagac atgttcgcgt cctgtgcagg
     2281 ttatatggtt gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac
     2341 cgattaattt gatttagatc gcaatttgcg attaaagcac aaatctaatt ctttgattta
     2401 aaatactttc actctgttac tatacgaaaa cgttaattat cttgcccaaa aatcaggcaa
     2461 ttattgccct gaaaacgtgc atttgcgcag caatcatcaa atccataccc gacaaaaacc
     2521 gtgcaaaata acaacaaatg ttaacagata gcattaaata ttgcacaaat gataaccgaa
     2581 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     2641 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     2701 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     2761 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     2821 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     2881 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     2941 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     3001 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     3061 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     3121 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     3181 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     3241 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     3301 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     3361 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     3421 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     3481 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     3541 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     3601 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     3661 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     3721 aatgtgttgc tggagcagaa cttaccggct caggctgatc aaaaaggcga tatgttgctg
     3781 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     3841 gggatgttga tggcgattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     3901 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     3961 gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     4021 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     4081 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     4141 gttaacagca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     4201 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt ttttcccatc
     4261 agctcctctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     4321 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     4381 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgcttcc
     4441 tcccttcaag taaaatttta ctaaactata gcaaaatttt ttctcaatcc tgtaggctaa
     4501 aaatggagaa tgcaggcgtg atcacattcg taagccgctg tgttaccgtt acagcgtcaa
     4561 agaaacgcgc tttatttact gaaaacaggt gacccgataa gcacttcctc tacaatgggg
     4621 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ttaaaagaca tcgcaatcga
     4681 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     4741 gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     4801 tagcgcccgt aaactacaga caggcgcagt caaccaacac catattctgg ctatctacag
     4861 ctaccagcag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     4921 aactcagtgc gaaaagctgg cgatcgaact gaccaactgt tatgaacaca acggattacc
     4981 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg ccctgcgcgc
     5041 cgctgccagc gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     5101 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     5161 caaccagggc gttaatcgta ttggttttat tggcggtgaa gatgagcctg gcaaggcgga
     5221 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     5281 tatctggcgc ggcggttttt ccagttcgtc gggttatgaa ctggcaaaac aaatgctggc
     5341 gcgggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     5401 gcgggcaatt catgaacgtg gcctgaacat cccacaggat atttcgcttt tcagcgttaa
     5461 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     5521 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     5581 gccgctgcta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     5641 cttacacact gtccggcaat catttttgcc ggaca
//
LOCUS       DAZPLM010000241         5670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPLM010000241 DAZPLM010000000
VERSION     DAZPLM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.242"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..663)
                     /locus_tag="ACHVC7_003842"
     CDS             complement(<1..663)
                     /locus_tag="ACHVC7_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2117769.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
     gene            763..1353
                     /locus_tag="ACHVC7_003843"
     CDS             763..1353
                     /locus_tag="ACHVC7_003843"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR2117770.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1435..2175)
                     /gene="pflA"
                     /locus_tag="ACHVC7_003844"
     CDS             complement(1435..2175)
                     /gene="pflA"
                     /locus_tag="ACHVC7_003844"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR2117771.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2367..4649)
                     /gene="pflB"
                     /locus_tag="ACHVC7_003845"
     CDS             complement(2367..4649)
                     /gene="pflB"
                     /locus_tag="ACHVC7_003845"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR2117772.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4704..5561)
                     /gene="focA"
                     /locus_tag="ACHVC7_003846"
     CDS             complement(4704..5561)
                     /gene="focA"
                     /locus_tag="ACHVC7_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR2117773.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1461 a         1334 c         1412 g         1463 t
ORIGIN      
        1 atccagaatg atattccctg cgacagcaat ttcctgctcc tttgcaaatt gccagtggaa
       61 aggtttgccg ctgggataac gaaatacaac acattgatga ttaagcagat catgaggatg
      121 gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata atttcactgg
      181 tgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc gaacgccagc
      241 gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct cgactttaat
      301 atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac gtgcagctac
      361 ccgggcggca ttgattttga gcgttcctgt tggtgtcaga cgaaaatcat tcatctcatc
      421 taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat tagaacccgc
      481 ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa gacgttgttc
      541 cagtgtttta atactatggc taatggcgga cgaggataag cctaactcat cgcccgctgc
      601 acgaaagctt tgattacggg ccacagcaaa gaaagtggca aagtcagaca tattcatccg
      661 cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacatg attatgccgc
      721 ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt
      781 tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc tgatgcgatc
      841 catcagcggg caatggagcg gaaaatacag gtgacggaac ttgatttata tcggcgtaat
      901 ttcaacccgg tgatgacgcc ggaagatgaa ccagactgga agaatatgga taaacgttat
      961 tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt agtggtggtt
     1021 tttcctgtct ggtggtacag cttcccggca atgctaaaag gatatattga cagagtatgg
     1081 aataatgggc tggcttatgg agatgggcag aaattaccat tcaataaagt tcgttgggtg
     1141 gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa aaatataagc
     1201 gattatttaa aaaatatgtg cagttatctt ggtattgaag atgccgatgt cactttcttg
     1261 tgtaatacag tggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta
     1321 tctcaggtac gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat
     1381 gaagtgcgga ataaaaaagc ggcaactcaa taaagttgcc gctttacggg gaaattagaa
     1441 cattacctta tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt
     1501 cggtggttta acaccgtcga gtttgtactc ttcacccatt gccacccatt tgtgtttgcc
     1561 cagctcgtgg taggggagaa gctcgatttt ctcaacgttg cccatatcac gggtaaattc
     1621 accgaggcga tgcgctgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat
     1681 ccacaccttc acatttttgt tcgccagata tttggcgaac tccagcgtgc ggtgattgga
     1741 aactccaacc agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag
     1801 gtcggttact tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt
     1861 gtccagacag gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac gaacaaactc
     1921 agcttgtagg attgcctcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa
     1981 gtggcgatag gtcaccacct ctttcattaa atcttcaacg gtaacttctt taccgccatg
     2041 cgtatcccag gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa
     2101 ggtgataaag cgaatacccg ggccgtctac ggttccacag gattcaaagg agtgaatgcg
     2161 accaataact gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc
     2221 gcagctcgaa ggctacgtcg agtctgtttt ggcagtcacc ttagagtata gatagctgac
     2281 aaaaaaggct ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt
     2341 tactgtacga tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg
     2401 tcctgctgct gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg
     2461 gtcagctgcg gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc
     2521 acgttaacgt tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc
     2581 atcagaccag ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg
     2641 atagagaagg tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa
     2701 gtcagagagg ctactgcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg
     2761 aacggcgcgc cagcacgacg accgtctggg gtgttacccg ttttcttacc atacacaacg
     2821 ttagaagtga tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc
     2881 tgaattttct tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatcg
     2941 ttgttaccaa actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg
     3001 tcacgaatcg gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac
     3061 agaccagcga taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga
     3121 gaggcttcgt agctgtactt gtcgtgcatg tagtggatga tgtttagtgc agtgatgtac
     3181 tgtttagcca gccagtccat gaagtgatcc atgcgctcca tcacttcatc atagttcagg
     3241 acatcgcctt tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg
     3301 ccgccgttga ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc
     3361 atttgtttac caacgatcat cgggcttacg cagcaagcaa tagcgtagtc atcgttgttg
     3421 aagtccggac gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact
     3481 ttagcggcga atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc
     3541 ggttccggag acggacccat ggtgtacagg gtgttcagga aacggaagct gtttttggta
     3601 accagggtac gaccgtcgag gcccatacca ccgatagatt cggttgccca gatcgggtcg
     3661 ccagagaaca gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg
     3721 accaggtggt caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca
     3781 cgttcgatgt acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc
     3841 tgagacttaa cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg
     3901 gtagccggac cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga
     3961 gcgcggtgct gttcagcgat ttcttcgcgc agacggatag tctgttccaa gtttacgccg
     4021 ttttccagat cagcctgcag agaagtgaac tgtgccagtt tgtctttcat caggtagtcg
     4081 ataccgtaca gcgcaacgcg acggtagtca ccgatgatac ggccacggcc atatgcatct
     4141 ggcagaccgg tcagaacacc agatttacgg caacgcagga tgtccggagt gtaaacgtcg
     4201 aacacgccct ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcggatcc
     4261 agttcgcggt tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata
     4321 agagcacgtt tcagcggagc ttcagtctgc agaccaacga ttttctcaag ctgcttgttg
     4381 atgtagccag cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa gtcaactggc
     4441 gcgtgagtgc ggttttccag tttaacgcct tccattactt tgtcccacag ggtggtggtc
     4501 gcttcagtag cgccagccag gaaggactcg tcaccctcgt acggagtgta gtttttctga
     4561 atgaagtcac ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct
     4621 gtggctaact tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta
     4681 tttactgcgt acttcgacaa ccattaatgg tggtcgtttt cacgcaggta aatgacccag
     4741 tatgtcaacc caaccaacaa accaccaccg ataatgttgc cgatcgtaac cggaatcagg
     4801 ttatcagtga tgaaattcat cacggtcagg tgagaaaaat tttccggtgc agaaccgact
     4861 gcggtccaaa attccgggga tgcgaagtcg cggattacaa tacccatcgg gatcataaac
     4921 atgtttgcga tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata
     4981 atgaacgctt tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc
     5041 atcaggtttg ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg
     5101 tcggcggttt gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa
     5161 agccacatta aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc
     5221 cagtttttcg ccaactgacc ccaggtgatg cgcccactcg ccttagcaac aacaatcaac
     5281 acggtggaag taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag
     5341 caaatgccgc caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct
     5401 gtgatataga agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa
     5461 gtcttaagcg gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt
     5521 gcagcaggaa gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta
     5581 agtcggcgac gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc
     5641 gcctggctta taggcccgta actcgcatgg
//
LOCUS       DAZPLM010000242         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPLM010000242 DAZPLM010000000
VERSION     DAZPLM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.243"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHVC7_003847"
     CDS             complement(<1..513)
                     /locus_tag="ACHVC7_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR2117774.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHVC7_003848"
     CDS             complement(759..1211)
                     /locus_tag="ACHVC7_003848"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2117775.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHVC7_003849"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHVC7_003849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR2117776.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHVC7_003850"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHVC7_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR2117777.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHVC7_003851"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHVC7_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHVC7_003852"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHVC7_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR2117778.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHVC7_003853"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHVC7_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR2117779.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPLM010000243         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPLM010000243 DAZPLM010000000
VERSION     DAZPLM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.244"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHVC7_003854"
     CDS             complement(104..418)
                     /locus_tag="ACHVC7_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR2117780.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHVC7_003855"
     CDS             complement(423..638)
                     /locus_tag="ACHVC7_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR2117781.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHVC7_003856"
     CDS             complement(789..2642)
                     /locus_tag="ACHVC7_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR2117782.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHVC7_003857"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHVC7_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHVC7_003858"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHVC7_003858"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHVC7_003859"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHVC7_003859"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHVC7_003860"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHVC7_003860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHVC7_003861"
     CDS             complement(3908..4966)
                     /locus_tag="ACHVC7_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR2117783.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHVC7_003862"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHVC7_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHVC7_003863"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHVC7_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2117784.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPLM010000244         5581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPLM010000244 DAZPLM010000000
VERSION     DAZPLM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.245"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..449
                     /locus_tag="ACHVC7_003864"
                     /pseudo
     CDS             1..449
                     /locus_tag="ACHVC7_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(491..943)
                     /locus_tag="ACHVC7_003865"
                     /pseudo
     CDS             complement(491..943)
                     /locus_tag="ACHVC7_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1007..1153
                     /locus_tag="ACHVC7_003866"
                     /pseudo
     CDS             1007..1153
                     /locus_tag="ACHVC7_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1153..2310)
                     /gene="intS"
                     /locus_tag="ACHVC7_003867"
     CDS             complement(1153..2310)
                     /gene="intS"
                     /locus_tag="ACHVC7_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR2117785.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2472..2546)
                     /locus_tag="ACHVC7_003868"
     tRNA            complement(2472..2546)
                     /locus_tag="ACHVC7_003868"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2511..2513),aa:Arg,seq:cct)
     gene            complement(2622..3554)
                     /locus_tag="ACHVC7_003869"
     CDS             complement(2622..3554)
                     /locus_tag="ACHVC7_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR2117786.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3848..4603
                     /gene="mlaA"
                     /locus_tag="ACHVC7_003870"
     CDS             3848..4603
                     /gene="mlaA"
                     /locus_tag="ACHVC7_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR2117787.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4785..5581)
                     /locus_tag="ACHVC7_003871"
                     /pseudo
     CDS             complement(4785..5581)
                     /locus_tag="ACHVC7_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1370 a         1291 c         1249 g         1671 t
ORIGIN      
        1 cactgtgctg aatacataga aatttttgat gagtctattg cttttacatg tctctctgca
       61 ttttcttctg aaaaacaatt tggagtattt ctgtttggat gtttgaaaag cacagggcta
      121 aagttaaaac gattattcat acggatttat ctgcaccatg gcgtcttaag gatatatcat
      181 caagattata tctcagcgaa agtttactaa agatgaaatt gaaagaagag ggggtatcat
      241 tcagtaagat catacttgat gagaggatgc aaatggctga atatttactc agcactcgtt
      301 gttatcctat tagtaaagta gctaaggtct gtggttatgc cagtgtctca tactttactt
      361 atgtatttag acgttatttt ggtgtttctc caagtcaata ctctcagagg agttcagaaa
      421 gtaaaattct tactcaccag ggaatctgat cattgttctt gcccccttat ttccagacag
      481 ggggtgtatc ttaagttaac gttacccgct gacgtcgata ttctcgcgga gagcgataac
      541 ccagcgcact gtgcggatgg ttttcattgt aatgttcgat cgccactgca agattatgca
      601 atgccgttct tacattcggt ttcggcatga acgcgatgta gtcttccttc atcgttttca
      661 cgaacctttc tgccattcca ttactctgag gactgctgat tgccgttgtg cagggctcca
      721 gattcaactc tctggcgaac tgccgtgttt catgcgctct atacgctgaa ccgttatctg
      781 tcagccactg gatggactgt tccggtactt tatcgccaaa gcgtttttct atcgctccta
      841 acatgacatc ctgcactgtc gctttatcgt aacctcccgt gcttgcggcc cagtctatgg
      901 cttcacgatc gcaacagtcc agggcgaacg taacacgcag ttttgaatcg ccacgggttt
      961 aacagacacc tcagagtcat ttaagatggc ttaaagagag gtgcccatga gcggtaagcg
     1021 ttatcccgaa gagtttaaaa ctgaagcagt caaacaggtt gttgatcgcg gttattctgt
     1081 tgccagcgtt gcaacacgtc tcgatatcac tacccacagc ctttatgcct ggataaagaa
     1141 gtacggtccg gattactcca ctttttcatc aagccaatcc gcccaccact gcatcatttc
     1201 tctgcgctta tcgagatact gagcatggtt gtaaataccg cgcacagatc cgccgttggc
     1261 atgtgccagc tgcacttcaa tagcgtcagc aggccattcg tgctcgttca taatcgtgct
     1321 gaattcatgc ctgaatccgt gaccgctttc cagaccttca tagccgattt gtttgatcac
     1381 aagcaatacc gcgttctcgc agattggctt cttcttatcg ttgcgcccgg caaaaacaaa
     1441 ctctgagact ggtttggtga ttgagcttag cgtagtgaga agttcaacca cctggtctga
     1501 catcgggacc acatgaattt tgcgtccctt catcacactg gcgtcgatgg tgataatcct
     1561 gttttcaaaa tcgacgttct tccatagcat ggaacgaagc tcttttgttc ttagggctgt
     1621 gtagcgtaaa actttggtcg caatgagcga tacgatactt cctgaaaatg ttgccagtgc
     1681 tttgttgaat gcaggcatct ggtctgctgg aaggaacggg aagttcttct tgcggtatcc
     1741 tttcatggcg tctgcaaggt caggtgccgg gttatattta gcccttccgg tgacaatagc
     1801 gtaacgaaaa acctcgccgc atcttctgcg ggctttgttg gctcgctcca ttgcaccgcg
     1861 atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct gaatctcaag
     1921 accgccgatg atgggtaaaa tgtcgtcatc aaacattttg gcaagttcag ttgcatagcc
     1981 tactgaccag acttgcttct tgtgctcgta ccattccttg taaatcgcac taaatgaatt
     2041 gttgttagac gaagcctttt tcgcctttac cggaacgatg ccaaccgaga tgtctttcct
     2101 cgcagtccat gccttatccc ttgcttcctg caaagtcata agcggatatt ttccgacggt
     2161 caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt tcccggatac
     2221 aggaacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt ctttcggctt
     2281 tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta aaattatttt
     2341 atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa ccatgacgaa
     2401 ccgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac ggatgggagc
     2461 gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt aggaggggct
     2521 cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta atggagtgcg
     2581 gggtaagtac gctgccgctt gattgcttaa accctcgcca tttatgccgg gtttttatca
     2641 tttttcttaa tgttttccgc acgttctgct ttttggcgtg cttctgcttt acgcttgttg
     2701 ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt gccgccgcag
     2761 atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca gtgcagcgta
     2821 ccgttaaaca ccagatagag gatttcaaca gaaccgacca cgatatgggt ggtgtcacct
     2881 agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc tgctgcagga
     2941 aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc aaacatctcg
     3001 ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg agtttcttca
     3061 ttgaagatag gcatatattc aaatgcccac gcagcaatac cagtcccgag aatattaccc
     3121 agcagcacga ctccccataa ccgcataagt aagccaacgt tgctcattgt cggtttttgc
     3181 atgacgggta gtaccgcagt cacggtgttt tcggtaaata attgctggcg ggccattatg
     3241 acgataataa aaccaaaggt ataaccgaga ttctccagta agaagctgcc cggcacacct
     3301 tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga cagacccgcc
     3361 gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc accatcctgg
     3421 cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc tatttctatt
     3481 tttttgccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt
     3541 ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc gtagcggctt
     3601 ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggctgcg tttgcctttt
     3661 tctgtttcta tagaatcaag tagcctacag ggcggcgatt accaggctat gatcaaatca
     3721 gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac acctaaacag
     3781 gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa aaaacaggga
     3841 gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc tggtggggtg
     3901 tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt tcaaccgcac
     3961 catgtacaac ttcaacttca acgtattaga cccgtatatt gttcgaccgg tcgctgtcgc
     4021 ctggcgtgat tatgttccgc aaccggcgcg taatggtttg agcaacttta ctggcaacct
     4081 tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc aggggatggt
     4141 ccactttacc cgctttttcc tgaacaccat tttggggatg ggcggcttta ttgatgttgc
     4201 agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta gtacgcttgg
     4261 tcattatggc gtgggttatg ggccttacgt tcagttaccg ttctacggta gcttcacgct
     4321 gcgtgatgac ggtggtgata tggcggatgg tctttacccg gttctttcct ggctgacctg
     4381 gccgatgtct gtgggtaaat ggacgcttga agggatcgaa acccgcgctc agctgctgga
     4441 ttccgatggt ctgctgcgtc agtcgtccga tccttatatt atggtgcgcg aagcgtactt
     4501 ccagcgtcat gatttcatcg ctaatggcgg cgaactcaaa ccgcaggaaa atccgaacgc
     4561 acaagcgatt caggatgatt taaaagatat tgattctgaa taagaaacaa ataaaaaagg
     4621 tgagtcgcaa aactcacctt ttttgttgtt atccctcaat taccacaagt aaaaggagat
     4681 aaaccgacaa tgacccggac gtaatgatgt aaaaatactg atgttcgtca ctgactattt
     4741 ccgatattca ggcgttgaaa aatcaaaaaa ctggaaaaat aattttaaat atcatcaaaa
     4801 ttaacacctg gcgcagatag ttgattaatt tcattttgat atttcgcctg gcgattttga
     4861 agtctttttt caacgttatc ttcgagatcc ttaaagtctt tgtatttatt ttcaggatgg
     4921 tcaacgtcaa ctttgttagc gaataaatta gaaattattt cttttacatt gtcgcgtcta
     4981 aattttgcat cgtccacttg aaaattatat cttattgctc gacctaaacg tttaaaaact
     5041 tcgttaggtt ctctcagtct ttccccgttg agctcgttta caacctgatg atcggaacct
     5101 gttgcaaatg attctgccat gatagcttta ctgctttcaa accacgctga agcattgctg
     5161 aagcattcct ggctttttca ctacaatatt gggtgagact atcaccatta atacgaattc
     5221 ctctttgaat gtggtagtcg tcctgatcgt agtaaccgag agataagtaa tcatttccgt
     5281 ttttattttt aattacaata gcgagagagt tgcctttttt atctttaccg taccaggtaa
     5341 ataagtccgt tggttcgcta ttcccgctct catcaaattc cgttaccgta tgtctgagtt
     5401 tgtgtgatat cttatcgaag ataaaaccag gatctccatc atatatttct tttaatgcgc
     5461 tgaagatcat ttccttgtta ttgatatcat gagtcttctc aactatgttg tcattaatga
     5521 tagtacggac gagttctttg tctttatagt tcgcaggtaa ttctattggt tcttggttgt
     5581 c
//
LOCUS       DAZPLM010000245         5548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPLM010000245 DAZPLM010000000
VERSION     DAZPLM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.246"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHVC7_003872"
     CDS             complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHVC7_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR2117788.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1777..3048)
                     /gene="efeB"
                     /locus_tag="ACHVC7_003873"
     CDS             complement(1777..3048)
                     /gene="efeB"
                     /locus_tag="ACHVC7_003873"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR2117789.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3054..4181)
                     /gene="efeO"
                     /locus_tag="ACHVC7_003874"
     CDS             complement(3054..4181)
                     /gene="efeO"
                     /locus_tag="ACHVC7_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR2117790.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4239..5069)
                     /gene="efeU"
                     /locus_tag="ACHVC7_003875"
     CDS             complement(4239..5069)
                     /gene="efeU"
                     /locus_tag="ACHVC7_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR2117791.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1393 a         1518 c         1308 g         1329 t
ORIGIN      
        1 ataatattat ccctaaagaa taaatcagag gaatatattc ggtgttaaac agtcagtcaa
       61 agtatagccc attgttgatt aaacctcata agcaaaattg gcaccagatc tcctgtacag
      121 gttgaatggc cgaaaaaaga taggcagatg ctcatttttt tggcttctgg aagaaaaact
      181 ctccgcgctg gcagtattac actggtctga tgctatgacg aaaaatgcct ggtaagagta
      241 tctaaaccag actccagttc agcttatcaa cacgctcaaa gtcgcccccc tgaacgctaa
      301 cctgaagtga aaatctgttg aaataaaaca aagcccggtt cgcccgggct ctgcaccgat
      361 aacacactta actgtaggca tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct
      421 ctttaccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      481 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      541 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      601 gcgcctcgtc aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg
      661 gcgcaatttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      721 agcgccggac cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      781 ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
      841 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
      901 cgcccccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt
      961 gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac
     1021 caatggcttc tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
     1081 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
     1141 ctttttgtct tcccatagag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
     1201 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
     1261 ttggataaaa tgccgaaaaa cggctacgcg caccgtttac ggcgtcggta acacatgaaa
     1321 agaaaggatg aggttgaaaa tgcaaagtga cgagatgact accggaggag aaaactccgc
     1381 gagtggtggc gcgttgatta tctaaaacat gtccagtaca ggacgttacc atccgcgatc
     1441 tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacaga
     1501 tctcgcattt attgcaacaa tatatttact tatatttaac tataaaacac tatttcagtg
     1561 acattagttt ctactggaaa gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct
     1621 ttcagcagga tttgcatcag gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta
     1681 tccagcctac caaacccgca ctgattgtag gcatgataag acgcgcaagc gtcgcatcag
     1741 gcatctgcgc acaaatgcct tatccgccta aaaacattaa acccgcaata acgcgcttcc
     1801 gagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg
     1861 tttaacgtat tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt
     1921 ttccagatcg tgttggtagc agacaaacag caaccccata tccagttgtc cggagttggt
     1981 gacccccaac gaataactgt agccgcgacg cagcatcagg ctggactcac tctccgccgt
     2041 gcggggattc gccagccgga tatggctgtc cagcgcaatc accttccctt ccgggtcgct
     2101 ggcgtaatca ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc
     2161 acggccaaaa atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa
     2221 ctgaatcaag cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc
     2281 tgccgttacc cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt
     2341 gccgtctttg aaacccagca aattaatcgg cgtctctttg cctttactac gcgacacgtg
     2401 atcggaaata aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat
     2461 gatatcgcgc agcgcatgga taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac
     2521 atcaccatga cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag
     2581 cttttttggc atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga
     2641 taacgtgatg gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg
     2701 tggcaggcgc ggatttggcg tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg
     2761 ctgagtcaac aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa
     2821 cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata
     2881 aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt
     2941 ttgtgcatga gcgaccggac aacttcccgc cagcgccagc gcaccaatcc ctttcagtaa
     3001 acgtcggcgt gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc
     3061 agtcccagca caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc
     3121 agtgcattcc ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg
     3181 tatttcgcca gaatggtatc gaccttttta aagttggcat cgacttttgc cagcagttcc
     3241 gggttggctt tttgcagttg tggacgcagc aaatcgacaa ttttctgcga gccttcaacg
     3301 ttagcctgga aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctaattttg
     3361 ctggctgcca cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa
     3421 gccagttcac tgatgcgttt ttgcaaatcg accacatctg tataaagctg ctcagcgtac
     3481 tgatccatcc ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca
     3541 gtgaattttg ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca
     3601 tccagatcgg agaacagttc agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc
     3661 gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg
     3721 gtgtcggtca ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca
     3781 ccaccaagac ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg
     3841 atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt
     3901 aaattcgccg tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc
     3961 atcacgcctt tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac
     4021 tgtgttttcc cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc
     4081 actttgacct gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc
     4141 acgctcaact gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa
     4201 agtatgttgt aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc
     4261 gcccctgcgc gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag
     4321 acggcgactt cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg
     4381 ccaaacagcg agtgagttga gagcactgcg ctcatatcga aggcgatttc ctgaaagtgg
     4441 ttccacaatc cggcttcatg aaatgcgcga atggcaccag cagccagccc tgcggcgacg
     4501 aagagaataa acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc
     4561 cagtagagca ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc
     4621 ggccagatcc cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct
     4681 tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc
     4741 tgcaatgcgc tatcgactgc ctgctccagt tgtactttga cgttgcgcga cactttgcgc
     4801 atccagaaaa ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac
     4861 agttcctgtt ctttttgcgg aaattcgccg gtggtttcgt taatgaagat acccaagccc
     4921 aggcacaacg cagcggcaag caacacgcca atccacatca cgccaatcca tcggcctcgc
     4981 tgggtacgct taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg
     5041 cgcaacataa tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca
     5101 atccatgcaa agaaaagtaa atcgcagtga tagcgattat cattacggca aagaaaaata
     5161 caagcggaat gtttataaaa tgatgttgga atgtaaacaa agagaggtaa taccagggga
     5221 tataaacgat tattccaggt acaaagatgg gattgtttac aacgagccat gtgttcttgc
     5281 cagatgccgc gtaaacgcct tatccagccg acaaaactgt ccagactcaa tgcatcacag
     5341 cttccatgta aaactgataa gcgggtgtat caagcaattt cgtccatccg cgattatcaa
     5401 atctaacttt ttgcccggaa cataataaca gcgcgggtgt tggtggcgtc aaaatcgctg
     5461 aaacgttccc cgaaggccgg ggcagcccac atggatgtgg gctgagggcg cgtcttacag
     5521 gacgttacct cgcgcccgac ccggtagc
//
LOCUS       DAZPLM010000246         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPLM010000246 DAZPLM010000000
VERSION     DAZPLM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.247"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHVC7_003876"
     CDS             complement(<1..1369)
                     /locus_tag="ACHVC7_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR2117792.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHVC7_003877"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHVC7_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR2117793.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHVC7_003878"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHVC7_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR2117794.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHVC7_003879"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHVC7_003879"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR2117795.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHVC7_003880"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHVC7_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR2117796.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPLM010000247         5489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPLM010000247 DAZPLM010000000
VERSION     DAZPLM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.248"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHVC7_003881"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHVC7_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR2117797.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHVC7_003882"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHVC7_003882"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR2117798.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHVC7_003883"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHVC7_003883"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR2117799.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHVC7_003884"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHVC7_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR2117800.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1357 a         1314 c         1489 g         1329 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaac
//
LOCUS       DAZPLM010000248         5454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPLM010000248 DAZPLM010000000
VERSION     DAZPLM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.249"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHVC7_003885"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHVC7_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR2117801.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHVC7_003886"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHVC7_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR2117802.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHVC7_003887"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHVC7_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR2117803.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHVC7_003888"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHVC7_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR2117804.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHVC7_003889"
     CDS             complement(2270..2761)
                     /locus_tag="ACHVC7_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR2117805.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHVC7_003890"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHVC7_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR2117806.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHVC7_003891"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHVC7_003891"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR2117807.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHVC7_003892"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHVC7_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR2117808.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1481 a         1353 c         1267 g         1353 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacg
//
LOCUS       DAZPLM010000249         5432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPLM010000249 DAZPLM010000000
VERSION     DAZPLM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.250"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            455..541
                     /gene="leuL"
                     /locus_tag="ACHVC7_003893"
     CDS             455..541
                     /gene="leuL"
                     /locus_tag="ACHVC7_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR2117809.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            634..2205
                     /gene="leuA"
                     /locus_tag="ACHVC7_003894"
     CDS             634..2205
                     /gene="leuA"
                     /locus_tag="ACHVC7_003894"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR2117810.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2205..3296
                     /gene="leuB"
                     /locus_tag="ACHVC7_003895"
     CDS             2205..3296
                     /gene="leuB"
                     /locus_tag="ACHVC7_003895"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR2117811.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3299..4699
                     /gene="leuC"
                     /locus_tag="ACHVC7_003896"
     CDS             3299..4699
                     /gene="leuC"
                     /locus_tag="ACHVC7_003896"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR2117812.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4710..5315
                     /gene="leuD"
                     /locus_tag="ACHVC7_003897"
     CDS             4710..5315
                     /gene="leuD"
                     /locus_tag="ACHVC7_003897"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR2117813.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5364..>5432)
                     /locus_tag="ACHVC7_003898"
     CDS             complement(5364..>5432)
                     /locus_tag="ACHVC7_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117814.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1404 a         1423 c         1409 g         1196 t
ORIGIN      
        1 tttgttgcga aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga
       61 ttaaatgtgc ttatttaata attaatttat atatttaatg cattaattct taacattaat
      121 tgatcaataa tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac
      181 aaatcatcag gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag
      241 atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa
      301 ttaatatcgc ttactttaac caccgcagca caattagcta attttacgga tgcagaactc
      361 acgctggcgg gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc
      421 taaaagcata tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg
      481 tctactacta ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta
      541 agccagcacg cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag
      601 cgaggcgctc taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat
      661 accacattgc gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg
      721 caaattgcgc tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc
      781 tcttcgccgg gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc
      841 gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa
      901 gtcgccgaag ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc
      961 aagctgcgca gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc
     1021 cgtaattaca ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc
     1081 gatctggcgc gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg
     1141 gacaccgtgg gctacaccat gccgcttgag ttcgccggaa tcatcagcgg cctgtatgaa
     1201 cgcgtaccta acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg
     1261 gcggtcggca actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg
     1321 aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa
     1381 gttcgtaagg atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc
     1441 agccagttag ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc
     1501 agcagcgcat tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa
     1561 aactacgaaa tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc
     1621 tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt
     1681 gaatataatt tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag
     1741 gtgtttgatt acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag
     1801 catttccgtc tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc
     1861 gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc
     1921 gatgccgtct atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac
     1981 agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac
     2041 tacaacggtc gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc
     2101 aaagccatgg tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg
     2161 caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc
     2221 atattgccgt attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag
     2281 tgctgaatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag
     2341 gcggcgcagc cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg
     2401 agcaagccga tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac
     2461 cagaccagca accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca
     2521 gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag
     2581 acattgccgc taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt
     2641 tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg
     2701 tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac
     2761 gtcgccacaa agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc
     2821 gtgaaatcgt caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca
     2881 tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct
     2941 ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga
     3001 tgttgccttc cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct
     3061 cagcaccaga tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg
     3121 cactgctgct gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca
     3181 ttaaccgcgc attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg
     3241 ttagtaccga tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat
     3301 ggctaaaacg ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga
     3361 aaccccgctg ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt
     3421 cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat
     3481 ggatcacaac gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat
     3541 ccagatgcag gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa
     3601 tcacccgtat caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg
     3661 gatgaccatt gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc
     3721 ctttggtatc ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg
     3781 ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa
     3841 agatatcgtg ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt
     3901 ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa
     3961 tatggcaatc gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa
     4021 ctatgtcaaa ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta
     4081 ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc
     4141 agaagaaatt tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa
     4201 cgacaatatt cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa
     4261 agcgctggcc tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa
     4321 agtgtttatc ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat
     4381 cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc
     4441 ggtaaaagcc caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga
     4501 atggcgcttg cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg
     4561 cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg
     4621 cacgcatctg gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga
     4681 cattcgcaac attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc
     4741 tggtggttcc gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt
     4801 tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg
     4861 atgaaaaagg ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg
     4921 cttccatttt gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct
     4981 gggcattgac cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct
     5041 acggcaatag ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg
     5101 aactgtttgc gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc
     5161 aagaggtgaa agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact
     5221 gcatgatgaa cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg
     5281 cttatgaagc aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg
     5341 gctttccgct atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg
     5401 acaacagaaa taaccgcaat tacccagtag ac
//
LOCUS       DAZPLM010000250         5337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPLM010000250 DAZPLM010000000
VERSION     DAZPLM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.252"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHVC7_003899"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHVC7_003899"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR2117815.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHVC7_003900"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHVC7_003900"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR2117816.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHVC7_003901"
     CDS             complement(2698..3390)
                     /locus_tag="ACHVC7_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR2117817.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHVC7_003902"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHVC7_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR2117818.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1265 g         1326 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggc
//
LOCUS       DAZPLM010000251         5194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPLM010000251 DAZPLM010000000
VERSION     DAZPLM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.253"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..387
                     /locus_tag="ACHVC7_003903"
     CDS             <1..387
                     /locus_tag="ACHVC7_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117819.1"
                     /translation="KSDIRQISNECQSEITKQAINTFSGIKQNFDQIASDHKKQLEIL
                     TKSAKPFSIKTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRFYARAFLDMKKITE
                     ESMRKLPKSDQQNIKLKLDEIDSRKP"
     gene            407..529
                     /locus_tag="ACHVC7_003904"
                     /pseudo
     CDS             407..529
                     /locus_tag="ACHVC7_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            861..1124
                     /locus_tag="ACHVC7_003905"
     CDS             861..1124
                     /locus_tag="ACHVC7_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117820.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            1135..1455
                     /locus_tag="ACHVC7_003906"
     CDS             1135..1455
                     /locus_tag="ACHVC7_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117821.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(1586..2023)
                     /locus_tag="ACHVC7_003907"
     CDS             complement(1586..2023)
                     /locus_tag="ACHVC7_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR2117822.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(2048..2230)
                     /locus_tag="ACHVC7_003908"
     CDS             complement(2048..2230)
                     /locus_tag="ACHVC7_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR2117823.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            2527..2970
                     /locus_tag="ACHVC7_003909"
     CDS             2527..2970
                     /locus_tag="ACHVC7_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117824.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(3358..3636)
                     /locus_tag="ACHVC7_003910"
     CDS             complement(3358..3636)
                     /locus_tag="ACHVC7_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR2117825.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
     gene            complement(4186..4437)
                     /locus_tag="ACHVC7_003911"
     CDS             complement(4186..4437)
                     /locus_tag="ACHVC7_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2117826.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
     gene            complement(4427..4687)
                     /locus_tag="ACHVC7_003912"
     CDS             complement(4427..4687)
                     /locus_tag="ACHVC7_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117827.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMSEGKNAK"
     gene            complement(4739..4963)
                     /locus_tag="ACHVC7_003913"
     CDS             complement(4739..4963)
                     /locus_tag="ACHVC7_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117828.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
BASE COUNT         1609 a         1136 c         1009 g         1440 t
ORIGIN      
        1 aaaagcgata taagacaaat atctaatgag tgtcagagcg agataaccaa acaagcaata
       61 aatacttttt ctggaatcaa acaaaatttt gatcaaattg cttccgacca taaaaaacaa
      121 ctagagatac taactaaatc agcaaaacca ttcagtatca aaacagccat tgcaatttgt
      181 gccctgtgca cattatgcct gtctgcggca ttttctggcg caacctggta tgttgcacaa
      241 catgaaaaag aggcaagtct gcgcttctat gcaagagcat ttcttgatat gaaaaaaata
      301 actgaagaga gcatgagaaa attaccaaaa tcagaccagc aaaatataaa actcaaacta
      361 gacgaaattg attcaagaaa accataaata tgaaaaggag tccctaatga ccacttttac
      421 cgacgaggac aaagaactaa ttaaagaaat cagagagcga attggcagcc tggacgtgcg
      481 agacaatatt gagcgtcggg cttatgaaat tgctctggca tcgctggaag cagaggcagt
      541 tatgttctgt atatcaggac aaaatgtaga ttcagaagaa catgtatcaa ccagcaaagc
      601 ggttgttgat gcctgggttg aagaatggaa tcaggttgac ggaagtcctg gcgaaccact
      661 gtacaaaact atgccactct actatcacgc tgccttgcca gcgccggtag tgcctgaagg
      721 actggttaaa gcggtgcgtt tttatgagca ggtaaaaaat gagaatccgg cagtcgaaac
      781 cgtagcatgg aaagacgctg ttgactgggt tctcgaagag gcttgtcagg ctgtaaacat
      841 tggcatcaaa ggagagtgat atggcaactt taacaaaaaa agaacgggca tggttgaacg
      901 aattacagga cgttcttgat cgctgcccat caccgaaaaa aattggtttt tacaccattg
      961 gcgataaaag catttacctg tatgacctgc gccgcatgga tgaaatcatg gaggctcttg
     1021 ataatcgttc gtcgatggat tggtgtgttg ctgtccatga tatgaatgcc ggatttgatg
     1081 aaaagatttt attcccctca tcagttgaaa gcacagcagg ataaggacta acacatgaca
     1141 acgttcaccg acaaagaact gattaaagaa atcaaagagc gtataggcag cctggacgtc
     1201 cgagacaata ttgagcgtcg ggcttatgaa attgctctgg catcactgga agcagaagcg
     1261 ccaaatttgc ctggtggttt cacaattgaa gaagcaaaag aattacacga aaatctggta
     1321 aaaagtcacg taagcaaggc attaagtggc gaaaaaatga aaaaagaaaa tcgtgatgcc
     1381 gatttacgct ggattcatgg cgtaatagtt caggctgcgt ggtttgtgaa agcatcacta
     1441 gggcagaacg catagtgcgt acctgctttt ctcctggcaa cggtggatct tccgcttcct
     1501 cgtcgctcgc tcgctatgct cggtcgctgg actgcggcga gcggttagct atcaaaaaaa
     1561 atccccgcac aacgcgggga tagttttatt tatatgcaag ttcatgacgt gccgcctgag
     1621 ccagaaaatg gctacgatct ttgtatacgc caccactttc tctcacatat ccatcaatac
     1681 gacgaatcaa tacatcaggc aatgaaatat taatacgttg ttgtttgccc tcaatttctg
     1741 acaaatcaac atcaattaca aaccagctat cacagtggtt ataatttggg ttggcggaat
     1801 aagtcaaaca cccttcatca tgaatatcat caacagaatg agcaccgctt attaccatct
     1861 cttccacgat tgccaatatc gcttcacgcg ccattgcggg tatttctgcc tgagaatcag
     1921 cagcagaaac acatccaaaa tcaaagcaat caaaaaccgg aacggtgata ccgtatgctg
     1981 tatgatcatc tttcggtgtt tcgacaccaa cagaaaagaa catggctacc tccaaagtcg
     2041 gcggggatta aatccccgcc atttttttga tcgatctgac agtacctagc ggcaagtcct
     2101 ttttaggatg aggaacagga aaagtttttc ctgttattgg cgaccaccat atctggtgac
     2161 tgcttgcctt atgccgtttc aactcgcaac cagcagctat cagttctttc aacaggtcag
     2221 cagatttcat atgtcctccc taacctgagg ataattatac acacacatac acacaaaggc
     2281 aaattatttc attcgtatga cttatgaacc acactgtaaa gacagctcaa tctttacagt
     2341 gtaagtcatt tatccagttt tatcgctgtg tatgtgatac atagaaaata atatagtatt
     2401 ttactatatg atatctgatt gacactttta actgcacatc tcatcattta catcgtaaag
     2461 acaaacatag atttacaatg taaagctatg taattaaaca ctccgtgtcc caaataagga
     2521 aagaaaatga ctcaactaat cccagaaaag atactggaaa tcatagatga tcatgatcgt
     2581 gcagaaaaaa agcaacgcaa caaaatcggt ttcatttatt tatgtctctg tttagcaata
     2641 attggtgtgg ccgcgtatac ctttatatca acgtttagca ttaatgtggt aacaatcatc
     2701 ataatgcttt tcggtgtgtt tatgattgtg atttttaaga gctatttaga ctcatggagt
     2761 gggataattc ttagcagtga ccacatattg tccatactcg ataaaaccaa agattaccca
     2821 gaaattaaac gtatagttat taatcgtctg ctctccggct caatactcac gggtaaagat
     2881 gaagagtaca tatacagcca actggaaaag gcccaacaaa gcaacgaacg tgaaaagaga
     2941 ttgcaagcaa ttaaagagta tacatcgtaa tacaacgcat tacgtcgaaa taccctggat
     3001 catcgaagcc acatacccct aagcgcagcg caaccgccgg aaaacgcacc acactgtaaa
     3061 gacaataaaa tctttacaac gtaaagctaa ctatgtaata caacgcgcta cacaataaga
     3121 aagggagagc atgacactct ccccatatat gactcaaact aaagcattat cccggttatg
     3181 cgcccaccaa atgatttcgc taataatcca tgcttctatc gcgggtgttt ttacaggttc
     3241 cccatttaca atgcggtttg caagctctat catttcacat actgacattt gagaatctcc
     3301 aaagatgcat ctgtcatttg gtaacaggct gccccacagc ctgttaccaa ccccaaatta
     3361 atatttgctg attactttac cctccttaat catttttatt agttcctgtt cagaatattc
     3421 ttttccgtgt tccaatgtgg aatatatatt gccaatagtc cataccccac actttttaat
     3481 ttctgcattt agccaggatt catgcagaat aaacaacggt tcgcaatcat ttcctttttc
     3541 ataaacataa atagattcgg tatcatttcc agaatcagaa gtaccaacga ctttttctat
     3601 atcgaaatgt ttttttagaa aagttgtgaa atgcattttc atgctcctgc cgctgagcgg
     3661 ctttggtggt tggggaccat cccctcccga tgtgtttata ataggtcaga actgacctat
     3721 tatcaagtat ttttccctcc attcttcaaa aaaaacacca tcaaatacag acagttacat
     3781 ccatgtgatg cccgacaggg catggaaggg gcgaaagaaa ataccaggga gcagcggggc
     3841 cacatcgccc cgaagcgcgg cgcagccgct gacgaacata ccaggatgca cagggccatc
     3901 ccttcgccct gcaaagccag aacagaccaa caggccatga tcatgctgat ttgcgccgca
     3961 gaatgccgca gttagcgcga gcgacagcga gtttacgccg tcctggtgca ttgcagcagg
     4021 gcggggcgct gtactgcgcg tgtgagcgtc agcgagctac gggcgcaatt gctcattgtt
     4081 caatgcaact ggggctatta agtccagcca taaattcagt tatggttttt gtagcttatt
     4141 ccgtgatttc gtcatacgtt aattctattt tcatcttaat attccttaat tacttccaga
     4201 atcagaattc tttttattcc ttttacaaag tacataatca gggtgttctc cagctaaaag
     4261 cagaagaaat ttaaactcta ctggtgtaag tgctgtaccg cttgcagttc cgacaggttc
     4321 ttctttgcga cgccaggtac gcggactcat gctgaatatt tcggcacatt ctttctgtgt
     4381 caggccaatt ttttgccgca gacgtttaat gttttctggt gtgttttcat ttggcatttt
     4441 ttccctcact catttcctga atcatcatag aaacaatttc tttatcattc tctcttaatc
     4501 cgtcgctggc aaagtcgata gtgtcgaatg ccttctcatg aaaaacaaga tcggcaacag
     4561 ttttcaccgt tgctttcgtt tttcctaaag tcgcagactt agaaaaagtt tttactcgct
     4621 tcaccagatg aaacgtatca ccttctttat tcagataata ttgtctgtca ccttttctta
     4681 tcttcatatc aagatagtct ccccttttaa cagtaaaggg gcatatagcc cctttctttt
     4741 aactttttcc ggtaatcaaa tcgacatctt cctgataaag ctccccaaga accggaacaa
     4801 cgcccttttt ataatcacgg atattatggc agtctgtaaa aatgacgaca taagcctcgc
     4861 ctgttttttc atgtttaagg caaattgtgc aaagcgtcac gcgcacaaca gaaaacacat
     4921 gaccagttgt agagacgata acaggaaaag tagattgttt catatttaat ctcctgccgc
     4981 tgagcggctt tggcgattgg ggaccatccc ctctcaatgc attaataata ggtcataaat
     5041 gacctattat caagtatttt ctaaacgagc tttattgaaa aatcattttc aataaattca
     5101 tctaacatct ttaatatacc agctttattt acgaaatcat cctccataat ttcgatactt
     5161 gctgatattt cacatgtaaa ctgaatgctc ctgt
//
LOCUS       DAZPLM010000252         5194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPLM010000252 DAZPLM010000000
VERSION     DAZPLM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.254"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHVC7_003914"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHVC7_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR2117829.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHVC7_003915"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHVC7_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR2117830.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHVC7_003916"
     CDS             1797..1970
                     /locus_tag="ACHVC7_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117831.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHVC7_003917"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHVC7_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR2117832.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHVC7_003918"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHVC7_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR2117833.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHVC7_003919"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHVC7_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR2117834.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHVC7_003920"
     CDS             complement(4268..5191)
                     /locus_tag="ACHVC7_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2117835.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         1341 a         1208 c         1306 g         1339 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taag
//
LOCUS       DAZPLM010000253         5193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPLM010000253 DAZPLM010000000
VERSION     DAZPLM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.255"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHVC7_003921"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHVC7_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR2117836.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHVC7_003922"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHVC7_003922"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR2117837.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHVC7_003923"
     CDS             2475..4124
                     /locus_tag="ACHVC7_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR2117838.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5193)
                     /locus_tag="ACHVC7_003924"
                     /pseudo
     CDS             complement(4278..5193)
                     /locus_tag="ACHVC7_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1127 a         1206 c         1398 g         1462 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac caaggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taa
//
LOCUS       DAZPLM010000254         5084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPLM010000254 DAZPLM010000000
VERSION     DAZPLM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.256"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHVC7_003925"
     CDS             <1..488
                     /locus_tag="ACHVC7_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2117839.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="ACHVC7_003926"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="ACHVC7_003926"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR2117840.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHVC7_003927"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHVC7_003927"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR2117841.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHVC7_003928"
     CDS             complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHVC7_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR2117842.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHVC7_003929"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHVC7_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR2117843.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHVC7_003930"
     CDS             complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHVC7_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR2117844.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4693..>5084
                     /gene="dgcM"
                     /locus_tag="ACHVC7_003931"
     CDS             4693..>5084
                     /gene="dgcM"
                     /locus_tag="ACHVC7_003931"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR2117845.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGF"
BASE COUNT         1382 a         1297 c         1210 g         1195 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt ttta
//
LOCUS       DAZPLM010000255         4996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPLM010000255 DAZPLM010000000
VERSION     DAZPLM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.257"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            295..1269
                     /gene="hemB"
                     /locus_tag="ACHVC7_003932"
     CDS             295..1269
                     /gene="hemB"
                     /locus_tag="ACHVC7_003932"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR2117846.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1375..2226)
                     /gene="tauD"
                     /locus_tag="ACHVC7_003933"
     CDS             complement(1375..2226)
                     /gene="tauD"
                     /locus_tag="ACHVC7_003933"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR2117847.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2223..3050)
                     /gene="tauC"
                     /locus_tag="ACHVC7_003934"
     CDS             complement(2223..3050)
                     /gene="tauC"
                     /locus_tag="ACHVC7_003934"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR2117848.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3047..3814)
                     /gene="tauB"
                     /locus_tag="ACHVC7_003935"
     CDS             complement(3047..3814)
                     /gene="tauB"
                     /locus_tag="ACHVC7_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR2117849.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3827..4789)
                     /gene="tauA"
                     /locus_tag="ACHVC7_003936"
     CDS             complement(3827..4789)
                     /gene="tauA"
                     /locus_tag="ACHVC7_003936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2117850.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1194 a         1426 c         1249 g         1127 t
ORIGIN      
        1 cgttataaat atcaaaaaca caaaccagat aaatatctta tcaatataca acaaagtttg
       61 aattattcaa tttatagcag cacaaccaaa cgccaaaaaa aataataacc aagagctata
      121 aataaagatt aaaaatattt tcagtgaata tgaaattatt gctatttcta ttacaaagat
      181 gtcctggtct ctctttcatt ctcttcttgt caacatcgcg acaactttcg taaaacactc
      241 ctgccccgct tcaggtatac tgtgcacctt aattccacaa acatcaggca gaccatgaca
      301 gacttaatcc aacgccctcg tcgcctacgc aaatctcctg cgctgcgcgc tatgtttgaa
      361 gagacaacac ttagccttaa cgacctggtg ttgccgatct ttgttgaaga agaaattgac
      421 gactacaaag ccgttgaagc catgccaggc gtgatgcgca ttccagagaa acatctggca
      481 cgcgaaattg aacgcatcgc caacgccggt atccgttccg taatgacttt tggcatctct
      541 caccataccg atgaaaccgg cagcgacgcc tggcgggaag atggactggt ggcgcgtatg
      601 tcgcgcatct gcaagcagac tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt
      661 gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg
      721 actctggaaa atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc
      781 gccccttccg ccgcgatgga cggccaggta caggcgattc gtcaggcgct ggacgctgca
      841 ggctttaaag atacggcgat tatgtcgtat tcgaccaagt tcgcctcttc cttttatggc
      901 ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac
      961 ccaatgaacc gtcgcgaggc gattcgtgaa tcactgctgg atgaagccca gggcgcagac
     1021 tgcctgatgg ttaaacctgc tggagcgtac ctcgacatcg tgcgtgagct gcgtgaacgt
     1081 actgaattgc cgattggcgc gtatcaggtg agcggtgagt acgcgatgat taagttcgcc
     1141 gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag
     1201 cgtgcgggtg cggatctgat tttcagctac tttgcgctgg atttggctga gaagaagatt
     1261 ctgcgttaat ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg
     1321 ccccctcacc ctaaccctct ccccaagggg gcgagggaac cgtccgcact cgtatcaccc
     1381 cgcccgataa aacggtttat ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg
     1441 caggtaatcg gcattggcat agtgctgagt cacgcggtta tcccaaatcg caatatcatt
     1501 cggttgccag cgccaacgca cctgaaactc cggtttagtg atatgggcga ataaaaagcc
     1561 taacaaggct tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa agccttcatt
     1621 cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc accaccggat gtagcaacgg
     1681 aggatttttc gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt
     1741 gtattccggg aacgatttac ggaaatcatg ctccgcacgc agcccgctca gcagctggcg
     1801 gaagggaaca gagagcgcct cataggccgc aataccgctg gtccagagcg tatcaccgcc
     1861 ggtcgaaggt aactctttag ctgccagaat cgcccctgcg ggtggcgttt caataaatgt
     1921 cacatcggta tgccagttgt cgttatccgg cggattatcg ttatgggtat ccagcacgat
     1981 gatctcgtca accccttcgg catgagggta aacagggtga atatgcagtt cgccaaaacg
     2041 ctgggccagc gcgcgttgct gctgcggcgt aatagcttga tcgcgcagaa ataccacctg
     2101 atagcgcagc accgcatggt aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag
     2161 gtcggcaccc gaaatttgtg cgccgatata cggccccagc ggggtaatac tcagacgttc
     2221 actcattgta cttctccatg ccagggcgtc agacggcgct gtaacgcgcg cagacccagt
     2281 tctaaaagaa aggcgataat cgcaatcacc gcgatccccg ccagtaccac gtcagtcgcg
     2341 agaaattcac ccgctgactg aaccataaat cctaaaccgc gtgtcgcggc aatcagctcc
     2401 gccgctacca gcgtagacca gcccacaccc agaccaatgc gtaatccggt gaggatttcc
     2461 ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg cacggctggc acccagcgac
     2521 tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcac cgacatcgcc
     2581 accggtgcaa aaatcgctaa atagatcaat aagatcttcg aggtttcacc aataccaaac
     2641 cagatcacca tcagcggcaa ataagccagc ggcggcaccg gacgataaag ttcgattacc
     2701 ggatccagaa tgccgcgcac cgtcgggctt agccccatgg cgatcccgac cggaataccg
     2761 atcaccaccg ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc cagatgctgc
     2821 cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg tgagcagttt tgccagtact
     2881 tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca gcgccgccac cgtccaccat
     2941 acagtgagta aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga gagcggccag
     3001 cgccatttca gccgccgcga atgcagtttt tcattaatga gcacactcat gagaacgcct
     3061 cccgttgctc aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg
     3121 acttgatgct gcggctcgac tctcccgcaa caaagcggcg agcaaagttg agcggcagcc
     3181 gctccagcac acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg
     3241 cttcttctat atcgtgggta atcaacagca cctgcttgcc cgtctcctgc cagagtttca
     3301 gcagcagagt ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga
     3361 gtaataacag ctggggattc gccgccagcg cgcgggcaat ccccacccgc tgacgttgac
     3421 caccggaaag ctgccagata tagcgttttt ctgcgccttc cagccccact tttttcagca
     3481 tctggtgcgc gatttccagt cgctgcattt tctctatacc tgccaattgc aggccgaacg
     3541 ccacgttgtc ctgtacattg cgccacggta gtagcccttc attctgaaaa accaccccgc
     3601 gctctgctcc cggtccctca atacgcttac ccgccagttg aatgctgcca tgctgataag
     3661 gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc gcagccggac ggccccagca
     3721 ccaccagtag ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc
     3781 cataatcggc atagagatga gagatttgca gcatctgcgc ctccttttat tgcacgaagc
     3841 gcgaggtaac gtactggctg taatcattcg ctacagccgg gaccttgccc tgctctttca
     3901 aaaactgcgc ggtgtcgatg attgctttgt tcaccggtcc ggtcagttct gccgtttgtt
     3961 gctgcggcgt cagataggta ttccccttca ccagccccgg aacgtcacct tccggcacgc
     4021 cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt cagccacgcg tctgggttag
     4081 caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg acctcaggat
     4141 gtttctcggc aaaatctttg cgcaccaccc agacgtccag cgttggcgca ccccactgcc
     4201 cgacctgttc agaatcggtc aacaccttgc cgtctttttc cagggcgtta accgccggtg
     4261 cccagacata agcaccatca atatcacccc gctgccatgc ggcgataatc gcgggcggct
     4321 gcaggttcac aatctccact tgccctggtt taatccccca gtgtttcagc gccgccagca
     4381 ggctgtagtg ggtggtggag ataaacggta cggcaatgcg tttgccaatc aaatcttcgg
     4441 gtttgctgat agttttcttt accaccagcg cttcggagtt acccagtttt gacgccagca
     4501 agaagacttc aatcggcacc tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt
     4561 tgccgatttg cacgtcgcca gaagccagcg cccgcacgat gctggctccg ctgtcaaact
     4621 tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt gttgtcggcc tgagccactt
     4681 tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag
     4741 cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga aattgccatg attgtctgct
     4801 cccctgtctt gttatgggag cagtattcag gaataaaaac attcattaaa agaattagtc
     4861 gttatcgcac agatgatttt attcttagca aaaaaacggc aaaggaaaga ttaaatatgc
     4921 aattactccg ttctaacaat tgggaaaatt actacacttc attttagtga tgcataaaca
     4981 accacattac gagatt
//
LOCUS       DAZPLM010000256         4892 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPLM010000256 DAZPLM010000000
VERSION     DAZPLM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.258"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            219..776
                     /gene="pinE"
                     /locus_tag="ACHVC7_003937"
                     /pseudo
     CDS             219..776
                     /gene="pinE"
                     /locus_tag="ACHVC7_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1115..1963)
                     /locus_tag="ACHVC7_003938"
     CDS             complement(1115..1963)
                     /locus_tag="ACHVC7_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR2117851.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2172..2807
                     /gene="yfhb"
                     /locus_tag="ACHVC7_003939"
     CDS             2172..2807
                     /gene="yfhb"
                     /locus_tag="ACHVC7_003939"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR2117852.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2865..3368
                     /gene="tadA"
                     /locus_tag="ACHVC7_003940"
     CDS             2865..3368
                     /gene="tadA"
                     /locus_tag="ACHVC7_003940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR2117853.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(3365..4891)
                     /gene="mltF"
                     /locus_tag="ACHVC7_003941"
     CDS             complement(3365..4891)
                     /gene="mltF"
                     /locus_tag="ACHVC7_003941"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR2117854.1"
                     /translation="MGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPL
                     TYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYN
                     SERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKDT
                     KFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQP
                     VTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVDTRTFLRAVDA
                     VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGIT
                     DRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTK
                     GNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEK
                     QATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
                     "
BASE COUNT         1185 a         1176 c         1255 g         1276 t
ORIGIN      
        1 tatttgttgc taataatcat gtataaaata agcttatata catagtaact tcagcaaagg
       61 atttagtaat gatccaaatc atatctcaat gtactgtttt tttctttaaa aaaacagtac
      121 attctacttt ataatgactg tgtattccca tggaaggaga tcacccaatt ctcccccccg
      181 acttttgacg cagtgtcttg ttaacagggg gatttaccat gcttattggc tatgtacgcg
      241 tatcaacaaa tgaccagaat accgatctac aacgtaatgc gttgaactgt gcaggatgcg
      301 agctgatttt tgaagacaag ataagtggca caaagtccga aaggccgggg ctgaaaaaac
      361 tgctcaggac attatcggca ggcgacacac tggttgtctg gaagctggat cggctggggc
      421 gcagtatgcg gcatcttgtc gtgctggtgg aggagttgcg cgaacgaggc atcaactttc
      481 gtagtctgac tgattcaatt gataccagca caccaatggg acgctttttc ttccatgtga
      541 tgggtgccct ggctgaaatg gaacgtgaac tgattgttga acgaacaaaa gcaggactgg
      601 aagctgctcg cgcacaggga cgaattggcg gtcgccgtcc caaactttca ccagaataat
      661 gggcgcaggc cgggcgacta attgcatcag gagtttctcg ccagaaggtg gcgatcatct
      721 atgatgttgg cgaatcgact tttttgtata agaggtttcc agccagggtt atataaaacc
      781 gcagacacgg cgtatacaag aacgtgctac ggttgactga aaagttaatg tggtatgtca
      841 caggcctttt gcgccgtaaa gccccgtcct ttagggcgcg gaggacgtta aagaaagtgg
      901 tgttctttgt gttgctgaac ttatgcaaaa tttttttgca ccataaatcc aacaacacat
      961 tcaataacac attgctgtat cactatgaaa ataaatgtgt ttttatttga tttcacacat
     1021 atcacaattg atgcattttt tagtgattaa caacgccata aggaaactct caaaacaaca
     1081 caaaccgggc gggcattgta cgcgcattct ttactcagac cagttttttc accagcgctt
     1141 cactatgacg aatacgttct ggtgccagtt ccagatcctg ctgaatcagc gcaatgaaca
     1201 gcaaatccgt taacattccc tgagcgtgac aagcagagat tgaagcactg tttgtcgctt
     1261 gttcttcggc aatggtatac aggcaatgag aagcacgctg ttgcagggca ttcggagtaa
     1321 agccggtaat cgccagcact tttccgccca ctcgcagcat ctcatctgcc gccaggttta
     1381 actcgcgtcg tacaccggtg taggaaatgg ctaataacag atcgtcaggg gacgacgcct
     1441 gtactgttgc gagtagcgca tgcatatcgc gcactgcggc agcattgaag ccaatcttca
     1501 tcagcttcca ggcaaagttt tgcgccacca gacccgaagc gccaataccg gtcagaatta
     1561 tccgccgcgc agagcgcaac attgttacgc attcatgcag tttctcttca ctattaacgt
     1621 ttagcgttgc gtacatagcg gcagtatttt ctttaatcag tttttcgccg accagccgta
     1681 acggatcatc accgcggatt tggttatgaa tgggcacgga gggcgattcc ggctggcttg
     1741 ccagcgcttc actcaacgcc aacttaagcg ccggaaaacc tttatagccg agtttttgcg
     1801 cgaacttcac gacgctggac tgactgactc cggcttcgtt ggccagttgc tgagagctta
     1861 aatggcgcgc cgtatcaggt tgtagcagca gataatccgc cagtttttta tcactctggg
     1921 caagcccctg gtaacgctga cggatacgaa gtaagccgtt catatctttc ctcgcgatga
     1981 aatagtggcg acaacaggca atatgagaca cacgcgagtg aattttatat tccattgccg
     2041 ctaattgcca tgaattaaaa attccagtta gccatgatgc ggtcgattgt tgccggaaaa
     2101 gtgatgatct atctgttagg ctatgtagta gcgaaaatta atcaggatgt ttcagtccag
     2161 aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt ttttgactta gatggaacat
     2221 tgcatcagca ggatatgttc ggcagttttc tgcgctattt actgcgtcgc caaccgctga
     2281 atgcgttact tgtcctgccg ttgttaccga ttatagccat tgcgttattg ataaaaggtc
     2341 gtgcggcacg ctggccgatg agtctgcttc tgtgggggtg cacttttggt cacagcgaag
     2401 cacgtttaca gacgttgcag gccgatttcg tgcgctggtt tcgcgacaat gttaccgcct
     2461 ttccgctggt tcaggagcga ttaaccacct acctgttaag ttccgatgct gatatctggt
     2521 tgattaccgg ctctccgcag ccgctggttg aagcggttta tttcgatacg ccctggctgc
     2581 cgcgggttaa tcttatcgcc agccaaattc agcgtggcta tggtggttgg gtattgacga
     2641 tgcgttgtct gggacatgaa aaggtcgcac aactggagcg caaaatcggc actccgctgc
     2701 ggctgtacag tggctatagc gacagtaatc aggacaatcc gctgctttat ttctgtcagc
     2761 atcgttggcg agtaaccccg cgcggtgaac tccagcaact ggaatagagt aaagcatagc
     2821 gtccgtgtat aatgcgccgc gcttttataa ccggagtttt ctttttgtct gaagtcgaat
     2881 ttagccacga atactggatg cgtcacgcgc tgacgctggc gaaacgtgcc tgggatgagc
     2941 gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg ggtaatcggc gaaggctgga
     3001 accgcccgat tggtcgccat gatcccaccg cacatgcaga aatcatggcc ctgcggcagg
     3061 gtggtctggt gatgcaaaat tatcgtctga tcgacgccac gttgtatgtc acgcttgaac
     3121 catgtgtaat gtgtgccgga gcgatgatcc acagtcgcat tggtcgcgtg gtctttggtg
     3181 cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga tgtgctgcat catccgggta
     3241 tgaatcaccg agtggaaatt acggaaggaa tactggcgga tgagtgcgcg gcgttgctca
     3301 gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca gaaaaaagcg caaccctcga
     3361 cggattaatt ttgtttctct tcactccctt tcctggaaaa cagcagagag ggagaatggg
     3421 tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa aggaaatttc tctttgccca
     3481 actccgtagg cgataccgcc ggataatcct gcgccagttg catcgccgct tctgtagcct
     3541 gcttctcttt ctcttgcaga taacccacca ggctaatctg atacttacga atattttcga
     3601 cataagcgta ggcttcatgt ccacgagcgt agccgtaagt cagcttgctg taatagggtt
     3661 tctggctaag taaaggcaga cgctgtttta cgtcagccca actgtcagga ttccctttgg
     3721 tttttgccgt cagggcgcgg gcatccagca tatgcgcata gcccatattg tacgcagcga
     3781 gggcaaacca gatccgctcg ttctccggca cactttccgg cactttactc atcatatcct
     3841 gcaaataacg cacgccaccg ctgatgctct gttcggcatc ggtacgatcc gtaatgccga
     3901 ggctttgcgc ggtatttttg gttaacatca tcatgccgcg cacacccgtc ggtgaagtgg
     3961 cctgtgcatc ccagtgcgat tcctgataag caatagcggc cagcaaacgc cagtcaattt
     4021 cttcggcgta tttctcaaac aggggcttta actgcggcag taccgcatcg acggcgcgta
     4081 aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg ccccaggtat ttctcttcaa
     4141 tgcgtgccag cgtacagtct tcattcattt cgttgaagaa gtcgagcagg gcggcggaaa
     4201 gggtattatc gccatctaac gggctaaacc aagtcaccgg ttgttcatcg gtgatatcga
     4261 gcgctacggc gagctccggg tgaacgcgct gaaacaggct gatggcgaca gaatcagcaa
     4321 tggtgtaatc gagttttcct tcgatgacat cttccattaa ttccgcagag ccttttttgt
     4381 cgtctacctt ccagcttaat tccgggaatt ttgtgtcttt cagggtttgg agatcgttaa
     4441 ccaccacgtg acccggtgca acggtgagtt gctccgccgt caggttgccc agtgtgcgcg
     4501 ggcgatactg actcacttta taaaccagtt gttgtgacac ggaataatag gtagggccag
     4561 gctgataatt ttttacccgc tcactgttat agaccagtcc tgccgccagc aggtcggcgt
     4621 taccattatc gaggtcgtca aacagctggc tgatattctg ccgcacggtc actttcagtt
     4681 ttacaccgag gtaatcggca aactgtttcg ccagttcgta atccaggcca aaaggtttcc
     4741 cgttgatttc gttataagtc aggggagtat gaatggtgct cacacgcaac tctccccgcg
     4801 cttgaatggc ggcgatacgg ttgtcggctt taccaaacca gggaatggat ggccagagag
     4861 cgaccgcgag cagcagtgcc agaatgccga tg
//
LOCUS       DAZPLM010000257         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPLM010000257 DAZPLM010000000
VERSION     DAZPLM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.259"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHVC7_003942"
     CDS             complement(34..954)
                     /locus_tag="ACHVC7_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2117855.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHVC7_003943"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHVC7_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR2117856.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHVC7_003944"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHVC7_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR2117857.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHVC7_003945"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHVC7_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR2117858.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZPLM010000258         4829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPLM010000258 DAZPLM010000000
VERSION     DAZPLM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.260"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(103..546)
                     /locus_tag="ACHVC7_003946"
     CDS             complement(103..546)
                     /locus_tag="ACHVC7_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR2117859.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            703..1632
                     /gene="metA"
                     /locus_tag="ACHVC7_003947"
     CDS             703..1632
                     /gene="metA"
                     /locus_tag="ACHVC7_003947"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR2117860.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1901..3502
                     /gene="aceB"
                     /locus_tag="ACHVC7_003948"
                     /pseudo
     CDS             1901..3502
                     /gene="aceB"
                     /locus_tag="ACHVC7_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3532..4823
                     /gene="aceA"
                     /locus_tag="ACHVC7_003949"
                     /pseudo
     CDS             3532..4823
                     /gene="aceA"
                     /locus_tag="ACHVC7_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1203 a         1272 c         1223 g         1131 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcat tggcgcagaa aaaaatgccg ggtgcgacgc
       61 tggcgcgtct tctccagcct gggcaggcac ggcaggggca gcttattcct ccacatacgc
      121 cagattcagc aacggatacg gtttccccaa atcgtccacc tcagagcgtc ccgtaacctt
      181 aaaacccacc ttcttataga acccaaccgc ctgctcattt tgctcattaa cgttggttgt
      241 cagttccggt gccatcgaga gcgcatgctc caccagcatc cgacccacgc cgcagccgca
      301 cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca taaacccaac
      361 cggctgatcc cgttcattaa ccgcgaccca caacggcgct tccggcagga aggaacgaac
      421 tagcacctcc agctcggccc gatactctgc tgataaaaaa tcgtgtgtgg catcgacaga
      481 acgacaccaa atcgcaacga gttcctcccc ttcctcatgc cgtgagcggc gaatactaat
      541 aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc cttgaaactt
      601 tctcaccttc aacatgcagg ctcgacattg gcaaattttc tggttatctt cagctatctg
      661 gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat tcgtgtgccg
      721 gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat gacaacttct
      781 cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga tccttaacct gatgccgaag
      841 aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt gcaggtcgat
      901 attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga gcatctgaac
      961 aacttctact gtaactttga agatattcag gaacaaaact ttgacggtct gatagttacc
     1021 ggcgcgccgc tgggcctagt ggagtttaat gatgtcgctt actggccgca gatcaaacag
     1081 gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg ggcggtacag
     1141 gccgcgctca atatcctcta cggcattcct aagcaaactc gcaccgacaa actctctggc
     1201 gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt tgatgattca
     1261 ttcctggcac cgcattcgcg ctatgctgac tttccggcag cgttgattcg tgattacacc
     1321 gatctggaaa ttctggcaga gacggaagaa ggggatgcat atctgtttgc cagtaaagat
     1381 aagcgcattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct ggcgcaggaa
     1441 tatttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta tttcccgcac
     1501 aatgatccgc aaaacaaacc gcgcgcgagc tggcgtagtc acggtaattt gctgtttacc
     1561 aactggctca actattacgt ctaccagatc acgccatacg atctacggca catgaatcca
     1621 acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct tacctcaggc
     1681 accttcgggt gcctttttta tttccgaaac gcacctcagc aagtgaataa attttattca
     1741 tattgttatc aataagttat caagtatttt taattaaaat ggaaattgtt tttgattttg
     1801 cattttaaat gagtagtctt agttgtgctg aacgaaaagc gcacaacgat ccttcgttca
     1861 cagtggggaa gttttcggat ccatgacgag gagctgcacg atgactgaac aggcaacaac
     1921 aaccgatgaa ctggctttca caaggccgta tggcgagcag gagaagcaaa ttcttactgc
     1981 cgaagcggta gaatttctga ctgagctggt gacgcatttt acgccacaac gcaataaact
     2041 tctggcagcg cgcattcagc agcagcaaga tattgataac ggaacgttgc ctgattttat
     2101 ttcggaaaca gcttccattc gcgatgctga ttggaaaatt cgcgggattc ctgcggactt
     2161 agaagaccgc cgcgtagaga taactggccc ggtagagcgc aagatggtga tcaacgcgct
     2221 caacgccaat gtgaaagtct ttatggccga tttcgaagat tcactggccc cggactggaa
     2281 caaagtgatc gacgggcaaa ttaacctgcg tgatgcagtt aacggcacca tcagctatac
     2341 caatgaagca ggcaaaattt atcagctcaa gcccaatcca gcggttttga tttgtcgggt
     2401 acgcggtcta cacttgccgg aaaaacatgt cacctggcgt ggtgaggcaa tccccggtag
     2461 cctgtttgat tttgcgctct atttcttcca caactatcag gcactgttgg caaagggcag
     2521 cggtccctat ttctatctgc cgaaaaccca gtcctggcag gaagcggcct ggtggagcga
     2581 agtcttcagc tatgcagaag atcgctttaa tctgccgcgc ggcgccatca aggcgacgtt
     2641 gctgattgaa acgctgcccg ccgtgttcca gatggacgaa atccttcacg cgctgcgcga
     2701 ccatattgtt ggtctgaact gcggtcgctg ggattacatc ttcagctata tcaaaacgtt
     2761 gaaaaactat cccgatcgtg tcctgccaga cagacaggcc gtgacgatgg ataaaccatt
     2821 cctgaatgct tactcacgcc tgctgattaa aacctgccat aaacgcggtg cttttgcgat
     2881 gggcggcatg gcagcgttta ttccgagcaa agatgaagag cgtaataacc aggtgctcaa
     2941 caaagtaaaa gcggataaag cgctggaagt caataacggt cacgatggca catggatcgc
     3001 tcacccaggc cttgcggaca cggcaatggc ggtattcaac gacattctcg gctcccgtaa
     3061 aaatcagctt gaagtattgc gcgaacaaga cgcgccgatt actgccgatc agctgctggc
     3121 accttgtgac ggtgaacgca ccgaagaagg tatgcgcgcc aacattcgcg tggctgtgca
     3181 gtacatcgaa gcgtggatct ccggcaacgg ctgcgtgccg atttatggcc tgatggaaga
     3241 tgcggcgacg gctgaaattt cccgtacctc gatctggcag tggatccatc atcaaaaaac
     3301 gttgagcaat ggcaaaccgg tgaccaaagc cttgttccgc tagatgctgg gcgaagagat
     3361 gaaagtcatt gccagcgaac tgggcgaaga acgtttctcc cagggccgtt ttgacgatgc
     3421 cgcacgcttg atggaacaga tcaccacttc cgatgagtta attgatttcc tgaccctgcc
     3481 aggctaccgc ctgttagcgt aaaccaccac ataactatgg agcatctgca catgaaaacc
     3541 cgtacacaac aaattgaaga attacagaaa gagtggactc aaccgcgttg ggaaggcatt
     3601 actcgcccat acagtgcgga agatgtggtg aaattacgcg gttcagtcaa tcctgaatgc
     3661 acgctggcgc aactgggcgc agcgaaaatg tggcgtctgc tgcacggtga gtcgaaaaaa
     3721 ggctacatca acagcctcgg tgcactgact ggcggtcagg cgctgcaaca ggcgaaagcg
     3781 ggtattgaag cagtctatct gtcgggatgg caggtagcgg cggacgctaa cctggcggcc
     3841 agcatgtatc cggatcagtc gctctatccg gcaaactcgg tgccagctgt ggtggagcgg
     3901 atcaacaaca ccttccgtcg tgccgatcag atccaatggt ccgcgggcat tgagccgggc
     3961 gatccgcgct atgtcgatta cttcctgccg atcgttgccg atgcggaagc cggttttggc
     4021 ggtgtcctga atgcctttga actgatgaaa gcgatgattg aagccggtgc agcggcagtt
     4081 cacttcgaag atcagctggc gtcagtgaag aaatgcggtc acatgggcgg caaagttttg
     4141 gtgccaactc aagaagctat tcagaaactg gtcgcggcgc gtctggcagc tgacgtgacg
     4201 ggcgttccaa ccctactggt tgctcgtacc gatgccgatg cagcggatct gatcacctcc
     4261 gattgcgacc cgtatgacag cgaatttatt accggcgagc gtaccagtga aggcttcttc
     4321 cgtactcatg cgggcattga gcaagcgatc agccgtggcc tggcgtatgc gccatatgct
     4381 gacctggtct ggtgtgaaac ctccacgccg gatctggaac tggcgcgtcg ctttgcacaa
     4441 gctatccacg cgaaatatcc gggcaaactg ctggcttata actgctcgcc gtcgttcaac
     4501 tggcagaaaa acctcgacga caaaactatt gccagcttcc agcagcagct gtcggatatg
     4561 ggctacaagt tccagttcat caccctggca ggtatccaca gcatgtggtt caacatgttt
     4621 gacctggcaa acgcctatgc ccagggcgag ggtatgaagc actacgttga gaaagtgcag
     4681 cagccggaat ttgccgctat accttcgtat ctcaccagca ggaagtgggt acaggttact
     4741 tcgataaagt gacgactatt attcagggcg gcacgtcttc agtcaccgcg ctgaccggct
     4801 ccactgaaga atcgcagttc taagcaacc
//
LOCUS       DAZPLM010000259         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPLM010000259 DAZPLM010000000
VERSION     DAZPLM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.261"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHVC7_003950"
     tRNA            6..81
                     /locus_tag="ACHVC7_003950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHVC7_003951"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHVC7_003951"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHVC7_003952"
     CDS             complement(299..1057)
                     /locus_tag="ACHVC7_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR2117861.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHVC7_003953"
     CDS             complement(1065..2168)
                     /locus_tag="ACHVC7_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR2117862.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHVC7_003954"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHVC7_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHVC7_003955"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHVC7_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPLM010000260         4791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPLM010000260 DAZPLM010000000
VERSION     DAZPLM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.262"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(147..3368)
                     /gene="carB"
                     /locus_tag="ACHVC7_003956"
     CDS             complement(147..3368)
                     /gene="carB"
                     /locus_tag="ACHVC7_003956"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR2117863.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3386..4534)
                     /gene="carA"
                     /locus_tag="ACHVC7_003957"
     CDS             complement(3386..4534)
                     /gene="carA"
                     /locus_tag="ACHVC7_003957"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR2117864.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1049 a         1347 c         1238 g         1157 t
ORIGIN      
        1 gtgtgagcta aatcctgctt atgtcatcaa aaatatcgat tccccaaaag atttacttaa
       61 tgggcaaaaa aaacggtcct catcagagaa ccgtattact tattcgatca aattagcgga
      121 tgaaaaatat ccgccatgac acgctattat ttgatctgcg cgtgcatttc ctgcaccgaa
      181 attacttttt cggtcgcgtc ggcattcagc gccatcgcgg tagcgaaacc accgttcagg
      241 gtggtgtcat aatgcacttt atattgcagc gcactgcgac ggatcacgcg ggagtcttca
      301 atcgcgcgac ggcctgaagt ggtgttgatg atgtaggtat attcgccatt cttgatgcgg
      361 tcctgaatgt gcggacggcc ttcatgcacc ttgttcacca gacgcggatt aatgcccgct
      421 tcgcccagca caatcgccgt gccgtgggtc gcatccagct cgaagccctg tttcagcagt
      481 tttgccgcca ggtccaccac gcgttcttta tcgccttcgc gcacggaaag caacgcacga
      541 ccgtgtttct tcatggtgga gttgctgccc agctgcgctt tggcaaacgc ttcagcgaag
      601 gtgcggccca cgcccatcac ttccccggtg gagcgcattt ctggccctaa cagcgggtca
      661 acacccggga atttattgaa cggcagcacc acttctttca ccgagtagta cggcgggata
      721 acttctttgg ttacgccctg ctcagccagc gatttgccag ccatcacacg cgccgccact
      781 tttgccagcg gtacgccggt ggctttggag acgaacggaa cggtacgcgc cgcacgcggg
      841 ttaacttcaa tcaggtagac ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg
      901 cgcacctgca attcgaaggc cagtttctgc acctgctggc gcatcacatc ctgaatttcc
      961 tgacttaagg tgtaggctgg cagagaacat gcggagtcac cggagtgcac gcccgcctgc
     1021 tcgatgtgct ccatgatgcc gccaatcagc accatttcgc cgtcgcagat ggcatccacg
     1081 tcaacttcta ccgcgtcatc aaggaagtga tccagcaaca ctggcgcatc gttagacacg
     1141 ctgaccgccg tctggaagta gcgacgcagg tcagcttcgt catagacgat ttccatcgcc
     1201 cgaccgccga gaacgtaaga cggacgtacc accagtgggt agccaatctc tttcgccttc
     1261 tctaccgcca tttcaatagc ggtaacggtg gcgttcgccg gttgtttcag tttcagacgc
     1321 tcaaccgcat gctggaagcg ttcacggtct tctgcacggt cgatagcatc cgggctggtg
     1381 ccgataaccg gtacgccagc agcttccagc gcgcgcgcca gtttcagcgg ggtctgaccg
     1441 ccgtactgaa cgataacgcc tttcggcttc tcaatacgca cgatttccag cacatcttcc
     1501 agagttaccg gctcgaagta gaggcgatcg gaagtgtcgt agtcggtgga gacggtttcc
     1561 gggttacagt taaccataat ggtttcgtaa ccgtcttcgc gcagcgccag cgaggcgtgt
     1621 acacagcagt agtcgaattc gataccctga ccgatacggt tcgggccgcc gccgagaacc
     1681 atgatttttt cacggtcggt agacggattc gcttcgcact cttcttcata agtggagtac
     1741 atgtaagcgg tgtcggtggc gaactctgcc gcacaggtat ccacgcgctt ataaaccggg
     1801 tgcaggtcat actggtcgcg cagcttgcgg atttccgctt cgcgcacgcc cgccagtttt
     1861 gccagacgcg catcggcaaa gcctttgcgt ttcagctggc gcaggaattc agcgttcagg
     1921 ccagtgatgc ccacttccgc tactttctct tccagacgca ccagttcttc aatctgtacc
     1981 aggaaccagc ggtcaatatt ggtcaggttg aacacgccgt ccacagacag gcccgcacgg
     2041 aaggcatcgg cgatgtacca gatacgctct gcgccagcat ctttcagttc gcgacggatt
     2101 ttggttaacg cttccgggtc atcgaggctc actttcgggt cgaatccagt cgcaccgact
     2161 tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg tgcgaccaat cgccatcact
     2221 tcgccaaccg atttcatctg agtggtcaga cggtcgttag caccagcgaa tttttcgaag
     2281 ttgaagcgag gaattttagt aaccacatag tcgatggacg gctcgaagga ggccggagta
     2341 cgtccgccag tgatgtcgtt catcagttcg tcgagggtgt aacccaccgc cagtttcgcc
     2401 gccactttag caatcgggaa accggtcgct ttcgacgcca gcgccgaaga acgggacacg
     2461 cgtgggttca tttcgataac aatcaggcga ccgtttttcg ggttcaccgc aaactggaca
     2521 ttggaaccac cggtttcaac gccgatttca cgcagcaccg ccatcgaggc gttacgcatg
     2581 atttgatatt ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga gtcaccggta
     2641 tggatgccca tcgcatcaaa gttttcgata gagcagacga tgatgcagtt gtcgttttta
     2701 tcacgcacca cttccatctc gtactctttc cagccgatca gcgactcatc aatcagcagc
     2761 tctttggttg gggagagatc cagaccgcgg gcgcaaattt cttcaaactc ttcgcggtta
     2821 taagcgatac cgccgccgct accgcccatg gtaaaggatg ggcgaataat gcacgggaag
     2881 cccacgtcag cggcaaccgc cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg
     2941 gtttccagac caattttctt catcgctacg tcgaaacgac ggcggtcttc tgctttatca
     3001 atcgcatcgg cggtcgcacc aatcatagtc acgccgaact cttccaacac gccctgacgc
     3061 tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca tcgttggcag caccgcgtcc
     3121 gggcgctctt tttcaataat cttgcgtact acttcccagt gaatcggctc gatgtaggtc
     3181 gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt tggagttcac cagaatgacg
     3241 cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc cagagtagtc aaactcacac
     3301 gcctgaccga taacaatcgg gcccgcaccc agaatcagga tactttttat atctgtacgt
     3361 tttggcatgg ctcttttact cctgattact tagcggtttt acggtactgc tcaattaact
     3421 cgataaagtg gtcgaacaac ggcgctgcgt cgtgtggccc agggctggct tccgggtgac
     3481 cctggaagct gaacgccggt ttatcagtgc gatgaatgcc ctgtaacgta ccgtcgaaca
     3541 gggatttatg cgtgacacgc aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt
     3601 ggttctgggc ggtgatcatc accacgtttt tctccacatc tttaaccgga tggttgccgc
     3661 cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc agcagctgat
     3721 gaccgaggca gatgccgaat accggaatat cggtttcgag gaatttctgg atggcggtaa
     3781 tggcgtaatc gcacggggcc gggtcgccag gaccgttgga gaggaagatg ccgtctggat
     3841 tcattttcag cacatcttcc gcagaagttt gcgccggaac gatggtcagg cgacagcctc
     3901 tatccaccag catccgcagg atgttgcgct tggcaccaaa atcataagcc acgacgtgga
     3961 acggcagctc gtcttctttt ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt
     4021 gtgtccagct ataggcttct gcggtggtca cttcttttgc cagatccatg ccattcagac
     4081 ctgggaacgc gcgggctttt tctaacgcca acgccgcatc cgggttatcg cctgcgataa
     4141 tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat
     4201 cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga gaggtcttcg gtattacgga
     4261 agttgctggc aatcagcggc agatcgcgaa tcaccagacc ttgtgcatgt acctgagaag
     4321 attcttcatc ggcgtcatta gtgccgacat tgccaatatg gggataagta agagtaacga
     4381 tttgacgaga ataggaagga tcagtgagga tttcttgata accggtcatt gaagtattga
     4441 aaacgacttc cccaaccgcc gaacctgttg cccctatggc ccgaccgtga aactgggttc
     4501 cgtcttccag aaccaatagc gctgacttaa tcaaaacacc ctccagagaa tattcactca
     4561 ctttatttgc atattaattc acaatgatga cgtaaatcaa tgcaaatctg cttaccgacg
     4621 aatttctggc aaacggcggc attctggaga tatgggcgct aaaagtcaac ttaatggcgg
     4681 tattttttat attattttgt ggctcatgca tgttttacag gcattaagcg acaaaaagat
     4741 catataacct gttctggaaa acgcttgcgc agcaagaaga ttgcatgaaa t
//
LOCUS       DAZPLM010000261         4765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPLM010000261 DAZPLM010000000
VERSION     DAZPLM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.263"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHVC7_003958"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHVC7_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR2117865.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHVC7_003959"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHVC7_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR2117866.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHVC7_003960"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHVC7_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR2117867.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHVC7_003961"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHVC7_003961"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR2117868.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHVC7_003962"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHVC7_003962"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR2117869.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4765
                     /locus_tag="ACHVC7_003963"
     CDS             4447..>4765
                     /locus_tag="ACHVC7_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR2117870.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEV"
BASE COUNT         1296 a         1223 c         1087 g         1159 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtat
//
LOCUS       DAZPLM010000262         4760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPLM010000262 DAZPLM010000000
VERSION     DAZPLM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.264"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            54..998
                     /gene="leuO"
                     /locus_tag="ACHVC7_003964"
     CDS             54..998
                     /gene="leuO"
                     /locus_tag="ACHVC7_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR2117871.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1153..1197)
                     /locus_tag="ACHVC7_003965"
     CDS             complement(1153..1197)
                     /locus_tag="ACHVC7_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117872.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1316..3040
                     /gene="ilvI"
                     /locus_tag="ACHVC7_003966"
     CDS             1316..3040
                     /gene="ilvI"
                     /locus_tag="ACHVC7_003966"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR2117873.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3043..3534
                     /gene="ilvN"
                     /locus_tag="ACHVC7_003967"
     CDS             3043..3534
                     /gene="ilvN"
                     /locus_tag="ACHVC7_003967"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR2117874.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3546..3632
                     /locus_tag="ACHVC7_003968"
     CDS             3546..3632
                     /locus_tag="ACHVC7_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR2117875.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3714..4718
                     /gene="cra"
                     /locus_tag="ACHVC7_003969"
     CDS             3714..4718
                     /gene="cra"
                     /locus_tag="ACHVC7_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR2117876.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1154 a         1092 c         1276 g         1238 t
ORIGIN      
        1 ttctctgcat tccaataagg gaaagggagt taagtgtgac agtggagtta agtatgccag
       61 aggtacaaac agatcattca gagacggcgg agttaagcaa gccacagcta cgcatggtcg
      121 atctcaactt attaaccgtt ttcgatgccg tgatgcagga gcaaaacatt acccgtgccg
      181 ctcatgttct gggtatgtcg caacctgcgg tcagtaacgc tgttgcacgc ctgaaggtga
      241 tgtttaatga cgagcttttt gttcgttatg gccgtggtat tcaaccgact gctcgcgcat
      301 ttcaactttt tggctcagtt cgtcaggcat tgcaactagt acaaaatgaa ttgcctggtt
      361 caggttttga acccgcgagc agtgaacgtg tatttcatct ttgtgtttgc agcccgttag
      421 acagcattct gacctcgcag atttataatc acattgagca gattgcgcca aatatacatg
      481 ttatgttcaa gtcttcatta aatcagaaca ctgaacatca gctgcgttat caggaaacgg
      541 agtttgtgat tagttatgaa gacttccatc gtcctgaatt taccagcgtg ccattattta
      601 aagatgaaat ggtgctggta gccagcaaaa atcatccaac aattaagggc ccgttactga
      661 aacatgatgt ttataacgaa caacatgcgg cggtttcgct cgatcgtttc gcgtcattta
      721 gtcaaccttg gtatgacacg gtagataagc aagccagtat cgcgtatcag ggcatggcaa
      781 tgatgagcgt acttagcgtg gtgtcgcaaa cgcatttggt cgctattgcg ccgcgttggc
      841 tggctgaaga gttcgctgaa tccttagaat tacaggtatt accgctgccg ttaaaacaaa
      901 atagcagaac ctgttatctc tcctggcatg aagctgccgg gcgcgataaa ggccatcagt
      961 ggatggaaga gcaattagtc tcaatttgca aacgctaact gaatgcagaa taggtcagac
     1021 atgaatgtct ggtttattct gtatttttta ttgaatgtag aattttattc tgaatgtgtg
     1081 ggctctctat tttaggatta attaaaaaaa tagagaaatt gctgtaagtt gtgggattca
     1141 gccgatttat tatcaattta atccttcgta atggaggatt ttatcgtttc ttttcacctt
     1201 tcctcctgtt tattcttatt accctgcgtt tatgtctctg gctgccaatt gcttaagcaa
     1261 gatcggacgg ttaatgtgtt ttacacattt tttccgtcaa acagtgaggc aggccatgga
     1321 gatgttgtct ggagccgaga tggtcgtccg atcgcttatc gatcagggcg ttaaacaagt
     1381 attcggttat cccggaggcg cagtccttga tatttacgat gcattgcata ccgtgggtgg
     1441 tatcgatcat gttttagttc gtcatgagca ggcggcagtg catatggccg atggtctggc
     1501 gcgcgcgacc ggggaagtcg gcgtcgtgct ggtaacgtcg ggtccagggg cgaccaatgc
     1561 gattactggc atcgccaccg cttatatgga ttccattcca ttagttgtcc tttccgggca
     1621 ggtagcgacc tcgttgatag gttacgatgc ctttcaggag tgcgacatgg tggggatttc
     1681 gcgaccagtg gttaaacaca gttttctggt taagcaaacg gaagacattc cgcaggtgct
     1741 gaaaaaggct ttctggctgg cggcaagtgg tcgtcctgga ccagtagtcg ttgatttacc
     1801 gaaagatatt cttaatccgg cgaacaaatt accctatgtc tggccggagt cggtcagtat
     1861 gcgttcttac aatcccacta ctaccggaca taaagggcaa attaagcgcg ctctgcaaac
     1921 gctggtagcg gcaaaaaaac cggttgtcta cgtaggcggt ggggcaatca cggcgggctg
     1981 ccatcagcag ttgaaagaaa cggtggaggc gttgaatctg cccgttgttt cctcattgat
     2041 ggggctggga gcgtttccgg caacgcatcg tcaggcactg ggcatgctgg gaatgcacgg
     2101 tacctacgaa gccaatatga cgatgcataa cgcggatgtg attttcgccg tcggggtacg
     2161 atttgatgac cgaacgacga acaatctggc aaagtactgc ccaaatgcca ctgttctgca
     2221 tatcgatatt gatcctactt ccatttctaa aaccgtgact gcggatatcc cgattgtggg
     2281 ggatgctcgc caggtcctcg aacaaatgct tgaactcttg tcgcaagaat ccgcccatca
     2341 accactggat gagatccgcg actggtggca gcaaattgaa cagtggcgcg ctcgtcagtg
     2401 cctgaaatat gacactcaca gtgaaaagat taaaccgcag gcggtgatcg agactctttg
     2461 gcggttgacg aagggagacg cttacgtgac gtccgatgtc gggcagcacc agatgtttgc
     2521 tgcactttat tatccattcg acaaaccgcg tcgctggatc aattccggtg gcctcggcac
     2581 gatgggtttt ggtttacctg cggcactggg cgtcaaaatg gcgttgccag aagaaaccgt
     2641 ggtttgcgtc actggcgacg gcagtattca gatgaacatc caggaactgt ctaccgcgtt
     2701 gcaatacgag ttgcccgtac tggtggtgaa tctcaataac cgctatctgg ggatggtgaa
     2761 gcagtggcag gacatgatct attccggccg tcattcacaa tcttatatgc aatcgctacc
     2821 cgatttcgtc cgtctggcgg aagcctatgg tcacgtcggg atccagattt ctcatccgca
     2881 tgagctggaa agcaaactta gcgaggcgct ggaacaggtg cacaataatc gcctggtgtt
     2941 tgttgatgtt accgtcgatg gcagcgagca cgtctacccg atgcagattc gcgggggcgg
     3001 aatggatgaa atgtggttaa gcaaaacgga gagaacctga ttatgcgccg gatattatca
     3061 gtcttactcg aaaatgaatc aggcgcgtta tcccgcgtga ttggcctttt ttcccagcgt
     3121 ggctacaaca ttgaaagcct gaccgttgcg tcaaccgatg atccgacatt atcgcgtatg
     3181 accatccaga ccgtgggcga tgaaaaagta cttgagcaga tcgaaaagca attacacaag
     3241 ctggtcgatg tcttgcgcgt gagtgagttg gggcagggcg cgcatgttga gcgggaaatc
     3301 atgctggtga aaattcaggc cagcggttac gggcgtgacg aagtgaaacg taatacggaa
     3361 atattccgtg ggcaaattat cgatgtcaca ccctcgcttt ataccgttca attagcaggc
     3421 accagcgata agcttgatgc atttttagca tcgattcgcg aggtggcgaa aattgtggaa
     3481 gttgctcgtt ctggtgtggt cggactttcg cgcggcgata aaataatgcg ttgagaatga
     3541 tctgaatgcg caatttgcag cccaacatgt cacgttgggc tttttttgcg aaatcagtgg
     3601 gaacctggaa taaaagcagt tgccgcagtt aattttatgc gcttagatgt taatgaattt
     3661 aacccatacc agtacaatgg ctatggtttt tacattttac gcaaggggca attgtgaaac
     3721 tggatgaaat cgctcggctg gcgggagtgt cgcggaccac tgcaagctat gttattaacg
     3781 gcaaagcgaa gcaataccgt gtgagcgaca aaaccgttga aaaagtcatg gctgtggtgc
     3841 gtgagcacaa ttaccacccg aacgccgtgg cagctgggct tcgtgctgga cgcacacgtt
     3901 ctattggtct tgtgatcccc gatctggaga acaccagcta tacccgcatc gctaactatc
     3961 ttgaacgcca ggcgcggcaa cggggttatc aactgctaat tgcctgctca gaagatcagc
     4021 cagacaacga aatgcgctgt attgagcacc ttttacagcg tcaggtcgat gccattattg
     4081 tttcgacgtc gttgcctcct gagcatcctt tttatcaacg ctgggctaac gacccgttcc
     4141 cgattgtcgc gctggaccgc gccctcgatc gtgaacactt caccagcgtg gttggtgccg
     4201 atcaggatga tgccgaaatg ctggcggaag agttacgtaa gtttcccgcc gagacggtgc
     4261 tttatcttgg cgcgctgccg gagctttctg tcagcttcct gcgtgaacaa ggtttccgta
     4321 ctgcctggaa agatgatccg cgcgaagtgc atttcctgta tgccaacagc tatgagcggg
     4381 aggtggctgc ccagttattc gaaaaatggc tggaaacgca tccgatgccg caggcgctgt
     4441 tcacaacgtc gtttgcgttg ttgcaagggg tgatggatgt cacgctacgt cgcgatggta
     4501 aactgccttc tgacctggca attgccacct ttggcgataa cgaactgctc gacttcttac
     4561 agtgcccggt gctggcagtg gctcaacgtc accgcgatgt cgcagagcgt gtgctggaga
     4621 ttgtcctggc aagcctggac gaaccgcgta agccaaaacc tggtttaacg cgcattaaac
     4681 gtaatctcta tcgccgcggt gtgctcagcc gtagctaagc cgcgaacaaa aatacgcgcc
     4741 aggtgaattt ccctctggcg
//
LOCUS       DAZPLM010000263         4744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPLM010000263 DAZPLM010000000
VERSION     DAZPLM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.265"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            20..1936
                     /gene="mngA"
                     /locus_tag="ACHVC7_003970"
     CDS             20..1936
                     /gene="mngA"
                     /locus_tag="ACHVC7_003970"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR2117877.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            1954..4587
                     /gene="mngB"
                     /locus_tag="ACHVC7_003971"
     CDS             1954..4587
                     /gene="mngB"
                     /locus_tag="ACHVC7_003971"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR2117878.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1147 a         1119 c         1319 g         1159 t
ORIGIN      
        1 cgatcaagtg aggatcatga tgaatctgac gactctgacc caccgcgatg cgttgtgtct
       61 gaatgcgcgc tttaccagcc gtgaagaggc catccacgcg ttgactcaac gtcttgctgc
      121 tctggggaaa atttccagta ctgagcaatt tctggaagaa gtgtatcgcc gtgaaagcct
      181 tggcccgacc gccttaggtg aagggttggc tgtgccgcat ggcaaaactg ctgcggtaaa
      241 agaagcggcg tttgcggtcg ccacactcag cgagccgctt cagtgggaag gcgttgatgg
      301 cccggaagca gttgatttag tggtgctgct ggcgattccc cccaatgaag cgggtactac
      361 gcatatgcaa ctgctgacag cgctgaccac gcgccttgcg gatgatgaga ttcgggcgcg
      421 tatacagtcg gcgacgacgc ctgatgagtt gctctcggcg ctggatgaca agggaggcac
      481 gcaaccttct gcctcttttt ccaacgcgcc aactatcgtc tgcgtaacgg cctgtccggc
      541 gggtattgct cacacctata tggctgcgga atatctggaa aaagccggac gcaaactcgg
      601 cgtaaatgtt tacgttgaaa aacaaggcgc taacggcatt gaagggcgtt taacggcgga
      661 tcaactcaat agtgcaaccg cctgtatttt tgcggctgaa gtcgccatca aggagagtga
      721 gcgttttaat ggcattcccg cgctttcagt gcctgttgcc gagccgattc gccatgcaga
      781 agcgttgatc caacaagcgc ttaccctcaa gcgtagcgat gagacgcgta ccgtacagca
      841 agatacgcaa ccggtgaaaa gtgtcaaaac ggagctgaaa caggcactgt tgagcggaat
      901 ctcttttgcc gtaccgttga ttgtcgcggg gggcacggtg ctggcggtcg cggtattact
      961 gtcgcaaatc ttcgggctac aagatctgtt taatgaagaa aactcctggc tgtggatgta
     1021 ccgcaagctg ggcggcgggc tgctcggaat tttgatggta ccggtgctcg cggcctatac
     1081 cgcctattct ctggcagata aaccggcgtt agcgccaggc tttgcggctg gacttgccgc
     1141 caacatgatc ggctccgggt ttctcggcgc ggtcgttggc ggattgatag ccggttactt
     1201 gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa ttcaatggat tcctgacttt
     1261 ttatctctac ccggtgctcg gtacgttggg agcgggcagt ctgatgctgt ttgtggtggg
     1321 agaacctgtc gcatggatca ataactcgct taccgcctgg ctgaacggtc tgtcaggaag
     1381 taacgcgctg ttgctgggtg ccattctcgg ttttatgtgt tcctttgacc ttggagggcc
     1441 agtgaacaaa gccgcttatg cattctgcct gggcgcaatg gcgaacggcg tttacggccc
     1501 gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt accgtaaccg cttccacgat
     1561 gctcgcaccg cgcctgttta aagagtttga aattgagacc gggaaatcca cctggctgtt
     1621 agggctggca ggtattaccg aaggggcgat cccgatggcg attgaagatc cgctgcgggt
     1681 tattggttcg tttgtgctgg gctctatggt aacgggcgct attgtcggtg cgatgaatat
     1741 cggcctttcg acacccggtg ccggcatttt ctcgctcttt ttacttcatg ataatggcgc
     1801 gggcggtgtt atggcggcaa ttggctggtt tggtgcggca ttggtggggg ctgcaatctc
     1861 gactgcaatt ctcctgatct ggcggcgtca cgcggttaag catggcaact atctgactga
     1921 tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag cggtatctcg cgttcacatc
     1981 accccgcata tgcattggga tcgggagtgg tatttcacca ccgaagagtc acgcattctg
     2041 ctggtcaata atatggaaga gatcctgtgc cgactggaac aggacaacga atacaaatat
     2101 tacgtactcg acgggcaaac ggcgatcctc gaagattatt tcgcggtgaa accggaaaac
     2161 aaagaccgcg tgaagaaaca ggtagaagcc ggtaagttga ttatcggccc ctggtatacc
     2221 cagaccgata ccacgattgt ttctgcggaa tccatcgtcc gtaatctgat gtacggaatg
     2281 cgtgactgcc tcgcgtttgg cgagccgatg aaaataggtt atttaccgga ttcctttggc
     2341 atgtccgggc aactgccgca tatttacaat ggatttggca ttacccgcac tatgttctgg
     2401 cgcggatgtt cggagcgcca cggtactgat aaaaccgagt ttttgtggca aagcagtgac
     2461 ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg ccatcggtaa gtacttacct
     2521 gccgacgaaa acggattacg taaacgcctc gacagttatt ttgacgtgct ggaaaaagcg
     2581 tctgtaacca aagagatttt gctgccgaat ggtcatgacc agatgccatt gcagcaaaat
     2641 atcttcgaag tgatggataa gctacgtgag atctaccctc aacgtaagtt tgtgatgagc
     2701 cgctttgaag aggtatttga gaagatcgaa gcgcagcgtg aaagtctggc caccctgaaa
     2761 ggtgaattta tcgatggcaa atatatgcgc gtacatcgca ccatcggttc cacgcgcatg
     2821 gatatcaaaa ttgcccacgc acgtattgaa aataagattg ttaatctgct ggaaccgctg
     2881 gcaacgctgg cctggacgtt gggttttgaa taccaccacg gcttgctgga gaaaatgtgg
     2941 aaagagatct taaaaaatca tgcccacgac agtatcggtt gttgttgcag tgacaaagtt
     3001 catcgcgaaa ttgtcgcccg cttcgaactg gctgaagaca tggcggataa tctgctgcgt
     3061 ttctacatgc gcaaaattgc cgataacatg ccacagagtg atgccgacaa actcgtgctg
     3121 tttaacctga tgccctggcc gcgggaagaa gttattaaca ccactgtgcg gctgcgcgcc
     3181 agccagttta atctacaaga cgatcgcggt cagcctgtac cgtattttat tcgccatgcc
     3241 cgtgagatcg atcctggcct aatcgatcgg caaattgttc attatggcaa ttatgatccc
     3301 tttatggagt ttgatattca gatcaaccag attgtccctt ctatgggcta tcgcacgctt
     3361 tatattgaag cgaatcagcc tggcaacgtg attgcggcaa aaagtgacgc tgaagggatt
     3421 ctggaaaatg ctttctggca gattgcgctc aatgaggatg gttctctgca actggtagat
     3481 aaagacagcg gtgtgcgcta tgaccgggta ttgcaaattg aagaaagctc tgatgatggt
     3541 gatgaatatg actattcacc cgcaaaagaa gagtgggtaa ttaccgcagc gaacgcgaaa
     3601 ccgcaatgcg atattattca tgaagcctgg cagagcaggg ctgttatccg ctatgacatg
     3661 gcagtgccgc tcaatttgtc agaacgcagc gcccggcaat ccactggcag agtagggggg
     3721 gtgttggttg tcactcttag tcataacagc aggcgtattg atgtggatat caaccttgat
     3781 aaccaggctg acgatcatcg ccttcgtgtc ctggtcccta caccttttaa cactgacagt
     3841 gttctggcag atacgcagtt tggttcgcta acgcgccccg tgaacgacag tgcaatgaac
     3901 aactggcagc aagaaggctg gaaagaagcg ccggttccgg tatggaatat gctcaactat
     3961 gttgccttac aggaagggcg taacggcatg gctgtcttta gcgaagggtt acgtgaattt
     4021 gaagtcatcg gtgaagagaa gaaaaccttt gccattacgt tgctgcgtgg cgtgggctta
     4081 ctgggcaaag aagatctgct tttaaggcct gggcggcctt cgggaattaa aatgccagtc
     4141 ccggactcac aactacgtgg tctgctttct tgtcgcctaa gtttattgag ttataccggt
     4201 acgccaaccg ccgctggtgt agctcagcag gcgcgagcat ggctgactcc agtacagtgt
     4261 tacaacaaaa tcccatggga tgcgatgaag ctcaacaaag ccggattcaa cgtgccggaa
     4321 agttatagtt tgttgaaaat gcccccagtg ggatgcctga taagcgcact taagaaagct
     4381 gaagaccgac aagaagtgat tttacggctg tttaatccgg ctgaatcagc aacctgtgat
     4441 gcgactgttg ctttcagtcg cgaggtgatt tcttgctcag aaacgatgat ggatgaacac
     4501 attaccaccg aggaaaatca aggttcaaat ctatcggggc cttttttacc cggccagtca
     4561 cggacgttca gttaccggct tgcctgaata gcaatcaaac cgaagccaca tatgcgcggc
     4621 cagattgttg acaaagggcg ctttgttcat gccggatacg gcatgaacgc tttattcggt
     4681 ctacaaaagc aggcaaattc aatatattgc agagatgatg tagacactgg caagcgtagc
     4741 gcat
//
LOCUS       DAZPLM010000264         4704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPLM010000264 DAZPLM010000000
VERSION     DAZPLM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.266"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHVC7_003972"
     CDS             complement(<1..737)
                     /locus_tag="ACHVC7_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR2117879.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHVC7_003973"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHVC7_003973"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR2117880.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHVC7_003974"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHVC7_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR2117881.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHVC7_003975"
     CDS             4397..4678
                     /locus_tag="ACHVC7_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR2117882.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1130 a         1171 c         1182 g         1221 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catt
//
LOCUS       DAZPLM010000265         4596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPLM010000265 DAZPLM010000000
VERSION     DAZPLM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.267"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHVC7_003976"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHVC7_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHVC7_003977"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHVC7_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR2117883.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHVC7_003978"
     CDS             complement(1327..3060)
                     /locus_tag="ACHVC7_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR2117884.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHVC7_003979"
     CDS             complement(3057..3551)
                     /locus_tag="ACHVC7_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR2117885.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHVC7_003980"
     CDS             complement(3673..4023)
                     /locus_tag="ACHVC7_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2117886.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHVC7_003981"
     CDS             complement(4066..4530)
                     /locus_tag="ACHVC7_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2117887.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..>4596)
                     /locus_tag="ACHVC7_003982"
     CDS             complement(4527..>4596)
                     /locus_tag="ACHVC7_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR2117888.1"
                     /translation="KQWKGLMTRREIEREVCLWGQQ"
BASE COUNT          993 a         1362 c         1152 g         1089 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgctta
//
LOCUS       DAZPLM010000266         4494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPLM010000266 DAZPLM010000000
VERSION     DAZPLM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.268"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHVC7_003983"
     CDS             <1..461
                     /locus_tag="ACHVC7_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR2117889.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHVC7_003984"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHVC7_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR2117890.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..4492)
                     /locus_tag="ACHVC7_003985"
     CDS             complement(2231..4492)
                     /locus_tag="ACHVC7_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR2117891.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1143 a         1050 c         1046 g         1255 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc ataa
//
LOCUS       DAZPLM010000267         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPLM010000267 DAZPLM010000000
VERSION     DAZPLM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.269"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..3057)
                     /gene="acrF"
                     /locus_tag="ACHVC7_003986"
                     /pseudo
     CDS             complement(1..3057)
                     /gene="acrF"
                     /locus_tag="ACHVC7_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3069..4226)
                     /gene="acrE"
                     /locus_tag="ACHVC7_003987"
     CDS             complement(3069..4226)
                     /gene="acrE"
                     /locus_tag="ACHVC7_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR2117892.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1120 a         1176 c         1093 g         1093 t
ORIGIN      
        1 gaagattgcc agcaacgttg cagagaccat tcctcccatt accccgatac ccactgcgtt
       61 ctgcgcgcca ctgccggcac cgttactgat agctagcggt aatacgccga gaataaaggc
      121 gagagaggtc atcaggatag gacgcagacg catacgtact gccatcagtg tcgcttcaac
      181 aacaccttta ccctttttct ccatgagatc tttagcgaac tcaacaatca aaatagcgtt
      241 tttggccgac aagccaattg tcgttagcaa gcccaccata aagtagacgt catttttttg
      301 attaaagagt gttgccgcca gtagcacgcc gacaatccct aacggcacga ccaacataac
      361 cgagacagga attgaccagc tttcatagag tgcagcaagg cacaggaaga caaccacaaa
      421 ggaaattgct accagagcgg gagcctggtt tcccgataag cgttcctgat acgacatacc
      481 tgtccagtca taaccaatgc ccgcaggtaa ttttgacgca aggttttcca tcaatgccat
      541 ggcatcgccg gaactggttc ctggcgcggc ttccccctga atctccattg acggcagacc
      601 gttgtagcgt tccagtcgcg gagagccata cacccaatgt gaagtggtaa aggcagagaa
      661 tggcaccatt tcgccgttgg cgctgcggac ataaagttta tcgacatctt ctggcagcat
      721 acggaatttg gcatccgcct gaacatacac ctttttcacg cgaccacggt cgatgaagtc
      781 gttaacgtaa gtcccaccca gcgccgttga aatggtctga ttgatgtcag aaagtgaaac
      841 acctaatgcc tgcgcctttt cctggtcaac ttccagttta aactgcgcgg tgtcttccag
      901 accattagga cgcacgctga ctaagctggc aggatgttgc gccgccatgc caagcaactg
      961 gttacgggcc tgggtcaggg catcgtgacc cagcccagcc tgatcaatta actcaaagtc
     1021 gaaacccgtt gccgtgccca gttcaacaat ggctggcata ttgaatggaa tgacaaaacc
     1081 gtcgcggatc ttgcccaatt ccattttggc acgatggatt accgcttccg cactgttttc
     1141 gtcaccacta cgctcttccc acggtttcag actgacgaaa gccataccgg cgttttgtgc
     1201 ctggccgctg aagctaaagc cgttaaccgt aaagacactt tctacgttcg ctttctcgtt
     1261 attcagatag taatccgtca cttgatccaa cactttttgc gtccgctctt gcgtcgcgcc
     1321 agcgggtaac tgaatcatgg tcagaaagac accctgatcc tcttcaggta agaaggaaga
     1381 cggaagacgt aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata
     1441 tcgtcctgtg gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc
     1501 gaaggtggta ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac
     1561 gggtttaagc agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag
     1621 cgccattgcc gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc
     1681 gaagaatgcc atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc
     1741 ctgaatttgc gacatcgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat
     1801 cacgcgctcg acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag
     1861 caccatcccg aacatcgtta gtgtgttgat ggagtaacca aaagcggcga ggatggcaaa
     1921 cgtccctaac aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg
     1981 caagaacaga tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac
     2041 ttcgtgaata gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc
     2101 ctgcgggaag aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag
     2161 agcgttcgcg ccggttgcta gcttaatccc caggcccgcc gccggttttc cgttgatacg
     2221 agcgataacg ttatagtttt caccgccaag ttcaactcgt gcgacatctt tcaggcgtac
     2281 caccgagccg tcactgttta cgcgcagggt tactttgccg aattcttccg gattttttaa
     2341 ccgcgtctga gcaataatcg aggcgttcaa ctgttgccct ggtaacgctg gcgttccgcc
     2401 caactgcccg gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg
     2461 tgtcagttta tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc
     2521 gccgaaaagc tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga
     2581 ggccacatag tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga caaagcccgc
     2641 caccatcaaa tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg
     2701 cggcagcaac ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg
     2761 atcggtcccg gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga
     2821 ggacatatac atcaggttat cgataccatt catattctgt tcgataacct gcgtcaccgt
     2881 atcctgcacg gtctgcgcat cagcacctgg atagtttgcc taaacagaaa ccgcaggcgg
     2941 tgcaatggtt ggatactgag cgacgggcaa ttgtaggatc gccagtgcgc ccgccatcat
     3001 cagaataatg gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt ttgccatgtc
     3061 agattacctt acttcgatgc agtatctgcg ggggtatcgg tagtggcttt aacctgctct
     3121 cccggacgcg ctttttgcag gccgctgaca atgacttgat cgccagattt cagaccttcg
     3181 ctaatcaacc atttatcgcc aatcgcctga ctggcaacga cagggcgcgc ttccacctga
     3241 cttttatcgt taacaatcag cacggttgca tcaccacgcg gtgtgcggct aacgccttgt
     3301 tgcgggataa gaatggcgtc aggttggacg ccttcatcaa tccgtgcacg cacaaacata
     3361 cccggcaaaa gcgtatgttg cgggttaggg aagacagcac gtagggttat ggagccggtg
     3421 cattcatcaa cggtcacatc agagaattgc agcgtacctt tcaggggata ggtttgaccg
     3481 ttttccatga ccaactctac gttgctggtg gcgttttcct tatgcaaatt tccttgctct
     3541 acggattgct tcagcctcat aaaatcgttg ctggattggg tcacatcaac gtagatagga
     3601 tcgagctgct ggacagtcgc cagttcagtc gtttgcccat tagtgacaag agcgccttcg
     3661 gtcacggtcg atttgccgat acgtccgcta attggcgcag tcactttggt ataagcgaga
     3721 ttgatgcgag cgctttcgac tgtggctttt gcggcaatca cggcggcatc ggcctggcga
     3781 gcatcagcaa tggcctggtc gtactcctgc tggctgatgt atttcgtacc cacgagcgga
     3841 acgtaacgtt ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt cgccagttcg
     3901 cctttcgcgc tgtcataatt tgcctgatag gtcgcgggat cgatctggta cagggactgg
     3961 cctgcttgta catcgctgcc ttcagtgaaa ttacgattca gtacgatccc gctaacctgt
     4021 gggcgaactt cggctatacg gtaagcattg gtgcggcctg gtaattcagt cttaacttct
     4081 aacggggccg ttttcacaat atgaacggta acctgcggtt caccgacgtg agctttctct
     4141 tctcccttat cgttacaacc gacgattaaa gccgcggaga tcagaataaa ggagggcagg
     4201 aggaaaaacc tggcatgttt cgtcattact attcctcaaa aaaacaaaag cgcgttattt
     4261 acccagaaag gcaacgcgtt aactcgcaga aagaaaaata cagtttgcta tcctacaaat
     4321 tatcattcgt cgatgtaagg aatagttatg aatactggcg tctcaaggca cataaacaca
     4381 aaaaaagatt aatattctac tgttttattt tgacgcgggt tgaaagaggc agaattaaaa
     4441 cctcgtaaat tgaaatatat attcatgtgg cgaatgtatt tt
//
LOCUS       DAZPLM010000268         4481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPLM010000268 DAZPLM010000000
VERSION     DAZPLM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.270"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHVC7_003988"
     CDS             complement(8..1069)
                     /locus_tag="ACHVC7_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR2117893.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHVC7_003989"
     CDS             complement(1066..2463)
                     /locus_tag="ACHVC7_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR2117894.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHVC7_003990"
     CDS             2618..3616
                     /locus_tag="ACHVC7_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2117895.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHVC7_003991"
     CDS             complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHVC7_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR2117896.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc g
//
LOCUS       DAZPLM010000269         4457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPLM010000269 DAZPLM010000000
VERSION     DAZPLM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.271"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHVC7_003992"
     CDS             complement(<1..264)
                     /locus_tag="ACHVC7_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR2117897.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="ACHVC7_003993"
     CDS             493..1101
                     /locus_tag="ACHVC7_003993"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR2117898.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="ACHVC7_003994"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="ACHVC7_003994"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR2117899.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="ACHVC7_003995"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="ACHVC7_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR2117900.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          995 a         1136 c         1322 g         1004 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaat
//
LOCUS       DAZPLM010000270         4437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPLM010000270 DAZPLM010000000
VERSION     DAZPLM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.272"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            123..1205
                     /locus_tag="ACHVC7_003996"
     CDS             123..1205
                     /locus_tag="ACHVC7_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR2117901.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1394..1777)
                     /gene="quuD"
                     /locus_tag="ACHVC7_003997"
     CDS             complement(1394..1777)
                     /gene="quuD"
                     /locus_tag="ACHVC7_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR2117902.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1863..2003)
                     /locus_tag="ACHVC7_003998"
     CDS             complement(1863..2003)
                     /locus_tag="ACHVC7_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR2117903.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2000..2362)
                     /gene="rusA"
                     /locus_tag="ACHVC7_003999"
     CDS             complement(2000..2362)
                     /gene="rusA"
                     /locus_tag="ACHVC7_003999"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR2117904.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2359..2649)
                     /locus_tag="ACHVC7_004000"
     CDS             complement(2359..2649)
                     /locus_tag="ACHVC7_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR2117905.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2642..2812)
                     /locus_tag="ACHVC7_004001"
     CDS             complement(2642..2812)
                     /locus_tag="ACHVC7_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR2117906.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2812..3267)
                     /locus_tag="ACHVC7_004002"
     CDS             complement(2812..3267)
                     /locus_tag="ACHVC7_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR2117907.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3532..3909
                     /locus_tag="ACHVC7_004003"
     CDS             3532..3909
                     /locus_tag="ACHVC7_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117908.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            3906..4334
                     /locus_tag="ACHVC7_004004"
     CDS             3906..4334
                     /locus_tag="ACHVC7_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117909.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1241 a         1003 c          944 g         1249 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat cacattgatt
       61 agatgaatat ttatcgcgca gtgacatcat tttttaataa tagttcaaaa aaagggctca
      121 cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt
      181 ctgctcaggc agctgaaatt tataataaag acagtaacaa gctggatctg tacgggaaag
      241 ttaatgctaa gcactacttc tcctctaatg atgcagatga tggtgatact acttatgccc
      301 gtcttggctt caaaggtgaa acccaaatca acgatcaact gactggtttc ggtcagtggg
      361 aatatgaatt caaaggcaac cgcgctgaat ctcaaggttc ctccaaagat aaaacccgtc
      421 ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattacggc cgtaactacg
      481 gtgtagcata cgacatcggt gcgtggactg acgtcctgcc agaattcggt ggtgacactt
      541 ggactcaaac agatgtgttc atgactggtc gcaccactgg tgttgcaact tatcgtaaca
      601 acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag ggtaaaaatg
      661 accgcactga cgtaactgaa gctaatggtg atggtttcgg tttctccact acttatgagt
      721 atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat aatcaggtta
      781 tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc
      841 tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc cagaacatga
      901 ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta gttgcacaat
      961 atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact
     1021 tgggtgcgtg gggtgatcag gacctggttg aatatattga tgtaggtgca acctattact
     1081 tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat aagagcgatt
     1141 tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggtatg gtttaccagt
     1201 tctaatttga ttactaaaag atatgttgcg ggaggctttg cctccccaac atataagtgg
     1261 ctccctcaag ccacttcctt taggagcaca accttgcttc taactatata aaccttctgt
     1321 tatatattac cctttatttt tgggggcgtt gcaacgcccc atttttaata atttttagta
     1381 aacaattggc atattaatta gagttattaa caacgatatc catctctaac cggatatcta
     1441 atgccattaa catcccttca attatgccct cagccttctg taaccttttc ccgatataac
     1501 catcagagca gcaatgctta cctgccagtg acatgaatgt cataccgact acataataat
     1561 ctactaataa atcgtgcaaa tcgctgttgt tctttttcag acgggccatg cacccgcaaa
     1621 taatcatcgc gtcatcgtca caacattgcg ggcgagattt tacttttgaa gtaattaatc
     1681 ccttaaaacc ggcggcaatg gacgaccagg tcacatcttc atgattatta gccgcccacg
     1741 ctccccaacg ctcaagaacc atctgaatat cacgcatcaa cttactccac aaaaatcaga
     1801 ccagaacgcc aattacaagc aaaaatcaac aaaacagtat tagttgattg ttatctctga
     1861 cttcatactc ctgctcctgt cagggttttg gcgtaattct tcagtattcg gtaatcggtc
     1921 aaaacagaac cggggaaacg atataagcgc agatgccccc agcggtggcg aagaagttct
     1981 gccatataaa actcaaacat cattcattcc ccatttcggt gatggtcagt tccagcctcc
     2041 cacctttggt aacaggcatc ttcacaacgc ggtaatcaac gacctgagca tcatccagcc
     2101 agaaacctgc tttggtgagt gcgtcaaaag cggctttttg cagattatcc aggtcacggc
     2161 gacggcgatc cggcatgtgg cactcaatac ggattttcac tggcatagcc aggccgatat
     2221 ccagcattgc gtttttaatg attcgggtga cgttatcgcg gtatgcctgc ccttctgcgc
     2281 tgatgtgtgt gcgcccgcga ttatggcggt aatagcggtt attgctcgga ggccagggta
     2341 atgtgatgct gtaggtattc acgccttaat aaccccctct ttcagccaga taacctgcgt
     2401 tctcgccata ccttccagcg cgcattcttt tgcatactca gcatcgacaa aatatgtgcg
     2461 gcggtcgatt tcgtcgtgac aggcagaaca tgcaatggtg gcaatcaggt ctggcggttt
     2521 gataccggta ccgcacaatc cagccagccg gatatgtgcc agtactgacg tttcaggatt
     2581 gccattacat acgccaggga ttcttacctg gcattcccga ccacgcgctg cttttctcaa
     2641 atcagccatg attcctcctt gctgccagtc gcaaccattt tttatcaacc aggctagcgg
     2701 tatatccgag cagtgttggt atttcggatg gcttcagctc aggcttacgc ttacgacgat
     2761 ttgatactct gtagatgtgc ccgttcatga cacgaataag cggtgtagcc attacgcctc
     2821 ctgcttatcg cgcagctgct ggtattcaca gctctgcgga attgtcaggt ggcagccgat
     2881 attcatcgcc caggcttcga ctttgcacag gaagatgtac atctcgccgg tttccagctc
     2941 tgacgtatgg cggagggatt ggacggtggt gacctcgccg gacacgacat ctacacggtc
     3001 tttgctttcg tagccgagat aggtgtgctt catcgcgtct ttgacccact caggcgtagc
     3061 gaaggtcttg ccgcgggcga tgagatattc gctgatctcc gtgtaccaca tgtggctgag
     3121 cgcgttctgc gacaggctgc gcttctcgcg ccacggctta acctgcaggc gtaagcattg
     3181 cccggcatcc agcaatggct gaatctgctg gccgatggcc gcgaagttac cgcgatggag
     3241 tttgatgccg tctactggca gagtcatacg gcctccctaa cggaaaccgc agaatgcaga
     3301 aaatcgcagg tgcatttctg catctgtgac aaggtgatga gttcagattg tggtcgcatt
     3361 taagtcccct taaatgcgca gaagtcaccg gagttgttca ggctccgatg agaggattat
     3421 ggcggattga ttacgataaa tcaaaactat cttgaagcca aaacagtgat cttcgcttta
     3481 aaaaccttgt atactttcac ctaatcacag ccagaactat ggatctaacc aatgaccaac
     3541 aaagaaaaca aagataaata tattaacaag ttcaagaaag agctttcaaa atatatacaa
     3601 ccaactgctg gagtggacat tcaattattt aactctaagg atggtggtgg tgtaattaaa
     3661 gccactttga atagaagtgg aaaaagaaaa tcatctattg ctggtaattt tactaaatta
     3721 ggtgaggcaa ttacctcgag tgggcagagg gtttttggtg gtgatctatc aaatgtgaat
     3781 ttttacggaa caaatacgat ttttgatgga gatacaatat ttttaattaa atcacctgat
     3841 tcagaggagt ggtcatcaca aaaagcttcc aaagacgtag agggcatcgt atttggaggg
     3901 aaaaaatgat taacattact actaaggatg gcactcaagt cagtggtgaa gtaagagatc
     3961 tagtaattct tcttgaaact gatggaaaaa cggtgtctga ttatttgcag ccaatccctc
     4021 ccaaaaaaga tacacactgg agcatgattt tcattccggt agcaatttat atactactta
     4081 atcttgtata cattattgct cacccattat ttcctgaaaa atatctaaaa acatcaacat
     4141 ctatatttct attcatttcc ttcatattaa gtgcaatagt agcatatttt gttttccata
     4201 agcataaatc tgccactcta tccatatgca gcttagtgtt cttagtgtta attgtaggtg
     4261 ctaacgtagg gtttatcagt tacagcgacc taacacaaaa agcgacgcaa aaattagata
     4321 gtttgtctaa ataattatgt tgccatgcga agtatgcatg gctgcatgtc tgccttccat
     4381 ttaaaatggg ctaagaccta taaccctaaa tattattctt tattatcttc tttacca
//
LOCUS       DAZPLM010000271         4410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPLM010000271 DAZPLM010000000
VERSION     DAZPLM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.273"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHVC7_004005"
     CDS             complement(<1..105)
                     /locus_tag="ACHVC7_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117910.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHVC7_004006"
     CDS             complement(173..2122)
                     /locus_tag="ACHVC7_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2117911.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHVC7_004007"
     CDS             complement(2207..2542)
                     /locus_tag="ACHVC7_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR2117912.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHVC7_004008"
     CDS             complement(2542..2898)
                     /locus_tag="ACHVC7_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR2117913.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHVC7_004009"
     CDS             complement(2898..4394)
                     /locus_tag="ACHVC7_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR2117914.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat
//
LOCUS       DAZPLM010000272         4378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPLM010000272 DAZPLM010000000
VERSION     DAZPLM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.274"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHVC7_004010"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHVC7_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR2117915.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /locus_tag="ACHVC7_004011"
     CDS             1447..3156
                     /locus_tag="ACHVC7_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR2117916.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(3208..>4378)
                     /gene="yicI"
                     /locus_tag="ACHVC7_004012"
     CDS             complement(3208..>4378)
                     /gene="yicI"
                     /locus_tag="ACHVC7_004012"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR2117917.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT          967 a         1199 c         1174 g         1038 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccgcgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg tggggctgac ctccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggttt tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg taattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttaaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggcg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggttcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaatgtcacg tttggtcgtg atggccagcc cgcgaccctg
     1681 gtggccaaaa gtgtcgacat tgcgctaagt agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcgcg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaacct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaagaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttct
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgcggc aattgccgga
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgacg
     2761 tttaatcgtg ttgatgtccg tcgtccgtcg ctggcgctaa ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacacatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac agtggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggccaatgtc ccgttgaaac ctacggttag cgggcaattg
     3061 catgccgtga acgccgcaaa gcaccaagtg actcaaacca tgaatgcggg cgtcgtttcc
     3121 agcggtgaag ttacatcggc agagctggtg cagtaaacat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattctt caatagatta caacgtaatt gtcagcgcat tcccttgagg
     3241 cgtaaccacc agcccctgct cactttcagc ctgcgaaccg ccttgcagac catttacttt
     3301 cacaatattg cgcaagcaca gtgtccagtt cctcgcctcg cccgcaccag tcacagtaat
     3361 cgtgttacca gtacgtgttg cttttagagt aaagatcacc gaaccgtcag cagcgggcac
     3421 ttcacataca gcttcatgcc cgtcttgcag attaaagaga tggaatgccg tgccttcgtg
     3481 ccacgcgtaa tcgggacgtt gatcgttgtt gcccagcgcc aatagggtgt tatcacgcac
     3541 ataaacgggc agactcagga agccgtgctg ctgtttatgc cagcgactac cgtcgagctc
     3601 attgttgtgc cacaggtgtg tccagcgacc ttccggcaag tagaactgca cctcgcccgc
     3661 ctcagtgaac accggagcaa ccatcacgtt gtcgcctagc atgtattgac ggtcaaggta
     3721 atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg gcgtaccccg
     3781 cgctttcgca cgcgcagctt cacgatacag atacggcatc atgcggcatt tcagttgcgt
     3841 gaagaagcgc accacatcac aggactcatc atcgtaggcc cacggcacac gataagattt
     3901 gctgccatgt aaacgactat ggctggagag caaaccaaac gcgcaccagc gtttgtaaac
     3961 gtgtgccggc gcggtatttt caaagccgcc gatatcgtgg ctccagaagc caaaacctga
     4021 aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt tagcgtaaca
     4081 atcgccaccc cagtgtaccg ggaatttctg cgcaccgacg gaggccgagc gggcaaacaa
     4141 gacagcttct tcctcaccaa cggtgtcctt gagcacgttc cacaccagtt cgttgtagat
     4201 gtacgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact gaacatcagt
     4261 tgggatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga ccagaccttt
     4321 cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa tcgccaga
//
LOCUS       DAZPLM010000273         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPLM010000273 DAZPLM010000000
VERSION     DAZPLM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.275"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHVC7_004013"
     CDS             complement(<1..215)
                     /locus_tag="ACHVC7_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR2117918.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHVC7_004014"
     tRNA            complement(330..405)
                     /locus_tag="ACHVC7_004014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHVC7_004015"
     tRNA            complement(412..486)
                     /locus_tag="ACHVC7_004015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHVC7_004016"
     tRNA            complement(603..687)
                     /locus_tag="ACHVC7_004016"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHVC7_004017"
     tRNA            complement(696..771)
                     /locus_tag="ACHVC7_004017"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHVC7_004018"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHVC7_004018"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR2117919.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHVC7_004019"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHVC7_004019"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR2117920.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHVC7_004020"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHVC7_004020"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR2117921.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHVC7_004021"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHVC7_004021"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZPLM010000274         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPLM010000274 DAZPLM010000000
VERSION     DAZPLM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.276"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHVC7_004022"
     CDS             complement(<1..583)
                     /locus_tag="ACHVC7_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2117922.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHVC7_004023"
     CDS             complement(646..1095)
                     /locus_tag="ACHVC7_004023"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR2117923.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHVC7_004024"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHVC7_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZPLM010000275         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPLM010000275 DAZPLM010000000
VERSION     DAZPLM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.277"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHVC7_004025"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHVC7_004025"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR2117924.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHVC7_004026"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHVC7_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR2117925.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHVC7_004027"
     CDS             2269..2976
                     /locus_tag="ACHVC7_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR2117926.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHVC7_004028"
     tRNA            3082..3157
                     /locus_tag="ACHVC7_004028"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHVC7_004029"
     CDS             3355..>4309
                     /locus_tag="ACHVC7_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2117927.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPLM010000276         4289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPLM010000276 DAZPLM010000000
VERSION     DAZPLM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.278"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..572)
                     /locus_tag="ACHVC7_004030"
     CDS             complement(<1..572)
                     /locus_tag="ACHVC7_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR2117928.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
     gene            complement(783..2180)
                     /gene="ccp"
                     /locus_tag="ACHVC7_004031"
     CDS             complement(783..2180)
                     /gene="ccp"
                     /locus_tag="ACHVC7_004031"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR2117929.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2585..4234
                     /locus_tag="ACHVC7_004032"
     CDS             2585..4234
                     /locus_tag="ACHVC7_004032"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR2117930.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1101 a         1097 c         1012 g         1079 t
ORIGIN      
        1 gggtccataa acaactgacc ggggcgcata gggatctcac gcagctccac atcccagtag
       61 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ccaggtttgg tttatccgtt
      121 ggtttgcctg cagcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
      181 atacaggcct cggaagaacc aatggtgttg gtgccaacgg cctgaccatt tttcggcgca
      241 ggcgcatgcc acagatcggc aaccatattt acgcaacgca ggtcgatggc tgcggattgc
      301 ggatattctt ctttgtcgat ccagttttta ttgatcgaca aatccatcaa tttatggacg
      361 ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct gacgagcgtt gccatcaaga
      421 tataattcat cattgataat ctggaatgcg acatcatcgc gcatttcgtg cagcggaaat
      481 cgttttgact ccgcgatagt agaaatggcc tttgcgccaa aacgtgaatc gagtagttct
      541 gagcggaaat ccgttaacag cttctggtcc atttcgaact ccttaaattt atttgaaggc
      601 aataaaaaag taggatttat ccgcaatgga agcaaggcat tacaggctta atttaaataa
      661 caaaatccta agcagaaaaa cgacttttta taatataaac atttaacatg ataatattaa
      721 aaacagactt tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt
      781 aattattgtt tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct
      841 acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt
      901 ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca
      961 acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc
     1021 atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagccccaac
     1081 ggttcaaagg aacgtccacc gagaataata ccaccatgac aagttgcaca tttattatct
     1141 ttaaataatt gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac
     1201 catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca
     1261 gtaatatttt cgccactgaa accttgcgga tagacgccga ggaactgcgc tttaagctgc
     1321 ggatcttttt ccagcttagc aataatttcg tcccaagatt tcgacgccat ttcaatcggg
     1381 ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc
     1441 tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca
     1501 acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg
     1561 catgaaatgg tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc
     1621 actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc
     1681 tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt
     1741 atttccgccc gctcttcatc actcacctta cccgcccagt gaagcgcggt ataacgcgtt
     1801 ggcggcatag tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg
     1861 ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc
     1921 ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct
     1981 gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga gaaaacctaa aaccttatta
     2041 ttttcactga cagcggatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac
     2101 cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta
     2161 atacgtgaga ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa
     2221 attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagtgagat
     2281 acgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa gatagcctgt
     2341 gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt
     2401 ctgaaaatga aaaacaacac gaaatccgta tttttataaa ttccatggat attagtgcgt
     2461 tatatttttt gacaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg
     2521 agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat
     2581 ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaagtcga
     2641 tctctgctat gagctggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc
     2701 ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta
     2761 tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc ccggacagta aaacctttcc
     2821 cgactgcgca cctaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg
     2881 tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta
     2941 ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg
     3001 gccggtgcta acccgcgaac cacaggatca cattccgtgg tcttctctac tggcgctgcc
     3061 gcagtcatat attgtcccag gcggccgttt tagcgaaacc tactattggg actcctattt
     3121 caccatgctg gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa
     3181 cttcgcctgg atgatcgaaa actatggtca catccccaac ggcaaccgca cctattattt
     3241 gagccgatcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt
     3301 acgcggtgcg cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg
     3361 tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc
     3421 gctgctcaac cgttactggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga
     3481 cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc
     3541 gggggcggcc tccggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc
     3601 gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga
     3661 gagcgccatc gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg
     3721 ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg
     3781 catctaccgc gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat
     3841 tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt
     3901 acgcagccgg ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca
     3961 gtgggataaa cccaacggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat
     4021 gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca
     4081 gttctatctg gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg
     4141 cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt
     4201 acgccgttta attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc
     4261 ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZPLM010000277         4262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPLM010000277 DAZPLM010000000
VERSION     DAZPLM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.279"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..3512)
                     /locus_tag="ACHVC7_004033"
     CDS             complement(<1..3512)
                     /locus_tag="ACHVC7_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR2117931.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(3964..>4262)
                     /locus_tag="ACHVC7_004034"
     CDS             complement(3964..>4262)
                     /locus_tag="ACHVC7_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2117932.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1099 a         1095 c          878 g         1190 t
ORIGIN      
        1 acctgacgcc tctcaagact attataaata gcctcaatct cacccttcag gttatcaccg
       61 caataaacca ttgatgtatc gtatcgttca tatcgcgctt cccgtgtcgc actaaaccag
      121 ttgaacgatc ccacacacag caaaccatca tcaccaataa caattttgct atgaacacgg
      181 tttaccagct ttgtagcaat acccagcgca ttcagtttct ccagcgccgc tttaaagttc
      241 tgctgcttct cttttcgctt ctcaaaatca ttatgttcag tgttgtagct tctgtcagtg
      301 actatcgtga cgttaattcc acgtgaacac gccgcaatca tggaatcaag aaaaccggtt
      361 tgctccagcc tttgccaggt cagccatgga gaaattatcg tgatgtgttt actggtattt
      421 tcaaatgtct gattcaggaa attatcatgt tgctccacac catgaagtgt gtagattttg
      481 gtcccggcgg tttttaaatc cttacgctct ttataatcaa aagagagcgc attcttctct
      541 gactcaaaga ggtattttgc cagtaatccc cgtggcgatg aggctggctg gacctcaaac
      601 aggtccatat cgccgaagac cagaaaactg tccttcgcac gggagactgc aacattcagc
      661 atgctgttat cgctatcaat aaacccgccg tcttcatgtt ttgaatagac tggcgagaat
      721 atcacaatcg ctctttccgc tccctgaaga gagtgcacgg tgcccactgt gagcgacttt
      781 tcattcgtgc ctgtactgat accttgtttg cccagcacct gtttgatagt ggatacctga
      841 gcgctaaaag gcgtcacaat accgacaact tcatgaagcg atttaccgta atgcgcttca
      901 atattttgct ggttctctgc caaccagacc gctatcgttt cagcctcaag caaattatat
      961 cgacttccac tacttgccag ctctccttta ccatcaatat ggagataccc cattgcgggc
     1021 attaaattgc tctcttcacg ccctctttta ggcaacaact taccgtgata gcagagcgta
     1081 ttacagtatc caataatatt gtcgtagcac cggcggtgtt catataggta cataccacga
     1141 gccagttcgg gatcatattg atagcgcgaa gcaaactgcg ctattttcat aacgctgcca
     1201 gatgcggcac ttttaccaag gtctgcgatt gccgtatatt tctcggtaat ctcttcttgc
     1261 gtactgccag acagaatttt ttccgccagc atgttaccga catcaatcgc aggagcaata
     1321 ctccatattg gcgggatctg ctccgtatcg ccaatcacta atgccttctt agctaatgca
     1381 aacgaggcag cagccacttc aggaagcacc tgcccggctt catcgacaat gagtaaatcg
     1441 gcaaaatcat acaaataact tttctcgaat ttacgttgtc ctttgtgctc acttatctgc
     1501 atattaccgg gcagcatata gcatgtcatt accacacatg gcgtgagttt cattcgacgt
     1561 tgccaacggg cggttacccc tttagcacct tttttcttct tctcgtcctg cagatcatca
     1621 atgctggcca tatccatcag ccaacgacct tcccagtagt gagtcgtcag taaaaatgca
     1681 gggaagcgaa tctgcgtatc agccagttca tcggcctgtg agaagctcag ttcctcgtcg
     1741 ccctcatacc ctaaatcaaa tgccagcctc tgccactcct gaaccgcctg ctgttcttta
     1801 agaacgattt catgggcgga gtcaatctgc tgccggtatg ttgtttgctc gcgctcagcg
     1861 gaattgagca gcccatcaat attacgttcg atagtttcag gatcagacca ctgatttcct
     1921 gcaatcagcg cgcctaattt atcttcgaga aacagctgta tttggtactg tcgcttattg
     1981 cgaaccgccg ggagccagga aaataatgaa tagatcagtg attcaccggc gcgatatttt
     2041 ttccattccg ttttagcact cttcagtaga gtgacttttt gttcttgtcc ggaaagtaat
     2101 ttatttaaat tatcgagata ttgctcaata tcattagcaa taagctcacg cgcagcccga
     2161 atctggctta acgtttgcca tgttgcgttc agtcttatca gttgctcaga ttttgctgcc
     2221 aactgaccat gcaggagttc aatgaccttt tcaggggatg aacactcttt tccaggaaag
     2281 gctgccttag ccttttccag ataaaacagc agtgcatcct ctacatactc ttttgactca
     2341 acctggttga agaaatcttc agtttgatat tttttggctg cctcagcttt acgactgctt
     2401 gagggaaaat aagcaccgaa gcttttcagc tctggcaacc atcgcccggc catcgcacct
     2461 gaaccttgcg aaaagtcttt cccgaatgcc tcaataatgt tcgttacagc ctggttattc
     2521 gttgaagtcg cgataataac cggaggctca gatttttcga gagccgctct ggcccactgc
     2581 gtggcgatga tagaaagcac cagcgtggtt tttccggttc ccggagggcc attaacagca
     2641 aggatatcgc catgtcttgc atccagaaaa tggcttaagg catcgcgctg agcctttgcc
     2701 agcggaaact tatctccgga gtgtccaagc ctgtcgctga attttgctcc tggggccagc
     2761 aaagactctg cagcatgaac ctctcgcgag gcgaagcgat tgaagagcgg cacatcctta
     2821 ttgcaaacaa tcaggtgatc ataaagagaa aggatatggg aactggcacc gccagattga
     2881 gccgttttaa caatataacc gtgctcagcg agttcatatt gctcaggttt ttcaatccag
     2941 tcgccggcaa cgctcttcag tagcctctct gagtcataca gatattgacg ccactcctgc
     3001 tgcaaggcgg catatcgtgc ttcccgttct tcatccgttt cggcagtctt atcaacgctg
     3061 tcatcaaagt tgatagagaa cgtggtatgg gtcgtcttgt atttgtcata ctgcccaatc
     3121 tcaccaatcg aaaatgctcc ttttggcaaa ggttcaagca ggtctctggg aatggaggtc
     3181 gctggcgtcg gatataaaaa accctcacgg cttagcaagg ctggcgtcac tatcggggta
     3241 acaatatcag gcgcacctgc agaacggtcc ttaccatgct gcagtaaccg gaaataaact
     3301 tttgggcgca agatgacatc gaccgtttcg acatcgtctt tttctccctt aaaaaattta
     3361 ctgacaatcg cttcgtcaag acgtcccgcc gctatcccat gccagtgagt gaaatttttg
     3421 gcgtctttcc gttcaaaact gccctttcct gactcagcat ccgcaagcga gttgcgccag
     3481 tatgaggtaa accctaaagc attttcatcc attaccagtc ctgtgactca atgaaatcca
     3541 ttgatttatc gaatataaaa ccaccatatg gacaactatt gtccatattg aaaaagttgg
     3601 atgcaatgtt ttgtttataa ttcggtatca aatgtactgg ttgtacaagg ttcagaacac
     3661 aagcacattc aacagtacgt caggactatt gatttcataa cagtgaatac attttttacc
     3721 gtttataatg actgcctgtg ttggacaagg ataccagaac agccgaaaac accgggggtt
     3781 attacctgct ctcatggaaa ccagatattc ctggtgctaa tcacagtcat aaatagcctg
     3841 aagaacatga cgaagggact ttaatggaag ccggagtaac ttgccagaac atcaatcttc
     3901 gtcatcattt acaaagaaaa ataatcagtt aaagcagtcc gggcaatcag cccggactgc
     3961 ttactatttt aatggattgt taatctttgc atattcaaac gggctgatac ccttatctct
     4021 gttcgcatcc agaaaatctg cccaccactg tagcatcagt cgacgttctt ccagatgttc
     4081 tgctttatgg atatacgcag cacgtacacc attacgctcc tgatggctca tctgacgttc
     4141 cacagcatcg cgggaccaca aacctgattc aatcaatgca ctgcacgcca tcgcccggaa
     4201 tccatggcca cagacatcct gggttgtgtc ataccccatc accctcaggg ctttatttac
     4261 ag
//
LOCUS       DAZPLM010000278         4196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPLM010000278 DAZPLM010000000
VERSION     DAZPLM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.280"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHVC7_004035"
     CDS             complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHVC7_004035"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR2117933.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHVC7_004036"
     CDS             complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHVC7_004036"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR2117934.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHVC7_004037"
     CDS             complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHVC7_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR2117935.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHVC7_004038"
     CDS             complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHVC7_004038"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR2117936.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3216..3731)
                     /locus_tag="ACHVC7_004039"
     CDS             complement(3216..3731)
                     /locus_tag="ACHVC7_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2117937.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1070 a         1072 c         1046 g         1008 t
ORIGIN      
        1 tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct
       61 gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aacccccccg gcgaatgtac
      121 gcagctacat taattccgcc gttcgaatgc cagtgctttg cgctcgatca gacgcatcat
      181 cagcgtcagc aggccgttga cgaccaggta aataatccct gccgcaccga acaccattac
      241 atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca gcgtaatggt
      301 gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag aagagagcga
      361 gcgtttaaag gcatacggca gcaggagcgc cagcgtatct tttttgctca ttcccagggc
      421 gctacaggac tgccactgac cttctgggat cgcacgaatt gcaccgtaaa acagctgcgt
      481 ggtatacgcc gcactgttca gcgataacgc aatcagcgca cataaccacg gttctgacaa
      541 caaatgccac agtgccggat actcctgcaa cgtcggaaac tggcccggcc cgtaataaat
      601 caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac cccgcaccag
      661 ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag
      721 tgccacaatc agcgaggcaa cggttagcgt caggctggtg tgtagccctt tcatcagttc
      781 gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg
      841 cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg
      901 taccaggtaa atggttcctg ggtacgagta gcgatgcttt ttgtttgcag cattaaatca
      961 ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg
     1021 aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat
     1081 ttcgacagcc ccagcgcctg accagactcc cactgaccca ccggcaccgc tttcaacgcg
     1141 ccccgaagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa
     1201 aggaacgggc taacgtcgaa gttctcaatg tccatctgca ctgggatctg cacgaaccca
     1261 agattgatag tgaagccatc cgaaagcgtc agcagcagct gcgaggagcc aaaatagata
     1321 aacagcacca ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc
     1381 cacgcgacag gacgccattt tgccgactcc cataccgcaa agaacatcgc cagcgccagc
     1441 ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga
     1501 aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg
     1561 ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagctc agtgttgccc
     1621 tgacgtaccg cgatgccgag gccagtgccg aagtaatctt tatcggtcac tttgtcgccc
     1681 accgccgcca gtttcgggtt atctttcagc cactcagtga ccactgcggt gtcaccgaag
     1741 acgccgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct gtcatacgga
     1801 acggtagtga tttccgggtg cttatccata atgaatttct ggtgtgtcgt cccgttctgt
     1861 acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca
     1921 aacagggcag agttgtcata gtacggggtg gtaaacagca cctgcttttc acgctccgga
     1981 gtgatatcca tgcccgccat cacggcttct acgcgacgga atttcaggct tgggatcagg
     2041 ctgtcaaacg cctggttaga gaaagtgcag gttgcatcaa tctctttaca cagcgcttgt
     2101 gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga
     2161 taggatgctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag actaaaacct
     2221 gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga
     2281 gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta
     2341 cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt
     2401 cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga
     2461 tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg
     2521 gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca
     2581 gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt tctgcacggg
     2641 ccagcgcctg atctttactc aaccccagta cacggcaggg cgcttcaatc aggttttgct
     2701 gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca
     2761 aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt
     2821 tgagcgtacc ggagcgtggc atctcaagca gattgagtac acgcagcagc gagcttttac
     2881 ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc agcgtgatat
     2941 cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg
     3001 acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct ttgacagaat
     3061 aattatgcaa tatttctgct ttaaaagtta aaagcaaagc gcattattca ataaacatag
     3121 cacaaaataa cgggggcggt ggtcggcgag cataaatgtc ggcattcctc acgaaatgcc
     3181 ggacaattta cggggtttat tggttgatca aggcgttagc gattctcgat ggactgacgg
     3241 agcgtacccg ccgtggcatg aacgctgccg cctaagtagc gcacatcgtc gatgacccaa
     3301 cactggcctt cctgaatcat taacacttca tcctgccaac cctggtcacc ctgtttgaga
     3361 tctacgcgca atggaatgtt acgggcatca cgattaggga tagtcgatgc actggcaacg
     3421 tgggcgctat ctggcaaggt ggttcgactg gagaacggat cgctgcttaa caattcacga
     3481 tgactattat cgcggctggc atcgctaaga agggttgcca gtttgtcact caaatagggg
     3541 cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcatagaa ttgttgagcc
     3601 acgctgtcag gccccccctg gacacaggaa ccggtgcggg gaccgttatc tttataagct
     3661 ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag agatagtgaa
     3721 cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac gaccaaacgc
     3781 tatcagatta tttaatacat tgtttttaat acaattctga aacaatctga acacctgatt
     3841 gggtttgagt ccattgctgg ctatccactc aaggttgctc gtagctgact tacttaaatt
     3901 acccaaacaa tagacagtgt aaaccatttg tgaggttgag cgtgttatat ggctgaaatg
     3961 atcatttaag gtaaatatga tagttctgaa atggtggtat aaatcctcct gtagaggtta
     4021 agagcatctg caagaagcca gtttcacatt tataacgcgt gataaatgaa cgcggttatt
     4081 gtctaatcat tacaaaattt ttcacatgcg ataagagatc ctgcgattaa gatgattgcc
     4141 aaaattacgt tgtatttttg aaaatgagcg aaatttgata gtcccagctt tgactc
//
LOCUS       DAZPLM010000279         4184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPLM010000279 DAZPLM010000000
VERSION     DAZPLM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.281"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..3853
                     /gene="sigA"
                     /locus_tag="ACHVC7_004040"
     CDS             <1..3853
                     /gene="sigA"
                     /locus_tag="ACHVC7_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR2117938.1"
                     /translation="KIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSIYG
                     SYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVPDF
                     SAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRLNK
                     FVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLFGQ
                     NYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHGVT
                     STVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVSGG
                     GELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGDNL
                     HKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSESGN
                     GEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGNVS
                     GNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGNNN
                     CPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNLEN
                     ATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSAGE
                     SSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIELA
                     GTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILFGV
                     DQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQSLK
                     TRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGSNN
                     SLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEHTT
                     WTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARIMS
                     GAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSVGA
                     GLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHVTE
                     DSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLEFE
                     KSAFGKYNVDNAINANFRYSF"
     gene            3912..>4184
                     /locus_tag="ACHVC7_004041"
     CDS             3912..>4184
                     /locus_tag="ACHVC7_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117939.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
BASE COUNT         1364 a          833 c          911 g         1076 t
ORIGIN      
        1 taaaatttat tcactgaaat atagtcatat tacaggtgga ttagttgctg tttctgaact
       61 gacccggaaa gttagtgtcg gtacatcaag aaagaaagtt atcctcggta ttattttatc
      121 ctcaatatat ggaagttatg gcgaaacagc atttgcagca atgctggata taaataatat
      181 atggacccgc gattatcttg accttgctca aaacagagga gagttcagac cgggtgcaac
      241 aaatgttcaa ttaatgatga aagatggaaa gatatttcat tttccagaac tacctgtacc
      301 tgatttttct gctgtttcca acaaaggtgc aacaacatca attggaggtg cgtacagtgt
      361 tactgcgact cataacggta cacagcatca tgcaataaca acacagtcat gggatcagac
      421 agcatataaa gcaagtaaca gagtatcatc tggcgacttt tcggttcatc gtctgaataa
      481 attcgtcgtg gaaacaacag gggttacgga gagtgccgac ttctcacttt ctcccgaaga
      541 tgcgatgaaa agatatggcg taaactacaa cggtaaggaa caaataattg gcttcagagc
      601 aggtgccgga acaacctcaa cgatattaaa cggcaaacaa tatctgtttg gacaaaacta
      661 taatcccgac ttgttaagcg caagtctttt taatctggac tggaaaaaca agagttacat
      721 ttataccaac agaacccctt ttaaaaactc accaattttt ggcgatagtg gttctggttc
      781 ttatctatat gataaagaac aacaaaaatg ggttttccat ggtgttacca gtacagttgg
      841 ttttctcagt agtaccaata tagcctggac aaactactcg ttatttaata atattctggt
      901 aaacaattta aaaaagaatt tcacaaacac tatgcagctg gatggtaaaa aacaagagtt
      961 atcatcgatt ataaaagata aggacctgtc tgtctcagga ggaggggaat taacgctcaa
     1021 gcaggatacc gatcttggca ttggcgggct tatattcgat aagaaccaga catataaagt
     1081 gtacggaaaa gataagtctt ataaaggtgc cgggatagat attgataata ataccaccgt
     1141 tgaatggaat gttaagggcg ttgccggaga taatctgcat aaaataggta gtggtactct
     1201 ggatgtaaaa atagcacagg gaaataacct taaaataggt aatgggactg tcatccttag
     1261 tgctgaaaaa gccttcaata aaatttacat ggccggaggt aaaggtacgg taaaaataaa
     1321 tgccaaagac gctttaagcg aaagcggtaa tggcgaaatc tattttacca gaaatggcgg
     1381 aacactggat ctaaacggct atgaccagtc atttcagaaa atcgcagcaa cagatgcggg
     1441 aacaaccgta acgaactcaa acgtgaagca atcaacatta tcacttacta atactgatgc
     1501 atatatgtac catgggaatg tatcaggtaa tataagcata aatcatatta tcaatactac
     1561 ccagcaacat aacaataatg ccaatctgat ctttgatggc tcagtcgata tcaaaaacga
     1621 tatctctgtc cggaatgcac agttaacatt acaaggacat gcgacagaac atgccatatt
     1681 taaagaaggc aataacaact gtccaattcc ttttttatgt caaaaagact attctgctgc
     1741 cataaaggac caggaaagca ctgtaaataa acgttacaat acggaatata agtccaacaa
     1801 tcagatagcc tctttttccc agcccgactg ggaaagtcgt aaatttaatt tccggaaatt
     1861 aaatttagaa aacgcaaccc tgagtatagg ccgggatgct aatgtaaaag gacacataga
     1921 ggctaaaaac tctcaaattg ttctgggaaa taaaactgca tacattgaca tgttctcagg
     1981 aagaaacatt actggcgaag gttttggatt cagacaacag cttcgctccg gggattcagc
     2041 aggcgaaagt agtttcaacg gcagtctgag tgctcaaaac agcaaaataa ctgttggtga
     2101 taaatcaact gttactatga ctggtgcatt atccttaatt aatacagacc tgattatcaa
     2161 caaaggagct actgttaccg cccagggaaa aatgtatgta gataaagcta ttgaactggc
     2221 cggaaccctg acattaacag gcacccctac agaaaataat aaatacagcc cggcaatcta
     2281 tatgtcagat ggatataata tgacagaaga tggtgccacg ttaaaggctc aaaattatgc
     2341 ctgggtcaat ggtaatataa aatcagacaa aaaagcatct attctgtttg gtgttgacca
     2401 gtataaagaa gataacctgg acaaaaccac acacacaccg ctggctacag gtttgctggg
     2461 tggctttgat acttcttata ccggaggtat tgatgctcct gctgcctcag ccagcatgta
     2521 taacacctta tggagagtaa acggacagtc agccctgcaa tcattaaaaa cccgcgacag
     2581 tcttttgttg tttagtaaca tagagaattc gggtttccat actgtgacag taaacacact
     2641 ggatgccact aatactgctg tgattatgcg ggctgatctg agccagtctg taaatcaatc
     2701 ggataaactc attgttaaaa atcagttaac cggaagcaat aacagtctgt cggtcgatat
     2761 acagaaagtg ggaaataata actcaggatt aaacgttgac ctgataacag ccccaaaagg
     2821 aagcaataaa gagatattta aagccagtac tcaggccata ggtttcagca acatatctcc
     2881 tgtgatcagc acgaaagagg atcaggaaca taccacgtgg accctgaccg gatataaggt
     2941 ggctgaaaat acagcatctt ccggtgcagc aaaatcgtat atgtccggta attacaaagc
     3001 cttcctgaca gaagtcaaca acctgaataa acgaatgggg gatctgcgtg acaccaatgg
     3061 cgaggccggt gcatgggccc gcatcatgag cggagcaggt tcagcttcta gtggatacag
     3121 tgacaactac acccatgtgc agattggtgt ggataaaaaa catgagctgg atggacttga
     3181 ccttttcact ggtctgacta tgacgtatac cgacagtcat gccagcagta atgcattcag
     3241 tggcaagacg aagtccgtcg gggcaggtct gtatgcttcc gctatatttg actctggtgc
     3301 ctatatcgac ctgattagta agtatgttca ccatgataat gagtactcgg cgacctttgc
     3361 tggactcgga acaaaagact acagttctca ttccttgtat gtgggtgctg aagcaggcta
     3421 ccgctatcat gtaacagaag actcctggat tgagccgcag gcagaactgg tttatggggc
     3481 cgtatcaggt aaacggttcg actggcagga tcgcggaatg agcgtgacca tgaaggataa
     3541 ggactttaat ccgctgattg ggcgtaccgg tgttgatgtg ggtaaatcct tctccggtaa
     3601 ggactggaaa gtcacagccc gcgccggcct tggctaccag tttgacctgt ttgccaacgg
     3661 tgaaaccgta ctgcgtgatg cgtccggtga gaaacgtatc aaaggtgaaa aagacggtcg
     3721 tattctcatg aatgttggtc tcaacgccga aattcgcgat aatcttcgct tcggtcttga
     3781 gtttgagaaa tcggcatttg gtaaatacaa cgtggataac gcgatcaacg ccaacttccg
     3841 ttactctttc tgataacagc ccgggccgcg tttgcggccc ttcttctacc gtagagaata
     3901 tgtattaccc tgtgacagac tatatcgctc ttgctctcat tattagcttt ctttttctga
     3961 cattatttat ctgcctgtta tgccttaaac atgagcgaat aaaaaaagaa actatcaggc
     4021 aaaaaaatgc acatattctg gagcatggct ggaatgcaac tgagttctca tggttccgat
     4081 acggacagta taacgaaacg ggtatttaca tctcgatcga gaagaccatt atcattacaa
     4141 tcaccgtttc tggtgagtgc tttaaaaaag aatacagcat tgtc
//
LOCUS       DAZPLM010000280         4180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPLM010000280 DAZPLM010000000
VERSION     DAZPLM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.282"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHVC7_004042"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHVC7_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR2117940.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHVC7_004043"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHVC7_004043"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR2117941.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHVC7_004044"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHVC7_004044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR2117942.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4087..>4180
                     /locus_tag="ACHVC7_004045"
     CDS             4087..>4180
                     /locus_tag="ACHVC7_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117943.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLS"
BASE COUNT         1030 a         1062 c          966 g         1122 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcaggt
     3841 catttcgtaa ttacattaat taaaaaactt aaaaccatta aataaataaa aaaccactaa
     3901 ctctatgtga aataaatcaa aatttcacgc tgcaatactc cttagaatgt atagcgaaag
     3961 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcaa
     4021 cttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4081 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4141 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
//
LOCUS       DAZPLM010000281         4111 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPLM010000281 DAZPLM010000000
VERSION     DAZPLM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.283"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHVC7_004046"
     CDS             <1..122
                     /locus_tag="ACHVC7_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2117944.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHVC7_004047"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHVC7_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR2117945.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="ACHVC7_004048"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="ACHVC7_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR2117946.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHVC7_004049"
     CDS             3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHVC7_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR2117947.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT         1165 a          891 c          932 g         1123 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa actttattgt
     3241 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
     3301 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
     3361 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga tttactgaaa
     3421 ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg cctgttcctg
     3481 cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc
     3541 ttaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
     3601 ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
     3661 ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
     3721 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
     3781 cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
     3841 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
     3901 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
     3961 gtcaaagtca catgggtatg aatacgggca acacgatgag tgctttagct atttgattat
     4021 ttgctaacga gtagtcaacc acgcacgctg cgtaagaatt aaatggggca gccattccct
     4081 gccccgcgtt gtttttaggc gatatattta t
//
LOCUS       DAZPLM010000282         4098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPLM010000282 DAZPLM010000000
VERSION     DAZPLM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.284"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(261..908)
                     /gene="ychE"
                     /locus_tag="ACHVC7_004050"
     CDS             complement(261..908)
                     /gene="ychE"
                     /locus_tag="ACHVC7_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR2117948.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1386..4061
                     /gene="adhE"
                     /locus_tag="ACHVC7_004051"
                     /gene_synonym="adhC"
     CDS             1386..4061
                     /gene="adhE"
                     /locus_tag="ACHVC7_004051"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR2117949.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1137 a         1007 c          947 g         1007 t
ORIGIN      
        1 ttattattta gcactttcct ctaccaaagg cagtggaatt actaatatca tttcaattat
       61 tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag gagaagcagt
      121 taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta ttccttttgc
      181 ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga gcatggcagc
      241 tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt aataccagta
      301 acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt aatcacgttg
      361 atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa tccccagcaa
      421 cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat gctgtgataa
      481 cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa cggcaacgcc
      541 aatggcacca caccaatgct ttcacgtacc gcggtttctg atttttcttg cttgttctgt
      601 ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac caggatgccc
      661 cctgcgatac ggaacgaatc aattgatata ccaaaaagtt gcagaatcgt gtcgccgaga
      721 aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt agttttgttt
      781 cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat ccctaccggg
      841 ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc caaaaaggtc
      901 tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat taaggttgaa
      961 aagacgcgct gacaatacgc cttttgacag catttttcac ctcctaacta cttaaaattg
     1021 ctatcattcg ttattgttat ctagttgtgc aaaacatgct aatgtagcca ccaaatcata
     1081 ctacaattta ttaactgtta gctataatgg cgaaaagcga tgctgaaagg tgtcagctta
     1141 gcaaaaattt gatttggatc acgtaatcag tacccagaag tgagtaatct tgcttacgcc
     1201 acctagaagt gacgcattag agataataac tctaatgttt aaactctttt agtaaatcac
     1261 agtgagtgtg agcgcgagta agcttttgat tttcataggt taagcaaatc atcaccgcac
     1321 tgactatact ctcgtattcg agcagatgat ttactaaaaa agtttaacat tatcaggaga
     1381 gcattatggc tgttactaat gtcgctgaac ttaacgcact cgtagagcgt gtaaaaaaag
     1441 cccagcgtga atatgccagt ttcactcaag agcaagtaga caaaatcttc cgcgccgccg
     1501 ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat ggccgttgcc gaatccggca
     1561 tgggtatcgt cgaagataaa gtgatcaaaa accactttgc ttctgaatat atctacaacg
     1621 cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga cgacactttt ggtaccatca
     1681 ctatcgctga accaatcggt attatttgcg gtatcgttcc gaccactaac ccgacttcaa
     1741 ctgctatctt caaatcgctg atcagtctga agacccgtaa cgccattatc ttctccccgc
     1801 acccgcgtgc aaaagatgcc accaacaaag cggctgatat cgttctgcag gctgctatcg
     1861 ctgccggtgc tccgaaagat ctgatcggct ggatcgatca accttctgtt gaactgtcta
     1921 acgcactgat gcaccaccca gacatcaacc tgatcctcgc gactggtggt ccgggcatgg
     1981 ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt aggcgcgggc aacactccag
     2041 ttgttatcga tgaaactgct gatatcaaac gtgcagttgc atctgtactg atgtccaaaa
     2101 ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt tgttgttgtt gactctgttt
     2161 atgacgctgt acgtgaacgt tttgcaaccc acggcggcta tctgttgcag ggtaaagagc
     2221 tgaaagctgt tcaggatgtt atcctgaaaa acggtgcgct gaacgcggct atcgttggtc
     2281 agccagccta taaaattgct gaactggcag gcttctctgt accagaaaac accaagattc
     2341 tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt cgcacatgaa aaactgtccc
     2401 cgactctggc aatgtaccgc gctaaagatt tcgaagacgc ggtagaaaaa gcagagaaac
     2461 tggttgctat gggcggtatc ggtcatacct cttgcctgta cactgaccag gataaccaac
     2521 cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacggc tcgtatcctg attaacaccc
     2581 cagcgtctca gggtggtatc ggtgacctgt ataacttcaa actcgcacct tccctgactc
     2641 tgggttgtgg ttcctggggg ggtaactcca tctctgaaaa cgttggtccg aaacacctga
     2701 tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt gtggcacaaa cttccgaaat
     2761 ctatctactt ccgccgtggc tccctgccaa tcgcgctgga tgaagtgatt actgatggcc
     2821 acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa caatggttat gctgatcaga
     2881 tcacttccgt actgaaagca gcaggcgttg aaactgaagt cttcttcgaa gtagaagcgg
     2941 acccgaccct gagcatcgtt cgtaaaggtg cagaactggc aaactccttc aaaccagacg
     3001 tgattatcgc gctgggtggt ggttccccga tggacgccgc gaagattatg tgggttatgt
     3061 acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg ctttatggat atccgtaaac
     3121 gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat gatcgctgtc accaccactt
     3181 ctggtacagg ttctgaagtc actccgtttg cggttgtaac tgacgacgct actggtcaga
     3241 aatatccgct ggcagactat gcgctgactc cggatatggc gattgtcgac gccaacctgg
     3301 ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct ggacgcagta actcacgcca
     3361 tggaagctta tgtttctgta ctggcatctg agttctctga tggtcaggct ctgcaggcac
     3421 tgaaactgct gaaagaatat ctgccagcgt cctaccacga agggtctaaa aatccggtag
     3481 cgcgtgaacg tgttcacagt gcagcaacta tcgcgggtat cgcatttgcg aacgccttcc
     3541 tgggtgtatg tcactcaatg gcgcacaaac tgggttccca gttccatatt ccgcacggtc
     3601 tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa tgcgaacgac aacccgacca
     3661 agcagactgc attcagccag tatgaccgtc cgcaggctcg ccgtcgttat gctgaaattg
     3721 ccgaccactt gggtctgagc gcaccgggcg accgtactgc tgctaagatc gagaaactgc
     3781 tggcatggct ggaaacgctg aaagctgaac tgggtattcc gaaatctatc cgtgaagctg
     3841 gcgttcagga agcagacttc ctggcgaacg tggataaact gtctgaagat gcattcgatg
     3901 accagtgcac cggcgctaac ccgcgttacc cgctgatctc cgagctgaaa cagatcctgc
     3961 tggataccta ctacggtcgt gattatgtag aaggtgaaac tgcagcgaaa aaagaagccg
     4021 ctccggctaa agctgagaaa aaagcgaaaa aatccgctta atcagtagcg ctgtctggca
     4081 atataaacgg ccccttct
//
LOCUS       DAZPLM010000283         4097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPLM010000283 DAZPLM010000000
VERSION     DAZPLM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.285"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..2272
                     /locus_tag="ACHVC7_004052"
     CDS             <1..2272
                     /locus_tag="ACHVC7_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2117950.1"
                     /translation="HLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCY
                     PIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASH
                     YAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPI
                     PAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVT
                     VSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRK
                     GQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGV
                     GFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKN
                     FVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYS
                     LSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDN
                     KISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRD
                     SYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIG
                     VIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIR
                     LADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWDDKSCFIQPPNS
                     SNLTTGTVILPCISQN"
     gene            2288..3037
                     /locus_tag="ACHVC7_004053"
     CDS             2288..3037
                     /locus_tag="ACHVC7_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2117951.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3039..>4097
                     /locus_tag="ACHVC7_004054"
     CDS             3039..>4097
                     /locus_tag="ACHVC7_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117952.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1263 a          838 c          843 g         1153 t
ORIGIN      
        1 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
       61 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
      121 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
      181 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
      241 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
      301 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
      361 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
      421 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
      481 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
      541 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
      601 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
      661 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
      721 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt
      781 cacgcaatgg caagttgcat ctaatagtat tccttatctg acgcgtaaag ggcagattcg
      841 ctacaccact gctatgggta aaccgaccag cgttggtggt gattccttac aacaaccctt
      901 cttctggact ggtgaattct catggggttg gctgaacaat gtatccctgt atggtggttc
      961 agttttaaca aaccgtgatt atcaatctct ggctgccggc gttggcttta accttaactc
     1021 attgggttca ttatcttttg atgtcacacg atctgatgct cagttgcata atcaggataa
     1081 agaaacgggt tatagctacc gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca
     1141 gctcactttc gctggttacc gtttctctga taaaaacttt gtgacaatga atgaatatat
     1201 caatgacact aaccattaca tgaattatca gaatgaaaaa gagagttata ttgtcacgtt
     1261 taaccagtat cttgaatcat taaggttaaa tacatacgta agtttggctc gtaatactta
     1321 ctgggacgcc agcagtaatg tgaattattc attatcactt agccgcgatt ttgatatcgg
     1381 gccattaaaa aacgtctccg cttctctaac atttagccga ataaactggg aagaagacaa
     1441 ccaggatcaa ctgtacctaa atatttcgat tccctgggga accagtagaa cattgagcta
     1501 tggtatgcaa cgaaatcagg ataacaagat ttcgcatact gcttcgtggt atgactcttc
     1561 cgatcgaaat aattcctgga gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat
     1621 gaaagcgtca ctacgcgcca gttatcagca taataccgag aacggtcgtc tctatctctc
     1681 cgggacatca cagcgagaca gttattattc tctgaatgcc agttggaatg gttcattcac
     1741 ggcgactcgt cacggagccg ctttccatga ctatagtggt agtgctgact cgcgttttat
     1801 gatcgacgca gatggtgcag aagatattcc gttgaacaat aaacgcgcgg taactaatcg
     1861 gtatggcatc ggagttattc catcagtcag cagttacata acaacatcat taagcgttga
     1921 tacccgaaat cttccagaaa atgtggatat cgaaaactcg gttatcacca ccaccttaac
     1981 cgaaggtgct attggctacg ccaaacttga tacccgcaag ggctatcaaa tcatgggggt
     2041 tattcgcctg gcagatggta gtcacccacc actggggatt agcgtaaaag ataaaaccag
     2101 ccataaagaa ttaggactgg ttgctgatgg cggctttgta tacctcaacg gcattcagga
     2161 tgacagcaaa cttactttac gctgggatga taaatcctgt tttattcaac cacccaatag
     2221 cagcaactta accaccggaa cggttatttt accgtgtatt agccaaaatt aattcaggat
     2281 gaaactaatg cgctacttaa acacaaagaa tctcattgca gcaggagtat tactcgcctg
     2341 catgagcagc attgcctggg gtgccatcat cccggaccgt acccgtatca taatgaatga
     2401 gtcagataaa ggggaagcat taaaacttac taatcaaagc aaaaatctac cctatctggc
     2461 gcaaacatgg atagaagata cgaaaggcaa taaatcacgt gactttattg tcacggttcc
     2521 ccctatggta cgtttaaatc ccagtgagca aattcaaata agaatgataa gtcaggaaaa
     2581 aattgctcaa ctgcctaatg acagagaaac gctcttctat tttaacgtgc gagaaatacc
     2641 gccaaaaaca gataaaaaaa atgtgatgca agtcactatg cagcacgcat tgaaactatt
     2701 ctggcgacca aaagctattg aattagaaga tgatggggta atgtcttacg aaaaagttga
     2761 aatcatccgt agaaatgatg gttcgatccg ctttaacaat aagatgcctt atcacgttac
     2821 gctgggttat atcggaacaa acggaatcac aatgttgcca caaacgcaaa gtttaatggt
     2881 aaccccttat agccatgcag atacgcagtt taaaaatgtt ccgtcagctt ttcaggttgg
     2941 ttatatcaat gattttggtg gattaagttt ttatgaaata aactgcccga cggttaataa
     3001 ctcttgtaat gtttccgtag ccaaacgaga taaataatat gcgctactta ttaattgtaa
     3061 ttatatttat tataggtttt agtgcattac ctgtatgggc aatggactgc tatgctgaac
     3121 atgaaggggg aaataccgta gtcattggtt acgtaccaag aatttctatc cccagtaatg
     3181 gtaaaaaagg tgataagatc tggcaaagca gtgagtattt tatgaatgtc ttctgtaata
     3241 atgcattacc tgccccatca cctggagaag aatacccatc agcctggact aatataatga
     3301 tgtttttacc aggcggtcag gacttttata atcagaactc ctatattttt ggcgtaacat
     3361 acaatggagt tgattatgat tcgactgccc ctaatgctat cgcagcacca gaatgcattg
     3421 atataaaagg agcgggtacc tttaataatc actataaaaa ccccgctgtc tgtagtggag
     3481 gtcctgagcc tcaattatca gtaacctttc cagctcgcgt acaactctat attaaattag
     3541 caaaaaatgc caatagagtt aataaaaacc tggtgttacc tgatgaatat atcgctctag
     3601 agtttaaagg tatgagtggc gcaggagcta tagaagtaga caaaaatttg accttcagga
     3661 ttcgtggatt aaataatatt catgtccttg actgctttgt taatgtcgca ctggaaccag
     3721 ctgatggcgt tgttgacttt ggtaaaataa attcccgaac aattaaaaat accagcgtta
     3781 gtgagacgtt tagtgtagtc atgacaaaag atcctggtgc ggcctgtact gagcaattca
     3841 atattttagg gagctttttc actacggata ttttgagtga ttatagccat ctggatatgg
     3901 gtaatggact gctattgaag atatttcata aagatggaac agcaacggaa tttaaccgct
     3961 tctcacaatt tgcttctttt tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg
     4021 cagaacttag tgcgaacccg gcagagactg ttgttgaggg accgtttagt aaagacgtaa
     4081 tcctcaaaat cacctat
//
LOCUS       DAZPLM010000284         4072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPLM010000284 DAZPLM010000000
VERSION     DAZPLM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.286"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            75..776
                     /locus_tag="ACHVC7_004055"
     CDS             75..776
                     /locus_tag="ACHVC7_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2117953.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHVC7_004056"
     CDS             complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHVC7_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR2117954.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3360..3614
                     /gene="mobC"
                     /locus_tag="ACHVC7_004057"
     CDS             3360..3614
                     /gene="mobC"
                     /locus_tag="ACHVC7_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR2117955.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          962 a         1030 c         1066 g         1014 t
ORIGIN      
        1 aaaaaaaaaa cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatagc
       61 aacgagcaag cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa
      121 gctacaagct cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctgaaat
      181 ccggagaaga ttccccttcg ccggatgaac aaaaaaccat gaccatcacg gctgcagagt
      241 ttcatgatgc ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga
      301 tagatcgcct ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc
      361 gctgggtgca gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct
      421 ccgatgccat catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga
      481 agaacgtctc cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt
      541 tccgcagcac cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg
      601 aagataaata tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg
      661 aactgaacga aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg
      721 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      781 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      841 actattgaag tgaaaaattg cgtcaaaaac gctctgatgc agcgaaaaaa gaccccccaa
      901 tttttggggg cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca
      961 tccctgtcga tgaacatcga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     1021 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     1081 gtgcatcttc ctcgcgctgc cattcctccc aggtacgatt aaaacccagc gagcggaaca
     1141 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     1201 tcagatgaaa ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta
     1261 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat
     1321 gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa
     1381 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     1441 ggtcgacaga actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca
     1501 ccttccttgc cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     1561 ccggccatgt cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct
     1621 gcgtcagcag ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc
     1681 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa
     1741 agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt
     1801 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc
     1861 ccgtaacgac tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct
     1921 gctccgcgcg actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt
     1981 taagcaactg ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt
     2041 caaatgccat ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta
     2101 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     2161 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     2221 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     2281 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt
     2341 tactgtcctt cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag
     2401 gaagcccgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc
     2461 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     2521 cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     2581 taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     2641 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg
     2701 catgtgtccg gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc
     2761 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     2821 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     2881 cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     2941 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     3001 caggggcggt ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt
     3061 cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag
     3121 ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca
     3181 tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa
     3241 acggagtgga gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag
     3301 tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca
     3361 tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg
     3421 cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgctgca ttgctggcaa
     3481 aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag
     3541 aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc
     3601 tgccagaaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag
     3661 cgagtgggcg aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt
     3721 tcggcaggtg atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg
     3781 tgtattcgaa acttgcaatt ctgctttatt tcaacgcatt gtttttattg gttttttgtt
     3841 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
     3901 ctttttttta aaaccttttt aggtcttgta acaccatgat tttaaagatg ttctggcaag
     3961 ataagtggag cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg
     4021 ggggtgaaag gtggagcttt tgggggtgta atccacagaa aaagggggtt gc
//
LOCUS       DAZPLM010000285         4072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPLM010000285 DAZPLM010000000
VERSION     DAZPLM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.287"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..591)
                     /locus_tag="ACHVC7_004058"
     CDS             complement(<1..591)
                     /locus_tag="ACHVC7_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR2117956.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(584..994)
                     /locus_tag="ACHVC7_004059"
     CDS             complement(584..994)
                     /locus_tag="ACHVC7_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR2117957.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(1000..1533)
                     /locus_tag="ACHVC7_004060"
     CDS             complement(1000..1533)
                     /locus_tag="ACHVC7_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR2117958.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1511..2587)
                     /locus_tag="ACHVC7_004061"
     CDS             complement(1511..2587)
                     /locus_tag="ACHVC7_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR2117959.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2584..3975)
                     /locus_tag="ACHVC7_004062"
     CDS             complement(2584..3975)
                     /locus_tag="ACHVC7_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR2117960.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
BASE COUNT          955 a         1205 c          988 g          924 t
ORIGIN      
        1 gcccccctgt ggaatgtagt aataacgttc cataacgcgg gcacgccata tttccagatt
       61 ttcaatatct tcaccgtctg taatggtgtc agcgtaccct gtggacggca gaccactgac
      121 gggtgttccc agttgcatgg caataccatc atccgtattc cccgccgatc ccggttcgtc
      181 tgccacaaca ggcacccgga gaagaccatc ggctgcggtc accgtcgccg tcgtggtaaa
      241 ggtcacctga ttatcacgct ggatctgcgt tcccgctggc aataccggcg tcccctctac
      301 gccatcccag cgcacaaatc cccgggctgc caccgcttct tttcgcgggc agcgtttgat
      361 tctggcgtgc cggtacagcc agtcttcatc acacacgtcc ggtaacagat ttctggcaag
      421 ataatcgata tacccgtaca acgtgtgtac ggctgcagcc tgtacacggg catacacttc
      481 cgcatccata cgacgaagca gcgtatcctg ctcaaagcgg gttaataaat cgctccggat
      541 catagaaata agttgcggga ggccggggcg ataaaactga ctgtcagcca ttcagttcac
      601 tccagatatc atcaaaaata atgttgtgaa tattgccgtc acgctggtaa atggtaatgg
      661 caagtgccag cgagtctgtc ccggtccgga cagcgttaat atcaagacgg gaagcaacac
      721 catcctccac catccacgcc agcgcctcac ggacataatc tctggcaagc tgcggggttt
      781 tatttgtcag cttgctgcgt cgcaacagat acaaacgcga ccccgtgcga tcattctgaa
      841 ccgcaggcca ggtatccccc caccatccaa atatccgtgg tgcatcatca cgcccggcac
      901 gccgccatgt aaaaagcgaa ataatcacag cacgcgtcag aaggtcgatc gaagccccgg
      961 tcgacacggg tcgcccgtta acattaatca tcatgatttt cagcccatcg gttgatccgg
     1021 cgtgtcagtg atcccgccac catcaccatt ttcggtatgt ttgtgggcat tgtaggcctg
     1081 ccgcatttgc tgcatgctga gtccgccact gtcgcaattg tcagtaatat cggcggtgga
     1141 ttcgactggc atttcaaagc gcgctttagg cgcgttttta aaaattatgg ccttcccggc
     1201 cccgtccacc acaataccgg aacgggtcag gacaacggac tgtcccagat cgtcataaag
     1261 cgccacttcc ccacgtttca gtcctttcag ccggtaacgt ctgtcagcca cgaccacaac
     1321 cacaccatga gaacgatcgc ccgccggaaa taaagcaacg ccctcagcac cgttctgtgc
     1381 ggcagatgta aaaccataag gttcaaggtg ctcaacgtgt tgtttctgat ctccggctat
     1441 cattttcaac cctacagact gacactttct ggcggaatcc accgccgtga tgacagcccg
     1501 ggaaatcaga ttacgaagag aaaacccgtt catcagaaat cctcctcaac atttttttct
     1561 tcctggcggt aacaggctcg gggagataag catctgccgg gccaacccgt aattcagtcg
     1621 tcgtgccccg gtcgtcctgg ttataggtaa cttctgcgat caccagttca tcattatcaa
     1681 aattattcag cgggtcgaaa acgatgacag ataaccccgg acgccataac gcgccactgc
     1741 cctgccgcca gccctgaacc gtatatgttg tctcacgggt aagcgcagcc cgttggcgcg
     1801 cttcaaattc acaacgggcc ttacaggttg ctgtcgttgc tgtgcctgac tgctgaatca
     1861 acaaagggcg ataacgggtc acgccactgt cctgaatggt ctgacgtatt gcggcaatgg
     1921 tggcctcacc aaaatcatcg tcgttgcccg ggcgctgccc actgacctga tattcagaaa
     1981 accgctctct gatgcttctt tccgtgtcac aggaaagaat attctctccc agcaccagcg
     2041 ccgtcgccgc tttacctgtt ccgggttttc ccagaaccag tcgcccgcat tcatcgtcat
     2101 aagccagcgt ctgaacctgc cccagtaacc ggttaagaca atcggcaacg gtttcgccgt
     2161 gttccggctg ggcatcaatc accgctgtct gcggcacgcc agcatcaaca acggtgatgc
     2221 caaatggcgc agccagttca ctgactattc tgagcaggtt ttttccgctc tgctggagtg
     2281 gcaaagcaga gcagtcaacc agatcggctg ttttgcttcg cccgacaatt cccatgctga
     2341 cgctgctggc gtcataacga agcggtagtg cctccacata tccggtgagc acaggctcat
     2401 ccccgataag cacttcaacc agctcaccat tttttatccg gggttgataa tcccggcttc
     2461 cgggccagcg ggtggtaatg gcaacattaa aatcccgggc aatacggtta atgcccgcac
     2521 tgatacggac ggatgtccag ccgccccatt cgcgaccgga aacccgaagt aaaacggtat
     2581 cattcatctg acgggtaccc ttaatgccct gaccggaaca aagcccggat gagagatggc
     2641 atttcgatcc aggatatcag tttcacggga cgcatcgtcg taccatgacg cagccagaac
     2701 aagcgcaggc agaacttccg ctggcgttcg ctctgcggtt tcctccgtct gaaccagacg
     2761 cgcctgaata tcccggttca gttctgtacg taatgacgtc agctgaaaaa acagcctgtc
     2821 atccgccgta cgcctgagct cctgttcaat cgccgcgttc agcgattcgc gtatgatggt
     2881 gagattttcc cgcgtgggtg gttgcgctct ctcatcctgt cctgtgctgg ctgccacgct
     2941 gtcaagtgcc ggatgagaaa cgtgaataat atcggactga cgttcagtgg aaccaccaac
     3001 agccacaacc gcctgctgat ttttcaccag gcttccgggt tgtggcagtg aagtgaccgc
     3061 ccttgtggct tcgctgattg ccgtcgtccg gatgacagca gccacaaggt ttgtctgctg
     3121 tttttgcctg ataaccgatg cggaatctgt gggccacacc gcacgcggtg ccagtcctgg
     3181 gtccagcgta atgccggaca ttgtcgttat ggactgcacc agatcctgtg tgttatccac
     3241 cagccgggtt ccggcccgcc aggtatcctg caacgtatgc acaaaatcac tggcaaccga
     3301 cggcggcatc agaatgacgg ataaatcgcc ctgcagtagt ctcgctccgg cagaaacagc
     3361 cgaattaacc attttgaaag ctgtctgaac ggttcccagc atatcggtcg tccgggcaat
     3421 aacatcgttc tgaataaagt ccggtattcc cgcgagatca aaatcgccga acatgtcttc
     3481 aatcaactca tccaggaata cggacgactc ctccagtttt ctggcggttg cagctccggc
     3541 gaccggaaat gacagttccc cgctctcaac aaactgaaat gaaacccggc acatacggcc
     3601 ttcagtgccg gagtgagaaa cggtcacctg cccgtcaata cagccctgca tttcaccgaa
     3661 ctgcggatgg atcagcgtcc ccggcccggc ggtttcaatc gctgtaatca gcctgtcgcg
     3721 ctgctctgcg taatcatcac caacgagata cgcattaatc gtcagccgtc gcgtggcacg
     3781 cccgagatct tccgtgtacg gtttgtcacg gtttgggtat tcatggacct gaacgcggcg
     3841 tccgaacgaa ccctcgtcgc tctccaccga aaacggaacg ccacgaaatg aagcgtcgta
     3901 aagattatcg cgccaattga tacctgaaga tgcagaaaag agtgaagaaa gagaaggtaa
     3961 agaaggtaag tccatactgt ctccactata aaaaaaccgc caatttctgg cggcttatat
     4021 attaatcatc attaatatct ttcatattca atttgcaatt ttcgtaggtc ac
//
LOCUS       DAZPLM010000286         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPLM010000286 DAZPLM010000000
VERSION     DAZPLM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.288"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHVC7_004063"
     CDS             <1..784
                     /locus_tag="ACHVC7_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR2117961.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHVC7_004064"
     CDS             781..1686
                     /locus_tag="ACHVC7_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR2117962.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHVC7_004065"
     CDS             1683..2753
                     /locus_tag="ACHVC7_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR2117963.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHVC7_004066"
     CDS             2990..3343
                     /locus_tag="ACHVC7_004066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117964.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            3469..4008
                     /locus_tag="ACHVC7_004067"
     CDS             3469..4008
                     /locus_tag="ACHVC7_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR2117965.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1101 g          983 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attataaaaa tattttttgt
     3421 ttcagatgtc atatatttta ttaactgaat gattaacagg agaatcagat gaactggcat
     3481 tatgaaaaaa atggtgtacg ttatgataac gtaacagagg aagatattat cgaacgtatc
     3541 cggcgtggtg aactgaatgc atcaacactg gtgtggcaac agggcatgct ggaatggcag
     3601 ccagtttcag ccacatcact ggcggaaata ctgaaacaaa gtgaagttcc cccagccctt
     3661 ccgggtcacc gcattcccgg aggcgttgtc tggacgctgg catttgcccc tttgattggg
     3721 tacatacttg agatgtggac tgcagggctg aacggtatgt catttgaacg ggcttatgat
     3781 gcagtcagcg aaggccagta ctggtttatc tctctgattc tgaatattgc tctgggatat
     3841 ctggatgaac gaaaactccg taaatctggt gttgatactg caacatttgg ctggctggca
     3901 tggttgatcc cggtttatct gtggcgtcgg gcaaaaatcc tcggacaaaa acctgcgtat
     3961 ttctgggtct ggttggttat gctgatactg gtgttattta cggtctgata ttctttatcg
     4021 cagagtttac taaataatca ggttattagt gttcttaaca g
//
LOCUS       DAZPLM010000287         4048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPLM010000287 DAZPLM010000000
VERSION     DAZPLM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.289"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHVC7_004068"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHVC7_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR2117966.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHVC7_004069"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHVC7_004069"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR2117967.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHVC7_004070"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHVC7_004070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR2117968.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHVC7_004071"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHVC7_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR2117969.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1142 c          972 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccga
//
LOCUS       DAZPLM010000288         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPLM010000288 DAZPLM010000000
VERSION     DAZPLM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.290"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="ACHVC7_004072"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="ACHVC7_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR2117970.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="ACHVC7_004073"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="ACHVC7_004073"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR2117971.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="ACHVC7_004074"
     CDS             2833..3813
                     /locus_tag="ACHVC7_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR2117972.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3923..4043
                     /locus_tag="ACHVC7_004075"
                     /pseudo
     CDS             3923..4043
                     /locus_tag="ACHVC7_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1219 a          845 c          915 g         1064 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttttta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc accgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc gga
//
LOCUS       DAZPLM010000289         4012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPLM010000289 DAZPLM010000000
VERSION     DAZPLM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.291"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHVC7_004076"
     CDS             complement(<1..356)
                     /locus_tag="ACHVC7_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2117973.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECR"
     gene            complement(371..1117)
                     /locus_tag="ACHVC7_004077"
     CDS             complement(371..1117)
                     /locus_tag="ACHVC7_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2117974.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1124..1432)
                     /locus_tag="ACHVC7_004078"
                     /pseudo
     CDS             complement(1124..1432)
                     /locus_tag="ACHVC7_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1811..2323)
                     /locus_tag="ACHVC7_004079"
                     /pseudo
     CDS             complement(1811..2323)
                     /locus_tag="ACHVC7_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2336..2758)
                     /locus_tag="ACHVC7_004080"
     CDS             complement(2336..2758)
                     /locus_tag="ACHVC7_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR2117975.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2755..2997)
                     /locus_tag="ACHVC7_004081"
     CDS             complement(2755..2997)
                     /locus_tag="ACHVC7_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2117976.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3094..3513
                     /locus_tag="ACHVC7_004082"
     CDS             3094..3513
                     /locus_tag="ACHVC7_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2117977.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3819..3974
                     /locus_tag="ACHVC7_004083"
     CDS             3819..3974
                     /locus_tag="ACHVC7_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2117978.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1007 a         1076 c          893 g         1036 t
ORIGIN      
        1 cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc tccgtctggt
       61 aatgaccaga taaggtcagg atcaaccaca accggtttct tcacctttac cctcgatagc
      121 tttttgcggg cgttttgcca gtctttatga gcctgttcag agggaaatac cccgtagcca
      181 gaattatata catcaccact tgcgaccagt tccatgcata agcgaccgac agacgcacga
      241 ctgatacctg tttcatctga caactgccga atcgtgaccc gaccgtcctg atgcacgagt
      301 tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt cgccataaat
      361 cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag ctctcccagt
      421 taaaattcac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc tcgccgagaa
      481 gtgtattcat cgctgcatgg ttaatatttg tcagcatccc cacaccgcgt aacgatgccg
      541 tccggcgatc aacaatctgg ttcagtacca cctgctcgtt tttcgtatcc cgctgcatgc
      601 caatttcatc caggaccagc aggtcaacac cacaaagctc ctgtaaaaat ttttcaccgg
      661 atttgccgtt gtcgtagctc tcatgcaaca cgctcatgac atcggacacg gtgacgataa
      721 tcacgctacg ccccttcgcc atcagccggt tgccaatcgc gtctgcaaga tgattttttc
      781 cggtgccggg cttaccgctg aacacaaaat tcgtacagcc tgtcatcagt tcgtcagcta
      841 tggatttcgc ctgactcagt gcatgtcgct gaccgtcgtt ctgcgcccgg taattcgcaa
      901 acgaacactt ccggtgcaac ggctggatgc cggagcggtt caggattttt tccacccgca
      961 actgacgatt caggcggttg atctcctcgc aacgtttctg gccttcagca agttgccact
     1021 cgcgccactc cgcaaccgtt ctgaatgggg cggttacatg tggtggggtc agtctgcgga
     1081 tacgctccag aacgcctcct gtcgcaatat ttttcatggt ctgttacccc ctgaagcctg
     1141 gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac gtctcaggcc
     1201 gaacaccttt cggcgcgaac agaccctggt attcattggc gatgctgtgt cgaatcacct
     1261 gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc ccgttagcgc
     1321 cctgctccgt tcgaatcggt tttcgcaatg cctgcctgaa ctggacccac tcatgccaga
     1381 gtgtttctgg caaccagtcg ggcagatcaa ctgacaacgg gtcaaatttt ttctggcgaa
     1441 cagccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca ctgcgccgca
     1501 agacacatat cccggtttca acagcaccga ttttgagcag tgatttctgt cgcggagaat
     1561 ttcggtcaag ctcgctccca caccggacac cgttcctgtc agtcacgacg atatcaatcc
     1621 gccctccgca accgtcacca cgctctggca ccggatactc acgacagaca tccaggccag
     1681 cagcctgcaa cgcaaccacc gcagaattac aaaattccgc tgcagtaccg ccagataatc
     1741 tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg aagggtttgg
     1801 gatgggttag atctgttttt agatctttat ctgtatcttt attagttgcc tttgtgttgg
     1861 catcatgttc gaacatcact ccaacatctg tttgaacacc tgttaaattt ctctcttgtt
     1921 ttgtttgaac atctgcttcc tttctgcttc ttctggcctg aacagatgct tttcctgcgg
     1981 ctgatttttt ggttaatttt tctctgacag atgccagatc ttcctcaatc cgaagatgca
     2041 tccattcgtc gccgttatca caaaaaaact cccgcaagga tggttcaaca tcaacccatc
     2101 gctcgttagt cagacgggca atttttgcca acctgttttt gggtattggc tttcctgttt
     2161 gccagtaatt gaacatcagc aacaaatacg cgccgtgctc ctctgctgac aaatgcatgg
     2221 tgtcagccag gtaatcagct atgtacagtt gcatgtatgg taatgcggcc ataattgccc
     2281 cgtatgatgc tgcccggttg cttagaataa gcacaaacag catggaaact tttgcttaat
     2341 gaacaatgac agaatcgtcg gaagacccgc cgccgctgaa atgcgctttc cggtaaacgg
     2401 cctggactgc atcatcatgc gcatcaattg ccgtactcaa cgcttcctgc gccgccagta
     2461 atgcacggcg ttccagggta tcgaagatgc agagtcggtg acgcagctcg cgcggaagaa
     2521 ttgccagaac cgcagggatc agtttctgaa ttttttccct ttgcgctttc gtttcacctt
     2581 tcaaccaacg gtgatagata ttctgctgat tgttccagtc cttgcctggt acaaggggca
     2641 attcgccgcc cccctggcgc agatattctt cagtaattgc gttagcgacc cacgcctgcc
     2701 ctttttcggc tgccagggct aacaacactg attcgatgtg ctcatgcctg attttcatga
     2761 atcaaccgct cctatgctgt tttcgctatg cttaccgtct ggggggaata catcgtcaag
     2821 tccacaatga gcgccaagcc gattaagggt agaaacaatt tttctgcact cctctagtcc
     2881 aggggtacga aaatttgctt cgtaatttgc cagtcggctt tgtatccacc ctaactgaac
     2941 agcaagttgt ctttgagaca gcccaagctg ttttcgatat gttgaaattt tgttcattga
     3001 aaacctccga tgacaatttt aaacacacct tgtgttatat agccaagctg ttttgtgttt
     3061 tatgtaaatc acgattcgtg atacaaggat gcaatggaaa aagaaaacga aaaaattgcc
     3121 gctagtaggc tcaatgacaa aattgcaatg cgtcttaaag agcgcaggca gaagcttggt
     3181 ttatctcaag gaaaacttgc tgaaatctgc ggatggacgc aatcgcgtat aggtaactat
     3241 gaggcgggca gcagaaatgt tggagtacat gacgctgtcg tattgggaaa ggcacttggc
     3301 atatctcctc ctgagctcct ctttggagaa caggaatctt ctgaattgtg gttaaatgaa
     3361 tcccaacgaa aacttcttga gttgtttaac cagctaccgg gctcagaaca acaacgaatg
     3421 attgagctat ttgaagtccg gctaaaagaa atcgatgagt atgtagaaaa atatttgaga
     3481 ggcaggatta aagataatcc cccaccggaa taatgatctt gctatcacag taatatgcca
     3541 atcagcccgc tatcagcggg cttttttgta gcatcatcat atgacactca ccataaaaca
     3601 cattgcgtgt tgacacaaga aaacaaattg tgtttaataa gcatatccaa acaacgcccc
     3661 accagagaac ggcaggacaa tacctcgagt tatccagtca ctgaacaggg ctaagtagcc
     3721 agcctgaggc atacgaacat gacggcagtt gttgattgat acaaagcgca gtagataaaa
     3781 cgttccgcca cccggcgtta aggggaaatg gggtcaacat gaatactatc gatcttggca
     3841 acaacgaatc tctggtatgt ggcgtgtttc ccaatcaaga cggcacattc accgccatga
     3901 cgtataccaa aagcaaaacg tttaaaaccg aagctggcgc acgtcgttgg ttgggaagac
     3961 attcaggtga gtaaaatgaa cgagacagaa ttaaaacacg ttatcgctct ac
//
LOCUS       DAZPLM010000290         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPLM010000290 DAZPLM010000000
VERSION     DAZPLM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.292"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHVC7_004084"
     CDS             30..968
                     /locus_tag="ACHVC7_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR2117979.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHVC7_004085"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHVC7_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHVC7_004086"
     CDS             2310..2603
                     /locus_tag="ACHVC7_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR2117980.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHVC7_004087"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHVC7_004087"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPLM010000291         3940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPLM010000291 DAZPLM010000000
VERSION     DAZPLM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.293"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..588
                     /gene="fimZ"
                     /locus_tag="ACHVC7_004088"
     CDS             <1..588
                     /gene="fimZ"
                     /locus_tag="ACHVC7_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR2117981.1"
                     /translation="RMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDL
                     PGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPV
                     QMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLL
                     LSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHVC7_004089"
     CDS             complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHVC7_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR2117982.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHVC7_004090"
     CDS             complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHVC7_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR2117983.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHVC7_004091"
                     /pseudo
     CDS             complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHVC7_004091"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1097 a          972 c          861 g         1010 t
ORIGIN      
        1 agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg
       61 gatgattacc gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg
      121 gatatagact tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag
      181 agcacagtga aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg
      241 atacaagctg gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat
      301 cccgttcaga tgatcctctc cggatacacg ttttttccta gcgaaacgct caactatata
      361 aaaagcaata aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg
      421 accatattac gttatctggt tagcggatta tctaataaag aaattgccga taagttatta
      481 cttagcaata aaacagttag tgcgcataaa tctaatattt atggcaaact aggtttgcat
      541 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
      601 ttgatcataa atatcgcatc cgctttctcc acacctggct gaacaccgct ggctaacgct
      661 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
      721 ctcgcctccc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt
      781 tgtgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
      841 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
      901 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
      961 ttatcaatat ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
     1021 atattaatcg ttcccaaagg gtcagctgcc catccactgg aactccacag taacccgcag
     1081 aaacagctaa agagtactct tctcattctc cgtacctcat tgataatcga cgcgtaaata
     1141 ccccagcgcg ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc
     1201 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
     1261 atcgttaggc gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
     1321 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
     1381 ctgcgcagca acattcgtac atttgatcgc aatgctcttg gtttgcggca tgacgccagc
     1441 tggtcgattc cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
     1501 ttcaacaatc tgcccggcat ttaattcgca gttttgcggc acttcaaccc ggccaccaaa
     1561 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     1621 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     1681 aaatatcaaa cgagaatcca tcaccccgaa cggaaggttt tgcgaaacgt taggatctgt
     1741 tcccatccga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     1801 catcgcacct aatagataat catttaattg catatattta taattatcga tagtttcctg
     1861 aacaataaag ttagtgacat aactacggta tgtataattc accagcgtgc cgggtggaca
     1921 aattgctgag acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg
     1981 gttattggtg ctattaaaga catttgagag atcatagtgt tcatcttctg ctacgccagt
     2041 actatttgca caaacagtag cccaggatat gtttgccaga cacaaacatg ccattgccaa
     2101 taataatctg cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac
     2161 tgccgaaata acagtgacgg cttgctgtaa gctttgcttt ggcaatacat agtgcgcaat
     2221 gcagcgtgag ttcgcgccat ctccccactg aaccagtaat tcaccagaca atggcgcacc
     2281 acttaaataa acttgcccgt catcacccac catactggtt attccggtac tgttttcccg
     2341 taccagtgat ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac
     2401 gcctatgcgg gtatcaaaat tggcacgaac caacgcgcct tgcgtcggca caacgctgct
     2461 aatatttttt tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt
     2521 ataccgataa accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc
     2581 agtttgattt tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc
     2641 ccctaaaggc tggctcagtg ttatgccatt ttcatgcccg accacaccgc cagacagctg
     2701 ccagttaacg tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt
     2761 ggcctgccag tttgccgtgg cgctgcccgt gtacccatta ttcgaggtat cgccctggct
     2821 tacgtgataa ctcaggttgt ggccttccag taaggtccca ccaacgcctg ccagccagct
     2881 attttgcccg ttgctgttac ggttagcgtt caaggaggca taagcgcggt cgagcgcatt
     2941 ttcccgggtg tagcgacgtt tggtcaaaac attgaaaggc actgaaacat taagtccgac
     3001 aatatgttcg ttatcgggga tccctacaga ttcattccac gaaaatgaga gtgaataact
     3061 gatgccaacc cagctgctgg tataccccac ctgataccag gtatctgaat ccgaagtatt
     3121 ccagtatttt tgatgggtac cagagatata aagcgagcca aagtcattaa gtgattgtga
     3181 aatatttaac tgcaaacggt ctttgcggct aaagcgtaaa ttgtggtaat tcacgattat
     3241 cggttcatcg cgatgctcac cgtcgtaatc gtaatcatat tcgtacccct ccattcgacg
     3301 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
     3361 aaaattggtg ccgaaggtgt acatcgattt cgcatagagg aagcgaatgg aatccccctc
     3421 atgacgactg tcgtcggcta actggctgcg cgcatgggtc acatccagcg acactgcgcc
     3481 ccagttcccg aggttgcgcc ccagccctaa taaaaaggcg gtgtaattgg cagataattg
     3541 cgtcccgccg taggcagtaa attcctgtgg taaaccaccg agtgccgtgc cctgaaaaaa
     3601 gaaaggtgat gattgctgac tattaccgct acgaaaatcg cccgccgtca ggtcaaattt
     3661 gaaacgccct tcgcgttgta aaatcggcac tgttgaatac ggaattgtgt aattctgcca
     3721 tcgcgctcgt cgatggtgac atccagatcg ccatttgaag atgtcgggtg caaatcagta
     3781 atttcaaaag caccggggga aacatagctt tgatagataa taaaaccatt ttgccgaatc
     3841 gttagctggg ccgccgtacg ggcaatccca cgtaccgttg gggcaaaccc ttgctggcta
     3901 tctggataca tattatcgga agaataaagc cgtacaccac
//
LOCUS       DAZPLM010000292         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPLM010000292 DAZPLM010000000
VERSION     DAZPLM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.294"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHVC7_004092"
     CDS             24..590
                     /locus_tag="ACHVC7_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR2117984.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHVC7_004093"
     CDS             594..1169
                     /locus_tag="ACHVC7_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR2117985.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHVC7_004094"
     CDS             complement(1211..2263)
                     /locus_tag="ACHVC7_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR2117986.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHVC7_004095"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHVC7_004095"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR2117987.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHVC7_004096"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHVC7_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZPLM010000293         3828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPLM010000293 DAZPLM010000000
VERSION     DAZPLM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.295"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHVC7_004097"
     CDS             <1..197
                     /locus_tag="ACHVC7_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117988.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            208..375
                     /locus_tag="ACHVC7_004098"
     CDS             208..375
                     /locus_tag="ACHVC7_004098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117989.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            372..716
                     /locus_tag="ACHVC7_004099"
     CDS             372..716
                     /locus_tag="ACHVC7_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117990.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1008..1163
                     /gene="hokD"
                     /locus_tag="ACHVC7_004100"
     CDS             1008..1163
                     /gene="hokD"
                     /locus_tag="ACHVC7_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR2117991.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1384..1672
                     /locus_tag="ACHVC7_004101"
                     /pseudo
     CDS             1384..1672
                     /locus_tag="ACHVC7_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1711..1989
                     /locus_tag="ACHVC7_004102"
     CDS             1711..1989
                     /locus_tag="ACHVC7_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2117992.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1991..3049
                     /locus_tag="ACHVC7_004103"
     CDS             1991..3049
                     /locus_tag="ACHVC7_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR2117993.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3050..3430
                     /locus_tag="ACHVC7_004104"
     CDS             3050..3430
                     /locus_tag="ACHVC7_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2117994.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3427..>3828
                     /locus_tag="ACHVC7_004105"
     CDS             3427..>3828
                     /locus_tag="ACHVC7_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2117995.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          853 c         1046 g          895 t
ORIGIN      
        1 catcaccgaa aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc
       61 tacgccgcat ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg
      121 ttgctgttca tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg
      181 aaagcactgc gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg
      241 ttcattaaaa acccgcttga aaacgggctt acccgtggtg aacaaatgga actggcacgg
      301 attgcgctgg catcgctgga agcagagccg gttgtgttct ggtttgaaaa atatcaagaa
      361 ggggctacgg catgacgact tttaccagag agcagttaat agctcacgca gaggagacta
      421 ttgaagcaca gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata
      481 agatggatat tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta
      541 aattgcatga atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg
      601 taaagcagtt aacctggcaa ctgagaaaag cgaagccaga ctgcaaatta ccggataagg
      661 cgatgagtta tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg
      721 gcctgaagca ttaacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat
      781 ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg
      841 tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt
      901 taacattacg cgtccggcaa gacgcttaac attaatctga ggctcaatct atgaacggca
      961 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     1021 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1081 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
     1141 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1201 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
     1261 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
     1321 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1381 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1441 gttgagaatg gcgctattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
     1501 aagagtggag ctgcgttaat tcattttgtg gatgggcttg tgacggatgg ttttatttcg
     1561 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
     1621 ttttctgctt ttgaagatga gtgaattcat ctacaattca gcgcagggct gaacccctgt
     1681 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
     1741 gataattcag ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat
     1801 tggtaccagc atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc
     1861 aggcgcggat gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc
     1921 gtcaggctcc cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc
     1981 tggaggtaat gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat
     2041 cgttaagcca ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc
     2101 ggaaccaaaa agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct
     2161 ggtggaagac aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg
     2221 tggtgctggt gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca
     2281 cggcgattat catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt
     2341 gtgctggcac tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc
     2401 tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca
     2461 ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga
     2521 cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc
     2581 tgtgtacagt gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca
     2641 gcgcacaaaa aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga
     2701 aaagacggtg gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca
     2761 gaaatcacaa cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat
     2821 gacgtgtggc aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat
     2881 gggaacaaag gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact
     2941 acatgccgga gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg
     3001 ttttttaatg cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg
     3061 catagaattt gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag
     3121 cacatatttt gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg
     3181 ccagcagcgg ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc
     3241 accggataag cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac
     3301 gcatgcgggg ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca
     3361 gccagtatct ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt
     3421 caaaaaatga aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact
     3481 gatgcatcgg cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag
     3541 ggcatgacac agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt
     3601 agtatgaatg acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt
     3661 gatcgcgtgg cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt
     3721 atggcttcgt atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc
     3781 tgctgtcacg gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAZPLM010000294         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPLM010000294 DAZPLM010000000
VERSION     DAZPLM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.296"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHVC7_004106"
     CDS             complement(<1..182)
                     /locus_tag="ACHVC7_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR2117996.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHVC7_004107"
     CDS             426..683
                     /locus_tag="ACHVC7_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR2117997.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHVC7_004108"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHVC7_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR2117998.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHVC7_004109"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHVC7_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR2117999.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHVC7_004110"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHVC7_004110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR2118000.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="ACHVC7_004111"
     CDS             complement(3308..>3734)
                     /locus_tag="ACHVC7_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR2118001.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAZPLM010000295         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPLM010000295 DAZPLM010000000
VERSION     DAZPLM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.297"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHVC7_004112"
     CDS             complement(89..652)
                     /locus_tag="ACHVC7_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR2118002.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHVC7_004113"
     CDS             complement(756..974)
                     /locus_tag="ACHVC7_004113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118003.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHVC7_004114"
     CDS             complement(1133..1519)
                     /locus_tag="ACHVC7_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR2118004.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHVC7_004115"
     CDS             complement(1509..1880)
                     /locus_tag="ACHVC7_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2118005.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHVC7_004116"
     CDS             complement(1880..2137)
                     /locus_tag="ACHVC7_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118006.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHVC7_004117"
     CDS             complement(2134..3525)
                     /locus_tag="ACHVC7_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR2118007.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHVC7_004118"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHVC7_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2118008.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZPLM010000296         3601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPLM010000296 DAZPLM010000000
VERSION     DAZPLM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.298"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHVC7_004119"
     CDS             <1..173
                     /locus_tag="ACHVC7_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR2118009.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHVC7_004120"
     CDS             complement(345..674)
                     /locus_tag="ACHVC7_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR2118010.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHVC7_004121"
     CDS             complement(679..864)
                     /locus_tag="ACHVC7_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR2118011.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHVC7_004122"
     CDS             complement(861..3500)
                     /locus_tag="ACHVC7_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR2118012.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          875 a          970 c          819 g          937 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 g
//
LOCUS       DAZPLM010000297         3570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPLM010000297 DAZPLM010000000
VERSION     DAZPLM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.299"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHVC7_004123"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHVC7_004123"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR2118013.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHVC7_004124"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHVC7_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR2118014.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHVC7_004125"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHVC7_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR2118015.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          913 a          897 c          911 g          849 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agctaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccgatgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgtggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aataaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa
//
LOCUS       DAZPLM010000298         3561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPLM010000298 DAZPLM010000000
VERSION     DAZPLM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.300"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..869
                     /locus_tag="ACHVC7_004126"
     CDS             <1..869
                     /locus_tag="ACHVC7_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2118016.1"
                     /translation="ITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(986..2329)
                     /gene="gdhA"
                     /locus_tag="ACHVC7_004127"
     CDS             complement(986..2329)
                     /gene="gdhA"
                     /locus_tag="ACHVC7_004127"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR2118017.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2565..2837
                     /locus_tag="ACHVC7_004128"
                     /pseudo
     CDS             2565..2837
                     /locus_tag="ACHVC7_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            complement(2803..3210)
                     /gene="nudG"
                     /locus_tag="ACHVC7_004129"
     CDS             complement(2803..3210)
                     /gene="nudG"
                     /locus_tag="ACHVC7_004129"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR2118018.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            3297..>3561
                     /locus_tag="ACHVC7_004130"
     CDS             3297..>3561
                     /locus_tag="ACHVC7_004130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR2118019.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          898 a          885 c          872 g          906 t
ORIGIN      
        1 cgataacatc caacggtttg aatgaaagag gcctgattac cgcgggatgc tcttttcttt
       61 atctattaat tatgatgggg cttattgttc gggccggttt taaagcaaaa aaagaacaac
      121 tgcattatta tcaggctaaa ggtattgagc cgctcagtat cgagaagtta caggcgctac
      181 aattgatcgc accttatcga ttctatcata agcaatggtc tgaaacgcta gagttctggc
      241 cgcgaaagcc tgaacctggt aaagatacct tccaatatca tgtgcttcct tttgactcga
      301 tcgatatcat cagtaaaaga cgcgagtctt tagagaatca atggggtatc gaagatagcg
      361 aaagttattg tgccttaatg gagcattttc tttctggcga ccatggagcc aataccttta
      421 aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt gttgaataaa tttgctgttt
      481 ttccctcaga ctatatctct gattgcgcta ataatcgctc cggtaaatcc tcggcgaagc
      541 taatatgggc ggcggaatta tcatggatga cctcgatatc aagcacagct tttcaaaacg
      601 ggacaattga agaagaactg gcctggcatt atataatgct tgcttctcga aaggcgcacg
      661 agttgttcga aagcgaagaa gattatcaaa aaaatagtct aatggggttt ctttactggc
      721 atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt tatcgttacg
      781 acaagcaatt ttgggagcac tacagtaaga aatgccgttg gcccataaga aatgttccct
      841 ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg gcgctgcgct
      901 tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatttgtag gcctgataag
      961 cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc gcatcggcaa
     1021 ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac
     1081 catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt
     1141 tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat ttccaggccc gacgttgcta
     1201 caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc tggaacagtt
     1261 cagtcgcttc gatggtggtc ggcatatttg ccccttcagc gacggcttta acgccattgg
     1321 cgataagctg atgcgcggcg tcaacatcca gttcgttctg ggtggcgcaa ggcagggcga
     1381 tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca aattctttgg
     1441 cgtaatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt gccagtttct
     1501 ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca gtgatcacac
     1561 gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg gagccagaaa
     1621 cggaaacgcg catcccttca aaacccatac cgtggcgttt taacattgct tctgtgaaat
     1681 aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat gaaaggccct
     1741 taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc gccataaagc
     1801 cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcggtatcc gcgcccagat
     1861 gacgataaag ctcggtcatc agcgcctggc aaaaacgcat cacttcacct tcgctttttc
     1921 ctttcggatc gaaatcgctg ccgcctttac caccgcccat cggcagggta gtcagggcat
     1981 ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga aaggttaact gacggatgga
     2041 agcgcatacc gcctttgtac gggccgatgg cagagctgaa ctgcacacgc catgcacggt
     2101 tgacctgtat ctggttgcga tcatcaaccc ataccacgcg aaactggatc acgcgctccg
     2161 gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt tcaagaaaag
     2221 gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga ttcgggtcgc
     2281 gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata gatataaaac
     2341 ccttatatat taatacgatt gtgcttatgt tgtttatgtc atgtgctttt gcagttttcg
     2401 actataccat caagaatgtg acggtaagca agaaaaaaat gacccgggaa agcaaattaa
     2461 taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt tgccacccgg
     2521 ctaccgttat cagttgcaga gacaataacg ttaaggagtt ccctgtgagt cgagcattgt
     2581 tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat cgtccggacg
     2641 tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg cagccctgta
     2701 ctcagtgctg cgtttatcag gatcaaaatt attctgaagg ggcggtaatt aaagccgaag
     2761 gaatactgct gtaatgccag cgggatgaca aaacccttag cactaatccg ctgctctggc
     2821 ggcgcgtaaa gccataaacg cctctaatag tggaatgtca gcaggggcca gcggatattg
     2881 cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct gtaacgtccc
     2941 gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa cttctcgctg
     3001 atggctggca acatactcac ccacagttgc ttcgatgccc aattcttcgt ttaactcacg
     3061 caccagtgcc tgctgctggc tttcatccag ctcgacttta ccatcggcaa actcccataa
     3121 tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat cacgttcaat
     3181 gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata agcgaaaaat
     3241 gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga gtgcctgtgc
     3301 tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg cgggttcttg
     3361 ataaaccggg cattacgcca gatggtttaa cattagtggg atttgccatc ggcgtgctgg
     3421 ctctgccgtt tctggcgctg ggctggtatc tggcggcgct agtcgtcatt ttgttgaaca
     3481 ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agagcttacc gacgcgggcg
     3541 gctttcttga tatttctctt g
//
LOCUS       DAZPLM010000299         3560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPLM010000299 DAZPLM010000000
VERSION     DAZPLM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.301"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHVC7_004131"
     CDS             129..1376
                     /locus_tag="ACHVC7_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR2118020.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHVC7_004132"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHVC7_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR2118021.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHVC7_004133"
     CDS             complement(2228..2572)
                     /locus_tag="ACHVC7_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118022.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHVC7_004134"
     CDS             2814..3416
                     /locus_tag="ACHVC7_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118023.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          887 a          715 c          816 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttaaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgtc
//
LOCUS       DAZPLM010000300         3555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPLM010000300 DAZPLM010000000
VERSION     DAZPLM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.302"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHVC7_004135"
     CDS             complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHVC7_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR2118024.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            656..1846
                     /locus_tag="ACHVC7_004136"
                     /pseudo
     CDS             656..1846
                     /locus_tag="ACHVC7_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2031..3218)
                     /locus_tag="ACHVC7_004137"
     CDS             complement(2031..3218)
                     /locus_tag="ACHVC7_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR2118025.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3218..>3555)
                     /locus_tag="ACHVC7_004138"
     CDS             complement(3218..>3555)
                     /locus_tag="ACHVC7_004138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118026.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          907 a          948 c          827 g          873 t
ORIGIN      
        1 acttaaggtg cgtccggtga accaatcgga cgcgccttta ataactataa ataagtgtct
       61 tggcagatac tatataaatt aacttagtga atgattatgc taatgtcatc aattaaataa
      121 atataatggc gttaaggctt cccagtaata taattaatac tctacttcca gagtagaata
      181 ttaaatttta tccgcgtggt gcatcagcac aaatttatcc cacaactgtt cttctgtctc
      241 gacatgcgcc ggatctttca caatagtatt ggggatcggg cacaccttct ggcaggttgg
      301 tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt agatatgatc
      361 tcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat taatacagcg
      421 tttagtaatt agtaaagaca tttcaatgga ttaccgttaa atcattttaa aatcagtaag
      481 ttgtatcgag tttgtatgct ttactgtcat taacttactg tatgttgatc cagtgtattt
      541 aaccttgata aactcagtcc agcaacacaa aaccgcaaca cattgcattt tgtcccgtag
      601 aaaagacttg tatgtgtgag cttgttttct gcgcctacgc agataaggat tgagaatgcc
      661 gcgcactgta acacataatc cggatagccc caataatgac gatgttttag ccgcatctga
      721 aaaatgggac gcctgtaaac ccccctatac cagcgcacac atgaaaatct gtgttgctgc
      781 cgccaaaatc atcctcgctg cttccggcgt ggctcgccgt tccaaatacg aaaaagagaa
      841 ctatctccgt atcgatttca gcaaagccgg taaggttaca ttttacgccg agtttccaaa
      901 aaagatgggc ctcaaaggta aaaagctcgg cgagtggccg gagctcgcta tccagctggc
      961 gcgcgaaaaa gcactaggta tggctgacgg tggactgcgg gcagagtccg tacatgcagc
     1021 gctggaaatg taccgggatg acctcaaagc caaagtagcc cggcagaagc tgagcccgga
     1081 cagtttcaca acctacgggg tgcgtatcga ccggattaaa gcaacgttcg gcgagcgtga
     1141 ggtgttcagc gacgtaacat acaatcggct ggtggaagtg ctggacgagt ggatcgccac
     1201 tcgctcgaac aataacgccc tggagttgtt tgccgagctc cgtcggttct ggaagttctg
     1261 cgcacctact ctttgcaacg gccgcaatgt tgccgccagt ctgccagatg attatgtttc
     1321 ctcccgcgta cagaaaccta cccccacacg gctttttacc gatattgaat caatcgcccg
     1381 actctggctc aatgttgctg cctgcacctc tgtacaccag aagaatgctg ttcgcttcat
     1441 gatcatcact ggtgttcgtc cgattaatgt ccataacctg cgctgggact acgtttacga
     1501 ggaggctggt gaaattgttt atccggaagg ggttatcggc atgcgagggg ctatgaaaac
     1561 acaaaaggct ttccgcctgc cgataacgcc tgagatccgg cggattatcg acgagcagaa
     1621 agcctggcgt gattcagttc ctgagtgcaa cagggattat gtatttttgc agccacgtga
     1681 tccaatgcag ccattttcaa aacgatcact ggataagctg gtgaaaacat acagcccgga
     1741 cggggctgta aaaggaataa aacatgatgg gactgttaaa gggaaagacg gtgcatttaa
     1801 tacgatgtgc cgtaaattcc ttaagagcaa tgttattgcc ttgatcgata ggaattaaaa
     1861 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctca
     1921 tatgaacgga accgacgatt aaactggatg gctctgattg attcagggta tgaatggcgg
     1981 ttttttgctc cgtttccctc aaaatggacg caactttccc tctgcggctc tcagccgcac
     2041 caccgcatcc gggccagcgg ctcatgcatc aggacctgct ctgccagacg gtagccccgc
     2101 ttcagccccg taaaacgcat ctgactcccg cacagcacgc acttcagcgg gtcaaccttc
     2161 agtaacctct gatacatccc tctccaggtg atttgcatcg ccgtttttct cactgtctcc
     2221 gttatgatgt acaccacttc ttccagtaac cgccgtttcg ccggactcaa aaaaccgtag
     2281 tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc catgaactca
     2341 tctccactca tcaacaggta ttcttcccgt tttgttcggt gactgttgta acgcagaccg
     2401 atttcatcct gaccggcata atgctccaga cgactcatcg gcaccggtgg ctttttcagg
     2461 taagagccaa agtacaccgc cacatggggg gcattatcca tcacccggga tacgttgaca
     2521 ttccagccac ggcggtaatg cgtgtccagg aagcgattcc attcccgttt actgcttcct
     2581 tctgctgcca gcgcatccgg catcaccagg tcagggtatt tccgtgacag caaccgtgtt
     2641 atccggtagc gccacatgct catcacctta cgggcgtaaa aatgaagatt tttccaggtg
     2701 tggcccgacg tcacaccacc ggcagttgtc gataaatgga tatgcggatg ccactgctgg
     2761 tcacgccccc atgtgtggat caccgtgaat atccccggct ccacatctgc ctgatggcag
     2821 atttccagta tcacatccgc tgcaatgcgg ctcatctctg tcagtaacca ccggttgtgg
     2881 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtcg
     2941 gggaccaggc tcagcagata ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg
     3001 caggagcggc ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa
     3061 cagcaccact gtgtataccc catcagtgtg gtcccgcacg ccatgatttt ggtcaccgac
     3121 tcaatcacca ccggacgtac tgccctttcc ggctgcttct ccagccagtt aagccagtgg
     3181 tttccctgct gaaagatatc ggcaaaacgg ggaagcatca gaagggcggg gcgactccgt
     3241 ccggccagtg aaccgtgcca cactccgggc agtacatacc gccggcgctg ataccggaaa
     3301 gaatggtcgc aaattcccgc tccgtgcagc gggcgatttc cggataccct tcgtcatcaa
     3361 cacgtacaaa ccagaagacc agctttttgt ttcccgcatc cacaaagaac ggaatattca
     3421 ggtctgcgca gcattcaacg gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt
     3481 cttcgtcaaa aaaatcatct tcgtgaagct tcacgacata gcggggaagt ttgcttcttt
     3541 gagaggcggg tttac
//
LOCUS       DAZPLM010000301         3503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPLM010000301 DAZPLM010000000
VERSION     DAZPLM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.303"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHVC7_004139"
     CDS             complement(<1..1255)
                     /locus_tag="ACHVC7_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR2118027.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHVC7_004140"
     CDS             complement(1412..1621)
                     /locus_tag="ACHVC7_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118028.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHVC7_004141"
     CDS             complement(1644..2354)
                     /locus_tag="ACHVC7_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2118029.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHVC7_004142"
     CDS             complement(2870..3193)
                     /locus_tag="ACHVC7_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118030.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYGDNILVTD
                     SKVVH"
BASE COUNT         1015 a          742 c          579 g         1167 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc catacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttc
//
LOCUS       DAZPLM010000302         3415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPLM010000302 DAZPLM010000000
VERSION     DAZPLM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.304"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            763..1449
                     /locus_tag="ACHVC7_004143"
                     /pseudo
     CDS             763..1449
                     /locus_tag="ACHVC7_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1782..2657
                     /locus_tag="ACHVC7_004144"
     CDS             1782..2657
                     /locus_tag="ACHVC7_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR2118031.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2665..>3415
                     /locus_tag="ACHVC7_004145"
     CDS             2665..>3415
                     /locus_tag="ACHVC7_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR2118032.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKM"
BASE COUNT          938 a          801 c          806 g          870 t
ORIGIN      
        1 ccagactaat tattaataat ccagttatct ttttattcat atcatctcct ttccattggt
       61 taatttatcg gaaattcatc cccactaact aaaacgatcc acacaataaa actaaatcta
      121 attgcaggca atcaattcca gtcgatatat catctgtatg gaaatcatcg ctcatcactc
      181 atcgcagcct gaaagcccag agcaacgggg caggtctgca tataagtggt tacacggcag
      241 aattccagag ggttctgtca ctaaattcag tcactgacaa atttgcatga tgttgaatag
      301 acgagatttt gtgtcgaaat agttgtttca gtaactgtca ctcaatcggt ggtttatgtg
      361 actatattcg caaaaactgg tcgcagaaaa gtcctgtttt cagcttggaa acccggtaat
      421 cagaataatg gataggggaa caaaaatctc tgtatagaac gctccattga gggagagaaa
      481 acgtgtcatt ttgacccgtt cggtattcag aacacacaaa accgatcgcg ggtcgctttc
      541 gtgcatagtt atgcaacgcg ctaaaaacga ttctgacgca ttttttatgt ttaccctgtt
      601 ttttcttgtc tttttgagtt ttttgcgtca gagagcgtct gagagggcac gtacaacggg
      661 agttatggta aatggatcgg gttttcggga aggttcgaca ggatttgccg ttgggtgtag
      721 tgtaagcgac tgaaaaacaa acgcccctga aatcatgtcc agatccggca gaaccagcct
      781 catgcgcagt tctggctggt ttttgacgtt gatcgcgagg gggcagcgat tgactggagc
      841 gaccggaacg ctcccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg tcatgctcac
      901 ctgctctacg cgctcaatat cgccgtgaga accgcgccgg acagctcggt taaggcgctg
      961 aaatacgccg ctgcgattga acgttcgctg tgtgagaaac tttgtgctga tgtgaactac
     1021 agcggactga tttgtaaaaa tccgttccac ctggaatggc aggtgatgga gtggcgagag
     1081 gaagcctata ccctcgatga actggctgat tatctcgatt tgagcgcctc agcgcgccgt
     1141 agcatcgata aacattacgg gatggggcga aactgccacc tgttcgaaat gacgcgcaaa
     1201 tgggcttaca gggcgattcg tcagggctgg cccgaattct cacagtggct tgatgccgtg
     1261 attcagcgtg tcgaaatgta caacgcatcg cttcccgttc cgctttcacc gcctgaatgt
     1321 cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc ggaaactttc
     1381 gcacagtatg tggctgatac gcacacatta acgtacgttt tcgttccatt ggccctcacc
     1441 ccccgataac gacccaggta gcgcgccgta ttccccccgc cggccgtttt cttcgacatg
     1501 tacatcaggc gcggaactca tcggggcgcg ggaatgaaat cccattcaga ctccggatag
     1561 attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat
     1621 ccgttttcca agcggacccc ataatatcgg ctggtaaaca tcactcgcta gtgcattctt
     1681 tttgttggta atgataatca tttacattca ttctttgatg ttttatcata attaatgttt
     1741 tgtcaataaa tatatggaaa catattgttt cagatgggaa tatgagaaag ttcatcctta
     1801 tttctatgat aacgttagct agtgttagtc tgggagcctg cagacagaat gtcaccataa
     1861 agcaggatgc gccagggcag aaagctttcc tgactgacgg gcagatttac gatttgcaca
     1921 gtggcaaaat tatttctagc agcgagcttt tggcggatct tgctaccgct cgacatctta
     1981 ttattggtga aaaacacgat aatgccgagc atcatcagat tgagctttgg cttattcaaa
     2041 atttgctcat ccagcgacca caggggagtg tgttgcttga gatgttgacg tcagagcaac
     2101 agccccgagt taaccaagtt aaatgttggc tgaaagataa tcctgtggtt cgagatagcc
     2161 gtgttcagga actacttaac tggcaaaaag ggtggtcctg ggagatgtat ggcgacatcg
     2221 tcatgcagct attacgtggg ccttacccgc tgctcaatgc caacattggc cgggagcaga
     2281 tgttggcact gtacaaaaaa aacgaatttc ctaaaggaaa aaaatccact gcacctgtgg
     2341 tacaagaagc tctgcgtgaa accatcatat ctatgcatga aggcaacctt gaaagccagc
     2401 aactgacgtc aatgctctct attcagcagc agcgggatcg ctacatggcc cgccagcttc
     2461 tgagtgcccc ggtaccgtcg ttgttgattg caggtggtta tcacgcgtca aaaagcatgg
     2521 gcgtaccgtt acacatggaa gatcttgcca ccggcaccca tccagtggtg ttgatgcttg
     2581 cagaaaaagg aatgaacatc acagtagatc atgctgacta cgtgtggttt gtagcgccag
     2641 atacaacaaa aaggtaaacc atggatgatg aatattctcc acttctcaca gtcggtaaaa
     2701 tggagcagct ggtttatctg ttctctactg cttcatggac tgattttcct tgcgtttatc
     2761 tggcgttttt ccgaagttca gcccgctatg tctcctgcac cggcgatcat gctgcaatgg
     2821 gcagaaatta tagaggctcc ttcttctcca ctttcgcttc cggtgggaat cgctcaacag
     2881 gagtccgcag tgacagaaga aaagcaacaa actgaggaca gacaacaacg tcctgtcact
     2941 gaagacagtg atgcaaccat tgaaataact aggaagaaaa agtcatccga tggagaaaag
     3001 aaaaagacac gtccacctcg taagataaag gctcaaacca gcgacagcaa ccccacagcc
     3061 gtttccagta atgctgcacc tcaggctctg gtcgaatcgt cccgtattgc tgcgcctttt
     3121 aacagtgact caacaaagcg agataattct gaggcatctt gggaaagcag agtaaaaggc
     3181 cacctaaacc gttataaacg ttaccctggc gatgccagaa aacgtgcacg aaccggcaca
     3241 gccgtcgtga cttttaccgt aaacacggaa ggcactattg tcagtagttt cttggaaatc
     3301 tcttcaggaa ccctttctct tgaccgcgaa gctattgctg ttttagaacg cgctcaacca
     3361 cttcccaaac caccgcctga aatattggag ggaggattat ttaaagtaaa aatgc
//
LOCUS       DAZPLM010000303         3392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPLM010000303 DAZPLM010000000
VERSION     DAZPLM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.305"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHVC7_004146"
     CDS             complement(<1..1361)
                     /locus_tag="ACHVC7_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR2118033.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHVC7_004147"
     CDS             complement(1426..1545)
                     /locus_tag="ACHVC7_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR2118034.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHVC7_004148"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHVC7_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>3392)
                     /locus_tag="ACHVC7_004149"
     CDS             complement(2272..>3392)
                     /locus_tag="ACHVC7_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR2118035.1"
                     /translation="WEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNEQTLF
                     DNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQVKGHY
                     GDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNGNCSE
                     RLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPEVKLS
                     GENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNYDIQY
                     VNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFD
                     DILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          934 a          767 c          615 g         1076 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca ga
//
LOCUS       DAZPLM010000304         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPLM010000304 DAZPLM010000000
VERSION     DAZPLM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.306"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHVC7_004150"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHVC7_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR2118036.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHVC7_004151"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHVC7_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR2118037.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENQLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHVC7_004152"
     CDS             complement(1250..2824)
                     /locus_tag="ACHVC7_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR2118038.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHVC7_004153"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHVC7_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          712 g          969 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagct ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZPLM010000305         3282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPLM010000305 DAZPLM010000000
VERSION     DAZPLM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.307"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHVC7_004154"
     CDS             <1..190
                     /locus_tag="ACHVC7_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118039.1"
                     /translation="EMKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSAC
                     VQYLINQYMILQGGGNEE"
     gene            180..482
                     /locus_tag="ACHVC7_004155"
     CDS             180..482
                     /locus_tag="ACHVC7_004155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118040.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            537..1091
                     /locus_tag="ACHVC7_004156"
     CDS             537..1091
                     /locus_tag="ACHVC7_004156"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2118041.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            1213..1674
                     /locus_tag="ACHVC7_004157"
     CDS             1213..1674
                     /locus_tag="ACHVC7_004157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118042.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            2138..2881
                     /locus_tag="ACHVC7_004158"
     CDS             2138..2881
                     /locus_tag="ACHVC7_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR2118043.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            2964..>3282
                     /locus_tag="ACHVC7_004159"
     CDS             2964..>3282
                     /locus_tag="ACHVC7_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118044.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKK"
BASE COUNT         1167 a          552 c          660 g          903 t
ORIGIN      
        1 agaaatgaaa aaagtaactt taaaggaacg caaacatcat gtagtaaccg cacgtcttaa
       61 tgacgatcaa gtacttgtac ttaaccagct aattagtagt gggaaggtac agaaagattc
      121 attatcagcc tgtgttcagt acttaattaa tcagtacatg attttacagg gcggtggtaa
      181 tgaagaatga ctttgattta gtacaatatt ttgatgatta cacaatagta ggggatatgg
      241 attatcacta tttctattgt gtgaagtatc aggaaggttc attagaagca cattatgaac
      301 gaattggtga tccagaagta ttaggtacta ttacatggaa tggaaattac ttaatcgacg
      361 gattaccaat ggtaatgttt gtactggctg ctgaagatga acataaggaa tgtttaaacc
      421 gtttgctaat cgtatgtgag aaggcgaggg atgaatatag gctaaatgct actggcctct
      481 aagtagtagc agacgcgatc acatggagtg acgcgataac aacaaggacg tttgatatga
      541 accctaaaat cacttggaga ttaattccaa attcaaaaca ttactacgta tctgaaaatg
      601 gcgatgttta caatgataaa acaaaacagt atatagctac gaaaaaccca tctaatggat
      661 acaaaaaagt ttctattaat ggcaaatcac tatatgtaca tgttttggtt tgtactttat
      721 tcaatggtgc taaacccatt ggaggaagat acactgtaga tcatatcgac cgcgataaaa
      781 tgaataacaa agcaaaaaat ttacgttggg taactcagaa ggttaataat tccaatagaa
      841 agcataaagg tactcgtact caaaagctta ctctaaagca atggttaatg attgttggag
      901 agtaccttaa aggacaatta tctcaatatg caatcactaa atggttttta aatcaaactg
      961 gtattaatat ttgccctcag aatataggta agttattcaa aggtactacc tattcaaaat
     1021 ggtacacaac atatcttacg aataagcaaa gacaaagaat acaaagccta accattcaac
     1081 gtaaaccata aaaaatgcca gtattccagt actggcatct attcaaaact caatggcaat
     1141 gaagccaaac ttattaaatg gcctgtaatc actacgggtc acgattaatt aatttataca
     1201 ggattattta tcatggcaaa aacctattca acttttgata acagaaacat aaaagcacta
     1261 attagccagt acaagctata taaagatatc ccatgcgaag acatcaagaa catcatgaag
     1321 gactcagtag ctaaacttaa agtgagagat aagattagtg tatatgatgt gtttgcagta
     1381 ttgagtaatg aagatgaatt acggaatgat ttactattca atactatcaa tgctattaaa
     1441 gtacaacaga ataagaaaca agtaaaagaa cgtatgatca ggtactatgg ggcagcgatc
     1501 acggatgcag caaaacgaat agagcagtat atgttggcac acccaaacgc actgccagca
     1561 ccagtatatg gacaaagaac actaacatat aaagagtttg gtaaactatt ggaactacaa
     1621 aaacaaggtg actttattga tgacatgatc gagtacttaa agggcttgcg ttaattgtaa
     1681 cttattacgg catattacgg catggatgta tgccgtaata cggcaaaaaa agcccctgaa
     1741 acccgctatt taccatcaat acggcaacta agtatagaga tacgaaatcc attccatttc
     1801 attccatgta tttctttctc tatcagtatt ccaagaaact gtgacttgcg tcacgaatac
     1861 tactcgtcgt gaactcctcg tagtattggt tagtaacgtt gcgtttttct cgccagtact
     1921 cgaaaacccg caaggcgtta ctactcgctt tgctcgatag atgttagtac agcaaatgaa
     1981 gtacctaaag tgaattggta agtacgacat gccccgccag ttccacaact ctcgctctgg
     2041 tcatttccac tactatgatg ctattggata gctacataaa gtactggatc aagtaggcat
     2101 tttttgtgta caaagaggga attaagagag ggataccatg aacagaaaac aattcatcca
     2161 gtcgcatggt gctacttgta gtaactggac ttggagttgg tcatttgtga accacgacaa
     2221 gaaaatggtt atctttggtg catgggatgt acaaaaagag caagaaagat cagtcattct
     2281 gagggagcgg tggaaaacca aagttgtgga gggaaaggaa agaaaaaatc ttggatatat
     2341 tcaagctatt gaacacattc aattgatagc tgaaggttat gagctttaca cgtttgatat
     2401 ggagcaggga cgaaacgatg agaatagaga tgtagctgtt atcaaacggt tcactccaaa
     2461 acttgaaaaa cgttaccttc ggaaagaagg aacagtttgg tatgcagact ttttacctaa
     2521 tccatttcca gatgaaatta cctcacctga aaactacgct gagggtgcaa aaaagcaagt
     2581 tacagtaaac tcctacgagc gtgatccaaa agcacgacag gcttgtattg accatcatgg
     2641 tacttcctgc gggtgttgtg agtttaactt tgagaaagtc tatggtgaac acgccaaggg
     2701 ctttatacac gttcatcata tcaaaccgct acacacagta ggcgaggatt acgaggtaaa
     2761 tccaattact gatatggttc cactttgtcc aaactgtcac gcaatggtac atcgcggtaa
     2821 cgaagtatta agtgtcgaaa gtttaaaagc actgttgttt aaagacagca ataaaatttg
     2881 aatttatttt agacttgata cgacatactt gaaaagttat gaatacaata tgctttaaat
     2941 tagacatgta agttgagaga aaaatgagca taacaacaat agcagctaca tataaagatg
     3001 tgttaactat tgctttttcg gcgttgtcat ttattattgc atgtacttcc ttatacttct
     3061 ccggtagggc agcatggcat gatagatcaa ggctaaaaat aacagctgac ctcgtctacg
     3121 agtcttttga tgaggaacct tataaaatcg aggtgatagt gatcaatgtt ggtaggcgtg
     3181 atgcagtttt ggaagggatg ctttgccatt atgaaaaagg cattaggagc cattcatctc
     3241 caaaagagag cattgttatt aaagaaaagc aaagaaaaaa at
//
LOCUS       DAZPLM010000306         3280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPLM010000306 DAZPLM010000000
VERSION     DAZPLM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.308"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHVC7_004160"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHVC7_004160"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR2118045.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHVC7_004161"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHVC7_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR2118046.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHVC7_004162"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHVC7_004162"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR2118047.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHVC7_004163"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHVC7_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR2118048.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          772 a          868 c          780 g          860 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt
//
LOCUS       DAZPLM010000307         3230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPLM010000307 DAZPLM010000000
VERSION     DAZPLM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.309"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHVC7_004164"
     CDS             <1..396
                     /locus_tag="ACHVC7_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2118049.1"
                     /translation="KEDDFLSLDELNDFLSMNESDLEVSSKKTSKQDREITDVSVDSD
                     HKNTQCPATDDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDY
                     YLSPKKKQTQDDKKQKIKPKMYFAGGGIK"
     gene            396..686
                     /locus_tag="ACHVC7_004165"
     CDS             396..686
                     /locus_tag="ACHVC7_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118050.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1007..1297
                     /locus_tag="ACHVC7_004166"
     CDS             1007..1297
                     /locus_tag="ACHVC7_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118051.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1297..1716
                     /locus_tag="ACHVC7_004167"
     CDS             1297..1716
                     /locus_tag="ACHVC7_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118052.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            1884..2207
                     /locus_tag="ACHVC7_004168"
     CDS             1884..2207
                     /locus_tag="ACHVC7_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118053.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2637..>3230
                     /locus_tag="ACHVC7_004169"
     CDS             2637..>3230
                     /locus_tag="ACHVC7_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118054.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1071 a          579 c          676 g          904 t
ORIGIN      
        1 aaagaagatg actttctttc tttggatgaa ttaaatgatt ttctctctat gaatgaatct
       61 gaccttgaag taagctctaa aaagacttct aaacaagaca gagaaatcac tgatgtgagt
      121 gttgatagtg accataaaaa cacacagtgc cccgccactg atgatgagtt ggaccccttc
      181 gatgatgaag aggatgaacg tgaaaaagcc attaagaggg cagagaagaa aaagagattc
      241 gagcttgcaa gacaaaagaa caaagtatcg ggtttagacc cattcgatga cagtgaagat
      301 gattattatc tttcaccgaa aaagaaacaa actcaggatg acaagaagca aaaaattaaa
      361 ccgaagatgt attttgccgg tggaggaatt aagtaatgaa agaatatcac ggtgaaaaga
      421 gatacaaaga ttacctgtta aagcgttaca gcatttctcg tgaaggtcat cttatgaagg
      481 atactcacgg ggaagtttac cggattagac caaagaaaga agggaggggt tatttctttt
      541 acgatggtgt gacagattta aagattgacg ctttgaggtt tgcagtgctg tatcattttg
      601 atatctggaa ttcaatccac cagctacgat taaaagacgg tgaccccagc aacttaaagg
      661 ccaccaatat tatcacaaaa cgttagtttg ttttaccatc atcaatcgta attgtgattt
      721 catatttgaa aagcttaatg ctgaaattga atcgtgtact tctggcgata atcattttta
      781 aaagctgata cacaagaagc accagaaaca caaagcaacc taagcttgcg aatctgtgag
      841 attcagcaat tgtttcaaga aagcccatta attcccctta tttatcaaag ggaatgacct
      901 gtatcagccc cctacaacca tattaacatg taaatgagtt gttgacaata tgttataaat
      961 atgttaaagt tagttataag tcaaagattt taagaaggaa aatttaatga gacacacagt
     1021 tacacgcctt ttgccagagg catattccag actggcagca atcaaagaca aatacccagc
     1081 catgagccac acagcacttg ttggtgagat tctgaaggaa cacacagccc ttacaccgtt
     1141 tgacgctatc aagggcgttg cagagggttc taaggccatc agaatcacaa cgggccaact
     1201 ggaactactc aaagcacagt ccgcagtggc aggttgctca atgattcagg tgttgagtgc
     1261 caaggttcat caactggcgg ataagctggg ggtttaatgc tatgcacatt tgattatcat
     1321 gcgttttatc gccatagagg tcaccttgtc cgtgagaata acggaacatg gaaagattta
     1381 actcgtaatg ctgaaccctt cacacaccag caattaaaat cccgcttctc cagatggaag
     1441 aatgcaccag cagatactgg tgattttgag attatccata ccttcgatta tgcctcagaa
     1501 gatgaccttc acgatgcaat taagctgatt gaagacttca atacctcaga agatgtggat
     1561 ggtgacaaat ataaatcccg tggtaaccgc tataaccgtg atgatatcac tcaggccata
     1621 aatgctggtc aatctcagcg atacattaca gaaaaatatg gtatttcacg gagacagtat
     1681 ttctacgtta agtcattgat taataaagac aattaaaaat aaccatctaa gaaacggtag
     1741 aatgcacttt acaggcagag ttgcacctta atttgataaa ttaccttacc agccagtgca
     1801 actccctctg taacccgcat ggttactggc ctcagaatgg ctattttaag aggtctgagg
     1861 tgtgtttcat aggagattta aaaatgaata attacccaat gcaaattatg gttgataacg
     1921 acactgctat gatggtgcaa agcttcattg atgcaggtat cgcaattaat tttgataaat
     1981 tactaaagct gatggctgat aatgcagaaa acatctctga ctttattcag tcggtggagt
     2041 ttaacgagcc tcgaatgatg ctaccaatta cagacagcaa catgaaaaga ttggttatcg
     2101 agcaaacgaa catatacagt atttccccag agcaattttt gaaaggtgct gtgacaatcc
     2161 tgtacagtga taatattctg gtgacggatt ctgtgagggt tcactaatgg gtttaataat
     2221 tgcttggacg ttgcttggct tatttgttgg ttgcattatt aacaatgcgg taaataagaa
     2281 ttgatgttat caacctgtta aaaagctgtt gacaaataac cttaagcatg gcataatttt
     2341 aattatggga gagcaattct ctcttatttt tctttcaata atccgatgga tggatattct
     2401 gagttggaaa ggtgcaatgc ttttcgtcat ggcctttcag gttagtctga gaggctatct
     2461 caaaaagtac tcttgttcat tcattattga atcctttgcc cttcacagaa atgtttaggg
     2521 ctttttcagt ttttaaaagc acacttggca ggttcttgtt cagtcagact cttcacctct
     2581 gattgctgag tgtgcttcta taaattgaaa taacaaataa actaaggggt gaacgaatga
     2641 ttagaaatga acatgaaatg cttatcgaac acagaccaag tgcacttgat attcacgaat
     2701 taactttgcg tgaatctttt gatagagctt ggcgggaatc acacgaagta aataaaggtc
     2761 catatattcc accagaacca ctcgagattc caagagttga aattgatttt tctatgaacg
     2821 aacggtgtga acttgatttg aagttgaagc tacaacgccg atatttccaa gctattaaag
     2881 actctcaaga agcaatgaga agtgtttacg agaaaattca gtccaagctt gaagacgaaa
     2941 ttgacgaaat ggttatttcg atggagcttc aaaccaattc ttatgcttgt ttctctcgac
     3001 tagaccgtga cggttggggg tatgaccctg tagagattgg taaagtgatt gcagatttac
     3061 cagaaggtca aagagttgtt cgtacagaaa gagctgtcag agcaccacat cgactgtctc
     3121 taattgttgc tgataaatca caagctgagc ttgaagaaat tgccaaagag aaactgatga
     3181 aagcaagaga aaatgagatt aatttgctga aatcaagact ggctaagctg
//
LOCUS       DAZPLM010000308         3218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPLM010000308 DAZPLM010000000
VERSION     DAZPLM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.311"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHVC7_004170"
     CDS             <1..495
                     /locus_tag="ACHVC7_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2118055.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHVC7_004171"
     CDS             525..3041
                     /locus_tag="ACHVC7_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2118056.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3218
                     /locus_tag="ACHVC7_004172"
     CDS             3052..>3218
                     /locus_tag="ACHVC7_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118057.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          778 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggagtt
//
LOCUS       DAZPLM010000309         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPLM010000309 DAZPLM010000000
VERSION     DAZPLM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.312"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHVC7_004173"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHVC7_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR2118058.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHVC7_004174"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHVC7_004174"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR2118059.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHVC7_004175"
     CDS             complement(1715..1984)
                     /locus_tag="ACHVC7_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR2118060.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHVC7_004176"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHVC7_004176"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR2118061.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHVC7_004177"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHVC7_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR2118062.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPLM010000310         3045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPLM010000310 DAZPLM010000000
VERSION     DAZPLM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.313"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHVC7_004178"
     CDS             <1..172
                     /locus_tag="ACHVC7_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118063.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHVC7_004179"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHVC7_004179"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR2118064.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHVC7_004180"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHVC7_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHVC7_004181"
     CDS             2190..>3045
                     /locus_tag="ACHVC7_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR2118065.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZPLM010000311         3028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPLM010000311 DAZPLM010000000
VERSION     DAZPLM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.314"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            146..859
                     /locus_tag="ACHVC7_004182"
     CDS             146..859
                     /locus_tag="ACHVC7_004182"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2118066.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            891..1649
                     /locus_tag="ACHVC7_004183"
     CDS             891..1649
                     /locus_tag="ACHVC7_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118067.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1695..>3028
                     /locus_tag="ACHVC7_004184"
     CDS             1695..>3028
                     /locus_tag="ACHVC7_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR2118068.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          683 a          734 c          813 g          798 t
ORIGIN      
        1 taaaaatatt tatttggtta atatgttttt atgaaagcgt aattcaggtc aatgtcacaa
       61 ttaaccattg tcacaataag gttgaacgga tattctgagc acggaccacg taatattcaa
      121 tacattattt actgccgggt ttttcgtgaa tcaacgtaat atgtcaataa ttaattccac
      181 gccagtgcgt gttattgcca ttgtaggatg tgatggttca ggtaaatcga ccctcacggc
      241 aagcctggta aatgaactgg cagcaagaat gccaacagaa cacatttatc tcgggcaatc
      301 gtccgggcta attggcgaat ggatttcaca gctccctgtt attggcgcac cttttgggcg
      361 ttatctgcga agtaaagcgg cacatgtgca cgaaaaaccc tcaacaccgc ctggcaatat
      421 tactgctctg gttatctatc tgctttcctg ctggcgggcg tacaagtttc gcaaaatgtt
      481 gtgtaaaagc cagcaaggct ttctgctcat caccgaccgc tacccgcagg ttgaagtgcc
      541 gggttttcgc tttgatggcc cgcaattggc aaaaaccacg ggcggtaacg gttggataaa
      601 aatgttaagg cagcgcgagc tgaagctgta ccaatggatg gcatcttatt tgcccgtatt
      661 gttgattcgt cttggcattg atgaacaaac cgcgtttgcg cgtaaacctg accaccaact
      721 ggcagcgtta caggaaaaaa tcgccgttac gccgcaactg actttcaacg gcgccaggat
      781 ccttgaactg gatggtcggc aaccagccga tgaaatcctg caagcgtcac tacgtgcaat
      841 tcacgccgcg ctttcctgat caccaaaatg gataggggat gatttaacga atggatgcac
      901 tacaaacaca aactgttaat agtacaaccg cgccgcagcc cagctacatt ccggggctaa
      961 ttgcggtggt cgggtgcgat ggcaccggta aatccacgct gaccaccgat ctggtgaaat
     1021 cattgcaaca gcactggcaa accgagcggc gctatctggg gctgctctcg ggcgaagacg
     1081 gcgacaaaat caaacgatta ccgttggttg gcgtctggct ggaacggcga ctggcggcca
     1141 aatcctcgaa aacccaaagc atgaaaacca aatctccggc gctatgggcg gcggtgatta
     1201 tgtactgctt ctcgctgcga agaatggcga atctacgcaa ggttcagcga ctggcgcaaa
     1261 gtggcgttct ggtggtcagc gatcgcttcc cgcaggctga aatttcgggc ttttattacg
     1321 acggaccggg gattggcgtc gaacgtgcga ccgggaaaat cagcatgttt ctggcgcagc
     1381 gcgaacggcg tttataccaa caaatggcgc aatatcgccc ggaattaatt attcgcctgg
     1441 gcattgatat tgagactgcc atctcccgca agcctgacca tgactatgcc gagctgcagg
     1501 acaaaatcgg tgtcatgtcg aagattggct ataacggcac aaaaattctt gagattgatt
     1561 ccagagcgcc ctacagcgaa gtgctggaac aggcacagaa ggcggtttct ctggttgcca
     1621 tcgtttctga ccgccgaagt ttaacttaag gctggcggga tactttttac tatcaacgat
     1681 aaggtcaggc tgaattggcg ggttttaaca tcaaacattg gtttgcagat ggcgcgtttc
     1741 gcaccattat tcgcaatagc gcctggttag gctccagtaa tgtcgtgagc gccttgctgg
     1801 ggctgttggc gctctcgtgt gccggtaaag ggatgacgcc cgccatgttt ggcgtactgg
     1861 tgattgtgca atcgtacgcc aagtcgatca gcgattttat taagtttcag acatggcagc
     1921 tggtggttca gtacggaacg ccagccttga ccaacaataa tccgcagcaa tttcgcaatg
     1981 tcgtctcatt ttccttctcg ctggatatcg tcagcggcgc ggtggcgatt gtcggcggca
     2041 ttgccttact gccattcctt tcccattcat taggtctgga tgaccaaagc ttttggctgg
     2101 cggcgctgta ttgcacgctc attccttcaa tggcttcctc cacgccgacc ggcattctgc
     2161 gtgcggtaga tcgcttcgat ttaattgctg tacagcaggc gacgaaacct tttctgcgcg
     2221 cagcggggag cgtcgtagcc tggtattttg actttggttt tgcgggtttt gttattgcct
     2281 ggtacgtgtc gaatctggtt ggcggcacca tgtactggtg gtttgcagcg cgcgaattac
     2341 gccgcagaaa tatccataat gccttcaaat tgaatctgtt tgagtctgcc cgacacatca
     2401 aaggcgcgtg gagttttgtc tggtcaacca acattgccca ctccatctgg tcggcgcgta
     2461 actcgtgcag cacagtgctg gtggggatcg tgttaggacc cgctgccgcc gggttattta
     2521 aaatcgccat gacattcttc gacgccgccg gaacgccagc gggtttgctg ggtaaaagtt
     2581 tttacccaga ggtgatgcgt ttagatccgc gcaccaccag accgtggttg ttaggtgtga
     2641 agtccggttt actggcgggc ggaatcggta ttctggtggc gctcgcggtg ttgatcgccg
     2701 gcaagccgct catttcgctg gtgtttggcg ttaagtatct cgaagcgtat gacctgattc
     2761 aggtgatgct gggcgcaatt gtgatttcga tgctgggttt cccgcaggag tcattgctat
     2821 tgatggcggg aaaacagcgc gcttttctcg tggcgcaaac catcgcctca atcggctaca
     2881 tcgtattgct gttcatgttc tgtcatctgt ttggtgtgct gggagcagcg tttgcttact
     2941 tcggtgggca atgtctggat gtggtgctct cgttaattcc aactttaaag gcattttttc
     3001 agcgtcattc cttgctttat aacgcagc
//
LOCUS       DAZPLM010000312         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPLM010000312 DAZPLM010000000
VERSION     DAZPLM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.315"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHVC7_004185"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHVC7_004185"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR2118069.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHVC7_004186"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHVC7_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR2118070.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHVC7_004187"
     CDS             2805..>2992
                     /locus_tag="ACHVC7_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR2118071.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPLM010000313         2945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPLM010000313 DAZPLM010000000
VERSION     DAZPLM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.316"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHVC7_004188"
     CDS             37..264
                     /locus_tag="ACHVC7_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR2118072.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHVC7_004189"
     CDS             complement(265..639)
                     /locus_tag="ACHVC7_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR2118073.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHVC7_004190"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHVC7_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHVC7_004191"
     CDS             complement(2099..2674)
                     /locus_tag="ACHVC7_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2118074.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          763 a          695 c          694 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttcaa
     2941 ggggg
//
LOCUS       DAZPLM010000314         2935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPLM010000314 DAZPLM010000000
VERSION     DAZPLM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.317"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHVC7_004192"
     CDS             complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHVC7_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR2118075.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1758..1833
                     /locus_tag="ACHVC7_004193"
     tRNA            1758..1833
                     /locus_tag="ACHVC7_004193"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Asn,seq:gtt)
     gene            complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHVC7_004194"
     CDS             complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHVC7_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR2118076.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          861 a          783 c          620 g          671 t
ORIGIN      
        1 tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc
       61 tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca
      121 caactggctt tttttcacac ttttgcggtt cggatcgagg gtatttccac agccaacgtc
      181 cagtaaccat tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca
      241 tccagacacc aaccacaccc cagccaagca tgatgcccag cacataaccg acgacaaccc
      301 ggcaacccca catgctcaac atagaaaccc acatggcgta acgggcatcg cgagcacctt
      361 taaatccagc gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagcc
      421 aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac gatgccataa
      481 ccccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg gaaagccaga
      541 acacatgccg caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg
      601 taatgatcgt agaagcagag ccgagcgcac ttccgggtaa gttgataaga gccgcaattg
      661 aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg aacatttggg
      721 ttaataaccg accactggta aataacactg attcgacact cgcgggaata ccaatcccca
      781 tgacttccca gataatgcta aaattcagcg gtttaaaata gctctttaac gaaatcctta
      841 gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag
      901 aaatggttaa acccagccct gccccgacaa atcccagtcc cggccaggag aaaaggccgt
      961 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcaatagcg
     1021 gtattttcgt attccctgca ccacgaagtg ccccgctacc aataagagtg atggcagctg
     1081 ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
     1141 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaata agcgttgcca
     1201 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     1261 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag
     1321 caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat
     1381 cttttcccag ccagctgacc agaaaagtgc tcagaacccc catcaacagg acacaggcat
     1441 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
     1501 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     1561 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag
     1621 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     1681 acggcaaaca gttggggaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
     1741 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     1801 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
     1861 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctgccgct
     1921 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
     1981 gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     2041 tatccagacg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     2101 cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     2161 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa
     2221 tcccctgtcg gtaagtgatt aacggccact tcgctattga ttccagcgtc aatggtgtaa
     2281 tttgcgttaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga
     2341 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     2401 cattttgcaa caacgtcgca atttcctgtg gcgtcccctg gattagctcg agccgaacct
     2461 ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     2521 gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     2581 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     2641 cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatcct
     2701 ccagttcccg aatatgacgg ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct
     2761 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     2821 cggtaaactc cgggcagttc agatttcccg ttattgttaa agtctaatgc ccggcataac
     2881 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaat
//
LOCUS       DAZPLM010000315         2919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPLM010000315 DAZPLM010000000
VERSION     DAZPLM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.318"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHVC7_004195"
     CDS             <1..495
                     /locus_tag="ACHVC7_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR2118077.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="ACHVC7_004196"
     CDS             495..1085
                     /locus_tag="ACHVC7_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR2118078.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1834..2112
                     /locus_tag="ACHVC7_004197"
                     /pseudo
     CDS             1834..2112
                     /locus_tag="ACHVC7_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2172..2660
                     /locus_tag="ACHVC7_004198"
     CDS             2172..2660
                     /locus_tag="ACHVC7_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2118079.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2684..>2919
                     /locus_tag="ACHVC7_004199"
     CDS             2684..>2919
                     /locus_tag="ACHVC7_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2118080.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          833 g          692 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattacac ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacgggactg
     2881 gcgcgacgag tactgtgttg gcgacattgc tgtatacga
//
LOCUS       DAZPLM010000316         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPLM010000316 DAZPLM010000000
VERSION     DAZPLM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.319"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHVC7_004200"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHVC7_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHVC7_004201"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHVC7_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR2118081.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHVC7_004202"
     CDS             2354..2758
                     /locus_tag="ACHVC7_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR2118082.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..>2900
                     /locus_tag="ACHVC7_004203"
     CDS             2811..>2900
                     /locus_tag="ACHVC7_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118083.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTI"
BASE COUNT          861 a          612 c          623 g          804 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc
//
LOCUS       DAZPLM010000317         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPLM010000317 DAZPLM010000000
VERSION     DAZPLM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.320"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHVC7_004204"
     CDS             complement(<1..1199)
                     /locus_tag="ACHVC7_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR2118084.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHVC7_004205"
     tRNA            1492..1586
                     /locus_tag="ACHVC7_004205"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHVC7_004206"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHVC7_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZPLM010000318         2878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPLM010000318 DAZPLM010000000
VERSION     DAZPLM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.321"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHVC7_004207"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHVC7_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR2118085.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHVC7_004208"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHVC7_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR2118086.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHVC7_004209"
     CDS             1650..2099
                     /locus_tag="ACHVC7_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2118087.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHVC7_004210"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHVC7_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR2118088.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHVC7_004211"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHVC7_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR2118089.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHVC7_004212"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHVC7_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR2118090.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZPLM010000319         2875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPLM010000319 DAZPLM010000000
VERSION     DAZPLM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.322"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            151..1317
                     /gene="nhaA"
                     /locus_tag="ACHVC7_004213"
     CDS             151..1317
                     /gene="nhaA"
                     /locus_tag="ACHVC7_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR2118091.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHVC7_004214"
     CDS             1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHVC7_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR2118092.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2320..>2875)
                     /locus_tag="ACHVC7_004215"
     CDS             complement(2320..>2875)
                     /locus_tag="ACHVC7_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR2118093.1"
                     /translation="DIFPGTPFPLLFEIEVSSCTGNNTNCNSVRFNYNLNTILQVNLM
                     TCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQ
                     ESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIR
                     PRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          698 a          631 c          711 g          835 t
ORIGIN      
        1 ccgtcaaaaa ctcatctcac cgctgatggc gcaaatcctt taattgctcg taaaaaacga
       61 gttattccta cactataatc tgattttaac gatgatttgt gcggggtaaa atagtaaaaa
      121 cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt
      181 gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat gatggccaac
      241 agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca gctccgggtt
      301 ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct gatggcggta
      361 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc
      421 ttacgccagg ccgcatttcc ggttatcgcc gctattggtg ggatgattgt tccggcatta
      481 ctctatctgg cctttaacta tgccgatccg attacccgcg aagggtgggc gatcccggcg
      541 gctactgaca ttgcctttgc acttggtgtg ctggcgctgt tgggaagtcg tgttccgtta
      601 gcactgaaga tctttttgat ggctctggct attatcgacg atcttggggc catcattatc
      661 atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc ggctgtagca
      721 attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctt
      781 gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac cctggcgggg
      841 gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga
      901 ctggagcatg tattgcaccc gtgggtggcg tatctgattt taccgctgtt tgcatttgct
      961 aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat tctgccattg
     1021 gggatcatcg ctggcttgct gattggcaaa ccgctgggga ttagtctgtt ctgctggttg
     1081 gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca aattatggcg
     1141 gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
     1201 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc
     1261 tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc agtttgacag
     1321 gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg agagaaatga
     1381 gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat aaagaaggtt
     1441 ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc ggacagattc
     1501 gagcgctgga agagcgcctg caaggcaaat tatttaaacg caagggacgt ggtctcgaac
     1561 ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc ttaagccagg
     1621 aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac gttggcgtgg
     1681 ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa cgccgcagtg gtagaaggcg
     1741 agcccattca tcttcgctgc ttcgaatcca cccacgaaat gctgctggag caattaagtc
     1801 agcataaact ggatatgatc atttctgact gtccgataga ctctacgcag caggaaggcc
     1861 tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat ccaccaccag
     1921 aaaaatcgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg cgacgttcaa
     1981 tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta gaaatcctcg
     2041 gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg
     2101 ttgccccaac gctttatgca tatgactttt atgccgataa aactgtcgta gaaattggtc
     2161 gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg attcagcacc
     2221 cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca gcggcgcgtt
     2281 aatcggcacc tcccccaaag ttaaggtggg ggagatagat tagttgtaca taaccacgat
     2341 tttgactcgg ctcattattt gcccgcttga gacattattt ccatatgtac gcgggcgaat
     2401 aaatagagga atctgattac ttccttcatg gggatgctga aaagagtagt aattgctttt
     2461 accatactgg atttcattcg taccatcatt ggagagttga aagcctactt gacccgcacc
     2521 gtcctcactg ttttctaact cattttttaa tatgctggtt cctgactcct gtatcgtcac
     2581 tggttcaaag taatattcaa catcactggg ttcaaaaact aattcacctt gctcgccctg
     2641 gatgcagtca aatcttatat gatgaaactg ataattttca ttattgatac gagacagtac
     2701 aaatcgtcca gtgtcgatgg attgatcttc aaaaccacag gtcattaaat ttacctgtaa
     2761 aatagtattt aaattataat taaagcggac gctattacaa ttagtattat tccctgtaca
     2821 tgaactcact tcaatttcga acaacaaagg aaaaggagtt cctggaaaaa tatct
//
LOCUS       DAZPLM010000320         2868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPLM010000320 DAZPLM010000000
VERSION     DAZPLM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.323"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(21..1475)
                     /gene="xylB"
                     /locus_tag="ACHVC7_004216"
     CDS             complement(21..1475)
                     /gene="xylB"
                     /locus_tag="ACHVC7_004216"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR2118094.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1547..>2868)
                     /gene="xylA"
                     /locus_tag="ACHVC7_004217"
     CDS             complement(1547..>2868)
                     /gene="xylA"
                     /locus_tag="ACHVC7_004217"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR2118095.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          684 a          837 c          669 g          678 t
ORIGIN      
        1 ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg
       61 aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt
      121 tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc
      181 gccagccttg ctgcgcccag tgctggcccc acatcccccc ccgtacggta atcgagctgc
      241 tgaccgctga tatccgccag catctgacgc cagtactcac tacgcgcccc gccaccaatc
      301 aacgtaacac tttgcggttt aatgccgcag gcatgcacga catccatgcc atctgccagc
      361 gcataaccca cgccttccag cactgctcgc gccagttcat tggggccatg ttgatgagtc
      421 aaaccaaaga aaaccccctt cgcctgggga ttattgtgtg gtgtacgctc gccggaaaga
      481 taaggcagaa accaaactgg ctcgacactt tcatcagcct gttgagctgc agcgattaaa
      541 gctgggacat tgcacaggcc ggttaatttc gcggcccaat ccagacacga tgctgcactc
      601 agcattacag acattaaatg ccaacgctgc ggtagcgcat ggcaaaagct gtgtacagcg
      661 ctttctggct tgcttaagaa cccttcgctg acagcaaaat agacccccga cgtccccagc
      721 gataacattg cctgattagc atcaaccatt cccacaccaa ctgcaccagc tgcattgtcg
      781 ccaccgcctg cgacaactgg caccgtcgcc ataccccacg ctttcgcaac ttcaggtaac
      841 aaagcaccag taatttcgct gccttcgtat aatgcgggca tctggtcacg agataagtcg
      901 caagcctgca gcatgacgtc actccagtca cgctttgcga catccagcca catggtgcca
      961 gctgcgtcag acatatcgct ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc
     1021 ggtaataata ctttgtcgat ttgacggaat atctccggct catgccgctg aacccatagc
     1081 aatttaggcg cagtaaatcc gggcatcatc aggttgccgg taatcactcg tgattgcgga
     1141 actctcgctt ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca caaaatggca
     1201 gggcgcaata cccgttgttg agcatccagt aaggttgctc catgcatctg gccggcaata
     1261 cccaatgctt taacgtcctg cagagaatgc tgatcgccca gagctttcat tgcgcgatca
     1321 gttgcctgcc accactgttc cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaacg
     1381 gtcagctttt ccgtttgcga atcaaccacc tcaccctgct cgttgagcaa aataactttt
     1441 acgcccgagg tgccaagatc tatcccgata tacatatcga tcgttcctta aaaaaatgcc
     1501 cggtatcgct accgataacc gggccaacgg actgcacagt tagccgttat ttgtcgaaca
     1561 gataatgatt taccagattt tccagttgct cctggcgacc actctgatgc accggagaca
     1621 aattatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct ttcaggattt
     1681 gctggcccaa ttcgctattc cagccggaat aacgctgcgc gatgcgttta tccagctcgc
     1741 catcttcaat catgcgcgct gcaattttca gcgccagtgc catcgtatcc atcgcgccga
     1801 tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg gcatcgaagt
     1861 tcagaccacc ggtggtgaaa ccgcctgctt tgagaatttc atacatcacc agcgcattct
     1921 cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg ccacggttgg
     1981 cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga tggaaagagt
     2041 gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt tccagaccaa
     2101 actgtttcag gaagccatag accgtcgcgg catcgtaatc atattgatgt ttagtcggtt
     2161 cttgcggttt cggttcgata agcaacgtgc cctggaagcc gattttatgt ttatgctcaa
     2221 ccaccatctg cataaagcgg cccagttgtt cacgctcctg acgcaagtcg gtatttaaca
     2281 gcgtttcgta accttcacga ccaccccaca ggacatagtt ttcaccgccc aatttatggg
     2341 ttgcttccat cgctgtaaca acttgcgttg ccgcccagct gaagacttca ggatctgggt
     2401 tcgtcgccgc acccgcgcca tagcgagggt ttgtaaagca gttagcggtt ccccacagca
     2461 gcttcacgcc gctctcttct tgcttggctg ccaggacatc aaccatttgc gcaaaattat
     2521 tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg aagcaataaa
     2581 atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg
     2641 ccagtgcctc accaggctgc tgccacggac gattaaacgc ccccacacca aacatgtccg
     2701 ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc tcttccatac
     2761 gcttacccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg tttgaggatt
     2821 ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgca
//
LOCUS       DAZPLM010000321         2835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPLM010000321 DAZPLM010000000
VERSION     DAZPLM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.324"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            188..772
                     /locus_tag="ACHVC7_004218"
     CDS             188..772
                     /locus_tag="ACHVC7_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118096.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            877..1617
                     /locus_tag="ACHVC7_004219"
     CDS             877..1617
                     /locus_tag="ACHVC7_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR2118097.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1652..2835
                     /gene="htrE"
                     /locus_tag="ACHVC7_004220"
                     /pseudo
     CDS             1652..2835
                     /gene="htrE"
                     /locus_tag="ACHVC7_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          817 a          591 c          605 g          822 t
ORIGIN      
        1 aagaaaattt aatttgttca aaagccctta aagtgcacaa taggcgtcct aatgaacaat
       61 ggttgtaaat tttggtaaca aatatcattg ctcattaata tctagcatct gcttataaca
      121 tttgtgtcat gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag
      181 gatttttatg tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc
      241 aggtactgct tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga
      301 taacacctgt gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac
      361 tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa
      421 acctttcagc atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa
      481 aatgaccttc ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc
      541 tattaacaac ccgtctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat
      601 caaacaggtt caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac
      661 aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc
      721 aactgctggt tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac
      781 caattaaatt gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt
      841 attccctgtt ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca
      901 acagctttat gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc
      961 atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa
     1021 aataaaggga ataacccgtt gcttgtccag agttggttag atactggcga taacaacgct
     1081 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
     1141 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
     1201 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
     1261 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
     1321 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
     1381 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
     1441 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
     1501 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
     1561 catttttacg ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca
     1621 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
     1681 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
     1741 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
     1801 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
     1861 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
     1921 aacgcacaag cctgtatcac acttaagaat ttattgcagt ttcatataaa ttctcccgat
     1981 ataaataacg aaaaagccgt tctgcttgcc agggatgaaa cactcggcaa ttgccttaat
     2041 ttgacggaaa ttatccctca ggcttctgtt cgttatgacg ttaacgagca acgtctggat
     2101 atagacgttc ctcaggcatg ggtaatgaaa aattaccaaa actatgttga tccatcgtta
     2161 tgggaaaatg gcattaatgc ggccatgttg tcatacaacc tcaacggata tcatagtgaa
     2221 actcctggtc gaagaaatga cagcatttat gctgctttta acggtgggat gaatttaggt
     2281 gcatggcgac tgcgtgcctc aggcaactac aactggatga ccgattctgg cagtaattat
     2341 gattttaaga atcggtatat acagcgtgat atcgcctcgc tgcgttctca actcattctt
     2401 ggtgagtctt atacaacggg cgaaaccttt gattctgtca gtatccgcgg cattcgttta
     2461 tacagcgaca gccgcatgtt gcctccgaca ttagcgagtt ttgcgcctat cattcatggc
     2521 gttgctaata ccaacgccaa agtcactatt acgcaaggtg gctataagat ttatgaaacg
     2581 actgtgccgc caggcgcttt cgtcattgat gatctgagtc cgtcaggtta cggcagcgat
     2641 cttattgtta ctgtcgaaga atccgatggc tcaaagcgga cattctcgca acctttttca
     2701 tcggtcgttc aaatgttacg ccctggcgtt ggacgttggg atattagcgg cggccaggtc
     2761 ttaaaagatg atattcagga tgagcctaat ttatttcagg ccagctacta ctacggtctg
     2821 aataactatc taacg
//
LOCUS       DAZPLM010000322         2796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPLM010000322 DAZPLM010000000
VERSION     DAZPLM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.325"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..422)
                     /locus_tag="ACHVC7_004221"
     CDS             complement(<1..422)
                     /locus_tag="ACHVC7_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118098.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSE"
     gene            complement(555..866)
                     /locus_tag="ACHVC7_004222"
     CDS             complement(555..866)
                     /locus_tag="ACHVC7_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118099.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            1059..1610
                     /locus_tag="ACHVC7_004223"
     CDS             1059..1610
                     /locus_tag="ACHVC7_004223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118100.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            2361..>2796
                     /locus_tag="ACHVC7_004224"
     CDS             2361..>2796
                     /locus_tag="ACHVC7_004224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118101.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLI"
BASE COUNT          831 a          522 c          594 g          849 t
ORIGIN      
        1 aattcgcttt ttaatgaggc taacttaatg tttaatagat cgataacttc tcgttcagtt
       61 ttaactaaat gtgcgaaatt ttttggttct atatctggtt cagtattgtt aaggttgcat
      121 atattttcat atgttttcaa catctccaac gaaaaaagat cgcaattagt ccagaactta
      181 gtaacttgat ttaaagcatt ttcctttatt ttagtttgaa accaattttt agcgttccat
      241 gctgcatata gggctgctcc agccataatg agattggcaa tagaactaac ccagtcacta
      301 accgatcccc actcgaaacc ttcagaacca acaaagagtg attttattag tactaaaatt
      361 acaacaacca gtaatactgc tacggtcgat aaaagaacga tatttaattt tctacaatcc
      421 atcgaaagta gttcctgaac gcattaaagg ggcaaatact ttaccatccg aaacacaatc
      481 aagtacacta cccgttcacg tactgctcac atgttgggtt tgttattttg ttggggtgag
      541 cagtacgtgt atcctcagaa gggtagttcc ccctgatcac agaaccaatc accttcttca
      601 ctgctttgat tcatccggta ctcagcagat agatatgcta acccatcaat aacacttatt
      661 agtggtgttt cacaggtaag tacaaaacaa tctctctcta tagtacctaa ccagtattcc
      721 ctgtactgat tctggaagaa taccctatcc ccataatgaa attctgttag tgcagatccc
      781 caataacaca cggttgccca gcgaccgtta tgtgagaact tctgaacccc tcgcgtaatg
      841 gatggatcat agccgtcttt gctcatttgt taccctcctc tatgtattac tgtatatatg
      901 tacagtattg cagtcagttt cccaaaaatc cagcacccct ttgtttacca ctttgaatga
      961 ttaattctat tgtaatgatc aataaaattg atcgatttaa ttgccaatgt tttaaaaatg
     1021 atcaattatc aataacttaa agcgtaacga gcaaagttat gaacattgat aattgggcaa
     1081 taccgataac aagtttgggt ggtcagttgc tgggggttga gttggtcagc catgtagaac
     1141 gcgagggaat caggcagatc ggcctatggg aagaagaagt acttcatggg caactaatgc
     1201 ttattgaaag caaggcggca tggttcaggg ataacggttt gtattgtgtg attactactg
     1261 gccgtggtga accatacgag tacttaccat tccttcggca gatgacaagg gatatgaaag
     1321 tacataacca gcagtggctt gatgatctcg gtgtacatgg tggttctgct attcctctgg
     1381 ttacaggtga tttcgaggtt gcccgattaa atagaaggta cacagaagag aacattgacc
     1441 gtctgatctt tccagtactc ataaaaagca ttcgccagta ctgcgagaag gtgatcgtac
     1501 ccgtcagaga taaaacgtat tacccaattc tcagagaggc cggtgtatgg gcggtacagg
     1561 gagagttcag gccaatgcgt tttgagcaat gtgaaaaatt aataggataa caataatgaa
     1621 aaaattacta attgggttac tgattgccct tagtactgga tgtactacta cgcacagctt
     1681 catggataaa gcttacgttg gctatgtaga tgacaaggcc gtagtactgt ttgatgcagg
     1741 aacacgtaat ggggtgaagt actaccgtcc tcagttccag agtgattgcg gtcgcatcta
     1801 taacaagatc tatcatgatg acaatacaag tactggatgc ctcatacgag attcccgcat
     1861 tgctgaacta catccctata gagagcatgt aagtaaggta gtaccacatc agaccgatag
     1921 tgttcacgat tggttagaag gggtcaattc accatcccat atcctcgaca atctataggg
     1981 tacgtatggg aaaggtaatg gtgattttgt tagtactggc tggggtactc ttcaacattg
     2041 caggtgagga tgcaccccag ccacagtaca cggacaatga gaaccagaac gctatggatt
     2101 tcgcacagtc actaatcaag ggtaagtacc catgcgatat ggtgactatg gatagtactg
     2161 agaatggtac aggacgtatt gatgtacggt gtgatggtaa gtaccagtat gagatcatcc
     2221 ggcatgatgg acaatggcag gtagtatcga agtaatccga aaagtttagt gcttaggctg
     2281 tatggtgaga atctacaggg ctttttcttt atgcggtgat aagatcggag tgaacttttt
     2341 atgaaggaat agttagtaga atgaaaatta cagtcgataa tatctctgaa atgtttgcag
     2401 gaactaaaga actactaatc ggtggcttaa taacaggtat cgtcggagta cttattttaa
     2461 ctcgtgttag tggttatgaa tacgttaaag tacctttaat gttagtaatg atattcttac
     2521 taactgtagt tcctggtttt attgctatgc ttttttcacc actaacgaaa gtatttgcaa
     2581 agatatttga tattaaaagc agtaatatac tttgccgcat tttctcattt ctatatttca
     2641 tcttgtatat ggctattact ttcttggtga ctaattttat attcactacc gcaatgtcat
     2701 tacacggcgg ggatggtggg ttgagagcat gttttgcaat tggctgtttt gtcgttgcag
     2761 ttgagcagac aagaaaattc tatagtttgc ttatca
//
LOCUS       DAZPLM010000323         2749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPLM010000323 DAZPLM010000000
VERSION     DAZPLM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.326"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..539
                     /locus_tag="ACHVC7_004225"
     CDS             <1..539
                     /locus_tag="ACHVC7_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2118102.1"
                     /translation="YIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLT
                     TAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGW
                     IYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLA
                     AKQHYKNAERLLAGLPAH"
     gene            complement(549..1976)
                     /locus_tag="ACHVC7_004226"
     CDS             complement(549..1976)
                     /locus_tag="ACHVC7_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR2118103.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1973..2680)
                     /locus_tag="ACHVC7_004227"
     CDS             complement(1973..2680)
                     /locus_tag="ACHVC7_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2118104.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          779 a          660 c          611 g          699 t
ORIGIN      
        1 tgtatatatt cgcaattttt atagttgcag caattacctg tattagccaa cccaagaaaa
       61 cgacgttgcg tgataaagcc atggtgaatt atgcctttga ttatttaaga tcaccaggca
      121 gtctaccgtt aaccacggca gccacggagc tttccgcgat tcatggtcac tcaacatcgc
      181 aatatcgcct tggcgaattt tatcttcatg gtagcgacgg taaaccactg gattatacac
      241 aggcgagata ctggtatgag caatcagcag aacaggaaaa tccgcgcgca caaagcaaac
      301 tggggtggat ctacctgaaa ggcctgggag tcaaacccga tacccgtaaa gcaattctct
      361 ggtataagga agcagctgaa caagggtatg ctcatgctca atatactttg ggtttgatct
      421 acagaaatgg cactggtatt aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg
      481 ccgccaaaca acattacaaa aatgcggaaa gattacttgc cggacttccc gcacattaac
      541 gtcgactgtc acccgaagag atggagtatt tttcctatca aaccggcaaa gataaaaatg
      601 tagaaaataa ttttgatgac gcccagacag cccagcccgc catcgtcaac gggcattggc
      661 tgcggttgcg gctccggctc gacggtttcc tgaccggtaa aattcgccgc ctgaattaat
      721 aatcgacgaa taattaatgc ttccagtgga caacgatctt ctgcatcaag gcggctcaca
      781 atcggcatta atgattcttc tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa
      841 tataatggcc ataagggatg ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct
      901 ttgtgcaaaa ccatctcgac cagtgaacgc cccagcgcag agagtttttc atcgctgagt
      961 atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag tagctgttta
     1021 ccctgatcgt taaccagttc acgagcttca atcatcatca ccactaacgg gtaataatcg
     1081 acccgatgct gacggcacca ttgcaatatt aaagcatccg cctgggtgga gggatactcg
     1141 cgccagtagt cacaaagttc tgccagcaag ctttcgcctt ctccacaata gacgcgttgc
     1201 gcgtattcat aataacgcgc atcttcatta tcaggttcag cttcccgcca cgaccgggcg
     1261 taatccagaa gttcggggat atccaggcga cctgcgctat accagcggag taaatttctg
     1321 tgcagttttg catcatcggg gatgatcact gcgcgatgtt gcgctaaata caatgtcagc
     1381 cattctggat gcgatgacca aatccgagcg aacatttggt aaaaatcgat ggtgtcattt
     1441 tgcacggcaa cgggcagatg cagaatggtc tggaagttaa aaacattccc cttcgcaatg
     1501 gcatataaaa aggattccag ttcttcttcg tctatttccc cgacgttttc ctcctgcaac
     1561 caccgcaatc tttctgctaa taacaccacg cactcgaagg ataaatgggc agtgttcatg
     1621 gcgattgtgc acagcgacca gcgtaattca tcaatctctt ccatcgagca ataatttaat
     1681 tgctgaatga atcgctgcca ggcggagggc agaaaacgtt cgctatcaga ggcaagtaac
     1741 gcgcgaaagg cgagcagaat ttcatgttct gctacctcat attcttctgg ttgccaaaca
     1801 gatttagcag gcgactgcgc aatccggagc gcctcctcat acgcctgacg aagttgttta
     1861 aaaccctgcg gatcggtttc tggatgaaag gacggtaaca gcgccagata agcgcggcgg
     1921 ataatatcga cgtcagtcgt ttcctctatt tcgaggatct tccagcaatt tttcataggt
     1981 cgctcatatc cttaagcgtt tccgggcatg tggggtagtc agccagtggt tgccaggaca
     2041 aggcagggta atacgcatca tcactggcaa ataattgata aaacatatca ttgccggaat
     2101 cgtcgtattc tttacgagct aagaattcgc gtaataaagg cagatgatca tcttcgggag
     2161 actgccaata ttgccgacca aaggtataac cggcaaagta ttgccgccag ttactataat
     2221 aacgtagagc ccgtagatga atacgcgact ggatccatag actttcttct tcactcaacc
     2281 agttattaag tacgcccatt cgacttaaaa aacccatccg gacataatcc catgctttaa
     2341 ttcctccctc accacagcat tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt
     2401 gctcatttag ttcagcaaga tagtcacgcc attcacatgg cgaataacga aaccagcggt
     2461 gataaaaccc tgccaaccga gcggcatgac cgttatcgat cattcggtga attgtgcgca
     2521 gcaggtcatc acggtcatca attccccatg aggaagaaag gtcaaaaaag catgtaccta
     2581 aatacatttt gggggatgaa tgcgaaggta aacagcattc attgcgtatt tccatcggag
     2641 tggaaagtgc aaacaatatt ttttgcgacc ccatgtccat ataatttatc cttaaacatt
     2701 catttaatga atgtaaacta catttcgaga ctcagttatt tatactttt
//
LOCUS       DAZPLM010000324         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPLM010000324 DAZPLM010000000
VERSION     DAZPLM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.327"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHVC7_004228"
     CDS             complement(423..680)
                     /locus_tag="ACHVC7_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118105.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHVC7_004229"
     CDS             complement(677..1174)
                     /locus_tag="ACHVC7_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR2118106.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHVC7_004230"
     CDS             complement(1376..1834)
                     /locus_tag="ACHVC7_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2118107.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHVC7_004231"
     CDS             complement(1831..2328)
                     /locus_tag="ACHVC7_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR2118108.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHVC7_004232"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHVC7_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR2118109.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZPLM010000325         2703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPLM010000325 DAZPLM010000000
VERSION     DAZPLM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.328"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHVC7_004233"
     CDS             <1..247
                     /locus_tag="ACHVC7_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR2118110.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(287..1495)
                     /locus_tag="ACHVC7_004234"
     CDS             complement(287..1495)
                     /locus_tag="ACHVC7_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2118111.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHVC7_004235"
                     /pseudo
     CDS             1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHVC7_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2022..2690)
                     /locus_tag="ACHVC7_004236"
                     /pseudo
     CDS             complement(2022..2690)
                     /locus_tag="ACHVC7_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          747 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgcg acgcggcggt
      121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg
      181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgctg acagaaaccg
      241 atattgacat cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct
      301 gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg
      361 caagaacggc gtgccccgcc gctaaaatgt tacgagcacc atttacatcg gcgttcgctg
      421 tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttctct ttcgctgtat
      481 gaccacagta cgcgcaacgc tggcttgtat acgctggcgg aacagcaagc acctgaccgc
      541 cacgccagag ctgcttgtac tcaagctggc ggtgcatttc ataccagccc tgatccagta
      601 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
      661 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
      721 tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat
      781 gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc
      841 ttaactgtcg ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct
      901 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
      961 ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc
     1021 acgtaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat
     1081 tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct
     1141 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
     1201 cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
     1261 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
     1321 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
     1381 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
     1441 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc
     1501 aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt
     1561 tcctgatgca tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg
     1621 acagaaaaac tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt
     1681 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg
     1741 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
     1801 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
     1861 agtagctgcg gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
     1921 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
     1981 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
     2041 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
     2101 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
     2161 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
     2221 tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
     2281 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     2341 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     2401 gtatagttca aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc
     2461 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     2521 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     2581 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     2641 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     2701 ctg
//
LOCUS       DAZPLM010000326         2679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPLM010000326 DAZPLM010000000
VERSION     DAZPLM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.329"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..355)
                     /locus_tag="ACHVC7_004237"
     CDS             complement(<1..355)
                     /locus_tag="ACHVC7_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2118112.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(355..849)
                     /locus_tag="ACHVC7_004238"
     CDS             complement(355..849)
                     /locus_tag="ACHVC7_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR2118113.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1207..1383
                     /locus_tag="ACHVC7_004239"
     CDS             1207..1383
                     /locus_tag="ACHVC7_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118114.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1525..1677)
                     /locus_tag="ACHVC7_004240"
     CDS             complement(1525..1677)
                     /locus_tag="ACHVC7_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118115.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1665..2132)
                     /locus_tag="ACHVC7_004241"
     CDS             complement(1665..2132)
                     /locus_tag="ACHVC7_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2118116.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(2129..2626)
                     /gene="rrrD"
                     /locus_tag="ACHVC7_004242"
     CDS             complement(2129..2626)
                     /gene="rrrD"
                     /locus_tag="ACHVC7_004242"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR2118117.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT          655 a          724 c          626 g          674 t
ORIGIN      
        1 tggcgagtcg ttttttggag tgttcgcggg ctttttcctc cgtcatctga ataatcagca
       61 tatcagcagg atcgcaaatc acgttgtaaa tcacccagcc gtcaatcagg ccgatagtct
      121 tgccggttcg tgccgggcca acaaatatca ctgcgtcgta ttcacgcgag gccaggcagt
      181 tcatcggctc aataacatac ggtgccacca gcggatccca cgggactgag ttccctgccc
      241 ccatgggcac ccgcatatac tgagcaacgg catcagcaac ccgcattcgt ctcggtgcgc
      301 gaaggatata acctgaatcg gttcgtgctg cctttgcggt ttcctgattc agcattactc
      361 ctcctgctgt aattcctcct catcatccgc acctgcttca gtcacccgca gggctatctg
      421 atcgcgcaga tcatcaataa tggactgaac acggctcaca gcggcaggct gcagaccgca
      481 gtcacgttca agaatatccg gtaatgtctc cagcacctgc acgaccgctt ttgcccagat
      541 ggcaaactcc cgtctgacat cactggccgg aatgagttgt gccgtttcct gttcgaactt
      601 aagacgctca cgttcagact gataccaggc tttgcgctca tgcgcgtcca tttcgccttc
      661 tgcaaccggc ggtggtaatg ccagaaatgc cgacacaata tcaaccaccc gataaagctt
      721 gaggttgctt tcatgccccc ctgcaacggg tagattttgc agccttgccg cagcagtctg
      781 gcgatgtaca cctgacagtg ccgccagttg actgatattc agcgtcagat tttttaactc
      841 tcgatccata cccgctccag aatgttttaa acatgcatct tgcgaacaac tttaggcaaa
      901 cggtgttagt gatgaacaaa aaacaatcaa aatcgacacc ataaaaataa aaccactgta
      961 atatcaatcc attacagtag tggtgatgac gaatgaaatt tcaaaaacta gcctttttcc
     1021 gcgccgctcc cgccccgtgg caggccaccc caccgggagg acccgtcagc ctgacagcca
     1081 tgacgaacgt ctgatacagc gccctgcatg aatgacatcg ggataatcca gaaaggaata
     1141 gcatcgtacc cacaagaatc agtgtaagtg tcctgtttct tccacccccg cacaggactg
     1201 gcgagcatga gggacaaacc cgcgaaccat aaacgcggta aaaacccggt gtgcatcgtt
     1261 tttgattatt cccgcacact cgcgcagaag gagttccccg tcgggctacg gtcatggtta
     1321 atacgggaat acggcgacga tacagcgcat gatgtgtcag gcttgaatac ctttatccgt
     1381 taaaagggat atcagttaag ttatcccgtg tagggtataa gccattatca aagccactct
     1441 gtaggaagtg gcttttgtaa tggcaataaa aagccccgcg aatgcgaggc taaatcctgg
     1501 tatttgtaat gactggctct tatctcaacg cagcccctta ccgcgcgcaa aatgctcaat
     1561 atcaagcatc agcaatgaga tgtttaatct ggattcactc cagaagtgag caccaccctg
     1621 tctacagagc cagatgtgaa ggatgatgag taaaattatc gctatcatcg aaggcattgc
     1681 gtcctgatgt attcctgaag cgttctcagt gctgtttggt cgcggataat tccgtcccgg
     1741 ataccgagaa cgtttcgtcc agcaactgga gagagttcga cggtggcatc attgcccatg
     1801 ccggaggcgc tggaggtttc ggctgaggat ggcacagggc attttccttt gacgagcacc
     1861 cgaccaccat tatcaagctt gcgccgaaga gcatcatttt cagctttcgc atcagctaac
     1921 tccttcgtgt atttagcatc gagtgcatca gcatcacgct ggcgctgctg catgtcagta
     1981 atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc tttgtaggta
     2041 atggcgttat cacggtaatg attaacagcc catgacaggc ggacgatgat gcagataacc
     2101 agagcggaga taatcgcggt gactctgctc attgctgccc ccacaaacag acttcacgct
     2161 caatctcacg acgggtcatc agccctttcc attgcttacc gccagcatat gtccagcgac
     2221 gtagctggtc acatgcgcct ttgatatagc cctggtttat tttgcgaaga agcgtcgatg
     2281 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     2341 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     2401 tcaggagagc tttgcattct gcttcggtat acgttttacc aggcatgatg tcttttccgg
     2461 tgtgtccgtg acatacagtc catacaccaa tgatatcttt gtatggtatg tagctgacac
     2521 cttccagacc atcgttacca cctgggccag tgattaacac agatgctata gcaacagccc
     2581 cgccaccaat agcaactgca acagccttgc gtaatgatgg cgacattatt cacctctcgc
     2641 agccttacgc ttatcttctc ttattttgaa atacagatt
//
LOCUS       DAZPLM010000327         2671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPLM010000327 DAZPLM010000000
VERSION     DAZPLM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.330"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHVC7_004243"
     CDS             <1..507
                     /locus_tag="ACHVC7_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2118118.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..982
                     /gene="yqeJ"
                     /locus_tag="ACHVC7_004244"
     CDS             500..982
                     /gene="yqeJ"
                     /locus_tag="ACHVC7_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR2118119.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="ACHVC7_004245"
     CDS             complement(1016..1096)
                     /locus_tag="ACHVC7_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118120.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHVC7_004246"
                     /pseudo
     CDS             complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHVC7_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1986..2476
                     /locus_tag="ACHVC7_004247"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="ACHVC7_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1013 a          430 c          425 g          803 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat
     2521 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
     2581 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
     2641 aatattaatg agtcaatcaa taataattta g
//
LOCUS       DAZPLM010000328         2590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPLM010000328 DAZPLM010000000
VERSION     DAZPLM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.331"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(27..299)
                     /locus_tag="ACHVC7_004248"
     CDS             complement(27..299)
                     /locus_tag="ACHVC7_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR2118121.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(302..580)
                     /locus_tag="ACHVC7_004249"
     CDS             complement(302..580)
                     /locus_tag="ACHVC7_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118122.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(581..1138)
                     /locus_tag="ACHVC7_004250"
     CDS             complement(581..1138)
                     /locus_tag="ACHVC7_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118123.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          722 a          528 c          482 g          858 t
ORIGIN      
        1 caaatattcg aatttaatac ataacattaa atctccagga gtaaattgtt tgggtggcaa
       61 tttcttttat taccatccct gaagataatc tttttagttg cctgaacttc atgatgcaaa
      121 aaccagatga catacttcag ataataatca cgagtgccat aacgaatcat tggattaccg
      181 taagcatcat ctcttgtctt aacctctcca ccatcaatcc attcaagcat tcctgtctca
      241 taattaatca tgaagtaagt tgcaaagaac cgtttaatat catctcgcca ttgcttcata
      301 atcagacctc ctgagacagt ttgtgaattt tgatacttag taaccttgct aaatccagtc
      361 caaaagcatt taaggatttc agactatcaa ggtcatcttg cttaatcctg atatggtgtc
      421 ttgcagcctc taagcgacta ttaagcaaat cctgttcgtt aatatcacgg ttaattgcaa
      481 gcttcatcac tgctgagtgt ggcagacctg taatgttttt gattcggttc agttcatcaa
      541 aggctgtctt tgtaactctg gttaatgtgt gtttattcat ttaaaaatcc tctgtaacta
      601 aataggggtc aaactcgaaa tctggttgct cgttttccat tggctcaata ttcccaacgg
      661 caatatgatg ttcaacaatg ccaattaatt tcgcgcacat cacagcaagc aaagcgcaat
      721 atttatcgct tggtaattcg taactaagtc tggttgcctg tcgctgaaga tgatttgtca
      781 cggtggatgc gttaatttcg tcaagattct ttagcagata aaatagtttt ttgttgtatt
      841 tcgggtggcc ttcacttcta cagaacccaa taaaaacatc atcaagcttg cttggtaact
      901 gctcgtaact gtcaaagtta gccagataat ttttaatagt attctttgtt gttttgcgta
      961 ctggcataaa tctgtctcgt ggcgtaatgc tacgaatgac cgcttcaatg ttcttacgtt
     1021 cggatttgct aaaggttggg acttcgtagc tggtggggtt gtttgtgtaa gcgtaggagt
     1081 tgtgcttgtt gcgttctctt gatgcctgaa gcatcgctct aaattctgaa tactgcattc
     1141 atgttctgac tctcataaga tgttattatt ctgttaacct atatatacaa tattagcatt
     1201 ttcaaagact aatgtcaagt atttttaaca ttttcgtaac atctgtggta taatgggttt
     1261 atggggagag taaaatatga ggcgtggcta tgtatttggt cgcatatttt gcattgcaca
     1321 cagacctctt caatcgccct gtaacgagtt aaacgacctc ttcaggtcaa ctcataaggt
     1381 caggtacttt ttgtgtcaga atgcgattga gagcgtctta caaggaaaac taatggtaaa
     1441 tctccctcct gagctggggt cacctgatgt atggactggt ggaggtattg ggctgattgc
     1501 aggcctgcta cttcgctacg tcctcgtcag agtgcttgtt gtctttaaac tgttcggtcg
     1561 tacatggacg cttgacctta aggataacga ccacacagac ccccgttaaa ctccttcaaa
     1621 acccctgcac gagatgacag atagaagtct atggtacagg ggtgtcttta gcattttgtg
     1681 ctatctatta aaaaatagct tgacagaacc ggaaagctgt atctatcaag cattcacctc
     1741 tctgacagta atttctaaaa gttcttattt ttcaataagt tgtaattagc catatataga
     1801 gaactgacaa gtctcttcac agacaactct gcaaccttaa cagtaacttc taaaaatgct
     1861 tatttttcaa cgagttgtaa tcacccaata tataaggctg tccagtacct tcacagataa
     1921 cttcaagccc tcttaaccgc cccactaatc gggagaagag cgataacccc agagagggaa
     1981 gctcctttct taaccgacct cttaaacgac ctttacagac cttgttcaag gtaatcacaa
     2041 tagtttttct tccctcggtt tcctgtgttc ctcggctggc tattgagatt tgaaagacct
     2101 ctgtaagcgc tctcttagcg agagaaaaat cattcatagt gaccccaagc gaagcgcccc
     2161 tctaaaaaaa gattttttag ctattttgtt taaaagcata tcaaacgata cattttattg
     2221 atttaggtca aagacaaaag cattgacaaa atggtaagat tgttcacatg accttactta
     2281 tttaagagac tttgaaacag cctgtttaac aggtcactac ctctggtctt atcgttccct
     2341 tctttctagg tggttatctg ttaagagttt ttcagttttt attcttaaga taattgttaa
     2401 gatgatactc tcccttcggg ttatatcgta tctcttctcc cgttaaatat cccttcacag
     2461 tcagactaaa taacacctgt gtagttcttg gtagaatctt tcaaggtaac ttagctctga
     2521 gtttatgaag ttgacctatg agatagactc taccgaatct gttagtcaat tgttgcttag
     2581 gtgctgctaa
//
LOCUS       DAZPLM010000329         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPLM010000329 DAZPLM010000000
VERSION     DAZPLM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.332"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHVC7_004251"
     CDS             196..1149
                     /locus_tag="ACHVC7_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2118124.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHVC7_004252"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHVC7_004252"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR2118125.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHVC7_004253"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHVC7_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          636 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZPLM010000330         2563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPLM010000330 DAZPLM010000000
VERSION     DAZPLM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.333"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..805)
                     /locus_tag="ACHVC7_004254"
                     /pseudo
     CDS             complement(1..805)
                     /locus_tag="ACHVC7_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(858..1454)
                     /locus_tag="ACHVC7_004255"
     CDS             complement(858..1454)
                     /locus_tag="ACHVC7_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR2118126.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1481..2083)
                     /gene="yadL"
                     /locus_tag="ACHVC7_004256"
     CDS             complement(1481..2083)
                     /gene="yadL"
                     /locus_tag="ACHVC7_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR2118127.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2098..>2563)
                     /locus_tag="ACHVC7_004257"
     CDS             complement(2098..>2563)
                     /locus_tag="ACHVC7_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118128.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          553 c          513 g          785 t
ORIGIN      
        1 ctaagcatag cggtaaaaca agtgggatta gtcaacttga cattgagcag atcgaaatcg
       61 attttgataa aataactatg ctcatttata ccttggcctg cgccataggc tttaaatgaa
      121 tacagataat ttgatgagct ggataatttg atctgttgag ttactgttgg atcaaagttt
      181 gtatctgtat agaattcaat tgtaagggtc tgataaatac cacctacagc ccagtattta
      241 cgggtgctat tcgcattatt gcaactccaa tataacacgt cttcgcttgg attataccac
      301 gaaaaatatt gttctgcaga gtcaccaatg tatatcccag gtgaactaac gttagttacg
      361 gtaccgtaag ctgaccacat gtttgaaatt aataatgtgt aatacaaacc aggaactgat
      421 gtattaaata atttatggga gccatacatt ttacctgagt caaccattgc attttcaata
      481 cgcattgcgc taccacttgt gctatcactt ctgttgcaat aaagaaaacc accaccagaa
      541 gcggtatcta actgactcca ctgtaatttt acacctgtag gtccaagctg cgtcggtttg
      601 tagaaaacta aattatttgt acttgttgca tatctgaata tgacagtagc atcgccattc
      661 ggccccgttt ctctccattt ttgatctgac ggaccaacgc cgtaactatt cccaacaata
      721 tatcctacgt tatcatcagt gccagcactc gcggtataac aactcaagca cagaattaaa
      781 aaaaatgtct gaagaatttc ttcattatca ctccaccatc tactgctgta acagtcgcag
      841 ttatttgttt tgatctatta ttcgtagtac acatcaacta atatatgact cattacttgg
      901 ccagatatga catccagcgc gggatcgatt ttttgcattc tggcataaaa tttccatgaa
      961 gaattatgca gggcagaagt cattactgga tacacggaac gagaatttcc tgctgaacta
     1021 ataacattga atatattggt cgggtcctga atggaaaaga gaacgacacc gacattttta
     1081 gccccggttg cgacttcatt ggcaaatatt tgccttgaac ctgcagccat gacaccgcta
     1141 agggctctaa aatcaatatg taactgatta agcacatcgc cagttccctg taatgcacag
     1201 tctgatactg tgacagtgaa ctcttttcca cctggttgat agctttcagg agtaacatta
     1261 tctgaaaaat atcccacccc aacgacgcca agatcaatat taccctggtt actgattccg
     1321 cttttgcaag tgctgttttt tacattggca acaaggtcaa tatcctgccc agcgatggct
     1381 ggataggtaa tgggaaactg tagtgacaga aacagaatta ttctcttcat cagcttacgt
     1441 tgagtgggat gcatatatag ttcctggatc tgtcagtctg ttagttatag gttactgtaa
     1501 atgtggcagg tgcagaaaat ttacccgcag tgacattgct tacggtttta tttttttcga
     1561 cgaccagtcg agcactcatc ggataataaa caaccgcgtt ggctgcattg gagatagtga
     1621 cctcttgaga tgctggtgtc acacaactaa attgtgttgc gctccctgtt gccggagttt
     1681 cggtgctcca gacttcaacg gcgacggcac ttgctttatc tgcggctgtg gatccatttg
     1741 cgagccccgc accgtcatta gcagtacctt cgcatgtggc tcgcttggtt aattttattt
     1801 gtgctttttt attggggata cccgcacagt ctttgaattt gagtttgaat gttttaactt
     1861 tggtcttggc attgatttca gaaatatata catcgccaaa gtcaacaaca gaaatgtcct
     1921 gatcagagtc atttactaat gtagcggtac aagtacctat ttctacggta gtaatcacgg
     1981 tcagcccaat gctgtctgtt gccgcataag agggtacgct gaacaatgag gcagccaaaa
     2041 cgacactgct ggataatcca taacgtaaat ttttaaatgt catcttaatt atccttctta
     2101 ttgatagatg aaattaaacg ttgccagtgc acgaaaattc cccggtacgc cttgccctgc
     2161 atctgtttca cgtagcgcaa cggtcatttc aagaccctta tcgggctgca tctcgtctgt
     2221 gctccagcgt atcttttccg cactgttagg tttaaggaaa gtggcatcat cagtagtccg
     2281 ttttttgaaa cccataccga tattacttgt cgtcgaagat gaatcaccag actgcggtat
     2341 aataagctta ggtgagcttg atgatgcatt tccggtcaga gtggtatcaa tccaactgat
     2401 gccactgctg cacccactag caaccagttt aaaatcagcc tgagattctg tcgttttatt
     2461 cgcgatctta tccagaccca tcttagggat tctcaatgtg tagttattat tgccatcatt
     2521 cgtgacgtta ttaccagtaa gtgtgatgtt acaggttgtc gct
//
LOCUS       DAZPLM010000331         2546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPLM010000331 DAZPLM010000000
VERSION     DAZPLM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.334"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHVC7_004258"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHVC7_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHVC7_004259"
     CDS             829..1674
                     /locus_tag="ACHVC7_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR2118129.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHVC7_004260"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHVC7_004260"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR2118130.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHVC7_004261"
     CDS             1861..2061
                     /locus_tag="ACHVC7_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR2118131.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHVC7_004262"
     CDS             2146..2421
                     /locus_tag="ACHVC7_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118132.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          744 a          556 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttaccc
//
LOCUS       DAZPLM010000332         2531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPLM010000332 DAZPLM010000000
VERSION     DAZPLM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.335"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHVC7_004263"
     CDS             <1..656
                     /locus_tag="ACHVC7_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2118133.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHVC7_004264"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHVC7_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR2118134.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHVC7_004265"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHVC7_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR2118135.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHVC7_004266"
     CDS             2258..2494
                     /locus_tag="ACHVC7_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR2118136.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          656 a          559 c          645 g          671 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata a
//
LOCUS       DAZPLM010000333         2497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPLM010000333 DAZPLM010000000
VERSION     DAZPLM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.336"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(11..763)
                     /gene="tcyN"
                     /locus_tag="ACHVC7_004267"
     CDS             complement(11..763)
                     /gene="tcyN"
                     /locus_tag="ACHVC7_004267"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR2118137.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(760..1428)
                     /gene="tcyL"
                     /locus_tag="ACHVC7_004268"
     CDS             complement(760..1428)
                     /gene="tcyL"
                     /locus_tag="ACHVC7_004268"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR2118138.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1443..2429)
                     /gene="dcyD"
                     /locus_tag="ACHVC7_004269"
     CDS             complement(1443..2429)
                     /gene="dcyD"
                     /locus_tag="ACHVC7_004269"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR2118139.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          598 a          710 c          621 g          568 t
ORIGIN      
        1 ttttttctta ttattgcagc agaaacttct cgaggaactg gcgggtgcga ggctgctggg
       61 gatcagcaaa taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaaa
      121 tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc
      181 gcttttcctg cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca
      241 gcgctgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagc gcacgcgcaa
      301 tcgctacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg
      361 ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc
      421 ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat
      481 taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcggatcagc gatttttgct
      541 gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg
      601 gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga
      661 taattgccac cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac
      721 cgtgaaattt tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg
      781 gcgattaagt tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca
      841 gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa
      901 ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcctttac
      961 caggctgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat
     1021 cgcgcgacgc atagtctgcc atggcgtcat accaatactg gcagctgctt cccactgacc
     1081 tttatcaata gaagaaatgg cggcgcgcag tgtttcggcg gcataggcgg cagtgttcag
     1141 cgacagacca atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc
     1201 gtaatagatc ataaacagtt gtgcgatgag cggtgtacca cgaaaaatag agatataaaa
     1261 acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc
     1321 gagcagtaag ccaaaaaaca tgccgccgat actgagttgc agcgtatacc cagccccttt
     1381 cagcaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg
     1441 agctaaacgt ggggatgata ggcgaacagc gcaggcgcac cgccggtatg aataaacaga
     1501 atcggccctt catctttgaa gcgtttctga ctgatacagc caatcagccc cgccatcgct
     1561 tttccggtat acacaggatc aagcagaatg ccttccagcc gcgccagcaa tttcactgct
     1621 tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtata
     1681 atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact
     1741 ttcggcaact gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc
     1801 atcaggtgtt ccagcccaac agccagtccg gcgtgagttc cggcactgcc cgatgcgact
     1861 accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc
     1921 acataaccta gcgcgccaag agcattagaa ccgccaaccg gaatgacata cgggcgaaag
     1981 ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgct
     2041 tcgcacattt caatctgggt attgaacaga tccagcaaca agcgattgcc gttggttaaa
     2101 tagttttctg cggttgtgcc aataggattt tccagcagcg ccacacagtg cagaccgagt
     2161 ttcgccgcaa ctgcggcagt ctgccgcaca tggttagact ggatcacccc ggcagtaatc
     2221 agcgtatcgg caccttcgcg cagagcatct gccgcgagaa attctagctt acgtaattta
     2281 ttgccgccca ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga
     2341 taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt
     2401 ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtatgttg ttgtgatttt
     2461 cttattatgc acgctgaaaa cgcgtaaata aaaaagg
//
LOCUS       DAZPLM010000334         2496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPLM010000334 DAZPLM010000000
VERSION     DAZPLM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.337"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..289
                     /locus_tag="ACHVC7_004270"
     CDS             <1..289
                     /locus_tag="ACHVC7_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118140.1"
                     /translation="LVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNM
                     TLRLRLQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(679..813)
                     /locus_tag="ACHVC7_004271"
                     /pseudo
     CDS             complement(679..813)
                     /locus_tag="ACHVC7_004271"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(826..1647)
                     /locus_tag="ACHVC7_004272"
     CDS             complement(826..1647)
                     /locus_tag="ACHVC7_004272"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR2118141.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1636..2442
                     /locus_tag="ACHVC7_004273"
                     /pseudo
     CDS             1636..2442
                     /locus_tag="ACHVC7_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1916..2032
                     /locus_tag="ACHVC7_004273"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          657 a          564 c          584 g          691 t
ORIGIN      
        1 gctggtcact gacaccataa cagaggccac cctgtacgaa aacgggctgt atacgcgcca
       61 catcagactc agtcgcccag tatctgacag caaatacccc ttgccacccg gatctcagct
      121 cattaaaaat atgaccttac gactgcgcct gcaggatcag caggaagaaa catccgtgac
      181 attatttcag ggaaaaatga gtaccgacgg aaataactat tacatcatca aaggtaaagt
      241 aagctcagta cttctgttat taaaaatgct gcagattaat cacgcctgat ctctgtcccg
      301 gtattttagc ccgaggtata ttctgaaata tcattcatca tgaccggcgt ttatacaaac
      361 gccggtcatt cgctaacagc ttcaccgttc cttcccccat ccaggttccg catgagcgtg
      421 ttgtaatgtt cataagcgta ttatcaggat tgtctggaaa attcccgata ctttggacta
      481 ttactcctga acaattcgaa attcagcaga ttaaaggtaa gcgttaacgg aggtaagcgt
      541 taacggaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtaa accgactgcc
      601 gtatgtagcc attaagccag tattggtaac gtaggtgccc accttttcaa atgatctgcg
      661 ccccaaatct tggacatcct atgaaaaatg tttaagtcga tattgaaccg gactcatgcc
      721 atcgagtttc tctttaatgc gatggtgatt gtagtattca atgtaatcaa ttatatcttg
      781 ctttagatga tgtaacttat tgtatttatt taggtagtaa cattcttatt ttaaagtact
      841 aaaaaagctt tccattgctg cattatctaa gcagttacca cgtcgggaca tgctttgtgt
      901 tatgccatta tcactcaacc aacgttgcca acgaggcatc tgatattgcc acccctgatc
      961 actatgcaac aagggcttat cgtctttatt gagttgttct accgcatcta atagcatgct
     1021 ctttaccatt gatgctaatg ggcgctcagt aaggttatat gagactattt ctccattata
     1081 caggtccatg attggtgata aatagagctt cctgccatcg accttaaact ctgtgacatc
     1141 tgttacccac ttttgatttg ggcggtcagc ataaaattgt ctcttcagta aattattaca
     1201 agttcgcccc tgttctccac gataggcacg gtacttcttg cgcctgataa gtgagctgat
     1261 acccagttcc ttcattaact ttctgatggt tttaccatta agtaaaatgc cctcgttacg
     1321 gcaggcaagt ctgatgcgtc gataaccata acgcccttta tgctgatggt atattctcaa
     1381 tattacttgt ttaacaagtt gatatggatc tggcgcatta aggcgtttca cgtgatagta
     1441 aaatgtacta cgtggcaaat ccgcgataac cagtaattgt gataatggat gacattgcct
     1501 taactcatta ataatgatta ctttttcttt tgttccaatc gtaatttttc ctgagctaag
     1561 gctttcagct tttttagata atgaaccgcc ccgggaatcc tggagactaa actccctgag
     1621 aaagaggtaa acaggatgac taaaaatact cgttttcccc cgaagtccgt caacgggcag
     1681 tccgtatggt tctggaaagt cagggcgaat atgactcaca atgggcggta atttgttcca
     1741 ttactccaaa gattggctgt acaccggaga ctctgcgtgt ctgggttcgc cagcatgagc
     1801 gggataccag gggcggtgat ggagggctca ccaccgctga acgtcagcgt ctgaaagagc
     1861 tggagcgtga gaatcgtgaa ctgcgctgca gtaacgatat ccttcgccag gcttccgctt
     1921 attttgcgaa ggcggagttc gaccgcctct ggaaaaaata atgccactgc tggataagct
     1981 gcgtgggcag tacggggtcg gaccggtatg cagtgaactg catattgccc cgtcaacgta
     2041 ttaccactgt tagcaacagg acatcatcct gataaacgca gtgcccgtgc gcagcacgat
     2101 gactggctga agagagagat acagcgcgta tacgatgaaa atcatcaggt gtacggtgtg
     2161 cgtaaagtct ggcgccagtt gttacgggaa ggaatcaggg tggccagatg tacagtggcg
     2221 cgcctcatgg cggttatggg acttgtcggt gttctccggg gtaaaaaggt ccgcactacc
     2281 gtcagccgga aaaccgttgc cgcaggtgac cgcgtaaacc gtcagttcgt ggcagaacgt
     2341 cctgaccagc tgtgggtggc tgattttact tacgtcagca catggcaggg cttcgtctat
     2401 gtggcgttca tcattgatgt gtttgccgga tacatcgtgt ggtgacgagt ctcatcgtct
     2461 atggaaacga cattcgtgct ggatgcactg gagcag
//
LOCUS       DAZPLM010000335         2484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPLM010000335 DAZPLM010000000
VERSION     DAZPLM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.338"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            275..418
                     /locus_tag="ACHVC7_004274"
     CDS             275..418
                     /locus_tag="ACHVC7_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR2118142.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            431..640
                     /gene="cspE"
                     /locus_tag="ACHVC7_004275"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             431..640
                     /gene="cspE"
                     /locus_tag="ACHVC7_004275"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR2118143.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            806..1615
                     /gene="rlmA"
                     /locus_tag="ACHVC7_004276"
     CDS             806..1615
                     /gene="rlmA"
                     /locus_tag="ACHVC7_004276"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR2118144.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1612..2178)
                     /gene="mntP"
                     /locus_tag="ACHVC7_004277"
     CDS             complement(1612..2178)
                     /gene="mntP"
                     /locus_tag="ACHVC7_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR2118145.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          656 a          589 c          602 g          637 t
ORIGIN      
        1 attgcacgca ctatgtaata acttctccca ctggcctgga acaactgaac ttattgaact
       61 atgttagaaa atacgccagt ttaagtatct gcctgaactg gcaaggttaa gcacaatgat
      121 atatcggcgc gtattccgtt gcataagtgt gcaaaaaaag tggaagacgt atcgagattt
      181 gtgcgtctga tcgagacatg tttaaaaatg gcttgccata attaacgttg tatgtgataa
      241 cagatttcgg gttaaacgag gtacagttct gtttatgtgt ggcattttca gtaaagaagt
      301 cctgagtaaa cacgttgacg ttgaataccg cttctctgcc gagccttata ttggtgcctc
      361 atgcagtaat gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa caatctaagg
      421 aatttttcaa atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt
      481 tggcttcatt actccggctg atggcagcaa agacgtgttc gtacacttct ccgctatcca
      541 gggtaatggc ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg
      601 ccagaaaggt ccggcagctg ttaacgtaac agctatctga tcgaatccac tgatctgaag
      661 tgtgaatacg cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt ttactatgct
      721 ttatcttcgc tcctggcgtt cggatatttg cccgccgcgt gattcgcgtt acacttgcgg
      781 cctttagtat cctgccggag ttgtcatgtc tttttcctgt ccactttgcc atcagcctct
      841 ttcgcgtgaa aaaaacagct atatctgtcc ccagcgacat cagtttgata tggcgaaaga
      901 agggtatgtc aatctgctgt ccgttcagca taaacggtct cgtgatccgg gcgacagcgc
      961 ggaaatgatg caagcacgcc gcgcattctt agatgccgga cattatcagc cgctgcgtga
     1021 tgcaattgtc gcccaactga gggaacggct tgatgataag gccacggcgg tgctggatat
     1081 tggctgtggt gaagggtatt acacacacgc atttgccgat gcgttgcccg aaatcaccac
     1141 gtttggtctg gatgtttcga aggtagcgat aaaagcggcg gcgaaacgct atccgcaggt
     1201 cactttttgt gtcgcttcca gccaccgttt gccgttttcc gataccagta tggacgccat
     1261 aatacgtatt tacgcgccgt gtaaagcaga agaattagca cgagtagtga agcccggcgg
     1321 ctgggtcatt actgccacgc cgggaccgcg acatttgatg gagctgaagg ggctgattta
     1381 caatgaagta catcttcatg cacctcatgc agaacaactg gaaggtttta cattacagca
     1441 gagtgcggag ttgtgttatc cgatgcgtct tcgcggtgat gaagccgtcg cattattgca
     1501 gatgacgccg tttacctggc gtgcgaagcc agaagtctgg caaacactgg cagcaaaaga
     1561 agtgttcgac tgccagacgg actttaatat tcacctctgg cagcgttctt attaaccgtg
     1621 gaagtgcgtc cagaggatct ggacgccgat gccgatcagt accagcccgc cgagaatttc
     1681 cgcttttttc ccaataattg agccgataaa gcgaccaacc atcatcccta atgttgacat
     1741 aatcaaggtt gcacaaccaa tggccaatgc ggtcgcgata atgttgacct gcaggaaagc
     1801 aagaccaaca cccacagcca tggcatccag gctggtggca atcgcggtgg ttaccagtag
     1861 ccagaaaccg tgtcgacggc gcggctcttc atcttcatca tctgcgccac gaaaaccctc
     1921 aataatcatt cgcccgccga ggaatatcag cagcacaaac gcaatccagt ggttccattc
     1981 aaggacaaac cggctggcta acatgcccat tccccagccg atcagcggcg tcagggtttc
     2041 gacggcacca aaaataaggc cggttcgcaa tgcttcagaa aatttcggtt tatggagggt
     2101 ggcaccttta ccgattgatg cagcaaatgc atccatcgac ataccaaacg caagaagaac
     2161 agtagcagtg atattcatga caatgtcctg accggggtat ccataaaaca catcatcgcc
     2221 cccagcaaac atgcatacat gaacaacaag ccgtgatggt tttcgcgttc atgcaggcat
     2281 agcgattgat gtgcatatgg tctcgcctga ctgaaagtat tcagtccgta cgtgccacgt
     2341 tttgcaacga gtatgttgac acgtacattt ccggaatctg gaaatcggct actccccaaa
     2401 tgagggcgca accttaacat attttgagaa tataaaacaa ctgttggaat atattatttc
     2461 attgctttat tgataacgat tttc
//
LOCUS       DAZPLM010000336         2431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPLM010000336 DAZPLM010000000
VERSION     DAZPLM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.339"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(168..1541)
                     /gene="cysG"
                     /locus_tag="ACHVC7_004278"
     CDS             complement(168..1541)
                     /gene="cysG"
                     /locus_tag="ACHVC7_004278"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR2118146.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1560..2366)
                     /gene="nirC"
                     /locus_tag="ACHVC7_004279"
     CDS             complement(1560..2366)
                     /gene="nirC"
                     /locus_tag="ACHVC7_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR2118147.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
BASE COUNT          624 a          665 c          602 g          540 t
ORIGIN      
        1 caggaatatt taattggcgt cagtgagcga atgacttaat tttgtaagtc gctgtaacgg
       61 tgaatataac ccattagcag ataatattta ttaaggggtt atgttttatt tgctgaatat
      121 ataaaaagag tcattaagaa ggccagaatt ctctggcccg ggttaaatta atggttggag
      181 aaccagttca gtttatcgcg caggccaaca acccgaccaa taataattag cgatggactg
      241 ttcatttgct gagcaagttc accgagttgc gtgagcgtac cgtcaatcac gcgctgcgtg
      301 actgccgttc cgttttcgac aattgccacc ggcatttcgc caggcatacc gtgttcaatc
      361 agcttttgct gaatagtcgc ggcctgattc aatcccatat agaacaccag cgtctgtttt
      421 tctgccgcca ggttttccca gtccagctcg ccaccggttt ttaagtgtcc ggtaattaag
      481 cgtacgctct gggcataatc gcgatgcgtg agtggaatac ccgaataggc agagcaacca
      541 gaagctgcgg taatacccgg aaccaccgag aacggaatac ccgcgttgca cagtgtttcc
      601 agctcttcgc caccacggcc aaaaataaac ggatcgccac ctttcagccg caccacgcgt
      661 ttgccttttt gcgcttcccg cagcaggatc tggttaatct cttcctgcgg tacacagtgg
      721 tatcccgcgc gtttgccgac gaaaacgcga tcagcatcgc ggcgtaccag attcataata
      781 tcgtcagaaa ccagacggtc gtagaccacc acatctgcct gctgaatttg ttgcagtcct
      841 ttcagcgtca gcagcccggc atcgcccgga cctgcaccaa ccagcaccac ttcaccgcga
      901 tggtcgagcg gttcgttgat taactgttcg gtcgtttcag taatggcttt ctgatcgttg
      961 tttgccagcg actgcgccag gcggtcgtta acgaacaatt tctcccagaa acggcgacgc
     1021 tcactcatcg tggcgaactg ttgtttcact cgcccgcgta attgcccggc atatttcgcc
     1081 acctggccca gatgcaacgg cagcagtgat tcgagttttt cgcgcaacag gcgcgccaga
     1141 accggagagg tgccgccaga ggagaccgct accattagcg gtgagcggtc aataatcgac
     1201 ggcataataa agctggcggc tttcggcgca tcgactacgt tacagaagat gcgacgagct
     1261 tcagcggctt cgctgacgcg ctggttaagc gtgtcatcat ccgtcgctgc aatcgccagc
     1321 cagcaggtgt cgagaaggct ttcatcaaat ggcccttcga cgagggttaa catgcctgca
     1381 tctgcccatg cggtgaactg tggaataaac gctaatgcat tcaccgttaa gcgagcgcct
     1441 gcgtctaaca gcaaccttgc tttgcgttcc gcgacatcac caccgccgac aatcagacag
     1501 tcgcgatcgc gtaattggca aaatataggc aaatgatcca cgtaaaaacc ccttagtaat
     1561 taaccggcag ccgtttcagt ttgattaaat ttgtccgcaa ccggacgatt cgctttcggc
     1621 gtagcatacc aataacccaa tcccatgaat acggcacccg ataaagtatt acccagcgtc
     1681 acccacagca ggttatgacc aatacccgcc agcgtgtagg cttcgctgtg gttgccgaac
     1741 caggagaggg cgaacagcgt catgttagcg atggagtgct cgtagccgga cgcgataaat
     1801 gccagcagac accaccagat agcgataaat ttcgccgccc cttcagtgcg cagcgccatc
     1861 cagatagcca ggcaaaccag ccagttacac aatgcacctt tgaagaacag taccattgcc
     1921 ggtgcagtgg ttttagccag cgcgacggag tgaacgatgc tggtatctac cggcagcagg
     1981 ctaccgccgc cccagctata gagcatggca acgaagacgg aaccgaccag gttacccagc
     2041 caggtttgcg gcaggattgc ccacatttgc ccgtggctga tgctgcccgc ttttacccca
     2101 aaggtgagga acatggcgtg tccggtgaac agttcagaac cggcgataat caccagcgtt
     2161 aaggcgatac caaaggtcgc gcccatcacc agcggacgta cggacgggtc gagcaaatta
     2221 ccgagcgtga aaatcaggat gatcccaaga cccacatatg cgcccgccat ggcggagctg
     2281 acccaaaagc cgagcgggtt atttgccgac aggcgtgcaa tgcgcgcagc attagccgca
     2341 cacttattaa tagtgtctgt aaacatttga ttatccttta aaaaataaaa acaaaaaata
     2401 aaacaaaaag tttaaaaaat gttctgcaat a
//
LOCUS       DAZPLM010000337         2404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPLM010000337 DAZPLM010000000
VERSION     DAZPLM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.340"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..448)
                     /locus_tag="ACHVC7_004280"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="ACHVC7_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(812..1474)
                     /locus_tag="ACHVC7_004281"
     CDS             complement(812..1474)
                     /locus_tag="ACHVC7_004281"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR2118148.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(1844..2395)
                     /locus_tag="ACHVC7_004282"
                     /pseudo
     CDS             complement(1844..2395)
                     /locus_tag="ACHVC7_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          687 a          514 c          437 g          766 t
ORIGIN      
        1 ttattatcta atgttttatc taataactta tctctctaac acgggtgaca ttttgtcact
       61 acccccatga cattttgtca ctggtagtgg tgtcattttg tcttactgag gctgcaactt
      121 cctgtgatgt aggtcttaca agtaattccg gcttatcatc cagtgtgaat acacgataag
      181 tgctttgacg cccctgattc ttttgcacct gaattaaacc gtgtttctca agtgtctcaa
      241 tggctctgat tgctgttctc tttgatacac cagccatata agcgagatgg tcaatacttg
      301 gtgaatatga tttgtatttc tcttcataga agtcacagct atggaccatg accatataaa
      361 tcaatcttgc tgctgcgtta atttcttctg aaagttgaga gattcgcaca tcaaatttat
      421 aaaactgatt tgggttatat ttagacattc ataaattacc tcatacgtga aaaggccaac
      481 cggataaaac cgattagcct gtgtgattaa aatatctggg ggattgatgg actgataaag
      541 aagtccatgc ctgaaacagt acgatgatta aaacgctgag gtataccctg aatgagaggt
      601 taatatacgc caaccatctg acacctcccc aagtgttacc acttcaagac atgaactttt
      661 ttatcagccc atatagaaac tataacagat gtgattataa attgcaacaa ttatttcaca
      721 gaatgattac tgattggtca tttattgttc acagggatac ttcttaacat ctgaatcacc
      781 tgtcttgtaa atgaccatat ttatgagaat tttagctcaa cctttgagca atcctgtatg
      841 acgtgctcag aggcattctg agagcatctg caatgtactt aatgggtttt ccttgcctgt
      901 atagttttat gaagctgtcg agcgtctcag gtgggtaaat ggtgggtctt cctcttagtc
      961 caccacggtt aatgatggtt aacggtagca atgtatcagc acagcaaacc atctgtgtaa
     1021 tttcatgctt acgtggttct tttttaaaaa caacgactgc acttggaaac ggtgacctaa
     1081 tagtaagcct gttatgccct gctggtggaa ggtatttaac tgcacgatga agaatacgga
     1141 tttcatgaca gtggttattg ctgtaaatgt gcctaagcca gtagctggtg ttggctctat
     1201 gcggtatcag gaaagctact aaatccgctt cagacgcttt taagaggaac gacggaatat
     1261 cgctgtacgg cgggttacag aagacctttt cactaaccca tgattgacgg cttatatcat
     1321 cggtgaatcg tggtaacagc gtgtcatgct cagttgcagc accatctaga ctgaaaataa
     1381 attcacggtg taagttctta aataacaaaa atggagttct ccagtaatca gaacccccat
     1441 gatgcttccc gattaaaata tgctcaattg acataagaaa taaaaatttt tattgcaatc
     1501 actcttgaca aggtttactc tacttttaag agatacttaa caggttttct aaacggatat
     1561 cctttctggg atatctatct tttatcttaa aagtttttac agatacttta aagatatctg
     1621 tatatattct gtacagtaac ttttcagagt ctgaaaattt ttataaaaaa tgactagccc
     1681 tctttgaatc tcatcttccc cgaatcttcg aaaatatctg tctagttgat tcggtagaat
     1741 ctacctcatg atgtatacgt gtttagtacc cgtcacacca gctatgtgac ccccttggca
     1801 aaaaagccaa atatcagcta catccatgta actattcagt aacttattta gcaagtctgt
     1861 aataagttgc tctagagatt cctagctctt cctgaatctg caacctcgtt ttaccttgtg
     1921 aaataagcat ctgtacagcc tctgaagaag cttttacagg tcttcccata tgtacccctt
     1981 aagctttagc acgttcaata ccagcctttc tacgctctgc tatcaagctt ctttcaagct
     2041 gtgccaacgt tgacataatg cctaacattg cttcacccat agcgcttgat aagtcaaatc
     2101 cttctctaag gctgataact gtcacacctt tagcctgtag cgctttaacc gttgataaca
     2161 catcaattgt gtcacgtcca agacggtcta cagctaccac aactacggtg tcaccttctc
     2221 gtgcataagc taacaaactg ctgaaaccgt ttcttaaaga agctttgaca gaaccactga
     2281 cagcttcatc agcgaaccat ttagcaatgt tgtaccctgc ctcttcaatt tgcatttgct
     2341 ggttagtgat ggattggtca tctgtactca ccctgcaata tccaagtatc gccataatgt
     2401 tccc
//
LOCUS       DAZPLM010000338         2382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPLM010000338 DAZPLM010000000
VERSION     DAZPLM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.341"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1075
                     /locus_tag="ACHVC7_004283"
     CDS             <1..1075
                     /locus_tag="ACHVC7_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2118149.1"
                     /translation="VTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGD
                     KTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSL
                     DYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLNF
                     AAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERA
                     AANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQ
                     YQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDE
                     SDFTRAVDIRTDNIVATGITYQF"
     gene            1239..1721
                     /locus_tag="ACHVC7_004284"
                     /pseudo
     CDS             1239..1721
                     /locus_tag="ACHVC7_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1836..>2382
                     /locus_tag="ACHVC7_004285"
     CDS             1836..>2382
                     /locus_tag="ACHVC7_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2118150.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          773 a          487 c          441 g          681 t
ORIGIN      
        1 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
       61 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
      121 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
      181 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
      241 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
      301 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
      361 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
      421 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
      481 tgccgcgcaa tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga
      541 ctacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
      601 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgctgc
      661 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
      721 tgccaacaac atctactttg cagctaatta cgctgaaaca ttaaacatga ccacctatgg
      781 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
      841 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
      901 cggcgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
      961 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
     1021 cgtagatatt cggaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
     1081 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
     1141 aagtaaaaat gagcaatttt tgatatttct ggtcattcac ctcaattaat attattccat
     1201 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
     1261 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
     1321 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
     1381 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
     1441 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
     1501 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
     1561 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaatcaaaac
     1621 tggaaaacat ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt
     1681 cattggttaa tctggaagcg caaaataaca aactgacaaa aattaatatt tctgatagtt
     1741 ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat gtctcccggc
     1801 aagaatctct gattgagtta agtgcccatc acaatatgat cactgacctt atattacaca
     1861 atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat ttaaacgcca
     1921 aaaacactac aaaactggaa tatttaaact taagcaataa caatttactg ccaaccaatg
     1981 acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac ggcatcaaca
     2041 atgatccact tgcacaaatg cagtactgga ctgcagtaag aaatataatt gatgacacta
     2101 atgaagtgac cattgattta tcaggactta atttaaccac tcaaccacca gggctgcaaa
     2161 acttcacatc tatcaatctt gataataacc aactcacaca ttttgatgca accaactacg
     2221 atagactcgt aaagctaagt ctgaatagta atgctcttga gtcaataaat tttcctcaag
     2281 gcagaaatgt aagtattaca catatatcta tgaataataa tgctctcaga aatattgata
     2341 tagataggct ttcatcagtt acttatttta gtgcggcaca ta
//
LOCUS       DAZPLM010000339         2373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPLM010000339 DAZPLM010000000
VERSION     DAZPLM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.342"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..667
                     /locus_tag="ACHVC7_004286"
     CDS             <1..667
                     /locus_tag="ACHVC7_004286"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2118151.1"
                     /translation="AVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGRAVT
                     KLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLACRRRGLIVL
                     TDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLE
                     VACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTAR
                     GYH"
     gene            complement(716..2215)
                     /gene="pitB"
                     /locus_tag="ACHVC7_004287"
     CDS             complement(716..2215)
                     /gene="pitB"
                     /locus_tag="ACHVC7_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR2118152.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2152..>2373
                     /locus_tag="ACHVC7_004288"
     CDS             2152..>2373
                     /locus_tag="ACHVC7_004288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118153.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIK"
BASE COUNT          639 a          601 c          575 g          558 t
ORIGIN      
        1 tgccgttatt ggtagcgatg gttcaggcaa gtcaacggtg tgtgaacatc ttattaccgt
       61 tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaggccg gaaatgtcgg
      121 tcgtgcagtg acaaaattac cgttgatggg aaaatcctta cataaaacaa ttgaacgaaa
      181 tcaggtgaaa acagcaaaaa aattgcctag accagttccg gcgctggtaa ttacagcgtt
      241 tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc gcgggttaat
      301 tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg gtacggtgtt
      361 cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg aacgtaaagc
      421 gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg ttgaccttga
      481 agttgcctgt gcacgtaaac ccgaccataa acgggaatcg ctggcgagga agattgccat
      541 aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca atcagccact
      601 ggaacaggtg ttggttgatg cagaaaaagc gattacggat tttatgaccg cgcgtggtta
      661 tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag gaaggttaaa
      721 tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc ggtaaagtaa
      781 acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg ccgtccacca
      841 ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt ggagacaggc atcccaatat
      901 aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct tgcgcatacg
      961 tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc cagccaatca
     1021 tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacacagga gcgtactcaa
     1081 tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct ttactgacgc
     1141 ctggcagttt cgccagcttc gcggaggtat cagagatgca cagcataatg cggcgcagct
     1201 ggctgcgctg actcacgctt aacggctcgt aactttccat attgcctggc agcatcgttt
     1261 taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactct gttacttgcg
     1321 tgccatcagt cgatgctgca ggcaatggag gttccatcgc aatcaacttc tgcggcagtt
     1381 caggatgctg ttgcaggtag tgttcgaagt tggtgacggc atcgcgggta cgggtaattt
     1441 catagccgga cgcgttcata ttgacgacga agccagcagg ggcaatcccc accagtacca
     1501 gcatcaccag gccgatccct ttttgtccgt cgttcgcgcc gtgcgaaaac gccacgcccg
     1561 cagcggaaac aatcagcgca atacgcgtcc agaatggcgg tttacgtttg ccttttttct
     1621 ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtcccg ctccagtagc
     1681 gtcgcagcag gaatatcagg cctcccgcaa tgaccaggcc gacgataggg gaaacaatca
     1741 gcgaggagaa aattttggtc acttcacgca ggtttaacgc atccatcacc gatgatccgg
     1801 ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg tgcgaactgg
     1861 aggccggtaa accgaagaac cacgttccca ggttccagat aatcgccgcc agcagcatgg
     1921 aaaagaccat cgccaggccg tgggttgacc ccatattcag caacaaatcg gttggcaaca
     1981 tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa aagttaaaaa
     2041 atgccgccat caccacagca agttgtggtt gcatggcacg agtataaata acggttgcca
     2101 ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca aatgccagag
     2161 caagcaataa caaaagccct gtgtatatat caaggccaac aaataaattt agcatatatt
     2221 agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa cggcatgggc
     2281 aaagtgaaat atcatttttt aacgataaaa agagtgttat ttcttgtgta tcagacaata
     2341 agcattcatt caaaaccttt agaaataatc aaa
//
LOCUS       DAZPLM010000340         2352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPLM010000340 DAZPLM010000000
VERSION     DAZPLM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.343"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHVC7_004289"
     CDS             <1..172
                     /locus_tag="ACHVC7_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR2118154.1"
                     /translation="FVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFT
                     NFNTIERIENYL"
     gene            169..648
                     /locus_tag="ACHVC7_004290"
     CDS             169..648
                     /locus_tag="ACHVC7_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR2118155.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(702..1652)
                     /gene="corA"
                     /locus_tag="ACHVC7_004291"
     CDS             complement(702..1652)
                     /gene="corA"
                     /locus_tag="ACHVC7_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR2118156.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1804..1860)
                     /gene="ysgD"
                     /locus_tag="ACHVC7_004292"
     CDS             complement(1804..1860)
                     /gene="ysgD"
                     /locus_tag="ACHVC7_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR2118157.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2022..>2352
                     /locus_tag="ACHVC7_004293"
     CDS             2022..>2352
                     /locus_tag="ACHVC7_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118158.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          668 a          548 c          529 g          607 t
ORIGIN      
        1 ttttgtagca caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag
       61 caaatataga ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa
      121 accttttttc accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa
      181 ctccagaaaa ttatattatg tttatttctt ctatgctgca tctataattt atggacattg
      241 cgtcctgtac agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt
      301 gatcatctgc catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa
      361 attaaaaatc aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc
      421 ggtaatggct tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt
      481 ttcccgacaa ttaagagcaa tgataattgc atcgtgaaaa attacttaat ggttattaat
      541 gaataccctt atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca
      601 caagaaaata aaatagagcg agttttcaat ccgcatgact ttaaataaga caatttttag
      661 aaataagccg gataaaaaaa tagctttatc cggcactcat attacagcca gttcttgcgc
      721 ttaaagtaca gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag
      781 ccgaaactcc acttcagttc tggcataaac tcaaagttca tcccatagct ggaagcaacg
      841 agcgtcggcg gcaggaatac cacggatacc accgagaaga ttttgatgat gcggttctgc
      901 tcgatgttga taaaacccat cgccgcctgc atcaggaagt tcaccttctg gaacagggat
      961 tcgttatgcg gcagcaggga ttcgatatct cgcaggattt cacgcgcctg ctccagttgc
     1021 ccacccggta aacgcgcttt acgcaccagg aagttgagcg cgcgctgggt atccatcaga
     1081 cacaggcgaa ctttccagcc gatatcttcc agttccgcca gagtggagag cgcctcgtcg
     1141 tactcatcgc cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag
     1201 atattttcaa tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcagc
     1261 tcgtaggcgt taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa
     1321 gcgggcagtt cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca
     1381 gtggagttac cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg
     1441 ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt
     1501 gccaggctct ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca
     1561 agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt
     1621 cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc
     1681 ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc
     1741 agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata
     1801 gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa
     1861 ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca
     1921 acacgcaaca gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag
     1981 gtcgctaaca ggataaaaaa atccaacatc caggacctat aatggcgcat cggctactca
     2041 ttggtaaagg aatgatcacc ttgaatctca aacggatttt tctcgccctc accttacttc
     2101 ccttgtttgc tgttgccgct gatgattgcg cactctcaga tccgatgctg accgtacagg
     2161 cgtataccgt taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg
     2221 aagcgtgggg aactttacat gctctgctgg tggatatgaa tagtcagggt caggtgcaga
     2281 tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa
     2341 acggtcagca ga
//
LOCUS       DAZPLM010000341         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPLM010000341 DAZPLM010000000
VERSION     DAZPLM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.344"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHVC7_004294"
     CDS             complement(25..315)
                     /locus_tag="ACHVC7_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR2118159.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHVC7_004295"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHVC7_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR2118160.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHVC7_004296"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHVC7_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR2118161.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHVC7_004297"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHVC7_004297"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2331)
                     /locus_tag="ACHVC7_004298"
     CDS             complement(2171..>2331)
                     /locus_tag="ACHVC7_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR2118162.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLG
                     TIRAFDVT"
BASE COUNT          643 a          621 c          516 g          551 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc t
//
LOCUS       DAZPLM010000342         2261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPLM010000342 DAZPLM010000000
VERSION     DAZPLM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.345"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(123..1127)
                     /gene="argF"
                     /locus_tag="ACHVC7_004299"
     CDS             complement(123..1127)
                     /gene="argF"
                     /locus_tag="ACHVC7_004299"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR2118163.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1289..1705
                     /gene="rraB"
                     /locus_tag="ACHVC7_004300"
     CDS             1289..1705
                     /gene="rraB"
                     /locus_tag="ACHVC7_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR2118164.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1751..2254)
                     /locus_tag="ACHVC7_004301"
     CDS             complement(1751..2254)
                     /locus_tag="ACHVC7_004301"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2118165.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          574 a          559 c          551 g          577 t
ORIGIN      
        1 cctccggaag atttacgtat agcaataaaa aaattggggt ggaataattg aactatcact
       61 gcataaaata atgtgacgcc gggatgtttt tgtatttccc ggcatcttta tagcgatatc
      121 aattatttac tgagcgtcgc gaccatcacc gctttgatgg tatgcatgcg gttttccgcc
      181 tgatcaaata caatgctggc ggcagattcg aagacctcat cagtcacttc cattccgcca
      241 tgtaggccaa actcttccgc catttttttg ccaagcgtcg tttggtcgtc atgaaacgcg
      301 ggcaggcagt ggaggaattt gacctccggg ttaccggtca actgcatcat cttgctgttc
      361 acttgataat cacgcagcaa tgcaatgcgt tcagcccatt tctcttttgc ttcacccatc
      421 gacacccata catcggtata gataaagtca gcaccttcaa ctcctttagc tacatcttcc
      481 gtcagcgtaa tattcccgcc attttgttgt gccagggcgc ggcattccgt aaccagcgca
      541 gcttccggcc agcatgcctg tggcgcgacc agacgcaaat ccagaccggt aagcgctgca
      601 gcttcgagca tcgaattgcc catgttgtta cgtgcgtcac ctgcatagac tagcgtcatt
      661 tcgttgaacg ttttgccggg caaatgctcc tgcatggtga ggagatccgc cagcagctgc
      721 gtggggtgaa actcattggt caggccgttc cataccggca cgccagcgta ttccgccagt
      781 gtttcgacaa tctcctgacc atagccgcga tactgaatac cgtcatacat gcgaccaagc
      841 acgcgggcag tgtctttaat cgactcttta tgaccaattt ggctgccgct tgggccgaga
      901 taagtaacac gagccccctg gtcatatgcg gcaacttcga aagagcatcg ggtacgggtc
      961 gagtcttttt cgaagatgag cgcgatgttt ttaccagtga gtttggcttc ttctttaccg
     1021 cttttcttat cggctttcag cttcgcggct aactgcagca ggctgttgag ttcagctggc
     1081 gtgaaatcga gtaatttcag gaaatgctta tgataaaacc cggacatagg tccctcctgt
     1141 ggctaacgcc ttaatgaatt aaaattcaat ttatatggat gattattcat ttgcaagtct
     1201 aaagcataaa tctttatcac aaaggtggag gcaatgtcag tggtgtgtga caataagagt
     1261 atcggcagga cattaagagg aatgagccat ggcaaacccg gaacaactgg aagaacagcg
     1321 tgaagaaaca cgtttgatta ttgaagaatt actggaagat ggcagcgatc cggacgcgtt
     1381 gtacaccatc gaacaccatc tttccgcaga cgatctggaa actctggaaa aagcggcagt
     1441 tgaagcgttt aaactcggtt acgaagtgac cgatccagaa gagctggaag ttgaagatgg
     1501 tgatatcgtg atttgctgcg acatcctcag cgagtgcgcg ttgaatgccg atctgatcga
     1561 tgcccaggtt gaacaactga tgacgctggc ggagaaattt gacgttgagt acgacggttg
     1621 gggcacttac tttgaagatc ctaacggcga agatggcgac gatgaagatt ttgtcgatga
     1681 agacgatgac ggggttcgcc actaattaat tgattaaaat gcccgatagc acatactagc
     1741 gggcattttt ttagagttcg cgtagcatcc gcacttcaca atcgacatgg cccgtgcagc
     1801 caagcgcata gtcgatatgc tcaaagccca aatgctcata aagcgcaatg gcttccttta
     1861 aaaaagcggt cgtttccaga tagcagcgtt tgaagcccat ctctcgtgcc tgctccatcg
     1921 ccattaaggc cagttttttc gctagccctt taccgcgaat agccggaaga aaatacatct
     1981 tttgcagttc gcaaatgtcc gactcactcc cggttaatgg cgcaatccca ccgccgccga
     2041 ccacttcacc ctcgtactca accacccaat atgcatggcc aggctgacta tacacttgat
     2101 acagctcgtc aagattcgga tcggcgacgg tgtagccttt atcagcggta agaccgtatt
     2161 cggcggatac ctgacgaatg acgcgggcga tagcaggatt atccagcagc gtcaggcgac
     2221 gcattacagg tgattgcggc gcaatgttat tcatagttta a
//
LOCUS       DAZPLM010000343         2246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPLM010000343 DAZPLM010000000
VERSION     DAZPLM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.346"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            117..641
                     /gene="aroL"
                     /locus_tag="ACHVC7_004302"
     CDS             117..641
                     /gene="aroL"
                     /locus_tag="ACHVC7_004302"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR2118166.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            691..882
                     /gene="yaiA"
                     /locus_tag="ACHVC7_004303"
     CDS             691..882
                     /gene="yaiA"
                     /locus_tag="ACHVC7_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR2118167.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1140..1817
                     /locus_tag="ACHVC7_004304"
     CDS             1140..1817
                     /locus_tag="ACHVC7_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR2118168.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHVC7_004305"
     CDS             1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHVC7_004305"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR2118169.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          595 a          505 c          561 g          585 t
ORIGIN      
        1 ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga tggtatgatc
       61 gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga
      121 cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc
      181 ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa tcacagctca
      241 atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc gccagagaaa
      301 cggcggcgct ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta
      361 ttctgacgga atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg
      421 cgccagtatc agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa
      481 ccttaacggg aaaaccgctg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc
      541 tatatcgcga agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt
      601 ctgaaattcg tagcgccctg gcacagacga tcaattgttg attttagagc gcctatactt
      661 aacgttcatc ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa
      721 gcatatatag tgacgattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc
      781 agagccgatc atcctaaacc agactcgttg atcagtgaac atccgacggc tcaggaagcg
      841 atggatgcga aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcgg
      901 aagggattct gagtgccact acaagggatc tgcgtcacat ttttcataat tcatgttttt
      961 ctaataatta gaatattaaa caataacaat ccattactgg aatcatttgg aatctttaca
     1021 ttatgccgtg cacgtctgct gctacgcttt ttgtcatttg tagcacaagt aagtgtcagc
     1081 agtggtgctt cacacttgcc cggtaattaa cgacgaaaga aaagtaaggt ggatgaacaa
     1141 tgagtgcgtc gttggcgatc ctgaccatcg gcattgtacc tatgcaggag gttttgccgc
     1201 tcctgacgga atacattgac gaagataata tttcccatca tagcctgctg gggaagttaa
     1261 gtcgtgaaga agtgatggcg gagtacgcgc cagaagcagg cgaagacacc attctcacat
     1321 tattaaatga caaccagctg gcccatgttt cgcgtcgtaa agtggagcga gacctgcaag
     1381 gtgtggttga agtgctcgat aatcagggtt atgacgtcat tttattaatg agtacagcaa
     1441 acattagtag tatgactgcg cgtaatacga tctttcttga gccgtcgcga atattgcctc
     1501 cactggtttc ctctattgtt gaagatcatc aggtgggggt tatcgttccg gttgaggaga
     1561 tgctgaccgt tcaggcgcaa aaatggcaaa ttttgcagaa atcgccggta ttttcattgg
     1621 gtaaccccat tcatgattca gaacaaaaaa tcattgatgc cgggaaagaa ttactggcaa
     1681 aaggggctga tgtcatcatg ctggattgtt taggatttca ccaacgtcat cgcgatttac
     1741 tgcaaaaaca gctcgatgtt cctgtcttgc tgtctaacgt attgattgca cggctggctg
     1801 cggaattact ggtgtaattt tgcgtgacag ccagcgcctc tggcccctat agtgaagtag
     1861 atgttcaact accagacagg gccagtttat gcttcaaagt aatgagtact tttccggcaa
     1921 agtgaaatca atcggctttt ccagcagcag cactggtcgc gccagcgtgg gtgttatggt
     1981 tgaaggcgaa tacaccttca gcaccgctga gccggaagag atgacggtaa tcagtggcgc
     2041 gctgaatgtg ttactgcctg acgcgaccga ctggcaggtg tatgaagccg gttcggtgtt
     2101 taatgttccc ggtcacagtg agtttcatct gcaagttgcc gaacccacct cttatctgtg
     2161 ccgctatctg taattcctcg ccttcccctt gaacgggagg gcatttttct gaaatatcct
     2221 ctctttaacc cataataata tttcct
//
LOCUS       DAZPLM010000344         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPLM010000344 DAZPLM010000000
VERSION     DAZPLM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.347"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(55..571)
                     /locus_tag="ACHVC7_004306"
                     /pseudo
     CDS             complement(55..571)
                     /locus_tag="ACHVC7_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(642..>2183)
                     /locus_tag="ACHVC7_004307"
     CDS             complement(642..>2183)
                     /locus_tag="ACHVC7_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFR2118170.1"
                     /translation="LLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVN
                     AWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIA
                     DIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDAS
                     GYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQS
                     AAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAAD
                     RIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPSAFSLTPDGKLTAK
                     NADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTE
                     TPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGV
                     LIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAAS
                     GISIR"
BASE COUNT          498 a          632 c          510 g          543 t
ORIGIN      
        1 caaaaaagcc cgcctgaacc ggcgggctgt cataacactg tgttacctgg ctaatcagaa
       61 tttataaccg acacccacga tgaaaccgtc agtgcgccag agacgtgttg ctgtggcgac
      121 cgtcatcact tccggtcagc acatcgtgcg ttttcccctt attgtcagtt acgcggatat
      181 aatccccgga aaaagtcgac acacggctgt aagccacacc cgccatcgca tacgcgctga
      241 accattcatt cacgcgcaca gacggccccg ccatcacgct gaaccagcgg ttacgcacgg
      301 aatcttcatg ccagcgggta tcgctgtaac gggtcagctg gcgattcttg tctcctgcat
      361 agctgaatga cgtcaccagc cccagcgtat ccgtaaactc ataacggtat ttcacgttaa
      421 tcccgttcag atcatcactg ccgggaacgt tggtccgggc atgaagatac cccgcgctca
      481 gcgtggactg atgttcagac gcccatgcag gcgcaccgga tacggccagg caaatggctg
      541 cggacaaaat ggctgcacaa actttacgca taattacctc tcgcttttct gcaataaaaa
      601 aggcgtcatt cctgacgcca tttattgggg ttataaatat ttcaacgaat actgatgccg
      661 gaagccgctt ttttggtcac aatcaccgta cagtcggtga tattgcctgc cccctgattg
      721 cctttctgga aaatcttaaa ctccagagtg acgctaccac caccactagg catatcaata
      781 actgcactgt aactaccggg aatggcacct ttagtttctc tggatgcgat taatacgccg
      841 tttttgcgaa cttcaaaacc ataacccgtg tatcgcgtgc ctcctgggtt atttccgctc
      901 cccggatcgt catacgctat accgttaaaa ataatgggcg gaataataat ctggcggtca
      961 aagttatgat catcgcttat ggtgactgta accgtcccgt ttggtgtttc cgtgttaccc
     1021 cacgtaccag cctgtttcgg aaatgatttg gatacagctt taacgaagtc acctctgacc
     1081 tgagtcgcct ccagcatgcc cttaatcgta cagttttcat ttaccgtgac attgttgagc
     1141 gtcccggcgt tcgcattcac actgccactg atatccgcat ttttagcggt cagctttccg
     1201 tccggagtca gggaaaatgc cgatggattt ccaccgctgg taatggtggg ggccgtcagg
     1261 cgtttcagga acacgtcgtt catgaatatc tgatcgccct gaccaacaaa catcggtttt
     1321 gtgttgccat tcgcaggatt aaccatcgca atcctgtccg ccgccagcag cacctgactc
     1381 tgcatgccgt caggggtgtt ctcaataccg gcaccaatac ccgcgatata aaggcgtccg
     1441 tcctgcatct gctgcagctt cacagcccac atgctgttca ggttattatt tgtatcaacc
     1501 tgaaccttct gtatctgctg aattgccgca ctctggtctt ccagtttctt attgacggtc
     1561 tgcgtgattt cattgctgac atccgtaatg gacgtcctga tttcagccag gtcaggcgca
     1621 agctgaccgt tatcaatctg cgtccacagc tcctgagcca gatgggtttt ccctatctcg
     1681 cctttgaaaa aatccagata accggatgca tcatcactcg gctgaccaac agcctccacg
     1741 aatgccgatt tgccaacggt gttcacactg cggatataaa agtaataatc atggcccggt
     1801 ttgatattga tactggcggc tatccagtac agcgccgtgc caagatagcg tgctgtggtt
     1861 tcaacctgcc tgatatccgc aatccgcttt tccgagaacc agaactcaaa ctgtaccgtc
     1921 ggatcataaa ccgcaagatg cggcgtggcg gttatctgaa aatagcccgg cgtcagctca
     1981 atccgcgaca gcgctgccgg tgcggcaatc cggaacgata ccgacgccgg atcgccctgc
     2041 tgcccccacg catttactgc ccggactgtc agcctgtagt tccccagcgc cagttgtgtg
     2101 aagcggtaag tggtttccgt cgtccgggcc gtgctgacca gccgctcact gccgtcatcc
     2161 gctgttacgg tcagacggag cag
//
LOCUS       DAZPLM010000345         2180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPLM010000345 DAZPLM010000000
VERSION     DAZPLM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.348"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            149..658
                     /locus_tag="ACHVC7_004308"
     CDS             149..658
                     /locus_tag="ACHVC7_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR2118171.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            655..2073
                     /gene="phrB"
                     /locus_tag="ACHVC7_004309"
     CDS             655..2073
                     /gene="phrB"
                     /locus_tag="ACHVC7_004309"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR2118172.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          532 a          536 c          581 g          531 t
ORIGIN      
        1 atgcagctcg ggaaatagta gaataattct cactcagatg aagttgataa tatttttacc
       61 aacaaaatac ttccccgcaa aaactctgcc ccataaaatt catttaccat cgtctacact
      121 tactccttta taccgttcag gagcagtaat gaatctacaa cgatttgatg acagcaccct
      181 aatccgtatc tttgcccttc atgagttaca tcgactgaaa gaacatggct taacgcgcgg
      241 ggcgcttctc gattatcaca gccgctataa actcgtcttt ctggcgcatt ctcagccgga
      301 gtaccgcaaa cttggcccgt tcgtggctga tattcaccag tggcaaaatc tggatgacta
      361 ttacaaccag taccgccaac gcgtagttgt tttgctttct caccccgcca acccgcgcga
      421 tcacaccaat gttttgatgc acgttcaggg ttattttcgc ccgcatattg attccacaga
      481 acgccagcag ctggctgcgc ttatcgacag ttatcgccgt ggcgagcaac cacttcttgc
      541 gccgctgatg cgtatcaaac actatatggc gctttatcat gacgcctggc tttcagggca
      601 gcgttatttc gaactttggc cgcgtgtgat taacttgcgc cattcaggag ttttatgact
      661 acccatctgg tctggtttag ccaagattta cgtctgcacg ataatctcgc actggctgcc
      721 gcctgccgca attcgtctgc acgcgtgctg gcgttgtata tcgctacacc acgccagtgg
      781 gcgacgcata acatgtcgcc gcgtcaggct gaacttatca atgctcaact gaatgggcta
      841 caaatagcgc ttgcggaaaa aggtattcct ttattgttcc gtgaagtgga tgactttgtc
      901 gccagtgtcg aaatagttaa acaggtgtgc gcggaaaaca gcgttaccca cctgttttat
      961 aactatcagt atgaagtgaa tgagcgggcg cgggatgtgg aagttgaaag agcgctgcgt
     1021 aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc cgcctggcgc ggtgatgacc
     1081 ggtaatcacg agatgtacaa agtctttacg ccttttaaga atgcctggct gaaacggctg
     1141 cgggaaggga tgccggagtg cgtcgctgcg ccaaaagttc gtagtagcgg atcgatagag
     1201 ccctcgccat ccattacgct gaattatcct cgtcagtctt tcgatactgc gcattttccg
     1261 gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt gccagaacgg tgccggagaa
     1321 tatgagcaac aacgagattt tccggcagtg gaaggcacca gccgtttgtc ggccagcctg
     1381 gcaacgggcg ggttatcgcc tcgccagtgc ttgcatcgct tgttggctga acagccgcag
     1441 gcgctggacg gtggggtcgg tagtgtctgg cttaatgagc tgatctggcg cgagttttac
     1501 cgtcacctga taacgtatca cccctcgttg tgtaaacatc gtccaattat tgcctggacg
     1561 gatcgtgtac agtggcagag caatcccgca catttacagg cctggcagga aggcaaaacg
     1621 ggatacccga ttgttgatgc cgctatgcgt cagcttaaca gcactggctg gatgcataac
     1681 aggctacgga tgattacagc cagttttctg gtgaaagatt tattgatcga ctggcgcgaa
     1741 ggcgagcgat atttcatgtc gcagctgatt gatggtgatt tggcagccaa taacggtggc
     1801 tggcagtggg ccgcttcaac cggaaccgat gcagcgccgt attttcgtat tttcaacccg
     1861 acaacccagg gcgagaaatt tgatctcgaa ggtgagttta tccgccagtg gctaccggaa
     1921 ctacgcaatg taccggggaa atcggtgcat gagccgtgga agtgggcgca gaaagcaggt
     1981 gtgaagctgg attatccgca accgatagtc gagcacaaag aggcgagagt acaaacgttg
     2041 gcagcgtatg aggcggcgcg gaaggggaaa taacagacgc gtcaggcaat cgagcccaga
     2101 tgccggatgc ggcgtgaacg ccttatccag cctacaaatt tgtgcaaatt caataaattg
     2161 caggaaatac gtaggcctga
//
LOCUS       DAZPLM010000346         2165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPLM010000346 DAZPLM010000000
VERSION     DAZPLM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.349"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHVC7_004310"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHVC7_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR2118173.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHVC7_004311"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHVC7_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR2118174.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHVC7_004312"
     CDS             902..1549
                     /locus_tag="ACHVC7_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR2118175.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="ACHVC7_004313"
     CDS             complement(1591..2121)
                     /locus_tag="ACHVC7_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR2118176.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          639 a          420 c          487 g          619 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatatgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa caatgacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcaatt
     2161 tgcca
//
LOCUS       DAZPLM010000347         2135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPLM010000347 DAZPLM010000000
VERSION     DAZPLM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.350"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            154..>2135
                     /gene="yjcS"
                     /locus_tag="ACHVC7_004314"
     CDS             154..>2135
                     /gene="yjcS"
                     /locus_tag="ACHVC7_004314"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR2118177.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTP"
BASE COUNT          572 a          565 c          522 g          476 t
ORIGIN      
        1 cctcttcagc acaaatgcct aataaaatgg atcgttcagc tcacacttcc acttattaaa
       61 gtcgcaaatt cccaattatg ataaccccag ctttaaatga cgtattttgc atggattaca
      121 tatttacgac ataaatgata aatggagatt tacatgaata actctcggtt attccgtttg
      181 agcagaattg ttattgcgtt aactgccgcc agcggcatga tggtaaatac cgctaacgcg
      241 aaagaggaag cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa
      301 tcattaccgt ttagcgatcg tcaggatttt gacgacgctc agcgtggatt tatcgctccg
      361 ctgctggatg aaggtattct gcgcgatgct aatggcaaac catattatcg cggggaagat
      421 tataaatttg atatcaatgc gcccgcgccc gaaaccgtta accctagcct gtggcgtcag
      481 tcgcagttaa acggtatttc cggcctgttt aaagtcaccg acagaatgta ccaggttcgc
      541 ggtcaggata tctccaacat cacctttatc gaaggtgata ccgggattat tgtcatcgac
      601 ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg
      661 caaaaaccga ttgttgcggt tatttatacc cacagccata ccgaccacta tggcggcgtg
      721 aaaggcatta tctctgaagc tgatgtgaaa tccggtaaag tgcaggttat cgcccctgct
      781 ggctttatgg acgaagccat cagcgaaaac gtactggcgg gtaatattat gagccgccgt
      841 gcactttact cctacggcct gctgctggcg cataaccctc agggtaacat cggcaatggt
      901 cttggcgtaa cgctggcatc gggctacccg agcatcatcg caccgaacaa aaccatcacc
      961 aaaaccggtg agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt
     1021 agcgaagcac cagccgaaat gcacttctat attccggccc tgaaagcgct gtgtaccgcc
     1081 gagaacgcca cgcataccct gcacaacttc tacactctgc gcggcgcgaa aacccgcgac
     1141 accagcaagt ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa
     1201 gtcctgttta tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt
     1261 ggtaaatatc gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag
     1321 ggctacacca tgaatgaaat cggcgacatg attaaactgc cgcctgcact tgccaataac
     1381 tgggccagcc gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc
     1441 tatcttggct attacgacgg taacccggct aacctgcatc cgtatggtca ggtggagatg
     1501 ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa
     1561 gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc
     1621 aacccgggtg accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat
     1681 caggccgagt ccgccacctg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa
     1741 ggggtgcata agttcagcca cggcaccatc ggttccccgg acaccattcg cgggatgtcg
     1801 gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa
     1861 aatatcagcc tgaacttcaa tatgagcaat ggcgataacc tcaacctgac gctgaacgat
     1921 agcgtgctta actaccgtaa aacactgcaa tcccaagctg acgcctcttt ctacatcagc
     1981 cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa tggcggatct ggtaaaagcg
     2041 aagaaagcca aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat
     2101 aatttcgatt tgtgggtgaa tatcgtaacc ccaaa
//
LOCUS       DAZPLM010000348         2117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPLM010000348 DAZPLM010000000
VERSION     DAZPLM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.351"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..963
                     /locus_tag="ACHVC7_004315"
                     /pseudo
     CDS             1..963
                     /locus_tag="ACHVC7_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            974..1411
                     /locus_tag="ACHVC7_004316"
                     /pseudo
     CDS             974..1411
                     /locus_tag="ACHVC7_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1411..1788
                     /locus_tag="ACHVC7_004317"
     CDS             1411..1788
                     /locus_tag="ACHVC7_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118178.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          701 a          330 c          523 g          563 t
ORIGIN      
        1 aagcaatatg gtgattttga aaatggtatt cctgttcacg ataccattgc cagagttgta
       61 tcctgtatca gtcctgcaaa atttcacgag tgctttatta actgaatgcg tgactgccat
      121 tcttcagatg ataaagacgt cattgcaatt gatggaaaaa cgctccggca ctcttatgac
      181 aagagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat gcacagtctg
      241 gtcatcggac agatcaagac ggatgagaaa tctaatgaga ttacagctat cccagaactt
      301 cttaacatgc tagatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
      361 gatattgcag agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac
      421 caggggcggc taaataaagc ctttgaggaa aaatttccgc tgaaagaatt aaataatcca
      481 gagcatgaca gttacgcaat gagtgaaaag agtcacggca gagaagaaat ccgtcttcat
      541 attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa aggactgaag
      601 aaattatgcg tggcagtctc ctttcggtca ataatagcag aacaaaagaa agagccagaa
      661 atgacggtca gatattatat cagttctgct gatttaaccg cagaaaagtt cgccacagca
      721 atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat
      781 gaagacgact gcaaaataag aagaggaaac gccgcagaat tattttcagg gatacggcac
      841 atcgctatta atattttaac gaatgataag gtattcaagg cagggttaag acgtaagatg
      901 cgaaaagcag ccatggatag aaactatctc gcgtcagtcc ttgcggggag cgggctttcg
      961 taatctttcc ctagggagtc agccgaatgt tgggaaggat ttgacggatg aagagaagaa
     1021 ctgcttggcc ggcggcagta cgggaagccc gggaggatgg ggaccagaag atgagaatag
     1081 cgcaagggat cgtatgcagc atgaacggta taaagatgaa ttgcgacaga aaatgggaaa
     1141 acccgacgtt acagattcaa atttgaaaaa tattatagac gatctttatc ggccaaatgc
     1201 aaaagtagga agtggaagca cagcagatgc tgttcgttat gaattggcca caggtgaaaa
     1261 agttggtggt agaggacatg tattgaaagc acaggattat acaagggctt tacaggattg
     1321 gttggataag aatccaacag catctccagg ggatcgagca gcagcagaaa acgttcttca
     1381 ggatttacag aatgctttgg gaggcaagta atgaaccaat atattgctag ttatatagaa
     1441 aaaatggcat cattttccac tgttttgtct gagacagtct cattagctaa tgaatactgg
     1501 catccagaaa cgccaccggt ggttatgttg ttctctcagg ttggaaaaac gattgtaaaa
     1561 aatttttatg aaatggggga tattgaaaga gaaaaaatat ttaaacatgt cgaagatggt
     1621 atggtctcgc ttgatgatga attggccaca gttgtagcta cagggcttat agaggcaata
     1681 gtcactgcga ccgatagcaa ttctttactt tggcaagatt taagtaaatt tctgggtgac
     1741 aaatcaaagt ctcatgcatt agtgtggaaa aattttggtc aggagtgata ttattcaaga
     1801 agtattaagc tcgctattga gatcttaatg cttcttgaga ttaaattaaa gttaggacga
     1861 agtaattgca ggtggaaata aatccatagt gacgtgtggc aatggctatt agatgttatg
     1921 gtgtgatggg ctttggtgat ttaagagcta agttagattg ccttatgaaa aagtatggtt
     1981 atatatttaa tgatgtgtat catatattta aataaaaaac ggttctctct tgtcaagata
     2041 gtaataggat agactattgt gttgataaat ataattaaga gtctactttg ttgattttag
     2101 aagactttgg agcaggg
//
LOCUS       DAZPLM010000349         2093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPLM010000349 DAZPLM010000000
VERSION     DAZPLM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.352"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHVC7_004318"
     CDS             complement(<1..123)
                     /locus_tag="ACHVC7_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR2118179.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHVC7_004319"
     CDS             384..719
                     /locus_tag="ACHVC7_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR2118180.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHVC7_004320"
     CDS             complement(1000..1131)
                     /locus_tag="ACHVC7_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR2118181.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHVC7_004321"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHVC7_004321"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHVC7_004322"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHVC7_004322"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="ACHVC7_004323"
     CDS             complement(1986..>2093)
                     /locus_tag="ACHVC7_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2118182.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPLM010000350         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPLM010000350 DAZPLM010000000
VERSION     DAZPLM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.353"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHVC7_004324"
     CDS             complement(60..1025)
                     /locus_tag="ACHVC7_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR2118183.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHVC7_004325"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHVC7_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118184.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPLM010000351         2077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPLM010000351 DAZPLM010000000
VERSION     DAZPLM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.354"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHVC7_004326"
     CDS             complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHVC7_004326"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR2118185.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            1409..1879
                     /gene="argR"
                     /locus_tag="ACHVC7_004327"
     CDS             1409..1879
                     /gene="argR"
                     /locus_tag="ACHVC7_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR2118186.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          568 a          527 c          442 g          540 t
ORIGIN      
        1 gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc
       61 aaggcgatat ctttcttgag tgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
      121 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
      181 gagaagaaac gggcgtactg accatcgcct tcaacgtagg cacattcgac aacgccttgt
      241 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
      301 agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacccgc gttctggatg
      361 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
      421 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
      481 tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatgtccag cgtggtaacg
      541 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
      601 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
      661 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
      721 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
      781 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
      841 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
      901 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
      961 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     1021 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     1081 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     1141 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
     1201 cagccaaaac aacatcaatt tgataacaat taatttactt ttaaacagaa tttgcatgcc
     1261 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     1321 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     1381 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
     1441 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
     1501 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
     1561 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
     1621 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta
     1681 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
     1741 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
     1801 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct
     1861 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
     1921 attcctctca accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttaccgc
     1981 gacatatcat tcacacaatg aatacatacg gtaaaaaaag cacattatgt aaaattcatt
     2041 atctaattga aaatactaga attaacaata aataacc
//
LOCUS       DAZPLM010000352         2030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPLM010000352 DAZPLM010000000
VERSION     DAZPLM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.355"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHVC7_004328"
     CDS             <1..314
                     /locus_tag="ACHVC7_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118187.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHVC7_004329"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHVC7_004329"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR2118188.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          492 a          500 c          479 g          559 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa
//
LOCUS       DAZPLM010000353         2022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPLM010000353 DAZPLM010000000
VERSION     DAZPLM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.356"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(271..1275)
                     /locus_tag="ACHVC7_004330"
                     /pseudo
     CDS             complement(271..1275)
                     /locus_tag="ACHVC7_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1260..1637
                     /locus_tag="ACHVC7_004331"
                     /pseudo
     CDS             1260..1637
                     /locus_tag="ACHVC7_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1676..>2022)
                     /locus_tag="ACHVC7_004332"
     CDS             complement(1676..>2022)
                     /locus_tag="ACHVC7_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2118189.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          468 a          483 c          553 g          518 t
ORIGIN      
        1 ataatgataa tcactgtcag ttatatttgt atgacagatg taacaaaatg aaaaaaaaag
       61 ataagcacaa gtgattgatt ggtcagatat tgatcacttg gtgtttgtat tttgaacttt
      121 gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt tacaggtttt ctgtattatc
      181 cgggggagtt aagtctgtcg atgtccgaca gacttttgat gatggaaaca acatgcagcg
      241 ataatgtgca tgctgttttg caggtggaac tcagaaggtg aactggaact gtgcgaccgc
      301 accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca aggtgaacca catcaaacgg
      361 tgagatcccc accccggccg tgaatgcact gccgtcgtta ccggccagat tctgacggta
      421 accggcgcgc agctgtgccc atttccaggc attaaattct gcgccaatgg cggcaaactg
      481 acggttgctg tcggaggtga aaccgctggc cggggtcaga tccacatcaa atgcggtggt
      541 gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc gggcggatcc tgaaggtttc
      601 cgtgataccg ttcacttctt ttgtctcaat actacggggg atcaggttct gtgcgaccag
      661 tcccagcgtc cagttgtcat ccaggtccgt actggcaccg atgtcggcgt tgatgccatt
      721 tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg tcataatttc tgaccagcac
      781 gttgtagttg aacaggtcga cgcgctggaa tttggggggg aagcccagag accatttctg
      841 cccggccgtt tccagttccg tagccagagc aatacccaca tcagtgatca gtgccgcgcg
      901 ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc tgcccggtgc tgtttgccac
      961 ctcttccagg taagtgaggt cagcatcaga gactttaccg tcgacactga cggtgccgtg
     1021 tgatttaacc atcagtgcgg cagagaggcg atcaccggga agggccgcca cagcagaaac
     1081 gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg aggtgttctt tcaggcgtgc
     1141 ggcagcctca ggaacaccgt ggttactttc gatggcgcgg tcgaaggcct tccactcatc
     1201 actgatccgg tcggcgttgt cggtgatatt atccggattg gagagctgcg cgccgacgga
     1261 cggaagcagg atgctgggct catgcgcgac gcaaaatcca cgatgtatat atcagtacta
     1321 aaagcgcgac ggcggaagaa gctctgaaac taatcggtga actgtacgcc atagagcacg
     1381 atatacgcgg gttaccggtg tctgaacgcc tggcggtcag gcaaatgcag agtagaccgc
     1441 tcctgacttc cctgtataag ctgatgcagg agaaagaaca cacgttatcg aagaaatgtc
     1501 gtctgggaga tgcgttccgg tatatcagga agcactgggc tgcgttgtgc aacttctgtg
     1561 atgatggcct ggctgaggta gataataatg cagcagaaag agcgcttcgt gcagtctgtc
     1621 tcgggaagaa aactttttcg aggatcactc attccgtgtc tgactgcttc agggtttaat
     1681 tttgcttctg ctgactgaat gatgattggg cagcaagtgc aatatcccac ataaatccag
     1741 ttaactcacg agccaccgcc gtaatggcta cttgtgattt tttccctttt gctgtcagag
     1801 ttcggtaacg agaacacaat cgttgttgag ctttccatgc aatatctttg gaatattgtg
     1861 ttacagagtc cggtcggtaa tatataagcc atgctccaac ttttgcaggt gtacgataag
     1921 accaagcggc ttcgtaaagc agacgtctca gttcgctgtt tccaactttt gttattcctc
     1981 tgggacgaat actgtttcca ctggaatatt cacctggcac ca
//
LOCUS       DAZPLM010000354         2020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPLM010000354 DAZPLM010000000
VERSION     DAZPLM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.357"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..356
                     /locus_tag="ACHVC7_004333"
     CDS             <1..356
                     /locus_tag="ACHVC7_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFR2118190.1"
                     /translation="IDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLN
                     GKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPF
                     GQTILRKVTFSNIES"
     gene            393..2020
                     /locus_tag="ACHVC7_004334"
                     /pseudo
     CDS             393..2020
                     /locus_tag="ACHVC7_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          635 a          380 c          399 g          606 t
ORIGIN      
        1 tgattgacat taaatttata gctgatatac agtttaatac tgacttatgt ggtgacactt
       61 atatttccag aggtcccgtt ttatatgcaa ttgagcttga gagcgatatt ttaatcaaaa
      121 aaaacctgct gaacgggaaa tattttgact caggatacat accctgtagt agagctgatg
      181 aatctatcca gttctcttat gctgatatgg aatctttttc atattcaacc tcaccagagg
      241 gcaaacaata cattgaaggc tgtttttatt taaacaagac taaaatagtc agaaaactta
      301 taccatttgg tcaaaccatt cttcgcaaag taactttctc caatatagag agttaatatt
      361 ttcgagtgtt ataattaatt aaagggattt ctatgtacct tactgataat attatcaaac
      421 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgaagcat
      481 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      541 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      601 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      661 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      721 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      781 acgaggttga gttttggatt gatgctgcct gcaacgattt atttggctat gttactaatg
      841 gagggattat tactgatgtt catatcgcga cacgtaatca actactgaaa tccctttatt
      901 atgatattga ggtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      961 ctaaaggtat caccccagaa caatttattg ccaaaagaag tgagcgtact gacgaaatca
     1021 taagtactct cgaatatatt gataatacgt taattacctt tagcaatgaa gaaataatta
     1081 aatgtcaaat tgcaatccaa tccataatca ataaaaacaa taatccttca gagttcagga
     1141 ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta agagaaggac
     1201 gcagaaaagc aataagaaca tttgccaccg cccttgcaaa cattgaaaaa tatcctgatt
     1261 acatttttgg cgctagtcag taccaacttt ttcactggat aaaaaaagat tatccctatt
     1321 tcttcgaaaa gttaaaaaaa caaatcgccg caggccgctt tgagttacaa ggttgcttct
     1381 gggtcgaatg cgatttaaat ctggtaagtg gtgaatcgct gataaggcaa atcatgcatg
     1441 ggacacgatt cacactgcag aatttttcaa aaaagataaa ttatgtctgg caaccggatg
     1501 tctttggatt ccccgcaacg cttccacaaa ttctcaaaaa atcaggcatt aactatattg
     1561 catcgcaaaa gctctcacaa aacaagatta ataaatttaa taattacctt ttccgctggc
     1621 agggcctgga tggctcagaa attctgatgc ataactttcc tgaagacact tatgatagcc
     1681 gagcacgtgc tcgctcttta gaatatattg aacaaaacta taatgaaaaa gagatctgtc
     1741 cctatgcgct tatggtgtat ggtgtatggt gtcggtgatg gcggcgctgg ccccggtgag
     1801 gaacatatag aacgattaac acgtatacgc aatattgatg gcttacctca tgttgatttc
     1861 agccgtgttg ataagttctt cacttacgcc gacgcctttc gcgaaagctt gcccattatt
     1921 tcaggtgaac tttatttcga ggctcatcaa gggtgcttta catcagagtc agcaactaaa
     1981 gctcataatc gaaatatgga aaataaatta catgatgctg
//
LOCUS       DAZPLM010000355         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPLM010000355 DAZPLM010000000
VERSION     DAZPLM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.358"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHVC7_004335"
     CDS             <1..389
                     /locus_tag="ACHVC7_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118191.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHVC7_004336"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHVC7_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR2118192.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHVC7_004337"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHVC7_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR2118193.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHVC7_004338"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHVC7_004338"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHVC7_004339"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHVC7_004339"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPLM010000356         1973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPLM010000356 DAZPLM010000000
VERSION     DAZPLM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.359"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHVC7_004340"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHVC7_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR2118194.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHVC7_004341"
     CDS             complement(390..1178)
                     /locus_tag="ACHVC7_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR2118195.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHVC7_004342"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHVC7_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR2118196.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHVC7_004343"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHVC7_004343"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR2118197.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          500 a          523 c          454 g          496 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta cac
//
LOCUS       DAZPLM010000357         1927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPLM010000357 DAZPLM010000000
VERSION     DAZPLM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.360"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHVC7_004344"
     CDS             complement(<1..271)
                     /locus_tag="ACHVC7_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2118198.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..854)
                     /locus_tag="ACHVC7_004345"
     CDS             complement(315..854)
                     /locus_tag="ACHVC7_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR2118199.1"
                     /translation="MDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVL
                     KPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTA
                     VRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIGNKQEEIN
                     EDTEESTTDDTTAEDTENE"
     gene            890..1453
                     /locus_tag="ACHVC7_004346"
     CDS             890..1453
                     /locus_tag="ACHVC7_004346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118200.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1599..>1927)
                     /locus_tag="ACHVC7_004347"
     CDS             complement(1599..>1927)
                     /locus_tag="ACHVC7_004347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118201.1"
                     /translation="KSFALVSLMEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMS
                     FYVTRYLGKMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDD
                     IKCKRL"
BASE COUNT          577 a          353 c          331 g          666 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccta ttacttccat gtcatcgggt
      421 aactcaaatt ccattgagcc taattggtat aatgttttat caatatgcgt tcttaataca
      481 tcgcgtaaat cctttgcatc tgattgtgca aataattcaa gataagtttt tgatggtatt
      541 gcacgcactg ctgttttgac ctggaataga tattccgtta gtacctgttc tatatattca
      601 gtactgacca ccagcccatt cttttctgct aattctaatt ctgctaactg ccgttctgct
      661 gaaagcttct tcaaacgctc ttgttcaatt tgttcttttg tattagtatc acgcaacggc
      721 tttagtacat tatctacaat ccaacttctt gcgtttgaat cagtgtctgt cggcattccc
      781 ttttctaccc aagttcgaac tgtagattca tcgtatccgt actgtctcgc taatgacctt
      841 aatgagatat ccatatttgt tcctttttat tttatttata gggtttacaa tgtcagaccg
      901 taacaatgat ttttctctgt tagtaaaatt gctgaacgga tttttgaaga aagatgagat
      961 aaaaaaaaga ctatattctt tcgtgaaaaa agaaactaat gattgggaaa agtggctgca
     1021 aatagaattt gctcattatc taacttcaaa aaggaagtac gagctacatc gtgaagttac
     1081 gatatacctc gatccaatag attatcctga tagtaaactt gctaaagtag atttgatact
     1141 gcgtgaaaaa tccacaccct ctgatgatta tatttttatt gagtttaaat gtactaaagg
     1201 tgtagtacca ttagttaggg gcttaaatgc tgacaaaaat aaaattagtg ctataagaca
     1261 gtgtaactac aaaatgcgta attatcttgg tgtcggattt catctttttt gtgaaccaga
     1321 tgatatcgag tttatggaaa agtacgtaac tgaagaactt aaaggctctt acgccgtgtt
     1381 taaattatgc gagtgtccag taagatataa ttgccaatgt gaatttaatg aaattggtgt
     1441 ggtacttttt taaatgcggt gcgggtatga tcattttgtt cacacacaat tgattaaacc
     1501 cgctgccgaa aaccctcgat agtaccgcca gtatggaggg acccattgaa tgcaatggtt
     1561 aatatacttt gtaagctata tacaatgaat catgagtatc aaagccgctt acatttaata
     1621 tcatcgtaca aagttccttc tttgtttaga tatctcgaac cgtaattata tccatcatca
     1681 aatgcttcat tggttattac cagttccctg taaaatttct catcaggtgc tgtttcataa
     1741 tgaaatgcac cataaggcgg cggcatttta ccgagatatc ttgtaacgta gaaactcatg
     1801 tcagtactaa atgaatcgtt atgaatatcc cagcatttaa agtctggaat agctgcactt
     1861 gttgttaaag gtaataataa tagcaatatc aatttttcca taagagatac caatgcaaat
     1921 gatttaa
//
LOCUS       DAZPLM010000358         1893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPLM010000358 DAZPLM010000000
VERSION     DAZPLM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.361"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHVC7_004348"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHVC7_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR2118202.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHVC7_004349"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHVC7_004349"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR2118203.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHVC7_004350"
                     /pseudo
     CDS             complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHVC7_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
BASE COUNT          543 a          447 c          368 g          535 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tca
//
LOCUS       DAZPLM010000359         1806 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPLM010000359 DAZPLM010000000
VERSION     DAZPLM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.362"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..724)
                     /locus_tag="ACHVC7_004351"
     CDS             complement(<1..724)
                     /locus_tag="ACHVC7_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2118204.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEI"
     gene            complement(732..1127)
                     /locus_tag="ACHVC7_004352"
     CDS             complement(732..1127)
                     /locus_tag="ACHVC7_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR2118205.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            complement(1124..1702)
                     /locus_tag="ACHVC7_004353"
     CDS             complement(1124..1702)
                     /locus_tag="ACHVC7_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2118206.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1714..>1806)
                     /locus_tag="ACHVC7_004354"
     CDS             complement(1714..>1806)
                     /locus_tag="ACHVC7_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR2118207.1"
                     /translation="FWVDRVSPDDGGSCHLWLGRGVPPAVNRRR"
BASE COUNT          375 a          574 c          445 g          412 t
ORIGIN      
        1 tgatttctgc aaccgcagca aactcaccat taccagatac gaccggaatg ttgaccttgc
       61 ctgcagcaac acctttcacg gtgatggtca taccactgac cgacacggtg gcttttgttt
      121 tatccgcaga caccgcacgg aagctcttgt cggttgcgcc atccggctgg aatgccacgg
      181 tcagcgtggt gctctgccct ttcactacgg aagcactggc gggtgtcacc gtcatgccgg
      241 tcgtcgccgt caccgtgctg cgatcttctg ccatcgacgg acgtcccaca ttggtgacct
      301 tcaccgtgcg ggtaatcacc tccttcgccg tcaccgcctt accgatactg ctgacccagc
      361 cacggaacac atcgaccgtg ccgttcggga agcggatttt ataggcacgg gtatcacctt
      421 cattaaacca cgccagcagc gcctgctgcc cctgctctcc gggcatccac gccagcgtga
      481 agctggtatc tccggcagat ttctgcccct gcccggtcgc agtccagtct gcatcttcat
      541 catcgagata gctgtcgtca taggactcag cggtcagttc gccgggcgtc aggtctttaa
      601 cttttgccag acgcgaccag tcaacgtctg aaagcgggtt cgcataaggg tcaccgctcc
      661 ccttataaac ccacagggtg gtcccggcac ctttcaccgg cattgtagga tttggtacag
      721 gcatagcgtc ctcacatttc atagataatg acataagtca gatcggctga actccacagg
      781 cccgcatcat cgtcgcgccg gtagtcatag ccgctggcca ccatactggt gatcaaatct
      841 gacagtgccg ggatatcgct catcaccgga taaatccggg actccatcca cgaatccagc
      901 tctgaatccg gcacctgagc aggcaggaaa acttcaatat gcagctccgc ctgccaggta
      961 tcgctgtcca gctcttcgcc cgtgtattca gcgccggtga gataaacggc aattgccgga
     1021 aaatcttcct catcaaaaac agcggggcga ccatcaaaaa gcgtcgcccc ggtgtcatgc
     1081 ttctccagtg catccagtac ggctgcacgg agttcagtat gtttcatcgc tttattacca
     1141 ttctcagttg atgctgcagc gcatagccca gctctttcgg aagacgttca cgccgtatcc
     1201 gttcaatatt ctgtttaaac gccgtggtca gcggcaccgc catcgggatt ttcaccacat
     1261 caatggggta acggtttttc ccggccacac gctgcataac atgccaccgg ccgtttttca
     1321 gttgctgaat aaacgcgccg ggaatacgac ggttacccac cacaagcacg ctgccgccac
     1381 ctttcaggga tgaacgctgc ccctttttat gacgcctgcg gcgggacagg acaacccgcg
     1441 cattacccag cttgattacg ggcaaatccc cccggttaac tttgattctg gcctgcggat
     1501 ttttgaccgt ggcccttttc agcctggccc tttcctttac cagtttccgg cgtacctttg
     1561 tctcacgggc aacctgtgac gcagactgcg atatcgcgga tgaagcaacg cggttaatgg
     1621 ccattgcggc ggcaccgggc accgccgttt tgctgatacg gctgaggttt tcaacggcct
     1681 gctcaagacc ttttatggcc atacatcccc ctttcagcgg cgacggttaa cggcaggcgg
     1741 tacgccccgt ccaagccaga gatgacaact tccgccatca tccggcgaaa cccgatctac
     1801 ccagaa
//
LOCUS       DAZPLM010000360         1788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPLM010000360 DAZPLM010000000
VERSION     DAZPLM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.363"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..483
                     /gene="cas6e"
                     /locus_tag="ACHVC7_004355"
     CDS             <1..483
                     /gene="cas6e"
                     /locus_tag="ACHVC7_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR2118208.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
     gene            480..1403
                     /gene="cas1e"
                     /locus_tag="ACHVC7_004356"
                     /pseudo
     CDS             480..1403
                     /gene="cas1e"
                     /locus_tag="ACHVC7_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHVC7_004357"
     CDS             1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHVC7_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR2118209.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT          432 a          398 c          511 g          447 t
ORIGIN      
        1 cgcttttttg tcctgtcgca ggaacgcccg gcggaaagtg atattttcac catcgaatgc
       61 cgaccatttg cgccagagtt gcgtaccgga caatcccttt gtttcaacct gcgggcgaat
      121 ccaacaattt gtaaagcggg caaacgccac gacttgctga tggaggcgaa acggcaggtg
      181 agggggcagg tggaaggaag tgatgtctgg ttgcatcaac aacaggctgc gctggactgg
      241 ctggcagcgc agggggagag aagcggtttt acactgctgg atacttcggt tgatgcctac
      301 cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag tagcgtggat
      361 tatacgggga tgcttacggt caccgctcct gggttatttc tgcaacgtct cagtcagggg
      421 tatggcaaaa gccgggcgtt cggttgtggt ctgatgctga tcaaacccgg agcagaggcg
      481 tgacgtttgt accgctgagc ccgatcccat taaaagatcg cacttccatg atcttcctcc
      541 agtacggtca aatcgacgta ctggacggcg ctttcgtgct gatcgacaaa accgggatcc
      601 gcacgcatat tccagtggga tcggtcgcct gcattatgct cgaaccggga acaagagttt
      661 cccacgcggc agtccatctg gccgccacgg tgggaacact gctggtctgg gtcggtgaag
      721 cgggcgttcg cgtttactcc tccggacaac ccggaggggc gcgggcagat aaattactct
      781 actaggcaaa gctggcttta acggaagatc tacggttgaa ggtggtgcgc aaaatgtatg
      841 aattacgttt tcgtgaacca ccgccagctc gccgttcagt ggagcagtta cgtggaattg
      901 agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt gtgaaatgga
      961 atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat cgctgcatca
     1021 gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc gcgggatatg
     1081 cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat gacatagccg
     1141 atatcattaa atttgattcg gttgtgccaa aggcatttga aatagcagcg aggcaacccg
     1201 cagaacctga taaagaagtc agattagcct gtcgcgatat tttccgtagc actaagttaa
     1261 cgggcaaatt aataccgtta attgaggaag tccttgctgc aggtgaaatt gaaccaccac
     1321 aacccgcgcc ggatatgtta ccgcctgcca tccctgaacc tgaaacgctg ggtgatagtg
     1381 gtcaccgggg gcgcggcgga tgagcatggt cgtggttgtt acagaaaatg tccctccgcg
     1441 cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt atgttggtga
     1501 tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg ctggttgcgg
     1561 aaatgtggtg atggcctggg cgaccaatat cgagtcgggt tttgaatttc agacctgggg
     1621 agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt ttcttcctgt
     1681 tgataatcaa taagttatcc gttctttaaa aataaggaaa tgttttaatt tagttggtag
     1741 attgttgatg cggaataaat ttgtttaaaa acagttatgt atgcttag
//
LOCUS       DAZPLM010000361         1745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPLM010000361 DAZPLM010000000
VERSION     DAZPLM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.364"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>1745
                     /locus_tag="ACHVC7_004358"
     CDS             <1..>1745
                     /locus_tag="ACHVC7_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR2118210.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPN"
BASE COUNT          603 a          344 c          329 g          469 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaac
//
LOCUS       DAZPLM010000362         1740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPLM010000362 DAZPLM010000000
VERSION     DAZPLM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.365"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            201..965
                     /gene="nfeF"
                     /locus_tag="ACHVC7_004359"
                     /gene_synonym="yqjH"
     CDS             201..965
                     /gene="nfeF"
                     /locus_tag="ACHVC7_004359"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR2118211.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(1019..1094)
                     /locus_tag="ACHVC7_004360"
     tRNA            complement(1019..1094)
                     /locus_tag="ACHVC7_004360"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1059..1061),aa:Ile,seq:cat)
     gene            1219..1725
                     /gene="mug"
                     /locus_tag="ACHVC7_004361"
     CDS             1219..1725
                     /gene="mug"
                     /locus_tag="ACHVC7_004361"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR2118212.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          419 a          428 c          475 g          418 t
ORIGIN      
        1 actcatctca cacgtaggcc ggataaggcg ttcacgccgc atccggcgtt gagtaatgat
       61 gcctgagaag aggcggttcg tcttatcaga ctatagacat atcagttcta caaatcgctt
      121 gcatttatca tgattaacaa tcattatcat ttacgaaaat tatttagata tatctgatta
      181 ccatcacgaa ggcgataaca atgaataact ccccccgcta cccgcagcgc gttcgcaatg
      241 atctgcgctt ccgtgaactg actgtgttac gcgttgagcg catcagcgcc ggttttcagc
      301 gcattgtcct cggcggcgag gcgctggacg gttttacatc gcgcggcttt gacgatcaca
      361 gcaaactctt ctttcctcaa cctgacgctc actttgtgcc gccaacggta acggaagagg
      421 gcatcgtctg gccggaagga ccacgcccac cgtcgcgtga ctatacgccg ctgtatgacg
      481 aactacgcca tgaactggcg atcgatttct ttattcacga cggtggggtc gccagcggct
      541 gggcgatgca ggcgcaaccg ggcgataaac ttacggttgc aggtccgcgc ggttcgctgg
      601 tggtgccgga agattacgcg tatcagctgt atgtctgcga tgaatccgga atgcccgcac
      661 tgcgccgccg cttggaaacg ttgagcaaac ttgccgttaa accgcaagtt agtgcgctgg
      721 ttagcgtgcg ggataacgcc tgtcaggatt atctcgcgca ccttgatggt tttaatatcg
      781 aatggctggc acatgatgag caggcggtag atgcgcgtct ggcgcagatg caaatccctg
      841 ccgatgatta cttcatctgg ataaccggcg aaggtaaagt cgttaagaat ttaagccgcc
      901 gctttgaagc ggaacagtat gacccacagc gggtacgtgc agcggcttac tggcacgcaa
      961 agtaactgat atataaaaag aaaaaggctg acgatttctc gtcagccttt gctatatctg
     1021 gtggcccctg ctggacttga accagcgacc aagcgattat gagtcgcctg ctctaaccac
     1081 tgagctaagg ggccatggta gcggattata aagtaactcc gtgtcgcaat ccagccatta
     1141 ccgtgcgcct gctgttttta taaacaatgt attttcaatc cgttatactt ttcctggtga
     1201 tgttagaaag gagtaaatat ggttgaggat attttggctc cagggttacg ggtcgtgttt
     1261 tgcggtatca accctgggct ttcatccgcc gggactggtt ttccctttgc tcatccagca
     1321 aatcgcttct ggaaggtgat atatcaggcc gggtttaccg accgtcagtt gaagccgcag
     1381 gaggcacagc atctgctgga ttatcgttgt ggcgtcacca aactggtaga ccgtccaacg
     1441 gtgcaagcca atgaagtttc aaagcaggag ctgcacgcag gtgggcgtaa gctgattgaa
     1501 aaaattgaag attatcagcc gcaggcgttg gcgattctgg gcaaacaagc atatgaacag
     1561 ggattcagcc agcgcggtgc acagtggggg aaacaaacgc tcaccattgg ttcgacgcag
     1621 atttgggtgc tgccaaatcc cagcggttta agtcgcgttt cactagagaa actggttgaa
     1681 gcgtatcgcg agctggacca ggcgctggta gtgcgtgggc gataaaaaac gccaccgaaa
//
LOCUS       DAZPLM010000363         1644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPLM010000363 DAZPLM010000000
VERSION     DAZPLM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.366"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            52..1305
                     /locus_tag="ACHVC7_004362"
     CDS             52..1305
                     /locus_tag="ACHVC7_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2118213.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1354..1473)
                     /locus_tag="ACHVC7_004363"
     CDS             complement(1354..1473)
                     /locus_tag="ACHVC7_004363"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR2118214.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          337 a          421 c          402 g          484 t
ORIGIN      
        1 cctaacctat gtataagcct gatccacagg catatttagc aaggatttca agtgagccgt
       61 actacaactg ttgatggcgc tccggcaagc gacactgaca agcaaagcat ttctcagcca
      121 aatcaattta ttaaacgcgg tacgccgcaa tttatgcgcg tcaccctggc gctgttctct
      181 gccggactgg caacctttgc actcctctat tgtgtgcagc ctatccttcc ggtgctttcg
      241 caggagtttg gcttaacccc cgcgaacagt agtatttcac tgtccatttc cacggcgatg
      301 ttggctattg gtttgctgtt tactggcccc ctatccgatg ccattggtcg caaaccagtg
      361 atggtcacgg cgctactgtt ggcctccatt tgtacgttac tttcgacaat gatgaccagc
      421 tggcacggca ttttgattat gcgcgccttg attgggcttt cgttaagtgg cgtggcagct
      481 gttggcatga cttatcttag cgaggaaatc caccccagtt tcgtggcctt ttcgatgggg
      541 ttgtatatca gcggcaactc aattggcggc atgagcggcc gcttaattag tggtgtcttc
      601 acggactttt tcaactggcg aattgctctg gcggcaatcg gttgtttcgc gctagcctcg
      661 gcgttgatgt tctggaaaat ccttccggaa tcacgccatt ttcgcccgac ttcgctgcgc
      721 cctaagacgt tatttatcaa ctttcgtctg cactggcgtg accggggatt accgttattg
      781 ttcgcagaag gctttttgct gatggggtcg ttcgtcacgc tgtttaatta catcggctat
      841 cggttgatgc tttccccctg gcatgtcagt caggccgtgg ttggcttatt atcgctggct
      901 tatttgaccg gtacatggag ctcacccaaa gccggaacca tgaccacccg ctacgggcgc
      961 ggtccggtga tgttgttttc gacgggggtt atgctgtttg gtttactgat gaccttattc
     1021 agctcgctgt ggctgatctt tgccggaatg ttactcttct cagcaggatt cttcgcagcc
     1081 cactcagttg ccagcagctg gattggcccc cgcgcaaaac gcgctaaagg ccaggcctcc
     1141 tcgctgtatc tgttcagtta ctatctgggg tcgagtattg ccgggacgct gggtggtgtt
     1201 ttctggcata actatggctg gaacggcgtc ggcgcattta ttgctctgat gctggtcatt
     1261 gctctgctgg tcgggacgcg tttgcatcgt cgtctgcacg cctgaaaaat aagcccggac
     1321 tgcggtaact acacgtccgg acttattgct tgctcaaacc aacgttaata gccatcctaa
     1381 aatagacgaa gcgccagcca attcccgcag cgcgtctagc gtcatcagga ttataagtac
     1441 ccaaataaac ggattcattt tgctgtgtgt catttattac tgatgcgcag ttattctacc
     1501 gctttgtaag tagtaaaata gttaacccga tcaagactac tattattggt agctaaattt
     1561 cccttaagtc acaatacgtt attatcaatg ctgtaattta ttcagcgttt gtacatatcg
     1621 ttacacgctg aaaccaacca ctca
//
LOCUS       DAZPLM010000364         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPLM010000364 DAZPLM010000000
VERSION     DAZPLM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.367"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            374..721
                     /locus_tag="ACHVC7_004364"
     CDS             374..721
                     /locus_tag="ACHVC7_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR2118215.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(741..1331)
                     /locus_tag="ACHVC7_004365"
     CDS             complement(741..1331)
                     /locus_tag="ACHVC7_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118216.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1328..1588)
                     /locus_tag="ACHVC7_004366"
     CDS             complement(1328..1588)
                     /locus_tag="ACHVC7_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118217.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          356 a          462 c          409 g          403 t
ORIGIN      
        1 gcaagcaatc ccatttttcc aggtgtatca gcaaaaaaac cgctgttcta gaaatcaggc
       61 cgggtaaatt tcagagctgt atcttcaata taccattttg caaccggata caaaaccatt
      121 ccgcagagag aaatacacca gaacagtaat ctgtatttaa aatcataatc ccatgacata
      181 tacagcatat atcctcccgt cacccatccc caccacatat tattaaaata atattttctg
      241 ttcatctgcc agttttcctt tcttttgcac ttttgtcagt gtactgatgc atgacaacat
      301 aaccacagca tgataataat aatcaataac aataagctgt gtcacgttta catcatggaa
      361 cggaggtgct cctgtgactt ttgagcagaa aaaagcccgg gccattgccc tgatggacag
      421 caaaaagatg tggcggagca attatgcccc tcctcttctg cgtattctct ggcggctggg
      481 tatccggctt ccgccattac cctttatgcc gttctggcag gtcacggtgc tgacgggggg
      541 cttatggggc atttcctggg gatgtgcaat gtggtttatc tactgggggc cgtcaggaat
      601 ggtggcaggt gaggccatta ttataagtat cacggggggt tttttgtccg gactgctcat
      661 ggcctctttc cactggtggc gcaggaaagt taaccgactg ccaccatggg atgatgtgta
      721 aaccgtgcac aggcgtgttc ttacggagcg gcactttcat ttatctgtac gcgaacctcc
      781 attcctgcat cgtgacaggc ctgcagccac tgcgccactt ccagcggatc gccctcccgg
      841 cgtaccaccc agccttcttt attccataac tgcagacagg tgctgccgtc gagacgcacc
      901 acaaaatccc cacggcaggc ctgatacgtt atgccgttca gctccaccgc cagatcagtg
      961 gcagaagtgc cgcctgcggg cagacctgct gccgggacca gcggcagtac gacgacttct
     1021 gccgcacgct gttctgcccg gatgcggtcc tgttctgcca gcagcagagg ctgtgccacg
     1081 gcccggccgg cgtccgtcag ctccactgcc agctgcaggt tcggagcgcg cagggtacgc
     1141 agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc gtagaccggg
     1201 gcttccccgc tttgctccag cacccgttct atgtcccgcg tcagcacggg tcctggccgt
     1261 ttttcatcaa gggcagccag cacaatcagc actctccgct gcagtggcga cggtcggcgg
     1321 ctctgagtca tttccagtca tcctccggcc agatgagctt tctgaggcgc tgatggtcgg
     1381 cgtcaatgat ctcctgtctc tttatggcat tcattgaggt atagccattt atggccagtt
     1441 catgccagaa gatcagcacg gctccggctt tggctgactc gcagagataa gttacgccgt
     1501 ggctgtccag attgcattcc cggatgcgtt tttccgcaaa ccgggtcagt tgttcatagc
     1561 tgagtgaaaa cgtcatttcc tgatccatcc tgcacgttcc atctgataat gttctgacag
     1621 gtaaaaatca
//
LOCUS       DAZPLM010000365         1629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPLM010000365 DAZPLM010000000
VERSION     DAZPLM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.368"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHVC7_004367"
     CDS             <1..93
                     /locus_tag="ACHVC7_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2118218.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            104..292
                     /gene="dicB"
                     /locus_tag="ACHVC7_004368"
     CDS             104..292
                     /gene="dicB"
                     /locus_tag="ACHVC7_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR2118219.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="ACHVC7_004369"
     CDS             289..480
                     /locus_tag="ACHVC7_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR2118220.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="ACHVC7_004370"
     CDS             574..>1629
                     /locus_tag="ACHVC7_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR2118221.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAZPLM010000366         1625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPLM010000366 DAZPLM010000000
VERSION     DAZPLM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.369"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..1436
                     /locus_tag="ACHVC7_004371"
                     /pseudo
     CDS             1..1436
                     /locus_tag="ACHVC7_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          460 a          419 c          394 g          352 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcgaactga ggtgtactgg caatagcgga
     1561 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     1621 gggga
//
LOCUS       DAZPLM010000367         1586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPLM010000367 DAZPLM010000000
VERSION     DAZPLM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.370"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            186..1229
                     /locus_tag="ACHVC7_004372"
     CDS             186..1229
                     /locus_tag="ACHVC7_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2118222.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          452 a          354 c          337 g          443 t
ORIGIN      
        1 catttcaatc atctgacatc attataaaaa atgagattca cataaagata aaattattgt
       61 tctcaaggaa agcatgaagg ggcttcgtaa gcaatattca ttcggtgcca agacaggact
      121 tgatacacag aatctcaact tttatgctct cagactatat caccacatgt tttacaggct
      181 cagaaatgaa cgatagatta tgctttgaag tgcacgataa caagggttat ttcgtatttt
      241 cggatacctg gtttggccca ttgctggggg agtttgaaga agtgcttgat gcatacgacg
      301 ctgatgaaat cagcgagacg agttacatca acaaactccg acgccttgct caacgggagc
      361 cggactttat tgatatacac gctcatctgg catatgcatt tctggaacag aatgccccgc
      421 gtaaggcact gaacgcctct cttaaaggac tggcggcagg taatcgtatc atcccggaaa
      481 gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg gccttatctg cgcgcgcttt
      541 atgcagcaat tctcgcaaat gtgcacttac agcgccatca ggatgcggtt atgctcactg
      601 ataaaatact ggcatacaat cctgaagata atcagggggc tcgctggttg ttgggttcag
      661 agctgttacg caccggcgat catgagcgag ccttcagtgt actgaaagag catgctgatg
      721 aattttcacc ttactggtat gaactcggtc tcctgcattt tctgaacggg gaacatgtta
      781 aggccgccac agcttttcgc catggctttg ccacaaacac ttacatcgca gaaatgctgt
      841 gcggaaatct gcatccattt ccacttgctg tgtggcatga cttctccggc tctctggata
      901 cggcagaaga ttactatgca acatactcac cattatgggg acaatatccg gaagcattgc
      961 tgtttgtaaa ctggctctat aaccattcct ccgtgctgca tgaacgatca gaaatcatta
     1021 aatgcgccga aatgctgatt caggaagatg atttcgaaat atgcgaaagt attttaagac
     1081 agcaggagca cctgtggaaa agaattgaca agacactttc tgaggaaatt gtacagaaat
     1141 gcagaaatat gaacggtgaa tatatctggc cctggatatt gcccttttcc gcagcaggga
     1201 tgaaacattc aagcatccag tatcagtaga tattgcatta gtgtaacttt aaaacaagta
     1261 ataatttatt atccaataat aacactgtgt tccttcccca ggtgattacc ctggggttat
     1321 tttctgaaag tttcctttgc aaaaggatag cgttgtaaat tactttaacg aattactcca
     1381 tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa cacgttatag ctggcatcaa
     1441 aaaccacaga tttaccctgc cagcgggtaa tcccctcctc tggcgttttc ggttgaactg
     1501 gtatccccgt ggatttgccc ctatatttcc agacatctgt tatcacttaa cccattacaa
     1561 gcccgctgcc gcagatattc ccgtgg
//
LOCUS       DAZPLM010000368         1570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPLM010000368 DAZPLM010000000
VERSION     DAZPLM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.371"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            211..>1570
                     /gene="istA"
                     /locus_tag="ACHVC7_004373"
     CDS             211..>1570
                     /gene="istA"
                     /locus_tag="ACHVC7_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR2118223.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PER"
BASE COUNT          398 a          438 c          393 g          341 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc
//
LOCUS       DAZPLM010000369         1569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPLM010000369 DAZPLM010000000
VERSION     DAZPLM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.372"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..922)
                     /locus_tag="ACHVC7_004374"
     CDS             complement(<1..922)
                     /locus_tag="ACHVC7_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR2118224.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLHLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQY"
     gene            complement(948..>1569)
                     /gene="rfaY"
                     /locus_tag="ACHVC7_004375"
     CDS             complement(948..>1569)
                     /gene="rfaY"
                     /locus_tag="ACHVC7_004375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR2118225.1"
                     /translation="FDEILAYNFKTVKVLRNINDTKVSLIDTKYGRYVFKVFAPKTKR
                     NERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVE
                     GVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCT
                     AERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          491 a          327 c          269 g          482 t
ORIGIN      
        1 gatactgctt ttcattcgtg gctggaataa acgccacatc attccagcaa gaagcctgat
       61 aagcctcatt aaaatacttc tggcagggat aattcgtcca gtcgttccag ggcttcgtta
      121 cgccaacata atgaatcagc ttagtatcat cggtaatggt ctttttataa tcttcatcgc
      181 ttttattttt taactcataa tcgatgccat aaaagcaatc ataatcgccg ctaataaaaa
      241 tacaatgacc aaagaacaga atatttaaaa tatcctgatc aaggcattta tacttcgtgt
      301 gctgcgggtc acttgccagc atagcaatgg ctttattcat aaaatcattt ttcgcccatg
      361 cttcaagatg cagatatact acaccggcgt tgaaataatt ccctggaagt ccctctatag
      421 ccagacgctt cgctggtttt tcctgcataa aaggcacatc tttaataaca gctgcaaact
      481 tatccccatc aaaattaata tcaagcaatc cagctaatga gcctttacag atgacatcag
      541 catcgatata taacagattt tcaatagttt cactcagata ctggaacgca actaaacgga
      601 agaatgttgc ataagaccac tgtttcaggc tggggaagat atcaataaag gcactggaga
      661 ctttataaat tcttacttca acgttagcat ttttgctggt aaaagcatta acacgttgta
      721 gataatcttc atcaatgtag tcggtaaaga gatgaaactt aaccgcaagg tccggattat
      781 gcataacaac tgattgcata gatactgccg caccaaataa aaagttacgg tcaataccgt
      841 aagcgatatt taacacacga tcggtgttag caatttcccc agagacagcg acttcagtaa
      901 tactctcagc taataaatcc atgttggttc cgttttgact gtgtggttta cgctttgcct
      961 tttaattttt taataaattt gcgtagtttg gttctgtaga ttactgaata gtagccataa
     1021 tcttttattt cgttggcaat acccaggtga cgttccatgg ccagacgatc acgagcttta
     1081 cgctctgccg tacagctttt accggatagg tcaattatcc tgacaccatc tttactaacg
     1141 ataaagttac cccggtgtgg gtcgcccgat agcatattta aggcatgcaa tttttccatt
     1201 gaagccttaa tttctgcttt aacatcttct ggaatgatcg gcatatcatt gagctctacg
     1261 ccctcgacat actcgataag catgataaaa acactggcat agttgaaaat tttacgctca
     1321 gccagaaaat aaaaatcatt aggaaaagta agacctgcac ttctgacacg atcggtttca
     1381 acgatcaaat tctgatagta gtcacctttt acaaaagatt ttaaaaaacg ttcattcctt
     1441 ttcgttttcg gggcaaagac tttaaaaaca tagcgaccat atttcgtatc aatcaatgat
     1501 actttggtat cattaatatt acgtaatact tttactgttt taaaattata ggccagtatt
     1561 tcatcaaag
//
LOCUS       DAZPLM010000370         1541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPLM010000370 DAZPLM010000000
VERSION     DAZPLM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.373"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            109..1541
                     /gene="rhsC"
                     /locus_tag="ACHVC7_004376"
                     /pseudo
     CDS             109..1541
                     /gene="rhsC"
                     /locus_tag="ACHVC7_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          380 a          444 c          469 g          248 t
ORIGIN      
        1 gcagcgaaaa aacgagttaa aaagcggtag aaaaagttaa gttcccggtt gatcgaatgg
       61 atgcgaatcg ctgatcgaac ttcgatgcca atccgtgatc gaatagagcg gaatacgcag
      121 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
      181 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      241 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      301 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      361 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      421 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      481 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      541 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      601 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      661 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      721 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      781 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      841 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      901 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      961 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
     1021 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
     1081 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1141 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1201 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1261 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1321 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1381 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1441 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1501 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZPLM010000371         1527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPLM010000371 DAZPLM010000000
VERSION     DAZPLM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.374"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            99..362
                     /locus_tag="ACHVC7_004377"
                     /pseudo
     CDS             99..362
                     /locus_tag="ACHVC7_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(399..956)
                     /locus_tag="ACHVC7_004378"
     CDS             complement(399..956)
                     /locus_tag="ACHVC7_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR2118226.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(953..1228)
                     /locus_tag="ACHVC7_004379"
     CDS             complement(953..1228)
                     /locus_tag="ACHVC7_004379"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR2118227.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1414..1527
                     /locus_tag="ACHVC7_004380"
                     /pseudo
     CDS             1414..1527
                     /locus_tag="ACHVC7_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          326 c          368 g          409 t
ORIGIN      
        1 gccgcagaca cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg
       61 ttgttatttt tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg
      121 tggtgatgtc agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag
      181 atcaccaggt aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg
      241 atgaggttga tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga
      301 ataacctgaa tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat
      361 aaaattaacc tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc
      421 accaattgtt ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt
      481 tccagagtgc ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt
      541 ttatcgaagt ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca
      601 ggaggacgtt tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt
      661 agtctggctg cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg
      721 atcaggtagc atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg
      781 gccgaaaagt tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa
      841 acgtgtttat gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga
      901 ccatctttga aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt
      961 gatgactcca attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac
     1021 gtacagggtt ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac
     1081 gccccaccat aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat
     1141 gcgttgtgat gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa
     1201 tatagccccg gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta
     1261 taaaattaaa gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa
     1321 atcaggcagt taaaaacacg gaaaaacggt ataaaaaagc gcctcaaaat cctgtttttt
     1381 ctttcagata caatgtctta ttgcattttt taaaacatgt cagtgtggta catggatatc
     1441 gataccatcg ccaattataa actgggcggt gcacagcaac atgatagcgt acgcctcgat
     1501 ccgtgggtgt ttatgttctc agcagga
//
LOCUS       DAZPLM010000372         1514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPLM010000372 DAZPLM010000000
VERSION     DAZPLM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.375"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(162..815)
                     /gene="ribB"
                     /locus_tag="ACHVC7_004381"
     CDS             complement(162..815)
                     /gene="ribB"
                     /locus_tag="ACHVC7_004381"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR2118228.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(919..1067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1189..1479
                     /gene="ubiK"
                     /locus_tag="ACHVC7_004382"
     CDS             1189..1479
                     /gene="ubiK"
                     /locus_tag="ACHVC7_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR2118229.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          429 a          387 c          347 g          351 t
ORIGIN      
        1 ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga
       61 aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca
      121 acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca
      181 tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg
      241 gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac
      301 agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc ttcagtatga
      361 ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga
      421 ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca
      481 gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg
      541 ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct
      601 tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca
      661 acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca
      721 agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa
      781 ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat
      841 ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg
      901 gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa
      961 ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga
     1021 gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa
     1081 cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct
     1141 tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat gattgacccg
     1201 aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag
     1261 ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc
     1321 gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag
     1381 aaactggcgc ggcttgaaca aagaatgagc gaactggaaa atcgcagtac tgagattaaa
     1441 aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg
     1501 taaattatga atta
//
LOCUS       DAZPLM010000373         1502 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPLM010000373 DAZPLM010000000
VERSION     DAZPLM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.376"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..349)
                     /locus_tag="ACHVC7_004383"
     CDS             complement(<1..349)
                     /locus_tag="ACHVC7_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR2118230.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQ"
     gene            complement(330..>1502)
                     /locus_tag="ACHVC7_004384"
     CDS             complement(330..>1502)
                     /locus_tag="ACHVC7_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2118231.1"
                     /translation="MHAFNGELFVQATWDTSSSRLFRTQFRMVSPKRISNPNNTGDSR
                     NCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQT
                     RGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSKEQ
                     RERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRY
                     IAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEA
                     IVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEK
                     ECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          295 a          487 c          402 g          318 t
ORIGIN      
        1 cctgttgcag acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg
       61 cccgcgtccg gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcattacct
      121 gataactgcg ggcctgtcgt ggtccgtcat catcaccgga taacgccagc gccgcgggtg
      181 cctctccggc agtcaggctg tcgccggata ctgcatccgt caggcagctg atcccaagct
      241 ggcctgcaag cgcacaaaag aaaacccgcg cataggcggg ttcaagcatc agcggctcat
      301 taaaagccat gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc
      361 tctgttgatt gtcgcagccc ggattcaaat gccgcagccg cccaggcggg cggtttaaga
      421 ccagctgcgc ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg
      481 tcaccgcgtt tcgcgcactc tttctcgtag gtgctcagtc cggcttctat cagcatcacc
      541 gcttcctgaa cttctttcag accatcgatg gccatacgac cggagcctat ccagtcgcag
      601 ttcccccagg cactgcgggc ttcctgaaag ctgaagcgcg cttttgaagg taacgtcacc
      661 acgcggcgaa cgatggcctc ttccagccag cacagaaaca tctggctcgc ctgacgggat
      721 gcgacgaatt ttcgccgccc cataaagtac gcccacgact cgttcgcact ggcccgtgcc
      781 gtggagtagc tcatctgggc gtaattccgg gaaagttgct catacgagac acccagcccg
      841 gcagcgatat accgcaacag tgactgctca aacacggagt agccgttatc cgtgtcctga
      901 gccgtctgca ggttcagtga gtcccccggc atcaggtgcg gcacttttgc gcctcccaga
      961 cggaccggtg ctgcggcgta atacgcggca atttcaccaa tccagcccgt cagcctttcc
     1021 cgctgctcct tactgttcgc gcccagaata aaatccatcg ctgactgcgt atccagctca
     1081 ctttcaatgg tggcggcata catcgccttc acaatggcgc tctgcagctg cgtgttctgc
     1141 agcgtgtcga gcatcttcat ctgctccatt acgctgtaaa acacatttgc accgcgggtc
     1201 tgcccgtcct ccacgggttc aaaaacgtga atgaacgagg cgcgcccgcc gggtaactca
     1261 cggggtatcc atgtccattt ctgcggcatc cagccaggat agccgtcctc gctgacgtaa
     1321 tatcccagcg ccgcaccgct gtcattaatc tgcacaccgg cacggcagtt ccggctgtcg
     1381 ccggtattgt tcgggttgct gatgcgcttc gggctgacca tccggaactg tgtccggaaa
     1441 agccgcgacg aactggtatc ccaggtggcc tgaacgaaca gttcaccgtt aaaggcgtgc
     1501 at
//
LOCUS       DAZPLM010000374         1498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPLM010000374 DAZPLM010000000
VERSION     DAZPLM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.377"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            39..482
                     /gene="cmtB"
                     /locus_tag="ACHVC7_004385"
     CDS             39..482
                     /gene="cmtB"
                     /locus_tag="ACHVC7_004385"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR2118232.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            510..>1498
                     /gene="cmtA"
                     /locus_tag="ACHVC7_004386"
     CDS             510..>1498
                     /gene="cmtA"
                     /locus_tag="ACHVC7_004386"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR2118233.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          347 a          333 c          385 g          433 t
ORIGIN      
        1 cgccggacgg taccctaaaa ataaaataag gataatttat gcggcttagt gactattttc
       61 cagaatcatc aatctcagtt atacattcag caaaagattg gcaggaagct atcgatttct
      121 cgatggtatc attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag
      181 attccaccat taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg
      241 cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt gacattactt gaacaaggtg
      301 tttattttcc ggggaatgac gaaccaataa aattactcat cggcctttct gctgccgatg
      361 ccgattcgca cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac
      421 tcgaacaact cttaacagca gcatcagaaa aacaattagc ggacataatc agccgcggat
      481 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      541 aggcttttgg gggctttttg acggcaatgg tcatccccaa tattggtgct tttattgcct
      601 ggggttttat tactgcgtta tttattccca ccggttggct gcctaacgaa catttcgcca
      661 aaattgtcgg cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc
      721 atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg
      781 tcggcgcaga gatcccgatg ttccttggct caatgattat ggggccgctc ggtgggctgg
      841 tcataaaata tgttgataag gcactggaaa aacgcatacc tgccggtttt gagatggtta
      901 tcaataactt ctcattaggt atcgcgggga tgctcctttg tctgctgggt tttgaagtta
      961 tcggcccggc ggtgttaatt gccaatactc tcgtcaaaga gtgtattgag gcgctggtac
     1021 atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctca
     1081 ataatgcgat cgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg
     1141 gtaaatccat cttctttatg gtggcgtcca atcctgggcc tggcttaggg ctgctgctgg
     1201 cgtttacctt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1261 acttcctcgg tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca
     1321 ttattgccat gattgcgggc ggtatgtctg gcacctgggt gtttaactta ctggacggtg
     1381 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1441 gctcgttcct ggcgacaatt gccggtgtta cggtagctac cctggtgtcc tttgctat
//
LOCUS       DAZPLM010000375         1483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPLM010000375 DAZPLM010000000
VERSION     DAZPLM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.378"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHVC7_004387"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHVC7_004387"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR2118234.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHVC7_004388"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHVC7_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          415 a          324 c          370 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcgaatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tca
//
LOCUS       DAZPLM010000376         1471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPLM010000376 DAZPLM010000000
VERSION     DAZPLM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.379"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHVC7_004389"
     CDS             <1..122
                     /locus_tag="ACHVC7_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118235.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHVC7_004390"
     CDS             119..376
                     /locus_tag="ACHVC7_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118236.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHVC7_004391"
     CDS             376..750
                     /locus_tag="ACHVC7_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2118237.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1471
                     /locus_tag="ACHVC7_004392"
     CDS             747..>1471
                     /locus_tag="ACHVC7_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2118238.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT          406 a          312 c          424 g          329 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat g
//
LOCUS       DAZPLM010000377         1466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPLM010000377 DAZPLM010000000
VERSION     DAZPLM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.380"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            205..>1466
                     /gene="ipaHb"
                     /locus_tag="ACHVC7_004393"
     CDS             205..>1466
                     /gene="ipaHb"
                     /locus_tag="ACHVC7_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR2118239.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
BASE COUNT          445 a          353 c          252 g          416 t
ORIGIN      
        1 gtcttctggt ataaatcctc tgcattgtga atatgtaaaa catcaaaaaa agatgaacca
       61 aatatacgaa aaaagcaaat atacttacca gggtagaaag catgatataa cactcattcc
      121 cttatggcga atattctgaa tcaccctcct cattaggaga gatacattaa atacaattta
      181 ttcacaatct aattccatat ccatatgcga gaaattaata tgctcagaaa tatttcatcc
      241 tgtttatttc cacatatcag cacaattaca tcccccaacc attatttgtc cgaatgggat
      301 gattgggaga aacaggggtt accggaagaa cagcgtactg aggcggtaag aagacttcgt
      361 gcatgtctta cctctaaggg gcataaactg gacctgcgag ccttggcgct ttcctcgtta
      421 cctgtactcc ctgcttgcat taaaaagctt gatgtgagct gtaataaatt aaccatcctt
      481 actgatttac ctgaaaatat taaagaactt attgcaagag ataatttctt aacacatata
      541 tctgcattac cacattatct aataactttg gatgtgtccg aaaatcaatt agagaatctg
      601 ccgttattac cagacaccat caaatcacta agcgcagagt ataataggtt atccacactg
      661 ccttcattac ccttgaattt aaaaaaactt gaggttagga acaacgaact gcaaactctt
      721 ccatctctgc cttctaatct taagatactt aaggttgcgc acaaccatct tactgaactg
      781 ccccctttac ctaggagact gcaacttctt tttgcatata gcaatagatt aagcaactta
      841 ccaaacatcc aagaaaatat tatcatgaga agattttttt attttgaaaa caaccaaata
      901 actacaatcc cgacaaatct ttttcgttta gatcctcata taactattga gattgcaaat
      961 aaccccttat cagatcaaac tctgctattc ttaatacagc aaacttcggt tccaaatttt
     1021 aacgggcctc agtttcgtat ttccctgtca gaccaaaaca gactgttttt acgccagatg
     1081 ttgccgcaaa atttacattc gcgccatatc agagtcatca ctgaaggggg gcagaacttt
     1141 cagatccccc ctcttcccga aactgtggca gcctggtttc ctgaagcaga tcgtcgggag
     1201 gtttctacac aatggacttc tttttccacc gaggagaatt cccgggcatt ctccgcgttc
     1261 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
     1321 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcactgtt
     1381 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
     1441 aaaaccctcc tggtccatca ggcatc
//
LOCUS       DAZPLM010000378         1452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPLM010000378 DAZPLM010000000
VERSION     DAZPLM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.381"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..426)
                     /locus_tag="ACHVC7_004394"
     CDS             complement(<1..426)
                     /locus_tag="ACHVC7_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR2118240.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEK"
     gene            546..1355
                     /gene="proC"
                     /locus_tag="ACHVC7_004395"
     CDS             546..1355
                     /gene="proC"
                     /locus_tag="ACHVC7_004395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR2118241.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1372..>1452)
                     /locus_tag="ACHVC7_004396"
     CDS             complement(1372..>1452)
                     /locus_tag="ACHVC7_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118242.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT          329 a          405 c          389 g          329 t
ORIGIN      
        1 cttctccagc tccgcggcaa aggcctggcg gtcacgttgt gaaaggctat ctggtccgcc
       61 ggtctggatc ccactggcac gtaaggtatc cataaaatcg cgcatcgtca ggcgctcacg
      121 aatggtcgct ggcgtgtaac gttcgccgcg cggattaagc gccgctgcgc ctttctcgat
      181 ggcttcagca gccaaaggta tatctgcggt gatcaccaaa tcgcccgctt cacactgccg
      241 gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg cgcagcgtac gaataaatcg
      301 cgatggcggc acgcgtaaac tctggtttgc taccagtacc agcggcatct gcatacgttc
      361 cgccgcgcga tacaaaatct ctttaattac attgggacac gcgtcggcat ccacccaaat
      421 tgtcataaag tcatcctttg ttgggtaatc ctctattgtg tcgcgctttt gccttccggc
      481 atagttctgt ttatgcttct gccagcgatt atcaaaacaa tgaatttcac ggcaggagtg
      541 aggcaatgga aaagaaaatc ggttttattg gctgcggcaa tatgggaaaa gccattctcg
      601 gcggtctgat tgccagcggt caggtgcttc cagggcaaat ctgggtatac accccctccc
      661 cggataaagt cgccgccctg catgaccagt tcggcatcaa cgccgcagaa tcggcgcaag
      721 aagtggcgca aatcgccgac atcatttttg ccgccgttaa acctggcatc atgattaaag
      781 tgcttagcga aatcacctcc agcctgaata aagactctct ggtcgtttct attgctgcag
      841 gtgtcacgct cgaccagctt gcccgcgcgc tgggccatga ccggaaaatt atccgcgcca
      901 tgccgaacac tcccgcgctg gttaatgccg ggatgacctc cgtaacgcca aacgcgctgg
      961 taacccccga agataccgct gatgtgctga atattttccg ctgctttggc gaagcggaag
     1021 taattgctga gccgatgatc cacccggtgg tcggtgtgag cggttcttcg ccagcctacg
     1081 tatttatgtt tatcgaagcg atggccgacg ccgccgtgct gggcgggatg ccacgcgccc
     1141 aggcgtataa atttgccgct caggcggtaa tgggttccgc aaaaatggtg ctggaaacgg
     1201 gagaacatcc gggggcactg aaagatatgg tctgctcacc gggaggcacc accattgaag
     1261 cggtacgcgt actggaagag aaaggcttcc gtgccgcagt gatcgaagcg atgacgaagt
     1321 gtatggaaaa atcagaaaaa ctcagcaaat cctgatgact ttcgccggac gtcaggccgc
     1381 cacttcggtg cggttacgtc cggctttctt tgctttgtaa agcgccaaat ctgccgattt
     1441 caaccactca cg
//
LOCUS       DAZPLM010000379         1433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPLM010000379 DAZPLM010000000
VERSION     DAZPLM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.382"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHVC7_004397"
     CDS             <1..1117
                     /locus_tag="ACHVC7_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2118243.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..1334
                     /locus_tag="ACHVC7_004398"
     CDS             1095..1334
                     /locus_tag="ACHVC7_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118244.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGAPIKLSSML"
     gene            complement(1329..>1433)
                     /locus_tag="ACHVC7_004399"
     CDS             complement(1329..>1433)
                     /locus_tag="ACHVC7_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118245.1"
                     /translation="GKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          384 a          299 c          438 g          312 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga gggtgcccct attaagttgt
     1321 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     1381 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tcc
//
LOCUS       DAZPLM010000380         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPLM010000380 DAZPLM010000000
VERSION     DAZPLM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.383"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHVC7_004400"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHVC7_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR2118246.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHVC7_004401"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHVC7_004401"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR2118247.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHVC7_004402"
     CDS             complement(822..>1426)
                     /locus_tag="ACHVC7_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR2118248.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZPLM010000381         1425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPLM010000381 DAZPLM010000000
VERSION     DAZPLM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.384"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>1425)
                     /locus_tag="ACHVC7_004403"
     CDS             complement(<1..>1425)
                     /locus_tag="ACHVC7_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR2118249.1"
                     /translation="KMEWYMNGHIGELDKLVPHLITEHKIYGRDRITPRTVSAEDGVI
                     KKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDD
                     GYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGY
                     PKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQ
                     DLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGA
                     PIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGM
                     QHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAF
                     VENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDLSVKCLIEPESSVRFA
                     LSFRYPGWAKMMIINGVTFTKNDTQ"
BASE COUNT          431 a          290 c          262 g          442 t
ORIGIN      
        1 ttgagtatca tttttagtaa aagtaacacc attaataatc atcatttttg cccagccggg
       61 gtaacggaaa ctgagagcaa aacgaacaga tgactcaggc tcaatgagac attttacgct
      121 taaatcatga gggtattcag ttaattgtgt aattttaatt gcaacattat taaatgtgcc
      181 ataaaactca cacgggccat aaaataatgc agtaaaacca ttgttatttt cttgaaacat
      241 attttcgaca aaagccggta tggttcttgc tgtattcggt acgcagcaga cagcagcctc
      301 ctcatgagta ggggagtatt tatatctggt attaaactga ttactgtcag gatgtttcct
      361 ttcatttgct tcataacaat tatcagtctt acagtacatt atcgcgtgaa gtatgggatg
      421 ctgcatgcca aaactagcat tatagaatag ccactcagca tcatcggcca gaccaatatt
      481 agtggtcagt tttattaaca taacataagt gctaaatagt tcatgaacag aacaatattc
      541 atagcctgta taagtggcat tagctgtgcg tcctgtaatc cactcatcac caatgggagc
      601 gccagatggt gtaagataat atttcaactg ggataaaaaa atatctaatg cagtttgata
      661 ctgcggccgt tggctcgctg cgatggctaa tgccctaata tgctcatacg tatgtacgcc
      721 atgaccttta agaaggtaac cagggtctat gacattttta tattgcagat cttgctccga
      781 cacgcatcct gcggaataat cttcatacag ccacaagcaa tatgagagat attcaatgtt
      841 attggttatt tcataaaggc ggtagaaaac atctgttatt gttaatccgt ggctatgtcc
      901 agcaaaaccc tgttccacaa cgaaaggatg tgacgtttct ttagggtagc cagccataat
      961 gcattgtgcg gcttgggtaa gagaagttaa taaactctca ttacgggtgg actcataaca
     1021 tccaagcaca aaacgcaagg cagtagcttg tgcccagaac tctccgtttt ctgctttaca
     1081 gttaaatttt aatgatgaat cataaattcc aatgtagcca tcatcatctt gcgtatcgat
     1141 aagttccaaa cataaatcgc tcgctttttc ctgccattct ttattatcta acaagtaggc
     1201 actgcggcaa tatccatcct tccaattcga ttgagtttca ctatcccacc aataatattg
     1261 catcatgtta tcatcgggat tattctgttt tttaataacg ccatcttcag cagatacagt
     1321 gcgtggagtt atacgatctc ttccatatat tttatgctcc gtaataagat ggggaactaa
     1381 tttatccagt tctcctatat gaccgttcat ataccattcc atctt
//
LOCUS       DAZPLM010000382         1409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPLM010000382 DAZPLM010000000
VERSION     DAZPLM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.385"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(306..>1409)
                     /locus_tag="ACHVC7_004404"
     CDS             complement(306..>1409)
                     /locus_tag="ACHVC7_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR2118250.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          366 a          382 c          324 g          337 t
ORIGIN      
        1 ttttaaatgt gactgtcatc cttcagataa taaagccatc atcgcaatag atggaaaagc
       61 actcctgctc ttttttgatt agagccgtcg caggaggaac aactcatatc atcattgcga
      121 tctcgacgat gtatagtctc atcctgcggc agaacaagac ggataaaaaa tccaatgaga
      181 gcacactcat ccagaactta ttaacataat gaatattaaa gaaaaatcat cgcagccaat
      241 gaagtaatat gcaaaaataa ttagcccttg cagggagcaa agttccataa tctccccctt
      301 ccccctcaat gatccaaata caaataatgc atccaactgg tcattcttag cagcaccttc
      361 cgcatcaccg aaacatggct gaaatggtcg gagtaaactg ctacctgggc gtaaatgccg
      421 tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag cacgccgtcc
      481 gtgccgggaa cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc
      541 acgacaggta aaatactagt caatttgccg tggaacacct gcccaggtag cgcgttaaac
      601 accacttccg catcatcgcc aggtttcaga cgtaacagcg agttttgccg aaattgggcg
      661 acaatttgcc gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct
      721 gcgtatgtac ctgggcggat cagtacctga gtaacgtagc cattgctcgg cgcgcgaatg
      781 acagtctgct caaggttata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc
      841 gattgctcgc cgttaaccat actgtcgagc tggctctgga tctgcgcctg ctccgccacc
      901 gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt
      961 tccgagaacg gattcaccgc cgcctggctg cctttcaggt aacgttgata atttttaaac
     1021 agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca
     1081 cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc
     1141 gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta
     1201 ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg gatcgctatc
     1261 actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca
     1321 ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg tccactgatt caccggaata
     1381 cgaaagattt taaagaccgc ccacgccag
//
LOCUS       DAZPLM010000383         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPLM010000383 DAZPLM010000000
VERSION     DAZPLM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.386"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHVC7_004405"
     CDS             <1..459
                     /locus_tag="ACHVC7_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2118251.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHVC7_004406"
     CDS             477..1115
                     /locus_tag="ACHVC7_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2118252.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZPLM010000384         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPLM010000384 DAZPLM010000000
VERSION     DAZPLM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.387"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHVC7_004407"
     CDS             complement(<1..1339)
                     /locus_tag="ACHVC7_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR2118253.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPLM010000385         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPLM010000385 DAZPLM010000000
VERSION     DAZPLM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.388"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHVC7_004408"
     CDS             <1..984
                     /locus_tag="ACHVC7_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2118254.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHVC7_004409"
     CDS             999..>1377
                     /locus_tag="ACHVC7_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR2118255.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZPLM010000386         1366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPLM010000386 DAZPLM010000000
VERSION     DAZPLM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.389"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>1366
                     /locus_tag="ACHVC7_004410"
     CDS             <1..>1366
                     /locus_tag="ACHVC7_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR2118256.1"
                     /translation="ATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAA
                     SSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETA
                     AKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQ
                     DATTAQKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTK
                     NIGACRAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGC
                     GNIHLAGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNS
                     RNKPTASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYG
                     NIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAE
                     FYPVG"
BASE COUNT          387 a          352 c          386 g          241 t
ORIGIN      
        1 aagccactga gcaggccacg gcagcagcga ggtcagcttc cgcagcgaag acatccgaaa
       61 cgaacgcgaa agcgtcggaa acccgtgcag aatcctcaaa aacggctgcc gcatcgtccg
      121 ccagttcggc ggcgtcatcg gcatcatcgg cgtctgcttc aaaagatgag gcgaccagac
      181 aggcgtcagc agcgaagggc agcgccacga cggcatccac gaaggcgaca gaggctgctg
      241 gtagtgcgac ggcagcagct cagagcaaaa gtacggcgga atctgcagca acgcgcgctg
      301 agacagcggc aaaacgggca gaggatattg catccgccgt ggcgcttgag gatgcgagca
      361 cgacgaaaaa ggggatagta cagctcagta gtgcgactaa cagcacttcc gagtcactgg
      421 cggcaacacc aaaagccgtt aaggccgcgt atgacctggc taacgggaaa tacaccgcac
      481 aggatgcaac gacagcacag aaagggataa tccagctaag cagcgcgacc aacagcacgt
      541 ctgaaacgct ggcggcaacg ccaaaggcag taaaagcagc caatgacaat gctgagaaac
      601 gtctgcagaa agatcagaac ggtgcggata tccctggcaa agacaccttt acgaaaaata
      661 ttggtgcctg ccgtgccttc ggtgggtcag taagcacaac aacaggaaac tggacgactg
      721 cacagtttat cgagtggctg gattctcagg gagcatttaa ccatccatac tggatgtgca
      781 agggttcctg gtcttatggc aataataaaa tcattactga tactggctgc ggtaatattc
      841 atctcgccgg agctgtcatt gaagtaatgg ggataaagtc agcgatgacg atccgcatta
      901 ccactccgac cacatccagc ggtggcggaa caaccaacgc ccagtttacc tatattaatc
      961 acggaacaga ttattcacct ggctggcgaa gggactataa ctccagaaat aagccaacgg
     1021 catcagagat cggggcgcta ccgtcaggtg gaacagcagt atcatcagtt aatctggctt
     1081 caaaaggtcg ggtagccgcg ctgacagaca atacgcaggg ggcagcaggt cttgagttat
     1141 acgaggtgta taacaacgga tatccaacag cgtatggaaa tatcattcac ctgaaaggga
     1201 tgacagccgt tggcgaaggt gagttactca tcggctggag tggtacaagc ggtgctcatg
     1261 ctccggcatt tattcgttca cgacgggata cgaccgacgc aaactggtcg ccgtgggcgc
     1321 agctttacac ctcggctcat cctcctgcag agttttatcc agtcgg
//
LOCUS       DAZPLM010000387         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPLM010000387 DAZPLM010000000
VERSION     DAZPLM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.390"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..1261)
                     /locus_tag="ACHVC7_004411"
     CDS             complement(<1..1261)
                     /locus_tag="ACHVC7_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR2118257.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          305 g          332 t
ORIGIN      
        1 aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
       61 ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt ttcatgacct
      121 gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc tggatctcct
      181 gatcgttacc gccaacagta cgccagttgg taattgcacc actgaagatg tcacgcacct
      241 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
      301 gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc agcgcgtttt ttctgcccac
      361 cggaaggttg cgccacaaaa ataatatcag catcaccttt aacaatcctg ttatacgcat
      421 cgggggtacg agagctctcc agatattccc tggtgtgaaa atcttccggt ataacgctta
      481 aagcataaaa tgcagaggca taaattgggt acgccgccgt agcaccatcg agtcgcggcc
      541 agttttgcgt aaactgaatt tgcggtttac cacgcagtgg tgtcagttga ttattgagtt
      601 tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac accgacaccg ggattgagct
      661 gtttatataa ataaccctga taaccagcga caaaaccaag catgagaagg ataaacagga
      721 gggaattacg gcaacgtagc ccggatgtac cggttaaagg atgtctggtt ggccggccgt
      781 accccagcga aaaactgata tgcgcgccaa cgggtataat gactatccac cagtactcac
      841 ctccgactac tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac
      901 ccgatcccat ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca
      961 ggattaataa accgggtact aaaaaaagac attgtcgcca gacggctatt tctctttttt
     1021 ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa tagcattaat gcgcccagcg
     1081 ccagcataaa aatgttgagc agaaggtaac ttgttggcgt gataaggata ttacaaactt
     1141 caataatacc ggcaagtccg acacccagca tagcaacaac cgccgccaaa aattgcgtca
     1201 gtatggggat gccgaaaaaa gtcattacca gcgaggacaa aatccatttt ctgttttgca
     1261 ttattctttc cattcttttt gaatggtgaa attatactcc ccgagtcccc ttgccccttc
     1321 tggacacttt tccgaaatga tggcggaaaa aaacgggacc ctttg
//
LOCUS       DAZPLM010000388         1354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPLM010000388 DAZPLM010000000
VERSION     DAZPLM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.391"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..936
                     /locus_tag="ACHVC7_004412"
     CDS             <1..936
                     /locus_tag="ACHVC7_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR2118258.1"
                     /translation="SYPFTTEKVYLSQIPGLVNMALYVSPIVSGEVIRSRGGSTSEFT
                     PGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRRRRIIMQNMRDEELAIAQVEEMQAV
                     SAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSGVTEWSKRDKSTYDPTDDIEAYALN
                     ASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGSHSELETAVKDLGKAVSYKGMYGDV
                     AIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYPK
                     NWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
     gene            978..>1354
                     /locus_tag="ACHVC7_004413"
     CDS             978..>1354
                     /locus_tag="ACHVC7_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR2118259.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTER"
BASE COUNT          308 a          356 c          410 g          280 t
ORIGIN      
        1 agctatccct tcactacgga gaaagtctat ctctcacaaa ttccgggact ggtaaacatg
       61 gcgctgtacg tttcgccgat tgtttccggt gaggttatcc gttcccgtgg cggctccacc
      121 tctgaattta cgccgggata tgtcaaaccc aagcatgagg tgaatccgca gatgaccctg
      181 cgtcgcctgc cggatgaaga tccgcagaat ctggcggacc cggcttaccg ccgccgtcgc
      241 atcatcatgc agaacatgcg tgacgaagag ctggccattg ctcaggtcga agagatgcag
      301 gcagtttctg ccgtgctcaa gggcaaatac accatgaccg gtgaagcctt cgatccggtt
      361 gaggtggata tgggccgcag tgcggcgaac aacatcacac agtccggcgt cacggagtgg
      421 agcaagcgtg acaagtccac gtatgacccg accgacgata tcgaagccta cgcgctgaac
      481 gccagcggtg tggtgaatat catcgtgttt gacccgaaag gctgggcgct gttccgttcc
      541 ttcaaagccg tcaaggagaa gctggatacc cgtcgcggct ctcattccga gctggagaca
      601 gcggtaaaag acctgggcaa agcggtgtcc tataagggaa tgtatggcga tgtggccatc
      661 gtcgtgtatt ccggacagta cgtggaaaac ggcgtcaaaa agaacttcct gccggacaac
      721 acgatggtgc tggggaacac tcaggctcgc ggtctgcgca cctatggctg cattcaggat
      781 gcggacgcac agcgcgaagg cattaacgcc tctgcccgtt acccgaaaaa ctgggtgacc
      841 accggcgatc cggcgcgtga gttcaccatg attcagtcag caccgctgat gctgctggct
      901 gatcctgatg agttcgtgtc cgttcaactg gcgtaatcat ggcccttcgg ggccattttc
      961 tctctgtgga ggagttcatg acgaaagatg aactgattgc ccgtcttcag gagctgggtg
     1021 agcaattgaa ccgcgatgtc agtttgacgg gaacgaaaga agaactggcg cttcgtgtgg
     1081 cagagctgga agaagagctt gatgacacgg atgaaactgc cggtcaggac acccctctca
     1141 gccgggaaaa tgtgctgacc gggcatgaaa atgaggtggt atcagcgcag ccggataccg
     1201 tgattcagga tacggctgaa ctggtcacgg tcgtggcact ggtgacgctg catactgatg
     1261 cacttcacgc cacgcgggat aaacctgtgg catttgtgct gccgggaacg gcgtttcgtg
     1321 tctctgccgg tgtggcagcc gaaatgacag agcg
//
LOCUS       DAZPLM010000389         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPLM010000389 DAZPLM010000000
VERSION     DAZPLM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.392"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHVC7_004414"
     CDS             <1..189
                     /locus_tag="ACHVC7_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118260.1"
                     /translation="SATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQL
                     AQGKRRCQQQFTSQEVQN"
     gene            199..>1346
                     /gene="yicI"
                     /locus_tag="ACHVC7_004415"
     CDS             199..>1346
                     /gene="yicI"
                     /locus_tag="ACHVC7_004415"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR2118261.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          336 a          320 c          353 g          337 t
ORIGIN      
        1 agcgccacga ttagcatcat tattgcgcta ttcacgattg ttccggcgat ctgttatttg
       61 ctgagcgcga ttatcgctaa acgctactac tcactcacga cgcacaatct gaaaaccgtt
      121 atggaacagc tggctcaggg taaacgccgt tgccagcaac aattcacctc tcaagaagtg
      181 cagaactaag gaacggcaat gaaaattagc gatggaaact ggttgattca acctggcctc
      241 aatttgattc acccgcttca ggtgttcgag gttgaacagc aggataatga aatggtggtc
      301 tatgctgccc cccgtgatgt gcgtgaacgt acctggcagc ttgatacgcc tttatttacg
      361 ctgcgctttt tctccccaca ggaaggtatt gtcggtgtgc ggattgagca ttttcagggg
      421 gcgctgaata acggtcctca ttatccgctc aatattttgc aggacgtgaa ggtcacaatc
      481 gaaaacacag aacgttatgc tgagtttaaa agtggcaact taagcgcgcg tgtcagcaaa
      541 ggtgagttct ggtcactgga ttttctgcgc aacggcgaac gtattaccgg tagtcaggtg
      601 aaaaataatg gctacgtgca ggacacgaat aatcaacgca attatatgtt tgagcggctt
      661 gatcttggcg ttggcgaaac agtttacggt ctgggagagc gctttactgc cctggtgcgc
      721 aatggccaga cggtagagac ctggaaccgg gacggcggca caagtactga acaggcgtat
      781 aaaaatatcc cgttctacat gactaaccgt ggttatgggg tactggtcaa tcatccccag
      841 tgtgtctctt ttgaagtggg atcggagaaa gtctccaaag tgcagttcag cgttgagagt
      901 gaatatctcg aatactttgt tatcgacggc ccgacgccga aagcggtact tgatcgttat
      961 acccgcttta ctggtcgtcc ggcgctgccg cccgcgtggt ccttcggcct gtggctaacc
     1021 acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga tggtatggcg
     1081 gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa agccttccag
     1141 tggtgcgatt ttgagtggga cccgctgact ttccctgacc cggaagggat gatccgccgc
     1201 ctgaaagcga aagggctgaa aatctgcgtc tggattaacc cctatatcgg tcaaaaatcc
     1261 cccgtcttta aagagttaca agagaaaggc tatttactca aacgcccgga cggttcgcta
     1321 tggcagtggg ataaatggca gccagg
//
LOCUS       DAZPLM010000390         1342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPLM010000390 DAZPLM010000000
VERSION     DAZPLM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.393"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..1135
                     /locus_tag="ACHVC7_004416"
     CDS             <1..1135
                     /locus_tag="ACHVC7_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR2118262.1"
                     /translation="NKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSND
                     VSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSS
                     YNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLC
                     LASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDV
                     LTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDI
                     IQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQM
                     TISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          366 a          285 c          267 g          424 t
ORIGIN      
        1 taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg gccttatcta
       61 tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac tgatccagac
      121 aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta tttgtttaac
      181 ctcgctggca ctcttttgtt atggaaaaga caacatcctt gacaacaata agaaaaaaac
      241 gggaatcctg ttgctggcgc tgattccttt tttagttttt ccccttctgg cacacaatct
      301 gagcagttat aacgctgact attctttgta tgtcgtcgac tactgtccgg acaaccatac
      361 tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat ttttactgtt
      421 ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag cattattatg
      481 tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata attacaccat
      541 ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt cttttctgat
      601 ttataacatt tttcagaaac tgcaactctc cagcaaactg gcagttcatg atgtgctgac
      661 caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt cgagacctgt
      721 tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca tcaatgccca
      781 atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta tccagcaaag
      841 catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct tgctatttac
      901 cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact
      961 cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca gtattggcac
     1021 ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag cagataaagc
     1081 cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata tttaagtgca
     1141 aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta ctattacccc
     1201 taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg tgccaggccg
     1261 caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa
     1321 attgcaccat agaatgctcc gg
//
LOCUS       DAZPLM010000391         1332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPLM010000391 DAZPLM010000000
VERSION     DAZPLM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.394"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            373..828
                     /gene="csgB"
                     /locus_tag="ACHVC7_004417"
     CDS             373..828
                     /gene="csgB"
                     /locus_tag="ACHVC7_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR2118263.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            869..1321
                     /gene="csgA"
                     /locus_tag="ACHVC7_004418"
     CDS             869..1321
                     /gene="csgA"
                     /locus_tag="ACHVC7_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR2118264.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          397 a          263 c          318 g          354 t
ORIGIN      
        1 gttttttctc cacacctccg tggacaaatt tttactccaa aaagacgagg tttgtcacgg
       61 cttgtgcgca agacatatcg cagcaatcag cgatgggcaa gaagaatgac tgtctggtga
      121 tttttgatag cggaaacgga gatttaaaag aaaacaaaat attttttgcg tagataacag
      181 cgtatttacg tggcttttaa tactttggta tgaacaaaaa aagaaaaata caacgcgcgg
      241 gtgagttatt aaaaatattt ccgcagacat actttccatc gtaacgcagc gttaacaaaa
      301 tacaggttgc gttaacaacc aagttgaaat gatttaattt cttaaatgta cgaccaggtc
      361 cagggtgaca acatgaaaaa caaattgtta tttatgatgt taacaatact gggtgcgcct
      421 gggattgcag ccgcagcagg ttatgattta gctaattcag aatataactt cgcggtaaat
      481 gaattgagta agtcttcatt taatcaggca gccataattg gtcaagctgg gactaataat
      541 agtactcagt tacggcaggg aggctcaaaa cttttggcgg ttgttgcgca agaaggtagt
      601 agcaaccggg caaagattga ccagacagga gattataacc ttgcatatat tgatcaggcg
      661 ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg gtaatactgc gatgattatc
      721 cagaaaggtt ctggtaataa agcaaatatt acacagtatg gtactcaaaa aacggcaatt
      781 gtagtgcaga gacagtcgca aatggctatt cgcgtgacac aacgttaatt tccattcgac
      841 ttttaaatca atccgatggg ggttttacat gaaactttta aaagtagcag caattgcagc
      901 aatcgtattc tccggtagcg ctctggcagg tgttgttcct cagtacggcg gcggtaacca
      961 tggtggtggc ggtaataata gcggcccaaa ttctgagctg aacatttacc agtacggtgg
     1021 cggtaactct gcacttgctc tgcaaactga tgcccgtaac tctgacttga ctattaccca
     1081 gcatggcggc ggtaatggtg cagatgttgg tcagggctcg gatgacagct caatcgatct
     1141 gacccaacgt ggcttcggta acagcgctac tcttgatcag tggaacggca aaaattctga
     1201 aatgacggtt aaacagttcg gtggtggcaa cggtgctgca gttgaccaga ctgcatctaa
     1261 ctcctccgtc aacgtgactc aggttggctt tggtaacaac gcgaccgctc atcagtacta
     1321 atacatcatt tg
//
LOCUS       DAZPLM010000392         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPLM010000392 DAZPLM010000000
VERSION     DAZPLM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.395"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHVC7_004419"
     CDS             complement(<1..346)
                     /locus_tag="ACHVC7_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118265.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHVC7_004420"
     CDS             complement(349..1059)
                     /locus_tag="ACHVC7_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118266.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHVC7_004421"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHVC7_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2118267.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZPLM010000393         1291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPLM010000393 DAZPLM010000000
VERSION     DAZPLM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.396"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHVC7_004422"
     CDS             <1..396
                     /locus_tag="ACHVC7_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118268.1"
                     /translation="EEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSK
                     ENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNL
                     AMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            406..906
                     /locus_tag="ACHVC7_004423"
     CDS             406..906
                     /locus_tag="ACHVC7_004423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118269.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            982..>1291
                     /locus_tag="ACHVC7_004424"
     CDS             982..>1291
                     /locus_tag="ACHVC7_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118270.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
BASE COUNT          464 a          175 c          294 g          358 t
ORIGIN      
        1 gaggaaatgt tatccggtga atatgctggt ttcgtttata tctttcagtt tgaggatggt
       61 tctaattatg tcggtgctaa gcaaatctat cagcgggtta aggatgcgag aaagctaaag
      121 cccacttcaa aggaaaatgg ctggcgtgag tacctcagta gttccaatgt cgttaaaaac
      181 aaaattgaaa atggtgaaca gtactcaaaa acgattctat ggtgcttcgc aactatgcga
      241 gaggtaatga ttgtagaggc aatactgatc cttcatcaga tgcttaaacc ggaatgtttg
      301 aatctggcaa tgatgagtaa gattagatca ccaaatgcaa aggataaaaa aagactactc
      361 ggaatagtac aggaactatt ggagtacttg aattgaggta aatatatggc aagtggaata
      421 aatgggattg gtagtataag gaactatata caaacgcagg gagttagatt aaataatcaa
      481 ttccagaatg aaataattaa acgttccaag ctactatccc aaagaatgca gggtgatttg
      541 aataatagcg ttgaccgtgg ggcagtgggc tttactcaac gttccatcct tttcttctat
      601 agaaaggttg ggagaaattc agttacatcg accatcatgg ttaaagatat tcaggctaag
      661 tacctttatc aagtactggt acagccaaag gcaattgata agtttatacc tacttcatct
      721 acaagattaa ctaagcaggg caacatttca ggactgaaaa agaatctttt aagtgaacga
      781 tataaggtag tgaagggtaa gaatggtaaa gaacgattaa ttgataccgc aaagaaagat
      841 accaagaaga aaaccaaacg tgtaattggc ttacgtgaga agaaacaacg tcgccttatc
      901 tattgacttc tataaggaag ccaatgatgg tgtaagggta attatgagcg gtattcaggg
      961 aacattcagg ataagtagac aatgaatttt gagcaacatt atggtgattt aataagcaag
     1021 attaagctaa atggcatgac accagatggt aatagtatgc caattaacaa atgttttctt
     1081 gataagaaat ttcaaaaggt cgttaagaaa agtaattatc agattgatag ctatatgaac
     1141 gttatttatg agaagaagaa agatttcaaa gttggtgaag tacttgaatg ggaattacag
     1201 ggtgaaaaga tgcctgtatt tctaattgag agtaaaaagc tctttgtgaa gggtaaacat
     1261 ttttgggtat atgccgtggg gattattgaa t
//
LOCUS       DAZPLM010000394         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPLM010000394 DAZPLM010000000
VERSION     DAZPLM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.397"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHVC7_004425"
     CDS             132..1151
                     /locus_tag="ACHVC7_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR2118271.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAZPLM010000395         1254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPLM010000395 DAZPLM010000000
VERSION     DAZPLM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.398"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHVC7_004426"
     CDS             complement(<1..185)
                     /locus_tag="ACHVC7_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR2118272.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHVC7_004427"
     CDS             complement(230..1216)
                     /locus_tag="ACHVC7_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR2118273.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          342 a          284 c          257 g          371 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt aacc
//
LOCUS       DAZPLM010000396         1239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPLM010000396 DAZPLM010000000
VERSION     DAZPLM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.399"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..1239
                     /gene="rhsB"
                     /locus_tag="ACHVC7_004428"
                     /pseudo
     CDS             1..1239
                     /gene="rhsB"
                     /locus_tag="ACHVC7_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          300 a          360 c          383 g          196 t
ORIGIN      
        1 tgccgcgccg ctggtgcgct atggctggac gccacgcggc gaactggcgg tggtgtatga
       61 ccgtagtggc aaacaggtgc gcagctttac ttacgatgat aaataccggg gccggatggt
      121 ggcgcaccgt cacacgggcc ggccggaaat ccgttaccgt tacgacagcg acgggcgggt
      181 gacagaacag cttaacccgg caggcttaag ctacacgtat cagtatgaga aggaccgcat
      241 caccatcacc gacagcctga accgccgtga agtgctgcac acgtagggtg aagccgggct
      301 gaagcgggtg gtgaaaaagg aacacgcgga cggcagcgtc acgcagagtc agtttgacgc
      361 cgtgggcagg ctcaggacac agacggatgc cgcaggccgg acaacagaat acagcccgga
      421 tgtggtgacg ggcctcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta
      481 ctataaccac cacagccagt taacgtcagc caccgggcct gacgggctgg aaatgcgccg
      541 caaatatgat gaatccggcc gtctgattca ggaaaccgcc cctgacggcg atatcacccg
      601 ctaccgttat gataatccac acagtgactt accctgcgca acggaagatg ccaccggcag
      661 ccggaaaacc atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg
      721 ttatgtaacc cgttatgacc atgaccgttt tggtcaggtg acggcggtgc accgcgagga
      781 agggctgagc cagtaccgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac
      841 gcagggccat gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc
      901 cccggacggc agcagaaacg ggacacagta cgatgcgtgg ggaaaagcca tctgtaccac
      961 gcagggcggg ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgtctgac
     1021 cagtgaaaac ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga
     1081 aaccggcttt gacggccgca cacagcgtta tcaccacgac ctgaccggca aacttatccg
     1141 cagcgaggat gaggggctgg tcacccactg gcactatgac gaagcagacc gcctcacgca
     1201 ccgcaccgtg aagggtgaaa ccgcagagcg gtggcggta
//
LOCUS       DAZPLM010000397         1220 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPLM010000397 DAZPLM010000000
VERSION     DAZPLM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.400"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            79..375
                     /locus_tag="ACHVC7_004429"
     CDS             79..375
                     /locus_tag="ACHVC7_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR2118274.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            381..>1220
                     /locus_tag="ACHVC7_004430"
     CDS             381..>1220
                     /locus_tag="ACHVC7_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2118275.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          366 a          278 c          300 g          276 t
ORIGIN      
        1 gcatagccta tgcgcctgca atccctgaca tcgcaatgat gattgccaac aagcttgaaa
       61 attttggagt gaccagtgat gagcgcttac ccgatttttg atcgtattga agaaatggca
      121 tgggctcgcc actaccagca gatcgttcgt gaagaaaaag aaacggaact ggcggacgac
      181 ctggaaaaag gtctgcctca gcacctgttt gaatcgctct gcatcgacca tttgcaacgc
      241 cacggtgcca ataaacaggc aatcagccac gcatttgatg atgatgttga gtttcaggag
      301 cgcatggcag aacacatccg gtacatggct gaaaccattg cccaccacca ggttgatatt
      361 gatttggagg actaaaatct atggcgcttt ttcaacgagc aataaacaca caggcctatc
      421 taaaagcagg aattatgggg tttgctggcg acggcaaaac ctacacagca agcgaactgg
      481 ctattggtct ggtaatgctt atgcggcaac ggggaattga agctggtaac cgcccggtaa
      541 tgtttctgga cacagaaaca ggctctgact gggttaaacc ccgctttgat gcggaaaata
      601 tcgaattatt tacggcaaaa acccgctcgt ttgttgatct gcttgaagcc attaatgaag
      661 cggaaagcag cggttccgta atgatcatcg actccatcag tcacttctgg accggattat
      721 gcgacgagta tgccagacgc cgaaaccgta aacgaggact ggaattttca gactgggcgt
      781 ggctcaaaca ggagtggcga cgctttaccg atcgatttgt taacagtcag gcgcacatta
      841 ttatgtgcgg gcgtgcagga tatgagtatg actttttcga aggtgatgat ggcaaacgcc
      901 agttggcaaa aaccggtata aaaatgaaag cagaaacaga aaccggttat gaaccctcca
      961 tcctgatcca gatggagaag caaatgaaca tagaaaccgg tcaggtgtgg cgcaccgcca
     1021 gaatactaaa agatcgctcc acccgtattg atggacaggt attttcaaac ccaacattta
     1081 aaaactttct tcctcatatc gagtttctta acctgggagg tgaacacctc ggcatcgata
     1141 cctcacggga taacagtgaa ttatttgcca acgacggtac accgacatgg ctaaaagaaa
     1201 agcgtgcaaa agaaatcgca
//
LOCUS       DAZPLM010000398         1216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPLM010000398 DAZPLM010000000
VERSION     DAZPLM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.401"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            185..703
                     /locus_tag="ACHVC7_004431"
     CDS             185..703
                     /locus_tag="ACHVC7_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR2118276.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            913..>1216
                     /locus_tag="ACHVC7_004432"
     CDS             913..>1216
                     /locus_tag="ACHVC7_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR2118277.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          296 a          307 c          315 g          298 t
ORIGIN      
        1 gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct
       61 cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc
      121 ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt
      181 acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc
      241 aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat
      301 gctggtgcag gaattcctgc ataacgttac tgtcccgacc actcctcagt ccggtcagcc
      361 tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca aagcggttcc
      421 gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac ttcactggtg
      481 gcgtacctct gtggagggca aacaggagca ttatttcact acccgcctga ctgattcgac
      541 gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac gcgagttcac
      601 ccagttgctc gaggtttcac tggcctaccg caaaattgaa tgggaacacg tcaaatctgg
      661 cacctccggt gccgacgact ggcgcgcacc actggaagca taagttaagc caacagcatc
      721 cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag
      781 gggttacgtt cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt gcctgaacgc
      841 aagcgtaaca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt ttccttaacc
      901 ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg cctgccaccg
      961 gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct cttttccctc
     1021 gagctctctc tggtcagcca gcagtttctc tcccttgaat ttccgcaggt actggacaaa
     1081 atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg
     1141 acctggtttg aactggggga gaacgacaaa aaccagatgc tgtacagcat gaaggtatgc
     1201 ccaccgctgt ggcgca
//
LOCUS       DAZPLM010000399         1215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPLM010000399 DAZPLM010000000
VERSION     DAZPLM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.402"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            349..582
                     /locus_tag="ACHVC7_004433"
     CDS             349..582
                     /locus_tag="ACHVC7_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR2118278.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            640..1050
                     /locus_tag="ACHVC7_004434"
     CDS             640..1050
                     /locus_tag="ACHVC7_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR2118279.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          413 a          271 c          226 g          305 t
ORIGIN      
        1 aggccaaaaa gcccgttttc agcacctgtc gtttcctttc ttttcagggg gtgttttaaa
       61 taaaaacatt aggttacggc gaagaagaac ggaaacgcct taaaccggaa aattgtcata
      121 aatagcgaaa acccgcgcgc cttccgcccc gtaacctgtc ggatcgccgg aaaggacccg
      181 caaaatgata ataattatca tctacatgtc acaacgtgca tctacgccat caaaccacgt
      241 caaataatca attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa
      301 acaacttcag acaatacaaa tcagcgacac tgaatacagg gcaacctcat gtcaacgaag
      361 aacagaaccc gcagaacaac aacccgcaac atccgctttc ctaaccaaat gattgaacaa
      421 attaacatcg ctcttgttca aaaagggtcc gggaatttct cagcctgggt cattgaagcc
      481 tgccgccgga gactgtgctc agaaaaaaga gtttcgcctg aagcaaacaa agaaaagagt
      541 gacattactg aattgctcag aaaacaggtt agaccagatt gaagcaattt agataatcgt
      601 gcagactacg ccccctcata tcacatggaa ggtactacaa tggctcaggt tgccattttt
      661 aaacaaatat tcgataaagt gcgaaataat ttaaactatc actggtttta ttctgaacta
      721 aaacgtcaca atgtctcaca ttacatttac tatttagcca cagagaatat tcatcttgtt
      781 cttgaaaacg ataatacggt tttaataaaa ggacagggta aggttgtaaa tgtaagattt
      841 tcaaaaaata aatgccttat agaagccacc ttaaaaggat tcaaatcagg agagttatca
      901 ttttacgaat acaggaaaaa tcttgctaca gcaggggttt tcagatggat tacaaatatc
      961 cacgaaaaca aaaggtatta ctataccttt gataattcat tactctttac tgagaacata
     1021 cagaacacta cacaaatatt tccgcactaa atcataacgt ccggtttctt ccgtgccaga
     1081 accggactcg ctggcatgat gaaatatgtg tacccggtaa ccccggtgtg catcgttttt
     1141 gattattccc gcacactcgc gcagaaggag ttccccgtcg ggctacggtc tctgttaata
     1201 cgggaatacg gcgac
//
LOCUS       DAZPLM010000400         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPLM010000400 DAZPLM010000000
VERSION     DAZPLM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.403"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..543
                     /locus_tag="ACHVC7_004435"
     CDS             <1..543
                     /locus_tag="ACHVC7_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118280.1"
                     /translation="KPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKS
                     TNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGS
                     LTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNA
                     AGNAENTVKNIAFNYIVRLA"
     gene            543..1059
                     /locus_tag="ACHVC7_004436"
                     /pseudo
     CDS             543..1059
                     /locus_tag="ACHVC7_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          347 a          268 c          309 g          274 t
ORIGIN      
        1 aagcccgcca gtggtcgtgc cgtattgtct caggaacagg atggcattaa atcgcacacc
       61 cacagcgcca gcgcatccag tacggatttg gggacgaaaa ccacatcgtc gtttgattac
      121 ggcactaaat ccacgaataa caccggggcg catacgcaca gtctgagcgg ctctacaagt
      181 tcagctgggg cccaccaaca tagtcagact gggcccagaa cgaatagcgg gagtcaacct
      241 actggaatgt ttccggcagg gagtactcaa gttagcggga ctaatcaggt tggtatcagt
      301 ggctcattaa ccagtggcac ctcccaatgg gtaggtaaat catcttcaga aggaaaccat
      361 acacactcac tgtccggcac agcagcatct gcgggtgcac acgcgcatac tgtcggtatt
      421 ggtgctcata cgcactctgt tgcgattggt tcacatggac acaccatcac cgttaacgct
      481 gctggtaacg cggaaaacac cgtcaaaaac atcgcattta actatattgt gaggctcgca
      541 taatggcatt cagaatgagt gaacaagcac ggaccataaa aatttataac ctgctggccg
      601 gaactaatga atttattggt gaaggtgacg catatattcc gcctcataca ggtctgcctg
      661 caaacatcgt ggcatctcgt tgaagatcat cggggtaaaa cggtttatga cgtggcttcc
      721 ggcgacgcgt tatttatttc tgaactcggc tcattaccgg aaaatgtcat ctggttatcc
      781 ccggaagggg agtttcagaa gtggaacggc acagcctggg tgaaggatac ggaagcagaa
      841 aaactgttcc ggatccggga ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt
      901 gagcatatag cgccgcttca ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc
      961 ttgttgctgg aagcctggaa aaagtatcgg gtgttgctga accgtgttga tacatcaact
     1021 gcaccggata ttgaatggcc agtagcacct atagggtaaa ttcgtaatga ttacctaaat
     1081 acgttattct tttgttaaaa aagtgatttt gctataggta gggagtatgt tcttggatta
     1141 agaataaaaa atcgccatga aagagcttca tggcgagtac ataaacgtac aaaatgtg
//
LOCUS       DAZPLM010000401         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPLM010000401 DAZPLM010000000
VERSION     DAZPLM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.404"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..786
                     /locus_tag="ACHVC7_004437"
     CDS             <1..786
                     /locus_tag="ACHVC7_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2118281.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            799..>1198
                     /locus_tag="ACHVC7_004438"
     CDS             799..>1198
                     /locus_tag="ACHVC7_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR2118282.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSN"
BASE COUNT          390 a          238 c          229 g          341 t
ORIGIN      
        1 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
      781 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
      841 attgtgcaaa gtgctccctg tgcattgacc aatgtcgggg aacaacgtgg aacatatata
      901 ctcaagccac tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa caccggaaat
      961 atcattgagt gtgcagactt acaagagacg accaacgcca ttaaagttca ttttttaaca
     1021 cagaacgctt taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa
     1081 tctgacggta aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaaca
//
LOCUS       DAZPLM010000402         1194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPLM010000402 DAZPLM010000000
VERSION     DAZPLM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.405"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            45..830
                     /locus_tag="ACHVC7_004439"
     CDS             45..830
                     /locus_tag="ACHVC7_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118283.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(967..>1194)
                     /gene="ykfM"
                     /locus_tag="ACHVC7_004440"
     CDS             complement(967..>1194)
                     /gene="ykfM"
                     /locus_tag="ACHVC7_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR2118284.1"
                     /translation="VVNFATDITIQLTLIFLLIAIVIAPLIAPFVTGYVNANYHPCGN
                     NTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
BASE COUNT          391 a          211 c          257 g          335 t
ORIGIN      
        1 gctaaacatt accatgatta tgacaggcga gggagtgtcc gattatgaat ttaagaaggg
       61 tttgttgtat atgtgtatta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagctgc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa gggcatcaga aagcgattat tgttttccag gatacgttgc
      541 agacaggaaa atatgactct ggattgaaat caatagtttg gtcagattat aaaaatcaaa
      601 aattgactga cacgatgtca ctgcgctatc tggtacgatt cacgctggtg gatgtagcta
      661 caggtgagtg ggctgcctgg tcgccggtga attatgaata taaagtgctt ccaccattgc
      721 ccgacaagaa tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc
      781 aaaagaccta taaagcgatg gtaaaagatt tggttaaccg ctatcaataa taaattatat
      841 ctgccgccag gaatttctgg cggaaataat acaaaattct ttgcataaga aatttttctt
      901 gacaaagaca gtgcaagaaa ggacatggaa tccggagtta aaaactgttt gttttagaat
      961 gtttaattat ttaactgctg aatcttcctt gcgagaaata tattcattat tacatttcat
     1021 gccattttta atatagattg ctcctgggaa aattcccgta ttattaccgc aggggtgata
     1081 attagcattg acatatcctg tgacaaaagg agctattaaa ggtgctatta cgatagctat
     1141 tagtaaaaat ataagagtta gctgtattgt tatgtctgtg gcgaaattga ctac
//
LOCUS       DAZPLM010000403         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPLM010000403 DAZPLM010000000
VERSION     DAZPLM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.406"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHVC7_004441"
     CDS             complement(<1..755)
                     /locus_tag="ACHVC7_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR2118285.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHVC7_004442"
     CDS             1071..>1187
                     /locus_tag="ACHVC7_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118286.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPLM010000404         1179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPLM010000404 DAZPLM010000000
VERSION     DAZPLM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.407"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..118
                     /gene="rcbA"
                     /locus_tag="ACHVC7_004443"
     CDS             <1..118
                     /gene="rcbA"
                     /locus_tag="ACHVC7_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR2118287.1"
                     /translation="KLTKSECEKMLSGEKEAGVSREQKVKLINFNCEKLQSS"
     gene            218..433
                     /gene="xisR"
                     /locus_tag="ACHVC7_004444"
     CDS             218..433
                     /gene="xisR"
                     /locus_tag="ACHVC7_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR2118288.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            435..>1179
                     /locus_tag="ACHVC7_004445"
     CDS             435..>1179
                     /locus_tag="ACHVC7_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR2118289.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          408 a          213 c          271 g          287 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgtttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 tgcattgtat tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa
      181 tttcagccta acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga
      241 agagtggatg gttgaatacg gcctgatgct tcgcactggt ctgggtgcca gacaaattga
      301 agcataccgc cagaactgtt gggtggaagg cttccacttc aaacgagtat ctcctttagg
      361 gaagccagac agtaagcgag ggattatctg gtacaactat ccgaagataa atcagtttat
      421 caaagactca tgatatgtct aaattaccaa caggtgtcga gattcgaggt aaatacattc
      481 gcatctggtt catgtttcga ggaaaacgat gtcgggaaac attgaaaggc tgggagatta
      541 ctaacagtaa cattaaaaag gccgggaatt taagagcgtt gatagttcat gaaatcagtt
      601 ccggtgaatt tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga
      661 caacgagggt cataaaaaca ttcggggagc tttgtgatat ctggacaaaa attaaagaga
      721 cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcaggataa
      781 taatttgtga gagtaccccg atatcgcata ttcgttatag cgatatctta aactaccgga
      841 atgaactgct gcatggagaa acgctttacc tagataatcc aagatccaac aaaaaaggaa
      901 gaaccgtgcg cacagttgat aactatatcg ccctgctctg ttcgttgtta cgttttgcgt
      961 atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca
     1021 gaataaagcc tgacccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag
     1081 gacagagcca aaacttgtgg aaatttgccg tttactccgg gcttcgtcac ggggaactgg
     1141 cagctctggc gtgggaggat gtggatttcg agaagggaa
//
LOCUS       DAZPLM010000405         1175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPLM010000405 DAZPLM010000000
VERSION     DAZPLM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.408"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(95..599)
                     /locus_tag="ACHVC7_004446"
                     /pseudo
     CDS             complement(95..599)
                     /locus_tag="ACHVC7_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
     gene            complement(616..>1175)
                     /locus_tag="ACHVC7_004447"
     CDS             complement(616..>1175)
                     /locus_tag="ACHVC7_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2118290.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          355 a          224 c          184 g          412 t
ORIGIN      
        1 tcttcacttt taattatcgt attcattata aaactaagtt aaaaaaactc ctagagagtg
       61 agttattaat atatttttaa ataaaattaa gttatcattc atcgatgcat tttatagaat
      121 catcaatggc cataatagta ctattaactc tcaattttat atttttatca tcactacaaa
      181 tattaactga atttatatca atgatttttt tattcttatc ttttatttac tgtttcaatg
      241 caatcagcgg caagttggag tggatttaga tttttcagat atggtcttat gatctttccc
      301 ttacttaata aatagcatgt ataggattcg gaaagtccag catctatttt gtacctcatt
      361 atattagata aggatgtgag aaattccttg ttatcattcg cagccatata tgccagtccg
      421 agttcagcct ctgcggcctt acaggcaaaa tagcttagtg aacacgcttt tcgtccctct
      481 ttagtactgg ctgatattag tgcccataat attgctttgt ctgccaaatc taactctctc
      541 tgaaattgtc ttgcagtaac tgtactcgca gaaatagaga aaaaaataca attattaata
      601 actttgccat attttttagt ccgtaactgt taatgtgcta tctggctctg aaaatattaa
      661 ctcatttaat ttttgcatgt cattagatga accagttata caaccctcgg aacagaatcg
      721 aggtcctaaa gacggaccga aagcgtttaa acaactttgt gttaaaaatc catcacgatt
      781 atatgtttct gtatttgctg acggtactaa acgtatacca ttaggttttc tattggttgc
      841 tccaggacca ttaacattcc agctccatac cccttgaggt attggacctc tattttgtaa
      901 gtgtgtacaa ccaggattat ttttacattg catattatta ccattattac cagaggcgac
      961 aggaatagta agcacatcat aagagttatc atcattttgc gtatctgaaa cgcatgaaag
     1021 catacttaaa gttatgctgt ataaacaggt cgctaatccc aacgggtcaa tatcagttat
     1081 cggattcaac ggatactgat aaaaattcca tccccccttc agtccaatct tttcgcgccg
     1141 gcgtgtacac cgggtccatc tggttttgca tctgc
//
LOCUS       DAZPLM010000406         1170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPLM010000406 DAZPLM010000000
VERSION     DAZPLM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.409"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHVC7_004448"
     CDS             <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHVC7_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR2118291.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          295 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg
//
LOCUS       DAZPLM010000407         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPLM010000407 DAZPLM010000000
VERSION     DAZPLM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.410"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            118..225
                     /gene="yniD"
                     /locus_tag="ACHVC7_004449"
     CDS             118..225
                     /gene="yniD"
                     /locus_tag="ACHVC7_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR2118292.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(278..1036)
                     /locus_tag="ACHVC7_004450"
     CDS             complement(278..1036)
                     /locus_tag="ACHVC7_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR2118293.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          325 a          285 c          274 g          278 t
ORIGIN      
        1 gaaacagtga ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat
       61 gcctatccct tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg
      121 ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt
      181 ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa
      241 gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata
      301 aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag ttccatgtga
      361 cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg
      421 agttcagcgt tgtatcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt
      481 cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat
      541 tgtcggtata tttgtcgtaa cgcacacctg ggccgaattc aaagcggaaa ctgtgtaccg
      601 ggccattcaa aaactggcga ccataacccg cggtcaacac atcgcgctct cgatagccgt
      661 tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa
      721 aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat
      781 tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt
      841 cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat
      901 taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat
      961 cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg
     1021 tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc
     1081 ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg
     1141 cgcgaataac ttagcaatgt at
//
LOCUS       DAZPLM010000408         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPLM010000408 DAZPLM010000000
VERSION     DAZPLM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.411"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHVC7_004451"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHVC7_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR2118294.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1161)
                     /locus_tag="ACHVC7_004452"
     CDS             complement(510..>1161)
                     /locus_tag="ACHVC7_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR2118295.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAZPLM010000409         1155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPLM010000409 DAZPLM010000000
VERSION     DAZPLM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.412"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHVC7_004453"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHVC7_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1155
                     /locus_tag="ACHVC7_004454"
     CDS             572..>1155
                     /locus_tag="ACHVC7_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR2118296.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          274 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggt
//
LOCUS       DAZPLM010000410         1147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPLM010000410 DAZPLM010000000
VERSION     DAZPLM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.413"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            53..769
                     /locus_tag="ACHVC7_004455"
     CDS             53..769
                     /locus_tag="ACHVC7_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2118297.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          368 a          211 c          232 g          336 t
ORIGIN      
        1 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
       61 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
      121 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
      181 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
      241 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
      301 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
      361 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
      421 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
      481 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
      541 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
      601 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
      661 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
      721 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
      781 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
      841 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
      901 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
      961 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     1021 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     1081 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     1141 tttttac
//
LOCUS       DAZPLM010000411         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPLM010000411 DAZPLM010000000
VERSION     DAZPLM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.414"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHVC7_004456"
     CDS             <1..187
                     /locus_tag="ACHVC7_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR2118298.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHVC7_004457"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHVC7_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2118299.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAZPLM010000412         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPLM010000412 DAZPLM010000000
VERSION     DAZPLM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.415"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHVC7_004458"
     CDS             <1..>1140
                     /locus_tag="ACHVC7_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR2118300.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPLM010000413         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPLM010000413 DAZPLM010000000
VERSION     DAZPLM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.416"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHVC7_004459"
     CDS             84..1115
                     /locus_tag="ACHVC7_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR2118301.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPLM010000414         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPLM010000414 DAZPLM010000000
VERSION     DAZPLM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.417"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHVC7_004460"
     CDS             complement(<1..643)
                     /locus_tag="ACHVC7_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR2118302.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKFFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHVC7_004461"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHVC7_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR2118303.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          323 a          252 c          196 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 agaactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAZPLM010000415         1099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPLM010000415 DAZPLM010000000
VERSION     DAZPLM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.418"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>1099)
                     /locus_tag="ACHVC7_004462"
     CDS             complement(<1..>1099)
                     /locus_tag="ACHVC7_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR2118304.1"
                     /translation="DQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELSTWDTIYL
                     HLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDNEVEIKA
                     GNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHTIGSKIA
                     NVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIALMNTFY
                     DSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQD
                     TVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASN
                     LVNLKTLLNDGFFIKYSHFNDVALIKQNISNL"
BASE COUNT          349 a          208 c          168 g          374 t
ORIGIN      
        1 agagattact tatgttttgc tttattaatg ccacgtcatt aaaatgacta tatttaatga
       61 aaaaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct tcacgtacca
      121 tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc aagtgaatca
      181 tgcattgcgt tccctttgtc atatccacac atacgctaca aatatcgtgg taatgcgaag
      241 agcgcatttc aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat cgtgttaata
      301 aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca agcactattg
      361 tatgctctac atctaacgca gtttcaaatg gctcttcttc tgtaaaatca ttatttaaaa
      421 cctcacctga cgtattatca ctgtcataaa aagtgttcat caatgcgata ttatttgtta
      481 cagcgtcttt ttcgatattc tcactaattg catatcgcct gctataatca taaaaatgaa
      541 taaacgcgcc ctttataaag gatatatatt gttctttgtt aaagtcaata cggctgtata
      601 atttttcaaa aatcacattc gcaattttac taccaatggt atgcctgtat aataatgggt
      661 gcgattcaat cgtaaaatgc ttgtctaatt gatcttgtaa tttattctca tagtccagag
      721 ggaaaagaaa ctgttgtctt tcaagtttcc cattcgatac attacgtatt ccaatagaga
      781 ttctttcatt acccgccttt atttcaactt cgttatcttc tgatttaccc agatattttt
      841 taacatcagt ctcgaaagca ttactaaaaa aagtattaaa ttggtaattc gctgatggca
      901 tattttccgc gagcaacaca tcatttttta tgagatcact gtcagcattt attgctaata
      961 tcagatgcaa gtagatggta tcccatgtag acaactcacc cttacgactg gaatttaata
     1021 tcgcgaaaaa ttccctcagt gaatccagga aattatcact ttgatttagt tgtgtgcctg
     1081 gaaatgatga tgcttgatc
//
LOCUS       DAZPLM010000416         1094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPLM010000416 DAZPLM010000000
VERSION     DAZPLM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.419"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            198..617
                     /locus_tag="ACHVC7_004463"
     CDS             198..617
                     /locus_tag="ACHVC7_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR2118305.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            617..>1094
                     /gene="umuC"
                     /locus_tag="ACHVC7_004464"
     CDS             617..>1094
                     /gene="umuC"
                     /locus_tag="ACHVC7_004464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR2118306.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          270 a          232 c          287 g          305 t
ORIGIN      
        1 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
       61 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
      121 tagcctgaat cagtattgat ctgctggcaa gaacagatta ctgtatataa aaacagtata
      181 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
      241 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg
      301 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
      361 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
      421 atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
      481 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
      541 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
      601 ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga
      661 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
      721 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg
      781 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
      841 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
      901 cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga
      961 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     1021 tggtatgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa
     1081 atggcagcgg caga
//
LOCUS       DAZPLM010000417         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPLM010000417 DAZPLM010000000
VERSION     DAZPLM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.420"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHVC7_004465"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHVC7_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHVC7_004466"
     CDS             294..>1087
                     /locus_tag="ACHVC7_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118307.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPLM010000418         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPLM010000418 DAZPLM010000000
VERSION     DAZPLM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.421"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            528..>1087
                     /gene="fimB"
                     /locus_tag="ACHVC7_004467"
     CDS             528..>1087
                     /gene="fimB"
                     /locus_tag="ACHVC7_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR2118308.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          332 t
ORIGIN      
        1 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaaccag gcaggaataa
       61 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg tgttccatga
      121 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      181 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      241 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatca
      301 ggttgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
      361 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      421 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
      481 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
      541 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      601 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      661 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      721 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      781 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      841 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      901 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      961 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     1021 ttgggcatcg caatattcgt catactgtct ggtataccac cagcaatgca gggcgttttt
     1081 acggcat
//
LOCUS       DAZPLM010000419         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPLM010000419 DAZPLM010000000
VERSION     DAZPLM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.422"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            247..>1070
                     /locus_tag="ACHVC7_004468"
     CDS             247..>1070
                     /locus_tag="ACHVC7_004468"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR2118309.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHF"
BASE COUNT          308 a          201 c          253 g          308 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaggg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa
//
LOCUS       DAZPLM010000420         1064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPLM010000420 DAZPLM010000000
VERSION     DAZPLM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.423"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>1064)
                     /locus_tag="ACHVC7_004469"
     CDS             complement(<1..>1064)
                     /locus_tag="ACHVC7_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2118310.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDEKG"
BASE COUNT          164 a          412 c          294 g          194 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgc
//
LOCUS       DAZPLM010000421         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPLM010000421 DAZPLM010000000
VERSION     DAZPLM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.424"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHVC7_004470"
     CDS             <1..120
                     /locus_tag="ACHVC7_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2118311.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHVC7_004471"
     CDS             120..410
                     /locus_tag="ACHVC7_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR2118312.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHVC7_004472"
     CDS             407..961
                     /locus_tag="ACHVC7_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR2118313.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAZPLM010000422         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPLM010000422 DAZPLM010000000
VERSION     DAZPLM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.425"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            236..>1044
                     /locus_tag="ACHVC7_004473"
     CDS             236..>1044
                     /locus_tag="ACHVC7_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2118314.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHP"
BASE COUNT          355 a          219 c          179 g          291 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa
     1021 agagataaaa ctagaacatc cgtt
//
LOCUS       DAZPLM010000423         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPLM010000423 DAZPLM010000000
VERSION     DAZPLM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.426"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            3..614
                     /locus_tag="ACHVC7_004474"
     CDS             3..614
                     /locus_tag="ACHVC7_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR2118315.1"
                     /translation="MYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWIDDGDTSLPPEK
                     IQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGKNA
                     LKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNV
                     KVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGNYISDKI"
     gene            630..>1044
                     /locus_tag="ACHVC7_004475"
     CDS             630..>1044
                     /locus_tag="ACHVC7_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR2118316.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          339 a          188 c          240 g          277 t
ORIGIN      
        1 ttatctacgg gacgcgcatt atttacccgg cagaaaataa agaagtgatg gtgcagttga
       61 tgaaccaggg aaaccgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcat
      121 taccaccaga aaaaattcag gttcctttca tgttaacgcc accagtggca aaaatagggg
      181 caaattccgg gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag
      241 aaagcatttt ttatctgaat gttctggata ttccaccaaa tagtccagag caagaaggta
      301 agaatgcact gaagtttgcg atgcaaaaca gaattaagtt gttttaccga ccagcgggta
      361 ttgctccggt aaataaagcg acatttaaaa aattgctggt aaatcgcagt ggcaatggtt
      421 tggtgataaa aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg
      481 tcaaagtcaa ttatgaaact attatgattg cccccttaga aagtcagagt gttaatgtca
      541 aaagtaataa tgcaaataac tggcatctga ccattatcga tgaccatggc aactatatta
      601 gtgacaaaat ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa
      661 tcgtagcgtt attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt
      721 ttatgatagg tggaatgaaa gaccagcagg ttgcaaatat tcgtcttgat gataatcaac
      781 ccttaccggg gcagtatgac atcgatattt atgtcaataa gcaatggcgc gggaaatatg
      841 agattattgt taaagacaac ccgcaagaaa catgtttatc aagagaagtt atcaagcggt
      901 taggcattaa tagcgataac ttcgccagcg gtaagcaatg tttaacattt gagcaacttg
      961 ttcagggtgg gagctatacc tgggatatcg gggtttttcg tctcgatttc agtgtcccgc
     1021 aggcctgggt ggaagaactg gaaa
//
LOCUS       DAZPLM010000424         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPLM010000424 DAZPLM010000000
VERSION     DAZPLM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.427"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            113..799
                     /locus_tag="ACHVC7_004476"
     CDS             113..799
                     /locus_tag="ACHVC7_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR2118317.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(890..1026)
                     /locus_tag="ACHVC7_004477"
     ncRNA           complement(890..1026)
                     /ncRNA_class="other"
                     /locus_tag="ACHVC7_004477"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT          277 a          224 c          253 g          288 t
ORIGIN      
        1 tcaaaacatg atactaacta tatattgatc gttgcattgt tggcatgtaa cacgctgcag
       61 cgagtaacat tggtaatgaa atataatcaa catggatagg tgaaaattaa ttatgagctt
      121 tatggctttt gtaacttgtt tgacaattgc atttatcgcc ggttttgctg ttgccagaaa
      181 attgacactc cgaaaagttg cctcatcggt aatcatcgct aaatcgcaac ccgtcccttc
      241 atctgcaact tcccctgccg ctaaggcgtc gacaaggagc tatataccgc agaaaaccta
      301 tcgtgactct cgttggcaag agcgagatga gttatttcag ggaatggttg ccgaaggtat
      361 tttattggaa agaaaagagt ggttaatcac gcgttgtgaa aaaatgtatt tcgataatct
      421 ggaaaaatgg tttggacatt attgtcatat ccattgccag gtctctctgg ggcgcttgat
      481 caactttcca gaacaacctg agtttagcga agaagaaaga aagcgtttct ttgcaattta
      541 taatggcatg gcaatggatt atgttctggt ttcaaagaag aacaataaaa tagtctgtgt
      601 tattgagctg gatgacccga gtcatgaaag ggcggatcgc attattcgtg accgttggtt
      661 ggatacaatt atgactcttg cacgggtgcc ttttgtacga gcttctgtca ctaaattgga
      721 ggaaattcct ccggtctggg agactcgtga caaaattcgc ccattagtcg cggcgacaaa
      781 gagtgaaccg gtgcattaac taagtatccc ggtcggggct tctcatcccc ccggtgtgtg
      841 caatatacga aaaaaagccc gtaccttcgt acgagctctt ctttaaagat ggcggtgagg
      901 gggggattga ctcgcttcgc tcgccctgcg ggcagcccgc tcactgcgtt cacggtctgt
      961 ccaactggct gtcgccagtt gtcgaacccc ggtcggggct tctcatcccc ccggtgtgtg
     1021 caatatacga aaaaaaagcc cg
//
LOCUS       DAZPLM010000425         1031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPLM010000425 DAZPLM010000000
VERSION     DAZPLM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.428"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>1031)
                     /locus_tag="ACHVC7_004478"
     CDS             complement(<1..>1031)
                     /locus_tag="ACHVC7_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR2118318.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDRQG"
BASE COUNT          172 a          384 c          284 g          191 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg c
//
LOCUS       DAZPLM010000426         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPLM010000426 DAZPLM010000000
VERSION     DAZPLM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.429"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHVC7_004479"
     CDS             complement(77..865)
                     /locus_tag="ACHVC7_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR2118319.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHVC7_004480"
     CDS             complement(905..>1023)
                     /locus_tag="ACHVC7_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR2118320.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPLM010000427         1015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPLM010000427 DAZPLM010000000
VERSION     DAZPLM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.430"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            15..251
                     /gene="rclB"
                     /locus_tag="ACHVC7_004481"
     CDS             15..251
                     /gene="rclB"
                     /locus_tag="ACHVC7_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR2118321.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            263..856
                     /gene="rclC"
                     /locus_tag="ACHVC7_004482"
     CDS             263..856
                     /gene="rclC"
                     /locus_tag="ACHVC7_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR2118322.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
BASE COUNT          288 a          204 c          219 g          304 t
ORIGIN      
        1 tgatggtatt ttatatgttt aaaaaatctg ttttatttgc aacactatta tctggcgtta
       61 tggaattttc caccaatgca gatgataaaa taattctgaa acatatcagc gtctcgtcag
      121 tatcagcatc accgacagtt ctggaggata ccattgctga tatagccaga aaatataatg
      181 cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca acagcagtat
      241 tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc ggggagataa
      301 aattggcctg tcattgattc gtctgagtat tgcgattgtt tttatgtgga ttgggttatt
      361 aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca gtccactaat
      421 gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg aaggcgaata
      481 caaaccagaa gcaagggcat ggcaatcggc caataatacc tatggttttt ccaacggtct
      541 tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca atcgctggtt
      601 aggtttattg ggaggactga tggcatatac cacgccgctg gtaacactct catttttaat
      661 caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt tcccttattt
      721 atccggtgct ggtcgcttgg tattgaaaga tactctgatg ctggcaggtg ctgtcatgat
      781 aatggcagat tcggcgcggg aaattcttaa acaacgcagt aatgaatcca gttcaacgtt
      841 aaaaactgaa tattgataat ccgaacactg tctgttgttc actttttcag gcgtcaggcc
      901 ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag tgaagatcaa
      961 tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatatatgat taata
//
LOCUS       DAZPLM010000428          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPLM010000428 DAZPLM010000000
VERSION     DAZPLM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.431"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHVC7_004483"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHVC7_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR2118323.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPLM010000429          966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPLM010000429 DAZPLM010000000
VERSION     DAZPLM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.432"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHVC7_004484"
     CDS             complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHVC7_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR2118324.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            232..903
                     /gene="queE"
                     /locus_tag="ACHVC7_004485"
     CDS             232..903
                     /gene="queE"
                     /locus_tag="ACHVC7_004485"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR2118325.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          270 a          201 c          227 g          268 t
ORIGIN      
        1 gttatcatta aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg
       61 attaaatcca ttttctttat tttcttctga cataatacgt tactcccttt aattattaac
      121 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
      181 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      241 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      301 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      361 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      421 agtgataagt ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga
      481 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca
      541 ctgactgatc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      601 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      661 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      721 cgcgtacgcg atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg
      781 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      841 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      901 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      961 tattgt
//
LOCUS       DAZPLM010000430          961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPLM010000430 DAZPLM010000000
VERSION     DAZPLM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.433"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHVC7_004486"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHVC7_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR2118326.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZPLM010000431          940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPLM010000431 DAZPLM010000000
VERSION     DAZPLM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.434"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..940
                     /gene="espL4"
                     /locus_tag="ACHVC7_004487"
                     /pseudo
     CDS             1..940
                     /gene="espL4"
                     /locus_tag="ACHVC7_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          336 a          179 c          169 g          256 t
ORIGIN      
        1 agcccagaca aatgaacacc aaaccctgag tgaattgttt tataaagaac tcgaggataa
       61 attttctggc aaggagctgg cgacgcctct attaaaaagc ttctcagaga actgtagaca
      121 aaatggtcgt catatcttta gcaacaagga ttttgtcatt aaattttcca cgtccgtctt
      181 acaagctgat aagaaagaaa ttacgataat taataaaacg aaaacacgac actcactcaa
      241 accattgctc caatatttga agaataccta atggaaattt tacctcaacg ctcagacact
      301 cttgataaac aagaattaaa cctaaaatca gatagaaaag aaaaagaatt cccaagaatt
      361 aaacttaatg gtcaatgtta ttttccgggg cgaccccaaa accgtatagt atgccgacac
      421 attgctgcac aatatattaa tgatatttat cagaatgttg attacaaacc ccatcaagat
      481 gattattctt cagctgaaaa atttctcact cacttcaaca aaaaatgcaa aaaccagact
      541 ttggcgttga tttccagccg tcctgagggg cgttgcgttg ctgcctgcgg tgatttcggg
      601 ctagttatga aagcatattt tgacaagatg gaatcaaatg gcatcagtgt tatggcagcc
      661 atattactgg tggataacca tgctttgacg gtccggctaa gaataaagaa cacaactgaa
      721 ggatgtaccc attacgtggt ttcggtttat gatcctaatg taactaacga caaaataaga
      781 atcatgagcg aaagcaaaga ggatattaaa cactattctc tgatggattt tatgaatgta
      841 gattatagcc tcctgaaatg gtcaaatgat catgttatta accaatctgt tgcaataatt
      901 ccagcacttc cgaaagaaca gctattgata ttaaaaggat
//
LOCUS       DAZPLM010000432          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPLM010000432 DAZPLM010000000
VERSION     DAZPLM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.435"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(195..>933)
                     /locus_tag="ACHVC7_004488"
     CDS             complement(195..>933)
                     /locus_tag="ACHVC7_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2118327.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAZPLM010000433          928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPLM010000433 DAZPLM010000000
VERSION     DAZPLM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.436"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..928
                     /locus_tag="ACHVC7_004489"
                     /pseudo
     CDS             1..928
                     /locus_tag="ACHVC7_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          180 a          310 c          247 g          191 t
ORIGIN      
        1 ctaatatctg acctgaaaca acatggtcag atactgctgg ttgttgatca gccagctacc
       61 atcggtgcgt tacctgtcgc cgttgcccgc tcagaaggag tccttgtcgg atacctccct
      121 ggactggcca tgcgccgcat agccgactta cacgccggtg aagctaaaac tgatgctcgt
      181 gacgctgcca tcattgccga agctgcccgt accctgcctc acgcgctacg cacgctgaaa
      241 ctggctgacg agcaaatcgc cgaactctcc atgctctgcg gcttcgatga tgatcttgcc
      301 gcacagacaa cgcaggccag caaccgtatc cgcggccttc tgacccagat acatccggca
      361 ctggagcgcg ttctcggtcc gagacttgag cacccggcgg tactcgatct tctccagcga
      421 tatccctcac cagaaaaact cgcttcgctg ggcttcgctg ggtgagaaga agctggcagc
      481 ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca tagctcaggc
      541 actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag tactgccacg
      601 tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc ttgaggtaga
      661 acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg gagtcggtgt
      721 caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct ctgccgcaca
      781 tctcgctgct tatgctggcc ttgcgccggt aactcggcga tccggctcgt caatacgcgg
      841 tgagcatccc tcgcgacggg gtaataaagc tctcaaacgg gcgttgttcc tgtcggcctt
      901 cgccgcgctc agggatccgc tctccagg
//
LOCUS       DAZPLM010000434          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPLM010000434 DAZPLM010000000
VERSION     DAZPLM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.437"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHVC7_004490"
     CDS             <1..72
                     /locus_tag="ACHVC7_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118328.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..>923
                     /gene="alaE"
                     /locus_tag="ACHVC7_004491"
     CDS             481..>923
                     /gene="alaE"
                     /locus_tag="ACHVC7_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR2118329.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQV"
BASE COUNT          244 a          205 c          203 g          271 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaa
//
LOCUS       DAZPLM010000435          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPLM010000435 DAZPLM010000000
VERSION     DAZPLM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.438"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHVC7_004492"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHVC7_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR2118330.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          267 a          146 c          229 g          279 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg a
//
LOCUS       DAZPLM010000436          908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPLM010000436 DAZPLM010000000
VERSION     DAZPLM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.439"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>908
                     /locus_tag="ACHVC7_004493"
     CDS             <1..>908
                     /locus_tag="ACHVC7_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR2118331.1"
                     /translation="NGIPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAID
                     GKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGK
                     IITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAM
                     SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRY
                     YISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAI
                     NVLTNDKVFKAGLRRKMRKAAMDRNY"
BASE COUNT          303 a          164 c          217 g          224 t
ORIGIN      
        1 aaaatggcat tcctgttcac gataccattg ccagagttgt atcctgtatc agtcctgcaa
       61 aatttcacga gtgctttatt aactggatgc gtgactgcca ttctacagat gataaagacg
      121 tcattgcaat tgatggaaaa acgctccggc actcttatga caagagtcgc cgcaggggag
      181 cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggtcatcgga cagatcaaga
      241 cggatgagaa atctaatgag attacagcta tcccagaact tcttaacatg ctggatatta
      301 aaggaaaaat catcacaact gatgcgatgg gttgccagaa agatattgca gagaagatac
      361 aaaaacaggg aggtgattat ttattcgcgg taaaaggaaa ccaggggcgg ctaaataaag
      421 cctttgagaa aaaatttccg ctgacagaat taaataatcc agcgcatgac agttccgcaa
      481 tgagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc gatgtccctg
      541 atgaacttat tgatttcacg tttgaatgga aagggctgaa aaaattatgc gtggcagtct
      601 cctttcgctc aataatagca gagcaaaaga aagagcccga aatgatggtc agatattata
      661 tcagttctgc tgatttaacc gctgagaagt ttgcgacagc gattcgaaat cactggcacg
      721 tggagaataa tctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa
      781 gaagaggaaa tgcagcagaa ttattttcag ggatacggca cattgctatt aatgttttga
      841 cgaatgataa ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggaca
      901 gaaactat
//
LOCUS       DAZPLM010000437          890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPLM010000437 DAZPLM010000000
VERSION     DAZPLM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.440"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..570)
                     /locus_tag="ACHVC7_004494"
     CDS             complement(<1..570)
                     /locus_tag="ACHVC7_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR2118332.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(570..>890)
                     /locus_tag="ACHVC7_004495"
     CDS             complement(570..>890)
                     /locus_tag="ACHVC7_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR2118333.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          211 c          194 g          188 t
ORIGIN      
        1 cgcaagaccc attaagatcc ctggcaaata tcccgccata aatagcgatg ctacagaaac
       61 gccgccagct gtcagggaaa acacaattaa cacgtttgag ggcggtatca gtaatccagt
      121 aatacaagat gagacgttta cagcggtaga aaatgccgga tcataacctt gtttggtctg
      181 aatgggattc agtgtgccac caacggcagc agctgccgca acggctgaac cagaaatgga
      241 accaaacatc atattcgcca ggacgttcac atgaccaagt gaaccaggaa cccgaccaac
      301 cattacctgg gcgaggttaa tcaaacgtat cgcaataccg ccgctgttca tcagcgttcc
      361 ggcaaagatg aaaaagggga ttgccaatag ggcaaagtta tccagtccat tagccagtcg
      421 ctgcgaaaca gtgattgccg cgatatccca gggaaacatc aaaagcattg aagctacagt
      481 ggcaataccg attgagaacg aaatgggcac accaataaag accagcacaa aaaaacagcc
      541 aaacagtgta atcgcgatat aactatccat taactggcct cctgcgcagg ttgtttcagt
      601 tgttgaatgc tttgattaat aaaacacaag gcataaaaca tcatcaccaa tccacttaat
      661 ggcagaatga tatatacgta tcccatcggt atttgcatcg cggcagatac ctgtgatgtt
      721 gccagcgttt tatcaattaa tttcaggcca ccagtcacaa tgaccgaacc agcaaaacct
      781 aaaataagaa cgttaatgac tatactaaga agtaattgct tgcgcttatt cagggcgaga
      841 gcaaataaat cgatagagag atgtctctgc gcgccgacgg tatatgcagc
//
LOCUS       DAZPLM010000438          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPLM010000438 DAZPLM010000000
VERSION     DAZPLM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.441"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>876
                     /gene="cadA"
                     /locus_tag="ACHVC7_004496"
     CDS             <1..>876
                     /gene="cadA"
                     /locus_tag="ACHVC7_004496"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR2118334.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          220 c          212 g          206 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
      721 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
      781 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta
      841 atgactccgt atgctgcgtt ccagaaagag ctgcac
//
LOCUS       DAZPLM010000439          862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPLM010000439 DAZPLM010000000
VERSION     DAZPLM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.442"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHVC7_004497"
     CDS             <1..343
                     /locus_tag="ACHVC7_004497"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR2118335.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..776)
                     /locus_tag="ACHVC7_004498"
                     /pseudo
     CDS             complement(405..776)
                     /locus_tag="ACHVC7_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
     gene            complement(794..>862)
                     /locus_tag="ACHVC7_004499"
     CDS             complement(794..>862)
                     /locus_tag="ACHVC7_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR2118336.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          234 a          207 c          192 g          229 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaaaacgg
      781 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
      841 gcagctccac cgattttgag aa
//
LOCUS       DAZPLM010000440          843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPLM010000440 DAZPLM010000000
VERSION     DAZPLM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.443"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(263..>843)
                     /locus_tag="ACHVC7_004500"
     CDS             complement(263..>843)
                     /locus_tag="ACHVC7_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2118337.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          249 a          170 c          156 g          268 t
ORIGIN      
        1 cataagcatc cctttaatta tcccctcaac ctcttgtaac tttcccactc ttgaactcca
       61 catagtccaa acgccggatg caaaaaaatc acacgtgcat ttctgaaatt gtgatggagg
      121 aagatagtat caattatatg tgcttataaa cgtccccgat tagcgcagaa gtcaccggat
      181 tgttcaggct ccaataggtt gagtatgccg tatttatttt ccaaaataaa aatcgatgct
      241 gttgctcctt cataaaatac ttttactctc tggacgcttt tcttagctct cttggtgtta
      301 tcttaaaacg attatggaac ctttcagtaa aacgagaagg acacttgtaa ccattttccc
      361 tggcaatctc gctgatgggt tttactgtcg tttgtatagc agacaacgca ttatttaacc
      421 tcacgtcgtc caatatactt tgaaaactta ctccctcgct tgccagacgg cgatgcaatg
      481 tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt gttccaggtt
      541 cggatagcat taggttataa caacgactta ttaatgattg tttactatct gataaaagta
      601 aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa
      661 tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa tatattaact
      721 cggaattctg aggtaagcag aaacctgtcg tgttacgaat ttgatcggtt atcgcataaa
      781 gattttggaa cttttcaatt agatcaatgg gataataaag catttctgca agataaagac
      841 ggt
//
LOCUS       DAZPLM010000441          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPLM010000441 DAZPLM010000000
VERSION     DAZPLM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.444"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(354..461)
                     /locus_tag="ACHVC7_004501"
     CDS             complement(354..461)
                     /locus_tag="ACHVC7_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2118338.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          169 a          252 c          195 g          218 t
ORIGIN      
        1 caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg tgatgttgtc
       61 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      121 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg attctacgac
      181 tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc cgcagaatat
      241 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cacccctgcg
      301 gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc acgttacttc
      361 cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac cggagccgct
      421 aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc tgtattgtaa
      481 tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc ccgtggtgtt
      541 gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg
      601 gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg ctgattctac
      661 gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca ttccgcagaa
      721 tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag tttcactcct
      781 gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat gaca
//
LOCUS       DAZPLM010000442          823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPLM010000442 DAZPLM010000000
VERSION     DAZPLM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.445"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            185..751
                     /gene="slp"
                     /locus_tag="ACHVC7_004502"
     CDS             185..751
                     /gene="slp"
                     /locus_tag="ACHVC7_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR2118339.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          253 a          171 c          178 g          221 t
ORIGIN      
        1 ggattatatt atggggcgat ggatactcat gccacgcccc aaaatcaatg gaattgaact
       61 aaacaaaagg gatataaaca tcagacaggt ttacgttact atcaggcata tcgcctcaga
      121 atcagatgaa aactataaag aaatatctat tatggtttta atatttgttg ataaggatag
      181 taacatgaac atgacaaaag gtgcactcat cctcagcctt tcatttttgc ttgccgcatg
      241 tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct gatattcaaa aaagttttgt
      301 tgctgttcat aaccagccgg ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt
      361 tatcaacgtt atcaatggca aaacggatac gttgttagaa atcgctgtat taccgttgga
      421 tagctatgcg aaacctgata ttgaagccaa ctatcagggc cgactgctcg ccagacaaag
      481 cggcttcctt gacccagtga actatcgtaa tcactttgtt accatcctcg gtaccattca
      541 gggtgaacaa cctggcttta tcaataaagt cccgtataac ttcctggaag tgaatatgca
      601 gggcatccag gtgtggcatt tgagagaagt ggttaatacc acctataacc tgtgggatta
      661 cggctatggt gcattctggc cggaaccggg ctggggtgcg ccttactaca ccaatgcggt
      721 gagtcaggta acacctgagc tggtcaaata acaccaccga aagatgcaga tgctttctca
      781 gcatctgcat catgtattac ataaaattaa tatacagtaa gct
//
LOCUS       DAZPLM010000443          816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPLM010000443 DAZPLM010000000
VERSION     DAZPLM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.446"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..660
                     /locus_tag="ACHVC7_004503"
     CDS             <1..660
                     /locus_tag="ACHVC7_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2118340.1"
                     /translation="GKSTIGQTLLKPRLEGSEIIKVETLNTDGTNDEKLAAKEFDEII
                     KRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVREAKQQIDSIQTITT
                     LMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGENPVVVYHNNIFNILN
                     KSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNGFNSDLDVAFKNLNL
                     V"
BASE COUNT          298 a          130 c          151 g          237 t
ORIGIN      
        1 gggaaatcga ccattggtca aactttattg aaacctcgtc tggaagggtc agaaattata
       61 aaagtagaaa cattaaacac tgatggtaca aatgacgaga aattagcagc aaaggagttt
      121 gacgaaatta tcaaacgtat tgatgatagt gaatgcacca tcattgacgt aggctcatca
      181 aatattgagc aattccttgt acagatgaat gaatatcagg gaagtcatga ccttattgat
      241 tatttcatcg ttcctgttgt gcgagaagca aaacaacaaa tagactcaat tcaaaccatt
      301 acaacactaa tggctatggg tgttgaacag gatcgtttta aagtaatttt caatcttgct
      361 gaaaaggaca cccctattaa caaacaatat tcagttttct tagctgacga cgtatgtaaa
      421 gaaattgtag gtgaaaatcc tgttgttgtt taccataaca acattttcaa tattttaaat
      481 aaaagcggcc tacagtatga tgatgtctat aatgacaatc gtgattttcg ttcattaatt
      541 cgttcagcgt cttcaaagga agagcggcaa gatttgtcaa atttaagagc agtaaaaatg
      601 ttgatgaatg gattcaacag cgatcttgat gttgccttta aaaacctcaa tctcgtctga
      661 tacaatatgg ccgggatgta cccggtcata aaattggaga aaaacaatgt ctcgtagtat
      721 ctctgaagag tttaaagatc aaaaaataaa cgatcttctt gaagattata ttgctttaga
      781 aaaacagatc gaaaaaacag caaacttaat tatcaa
//
LOCUS       DAZPLM010000444          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPLM010000444 DAZPLM010000000
VERSION     DAZPLM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.447"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHVC7_004504"
     CDS             complement(246..>815)
                     /locus_tag="ACHVC7_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2118341.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZPLM010000445          813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPLM010000445 DAZPLM010000000
VERSION     DAZPLM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.448"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHVC7_004505"
     CDS             complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHVC7_004505"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR2118342.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(553..>813)
                     /locus_tag="ACHVC7_004506"
     CDS             complement(553..>813)
                     /locus_tag="ACHVC7_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR2118343.1"
                     /translation="AAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSASATSQ
                     HLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          204 a          209 c          190 g          210 t
ORIGIN      
        1 cgcgtccggc gtcgcttctc aagctcgttg gttccacggc atcttatcta acaaccttgc
       61 cattccacaa aaaccgctga ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa
      121 taagaagaaa ccgctattta cggtataacc cagtacaacg ccgattaata ttaaaccacc
      181 cgcagcgatc tgcacctggc gcattaacgg caagggttga gatttattta ccgctactgg
      241 caatccagct cttttccagc catcaatccc atcttcgagt aaaaatattt ctgcgggggc
      301 ggcaatcgct gctaatttat cggcgttatt actggtgcgt ttacctgcct ggcagtggaa
      361 tataatttgc tcgcgacgta atttaggtgg aagacctgac tgttccagca cggataatgg
      421 agccagatct gcttcaggaa tatgttcacg aagatattca tcagcgtcac gaatatcgat
      481 taactttgcg ccgcgtgcga ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc
      541 catagtgact ccttacggac aatagacatt tttcagggtg gcgataatgg catttaccgc
      601 ctcattttta atggaatata gaatgcgttg ggcatcccgt tggctgtcga taagcccttc
      661 gtcccgcata cgagcgagat gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt
      721 cagctctccc gcgctggtgc cgggggaacc gctaagcatg cacagaatca gcaatcgttt
      781 aggatggctc attgctttca ataaggccgc tgc
//
LOCUS       DAZPLM010000446          811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPLM010000446 DAZPLM010000000
VERSION     DAZPLM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.449"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            12..>811
                     /locus_tag="ACHVC7_004507"
     CDS             12..>811
                     /locus_tag="ACHVC7_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2118344.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
BASE COUNT          231 a          160 c          199 g          221 t
ORIGIN      
        1 gggaatacat aatgaaaaga aaagttctgg caatgctggt cccagcgtta ttagttgctg
       61 gcgcagcaaa tgcggctgaa atttataata aaaatggcaa taaactggat ctgtacggaa
      121 aagttgatgc tcgtcatact ttttccgata accctggcga tgacggcgat gaaactatca
      181 tcgagttagg cttcaaaggt gagacccaaa ttacagatca gttaactggt tatggtcaag
      241 cactgaccaa aacaaaggct agcgacactg aaggttctga taatacttat gtcaaacttg
      301 cttttgcagg tctgaagttc ggcgagatgg gctcctttga ctatggtcgt aactatggtg
      361 tgatttacga cgttgaagcc tggacagata tgcttcctgt attcggcggc gactcctata
      421 cctggactga taacttcatg gcaggccgtg caaatggggt agctacttac cgcaatagtg
      481 atttcttcgg tctggtggaa ggtctgaact ttgcattgca gtatcaaggt aacaacgaag
      541 gcagcaatgc aggagaagat caagaaggta ctaaaaatgg tcacgaagac gttcgcttcc
      601 agaacggtga tggttttggt ctttccactt catatgactt tgacttcggt ttgagcttag
      661 gtgccgctta ctcaaactct gaccgtacca attctcaagt agctcttggc gggtatcact
      721 acaatgaata cagcaaattt gctggtggtg atacagctga agcatggaca tttggtgcaa
      781 aatacgacgc caacaatgtt tatcttgcaa t
//
LOCUS       DAZPLM010000447          796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPLM010000447 DAZPLM010000000
VERSION     DAZPLM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.450"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHVC7_004508"
     CDS             complement(<1..741)
                     /locus_tag="ACHVC7_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR2118345.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          160 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aatttt
//
LOCUS       DAZPLM010000448          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPLM010000448 DAZPLM010000000
VERSION     DAZPLM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.451"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHVC7_004509"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHVC7_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPLM010000449          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPLM010000449 DAZPLM010000000
VERSION     DAZPLM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.452"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHVC7_004510"
     CDS             complement(<1..458)
                     /locus_tag="ACHVC7_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2118346.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAAQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHVC7_004511"
     CDS             complement(530..742)
                     /locus_tag="ACHVC7_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118347.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          174 g          219 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgcgctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZPLM010000450          786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPLM010000450 DAZPLM010000000
VERSION     DAZPLM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.453"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..281
                     /locus_tag="ACHVC7_004512"
     CDS             <1..281
                     /locus_tag="ACHVC7_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118348.1"
                     /translation="TELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYEN
                     KEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            293..742
                     /locus_tag="ACHVC7_004513"
     CDS             293..742
                     /locus_tag="ACHVC7_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR2118349.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          262 a           88 c          195 g          241 t
ORIGIN      
        1 caactgaatt aaactcgaaa tcacaatcgc ctgcattagg tttgggacat gagtttgctc
       61 atgcgcagta ttgccttctt gataaggaaa atttcatggc tcttttaagc cgaacagata
      121 agaaatatga gaataaagaa gaggcaaggg ttattacaat aattgaatcg cgtgcggcga
      181 aaactttagg tgagtgtacc agaggagctc atagtggact gcctttttat cgggtggatg
      241 gaccgttgca gacgatgaaa ataacgggta cgccagaata gtgaggtttt ttatgatgaa
      301 atatttgatg gttttattgt ctctattttc tggttcggta ttggggatgg ggcgagtgaa
      361 tgaattgtgt ggaatagata gcgtgaaaac gatagagata attaatcttc catcgtatgt
      421 taccacgtta gttccgctta gcaaggaagg tcttaatgaa atatacagat ataaagtggt
      481 ggtgaatgaa attagagatc tttatgctgg taagataatt gatttattgc aaatgaagta
      541 tttcagaaaa gaaaaatata acaatataag atgggggggt agtatcattt cgaaaggtaa
      601 taataaatgt gaaatttatt ttgatgcttt tggtgaatgt ggaagtgtga atggcattaa
      661 cgtgtgtttt gaaaaaaatg aaatgatagg ttggattaaa aaagaaatcc ctttgctgtc
      721 gcaaaaaata ggtgggcttt aaatggtagg gcaatattca aagacagtat aggtaactat
      781 ttgtat
//
LOCUS       DAZPLM010000451          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPLM010000451 DAZPLM010000000
VERSION     DAZPLM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.454"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..623
                     /gene="yahL"
                     /locus_tag="ACHVC7_004514"
     CDS             <1..623
                     /gene="yahL"
                     /locus_tag="ACHVC7_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR2118350.1"
                     /translation="KLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVK
                     FVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEH
                     HMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLV
                     MLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          228 a          157 c          167 g          231 t
ORIGIN      
        1 acaaactcat tcatctcatg gctgccgtac gagactgtga gtggtcagac gataacgcac
       61 tcaccataaa tgtgcagttt aatgatttcc caggatttta tgactggatg gattaccctg
      121 atcacccggt taagtttgtt tttcgcatac ttgaaaatca aaaagggaca gtttgggtat
      181 atgaccagga tgatgcgttt cttgatatta aagcaaatgt gcaggctgga cgctttaccg
      241 ggcttaagaa attagtgcag tttatcaatt cagtgcgaac agattgcaaa tgtattcttc
      301 ttgaacacca tatgcctttg ctgagacttt tccctaaagg gaaagagtgt atgcatgtgg
      361 aaaaatggct tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc
      421 ttgcgcatgg attattatta cacttaaaaa atatctaccc tgtttttcct gaatcactgg
      481 tgatgttatt gctctctgtt ctggacgtaa aaacatacag agatgatgca cgattaaatg
      541 aatggatatc taatagagtg caggaattag gtgacagata ttatcctgta aataagcacg
      601 ttaaaatccg ctacactctt tagttcagaa agggttccac ctgcaagttg ttattccacc
      661 agagagtgaa tcgcaaagca ggtaatcatt tcatctgtat atccgtatgc caaatggcat
      721 tccaccgcct tttcgccgat attttgttgt gatcggcgac acttcgctta aaaaagcacc
      781 agc
//
LOCUS       DAZPLM010000452          765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPLM010000452 DAZPLM010000000
VERSION     DAZPLM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.455"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(81..>765)
                     /locus_tag="ACHVC7_004515"
     CDS             complement(81..>765)
                     /locus_tag="ACHVC7_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2118351.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQLR"
BASE COUNT          176 a          193 c          189 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatggt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAZPLM010000453          754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPLM010000453 DAZPLM010000000
VERSION     DAZPLM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.456"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>754)
                     /locus_tag="ACHVC7_004516"
     CDS             complement(<1..>754)
                     /locus_tag="ACHVC7_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR2118352.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQ"
BASE COUNT          256 a          128 c          102 g          268 t
ORIGIN      
        1 gctgttcgcc ctcataacac ttaagtaatt gatagaagta ccacttagca aaatcaggtt
       61 gcttcatgaa aaaatccttc aaaaaaatat catttactaa aatgctgaca ggcacttcat
      121 taaatagtaa cttaacatca tcaaataacc ttagtattaa gcgtgatttt tcttgattgc
      181 ttaagctaaa atcattgaca tatgcaacca acttatccca aatcaattcc tgtaatttac
      241 ggctattttt atagggttct ttatcgagat tgaaaaataa ggtctcagct attttaattt
      301 tagattgatc gcagctaaaa tctttagatt gatcgcagct aaaatcaata tgatttatca
      361 cggagcatgc atactttatc ttttcagaat caccaaaata ttgcattctt ttattaaaac
      421 accaatccag tattcttaca tcattaatat atggggattt taaaaaagtc tccagaatcg
      481 aatcgatgac cggtgtaagg ttaacttttg catcatctaa ggaatcaata agaatatgag
      541 acaggttaac tttgatgtca ttaatttcat tgtcagtagc tttcttatta ttaagaagat
      601 aattatttac tgcgttaagg tcatctattt ctttcagtat ccctttttca ccaagcaagt
      661 atgaaatcga ttcattacat acataaggca ttaatagcag atgttctttc cttattgcta
      721 ataatgcatt gttcaatggt ttttctaata aatc
//
LOCUS       DAZPLM010000454          753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPLM010000454 DAZPLM010000000
VERSION     DAZPLM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.457"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            586..>753
                     /locus_tag="ACHVC7_004517"
     CDS             586..>753
                     /locus_tag="ACHVC7_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR2118353.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          214 a          135 c          165 g          239 t
ORIGIN      
        1 agcttgcttg caccgtattg ttcttgtttt tcggttagtt aaacatcgta cttcatattt
       61 gaacgttctg ccggaatgca ttatcaatag aggtaaagtc gcaaccccaa atcgtaaagg
      121 aaaccgtagc acgtcgtatg caagaacgtg ccacggctgg ctgatggatg ttcgatagcg
      181 cgagtttgaa tgaaaatcag ccggagatga ttttacataa ttgctacgga attattcaat
      241 acaggaattg cttgcgtctg catggattga cctgaaatat tcccgaaaat ttctctaaaa
      301 aactcgaaaa aaatggtaac tggttgaatg tattaatatg caatggtacg tgtcagggat
      361 taaaagatga acgtaaattt atttaacgca ttaattttaa agggttttat tgtttgttga
      421 cgaaaacagg aatcgtgttc ggtctctttt tatctgttaa aagccagaag catttccttc
      481 gctgacttta tagtcaacca taacacacac tctactgtct gagtccaacg ttttttaaca
      541 ttcttgttaa aattatgtga tctttagcgc gggaggaaaa tattgatgaa acagcctgcg
      601 cccgtttatc agagaattgc gggtcatcaa tggcgacata tctggctttc tggcgatata
      661 cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt gtcgttttga tccgtggcga
      721 gatttactta tctcagtggg agacgttatc gat
//
LOCUS       DAZPLM010000455          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPLM010000455 DAZPLM010000000
VERSION     DAZPLM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.458"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>750
                     /gene="gspA"
                     /locus_tag="ACHVC7_004518"
     CDS             <1..>750
                     /gene="gspA"
                     /locus_tag="ACHVC7_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR2118354.1"
                     /translation="RREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKHRRV
                     ITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIE
                     NAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHELK
                     ASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQ
                     WRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          196 a          177 c          193 g          184 t
ORIGIN      
        1 cgagaagaga agttattctt tcctggttgc gtgagaaacg tcagacctgg cgtctgtgct
       61 atttgttggg tgaggctgga agtggaaaaa cctggctggc gcagcaactg caaaaagata
      121 aacatcgccg tgtgattact ttaagcctcg ttgtttcctg gcaaggtaag gccgcatgga
      181 tcgttaccga cgataacgcg gctgaacagg gctgccgtga cagcgcctgg acgcgagatg
      241 agatggcggg gcaattactg catgcgcttc atcgaactga tagtcgctgc cctcttatta
      301 ttattgaaaa tgctcacctg aatcaccgcc gcatacttga tgatctccaa cgcgcgatat
      361 ctcttattcc tgacggtcaa tttttgctga tcggcagacc cgatcgaaaa gtcgaacgtg
      421 attttaaaaa acagggcatt gaacttgtct cgacaggacg tctgacggag cacgaactta
      481 aggcaagcat ccttgaaggt cagaatattg accaacccga tctcctttta acctccagag
      541 ttctgaaacg gatagcttta ttatgtcggg gcgatcgcag aaagctggcg cttgccggcg
      601 aaacaattcg cttattacag caagccgagc agaccagcgt tttcactgca aaacaatggc
      661 gaatgattta ccgcatattg ggtgataacc gcccccggaa aatgcagctt gccgtggtaa
      721 tgagtggaac cattatcgct attacctgtg
//
LOCUS       DAZPLM010000456          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPLM010000456 DAZPLM010000000
VERSION     DAZPLM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.459"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHVC7_004519"
     CDS             <1..256
                     /locus_tag="ACHVC7_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HFR2118355.1"
                     /translation="DFFIKKKTIEQEVKKTNVEGQIETNKEEIEKVTFHWRNALGFVL
                     TLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            317..>750
                     /locus_tag="ACHVC7_004520"
     CDS             317..>750
                     /locus_tag="ACHVC7_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2118356.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHI"
BASE COUNT          255 a          121 c          142 g          232 t
ORIGIN      
        1 ggattttttc attaagaaga aaactattga acaggaagta aagaaaacga atgtagaagg
       61 gcagattgaa acgaataaag aagagattga aaaggtaaca ttccactggc gtaatgccct
      121 cggttttgta ctaaccctta tcattctcta taactggatc atagtaccag tacttgatgc
      181 ttttggaatt attgtgatcc aagttccatt aggacaactt ttgcaagtgc tattgattat
      241 ggttggtgga agctgaaaga tttttccttt aatatgtgtt ttgagttaat aaaatagcaa
      301 tctaaggatt tgatttatga gtgatgcaat tactacagca gcaataaaaa aattgtatgg
      361 actatccgct gggcaatgta atatttgccg aacttctctt tttaaagagc aggttcacat
      421 cggacaaatg gctcatgtaa tagccaaaag cccaaaagga ccaaggggcg atgacctgtt
      481 agcaaatgac aattcttatg aaaatcttat cctgctttgt gcaaatgatc atattcttgt
      541 tgatagagat ccattcactt atacagtaga aaaattgcat caaatcaaaa gtgaacatga
      601 ggcttatata cagggtgcaa ccgataatac attattttca gataaaaaaa gacagtcaga
      661 tgtggacttt ttaaacgctt acttccactt tacaccgttt gcgagggtac gttcattagt
      721 tgaaacttta cctaattctt ttcacatcaa
//
LOCUS       DAZPLM010000457          749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPLM010000457 DAZPLM010000000
VERSION     DAZPLM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.460"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>749
                     /locus_tag="ACHVC7_004521"
     CDS             <1..>749
                     /locus_tag="ACHVC7_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2118357.1"
                     /translation="PGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAA
                     AKKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAS
                     KSAAAAESSKSAAATSAGAAKTLETNAAASQQSAATSASTATTKASEAATSARDASAS
                     KEAAKSSETNASSSASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKT
                     AAASSASAASTSAGQASASATAAGKSAESAA"
BASE COUNT          185 a          234 c          227 g          103 t
ORIGIN      
        1 acccggtacg ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc
       61 actgcgccgt tttgagctga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca
      121 gaacacggca gccgcgaaga aatcagccag cgatgccagc acatcagccc gtgaggcggc
      181 aacccatgcg actgatgctg cagattcagc acgtgcagcc agcacgtcag ccggacaggc
      241 cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa aggccactga
      301 agcctcaaaa agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg
      361 tgcggcgaaa acgttagaaa cgaatgcggc agcgtcacaa caatcagcag ccacttctgc
      421 atccaccgcg accacgaaag cgtcagaagc tgccacctca gcccgggatg cgtcggcttc
      481 aaaagaggcg gcaaaatcat cagaaacgaa cgcatcatca agcgccagta gcgcagcttc
      541 ctcggcgacg gcggctgcaa attctgcgaa ggcggccaaa acgtccgaga cgaacgccag
      601 gtcttctgaa acggcagcgg gacagagtgc ctcagctgcg gcaggctcaa aaacagcggc
      661 tgcgtcgtct gccagcgccg cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc
      721 cgccggaaaa tcggcagaaa gcgccgcat
//
LOCUS       DAZPLM010000458          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPLM010000458 DAZPLM010000000
VERSION     DAZPLM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.461"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHVC7_004522"
     CDS             <1..407
                     /locus_tag="ACHVC7_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2118358.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="ACHVC7_004523"
                     /pseudo
     CDS             449..649
                     /locus_tag="ACHVC7_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZPLM010000459          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPLM010000459 DAZPLM010000000
VERSION     DAZPLM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.462"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHVC7_004524"
     CDS             <1..400
                     /locus_tag="ACHVC7_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR2118359.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          261 a          138 c          141 g          206 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgt
//
LOCUS       DAZPLM010000460          741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPLM010000460 DAZPLM010000000
VERSION     DAZPLM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.463"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>741
                     /locus_tag="ACHVC7_004525"
     CDS             <1..>741
                     /locus_tag="ACHVC7_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2118360.1"
                     /translation="EAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPD
                     VVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQ
                     QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDP
                     LGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSV
                     DLRNIKCPEGSERVGDYHTHGFYSDDKG"
BASE COUNT          220 a          154 c          209 g          158 t
ORIGIN      
        1 tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag
       61 tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc ggacaacaga
      121 atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg atggcagggc
      181 atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct
      241 ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga acccgcatca
      301 gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg gcctgtatta
      361 taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg
      421 gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata gcatggatcc
      481 attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat ttgcattggc
      541 aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa tatgtaagaa
      601 gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata gtgtagactt
      661 gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca ctcacggttt
      721 ttactctgac gataaaggaa a
//
LOCUS       DAZPLM010000461          738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPLM010000461 DAZPLM010000000
VERSION     DAZPLM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.464"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>738
                     /locus_tag="ACHVC7_004526"
     CDS             <1..>738
                     /locus_tag="ACHVC7_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2118361.1"
                     /translation="YNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETAT
                     ELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLR
                     HVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNP
                     VSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLHQRLFRCVSIRENDDGTYAITA
                     VQHVPEKEAIVDNGAHFDGDQSGTVNG"
BASE COUNT          155 a          194 c          251 g          138 t
ORIGIN      
        1 cctataaccg cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagtttca
       61 gcgccctgaa agaccgccat aatgccgttg aggtgaactg gattgacccg gataacggct
      121 gggagacggc gacagagctt gtggaggata cgcaggccat tgtccgttac ggtcgtaacg
      181 tcacgaagat ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt
      241 ggctgattaa aacggaactg ctggagacgc agaccgtgga tttcagcgtg ggtgctgaag
      301 ggcttcgtca tgtaccgggc gatgtcattg aaatctgcga tgatgactat gccggtatcc
      361 gcaccggcgg gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg
      421 aaatcacgct gccatcctcc ggcaccacgc tgataagcct ggttgacggg caggggaatc
      481 cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc
      541 ctgacggtgt tgctgaatac agcgtgtggg ggctgaagct gccgacgctg caccagcgat
      601 tgttccgctg cgtgagtatc cgtgagaacg acgacggcac gtatgccatc accgccgtgc
      661 agcatgtgcc ggaaaaagaa gccatcgtgg ataacggggc gcactttgac ggcgaccaga
      721 gcggcacggt gaatggtg
//
LOCUS       DAZPLM010000462          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPLM010000462 DAZPLM010000000
VERSION     DAZPLM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.465"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHVC7_004527"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHVC7_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZPLM010000463          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPLM010000463 DAZPLM010000000
VERSION     DAZPLM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.466"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>730)
                     /locus_tag="ACHVC7_004528"
     CDS             complement(<1..>730)
                     /locus_tag="ACHVC7_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118362.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYG"
BASE COUNT          121 a          240 c          202 g          167 t
ORIGIN      
        1 agccgtagtg gaccgccaca cggtggcctt cactcaggtg gctgatttct gtcagccagc
       61 cgtgatcgtc atactgccac tgctccgccg gttcaccgtt caccgtgcgc cgggtgatac
      121 ggtccgacgc atcgtaatgc cagagggtga taagcccctc atcttcactc tgcgtgagtt
      181 ttccggtcag gtcatactgg taacgttgcg tccggccgtc aaagccgcgc tgctccgtca
      241 gcctgtcgac cgggtcatac cggaacgtgg actggctgcc gttctcgttg gtcagcacag
      301 tgatgcgccc ggcggcgtca taccccatgc ttcgcgtcag accgccctgc gtggtgctga
      361 cggcctttcc ccacgcatca tactgtatct cactgcggct gccgtccggg gcaacgatag
      421 cggtgaggtc tcctgcggcg ctgtactcat aacgggtttc acgcccctgc gcatccctct
      481 gactgaccag ttgaccgcgc gggttataac tgctgtaagt gctgatgcct tcttcccggt
      541 gaacggcgat ttgttgaccg taccggtcat actcataccg cgtcgtgtac cccgagcagt
      601 ccgtaaacgc ctgaagctga ccgtaccggt ttcgcgttat tctccgcctc accccggttc
      661 cgtcttccac cgcacagggc aggccactgc gggaagaatc ataaaaccag cgcgtaatgt
      721 taccgttgcg
//
LOCUS       DAZPLM010000464          728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPLM010000464 DAZPLM010000000
VERSION     DAZPLM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.467"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            64..>728
                     /locus_tag="ACHVC7_004529"
     CDS             64..>728
                     /locus_tag="ACHVC7_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118363.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGANFIDFNM
                     TGQ"
BASE COUNT          288 a          141 c          108 g          191 t
ORIGIN      
        1 ttcctgttct aattatccaa tttaaacatc ttagcataaa acaaatgatg aataaggaat
       61 tttatgtcaa ttgactttac cccaggtata ataaatacat atcacggcga tatttataac
      121 tgcacaacaa ataccgataa tgctaaaacg ccagacacac cgaagtggcc ttgcgataat
      181 tgggaagaac aacaacccat taattccaca ttctctggag aaggatatat ctctgatcaa
      241 tatgatttag tgcaacacca acttcaacaa attaatgcgt gccacacgaa cactacatat
      301 acaaatgcag actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa
      361 acgataagtt cgacacaact tacgcaacag acacaatcaa tacttaatca gattaataac
      421 attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa
      481 cccgacaagg caatcataac taaaattagc gtagaagagg gaatacccat aacattcagt
      541 gtccaaacca tgttttcaga tactaatttt attgctgaac aacgagctga cttaccaact
      601 aatataaaag atattcaaag cctgtatcaa aaaatgacga agttatatat tgagcacagt
      661 gaaaataaaa acaggatgaa agtctttgcc ggagccaact ttattgattt caatatgaca
      721 ggacagaa
//
LOCUS       DAZPLM010000465          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPLM010000465 DAZPLM010000000
VERSION     DAZPLM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.468"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHVC7_004530"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHVC7_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR2118364.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPLM010000466          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPLM010000466 DAZPLM010000000
VERSION     DAZPLM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.469"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            2..>705
                     /gene="istB"
                     /locus_tag="ACHVC7_004531"
     CDS             2..>705
                     /gene="istB"
                     /locus_tag="ACHVC7_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR2118365.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQG"
BASE COUNT          211 a          158 c          202 g          134 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggacaaagc agcacgatag tggtaagcca
      601 gttcccggta gataaatggc atggtctgat ggagaaccca acaacagcag atgccatcct
      661 ggacagactg gtacataact cacacagagt ggtccttcag ggaga
//
LOCUS       DAZPLM010000467          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPLM010000467 DAZPLM010000000
VERSION     DAZPLM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.470"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
BASE COUNT          217 a          172 c          135 g          180 t
ORIGIN      
        1 gttgatattt tttccatctg atacatatcc cgccccccga cagacctgtg ctatcggaaa
       61 gaaaaaaggc catcagcaaa actctgatgg cctgaatcac ctttaccaat attgtatgaa
      121 aaaacacgca cgactaattg acaataattt tcatttccat taaaaaagat cgcgtagcat
      181 tcttaattca tgaggaactt tacccgccag caatctgagt agcgaaagct gttcatcccc
      241 aatgtttttg ctggcgggtc cttttttcta cggtcctcct ccccggaggg gggcatttca
      301 ttatttttct aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa
      361 ataacagcat gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcgct
      421 gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc ccggggtttg cttagaatat
      481 tttgtaagtt gtccgaagag atatttacaa caccagaatg atgaatcatc agtcccctgc
      541 cagaggttca ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac
      601 tggaactgca acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac
      661 acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa tctc
//
LOCUS       DAZPLM010000468          702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPLM010000468 DAZPLM010000000
VERSION     DAZPLM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.471"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            23..>702
                     /locus_tag="ACHVC7_004532"
     CDS             23..>702
                     /locus_tag="ACHVC7_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2118366.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          221 a          150 c          126 g          205 t
ORIGIN      
        1 aatatccttc tgcgaggtta ttatgcttcc tgtaaataat ccccccctat ccactggaaa
       61 cgtctctttt tacagaacta catcaatcga caatgttcac aataattatc tctccgaatg
      121 ggttgaatgg actaaaaaca gcatttccgg agaaaacagg gaaactgctt ttacccggct
      181 ccaattatgt ctggagaaca gtgaaacatc gttggactta tcttgtttag gtctcagatc
      241 tctaccacga ttgcctgaca atcttgatga aattaatgta agcaataacc aactatcaat
      301 gctccccgag ctaccaaggg cattgaaaga gctgaatgca agcagtaatc aattatctgc
      361 acttcctgaa ttaccagtgt cgctggaata tataaatgtg agtgataacc atttgttcgc
      421 acttcctgaa ttacctgcgt cactagaata tattaatgta agtgacaatc acctgtctgt
      481 acttccgagg ttaccaatgt cattggaatt acttgatgca gccagaaatg ctttggaagt
      541 aataccagat tttccagaaa gagatgatca tattataaga atattctggc ttaatcagaa
      601 ccggatcacg gcaattccgg aaagcatact tggcctcagt tctgatagcg ttgtcaatct
      661 tagagaaaat caactatctc ccagaataat gcaaactttg tt
//
LOCUS       DAZPLM010000469          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPLM010000469 DAZPLM010000000
VERSION     DAZPLM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.472"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHVC7_004533"
     CDS             <1..>698
                     /locus_tag="ACHVC7_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118367.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZPLM010000470          691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPLM010000470 DAZPLM010000000
VERSION     DAZPLM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.473"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHVC7_004534"
     CDS             complement(<1..304)
                     /locus_tag="ACHVC7_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR2118368.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          215 a          156 c          155 g          165 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg a
//
LOCUS       DAZPLM010000471          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPLM010000471 DAZPLM010000000
VERSION     DAZPLM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.474"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPLM010000472          688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPLM010000472 DAZPLM010000000
VERSION     DAZPLM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.475"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            54..596
                     /locus_tag="ACHVC7_004535"
     CDS             54..596
                     /locus_tag="ACHVC7_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118369.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          219 a          121 c          144 g          204 t
ORIGIN      
        1 ccaagagata cagactctta acaaccaaaa aaaattttat taaggaacat attatgaaac
       61 gttcaattat tgctgccgct gtcttttctt ctttttttat gagcgctgga gtatttgctg
      121 cagacgttga taccggaaca ttaactatta aggggaatat tgcagaatct ccgtgtaaat
      181 tcgaagcggg tggtgattca gtaagtatta atatgccgac tgtaccaacc actgtctttg
      241 aaggtaaagc taaatattct acctatgatg atgcagtcgg tgtaaccagc agcatgttaa
      301 aaattagctg cccgaaagaa gttgctggtg taaaactctc gttgattacc aacgataaaa
      361 taaccggtaa cgataaggcg atagccagta gcaacgatac cgtgggttac tatctctatt
      421 taggtgataa cagcgatgtc ctggatgttt ctgcaccttt taacattgag agttataaaa
      481 cagcggaagg tcaatatgct attccgttta aagcaaaata cctgaaactg acagataact
      541 cagtgcaatc aggtgatgtg ttatcttctc tggttatgcg tgtggcgcag gattaataaa
      601 taaaattgta agtttctcca ggtaataaac atggccgcta tcccatggcg gccttttaat
      661 ttaagaggca ttaaaatgaa aggattat
//
LOCUS       DAZPLM010000473          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPLM010000473 DAZPLM010000000
VERSION     DAZPLM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.476"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..683
                     /locus_tag="ACHVC7_004536"
     rRNA            <1..683
                     /locus_tag="ACHVC7_004536"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          151 c          230 g          146 t
ORIGIN      
        1 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
       61 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      121 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      181 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
      241 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
      301 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
      361 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
      421 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
      481 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
      541 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
      601 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
      661 aaccggtact aatgaaccgt gagg
//
LOCUS       DAZPLM010000474          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPLM010000474 DAZPLM010000000
VERSION     DAZPLM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.477"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHVC7_004537"
     CDS             <1..560
                     /locus_tag="ACHVC7_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR2118370.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHVC7_004538"
     CDS             complement(616..>684)
                     /locus_tag="ACHVC7_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118371.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPLM010000475          683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPLM010000475 DAZPLM010000000
VERSION     DAZPLM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.478"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>683
                     /locus_tag="ACHVC7_004539"
     CDS             <1..>683
                     /locus_tag="ACHVC7_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR2118372.1"
                     /translation="DDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKR
                     VYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFA
                     LDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANE
                     TRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFE
                     HYNEWHPHS"
BASE COUNT          169 a          163 c          209 g          142 t
ORIGIN      
        1 ctgatgatac ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg
       61 gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga
      121 tcaatgccaa acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac
      181 ctgctgtacc gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc
      241 agcgatggtg ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca
      301 cgttcgcgct ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct
      361 tcaacagtga aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg
      421 atcttccgtc gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg
      481 aaacacgcca gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc
      541 cggagagtaa cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta
      601 tcatgcccaa accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt
      661 ataacgaatg gcatccgcat agt
//
LOCUS       DAZPLM010000476          674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPLM010000476 DAZPLM010000000
VERSION     DAZPLM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.479"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(55..351)
                     /locus_tag="ACHVC7_004540"
     CDS             complement(55..351)
                     /locus_tag="ACHVC7_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR2118373.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          200 a          145 c          141 g          188 t
ORIGIN      
        1 ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattgaatct ctattcagaa
       61 cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta cgtacgggtc
      121 agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc
      181 agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac cagctgtcaa
      241 caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag
      301 gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac
      361 tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa
      421 gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa aatgacattt
      481 tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag
      541 acttggtttt tctactgaaa tgattatgac ttcaatgacc cttgaatgaa gtctattcgg
      601 ttttctagga gacctgttaa aaacaggaca atatcaggtg agtccctatg caatttaata
      661 gtagatttcc aagt
//
LOCUS       DAZPLM010000477          673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPLM010000477 DAZPLM010000000
VERSION     DAZPLM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.480"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            2..478
                     /locus_tag="ACHVC7_004541"
     CDS             2..478
                     /locus_tag="ACHVC7_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2118374.1"
                     /translation="MEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAFD
                     YHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPYD
                     DYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          249 a          103 c          107 g          214 t
ORIGIN      
        1 aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg cattatcgaa
       61 aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata gaatatatgc
      121 atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt attataaatt
      181 tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat cagtatgtca
      241 gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca attactcccc
      301 gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg ctaaaaatat
      361 cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg atagtgttaa
      421 gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata atggctaagc
      481 attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat aaccagcacc
      541 tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata aatcattatt
      601 tttcaatgtg ttagagaaca atctacttta taaaaattga ataaggctat ttcagattac
      661 atcactgaat tcc
//
LOCUS       DAZPLM010000478          672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPLM010000478 DAZPLM010000000
VERSION     DAZPLM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.481"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>672
                     /locus_tag="ACHVC7_004542"
     CDS             <1..>672
                     /locus_tag="ACHVC7_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2118375.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNG"
BASE COUNT          155 a          163 c          242 g          112 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac gg
//
LOCUS       DAZPLM010000479          671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPLM010000479 DAZPLM010000000
VERSION     DAZPLM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.482"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>671)
                     /locus_tag="ACHVC7_004543"
     CDS             complement(<1..>671)
                     /locus_tag="ACHVC7_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR2118376.1"
                     /translation="TLTKLEKQTYPNFEIVIVNDGSKDNTSNVLREYGLTHSRLIIIN
                     KENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMYTRQHETGGDAIYCGLYGH
                     YIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGCLFIRKQLLEENNLFFDEDLRLGE
                     DLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHYRHESFAHERIYSSVMQL
                     YKGNR"
BASE COUNT          214 a          131 c           99 g          227 t
ORIGIN      
        1 cctgttacct ttgtataact gcatcacaga agagtagatt ctttcgtgcg caaatgactc
       61 atgtcgataa tgagtgatgg ttctacatga gttcattaag gaattttctc tatagttatg
      121 cttatacata taatatggaa ccgcatacat atcgcatgta attagcagtc gatagataaa
      181 atccagatct tctcctagtc gtaaatcttc atcaaaaaaa agattattct cttccagaag
      241 ttgttttctt ataaacaagc accctatatg gaatctaacc tttttataaa aaaagtcgaa
      301 aagtaaagat ccttcattaa actctgtatt tataggtgag taagtaacac catcttttat
      361 ataatggcca taaagcccac aataaatcgc atcccctccc gtctcatgtt gtctggtata
      421 catcttcagc agatagttag gatctatctc atcatcatca tccataaaac atataaactg
      481 tccttgcgcc ttgcggatac ctgtattcct ggcagacgaa acaccgccat tttctttatt
      541 gataataatt aatcgagagt gggttaaccc atactctctc aaaacgtttg atgtgttatc
      601 tttagaacca tcattaacaa taacaatttc aaaatttggg taagtttgtt tttctaattt
      661 cgttagagtt t
//
LOCUS       DAZPLM010000480          667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPLM010000480 DAZPLM010000000
VERSION     DAZPLM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.483"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>667
                     /locus_tag="ACHVC7_004544"
     CDS             <1..>667
                     /locus_tag="ACHVC7_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR2118377.1"
                     /translation="KYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKG
                     SLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEAN
                     LTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRK
                     YQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQK
                     QYKH"
BASE COUNT          208 a          131 c          133 g          195 t
ORIGIN      
        1 aaaatacttt gctgatctgc cgacgtcaca gttttggtcg tacgcgacat acttcagggt
       61 attgtccttt gaatatctga gtgaaagtat ttccacactg ctgtatctgg atgccgatgt
      121 cgtttgtaaa ggaagtctga agcctctcac agaaattata tttaaagatg agtttgctgc
      181 ggtcattcct gacaatgtta gtactcaggc ggcatgtgca aaacgcctca acattcccga
      241 gatgaatgga cgttatttca atgcaggcgt tatctatgtc aatcttaaaa aatggcatga
      301 agcaaatttg acaccgtatt tactcacgct tttacgaggg gaaactaaat atggctctct
      361 taaatattta gatcaggatg cgttgaatat cgcatttaat atgaataata tctacctcgc
      421 gaaggatttt gatactattt ataccctgaa aaacgaactt catgatcgta gtcatcgaaa
      481 gtatcagcaa accattaccg ataaaacagt attgattcac tatacaggga taactaaacc
      541 atggcatagc tgggctggtt atccgtctgc atcatacttt aatatcgcgc gtgaacaatc
      601 tccctggaag aaatatcctc ttaaagaggc gcggactgtt gcagaaatgc agaaacaata
      661 taagcat
//
LOCUS       DAZPLM010000481          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPLM010000481 DAZPLM010000000
VERSION     DAZPLM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.484"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHVC7_004545"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHVC7_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          228 a          120 c          123 g          194 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttctgcccc ctgtcaactc aatctgccaa taaataacat
      661 aatta
//
LOCUS       DAZPLM010000482          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPLM010000482 DAZPLM010000000
VERSION     DAZPLM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.485"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>662
                     /locus_tag="ACHVC7_004546"
     CDS             <1..>662
                     /locus_tag="ACHVC7_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2118378.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 tg
//
LOCUS       DAZPLM010000483          653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPLM010000483 DAZPLM010000000
VERSION     DAZPLM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.486"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            88..606
                     /locus_tag="ACHVC7_004547"
     CDS             88..606
                     /locus_tag="ACHVC7_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR2118379.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          179 a          150 c          139 g          185 t
ORIGIN      
        1 atgacgatcg catgaaaaaa tgatatttta atgagcattc tgaaaaaata tcgatgcatt
       61 tcgagcgaag atggtgggga tccctgaatg cgtttttctc accgactgtt cttacttctg
      121 attctcctgc tgacgggagc accaatactc gctcaggagc cttctgacgt tgcgaaaaac
      181 gtgcgtatga tggtttccgg tattgtcagt tacacccgct ggcctgcgct atcagggcca
      241 ccaaagttat gtatcttttc atcttcacgt tttagtaccg ccctacaaga gaatgccgcg
      301 acatctttgc cttatctgcc tgtcatcatt cacactcaac aagaggcgat gatttcaggc
      361 tgcaatggtt tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa
      421 tatccgtcaa aggcgttatt attaattgcc gaacaaaata ccgagtgcat tattggtagc
      481 gccttttgtc tgatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctta
      541 tcgcgtagcg gtgtaaaggt caacccggat gtcttaatgc tcgcacggaa gaaaaatgat
      601 ggataacgat aattctctta ataagcgccc cacgtttaaa agagcattac gca
//
LOCUS       DAZPLM010000484          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPLM010000484 DAZPLM010000000
VERSION     DAZPLM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.487"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHVC7_004548"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHVC7_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHVC7_004549"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHVC7_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          154 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaacg
//
LOCUS       DAZPLM010000485          648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPLM010000485 DAZPLM010000000
VERSION     DAZPLM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.488"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHVC7_004550"
     CDS             <1..194
                     /locus_tag="ACHVC7_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR2118380.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="ACHVC7_004551"
     CDS             complement(195..386)
                     /locus_tag="ACHVC7_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR2118381.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>648)
                     /locus_tag="ACHVC7_004552"
     CDS             complement(491..>648)
                     /locus_tag="ACHVC7_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR2118382.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          183 a          147 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggtta
//
LOCUS       DAZPLM010000486          643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPLM010000486 DAZPLM010000000
VERSION     DAZPLM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.489"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>643
                     /locus_tag="ACHVC7_004553"
     rRNA            <1..>643
                     /locus_tag="ACHVC7_004553"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          181 a          140 c          200 g          122 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gcc
//
LOCUS       DAZPLM010000487          642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPLM010000487 DAZPLM010000000
VERSION     DAZPLM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.490"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHVC7_004554"
     CDS             <1..297
                     /locus_tag="ACHVC7_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR2118383.1"
                     /translation="AQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPG
                     MTVETARRILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            307..639
                     /locus_tag="ACHVC7_004555"
     CDS             307..639
                     /locus_tag="ACHVC7_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR2118384.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
BASE COUNT          145 a          191 c          194 g          112 t
ORIGIN      
        1 gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagtcgcatt
       61 atggggatcc tcaactgtga ggaggctcac ggacgcgaag aacaggcacg cgtgctggcc
      121 gaaacccccg gtatgaccgt ggaaacggcc cgccgtattc tggccgcagc accacagagt
      181 gcacagacgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg
      241 gctgcaggta acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg
      301 atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa tagtgacccg
      361 gctcataccg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gaccccgctg
      421 atgctggaca cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc
      481 gttggcattc ttgcggttgc tgctgaccag accagcacca cactgacgtt ctacaagtcc
      541 ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa
      601 cggaccgtgt ttgccggaac ggcaatcagc atcgtttaac tt
//
LOCUS       DAZPLM010000488          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPLM010000488 DAZPLM010000000
VERSION     DAZPLM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.491"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHVC7_004556"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHVC7_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR2118385.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPLM010000489          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPLM010000489 DAZPLM010000000
VERSION     DAZPLM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.492"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>640
                     /locus_tag="ACHVC7_004557"
     CDS             <1..>640
                     /locus_tag="ACHVC7_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR2118386.1"
                     /translation="YNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQL
                     CSLPVLPELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDN
                     MLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTS
                     SPDYHGPQIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          193 a          161 c          106 g          180 t
ORIGIN      
        1 gctataacag acttattgaa ctgcctgctt tgcctcaagg acttaaatta ttgaatgcgt
       61 cccacaatca actaatcaca ctacccacac tccccatatc tttgaaggag cttcatgtct
      121 caaataatca attatgttct cttcctgttt taccagaact actggaaaca ttagatgtat
      181 catgtaatgg gctggcagtt ttaccacctt taccattttc tttacaagag attagcgcaa
      241 tagggaatct tcttagtgaa ctcccccctc tacctcacaa cattcactcc atatgggcaa
      301 tcgacaatat gttaaccgat attccatacc tgccggaaaa tttaaggaac ggttattttg
      361 acataaatca gataagtcat atcccggaaa gcattcttaa tctgaggaat gaatgttcaa
      421 tagatattag tgataaccca ttgtcatccc atgctctgca atccctgcaa agattaacct
      481 cttcgccgga ctaccacggc ccgcagattt acttctccat gagtgacgga caacagaata
      541 cactccatcg ccccctggct gatgccgtga cagcatggtt cccggaaaac aaacaatctg
      601 atgtatcaca gatatggcat gcttttgaac atgaagagca
//
LOCUS       DAZPLM010000490          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPLM010000490 DAZPLM010000000
VERSION     DAZPLM010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.493"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHVC7_004558"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHVC7_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZPLM010000491          632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPLM010000491 DAZPLM010000000
VERSION     DAZPLM010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.494"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHVC7_004559"
     CDS             complement(<1..140)
                     /locus_tag="ACHVC7_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR2118387.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHVC7_004560"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHVC7_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR2118388.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT          226 a          137 c          108 g          161 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat at
//
LOCUS       DAZPLM010000492          628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPLM010000492 DAZPLM010000000
VERSION     DAZPLM010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.495"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHVC7_004561"
     CDS             <1..547
                     /locus_tag="ACHVC7_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118389.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          175 a          127 c          191 g          135 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggc
//
LOCUS       DAZPLM010000493          622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPLM010000493 DAZPLM010000000
VERSION     DAZPLM010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.496"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHVC7_004562"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHVC7_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          203 a          140 c          112 g          167 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataat
      541 ttccatggtt atttctaggc tatttaaatt aaataaataa attatgagtc aatcataaat
      601 aacaaccttt aatataattt aa
//
LOCUS       DAZPLM010000494          611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPLM010000494 DAZPLM010000000
VERSION     DAZPLM010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.497"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHVC7_004563"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHVC7_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHVC7_004564"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHVC7_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          206 a          119 c          121 g          165 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct g
//
LOCUS       DAZPLM010000495          599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPLM010000495 DAZPLM010000000
VERSION     DAZPLM010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.498"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHVC7_004565"
     CDS             38..580
                     /locus_tag="ACHVC7_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2118390.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT          178 a          118 c          136 g          167 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgttt
//
LOCUS       DAZPLM010000496          598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPLM010000496 DAZPLM010000000
VERSION     DAZPLM010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.499"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(57..>598)
                     /locus_tag="ACHVC7_004566"
     CDS             complement(57..>598)
                     /locus_tag="ACHVC7_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118391.1"
                     /translation="EILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGAL
                     SESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLA
                     SFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDK
                     WKRENMTPFMYVLNTPPVI"
BASE COUNT          193 a          129 c           98 g          178 t
ORIGIN      
        1 ataaaaaaca tcgaaagaaa ctcttttaat ttaacatgta aacgcatggt taatcctcat
       61 atcacgggtg gagtgttaag aacatacata aatggagtca tgttttccct tttccattta
      121 tcaagttcct gttgccgttt tagtccatct ctaattgcat attttaattt ttctgataaa
      181 tggcattgag catcgatttc atttaaaaca actgtacaca ctatccctgt cagtaaaatt
      241 gctcctccga caagaattac tggaaagcca aagctaacac aggcaaaaga agccaggctt
      301 ccaatagcgg ttgcaacacc accggctata actgctttgg ccacatccat tgaaaaatcg
      361 ccaagaaaat taaccagatc tcgctctgaa ctcataataa attctatggt tctccaggct
      421 actgaaaacc agatacagaa cttaactcct tttattgatt cagataaagc tcctcgccag
      481 ccaatagcga gttccagaac ttgaggcgct cgtgaagaga aaatatattt accgagcttt
      541 tctaaaagcc aggggctgga tgttgtgatt tttatatatt tctctccaag tatttcaa
//
LOCUS       DAZPLM010000497          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPLM010000497 DAZPLM010000000
VERSION     DAZPLM010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.500"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHVC7_004567"
     CDS             <1..91
                     /locus_tag="ACHVC7_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118392.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHVC7_004568"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHVC7_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR2118393.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPLM010000498          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPLM010000498 DAZPLM010000000
VERSION     DAZPLM010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.501"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(357..>585)
                     /locus_tag="ACHVC7_004569"
     CDS             complement(357..>585)
                     /locus_tag="ACHVC7_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR2118394.1"
                     /translation="GQCQSGVWRALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSH
                     YCQVVESPSGSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          145 a          143 c          139 g          158 t
ORIGIN      
        1 ggccagtgcc aatccggtgt gtggagaact tccggttcct ctaacggtag ttacagtaat
       61 cttggatcac accgaggatc atttactggt cgcaatacgg gaagtggtac gctgtttgtt
      121 tacgcctctg gagggaatgg tggttccgcc gggggagatt gtgctaacac atccagattg
      181 cagggatacg ttgcaggggc attgattagc acaaatgcga gtaataaccc atcttatggg
      241 aagacagcat tcatctcttt tgctgtccct gctggcgcaa cttatcagat cacctcttat
      301 ccggcccaaa actactcttg tggctcaggg gtattttcag tatttggata ccaaacttaa
      361 ttgagagtta cacaggacgc aatacaacct gtcttgtgct cgtatttgta ccattttcgt
      421 gaaccagatg gactctcgac tacctggcag taatgagaat cctcagcatt tcccattctg
      481 gcaatggcac aaaagtcaaa ttgccccaag taaccagtgc tggaaagttg agtaaccttg
      541 agctttccac ctaatgccct ccacacaccg gattggcact ggcct
//
LOCUS       DAZPLM010000499          578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPLM010000499 DAZPLM010000000
VERSION     DAZPLM010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.502"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>578
                     /gene="espX1"
                     /locus_tag="ACHVC7_004570"
     CDS             <1..>578
                     /gene="espX1"
                     /locus_tag="ACHVC7_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR2118395.1"
                     /translation="TLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYP
                     QLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYN
                     EQCNKIPSSSIDDNQGIMWQNFKTSNSAYSKLCGKLSLFKSVPSRLIEKSAYCSTENM
                     ATNKFNVVFSYCGDNIKEFILLLPYNKSLEMY"
BASE COUNT          225 a          101 c           88 g          164 t
ORIGIN      
        1 cacattactt agattggatg tttttgatga cagaacaagc gaatgtcttt ctgaagagat
       61 taagatatac caagaatgcc atgaaaaatt cgttaatttt ttaaaggcaa acttcaacca
      121 agaaatatac ccacagctat atactcctga aatattttat gaggcctgca gaaatctcca
      181 gtcattttat gatcatcagg aaacgtcaca aaacgcaaaa tacagtgcaa tagacaagaa
      241 aaaaagtcat cttaaccagg aaatcaaaaa gcttattaaa aaaaatattt tccctgagct
      301 atacaatgag caatgcaata aaatcccctc ctcatcgata gatgataacc agggaataat
      361 gtggcagaac tttaaaacta gcaattcggc atattcaaaa ctatgtggaa aattatcttt
      421 atttaaaagc gtgcccagtc ggttaataga aaaatcagct tactgctcaa ctgaaaacat
      481 ggccactaat aaatttaatg tcgtcttttc ttactgcgga gataatataa aggaatttat
      541 acttttatta ccctataata aatcactgga gatgtatg
//
LOCUS       DAZPLM010000500          563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPLM010000500 DAZPLM010000000
VERSION     DAZPLM010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.503"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>563)
                     /locus_tag="ACHVC7_004571"
     CDS             complement(<1..>563)
                     /locus_tag="ACHVC7_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR2118396.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEEW"
BASE COUNT          146 a          169 c          128 g          120 t
ORIGIN      
        1 ccattcttct tccgagagct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa
       61 gcggagcaaa tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa
      121 acctccggca tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc
      181 agacaacgca attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt
      241 cgtctctaaa aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg
      301 atcgttcaga tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt
      361 gatatcgact ccctcagtta gcagcgttct ttgcattaac gccccaaaag gatcatcacc
      421 gacccgacct ataaatccac ttattccgcc taatctggcg attcccaccg caacgttagc
      481 tggcgcgccg ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac
      541 cgcatcccct aaaacccata ctt
//
LOCUS       DAZPLM010000501          560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPLM010000501 DAZPLM010000000
VERSION     DAZPLM010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.504"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..529
                     /locus_tag="ACHVC7_004572"
     CDS             <1..529
                     /locus_tag="ACHVC7_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR2118397.1"
                     /translation="LVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTV
                     NLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLK
                     NTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPGKDAT
                     PGNVYAQADYKIAYE"
BASE COUNT          162 a          127 c          131 g          140 t
ORIGIN      
        1 tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg aaggtcacgg
       61 cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac caggtgacga
      121 agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg accagaaatc
      181 tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg gtggaaccac
      241 ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc agactgacct
      301 gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct
      361 ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat tcaacaatac
      421 taacagctat caggaactga acttcaaagc tcgtatggaa tccccgggca aagatgctac
      481 cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat cacattaggc
      541 cgggttcgcc cggccacatc
//
LOCUS       DAZPLM010000502          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPLM010000502 DAZPLM010000000
VERSION     DAZPLM010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.505"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            78..557
                     /locus_tag="ACHVC7_004573"
                     /pseudo
     CDS             78..557
                     /locus_tag="ACHVC7_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          177 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagcatt
//
LOCUS       DAZPLM010000503          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPLM010000503 DAZPLM010000000
VERSION     DAZPLM010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.506"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHVC7_004574"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHVC7_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR2118398.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZPLM010000504          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPLM010000504 DAZPLM010000000
VERSION     DAZPLM010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.507"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHVC7_004575"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHVC7_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPLM010000505          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPLM010000505 DAZPLM010000000
VERSION     DAZPLM010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.508"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHVC7_004576"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHVC7_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZPLM010000506          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPLM010000506 DAZPLM010000000
VERSION     DAZPLM010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.509"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..420
                     /gene="espX1"
                     /locus_tag="ACHVC7_004577"
     CDS             <1..420
                     /gene="espX1"
                     /locus_tag="ACHVC7_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR2118399.1"
                     /translation="CFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDI
                     MPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYK
                     ELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQD"
BASE COUNT          212 a           98 c           86 g          150 t
ORIGIN      
        1 tgttttgaaa gcgacttatc aaacaccatc ttttcaaatg gagaaataaa caacctcttc
       61 attaaaaaaa gcaacatatt tggaacatcg ttcactaata ccatgataaa aaatttgcgc
      121 tgtgaagata ttatgcctgg ccgatggacc actcaacttg tcaataagca cttaggttac
      181 agatacacag gagtattcaa aaccctggca agtattgatg acaaaccgtc ccgtttcgaa
      241 attttgatac cattggtaca aactttagtt cgcgataatg taaagctaaa taatgatgtg
      301 tataaagaac ttaataagtt tatgcacgat tacgacaaaa catcatcaaa aatgagaaaa
      361 taccttcaga gcatcaacga gtgcatgctc cttataaaaa atatagttca tcaggattaa
      421 aacactcaaa gaaagaggta attttagaat gaaattatct gtgatgatta ttaatacact
      481 gcagagaaat aaaaaacaat ctgaagcatg ctctgaaaca tcctactctg tcactctaat
      541 gatggc
//
LOCUS       DAZPLM010000507          527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPLM010000507 DAZPLM010000000
VERSION     DAZPLM010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.510"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHVC7_004578"
     CDS             <1..275
                     /locus_tag="ACHVC7_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFR2118400.1"
                     /translation="KMAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            complement(254..>527)
                     /locus_tag="ACHVC7_004579"
     CDS             complement(254..>527)
                     /locus_tag="ACHVC7_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR2118401.1"
                     /translation="RASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHGWKP
                     NYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
BASE COUNT          141 a          131 c          121 g          134 t
ORIGIN      
        1 aaaaaatggc gcaggtgata gccaaattgt taccgcttca gctactgcgt ggcgttggcc
       61 tggagcaacg gcaacatgtc catctggcaa gaaagtcatt ggtggaggcg gtcaatgcag
      121 atcaaatacc ggatttatat ggctcacgcg ttccatgcct tcaggcaaca atgcatggac
      181 tgccagttgc gacacgaccg aagatcaaaa tggttcaatt acagtttacg ccatttgcca
      241 gtgacgatac ttatcactgg caaaccacga tgtaactaca tttccccgac aggtcagttc
      301 ggtgagtacc gccataaaag ttgtattcgt catagaagcc gcatttcaca attcgattgg
      361 catcataggt tgcaactgac cccgcaaaat aattcggctt ccacccgtgc ggattttctg
      421 cattactgac actccaggta cactggctgg cagaaaaacc agatggcaat gggatctgtt
      481 gtccatttgc tatttttcct gtcagaacag tgctacctga tgccctt
//
LOCUS       DAZPLM010000508          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPLM010000508 DAZPLM010000000
VERSION     DAZPLM010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.511"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>523)
                     /locus_tag="ACHVC7_004580"
     CDS             complement(<1..>523)
                     /locus_tag="ACHVC7_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118402.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTAA"
BASE COUNT          143 a          141 c          134 g          105 t
ORIGIN      
        1 gcggcggtca tattaataat agtattcatc agatcctgca cggtattgga accgagcatc
       61 gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg cgccggaacg
      121 ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat ttcggagaag
      181 aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc ggtaaaggac
      241 accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc cgccatgccg
      301 tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac gaagacgtta
      361 atcagcaccg aggagacgga atacagtacg ccgataaaaa ttccggcgag gatgatcact
      421 ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac ggactccgcg
      481 ccacctgcgg ccataaagat ccaggtggta acgccgagga acg
//
LOCUS       DAZPLM010000509          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPLM010000509 DAZPLM010000000
VERSION     DAZPLM010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.512"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..158
                     /locus_tag="ACHVC7_004581"
     CDS             <1..158
                     /locus_tag="ACHVC7_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118403.1"
                     /translation="FLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTYD
                     YDLLNAI"
BASE COUNT          170 a          115 c           94 g          141 t
ORIGIN      
        1 aattcctttt tgataatagc ctcgagaaag acgaaaattc ttcggacaat tgctttgagg
       61 tcgaaaaata taacgcatat gaagcatata actcagagaa tctatatttt accagagagg
      121 aatatacgta tgattacgac cttttaaatg caatatagac ataataactt cttacaaaat
      181 ctataattaa taccgtagat attcattcat cttaccctgt gcaccaaaag caaaaactca
      241 ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag
      301 gcctacgcca tctctacaat atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt
      361 cacgcagcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc ggaacggttt
      421 attacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agtgtcgtgg
      481 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
//
LOCUS       DAZPLM010000510          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPLM010000510 DAZPLM010000000
VERSION     DAZPLM010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.513"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..501
                     /locus_tag="ACHVC7_004582"
     CDS             <1..501
                     /locus_tag="ACHVC7_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR2118404.1"
                     /translation="ISDPNEPKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGT
                     PCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQH
                     NDSNNETGEDELNWKRQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSD
                     ERLPDF"
BASE COUNT          171 a          104 c          133 g          101 t
ORIGIN      
        1 attagcgacc cgaatgaacc gaaatggatt cccggtcatc atatctcctc aaatgaagtc
       61 aaaaaaacgg aaaatgaagt gcgacagact gaagataaac agcaccagaa cagcgaaccg
      121 gaggagggaa caccctgcaa tcagactggc gaggataact gccctgactg tggcgcggtg
      181 atgggagacg caacttatca ggaaactttt gacgaaaaaa aaccagatga agcacaggaa
      241 gaagagccga agaaaacgga aaaagctgat gatttgttac cggagaacgc tggcagcgat
      301 cagcataatg atagcaataa tgaaactggc gaagatgaac tcaactggaa aagacaggtg
      361 ttgattgcgg cggtttacgg tttatgtgcg aatcctgcgt gcatagccta tgcgcctgca
      421 atccctgaca tcgcaatgat gattgccaac aagcttgaaa attttggagt gaccagtgat
      481 gagcgcttac ccgatttttg atcgtattg
//
LOCUS       DAZPLM010000511          508 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPLM010000511 DAZPLM010000000
VERSION     DAZPLM010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.514"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHVC7_004583"
     CDS             complement(<1..494)
                     /locus_tag="ACHVC7_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118405.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          138 a          124 c          102 g          144 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtg
//
LOCUS       DAZPLM010000512          505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPLM010000512 DAZPLM010000000
VERSION     DAZPLM010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.515"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..258
                     /locus_tag="ACHVC7_004584"
     CDS             <1..258
                     /locus_tag="ACHVC7_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR2118406.1"
                     /translation="EAAWSYRHPARISPAIQKRQENLPRPVIDRAWDAQLRLCKRYRK
                     LQAKGKNVNITIVAVARELAGFIWDMGRIAMSVAQQPQCHK"
BASE COUNT          155 a          117 c          126 g          107 t
ORIGIN      
        1 gaggcagcgt ggagttaccg tcatccggca cgcatcagtc cggcaataca aaaaaggcag
       61 gaaaatttac cccgccccgt cattgacaga gcatgggatg ctcaactcag gctttgtaag
      121 aggtatcgaa aacttcaggc caaaggaaag aatgtcaata ttacaattgt tgctgttgca
      181 cgtgagctgg cgggttttat ctgggatatg ggccgaatag caatgtctgt cgcacaacaa
      241 ccacaatgtc acaaataaaa aatgagaata accagttccc tgaaggcgac gtagcccggt
      301 acaagagtaa cccacgttag cgcttggcga cggcggaaca aagctgattc gcgacgtaag
      361 aaagtggcag gctcatatga cggacctctg taatgcggta tccaacccgc gaatatcagc
      421 gtgattcacc gtcggattta ctgctacgtc gccctcaggg aattaaacct aaaaagaaaa
      481 tcgtcaaaat tagagattcc ggttt
//
LOCUS       DAZPLM010000513          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPLM010000513 DAZPLM010000000
VERSION     DAZPLM010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.516"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(105..413)
                     /locus_tag="ACHVC7_004585"
                     /pseudo
     CDS             complement(105..413)
                     /locus_tag="ACHVC7_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
BASE COUNT          138 a          129 c          120 g          116 t
ORIGIN      
        1 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
       61 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cggaggtgat gctgccaact
      421 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
      481 tgtccctcct gttcagctac tga
//
LOCUS       DAZPLM010000514          499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPLM010000514 DAZPLM010000000
VERSION     DAZPLM010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.517"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(182..373)
                     /locus_tag="ACHVC7_004586"
     CDS             complement(182..373)
                     /locus_tag="ACHVC7_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR2118407.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            391..>499
                     /locus_tag="ACHVC7_004587"
     CDS             391..>499
                     /locus_tag="ACHVC7_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118408.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVME"
BASE COUNT          160 a           91 c          116 g          132 t
ORIGIN      
        1 tatcattaca acatatatat taattttata tttattccgc agccaaacag gataaacatc
       61 agtcaggaca aagacgtaaa atttaagatg aaagaatatc tgccgctttg ataatcccaa
      121 agagcaaaag catccgacag cgaagtgccg ttatgttctg tgaaagtgaa gaaggtaacc
      181 gctaacgttg taccggtgat tcgacggaga gtgtggattg ctgagcttct tttttgctct
      241 gatggtgctg ccaggcaccg acggacgagt agataaaacg gccaaagaag aagataaagc
      301 tgatgagcag tacgatacgg gtcatgcgac tgttaaatcg gtgtcgtttt cgcatactgg
      361 ttgcctgact cacaaaaggt tccttgaagt atgtcccacg ctgtggacgg tacttacatt
      421 aaggcacaac aggacaaaat ggtcaattct tcgttatgta aaaaaacgtc agtggataca
      481 tattttaatg ttatggaag
//
LOCUS       DAZPLM010000515          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPLM010000515 DAZPLM010000000
VERSION     DAZPLM010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.518"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            69..473
                     /gene="stpA"
                     /locus_tag="ACHVC7_004588"
     CDS             69..473
                     /gene="stpA"
                     /locus_tag="ACHVC7_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR2118409.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          145 a          107 c          122 g          120 t
ORIGIN      
        1 aataagtgat cgcttacact acgcgacgaa atactttttt gttttggcgt taaaaggttt
       61 tctttattat gtccgtaatg ttacaaagtt taaataacat tcgcaccctc cgtgcgatgg
      121 ctcgcgaatt ctccattgac gttcttgaag aaatgctcga aaaattcagg gttgtcacta
      181 aagaaagacg tgaagaagaa gaacagcagc agcgtgaact ggctgagcgt caggaaaaaa
      241 ttagcacctg gctggagctg atgaaggctg acggaattaa cccggaagag ttattgggta
      301 atagctctgc tgctgcacca cgcgctggta aaaaacgcca gccgcgtccg gcgaaatata
      361 aattcaccga tgttaacggt gaaactaaaa cctggaccgg tcagggccgt acaccgaagc
      421 caattgctca ggcgctggca gaaggtaaat ctctcgacga tttcctgatc taattactga
      481 ggccggatgt cgct
//
LOCUS       DAZPLM010000516          491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPLM010000516 DAZPLM010000000
VERSION     DAZPLM010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.519"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>491
                     /locus_tag="ACHVC7_004589"
     CDS             <1..>491
                     /locus_tag="ACHVC7_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2118410.1"
                     /translation="NTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLVQTATRF
                     SVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNV
                     SLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIPF
                     YWG"
BASE COUNT          152 a           98 c           98 g          143 t
ORIGIN      
        1 aatacaccgc tgggtgccat ctctttttac gctgcccgat cgagtagtaa gttaaataat
       61 gataccagac acgaaggtac gagctatcag gtcgcctata ataaatatct agtacaaaca
      121 gccactcgat ttagcgtggc cgcctggcgt tatgcttcgc aggattacag gacattcagc
      181 gaccatcttt acgaaaatga taaacactat catcagagtg attatgatga tttttatgat
      241 attggcagaa aaaatagcct ttctgccaat attatgcaac ctttatccaa taatctggga
      301 aatgtatcat taagtgcgct ttggcggaat tactgggggc gaagtggaaa tgctaaagat
      361 taccaattca gttactccaa taactggcaa cacattagtt atactttctc tgccagccaa
      421 tcttatgatg aaaataataa agaagaggag cgttttaatc tgtttatctc cattcctttc
      481 tactgggggg a
//
LOCUS       DAZPLM010000517          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPLM010000517 DAZPLM010000000
VERSION     DAZPLM010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.520"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHVC7_004590"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHVC7_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR2118411.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          111 a          136 c          134 g          109 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc
//
LOCUS       DAZPLM010000518          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPLM010000518 DAZPLM010000000
VERSION     DAZPLM010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.521"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHVC7_004591"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHVC7_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR2118412.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPLM010000519          488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPLM010000519 DAZPLM010000000
VERSION     DAZPLM010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.522"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>488
                     /locus_tag="ACHVC7_004592"
     CDS             <1..>488
                     /locus_tag="ACHVC7_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFR2118413.1"
                     /translation="PKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRG
                     PMETGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNV
                     DRVALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRA
                     AI"
BASE COUNT          146 a          100 c           72 g          170 t
ORIGIN      
        1 accaaaaaaa acaataacta acaatcctac tttgttaatt ttcatctcat tatgtctttt
       61 gggaatcata aatatcatct ggtattcaca ttataaagtt tcaggctctg tttataccaa
      121 tgcatatcgt ggcccaatgg aaactgggaa aattgccttg tgtagtgctt ttattttctt
      181 ggttcttttt gctaaaaatg agttgagaac aaaaataaaa tttgggaaac taattctgtt
      241 cgcatccctg gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac atttctattt
      301 aaacgttgac cgtgttgcat tatcagcttc ccacgctaca acagcaggct acatcatcct
      361 tttcccttct ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac
      421 attatataca attaacttca tgcttagctt atgtgctgtc atagtaactg aaacgcgtgc
      481 agccatat
//
LOCUS       DAZPLM010000520          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPLM010000520 DAZPLM010000000
VERSION     DAZPLM010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.523"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            30..>480
                     /locus_tag="ACHVC7_004593"
     CDS             30..>480
                     /locus_tag="ACHVC7_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR2118414.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          117 a          116 c          123 g          124 t
ORIGIN      
        1 accccggtat ttccaccacg gaggccagca tgaacaacaa taacacattg tacgtcgggt
       61 tagatgttca caaagaatcg attactgtcg cttatgctat caattcagaa cctgttgaac
      121 tgatgggtaa aattggcaca tcacctactg atattcagaa tctttgtaaa cgtctcaggt
      181 caaagtcatc gcaggtcagt atcgtttatg aagcggggcc ctgtggttat ggactttatc
      241 gtcggctggt gaaatccggt tttgactgca tggtctgcgc cccctcactt atcccgaaaa
      301 aaccggggga gcgggttaaa accgatcgcc gtgatgccat cagacttgtg cgttcactgc
      361 gtgcaggaga tctttctgct gtctacgtac ccggcattga agatgaggca ttccgggatt
      421 tggcccgggc atgggcatct gcccgcgatg atttacggca tgcaaggcaa cgtctgaaat
//
LOCUS       DAZPLM010000521          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPLM010000521 DAZPLM010000000
VERSION     DAZPLM010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.524"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>480
                     /locus_tag="ACHVC7_004594"
     CDS             <1..>480
                     /locus_tag="ACHVC7_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2118415.1"
                     /translation="PLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLD
                     QENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASM
                     VGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQ"
BASE COUNT          139 a          118 c          123 g          100 t
ORIGIN      
        1 agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc ttccagaatc
       61 gggctgcttt tccccgattc aaaaagcggg gacagaatga tgtattccgc tacccgcagg
      121 gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc tggatgcgct
      181 accggaacag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg
      241 gtaagtggta catcagtatt cagacagaaa gtgaagtatc aactccggtt cacccttcag
      301 catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag
      361 tctttgagcc tgtaaacagt tttcagaaaa accagaagaa gctggcgaga cttcagcgac
      421 agttaagccg caaggtcaaa ttcagcaaca actggcagaa gcagaaacgc aaaatacagc
//
LOCUS       DAZPLM010000522          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPLM010000522 DAZPLM010000000
VERSION     DAZPLM010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.525"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHVC7_004595"
     rRNA            <1..>476
                     /locus_tag="ACHVC7_004595"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPLM010000523          470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPLM010000523 DAZPLM010000000
VERSION     DAZPLM010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.526"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHVC7_004596"
     CDS             <1..358
                     /locus_tag="ACHVC7_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2118416.1"
                     /translation="LFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYV
                     IQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFA
                     EQEILSEFPQPVSLAG"
     gene            complement(401..>470)
                     /locus_tag="ACHVC7_004597"
     CDS             complement(401..>470)
                     /locus_tag="ACHVC7_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118417.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          130 a          100 c           92 g          148 t
ORIGIN      
        1 tcttttctat ctgaataata attatcgcga aaaaatcact ttagaacagt tatcgaaaaa
       61 atttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc gtatttcccc
      121 tattaactat gttattcaac ggcgtatgac ggaagcaaaa tggtcactca ctaatactga
      181 attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc actttgccaa
      241 actgtttttg cgccatgtcg gttgttcgcc cagcgattac cgtaggcaat ttaaaaattg
      301 ttttgcggaa caagaaatct tatctgaatt tcctcaaccg gtaagtcttg ccggataaat
      361 ctggcggcgc actttgttaa aaagctgcgc cgcctgcaga ttatttgttc tgatagtctt
      421 tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
//
LOCUS       DAZPLM010000524          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPLM010000524 DAZPLM010000000
VERSION     DAZPLM010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.527"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHVC7_004598"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHVC7_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          113 a          110 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa cccctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPLM010000525          466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPLM010000525 DAZPLM010000000
VERSION     DAZPLM010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.528"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pagP"
                     /locus_tag="ACHVC7_004599"
     CDS             <1..465
                     /gene="pagP"
                     /locus_tag="ACHVC7_004599"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR2118418.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          119 a          107 c          122 g          118 t
ORIGIN      
        1 acgtttagag aaaatattgc acaaacctgg caacagcctg aacattatga tttatatatt
       61 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
      121 gagcgaccgt ggggtggcgg ttttggtcag tcgcgatggg atgaaaaagg aaactggcat
      181 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
      241 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
      301 accgctggcg taacggcacg cgataactgg aattacatcc ctctcccggt tctactgcca
      361 ttggcctccg tgggttatgg cccagtgact tttcagatga cctacattcc gggtacctac
      421 aacaatggca atgtgtactt tgcctggatg cgctttcagt tttgag
//
LOCUS       DAZPLM010000526          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPLM010000526 DAZPLM010000000
VERSION     DAZPLM010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.529"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>459
                     /locus_tag="ACHVC7_004600"
     CDS             <1..>459
                     /locus_tag="ACHVC7_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2118419.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHS"
BASE COUNT          118 a          106 c          148 g           87 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagt
//
LOCUS       DAZPLM010000527          457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPLM010000527 DAZPLM010000000
VERSION     DAZPLM010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.530"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHVC7_004601"
     CDS             <1..118
                     /locus_tag="ACHVC7_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR2118420.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNYEKLQSS"
     gene            225..>457
                     /locus_tag="ACHVC7_004602"
     CDS             225..>457
                     /locus_tag="ACHVC7_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR2118421.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          162 a           93 c           98 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattacgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAZPLM010000528          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPLM010000528 DAZPLM010000000
VERSION     DAZPLM010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.531"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHVC7_004603"
     CDS             <1..386
                     /locus_tag="ACHVC7_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2118422.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          105 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcact
//
LOCUS       DAZPLM010000529          453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPLM010000529 DAZPLM010000000
VERSION     DAZPLM010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.532"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHVC7_004604"
     CDS             complement(<1..396)
                     /locus_tag="ACHVC7_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2118423.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          119 a          124 c           77 g          133 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atc
//
LOCUS       DAZPLM010000530          447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPLM010000530 DAZPLM010000000
VERSION     DAZPLM010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.533"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHVC7_004605"
     tRNA            54..130
                     /locus_tag="ACHVC7_004605"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHVC7_004606"
     tRNA            173..248
                     /locus_tag="ACHVC7_004606"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          127 a           93 c          108 g          119 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagc
//
LOCUS       DAZPLM010000531          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPLM010000531 DAZPLM010000000
VERSION     DAZPLM010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.534"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHVC7_004607"
     CDS             <1..>442
                     /locus_tag="ACHVC7_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2118424.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZPLM010000532          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPLM010000532 DAZPLM010000000
VERSION     DAZPLM010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.535"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHVC7_004608"
     rRNA            <1..>442
                     /locus_tag="ACHVC7_004608"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          118 a           98 c          131 g           95 t
ORIGIN      
        1 gagaaggcac gctgatatgt aggtgaagtc ccttgcggat ggagctgaaa tcagtcgaag
       61 ataccagctg gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac
      121 gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt gatggggtca gcgcaagcga
      181 agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga
      241 aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc
      301 cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg
      361 gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg
      421 ataggtggga ggctttgaag tg
//
LOCUS       DAZPLM010000533          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPLM010000533 DAZPLM010000000
VERSION     DAZPLM010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.536"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
BASE COUNT          156 a           45 c           77 g          160 t
ORIGIN      
        1 ataatttatt ggttgtatgt taaatctatc ataagtctaa tgattaaaat ggttagtggt
       61 gtcaggatta gtgaaaataa aggatgtaat atgctattct ttctctgtaa gtacttgaga
      121 atactaaacc atagtacaag aacgattatg tacatgtagt gagaattgga aaacatagtt
      181 tcttcccgtg ttaatgagag tatgtgtttt tatgtgggga tatgtacacc ccatatagat
      241 gtattatgtt atatttataa tatacctcgg caaaataaaa ggattgttat agaaataggg
      301 aagctataaa aaagcttata ttaaggtctg ttttcttctt agaaaaataa ttctttgttc
      361 taattattat agatgtaatc ccccaaacaa taaaaagaat ggagctgctt gtaattattt
      421 gatatatcaa aattgttt
//
LOCUS       DAZPLM010000534          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPLM010000534 DAZPLM010000000
VERSION     DAZPLM010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.537"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHVC7_004609"
     CDS             <1..>436
                     /locus_tag="ACHVC7_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2118425.1"
                     /translation="WHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKAL
                     AEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGV
                     CGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          121 t
ORIGIN      
        1 tggcatattg ctgccaatat tcagtttcca ccaaattttg atgagtcaaa acagtatccg
       61 gcgattatca gcgtccatcc ttttggcagt tgtaaggagc agacgtctgg caatatctat
      121 ggcaaggcat tggctgaaaa aggctatctc gttctcgctt atgacgccag tttccaggga
      181 gaatctggtg gggagcctcg ctggatagaa gatccgacac agcgcgtaga ggatattagc
      241 cgtgttatcg attatgcagt aactctgcca tatgttaatg ctgaacgtat tggcgtgctg
      301 ggagtgtgtg ggggtggtgg ctatgccatt aatgccgcgc tgacagaaaa acgaattaaa
      361 gcagtagtga gtattacggg tgttaatatc gggcgtcttt ttcgggaagg tttcagcaat
      421 tacgatccga ttggcg
//
LOCUS       DAZPLM010000535          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPLM010000535 DAZPLM010000000
VERSION     DAZPLM010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.538"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHVC7_004610"
     CDS             complement(<1..112)
                     /locus_tag="ACHVC7_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118426.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>432)
                     /locus_tag="ACHVC7_004611"
     CDS             complement(368..>432)
                     /locus_tag="ACHVC7_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR2118427.1"
                     /translation="INVNFFSSDRTSFCECNRFP"
BASE COUNT          125 a           89 c           86 g          132 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat ca
//
LOCUS       DAZPLM010000536          428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPLM010000536 DAZPLM010000000
VERSION     DAZPLM010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.539"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(141..353)
                     /gene="cspH"
                     /locus_tag="ACHVC7_004612"
     CDS             complement(141..353)
                     /gene="cspH"
                     /locus_tag="ACHVC7_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR2118428.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          129 a           83 c           91 g          125 t
ORIGIN      
        1 catttataac tattaggtgc agagatattc gcttaaatgg attagtttct ttgtggaaaa
       61 gtaactgatg ttataatgta tgatgttgac tattgcacta ttccaataaa accagtcagc
      121 tttaaacaag cagcgtcata ttaagagaga taaacatttg ccgctgttgg tcctcgcagg
      181 ccatttacgc ggcaaaattc cacacgtaat cctggtataa gcacttctgc gtcgcgggga
      241 gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt cggaggggat aatgaatcct
      301 ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca ttttacggga caaacaaatt
      361 ccttactgaa aatactgcgc tgcactatac ggggttaata aaataaagcc agcgatattt
      421 aagaccgc
//
LOCUS       DAZPLM010000537          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPLM010000537 DAZPLM010000000
VERSION     DAZPLM010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.540"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHVC7_004613"
     CDS             <1..411
                     /locus_tag="ACHVC7_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2118429.1"
                     /translation="AERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPA
                     LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGV
                     EPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          108 a          113 c          126 g           80 t
ORIGIN      
        1 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
       61 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata tgccctgaac
      121 cagtggccgg cgctgacgta ctatgcagat gatggctggg ctgaggcgga caataacatc
      181 gctgaaaatg cgttgcggat ggtcagtctg ggccgcaaaa actacctgtt cttcggttcg
      241 gatcatggag gagagcgggg agcgctgctg tacagcctga tcgggacgtg caaactgaac
      301 ggagtggagc cagaaagcta cctccgctat gtccttgacg tcatagccga ctggccgata
      361 aaccgggtcg gcgaactgct cccctggcgc gtagcactgc cgactgaata acacattccc
      421 gtcaata
//
LOCUS       DAZPLM010000538          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPLM010000538 DAZPLM010000000
VERSION     DAZPLM010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.541"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHVC7_004614"
     tRNA            41..116
                     /locus_tag="ACHVC7_004614"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPLM010000539          417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPLM010000539 DAZPLM010000000
VERSION     DAZPLM010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.542"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>417
                     /locus_tag="ACHVC7_004615"
     CDS             <1..>417
                     /locus_tag="ACHVC7_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2118430.1"
                     /translation="MLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLG
                     GTPLVEYTRDRLHRETARSFGGEAYELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQ
                     LIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIP"
BASE COUNT           88 a          123 c          149 g           57 t
ORIGIN      
        1 gatgctgtgg gagcatgaga cggggcacgc gtacagcgaa caggggctgg cgacccgtca
       61 ggagccggac ggcctgccgc cggtggagtg gctgacgtat ggcagcggtt atcttgcggg
      121 gatgaagctg ggcggaacac cgctggtcga gtacacgcgg gaccggctgc accgtgagac
      181 ggcccgcagc ttcggcgggg aggcatatga acttgccacc gcctggaata ccagcggcca
      241 gctccggagc aggcacctga accttccgca gcttgaccgt gactacgact ggaacgacaa
      301 cggacagcta atccgcatca gtggcccgca ggagagccgg gagtaccgtt acagcgacac
      361 ggggaggctg acgggcgtcc acaccacggc agcgaacctg gatatcgata tcccgta
//
LOCUS       DAZPLM010000540          411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPLM010000540 DAZPLM010000000
VERSION     DAZPLM010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.543"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>411
                     /locus_tag="ACHVC7_004616"
     CDS             <1..>411
                     /locus_tag="ACHVC7_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR2118431.1"
                     /translation="IFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAAVIRWYAERD
                     AEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETA
                     FCTFVLSRIAGEIASILDGIPLSVQRRFPELENRH"
BASE COUNT          102 a           98 c          128 g           83 t
ORIGIN      
        1 attttcggtg cgagtatccg taccattcag aactggcagg aacagggaat gcccgttctg
       61 cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg ccgccgttat aagatggtat
      121 gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgcgg
      181 caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg
      241 cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga agtggtggaa
      301 accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac
      361 gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca t
//
LOCUS       DAZPLM010000541          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPLM010000541 DAZPLM010000000
VERSION     DAZPLM010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.544"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..248
                     /locus_tag="ACHVC7_004617"
                     /pseudo
     CDS             1..248
                     /locus_tag="ACHVC7_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          169 a           66 c           57 g          117 t
ORIGIN      
        1 agcaaaagcg gaaattgctt cgacaataat taaattttta gaggccagga taaccaatct
       61 ttcatacaat gacatattaa aatatcagca agaattccaa aaacaatgtt ataagcaagt
      121 taaagccttt acgacactct ctcgatacaa taaaattcaa acatgggcag aaatgtctga
      181 atatcagttc gaagtatttc aatacgagac gcttaacccc aaaaaaatgt cacacacgcc
      241 ttatttaaag aggccacttc ctaatgaaaa agatataaat tatggggtgg aaatagaaat
      301 accatctggc aaaagaattc gcttatcaaa tcattatcag aatataatac cataatattg
      361 attattagca ctttataatt attgaatgaa aatagatttt atgtaattt
//
LOCUS       DAZPLM010000542          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPLM010000542 DAZPLM010000000
VERSION     DAZPLM010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.545"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..408
                     /locus_tag="ACHVC7_004618"
                     /pseudo
     CDS             1..408
                     /locus_tag="ACHVC7_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
BASE COUNT          159 a           66 c           55 g          128 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtt
//
LOCUS       DAZPLM010000543          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPLM010000543 DAZPLM010000000
VERSION     DAZPLM010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.546"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>398
                     /locus_tag="ACHVC7_004619"
     CDS             <1..>398
                     /locus_tag="ACHVC7_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR2118432.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          105 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatga
//
LOCUS       DAZPLM010000544          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPLM010000544 DAZPLM010000000
VERSION     DAZPLM010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.547"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>391)
                     /locus_tag="ACHVC7_004620"
     CDS             complement(<1..>391)
                     /locus_tag="ACHVC7_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2118433.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          135 c          113 g           72 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcattttc c
//
LOCUS       DAZPLM010000545          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPLM010000545 DAZPLM010000000
VERSION     DAZPLM010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.548"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            77..>390
                     /locus_tag="ACHVC7_004621"
     CDS             77..>390
                     /locus_tag="ACHVC7_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118434.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNT"
BASE COUNT          145 a           61 c           60 g          124 t
ORIGIN      
        1 gtatcatcgt aaatatgtct ttcagacatg tattgtttat tttgttacta acaagtaaaa
       61 atattacagg ttatttatga aagtatttca caatatattt aagcacttct cttcaaacca
      121 tcaagataag cattcagata aagttaacag tcatcaacac catggcaagg ttgataaaaa
      181 atatcgatca aacacagttg aagttaatat gctgaatgac ttttcgcaaa tagataacga
      241 tttaggcctg aatatcatct atttcctgca acatggtcac tggagggtaa atgaacatag
      301 tcttcagatg gagaaaatat ggttttacaa tagtgagcca tctatagata ttcaagaata
      361 taatagtttc gccgataata ctactaacac
//
LOCUS       DAZPLM010000546          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPLM010000546 DAZPLM010000000
VERSION     DAZPLM010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.549"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..65)
                     /locus_tag="ACHVC7_004622"
     CDS             complement(<1..65)
                     /locus_tag="ACHVC7_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR2118435.1"
                     /translation="MFDGELSFALKLAREMGRPDWR"
     gene            complement(47..>380)
                     /locus_tag="ACHVC7_004623"
     CDS             complement(47..>380)
                     /locus_tag="ACHVC7_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR2118436.1"
                     /translation="LMKRQAEQAESDSNRKFTVEDAIRTGAFVVAMSLWHNHPKKTQM
                     PSMNEAVKQIEQEVLTTWPTEAISHAENVVYRLSGMYEFVVNDAPEQAEDAGPAEPVS
                     AGKCSTVS"
BASE COUNT           78 a          122 c           89 g           91 t
ORIGIN      
        1 cgccagtcgg gtcgccccat ctcacgcgcc agtttcaggg caaaactcag ctcaccgtcg
       61 aacactttcc cgcagaaaca ggctctgcag gcccggcgtc ctctgcctgt tcaggagcat
      121 cattcaccac aaactcatac ataccggaca gccggtacac cacgttttca gcatgagaaa
      181 ttgcctctgt gggccaggtg gtaagcactt cctgctcaat ctgtttaacg gcttcattca
      241 tggacggcat ctgcgtcttc ttcggatggt tatgccacag ggacatcgca accacaaaag
      301 cgccggttct gatggcgtct tccacagtaa acttccggtt gctgtctgac tccgcctgtt
      361 ctgcctgccg tttcatcagg
//
LOCUS       DAZPLM010000547          377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPLM010000547 DAZPLM010000000
VERSION     DAZPLM010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.550"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>377
                     /locus_tag="ACHVC7_004624"
     CDS             <1..>377
                     /locus_tag="ACHVC7_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2118437.1"
                     /translation="IEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYG
                     KKHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTF
                     LGDKRIEGSVWPKSIRGSTPKVRG"
BASE COUNT           92 a           91 c          110 g           84 t
ORIGIN      
        1 atagagcata agcagcgcaa cacacttatc tggttgccga cggatggtga tgccgagaac
       61 tttatgaaaa cccacgttga gccgaccatc cgcgatattc cgtcgctgct ggcgctggct
      121 ccgtggtatg gcaaaaagca ccgggataac acgctcacta tgaagcgttt ttccaatggt
      181 cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat
      241 gtggcgggtt atgatgaact tgctgccttt gatgaggata ttgaacagga aggctctccg
      301 acgttccttg gcgacaaacg tattgaaggc tcggtctggc caaagtccat ccgtggctcc
      361 acgcccaaag tgagagg
//
LOCUS       DAZPLM010000548          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPLM010000548 DAZPLM010000000
VERSION     DAZPLM010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.551"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(212..>372)
                     /locus_tag="ACHVC7_004625"
     CDS             complement(212..>372)
                     /locus_tag="ACHVC7_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR2118438.1"
                     /translation="VKLSMPELEELAAAMVQAVVERNDEIYRRQREMKEELSSLDDLA
                     SIRAFDVK"
BASE COUNT           87 a          128 c           74 g           83 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttctctgttc
      121 agcctgaccg cacaccccgg cggccggatg acagactccg cctcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attacttaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagactgctc agctcctctt tcatttcacg ctgacggcga taaatctcgt
      301 cattgcgttc aacaacagcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ct
//
LOCUS       DAZPLM010000549          364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPLM010000549 DAZPLM010000000
VERSION     DAZPLM010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.552"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(63..>364)
                     /locus_tag="ACHVC7_004626"
     CDS             complement(63..>364)
                     /locus_tag="ACHVC7_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118439.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          121 a           62 c           66 g          115 t
ORIGIN      
        1 agacgttata aaagacaaat agattgtgat cgcctacaga cgatcacaat gaaaggaaaa
       61 tattaacggt ttttgctatc actcaaatgt ttttcatcct gcaccatatc attgccagat
      121 tcatgcaaag tatttattaa tgcattggca tctggatatt gttgctgata cttggttaga
      181 tatatatcac tatggatatg ttcggcaatt atattgataa gcaagcgatt attcgagtat
      241 aaccccagtg attcagctcg ttgtgaaaaa accattactg tcttcatatc aacaacaagc
      301 ataaaacggt gagaagggta atattcttct tcatggttaa agcggtaata cagttctaca
      361 cgtg
//
LOCUS       DAZPLM010000550          364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPLM010000550 DAZPLM010000000
VERSION     DAZPLM010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.553"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHVC7_004627"
     CDS             <1..347
                     /locus_tag="ACHVC7_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR2118440.1"
                     /translation="GTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCY
                     GFGNGRFGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELG
                     KAIGQSIKNHLE"
BASE COUNT          111 a           55 c           93 g          105 t
ORIGIN      
        1 gtggtacatg gaaggactgg ccgaaagagt tggttgccgc ttgtcatatg aaatccagcg
       61 gtaaagggta tgctagtgta tatgggcgca tgtcttggga taagccaagt ccaacaatta
      121 caactctttg ttatggtttc gggaatgggc gttttggaca tccagaacag catagagcga
      181 tttctcttag agaggctgct ttattgcaaa ctttcccgat ggattatatt tttgtagaag
      241 acaaaaacaa gtttgttata agaagtattg ggaaaatgat aggtaatgct gttcctgtgg
      301 aactaggaaa agcaattggg caatcaatca agaaccattt agaatgataa aatggcctgc
      361 ttta
//
LOCUS       DAZPLM010000551          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPLM010000551 DAZPLM010000000
VERSION     DAZPLM010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.554"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
BASE COUNT           94 a           85 c          108 g           66 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttg
//
LOCUS       DAZPLM010000552          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPLM010000552 DAZPLM010000000
VERSION     DAZPLM010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.555"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHVC7_004628"
                     /pseudo
     CDS             complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHVC7_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          117 a           64 c           61 g          107 t
ORIGIN      
        1 ttaagtgata ctggttatct ggagattcag ggggccagtc taagttctaa gagagcacgg
       61 attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca
      121 atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta
      181 aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga
      241 gatttttaaa caaagcacat tgaaaaaata aatctctctg ggattgaatt attcatgtca
      301 gcctcgtaga aatattaacc taataaaatt atgctgtgta tagtctaaa
//
LOCUS       DAZPLM010000553          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPLM010000553 DAZPLM010000000
VERSION     DAZPLM010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.556"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            57..>344
                     /locus_tag="ACHVC7_004629"
     CDS             57..>344
                     /locus_tag="ACHVC7_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2118441.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSIS"
BASE COUNT          101 a           93 c           77 g           73 t
ORIGIN      
        1 acgaattaga caaagtggtg tccaccaaat aagtagtggg aaccaaagtg tcagatatgc
       61 agaaaaatgt gactcccggc aggcgaaaag gctgccctaa ttatcctccc gaatttaaac
      121 agcagctcgt tgctgcctcc tgtgaacccg ggatatccat ctcaaaactt gctcttgaaa
      181 atggcattaa cgccaatctg ttgttcaaat ggcgacaaca atggcgcgag ggaaagctgc
      241 tattaccttc ttcagagagc ccccagctac ttcctgtgac tctcgatgca gctgccgaac
      301 agccagaatc gctcgcagag gacccgaaaa ccctcagtat cagc
//
LOCUS       DAZPLM010000554          341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPLM010000554 DAZPLM010000000
VERSION     DAZPLM010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.558"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /locus_tag="ACHVC7_004630"
     CDS             complement(<1..305)
                     /locus_tag="ACHVC7_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2118442.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          103 a           72 c           73 g           93 t
ORIGIN      
        1 cttgaatcct ctctcggcac ggttgcaatt agcgagtggg tagtatcacc aacagcgcga
       61 aatccgcgtc tgataatgga tatgttctga tataacgtgt tagtaggcac acgcatacct
      121 tcttcttccc agactctggt gaaaaaatct tgttgtgtaa ccacatcagg aaaagcttca
      181 agtagaagtg ataaacatcg actggctggc gttgtcagaa cgacgttaag atcggcgtta
      241 tgtaccgata ttaattttcg gtgctcaggc cagaactcga tattatcgtt aataatccag
      301 tacatacgtc aataaacact aaatcaatcg aaatggagat c
//
LOCUS       DAZPLM010000555          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPLM010000555 DAZPLM010000000
VERSION     DAZPLM010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.559"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>340
                     /locus_tag="ACHVC7_004631"
     CDS             <1..>340
                     /locus_tag="ACHVC7_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118443.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEG"
BASE COUNT           82 a          124 c           88 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg
//
LOCUS       DAZPLM010000556          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPLM010000556 DAZPLM010000000
VERSION     DAZPLM010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.560"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            128..334
                     /locus_tag="ACHVC7_004632"
     CDS             128..334
                     /locus_tag="ACHVC7_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR2118444.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT           96 a           71 c           82 g           86 t
ORIGIN      
        1 caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaaaggc ggtactattt
       61 tcatcagata aacaaattca tttttccggt gctattcacc gggtagatac aaaaggggga
      121 gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac gccgtacata
      181 tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg
      241 cttttacagc tacctgcacc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga
      301 tcaggccgaa aaggcaaccg gtgattttta ctgat
//
LOCUS       DAZPLM010000557          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPLM010000557 DAZPLM010000000
VERSION     DAZPLM010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.561"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..236
                     /locus_tag="ACHVC7_004633"
     CDS             <1..236
                     /locus_tag="ACHVC7_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118445.1"
                     /translation="NKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRN
                     ALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            275..>335
                     /locus_tag="ACHVC7_004634"
     CDS             275..>335
                     /locus_tag="ACHVC7_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR2118446.1"
                     /translation="MEVFMYKITATIEKEGGSPA"
BASE COUNT          116 a           70 c           78 g           71 t
ORIGIN      
        1 tcaacaagca ccatgggggc accagtaacg acgccaaacg cgcaaaagct gatttgctgg
       61 aaaaaacatt cacctcccgc tcatgggaaa gaatcaaagg tatggactgg ccgacaatca
      121 aagctggtcg taatgcacta tggattgaac tggaaggcgt tgaatatgca ttcccggatc
      181 cacaaacaca aaacgaaacg caacaagcag gatacgatga gaatattccg gtgtaaaacc
      241 aagtgcattt tggacacggt ttttgaaaat attaatggag gtgttcatgt ataaaattac
      301 agccactatt gaaaaggaag gtggttctcc tgcca
//
LOCUS       DAZPLM010000558          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZPLM010000558 DAZPLM010000000
VERSION     DAZPLM010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.562"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHVC7_004635"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHVC7_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZPLM010000559          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZPLM010000559 DAZPLM010000000
VERSION     DAZPLM010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.563"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>330
                     /locus_tag="ACHVC7_004636"
     CDS             <1..>330
                     /locus_tag="ACHVC7_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR2118447.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSS"
BASE COUNT           90 a           70 c           86 g           84 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt
//
LOCUS       DAZPLM010000560          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZPLM010000560 DAZPLM010000000
VERSION     DAZPLM010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.565"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..147
                     /locus_tag="ACHVC7_004637"
     CDS             <1..147
                     /locus_tag="ACHVC7_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR2118448.1"
                     /translation="KRVATVQKDGRRVEFTATSVSDLKKYIAELEVQTGMTQRRRGPA
                     GFYV"
     gene            144..>325
                     /locus_tag="ACHVC7_004638"
     CDS             144..>325
                     /locus_tag="ACHVC7_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2118449.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARA"
BASE COUNT           75 a           85 c          106 g           59 t
ORIGIN      
        1 aaacgggtgg caacggtaca gaaagacgga cggcgagtgg agtttacggc cacttccgtg
       61 tctgacctga aaaaatacat tgcggagctg gaggtgcaga ccggcatgac acagcgacgc
      121 aggggacctg caggatttta tgtatgaaaa cgcccaccat tcccaccctt ctgggaccgg
      181 acggcatgac atcgctgcgt gaatatgccg gttatcacgg cggtggcagc ggatttggtg
      241 ggcagttgcg ggcgtggaac ccaccgggtg aaagtgtgga tgcagccctg ctgcccaact
      301 ttacccgtgg caatgcccgc gcaga
//
LOCUS       DAZPLM010000561          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZPLM010000561 DAZPLM010000000
VERSION     DAZPLM010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.566"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>320
                     /locus_tag="ACHVC7_004639"
     CDS             <1..>320
                     /locus_tag="ACHVC7_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFR2118450.1"
                     /translation="PPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTL
                     GETWEAKIGERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEE
                     SWLI"
BASE COUNT           82 a           77 c          101 g           60 t
ORIGIN      
        1 ccacctgaca gtgtgacctt tcacatctgg acagcgtaca gcccgttcac cacctgggtg
       61 cagattgtca aagactggat gaaaacgaaa ggggatacgg gaaaacgtaa aaccttcgta
      121 aacaccacgc tcggtgagac gtgggaggcg aaaattggcg aacgtccgga tgctgaagtg
      181 atggcagagc ggaaagagca ttattcagcg cccgttcctg accgtgtggc ttacctgacc
      241 gccggtatcg actcccagct ggatcgctac gaaatgcgcg tatggggatg ggggccgggt
      301 gaggaaagct ggctgattga
//
LOCUS       DAZPLM010000562          315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZPLM010000562 DAZPLM010000000
VERSION     DAZPLM010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.567"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            49..>315
                     /gene="iraM"
                     /locus_tag="ACHVC7_004640"
     CDS             49..>315
                     /gene="iraM"
                     /locus_tag="ACHVC7_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR2118451.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKIT"
BASE COUNT          114 a           55 c           54 g           92 t
ORIGIN      
        1 gaacatccaa atgaactata ttaaataccg tgggataaga cataacaaat gaagtgggta
       61 gtaattgaca cggtaattca acctacatgc ggcatatctt tttcagccat atggggtgat
      121 atgaaaatga tcatctggta tcaatctact atatttcttc ctcctggaag tatatttaca
      181 ccggttaagc ttggtattat ccttaaggaa aaagaatatc ctattactat ttataacatt
      241 gcaccattca acaagaattt atggagttta ctcaaaagca gtcaagagtg tcctccagga
      301 gaaagcaaaa taaca
//
LOCUS       DAZPLM010000563          313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZPLM010000563 DAZPLM010000000
VERSION     DAZPLM010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.568"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(230..>313)
                     /locus_tag="ACHVC7_004641"
     CDS             complement(230..>313)
                     /locus_tag="ACHVC7_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118452.1"
                     /translation="AGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT           96 a           74 c           39 g          104 t
ORIGIN      
        1 attacctcac tattaattgt atttcaaact aacaatcgca ctccattttc tctttttaaa
       61 aaacataaac actgttaaaa caaatactta tttcgttatc ttaaatgcta tttctacatc
      121 actcgtcctt ataatcaaat tattcttatt catagctatt tttgcaggtt taaaatagcg
      181 gctttcacca aagtcagcca tgaaaattag actacaagtc agggcaagat tacgaaagcc
      241 cgctccccgc aaggactgac gccagatagt ttctgtccat ggctgctttt cgcatcttac
      301 gtcttaaccc tgc
//
LOCUS       DAZPLM010000564          309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZPLM010000564 DAZPLM010000000
VERSION     DAZPLM010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.569"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>309
                     /locus_tag="ACHVC7_004642"
     CDS             <1..>309
                     /locus_tag="ACHVC7_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2118453.1"
                     /translation="NDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQD
                     VAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATM
                     E"
BASE COUNT           83 a           80 c          101 g           45 t
ORIGIN      
        1 aatgactttc tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt
       61 gaggaaatgg tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag
      121 acggcgcagg atgtggcggc agccgcaggt tatgcccgcg cagcagagca ggccaaaaat
      181 gacattgatg ctgcgctgac cggcactctg aaaacggcta accatctgtc tgaaatcgca
      241 gcagcaggcg aaaaggcaca acagaagtcc cgggataatc tggggctgaa aagtgcggcc
      301 acgatggaa
//
LOCUS       DAZPLM010000565          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZPLM010000565 DAZPLM010000000
VERSION     DAZPLM010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.570"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHVC7_004643"
     CDS             <1..>301
                     /locus_tag="ACHVC7_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118454.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPLM010000566          299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZPLM010000566 DAZPLM010000000
VERSION     DAZPLM010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.571"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHVC7_004644"
     CDS             complement(<1..242)
                     /locus_tag="ACHVC7_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR2118455.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           87 a           58 c           63 g           91 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagt
//
LOCUS       DAZPLM010000567          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZPLM010000567 DAZPLM010000000
VERSION     DAZPLM010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.572"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
BASE COUNT          111 a           45 c           46 g           96 t
ORIGIN      
        1 tataaaaaat gtttattact cgatgtaagg atacattaat tttacgttta ttatgatgaa
       61 tcctctgcaa atgagcgaaa tttgaaagaa catttatctg ctgtacgatt taatgaactt
      121 aatgatacag cattacaaaa acaatcgaag tttataaaga tgattcctga ttgaccagaa
      181 ccctatccta caatggtgac taattttatt actcccgatg gagatgatga tatgaatata
      241 tcatatgtga atagcaacaa aacgacatct ttacctgttg agctagatgc actaaata
//
LOCUS       DAZPLM010000568          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZPLM010000568 DAZPLM010000000
VERSION     DAZPLM010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.573"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHVC7_004645"
     CDS             <1..>292
                     /locus_tag="ACHVC7_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR2118456.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZPLM010000569          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZPLM010000569 DAZPLM010000000
VERSION     DAZPLM010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.574"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>291
                     /locus_tag="ACHVC7_004646"
     CDS             <1..>291
                     /locus_tag="ACHVC7_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR2118457.1"
                     /translation="QGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKN
                     EWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSN"
BASE COUNT           87 a           66 c           80 g           58 t
ORIGIN      
        1 atcagggcag tcattatacg agcaggcagt tccggcagtt attgtggcga taccagatca
       61 gacagagtat gagccggcgc ggaaactgct gggataacag cccaatggaa cgcttcttca
      121 ggagtctgaa gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc
      181 acgccataac ggactatatc gttggatatt acagcgcact aagaccgcac gaatataacg
      241 gtgggttacc cccaaacgaa tcggaaaatc gatactggaa aaactctaac t
//
LOCUS       DAZPLM010000570          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZPLM010000570 DAZPLM010000000
VERSION     DAZPLM010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.576"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            <1..>284
                     /locus_tag="ACHVC7_004647"
     CDS             <1..>284
                     /locus_tag="ACHVC7_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR2118458.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           88 g           69 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgc
//
LOCUS       DAZPLM010000571          280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZPLM010000571 DAZPLM010000000
VERSION     DAZPLM010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.denovo.577"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            complement(<1..>280)
                     /locus_tag="ACHVC7_004648"
     CDS             complement(<1..>280)
                     /locus_tag="ACHVC7_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118459.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKE"
BASE COUNT           87 a           60 c           47 g           86 t
ORIGIN      
        1 ttcttttaca tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac
       61 agtatctgaa tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg
      121 tgatgcattg tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt
      181 tctcaatata atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa
      241 tccatctgta tccaagtcaa cgtcattatc tgcagatgtt
//
LOCUS       DAZPLM010000572         6065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.guided.232, whole genome shotgun sequence.
ACCESSION   DAZPLM010000572 DAZPLM010000000
VERSION     DAZPLM010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.guided.232"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            545..1360
                     /gene="sul2"
                     /locus_tag="ACHVC7_004649"
     CDS             545..1360
                     /gene="sul2"
                     /locus_tag="ACHVC7_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR2118460.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1447..1749
                     /locus_tag="ACHVC7_004650"
     CDS             1447..1749
                     /locus_tag="ACHVC7_004650"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118461.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1925..3418)
                     /locus_tag="ACHVC7_004651"
     CDS             complement(1925..3418)
                     /locus_tag="ACHVC7_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR2118462.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3462..3851)
                     /locus_tag="ACHVC7_004652"
     CDS             complement(3462..3851)
                     /locus_tag="ACHVC7_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118463.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4069..4509
                     /locus_tag="ACHVC7_004653"
     CDS             4069..4509
                     /locus_tag="ACHVC7_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR2118464.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5232..5381)
                     /locus_tag="ACHVC7_004654"
     CDS             complement(5232..5381)
                     /locus_tag="ACHVC7_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2118465.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            5952..>6065
                     /locus_tag="ACHVC7_004655"
     CDS             5952..>6065
                     /locus_tag="ACHVC7_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2118466.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNR"
BASE COUNT         1443 a         1675 c         1590 g         1357 t
ORIGIN      
        1 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
       61 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
      121 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
      181 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
      241 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
      301 actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
      361 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
      421 aaatgctaca gatacatcat catgaaacgg atcaccgcaa acaggttact cgcctgggga
      481 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
      541 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
      601 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
      661 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
      721 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
      781 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
      841 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
      901 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
      961 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     1021 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     1081 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     1141 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     1201 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     1261 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     1321 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     1381 tctatattcg cggttcagca ggcatgtccc ctttgagggc gacccgacga caggataatc
     1441 gaccttatgg tgcgcaaata tttcggcaca gacggtattc gtggcaaagc caacgaaggc
     1501 gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg ctggccgtgt ctttcgtcgc
     1561 ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc gcctgtcggg ctatatgctt
     1621 gaacccgcgc tcacagccgg tttcacctcg atgggcatgg acgtattcct ttttggcccg
     1681 ctgccgacaa cgtataggaa gaataaacgc ccttttcacc caagtccaac agctttggac
     1741 cgcagttgac tctttcgaca cccctgcgat gcaacccaat ccggctgacg gggagccagc
     1801 aacgctgaaa atttaccctc ctctttccca ctagcggctc cttttccgac aaccagcacg
     1861 gcggatccct gccgcggcgc tgtgaacgca gcattttgat tggtatcgtt ggccttcagg
     1921 ctcgtcagtc aaacagaccc aggagcagct cagccggtgg cgcccggctt tcgggtaacg
     1981 ccctggtccc gctggtttcg gctttgtgct tcaggtgatc aaggatctgc ttgatcacta
     2041 tagggtcttc aatgcaggcg atgactttca tggcgccgcc gcagccgctg caggtctcga
     2101 tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt catcgacgct cgccgttgtg
     2161 ctggtgttgc cggttcatca gccaccctga ccttgttgcc cctgccccgt tttgccggcg
     2221 tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc gtggaagcgg gttaggttga
     2281 ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa atccaatggt tcgaaaatga
     2341 cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta gcgcacgttg ccgcctcgtg
     2401 ttaacgacag ccgcttctcg gataccgccg ggcggctgat gtaccggcac agccgttcga
     2461 gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg caggctggac ccggctacct
     2521 tgccaatccc gtcaccgaac ggatcaccac tggtcggcag agtttgcaaa gtgaacacct
     2581 ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat cgagtgcccc agcaggggtg
     2641 tcatcgggtc gtcatccacc gcatccgagg ccagatagct gttttcgaca tcccgttcca
     2701 gcaggccttg ccgttccaga tagcgaccca cccggtgggc gatggtgtgc gtcagctggg
     2761 tgagctctgg gctggtcggc gccttgaccc agcggaaacg cgctgagccg tgggattgct
     2821 cgacatacac accgtcgaga aacagcatgt ggaagtgaac attcagattg agcgccgatc
     2881 caaaacgctg gatcagggtg accgcgcccg tcttggccac ttggtgggta tggcccgctt
     2941 tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc cagcacccac cccatgatct
     3001 cgggccggct ggcaaacagg aaacgcagct gaaacgggaa gctcaacacc cactgacgca
     3061 tgggttgttc aggcagtact tcatcaacca gcaaggcggc actttcggcc atccgccgcg
     3121 ccccacagct cgggcagaaa ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt
     3181 ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt
     3241 cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag
     3301 cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc
     3361 tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt
     3421 tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
     3481 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccact
     3541 cttacattgt tcgcagtatc taggtacatg ttattgcgtg caccttcatg atgtataatg
     3601 ttattaatga cagcagaaag atcgacagaa ttaatattct tcgtacctgg gacagttatc
     3661 gggtgtcggc cagtgatgag accaaaggat gcagtataag atgaaccact actattatca
     3721 tggccaccat taccaccacc gttaaaacta cctcccgaac ttccagagcc gctatgacca
     3781 tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac cgacagtacc gctatttaac
     3841 tctgtactca taaaattatc ctctttgact tttaaaaaca gcaaaaactg tatttataac
     3901 aatgtgttta tatacagtat tcgaaatact acgcccgtca aaaccacacc gtcaacaggt
     3961 tttttgtgta tttttcacgc ctcacgggca gcctcagacc ttttgtgtta aaacagtaat
     4021 atgttctgct gttacaatac ggtacacaca ggagacactg tcacttttat ggcatggcac
     4081 acagttcaag agacactgaa attaacagga aagtcgcgct cgcaattata tcgtgacatg
     4141 gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc gccgggagtt cgagaccagt
     4201 gaactgattc gggcctatgg cgagctgcgc acttctgaga caccaaaaag gcacaaggag
     4261 ggacactcca aatctgtcgt tagccaggcg gaaaatgctc agctagacgt cattcagcag
     4321 aaactggacg ctctgcagca gactgttacc ctaatgcttg aagacaagat ggcgcgggaa
     4381 gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac aggccgaaat tagccgtctg
     4441 gctgacgcgc tggcacagga aaaaaagcgg ggattctggt cgcggctgtt tcgctccggt
     4501 tcagagtaac agtgcaggtg actctgattt accataacat ctgttatcag caccgcctga
     4561 gaggccctca gggcgtttct gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa
     4621 cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc acaagtgcat
     4681 agcgcggaaa ttcacaccat tcagccgggg taagtcctgt ttccggcgtt cttcccatcc
     4741 gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg
     4801 gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta caaatggcac agaggatgaa
     4861 agtgacgtat gagctgaagg aatgatgtaa aagtgatgta tgaactgaag gagcgatgta
     4921 attctgtagt caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc
     4981 tttttttgtc acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt
     5041 caacctcaaa atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt
     5101 aaaagaaaaa aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact
     5161 gtaattacat gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc
     5221 aagcgaaaaa gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc
     5281 aggttctctt cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc
     5341 gtatcagtcg aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct
     5401 gagaggttgt tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag
     5461 tgtatactgg cttactatgt tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca
     5521 ggagaaaaaa ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt
     5581 cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg
     5641 gacggggacg gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg
     5701 ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac
     5761 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
     5821 gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     5881 tatggccgcg gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca
     5941 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact
     6001 atcaacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta
     6061 acagg
//
LOCUS       DAZPLM010000573         5354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.guided.251, whole genome shotgun sequence.
ACCESSION   DAZPLM010000573 DAZPLM010000000
VERSION     DAZPLM010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.guided.251"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHVC7_004656"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHVC7_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHVC7_004657"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHVC7_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR2118467.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHVC7_004658"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHVC7_004658"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR2118468.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHVC7_004659"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHVC7_004659"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR2118469.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHVC7_004660"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHVC7_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR2118470.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHVC7_004661"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHVC7_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR2118471.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         1295 a         1314 c         1435 g         1310 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aacc
//
LOCUS       DAZPLM010000574         3229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2100437
            SAMN43277593-rid23488923.guided.310, whole genome shotgun sequence.
ACCESSION   DAZPLM010000574 DAZPLM010000000
VERSION     DAZPLM010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277593
            Sequence Read Archive: SRR30464308
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396659.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 123 of 269
            Pseudo Genes (incomplete)         :: 90 of 269
            Pseudo Genes (internal stop)      :: 86 of 269
            Pseudo Genes (multiple problems)  :: 29 of 269
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277593-rid23488923.guided.310"
                     /strain="M2100437"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-03"
                     /collected_by="FSS"
     gene            26..1159
                     /locus_tag="ACHVC7_004662"
     CDS             26..1159
                     /locus_tag="ACHVC7_004662"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR2118472.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /gene="blc"
                     /locus_tag="ACHVC7_004663"
     CDS             1248..1781
                     /gene="blc"
                     /locus_tag="ACHVC7_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR2118473.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHVC7_004664"
     CDS             complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHVC7_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR2118474.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHVC7_004665"
     CDS             complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHVC7_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR2118475.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHVC7_004666"
     CDS             complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHVC7_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR2118476.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2704..3228)
                     /gene="efp"
                     /locus_tag="ACHVC7_004667"
     CDS             complement(2704..3228)
                     /gene="efp"
                     /locus_tag="ACHVC7_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR2118477.1"
                     /translation="MMLDGEPYAVEASEFVKPGKGQAFARVKLRRLLTGTRVEKTFKS
                     TDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGDNAKWLLDQAECIVT
                     LWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGAVVKVPLFVQIGEVI
                     KVDTRSGEYVSRVK"
BASE COUNT          837 a          829 c          797 g          766 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 tactgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1321 cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg ctataaccct gacagagaaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac agtaataaca cttggcgtca aacgactaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaga attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttgtga
     2221 ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac cgttattgct tattgctgcg
     2281 ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga aatgtcttca ccaacgccac
     2341 gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag atcgctgcaa
     2401 ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt gcctatagca
     2461 aattaataat agacaacatc gccaaaaatg atggaaatct gatgattttt cggaagagaa
     2521 gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt cttcgccgaa
     2581 accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa gaacgataag
     2641 gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg caccacaacc
     2701 gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga tgacttcgcc
     2761 gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag ccggtttgcc
     2821 gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt ccagttcaac
     2881 gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga tacactctgc
     2941 ctgatccagc agccatttag cgttgtcgcc aattgctttt gcatcagcag acagctgctc
     3001 gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt
     3061 catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt tttctacgcg
     3121 agtaccggtc agcagacgac gcagtttaac gcgagcaaat gcctggcctt tacccggttt
     3181 tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct aacatgatt
//
